LOCUS       DAZOXR010000001        81540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZOXR010000001 DAZOXR010000000
VERSION     DAZOXR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 81540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.001"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(127..936)
                     /gene="pduF"
                     /locus_tag="ACHUNN_000001"
     CDS             complement(127..936)
                     /gene="pduF"
                     /locus_tag="ACHUNN_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR0574507.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            1448..1732
                     /gene="pduA"
                     /locus_tag="ACHUNN_000002"
     CDS             1448..1732
                     /gene="pduA"
                     /locus_tag="ACHUNN_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR0574508.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            1729..2541
                     /gene="pduB"
                     /locus_tag="ACHUNN_000003"
     CDS             1729..2541
                     /gene="pduB"
                     /locus_tag="ACHUNN_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFR0574509.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            2560..4224
                     /gene="pduC"
                     /locus_tag="ACHUNN_000004"
     CDS             2560..4224
                     /gene="pduC"
                     /locus_tag="ACHUNN_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR0574510.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            4234..4902
                     /locus_tag="ACHUNN_000005"
     CDS             4234..4902
                     /locus_tag="ACHUNN_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR0574511.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            4917..5435
                     /gene="pduE"
                     /locus_tag="ACHUNN_000006"
     CDS             4917..5435
                     /gene="pduE"
                     /locus_tag="ACHUNN_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR0574512.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            5445..7277
                     /locus_tag="ACHUNN_000007"
     CDS             5445..7277
                     /locus_tag="ACHUNN_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR0574513.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            7267..7617
                     /locus_tag="ACHUNN_000008"
     CDS             7267..7617
                     /locus_tag="ACHUNN_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR0574514.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            7637..7912
                     /gene="pduJ"
                     /locus_tag="ACHUNN_000009"
     CDS             7637..7912
                     /gene="pduJ"
                     /locus_tag="ACHUNN_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR0574515.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            7936..8358
                     /locus_tag="ACHUNN_000010"
     CDS             7936..8358
                     /locus_tag="ACHUNN_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR0574516.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            8361..8993
                     /locus_tag="ACHUNN_000011"
     CDS             8361..8993
                     /locus_tag="ACHUNN_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR0574517.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            8990..9481
                     /gene="pduM"
                     /locus_tag="ACHUNN_000012"
     CDS             8990..9481
                     /gene="pduM"
                     /locus_tag="ACHUNN_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR0574518.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            9485..9760
                     /gene="pduN"
                     /locus_tag="ACHUNN_000013"
     CDS             9485..9760
                     /gene="pduN"
                     /locus_tag="ACHUNN_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR0574519.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            9772..10779
                     /gene="pduO"
                     /locus_tag="ACHUNN_000014"
     CDS             9772..10779
                     /gene="pduO"
                     /locus_tag="ACHUNN_000014"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR0574520.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            10776..12161
                     /gene="pduP"
                     /locus_tag="ACHUNN_000015"
                     /pseudo
     CDS             10776..12161
                     /gene="pduP"
                     /locus_tag="ACHUNN_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            12172..13284
                     /locus_tag="ACHUNN_000016"
     CDS             12172..13284
                     /locus_tag="ACHUNN_000016"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR0574521.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            13281..14626
                     /locus_tag="ACHUNN_000017"
                     /pseudo
     CDS             13281..14626
                     /locus_tag="ACHUNN_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            14629..15183
                     /gene="pduT"
                     /locus_tag="ACHUNN_000018"
     CDS             14629..15183
                     /gene="pduT"
                     /locus_tag="ACHUNN_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR0574522.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            15183..15533
                     /gene="pduU"
                     /locus_tag="ACHUNN_000019"
     CDS             15183..15533
                     /gene="pduU"
                     /locus_tag="ACHUNN_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR0574523.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            15538..15981
                     /locus_tag="ACHUNN_000020"
     CDS             15538..15981
                     /locus_tag="ACHUNN_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0574524.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(16082..16411)
                     /locus_tag="ACHUNN_000021"
     CDS             complement(16082..16411)
                     /locus_tag="ACHUNN_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0574525.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(16583..17641)
                     /locus_tag="ACHUNN_000022"
     CDS             complement(16583..17641)
                     /locus_tag="ACHUNN_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0574526.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(17839..18312)
                     /gene="sbmC"
                     /locus_tag="ACHUNN_000023"
     CDS             complement(17839..18312)
                     /gene="sbmC"
                     /locus_tag="ACHUNN_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR0574527.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(18431..19597)
                     /gene="dacD"
                     /locus_tag="ACHUNN_000024"
     CDS             complement(18431..19597)
                     /gene="dacD"
                     /locus_tag="ACHUNN_000024"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR0574528.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            19806..21233
                     /gene="sbcB"
                     /locus_tag="ACHUNN_000025"
     CDS             19806..21233
                     /gene="sbcB"
                     /locus_tag="ACHUNN_000025"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0574529.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(21276..21503)
                     /gene="tsuB"
                     /locus_tag="ACHUNN_000026"
     CDS             complement(21276..21503)
                     /gene="tsuB"
                     /locus_tag="ACHUNN_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0574530.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(21517..22575)
                     /gene="tsuA"
                     /locus_tag="ACHUNN_000027"
     CDS             complement(21517..22575)
                     /gene="tsuA"
                     /locus_tag="ACHUNN_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0574531.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(22754..24112)
                     /gene="plaP"
                     /locus_tag="ACHUNN_000028"
     CDS             complement(22754..24112)
                     /gene="plaP"
                     /locus_tag="ACHUNN_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0574532.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(24102..24164)
                     /gene="yoeI"
                     /locus_tag="ACHUNN_000029"
     CDS             complement(24102..24164)
                     /gene="yoeI"
                     /locus_tag="ACHUNN_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0574533.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(24379..25308)
                     /locus_tag="ACHUNN_000030"
     CDS             complement(24379..25308)
                     /locus_tag="ACHUNN_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0574534.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(25354..26178)
                     /locus_tag="ACHUNN_000031"
     CDS             complement(25354..26178)
                     /locus_tag="ACHUNN_000031"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0574535.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(26261..26515)
                     /gene="yoeB"
                     /locus_tag="ACHUNN_000032"
     CDS             complement(26261..26515)
                     /gene="yoeB"
                     /locus_tag="ACHUNN_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0574536.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(26512..26763)
                     /gene="yefM"
                     /locus_tag="ACHUNN_000033"
     CDS             complement(26512..26763)
                     /gene="yefM"
                     /locus_tag="ACHUNN_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0574537.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            27046..27096
                     /gene="hisL"
                     /locus_tag="ACHUNN_000034"
     CDS             27046..27096
                     /gene="hisL"
                     /locus_tag="ACHUNN_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR0574538.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    27072..27195
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            27242..28141
                     /gene="hisG"
                     /locus_tag="ACHUNN_000035"
     CDS             27242..28141
                     /gene="hisG"
                     /locus_tag="ACHUNN_000035"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0574539.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            28147..29451
                     /gene="hisD"
                     /locus_tag="ACHUNN_000036"
     CDS             28147..29451
                     /gene="hisD"
                     /locus_tag="ACHUNN_000036"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0574540.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            29448..30518
                     /gene="hisC"
                     /locus_tag="ACHUNN_000037"
     CDS             29448..30518
                     /gene="hisC"
                     /locus_tag="ACHUNN_000037"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0574541.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            30518..31585
                     /gene="hisB"
                     /locus_tag="ACHUNN_000038"
     CDS             30518..31585
                     /gene="hisB"
                     /locus_tag="ACHUNN_000038"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0574542.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            31585..32175
                     /gene="hisH"
                     /locus_tag="ACHUNN_000039"
     CDS             31585..32175
                     /gene="hisH"
                     /locus_tag="ACHUNN_000039"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0574543.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            32175..32912
                     /gene="hisA"
                     /locus_tag="ACHUNN_000040"
     CDS             32175..32912
                     /gene="hisA"
                     /locus_tag="ACHUNN_000040"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0574544.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            32894..33670
                     /gene="hisF"
                     /locus_tag="ACHUNN_000041"
     CDS             32894..33670
                     /gene="hisF"
                     /locus_tag="ACHUNN_000041"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0574545.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            33664..34275
                     /gene="hisIE"
                     /locus_tag="ACHUNN_000042"
     CDS             33664..34275
                     /gene="hisIE"
                     /locus_tag="ACHUNN_000042"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0574546.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(34372..35349)
                     /gene="wzzB"
                     /locus_tag="ACHUNN_000043"
     CDS             complement(34372..35349)
                     /gene="wzzB"
                     /locus_tag="ACHUNN_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0574547.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(35495..36661)
                     /gene="ugd"
                     /locus_tag="ACHUNN_000044"
     CDS             complement(35495..36661)
                     /gene="ugd"
                     /locus_tag="ACHUNN_000044"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR0574548.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(36911..38317)
                     /gene="gndA"
                     /locus_tag="ACHUNN_000045"
     CDS             complement(36911..38317)
                     /gene="gndA"
                     /locus_tag="ACHUNN_000045"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0574549.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(38481..39851)
                     /gene="cpsG"
                     /locus_tag="ACHUNN_000046"
                     /gene_synonym="manB"
     CDS             complement(38481..39851)
                     /gene="cpsG"
                     /locus_tag="ACHUNN_000046"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0574550.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(39956..41392)
                     /gene="cpsB"
                     /locus_tag="ACHUNN_000047"
     CDS             complement(39956..41392)
                     /gene="cpsB"
                     /locus_tag="ACHUNN_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0574551.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(41395..42618)
                     /gene="wcaI"
                     /locus_tag="ACHUNN_000048"
     CDS             complement(41395..42618)
                     /gene="wcaI"
                     /locus_tag="ACHUNN_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0574552.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(42615..43094)
                     /gene="gmm"
                     /locus_tag="ACHUNN_000049"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(42615..43094)
                     /gene="gmm"
                     /locus_tag="ACHUNN_000049"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0574553.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(43097..44062)
                     /gene="fcl"
                     /locus_tag="ACHUNN_000050"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(43097..44062)
                     /gene="fcl"
                     /locus_tag="ACHUNN_000050"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0574554.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(44065..45186)
                     /gene="gmd"
                     /locus_tag="ACHUNN_000051"
     CDS             complement(44065..45186)
                     /gene="gmd"
                     /locus_tag="ACHUNN_000051"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0574555.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(45212..45760)
                     /gene="wcaF"
                     /locus_tag="ACHUNN_000052"
     CDS             complement(45212..45760)
                     /gene="wcaF"
                     /locus_tag="ACHUNN_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0574556.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(45776..46522)
                     /gene="wcaE"
                     /locus_tag="ACHUNN_000053"
     CDS             complement(45776..46522)
                     /gene="wcaE"
                     /locus_tag="ACHUNN_000053"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0574557.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(46533..47750)
                     /gene="wcaD"
                     /locus_tag="ACHUNN_000054"
     CDS             complement(46533..47750)
                     /gene="wcaD"
                     /locus_tag="ACHUNN_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR0574558.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(47725..48942)
                     /gene="wcaC"
                     /locus_tag="ACHUNN_000055"
     CDS             complement(47725..48942)
                     /gene="wcaC"
                     /locus_tag="ACHUNN_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0574559.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(48939..49427)
                     /gene="wcaB"
                     /locus_tag="ACHUNN_000056"
     CDS             complement(48939..49427)
                     /gene="wcaB"
                     /locus_tag="ACHUNN_000056"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0574560.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(49430..50269)
                     /gene="wcaA"
                     /locus_tag="ACHUNN_000057"
     CDS             complement(49430..50269)
                     /gene="wcaA"
                     /locus_tag="ACHUNN_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR0574561.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(50362..52524)
                     /gene="wzc"
                     /locus_tag="ACHUNN_000058"
     CDS             complement(50362..52524)
                     /gene="wzc"
                     /locus_tag="ACHUNN_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0574562.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(52527..52970)
                     /gene="wzb"
                     /locus_tag="ACHUNN_000059"
     CDS             complement(52527..52970)
                     /gene="wzb"
                     /locus_tag="ACHUNN_000059"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0574563.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(52976..54115)
                     /gene="wza"
                     /locus_tag="ACHUNN_000060"
     CDS             complement(52976..54115)
                     /gene="wza"
                     /locus_tag="ACHUNN_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0574564.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            54424..54573
                     /locus_tag="ACHUNN_000061"
     CDS             54424..54573
                     /locus_tag="ACHUNN_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0574565.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            54774..56357
                     /locus_tag="ACHUNN_000062"
     CDS             54774..56357
                     /locus_tag="ACHUNN_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0574566.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(56631..58484)
                     /gene="asmA"
                     /locus_tag="ACHUNN_000063"
     CDS             complement(56631..58484)
                     /gene="asmA"
                     /locus_tag="ACHUNN_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0574567.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(58506..59087)
                     /gene="dcd"
                     /locus_tag="ACHUNN_000064"
     CDS             complement(58506..59087)
                     /gene="dcd"
                     /locus_tag="ACHUNN_000064"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0574568.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(59179..59820)
                     /gene="udk"
                     /locus_tag="ACHUNN_000065"
     CDS             complement(59179..59820)
                     /gene="udk"
                     /locus_tag="ACHUNN_000065"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0574569.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            60138..63455
                     /locus_tag="ACHUNN_000066"
     CDS             60138..63455
                     /locus_tag="ACHUNN_000066"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0574570.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(63565..64413)
                     /gene="alkA"
                     /locus_tag="ACHUNN_000067"
     CDS             complement(63565..64413)
                     /gene="alkA"
                     /locus_tag="ACHUNN_000067"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR0574571.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            64547..65899
                     /gene="yegD"
                     /locus_tag="ACHUNN_000068"
     CDS             64547..65899
                     /gene="yegD"
                     /locus_tag="ACHUNN_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0574572.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(65912..67852)
                     /gene="yegI"
                     /locus_tag="ACHUNN_000069"
     CDS             complement(65912..67852)
                     /gene="yegI"
                     /locus_tag="ACHUNN_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR0574573.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(67849..68610)
                     /gene="pphC"
                     /locus_tag="ACHUNN_000070"
     CDS             complement(67849..68610)
                     /gene="pphC"
                     /locus_tag="ACHUNN_000070"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR0574574.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(68607..69266)
                     /locus_tag="ACHUNN_000071"
     CDS             complement(68607..69266)
                     /locus_tag="ACHUNN_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0574575.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(69487..69546)
                     /gene="ibsA"
                     /locus_tag="ACHUNN_000072"
     CDS             complement(69487..69546)
                     /gene="ibsA"
                     /locus_tag="ACHUNN_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR0574576.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(69819..69875)
                     /locus_tag="ACHUNN_000073"
     CDS             complement(69819..69875)
                     /locus_tag="ACHUNN_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0574577.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(70146..70202)
                     /locus_tag="ACHUNN_000074"
     CDS             complement(70146..70202)
                     /locus_tag="ACHUNN_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0574578.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            70481..71722
                     /gene="mdtA"
                     /locus_tag="ACHUNN_000075"
     CDS             70481..71722
                     /gene="mdtA"
                     /locus_tag="ACHUNN_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR0574579.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            71722..74844
                     /gene="mdtB"
                     /locus_tag="ACHUNN_000076"
     CDS             71722..74844
                     /gene="mdtB"
                     /locus_tag="ACHUNN_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR0574580.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            74845..77922
                     /gene="mdtC"
                     /locus_tag="ACHUNN_000077"
     CDS             74845..77922
                     /gene="mdtC"
                     /locus_tag="ACHUNN_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0574581.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            77923..79338
                     /locus_tag="ACHUNN_000078"
     CDS             77923..79338
                     /locus_tag="ACHUNN_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0574582.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            79335..80738
                     /gene="baeS"
                     /locus_tag="ACHUNN_000079"
     CDS             79335..80738
                     /gene="baeS"
                     /locus_tag="ACHUNN_000079"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0574583.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            80735..81457
                     /gene="baeR"
                     /locus_tag="ACHUNN_000080"
     CDS             80735..81457
                     /gene="baeR"
                     /locus_tag="ACHUNN_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0574584.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        19811 a        21550 c        20723 g        19456 t
ORIGIN      
        1 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
       61 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
      121 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
      181 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
      241 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
      301 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
      361 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
      421 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
      481 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
      541 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
      601 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
      661 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
      721 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
      781 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
      841 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
      901 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
      961 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     1021 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     1081 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     1141 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     1201 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     1261 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     1321 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     1381 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     1441 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     1501 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     1561 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     1621 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     1681 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     1741 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     1801 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     1861 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     1921 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     1981 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     2041 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     2101 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     2161 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     2221 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     2281 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     2341 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     2401 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     2461 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     2521 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     2581 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     2641 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     2701 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     2761 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     2821 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     2881 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     2941 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     3001 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     3061 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     3121 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     3181 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     3241 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     3301 tcctatgctt ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag
     3361 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     3421 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
     3481 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
     3541 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
     3601 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
     3661 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
     3721 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
     3781 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
     3841 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
     3901 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
     3961 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
     4021 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
     4081 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
     4141 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
     4201 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
     4261 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
     4321 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
     4381 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
     4441 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
     4501 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
     4561 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
     4621 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
     4681 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
     4741 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
     4801 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
     4861 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
     4921 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
     4981 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
     5041 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
     5101 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
     5161 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
     5221 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
     5281 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
     5341 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
     5401 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
     5461 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
     5521 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
     5581 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
     5641 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
     5701 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
     5761 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
     5821 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
     5881 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
     5941 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
     6001 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
     6061 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
     6121 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
     6181 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
     6241 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
     6301 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
     6361 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
     6421 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
     6481 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
     6541 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
     6601 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
     6661 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
     6721 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
     6781 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
     6841 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
     6901 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
     6961 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
     7021 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
     7081 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
     7141 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
     7201 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
     7261 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
     7321 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
     7381 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
     7441 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
     7501 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
     7561 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
     7621 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
     7681 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
     7741 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
     7801 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
     7861 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
     7921 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
     7981 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
     8041 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
     8101 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
     8161 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
     8221 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
     8281 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
     8341 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
     8401 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
     8461 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
     8521 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
     8581 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
     8641 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
     8701 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
     8761 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
     8821 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
     8881 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
     8941 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
     9001 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
     9061 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
     9121 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
     9181 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
     9241 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
     9301 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
     9361 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
     9421 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
     9481 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
     9541 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
     9601 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
     9661 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
     9721 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
     9781 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
     9841 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
     9901 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
     9961 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    10021 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    10081 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    10141 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    10201 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    10261 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    10321 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    10381 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    10441 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    10501 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    10561 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    10621 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    10681 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    10741 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    10801 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    10861 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    10921 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    10981 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    11041 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    11101 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    11161 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    11221 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    11281 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    11341 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    11401 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    11461 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    11521 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    11581 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    11641 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    11701 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    11761 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    11821 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    11881 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    11941 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    12001 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    12061 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    12121 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    12181 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    12241 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    12301 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    12361 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    12421 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    12481 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    12541 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    12601 cttttcgata atgcacttta tccggatatc gcgatccttg atccatcttt ggtcgtcagc
    12661 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    12721 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    12781 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    12841 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    12901 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    12961 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    13021 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    13081 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    13141 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    13201 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    13261 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    13321 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    13381 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    13441 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    13501 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    13561 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    13621 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    13681 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    13741 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    13801 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    13861 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    13921 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    13981 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    14041 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    14101 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    14161 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    14221 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    14281 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    14341 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    14401 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    14461 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    14521 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    14581 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    14641 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    14701 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    14761 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    14821 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    14881 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    14941 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    15001 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    15061 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    15121 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    15181 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    15241 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    15301 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    15361 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    15421 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    15481 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    15541 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    15601 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    15661 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    15721 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    15781 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    15841 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    15901 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    15961 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    16021 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    16081 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    16141 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    16201 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    16261 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    16321 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    16381 cgtcctgaaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    16441 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    16501 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    16561 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    16621 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    16681 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    16741 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    16801 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    16861 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    16921 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    16981 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    17041 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    17101 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    17161 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    17221 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    17281 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    17341 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    17401 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    17461 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    17521 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    17581 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    17641 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    17701 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    17761 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    17821 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    17881 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    17941 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    18001 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    18061 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    18121 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    18181 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    18241 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    18301 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    18361 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    18421 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    18481 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    18541 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    18601 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    18661 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    18721 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    18781 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    18841 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    18901 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    18961 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    19021 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    19081 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    19141 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    19201 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    19261 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    19321 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    19381 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    19441 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    19501 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    19561 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    19621 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    19681 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    19741 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    19801 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    19861 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    19921 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    19981 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    20041 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    20101 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    20161 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    20221 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    20281 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    20341 acgcccacga tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa
    20401 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    20461 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    20521 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    20581 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    20641 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    20701 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    20761 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    20821 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    20881 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    20941 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    21001 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    21061 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    21121 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    21181 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    21241 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    21301 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    21361 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    21421 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    21481 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    21541 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    21601 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    21661 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    21721 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    21781 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    21841 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    21901 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    21961 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    22021 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    22081 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    22141 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    22201 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    22261 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    22321 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    22381 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    22441 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    22501 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    22561 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    22621 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    22681 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    22741 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    22801 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    22861 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    22921 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    22981 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    23041 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    23101 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    23161 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    23221 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    23281 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    23341 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    23401 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    23461 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    23521 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    23581 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    23641 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    23701 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    23761 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    23821 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    23881 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    23941 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    24001 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    24061 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    24121 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    24181 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    24241 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    24301 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    24361 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    24421 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    24481 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    24541 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    24601 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    24661 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    24721 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    24781 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    24841 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    24901 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    24961 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    25021 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    25081 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    25141 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    25201 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    25261 tttttctaag gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt
    25321 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    25381 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    25441 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    25501 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    25561 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    25621 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    25681 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    25741 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    25801 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    25861 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    25921 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    25981 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    26041 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    26101 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    26161 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    26221 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    26281 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    26341 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    26401 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    26461 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    26521 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    26581 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    26641 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    26701 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    26761 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    26821 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    26881 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    26941 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    27001 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    27061 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    27121 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    27181 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    27241 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    27301 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    27361 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    27421 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    27481 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    27541 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    27601 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    27661 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    27721 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    27781 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    27841 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    27901 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    27961 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    28021 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    28081 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    28141 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    28201 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    28261 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    28321 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    28381 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    28441 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    28501 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    28561 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    28621 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    28681 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    28741 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    28801 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    28861 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    28921 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    28981 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    29041 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    29101 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    29161 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    29221 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    29281 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    29341 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    29401 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    29461 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    29521 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    29581 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    29641 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    29701 acgaaggtat tgaactgctg attcgcgctt tttgcgaatc gggtaaagac gccatcctct
    29761 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    29821 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    29881 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    29941 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    30001 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    30061 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    30121 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    30181 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    30241 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    30301 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    30361 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    30421 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    30481 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    30541 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    30601 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    30661 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    30721 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    30781 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    30841 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    30901 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    30961 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    31021 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    31081 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    31141 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    31201 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    31261 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    31321 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    31381 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    31441 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    31501 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    31561 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    31621 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    31681 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    31741 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    31801 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    31861 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    31921 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    31981 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    32041 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    32101 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac
    32161 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    32221 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    32281 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    32341 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    32401 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    32461 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    32521 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    32581 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    32641 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    32701 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    32761 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    32821 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    32881 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    32941 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    33001 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    33061 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    33121 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    33181 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct
    33241 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    33301 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    33361 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    33421 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    33481 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    33541 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    33601 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    33661 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    33721 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    33781 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    33841 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    33901 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    33961 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    34021 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    34081 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    34141 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    34201 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    34261 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    34321 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    34381 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    34441 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    34501 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    34561 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    34621 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    34681 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    34741 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    34801 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    34861 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    34921 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    34981 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    35041 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    35101 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    35161 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    35221 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcgctag
    35281 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    35341 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    35401 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    35461 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    35521 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    35581 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    35641 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    35701 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    35761 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    35821 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    35881 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    35941 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    36001 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    36061 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    36121 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    36181 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    36241 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    36301 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    36361 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    36421 tggcgatgat gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa
    36481 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    36541 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    36601 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    36661 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    36721 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    36781 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    36841 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    36901 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    36961 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    37021 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    37081 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    37141 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    37201 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    37261 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    37321 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    37381 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    37441 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    37501 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    37561 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    37621 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    37681 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    37741 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    37801 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    37861 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    37921 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    37981 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    38041 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    38101 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    38161 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    38221 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    38281 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    38341 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    38401 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    38461 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    38521 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    38581 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    38641 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aaagggttgc
    38701 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    38761 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    38821 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    38881 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    38941 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    39001 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    39061 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    39121 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    39181 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    39241 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    39301 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    39361 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    39421 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    39481 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    39541 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    39601 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    39661 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    39721 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    39781 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    39841 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    39901 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    39961 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    40021 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    40081 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    40141 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    40201 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    40261 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    40321 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    40381 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    40441 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    40501 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    40561 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    40621 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    40681 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    40741 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    40801 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    40861 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    40921 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    40981 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    41041 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    41101 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    41161 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    41221 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    41281 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    41341 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    41401 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    41461 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    41521 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    41581 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    41641 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    41701 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    41761 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    41821 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    41881 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    41941 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    42001 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    42061 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    42121 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    42181 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    42241 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    42301 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    42361 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    42421 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    42481 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    42541 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    42601 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    42661 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    42721 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    42781 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    42841 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    42901 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    42961 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    43021 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    43081 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    43141 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    43201 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    43261 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    43321 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg
    43381 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    43441 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    43501 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    43561 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    43621 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    43681 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    43741 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    43801 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    43861 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    43921 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    43981 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    44041 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    44101 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    44161 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    44221 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    44281 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    44341 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    44401 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    44461 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    44521 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    44581 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    44641 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    44701 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    44761 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    44821 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    44881 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    44941 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    45001 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    45061 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    45121 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    45181 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    45241 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    45301 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    45361 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    45421 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    45481 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    45541 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    45601 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    45661 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    45721 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    45781 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    45841 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    45901 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    45961 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    46021 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    46081 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    46141 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    46201 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    46261 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    46321 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    46381 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    46441 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    46501 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    46561 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    46621 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    46681 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    46741 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    46801 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    46861 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    46921 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    46981 acaccaaccc ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    47041 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    47101 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    47161 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    47221 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    47281 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    47341 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    47401 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    47461 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    47521 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    47581 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    47641 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    47701 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    47761 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    47821 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    47881 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    47941 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    48001 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    48061 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    48121 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    48181 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    48241 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    48301 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    48361 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    48421 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    48481 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    48541 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    48601 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    48661 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    48721 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    48781 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccataac cgtagacaaa
    48841 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    48901 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    48961 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    49021 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    49081 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    49141 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    49201 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    49261 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    49321 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    49381 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    49441 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    49501 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    49561 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    49621 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    49681 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    49741 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    49801 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    49861 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    49921 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    49981 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    50041 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    50101 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    50161 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    50221 gacggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    50281 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    50341 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    50401 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    50461 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    50521 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    50581 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    50641 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    50701 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    50761 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    50821 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    50881 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    50941 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    51001 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    51061 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    51121 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    51181 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    51241 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    51301 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    51361 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    51421 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    51481 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    51541 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    51601 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    51661 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    51721 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    51781 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    51841 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    51901 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    51961 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    52021 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    52081 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    52141 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    52201 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    52261 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    52321 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    52381 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    52441 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    52501 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    52561 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    52621 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    52681 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    52741 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    52801 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    52861 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    52921 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    52981 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca
    53041 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    53101 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    53161 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    53221 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    53281 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    53341 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    53401 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    53461 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    53521 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    53581 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    53641 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    53701 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    53761 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    53821 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    53881 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    53941 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    54001 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    54061 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    54121 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    54181 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    54241 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    54301 ccgcccctgg ctttttagcc accaacacac tgatttagct taatttttca aaccctctct
    54361 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    54421 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    54481 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    54541 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    54601 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    54661 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    54721 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    54781 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    54841 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    54901 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    54961 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    55021 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    55081 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    55141 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    55201 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    55261 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    55321 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    55381 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    55441 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    55501 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    55561 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    55621 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    55681 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    55741 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    55801 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    55861 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    55921 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    55981 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    56041 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    56101 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    56161 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    56221 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    56281 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    56341 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    56401 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    56461 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    56521 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    56581 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac ctacatcttc
    56641 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    56701 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    56761 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    56821 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    56881 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    56941 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    57001 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    57061 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    57121 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    57181 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    57241 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    57301 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    57361 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    57421 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    57481 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    57541 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    57601 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    57661 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    57721 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    57781 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    57841 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    57901 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    57961 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    58021 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    58081 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    58141 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    58201 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    58261 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    58321 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    58381 atatagtcgc ggaaatcatt cggattcacc agcaacacta acacagataa cccggccacc
    58441 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    58501 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    58561 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    58621 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    58681 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    58741 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    58801 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    58861 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    58921 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    58981 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    59041 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    59101 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    59161 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    59221 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    59281 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    59341 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    59401 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    59461 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    59521 atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa
    59581 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    59641 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    59701 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    59761 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    59821 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    59881 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    59941 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    60001 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    60061 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    60121 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    60181 cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg
    60241 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    60301 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    60361 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    60421 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    60481 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    60541 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    60601 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    60661 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    60721 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    60781 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    60841 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    60901 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    60961 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    61021 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    61081 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    61141 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    61201 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    61261 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    61321 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    61381 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    61441 acgaagcggg cgggattggc atctgggaga gggagttgaa gccgaatgtt tttagctggg
    61501 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    61561 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    61621 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    61681 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    61741 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    61801 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    61861 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    61921 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    61981 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    62041 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    62101 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    62161 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    62221 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    62281 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    62341 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    62401 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    62461 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    62521 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    62581 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    62641 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    62701 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    62761 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    62821 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    62881 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    62941 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    63001 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    63061 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    63121 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    63181 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    63241 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    63301 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    63361 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    63421 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    63481 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    63541 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    63601 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    63661 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    63721 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    63781 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    63841 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    63901 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    63961 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    64021 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    64081 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    64141 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    64201 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    64261 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    64321 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    64381 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    64441 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    64501 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    64561 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    64621 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    64681 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    64741 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    64801 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    64861 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    64921 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    64981 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    65041 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    65101 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    65161 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    65221 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    65281 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    65341 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    65401 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    65461 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    65521 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    65581 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    65641 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    65701 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    65761 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    65821 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    65881 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    65941 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    66001 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    66061 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    66121 tcggcctgaa cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca
    66181 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    66241 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    66301 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    66361 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    66421 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    66481 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    66541 ctttgtacgc tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca
    66601 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    66661 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    66721 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    66781 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    66841 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    66901 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    66961 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    67021 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    67081 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    67141 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    67201 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    67261 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    67321 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    67381 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    67441 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    67501 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    67561 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    67621 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    67681 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    67741 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    67801 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    67861 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    67921 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    67981 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    68041 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    68101 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    68161 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    68221 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    68281 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    68341 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    68401 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    68461 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    68521 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    68581 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    68641 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    68701 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    68761 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    68821 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    68881 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    68941 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    69001 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    69061 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    69121 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    69181 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    69241 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    69301 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    69361 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    69421 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    69481 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    69541 catcataacc ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc
    69601 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    69661 tgaacaaaat agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    69721 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg
    69781 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    69841 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt
    69901 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    69961 ccctgtatgc agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt
    70021 aacgtctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca
    70081 atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg
    70141 tagccttagt aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc
    70201 atgacccttt ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc
    70261 gctgaaatcg gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat
    70321 aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg
    70381 attcccgaca gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt
    70441 atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc
    70501 ccgttgggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    70561 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    70621 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    70681 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac
    70741 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    70801 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    70861 aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    70921 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    70981 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    71041 gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    71101 gcaggttgat gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac
    71161 ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt
    71221 gcaggcgcaa aaagccggaa aaacgctggt ggtagaagcc tgggatcgca ccaattcgaa
    71281 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    71341 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    71401 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct
    71461 gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
    71521 acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    71581 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    71641 agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc
    71701 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    71761 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
    71821 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    71881 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    71941 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    72001 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    72061 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    72121 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    72181 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag
    72241 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct
    72301 gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    72361 cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
    72421 cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta
    72481 atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa
    72541 ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    72601 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg
    72661 ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    72721 accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    72781 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    72841 gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc
    72901 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    72961 gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    73021 ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg
    73081 gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    73141 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    73201 atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc
    73261 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
    73321 aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg
    73381 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact
    73441 ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg
    73501 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    73561 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt
    73621 gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
    73681 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    73741 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    73801 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    73861 caagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    73921 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    73981 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    74041 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    74101 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    74161 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    74221 gagattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag
    74281 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    74341 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    74401 attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt
    74461 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag
    74521 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
    74581 cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg
    74641 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    74701 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc
    74761 accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt
    74821 gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    74881 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
    74941 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    75001 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg
    75061 cgcattgccg gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt
    75121 ttacagtttg attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc
    75181 aacgctgcac aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg
    75241 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    75301 gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    75361 ggtgatgtcg atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    75421 gcgctgttta atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac
    75481 gtgcgtaaac cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat
    75541 gatgagctaa aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc
    75601 gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc
    75661 gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat
    75721 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    75781 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa
    75841 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc
    75901 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    75961 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    76021 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt
    76081 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    76141 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    76201 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
    76261 atttcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    76321 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
    76381 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    76441 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    76501 ccggagcagg acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt
    76561 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    76621 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    76681 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    76741 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    76801 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    76861 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    76921 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    76981 atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt
    77041 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    77101 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    77161 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg
    77221 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    77281 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    77341 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    77401 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    77461 agttacgtac acccactgac gattctctcc accctgccct cggcgggcgt tggtgcgctg
    77521 ctggcgctgg agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta
    77581 ttaatcggca tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa
    77641 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    77701 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    77761 ggtgacggct cggagctgcg gcacctcctg gggatcacca ttgtcggtgg actggtaatg
    77821 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    77881 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac
    77941 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    78001 accatcgtta acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    78061 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    78121 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    78181 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    78241 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    78301 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    78361 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    78421 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg
    78481 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    78541 atggctgtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    78601 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    78661 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    78721 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    78781 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    78841 gtacttggca gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat
    78901 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact
    78961 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    79021 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    79081 gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    79141 accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc
    79201 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    79261 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    79321 aaaaggagcg cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga
    79381 ttttcgccac ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    79441 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    79501 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    79561 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    79621 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    79681 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    79741 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    79801 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt
    79861 tactcgcggc acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    79921 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    79981 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    80041 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    80101 tagcggtgct gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg
    80161 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    80221 atcagttgtc gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga
    80281 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    80341 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    80401 agttattcaa taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    80461 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    80521 gtgtcagtga cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc
    80581 gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    80641 cacataatgg ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    80701 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    80761 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    80821 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta
    80881 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    80941 cttgacgctg tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc
    81001 aaaaatcgaa gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg
    81061 caagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    81121 accgcagcgc gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
    81181 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt
    81241 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    81301 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg
    81361 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
    81421 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag
    81481 ccggagatgg cgttcagacc agatccggca acattatccc acgcatggtc agcaaactga
//
LOCUS       DAZOXR010000002        64592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZOXR010000002 DAZOXR010000000
VERSION     DAZOXR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.002"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUNN_000081"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUNN_000081"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0574585.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUNN_000082"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUNN_000082"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0574586.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUNN_000083"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUNN_000083"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0574587.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUNN_000084"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUNN_000084"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR0574588.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUNN_000085"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUNN_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR0574589.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUNN_000086"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUNN_000086"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0574590.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUNN_000087"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUNN_000087"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0574591.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHUNN_000088"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHUNN_000088"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0574592.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUNN_000089"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUNN_000089"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0574593.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHUNN_000090"
     CDS             complement(12799..14139)
                     /locus_tag="ACHUNN_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0574594.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUNN_000091"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUNN_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0574595.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHUNN_000092"
     CDS             complement(15928..16377)
                     /locus_tag="ACHUNN_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0574596.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUNN_000093"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUNN_000093"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0574597.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHUNN_000094"
     CDS             complement(17177..17506)
                     /locus_tag="ACHUNN_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0574598.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUNN_000095"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUNN_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0574599.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHUNN_000096"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHUNN_000096"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0574600.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUNN_000097"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUNN_000097"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0574601.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHUNN_000098"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHUNN_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0574602.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHUNN_000099"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHUNN_000099"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0574603.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNASRMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="ACHUNN_000100"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="ACHUNN_000100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0574604.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHUNN_000101"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHUNN_000101"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0574605.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHUNN_000102"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHUNN_000102"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0574606.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="ACHUNN_000103"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="ACHUNN_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0574607.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="ACHUNN_000104"
                     /pseudo
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="ACHUNN_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="ACHUNN_000105"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="ACHUNN_000105"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR0574608.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="ACHUNN_000106"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="ACHUNN_000106"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0574609.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="ACHUNN_000107"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="ACHUNN_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0574610.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHUNN_000108"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHUNN_000108"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0574611.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="ACHUNN_000109"
     CDS             complement(34678..35073)
                     /locus_tag="ACHUNN_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0574612.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHUNN_000110"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHUNN_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0574613.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36360..36587)
                     /locus_tag="ACHUNN_000111"
     CDS             complement(36360..36587)
                     /locus_tag="ACHUNN_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0574614.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36779..37984
                     /gene="csdA"
                     /locus_tag="ACHUNN_000112"
     CDS             36779..37984
                     /gene="csdA"
                     /locus_tag="ACHUNN_000112"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0574615.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37984..38427
                     /gene="csdE"
                     /locus_tag="ACHUNN_000113"
     CDS             37984..38427
                     /gene="csdE"
                     /locus_tag="ACHUNN_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0574616.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHUNN_000114"
     CDS             complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHUNN_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0574617.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHUNN_000115"
     CDS             complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHUNN_000115"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0574618.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40667..40743
                     /locus_tag="ACHUNN_000116"
     tRNA            40667..40743
                     /locus_tag="ACHUNN_000116"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40701..40703,aa:Met,seq:cat)
     gene            complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHUNN_000117"
     CDS             complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHUNN_000117"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0574619.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42303..43634
                     /gene="argA"
                     /locus_tag="ACHUNN_000118"
     CDS             42303..43634
                     /gene="argA"
                     /locus_tag="ACHUNN_000118"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0574620.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHUNN_000119"
     CDS             complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHUNN_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0574621.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHUNN_000120"
     CDS             complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHUNN_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0574622.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49057..51945)
                     /gene="ptrA"
                     /locus_tag="ACHUNN_000121"
     CDS             complement(49057..51945)
                     /gene="ptrA"
                     /locus_tag="ACHUNN_000121"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0574623.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52121..55489)
                     /gene="recC"
                     /locus_tag="ACHUNN_000122"
     CDS             complement(52121..55489)
                     /gene="recC"
                     /locus_tag="ACHUNN_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0574624.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55502..55825)
                     /locus_tag="ACHUNN_000123"
     CDS             complement(55502..55825)
                     /locus_tag="ACHUNN_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0574625.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55810..56217)
                     /locus_tag="ACHUNN_000124"
     CDS             complement(55810..56217)
                     /locus_tag="ACHUNN_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0574626.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56214..56777)
                     /locus_tag="ACHUNN_000125"
     CDS             complement(56214..56777)
                     /locus_tag="ACHUNN_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0574627.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56768..57238)
                     /locus_tag="ACHUNN_000126"
     CDS             complement(56768..57238)
                     /locus_tag="ACHUNN_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0574628.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57423..58217)
                     /gene="thyA"
                     /locus_tag="ACHUNN_000127"
     CDS             complement(57423..58217)
                     /gene="thyA"
                     /locus_tag="ACHUNN_000127"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0574629.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58224..59099)
                     /gene="lgt"
                     /locus_tag="ACHUNN_000128"
     CDS             complement(58224..59099)
                     /gene="lgt"
                     /locus_tag="ACHUNN_000128"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0574630.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59250..61496)
                     /gene="ptsP"
                     /locus_tag="ACHUNN_000129"
     CDS             complement(59250..61496)
                     /gene="ptsP"
                     /locus_tag="ACHUNN_000129"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0574631.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61509..62039)
                     /gene="rppH"
                     /locus_tag="ACHUNN_000130"
     CDS             complement(61509..62039)
                     /gene="rppH"
                     /locus_tag="ACHUNN_000130"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0574632.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(62392..62538)
                     /gene="ygdT"
                     /locus_tag="ACHUNN_000131"
     CDS             complement(62392..62538)
                     /gene="ygdT"
                     /locus_tag="ACHUNN_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0574633.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62724..63413
                     /gene="mutH"
                     /locus_tag="ACHUNN_000132"
     CDS             62724..63413
                     /gene="mutH"
                     /locus_tag="ACHUNN_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0574634.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63482..64195
                     /locus_tag="ACHUNN_000133"
     CDS             63482..64195
                     /locus_tag="ACHUNN_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0574635.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64333..64551
                     /gene="ygdR"
                     /locus_tag="ACHUNN_000134"
     CDS             64333..64551
                     /gene="ygdR"
                     /locus_tag="ACHUNN_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0574636.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15652 a        17368 c        15824 g        15748 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt cagatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacacgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgcctcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagt gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccgga ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgccacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 acttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attccaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagctaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgggaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgtgcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc gctttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtta aattaattta acaattagat cacactgtgt
    36061 aacctattag tttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa
    36121 atacctatag tggcggcact tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc
    36181 tggtgaactt ttggcttacg gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga
    36241 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    36301 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    36361 tactgatcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    36421 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    36481 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    36541 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    36601 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    36661 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    36721 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    36781 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    36841 ctatctcgat agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    36901 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    36961 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    37021 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    37081 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    37141 taacctcgtc ccctggctga tggtcgccca acaaactgga gccaaagtgg tgaaattgcc
    37201 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    37261 tcggattctg gcgttgggtc agatgtcgaa cgttactggc ggttgcccgg atctggcgcg
    37321 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    37381 ggtgcatttc cccgcagatg ttcagcaact agatatcgat ttctatgctt tctctggtca
    37441 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    37501 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggcttcac
    37561 gactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtggctg gtgtcatagg
    37621 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggcag aaagttggag
    37681 tcgcagcctg gcgacgctgg cggaagatgc gctggcaaaa cgtcctggct tccgctcatt
    37741 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    37801 ggtgacgctg ctggcggagt acggtattgc cctgcgggcc gggcaacatt gcgctcagcc
    37861 gctactggca gaattaggcg taaccggcac actgcgcgcc tcttttgcgc catataatac
    37921 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    37981 ttaatgacga acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    38041 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    38101 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    38161 ggatgcgaaa accgcgtctg gctgggatat accgtgactg aaaacggtaa aatgcacttc
    38221 tttggcgata gcgaagggcg cattgtgcgc ggcctgctgg cggtgctgtt gactgccgtt
    38281 gaggggaaaa ccgccgccga attgcaggca cagtcgccgc tggcattgtt tgatgagctg
    38341 ggattgcgtg cgcaacttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    38401 attatcgctg cgacgaagca ggtttaagca tagaaccggg tcggataaga cgttcgcgtc
    38461 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    38521 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    38581 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac agtaccgtct
    38641 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat cgacgccgag cttaccttta
    38701 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    38761 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    38821 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    38881 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aacccacgct catatactgc
    38941 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    39001 tggcgaatac gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    39061 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    39121 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    39181 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    39241 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    39301 aaagggtgag gttatccctc accctgtcgt atccgtaaaa acggtataca gaataacaca
    39361 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39421 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    39481 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    39541 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    39601 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    39661 ccctttcacc ggtgcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39721 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39781 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39841 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39901 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39961 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    40021 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    40081 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    40141 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    40201 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    40261 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40321 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40381 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40441 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40501 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40561 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40621 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggaggcg gggtggagca
    40681 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40741 ccaattattg aacaccctaa cgggtgtttt tttgtttcta ctctcccata aaaaaagcgc
    40801 cattcagcgc ctttttatca tccccttctc gccagcgttg ccccatcggc aaaatacgct
    40861 ttaatccccg caagaataga ctccgcaact tcctgctgga aagtcgccgt tttcagttta
    40921 cgctcttcct caacgttact gataaacgcc gtttcgacca gaatggaggg aatatctggt
    40981 gcctttagta cggcaaaccc ggcctgttca acttgatttt tatgcagctt gttgatttta
    41041 ccgagcttat tcagcactgc tttaccaaac ttaaggctgt cagcaatggt cagcgactgc
    41101 accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac gccaccaatc
    41161 aagtccgagg cgttctgggt ttgtgccaga tattttgccg cagtactggt tgcacctttg
    41221 gttgagagcg caaacaccga ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg
    41281 atagagacaa acaggtcagc acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga
    41341 atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttt ctcgatcaga
    41401 gagcgcaaac ggcgagctat ttgcaatacc acatcttttt cgcgcgtttt gtatttcccc
    41461 accgcaccgg agtcttcgcc accgtggcca gggtcaagca taatgacaat cggacgatcg
    41521 cgccctgctt tacctggttg tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg
    41581 cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg tgcattggcc
    41641 ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag ggcaaacagc
    41701 tgcggtttta cgttttgctt taattcaaaa accatccgta cggtttgcgg gtcaaattgc
    41761 ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc catccccttg
    41821 agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttcagg gttactcaac
    41881 gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt gtagctggac
    41941 gcaggccaga cgcgcaccgc cacgacctgg ctaaccgcag ccaggctgac ctgacttacg
    42001 ctcaatagcc acatagcgcc cgcgccttgc agtaaacgac gacggctgat tgcagtgttg
    42061 gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag taaacgacta
    42121 atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac ggtaaacatt
    42181 ctttttatat tcacggcatt actgataaaa aagtcgctct cgcataaaat ttacacttgc
    42241 accctgcgaa aaaacagaat aaaaatacaa taatttcgaa taatcatgca aaggggtgtg
    42301 ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg gttccctata
    42361 tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc attgagcatg
    42421 agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc atccgtctgg
    42481 tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat caccacgaac
    42541 cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg gtgaagcagg
    42601 ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc aataacacgc
    42661 cgctgcaggg cgcgcatatc aacgtcgtca gtggcaattt tattattgcc cagccgctgg
    42721 gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt gatgaagacg
    42781 cgatccgtcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc gctgtttcag
    42841 tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaactg gccatcaaac
    42901 tgaaagctga gaagatgatt ggtttttgct cttcacaggg cgtcactaat gacgacggtg
    42961 atattgtctc agaacttttc cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga
    43021 aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca
    43081 gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt
    43141 tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc
    43201 gcgcaacaat caacgatatt ggcggtattc tggagttgat tcgcccgctg gagcagcaag
    43261 gtattctggt gcgccgttct cgcgagcagc tggagatgga aatcgacaaa ttcaccatta
    43321 ttcagctcga taacacgact attgcctgcg ccgcgctcta tccgttcccg gaagagaaga
    43381 ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagttcatca cggggcgagg
    43441 ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc
    43501 tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccggtg gatattgatt
    43561 tactgcccga gagcaaaaag cagttgtaca actaccagcg taaatccaaa gtgttgatgg
    43621 cggatttagg gtaagcgatg aaaatcgccg gatgcggcag gcgtaacact cgtgcgtcgc
    43681 atccggcaat tacgtttatc cccgcgaact aaacaacgcc gccagaccac tgcgccgctc
    43741 agtacgagtg gcgattgccg cacttaatat acgctcatcg gcatacagcg acagacggcg
    43801 acgcgcgcgg gtcaccgcgg tgtataccag ctctcgcgtt actatcggcg tgcgttggct
    43861 cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc
    43921 ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat tgccgtccgg
    43981 catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga tatcgccatt
    44041 aaacaaccca agcgcgctgt cattacgggc aatcatcacc ggtcgacctt cgtaccaacg
    44101 agagtgcgaa tgacgatgaa ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt
    44161 cagtccagcc acgccaaatg gcccttcccg cagggcgcac aaaagttggt actcattgaa
    44221 cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat aacgtccata
    44281 acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta aaagccgttt
    44341 ttcaatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat caccacggtt
    44401 gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc ttttttgcag
    44461 caggcagaga ctgtcgcgca aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt
    44521 tcccgtcagg cggcttagct gcccggcacg ctcggcggta aagccggcat tggcataagc
    44581 gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac gatcgccgag
    44641 aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca taggcagatc
    44701 gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac cggcatgatg
    44761 acgtaaacgc tggctacccg gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc
    44821 cggaatgcgt ctcttttgtt catcggtcag cggtaactgt cgcaaagcct tgccgagaga
    44881 ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac ggcagcgttc
    44941 gccgtcggcc atttgaatta acgctgccag caacttcgcc acggtggtcg ttttaccggt
    45001 gcctgggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgctg ccgccacttt
    45061 ttgccagtta atttcatcgc ttactggaaa aagtttgtcc agggtttgcg ccagtagagc
    45121 ttcatcaacc tcaatggcat gattcacctc attgaagaag cgcgctaccg tccgctcgtt
    45181 acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg tgggttcatc
    45241 tcccctgctg accgcttgag aagccagcaa ggattcttcc caattttgta gctcaccgat
    45301 ttcactgaca caggtcgcca acagcggatg cgacgcctcg ttattttcca gtcgtgaaag
    45361 cggcaaacag acgtgtccct ctccggcatc atgacttaac agtgccgccg cgagggtgac
    45421 ggcaggatgt tcatctcccg ccacggtcag ggcaaattgc acatccagcg ggcgtagctg
    45481 tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc ctccagggtc
    45541 ataccggcaa acatctcatc catcagggca atcaacccgg cgttgggtcg ggttgtgtaa
    45601 atcccttgtt gcggatgttc tttatcaacg ccacgcagga acagatagat aacgccgcca
    45661 aagtgacgat catagtcgta atcagcaatg cgatggcgca gatagcgatg cagcgccagg
    45721 gtataaagct gatattgcag atcatagcgg tgtgcctgca tcgctgctgc catagcctgt
    45781 tgggtgtaag ccgaactgtc ttcacccagc cagttggatt tatagtcgag caggtaataa
    45841 cgcccttcgt ggcggaatac caggtcgata aagcctttta acatgccgcg tacctgcatg
    45901 aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag tgcatcaagc
    45961 tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac ctgtttatcg
    46021 cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg gaggacagcc
    46081 gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag ttccagtttt
    46141 tcctgtaccc agttcgggtc aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc
    46201 aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt cggttcttca
    46261 acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc ctgagcgata
    46321 ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc gccgggcaat
    46381 cgttgtagtg tcctcgcatt cagttctgca gttaaagcat cattaacctg ccagggctgg
    46441 ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc ttcaatacag
    46501 gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc gagcgcactt
    46561 tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg
    46621 agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc
    46681 agacgttcga cctccgcgag gtcgacgctt tctggcgcag cattaagatc caaaactgcc
    46741 tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat
    46801 ggcaaccaga ccagcggata ttccagccct ttcgatttat ggatcgtgac aatctgcacc
    46861 agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc tggctcgagg
    46921 atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt tccggcttct
    46981 tgaagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc
    47041 agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcagcatcgg cataacgcca
    47101 cgtttgcgcc agatttgccg ataaccatcg aactcttcga ctaccgcatc ccacgcatgt
    47161 tcgtcattat tcagcgtttc aatatccagc gcattcagcc ccatcattga cgttgccagc
    47221 gcactgcgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacag ccaaagcatt
    47281 tcctgcgctt ccagagtttc aaaaacacta tcgcggttcg aaaggtaaac ggaagggatt
    47341 tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct ggcggctgcg caccagcacg
    47401 ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc
    47461 cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc catggtactt
    47521 tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat cgcaggctgc
    47581 gtttcacctt taaagacaaa acgtaacgcc tgatttttcc cggctgattt cactggaata
    47641 aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt gttcacactg
    47701 ttcaccattc caggtgcgga gcgccagttg gtgtctaaag tgtagtgggc gtgaacttca
    47761 ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat ggcctgcttc
    47821 gggtcgccaa tcagcaacaa tgcggtttct ggctgatggt gccagatacg gcgaaaaatt
    47881 cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta
    47941 cggatcgccg ctgccaacac ctcgccgctt tcgctacgca gcgcggaatc gagccgactt
    48001 aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg
    48061 atctcagcca atgcgcgggt gatcaccaga tcacggatcg acaatggttc tgcaagcagt
    48121 tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct
    48181 tctaagaaac gctgggagaa tttttccagc gactccggca actgataact gtttgtctct
    48241 tcttctgccc aggcgctgat cttgtcgatc catttagcct gattgctacg gttaaacttg
    48301 cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc gtcgcgccac
    48361 tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt gacgggaagc tagcgtttca
    48421 tcatcgggcg gcggtgcttt gataaccggc gcttcgcctt gcagataacg attaatatcg
    48481 cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggctat ttcacgcggc
    48541 agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag cagagactca
    48601 tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag gttgagcatg
    48661 cgctggcaaa agccgtgaat agtaaagact gccgcttcat ccatctgccg ttcggctaac
    48721 aacaaccact gcgcggcttg cgctttatcg tcgacctctt ccagcaggtg ttcgtacaat
    48781 ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt gctacggata
    48841 cgaccgcgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg
    48901 gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg caaataaagc
    48961 gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt
    49021 aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt cactcatcag
    49081 gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt caggatgcac
    49141 atattcggct ttcccgtttt ggctgccgga aatctgcgac aggatagcca tgccttgcgg
    49201 ctcgactacc gcctgatgga agaaatcggc cagtttttgc ggcgtcagca gtttaatctg
    49261 ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat ctttacttaa
    49321 cttcgatgct tcttcgccga gcgtttgcgg tgcctgcagc atctgggtaa ttaccgcctg
    49381 ctggatttgc gcaaactcat ctggcttcat cgctcgcaat tttgcctctg cggttgggaa
    49441 aaacgccttg taacgctccc acaagaatga aggctgttta tcattgcttt gcaaaaggaa
    49501 gcccatgccc cactgacgcc ccacgctcat tggaaacgca aacacggcat agcccaattg
    49561 ttcttcggta cgcaactgat tgtagaacca cggctgtacg atctgcccca acagagagct
    49621 ataggctgag ctggtgtatt catcgtagcc agtcggtaca aataccgctg ccagtgcgga
    49681 gtcggtgctg ttaccggctt tttcaaagat gacggattgt tttttatcga ccactacatc
    49741 tttgtttcga caccactctg aaccatcagc gcccaactgt ttttgcacat cgcgtgccag
    49801 cgttgttgcc tgggcctcgg tcatgttgcc gataaccata aactctggtc gagcccctga
    49861 ttttaaggcg tcgcgataag ccagcacctc tttcaacgta atggagggca aaattttacg
    49921 ccgttcatct cgcgagaagt acggcacttg cgagagcatc tgcgcgggca taatcgcctg
    49981 ctcaaacgct ttaccctttt ctgcggaatc catcatctgg ttataccagg acttcgcctg
    50041 ctcaagctga tcttccgtag cggtatagct aaagtacccc tcgagcaatg cctggaacag
    50101 ctgcggcaga cgctgggtgt aaccattagc attaaccata aggccgttgt tagcgttggt
    50161 ggaaaaactt atgccaccaa ccgacgcctg gttgcttaac tgatcaagcg ccagccctgc
    50221 gagataatca ttgagcgcaa acatcacctg attgcgggcg ctgtccatgg ctttcggatt
    50281 acgcaaaatc aggctgacat cagctttggg ttcgctggaa aaataacggc ttggcgcata
    50341 caccacgcgc agattcgact catcaacaat cagctctgga tggtcatatt tcttctctga
    50401 cttaatcagc gagaaatcgt caggaatata ggggttaagc tccggtaagg agagcgcaat
    50461 attggcagct ttttgctgcc agtcagcgaa agtttgttcg ctgattttat cgacctgata
    50521 cggcgcatcg acaaaataag ccgttttatt gtgcggctct ttcgggctga tataccagat
    50581 acgcgcattc tgcggcgtca tcatcgccag acgttctttt actgctttag catcgtaccg
    50641 atcggcaata ttgactgcat ccagcgtatg ctcaaccgga acgcgaatca tggtatctgc
    50701 cagccattcg acgtaatcca tatcacgggt gatcgacgga taacggaagt cgatatccag
    50761 cacattcgcc agttcatcga agtattgttt atcaatgcct ttttcacgta acagattgag
    50821 atagctaaaa attgccgcca caacctgatc gcgattagcc aggcctttat cggttaaaga
    50881 cgcagagatc gctaatacgc cgctgttgcc gttgacgata ggatcggagt tggcgctaat
    50941 gccctcaact aatccctgct tttgcagcca gtcggaaagt gtacctgggc tgcgattacc
    51001 aatcagatag gtaatcaatt catccgtttt actacggaac ttcgctgagt tgttatcgat
    51061 gcgaaactca acgcgcagaa ctttacgcgg cattgccggg acgtaatgaa tgataatgcc
    51121 cttttgcgcg tcggtgacta ccggcacggt gatttccggt tttttgctct ctttgttcgg
    51181 cacgcgacca aaggtgtccg ccgccatttt tgccaactcc ggcagcggtt tattgctgta
    51241 aataaccgcc ttcatcagat tggcggaata gtacttctcg tggaaatctt tcagcgcctg
    51301 ctgcaccgga ttaccaggtt tatcgcttaa agtttcgagg ttaccaccag aaaactttga
    51361 accggggtgt gccgggttaa tggtttctgc gctgacctgt gccatgcgca tcccgtcacg
    51421 cgtacgcgcc atggttaatt cagcgttcac cgcattacgc tcacgttcgg catatttctt
    51481 gtcgagcaaa ggttcagcaa tagcatcggc caggcggtct accgcaccag gcaaggcgtc
    51541 gttctcaact tccagataga aagccgtgcg atacggcgca gtgctggcat tgtgactacc
    51601 gccgtgcatt ttgagatatt cggccagact gtcagcctgc gggtactttt tcgaccccat
    51661 cagactcata tgttcaaggt aatgtgccag cccctggtac gcctcgggat cttccagcga
    51721 cccaacgggc accaccagcg ccgagagcga tttaactgcc tgcggatcag aaaccagcaa
    51781 gaccaccata ccgttatcca gacgtatagc ctgatactgg cggttatctt tatcactttt
    51841 acggatggtt tcctgaatcg gctgccatcc cgtttctgcc tgacttaagg gtgcccaaag
    51901 ggcaactaac aacaataatg ctttgaacca ggtgctgcgg ggcattctcg gacctcataa
    51961 tcaacttaat tttctgtcca gattcaacac gttaacgcaa atcatctgcc agaatttaat
    52021 cttgtgctgc acgagtcagc ctatgtttat ataaccatca gtccgtgact ggtgcgcatc
    52081 ataaagtaag cggatagatt gcgcaatttt tatacagcac tcatgactga ttaaagcgaa
    52141 acagcggtaa caggaaacgt tgcgactgtt caacgatggc ctccattgtc tctggtgtta
    52201 attgccgcca gagcctttga taccagatat catcaccttc gccacgcacc atcatgttac
    52261 cttcgtaagc ctgaaggaat ttcgtacggg ctttttgcaa cgtggaatcg tcatccagca
    52321 tggcatcgtt ttgcgcgtca taacaggttt ttagccacgc gccgccactt tcaggtaaca
    52381 ccagcaatgg cgcggacatc ccttcacgat acccctcaat cagttgtgag aggtaatgca
    52441 aagcctgttc ggctgcaagc ggcggaaaac gccactcgcc gtctttgcgt agaaaaaggc
    52501 gactttcacc attaccaccg ctggcacagt agacaaggtg ttccagccaa agttgcattc
    52561 cctgcgccac acttaataaa gagggacgcc agcgcaacag gccatccggc tgcacctgcg
    52621 gcaaccagcc agttatctgc acaccgttgc aggcgagatc aatttccata ctctgccccg
    52681 gctggcgaca ggcaatgact ctgtcggcaa gctgctgcat ctcctggcac tgagtttccc
    52741 agaaaatttc accaaaagcg ccatacggta aatcccctgc cgcccggaag cgacggaaca
    52801 agcgttcggc atcatcctgc tcaaccagtg cattcaataa ctgctgattg atttgataac
    52861 ggctaagccc ttccagaata aatggctcgg tgtcggggat ttcgctgtct tcagtacgga
    52921 agttcacctg caaacgcatc tggaagaacg cccgcaccgg atgtgcccag aatcgttgta
    52981 gcgtttccag cggcacggtt tccggtaagg taaacggcag cggctgaaca aattcagaat
    53041 gtgctttacc agcctggctg gccgcaggta gccattcacg agcatagctt tgtcgttcgc
    53101 ctggctgata gttttgtgga tcaaacggca ttcgggtatg gaggcaagta agatgcgctt
    53161 ttacccttgc ctcgctttca tcacagttga gcgcttcatc gcccggtaga taatgacttt
    53221 gcccgatgta gtcgatcagt tcctgcacca gtaccgacgg gaaacgttca ctgttatcct
    53281 gaatggaacg cccgatatag ctgatataga gtttttgctg cgcggaaatt aacgcttcca
    53341 ggaacagata gcggtcgtca tcgcgacggc tacggtcgcc acgcttcggt ttctggctca
    53401 tcaggtcaaa gcccaatggc gcaagctgac gtggataaac gccgtcgttc attcccagca
    53461 ggcaaaccac tttgaacgga attgaacgca ttggcatcag agtacaaatg ttaaccggtc
    53521 cggcgagaaa acgctggctg atacgttctt gatccagacg ctgtgccagt tcatcacgca
    53581 atagtgacag cggcaccgcg tcgccatact gcgcacctaa accttcggcg ataatcgcct
    53641 gccattgttg ttcgatcagc gtcatcgccg cttcggtttc cgcatccggc aggaagaagg
    53701 cgctgagcat atcgcgacaa accggcaacc actcttctag cggacgctcc tgtgccagcc
    53761 cgcgacgcca gatgtttagc tgcattagca gtgaagccag atgccccacc agttctgcaa
    53821 ttaagccgct cgattcatca taaggtagaa ccgattgcca ctcgccctgc gcgctctcca
    53881 tcgcgtagcc cagcaacata cgcgtcaggc caaatcgcca ggtgtgttgt ccggtggcgg
    53941 ggagttccag ctcgcgaacg ttgtcgtcat ctatccccca acgaatgccg gattcgttga
    54001 cccactggcg taaataacgc agcccttctt cggtgatgtc aaaccgcgcc gccagcaccg
    54061 gcacatccag taatgccagc acgtcttccg acacaaagcg gctgtctggc aacgataaca
    54121 ggctgataaa cgcttccagt accggatgcg actgccgcgc ccgacggtcg gaaatggcgt
    54181 aaggcaggta acgatccgca ggtgcgctac caaacacagc ccgaataaac ggactgtagc
    54241 tgtcgatatc agccaccatc acgatgatgt cgcgcggagt aagtgtcggg tcttcttcca
    54301 gcatcgccag caggcgatcg tgcaaaactt caacttcacg ctgcgggcta tggcaaacgt
    54361 ggaaggtgat actgctatcc agtggatcaa gcgggcgttt gttatcgcta cgggaaaact
    54421 cttcgatgtt cacaccagca acggcacggt tttccagttc cagaatgtca gactgaatgt
    54481 tatgcagcag gttatctggc gtgacatcga caaaggcgtc cagctcctgg ctgctctcca
    54541 ggtcagaaag gagataaatg tagtcgcgcc caagcttacc ccatgaagcc agcagcgggt
    54601 tgccgacatc ctgttcacca tcgctgttaa agagctgccc ggcattttcg ctatcacgaa
    54661 atagcggcaa ttcgcgatct tcaaaactgt gtcgacgctg acgggtcagc agtttcgcca
    54721 gataagcagg atctttaata tcgccccagt aataacggca ggggttggta aacaggagat
    54781 ggatttcaat atgtttaccc agcgcctgta gcgcctggag ataaacaggc ggtaacgcgg
    54841 aaataccgca tataaagacg cgcgaaggta accccggcgg gcaggtcgtc gcggactcca
    54901 gcgtttcgat aaagcgctga tagagattgg cgcggtgcca gcgcggttgc ccgagttgat
    54961 gggtatattc caccagcgcc ttccacaacg gggcttgcca ggcctgtgct tctcccagcc
    55021 cttcaaccaa atgtcctgtt tcccactgtg ccagccagtc cggacgatag accagatact
    55081 ggtcaaacag gtccgccgct tttgaggaaa gctggaacag ttttcgcttg tcgctatcgt
    55141 cagtcagata atgccgcaac agggtaaagt cttcgcgctc cagcaattgc ggcagcagag
    55201 tcatcagttt ccagctcatg ctctgtttgt taaaggcgct ctctttgggg atttccggta
    55261 acacccggac gaacatatcc cagataaagc tcgctggcag cggaaaatca atgtttgccg
    55321 caatactaaa cttttgcgac agggtcattt gcagccactg cgccataccg gtactttgca
    55381 ccagaatcat ctctggttcg aaaggatcgt ccagccgttc gcgttcgaca ataaactcca
    55441 tcaacgcttc cagcacgtcc agacgattgg aatggtagac ccttaacata gcggctcctg
    55501 actactgacg attcgggcaa tgcaggcggg tcatctcgcc ttctctgccc ccgggtgaaa
    55561 ctagcgtaac gctgatgctg acacatcccg cctgcgatgt ctgcattcgg ttgacctgcc
    55621 agttggcagg tggcgaaatc gcgcgcagtt gcgtttgctg ccaaccatgc cgccagagct
    55681 gttggtactg gtttctgctg gcgagactgt tcattaatgt tcgctggata cccgataacg
    55741 cagtgacaat catcaccatc agcaccatcg caaccattac ctccggcagg ctaaagcctt
    55801 gttgattctt cagggaagct gacataacgc cctctctttc agcggacaaa aatcgctcca
    55861 gccgcgtggc gaaaaaacaa tgtttccatc aatgacttcg cctgttcgcc acaacgaaac
    55921 gccttcataa ccggcaacca ataaggcttc attatctgcc agtaaacgca aacaaacccg
    55981 ggcatcggtt ccagcgtact gcgagcactg aactgcgggc tgcgtctgcc aggagtgcat
    56041 tttcccccac gccagcgccg actgaacgat ggcctggcgg cgcaatgact gactttctat
    56101 gctcacgcga gaggcaaaac tgcgatcctg ctgactcatt ccttgcaata gcaagctacc
    56161 caaaaccagc agcatcagga ccagagccag tgacgaaaca cctttttcgc ggttcacagg
    56221 ttgaatcctg tcacgctata gctggcatcc accacggttt gcggttcaga cttactggca
    56281 gcacgcatat taaccgtcaa caccggcgag aagccgctga catcctgacg tacgacctga
    56341 aaagtgtcga taatgatggc atccggatta gtgactttat cccagccctt accttcacag
    56401 gatgtcgcac cgcgtagcgt ttccagcaca tgctccttca gacgaaatcc aatctggtca
    56461 gactctttta ccggttcgcg atcccagata ccgttactgt tcgcatccca ctgcacaatg
    56521 atacagtcac cctgtccgac aatttccagc ccttcgccgg tacagctgcc atgacaataa
    56581 cccgccctct ggagatgctt cgcgacggta aatacccgca gccagatttc atcttccagc
    56641 gccagcttac gggtgctcgt taaactttca cgctgtaacg caggcagaaa gcgtgcagcc
    56701 cccagcaaca atacgctact gatcgccata gcaatcaaca cttccagcag agaaaaacct
    56761 tgctctttta caggcatcct tctgtttctc cttgctgaca aagccggagt cttccccacg
    56821 gcgaaaccac cagccaccac tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg
    56881 cggtattgcg caggccaaag aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg
    56941 gccagcgtgg cacaaagacc aatggtgaac tgccatgaca ggtattggcc ccagcagcgg
    57001 aactcacaag gcaccataac gtcccctccc tgataacgct gatactgtgg tcgcggttat
    57061 gccagttggc atcttcacgt aaatagagca aatagtcccg cgcctggctg gcggtttgcc
    57121 atagccgttg cgactgctgc cagtattgcc agccatagag tccacttgcg cttagcatga
    57181 ccaaaatcag catcgcgacc agcgtttcaa tcagcgtata accacgttgt gttttcatgc
    57241 cggcagtatg gagcgaggag aaaaaaagac gagggccagt ttctatttct tcggcgcatc
    57301 ttccggacta tttacgccgt tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa
    57361 tttaacggag ggtaaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt
    57421 aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtagccttc aatctcaaag
    57481 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
    57541 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
    57601 gtatcgccac cggtccagac aaaatcaccc acttccagat cgcactgctg cgccatcata
    57661 tgcaccagta acgcgtagct ggcaatgttg aacggcaggc cgaggaagac gtcacaggag
    57721 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg
    57781 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
    57841 cgggaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
    57901 ccatctggcg ttggccaggc gcgccactgt ttaccataca ctggtccgag gtcgccgttt
    57961 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
    58021 tcgccctgca gaaaccacag cagttcgtgg atgatggaac gcaggtggca acgtttagtt
    58081 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
    58141 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
    58201 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
    58261 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg
    58321 atgtactgca cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
    58381 atgcgaaacg cgccgtagcc aatcaggaac aagccagaga cagctcccat tgggcgcggt
    58441 ttacgaatat acaggttgag gataataaac agcaccacac cttccagcag cagctcgtaa
    58501 agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg
    58561 ttggtttgca gcagcaaaat atcttctgta cgggagcctg ggaacagcat ggcaaacggg
    58621 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
    58681 ccaagaccaa acggaatgag tggtgcgata aaatcagaaa cctggaagaa ggaacgttta
    58741 gtacggcggg caaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaagac
    58801 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaaattg
    58861 tagaacagaa cataaccaat acgtcccccg aggaagacgc cgaggaagcc cgcatagagt
    58921 aagttttcaa cttcattttt ggtccagccg ctgcccggac gattcgcccg tcgtgttgcc
    58981 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
    59041 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
    59101 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
    59161 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
    59221 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
    59281 ccgcgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc
    59341 agactacgct gcgcaagatt ttctgcttcg gcataatcaa tgcgccgcag caggtatttt
    59401 acccgcgcta cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt
    59461 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat tccatgtatt
    59521 tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcataa
    59581 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
    59641 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
    59701 ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
    59761 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg
    59821 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
    59881 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
    59941 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
    60001 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
    60061 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
    60121 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
    60181 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
    60241 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc
    60301 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
    60361 agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg
    60421 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
    60481 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
    60541 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
    60601 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
    60661 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
    60721 aacaattcgc gacgcagccg ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc
    60781 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
    60841 gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc
    60901 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
    60961 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa caaggcagtc
    61021 aactgcgact gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
    61081 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
    61141 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
    61201 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
    61261 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
    61321 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
    61381 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
    61441 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
    61501 ggcgtgactt aacctctttt acgtcgataa gcagatgtgt tttgtggttt tggcgtattt
    61561 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
    61621 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
    61681 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgctcaccag ctgcaagaga
    61741 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
    61801 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
    61861 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
    61921 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
    61981 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
    62041 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca agaagacggt
    62101 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
    62161 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
    62221 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
    62281 tagacatttt aaaatattca ttattagtca atgcattaca tgtaatttga gtaatgaaaa
    62341 agagtaattc gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat
    62401 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
    62461 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
    62521 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
    62581 cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat aaatcagaga
    62641 attgaacaac acatgtggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg
    62701 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actcctctct cctcccgaaa
    62761 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
    62821 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
    62881 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
    62941 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
    63001 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccatg
    63061 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggtgagcgc agcatcccgc
    63121 tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag gaagaccgac
    63181 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
    63241 tcaccgctcg tcacggggag tatttacaga tacgaccgaa agcagcgaat gcgaaagcgc
    63301 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
    63361 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
    63421 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
    63481 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
    63541 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
    63601 ccccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
    63661 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
    63721 attcagtcag gaaatttccg cccgtgattt gattctgctt ctgggtggct tgttccttat
    63781 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
    63841 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatcttcag
    63901 cctcgactcg gtgattaccg ctgtgggtct gtcagatcac ctgtttatta tgatggccgc
    63961 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
    64021 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtcg gctttaccct
    64081 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
    64141 ctctattgcg gttgaaagcc tcaacctgat tcgcaacaaa aagaacccgc tctgatactc
    64201 cgtacgctct cctgcgggag ggcgtactca ctcctgttac cctcacagtt taagattttc
    64261 ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg tgggccaaat
    64321 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
    64381 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
    64441 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
    64501 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
    64561 tcagcatccg gctgtaatgc cgatcagtta ag
//
LOCUS       DAZOXR010000003        62996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZOXR010000003 DAZOXR010000000
VERSION     DAZOXR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.003"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..413)
                     /locus_tag="ACHUNN_000135"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="ACHUNN_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHUNN_000136"
     CDS             complement(519..3080)
                     /gene="mutS"
                     /locus_tag="ACHUNN_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0574637.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3156..3290
                     /locus_tag="ACHUNN_000137"
     CDS             3156..3290
                     /locus_tag="ACHUNN_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0574638.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3367..3720
                     /locus_tag="ACHUNN_000138"
     CDS             3367..3720
                     /locus_tag="ACHUNN_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0574639.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHUNN_000139"
                     /pseudo
     CDS             complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="ACHUNN_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHUNN_000140"
     CDS             complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="ACHUNN_000140"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0574640.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHUNN_000141"
     CDS             complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="ACHUNN_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0574641.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHUNN_000142"
     CDS             complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="ACHUNN_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0574642.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHUNN_000143"
     CDS             complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="ACHUNN_000143"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0574643.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHUNN_000144"
     CDS             complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="ACHUNN_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0574644.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9737..10198
                     /gene="hycA"
                     /locus_tag="ACHUNN_000145"
     CDS             9737..10198
                     /gene="hycA"
                     /locus_tag="ACHUNN_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0574645.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10323..10934
                     /gene="hycB"
                     /locus_tag="ACHUNN_000146"
     CDS             10323..10934
                     /gene="hycB"
                     /locus_tag="ACHUNN_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0574646.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10931..12757
                     /gene="hycC"
                     /locus_tag="ACHUNN_000147"
     CDS             10931..12757
                     /gene="hycC"
                     /locus_tag="ACHUNN_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR0574647.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12760..13683
                     /gene="hycD"
                     /locus_tag="ACHUNN_000148"
     CDS             12760..13683
                     /gene="hycD"
                     /locus_tag="ACHUNN_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0574648.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13701..15398
                     /gene="hycE"
                     /locus_tag="ACHUNN_000149"
     CDS             13701..15398
                     /gene="hycE"
                     /locus_tag="ACHUNN_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0574649.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15408..15950
                     /gene="hycF"
                     /locus_tag="ACHUNN_000150"
     CDS             15408..15950
                     /gene="hycF"
                     /locus_tag="ACHUNN_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR0574650.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15950..16717
                     /gene="hycG"
                     /locus_tag="ACHUNN_000151"
     CDS             15950..16717
                     /gene="hycG"
                     /locus_tag="ACHUNN_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR0574651.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16714..17124
                     /gene="hycH"
                     /locus_tag="ACHUNN_000152"
     CDS             16714..17124
                     /gene="hycH"
                     /locus_tag="ACHUNN_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR0574652.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17117..17587
                     /gene="hycI"
                     /locus_tag="ACHUNN_000153"
     CDS             17117..17587
                     /gene="hycI"
                     /locus_tag="ACHUNN_000153"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR0574653.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17612..18385
                     /locus_tag="ACHUNN_000154"
     CDS             17612..18385
                     /locus_tag="ACHUNN_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0574654.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18547..18858
                     /locus_tag="ACHUNN_000155"
     CDS             18547..18858
                     /locus_tag="ACHUNN_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR0574655.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18855..19274
                     /locus_tag="ACHUNN_000156"
     CDS             18855..19274
                     /locus_tag="ACHUNN_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0574656.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHUNN_000157"
     CDS             complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="ACHUNN_000157"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR0574657.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHUNN_000158"
     CDS             complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="ACHUNN_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR0574658.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22538..23548
                     /gene="ascG"
                     /locus_tag="ACHUNN_000159"
     CDS             22538..23548
                     /gene="ascG"
                     /locus_tag="ACHUNN_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0574659.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23696..24223
                     /gene="hydN"
                     /locus_tag="ACHUNN_000160"
     CDS             23696..24223
                     /gene="hydN"
                     /locus_tag="ACHUNN_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0574660.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24376..26628
                     /gene="hypF"
                     /locus_tag="ACHUNN_000161"
     CDS             24376..26628
                     /gene="hypF"
                     /locus_tag="ACHUNN_000161"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0574661.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26756..27889)
                     /gene="norW"
                     /locus_tag="ACHUNN_000162"
     CDS             complement(26756..27889)
                     /gene="norW"
                     /locus_tag="ACHUNN_000162"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0574662.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27886..29325)
                     /gene="norV"
                     /locus_tag="ACHUNN_000163"
     CDS             complement(27886..29325)
                     /gene="norV"
                     /locus_tag="ACHUNN_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0574663.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29512..31026
                     /gene="norR"
                     /locus_tag="ACHUNN_000164"
     CDS             29512..31026
                     /gene="norR"
                     /locus_tag="ACHUNN_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0574664.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="ACHUNN_000165"
     CDS             complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="ACHUNN_000165"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0574665.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="ACHUNN_000166"
                     /gene_synonym="gutR"
     CDS             complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="ACHUNN_000166"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0574666.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="ACHUNN_000167"
     CDS             complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="ACHUNN_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0574667.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="ACHUNN_000168"
                     /pseudo
     CDS             complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="ACHUNN_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="ACHUNN_000169"
                     /gene_synonym="gutB"
     CDS             complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="ACHUNN_000169"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0574668.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="ACHUNN_000170"
                     /gene_synonym="gutE"
     CDS             complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="ACHUNN_000170"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0574669.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="ACHUNN_000171"
                     /gene_synonym="gutA"
     CDS             complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="ACHUNN_000171"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR0574670.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFLHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36215..37300
                     /gene="mltB"
                     /locus_tag="ACHUNN_000172"
     CDS             36215..37300
                     /gene="mltB"
                     /locus_tag="ACHUNN_000172"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0574671.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37445..37942
                     /gene="pncC"
                     /locus_tag="ACHUNN_000173"
     CDS             37445..37942
                     /gene="pncC"
                     /locus_tag="ACHUNN_000173"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0574672.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38022..39083
                     /gene="recA"
                     /locus_tag="ACHUNN_000174"
     CDS             38022..39083
                     /gene="recA"
                     /locus_tag="ACHUNN_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0574673.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39311..39811
                     /gene="recX"
                     /locus_tag="ACHUNN_000175"
     CDS             39311..39811
                     /gene="recX"
                     /locus_tag="ACHUNN_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0574674.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39939..42569
                     /gene="alaS"
                     /locus_tag="ACHUNN_000176"
     CDS             39939..42569
                     /gene="alaS"
                     /locus_tag="ACHUNN_000176"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0574675.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42804..42989
                     /gene="csrA"
                     /locus_tag="ACHUNN_000177"
     CDS             42804..42989
                     /gene="csrA"
                     /locus_tag="ACHUNN_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0574676.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43305..43397
                     /locus_tag="ACHUNN_000178"
     tRNA            43305..43397
                     /locus_tag="ACHUNN_000178"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43339..43341,aa:Ser,seq:gct)
     gene            43401..43477
                     /locus_tag="ACHUNN_000179"
     tRNA            43401..43477
                     /locus_tag="ACHUNN_000179"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43435..43437,aa:Arg,seq:acg)
     gene            43541..43617
                     /locus_tag="ACHUNN_000180"
     tRNA            43541..43617
                     /locus_tag="ACHUNN_000180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43575..43577,aa:Arg,seq:acg)
     gene            43816..43892
                     /locus_tag="ACHUNN_000181"
     tRNA            43816..43892
                     /locus_tag="ACHUNN_000181"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43850..43852,aa:Arg,seq:acg)
     gene            44173..44739
                     /gene="yqaB"
                     /locus_tag="ACHUNN_000182"
     CDS             44173..44739
                     /gene="yqaB"
                     /locus_tag="ACHUNN_000182"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0574677.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44736..45164
                     /locus_tag="ACHUNN_000183"
     CDS             44736..45164
                     /locus_tag="ACHUNN_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0574678.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45237..46793
                     /gene="gshA"
                     /locus_tag="ACHUNN_000184"
     CDS             45237..46793
                     /gene="gshA"
                     /locus_tag="ACHUNN_000184"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0574679.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            46943..47458
                     /gene="luxS"
                     /locus_tag="ACHUNN_000185"
     CDS             46943..47458
                     /gene="luxS"
                     /locus_tag="ACHUNN_000185"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0574680.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47522..49060)
                     /gene="emrB"
                     /locus_tag="ACHUNN_000186"
     CDS             complement(47522..49060)
                     /gene="emrB"
                     /locus_tag="ACHUNN_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0574681.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49077..50249)
                     /gene="emrA"
                     /locus_tag="ACHUNN_000187"
     CDS             complement(49077..50249)
                     /gene="emrA"
                     /locus_tag="ACHUNN_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR0574682.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50376..50906)
                     /gene="emrR"
                     /locus_tag="ACHUNN_000188"
     CDS             complement(50376..50906)
                     /gene="emrR"
                     /locus_tag="ACHUNN_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0574683.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(50997..51332)
                     /gene="ygaH"
                     /locus_tag="ACHUNN_000189"
     CDS             complement(50997..51332)
                     /gene="ygaH"
                     /locus_tag="ACHUNN_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0574684.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51322..52059)
                     /gene="ygaZ"
                     /locus_tag="ACHUNN_000190"
     CDS             complement(51322..52059)
                     /gene="ygaZ"
                     /locus_tag="ACHUNN_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0574685.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52183..53367)
                     /locus_tag="ACHUNN_000191"
     CDS             complement(52183..53367)
                     /locus_tag="ACHUNN_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0574686.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53658..54650)
                     /gene="proX"
                     /locus_tag="ACHUNN_000192"
     CDS             complement(53658..54650)
                     /gene="proX"
                     /locus_tag="ACHUNN_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0574687.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54708..55772)
                     /gene="proW"
                     /locus_tag="ACHUNN_000193"
     CDS             complement(54708..55772)
                     /gene="proW"
                     /locus_tag="ACHUNN_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0574688.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55765..56967)
                     /gene="proV"
                     /locus_tag="ACHUNN_000194"
     CDS             complement(55765..56967)
                     /gene="proV"
                     /locus_tag="ACHUNN_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0574689.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57323..58282)
                     /gene="nrdF"
                     /locus_tag="ACHUNN_000195"
     CDS             complement(57323..58282)
                     /gene="nrdF"
                     /locus_tag="ACHUNN_000195"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0574690.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58292..60436)
                     /gene="nrdE"
                     /locus_tag="ACHUNN_000196"
     CDS             complement(58292..60436)
                     /gene="nrdE"
                     /locus_tag="ACHUNN_000196"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0574691.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60409..60819)
                     /gene="nrdI"
                     /locus_tag="ACHUNN_000197"
                     /pseudo
     CDS             complement(60409..60819)
                     /gene="nrdI"
                     /locus_tag="ACHUNN_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60816..61061)
                     /gene="nrdH"
                     /locus_tag="ACHUNN_000198"
     CDS             complement(60816..61061)
                     /gene="nrdH"
                     /locus_tag="ACHUNN_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0574692.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61309..61638)
                     /locus_tag="ACHUNN_000199"
     CDS             complement(61309..61638)
                     /locus_tag="ACHUNN_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR0574693.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61790..62134
                     /locus_tag="ACHUNN_000200"
     CDS             61790..62134
                     /locus_tag="ACHUNN_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0574694.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62171..62620)
                     /gene="alaE"
                     /locus_tag="ACHUNN_000201"
     CDS             complement(62171..62620)
                     /gene="alaE"
                     /locus_tag="ACHUNN_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0574695.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14828 a        16243 c        16973 g        14952 t
ORIGIN      
        1 atggcatatt ttatgttgtc attaatgatt tcaataatat gtggaaggtg atggaatttc
       61 agcaacagat ctattgcctc ttgttgctct gaatcattta aatcgaaaaa ccagtcacca
      121 ccgctggcaa gccacatatt tccatcgcca gtttcgaatg catccaacgc catcgcttcg
      181 tggttgcctt taaccgacgt aaaccagggt tggtttagca ggcgcagcac gttaagactc
      241 ttcggcccac gatcaatatt atcgcctgtg gaaataagta agtcggtttc agggtaaaaa
      301 gagagttgat gttaacggga ttgtaataac tgatattcac catgaatatc accaacgacc
      361 catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg catggtttta
      421 ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt cagttgtcgt
      481 taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact cttcaagcga
      541 taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt ttccagtgct
      601 tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt accatccact
      661 tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg ccgtgcgcgc
      721 ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta gcttttgctc
      781 gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc cagcgcatcg
      841 agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc gaaatagtgg
      901 gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca cgcccacgcc
      961 agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa caccagactg
     1021 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
     1081 cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
     1141 atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc aatcagtgcg
     1201 gtctggcgca tataggtact tttaccgccc atgttcggac cggtgatgat caacatacgg
     1261 cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac ttgttcaact
     1321 accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt cgggcaggtg
     1381 tagttcaggg tataggcccg ttccgccagg ttcaccagca cgtcgagttc cgccagcgcg
     1441 ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacaa ctcttcataa
     1501 agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc ttcgtactct
     1561 tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg catgtagttg
     1621 atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg caccgcatta
     1681 aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc cagacgctcc
     1741 agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc gttatagccc
     1801 gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat tgctcgctcc
     1861 agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg taccggtgca
     1921 ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc gtggcgcata
     1981 cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat acgttccagg
     2041 tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa tgcgccaata
     2101 gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag ccagcgtttc
     2161 agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag cgtattttcc
     2221 gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc atccataatg
     2281 atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt cgtgcgttgg
     2341 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg cgcgttctcg
     2401 acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg gcgcgcggtg
     2461 tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc aatcagcgac
     2521 atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg cagctctgcc
     2581 gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct gatatccagc
     2641 gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt gtcctgacgc
     2701 tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac aactttgcgc
     2761 tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc aacggactct
     2821 ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat ccccgccatc
     2881 gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag cagttgcgac
     2941 gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta aaacagcagg
     3001 atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt atgggcgtcg
     3061 aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat ggggtgtgat
     3121 ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta atcatttcag
     3181 gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg ttttgcagct
     3241 tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa acctcctcct
     3301 tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta agagtatgga
     3361 ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa aatgatcgat
     3421 ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga ggcgttgttt
     3481 gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc agcctgtaag
     3541 cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca gattatgcgt
     3601 tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat
     3661 gacaagcgtc cggtaccaga actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag
     3721 tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg atttatcaat
     3781 tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac gttgcgcagc
     3841 gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgcacaa tcaactgata
     3901 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttccggtaa
     3961 aacaatatct ggcaatgaca gctgcagcac gttaccgcgt gttagcaata ccgcgcgctc
     4021 aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca aggtgcgcag
     4081 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
     4141 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg
     4201 gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt
     4261 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
     4321 gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta actccagtgg
     4381 catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa aacgaccaat
     4441 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctttccag
     4501 caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact
     4561 gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
     4621 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
     4681 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
     4741 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
     4801 atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat tggtagtggt
     4861 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
     4921 cagcggtaac aggcacaagg tttgaatctg gttgccctag gtgtcgaaca acatgcgttc
     4981 atagggggct aaatcgtccc gttcgtggag attgatcagc agcatctctt tacttttgaa
     5041 cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgttg
     5101 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
     5161 gatactgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt cgctgaccag
     5221 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
     5281 atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc tctggatttg
     5341 ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
     5401 ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
     5461 cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcga ccagctgcgg
     5521 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgcg
     5581 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggt gtctttttca
     5641 cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg
     5701 tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg
     5761 ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc
     5821 ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg
     5881 actaactata ggagatttag aattttatta gcatatacgc ggaagaggtt cggcgtgtgg
     5941 taaatcgagg gttcgtttca caccatacag accggcaaga cgaacacctt tacgttccac
     6001 cacttcacca attagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac
     6061 ttgctctgcc gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttaag
     6121 ggcgtccagt cccagcagtt cgcaaacgcc gcgcacggca ggtttaaccg gcagtgccac
     6181 ttctgaaatt tcaataccac aaccgcaggc tgccgcgaac tcatgaacta ccgcgttcac
     6241 accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat
     6301 aagcggcgtc agtaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg
     6361 caggttaagg atagtcgccc cgtggtcccc gagcgtgccg ctaaccagta atacatcgcc
     6421 tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt
     6481 gttgataaac agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat
     6541 ggcaatgcct gccgcgcgcg cggtttctgc catgctggtc actacggctt tcagtgtctc
     6601 catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact
     6661 gaccgcaacg tcattggctg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa
     6721 gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttctgctac
     6781 cagctgcgcc agatcaagac gtgcctggtc ttcctgctct gccagccacg ggttggcaaa
     6841 ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc
     6901 gagttggata ttattcacgc ttcattctcc tgctgacgat actgatacca cgcggcgcac
     6961 gctccttcgg aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca
     7021 aacagcgggc attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc
     7081 gggtcatcgc agacctgctg cggtgctggg cggaaatgtg cttcggcatc gaatcgttga
     7141 taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg
     7201 tcgccgttga cacagaacac atcagcaatt gcctgttgcg ccagcaggtt accggcatcc
     7261 ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc
     7321 agcatgacca cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga
     7381 tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc
     7441 ggcgcgagga acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg
     7501 ataagcgtaa tatgctggca gaagaagtaa aaattctgca catcgcgggc tttcgcctgt
     7561 tgcagggtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaatacgact
     7621 ttacgggttg gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc
     7681 acatcggcac cgcgtgcttt tgcctgcaac agcgatccct gtttccccgg cacgcgcatg
     7741 gcgtcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg
     7801 attctaccca tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt
     7861 tcaggcagta actggtcgag gccgaattta aaaatggcgt gggtatgacc accgcacact
     7921 tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga
     7981 tgctcaatta actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt
     8041 tttcctcgcc atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg
     8101 cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgta
     8161 ccagtaccca ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta
     8221 aatcgacatc gcgctgaatg ccgcagacgt cgacttttgc ctggttgccg tcaatggtgc
     8281 ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc
     8341 catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg
     8401 ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt
     8461 gagcagcatc agcgaggcgg cggcaaacat atgcggatat ttcagcggtt tgtcttcacc
     8521 ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc tggccgggca
     8581 tacgaggttg ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg
     8641 tgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat
     8701 cgctggtgtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcaccttc
     8761 aataaccgcg cacggaacgc tgtctttcag gcgcattagg gtttccgtca gcagggtggt
     8821 tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg
     8881 cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt cgacttccag
     8941 catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac cgtaatgcag
     9001 gtcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat
     9061 cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc
     9121 atcaccctcg atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc
     9181 ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc
     9241 accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca
     9301 tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac
     9361 gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa
     9421 tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg
     9481 ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa
     9541 ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg tgaataatgt
     9601 cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct
     9661 atttaataac aataaattaa aagttggcat agaaaatgct taaagctggc atctctgtta
     9721 aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca
     9781 gcggcattgt cgcctacagg accagtggca catctactgc aattcgctgg ttcaggggat
     9841 cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg
     9901 tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca
     9961 caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc
    10021 gcaactgagc attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt
    10081 tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgctattga
    10141 aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc
    10201 tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg
    10261 tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc
    10321 tggtgaatcg ttttgtaatt gttgactcca cgctctgtat cggctgccac acttgtgagg
    10381 ccgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga
    10441 tgctgaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgtg
    10501 cggtggtttg cccggttaac gccatcaccc gcattgatgg ggccgtgcag ttgaatgaaa
    10561 gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt
    10621 ccggcagccg tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac
    10681 cgcctgctcc ggcgcgtatc agtacgctgc ttgactgggt gccgggtatt cgcgcgatcg
    10741 ccgtcaaatg tgacctttgc agttttgatg aacaaggtcc ggcctgcgtg cggatgtgcc
    10801 cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc
    10861 gtgagctgac ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg
    10921 gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc
    10981 cgctgttctg gcatttctct tttcttttca aaaagcgtta agtggctgga tagctggaat
    11041 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
    11101 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ctccgcttaa
    11161 cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct acaacattga
    11221 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
    11281 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
    11341 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
    11401 ggggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
    11461 cacgctggat ctgtgcctac tggatatgcg tatgcaacag ctgccgctcg gttccgatat
    11521 ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc tgcacggctg
    11581 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
    11641 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
    11701 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
    11761 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg
    11821 cattatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct
    11881 gattgctctt ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag
    11941 cgtactgttc ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa
    12001 actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat
    12061 ggcaatggct gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc
    12121 cttcttcaaa ctgagcaata gcggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc
    12181 tgtgggtctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt
    12241 cacgttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct
    12301 gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc
    12361 gtggctgctg ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac
    12421 cgtttctcaa ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat
    12481 tatggcgatt tgcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg
    12541 cgactacgac catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa
    12601 acaggcgttt gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc
    12661 gggctggcag tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc
    12721 ggtactggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta
    12781 attcaggcgc tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg
    12841 cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg tgacattttc
    12901 aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt ccgcctgacg
    12961 ccgtatgtga tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc
    13021 ggttctccgc tgccgcaact gggtgattta atcaccttac tgtatctctt tgccatcgcg
    13081 cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc tatcggcgcg
    13141 agccgtgaag cgatgcttgg cgtgctggtc gaaccgatgc tgctgcttgg tctgtgggtt
    13201 gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt ttatcactgg
    13261 ccgctgagcc agagcatccc gctggtactg gcgctttgtg cctgtgcgtt cgccaccttt
    13321 atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagct gcaggaaggc
    13381 ccgctctctg aatacagcgg cagcggcttt ggcgtcatga aatggggtat cagcctgaaa
    13441 cagctggtgg tgctgcagat gttcgtcggc gtatttatcc cgtggggaca gatggaaacc
    13501 ttcaccgccg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact ggtcgtcggc
    13561 gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg
    13621 cgcattacct ggactgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg
    13681 tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc
    13741 tgaatgaggc atttccgggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga
    13801 ctgtcaccgt aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg
    13861 ggggctggct gtcggtgctt tttggtaacg acgaacgcaa actgaatggt cattacgccg
    13921 tttattacgt gctgtcgatg gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag
    13981 ttgatgccaa caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg
    14041 gcgagcgcga agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgcc
    14101 gtctggtgct gccggatgaa ctttatccgc tgcgtaaaga cagcatggat tatcgtcagc
    14161 gtccggcacc gaccaccgat gctgaaacct acgagttcat caacgaactg ggcgacaaga
    14221 aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc tgacgaaccg ggccacttcc
    14281 gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgtctgttc tacgttcatc
    14341 gcggcatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc ttcctctctg
    14401 accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg tcggtagaaa
    14461 acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt ctgctggagg
    14521 tggaacgcct gcactcgcat ctgctcaacc tcggtctcgc ctgccacttt accggctttg
    14581 actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg gcagagatcc
    14641 tcaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt cgcgatctgt
    14701 tgaaagatga catgatccag acccgccagc tggcgcaaca gatgcgtcgt gaagtgcagg
    14761 agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcaccgtc ggcattggtc
    14821 gtctggaccc ggaaatggcc cgcgatttca gtaacgttgg cccgatggtt cgcgccagcg
    14881 gtcacgcccg cgatactcgc gccgatcacc cgtttgtcgg ttatggcctg ctgccaatgg
    14941 aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt atcaacgaag
    15001 tttataccgc gctgaacatg atcgactacg gtctggataa cctgccgggc ggtccgctga
    15061 tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc gaagcgccgc
    15121 gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac cgctggcgct
    15181 gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc ggtaacaccg
    15241 tttccgatgc gccgctgatt atcggtagct tggacccttg ctactcctgt accgaccgca
    15301 tgaccgtggt cgatgtgcgt aagaaaaaga gcaaagtggt gccgtacaaa gaactcgagc
    15361 gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg tttaccttta
    15421 tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag ccgattgcgg
    15481 ttgataaaaa cttccgtggt aagccggagc agaacccgca gcagtgcatc ggctgtgcgg
    15541 cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc gccaccggtg
    15601 agctggcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc gaagaagttt
    15661 gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcagtgtgg aagaaagaag
    15721 acttcctgca acagtcccgc ttcgcgctgt gcaactgccg cgtctgcaat cgtcctttcg
    15781 ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc gacagccgcg
    15841 cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag aaatgcctgg
    15901 tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga tgagcaattt
    15961 attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg aatccatcgc
    16021 cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt atcgcgtgga
    16081 ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc cgctgtttga
    16141 tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata ttttactgtt
    16201 taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc agtccgcgcc
    16261 ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta ttttccacga
    16321 tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct acattcctgg
    16381 ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg gcctgctgga
    16441 gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caacaggcgg aaatcctgca
    16501 tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc gcctggcggg
    16561 ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc agggcgaaga
    16621 acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga ttgtcagcca
    16681 tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt gttcagtcaa
    16741 ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca gcaggtggtc
    16801 tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga agcggcgctc
    16861 acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg cagtgaagcc
    16921 cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat taaccatgtg
    16981 ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca gcagaaggaa
    17041 tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc catctatttg
    17101 atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca tgatgggcga
    17161 tgatggcgct ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag gtaactgggt
    17221 ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg aactgcgccc
    17281 tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg aaatccgcat
    17341 catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca tgccgctgaa
    17401 ctatcttatc gaccagttga aagaagacat tggcgaagtg attttcctcg gcatccagcc
    17461 ggatatcgtc ggcttttact acccgatgac tcagccgatt aaagatgcgg tagaaaccgt
    17521 ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag cggtggaaga
    17581 agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga tgtgcgcttt
    17641 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    17701 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    17761 cgagcaaacc tggcatttta gaaaagtggg cgttgctggc gtgacgcttg aaaagatgcc
    17821 aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat cctggtctgt
    17881 gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg ccgatcttga
    17941 tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg gtctggctcc
    18001 ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg tctttgaacc
    18061 caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt tgcaaggcaa
    18121 tggtcacacg caattgctgg atatgcagtt tgattccggt tactggataa ccagtctgta
    18181 tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt taagctatcc
    18241 cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac ccatcgccgg
    18301 gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa agggggatgt
    18361 tcttgatggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    18421 attatatcgc gctcaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    18481 gaacacataa ggatagaact cgcagatgtg gctgtttact tactataagg aagttaaaac
    18541 ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa atatcctaac
    18601 gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt ttctacgcca
    18661 gaagagatgc gaacggcgtt tcccaacctc gacaatttta aatatcgcaa caaatggtac
    18721 gttctggatg taggtgggaa caacctaagg gtgattgcct acataaactt tgttaataag
    18781 cggttctttg tgaagcacat aactaatcac gccgagtatg acaaactgac acgttactac
    18841 agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca agagaactgg
    18901 tcaatgcagt cccctttctg ggaggtagtg actcagaaga tgattatcgt gaagcactgg
    18961 aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac tttctggcga
    19021 gccgcattgc agaatatgaa aacaacaacc aagagtttgc agagttcgac aaagccgttg
    19081 cggcaatgcc tgttggcgtg gcgttacttc gcacgctgat tgatcaacat aatctgacct
    19141 atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt ttgtctggac
    19201 aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc gttaagccgg
    19261 aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg ctgcgcttat
    19321 cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc gcatccggca
    19381 ctgttaccta ctctaaatct tccccattac tggcaatcac ttttttatac caccagaacg
    19441 atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct acgtagacaa
    19501 aaccgtagcg tttgctcatt tcacccgtag aggcggagac taaatcaata cagccccagg
    19561 tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg cggatatgtt
    19621 cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca agttcatctt
    19681 ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg tacatcatgt
    19741 tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc acttgcagat
    19801 acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg ttcatctccg
    19861 ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg tttttcagga
    19921 tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat acgcgggcag
    19981 agtaagccgg atacgtgccc cgcgcctgca catcgataaa aaacaggttt tcccggtctt
    20041 tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag ttaccgcccg
    20101 ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg gtggctagcg
    20161 cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga ttttcacctt
    20221 cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg atttcattga
    20281 aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg gcgtagcggc
    20341 tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc agatgcatcg
    20401 gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta cactcttcaa
    20461 agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg ccctgcggaa
    20521 agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct gccatcaggg
    20581 cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga taaaactcgt
    20641 catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga tgctcgccgt
    20701 gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca ccttcagact
    20761 ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac attttcatcc
    20821 tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct ttcgcgcctc
    20881 ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga ggataaacga
    20941 cagcaccacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg gattcgccgg
    21001 atcgaagaat tgcacgctgg taaataaccc tggcgctgcc attgagtggc tggcaagccc
    21061 cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga taagcggacg
    21121 tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca taatggctga
    21181 tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc acgccaccgc
    21241 cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga ccatcccttc
    21301 tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt gcatcccggt
    21361 cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc ccagataacc
    21421 atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa tcgggccaat
    21481 cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga ggaagttttt
    21541 ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc accaggtcat
    21601 caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa tcagggcaaa
    21661 ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg gatgtaccag
    21721 cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg cggcagatgc
    21781 cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca gaatggttaa
    21841 ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca gcagtttgac
    21901 catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca gcgcatcgag
    21961 gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct gtgcgggctg
    22021 catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg tattgccgat
    22081 aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga tggtttttaa
    22141 cgtcgggctg tcgataagct ggtcatcttt gataacaacg cgcaaacgcg tcatacagtg
    22201 cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc gtgccagcgc
    22261 cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg tagttgttat
    22321 cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg gtttcacaaa
    22381 atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt tatatttgtg
    22441 aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc aaacggtgca
    22501 gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc tggaagtggc
    22561 gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta atggctacgt
    22621 cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt accgcccaaa
    22681 cttgctggcg cgcaatctgt cggcgaagag tactcagacg ctggggttgg tagtgaccaa
    22741 cacgctttac cacggcattt attttagtga attactcttt catgccgcgc gaatggcgga
    22801 agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag aagagcgcca
    22861 ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc cgcgtttttt
    22921 aagcgtggat gagatcgatg acatcattga cgcgcacagt cagccgataa tggtgcttaa
    22981 tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac agaccagctt
    23041 taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc ttaccggctc
    23101 gatggattcc cccaccagca ttgaacgtct tgctgggtat aaagacgcgc tggcgcagca
    23161 tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg ccagcggggc
    23221 cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag ttgccagtaa
    23281 cgacgatatg gcgataggtg cgatgaaagc gttgcacgag cgcggcgtag cggtgccaga
    23341 gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg ttccggcgct
    23401 ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac ggctgatttt
    23461 tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac tgatccgccg
    23521 cgactcactc attgctcttt cgcgataaac attgtcatca catgtcatcg tcactagtgt
    23581 cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca gtgaattaaa
    23641 actggcatga tttgtgaatg tatcggcgca ttaactgtca ttgctggaga atttgatgaa
    23701 ccgtttcatc attgctgacg cgagtaaatg tattggttgc tgtacctgtg aagtggcgtg
    23761 cgtggtttct catcaggaaa atcaggactg tgcatcgctg accccggaaa catttttacc
    23821 gcgtatccat gtcattaaag gtgtgaacat ttccacggcg acagtttgcc gtcagtgtga
    23881 agatgcaccg tgcgctaacg tctgcccgaa tggtgctatc agccgtgata aagggtttgt
    23941 tcatgtgatg caggaacgtt gcattggttg caaaacctgc gttgtggctt gcccgtatgg
    24001 cgcgatggaa gtggtggtac gtccggtgat tcgcaacagc ggcgcggggc tgaatgtacg
    24061 ggctgacaaa gccgaagcca ataaatgtga cctgtgcaac catcgtgaag acggcccggc
    24121 gtgtatggcg gcttgcccga ctcatgcgct gatttgtgtc gatcgtaata aacttgaaca
    24181 actgagtgca gaaaaacgcc gccgtacggc gctgatgttc taaaaatctt acagaactgt
    24241 tctggcatca aaggaattcc cggaaatatt cgtagaatac ggagctggcg gtatcgccga
    24301 cctgagactg ccggatacga cgcagtcgtg tccggcagga cgggttacgc aattcatgat
    24361 ggcgggaata actcaatggc aaaaaacaca tcttgcggtg tccaactgcg tattcgtggc
    24421 aaagtgcagg gcgtcggttt tcgtccgttt gtctggcagc tggcacagca attaaatctt
    24481 cacggcgatg tctgtaatga cggcgatggc gtagaagtcc ggctgcggga agacccggaa
    24541 acgttacttg ttcaattgca tcagcactgc ccgccgctgg cgcgtattga tagcgtcgag
    24601 cgtgagccgt acatctggtc acaactgccc actgagttca ccatccgcca gagcgcggga
    24661 ggtgccatga atacgcaaat tgtcccggat gccgctactt gccctgcttg ccttgccgaa
    24721 atgaataccc caggcgaacg gcgttatcgt tatccgttta tcaactgtac ccactgcggc
    24781 ccgcgtttca ccattattcg cgccatgcct tacgaccgcc cgtttaccgt gatggcggcg
    24841 tttccgttgt gtccggcttg tgacaaagag taccgtgacc cgctcgatcg tcgcttccac
    24901 gcccagccgg tggcctgccc ggagtgtggc ccacatcttg aatgggtaag tcatggtgaa
    24961 catgcagaac aagaggcggc attacaggcg gctatcgcac agttaaaaat gggcaacatt
    25021 gtcgccatca aagggattgg cggatttcat cttgcctgcg atgcacgtaa cagtaacgcg
    25081 gtggcgacac ttcgggcgcg caaacatcgc ccggcgaaac cgctggcggt catgttgcca
    25141 gtggctgacg gtttaccaga cgctgcgcgc cagttgctta ccacgcccgc cgcgccgatt
    25201 gtgctggtgg ataaaaaata cgttcctgag ctttgtgatg atatcgcccc tgaccttaac
    25261 gaagtcgggg taatgttgcc tgcgaacccg ctccagcatt tgctgttaca ggaactgcaa
    25321 tgcccgctgg tgatgacctc cggcaacctg agcggtaaac caccagctat cagcaacgaa
    25381 caggcgctgg cggatttgca gggcattgcc gacggattct tgatacataa ccgcgacatc
    25441 gtgcagcgga tggatgattc ggtggtgcgc gaaagcggcg aaatgctgcg ccgttcgcgg
    25501 gggtatgtgc cggatgcgct ggctttgcct ccgggcttta aaaatgttcc gcctgtgctg
    25561 tgtctcggcg cggatctgaa aaataccttc tgcctggtgc gcggtgaaca agcggtgttg
    25621 agtcagcatc tgggcgattt aagtgacgat ggcatccaga tgcagtggcg cgaagcgtta
    25681 cgcctgatgc aaaacatcta cgattttacc ccgcaatacg ttgtgcatga cgcgcatccg
    25741 ggctatgtct ccagccagtg ggcgcgtgaa atgaatctgc cgacgcaaac ggtactgcat
    25801 catcatgccc atgcagcggc gtgtctggca gagcatcagt ggccgttgga tggcggtgat
    25861 gtcattgctt tgacgctcga cggtatcggt atgggggaga acggcgcttt gtggggcggc
    25921 gagtgcctgc gggtgaacta tcgcgaatgt gagcacctgg gcggcttgcc tgcagtggcg
    25981 cttccgggtg gcgatttggc agcgaagcag ccgtggcgaa acctgctggc gcagtgcctg
    26041 cgctttgtgc cggagtggca gaattaccct gaaacagcaa gtgtgcaaca gcaaaactgg
    26101 agcgtgctgg cgcgggccat tgagcgtgga attaacgcgc cgctggcgtc atcgtgtggg
    26161 cggttgttcg atgcagtggc ggcggcactg ggctgtgcgc cagccacgtt aagttatgaa
    26221 ggtgaagcgg cttgtgctct ggaggcgctc gcagcctcat gccacggagt gacgcatccg
    26281 gtgacgatgc cgctggtgga caatcaactg gatctcgccg ctttctggca gcagtggctg
    26341 aactggcagg caccggttaa tcaacgcgcg tgggcgtttc atgatgcgct ggcgcagggt
    26401 tttgccgcgt tgatgcgtga gcaggccacg atgcgtggta tcactacgct ggtatttagc
    26461 ggcggggtta ttcataaccg tttgctgcgt gcacgtctgg cgcattatct cgctgatttc
    26521 acattgctct ttccacagag tttaccggcg ggtgatggcg gtttgtctct ggggcagggg
    26581 gttattgctg cggcgcgttg gttagcgggt gaagtccaga acggataaaa cgtgttgcat
    26641 ttggacacca ttgccggatg cggcgtaaac gccttatctg gcctacacat gcgtccccgt
    26701 aggtcggata agacgcgcca gcgtcgcatc cgacatttta ggcacagtag cccacctaca
    26761 tcggcaatgt tttcaacaat ccaaatgcct ctttcatccg atcctcactg accacaaagg
    26821 cgcgaagctg gtcagcatcg tcaacaccgc gagccaccat tccctggcgt tcggtattaa
    26881 tttgccagcg taaatcctga cgctgggttt cgcctgccag atgcagcggt aattccggcg
    26941 ttttgatttt caccagcatc gccggtagtt tcagcggcgt gttattgccg agaagatttt
    27001 ttgccagcac catcgcgcta agttgaatcg gctgcaggaa tggcagtacc tgaccgttga
    27061 tttccgcgca atcgcccagc gcataaatat cgtcattact ggtttgcaga taactatcga
    27121 cgcaaacgcc gcgattaatc gtcagcccgg cgcgtcgtgc cagggctgtt tccgggcgca
    27181 gtccggtggc ggcaattacc gcatccactt cgatactacg ctggtggtcc agcgttgcca
    27241 gaatgccaga atccgttttt tccagcccct gcaactgaga tttcaacagc agatgaacgc
    27301 ccatctccgt caaccgatgc tgcaagcggc tgcttacttc tggtggcatt aatgacgcca
    27361 gaatactggc agcgttgtcg attagcgtga ccgctttgcc tgcacgacaa aaatccatcg
    27421 ccagttcgct accaatcaaa ccaccgccga caatcaacac gcgtcgggca tcccgcagtt
    27481 gcgtttcaca ggcgcgatac tcttgctgac tatttaacgt cagcattaac tcacgcccag
    27541 gcactggcgg gacaaaggca ctggcaccag ttgccagcac cagcttgtcg tattgccact
    27601 gattattctg gcttttcacc acatgggctt cggcatcgat atccgtcacc caggtgtgcg
    27661 gaaacaggtg cagattaaac tgcccggcaa attcacccgc cgtctggcgg gtaaggtcgt
    27721 cggcacgttg cccctggctg ataacatggc tgaggtccgg tttgttgtac tcatccatgc
    27781 tgtcggcggc gatcagggtt aatggaatac tggcgtcctg tttgcgaata tttttcacca
    27841 gttggcgggc ggcgaagccc gaaccaatga tcacaatgcc gttgctcatt ttgcctccga
    27901 tgccagttcg tcaaagacgt ctttgccgag agagcattcc gggcagagga agttatccgg
    27961 aacttcactc cacggcgttc ctggcgcaac gtcctgcatt ggctcgcctt ttgccggatc
    28021 gtaaatccac tggcagacgc tgcactgcat ccgtgggccg aggtcggact tcgtggtggc
    28081 agaggtttct tctttaacca ccgtattcac cgtgctctgc ggcagcggcg cgagcgccca
    28141 ctgacgggcg atttcgcgac catgttcacg gcataactcc agagcgtcct ggtctggtcg
    28201 ccatttggct ttcaggctaa gcgacatttc gaaacccgca tcctgcaggc gcgtggaaag
    28261 acgatccacc gcaccgccgc tccagccgtg agagccgaaa gcactggcgc gtttgttacg
    28321 gaagcgtaat ccggtcatct cctccaccag accggcgatt ttcggcatca tcacgttatt
    28381 catcgtcgaa gtgccgacca gcacgccttt tgagcggaag acattggtca ggatttcgtt
    28441 tttatcgctt cgggcgacgt tgaaaatttt caccgccacg cgtgggtcgg tttccgcaat
    28501 cccctgggcg atagcgtcag ccatcatgcg ggtgttattc gacatggtgt cgtagaaaat
    28561 ggtgattctg tcttcctgat aatcagccgc ccatttcagg tacagctcga caatttgcgt
    28621 cgggttatcg cgccatacca cgccgtgaga ggtcgctatc atatcgactg gtaagttaaa
    28681 gcccaggatc tcggtaattt tcggtgttac caggcggctg aacggcgtca ggatattggc
    28741 gtagtaacgc tggcactgct cgaaaagctc cgtctgatcc acttcatcgt tgaacagatg
    28801 ctcgtcgcag tagtgctgac cgaaagcatc gttactgaac agcaccgcgt cgcctgtcag
    28861 gtaagtcatc atgctgtccg gccagtgcag cattggcgtt tcgacaaaaa tgagctgttt
    28921 gccgttgccg atatccagcg tgtcaccagt tttcaccaca ttaaaattcc actccggatg
    28981 atgatgatga ccatttatcg agtcgatagc gttggcagta cagtaaatcg gcgtatcggg
    29041 aatttgcgcc atcagttcgg tcagcgcccc agcgtggtcc tcttctgcat ggttaatcac
    29101 gatgtaatcg atatccgcca gatcgatttc attacgcagg ttctgcacaa attcgcggct
    29161 gaatttatgg tcgacggtgt cgatcagcac gtttttttct tcgcggatga ggtagctgtt
    29221 gtaactgctg ccgcgtagcg ttttatattc cgtaccgtga aaatcacgca cttcccagtc
    29281 acgttgacca acccaatgaa tgttattttt caccacaata gacatagcaa cctcaattta
    29341 ttcagcgtgt tctaaaaaga tgtcttgcta attgcagatt acgtgccagt tttttatctg
    29401 attgttttta ctctataaat aaaattatgc tcattaatga gtagtcaaaa tgactatcaa
    29461 tcaaatggtc gatatgacaa tatctatagt caaattgaca gtgaggcaaa gatgagtttt
    29521 tccgttgatg tgctggcgaa tatcgccatc gaattgcagc gtgggattgg tcatcaggat
    29581 cgttttcagc gcctgatcac cacgctacgt caggtgctgg agtgcgatgc gtctgcgttg
    29641 ctacgttacg attcgcggca gtttattccg cttgccatcg acggtctggc aaaggatgta
    29701 ctcggtagac gctttgcgct ggaagggcat ccacggctgg aagcgattgc ccgcgccggg
    29761 gatgtggtgc gctttcccgc agacagcgaa ttgcccgatc cctatgacgg tttgattcct
    29821 gggcaggaga gtctgaaggt tcacgcctgc gttggtctgc cattgtttgc cgggcaaaac
    29881 ctgatcggcg cactgacgct cgacgggatg cagcccgatc agttcgatgt tttcagcgac
    29941 gaagagctac ggctgattgc tgcgctggcg gcgggagcgt taagcaatgc gttgctgatt
    30001 gaacaactgg aaagccagaa tatgatgcca ggcgatgcca cgccgtttga agcggtgaaa
    30061 cagacgcaga tgattggctt gtcccctggc atgacgcaac tgaaaaaaga gattgagatt
    30121 gtggcggcgt ccgatctcaa cgtcctgatc agcggtgaga cgggaaccgg taaggagctg
    30181 gtggcgaaag cgattcatga agcctcgcca cgggcggtga atccgctggt ctatctcaac
    30241 tgtgctgcac tgccggaaag tgtggcggaa agtgagttgt tcgggcatgt gaaaggagcg
    30301 tttactggcg ctatcagtaa ccgcagcggg aagtttgaaa tggcggataa cggcactctg
    30361 tttctggatg agatcggcga gttgtcgttg gcattgcagg ccaagctgct gagggtgttg
    30421 cagtatggcg atattcagcg cgttggcgat gaccgtagtt tgcgggtcga tgtgcgcgtg
    30481 ctggcggcga ctaaccgcga cttacgcgaa gaggtgatgg cagggcgatt tcgcgctgac
    30541 ttgtttcatc gcctgagcgt gtttccactt tcggtgccgc cgctgcgtga gcggggcgat
    30601 gatgtcattc tgctggcggg gtatttctgc gagcagtgtc gtttgcggct ggggctctcc
    30661 cgcgtggtat taagtgccgg agcgcgaaat ttactgcaac actatcgttt tccggggaac
    30721 gtgcgcgaac tggaacatgc tattcatcgg gcggtagtgc tggcgagagc cacccgcaac
    30781 ggcgatgaag tgattcttga ggcgcaacat tttgcttttc ctgaggtgac gttgccgccg
    30841 ccagaagcgg cggcggtgcc cgttgttaag caaaacctgc gtgaagcgac agaagcgttc
    30901 cagcgtgaaa ctattcgcca ggcactggca caaaatcatc ataactgggc tgcctgcgcg
    30961 cggatgctgg aaaccgacgt cgccaacctg catcggctgg cgaaacgtct gggaatgaag
    31021 gattaaataa tcccggcctg atagaaatcc tgcaggttaa ttgcgccggt gagtttgccg
    31081 ttttcatcca ccaccggtgc ggcagtgatt ttgcgcttca tcaggatctc tttggcgtcg
    31141 atggcgcgac tttgcgcctg taacgtggtg ccgcctgtcg tcatcgcttc attaactggc
    31201 gtggtgagtg cgccgccgcc taccagccag cgacgtaaat cgccgtcggt aaagacgcct
    31261 tgtacctgtt gttgagcgtc acataccgcc accagcccca gaccggtgcg gctgagttcc
    31321 agcatcgcat ccatcacgct ggcggttaac gccacctgtg ggatggcatc gtcacggcgc
    31381 atcagatgat gcactttatt cagcaagcga gcgcccagtg ccccggctgg gtgggagcgg
    31441 gcaaaatctt cttcattaaa tccgcgcgcc tgcatgaccg ccatcgccag cgcgtcaccc
    31501 atcatcaggg tattgacggt gctggaggtc ggcgcaaggt gcatcgggca ggcttcgcgt
    31561 tctacggaga tatccagcac cgcttttgcc gccaggccca gcggtgacgt cggtttgccg
    31621 gtcatcgcca gcagcgcgat agatttatct tccagacgcg gaataatcag atccagttcc
    31681 ttcgcgccac cggagtaaga gataaacagc atcacatcgc ggctttcaat catccccaga
    31741 tcgccgtgca gtgcttctgc cggatggaca aaaaaagccg atgtgccggt actggcaagt
    31801 gttgcggcga ttttcttacc aatgtggccc gatttgccaa ttcccgaaac caccactttg
    31861 ccttcgcagt gcaggatgat attggcggcg cgaacaaaat catcgcccag acgttccggt
    31921 aaacggcttg cttcctgcaa ctccagcatt aacgtctgac gtcccgcgtt cagtagtgct
    31981 tcactcattg ctctctccgg ttatgatcac atcgatccct ttctcttcca gcgcctgacg
    32041 aaacgccgga tcgatacctg cgtcggtaat cagcttatcg acgctttcaa gactgcaaac
    32101 tacgttgggg cttttacggc caaactttga tgagtccgcc atcaaaatca cttcgcgcgc
    32161 ggcattgcac attgccttac tgacggtata aacctcgtta aaggtggtta cgcccgcatt
    32221 gagatcgatg ccgtcggtgc ccataaacaa tttatcgaaa ctgaaatgtt cgaaggcatt
    32281 ctctgccagc tgtccgtgaa atgaggccga ttttttacga aacgttccgc ctggcatcag
    32341 gatagtttgt tcgttatcca gttcagatag cgcattgacg atatgcagac tgttggtcat
    32401 caccgtgatg ttattaaagc gcgagagcag gggaaccatc tgcaaaacgg tactgccagc
    32461 atcaagaatg atcgaatcgc catcatgaat aaaactaacg gcagcttctg ctatcagctc
    32521 tttcttgtgg gtgttgatga gtgttttatg atcgataggc ggatcggatt cctctttatt
    32581 caacaccact ccgccataag tacgaatgac ggttcctgca tgttccagaa tgaccagatc
    32641 tttgcgaatg gttgtgcctg tggtgtcaaa gtattgcgcc aattcttcaa ccgagcattt
    32701 accctgcttt tgcagatact ccagaatggc ggcctgacgc tgacgaggtt tcatgggcgt
    32761 gattacctga tttaggtttc aaaatgataa cttcgcaagc aagtaacatt cacaacgaaa
    32821 ttatccacgt ttcagtttaa gcgccaatga tagagcattc tgtgatagtg gatcatgggg
    32881 aaagatcaca tcgggctgta aatcacccgc atgcataccg gtaattgcgg gaattttttg
    32941 cggtcgtgcg aagacggtca gtcctttcat aaacaaggtg tcgacgatgc gctgctgatc
    33001 gtccagggcg atggcgacca cgacccgtgg tttaaagcgc ccgctggaac ggcccacgcc
    33061 aacccgccct tgctggcata gtgtgtcgaa ggcacggtta aaacgagaaa tttgccagcc
    33121 gcctaaggcc agttgcgcac accaggcgat aacggcgacg gtgatgagtg cggataccat
    33181 aacttttcct taatgtagtg gcggaagtcc tgtatgcctg atgagcgtag cgcatcaggc
    33241 attctattag tctcacggag atccttaacg gatctccgct gttgattaca acatcacctg
    33301 accgccggtg acattgatcg actgtccggt gcagtacgac gccttaggac tggcatagag
    33361 cagcagcata ttcagcacat cctgataatc gcagccgcgt ttgagcggta ctttgtcgat
    33421 gtaatactgc tcgacttgat ccggtttgat acccagcttg gtcgcgtatt ggggtaacag
    33481 tgactggaac ataggcgatt tcagcaggtt accgagcatc agtgaatgca ccgtaatgcc
    33541 gtactccgcc agatccagcg ccagtgattg agtcagcccg acgccaccaa atttcgctgc
    33601 gctgtagcca gagttgtgtt tgctgcccac tttgccggat ttcgagttga tctgaataat
    33661 gcgaccctga atcccgtcgc ggatcatcaa acgagaaaat tcacgggcac acaggaaata
    33721 acccaccaga ttcacggtca aaatcgccga gctggaagtc gctgataaag gctgcttttg
    33781 ctattccggc gctgtagacc agcaaatcca cgcgcccaaa gatttcatct accccacgag
    33841 agagcgccag gacgctttgc tcgctggtgg cgtcagcacc aaaactatac gctgttcctt
    33901 caccatattc ggcgttaatt tcttgtgcca catttgcggc tttgtcgctc tgaatatcga
    33961 caaccgcgac gcgatacccc tcggcagcca gaccgtggca caggaacgcg cctaaggttt
    34021 gcccaccacc gatgacaacg gcaacctgat tcatttttta ctccttaaca gattcaaact
    34081 tcaaaaccga tcccggcgcg atatcgtcgg gtacagggcc tgccacatgg acagtgcccg
    34141 gaaattccgc ttcgtttaaa ccatcgaagc gcagggtgac atgacccagt tcgcgaaggt
    34201 tgtcttccgc cacgctgcca acagcggtca ccggatagcg atgctgcccg agcgtaaatt
    34261 gcaaaccggg atggagtgca cctttcaact cgccgtggca atgaatgaag caatactctt
    34321 cgaggtccgc tggcgcgcct tcacgaaagg tgatgagcat ctggtcgctg agggcgtcag
    34381 tggcactcgc gccgatacgg gtgatggtgg tctgataaat aacggtcatg tttcagcctc
    34441 tattgataga taaaaccgga gacaaaccag gcgatcagta cagtcggtgc gccggttaaa
    34501 aagtggctca ccagtacaga agggacaccg acgcgaaccg tgtcctgacg ggcttccgcc
    34561 agcgacaaac cgaccgggat gaagtcgcag gccgcctgcg cgttgatggc aaacagtgcc
    34621 ggtaaagcca gatgcggcgg aatattgccg agaccaatct gcacgccaat caatacgccg
    34681 ataacctgtg cgataactgc gcctgggccg aggaaaggtg aaagcagcgg gaaggagcag
    34741 atgagcgcca gcatgaccag acctagtgga tggctcgcca gcggagcaag accgtgggca
    34801 atccagtcac caaggccaga agccataatg atgccaatga gcgccgagac gaacgccata
    34861 aacggcagaa tggtttttaa tacagtgtcg atggtgtcac gaccagattg aaacaacacg
    34921 gcaacggcag aacccatacc cattcccacc ttcgccagta aaccatcgct ttgttcggtg
    34981 attttcttgc tggtgtcata gtcacggccc acggaagagg gttgtggtgt cgcatcaccc
    35041 actacagtga tgttttcttc ttttacgcca gagacataaa tatcttccac aatgtactgc
    35101 gccagcggac tggacttgcc cgtcgagtgg atattaatgg tgggaatacg tcgtttcgga
    35161 tagatgccgc agcgtaatgt gccgccacag tcgattaccg cgacaccaat ttccgcctcc
    35221 gcgggttcac cttctttaaa tccgtcaata gcctgccagc cagtaagttg tgccagtttg
    35281 tcaacaatcg caggccgggt accggcggtg atatagacga tttttttgcc cggcgtggct
    35341 tccagctcaa gcgggccgcc ccagccaccc gttccttttt cgatccgaat acgcgtcatg
    35401 atgttgctcc tgccaggtga actttttgtt caagttgaat gcccatcttt ttctcaaaaa
    35461 tggcggtggt cagatcggtc acccagccgc gaaagaagtt ggtgaccaga ccaaccagca
    35521 gatagctcac cgccagtggg ccaagtggca ggttcagcgt tgtcagaccg ctggcaatgc
    35581 caagataaac aaacagttcg ccagggttga tatggagaaa gaggccattc attgagtggc
    35641 agctataaga ggctgccgcg tagtagctgg gtttgtactt ttccggcata aagcgaccca
    35701 ggcttaaggt catcggattg caaaagacaa acgtgccaat gcacggtagc agcaggtaac
    35761 gggaaacagg gttaccggcg caacgttgag caaaacgttc aatacgatgc tgaccgataa
    35821 aattaatcag tgcgttcatg ataaccagca ggctaatcaa cagcggaaga atgccggtca
    35881 ccatcccggt aaacacctct ccgccctttt ggaacagccc gataaaccac tctgcaccat
    35941 gagtaatggt ttctatcatt gttctctcct tcaggattta ttgttttatt accaaacggc
    36001 aacctaatct aatcagattg aaagatttga aagtgttatt ttgatcgcaa aatgaaagat
    36061 aaatatttta atttgaaagt ttgaataaaa ggatagcgag gggaatgagt tgagttatgt
    36121 aaagtccgta tcgggcagtg actaccgctt ccttgtgcgg ggcgtgatgc tttaccatac
    36181 ttgcccctgg ttgaatctgt taaatggacc cctcatgttc aagcgtcgtt atgtaacatt
    36241 gcttcccctt tttgtgttgc ttgccgcctg tagcagcaag ccaaaaccta ctgagactga
    36301 aacgaccacc ggaacgccgt ctggcggctt cctgcttgag cctcagcaca atgtgatgca
    36361 gatgggcggc gatttcgcta ataacccgaa tgcccagcag ttcatcgaca gaatggtgaa
    36421 caaacacggt ttcgatcgtc agcagttgca ggaaattctc tcccaggcga agcgtctgga
    36481 ttcggtactg cggctgatgg ataaccaggc accaaccaca tcggtgaaac cgccatcagg
    36541 tccgaacggc gcatggctgc gttatcgcaa aaaatttatt acgccggaca acgtgcagaa
    36601 cggtgtggtt ttctggaatc agtatgaaga tgcgttgaat cgcgcgtggc aggtgtatgg
    36661 cgtaccgccg gaaattatcg tcgggattat cggcgttgaa acccgctggg ggcgcgtgat
    36721 ggggaaaact cgcatcctcg atgcgctggc aacgctgtca tttaactacc cacgccgcgc
    36781 ggagtatttc tctggcgagc tggaaacctt cctgttgatg gcgcgcgacg agcaggacga
    36841 tccgctcaat ctgaaaggtt cctttgccgg ggcgatgggc tacggacagt ttatgccgtc
    36901 gtcttacaaa caatatgcgg tagatttcag cggcgacggg catatcaacc tgtgggatcc
    36961 ggttgatgcg atcggtagtg tggcgaacta tttcaaagcg cacggctggg tgaaaggcga
    37021 tcaggtcgcg gtaatggcaa acggtcaggc tccaggcttg ccaaatggct tcaaaaccag
    37081 gtacagcatt tcgcagcttg ccacggcagg tttaacgcca cagcagccgc tgggcaacca
    37141 tcaacaagcc agcctgctgc gtctggatgt cggtaccggt taccagtact ggtacggtct
    37201 gccgaacttc tacaccatta cccgttataa ccacagcacc cattacgcaa tggcggtctg
    37261 gcagttagga caagccgtgg cgctggcgcg cgtgcagtaa ctatttgcaa gaggggggcg
    37321 tgagcttccc tctggctaat cagctttttt ctgaatcctc ctcgtaaaat tgcaacgcca
    37381 acaccatctt cctgacgaaa gtgctatctt gtccggcata aatttttgac tgactgaggt
    37441 tgtgatgact gacagtgaac tgatgcagtt aagcgaacag gttgggcagg cgctgaaagc
    37501 ccgtggcgca accgtaacaa ctgccgagtc ttgtaccggt ggttgggtag cgaaagtgat
    37561 taccgatatt gccggtagct ccgcctggtt tgaacgcgga tttgtcacct acagtaacga
    37621 agccaaagcg cagatgatcg gcgtacgcga agagacgctg gcgcagcatg gcgcggtgag
    37681 tgaacccgtc gtggtggaaa tggcgatagg tgcactgaaa gcggctcgtg ctgattatgc
    37741 cgtgtctatt agtggtatcg ccgggccgga tggcggcagt gaagagaagc ctgtcggcac
    37801 cgtctggttt gcttttgcca ctgcccgcgg tgaaggcatt acccggcggg aatgcttcag
    37861 cggcgaccgt gatgcggtgc gtcgtcaggc tactgcgtat gcattgcaga ccttgtggca
    37921 acaatttcta caaaacactt gatactgtat gagtatacag tataattgct tcaacagaac
    37981 atattgacta tccggtatta cccggcatga caggagtaaa aatggctatc gacgaaaaca
    38041 aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt aaaggctcca
    38101 tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc ggttcgcttt
    38161 cactggatat cgcgcttggg gcaggtggtc tgccgatggg ccgtatcgtc gaaatctacg
    38221 gaccggaatc ttccggtaaa accacgctga cgctgcaggt gatcgccgca gcgcagcgtg
    38281 aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc tacgcacgta
    38341 aactgggcgt cgatatcgat aacctgctgt gctcccagcc ggacaccggc gagcaggcac
    38401 tggaaatctg tgacgccctg gcgcgttctg gcgcagtaga cgttatcgtc gttgactccg
    38461 tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct cacatgggcc
    38521 ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg aagcagtcca
    38581 acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg ttcggtaacc
    38641 cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt ctcgacatcc
    38701 gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc cgcgtgaaag
    38761 tggtgaagaa caaaatcgct gcaccgttta aacaggctga atttcagatc ctctacggcg
    38821 aaggtatcaa cttctacggc gaactggttg acctgggcgt aaaagagaag ctgatcgaga
    38881 aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa gcgaatgcaa
    38941 ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa gtacgtgagt
    39001 tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgacagc gaaggcgtag
    39061 cagaaactaa cgaagatttt taatcgttcg ttattgatga taatagataa tgtgtgcacg
    39121 gacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc caacactggc
    39181 gtgaaaaatt actctcacct cgatcctcta acgaattggc tgcacactca ttgactatta
    39241 aatctgttta tcagcaaaac ctgggtcgca tctgcggccc ttttgctttt ttaagttgta
    39301 aggatatgcc atgacagaat caacatcccg tcgcccggca tatgctcgcc tgttggatcg
    39361 tgcggtacgc attctggcgg tgcgcgatca cagtgagcaa gaactgcgac gtaaactcgc
    39421 ggcaccgatt atgggcaaaa atggcccaga agagattgat gctacggcag aagattacga
    39481 gcgcgttatt gcctggtgcc atgaacatgg ctatctcgat gacagccgat ttgttgcgcg
    39541 ctttatcgcc agccgtagcc gcaaaggtta tggacctgca cgtattcgtc aggaactgaa
    39601 tcagaaaggt atttcccgcg aagcgacaga aaaagcgatg cgtgaatgtg acatcgactg
    39661 gtgcgcactg gtgcgcgatc aggcgacgcg aaaatatggt gaacctttgc caactgtctt
    39721 ttcagaaaaa gttaagatcc agcgttttct gctctatcgt ggctatctga tggaagatat
    39781 ccaggatatt tggctaaatt ttgccgactg aacgcatacg gtattttact tcccactcaa
    39841 gaaaacttat cttattccca cttttcagtt accagcccgg cggttaagac acgctggagc
    39901 tggtggcgat atttcgttag cttgatttca ggataattat gagcaagagc accgctgaga
    39961 tccgtcaggc gtttctcgac tttttccata gtaagggaca tcaggtagtt gccagcagct
    40021 ccctggtacc ccataacgac ccaactttgt tgtttaccaa cgccgggatg aaccagttca
    40081 aggatgtgtt ccttggactc gacaagcgta attattcccg cgctaccact tcccaacgct
    40141 gcgtgcgtgc gggtggtaaa cacaacgacc tggaaaatgt cggttacacc gcgcgtcacc
    40201 ataccttctt cgaaatgctg ggcaacttca gcttcggcga ctatttcaaa cacgatgcca
    40261 ttcagtttgc atgggaactg ctgaccagcg aaaaatggtt tgccctgccg aaagagcgtc
    40321 tgtgggttac cgtctatgaa agcgacgacg aagcctacga aatctgggaa aaagaagtag
    40381 ggatcccgcg cgaacgtatt attcgcatcg gcgataacaa aggtgcgcca tacgcatctg
    40441 acaacttctg gcagatgggt gacactggtc cgtgcggccc gtgcaccgaa atcttctacg
    40501 atcacggcga ccacatttgg ggtggccctc cgggaagtcc ggaagaagac ggcgaccgct
    40561 acattgagat ctggaacatc gtcttcatgc agttcaaccg ccaggcagat ggcacgatgg
    40621 aaccgctgcc gaagccgtct gtagataccg gtatgggtct ggagcgtatt gctgcggtgc
    40681 tgcaacacgt taactctaac tatgacatcg acctgttccg cacgttgatc caggcggtag
    40741 cgaaagtcac tggcgcgacc gatctgagca ataaatcgct gcgcgtaatc gctgaccaca
    40801 ttcgttcttg tgcgttcctg atcgcggatg gcgtaatgcc gtccaatgaa aaccgtggtt
    40861 atgtactgcg tcgtatcatt cgtcgcgcag tgcgtcacgg caatatgctc ggcgcgaaag
    40921 aaaccttctt ctacaaactg gttggtccgc tgatcgacgt tatgggctct gcgggtgaag
    40981 acctgaaacg ccagcaggcg caggttgagc aggtgctgaa gactgaagaa gagcagtttg
    41041 cccgtactct ggagcgcggt ctggcgttgc tggatgaaga gctggcaaaa ctttctggtg
    41101 atacgctgga tggtgaaacg gctttccgtc tgtacgacac ctatggcttc ccggttgacc
    41161 tgacggctga tgtttgtcgt gagcgcaaca tcaaagttga cgaagctggt tttgaagcag
    41221 caatggaaga gcagcgtcgt cgcgcgcgcg aagccagcgg ctttggtgcc gattacaacg
    41281 caatgatccg tgttgatagt gcatctgaat ttaaaggcta tgaccatctg gaactgaacg
    41341 gcaaagtgac cgcgctgttt gttgatggta aagcggttga tgccatcaat gcaggccagg
    41401 aagctgtggt cgtgctggat caaacgccat tctatgcgga atccggcggt caggttggtg
    41461 ataaaggcga actgaaaggc gctaacttct ccttcgcggt ggaagatacg cagaaatacg
    41521 gccaggcgat tggtcacatc ggtaaacttg ctgcgggttc tctgaaagtg ggcgacgctg
    41581 tgcaggctga tgttgatgag gctcgtcgcg cccgtattcg tctgaatcac tccgcaacgc
    41641 acctgatgca cgctgcgctg cgccaggttc tggggactca tgtatcgcag aaaggttcac
    41701 tggttaacga caaggtgctg cgcttcgact tctcacacaa cgaagcgatg aaaccggaag
    41761 agattcgtgc ggtcgaagac ctggtgaacg cacagattcg tcgcaatttg ccgatcgaaa
    41821 ccaacatcat ggatctcgaa gcggcgaaag cgaaaggtgc gatggcgctg ttcggcgaga
    41881 agtatgatga gcgtgtacgc gtgctgagca tgggcgattt ctccaccgag ctgtgtggcg
    41941 gtactcacgc cagccgcact ggtgatattg gtctgttccg catcatctct gaatcgggta
    42001 ctgctgcagg cgttcgtcgt atcgaagcgg taaccggaga aggcgctatc accaccgttc
    42061 atgcagacag cgatcgctta agcgaagtcg cgcatctgct gaaaggcgat agcaataatc
    42121 tggcggataa agtgcgctca gtactggaac gtacgcgtca gttagaaaaa gaactacaac
    42181 agcttaaaga acaagctgcc gcacaggaga gcgcaaatct ttccagtaag gcaattgatg
    42241 ttaatggtgt taagctgttg gttagcgagc ttagcggtgt tgagccgaaa atgttgcgta
    42301 ccatggttga cgatttaaaa aatcagctgg ggtcgacaat tatcgtgctg gcaacggtag
    42361 ccgaaggtaa ggtttctctg attgcaggcg tatctaagga cgtcacagat cgtgtgaaag
    42421 caggggaact gattggtatg gtcgctcagc aggtgggcgg caagggtggt ggacgtcctg
    42481 acatggcgca agccggtggt acggatgctg cggccttacc tgcagcgtta gccagtgtga
    42541 aaggctgggt cagcgcgaaa ttgcaataat ataagcgtca ggcaatgccg tggactcgct
    42601 tcacggcatt cgcattaacg ctatcgacaa cgataaagtc aggttgaagt tgtgtatatc
    42661 ggctaaactt aggtttaaca gaatgtaatg ccatgactgc ttacatgtaa tgtgtttgtc
    42721 attgcttact ttttggcgtt atatgatgga taatgccggg atacagagag acccgactct
    42781 tttaatcttt caaggagcaa agaatgctga ttctgactcg tcgagttggt gagaccctca
    42841 tgattgggga tgaggtcacc gtgacagttt taggggtaaa gggcaaccag gtacgtattg
    42901 gcgtaaatgc cccgaaggaa gtttctgttc accgtgaaga gatctaccag cgtatccagg
    42961 ctgaaaaatc ccagcagtcc agttactaat ctttccgcgt ctcatcttta tcggtgagac
    43021 gcaccctcaa aatttctcct tcactctata gtcttttcgc tttactcccg ttcattcaac
    43081 ttaagtctcc atttttttgc attactgcta tctgccagac ctccgttttt ctgtagataa
    43141 aacactcttt ttgacgtttt tacagactaa ttgagcgtga agtgtgcaaa cgataaaagt
    43201 gtgggaaaaa ttgtttgact tataagtctc agaaagtaat atgtgcgcca cgcagcgacg
    43261 atgagcgata aacaagttct tcgaagcact cgtaagaggc gtgtggtgag gtggccgaga
    43321 ggctgaaggc gctcccctgc taagggagta tgcggtcaaa agctgcatcc ggggttcgaa
    43381 tccccgcctc accgccattt gcatccgtag ctcagctgga tagagtactc ggctacgaac
    43441 cgagcggtcg gaggttcgaa tcctcccgga tgcaccatct cttacttgat atggctttag
    43501 tagcggtatc aatatcagca gtaaaataaa ttttcccgat gcatccgtag ctcagctgga
    43561 tagagtactc ggctacgaac cgagcggtcg gaggttcgaa tcctcccgga tgcaccatat
    43621 tctccgaaat cttcagcaat gaaggtatcg aagaggtagc ggaattaacc gcaggcacca
    43681 gggatgataa cgttgcttta gcaacggccc gaagggcgag ccgcaaggcg agtaatcctc
    43741 ccggatgcac catctcttaa ttgatatggc tttagtagcg gtatcaatat cagcagtaga
    43801 ataagttttc ccgatgcatc cgtagctcag ctggatagag tactcggcta cgaaccgagc
    43861 ggtcggaggt tcgaatcctc ccggatgcac catattctcc gtaaccttca gcaatgaagg
    43921 tatcgaagag atagcggtat taaccgcagg caccaggaag gataacgttg cgccagcaac
    43981 ggcccgaagg gcgaacgtca gtgagtcatc ctcccggatg caccatcttc tccaaaaaat
    44041 ttccttcaaa aatatctcat ttctaaataa ctcctttatc catttcctga attttcttct
    44101 ccttcgccat cagcgacaaa atcaagcaat tacgctaaag tagcgtcata tttttcgact
    44161 tgcgagaaga ccatgtacga gcgttatgca ggtttaattt ttgatatgga tggcacaatc
    44221 ctggatacgg agcctacgca ccgtaaagcg tggcgcgaag tattagggca ctacggtctt
    44281 cagtacgata ttcaggcgat gattgcgctt aatggatcgc ccacctggcg tattgctcag
    44341 gcaattattg agctgaatca ggccgatctc gacccgcatg cgttagcgcg tgaaaaaaca
    44401 gaagcagtaa gaagtatgct gctggatagc gtcgaaccgc ttcctcttgt tgatgtggtg
    44461 aaaagttggc atggtcgtcg cccaatggct gtaggaacgg ggagtgaaag cgccatcgct
    44521 gaggcattgc tggcgcacct gggattacgc cattattttg acgccgtcgt cgctgccgat
    44581 cacgtcaaac accataaacc cgcgccagac acatttttgt tgtgcgcgca gcgtatgggc
    44641 gtgcaaccga cgcagtgtgt ggtctttgaa gatgccgatt tcggtattca ggcggcccgt
    44701 gccgcaggta tggacgctgt ggacgttcgc ttgctgtgag tgaagcgtta tcgcttttct
    44761 cgttgtttgc cagtagtttt ctcagcgcta cactattacc cggcaactcc gaagtcgtgc
    44821 tggtggcaat gttgctttcc gggatcagtc atccctgggt tttagtttta acagcaacaa
    44881 tgggtaatag ccttggaggg ttaactaacg ttatccttgg gcgtttcttt ccattgcgta
    44941 aaacatcgcg ctggcaagag aaagctatcg gctggctgaa acgctatggt gcagtcacgc
    45001 tattattaag ctggatgccc gtggttggcg atttactgtg tctgttagcg ggatggatgc
    45061 gcatctcgtg gggaccggta atcttttttt tgtgccttgg taaagcgtta cgctatgttg
    45121 cagttgcagc agcgaccgtt cagggcatga tgtggtggca ctaattgtag gcctgcacat
    45181 atggtcacca ttacagttat gctaattaaa acgattttga caggcgggag gtcaatttga
    45241 tcccggacgt atcacaggcg ctggcctggc tggaaaaaca tcctcaggcg ttaaagggga
    45301 tacagcgtgg gctggagcgc gaaactttgc gtgttaatgc tgatggcaca ctggcaacaa
    45361 caggtcatcc tgaagcatta ggttccgcac tgacgcacaa atggattact accgattttg
    45421 cggaagcatt gctggaattc attacaccag tggatggtga tattgaacat atgctgacct
    45481 ttatgcgcga tctgcatcgt tatacggcgc gcaatatggg cgatgagcgg atgtggccgt
    45541 taagtatgcc atgctacatc gcagaaggtc aggacatcga actggcacag tacggcactt
    45601 ctaacaccgg acgctttaaa acgctgtatc gtgaagggct gaaaaatcgc tacggcgcgc
    45661 tgatgcaaac catttccggc gtgcactaca atttctcttt gccaatggca ttctggcaag
    45721 cgaagtgcgg tgatatctcg ggcgctgatg ccaaagagaa aatttctgcg ggctatttcc
    45781 gcgttatccg caattactat cgtttcggtt gggtcattcc ttatctgttt ggtgcatctc
    45841 cggcgatttg ttcttctttc ctgcaaggaa aaccaacgtc gctgccgttt gagaaaaccg
    45901 agtgcggtat gtattacctg ccgtatgcga cctctcttcg tttgagcgat ctcggctata
    45961 ccaataaatc gcaaagcaat cttggtatta ccttcaacga tctttacgag tacgtagcgg
    46021 gccttaaaca ggcaatcaaa acgccatcgg aagagtacgc gaagattggt attgagaaag
    46081 acggtaagag gctgcaaatc aatagcaacg tgctgcagat tgaaaacgaa ctgtatgcgc
    46141 cgattcgtcc aaaacgcgtt acccgcagcg gcgagtcacc ttctgatgcg ctgttacgtg
    46201 gcggcattga atatattgaa gtgcgttcgc tggacatcaa cccgttctcg ccgattggtg
    46261 tagatgaaca gcaggtgcga ttcctcgacc tgtttatggt ctggtgtgcg ctggctgatg
    46321 caccggaaat gagcagtagc gaacttgcct gtacgcgcgt taactggaac cgggtgatcc
    46381 ttgaaggtcg caaaccgggc ctgacgctgg gtatcggctg cgaaaccgcg cagttcccgt
    46441 tactgcaggt gggtaaagat ctgttccgcg atctgaaacg cgtcgcgcaa acgctggata
    46501 gcattaacgg cggcgacgcg tatcagaaag tgtgtgatga actggttgcc tgcttcgata
    46561 atcccgatct gactttctct gcccgtatct taaggtctat gattgatact ggtattggcg
    46621 gaacaggcaa agcgtttgca gaagcctacc gtaatctgct gcgtgaagag ccgctggaaa
    46681 ttctgcgcga agaggatttt gtagccgagc gcgaggcatc tgaacgccgt cagcaggaaa
    46741 tggaaaccgc tgataccgaa ccgtttgcgg tgtggctgga aaaacacacc tgacagaaaa
    46801 gaaaaaggcc actcgtgagt ggccaaaatt tcatctctga attcagggat gatgataaca
    46861 aatgcgcgtc tttcatatac tcagactcgc ctgggaagaa agagttcaga aaatttttaa
    46921 aaaaattacc ggaggtggct aaatgccgtt gttagatagc ttcacagtcg atcatacccg
    46981 gatggaagcg cctgcagttc gggtggcgaa aacaatgaac accccgcatg gcgacgcaat
    47041 caccgtgttc gatctgcgct tctgcgtgcc gaacaaagaa gtgatgccag aaagagggat
    47101 ccataccctg gagcacctgt ttgctggttt tatgcgtaac catcttaacg gtaatggtgt
    47161 agagattatc gatatctcgc caatgggctg ccgcaccggt ttttatatga gtctgattgg
    47221 tacgccagat gagcagcgtg ttgctgatgc ctggaaagcg gcaatggaag acgtgctgaa
    47281 agtgcaggat cagaatcaga tcccggaact gaacgtctac cagtgtggca cttaccagat
    47341 gcactcgttg caggaagcgc aggatattgc gcgtagcatt ctggaacgtg acgtacgcat
    47401 caacagcaac gaagaactgg cactgccgaa agagaagttg caggaactgc acatctagtc
    47461 agtaaactat cttcacaatt aattgaaaaa agccagttca aatgaactgg cttagttgta
    47521 cttagtgcgc accgccgcca ccgccacctg cgccaaatgg cggtttagca aaccacacca
    47581 gccccagcag tacgaggaat atcccggctg acatccagaa gatctcattg gcggaaataa
    47641 tcagcccctg attggtgatc tgctgggcaa tccagcctga tgcctgctgt tgcgtcatcc
    47701 caagcccttc cagttgactg tacatcgcct gggcattcgg gttgaacggg tttaccgact
    47761 cagtcaactg cgcatggtgc atcgactcgc ggttggtcca catggtcgtg gttatcgacg
    47821 tgccgataga ccccgccagc gttcgcgtaa agttagagag gctcgatgcc gccgccagtc
    47881 gttccggtgg caaaccagac agcgtaatgg tggtcagcgg cataaagaag caggccaccg
    47941 caaacccctg gataaactgc ggccaggccg acgcgccaaa atccatacct ggttcaaagg
    48001 tataggcacg ccagtagaag cagacggcat acataataaa gctgaaggtt accagccgcc
    48061 gcatatccag tttatgcgcg aagcggccga taatcggcga caggatcacc ggaataatcc
    48121 ctaccggcgc agaggccaaa cctgcccagg tcgccgtgta accgtagacc tcctgcaaca
    48181 actgcggcag cagaacaata gcgccgaagt agagcatata cgcgaggctg atacacaagc
    48241 agccgatggt gaagttgcgc gacttaaaca acgacagatc gactatcggg ttatcgtcgg
    48301 tcagctccca gacaatcagg aagcagatag ccaccaccgc caccacggta aggatgataa
    48361 tttcctgtga tgaaaaccag tccagctctt taccgcggtc gagcataatc tgcaggctgc
    48421 cgataccaat aaccagcagt gccagcccca cggcatcaat ccgccgccgt ttggtgcggg
    48481 tttcacgtcc gcgcagagtt tgcagtgtca tcaacaccac cgccacgcca atcggcacgt
    48541 tgatgaagaa tatccagccc cagtggtaat tatcgctgat ataaccgccg aggatcgggc
    48601 cgcaaattgg cgcgacaatc accgtcatcg accacaacgc cagcgcgatc gagcgtttgg
    48661 ctggcgggta gttattcagc aatagacttt gcgaaagcgg gatcaacggc ccggcgacaa
    48721 tcccctgaat cacgcggaag aagatcagca tattcaggct gctggagaca ccacacgccc
    48781 acgacgcaat agcaaaggcg atggtggacc aaaggaacag tttcacttcc ccgacgcgct
    48841 ttgccagcca gccggtaagc gggatcgaga tggcattcgc caccccgaaa gaagtgatta
    48901 cccacgttcc ctggctgagc gatgagccca gattcccggc gatagtgggg atcgccacgt
    48961 tagcaatggt ggagtccagc acctgcatga atgtcgccag tgacagcgca atcgtcataa
    49021 tgaccagttg cgcgccttcc agcggttttt gctgttgcat cacacgcacc tctggattag
    49081 ccagcgttag cttttacgat atcgtcgatc agtttattga caggtgccag gctgatttca
    49141 cgcgcggtac ttaccgcgac cggtgttgaa cgtactttat ttgccagtac ctgaccgtca
    49201 cggttagtgg tattgacgct caccagtgtg gacaaaccaa tgcgcagcgg gtattgctcc
    49261 agctgtttct ggtccagttc gatacgcaca ggtagacgct gaacgacttt gatccagtta
    49321 ccggtcgcat tttgcgccgg aagcagtgag aatgcgctac ctgtgcccat atccagacca
    49381 accactttac cggtgtattt cacatcatcg ccgtaaatat ccgtggtgat agtgaccggc
    49441 tgaccgatac gcatattggc aatctgcgtc tctttaaagt tggcatccac ccacatattg
    49501 gtggctggaa cgaccgccat cagcggcgtc gttgggctaa tttgcgcccc aggctgtacc
    49561 gcacggcggg agacataacc ggtcatcgga ctgacaatac gggtacgctc cagcgccagc
    49621 caggcgttac gtacttcggt ggcagcctgt tgcacggcag gctgatcttc cagtttagtc
    49681 cccagaatca tcgcctgatt ggcattgtat tgttgaatag cgacgtccag ttgcgcctgg
    49741 gcactggtga cggcgtcgcg ggcgtgttgc agctcttcgc gaccaatcag gttggcattg
    49801 cccagcggca cacggcggtt gtagtcgctt tgtgcttttg cgagggcgat tttctgcacc
    49861 tcaatattcg cctgcaactg tttgctgtta atcatcagct ggtgggtttg gcgaacgctg
    49921 gaagccagtg cagttttggc tttttcaaac gcctggcgag catctgtcgg gtcgagagtg
    49981 accagcacgt cgccttcttt tacaaaatcg gtgttatcgg cccagacttt cgtcacgctg
    50041 ccagacacct gagacataat ttgaatctga ttccctgcca cgtatgcgtc atcggtttct
    50101 tcgaaatgac gcagtaccaa aaaccaataa atccctatcg ctacggcaat aattataaag
    50161 agcaaggtga gaaggaggag cagacgctta cgtttgccgc tcttctttac cggttgctgc
    50221 ggggtttgag tctccgcatt tgcgctcata ttgttctcca cgatcttctt aactcatcgg
    50281 ctgagccgac ctgcttgcca aaaaggccag catgttgcga tgctggccgg tcattttttc
    50341 ttttataaat ctggattttt gagcgagatg acgcgttagc tcatcgcttc gagaaccaca
    50401 ccgtcttgtt ccatctggtc gagacgggag agcaatttgc gggtgatttg ctcgagctga
    50461 tctttttctg ttgtgctgag cgcggaccag agttgatgca ggcagttatg ctgcggtggt
    50521 aaaacctcgc gcaaaaactc gtgacctttt tccgttaatt gcagatgcag gcagcggcga
    50581 tcgttatcgc tttcacgacg ttcgatccaa ccgcgttttt ccagttcatc ggcaatacgc
    50641 gtcgcgttgg tacgggatga tccaagagca caacttaatt cagaaggctg aatactgtgg
    50701 ttttcctgag actccagcgt aatcaacgcc ataaacaacg tctcgttaat cccctgagcc
    50761 ttcagcattt tattgcggtt ctccagcagc ttgctttgca tgtgcatgca aagacgagtc
    50821 agaaggatct cctgataagg aaaatcttcg tggcggctgg cgcgaaattt tagcatttgt
    50881 tcaatgggcg taaacgaact atccatttgg gtatgacctc attaatttca gccgatatag
    50941 taacgacagt gacaaataaa gtaaatgtat tgttttagaa aaatgattct tgtgggttat
    51001 ataatcgcca tcactttcca ggcgagccca taggccagcg cactaagcag tgttgggatg
    51061 ataatgctgc gtgttttata gaaactggca cccagtaccg cgaagccgac cagcgtgggc
    51121 acgaaacggc gtgtatcgtg catcacttct ggtgcggtag agacaaccag cagagcgcat
    51181 atcgaggcga tgccaatggt gtcgagcaaa atacctaccg cgccacgttt ggttgggcgg
    51241 gcattaccca cacgcaggcg cagcggcaaa tagcggaagc aataattcgc cgcgccaacc
    51301 agtaacccag gcagcagaac ctcatagctc atcgggcgct ccttgccaga atgcttggat
    51361 taacgcagtg aggcagccac agacaatgcc tgccagaatg gcgacgggaa tagaaaatag
    51421 cgttacgcct gcaagggcac caactaacgc tgcggtaacg caaagagatt gtttgcgctg
    51481 gaaagaggcg agcaggaaac tcataaagag tgccggaagc ataaaaccta atgctgcttc
    51541 aacggcggga taaccttgca gcaagccgct gccagagaat gcccctatta ccgtaccaaa
    51601 tacccacgat gaccatgaac tgaaggcaat gccgatcatc cagttctcgc tccagcggcg
    51661 attattgcgt accagttttg ctgttgcggc ggcaaaaacc tcatccgtca ggccaaacgc
    51721 ccacagggcg gttttcgatt tttgcagacg ctgaataata cggctacgca gtgacgggcc
    51781 atacaacaca tggcgaacat ccattgccat gacggtcagt gcagcaaccc acaaactact
    51841 cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg cctgcataaa tgatgcagga
    51901 gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc gcattcagac cgaacgcaaa
    51961 ggccaccgga atataactaa taacaatcgg taaactgtct ttgcatcctt ccatgaaggt
    52021 cgccgaacca ggagcaggct gtggagtagg gctttccata gagtgtacgc ttaacgattg
    52081 ttaatattat aaatagactg aatgaatatc ttaaccttat cagactgatg ggcttcttaa
    52141 cacccttata agtgtaaagc cacgaaaacg gttgctgatt gcttaatttg cggcttcctg
    52201 acgatgaaaa cctcgccacc agaccagtaa gttaactagg gcaatcgtcg cgccagccag
    52261 acaaacgcca gcccaaccgc catgttgcca ggctgaggct gaaattagcg aaccggcggc
    52321 accgccaata aagtagctgg tcatgtaacc tgcggtcagg cgattacgcg catcagggtg
    52381 tattcgataa attaccgtct ggttagtgat atgcacgccc tgtacggtga gatccagcac
    52441 caggatgccg ataatcagcg ccagtacgga agtgtgacca aaccagatcg ccagccatga
    52501 aagtaatagc agcagcagac cgaaagttgt ggtgtggtgc gatttaccct tatcggcaaa
    52561 accgcccgcc ggacgagcgc ccaacgctcc ggcagctccc gcaagtccaa acagaccaat
    52621 gacaccatcg ctgtagttaa aaggtggagc ggcaagcaaa aaggccattg aggtccagag
    52681 aatgctgaag ttggcaaagg tcaggcagcc cagcaatgcg cgggtgcgga ggatcttatc
    52741 gctgataaac atgctgaaaa cggaacctaa caactgtggg tagttgaggt gggtttctga
    52801 tttcatttgt ggcagaccac gccataatgc tagcgccatc agtgccatta acaccgaggc
    52861 aacccaaaag acggtgcgcc agccgccgag gtttgccagt aacccggcaa cggttcgtgc
    52921 cagcaagatc cccaacagca gaccgctcat aatggtgcca accactttgc cgcgtttatc
    52981 cggtgaagcc agcgtcgctg ccagcggaac cagaatttgt gccacgactg agaataaacc
    53041 ggttaatgcc gtaccgagga tcatcatcgc cagcgactga ctgctggcag tgattaacat
    53101 gccgcctgcc gccagtaagg tcatcgagac aatcaggcgg cggcgttcaa acatatcacc
    53161 gaggggaaca agaaacagca gacctgctgc atagcccaac tgcgcggcgg taacaataaa
    53221 gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg gtgtcgagca atggctgggc
    53281 gtaatagtta ctggctaccg cctgaccggt ggcgatagac atcagcacga tcagcgccgg
    53341 gctaagctca tgattaggtt tagtcattat ttttgttcat caggtgatga ttaagctgga
    53401 aatcaataat agcggatgtt gatgaatggg ggcgaaagat agcttgttga aatgtgcgat
    53461 gtggtttgta ggcatgataa gacgcgccag cgtcgcatca ggcattgtgc acagaacggc
    53521 ggatgcggcg aaaacgcctt atccgccata cggaatgagt gtattttgta ggcatgataa
    53581 gacgcgccag cgtcgcatca ggcattgtgc acagaatggc ggatgaggca aaaacgactt
    53641 atccgcccga ataaaaatta cttctgcgct gccagcgcct cattcaccca gccatcgaac
    53701 tgctgctggt gggctttgat ccaaccatca acatggccct gaatatcgcc ttctgaggct
    53761 ttgccgtcat gcataatggc gttctgggcg ttaatatctg ccactggcaa ctgcataatg
    53821 gcaaaaagtt tcgctgctgc cgggtttttc tcggcccagg ctttgttggc aacgatatgc
    53881 atggtgctga ccgggaagcc ataattcgca ccattcggca gtttggtatc ggcgttttta
    53941 tcgcccggca gtgcggagaa cggcacctgc aaccagacca catctttccc tggcttcagc
    54001 tcattactca cccagtacgg cgtccaggtg taataaaaca ccggtttgcc ctctttgtag
    54061 cgactgatgg tgtcggccat catcgctgcg tagttcccct gattatgcgt cacggtgttg
    54121 gtcagttcat acgcggcaag ctggtggttg atcgcacctt cgcagcccca gcctgggtta
    54181 cagccggtta aatccgcttt accgtcgccg ttggtatcga acagtttggc gatcttcgga
    54241 tctttcagtt gtgcgatgtt ggtgattttg tactggtcgg cggttttctt atcgatcagg
    54301 taaccctgtg ccgcgccgtt aacgaatatc ccttcacaat aaaatttctt atcgccaccg
    54361 gcagcttcgt acatgttgtc atgcagcggc gtccagttca cggcggtgaa ggttgcatcg
    54421 ccggaagcaa gtgaggtgta gccaacgttg taatccactt cgctgggttt attgacggta
    54481 taacctaatt tctccagtgc acgactgacc agcagcgtct ggaaggtttc ttcagtgatg
    54541 gtgctctgaa ctggattaac agtaatgcct ttgcccggca gatcggctgc aaaagtttgt
    54601 gtagagataa gcgtggcaaa cgctgtcgca aaaagtacgc tatgtcgcat tgttattcct
    54661 ttttttggca gggtgataat gcccggcgtt accgggcaag tgcagagtta cttaatgaat
    54721 gggcgggtca gcagaccgac agggccagtg gtgtaccagc gacggttgcc gcgactgcgt
    54781 gagtcgcgcc caacggcctg cgtcaggcgg tcgaggataa tggcgaggat cacaatcccg
    54841 acgccgccaa cggtggcaag gcccatatcc agacgaccga taccgcgaag taccatctga
    54901 cccagcccgc cgacggcaat catcgaggcg atgaccacca tagaaagagc cagcatcagc
    54961 gtctggttaa cgcccgccat aatggtcggc atcgccagcg gtaactgaac tttgaacagc
    55021 atctggcgcg ggctggcacc gaatgagcgc gaggcttcaa tcagatccgc cggaacctgg
    55081 ttaatcccca gaatcgtcag acggataatc ggcggcagag caaagatgat cgtcaccact
    55141 acgcccggca cgttaccgat accaaacagc atgacgattg gtaccagata aacaaacgct
    55201 ggcgtggtct gcatggcatc aagcagtgga cgaataattt tcgccgctcg cggacttctc
    55261 gccagccata tccccaacgg caaaccgatg acgatacaga acagcagggc ggttaacacc
    55321 agcgccagag tcaccattgc ctgcgaccag gcaccgattg cgccgatggc aatcagcgaa
    55381 accagcgtcg ccacgcccat tccgaccccg gaaatctgcc aggcgatgag agcgaaaacg
    55441 ataatcgcca ctggcgctgg catacccagc agcaattgct ggaaactgtt gaggatataa
    55501 tcaaccggaa cgcgcacgcc ctggaagacg ggacggaaat gggtaacaac ccagtcgatc
    55561 ccttcagtga cccaactgtc gagcgggatc agcgttttat ggaacggatc gagaatatta
    55621 aaatgctcga cgtttggcgc aggcgtactg gtcagccagt cagcaccacc gccgtcagtc
    55681 ggtgcagtcg tcggtgtacc ccaggcgtct gcggattgcg cggcactgtc cgccgctggc
    55741 gtggtatccc acggattatt ttgatcagcc attatttacc ccctcacgat ctaaagcgcg
    55801 cagcagcatt cctttcgaaa tgatgccgac atactgttgg tcctcgtcga ccacgggcac
    55861 cgcacagggt gcctgtccga catgagagag caactcgcta agaggcgttt gtgcatcgac
    55921 tgctaacggc gcatcaatca gcgccgcatc aagaccttgc tgctgcgtta acgcggtttt
    55981 aagcgaatcg atggagactg cgccgacaaa cttattaccg cgttcgataa cgtagccata
    56041 ttcgcgatct tcatcctgca ataatttcag tgccgaacgt gggccgaagc caggggtttt
    56101 acgaattaag ccattcggtg tccggcgggc aatatctttc gcactgaata cctgactaat
    56161 atcaacgcca cggaagaagg tacggacata atcattcgcc ggattattga gaatttcatc
    56221 cggtgtgccg acctgtacca cttcaccatt ttgcataatg gcaattcggt cgccaatacg
    56281 catggcttca tcgagatcgt gggaaataaa gacaatggtg cgctgatgtt tcgcctgtaa
    56341 ttttaccagc tcatcctgca tctcggtgcg aattaatgga tcgagcgccg agaaggcttc
    56401 gtccattaat aaaatatccg gattaatcgc taacgcgcgg gctaatccca cacgttgacg
    56461 catcccgcca gagagttcat ccgggtagct gtgggcataa ttttccagcc cgacctgacg
    56521 cagtgcatca agggcttttt cccggcgttc ttcggcatta attccggcca attccatacc
    56581 gaacgcagta ttgtccagca cggtcatatg cggcattaag gcaaaggact ggaagaccat
    56641 cgcaatcttt tttctgcgca cctcacggag tttggcatca gatattttgg caatatccac
    56701 accatcaatc agcacttgcc cgcgggtggg ttcaatcagg cgattgagaa ggcgtaccat
    56761 tgtggattta cccgagccgg ataatcccat gatgacaaat atctcgcctt cttcaatggc
    56821 cagactggcg tcttttacgc caagcgatag cccagttttt tccagaattt gttcttttga
    56881 aagtccttgt tcgatatatt tgaatgctcg ctgtggatgc tcaccaaata ttttataaag
    56941 gtttttaatt tctaatttaa ttgccatgca atagaaagat tcctgttatt tgtttatgtc
    57001 gatatattac ccaatggaaa tagttacttt tttctaccct aacatactga gaatctgagg
    57061 caacccctga tggcaaatgt ggcatgaata ttgatgcgag caaatcgccg gaatttcccg
    57121 tgatataagg gctgagagca aatcgagaaa agttgatatt ttttgtcaaa gatagcgaaa
    57181 aaacgcctga atattgtggt ttcaggcgaa taatgcaaca gagtatcagt gtagatcacc
    57241 acaaaatatt tgcgtgataa aaagctattt ggcgggaatt atttccctgt cgcgggtagt
    57301 gatatttttg aaaataacac cctcagaaat tccagtcttc atcttctgtt tcaaccgctt
    57361 tccccatcac ataagaggag cctgaaccgg aaaaaaaatc gtgattttca tcggcattcg
    57421 gcgaaagcgc ggcgaggatt gccggattca cttccgccat ttctgcggga aataaaggtt
    57481 cgtagcccag attcatcaaa gccttattgg cgttgtaaca gagaaacgct ttcacatcgt
    57541 cagcccacgg ggtttcggcg tacagctcat cggtgtattg cagctcgttg tcgtagagtt
    57601 ccagcaacaa atcgaaggcg aaactcttca actcttcacg ttgtcccaga gatatctttt
    57661 ccatgttttt ctgatattta tagcctatgt agtaaccgtg gactgcttca tcacggataa
    57721 tcagacggat caggtctgcg gtattggtca gctttccgcg gctggaaaaa tacatcggca
    57781 gccagaaacc ggaatagaac aaaaaagatt caagaaacac actggcgatt ttctttttca
    57841 gcggatcatc accgcgataa tgttgctgaa taatctgagc ttttcgctgc aacggtgcgt
    57901 tttcttcact ccaggcgtag gcggcatcga catctttggt ctggcacagc gtcgagaaaa
    57961 tcgaactgta agagcgggca tgaaccgctt ccataaagct gatattcgat aataccgctt
    58021 cttcatgagg cgtgagtgca tcgggcatca gcgaaggcgc gccgataaca ttttgcagcg
    58081 tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag ttgttgttct acgacagtta
    58141 atgtctgcca ggcaggaata tcgttcgaca gcggcacctt ttctggtagc cagaaattgc
    58201 tggtcaggcg attccacacc tccagatctt tatcgtcaga tatcttgttc cagttgatgg
    58261 cgctgatacg tgagagtttc atagatattc cttaaagtgc acaggagacg cagccttcaa
    58321 tttcagtgcc ttccagcgcc atctgacgca ggcgaatgta atagagcgtt ttgatacctt
    58381 tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt ggtggcggta tcggggaaaa
    58441 acagcgtcag cgacaaaccc tgatcgacat ggcgagtcgc ttccgcgtag gtgtcgatga
    58501 tcttttctgc gccgatttcg taagcatcct gatacagcgc cagattttcg ttagtcataa
    58561 acggggcagg gtagtaaacg cgtcctgttt tgccctcttt gcgtatctct actttcgcca
    58621 caatcggatg aatactcgac gtagcatggt tgatataaga gatagaaccg gttggcggca
    58681 ccgcctgaag attctggttg taaatgccgt agcgcatcac gtcgtcgcgc agttgcgccc
    58741 acatctcacg ggtaggtaac gtaataccgc tgcgggcaaa cagttcgcca actttcgccg
    58801 ttttcggctg ccagttacct tgcagatatt ggctaaaata ttcaccactg gcataacgtg
    58861 actgtttgaa cccggcgaag gtttcaccgc gttcgcgcgc cagcaacatc gaggtacgca
    58921 gtgcgtgcca ggtgatggca tagaaataga gattggtgaa atccagtgct tccggcgaac
    58981 cataagcgat gccttctcgc gccagatagc cgtgtaaatt catctgcccc agtccgatgg
    59041 cgtgtgaggc ggcatttccg gcttcgatgg acggcacgct gcggatatga ctcatatctg
    59101 acaccgccgt taaaccgcgc acggcagtct ctaccgtgcg ggcaaaatcg ggggaatcca
    59161 tggtgtgcgc aatattcaac gaacctaaat tgcaggagat atcatggcct gtgcgggcat
    59221 agtcgagatt ctcgtcatac tctgaggcac agttgacctg caaaatctct gagcagagat
    59281 tactcatatt tatgcgcccg gcgacagggt tagcacggtt taccgtgtct tcatacatga
    59341 tgtagggata gccggactca aactggattt ctgccagccg ctggaagaaa tcacgggcgt
    59401 tgaggtattt tttgcggatg cgttcatcgg caaccagttc gtcatagtgt tcgctgatgg
    59461 cgatatcggc aaacggcttg ccataaactc gctcaacgtc atagggcgaa aacagcgcca
    59521 tctgcgcatt ctcttttgcc agatggaaag tgatatccgg gatcaccacg cccagcgaca
    59581 gtgttttaat gcggattttt tcgtcggcat tttcccgttt tgtatcgaga aaacgcagaa
    59641 tatcgggatg atgagcatgt aaatagactg cacctgcccc ctgacgcgcg ccgagttggt
    59701 tggcatagga aaatgcgtct tccagcattt tcatcaccgg aattacgcca gaagattgat
    59761 tttcaatacg tttaattggc gcgcccgctt ctcgcagatt cgacagcaaa aatgccacgc
    59821 cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc ccggccaatc gactccatat
    59881 tgtcttcaat acgcagcaga aaacaggaaa ccagttcgcc gcgctgctgc ttaccgcagt
    59941 tgaggaatgt cggcgtggct ggctgaaagc gtcctgacag catttcattg gtcagttgca
    60001 acgccagcgt cgcatcgcct tgtgccagcg tcagcgccac catcgtgact cgatcggcaa
    60061 aatcttccag ataacgttta ccgtcgaatg tcttcaacgt atagctggtg taaaacttcc
    60121 atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg tgcgtgggca aacagcgtaa
    60181 tgacaaagtc gcgagagtag cgattaagaa cgctttcatc atagtaacct tcgttgacca
    60241 gccagttcag gcgctgctgc tggctactga aggtcacact gttcggacgc acatgcgtcg
    60301 ccataaaggc gtcaacggcc tggcggtctt tatcgaactg aatgcgacct gcgctatcgt
    60361 agaggttaag catcgcattc agcgcgtggt aatccatcgt ttcctgcgtc aggcattctg
    60421 cggttgtcgt tgccaaaatt cggttactcc tttacgaacg ttttcgatat cgctttgcgt
    60481 acccatgagt tcaaaacggt acagccacgg cacgccgcat ttccgggcaa tcacatctcc
    60541 ggcgcggcca tacgcctcac caaagttgcg attaccagaa gcaataacgc cgcgaagcaa
    60601 cgcccggttg tgctcgtcgt ttaaaaagcg aattacctat cgtggcaccg cgccagccgt
    60661 accgccgccg ccgtaagagg gcacgatcag gatgtaaggc tcgtctacct gaatccgttc
    60721 ccgctcattg agcgggatgc gcaccgcggg cagacctaaa cgttcgataa aacgctgcgt
    60781 gttttcggag ctgctggaga agtagacgag ctggctcatg cactggccgc gtgtggcgct
    60841 ggatgcagac ggttaatcat gtccggacgg aaaccagacc agctaagatc gccagcaatc
    60901 actaccggca actgacgaaa gccctgagca cgcaacgctt ccgctgcttc aggaacgcga
    60961 tcgacattaa tcatttcaaa atcaaagccc cggttttcca tcgcccgttt ggtggcgtgg
    61021 cactgaacgc aatcgttacg agtgtaaata gttatgcgca tgattcgtat ttccgtttaa
    61081 aatgaagata cggcgcgatg atacgcgtcg ggttgtctct ctgttgatac agagatacta
    61141 gatgtagttg aaaaaagatt caaccacaca atatatagca aatagtgatc gaaattaccc
    61201 tagatatgag cgtgacgggg gagggggatt tttgtgattc accaggcaaa aagaaacccc
    61261 gaagacaggc ttcggggtta aagacgcgta tttattatca tttttgcact acgatttgcg
    61321 catgcttaac agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca
    61381 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgtgcgg cttgctggac
    61441 gcgagtacga ccatgcattc gcgccctggt ttctttcagc aatgcctgag ctttactgcg
    61501 tgcggcttca gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgaggct
    61561 gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
    61621 gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc
    61681 ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag cgttgtgaat
    61741 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
    61801 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
    61861 gaaagcttat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
    61921 gggacgtacc gcactggtaa ttcatccaac attaaaagaa cgtagttcga cactggctga
    61981 acccgcttcc gatattaaaa cttgcgatca ttatcaacaa tttccgctct atttagcagg
    62041 cgagcgacac gagcattacg gtatcccgca tggcttcagt tcgcgtgttg cgcttgaacg
    62101 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
    62161 aaaagaagag tcaggctttt acctgctggt aatggctgac tttaaacagt cggcggcaat
    62221 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
    62281 ccgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
    62341 gtgactggaa cgtcacataa gccaggatat cagccagatt ttttatccag cccgacgggc
    62401 taactttgcg tgccgcgcgc ataaatagat cgcggtacat accgtatggc catgcaatta
    62461 agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
    62521 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
    62581 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
    62641 gcgtaatggg atgttaatgg agatgaaagc agtggatctc attgccatct gcattttcgc
    62701 atagcgtata tcgctggcta tgactattca attagtgaaa aatttttatt tagtcagttt
    62761 ttgtagataa aatattccgt aacggctcgt tttttgtaca ttgtggattt ttacatgctg
    62821 atatgattta ttttcaatta aatcaattag atatgacctt gcctttaatt tcactaatga
    62881 aagtttgggg gtgaggtaac gctataagcg tagagaattc ggtgtatgtc gtgaccgtgt
    62941 catttacagc atttttgatg ccagaaaatg tacattaaca gcagaaagca ccagtg
//
LOCUS       DAZOXR010000004        58931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZOXR010000004 DAZOXR010000000
VERSION     DAZOXR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.004"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(185..1132)
                     /gene="dusC"
                     /locus_tag="ACHUNN_000202"
     CDS             complement(185..1132)
                     /gene="dusC"
                     /locus_tag="ACHUNN_000202"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0574696.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1371..1769
                     /locus_tag="ACHUNN_000203"
     CDS             1371..1769
                     /locus_tag="ACHUNN_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0574697.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1766..2461
                     /locus_tag="ACHUNN_000204"
     CDS             1766..2461
                     /locus_tag="ACHUNN_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0574698.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2591..3475
                     /gene="cdd"
                     /locus_tag="ACHUNN_000205"
     CDS             2591..3475
                     /gene="cdd"
                     /locus_tag="ACHUNN_000205"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0574699.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3625..4344
                     /gene="sanA"
                     /locus_tag="ACHUNN_000206"
     CDS             3625..4344
                     /gene="sanA"
                     /locus_tag="ACHUNN_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0574700.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4347..4586
                     /locus_tag="ACHUNN_000207"
     CDS             4347..4586
                     /locus_tag="ACHUNN_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0574701.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4780..6018
                     /gene="preT"
                     /locus_tag="ACHUNN_000208"
                     /pseudo
     CDS             4780..6018
                     /gene="preT"
                     /locus_tag="ACHUNN_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            6012..7247
                     /gene="preA"
                     /locus_tag="ACHUNN_000209"
     CDS             6012..7247
                     /gene="preA"
                     /locus_tag="ACHUNN_000209"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR0574702.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7374..8384)
                     /gene="mglC"
                     /locus_tag="ACHUNN_000210"
     CDS             complement(7374..8384)
                     /gene="mglC"
                     /locus_tag="ACHUNN_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0574703.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8400..9920)
                     /gene="mglA"
                     /locus_tag="ACHUNN_000211"
     CDS             complement(8400..9920)
                     /gene="mglA"
                     /locus_tag="ACHUNN_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0574704.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9981..10979)
                     /gene="mglB"
                     /locus_tag="ACHUNN_000212"
     CDS             complement(9981..10979)
                     /gene="mglB"
                     /locus_tag="ACHUNN_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0574705.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11259..12257)
                     /gene="galS"
                     /locus_tag="ACHUNN_000213"
     CDS             complement(11259..12257)
                     /gene="galS"
                     /locus_tag="ACHUNN_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0574706.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12441..13598)
                     /gene="yeiB"
                     /locus_tag="ACHUNN_000214"
     CDS             complement(12441..13598)
                     /gene="yeiB"
                     /locus_tag="ACHUNN_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0574707.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13615..14283)
                     /gene="folE"
                     /locus_tag="ACHUNN_000215"
     CDS             complement(13615..14283)
                     /gene="folE"
                     /locus_tag="ACHUNN_000215"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0574708.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14541..15377
                     /gene="yeiG"
                     /locus_tag="ACHUNN_000216"
     CDS             14541..15377
                     /gene="yeiG"
                     /locus_tag="ACHUNN_000216"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0574709.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15409..17400)
                     /gene="cirA"
                     /locus_tag="ACHUNN_000217"
     CDS             complement(15409..17400)
                     /gene="cirA"
                     /locus_tag="ACHUNN_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR0574710.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17692..19161)
                     /gene="lysP"
                     /locus_tag="ACHUNN_000218"
     CDS             complement(17692..19161)
                     /gene="lysP"
                     /locus_tag="ACHUNN_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0574711.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19366..20247)
                     /gene="yieE"
                     /locus_tag="ACHUNN_000219"
     CDS             complement(19366..20247)
                     /gene="yieE"
                     /locus_tag="ACHUNN_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0574712.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20346..21395
                     /locus_tag="ACHUNN_000220"
     CDS             20346..21395
                     /locus_tag="ACHUNN_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0574713.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21469..22326
                     /gene="nfo"
                     /locus_tag="ACHUNN_000221"
     CDS             21469..22326
                     /gene="nfo"
                     /locus_tag="ACHUNN_000221"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0574714.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22330..23418
                     /locus_tag="ACHUNN_000222"
     CDS             22330..23418
                     /locus_tag="ACHUNN_000222"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0574715.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23474..24724)
                     /locus_tag="ACHUNN_000223"
     CDS             complement(23474..24724)
                     /locus_tag="ACHUNN_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0574716.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24824..25765)
                     /gene="rihB"
                     /locus_tag="ACHUNN_000224"
     CDS             complement(24824..25765)
                     /gene="rihB"
                     /locus_tag="ACHUNN_000224"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0574717.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25895..26593
                     /locus_tag="ACHUNN_000225"
     CDS             25895..26593
                     /locus_tag="ACHUNN_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR0574718.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLLDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26660..27910)
                     /locus_tag="ACHUNN_000226"
     CDS             complement(26660..27910)
                     /locus_tag="ACHUNN_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0574719.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(28004..28942)
                     /gene="psuG"
                     /locus_tag="ACHUNN_000227"
     CDS             complement(28004..28942)
                     /gene="psuG"
                     /locus_tag="ACHUNN_000227"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0574720.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28930..29871)
                     /locus_tag="ACHUNN_000228"
     CDS             complement(28930..29871)
                     /locus_tag="ACHUNN_000228"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0574721.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30329..32020)
                     /gene="fruA"
                     /locus_tag="ACHUNN_000229"
     CDS             complement(30329..32020)
                     /gene="fruA"
                     /locus_tag="ACHUNN_000229"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0574722.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(32037..32975)
                     /gene="fruK"
                     /locus_tag="ACHUNN_000230"
     CDS             complement(32037..32975)
                     /gene="fruK"
                     /locus_tag="ACHUNN_000230"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0574723.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32975..34105)
                     /gene="fruB"
                     /locus_tag="ACHUNN_000231"
     CDS             complement(32975..34105)
                     /gene="fruB"
                     /locus_tag="ACHUNN_000231"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0574724.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34473..35654
                     /gene="setB"
                     /locus_tag="ACHUNN_000232"
     CDS             34473..35654
                     /gene="setB"
                     /locus_tag="ACHUNN_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0574725.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35651..35905)
                     /locus_tag="ACHUNN_000233"
     CDS             complement(35651..35905)
                     /locus_tag="ACHUNN_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0574726.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36060..36632
                     /gene="yeiP"
                     /locus_tag="ACHUNN_000234"
     CDS             36060..36632
                     /gene="yeiP"
                     /locus_tag="ACHUNN_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0574727.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36855..38321
                     /locus_tag="ACHUNN_000235"
     CDS             36855..38321
                     /locus_tag="ACHUNN_000235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0574728.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38439..39425
                     /gene="yeiR"
                     /locus_tag="ACHUNN_000236"
     CDS             38439..39425
                     /gene="yeiR"
                     /locus_tag="ACHUNN_000236"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0574729.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39464..40177
                     /gene="lpxT"
                     /locus_tag="ACHUNN_000237"
     CDS             39464..40177
                     /gene="lpxT"
                     /locus_tag="ACHUNN_000237"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0574730.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40589..41155
                     /gene="mepS"
                     /locus_tag="ACHUNN_000238"
     CDS             40589..41155
                     /gene="mepS"
                     /locus_tag="ACHUNN_000238"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0574731.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41336..42892
                     /locus_tag="ACHUNN_000239"
     CDS             41336..42892
                     /locus_tag="ACHUNN_000239"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0574732.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42974..44788
                     /locus_tag="ACHUNN_000240"
     CDS             42974..44788
                     /locus_tag="ACHUNN_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0574733.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44789..45883
                     /locus_tag="ACHUNN_000241"
     CDS             44789..45883
                     /locus_tag="ACHUNN_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0574734.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45883..46908
                     /locus_tag="ACHUNN_000242"
     CDS             45883..46908
                     /locus_tag="ACHUNN_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0574735.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46910..48499
                     /gene="yejF"
                     /locus_tag="ACHUNN_000243"
     CDS             46910..48499
                     /gene="yejF"
                     /locus_tag="ACHUNN_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0574736.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48503..48847)
                     /locus_tag="ACHUNN_000244"
     CDS             complement(48503..48847)
                     /locus_tag="ACHUNN_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0574737.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49180..50237)
                     /locus_tag="ACHUNN_000245"
                     /pseudo
     CDS             complement(49180..50237)
                     /locus_tag="ACHUNN_000245"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50328..51023)
                     /gene="rsuA"
                     /locus_tag="ACHUNN_000246"
     CDS             complement(50328..51023)
                     /gene="rsuA"
                     /locus_tag="ACHUNN_000246"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0574738.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51173..52933
                     /locus_tag="ACHUNN_000247"
     CDS             51173..52933
                     /locus_tag="ACHUNN_000247"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0574739.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53058..53342
                     /gene="rplY"
                     /locus_tag="ACHUNN_000248"
     CDS             53058..53342
                     /gene="rplY"
                     /locus_tag="ACHUNN_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0574740.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53481..54488)
                     /gene="yejK"
                     /locus_tag="ACHUNN_000249"
     CDS             complement(53481..54488)
                     /gene="yejK"
                     /locus_tag="ACHUNN_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0574741.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54670..54897
                     /locus_tag="ACHUNN_000250"
     CDS             54670..54897
                     /locus_tag="ACHUNN_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0574742.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54917..56677
                     /gene="yejM"
                     /locus_tag="ACHUNN_000251"
     CDS             54917..56677
                     /gene="yejM"
                     /locus_tag="ACHUNN_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0574743.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56752..56828
                     /locus_tag="ACHUNN_000252"
     tRNA            56752..56828
                     /locus_tag="ACHUNN_000252"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56786..56788,aa:Pro,seq:ggg)
     gene            complement(56931..58931)
                     /gene="yejO"
                     /locus_tag="ACHUNN_000253"
                     /pseudo
     CDS             complement(56931..58931)
                     /gene="yejO"
                     /locus_tag="ACHUNN_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14508 a        15377 c        14386 g        14660 t
ORIGIN      
        1 cttatccctt acggctaccg ggtcgggcgc gaggtaacat ccctgtaaaa cgcgccctca
       61 gcccacctcc atgtgggctg ccccggcctt cagggaacgc ctcggcaatt ttgacgccac
      121 caaacaaccg tgcggcctat tgataaagag ctaacacatt gtcaaaaaac atcactatgg
      181 ttttttagag tttctcgata tcaattgcct gaatagccct tgcaatatca ggggaattat
      241 tcaacacccg aacatgctga aataattccg ttgcttcatc gtattcttta cgcaaataac
      301 tcaaccactg tttaatccgc gcaacgtgat ataacccggt atcgccctgc ttttccagac
      361 gggtatattt ttgcagcaaa gcaaccacct ccggccacgg cattcgcggt tcgttatatt
      421 ttactacccg gctcaggttg ggaatattga gcgccccgcg accaatcatc actgcgtcgc
      481 agccgctgat cgcaatgcat tgttgcgcgc tctgccagtc ccagatttcg ccgttggcaa
      541 tcaccggaat attcagccgc tggcgaatct cgccaatcgc ctgccagtca atatgctccg
      601 cgcggtaacc ctgctctttt gtccgtccat gcaccaccag ctccgtagca ccagcctgtt
      661 gaaccgcatc ggcgatttca aatttcttct caccgctgtc ccagcccaga cgcactttca
      721 cgctgacggg caaatgcgcc ggtacagctt cacgcatcgc ttttgcaccc tggtagatga
      781 gttcaggatc tttgagtaac gtcgccccgc cgccgctgcc gttaaccgtt ttcgacgggc
      841 agccgcaatt gagatccacg ccccaggaac ctaattccac cgcacgggcg gcgttctctg
      901 ccagccattg tgggaactga cctaacaact gaacgcgcac cagcgtaccg gatggtgtcc
      961 ggctggcgtt ttgtagctca gggcaaatgc gatgaaagac ttttaccggc agcagttgat
     1021 ccaccacgcg gacaaactcg gtgatgcaca gatcgtagtc gttaacttcg gtcagcaatt
     1081 ctcgcaccag agagtcaagc acaccctcca tcggagccag taacacacgc atatcttcac
     1141 ccggaaaaaa atgaggcgct atgttagcgc ctatggtcaa cggattaaag gtctgcaaag
     1201 agggattgct gcaaactgta atccctctct atgcttgatg tatgcctgag ttcgttgcgc
     1261 attgcgtaat gccggggagc gaaaaaataa gcgctgattt cttaatgtga tcggtagcac
     1321 gttttacgaa ttaattgtat gatgaatcca tctcatctgg ggtgttgatt atgagtaaga
     1381 cactgaacat tatctggcaa tatttacgcg ctttcgtcct gatttatgcc tgcctgtatg
     1441 caggcatttt cattgcttcc ctgctaccgg taaccattcc gggcagcatc atcgggatgc
     1501 tgatcctgtt tgtcctgctg gccttgcaaa ttcttccggc aaaatgggtc aatccggggt
     1561 gctacgtact gattcgctat atggcgctat tgtttgtgcc gattggcgta ggcgtcatgc
     1621 aatattttga tttgctccgc gcacagtttg gcccggtagt ggtttcctgt gcagtcagta
     1681 cgctggtggt ttttctggtg gtgagctgga gttcacaact ggtacacggt gaacgtaaag
     1741 tcgtaggtca gaaaggatca gaagaatgat ggcgaatatc tggtggtcat taccgctgac
     1801 gttgattgtc ttttttgccg cccgcaaact ggcggcacgg tataagtttc ccctgcttaa
     1861 cccgttgctg gtagcaatgg tggtgatcat tcctttttta atgctgaccg gcatctctta
     1921 cgacagctac tttaaaggta gcgaagtgct aaacgatctg ctgcaaccgg cggtcgtcgc
     1981 gctggcctat cctttatatg agcagctaca ccagatccgc gcgcgctgga aatcgatcat
     2041 caccatctgt tttatcggca gcgtggttgc gatggtgacg gggacttccg tggcattgtt
     2101 gatgggcgct tcaccggaaa tcgccgcgtc aatcctgccg aaatcagtca ccacgcctat
     2161 tgcaatggcg gttggcggca gtattggcgg tattccggca atcagcgcag tttgcgtgat
     2221 tttcgtcggc atcctcggcg cggtatttgg ccatacattg cttaatgcga tgcgtattcg
     2281 taccaaagct gcgcgcggtc tggcaatggg gacagcctcg cacgccctcg gtacggcgcg
     2341 ctgcgccgag ctggattatc aggaaggtgc atttagttcg ctggcgctgg tgttatgcgg
     2401 gataattact tcgctgatcg caccgttcct tttcccgatt attctggcag taatgggcta
     2461 aaatttgcga tgcgtcgcgc atttttgatg taagtttcac gcgttgcata attaatgaga
     2521 ttcagatcac atataaagcc ataacgggtt cgtaaactgt tatcccatta catgattatg
     2581 aggcaacgcc atgcatccac gttttcaaac cgcttttgcc caacttgcgg ataacttgca
     2641 atctgcactg gaacctattc tggcagacaa gtacttcccc gctttgttga ccggggagca
     2701 agtctcatcg ctgaagagcg caacggggct ggacgaagac gcgctggcat tcgcactact
     2761 tccactggcg gcggcctgtg cgcgtacgcc attgtcgaat tttaatgttg gcgcaattgc
     2821 gcgcggtgtg agcggaacct ggtatttcgg tgccaatatg gaatttattg gtgcaacaat
     2881 gcagcaaacc gttcatgccg aacaaagcgc gatcagccac gcctggttga gtggtgaaaa
     2941 agcgcttgca gccatcaccg ttaactatac gccttgtggt cactgccgtc agtttatgaa
     3001 tgaactgaac agcggtctgg atctgcgtat tcatctgccg ggccgcgagg cacacgcgct
     3061 gcgtgactat ctgccggatg cctttgggcc gaaagatctg gagattaaaa cgctgctgat
     3121 ggacgaacag gatcacggct atgcgctgac gggtgatgcg ctttctcagg cagcgattgc
     3181 ggcggcaaac cgttcgcaca tgccttacag taagtcgcca agcggtgtcg cgctggaatg
     3241 taaagacggt cgtattttca gcggcagcta cgctgaaaac gccgcattca acccgactct
     3301 gccaccgttg cagggggcgt taattctgtt gaatctcaag ggttatgatt acccggatat
     3361 ccagcgcgcg gttctggcag aaaaagccga tgcgccgttg attcagtggg atgccacctc
     3421 cgcaacgctg aaagctctcg gctgtcacag tatcgaccgc gtgcttctcg cttaagcctg
     3481 gtgccggatg cggcgtgaac gccttgtccg gcttgccagc cctctcttgg tgtcgaaatt
     3541 cccggcaaac agtttgccgt ttcttgcgca aaaccagcgg gtaaagtagc ctgatggaaa
     3601 ttttccttag atcgagtctc ctgcatgtta aagcgcgtgt tcctcagcct gttagtcctg
     3661 atcggcttgc tgctgttgac tgtgctcggc ctcgatcgct ggatgagctg gaaaaccgcg
     3721 ccttatatct acgacgaatt gcaggatctc ccctaccgcc aggtcggcgt ggtgctcggc
     3781 acagcaaaat attatcgtac tggcgtaatt aatcagtatt atcgctaccg cattcaagga
     3841 gcgattaatg cctataacag cggtaaggta aattatctat tactgagcgg cgataacgca
     3901 ttgcaaagtt ataatgagcc gatgaccatg cgcaaagatt taatcgctgc tggtgtcgac
     3961 ccatcagata ttgttctcga ttacgcaggc tttcgtacgc tggattccat cgtgcgtaca
     4021 cgcaaagtgt tcgatactaa tgatttcatt attatcaccc aacgtttcca ctgtgagcgg
     4081 gcattattta ttgcgctgca tatggggatt caggctcagt gttatgccgt accgtcaccg
     4141 aaagatatgc tgtcagtacg tattcgcgaa tttgccgccc gtttcggtgc gctggctgac
     4201 ctttatattt ttagacgtga accgcgtttt ttagggccgc tggtccctat tccggctatg
     4261 caccaggtgc cggaagatgc gcaggggtat cccgccgtca cacccgaaca gttacttgaa
     4321 ttacaaaaga aacaaggaaa gtagttatgg atgtacagca gttttttgtc gttgccgttt
     4381 ttttccttat cccgatattt tgtttccgcg aagcatggaa aggctggcgc gcaggcgcga
     4441 ttgataaacg ggttaaaaat gcaccggaac cggtgtatgt ctggcgagca aaaaatcccg
     4501 gactcttttt cgcttatatg gtggcatata tcggcttcgg aattttatct atcggcatga
     4561 ttgtttatct tattttctat cgttaatatt ttctcttttc tgttaattat tgagaataat
     4621 tattacttca ctcaataagc tgcggatata acattctgaa ccgcagctat ttgtgaatcc
     4681 tttcacagtt taaatttccc cacacgctta gccttaatat cagcacgcaa tatttattta
     4741 gcgttcgcct taattactca tagcattaag gaagatcaca tgccgcaaca aaattatctg
     4801 gatgaactca ctccggcttt tacgccttta ctggcgatta aagaagcctc tcgctgttta
     4861 ttatgtcatg acgctccctg tagtcaggct tgcccggcac agaccgatcc ggggaaattt
     4921 attcgctcaa tctactttcg taattttaaa ggcgctgccg agacaattcg cgaaaataat
     4981 gccctcggtg ccgtttgtgc cagagtgtgc ccgacggaga aattatgcca aagcggttgt
     5041 acccgtgccg gtgttgatgc tcccattgat atcggccgct tacagcgttt tgttactgat
     5101 tttgaacaac aaaccgggat ggaaatttat cagcccggta ctaaaacgct cggcaaagtc
     5161 gcgattattg gcgcaggtcc tgccggattg caggccagtg tgacactgac aaaccagggt
     5221 tatgacgtca cgatctatga gaaagaagca caacccggtg gctgattgcg taacggcatt
     5281 ccgcaattcc ggttaccgca atcagtactg gatgcagaga tcgcccgtat tgaaaaaatg
     5341 ggcgtgacca ttaagtgcaa caacgaagtc ggtaacacac tcacccttga gcagctgaaa
     5401 gcagaaaacc gcgcggtact ggtcaccgtg gggttatcaa gcggttccgg gctaccgctg
     5461 tttgagcata gtgacgttga gattgccgtc gacttcttgc aacgtgcacg acaggcgcaa
     5521 ggcgatatca gcattccaca aagcgcatta attatcggcg gcggtgatgt cgcgatggac
     5581 gtagccagca cgctgaaagt tctcggctgt caggcggtaa cttgcgtagc gcgtgaagag
     5641 ttagatgagt ttccggcaag cgaaaaagag tttaccagcg cccgggagct gggcgtttcg
     5701 atcatcgatg gattcacgcc agtagccgtg gaaggcaata aagtcacgtt taagcatgta
     5761 cggttatcgg gcgaactgac gatggcggca gataaaatta ttctcgccgt cggtcagcat
     5821 gccagactag atgcctttgc ggagttagag ccgcagcgta acaccatcaa aacacaaaat
     5881 taccagaccc gcgacccgca agtctttgct gctggcgata ttgttgaggg agacaaaacc
     5941 gtggtctatg ccgtgaaaac cgggaaagaa gccgccgagg cgattcatca ctatttagag
     6001 ggagcttgct catgttaacg aaagatcttt cgattacttt ttgcggcgtg aagtttccca
     6061 acccgttctg cctctcttct tcgccggtag gcaactgcta tgagatgtgt gccaaagcct
     6121 acgacacagg ttggggcggt gtggtgttta aaacgatcgg cttttttatc gccaacgaag
     6181 tctcgccgcg ttttgatcat ctggtgaaag aagataccgg ttttatcggc ttcaaaaata
     6241 tggagcagat tgccgagcat ccgctggagg agaatctggc cgccctgcgt cggctgaaag
     6301 aagattaccc ggacaaagta ttgatcgctt cgatcatggg ggaaaatgag cagcaatggg
     6361 aggagctggc gcgcctggtg caagaagctg gcgcggatat gatcgagtgt aacttctcct
     6421 gtccgcaaat gacttctcat gcgatgggta gcgatgtcgg gcaaagcccg gagctggtag
     6481 aaaaatattg tcgggcagtg aaacggggtt ccacgctgcc aatgctggcg aagatgacgc
     6541 cgaatatcgg tgatatgtgc gaagtggcgc tggcggcgaa gcgcggcggc gcagatggca
     6601 ttgcggcgat taacaccgtt aaatccatca ccaatatcga tcttaatcag aaaatcggta
     6661 tgccgatcgt taacggaaaa tcaagtattt ccggatattc cggtaaagcg gtaaaaccga
     6721 tcgccctgcg cttcattcag caaatgcgca cccatccaga actgcgcgat ttcccaatca
     6781 gcggtatcgg cggcattgaa acctgggagg atgcggcgga gtttttattg ctcggcgcag
     6841 caacgttaca ggtcaccacc ggcattatgc agtacggcta tcgcatcgta gaagatatgg
     6901 cgagcgggtt atcgcattat ctcgccgatc agggatttga ttcgttgcag gagatggtag
     6961 gtctggcgaa taacaatatt gtcccggcgg aagatttaga ccgcagttat attgtctatc
     7021 cccgtatcaa tcttgacaaa tgtgttggct gtggacgctg ttatatttcc tgttacgacg
     7081 gcggtcacca ggcgatggaa tggagcgaga aaacccgcac accgcattgt aataccgaga
     7141 aatgtgtggg ttgtctgctt tgtggtcacg tctgcccggt gggttgtatt gagctcgggg
     7201 aagtgaagtt taagaaaggc gagaaagaac acccggtaac gttgtagaac ccgcgtcaca
     7261 tccggcattt ggttcctgtt gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc
     7321 acacagatca tgatgttatg tgcgtttatg accgaatgcg gaccacattc acatcatttc
     7381 ttacgcgcgt atttcagtga atccagcgct acggcgaaga taataatcgc ccctttgatg
     7441 atgtactgcc agtatgggtt tacgccgata tacgtcaggc catagttgat gacggtaaaa
     7501 ataatcaccc cggtcaccac gccaatcacc gtccccacac cgccgctgaa cgatacaccg
     7561 cctaccacgc acgccgcgat agcatccagc tcatacataa agccgaggtt gttggtggca
     7621 gagccgatac gtccggcttc taacatcccg ccaaaggcat agaacacgcc agacaacgcg
     7681 tagatcatca gcaggttcag gccgacgttg acaccagata cttttgccgc ttccgggtta
     7741 ccgccaatgg caaaaatgtt cttaccgaag cgggttttgt tccacaacac ccagacgaac
     7801 gccaccgcaa tcaacgcgta gaaggtgatg taagagagac ggaaactccc cagcgcgaca
     7861 aagccctgag caaaggtaga gaagccactg tcaaaaccag aaattggcga cgccccgaca
     7921 aagtcgtaat agagcgagtt gatgccatag acgatgatca tcgtgccgag cgtggtaatg
     7981 aacggcgtca cgttgagata agcgataatc agaccgttga tcaaaccgat caccgcacca
     8041 atggcacaga caatcagaat aaccagcgca atcggcatcg tcgccatttc cgggaacact
     8101 ttgttggcgt tatccatgga ctgcaataat gtcgcagcca ccactgccgc cagccctacc
     8161 tgacgaccag cagaaagatc ggtcccctgg gtgacaatta accctgccac accgagcgcg
     8221 ataataatac gcaccgatga ctgggtgaga atattactta agttcaacag acttaaaaat
     8281 gttgggtcct ggaaaataat aatcgccagc aaaactaaaa gaacgacgta aataccgccc
     8341 tctttcaggt aagtaagaaa acttttctta tttaacgcac tcatgggaag cccctaatct
     8401 taaaggtgca aagacgcaag acgcagaatt tcgttttgcg ttgttgtttt tgtatcgaca
     8461 attccggaaa cgagaccatt gctcatgacc agaatacggt ctgttatccc taacaactca
     8521 ggcatttcag aggagataat aataatcccc ttgcctttct tcgccagttc ggcaattaac
     8581 tgataaattt caaacttcgc cccgacatca ataccgcgcg tcggttcatc gagcattaat
     8641 atttctggtt gcgttagtag ccagcgacca ataatcacct tttgctgatt accaccggag
     8701 agcgaaccaa tttgcgtccg atgacccggc gtttttaccc gcatcgaatc aatcacccac
     8761 tgggtatcgc ttttcatccg cgagttatcc agtaaaccaa ctttattttt gtagttgcga
     8821 atattggaaa ttaacgagtt aaaaccaata tccagatagg cataaattcc cgttgagcgg
     8881 cgctcctcag ttaccagtgc aaatccgtgg tttatggctt cgttggcatt atggttattg
     8941 atctgtttgc cgtgcaacgt aatggtgcca gccgatttct cgcgaatacc aaataacgtc
     9001 tcaacaatat cggtacgttt cgcccccacc agcccggcaa taccgaggat ctccccttta
     9061 tgcagatcaa acgagacatc gcgaatcgac ggctggcgca gtgacgttag gttacgcacc
     9121 tcgaggatga cttcgcccgg cttgttttct ttgtcaggaa aacgctgatt aagagaacgc
     9181 ccaaccatca tggcgatgat cttgtccatc gtcagtcctg ccagcggttc ggtggcgatc
     9241 cactgaccgt cgcgcaatac ggtaacttca tcacataact ggaagatttc ttccatttta
     9301 tgcgagatat aaacaatacc gcagccgcgc tcttttaatt tacgaataat agtgaacaga
     9361 tgattgacct ctttttcggt taacgaagaa gtcggttcat ccataatcac aattttcgcg
     9421 ttataggaaa acgctttggc gatttcgatc atctgcattt gcgaaacgga taatgtgccg
     9481 acgcgcgcac gcggatcgat atcaatatcc agttcatcaa aaattgcttt ggtttcgcgg
     9541 tacattttgt cctgatcgac aaacatgcct ttggtgggat atcgccccag ccacatgtta
     9601 tccatcaccg aacgttgtaa taccaggttt aactcctggt gtaccatcga aataccattt
     9661 tccagtgcct ctttggcgga atggaaatcg atctctttac cctggaataa aatggtgccg
     9721 gagtcttttt gataaatacc aaacaggcat tttaataatg tcgattttcc tgcaccgttt
     9781 tcccccatta atgcatggat agaatgtggc cggactttta aattaacgtt atcaagtgcc
     9841 ttaacaccag gaaaggactt gttgataccg ctcatttcca acaagtattc cccggaagac
     9901 ggagtcgttg agctgaccat ataattttac cttgttggcc atacaataag ggcgcagtaa
     9961 acgactgcgc ccaatcagtc ttatttcttg ctgaattcag ccaggttgtc tttatctacg
    10021 ccaacataag gtacgcggac cactttgttg tcgattttcc agttggtgcc atcagccgca
    10081 cctttgccat cggccaggtt tttcgccaga tcaaaggtcg ctttcgcctg gttgttagca
    10141 tcgttcagta ctgtgcccgc cagtgcaccg gatttcacca gcgccagcgc ttctggcagc
    10201 gcatcgacgc caaacaccgg aatgctggac ttgttgtgtg ctttcaatgc ttctaccgcg
    10261 cccattgcca tcgcatcgtt gttggcgata accacttcga ttttgttggc gttcgggcca
    10321 gacagccagg cgtccatctt atctttcgcc tgagcggtgt cccacattgc ggtatctaac
    10381 tgtaactgtt cagttttgat gcctttatcg ttcaattctt taatcacgta agtggtacgt
    10441 gcttctgcat ccggatggcc cggttcacct ttcagcagta cgaactgaat ctgaccgtct
    10501 ttgttcagat cccaaccctg attcgccgcc cagtgtttag caatcaaatc gccctgaata
    10561 atgccggact ctttggagtc agtgccaacg tagtaggctt tgtcgtagct atccagcgcc
    10621 ttacgagacg gttctttgtt gaagaaaacc accggcacgt tttgcccacg cgctttctca
    10681 atcaccgtac ccgcagctgc cgggtcaacc aggttgattg ccagtgcctt cacccctttc
    10741 gccagcaata cgtcgatctg atcgttctgc ttggactggt cattctgaga atcattcatc
    10801 agcagctgaa catctggcgc ggctttcgca tcttgctcaa tagccttgcg cactacagac
    10861 ataaagttat cgtcgtactt atagattgtt acaccaatgc gagtatcagc agcgtgtgca
    10921 gcggcaccga ataacatgct ggccatcaca gcagacaggg ttaacacctt cttattcatg
    10981 gtatctccgg tttttcttat gcagggtagt gcttgagata aatgctcggc ggggcagtag
    11041 agttaatgaa gtgttactga acgccgaagc tcactttttt aaattcgttc ttccatgccc
    11101 ggtaacgctc cagaaaacgg ctttaattgt tgttatgacg ctattacctc ggtgaaagtg
    11161 attattcact gttacatacg ggttacaacg ttaaaacggt gcaatcatag ctatcacatt
    11221 gttaagatac tgtgaaatca ctcacagatt gaaagcggtt acatcgcctg atttgttgag
    11281 ttagtgatcg ccgccgcatt ctggcgcgtt gcgacagaat ggcgacgcac taacgtcggc
    11341 ataaaacagt ggctggcacg aggatcaata ttgcctgctg ccccctgcaa ggccagttcg
    11401 gtggctaatt tagccattga agcaatggga taacgcacgg tcgttaattg cgggtcggtg
    11461 taacgggcga tgggaatatc atcgaaaccg atgattgaga gatgtaacgg aatcgcaatg
    11521 ccattatctt ttaatgctgt cagtgcgcca gcggccatat tgtcgttata agcaaatata
    11581 gcggtaagtt gtagattgcg ccccagcagt tcaaccatcg ccgcctcacc gcccggcatg
    11641 tccggcgtac cagtgccaat ccagctttcc ggcggaataa tatcctgctc tttcaacgca
    11701 ctcatccagc ctgctttacg catggcgtca tcttcaatgc cgtgactgga agaaagataa
    11761 ccaatacgtt gatgaccgtt attcagcaac atgcgcgtcg ccattcgggc accgctgaga
    11821 ttatccaggc aaacgcaacg atgggcgtac cccggcacaa cgcggttgat taacaccata
    11881 ccgggaatgt tatccataaa ttgcgccagt tcatcgtcac tcaatgcttt tgagtgaaca
    11941 atcaacgcat tacaacgctg gcgaattaac acctcaatgg cgtgacgctc tttttccgct
    12001 tcatgatagc tattgccgat tagcacgtat ttctgatgct gctgagcgac tagatccacc
    12061 gcttttacca gcgcgccgaa aaacgcatca gaaacgtcca tcaccaccac gccaatggtg
    12121 tcgctaacct gagttgccag cgcctgggca ttggcgtttg gccgataatc cagctcactc
    12181 acggctttca ttactgcttc acgcgtgtcg gcactgacca gcgtgctgtt attgagcacc
    12241 cgggaaaccg ttgccacaga gacgccagcc taacgcgcta catcacgaat ggtgatcata
    12301 ttcactacct gttatgagat tgcagtaaat gactgcttgc tggcggctat tttgtcagca
    12361 ctgaatacag gacttcgtga atcgagtcac agcaatggaa acggttacag ccgttttgtt
    12421 aacgaatgtg atccagatcg ttatcttgat gttttagata ttgccgttcc ggcagcacgc
    12481 aaagttaact gacgccagag ccattccacc ggcccctggc ggaagtaacg cagccagata
    12541 acagagaaga ggatattcgc cagccatacc ggaataacaa acgccagcag ctccaggcgg
    12601 tcaaaatgca taaacaaacc gaggtggtaa aaaagcatgg tacaaatcag cgtttgcaat
    12661 agatagttgg tcagcgccat ccgtccgacg caggcgatcg caagcaccag cttaaagcgg
    12721 ctcaattgcg gccagaagcc ataaaacagc gacgcatagc caatcgcctg aaacggcgca
    12781 ctcagttccc gcggcatttg aagtaagaag gcacaccagc gatacgccca gtccagctgc
    12841 cattgcaggg cgatggcagg aaggttaatg gtcaccccaa tcgccaccag cacaaaacca
    12901 gtacgacgat agtgacgtaa gctgaactgc cctttcagcc agccgctgcg catcagcgcc
    12961 gcaccaatga gcatcatccc tgccagttgc cagccatact gtgcgcccag tgccagtaaa
    13021 ctgttgccaa caccatcggc acgattactg atcgcttcaa cgccgccgtg aagcttccag
    13081 tatttttcat acaaaatagc cgatgcatcc ggcgtccagg tgcggctggt ctggctgtcg
    13141 gaaatcagcc ccaataacag caaaacgcca agccccacca gataaagcat gacgcctgta
    13201 ttaaacaggc tttttaccga tggcgcatcg cgcaccagcc gccagcagat taagcccacc
    13261 agcccgtaag ccagcagaat atcgccgtcc cagaacaata aaccgtgaat aaaacccagc
    13321 aagactaaca gcgttaaccg cgactggatc cagcgtctgc cacggggcag caacatttgc
    13381 aggcccgcac caaacagcag cgcaaaaagc gtgaggaatt tcacctggcc gatgagatcg
    13441 agaaatgccc aggtccatgc atcctgcggc gtaatagcgc cgtaccaggc gggattgaga
    13501 taagccgcct ttggtagccc aaaggcgctg atgtttaata gcaggatccc cagaatggcg
    13561 acgccgcgaa caaaatcgag cgttacgttg cgctccatgg ttcctgcctt ttaatcagtt
    13621 gtgatgacgc acagcgcgca gaaactcgtg gcgcgtattc tgactggatt tgaacaatcc
    13681 accaagagag gtcgttgttg tggcactggt tgcatcgcgg atgccacgcg ctttcacgca
    13741 gtaatgcacc gcatcaatgg agacagccac gttattggtg cccagcagcg tttgtagcgc
    13801 aataagaatt tgctgcgtca gacgttcctg cacctgcgga cgctgggcaa agaactgcac
    13861 gatacggtta atttttgaca gaccgatcac cgaatctttc gggatataag ccaccgtcgc
    13921 tttgccatcg atggtaacaa aatggtgttc acaggtactg gtcagagtga tatcgcgcac
    13981 ggtgaccatt tcatcgacct tcattttgtt ttcaatgagg gtgattttcg ggaagttggc
    14041 gtaatccaga ccggagaaaa tttcatcgac atacatttta gcgatgcgat gcggcgtttc
    14101 catcaaactg tcatcagcca ggtcgagatt cagcagctgc atgatttcgg tcatatgacc
    14161 agcaataagg cttttgcgcg tttcgttatc catttcatgc acgggcgggc gcagcggtgt
    14221 ttccagtcct cgcgcaacta acgcttcatg aaccagggcc gcttctttac tgagtgatgg
    14281 catttatgat ttctcctgca ggtgtgacgc ctccgccctg cgtgggggca aagttattaa
    14341 gctgatttac agcctgatta ttgtgcgtga ggcggcgcac ataatccagt attcacagcg
    14401 ataattattg taattgccgc tgactttcat cagcagatgt taaaacatcg ttatgcaaat
    14461 acggaagtga aagttactca cagcacattg aataaacggt atgatgaaga aattgcaaac
    14521 aacacaacaa ggagccacgc atggaaatgc tcgaagagca ccgctgtttt gaaggctggc
    14581 agcaacgctg gcgacacgac tccagtacct taaactgccc gatgacgttc agtatctttc
    14641 tcccaccccc tcgtgatcac accccgccac cagtgctgta ctggctttcc ggattaacct
    14701 gcaatgacga gaacttcacc accaaggcgg gtgcccagcg cgtggcggca gaactgggga
    14761 ttgtactggt gatgccagac accagcccgc gcggcgaaaa ggttgccaac gacgatggct
    14821 acgatttagg ccagggcgca ggcttttatc ttaatgccac gcaaccgccg tgggcgacgc
    14881 attaccggat gtatgattat ctgcgcgatg aattaccggc gctggttcag tcgcaattta
    14941 atgtcagcga ccgctgcgcc attagcggtc actcaatggg tggtcacggt gcgctgatta
    15001 tggcgctgaa aaatccgggt aaatacacca gcgtttcggc ctttgcgcca attgtgaatc
    15061 cgtgcagcgt cccgtgggga atcaaagcgt ttagcaccta tttaggtgag gacaaaaatg
    15121 catggctgga atgggatagt tgcgcgctga tgtatgccag taacgcgcag gatgcgatcc
    15181 cgacgcttat cgatcagggc gataatgatc agtttcttgc cgaccagttg caacctgcgg
    15241 tattggcaga agccgcgcgc cagaaagcgt ggccgatgac gctgcgtatt cagccgggat
    15301 atgatcacag ttactacttc atcgcctctt ttatagagga tcacctgcgc ttccatgcgc
    15361 agtatttact gaagtgaaag tccgcccggt tcgccgggca tcttctcatc agaagcgata
    15421 atccactgcc ataaagtaac gacgtccgtc ttcgttatag ctgtagtcgt cacgactgag
    15481 atccttgtcg ccaaggttca gcacgcctgc gcgcagtttg acgtctttag tcacctgcca
    15541 ggccgcgccg gtattccaga tggtataacc gcccggtgtt ttagccgtcg cgctgtcggc
    15601 gcgtttctgc ccggtatagt gaccagaaac atagaatgac cagtcttcca gcgccagcgg
    15661 tttccagtcc agcgtaccgt tagcagtatg gaacggcaga tcggatagcg gtttgttttc
    15721 gccgttgctg acatcacgac catcgttgta ggtgtagttg atcgacagtt tccattcatc
    15781 gttgaacgga attttcagtt cggtttccac gccctgaata cgagctttgt taacgttgta
    15841 gtagctaaat accggtatgc gccgtccgtt agcgcccgtc tcaaaaccaa caaagttttg
    15901 gtagcccggt gcagcgttga cgtcagacgt acggctgatg ctgatacgat ctttcacatc
    15961 gttacggaaa acggtaacgc tggattcaac cccttccagc cagccttctt cacccatgta
    16021 gtaaagcccc agctcccaac tttcgctggt ttctggtttc agatccgggc tacccacaat
    16081 cttacatgcg ccacggcagg aattgctcgt ccagtcaggg ctaagttgca acagagaagg
    16141 tgctttaaat gccgtcgccc agcccccttt caccgttacg gtgtcggtgg cgttataaac
    16201 caggtaggca cgcggactcc agtgttcacc gtaggtttcg tgatcgtcca tacgcacgcc
    16261 ggtcgtcagc gccagcggct cgaagatccg ccattcatct tccacaaaca gcgcgtactg
    16321 gctggcagac gttttggagc tggttccccc ggtcaggttc accgcatcgc taagtttgtc
    16381 gtgacgccat tcaccgccaa ccgtgagaaa ctgattaatc gccgtcagcg gcaacgtgta
    16441 tttgccgtcg accgtattgc tttcggaagt tatcgggctg ctgttgccag ggtttttgtt
    16501 ctcgactttc tcaccgtagt atttcagttc gctggtgccg taatcccaac gcccattatg
    16561 gctgacggag tagttctggc gttccaggcg gtttttgtcc agcgagtcgg aatcacgatc
    16621 ctgacggtcg aaaccgtatc cggcagtaaa atcgtgattt tgattcggtg tccaggcaaa
    16681 ttcgacattg ccgtcgcggc tggagaatcc ttcaatacgc ggcgtttctc cggtatcggt
    16741 ggtcgttgag ttttgcgggt catccttttc acgttttgcc aggctgccgt aagctttcat
    16801 tcccagcaca ccatcaatta atggtccact ggtaaagaac tgaccgttat aggtatcacc
    16861 gcgatcgcga tgttcctgaa tggtggtatc gacggtaacg gtacccgacc atttctgacc
    16921 gatttttttg gtgatgatat tcactacacc gccgagcgca tcggaaccgt acagcgacga
    16981 catcgggcca cggaccactt caatacgttc gatggaatcg accgggatcc agttcagatc
    17041 gaaatcattg tggcggaaga cggcattgcg ggagttcacg cgtttaccgt cgacgagaat
    17101 cagggtatag ctgctgtcca gaccacgaat actaacgccc ttacggttat ccccttcgtt
    17161 cgtcagttgt acgccaggca cttctttgag gacatccttc agattctgta ccggttttcg
    17221 ctgcagatct tcctgggtaa tgacgctgat actggcgggt gcatctttaa ggttttgttc
    17281 aacggaagat gcagtgacaa ccatcgtttc gccatcgtca tcgaccgcta acacaggcca
    17341 tacacaagaa atagcggaca aacacagccc gacccgtacg aaagggttca acctaaacat
    17401 tccatatctc catgaggtaa ctacgaaaat aaaatgggta tcgctcacat cttctttacg
    17461 tccccttgcg tacggcagca tcgcggtgga cttatttgta tgcaggtgat catcccagaa
    17521 agccggacca gcctctgatt tgtcagcctt ttttgattgt agcgtaacga taatgcaaac
    17581 gataacaatt atcaatccaa atgttaaatt ttatatccgc atgatttgtc agggaaatgg
    17641 aattgaaaaa gccctctcgg ttgagagggc ttagcaagga agggaggaaa cttatttctt
    17701 atcgttctgc gggaacttca tttcgctgta gcgtacgaag tgagttcctt taatcagctt
    17761 gtagccgaac caaataatca ggaacagcgg gataccaata tacgttgccg ctacgccgcc
    17821 ccagtcaatg gtatctttca ggaacgcttc gtagttctgg cccaaagtga taatcagaca
    17881 cagaatgaat gcgaagatcg gccccagtgg gaagaaacct gaacggtacg gcagatcgtt
    17941 aatgtcgtgt ccctgcaata cgtaaccgcg acggaagcga tagtggctaa tggcaatccc
    18001 cagccaggcg ataaaacccg tcatcccgga ggtgttcagc agccacaggt ataccgtctg
    18061 gttgccaaac atggaggtca ggaagcacaa accggcaatc accgtcgtcg catacagcgc
    18121 attacgcggc acgccaccac gcgacaattt agcgaaaatg cgcggcgctt taccgtcaca
    18181 cgccagggtg tacagcatac gagtagacgc atacataccg gagttacctg ctgacagcac
    18241 cgccgtcaga ataactgcgt tcatcaccgc cgccgcagag agcagacccg cgtgctggaa
    18301 caccaaggtg aacggactga cgctgatgtc tttaacatcg ttacgcagca ggctcggatc
    18361 ggtatacgga ataatcaggc tgataatcag gatcgcgaac acatagaaca acaggattcg
    18421 ccagaatacc tgacgtaccg cgcgtggaat gtttttcgcc ggatcttcgg actcgcctgc
    18481 agcaataccg atcagctcgg ttccctggaa agagaagccg acaatcatag ctacgccgat
    18541 catcgccgca aaaccaccag caaacggcgc ttcgccgatt gtccagttgc tccagcccgc
    18601 aggctgcgcg cctttgaaga taccgataat catcagcacg ccaacgatga taaagacaat
    18661 aactgtcgtg actttgatca gtgaaaacca gtattccgct tcaccaaagc cacgaactga
    18721 gatgtagttc agcaggaaga taacgccgag gaacaacgca ctccagatcc agcccggtgt
    18781 atccgggaac caccagctca tgaccagctg tgctgcaacc aagtcaacgg caatagtcac
    18841 cgcccagttg taccagtagt tccagcccag cgcgaagcca aagccttctt caacatagtt
    18901 ctgaccgtaa gtggcaaacg aaccggaaac cggcatatat gcagccaatt caccgagact
    18961 ggtcatcagg aagtaaacca tcaggccaat cagcatatac gagagcaatg ccccgcccgg
    19021 acctgcctga gaaatcgttg cgccagaggc aacaaaaaga cctgtaccga tggaaccgcc
    19081 aatggcaatc atcgtcaggt gacgcgcctt taattcacgg cgtaagcccg gcgcttctgt
    19141 agttttagtt tcggaaccca tacaaaaatg ctatccatct taaaaatgag gcgcgattgt
    19201 agcagacgat tggcaatcct tccggcagaa atacacggtt ataagacacc ttcatgatcg
    19261 cccagggatt ataagtaaag cagccaatct cttttctggc gaatgcatgc tgagggcgct
    19321 gcgtcacgaa atcgacacag caccagcatg ttcttgtaca gcaccttaac gcggtacatt
    19381 cgcgggatcg caatagtcca gaaagcgccg tagcgcgttg gaaaggtgtt tttgccgatg
    19441 atgtatacgc cacaacgtac gcatcaggcg cgggagaggg accgcaactt cacttaatgt
    19501 gcctgcctgc aattgatctt caatcacacg tcgcgacagg caactaattc ccaacccatg
    19561 acgcaccgca tgtttgattg cctcggagtt acctaattcc atcgccatct caaacttcgg
    19621 taaatgtgac agcaacagat aatcgacaat ctcccgcgtg ccggaaccgc gttcacgcag
    19681 gatccacggc gcagcggcca gctgttctaa ggtgaccgga ccacgagcca acggcgaagt
    19741 cggcgcggcg aaaaccacca gctcgtcttc cagccacggt tcagaaatga tttcagtgct
    19801 gtggcacggt ccttcaataa agccaatatc aacgcggaaa tccagcaccg cttgcatcac
    19861 gtcctggctg ttcccaacgc taagttcaat cggcaactgc ggataatcat ggcgataacg
    19921 ggcgataact gcaggcagaa tgtagttacc gatggtacta ctggcataga tacgaatcgc
    19981 gccgttgtct tcgcgaaaca gttgttcgat ttcaaccgcc tgttcaagca atgccaacgc
    20041 acgcggatag agcagccgcc cgtgttcatt aacaaccagt cttttcccca cgcgatcaaa
    20101 cagttgcacg ccaagctgcc cttccaggtc ggtcaaggct gcgctcactg ctgattgcga
    20161 caacgccagc atcaccgacg cctgggtggt tgatccactt ttcaatactt ctgcaaaaac
    20221 ttccaactgc cggagggtga tgtgcatagt cgcttaccac ttataaagat taattataaa
    20281 tatataatca attttatttt taaaccagtt agtcgtaacc ttatgcccgg ttaaaggaga
    20341 gggttatgac gaatatcacc ttacagaaac aacatcgtac actgtggcat tttattccgg
    20401 ggttagccct gagtgcagtt atcaccgggg tcgccctgtg gggtggttcc attcccgcgg
    20461 ttgcgggtgc cgggtttagt gccctcaccc tcgcaatctt gttggggatg gttttaggca
    20521 acaccatcta tccgcacatc tggaaaagct gtgacggtgg tgtgctgttt gccaagcaat
    20581 atttattacg tctgggtatc attctttatg gcttccgtct gacgttctcg caaattgccg
    20641 atgtcggtat cagtgggatc atcattgacg tgttgacgct gtccagtacc ttcctgctgg
    20701 cttgcttcct ggggcagaaa gtgtttggtc tggataagca caccagctgg ttgatcggtg
    20761 ccggtagcag tatctgtggt gctgccgcgg tactggcgac tgagtcggta gtgaaagcgg
    20821 aagccagtaa agtaaccgtg gctgttgcaa ccgttgttat cttcgggacc gtcgcgattt
    20881 tcctctaccc ggcgatatat ccgctgatgt ctcaatggtt tagtccggaa actttcggta
    20941 tctacattgg ttctactgtg cacgaagtgg cgcaggtggt ggcggcaggt catgccatca
    21001 gcccggatgc ggaaaacgca gcagttattt ccaaaatgct gcgcgtgatg atgctggctc
    21061 cgttcctcat cctgctggcg gcgcgtgtta aacagctgtc tggggcgaac agcggcgaga
    21121 aaagcaaaat cactattccg tggtttgcca tcttgttcat cgtcgttgcc atctttaact
    21181 cgttccacct gttaccgcag agcgtggtga acatgctggt aacgctggat accttcctgc
    21241 tggcaatggc gatggcggcg ctgggtctga ccactcacgt cagcgcgctg aaaaaagctg
    21301 gggcgaaacc tctgctgatg gcactggtac tgtttgcctg gctgattgtt ggtggtggtg
    21361 cgattaacta tgtgattcaa agcgtcatcg cataaaccac tacatcttgc tcctgttaac
    21421 ccgctatcat taccgttttc ctccagcggg tttaacagga gtcctcgcat gaaatacatt
    21481 ggagcgcacg ttagtgctgc tggcggtctg gcaaatgccg caattcgcgc cgtcgaaatc
    21541 gacgcaaccg cgtttgcctt gttcaccaaa aaccaacgtc agtggcgtgc cgcaccgctc
    21601 acgacgcaaa ccatcgatga attcaaagcc gcctgtgaaa aatatcacta cacatcggcg
    21661 caaattcttc cccacgacag ttatctgatt aacctcggac atccggtcac tgaagctctg
    21721 gaaaaatcgc gcgatgcctt tatagatgaa atgcagcgtt gcgaacaact ggggctttct
    21781 ttgctcaact tccaccctgg cagccatctg atgcagattt cagaagagga ttgccttgcg
    21841 cgtattgccg aatccatcaa cattgcgctg gataaaactc aaggtgtaac agcggtgatt
    21901 gaaaacaccg ccggtcaggg cagtaactta gggtttaaat tcgaacatct cgcggcgatt
    21961 atcgacggcg tggaagataa attccgcgtc ggcgtctgca ttgatacctg ccatgctttc
    22021 gctgccgggt atgatttgcg tactccagcc gaatgcgaaa aaacgttcgc tgattttgcc
    22081 cgtattgtcg gctttaagta tctgcgcggg atgcacctta acgatgcgaa aagtaccttt
    22141 ggcagccgcg ttgaccgcca tcatagcctc ggtgaaggca atatcggtca tgatgcgttc
    22201 cgctggatca tgcaagacga ccgtttcgac ggcattccgc tgatccttga aaccatcaac
    22261 ccggatatct gggcagaaga gatcgcctgg ctgaaagcgc aacaaactga aaaagcggtg
    22321 gcctgatcga tgaacgacag ggaaaagcag attcttaaaa ttttacggcg taacccgctg
    22381 attcaacaga atgaaatcgc cgatattctg caaatcagcc gctcacgtgt tgccgctcat
    22441 attatggatt tgatgcgcaa gggtttgatt aaaggcaaag gatatatcct taccgagcag
    22501 gattactgtg tggttgtggg tgcaatcaat atggatattc gcggaatggc ggatatccgt
    22561 tacccgcaag cagcctctca tccaggttct gttcactgtt ccgcaggcgg cgtcggacgt
    22621 aatatcgcgc ataacctggc tctgcttggt cgggatgtgc atttaatatc agctattggc
    22681 aacgattttt acggcgagac attactggaa gaaacgcgcc gcgcgggcgt gaacgtttca
    22741 aactgcattc gtctgcatgg acacagcact gcaacttatc tcgctatcgc taataagcag
    22801 gaagaaacta tcctggcaat caatgacaca catattctgc aacagctaac gcctcaactg
    22861 ctcaatactt cacgggactt gattcgccat gctggtgtgg tgctggctga ttgtaatctc
    22921 acacctgaag ccctggagtg ggtctttacc attgctgatg aaattccgat gtttgtcgat
    22981 accgtttccg aatttaaagc gaacaaagta aaaaactggt acagccggat ccacactctg
    23041 aaaccaacac aaaatgaact ggaaattttg tgggggcaac cgattaaaga cgacaacgat
    23101 cgcatcagag ctgtaaattc gctgcatcaa caaggtgtta aacgcatttt tgtttatctg
    23161 aaagatgaat ctgtattttg cagcgataag gatggagaac aatttttgct gaccgcgcca
    23221 gcgcatacga cagtagacag ttttggtgct gacgatggtt ttatggcggg cctggtatat
    23281 agctttcttg aaggatacag tttccgcgac agcgcccgtt ttgcggtagc ctgcgcggca
    23341 atttcgcgcg ccagcggcag cttaaacaac cctaccctgt ctgccgataa cgcgctttca
    23401 ttagtgccaa tggtgtaaca atgttgccgg attcgacgct tgaccgcatc ttatccggct
    23461 cataaattgc gcactacgct aaaccaataa agaatcctgc aatagtagca ctcatcaggt
    23521 tagaaagtgt cgccgccgcc agcgcgcgta aaccaagttg ggcgatttcc ggcgcacggt
    23581 gtggcgcaac cgcagaaaac gcccccacca ccaccccgat agaaccaaag ttagcgaaac
    23641 cgcacaacgc gaaagaaata atcgccacgg ttttagcatc cagagtgcca cccgtttgca
    23701 gatatggcga gaaattgaga taagcgacaa attcattgat cgccagcttc tgcccaatca
    23761 aactcccggc aagatttgca tcactccagt caacccccat cacccacgcc aatggggcca
    23821 acaggtaacc taaaatcgac tccagcgaag catgttcaaa accaaaccag ccgccaacac
    23881 cgccgataat accgttaatc aacgcaatta ttgcaacaaa tgccataaca actgtcgcta
    23941 caccggcggc gattttcagc ccggtcattg cccctgtcgc agcggcttca ataatgcttt
    24001 ttggcggtgt ttcggtgaaa gagaggttat taaaagaaac ctgcgaagat tccgtagccg
    24061 ggcttaacag gcgggcaaac aagatcccgc cagggatcgc cattaacgat gccgccagca
    24121 aatattccac aggtacgccc agtgcggcgt aaccaatcat tgtcgaacca gcaatcgagg
    24181 ccatgccact acaaatcgct gtaaataatt cattgcgatt cagacgatcg ataaagggtt
    24241 tgacgatcgc cggaatttcg ttttgcccga ggaaaatggt ggtgaccgcg acgaatgact
    24301 cgatcttgct gatatttaaa gctttctgga agataccgcc gagaatgcga attaagatcc
    24361 ccatcacacc gatgtagtag agaatactca ccagtgcagt gacgaagata attgccggta
    24421 ataccctgaa accaaagata aatcctgcgc catcaaataa ggtatccatt ttcggtccga
    24481 ccagagaacc gaagataaat gcgctacctg cgtcgctgta cgccatcact ttgtggacgc
    24541 caaaagcgac tttttctgcg acccaacgcc ctggcggtaa ccaaagcata atcccgccaa
    24601 tcacgacctg taacactaac gccgcgccaa cggtacgcag gctgatcttc ttcttgttta
    24661 ctgacagtaa aaacgcaatt gccagcagta ccaccattcc cagaacactt ctcatgacat
    24721 ccatagtgat tatctcttca tgtcattttg ggcgacggct ttctgcccat cagaaatagc
    24781 cgggcctgca tcttgcgata ctgtcccggc ttgttatcaa cacttaatgg gtttttatgt
    24841 agccgcgtac gcactcttcg actaatcccc agaaccagtc ggtatcaata gtgatgccga
    24901 ctttagtatt ggcgggcttg ccaagaacgc ccagctcgtc gcagacggta cgcccatagc
    24961 aagggccact gttgacgtcc acttcgacgt acatctcctg ggttttaatg ccatcagggt
    25021 taatcagata accgatgcag gtggcgtcgt gcaccgggcc gccagccagg ccgtagtttt
    25081 cgaactgcgt tttgagagtg aagttcatga tgtcgctgaa catctctccg gcagggccgc
    25141 ctgccctttc cattcgtgca attacgtccg gggtgcaaac ggtctggttg gtaagatcga
    25201 ggcccatcat cactaatgga acgccggagg tgaacaccac gcgtgcggct tccgggtcgg
    25261 caaagatatt gaactcggca gatggggtga agttgcctgt accgtaagcg ccgcccatta
    25321 gcacaatttc acggattttg ggcaggattg cgggttgcat acgcattgcc accgcgatat
    25381 ttgaaagcgg accaaccggc accagagtga tatcgccatc gcttgccata agggtatcaa
    25441 tgatgtattt caccgcgtgg gtactttctg cctggcgggt cagcggctcg aataccgggc
    25501 catccagtcc ggtttcaccg tgaatattat cggcaacgat ttgttgacgc ataatgggtt
    25561 gcggcatccc cgcataaacc ggaacattaa tctccagttt ctggcaaaca ttcaggccat
    25621 taattaatgt tttatcaagc gtctgattac ccgcaacaat ggtgatacct aataaatcaa
    25681 tcgccggatg tttcgccgcc atcattatag caatagcatc atcatgaccc ggatcacaat
    25741 ccagaataat ttttctcttt tccattgttt atttcctctg tttccagttg cgttattttt
    25801 tctacagcaa cgaaatttcg atcgggccga tgattgaatc ttaacaacag cgtacgaatg
    25861 ctaaatatga gaaatctcat agcggataaa catcgtgaaa gaaatccaca ataatgatct
    25921 taagcagcaa ttgatgagtg aatccgcgtt taaggattgc tttttaacgg atgtttcagc
    25981 cgatacgcgg ctgtttcatt ttttagcgcg tgactacatt gtgcaggaag ggcaacagcc
    26041 gtcctggctg ttttacctga cgcgaggccg cgccaggctt tacgccacgc tagctaatgg
    26101 tcgcgtgtcg ctgctcgatt tctttgccgc cccctgtttt attggcgaga ttgagttaat
    26161 cgacaaagac catgaaccgc gtgcggtgca ggctattgaa gagtgttggt gccttgcgct
    26221 ccctatgaaa cattaccgcc cgctgttatt aaacgacacg ctatttttac gaaaactctg
    26281 cgtcacctta agtcataaaa attatcgtaa tattgtttct ttaactcaga atcaatcatt
    26341 tccgttagtg aatcgcctgg cagcatttat attactctcg caggaaggtg atctttatca
    26401 cgaaaagcat acgcaagcgg cagagtattt aggcgtttct tatcgacatc ttttatatgt
    26461 tctcgcgcaa ttcattcacg acggtttatt aattaaaagc aagaaagggt atctaattaa
    26521 aaacagaaag cagttgtcag gactggcgct ggagatggac ccggagaata aattctccgg
    26581 gatgatgcag taaaaattat tggcaatagc acgattgccg gatgcaacgc ttaataaggc
    26641 gtacatccgg caatgaaact caagctaaac caataaagaa cccggcaatg gtcgcgctca
    26701 tcaggttgga aagcgtcgcc gccgccagcg cccgtaaacc aagctgggcg atttccggcg
    26761 cgcgttttgg cgaaatagcc gaaaacgctc caacgacaac accgatagaa ccaaagtttg
    26821 caaacccaca gagggcgaac gagataatgg caatggtctt gacatccagc gtgccgctcg
    26881 tttgcagata gggcgagaaa ttgagataag caacaaactc attaatcgcc agtttctgcc
    26941 caatcaaact tccggcaaga tttgcatcac tccagtccac ccccataatc catgccaacg
    27001 gggccagcac atagccgaaa atgctttcca gagagacatt ggcaaaacca aaccagccgc
    27061 caactccacc aataatgccg ttgatcagcg caatgatcgc tacaaatgcc attaccaccg
    27121 tcgccacacc ggcggcgatt ttcagcccgg tcattgcacc gttcgccgca gcttcaataa
    27181 tgctttttgg cggtgtttcg ctaaacgaca gattttcaaa cgtaacttgc gaaggttccg
    27241 tcgccggact aagaatacgt gcaaacagga taccgccagg aatagccatc agcgaagcgg
    27301 ctaacaaata gtcaatcggc acccccattc cggcatagcc aatcatcatc gaacccgcaa
    27361 tagacgccat gccgctacaa atagccgtaa atagctcgtt gcgattcatg cgatcaataa
    27421 aaggtttaac gatcgccggg atctcatttt gcccgaggaa aatagtggtg actgcgacaa
    27481 aagattcgat tttgctgatg ttgagggctt tctgaaaaat actgccaaga atacgaatca
    27541 gcagccccat tacgccaatg tagtacagca agctgataag cgcggtaacg aaaataatcg
    27601 ccgggagtac gcgaaaggcg aagataaagc ccgcaccgtc aaacagaaca tccattttgg
    27661 gaccgaccag cgaaccaaaa ataaaggcac ttcctgcgtc actgtaggac atcactttat
    27721 ggacgcctaa tgccgcctgc tctactgccc attttcccgg gggaaaatag agcatgatgc
    27781 caccaatggc aatttgcagc agcagcgcag cgccaaccgt gcgcaagctg atgctctttt
    27841 tatttaccga caacacaaag gcaattgcca gcaataccac catccccgca acacttctca
    27901 ttatatccat aatgattttc ccttcatgcc agtaaacccg gcgtcagcgc caggttttgg
    27961 tatgtttgat gagtacgggc gacggctttc tgcccgccag aaattaaccc acgagacgct
    28021 gatattcttt ggcaatttcg ctcgccagaa tggcgttatt aaagaccagt tgaatattgg
    28081 atttcaggct atcaccgccg gttagctccg caacacgcgc cagcaggaac ggtgtactct
    28141 ctttcccaat aacaccctgt tcttcggctt cagcaacggc ttgatcaatt gctgcattga
    28201 tggtttcttc tggcatcgca aactgttccg ggatcgggtt cgctaccacc aggccgccgt
    28261 tcaaaccggt ttgccacttc actgccatcg cgcgagcaat ttcgcgggca ctgtcaagac
    28321 gaatgctgac ttcaaatggg ctggttcggc agaaaaacgc aggcagcgct tttgtctgat
    28381 aaccaattaa cggtacaccg aaggtttcta aatattctgt tgttaatcct aaatcgagaa
    28441 tagattttgc ccccgcacaa acaacagtga cattggtatt tgccagttcc tgtaaatcgg
    28501 cagaaatatc gaaggtatgt tccgctccgc gatgcacacc accaatacct ccggttgcaa
    28561 aaactttaat cccagccagt gctgcaataa tcatggttga tgcaacggta gtcgcgccat
    28621 ttttccctgc agcaacgaca aaaggtaaat cacgacgact aactttcgtc acgttatgtc
    28681 cttcacggcc cagcaattcg atttcttctt tacttaagcc cactttcatc acaccgccaa
    28741 taatggcgat agtggctggt actgcgccct gtttacgaat tgtttcttct acttcaattg
    28801 cggtttgggc attttgtggg aatggcattc cgtgagaaat tatggtcgat tccagcgcaa
    28861 caaccggttt tttatttttt aaagcatcct gtacttcagc ggatatttgt aataattcag
    28921 gggacagagt taattcagac attctgtgtt ctccactaac gatctaacat tggcaattga
    28981 taattcaggg ttgttggtat attcacaggc aagtgccatt gacgaacatc cctgcgcaaa
    29041 tcgaacagaa tcgataaacg gcataccgtc tacccaacag gaggccagcc ccgccatcat
    29101 ggcgtcgcct gccccagtaa cattgatgac attcgtttta ataggggccg accacccact
    29161 ttcaccgttg atatcactgt agtaaacgcc atcccctccc atgctcagca ccaagcggtt
    29221 caggccgtgt tgatgaaacc aggcggcgac ctttgcgaca tcttcacgtc cggaaagtgc
    29281 aattccgctc aacgtttcgg cttcaagacg gttaggtttt aacgtatgga ttttgctcag
    29341 atgatcgcgg attttgacgc atttccatgc cgataccgga tcaataaata caggcgtttc
    29401 cccgctattt tccagaaccc agaccagcac ctcttcacta aggttgcaat ccgcgacaat
    29461 aacctttgca ccgcgaataa actcctgatg ctgggcaaga aattctgcag aaatacagtc
    29521 actaatactc atatcattta tcgcgacaag catttctcct gtattatcga gcagtgataa
    29581 atagctggat gtattctcac ccggaacaat taagcattta tcgacataaa caccggattg
    29641 attagtttgc gccagtaatg attgcccata aaaatcacca cctactgcac ttaaaagcca
    29701 ggcatttttc cccagtaacg ccagattatg agcgatattc cttcccacgc cacctggggt
    29761 aaatttaatt tttccgggat tcgaatccgc ataattcaat ggatgatgtg aatacccagc
    29821 cacatcaata ttcgccgaac caataattac tatgtagtcc ttttcgcgca tagcacgccc
    29881 cctggcaata aattgccaac gaagaaattg ggtaaaatag ccattacagt caataagact
    29941 attagagcac gtgttcagat ttaaacacat gttcataata agcatattta acgtaatgta
    30001 aatcgatcag aaaaaacgaa atcggccaga tatgaaggcg gtcacaaagt ttgtctattt
    30061 atgcagcaac aaaagagaaa gttgtgatgt gtaacacaca atatggcagg caagaaaaag
    30121 ggcagagatg tttctgccct ccagattgtt gacaacgcgc gctttgttca tcctggatgt
    30181 ggcgtaaacg ccttatccga cctacaaaag cgcaaaaaat tcaatatatt gcagggttca
    30241 tgtaggtctg ataagcatag cgcatcaggc aattttgcgt ttgtcatcat cggggcagag
    30301 atgtctcatc cccgataatg aagataaatt atgcgttttt ttctactatt tgtgtttccg
    30361 ggcgtttcag gaaggcatag gccaaacccg ccaccagcgt accggcaata attgctacca
    30421 ggtaacccaa taccggcgta atagcgccag ggatcagcag aacaaacaga ccaccgtgtg
    30481 gtgccatcag tttcgcacca atcgccattg agattgcgcc agtcagcgcc ccaccaacga
    30541 tacagcacgg tagcacacgc atcggatcac gagcagcaaa cggaattgca ccttccgaaa
    30601 taaagcacag accaagtacc agagcggctt tgccaccttc ctgctgcgct ttgtcgaatt
    30661 tgcgacgcgc caccattgtt gccagaccca ttgccagcgg tggcaccata cctgccgcca
    30721 taatcgccgc catcgggcca taggtttgag tactcagcag acccacaccg aatgcgtacg
    30781 ctgctttgtt taccggaccg cccatgtcag tacacatcat gccaccgagg atcgccccca
    30841 gcagaaccgc attcgcagtc cccatggtct gcagccagtg agtcaaccca tcgagaatgc
    30901 cagcaaccgg tttaccgatt aggtagatca tcgccagacc gaccaccaga ctggaaatta
    30961 gcggaatgat caggatcggt ttcagcgcct ccatactctg tggcagtttc aattgcgtac
    31021 tgattaactt cgcaatgtaa ccagccagga agccagcaat tataccgcca ataaagccag
    31081 aaccggtgct gaccgccagc ataccgccaa tcagacccgg tgtgaggccc ggacgatcgg
    31141 caatggagaa ggcaatataa cctgccagta ccggcaccat cagcgcaaag gctgaaccac
    31201 caccaatctg catcagcgcc gctgccaacg tacccggctc tttaaacgct tcgataccaa
    31261 aagcaaaaga aagcgcgata cacagaccac ctgcaacaac catcggcagc atgtaagaga
    31321 cgcccgtcag caagtgacgg taagcgcctg cactctcttt cttaccttca gtggtcgctg
    31381 tttgagcttt gcccgctggt tcatacggcg ttgcttcagc aaccgcttta tccagttcct
    31441 gcgcagtttt cttcagcgcc agaccggtag aggtgcgata catcggttta ccagcaaatt
    31501 tcgccagatc cacttcgata tctgccgcca caatcaccag atccgctgct gcgacttctt
    31561 caggggtgat tgcattgccc gcgccaacag aaccacgggt ttcaactttc acccaccagc
    31621 cacgtttttt cgcttcggtt tcaatggctt cagccgccat aaaggtgtgt gctacgccag
    31681 tcgggcaagc agtcaccgca actacgcgtt tcggaccgct ggcagcaacc ggtgctgtcg
    31741 cagcgaccgg cgcagtgtaa ggtttcgcat ggcctttggc ttcactcagg aacagctcag
    31801 ggtgcgcaac tgcccgggaa atatcaccca gccagacatt tttaccgttc agcgcgctgt
    31861 cattcgggat ggaatcaccg agaacaatcg ccatttctgc gtcgttcgga ttgtcgatga
    31921 tttccagttt tgcttttcgc gccgccgcgc ccagcagggt cttcgccata taggcgcgtg
    31981 cctgaccgag attagcgtca ataatcagca gcgttttcat tatgcctctc ctgctgtcag
    32041 ttaaaaggtt gtaagtcgac gcgcgccatc attgcggcca actgcggacg atcggtaata
    32101 cccacattgc tttgacttac cgccagggct gcaacagctg tcgccagacg cagtgtgtgt
    32161 tcactggatt cacgcatcag caagccataa atcaggccac caaccataga atcccctgcg
    32221 ccaacggtgc ttacgacatc gactgacggt ggtttggcga tccattcgcc ggaggcatta
    32281 acccaaagcg cgccttcggc acccagtgaa ataacaacat gcgcaatacc ttgttcacgt
    32341 agcgcatgtg cagcttcaat cacatctttc atttcaggca gtttacggcc tgcccagatt
    32401 tccaactcgc ggcggttagg tttcaccagc cacggtgccg ctttcaaacc tgctactaac
    32461 gcttcacggc tactatcaaa gataatgcaa ggacactgac tacgcaggcg agtcatccag
    32521 tcggtgaacg cttccgggct gacgcctgac ggtaagcttc cgctgacaca gaccatatcg
    32581 aactgaccga gccagctcag agaatcagtc acaaagcgtt cccagtcggc gagggtgact
    32641 tcaaaacccg agaagttgaa gtcggtcact tcgccgtctt tttccgtcag cttaacgtta
    32701 attcgggtgc gcccctgtac aacctggaaa cggttggcaa tgcccagctc gctgaacagt
    32761 tgctgaaaac catcctgatt gtctttaccc aggaagccgc caacggtgac atcaattccc
    32821 aggtctttta atactttggc cacgttgatg cctttacccg ccgcatgcag accggtggtt
    32881 ttcaccaggt tcacttcgcc gcgttcaatt tccgggcaga aaccaacaag gtcataagcc
    32941 ggattaaggg tgatcgtagc aacacgtctg ctcattatgc gccctcccca agaccagcag
    33001 cgatagcgtc gccaatggct ttcagcgcct gttcagcatc tgcaccctgg gcggtaaagc
    33061 gtaggcgatg acctttctta acgccaagtg ccacaacttt catcagacta cgtccgtttg
    33121 ccggtttgcc ggtgccatca aggtttgtca cggtaatatc actgttaaat tgtttaatgg
    33181 tattgaccag catggtacct ggacgagcat gcaggccgtg ttcattgcgc accacaaact
    33241 ccgcgcttaa cacgtcgtcg gtcggcgcat catcgctggt cagcagcgcc agcaacgttg
    33301 ccgcatccgc tttcagcaag cggtcagctt tattgtcgag caataaatca gcgagacgct
    33361 taagaaccgc gatgggctga tcgtcattca tcgccacact caccagcatg gctgccgttt
    33421 cgccgtccac atcaaaagca tttgccgcac ggcttaccgc aatcgcgcta cgcagattgc
    33481 cttcggcgct atcgctcagc cagataccct gtccgagatt cagcggttgt tcattgatgg
    33541 ctttggtgac gaaagtggcg tcaactgccc ccacctcttt cagacgcgca gcgttcagcg
    33601 cctgaagagt cagcagatcg ctggcgacga tatccagcgt cagcatttcg ttgtcgagct
    33661 tcagctgctc actctgcttt tcgcccatca gtaatgcgcg aagttcttct gctgttgttg
    33721 ctgacttcag ttgttcagca acggaatcat cgctcagtac gtgggtcagc tggcgtagca
    33781 ggcccagatg ttcatccgag ctggcagcaa taccgattgc cacgtacgct acctgaccgt
    33841 caccccaggt gacgccttcc gggaactgaa atacctgaac gccggttttc agcacctgat
    33901 cgcgggtgtc ggtagtgccg tgtggaatag caataccatt gccgaggaac gttgaggttt
    33961 gctgttcgcg cgccagcatg ccattgacgt agccttctgc tacattaccg gcctgcacca
    34021 gcgccgcagc gacctggcga atcgcctctt ctttgtctcc ggccttttcg cccggatgga
    34081 tgtcctgtac ggataactgg aacatagttc tcctctcttg ctgaattgaa acgattcagc
    34141 ctctatgaga aaaaaagcgc caacctggct tagggttaaa gacaagatcg cgctgaaacg
    34201 tttcaagaaa gcataatact tctgtttcag cacgcaagga aagccgtaaa gttatgagca
    34261 aaagtttgat gtgctgcaca attttttgtc atttttccta attgctgatg ggaaaatccg
    34321 ttatcagcaa tttcatttca gcagcgtcag cagccaatca gcaacagtga aatggcattt
    34381 tgattattcg tggcgctttt gatttcgctg tctgtttatt ttctgacaag cagcgtaaac
    34441 tccgcgtctt cctcttccag tgatcgacct gcatgcataa ctcccccgca gtctccagcg
    34501 cgaaatcgtt tgacctgacc tcgacggcgt ttttaatcgt tgcctttctc accggtattg
    34561 cgggtgctct gcaaaccccg acactcagta ttttccttac cgatgaagta catgcccgtc
    34621 cggcgatggt gggattcttc tttaccggca gcgctgtcat tgggattctg gtaagtcagt
    34681 ttctcgctgg gcgctctgat aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc
    34741 tgttaggcgt gctggcctgc accctttttg cctggaatcg caactacttt gttttgctat
    34801 tcgttggcgt ctttcttagc agctttggct cgaccgctaa cccgcaaatg tttgcccttg
    34861 cccgtgaaca tgccgacaaa accggacgtg aggcggtgat gttcagctct tttttacgcg
    34921 ctcaggtttc actggcatgg gtcattggcc caccgctggc ttatgcgtta gcgatgggtt
    34981 tcagctttac ggtaatgtat ctgagcgcag cggtagcgtt tattgtttgc ggcgtgatgg
    35041 tgtggctgtt tttaccgtcg atgcaaaaag agcttccgct ggcgaccggc acggttgaag
    35101 cgccgcgccg taaccgtcgc gatacgctac tgctgtttgt catttgtaca ttgatgtggg
    35161 gctcgaacag cctgtacatc atcaacatgc cgctatttat tatcaacgaa ctgcatcttc
    35221 ccgagaaact ggccgatgtg atgatgggga ccgccgccgg gctggaaatc ccgacgatgt
    35281 tgattgccgg atatttcgcc aaacgtctgg gtaagcgttt cttaatgcgc gttgctgccg
    35341 tgggtggcgt ctgtttttac gcaggaatgc tgatggcgca ttcacctgtc attctgttgg
    35401 gcttgcagct gctaaatgct atttttattg gcattctggg cggcatcggg atgctctatt
    35461 ttcaggatct gatgcccggt caggcgggtt cagccaccac gctctatacc aacacttcgc
    35521 gcgtgggctg gatcatcgca ggatcagtgg cgggcatcgt cgccgaaatc tggaattatc
    35581 acgctgtgtt ctggtttgcg atggtgatga ttatcgccac tctgttttgc ttactgcgga
    35641 ttaaagatgt ttaaggcgcg gtcagcattt ccagatcgat aagccaggtc atggcctgct
    35701 gacgcgagtt accgcacatt tcagcgctgg cttgtaagcc tgcacagact ttcggtcgca
    35761 gtggcgaggt gaaaatttta cagcgttgct gttcatcaag ctgaatgcag ggcgtattgg
    35821 cgggcttgcc atctggcata ccgggaatag ggctggaaat tgaaggggca gtgcaacacg
    35881 ccccgcaacc cggacggcat tccatacgtt gttctcttaa ggttaaccac cggattgatg
    35941 agcgcgcaca gtaccacctt ttttgcacca gcaaaagtgc gaataccact tgcccgaaag
    36001 gcccgtcgcg agtactttgt cgcgatattt ttgacatttt cgactacagg aatttttcga
    36061 tgccaagagc gaacgaaatt aaaaaaggta tggtactgaa ttacaacggc aaactgctgc
    36121 tggttaagga tattgatatt cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa
    36181 tgcgtttttc tgatgtccgt accgggctga aagtagaaga gcgtttcaaa ggtgatgata
    36241 tcgttgacac cgtgacgctg acccgccgtt acgttgactt ctcctatgtc gatggcaacg
    36301 aatatgtctt tatggataaa gaagactata ccccgtatac cttcaccaaa gatcagattg
    36361 aagaagagtt gctgtttatg ccagagggcg gcatgccgga catgcaggtg ctgacctggg
    36421 acggtcaact gctggcgctt gagctgccgc agaccgttga tctggaaatc gttgaaaccg
    36481 caccaggcat caaaggggca tctgccagcg cccgtaacaa accggcgaca ttgagcactg
    36541 gtctggtgat tcaggtacca gaatacttaa gcccgggcga aaaaattcgt atccatatcg
    36601 aagaacgccg ttatatgggg cgtgctgact aacttcagcc gcatgcagaa aagggatagc
    36661 tcaggctgtc ccttttttaa tttattatac caatcttcta ttttgcgctt cctgaacacc
    36721 ttacgccctc aattttcact cgttgattga tcgccctcac acttcatcgc attaacaatc
    36781 cagaccaatt tcaattgctg tcatataact ttacactgtc attgttaatt tatcgttact
    36841 aagacgtgac tcctatgaag acaattgcct ccgttacgct cccgcatcat gtacacgctc
    36901 cacgctacga tcgccagcag ttgcaatcac gtatcgttca ttttggcttt ggcgcctttc
    36961 accgcgctca tcaggcgtta ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg
    37021 ggatctgtga aatcagcttg ttcagcggtg atcaactgat gagccagctc cgcgcacaga
    37081 accatttata taccgtgctg gagaaaggtg cggacggcaa tcaggtgata attgtcggtg
    37141 ccgttcacga atgccttaat gcaaaactgg attccttagc ggcaattatt gagaaatttt
    37201 gcgagccaca ggtggcaatt gtttccctga cgattaccga aaaaggctat tgtattgacc
    37261 cggccaccgg tgcactcgac accagtaatc cgcggattat tcacgatcta caaacccctg
    37321 aagaacctca ctccgcaccg ggtattctcg tcgaagcact gaaacgccgc cgtgagcgcg
    37381 gccttacacc gtttaccgtg ctctcctgcg acaatattcc cgacaatggc catgtggtga
    37441 aaaacgcggt gctgggaatg gcagaaaaat gttcgccaga actcgccggg tggataaaaa
    37501 aacacgtcag ttttccggga accatggtcg accgcattgt tccggctgca accgacgaat
    37561 cactggcaga aatcagccag catctggggg taaatgatcc ctgcgcgatt agctgcgaac
    37621 cgtttatcca gtgggtggtt gaagataact tcatcgctgg gcgtcctgcc tgggaagtcg
    37681 caggtgtaca aatggtgaat gatgtcctgc catgggaaga gatgaaactg cgtatgctta
    37741 atggcagcca ctcttttctc gcttatctgg gttacctctc aggattcgtc catatcagtg
    37801 attgcatgca ggatcgcgta tttcgccatg ctgccagaac attaatgctg gatgagcaag
    37861 cgccgacact gcaaattaaa gatgtcgatt taacacaata tgcggataag ttaattgcac
    37921 gttttgctaa tccgtcgctg aaacataaga cctggcaaat cgcgatggat ggcagccaga
    37981 aattaccgca acgtatgctg gcaggtattc gcatacatct ggggcgtgaa acggactggt
    38041 cgttgctggc attaggcgtt gctggctgga tgcgttacgt cagcggcgtt gatgatgccg
    38101 gaaatgccat tgatgttcgc gatccgctta gcgataaaat tcgcgaactt gttgcgggca
    38161 gcagcagtga acaacgcgta accgccctgc tttccctgcg tgaagttttc ggtgatgatc
    38221 tgccagataa cccgcatttt gtgcaggcca tcgaacaagc ctggcaacaa atcgtacaat
    38281 tcggcgcaca tcaggcgcta ttaaacaccc tcaaaattta acgatttctg cggttaaagc
    38341 ggatgaagct caccttcgtc cgctctcccc ttctcttttc tgcctttttt agccaggatt
    38401 aacgctcagt taacttacca gagtattgcg gagccgttgt gaccaggacc aacctcatca
    38461 ccggttttct cggcagcggg aaaaccacgt cgattcttca tctgttagcc cataaagatc
    38521 ccaacgaaaa atgggcggta ctggttaatg aatttgggga agtcggaatt gatggtgctt
    38581 tgctcgccga tagcggcgca ttgctgaaag agatccccgg cggctgcatg tgctgcgtta
    38641 atggtttacc catgcaggta gggttgaata ccttactgcg tcagggaaaa ccagaccgct
    38701 tgttgataga gccgaccggg ctgggccatc cgaaacagat cctcgatctg ttaaccgcac
    38761 cagtctatga actgtggata gatctgcgcg ccaccttgtg cattctcgat ccacgcctgc
    38821 tgctggacga aaaaagcgcc agcaatgaaa acttccgtga ccagctggct gccgcagaca
    38881 tcattgtcgc caataaatcc gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgtt
    38941 ggtggcagca aaatggtggc gatcgacaat taattcacag tgagcatggg aaagttgacg
    39001 gtcatcttct ggatttgccg cgtcgcaatt tagccgagtt gcccgccagc gccgcgcatt
    39061 ctcatcagca tgtcgtgaaa aaagggttag cagcgttaag cctgccagag catcaacgct
    39121 ggcgtcgcag tctgaacagc gggcaaggat atcaggcctg cggctggata ttcgacgctg
    39181 atacggtatt cgacaccatt ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg
    39241 tcaaaggcgt gctgcgtatt cccgaagggc tggtacgaat caaccgtcag ggcgatgacc
    39301 tgcacattga aacgcaaaac gttgcgccac cggacagccg tattgagctg atttccagca
    39361 gcgaagctga ctggaatgct ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg
    39421 cgtaaggttg cctgcgtttt tcagtaagat aattagagaa aatatgatta aaaatttgcc
    39481 gcaaatagtg ttgttgaata ttgtcggcct cgcgctgttt ctttcctggt atattcccgt
    39541 taatcatgga ttctggttgc cgattgatgc ggatattttt tatttcttta atcagaaact
    39601 ggtcgaaagt aaggcctttt tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg
    39661 ttgttctctg ctggcgatgg gaatgttgat gctgagtttc tggctgaaag aaaacgcccc
    39721 tggcagacga cgtatcgtga ttatgggtct ggtcatgcta ttaactgcag tggtattaaa
    39781 ccagctgggt caggcattaa ttcctgtaaa acgggccagc ccaacattga cttttaccga
    39841 tattaaccgc gtcagcgaac tgctctctgt tcccacgaaa gatgcctcac gagatagctt
    39901 tcccggcgat cacggcatga tgctgcttat tttttcggca ttcatgtggc gttatttcgg
    39961 caaagttgca ggccttatcg cccttattat ttttgtggtt tttgcatttc ccagagtaat
    40021 gattggcgca cactggttta ctgacatcat tgtcggttcg atgaccgtga tattgatcgg
    40081 tttgccctgg gtgttgctga cgccattaag tgatcgatta atcacctttt ttgacaaatc
    40141 actaccggga aaaaacaaac atttccaaaa caaataactc acagtaatta acatcatcag
    40201 gcttattttt atagtgagga taatcctgat gatgcgcacc gtgctttcat ccatcgcgcg
    40261 caaaaatcat tctctaagta aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa
    40321 ttgaccaatc ccgcttatta actttctgta tcactttttc ttataaaaaa tcatgtaaaa
    40381 ccgctcgcca agaccgcacc aatcgggtaa tctagaactc gttttgcctc ggcggtagat
    40441 tatcctcaca gcatataatt ttgtgcgtta gtccacagat ttggccttaa ggaattgttt
    40501 caacatgccc aggtaattag tctcgtgtcg cttggcattt ttttataacg atatttgtcg
    40561 ttaaggactt caagggaaaa caaacaacat ggtcaaatct caaccgattt tgagatatat
    40621 cttgcgcggg atccccgcga ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa
    40681 caccgcaaag aatatgcatc ctgagacacg tgcagtgggt agtgaaacat catcactgca
    40741 agcttctcag gatgaatttg aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat
    40801 ggatcagtat gctgactgga aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg
    40861 tatcgattgt tctggttttg tacaacgtac attccgtgag caatttggct tagaacttcc
    40921 gcgttcgact tacgaacagc aggaaatggg taaatctgtt tcccgcagta atttgcgtac
    40981 gggtgattta gttctgttcc gtgccggttc aacgggacgc catgtcggta tttatatcgg
    41041 caacaatcag tttgtccatg cttccaccag cagtggtgtt attatttcca gcatgaatga
    41101 accgtactgg aagaagcgtt acaacgaagc acgccgggtt ctcagccgca gctaataaac
    41161 cgtttggatg caatcccttg gctatcctga cgagttaact gaaagcactg cttaggcagt
    41221 gcttttttgt tttcattcat cagagaaaat gatgtttccg cgtcttgatc caggctatag
    41281 tccggtcatt gttatctttt aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt
    41341 catacgcgct cccaattctg gacgtaagct cctgcttacc tgcattgtag caggcgtgat
    41401 gattgcgata ctggttagct gccttcagtt tttagtggcc tggcataagc acgaagtcaa
    41461 atacgacaca ctgattatcg acgtacaaaa gtatctcgat acctattttg ccgacctgaa
    41521 atccactact gaccggcttc agccgctgac cttagatacc tgccagcagg ctaaccccga
    41581 actgaccgct cgcgcggcgt ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa
    41641 aaaaacattc tgttcatctg cgactggtga gatggacatt ccactcaatg aattgattcc
    41701 ggcgctcaac attaataaaa acgtcgatat ggcgatctta cccggcacgc cgatggtgcc
    41761 gaacaaaccc gcaatcgtca tctggtatcg caaccctttg ctgaaaaata gcggtgtgtt
    41821 tgccgctctg aatctcaacc tgacgccttc tctcttttat agttcacggc aggaagatta
    41881 cgatggcgtc gccctcatta ttggcaatac tgcgctatct accttttctt cacgtttgat
    41941 gaacgttaat gaattaaccg acatgccggt ccgtgaaact aaaattgcgg gcattcctct
    42001 gaccgttcgg ctttatgcgg atgactggac atggaacgat gtgtggtacg catttttact
    42061 gggcggcatg agtggaactg tcgttggcct gctctgctat tacctgatga gcgtacgtat
    42121 gcgccccggc agagaaatca tgaccgccat caagcgcgaa caattttacg tggcgtatca
    42181 accggtggtg gatacacaag ctttgcgagt aacgggcctg gaagtactgc tacgctggcg
    42241 gcatcctgtc gcgggagaaa ttcccccgga tgccttcatt aactttgccg aatcgcaaaa
    42301 gatgattgtg ccgctgactc agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt
    42361 agaaaaagtg ctgccagtag gcgtcaaatt tggtattaac attgcgccgg accatctgca
    42421 cagcgaaagc tttaaagcag atatccagaa actgctcact tccctgcccg cacaccattt
    42481 ccagattgtg ctggaaatta ccgagcgcga tatgctgaaa gagcaagaag ccacacaact
    42541 cttcgcctgg ctgcactcgg tcggcgtaga aattgctatt gatgacttcg gcaccgggca
    42601 cagcgcgctt atctatcttg agcgttttac gctcgattat ctgaaaattg accgtggatt
    42661 tatcaacgcc atcggtacgg aaacgatcac ttcacccgta cttgacgcgg tgctgacgct
    42721 ggcgaaacgc ctcaatatgc tgacggttgc tgaaggggtc gaaacgccgg aacaggcgcg
    42781 atggctaagc gaacgcggcg ttaatttcat gcaaggctac tggattagtc gccctttacc
    42841 gctggacgat tttgtccgct ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct
    42901 tacgtccctt attattcata gtgaaagcat gccggattgc ggctaatgat gagtaaaagg
    42961 aaatccattg cagatgattg tgcgcatact gctgctgttt atcgctctgt tcacctttgg
    43021 tgtgcaggcg caggctatca aggaaagcta tgcctttgcc gtgctgggcg aaccccggta
    43081 cgcgtttaat ttcaaccatt ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat
    43141 aacgttgtca gccctcggca ccttcgataa tttcaaccgc tatgcactgc gcggcaaccc
    43201 gggcgcacgc accgagcagc tgtacgacac gctatttacg acttccgatg acgaaccagg
    43261 cagttattac ccgctgattg ctgaaagcgc acgctatgct gacgattatt cctgggtgga
    43321 ggtcgctatt aatccgcgcg cccgttttca tgatggttcg cccattactg cccgcgatgt
    43381 agagtttact tttcaaaaat ttatgaccga aggcgtgccg caatttcgtc tggtctacaa
    43441 aggcaccacc gtcaaagcca ttgcaccgtt aaccgtgcgc attgagttag ctaaacccag
    43501 caaagaagat atgctgagtc tgttttcgct gccgatattt ccagaaaagt actggaagga
    43561 tcacaaactt agcgacccgc tcgccacgcc tccgcttgcc agtggtccgt accgcgttac
    43621 gtcctggaaa atggggcaaa atattgtcta ttcccgcgtg aaagattact gggcagcaaa
    43681 cttaccggta aaccgtggac gctggaattt cgacaccatt cgctacgatt attacctcga
    43741 tgataatgtc gcctttgaag cgtttaaagc aggtgccttt gatttgcgta tggaaaacga
    43801 cgctaaaaac tgggccacgc gttataccgg taaaaatttc gataaaaaat acatcatcaa
    43861 agatgagcaa aagaacgaat cagcccagga tacgcgctgg ctggcgttta atatccaacg
    43921 tccggtattc agcgatcgcc gggtccggga agcaatcact ctcgcctttg actttgaatg
    43981 gatgaacaag gcgttgtttt acaacgcctg gagtcgcacg aacagttatt ttcagaatac
    44041 cgaatacgcg gccagaaatt accccgacgc cgcggagctg gtgcttctgg caccaatgaa
    44101 aaaagatcta ccgccagaag tcttcacaca aatctaccag ccgccggtat ctaaaggcga
    44161 tggctacgat cgtgacaacc tgttaaaagc cgacaaactt ctcaacgaaa cgggctgggt
    44221 gctgaagggt cagcaacgcg ttaatgccac aacgggtcag ccgctcagct ttgaattatt
    44281 gcttcccgca agcagcaata gtcagtgggt attgccgttc cagcacagcc tgcaacggct
    44341 gggtatcaat atggacattc gcaaggtgga taactcgcaa atcactaacc gcatgcgcag
    44401 tcgcgactat gacatgatgc cgcgcctatg gcgggcgatg ccgtggccca gttccgattt
    44461 acagatttcc tggtcatcgg aatatatcaa ttccacttat aatgcccccg gcgtgcaaag
    44521 cccggttatc gactcgctga tcaaccaaat tattgccgcg cagggaaata aagaaaaatt
    44581 actgccgttg gggcgagcac tggatcgcgt attaacgtgg aattattaca tgctgccaat
    44641 gtggtacatg gcggaagacc gtctcgcctg gtgggataaa ttctcccaac ccgctgtacg
    44701 ccctgtttac agcctgggta tcgatacctg gtggtatgac gttaataaag cggccaaact
    44761 gccgtcagcc agacaacagg gagagtagat gggcgcttac ctgattcgcc gtctgttgct
    44821 ggtgatccca acgttatggg ctattatcac tatcaacttt ttcatcgtgc aaattgcgcc
    44881 tggcggtccg gtcgatcagg ccatcgccgc cattgagttt ggtaatgccg gagtattacc
    44941 cggcgcaggc ggtgaaggtg ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag
    45001 cgacagtaat taccgtggcg gacgcggatt agacccagaa gtgatcgctg agatcactca
    45061 tcgctacggt ttcgataagc cgatccacga acgttacttc aaaatgctct gggactacat
    45121 ccgctttgat tttggtgata gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga
    45181 cagtctgccg gtttccatca ccctcggatt gtggagcacg ctgattatct atctggtgtc
    45241 gattccgtta ggcattcgca aagctgttta taatgggagt cgctttgacg tctggagtag
    45301 cgcatttatc atcatcggct acgccattcc ggcctttttg tttgccatcc tgctgattgt
    45361 cttcttcgcg ggcggcagct atttcgacct gttccctcta cgcggcctgg tttccgctaa
    45421 ctttgattcg ctgccgtggt atcagaaaat caccgattat ctgtggcata tcacgctacc
    45481 ggtgctagcg acagtgattg gtggctttgc ggcgctgacc atgctgacca aaaactcatt
    45541 ccttgatgaa gtgcgcaagc aatacgtggt gaccgcgcgt gcgaaagggg taagtgagaa
    45601 aaacattctc tggaaacatg tgttccgcaa cgccatgctg ctggtgattg ccggttttcc
    45661 ggcgacgttt atcagcatgt tttttaccgg ctcgctgctg attgaggtga tgttttcact
    45721 caatggtctt ggcttactgg gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt
    45781 tggtaccttg tatattttca ccctgattgg cctgctgctg aatattgtca gtgatatcag
    45841 ctatacgctg gttgatccgc gtatagattt tgagggacgt taatgtcgcg actcagcccc
    45901 gtcaatcagg cccgttgggc gcgttttcgt cataaccgtc gcggctactg gtcgttatgg
    45961 attttcctcg ttttgtttgg tttgagtttg tgttctgaac ttatcgctaa cgataaaccg
    46021 ttgctggtgc gttatgacgg cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc
    46081 gattttggcg gcccgctggc aagtcaggct gattatcagg acccgtggct gaaacaacgg
    46141 ctggaaaata acggctgggt actgtgggca ccgattcgct ttggtgctac cagtatcaac
    46201 tttgctaccg ataagccctt cccttctcca ccctcccggc aaaactggct gggaacggat
    46261 gccaacggcg gcgatgtgct ggcacgcatt ctctatggca cgcggatctc ggttctgttt
    46321 ggcctgatgc tgactctctg ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc
    46381 tattacggcg gtaaagtcga tctctgggga caacgcttta ttgaagtatg gtcggggatg
    46441 ccgacgctgt ttttgattat tttactttcc agcgtcgtac agcctaactt ctggtggctg
    46501 ctggcaatta ctgtcttgtt tggctggatg agtctggtcg gcgtggtgcg ggcggagttt
    46561 ttacgtactc gtaatttcga ctacattcgt gcggcacagg cgcttggcgt cagcgatcgc
    46621 agtatcatcc tgcgtcatat gttgcctaat gccatggtcg cgaccctcac ctttttaccg
    46681 tttattttat gtagttcgat aaccaccctg acctcgctcg atttcctcgg cttcggtcta
    46741 ccgctcggtt caccgtcact cggtgaactg ctgttacaag ggaaaaataa ccttcaggcc
    46801 ccgtggcttg ggatcaccgc cttcttgtcg gtggcgatat tattgtcttt gctgatcttt
    46861 attggtgaag ccgtccgcga cgcatttgat cctaataagg cggtgtagca tgacgcaaac
    46921 tctgttagcg attgaaaatt tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt
    46981 agtcaatgat gtttcactac agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc
    47041 aggttcaggc aaaagcgtta ccgcgctgtc aattttacgc ctgctccctt ccccgccggt
    47101 tgaatatctc tccggcgata ttcgttttca tggcgaatcg ctgcttcatg ccagcgatca
    47161 aacgttgcgc ggtgtacgcg gtaataagat cgccatgatt tttcaggaac cgatggtgtc
    47221 gttaaatcca ttgcataccc tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg
    47281 gatgcgtcgg gaagcggctc gtggcgaaat tcttaactgc cttgatcgcg tcggtatccg
    47341 ccaggcggca aaacggctga cagattatcc gcatcagctc tccggcggcg aacgccagcg
    47401 ggtgatgatt gcgatggcgc tgttaacgcg accggaatta ttaattgccg atgaaccgac
    47461 caccgcactg gacgtctctg tccaggcgca gattttacag ctgttgcgcg aactgcaagg
    47521 cgagctgaat atgggcatgc tgtttattac tcataacctc agcattgtca gaaaactggc
    47581 ccaccgcgtg gcggtaatgc aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct
    47641 atttgcctca cccactcatc cttacacaca aaagctactc aacagtgaac cgtcaggcga
    47701 tccggtaccg ttgccagaac ctgcctcaac gttgctggat gttgaacagc ttcaggttgc
    47761 cttccccatt cgcaaaggga ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa
    47821 catcagtttt acgctacggg cgggtgaaac actgggttta gtgggcgagt ctggttccgg
    47881 gaaaagcacg acgggactgg cgctgctgcg actgattacc tctcagggca gcatcgtctt
    47941 tgacggtcag ccactgcaag atttaaatcg ccgccagctg ttacctactc gtcatcgtat
    48001 tcaggtggta tttcaggatc caaactcctc gctcaaccca cgactcaacg ttttgcagat
    48061 tattgaggaa ggcttacggg ttcaccagcc gacgctttct gccgcacaac gcgaacaaca
    48121 agtgatagcc gtgatgcatg aagtgggatt agatcctgaa acacgccacc gttatccggc
    48181 ggagttctct ggtggtcagc gacaacgtat tgcgattgcc agggcattaa ttcttaagcc
    48241 ctcactgatc atacttgatg aaccgacatc atcactcgac aaaacggtac aggcgcaaat
    48301 attgacgcta ttgaaatcat tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca
    48361 cgatttgcac gttgtccgcg cgttatgtca tcaggttatc gtactgcgac aaggggaagt
    48421 agtggaacaa ggaccgtgcg cgcgcgtgtt tgccgcaccg cagcaggagt atacgcgtca
    48481 gctactggcg ttgagctgac gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg
    48541 ccacaccaaa atttttcaat cggcatgttg cagcaaactc gtcctggcga tttacaaaca
    48601 ggcacggctc accttcacac tccagcaccg aacatggaac ttcgatgtcg cttaacgcct
    48661 ggcttagttt atacatcacc gaccatgcat tatcaccatc cgggctaagc aatttaagcg
    48721 ccaccaggct gttgtcaccg cacggtccat tttgcggaat cggttcaacc ttcgaacctg
    48781 cgaaaccagc agaccagcga aagttctgcg gtagacgatg gacgattgag agttgtaatg
    48841 aagtcactta ttttccccgg aagcacattt acttcacaat ttatttacat caattttaac
    48901 acatcatcaa caatccgtct ttatacagat cgtaaagatt gatgcctcgc ctgcatggcc
    48961 tctatggctc tgatttaagt aatatctgcg ggcgtggtca cgtttgcggg gctatatgta
    49021 cccgtttctg cgatctaact caaccttttt aactacaatg atgtgacttt ttacataaat
    49081 tgattttaca taaaataaac atatatcggg ggaatgatag atttgtggtt gacatgcatc
    49141 aacaaaggaa gccttttagc ttcctcgttg tgcaatagat caccgttttt tcggtcgact
    49201 ggcgtacaga cagaagagaa tggagctggt tgcgcagaat gcaattgacc aaatcatcgg
    49261 ccatgcagag ttaaaggtcg caagagaaag caatgcgcca acaattgccc ctatgccaaa
    49321 acggaaggtt cctgccagcg aagatgccgt tcccgccata tggggaaact catcaagaat
    49381 gaccgccatc gcattggatg acaccataga cacgcagccc acaaacgccg caacgccaac
    49441 caccagcgac cagaatccca gccccagcag cgcactgatg accatccacg ctgccataat
    49501 aaattgtatc cacaaccccg agcggaacat atttaacgcg ccaatgcggc ggacgaagcg
    49561 gctgttaaag atggtcatca cgaacagaaa aacaatgttt agcgcaaagt aataaccaaa
    49621 gttttccggt gcaacgtggt tgatttcaat gtagacaaac ggtccggcgc tcaggaacga
    49681 gaacattccg gcaaagctga aaccgctggc cagcatataa ctcaggacac gcttatgacg
    49741 gaacagcgcc gcaaagttac caatagtggt acgaatgtga aaaggctggc gaggatccag
    49801 aagatgtaat gccagctcag ccacaccagc acccagccgc caactatcgg tgccatcagc
    49861 ggtgcaatgg tcgtcaccag catgacaaac gacatcatcc gcgagaactc ttctttcggg
    49921 taaatatcac gcatcaaagc gttgataacc acgctggccg cagccgcagc cagcccgtgg
    49981 aagaaacgca tcacaatcag ctgatcgatg gtttgtgcca acgcacacgc caccgcggcg
    50041 gcggcaaaca ccagcgtacc gccaagaacc accggcttac gcccgaagct gtctgccatc
    50101 ggcccgtaga ttaactgccc caacgcaaag cccagaatat aagtactcag ggtcatctgc
    50161 gtactgcccg ccggtacgcc aaactgcgct gaaattaccg gtagcgcggg cagatacata
    50221 tcaatcgaca gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac
    50281 gaatgctgtc gggtggtcac aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg
    50341 gcaatttctt cttcagttaa cggacgatat tcaccggggg ctaaatcagc atccagcgta
    50401 atgccgccaa tacgttcacg atgcagctca accacatggt tacccacggc ggcgaacatg
    50461 cgtttcacct gatgataacg cccttcgctg atggtcagac gaacctgcgt tggggtaatc
    50521 acttccagca ccgccggctt agtgagatct ttttcgttat gcagctgcac gcctttagca
    50581 aattgttctg ccgtatcgtc agctacgggt gataccagcg tcaccagata ggtcttctcg
    50641 caatgatggc gcggagaagt aatgcggtgc gaccactgac catcatctgt cattagtacc
    50701 agaccggtgg tatcgatatc cagccgccct gcggcgtgca gtttccacgc taccggttca
    50761 tctagaaaat agagcaccgt tgggtgatca gggtcgtccg tggagcaaac ataaccctga
    50821 ggcttattga gcatgaagta acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa
    50881 gcgacatcat gttcaggaag cagtttgaac gctgcattac ggacgatttc gccatcgacg
    50941 gtgacacgat tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt
    51001 gcgataaatt tatcaagtcg catgtgtgtg attttgcctg taaaaaacgg aggtcgggta
    51061 atgacccgaa tatctgaact gttgtctgcc cagtatagcg gtctaataac atccctcaag
    51121 ggaaaaagat ccgtggcata ctattagcag aataatctac ctacgcgaga ccatgatttt
    51181 tacacttcgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca
    51241 taaaacccct gccgttatcg tgctgcccac cggtgcaggt aaaagcctgg tgatagcgga
    51301 actggcacgg ctggctcgtg gtcgcgtact ggtgctggca cacgttaaag aactggtggc
    51361 gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg ccgccgggct
    51421 gaagcgtaaa gagagccacg gtaaagtggt atttggcagt gtgcagtcgg tcgcccgtaa
    51481 tattgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgtc accgtattgg
    51541 tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgacaaaag tgaatcccca
    51601 cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggtt ggatctacca
    51661 gttccattat cacggcatgg tacgcagcga tgagaaagcc cttttccgtg actgcattta
    51721 tgagctgccc ctgcgttata tgattaaaca cagctatctg acgccgccag aacgactgga
    51781 tatgccagta gtgcaatatg atttcagccg cttgcaggca cagagcaacg ggctgttcag
    51841 cgaagccgat ctcaaccgtg agctgaaaaa acagcaacgt attaccccgc acatcatcag
    51901 ccagattatg gagttcgccg aaaagcgtaa aggagtgatg attttcgccg cgacggtcga
    51961 acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga ttactggcga
    52021 cacccccggc gctgagcgcg atgtgttaat tgaaaatttt aaagcccagc gttttcgcta
    52081 tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc
    52141 cattctgcgc cctaccgaat cagtgagtct ttaccaacaa attgtcgggc gcggtctgcg
    52201 tctcgctccg ggcaagactg attgtttaat tcttgattat gcgggtaatc ctcacgatct
    52261 ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgttccgg tacaggtttt
    52321 ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg acgggacatt
    52381 gattgaacac tttggtcgcc gctgtcaggg atggtttgaa gatgacgacg gtcatcgcga
    52441 acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat
    52501 tgccgctcgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg atatgttaaa
    52561 agcggcgcta cgactgaaag atgcgctggt attacgctgt agcggcatgt ctttgcagca
    52621 tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag acggtgcgga
    52681 tgtgagtgag cgtttccgtc tgcaaacacc tgcccagcgt accgccttcg agcagctttt
    52741 tatccgcccg catacgcgca caccgggcat cccgctgcgc tggatcaccg ccgccgatat
    52801 cctcgcccag caagccttat tgcgaccccc ggattttgtc gtcgcccgca tgaaaggcca
    52861 gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga
    52921 attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata gaatctcacc
    52981 cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt agcaggatta
    53041 atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag cagggtaagg
    53101 gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac ggtggcaaag
    53161 aagcgccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa gctaaagctg
    53221 aattctacag cgaagttctg accatcgttg ttgacggtaa agaaatcaaa gttaaagctc
    53281 aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc gttcgcgctt
    53341 aattgctgaa taagttgtaa aaaaccccgc tcccgcgggg ttttttgtat ctgcagatta
    53401 tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg ccgcatctgg
    53461 cattcagaac acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa ttggtcgcgc
    53521 aaattcggcg gcgtgccttt aatggtcagg gtatcggtcg ccggctccca gaagatgcgt
    53581 tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc ggcaaacttc
    53641 gtcaactgac gcagtgtgct ccggtctgcc gggaagctct cttccagttc gtagcctttt
    53701 tcagccgcaa actccgtgaa gctcacttcg cttacgcctg ccagctcttt cgacagcgat
    53761 tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata aacctgctgg
    53821 cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc atcaaccgct
    53881 tgcaacagcc cacggttttg tgctttcgtg ttcaatcctt cgctggcccc gaggaaatcc
    53941 ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag atagcgggtg
    54001 gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc cgcatgattg
    54061 atatcaagat aatgggttgg gttgatgtcg agattttcgt taacacgcat actgctcagg
    54121 ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg gcaaaacagc
    54181 acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg accagttgcc
    54241 gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc
    54301 agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata gacccgatgc
    54361 agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc gcgcaacacc
    54421 agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg gttgatatcc
    54481 agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc ggttaagcga
    54541 cgcaataaaa gatggcggtc attttacaga aggcagcgta acgagaatga tttaacgcat
    54601 aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc aactaactga
    54661 tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca attgctcgca
    54721 gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct gatggtgtta
    54781 ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg ccaggcaatt
    54841 gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc gcactaaggg
    54901 aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag tctcccagat
    54961 ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg tcattggcag
    55021 ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt attcctacgt
    55081 aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga tcctcttccc
    55141 gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca ttctggcaac
    55201 ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc atctccatct
    55261 taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg cgcgcgactg
    55321 gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt ttgcgacgtg
    55381 gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc cgctggccgc
    55441 attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg atgccaactt
    55501 ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga tgacggcgcg
    55561 acgttttctt gagaagcatg gtctgcttga tgcgcaggag tatcaacgcc gtcttattga
    55621 gcaaggtaat ccagacgccg tttccgttca gtatccgtta agcgaactgc gctatcgcga
    55681 tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact actcacgctt
    55741 cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca
    55801 tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg gtatctcgcc
    55861 gagctatatg gacggcattc tgtcgacccg tacgcctgcg gcattaatta ctgcgcttaa
    55921 tcagcaaggc tatcagctgg ggttattctc atcagatggc tttaccagcc cgctgtatcg
    55981 ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc acccaatccg acgagcagac
    56041 cgccacgcag tggatcaact ggctgggcca ctacgcacaa gaagataacc gctggttctc
    56101 gtgggtttct ttcaatggca ctaacattga cgacagcaat cagcaggcat ttgcacggaa
    56161 atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca atgcactgcg
    56221 tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg gtattccgct
    56281 gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc cattagtgat
    56341 tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata ccgatctgat
    56401 gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat attcgcaagg
    56461 tcaggatttg ttcaaccctc aacgccgtca ttactgggtt actgcagcgg ataacgatac
    56521 gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta aataccgcac
    56581 ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt tgttattgca
    56641 agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc agttagcgaa
    56701 atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag tcggcacgta
    56761 gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt
    56821 gccgaccaaa aatcccaaga aaaaaccaac ctttacggtt ggttttttta tatctgcaat
    56881 taatttgata aacagaccgt gacacatcac agcctgttta ttttctgtta tcagaacgtc
    56941 cagaccactc ccgcctgagt attccacggc gattctaccc ctgcgccatt gccatagctg
    57001 actgacaaat gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg
    57061 ccacgggttc cagccacatc attattaaat ttgccatcgt cattaacttt cacatggtta
    57121 gaatcggcgt attcctgacg cacggcggct ttcagccagg gttccaacgt cgtaccgttt
    57181 tgcaggtcca tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt
    57241 cccacatccg cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg
    57301 gtaaaggcga gatagggtct aacactccac aatccgtcaa cccaacggaa cccgctctca
    57361 acatgagcac ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc
    57421 ttgccatgga tggtgttggc aaaacggtca actttcacca ccccatcaac ataggcaccg
    57481 ttctgatgct cccaaccggc ataagccccc agggtgtagc tatcaatatt gcctttgccg
    57541 ccgcgatcaa aaccaatatc agaatgagag taatcaaaga acaagccgcg aattgtgctg
    57601 ctttcttcac gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca
    57661 aagccagctc ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc
    57721 gccgtatcgt aattaacgcc ttttacgcta ccaagacgct cacgcacggt gtccagttct
    57781 gcatcaaata ccagcggttg tgctgctgcc atgttcagca catcagtggt tgaaggggta
    57841 atttgcgcgc gattctctgc gaggctccag ctatggttgc cattatccag caaggtatat
    57901 tcatacgtac cgatatcaac aacgcctccg gcattgccca acgtgaatgc agcatcaccg
    57961 ccgcccgtcg ttaccagtgt aaggctatct cctgctgccg ggctggcccc cgtgtccgtc
    58021 acgaatattt tgaaatcacc tgttgcctga ccgatgacgt tgagctgatc gctctgatga
    58081 ttagccatat cggtacgcat ataaaattgc cgtttcccga cagggtattg gtggtcaacg
    58141 taatgaaatt ttccgcactt gccgtcacag atgaacgagt cactggctca gccatcataa
    58201 tcacatttgc gccggacatc gcaaggttgg ctaagttgag gcgagtgttt tggttcgtcg
    58261 tagtgatatc ggcaatgatg gtccatgcgc tattttcgag cgaaacgtcc gcttggctgg
    58321 tatccacggc accatgcgtt ctaaaagaag cgccctcaga aaccactacc ttacccacca
    58381 acatagcggg tgcataattt attttcggct tcatgacctc aagaatgcca tcattccctc
    58441 tgactgacac gttaaccatt gtgccagccc agacattagc gcggccattg ttgataacca
    58501 tattttcaga gatggcttta gaggaatacg tgatacttcc attctgatct gatcgaccga
    58561 gatcatatac agcatttgcc tgcactttcg taccagtatc ctttcccagc gattgcatag
    58621 tggcatgctc atcaaggata gtgtcaatgg cctgagtgtc ctccataaca atgagcccgg
    58681 agccatcatc cagttcataa ttttttgaca caccatcctt tatactaaat tggcctttac
    58741 tgtttgtacc actcacttcc agcgcattcg ttgagacaat taatttcccg cctgcttttt
    58801 tatcaacgcc agttgcagtc ccgccatcca taacctccag taatcctccc ttttcgatag
    58861 cggcaaagac ggcaatccca tctttttcaa caattaagcg cccgccttta atcgtgggat
    58921 cattcgccgc g
//
LOCUS       DAZOXR010000005        57430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZOXR010000005 DAZOXR010000000
VERSION     DAZOXR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.005"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHUNN_000254"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHUNN_000254"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0574744.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3122)
                     /locus_tag="ACHUNN_000255"
                     /pseudo
     CDS             complement(1496..3122)
                     /locus_tag="ACHUNN_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(3581..3667)
                     /locus_tag="ACHUNN_000256"
     tRNA            complement(3581..3667)
                     /locus_tag="ACHUNN_000256"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Leu,seq:gag)
     gene            complement(3682..4014)
                     /gene="secG"
                     /locus_tag="ACHUNN_000257"
     CDS             complement(3682..4014)
                     /gene="secG"
                     /locus_tag="ACHUNN_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0574745.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="ACHUNN_000258"
     CDS             complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="ACHUNN_000258"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0574746.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5572..6420)
                     /gene="folP"
                     /locus_tag="ACHUNN_000259"
     CDS             complement(5572..6420)
                     /gene="folP"
                     /locus_tag="ACHUNN_000259"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0574747.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="ACHUNN_000260"
     CDS             complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="ACHUNN_000260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0574748.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="ACHUNN_000261"
     CDS             complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="ACHUNN_000261"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0574749.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9299..9592
                     /gene="yhbY"
                     /locus_tag="ACHUNN_000262"
     CDS             9299..9592
                     /gene="yhbY"
                     /locus_tag="ACHUNN_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0574750.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9748..10224)
                     /gene="greA"
                     /locus_tag="ACHUNN_000263"
     CDS             complement(9748..10224)
                     /gene="greA"
                     /locus_tag="ACHUNN_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0574751.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10472..11905
                     /gene="dacB"
                     /locus_tag="ACHUNN_000264"
     CDS             10472..11905
                     /gene="dacB"
                     /locus_tag="ACHUNN_000264"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0574752.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11960..12067
                     /locus_tag="ACHUNN_000265"
                     /pseudo
     CDS             11960..12067
                     /locus_tag="ACHUNN_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="ACHUNN_000266"
     CDS             complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="ACHUNN_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0574753.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13279..14244)
                     /locus_tag="ACHUNN_000267"
     CDS             complement(13279..14244)
                     /locus_tag="ACHUNN_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0574754.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="ACHUNN_000268"
     CDS             complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="ACHUNN_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0574755.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="ACHUNN_000269"
     CDS             complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="ACHUNN_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0574756.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15219..16190
                     /gene="ispB"
                     /locus_tag="ACHUNN_000270"
     CDS             15219..16190
                     /gene="ispB"
                     /locus_tag="ACHUNN_000270"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0574757.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHUNN_000271"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHUNN_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0574758.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHUNN_000272"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHUNN_000272"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0574759.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHUNN_000273"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHUNN_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0574760.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHUNN_000274"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHUNN_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0574761.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHUNN_000275"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHUNN_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0574762.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHUNN_000276"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHUNN_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0574763.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHUNN_000277"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHUNN_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0574764.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHUNN_000278"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHUNN_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0574765.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="ACHUNN_000279"
     CDS             21785..22762
                     /locus_tag="ACHUNN_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0574766.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHUNN_000280"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHUNN_000280"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0574767.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHUNN_000281"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHUNN_000281"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0574768.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="ACHUNN_000282"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="ACHUNN_000282"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0574769.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="ACHUNN_000283"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="ACHUNN_000283"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0574770.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="ACHUNN_000284"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="ACHUNN_000284"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0574771.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHUNN_000285"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHUNN_000285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0574772.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="ACHUNN_000286"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="ACHUNN_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0574773.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHUNN_000287"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHUNN_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0574774.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHUNN_000288"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHUNN_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0574775.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="ACHUNN_000289"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="ACHUNN_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0574776.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHUNN_000290"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHUNN_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0574777.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHUNN_000291"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHUNN_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR0574778.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHUNN_000292"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHUNN_000292"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0574779.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHUNN_000293"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHUNN_000293"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0574780.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="ACHUNN_000294"
     CDS             complement(34631..35560)
                     /locus_tag="ACHUNN_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0574781.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTFTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="ACHUNN_000295"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="ACHUNN_000295"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0574782.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="ACHUNN_000296"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="ACHUNN_000296"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0574783.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42714
                     /locus_tag="ACHUNN_000297"
                     /pseudo
     CDS             42310..42714
                     /locus_tag="ACHUNN_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42806..43111
                     /locus_tag="ACHUNN_000298"
     CDS             42806..43111
                     /locus_tag="ACHUNN_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0574784.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHUNN_000299"
     CDS             complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHUNN_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0574785.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHUNN_000300"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHUNN_000300"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0574786.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="ACHUNN_000301"
     CDS             complement(44520..45209)
                     /locus_tag="ACHUNN_000301"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0574787.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHUNN_000302"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHUNN_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0574788.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHUNN_000303"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHUNN_000303"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0574789.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHUNN_000304"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHUNN_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0574790.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="ACHUNN_000305"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="ACHUNN_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0574791.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="ACHUNN_000306"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="ACHUNN_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0574792.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="ACHUNN_000307"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="ACHUNN_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0574793.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="ACHUNN_000308"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="ACHUNN_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0574794.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="ACHUNN_000309"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="ACHUNN_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0574795.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="ACHUNN_000310"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="ACHUNN_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0574796.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /gene="zapG"
                     /locus_tag="ACHUNN_000311"
     CDS             54320..54718
                     /gene="zapG"
                     /locus_tag="ACHUNN_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0574797.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="ACHUNN_000312"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="ACHUNN_000312"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR0574798.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="ACHUNN_000313"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="ACHUNN_000313"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0574799.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14264 a        15302 c        14254 g        13610 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
     3181 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
     3241 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
     3301 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
     3361 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
     3421 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
     3481 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
     3541 aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
     3601 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
     3661 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
     3721 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
     3781 ggttttgttg ctattgatgt tacccagcac cagactgatg ataaagaata acgttgccag
     3841 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
     3901 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
     3961 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
     4021 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
     4081 tctttcccac tgaagtgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
     4141 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
     4201 aaggcgacaa tcgccgcctt tttagccagt tatttaccgt tttaaacggc ttttactgca
     4261 tcggcgatac ggtgtgcaaa ttcagttacc tgcgcttcgt cttcaccttc taccatcacg
     4321 cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc
     4381 gcttcaactt cggcggtcac agctttaact gactcatgct caagcggatc gccgctacct
     4441 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
     4501 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
     4561 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
     4621 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
     4681 ggaattccta actgtttcaa cgccagctca agccccatgt tactcatcaa tgtacccaca
     4741 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
     4801 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
     4861 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
     4921 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
     4981 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
     5041 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
     5101 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
     5161 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
     5221 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
     5281 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
     5341 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
     5401 gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt acgggagccg
     5461 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
     5521 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
     5581 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
     5641 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
     5701 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
     5761 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
     5821 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
     5881 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
     5941 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
     6001 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
     6061 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
     6121 tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
     6181 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
     6241 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
     6301 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
     6361 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
     6421 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
     6481 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
     6541 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
     6601 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
     6661 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
     6721 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
     6781 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
     6841 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
     6901 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag gttggtcgca
     6961 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
     7021 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
     7081 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
     7141 gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
     7201 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
     7261 cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
     7321 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc accggagaaa
     7381 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
     7441 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
     7501 cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg gttagtcgcg
     7561 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
     7621 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
     7681 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
     7741 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
     7801 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta
     7861 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
     7921 ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc gtcgcagccc
     7981 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
     8041 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccac
     8101 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
     8161 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
     8221 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
     8281 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
     8341 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
     8401 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
     8461 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
     8521 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
     8581 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
     8641 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
     8701 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg
     8761 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
     8821 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
     8881 ccctgaagaa agtctacacc aacgatagga tccataggta aaagatcgca agcgatgatg
     8941 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
     9001 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
     9061 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
     9121 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
     9181 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
     9241 atatgggaga tggcggtaga atgacccatt ttcaatccca acgtaagcaa aaaatacgat
     9301 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
     9361 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
     9421 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
     9481 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
     9541 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
     9601 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
     9661 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
     9721 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
     9781 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
     9841 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
     9901 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
     9961 aaaaataacg cgcccattgt taggcatttt ggtgacatca atcacctgcg cgtttgacag
    10021 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    10081 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    10141 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    10201 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    10261 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    10321 aaaaataccg acccaggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt
    10381 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    10441 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    10501 gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact
    10561 ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat
    10621 tgattaccac agtcagcaga tggcgctgcc tgccagtacc cagaaagtga ttactgcgct
    10681 ggcggcgttg attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg
    10741 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    10801 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    10861 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    10921 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga
    10981 ccgcaactgt ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat
    11041 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    11101 ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac
    11161 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    11221 tggagccagt tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    11281 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    11341 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg ataacatgat
    11401 cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtacctg gtacatggcg
    11461 ggccgggtcg gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa
    11521 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    11581 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    11641 gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    11701 ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    11761 cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt
    11821 agaacctgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    11881 taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggcttca
    11941 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    12001 acaaaatcgt gcaaattcaa catattgcaa ttctcttgta ggcctgataa gcgtagcgca
    12061 tcaggctgat ttggcgttta tcatcagtga ttaacgcttg taaatgaact caacgccttc
    12121 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    12181 aagctgctgg cgatgataat cgtcccacat gaattcgact ttctctggct gtttcgcttc
    12241 ttcagcctgc acgaccgggt tttcaaggat aaaggtcatc acatcccagc agagatcttt
    12301 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    12361 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
    12421 cactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    12481 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    12541 caggacgcgg caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc
    12601 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    12661 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    12721 cgccgatacc gcgcgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    12781 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt
    12841 tttctgccgt ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca
    12901 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc
    12961 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    13021 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    13081 aaaacgataa tcgataagcg tgttcaggtt ttcgtcggct tccatccata cgtcaccacc
    13141 atcaccaccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    13201 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    13261 cattttattc tccataaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    13321 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    13381 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    13441 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    13501 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt
    13561 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    13621 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
    13681 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    13741 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    13801 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
    13861 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    13921 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    13981 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    14041 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    14101 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    14161 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
    14221 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    14281 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    14341 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    14401 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    14461 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    14521 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
    14581 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    14641 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatgaccc
    14701 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    14761 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    14821 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    14881 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    14941 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    15001 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    15061 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
    15121 ccattttttc agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc
    15181 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
    15241 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
    15301 acgtccaact gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
    15361 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
    15421 ccattgctgc cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg
    15481 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca
    15541 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
    15601 cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt catcgcagaa ggtgaagttc
    15661 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
    15721 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
    15781 gtacgccgga ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
    15841 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
    15901 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
    15961 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
    16021 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
    16081 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
    16141 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
    16201 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
    16261 tataacacct gaaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcattttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgccg cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggtg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttgg
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc aaactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcagaaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcaacaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgaacgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacaca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagtt ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg
//
LOCUS       DAZOXR010000006        47770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZOXR010000006 DAZOXR010000000
VERSION     DAZOXR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.006"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            54..686
                     /gene="comR"
                     /locus_tag="ACHUNN_000314"
     CDS             54..686
                     /gene="comR"
                     /locus_tag="ACHUNN_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0574800.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(748..1287)
                     /locus_tag="ACHUNN_000315"
     CDS             complement(748..1287)
                     /locus_tag="ACHUNN_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0574801.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHUNN_000316"
     CDS             complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHUNN_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0574802.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3226..3768)
                     /gene="ycfP"
                     /locus_tag="ACHUNN_000317"
     CDS             complement(3226..3768)
                     /gene="ycfP"
                     /locus_tag="ACHUNN_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0574803.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3791..4816)
                     /gene="nagZ"
                     /locus_tag="ACHUNN_000318"
     CDS             complement(3791..4816)
                     /gene="nagZ"
                     /locus_tag="ACHUNN_000318"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0574804.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4827..5651)
                     /gene="thiK"
                     /locus_tag="ACHUNN_000319"
     CDS             complement(4827..5651)
                     /gene="thiK"
                     /locus_tag="ACHUNN_000319"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0574805.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5632..6273)
                     /gene="lpoB"
                     /locus_tag="ACHUNN_000320"
     CDS             complement(5632..6273)
                     /gene="lpoB"
                     /locus_tag="ACHUNN_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0574806.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6287..6664)
                     /locus_tag="ACHUNN_000321"
     CDS             complement(6287..6664)
                     /locus_tag="ACHUNN_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0574807.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6667..7026)
                     /gene="hinT"
                     /locus_tag="ACHUNN_000322"
     CDS             complement(6667..7026)
                     /gene="hinT"
                     /locus_tag="ACHUNN_000322"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0574808.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7360..9549
                     /gene="fhuE"
                     /locus_tag="ACHUNN_000323"
     CDS             7360..9549
                     /gene="fhuE"
                     /locus_tag="ACHUNN_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR0574809.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9609..11042)
                     /gene="ptsG"
                     /locus_tag="ACHUNN_000324"
     CDS             complement(9609..11042)
                     /gene="ptsG"
                     /locus_tag="ACHUNN_000324"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0574810.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11337..12134)
                     /locus_tag="ACHUNN_000325"
     CDS             complement(11337..12134)
                     /locus_tag="ACHUNN_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0574811.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12145..13149)
                     /gene="holB"
                     /locus_tag="ACHUNN_000326"
     CDS             complement(12145..13149)
                     /gene="holB"
                     /locus_tag="ACHUNN_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0574812.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13146..13787)
                     /gene="tmk"
                     /locus_tag="ACHUNN_000327"
     CDS             complement(13146..13787)
                     /gene="tmk"
                     /locus_tag="ACHUNN_000327"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0574813.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13777..14799)
                     /gene="yceG"
                     /locus_tag="ACHUNN_000328"
     CDS             complement(13777..14799)
                     /gene="yceG"
                     /locus_tag="ACHUNN_000328"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0574814.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14802..15611)
                     /gene="pabC"
                     /locus_tag="ACHUNN_000329"
     CDS             complement(14802..15611)
                     /gene="pabC"
                     /locus_tag="ACHUNN_000329"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0574815.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15731..16972)
                     /gene="fabF"
                     /locus_tag="ACHUNN_000330"
     CDS             complement(15731..16972)
                     /gene="fabF"
                     /locus_tag="ACHUNN_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0574816.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17060..17296)
                     /gene="acpP"
                     /locus_tag="ACHUNN_000331"
     CDS             complement(17060..17296)
                     /gene="acpP"
                     /locus_tag="ACHUNN_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0574817.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17507..18241)
                     /gene="fabG"
                     /locus_tag="ACHUNN_000332"
     CDS             complement(17507..18241)
                     /gene="fabG"
                     /locus_tag="ACHUNN_000332"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0574818.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18254..19183)
                     /gene="fabD"
                     /locus_tag="ACHUNN_000333"
     CDS             complement(18254..19183)
                     /gene="fabD"
                     /locus_tag="ACHUNN_000333"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0574819.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19199..20152)
                     /gene="fabH"
                     /locus_tag="ACHUNN_000334"
     CDS             complement(19199..20152)
                     /gene="fabH"
                     /locus_tag="ACHUNN_000334"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0574820.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20220..21290)
                     /gene="plsX"
                     /locus_tag="ACHUNN_000335"
     CDS             complement(20220..21290)
                     /gene="plsX"
                     /locus_tag="ACHUNN_000335"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0574821.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21371..21544)
                     /gene="rpmF"
                     /locus_tag="ACHUNN_000336"
     CDS             complement(21371..21544)
                     /gene="rpmF"
                     /locus_tag="ACHUNN_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0574822.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21596..22117)
                     /gene="yceD"
                     /locus_tag="ACHUNN_000337"
     CDS             complement(21596..22117)
                     /gene="yceD"
                     /locus_tag="ACHUNN_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0574823.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22316..22900
                     /gene="yceF"
                     /locus_tag="ACHUNN_000338"
     CDS             22316..22900
                     /gene="yceF"
                     /locus_tag="ACHUNN_000338"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0574824.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23012..23971)
                     /gene="rluC"
                     /locus_tag="ACHUNN_000339"
     CDS             complement(23012..23971)
                     /gene="rluC"
                     /locus_tag="ACHUNN_000339"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0574825.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24544..27729
                     /gene="rne"
                     /locus_tag="ACHUNN_000340"
     CDS             24544..27729
                     /gene="rne"
                     /locus_tag="ACHUNN_000340"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0574826.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27924..28877)
                     /gene="flgL"
                     /locus_tag="ACHUNN_000341"
     CDS             complement(27924..28877)
                     /gene="flgL"
                     /locus_tag="ACHUNN_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR0574827.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28889..30532)
                     /gene="flgK"
                     /locus_tag="ACHUNN_000342"
     CDS             complement(28889..30532)
                     /gene="flgK"
                     /locus_tag="ACHUNN_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR0574828.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30598..31539)
                     /gene="flgJ"
                     /locus_tag="ACHUNN_000343"
     CDS             complement(30598..31539)
                     /gene="flgJ"
                     /locus_tag="ACHUNN_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0574829.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31539..32636)
                     /gene="flgI"
                     /locus_tag="ACHUNN_000344"
     CDS             complement(31539..32636)
                     /gene="flgI"
                     /locus_tag="ACHUNN_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0574830.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32648..33346)
                     /gene="flgH"
                     /locus_tag="ACHUNN_000345"
     CDS             complement(32648..33346)
                     /gene="flgH"
                     /locus_tag="ACHUNN_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0574831.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33399..34171)
                     /gene="flgG"
                     /locus_tag="ACHUNN_000346"
                     /pseudo
     CDS             complement(33399..34171)
                     /gene="flgG"
                     /locus_tag="ACHUNN_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34343..35098)
                     /gene="flgF"
                     /locus_tag="ACHUNN_000347"
     CDS             complement(34343..35098)
                     /gene="flgF"
                     /locus_tag="ACHUNN_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR0574832.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35118..36323)
                     /gene="flgE"
                     /locus_tag="ACHUNN_000348"
     CDS             complement(35118..36323)
                     /gene="flgE"
                     /locus_tag="ACHUNN_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0574833.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36348..37043)
                     /gene="flgD"
                     /locus_tag="ACHUNN_000349"
     CDS             complement(36348..37043)
                     /gene="flgD"
                     /locus_tag="ACHUNN_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0574834.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37055..37459)
                     /gene="flgC"
                     /locus_tag="ACHUNN_000350"
     CDS             complement(37055..37459)
                     /gene="flgC"
                     /locus_tag="ACHUNN_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR0574835.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37463..37879)
                     /gene="flgB"
                     /locus_tag="ACHUNN_000351"
     CDS             complement(37463..37879)
                     /gene="flgB"
                     /locus_tag="ACHUNN_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0574836.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38034..38693
                     /gene="flgA"
                     /locus_tag="ACHUNN_000352"
     CDS             38034..38693
                     /gene="flgA"
                     /locus_tag="ACHUNN_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0574837.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38769..39062
                     /gene="flgM"
                     /locus_tag="ACHUNN_000353"
     CDS             38769..39062
                     /gene="flgM"
                     /locus_tag="ACHUNN_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0574838.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            39067..39483
                     /gene="flgN"
                     /locus_tag="ACHUNN_000354"
     CDS             39067..39483
                     /gene="flgN"
                     /locus_tag="ACHUNN_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0574839.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39523..41058)
                     /gene="murJ"
                     /locus_tag="ACHUNN_000355"
     CDS             complement(39523..41058)
                     /gene="murJ"
                     /locus_tag="ACHUNN_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0574840.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41168..42091)
                     /locus_tag="ACHUNN_000356"
     CDS             complement(41168..42091)
                     /locus_tag="ACHUNN_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0574841.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42093..42740)
                     /locus_tag="ACHUNN_000357"
     CDS             complement(42093..42740)
                     /locus_tag="ACHUNN_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR0574842.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42751..43335)
                     /gene="rimJ"
                     /locus_tag="ACHUNN_000358"
     CDS             complement(42751..43335)
                     /gene="rimJ"
                     /locus_tag="ACHUNN_000358"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0574843.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43571..44779
                     /gene="mdtH"
                     /locus_tag="ACHUNN_000359"
     CDS             43571..44779
                     /gene="mdtH"
                     /locus_tag="ACHUNN_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0574844.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44843..45490
                     /gene="grxB"
                     /locus_tag="ACHUNN_000360"
     CDS             44843..45490
                     /gene="grxB"
                     /locus_tag="ACHUNN_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0574845.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45624..46184
                     /locus_tag="ACHUNN_000361"
     CDS             45624..46184
                     /locus_tag="ACHUNN_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0574846.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46290..47336
                     /gene="pyrC"
                     /locus_tag="ACHUNN_000362"
     CDS             46290..47336
                     /gene="pyrC"
                     /locus_tag="ACHUNN_000362"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0574847.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47410..47655
                     /gene="dinI"
                     /locus_tag="ACHUNN_000363"
     CDS             47410..47655
                     /gene="dinI"
                     /locus_tag="ACHUNN_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0574848.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11571 a        12915 c        11860 g        11424 t
ORIGIN      
        1 gtgattattt ttataataac ctttcattca cagtcaccag gttcaacggc atcatggcaa
       61 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
      121 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
      181 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
      241 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
      301 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
      361 ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact
      421 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
      481 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
      541 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
      601 tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc
      661 gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga
      721 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt
      781 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
      841 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
      901 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
      961 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc
     1021 accaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg
     1081 attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
     1141 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg
     1201 ttcaaacacg ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc
     1261 gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc
     1321 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg
     1381 aaatacttaa ggattcggag aagtctgaag gaatgcctga tgcgacgctt aacgcgtctt
     1441 atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc
     1501 cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca
     1561 ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata
     1621 gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt
     1681 tacccatcag gctaccgaca gtggagaagt tcgacagcga taccagcgaa ccatgatctt
     1741 tatactgata atttttcagc ggtttaccgt tcatctgcgc cagaatattt ttcattgcgc
     1801 aggtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg
     1861 ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg
     1921 gttccaccac cagctggttg atacggttgg tttcaagacc accgatatct ttcaggaagt
     1981 ctggcgcttt gatcccggct gcccacacca tcagatcagc ctcaatatat tcaccatctt
     2041 tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc
     2101 caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa
     2161 tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac
     2221 cgtagctgtg cagttgcttg accgcattgt acaattcagc ggagagttct acacccgtcg
     2281 cgccgccgcc gacaatcgca atgttcactt taccattcgc gcccaggttg gcggagtatt
     2341 tcaggaacag attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga
     2401 aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca
     2461 ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc
     2521 gcagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct
     2581 ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat
     2641 caagcgagcc agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat
     2701 cgaccagcgt aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca
     2761 tttccagccc accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg
     2821 tgaccccctt aaatttatta accaattgtt aattaatggt tataaacata gctcttaatt
     2881 aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat
     2941 tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc
     3001 gggtgagatc aaaaaacaga gtttattgtt tattatttga gcaagtccgg aagggtgata
     3061 tagggtgacg cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc
     3121 aggcattgat gctcgactgt cggatgcggc gtaaacgcct tatccgactt acagaattcg
     3181 gagcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt
     3241 gaacgcctta atgcgctgca aatgcgggga gatattcttg aatttgtgcg tctgctcttc
     3301 gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagcgc
     3361 ttcatcatta cgcgacaaaa tcaccaggca acgatcgcga ttcttctcgc ggaagttggt
     3421 cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt
     3481 ctcataaggg aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat
     3541 ccgttccgcc cagtatccgc ccagaccaac gccgcaaatc agcggacgct cgtcgacgtt
     3601 cagttgcaac attttgtcca cttctttgag cagatgctgc atatcatgtt tcggatgccg
     3661 cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc
     3721 gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca
     3781 taagccaggg ttagtgacct gctttctctt cctgccagcg ttcatgtaac tgattcagac
     3841 gggtgctgat cgccttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt
     3901 tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga
     3961 cggccccttt acgattattg cagaccagga tcatatcgca acctgcgtcc agtgatgcct
     4021 gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga
     4081 aaattacacc gtcaaaacac agttcctgac gcaaaacggt tttcagccag taggaagaac
     4141 cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga
     4201 gtttattttc gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt
     4261 gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg
     4321 ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg
     4381 caattgccag ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga
     4441 tatgcccgac atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc
     4501 tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg
     4561 cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc
     4621 caccttcctg atctaccgcc accaccagat gattgcgcga tgctgcgcgg atctggcgca
     4681 ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca
     4741 ccagcggatg cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca
     4801 acattactgg acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca
     4861 tcggccagcc tgataaattg ttgatcgccg atttgtcgcc agcggtactc aaaccaccct
     4921 gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga
     4981 taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc
     5041 acccacaccg ccgccagttc cagcgcgata tcaccatctc cggcatactc ccagtcgatg
     5101 agttttaacc ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga
     5161 cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa
     5221 cccacagtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa gggcaacagc
     5281 gttattcgcc agccaaaacg tggttgttga tgaagataat acagcaagcc tgccagttcg
     5341 ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat agtcgactac catccagtca
     5401 cggagatata aatgcggctt cggtgcaatg cttgcgggta gttgtgataa agcccgatac
     5461 tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc
     5521 tggccctgat gttcaatgag aaaactaccg ccactaagcc cactattaaa cgtcgtgacg
     5581 ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg
     5641 cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat
     5701 ttgtagggtc ggagccttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc
     5761 gacattgcgg gcaatgccta tggctttact acgggtacct aaactgtcct gcggcgacaa
     5821 acctaactgt tgcttagcca tcgacagctg ctgggcggaa accagggtaa atttcccgtt
     5881 attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt
     5941 acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag
     6001 catcttactg accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc
     6061 aggcggtgcc gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac
     6121 cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac
     6181 ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc
     6241 ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat
     6301 aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat
     6361 gtgcgggaat gctaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt
     6421 cataccagta aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg
     6481 aggatgctga aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg
     6541 cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat
     6601 gtgaacgaca gcccaccagc agtaacaacg ccagagacac cagcccaaag catccttttc
     6661 tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg
     6721 catatggatg tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata
     6781 gccatcttcg gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc
     6841 cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc ggaatgagga tattcggaat
     6901 gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc
     6961 ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc
     7021 tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca
     7081 ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct
     7141 taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa
     7201 tgcgctgaaa agacattctc tgtttggttt acacgacaaa tatgaatgcg catatttctc
     7261 atttgcattt acaaacaaaa ttattcgcac actaaatata accgctcatc accctcctcc
     7321 ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac
     7381 agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg
     7441 gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag
     7501 ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg
     7561 ggtaccaaaa tgcagatgac tcaacttgat attcctcagt cggtcactat tgttagccag
     7621 cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg
     7681 atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgtgg attccagatc
     7741 gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac
     7801 gcactttctg atatggcact ctttgaacgc gtagaagtag tgcgtggcgc gacaggactc
     7861 atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt
     7921 cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg
     7981 gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc
     8041 taccagaata acgactcatg gctggaccgc tacaacagtg aaaagacctt cttctcgggc
     8101 attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc
     8161 attgatgtta atagcccaac ctggggcggt ttaccgcgct ggaatactga tggcagcagc
     8221 aacagttacg atcgtgcacg cagtaccgca cctgactggg cgtacaacga taaagagatc
     8281 aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc gacactgaat
     8341 gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac
     8401 aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt tgattatgtc
     8461 ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt gttcgctgac
     8521 ggtagttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa
     8581 caaaacaatc gttacttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc
     8641 tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct ggcacaggac
     8701 gataccacac atatgaaatc gttatatgct gctactcgag tcacccttgc cgatccgctg
     8761 catctgatcc tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg
     8821 gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg
     8881 acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa
     8941 tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat
     9001 agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc
     9061 accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca
     9121 gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca
     9181 tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa acgccgttaa tcctaatctg
     9241 ccacgcacca cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca
     9301 gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc
     9361 accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg
     9421 acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc
     9481 aacatggaag gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat
     9541 caattctgaa taacacctgt aaaaaaggca gccatctggc ggccttagtc tccccaacgt
     9601 cttacggatt agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta
     9661 gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc
     9721 ttcaggccag cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata
     9781 catgcgtcga ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc
     9841 atttcgctgg tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc
     9901 agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata
     9961 ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacgat aaagtcgatc
    10021 agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc
    10081 aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaaggagaac
    10141 tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg
    10201 cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc
    10261 agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc
    10321 atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc
    10381 tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggcaccag gcaacgttcg
    10441 atgaaaccgt aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag
    10501 gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg
    10561 gcagccaggc cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca
    10621 ggcagcttaa tacggtagaa acggttaaac atgtacgctg cgatcgcacc ggagataatc
    10681 cctccgagta cgccagtatc cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc
    10741 agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg
    10801 gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc
    10861 agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg
    10921 ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggcagtacg
    10981 gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac
    11041 ataattgaga gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg
    11101 ggagagcctc gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat
    11161 aattcagtgc cgatttgttt gacggctatc acatttcaac ttcagatcat tggagaagtt
    11221 gcacattttg acaaaacata tgtcacaata tttcaaacgt ggttattcac cgcccaattt
    11281 tcggcggtga actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa
    11341 cggatggatt gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt
    11401 acctgcgcca gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca
    11461 cgaaccatcg caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag
    11521 tcagtttcca ccagtaaccg atccaggggg acataacgcg cagcatctcg aagttgctcc
    11581 gcattacgga aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta
    11641 cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt
    11701 tcttcgcgca gaattgccag cgtatcggcg cgggcgtcac gggtatggac gataaccggc
    11761 ttgttcagtt cacgaccaat ctggatatga tggatgaagg actcttgctg acgaacttta
    11821 gtttccggtg tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct
    11881 gccgccagac ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg
    11941 ccacaagaaa atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc
    12001 ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga
    12061 acgtcatcca cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag
    12121 tcgactaaaa acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg
    12181 caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt
    12241 gacagacatt aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga
    12301 gggagaaaga tggtttgcca gttcgacgac aaggcccggc acatcaacat tggtcacctg
    12361 cgcagcacca tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg
    12421 cgccggagct tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac
    12481 gctatatgcc aatgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa
    12541 cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg
    12601 tgacattgtc acttcgcgtg aaagccaggt cacggcgtac tgttccggcg gcggcgcaag
    12661 gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc
    12721 caggaaaaac caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc
    12781 ggcgtcggtc agtaaggcag catcggttac ccagacaact ttcgcaccac ctaggcgtgc
    12841 gtgctcattc agcttttcgg tgacctcacg tactgcatca atgcccagcg tatttttccc
    12901 tttttcggga gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc
    12961 acgacagtga ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag
    13021 ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg
    13081 acctcttccg gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata
    13141 ccatctcatg cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc
    13201 acggcctcca gcggctgggt ggcatcaatg gtatgaatac ttttatcttg tgcagccagt
    13261 tccagatagc gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc
    13321 agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc
    13381 gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg
    13441 tcaataccac gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc
    13501 acccaggtgc cgttagccag agctggtttg atgaccgttt ctaccagttg aacgcgcgcg
    13561 gcataaaaca tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata
    13621 tccagcacca ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa
    13681 accatgtcgc ggatacccag ttgctcaagc gtctcaacca ccacattacg cgcggtagtt
    13741 ttgcctgcgc cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag
    13801 cactttcaga taatcctgca cagacttgtt atggctggca agattggtat taaacgtgtg
    13861 accaccttta ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc
    13921 cttcagcgaa tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata
    13981 ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc
    14041 tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt
    14101 gataaatacc gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat
    14161 tgatgccatc gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc
    14221 ccaggcgcta tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta acgcgacatc
    14281 ggtggtattg gcggtataca tccaggtgtc tggccagaac caaccttcaa tccactccgg
    14341 gttttccagt tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt
    14401 gatatacgga gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag
    14461 tcgcagcggg aactgtgctt ctttaccgct ttccagcaat ttcagcatct cgcgcacggt
    14521 catctgcggt gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg
    14581 cagcaaccat tgaaaaaccc gtgggcgatt gatgatctta tcggcataaa gctgttcacc
    14641 aagcgccaga cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa
    14701 tttgctgtcg gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac
    14761 caccagcaat aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa
    14821 agtggggcta aatattcata taacgttgct gacgaaaagg agacatcgcc acaggcacgt
    14881 acgggcatca ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctct
    14941 tccagagagg cttgcacttc gacaagctga taagaggatt gtgccagcaa acggatacag
    15001 aattgtcgca taatgccgtt aacacctgcc tgatccagac gcggcgtata aactacattg
    15061 cccttccgcc agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg
    15121 accagcgcct catcagcgtt tgtctgctca agatgagagc ggatcaatac ttgctcaagc
    15181 cgattaagat gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc
    15241 gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg caggataaac cgtaacggag
    15301 agaatccgcg ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc actaccgcga
    15361 ctgatcacga ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt
    15421 tcaagctgag gccagaagtc acagaaaatc atcaaccgct gacaagcatc ctgtagtcgc
    15481 tggatatgcg ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa
    15541 ccatcaccaa actgcgttgc ccgatcgctt actgccagcg attcctgctt atgaccgtta
    15601 attaagaaca taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg
    15661 aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtggg
    15721 aaatgacaac ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg
    15781 agttacacag cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca
    15841 gatcgcaacc ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca
    15901 gcgccaggat ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca
    15961 tagatttcgt ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg
    16021 cctgcgcttc agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa
    16081 tctgactcgc ttcaatgcct gcatcacgca gagcatttgc catcgccaga gctgcgcctg
    16141 cgccattttc tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga
    16201 gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca
    16261 taccggcacc atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg
    16321 cttgcgggtt atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacaccca
    16381 gcggcgtact ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga
    16441 taatacgcgc agcatggcca atgttgtgca cgccggaagt acaggcggtc gcgatagaga
    16501 tgcttgggcc acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg
    16561 ttgacggaac gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt
    16621 tttcttcgat cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg
    16681 cgttctcttc cgttatatca aggccagaat cctgcatggc ctgaacgcca gcgacaattc
    16741 catattgaat gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt
    16801 taaaatcctt tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga
    16861 ttaggctgat gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc
    16921 cgacaggaga caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc
    16981 tccagggagg gaaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg
    17041 accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa
    17101 cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag
    17161 ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat
    17221 tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt
    17281 cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg
    17341 tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta
    17401 cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat
    17461 aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca
    17521 tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga
    17581 atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca
    17641 ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg
    17701 gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga
    17761 agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac
    17821 caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta
    17881 ccgcttttga cagacggaaa acagatgaaa ggttggtttc gataatatcg ttccactctt
    17941 catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat
    18001 ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg
    18061 tcacattcaa catcagacct ttgccgttgg cacctaaata atcgctgatc gcctgagcgc
    18121 cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa
    18181 ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca
    18241 tgattttcct cttttaaagc tcaagcgccg ctgccatcgc tgaaggttcg ttcagcgccg
    18301 aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt aagcaccttg cccgggccga
    18361 cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa
    18421 ccgggttata caactgacgt accagtgcgt cacggatggc atcaccattg gtttcgcatt
    18481 tcacatcaac gttattcaca acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt
    18541 ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg
    18601 gtaacggcag agcacgtttt gcgcccgcag ctttacaggc agcgccagca cgctcaaccg
    18661 cttctttatg accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga
    18721 cctgacctgc tgcagcttct tcacacgctt tcgcaataga cgcatcatcc agaccgatga
    18781 ttgccgccat agcgcccgta ccttccggta cggcttcttg catgagcttg ccgcgcatct
    18841 caaccagacg caccgcatca gcgaaatcaa tcacaccagc gcaaaccagc gcggaatatt
    18901 cccccaggct gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc
    18961 ggtacagcgc aacagaggca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt
    19021 cttcagctgg cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt
    19081 cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg
    19141 tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct
    19201 agaaacgaac cagcgcggag ccccaggtga atccaccgcc aaaggcttca agcagaacca
    19261 actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg
    19321 ccgcagaggt attaccgtgg cgatccagcg tcaccacgac attgtccata gacataccga
    19381 gttttttcgc cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca
    19441 gttgagaacg gtcaagatta tttgccgcca gcgtctcatc aacgatgtgc gccagttccg
    19501 ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat
    19561 tcacacggtc agcattaggc agcgtcagca actcaccata gctaccgtcg gcatgcagat
    19621 gggtggagat gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc
    19681 caaaaataat aatagtccca cgatcggttg gatcgcaggt gcgcgccagt acatcggaac
    19741 cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg
    19801 cataggtgaa acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgccca
    19861 gcatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca
    19921 caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg
    19981 cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac
    20041 cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg
    20101 tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc
    20161 agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc
    20221 taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat
    20281 tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca
    20341 atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc
    20401 aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc
    20461 aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca
    20521 ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaatgtg
    20581 acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg
    20641 gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc
    20701 cgaatactgt cgagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga
    20761 ttgggaattt ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac
    20821 attgtactgt cacaatcgac gttggcccct aagtcaagga ccaccgtttt gcccttttgc
    20881 tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat
    20941 aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga
    21001 ccttctttca ccagctccag ggccacgcgc attgaactcc cacgactggc gcggatagct
    21061 tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa
    21121 cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa
    21181 agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa
    21241 gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg
    21301 ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc
    21361 agacgcgtga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt
    21421 ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca
    21481 gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg
    21541 ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact
    21601 tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcacttct tcaggcagtt
    21661 caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta
    21721 ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt
    21781 cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca
    21841 caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac
    21901 actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga
    21961 tagcgaacga catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc
    22021 gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa
    22081 ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta
    22141 acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac
    22201 attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg
    22261 aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc
    22321 taaacttatt ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat
    22381 ctcttttgaa tgtgcagcac cagaggtcga cgaaacccca cgcagcgacg aatcaccgcg
    22441 acagttggtg cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac gttatccgga
    22501 tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc
    22561 gttaacggaa gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt
    22621 ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc
    22681 ttttgacgtc catttccgtc acctgagcga ggcggagatt gataattacg tgcgtaagga
    22741 gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga
    22801 gcgtttagag ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca
    22861 gatgttacgt cgggaaggga aaaacccgct gatgggatag ttgtttgcgc tggatgcggt
    22921 tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc
    22981 aaccgcccat tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac
    23041 aacgcttcaa accttcatcc atcggcgctt cgatacgcat cacctcaccg gtccccggat
    23101 gggtaaactt caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag
    23161 tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg
    23221 catactgtgt atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca
    23281 gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct
    23341 gactgacacg cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa
    23401 cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc
    23461 ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc gctatcagca
    23521 acacacctga agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc
    23581 gcaacgcccg caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg
    23641 taccggaagg tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca
    23701 gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa
    23761 ccggtgggat acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt
    23821 tgttcacccg cacttcgcct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt
    23881 tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa
    23941 caatttttac ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat
    24001 tcgcgccact catttttcta tgcttatatt tagttttgca ccttattact tcactacgtg
    24061 atcactttat tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa
    24121 gcgggtgata aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg
    24181 ccgtttccgt gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg
    24241 accgatcatc cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct
    24301 gcgggttgca gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt
    24361 tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct
    24421 ttgagctgac gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca
    24481 cccggaaagg cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt
    24541 acgatgaaaa gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta
    24601 gatgggcagc gtctttatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca
    24661 aacatctaca aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat
    24721 tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct
    24781 gctaactaca gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa
    24841 gtcattgttc agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt
    24901 atcagtctgg cgggtagcta tctggttctg atgccaaata acccgcgcgc aggtggcatt
    24961 tctcgccgta tcgaaggcga tgaccgtacc gaattaaaag aagcactggc aagccttgaa
    25021 ctgccggaag gcatggggct tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg
    25081 ctgcaatggg atttaagctt ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa
    25141 agccgcccgg ctccgttcct gattcatcag gagagcaacg taatcgttcg cgcattccgc
    25201 gattacttac gtcaggacat cggcgaaatc cttatcgata acccgaaagt gctcgaactg
    25261 gcacgtcagc atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtat
    25321 accggcgaga tcccgctgtt cagccactac cagattgagt cgcagatcga gtccgccttc
    25381 cagcgtgaag tccgtctgcc gtccggtggt tcgattgtta tcgacagcac cgaagcgtta
    25441 acggccatcg acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaaccgcg
    25501 tttaacacca acctcgaagc tgccgatgag attgctcgtc agctgcgcct gcgtgacctc
    25561 ggcggcctga ttgttatcga cttcatcgac atgacgcctg tacgccacca gcgtgcggta
    25621 gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt
    25681 tctcgctttg gcctgctgga aatgtcccgc cagcgcctga gcccatcact gggtgaatcc
    25741 agccatcacg tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg
    25801 tcgctctcta ttctgcgttt gatcgaagaa gaagcgctga aagagaacac ccaggaagtt
    25861 cacgccattg ttcctgtgcc aatcgcttct tatctgctga atgaaaaacg ttctgcggtg
    25921 aatgcgattg aaacgcgtca ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg
    25981 gaaaccccgc actaccacgt gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc
    26041 tacatgctgc cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct
    26101 gaacgtaagc gtcctgaaca acctgcactg gcaacctttg ccatgccgga tgtgccgcca
    26161 gcgccaaccc cagctgaacc tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg
    26221 gcaacaccag cagctcctgc acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa
    26281 gcgctgttca gcggtggtga agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa
    26341 gcgaaaccgg aacgtcaaca ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt
    26401 aatgagcgcc gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac
    26461 cgtcgtaatc gtcgccaggc acagcagcag actgccgaga cgcgtgagag ccgtcagcag
    26521 gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc aagcgccgcg tcgtgaacgt
    26581 agccgccgcc gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt
    26641 gaagagcaat ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt
    26701 cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg
    26761 gtagtcgcac cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca
    26821 gctccacgca cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa
    26881 cagcaagaag agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc
    26941 cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt
    27001 tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct
    27061 ggcaaagtct ggatccgcta tccaattgta cgtccgcaag atgtacaagt tgaagagcag
    27121 cgcgaacagg aagaagtaca agtgcagccg atggtgactg gggtccctgt cgccgccgct
    27181 gtcgaacctg ttgttagcgc accggttgtt gaagaagtgg ctgaagttgt agaagccccc
    27241 gttcagattg ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc
    27301 gccgcagtaa ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccaggaa
    27361 gttgcagaac acgcagaacc ggtggttgaa ccgcaggaag agacggcaga tattgaagaa
    27421 gttgccgaaa ctgctgaggt tgtggttgct gaacctgaag ttgttgctca acctgccgcg
    27481 ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtag ctgcggttga acctgaggtc
    27541 accgttgagc ataaccacgc tacggtgccg atgacgcgcg ctccggcacc ggaatatgtt
    27601 ccggatgcac cgcgtcacag tgactggcag cgccctactt ttgccttcga aggtaaaggt
    27661 gccgcaggtg gtcatacggc aacacatcat gcctctgccg ctcctgcgcg tccgcaacct
    27721 gttgagtaat aattagctca agtaatcaag ccctggtaac tgccagggct tttttatttc
    27781 atctttgaat caacatatta aaattcatcg aatggcatcc ttgctaacca acaatgcaaa
    27841 ataggcaaaa aaatgcccgc cccggcgatc ccagagcagg cagacaaaac atacccagtt
    27901 tcatgatatg ttttaaagcg aaattatttg ttgagctgga agagcgacaa tccctgcata
    27961 tcggtaaatg ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata
    28021 gttgcattcc agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga
    28081 tcgctaccta atgaatccag cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga
    28141 acggtcagca cattgttcag cgagtttttc agtccgcggt tggttttatc taacgccgca
    28201 gcggcggttt ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg
    28261 ctatccagca tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg
    28321 ttactggtaa tactgtcgaa aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa
    28381 gcatcgacct gttgcttaat actttctgtt ccgccgacat aatcaccgtt agcctcacta
    28441 aacggcgcag tctctgtttt ataaccggca aaaatgtagc gcccattacc gtcagtggtg
    28501 ttcgccagat tcagcaactg gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg
    28561 tcatcgtcac tcaaggtgcc attgctggcg tagacaattt tttcctgagc attctggata
    28621 gcagtggtga cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta
    28681 cgcgccagcg tgtactggct gttttgcgcc tgtgcctggg agagaacaac cgcttgtgat
    28741 gcagcaatgg gatcatctga agggttaacg actcgcttac ccgtcgacat ctgttcgccg
    28801 tacttcatcc attctgcctg agaattggtg ataccacgca tgttttgctg gtacatcatc
    28861 tgggtactga aacgcatttt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg
    28921 cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat
    28981 ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg
    29041 tcaccacatt accttgcgtg gtgctactgg ttttcaacgt cgcggtttta ttaccgatat
    29101 cactcactaa cgaggcataa gcgtcgttaa aggatttcgc accgcccacc gttttactat
    29161 tgctttgcag atccagcagg gcctgaccgt tgcggttatc gctatcaccc gcatcttttt
    29221 cgctcgccat cgctattttc gcttcgtcgg tgattaatac atccatgttg acgatggcgt
    29281 cacttactgg tttcagcgtg aagctgtcgt taacggcagg cgttcctgta aacgtcaact
    29341 ccagaccatc aaatgccact ttaccgttgg catccggcgt caccgtaaaa gtggtattgc
    29401 tggcaaggcg ggtgacctgc cactgattat tatcgaacga gattttgtaa tctgtcgcca
    29461 gtaccacgga ggcatcagtt accgtggcac cgatcgcaac gtcacctttg tttttcgtgt
    29521 tttgcagaac cgcgggctta ccgatagtaa agaaatcttc accagcatcg ccgttagcat
    29581 caaatccggc tttgtgttgg gtgttgaaag cctcggcaaa tgccagcgcc agttgtccaa
    29641 gcgtattacg cgtctggtcc agctcctgag aacggaatgt cagaatgccg cccagcgacc
    29701 cggtattcag taatttctcc gggatctcaa tattgcctgc cgtcccatca acataagcga
    29761 cagtcgtacg agaagggtca gcgctggaag gaactgccgc cagttgccgc gccgtacttc
    29821 cctgaaccag tgagtaacca ttggccaccg tgatgttata agtgccgcca tcctgaacgc
    29881 tgacttctac accaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca
    29941 gattgttagg tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc
    30001 tggcaatttg tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga
    30061 cctgtttgtc ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt
    30121 ctgatttccc aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc accagcgttt
    30181 gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca
    30241 tattgtcgat tttcgacatc tgctcatagc gggcggtcag gccgctactt tgcgtctgcg
    30301 cagcacgtaa ctggttggta ataaacgcat catactcacg ctgcacacca gaaacgtaga
    30361 cgccattgcc aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg
    30421 tttggcgggt atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg
    30481 ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc
    30541 cttataagcc ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca
    30601 gaacagatta tcaatgttca tgctgtaggt tttgctcacc ttgtcgctta tcgatttcat
    30661 ctgctggatc atgttggtga gtttacgggc atagtgagga tcggtagcat accccgcgtc
    30721 ctgtagggcc tgcgccccct gctccgcact cgcggctgtc gtcacggcgg cgtagcgcgg
    30781 gttacgtgtt aacagcccaa cgtaatccga caatgcttcc agatacgagc tgtagacccg
    30841 aaatttcgct tttactttct tcgcttcgcc attttcatat tcagtcgtgg tgatttcagt
    30901 aactggacct ttccagttgc cagaggcttt gacgccaaac aggttatagc tcggctcgcc
    30961 gttttcgcgg cggatttgcc gttgccccca accagactcc agcgccgcct gagcgaggat
    31021 caaatgatgt ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc
    31081 gaggaatgct ttactgtcac ccggcagcga atcatcgtag ttacgtggca cggccttttg
    31141 caccagctgc gaaagcgcct gattttgata acgcaccaca gtttcgagcg ggaatttcat
    31201 cggtgctgct ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat
    31261 catctctgca agccccagac ctttgcccgc cgtcatctgt tgggcaatct gctggtcata
    31321 catactggta tacaggcgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc
    31381 gcgcatgctt ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg
    31441 gatatttgcc gccggatctt cgcccgcttt cgcctttagt tcgttgagtg attgcgcatc
    31501 ccaggccgca cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc
    31561 cgcagacatc ccgcactttg cattgattgc agtatggaca tcagatccat cggcgtagcg
    31621 cccagcgcat tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg tacgctttgc
    31681 agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg gagtaaccac agtctgtcca
    31741 ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac gattgactgt tactgagaga
    31801 tttccctgcg ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac caccgaaccg
    31861 gtgcgcgagt taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg
    31921 atatctgcaa ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc
    31981 cgcgcatcta acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt
    32041 tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca
    32101 aactggctgg gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt
    32161 tggttcacct gaacactgct accgccagcg gaggctcctg cgccgccaac cagaatattg
    32221 ccctgcgcca gcgcatacac ctgactgtca acgcccttaa gcggcgtcat caacaacgta
    32281 cctccacgca agcttttggc atttcccatg gaagaaacca ccacatcgat ggtttgcccc
    32341 tgacgtccaa acggaggaag tgactctgtc accattaccg cagcgacgtt ttttagctgc
    32401 atattggtgc ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt
    32461 gtggtaaacg gcgtctgggt tgtctggtca ccggtgccat ccagccccac caccagacca
    32521 tagccaatca gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca
    32581 gcctgagccg ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca
    32641 gcctcactta cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg
    32701 cttcgttaat gtagccattg cctacgtatt caatgcgcgc atccgccacc tgagtagacg
    32761 gtacggtatt gctgccgctg atagtgcgtg gattaaccac gccagagaag cgaataaatt
    32821 cggtaccctg attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca
    32881 gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc
    32941 cctttccgtt gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca
    33001 gcccctgcaa atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg
    33061 cattcgcaga ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat
    33121 cgccaatatt gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct
    33181 gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac
    33241 tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca
    33301 ccaacaagct ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca
    33361 aaaatcagta aaccggtgaa cctgccaccg gttaagcctt agagttgcgt cagtttttgc
    33421 agcatctgat cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga
    33481 atcatattga ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac
    33541 agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg gtacaccaga ggattgcgtt
    33601 tcggtgtaga ggttttcgcc aatgctctcc agcccggtgt cattcataaa ggtggtgaga
    33661 ttgagctgcc caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca
    33721 tcacgaccga tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga
    33781 aaaccaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg
    33841 aaccatctgg caacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt
    33901 tgttggtctg cgacaggttt ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc
    33961 ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct
    34021 ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag
    34081 tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc
    34141 ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaaa
    34201 gtgcatatgt cttcttgccg gatgcggcgt aaacgcctta tccggcctac aagtgctcgc
    34261 aaaatcgata aattgcgatt gcccccatag acccgataaa cttgcgcatc gggcaactta
    34321 tacttgtcat gtatcctttc aattaactca tcgacagcag ttggttggca cggcctgcgt
    34381 tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca
    34441 tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaaccccc gacatcacac
    34501 gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct gcgcttaaac
    34561 gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc agtttcagac
    34621 gccctactgg cgcaactgta tttgccggat cgcccggatt gagcgccgag attgtgccat
    34681 cggcagcaat agtgatttcc gctccttccg gcacagcaat tggccccgcc tcgcctatca
    34741 cctgatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg
    34801 tatacccttc gctgccgtca gcggtctgca cggccagcca gccatcctgc tgcaacgcga
    34861 cgtccagcgg gcgcgaggtg taatccattt tgccgggcgt catatctgcg cccggcgttg
    34921 acgccgtgac caacgtgcgc gtgggcagag aaagcccttc cactggcacc gcgcgtaaag
    34981 cattcaactg cgcgcgaaaa ccgggcgttg aggcattggc cagattactg gcggttaccg
    35041 cctgttgatt cagtgtctgg ctggctgctc ccatcgcggt ataaattgcg tgatccattg
    35101 agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg gtcctgagtt
    35161 ttgatggtct gggcgttaga ctgatagtta cgctgggcaa cgatcatatt gaccagttct
    35221 ttactgagat cgacattgga cgcttccaac gcaccgttgg tcagggtgcc aaagttgccc
    35281 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    35341 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    35401 tgtgtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat ttgataactc
    35461 accagatcgc ccggtttgta gccattctgg gtggttgcca caatattgtt agcgccggta
    35521 ttttgctgca tggagttgag gaagctcagg ctaaacgtgg cgggatctgc gccgttaatt
    35581 gcgccagtgg taatattctc tgttggattt gaggtcagaa cgccattggc attaaacacc
    35641 agcttcattg caggctcggc tgtacctgtt ggatcactgc tatcctgggt gtagacctgc
    35701 cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt accctgactg
    35761 tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc gctgaatggc
    35821 gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc cgtggtggta
    35881 gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc cccttgctga
    35941 atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg catattcacc
    36001 aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac cgaaccgttg
    36061 ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc tcgcccggtg
    36121 ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac ccccagtccc
    36181 actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa gccgtaggtg
    36241 gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc gtttaatccg
    36301 ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta aattatctgc
    36361 cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt accgctgttg
    36421 ccgcggagca caccctgcac cagagcaaac tgcagcggct gggcaaccag ttgtgtacca
    36481 ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct gccatcagtc
    36541 aacgtaccgt cccaggtgaa actgtggact ccggcggtca gttcaccaat atcaatggtg
    36601 cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc cgcctgttgc
    36661 agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc ggttcccgca
    36721 agaacagtgg tgccggggat catcacgccg tgaccgatca ggttactggc ctgtaacgac
    36781 tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt ttcaatcccg
    36841 ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat tggattggtc
    36901 gggtcctggt ttttcagctg cgccaccaac aaagtcagaa aactgctttg taagtctgcg
    36961 gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt tgtcggatcg
    37021 gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta agggttttca
    37081 gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg cgtgacgccg
    37141 acatggtgtt aaccatctcc ccgacgacat caacgttcgg catttttacg tagccctttg
    37201 catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga ctttctataa
    37261 catcggcaac ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca
    37321 cctgttttgc ccgatatggc tgtccatcgg gaccggtcac gctatcagca ttcgccagat
    37381 tactggccgc cacgttcaag cgctgggact gggcagttaa cgccgacccg gcgatatcaa
    37441 aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga
    37501 tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg
    37561 tgcgttcgcg atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt
    37621 attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc gttgaggtca
    37681 tcgtcagtgc aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac
    37741 tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg
    37801 ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    37861 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat
    37921 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    37981 atgaccattg cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctgg
    38041 caataaaacg tagcgtggcg atcatctcga tactattcac tccgttaagt gcggcgagta
    38101 atctcacatc gcaattgcaa acctttttta gcgcccagct cgcgggggta agtgatgagg
    38161 ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc gtgcgagcag ccattgcttt
    38221 cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg
    38281 acaaacgata tttacaggtt aatgtacagg ccacaggaaa ttatgtggtt gccgcgatgc
    38341 ccattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actaaaacgc ggacggcagg
    38401 ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc
    38461 gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa
    38521 aagcgggaca acgcgtcaac gtgatcgcca gcggtgacgg gtttagcgcc aacgcagaag
    38581 gtcaggcgct gaacaatgca gccgtcacac agaatgcgcg ggtgcgcatg gtatcgggac
    38641 tggtggtcag cggcgttgtt gatgcagatg ggaatattct tataaaccag taagctgtta
    38701 aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg
    38761 aataaaccat gagtattgat cgcacttcgc ctctgaagcc cgtaagcacc gttcaaccac
    38821 gcgaaaccac tgacgcgtcg gtaacgaaca cccgggcggc aaaaacaaca gcctccacca
    38881 gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata
    38941 tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg
    39001 acaccggcaa aattgccgat gcgctgctca acgaagcgca gcaagacttg cagagtaact
    39061 gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc
    39121 aaaacggtaa tggatcaaga acagcaacat ctctctatgg ggcagatcaa cggcagccag
    39181 ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag
    39241 ttacgcagga aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa
    39301 attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa
    39361 ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg
    39421 ctatatgggg cgaacggtca gacctcaaca acccatcgcg gcggtaaaaa gatttcgatc
    39481 tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc
    39541 aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc
    39601 cagcacgacc gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg
    39661 catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg
    39721 caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca
    39781 gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccagcatg
    39841 tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc
    39901 aattttcact ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac
    39961 tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc
    40021 atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac ccgaaagaat
    40081 gcccaacgca accgcactcg gcagcgccaa caggaaacaa agacgcaacc cccagtccat
    40141 caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa
    40201 aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta
    40261 atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa
    40321 ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat
    40381 tgctccggca tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg
    40441 cggtagctga tacaccagct gcaggatgcc gccgaccgta acagcccacg ccagcgccag
    40501 caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag
    40561 cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac
    40621 cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta gctggctggt
    40681 cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg
    40741 tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac
    40801 ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat
    40861 cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag
    40921 tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat
    40981 tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag
    41041 cgattttaat aaattcatcg gtgttctaat ccagacccac gggcaaaacg cctgcaaggc
    41101 aggcgctgaa agaggcgaat agtctacctg gattatggtg aattgctacc gccagatgtt
    41161 acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca
    41221 ctgcctgttc gccggcggtt tgcagaactg tctgattttg cacacattca atgaagtgcc
    41281 gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat
    41341 gcactacgcc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg
    41401 catcgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacagg
    41461 tggtgatctg caaaggatcg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt
    41521 cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg
    41581 tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacac
    41641 tattgctacg atgtttatcc attcttagcg aagctgcggt agcgagttgc gtttttaact
    41701 caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg
    41761 ccgccagttc caccagccgt tcagcatcgc gcagattttc tgccagcggt ttatcgacac
    41821 agacatgtac acccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg
    41881 aatgcacaaa aaccgcatcg caactggcgg caaggctgga taacgaatcg gcataaggaa
    41941 tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt aggcgaccag gctccttgta
    42001 acatccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta
    42061 atcccacaac gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa
    42121 tcaagacgct gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggcctgtaaa
    42181 tcaccatcaa ccgcgttcga catatccgtt accgccggct gatcctctac ctcaccactg
    42241 aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa
    42301 ggaccatctt cgcgatttgc cagttgttcc agcgtcgact ccacttccgc catatcgctg
    42361 aattcataca ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat
    42421 aacaacgtgg tgattaacgc cacttccgct gcgctcagtt tcagatcgcc aaattctgaa
    42481 ttacaaaaac gttgttcata tttggtgacc cgattaccaa agccactcac cgtgcgcaga
    42541 taatggcgtt tgaccagatt atccagttgc tcctgcactt cagattcact cagattcatg
    42601 accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg
    42661 tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caatcacgcg agcttcaagg
    42721 gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt
    42781 taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata
    42841 gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat
    42901 ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt
    42961 cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata
    43021 gcaggcatga aaagagccac gaacaacatt ggaaaaattg gcaacgccaa taatctcttt
    43081 ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat
    43141 catccccagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc
    43201 ccagggcttg aggaaatggc gattctctgc gtaataatcc gcaagacgcc aggcatcacg
    43261 atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact
    43321 gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta
    43381 ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt
    43441 cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat attgtccccg
    43501 aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc
    43561 gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat
    43621 cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt
    43681 cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt
    43741 tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc
    43801 gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca
    43861 cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga
    43921 tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtt gtcgtttttt
    43981 ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt ggcgcattgt tggggagctg
    44041 gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctgtg
    44101 tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt
    44161 tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct
    44221 ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga
    44281 cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt
    44341 aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt
    44401 gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggtct
    44461 gcaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc
    44521 gcgtgaaacc ttaagtgctt tgctggcgga cgcaagagct cgcggcagct atatggggtt
    44581 tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt
    44641 tgacctgggc aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg
    44701 catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgcaa cgcgtcgttt
    44761 gctggaacgc gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact
    44821 gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct
    44881 caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa
    44941 cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa
    45001 agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga
    45061 cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt
    45121 caatggctac gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt
    45181 ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt
    45241 tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc
    45301 gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat
    45361 tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg
    45421 cgttgctgat taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat
    45481 ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt
    45541 tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg
    45601 cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca
    45661 gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta
    45721 accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg
    45781 acgcccatat tgttctgaca aacttgatca gccaaattgg tcgcgaagag ccgaataagg
    45841 ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga
    45901 ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc
    45961 tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc
    46021 aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt
    46081 acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca
    46141 ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc
    46201 gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa
    46261 acattccttc agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc
    46321 gccgcccaga cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc
    46381 cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga
    46441 ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc
    46501 acgatttcac cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg
    46561 agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca
    46621 ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg
    46681 aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt
    46741 ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg
    46801 cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg
    46861 gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata
    46921 tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc
    46981 accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg
    47041 attctgcgcc acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca
    47101 acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc
    47161 actttgaagc attctgttct gtaaacggcc cgcagttcta tgggttgccg gtcaacgaca
    47221 cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg
    47281 acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat
    47341 cgtagcttcc tgttgtcatt aggttattta acctgtataa ataaccagta tattcaacag
    47401 ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct
    47461 attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa
    47521 ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa
    47581 gaagataaac agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg
    47641 tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg gcttttttat
    47701 tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg
    47761 ctaaaaaaat
//
LOCUS       DAZOXR010000007        44444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZOXR010000007 DAZOXR010000000
VERSION     DAZOXR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.007"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHUNN_000364"
     CDS             complement(<1..716)
                     /locus_tag="ACHUNN_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0574849.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUNN_000365"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUNN_000365"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0574850.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUNN_000366"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUNN_000366"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0574851.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHUNN_000367"
     CDS             complement(3650..4705)
                     /locus_tag="ACHUNN_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0574852.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHUNN_000368"
     CDS             complement(4715..5503)
                     /locus_tag="ACHUNN_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0574853.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHUNN_000369"
     CDS             complement(5521..6573)
                     /locus_tag="ACHUNN_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0574854.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="ACHUNN_000370"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="ACHUNN_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="ACHUNN_000371"
     CDS             complement(7194..8075)
                     /locus_tag="ACHUNN_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0574855.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="ACHUNN_000372"
     CDS             complement(8096..9406)
                     /locus_tag="ACHUNN_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0574856.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIVLPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..10951)
                     /locus_tag="ACHUNN_000373"
                     /pseudo
     CDS             complement(9441..10951)
                     /locus_tag="ACHUNN_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHUNN_000374"
     CDS             complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHUNN_000374"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0574857.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHUNN_000375"
     CDS             complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHUNN_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0574858.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHUNN_000376"
     CDS             complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHUNN_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0574859.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHUNN_000377"
     CDS             complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHUNN_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0574860.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHUNN_000378"
     CDS             complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHUNN_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0574861.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13254..14231
                     /gene="pspF"
                     /locus_tag="ACHUNN_000379"
     CDS             13254..14231
                     /gene="pspF"
                     /locus_tag="ACHUNN_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0574862.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHUNN_000380"
                     /pseudo
     CDS             complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHUNN_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHUNN_000381"
     CDS             complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHUNN_000381"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0574863.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHUNN_000382"
                     /pseudo
     CDS             complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHUNN_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHUNN_000383"
     CDS             complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHUNN_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0574864.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHUNN_000384"
     CDS             complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHUNN_000384"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0574865.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20134..21495
                     /gene="puuA"
                     /locus_tag="ACHUNN_000385"
     CDS             20134..21495
                     /gene="puuA"
                     /locus_tag="ACHUNN_000385"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0574866.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21644..21808
                     /locus_tag="ACHUNN_000386"
     CDS             21644..21808
                     /locus_tag="ACHUNN_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0574867.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21798..23183
                     /gene="puuP"
                     /locus_tag="ACHUNN_000387"
     CDS             21798..23183
                     /gene="puuP"
                     /locus_tag="ACHUNN_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0574868.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23317..23562
                     /locus_tag="ACHUNN_000388"
     CDS             23317..23562
                     /locus_tag="ACHUNN_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0574869.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23875..25518
                     /gene="sapA"
                     /locus_tag="ACHUNN_000389"
     CDS             23875..25518
                     /gene="sapA"
                     /locus_tag="ACHUNN_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0574870.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25515..26480
                     /gene="sapB"
                     /locus_tag="ACHUNN_000390"
     CDS             25515..26480
                     /gene="sapB"
                     /locus_tag="ACHUNN_000390"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0574871.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26467..27357
                     /gene="sapC"
                     /locus_tag="ACHUNN_000391"
     CDS             26467..27357
                     /gene="sapC"
                     /locus_tag="ACHUNN_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0574872.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27357..28349
                     /gene="sapD"
                     /locus_tag="ACHUNN_000392"
     CDS             27357..28349
                     /gene="sapD"
                     /locus_tag="ACHUNN_000392"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0574873.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28351..29157
                     /gene="sapF"
                     /locus_tag="ACHUNN_000393"
     CDS             28351..29157
                     /gene="sapF"
                     /locus_tag="ACHUNN_000393"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0574874.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29225..29578
                     /locus_tag="ACHUNN_000394"
     CDS             29225..29578
                     /locus_tag="ACHUNN_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0574875.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29946..30734
                     /gene="fabI"
                     /locus_tag="ACHUNN_000395"
     CDS             29946..30734
                     /gene="fabI"
                     /locus_tag="ACHUNN_000395"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0574876.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30879..32005
                     /locus_tag="ACHUNN_000396"
                     /pseudo
     CDS             30879..32005
                     /locus_tag="ACHUNN_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32073..34007
                     /gene="rnb"
                     /locus_tag="ACHUNN_000397"
     CDS             32073..34007
                     /gene="rnb"
                     /locus_tag="ACHUNN_000397"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0574877.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34085..34188)
                     /locus_tag="ACHUNN_000398"
                     /pseudo
     CDS             complement(34085..34188)
                     /locus_tag="ACHUNN_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHUNN_000399"
                     /pseudo
     CDS             34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHUNN_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHUNN_000400"
     CDS             complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHUNN_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0574878.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHUNN_000401"
     CDS             36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHUNN_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0574879.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36642..37391
                     /locus_tag="ACHUNN_000402"
     CDS             36642..37391
                     /locus_tag="ACHUNN_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0574880.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37660..37878
                     /gene="osmB"
                     /locus_tag="ACHUNN_000403"
     CDS             37660..37878
                     /gene="osmB"
                     /locus_tag="ACHUNN_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0574881.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="ACHUNN_000404"
     CDS             complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="ACHUNN_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0574882.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="ACHUNN_000405"
     CDS             complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="ACHUNN_000405"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0574883.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="ACHUNN_000406"
     CDS             complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="ACHUNN_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0574884.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="ACHUNN_000407"
     CDS             complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="ACHUNN_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0574885.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="ACHUNN_000408"
     CDS             complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="ACHUNN_000408"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0574886.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41827..42417
                     /gene="ribA"
                     /locus_tag="ACHUNN_000409"
     CDS             41827..42417
                     /gene="ribA"
                     /locus_tag="ACHUNN_000409"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0574887.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42481..>44444)
                     /gene="acnA"
                     /locus_tag="ACHUNN_000410"
     CDS             complement(42481..>44444)
                     /gene="acnA"
                     /locus_tag="ACHUNN_000410"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0574888.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVE
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVI
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10459 a        11766 c        11218 g        11001 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc acaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg
     9541 acattaaaca atccagtcag acaatcacca tcagcgttac ttctttgaat acgcattacg
     9601 gatgaattaa tcgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9661 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9721 ttcagttctc gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat
     9781 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9841 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt
     9901 tcttcatcgc tactctcacg gcggcttaac gcatccatat gtgagaggcg aggaagttaa
     9961 accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa
    10021 cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat
    10081 gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct
    10141 cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa
    10201 tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa
    10261 cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca
    10321 tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg
    10381 tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac
    10441 gcgggcgagt gacggcgctg agatcggttt gcggatcaac cgcaataaaa aaatcttcaa
    10501 aacctggctg ctgttgtaaa tagtttttaa accattcact ttttgccgac atatggttgc
    10561 agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc
    10621 ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa cccatcatca gatgaacatg
    10681 gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact
    10741 gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta
    10801 acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac
    10861 tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt
    10921 cgtccaggta atccgtaatt ttctgtttca tgttgccttt ttgtggtagc ggtcccatga
    10981 aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    11041 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg
    11101 ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc
    11161 ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg
    11221 gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa
    11281 tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc
    11341 ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga
    11401 acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt
    11461 aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc
    11521 ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat
    11581 tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc
    11641 gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa
    11701 gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc
    11761 agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc
    11821 ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc
    11881 gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccag
    11941 cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc
    12001 gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt
    12061 ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt
    12121 attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata
    12181 gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat
    12241 cgctgctgct cactttgcga caattcatcg cgaccagaac gattgctgta atgcagccat
    12301 aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg
    12361 ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt
    12421 attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat
    12481 cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt
    12541 ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga ttcgaaacga gccattgctt
    12601 catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat
    12661 gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc
    12721 caatctcttt cttcatgcgt gccagcgtat cgttcaccag cgtcacttca tgttccaggg
    12781 acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc
    12841 tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt
    12901 gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta
    12961 cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt
    13021 ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa
    13081 tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata
    13141 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
    13201 gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg
    13261 aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt
    13321 cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag
    13381 agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc
    13441 ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg
    13501 gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc gacggcggta
    13561 cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg
    13621 tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc
    13681 ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg
    13741 ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg
    13801 aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc
    13861 tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc
    13921 cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc agtgtatcgc cacggcacca
    13981 gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg
    14041 ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc gctggattta cgtgagtttc
    14101 aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca aaaggggaaa tataaccaga
    14161 agcgcgcagc tgaattgctg gggttaacct accatcagtt ccgcgcgttg ttgaaaaaac
    14221 accagattta gcgaagttgg gcaatgcgat tcgccggatg cggcgtgaac gctttatccg
    14281 gcatacataa tcaacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
    14341 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
    14401 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
    14461 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    14521 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
    14581 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
    14641 tgattcgcgc gttcgcagag tgattctttg ttgataatat tgagcaccgc gtgcgcggca
    14701 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
    14761 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    14821 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
    14881 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
    14941 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
    15001 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
    15061 gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
    15121 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
    15181 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc agggcgtccg
    15241 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaac
    15301 gcggttttgg cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg
    15361 tagctttcat acggcacaat ctgatacgcg gtgtgggtaa actgttgcag ttgctgctcc
    15421 accgccgcga ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg
    15481 aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag cgttttcagc cgactgggcg
    15541 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    15601 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    15661 cagacgatcg cgcaggctgt accaggctgc gcccatcgcg gtaaacggta ctcgcagcgt
    15721 gcgcccgccg gggaacgggt aatacggcag attggcgaag gcatcgaaac gttcggcgtc
    15781 gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc
    15841 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccaaactgcg gcatgcgcga
    15901 cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
    15961 gaaggttttc agcagtttcg gcacaacaag acgctcaacg tcatccgggt cgcgcgcgcc
    16021 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
    16081 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    16141 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    16201 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    16261 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
    16321 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    16381 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
    16441 gacctcgcgg cgaatggcgt tagcgtccag cagctccagc tgtttattgc cgtagcgttc
    16501 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
    16561 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
    16621 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    16681 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
    16741 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
    16801 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
    16861 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
    16921 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
    16981 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
    17041 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcggcggc
    17101 tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
    17161 tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg
    17221 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc aacatccaca aagatggtcg
    17281 ggccgatggc ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt
    17341 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcaatcgatt aaggtgccca
    17401 tggtggttac ggtatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    17461 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
    17521 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
    17581 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    17641 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
    17701 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc ggcttcatga ccataaccgc
    17761 tcactacgtt cagcacgcca tccggcagcc ccgcttcttc ttttgccagc ccggcgagac
    17821 gaatcgcact gagtggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    17881 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    17941 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    18001 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    18061 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    18121 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
    18181 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    18241 catggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgaccg
    18301 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    18361 agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    18421 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
    18481 gagatccccg ggccgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    18541 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    18601 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    18661 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    18721 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    18781 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    18841 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac
    18901 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    18961 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    19021 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    19081 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    19141 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    19201 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    19261 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    19321 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga
    19381 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    19441 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    19501 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    19561 accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    19621 ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    19681 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    19741 ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc
    19801 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    19861 cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc
    19921 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta
    19981 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt
    20041 tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat
    20101 aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag
    20161 aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc
    20221 tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt
    20281 tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat
    20341 ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg
    20401 ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct
    20461 tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc
    20521 gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag
    20581 cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca
    20641 caaagccagg tttactccgt tgataacctc aaccacttcg ccgacgtgct caatgatatt
    20701 gatgaactgg cgcagttgca gctgattccg gcagatggcg cggtcgctga ggcctcgccg
    20761 ggtcagtttg aaatcaacct ttaccatact gataacgtgc tggaagcctg cgatgatgcg
    20821 ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc ataagatgca cgccactttt
    20881 atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc atatccatat cagtatgcaa
    20941 aataatcgtg gcgagaatgt gctttctgac acagaaggcg aagattcgcc gctgctgaaa
    21001 aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac
    21061 gtgaactcgt atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc
    21121 cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg
    21181 gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc
    21241 ggtattttgc atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg
    21301 ctggaacagg aaggcttacc cttcccgatt cgccagagcg atgccctggg tgagtttatc
    21361 gagaatgatc acctgcgccg ctatttaggc aaacggtttt gccatgtgta tcacgcctgt
    21421 aaaaatgatg agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg
    21481 ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg gcctgagcgg agccaggcct
    21541 gacatttgct ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac
    21601 gggcttccgt taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc
    21661 cccgcatggc gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc
    21721 tttgcttccc ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg
    21781 caggggaggc agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca
    21841 aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc
    21901 tcacgccgat gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg
    21961 ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca
    22021 aactggttcg ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta
    22081 acccgcacgt cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa
    22141 tgatcaacgt tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt
    22201 gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc
    22261 tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta
    22321 tcttcctggt ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc
    22381 agccgtttat cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct
    22441 gtttctcgtt cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg
    22501 cacgcgtgat cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg
    22561 cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg
    22621 ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct ctttcagtcg attttcctct
    22681 gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc
    22741 tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc
    22801 caaaatggcg gactccggca ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc
    22861 tgttctttga tttagtcacc gcgacagcac tgattaactt cggtgctctg gtggcgttta
    22921 ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg taaaggaatg aataaaagct
    22981 ggaaggatca cttccactat ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc
    23041 tgtgggttaa cctagagtca acgtcactga cactcggtct ggtatgggct tcgttaggcg
    23101 gcgcatattt gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca
    23161 gaacgccggt gagcgaaacg taatagccgg atgcgcggcg cattatcctg cctgagggta
    23221 acgcgccgcg ccattaacta taaaaagatg tataacccgg atcacgagtg ctatcatccg
    23281 cccttatgct tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa
    23341 tattttgata ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa
    23401 cgcacaccgc tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag
    23461 atcagcgaag aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt
    23521 gatgagatcg cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga
    23581 tcggtcagta tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc
    23641 actgcagcgt gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt
    23701 tctgtgagtt atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca
    23761 cctgttcaac gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc
    23821 aaatgtcatt aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc
    23881 caggtattat cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg
    23941 cctgaatccc ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg
    24001 caagtcaaca cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc
    24061 gcccagtttt atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa
    24121 cttgccgaaa gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc
    24181 gatgttccgt ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat
    24241 gtggtgttta ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc
    24301 agcaacttcc cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa
    24361 ctggataatc ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac
    24421 ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa
    24481 gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa
    24541 taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg
    24601 ttaatgccgc aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc
    24661 ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt
    24721 gacgacccgc gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt
    24781 aacaccgcca aaccgccgct aaataatccc gctgtccgcc atgcactggc actggcgatt
    24841 aataaccagc gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt
    24901 ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg
    24961 aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact taacgctgaa actgtgggtg
    25021 cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg
    25081 gatatggcgc aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag
    25141 gcgcggttga tggatatgag ccatgatctg acattatccg gttgggcgac ggacagtaac
    25201 gacccggaca gtttcttccg tcctttactg agctgcgcgg caattcattc acagaccaac
    25261 ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag
    25321 cagctggcgg cgcgtattga agcctatgac gaagcgcaga gtattctggc gcaggaatta
    25381 cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt
    25441 ctggtactta gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat
    25501 gaggtgaaaa aaccatgatt atcttcacct tacgccgcat tttgttattg attgtcacct
    25561 tgttcctgct gaccttcgtt ggcttcagct tgagctattt cacccctcat gcgccgttac
    25621 aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa cggcctgatc cactgggatt
    25681 ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg
    25741 cgacgatgga actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg
    25801 ggatgattgc cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct
    25861 tactgggctt ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac
    25921 tcacgctagg ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga
    25981 ttaccggttt tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga
    26041 tcatgagcgc aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa
    26101 ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa
    26161 aagcggcggc gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata
    26221 acgcgttacc gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg
    26281 cgatgatcac cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca
    26341 tccgccagca ggattacgca gccatttccg ccggagtgat ggtgtgtggc tcactggtca
    26401 ttattgttaa cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg
    26461 aatggtatgc cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc
    26521 gcctggcgca aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg
    26581 ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt
    26641 ctcggttatc aattattgcc gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg
    26701 gggactgacg acctcggacg cgatgtttta agccgtttac tgagcggagc tgcgccaacc
    26761 gtgggtggcg catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc
    26821 ttcgccgggg cgacacacgg cctccgctca gcggtgctta accatattct ggatacctta
    26881 ctggcgatcc cttcgctgct gttggcaatt atcgttgtag cgtttgccgg accgagtttg
    26941 tcccacgcta tgtttgctgt ctggctggca ttgctgccgc gtatggtacg ttcgatttac
    27001 agcatggtgc atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca
    27061 tcaacgctga atattctctg gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc
    27121 gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat
    27181 ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg
    27241 atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg
    27301 ttggttaacc tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc
    27361 cattactcga tattcgtaac ctgaccattg aatttaaaac cggtgatgag tgggttaaag
    27421 ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat
    27481 ccggttccgg caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc
    27541 gtgttactgc tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg
    27601 aacggcgcaa actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc
    27661 ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata
    27721 aaggccgttg gtggcagcgt tttggctggc gcaaacgccg tgccattgaa ctgctgcacc
    27781 gcgtggggat taaagatcac aaagatgcga tgcgcagttt tccctatgag ttgaccgaag
    27841 gtgaatgtca gaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctgattg
    27901 ctgacgaacc gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgctga
    27961 cgcgtctcaa ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc
    28021 ttagccaatg ggcggataaa attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc
    28081 cgagtaagga gttggtgacg atgccacatc atccttatac ccaggcgctg atccgcgcga
    28141 taccagactt cggcagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta
    28201 tcccactgct ggaacagtta ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac
    28261 aacgagaatg cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc
    28321 atttcccgct gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat
    28381 ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc gtcagaccgt agaagcggta
    28441 aaacccttga gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt
    28501 tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga tagagcctac cagcggcgag
    28561 ttattgattg acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt
    28621 cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt
    28681 ctggattttc cactgcgcct gaacaccgac ctggaaccgg aacagcgtcg caaacagatt
    28741 attgaaacga tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg
    28801 ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg cgctaatatt gcgcccaaaa
    28861 gtcattattg ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt
    28921 aatctgatgc tggagttaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat
    28981 atcggaatga tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc
    29041 gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg
    29101 attgccgggc actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt
    29161 tctgaatcct ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa
    29221 tgaggtgatg gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca
    29281 ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg
    29341 tcaacatccg gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt
    29401 tgagctggat ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg
    29461 gcttgagtcg cagggctgga ccgtgctgcg tttctggaat aacgagattg attgtaatga
    29521 ggaggcggtg ccggagatta ttctgcagga actgaaccgc cgatcaccct caccctgaaa
    29581 gggcgagggg gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg
    29641 agaatattcg gcaaggtctg aaccatccca gccatcgcca taaaagggtt aggggctgta
    29701 tgagcctgtt tgttgctggg gtaacaatat ttgcacaata tggtcccctc gcccctctgg
    29761 ggagagggtt agggtaaggg gaaaagcgcc caccctgccg cagcctgctc cggtcggacc
    29821 tggcaactat agctactcac agccaggttg attataataa ccgtttatct gttcgtactg
    29881 tttactaaaa cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
    29941 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactat
    30001 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
    30061 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
    30121 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
    30181 ggaaagtttg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
    30241 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
    30301 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
    30361 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
    30421 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
    30481 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg
    30541 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
    30601 ccgttactat tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg
    30661 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
    30721 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
    30781 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    30841 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    30901 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
    30961 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    31021 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
    31081 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
    31141 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    31201 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
    31261 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    31321 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    31381 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    31441 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    31501 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    31561 gttcaacagc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    31621 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    31681 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    31741 catcaaatga atataaaaaa tcagccccct tctgcgcaag ggcgaaaatg aaattaacca
    31801 atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat ggctgacccg
    31861 agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcggacacg aaaaatcatc
    31921 aacgcagatt attaatctgc tggtatggag cgggctgtgt ggctggataa atcgcttaaa
    31981 aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg taaaactgtc
    32041 agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg ctgctagcgc
    32101 agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta aaagccacag
    32161 aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag ttatttcatt ccgccgccgc
    32221 agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac
    32281 gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc gtgggtaaag
    32341 ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta aaagacgcca
    32401 ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac tgggcggttg
    32461 ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat
    32521 acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg cgccataatc
    32581 tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg
    32641 aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa gatatggatg
    32701 acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg gcgattgccg
    32761 atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa attcgcgcat
    32821 tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc tcgcgagctt tctgacgatc
    32881 tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg acgctctccg
    32941 ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa tccaaagcga
    33001 agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg cagcctgaaa
    33061 gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt
    33121 ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt attctcggtg
    33181 aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg
    33241 aagaagcgat gattgccgct aacatttgtg cggcccgcgt tctgcgcgat aagctcggtt
    33301 ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg ctggcagcgt
    33361 tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg gacggtttct
    33421 gcaaactgcg tcgtgaactg gatgcgcaac caactggttt cctcgacagc cgcattcgtc
    33481 gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc ctcggtctgg
    33541 aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc
    33601 tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc
    33661 aaatggccga acgtcgccgt ctcaaccgga tggcagaacg tgatgttggc gactggttat
    33721 acgcacgctt cctgaaagat aaagccggga ccggcacccg tttcgcggcg gaaattgtcg
    33781 atatcagccg tggcggcatg cgtgtgcgtt tggttgataa cggcgctatc gcctttattc
    33841 cggcaccttt cttacacgct gtgcgcgatg aactggtttg cagccaggaa aacggcactg
    33901 tacaaattaa aggtgaaacg gtttacaaag taactgacgt tattgacgtc accattgccg
    33961 aagtccgcat ggaaacccgc ggcattattg cgcgcccggt cgcgtaatct cctttcacgg
    34021 cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata tccaggcaga
    34081 ttttttaact gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat
    34141 atataaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt
    34201 atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc gttagggagt
    34261 ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca
    34321 gcgatgtttt gcgctttttt accaccgaaa ccacagaacc tgatcgtact ttgcagttat
    34381 ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga
    34441 gtctgaccgt cgatgagagc gatctttctg tgcatctggt aggacgaaaa atcaataaac
    34501 gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcagttgct cgtgatttat
    34561 cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc
    34621 tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac actgggttga
    34681 aagaacacga cgtcattggc caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg
    34741 cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac
    34801 tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa ataaatttgt
    34861 ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg acattaccga
    34921 agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta tcaccggact
    34981 gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcagg ctgataacaa
    35041 taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg acgcctatgg
    35101 gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa gctgtctcga
    35161 acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg catccaatac
    35221 ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac gcctcccctt
    35281 tcgtattggt ttaattgaag tttataccag ctgttcagta ggtatttcgc tctctcccga
    35341 acatggttcg gacagcgcga ctattattcg tcacgccgac acagcaatgt acacagcgaa
    35401 ggaaggcgga cgaggacaat tttgcgtttt tactccagaa atgaatcagc gggtatttga
    35461 atatctctgg ctggatacca acttgcgtaa agcactggaa aacgatcagt tggttattca
    35521 ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg
    35581 gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg ccgaagagtc
    35641 agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa
    35701 gtggcgggat aaaggcataa acctgcgagt ggcggtaaat atttctgcac gtcagctcgc
    35761 cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt ttgaatactg
    35821 ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac tggcactgtc
    35881 tgttattcaa caatttagcc aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg
    35941 ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt
    36001 ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc
    36061 tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga
    36121 tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg ccaaaccgat
    36181 gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat aagtagatga
    36241 atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat gataattagc
    36301 cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt ttccactttt
    36361 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    36421 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    36481 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    36541 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    36601 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    36661 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    36721 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    36781 tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa acccgtatga
    36841 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg gttcaaccgg
    36901 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctggggg
    36961 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    37021 acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    37081 ttggcccgtt gacacgccag tgcatccaac aggtgcattt cagcaaagca tttattggta
    37141 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    37201 tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    37261 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    37321 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg
    37381 ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc
    37441 taagatgatt cctggttgat aattaagact atttacctgt tattaacacg cttaagatat
    37501 aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat aaccctgcgc
    37561 gcgagcagat ttcacggaat aatttcacca ggcttattct tagctattat agttatagag
    37621 agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac gagcaaaaaa
    37681 atgaccgcgg ctgttctggc aattactttg gcaatgtctc tgagtgcctg ttctaactgg
    37741 tctaaacggg accgcaacac cgcaatcggc gcgggtgcag gggcattagg cggtgcagta
    37801 ctgaccgatg gcagtacgtt gggtacatta ggtggtgcag ctgtcggtgg tgttattggt
    37861 caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat tataatacgc
    37921 ttgattctgt tcagtacgaa ataactgaat aatacgcaca gtaaaatatt cgaaagccac
    37981 ggatatatcc gtggcttttt atgttaaccg cctgcgagtt ttaccttcat ccctttcgct
    38041 tcaagtagtg attttaataa atcacgctta tcgccctgga tttcaataac tccatcttta
    38101 actgctccgc cgcagccgca ttttttcttc agttccgctg caagttttgt cagttcggca
    38161 tcatcgagat cgacaccggt aatcaggcaa acgcccttac ctttacgtcc actggtctga
    38221 cgctgaatac gcaccacacc gtcgccttta gggcgtacag gggccgcttt gggctcatca
    38281 atacgtccgg tttccgttga gtagaccaga cggctgttgg aatcactcat catgcactcc
    38341 gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg atctaccgat tgcgttaccg
    38401 ggcgaccaat caccatataa tcaacaccag ccgacaacgc ctgttctggc gtcataatgc
    38461 ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggcgtaacc agtttgaact
    38521 cctgaccgaa tacctgttta aagcgcacag cttcctgagc agaacacacc acaccatcaa
    38581 ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt gacagtgtca
    38641 tgccaagatc gaccaggtcg ctggcttcca tgctggtcaa cactgtcaca gcaatcaaaa
    38701 gcggtgcatc tttgccaaac ggaaccagtg cctcacgcgc tgcggtcatc atacgcgccc
    38761 caccagaggc atgaacattc accatccaca cgcctaagtc agctgcagca gcgacagcgt
    38821 gcgctgcagt gttaggaata tcgtggaatt tcaggtcaag aaagatatca aaaccacgct
    38881 gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa catctctttg ccgaccttca
    38941 gacgacaatc gcgtgggtcg atcttgtcga caaaggacag cgcgtcatca cgattatgat
    39001 aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat gaagcagtta
    39061 acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg
    39121 cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttttcccc tggcaggctt
    39181 tttctgctcc gtcgttgccg gagcacaatg gaacatcaat aactagtatg ttgtaactaa
    39241 agtcggattt ttttaaaaat tacaggccat caagaccgcg aatcggttta atggttgacc
    39301 aggcccgaca agacggacaa tgccagtaga gggtgtatgc ggtaaaacca catttctggc
    39361 agcgataacg aggcttacta cgtacctttt cgccaaccat gtcacgcagc accatcaggc
    39421 tctctttggc acgcccttct tccgcttcat ttaagtggta atccattaac ttatggaaca
    39481 cacgcatggt cggatgacgc tgaagctggc gcgtaatata gacctgtgcg gcctcactac
    39541 cgtcgcgcgc ttcgatgata tcagcaagca tcaattcagc atcggcaccg gtgttctctt
    39601 ccaccgcgcg ttgcaggaat tctgcccatt cggcagtttt acccaactgc tgatagcagg
    39661 tttgcaacat ttccagcgtt tcgctgacca gttctctgtc ctgggatatc acgcgttgca
    39721 aactttcgac ggctttggcg tattctcctt tcgccataaa cacgcgtccc atcatgatgg
    39781 atacgcgggc gctgttttta tctgccgccg cccctttttt tagcaaggtc atggcacgat
    39841 cgagatcgtc gctggccata tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga
    39901 cgcgctgttt atctttaccc agcttcacca ggcgttcggc aacatcaatt gctttctgcc
    39961 actcgctggt agcctggtag atttgtagca actgttgcag cgcgccaatg cggaagtcag
    40021 tttcatcggt cagctgattg aacatgtctt ccgcgcggtc gtataacccg gcggccatgt
    40081 aatcacgccc cagttgttga atcgccaaca gacgctgttc ataggtcagt gaggcgcttt
    40141 ccattagggt ctgatggatg cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt
    40201 ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc tttaagcata tcgagaaaca
    40261 ggtctaccgc tttatcctgt tgattactaa gcaggaagtt aacccccgct acgtaatcac
    40321 gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc acttctgcgg cccatatacc
    40381 agccataggc agcggctaca ggcaaaagca gaaacaacaa ctccagcata gaaagttatt
    40441 ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt cgcgggtgaa agctggtttt
    40501 ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac acgaactcgc agccagaaca
    40561 ggccacaaat caaccaaccg atagcaaccc ccgcagcaaa caataccgcc agcaatgtgg
    40621 aaatacggta ctccccttgc gctaacagat aattaaacgt cacctgttga tcgttctgcg
    40681 cacccaacgt gaccgaaatc acgaagatcg ctaacaccag taagaaaatg agtaaatatt
    40741 tcacattact tcccgttatg tggttaaagc gaataaatta tggtcaactt accgcaatag
    40801 ccctttaaag taccattttc gcgccgtctg cgaaatggca tccacgcgca ttgttgatga
    40861 tttaagggct aaattcggaa aatcaaccag cgttaacttt cttgttctcg ttgcgctatt
    40921 tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt
    40981 gccaccgcca ccagcgccca cgaaatcaac gtagctacta ccagatcgcg tggccaatgc
    41041 atcccgagca gcaggcggct tcccatgact cccgttgccc agaccagcaa gatagcaatg
    41101 gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca gcagtgccca actggcagca
    41161 aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt tctgccagtg tgaacgcaaa
    41221 tattgtggga tatttttctc ttcagccaac tgttctttca ctagatttcc gcgttctgct
    41281 cgctttaaag tgtagaactc atcaaccgga atatgatgtg ttttttccag ccagataaca
    41341 aaaggtcgtg gttcctggac tttgtctttg atccaggatt taacgccttg tcccacaatg
    41401 attacggccg ccagaatggc aaataataca aaggcagcct taatgcaaaa acgcagacac
    41461 cagagaaacc agccgaataa aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt
    41521 tcagtaaccc aaaaagccgc ttttagtagc caactttgtt ctccaggttg ccaacgccag
    41581 ccagaaatcc atacggctac tggcatgaca agcaatagtg cagctcccac tgcggtacgt
    41641 ctggcaatcg aacgcatggc ctctcctttt tgataagtcc cacaatcata actgaaaacg
    41701 ccagttccag gaaaaattga cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat
    41761 taggtgaacc ccttctcgtt atggcaaaat aagccaatac agaaccagca ttatctggag
    41821 aatttcatgc agcttaaacg tgtggcagaa gccaaactgc caaccccatg gggcgatttc
    41881 ctgatggtgg gatttgaaga actggcaacc ggacacgatc atgtcgcgct agtctatggc
    41941 gatatttccg ggcatacccc ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac
    42001 gccctgttca gcttgcgctg cgattgtggc ttccagctcg aagcggcatt gacgcaaatt
    42061 gccgaggaag gccgtggtat tttgctgtat caccgtcagg aaggtcgtaa cattggtctg
    42121 ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt acgataccgt agaggctaac
    42181 catcagttag gcttcgccgc tgatgagcgc gacttcactc tttgcgctga tatgttcaaa
    42241 ctccttggcg tcaatgaagt ccgcttgtta accaataacc cgaaaaaagt cgaaattctg
    42301 accgaagcag ggattaatat tgttgaacgc gtaccattga ttgtaggtcg taaccccaat
    42361 aacgaacatt atctcgatac caaagccgag aaaatgggtc atttgctgaa caaataaccc
    42421 tctcgcattg tggaattaat ttgcttgccg gaagcaaaat aaccggcaag caaatatttg
    42481 ttacttcagc atattacgaa tgacataatg caaaatgccg tcgttctggt agtaggtcaa
    42541 ctccgtcgcg gtgtcgatac gacaacggca gggtacgacc tcctggctac catccgcgcg
    42601 cgtaagcgtc accggaaccg tcgcaccggg ttgtagattt tgcagatcgc caatatcaat
    42661 cttctcttcc ccggttagtc ctaacgtttt acgcgttacg ccttgcggaa attccagcgg
    42721 caggatgccc atgccaatta aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac
    42781 cacacgaatt ccaagcagac gaggaccttt tgccgcccag tcacggcttg agcctgaacc
    42841 atactctttc ccggcaatca ccgccagcgg cgtttgttcc tgcttatagc gcatcgcagc
    42901 atcataaata gagactacgt cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc
    42961 aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg
    43021 gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc tcaacacccc gaccttgtag
    43081 atatcgaccc gctgggctgt cgggcttaat actgcccgcc ggagagatat ggtcagtggt
    43141 gactgaatcc cccagcattg cgaggatccg cgcaccgtga atatcttcca ctggtgctgg
    43201 tgttgcctgc atttcatcaa agaaaggcga taagcgaata taggttgagt cctcctgcca
    43261 accgtaggta tcggatcgtg tgacgttaat ttccttccac tctgctgtgc cttcaaaaac
    43321 ttctgcgtac tctttgcgga acatttctgt ggagacttgt tctaccgcac gggcaatttc
    43381 ttgtgccgat ggccagatat ctttcagata aaccggatcg cctttacgat catgaccaat
    43441 aggctcagaa gccaggttga tattcatatt tcccgccagc gcataggcaa ccaccagcgg
    43501 cggcgaggcc agccagttgg ttttaaccag cggatggata cggccttcaa agttacggtt
    43561 gccggacagc actgcaccga cggttaaatc gccttttttg attgccgttt cgataggatc
    43621 gggcagcggc ccagagttac caatacaggt agtacaaccg tatcccacaa ggttaaaccc
    43681 cagttcgtcg agaaacggtg tcagtttcgc ttttgccaga tagtcagaaa caactttcga
    43741 gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg aggccaagaa ttacggcttt
    43801 tttcgccagc aagcctgcag ccatcagcac acttgggtta gaggtgttgg tgcacgaggt
    43861 tatcgcagca atgaccacag cgccatcagg taactgatac tgatgtccgt tcataacata
    43921 atcgaccggc tggcgatctt tatgcgtggc attcacttcc agttcgttac tggcggcaaa
    43981 tacttttggt acatcgggca gtgcaacgcg atcctgtggg cgttttggcc ctgccaggct
    44041 cgcttcaacg tcattcatat ccagttctaa cgtactggta aaaatcggtt catcgcccgg
    44101 gttacgccac atgccctgcg ctttggcata tttttcgacc aactcaacct gatcttcgct
    44161 gcgcccgctt aaacgcatgt aatcgagggt tacagcatcg attgggaaga agccacaggt
    44221 ggcaccatat tctggcgaca tattggcaat ggtggcgcga tccgccagcg gtagtgaatc
    44281 cagaccatca ccataaaatt cgacgaattt ctccaccacg ccatgtttgc gcagcatttg
    44341 ggtaacagtg agaaccaggt ctgtggcggt aataccttca cgtaatttac cggtaagttt
    44401 aaagcccact acgtccggga taagcatgga aaccggctgg ccta
//
LOCUS       DAZOXR010000008        44090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZOXR010000008 DAZOXR010000000
VERSION     DAZOXR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.008"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(55..1914)
                     /gene="gss"
                     /locus_tag="ACHUNN_000411"
     CDS             complement(55..1914)
                     /gene="gss"
                     /locus_tag="ACHUNN_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0574889.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2119..2985
                     /gene="yghU"
                     /locus_tag="ACHUNN_000412"
     CDS             2119..2985
                     /gene="yghU"
                     /locus_tag="ACHUNN_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0574890.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3014..3095)
                     /locus_tag="ACHUNN_000413"
                     /pseudo
     CDS             complement(3014..3095)
                     /locus_tag="ACHUNN_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3108..3356)
                     /gene="hybG"
                     /locus_tag="ACHUNN_000414"
     CDS             complement(3108..3356)
                     /gene="hybG"
                     /locus_tag="ACHUNN_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0574891.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3369..3710)
                     /gene="hypA"
                     /locus_tag="ACHUNN_000415"
     CDS             complement(3369..3710)
                     /gene="hypA"
                     /locus_tag="ACHUNN_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0574892.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3703..4191)
                     /gene="hybE"
                     /locus_tag="ACHUNN_000416"
     CDS             complement(3703..4191)
                     /gene="hybE"
                     /locus_tag="ACHUNN_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0574893.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4184..4678)
                     /locus_tag="ACHUNN_000417"
     CDS             complement(4184..4678)
                     /locus_tag="ACHUNN_000417"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0574894.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4678..6381)
                     /gene="hybC"
                     /locus_tag="ACHUNN_000418"
     CDS             complement(4678..6381)
                     /gene="hybC"
                     /locus_tag="ACHUNN_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0574895.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6378..7556)
                     /gene="hybB"
                     /locus_tag="ACHUNN_000419"
     CDS             complement(6378..7556)
                     /gene="hybB"
                     /locus_tag="ACHUNN_000419"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0574896.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7546..8532)
                     /gene="hybA"
                     /locus_tag="ACHUNN_000420"
     CDS             complement(7546..8532)
                     /gene="hybA"
                     /locus_tag="ACHUNN_000420"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0574897.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8535..9653)
                     /gene="hybO"
                     /locus_tag="ACHUNN_000421"
     CDS             complement(8535..9653)
                     /gene="hybO"
                     /locus_tag="ACHUNN_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0574898.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9842..10129)
                     /gene="yghW"
                     /locus_tag="ACHUNN_000422"
     CDS             complement(9842..10129)
                     /gene="yghW"
                     /locus_tag="ACHUNN_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0574899.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10248..11135)
                     /gene="yghX"
                     /locus_tag="ACHUNN_000423"
     CDS             complement(10248..11135)
                     /gene="yghX"
                     /locus_tag="ACHUNN_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0574900.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11292..12332
                     /gene="gpr"
                     /locus_tag="ACHUNN_000424"
     CDS             11292..12332
                     /gene="gpr"
                     /locus_tag="ACHUNN_000424"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0574901.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12372..12866)
                     /locus_tag="ACHUNN_000425"
     CDS             complement(12372..12866)
                     /locus_tag="ACHUNN_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0574902.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13057..13941
                     /gene="yghA"
                     /locus_tag="ACHUNN_000426"
     CDS             13057..13941
                     /gene="yghA"
                     /locus_tag="ACHUNN_000426"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0574903.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14101..14526)
                     /gene="exbD"
                     /locus_tag="ACHUNN_000427"
     CDS             complement(14101..14526)
                     /gene="exbD"
                     /locus_tag="ACHUNN_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0574904.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14533..15267)
                     /gene="exbB"
                     /locus_tag="ACHUNN_000428"
     CDS             complement(14533..15267)
                     /gene="exbB"
                     /locus_tag="ACHUNN_000428"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0574905.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15519..16706
                     /gene="metC"
                     /locus_tag="ACHUNN_000429"
     CDS             15519..16706
                     /gene="metC"
                     /locus_tag="ACHUNN_000429"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0574906.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16846..17505
                     /gene="yghB"
                     /locus_tag="ACHUNN_000430"
     CDS             16846..17505
                     /gene="yghB"
                     /locus_tag="ACHUNN_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0574907.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17545..18444)
                     /gene="yqhC"
                     /locus_tag="ACHUNN_000431"
     CDS             complement(17545..18444)
                     /gene="yqhC"
                     /locus_tag="ACHUNN_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0574908.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18638..19801
                     /gene="yqhD"
                     /locus_tag="ACHUNN_000432"
     CDS             18638..19801
                     /gene="yqhD"
                     /locus_tag="ACHUNN_000432"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0574909.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19906..20733
                     /gene="dkgA"
                     /locus_tag="ACHUNN_000433"
     CDS             19906..20733
                     /gene="dkgA"
                     /locus_tag="ACHUNN_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0574910.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20933..21859
                     /locus_tag="ACHUNN_000434"
     CDS             20933..21859
                     /locus_tag="ACHUNN_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0574911.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21910..22167
                     /gene="yqhH"
                     /locus_tag="ACHUNN_000435"
     CDS             21910..22167
                     /gene="yqhH"
                     /locus_tag="ACHUNN_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0574912.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22210..24429)
                     /locus_tag="ACHUNN_000436"
     CDS             complement(22210..24429)
                     /locus_tag="ACHUNN_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0574913.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24540..25952)
                     /gene="ftsP"
                     /locus_tag="ACHUNN_000437"
     CDS             complement(24540..25952)
                     /gene="ftsP"
                     /locus_tag="ACHUNN_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0574914.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26027..26764)
                     /gene="plsC"
                     /locus_tag="ACHUNN_000438"
                     /gene_synonym="parF"
     CDS             complement(26027..26764)
                     /gene="plsC"
                     /locus_tag="ACHUNN_000438"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0574915.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26997..29255)
                     /gene="parC"
                     /locus_tag="ACHUNN_000439"
     CDS             complement(26997..29255)
                     /gene="parC"
                     /locus_tag="ACHUNN_000439"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0574916.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29801..30283)
                     /locus_tag="ACHUNN_000440"
     CDS             complement(29801..30283)
                     /locus_tag="ACHUNN_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0574917.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30336..30728)
                     /gene="ygiW"
                     /locus_tag="ACHUNN_000441"
     CDS             complement(30336..30728)
                     /gene="ygiW"
                     /locus_tag="ACHUNN_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0574918.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30880..31539
                     /gene="qseB"
                     /locus_tag="ACHUNN_000442"
     CDS             30880..31539
                     /gene="qseB"
                     /locus_tag="ACHUNN_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0574919.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31536..32885
                     /gene="qseC"
                     /locus_tag="ACHUNN_000443"
     CDS             31536..32885
                     /gene="qseC"
                     /locus_tag="ACHUNN_000443"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0574920.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32995..33576
                     /gene="mdaB"
                     /locus_tag="ACHUNN_000444"
     CDS             32995..33576
                     /gene="mdaB"
                     /locus_tag="ACHUNN_000444"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0574921.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33607..33921
                     /locus_tag="ACHUNN_000445"
     CDS             33607..33921
                     /locus_tag="ACHUNN_000445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0574922.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33969..35861)
                     /gene="parE"
                     /locus_tag="ACHUNN_000446"
     CDS             complement(33969..35861)
                     /gene="parE"
                     /locus_tag="ACHUNN_000446"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0574923.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35890..36471)
                     /gene="yqiA"
                     /locus_tag="ACHUNN_000447"
     CDS             complement(35890..36471)
                     /gene="yqiA"
                     /locus_tag="ACHUNN_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0574924.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36471..37298)
                     /gene="cpdA"
                     /locus_tag="ACHUNN_000448"
     CDS             complement(36471..37298)
                     /gene="cpdA"
                     /locus_tag="ACHUNN_000448"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0574925.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37323..37745)
                     /locus_tag="ACHUNN_000449"
     CDS             complement(37323..37745)
                     /locus_tag="ACHUNN_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0574926.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37746..38375)
                     /gene="nudF"
                     /locus_tag="ACHUNN_000450"
     CDS             complement(37746..38375)
                     /gene="nudF"
                     /locus_tag="ACHUNN_000450"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0574927.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38580..40061
                     /gene="tolC"
                     /locus_tag="ACHUNN_000451"
     CDS             38580..40061
                     /gene="tolC"
                     /locus_tag="ACHUNN_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0574928.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40209..40880
                     /locus_tag="ACHUNN_000452"
     CDS             40209..40880
                     /locus_tag="ACHUNN_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0574929.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40886..42046
                     /locus_tag="ACHUNN_000453"
     CDS             40886..42046
                     /locus_tag="ACHUNN_000453"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0574930.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42084..42872)
                     /gene="ygiD"
                     /locus_tag="ACHUNN_000454"
     CDS             complement(42084..42872)
                     /gene="ygiD"
                     /locus_tag="ACHUNN_000454"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0574931.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43015..43788
                     /gene="zupT"
                     /locus_tag="ACHUNN_000455"
     CDS             43015..43788
                     /gene="zupT"
                     /locus_tag="ACHUNN_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0574932.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43846..44016)
                     /gene="yqiD"
                     /locus_tag="ACHUNN_000456"
     CDS             complement(43846..44016)
                     /gene="yqiD"
                     /locus_tag="ACHUNN_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0574933.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT        10823 a        11852 c        11108 g        10307 t
ORIGIN      
        1 taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca tgtattactt
       61 tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg attcatcacc
      121 gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag gtacatacct gaatgtattt
      181 accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct cggcaaattt
      241 accgctggtt ttgtccagca cctcttcatg atggctgacc aggtcgatat tgctaccaca
      301 gcgaccggca atcggtttca ctgcgtaacc tgttttcacc aattcatcat taacagtgaa
      361 atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg gcagaatcgc
      421 tttgttgccg gggatcaccg tccacagcgg ctcaaagact agcacttccg ggcgcagcaa
      481 tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca ccgcagcaaa
      541 ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc aggttttcca
      601 cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat cccagcccag
      661 ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc tggtgcagcg cctgctccat
      721 aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga caaacggacg
      781 tgcacgactg tgtttccagg caccagccaa ttcgttaatc agcccttccg ccggattgaa
      841 gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg cttcggtatg
      901 acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat ccatgcagaa
      961 atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac gcaaacgtgg
     1021 ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca gcaccttgtc
     1081 ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct cctgctcggc
     1141 actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac cgttggcctg
     1201 cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa actggccttt
     1261 gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg gctgcggtaa
     1321 gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt catcaaaagt
     1381 gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcacgcg tccactgttg
     1441 cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt tgttgccatg
     1501 caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt tatcccagat
     1561 aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct gcaatggcag
     1621 gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc acgccatacc
     1681 cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa attcaacgca
     1741 ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg cgtcatcttc
     1801 gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc cacctggggc
     1861 gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc tcatcacttt
     1921 acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt acctgctcag
     1981 cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg ctggcgcaaa
     2041 gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc aacactacca
     2101 ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt ctggacgtgg
     2161 gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg tccgacgcat
     2221 gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg aacgccgaac
     2281 ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac tggtgcagag
     2341 tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg ctttgtcgaa
     2401 gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc gccgatccgc
     2461 gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta cttcctgccg
     2521 caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca gggcgcggca
     2581 ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccgatgaa aattgagtac
     2641 gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga taagcaactg
     2701 gcgcagcata agtttgttgc gggcgatgag tacaccattg cggatatggc gatttggccg
     2761 tggtttggca atgtggtgtt aggcggcgtg tatgatgccg cagagtttct tgatgcgggc
     2821 agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc ggtgaaacgt
     2881 gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga gcgccatgac
     2941 gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt ggtgttcgtc
     3001 gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc gtgcacaatg
     3061 cctgatgcga cgctgccgcg tcttatcagg cctacgaaag ctaatcatca cgcgctggta
     3121 atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg
     3181 atgctcatgg caaatccgac gtgtaccagc acccactggc ccagtagatc ggcagggtta
     3241 ccttcacaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc aacctgcgca
     3301 agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat acacattatt
     3361 aactccggtt attcaacttc aatacttttg acgatcagcg aatcgccggt atcgacacgc
     3421 aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc caccacctgg
     3481 ctgcaatccc agcaccaagc ctgggcgggt ttatagacga tatgtaaatc gcacccttgc
     3541 gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc ctcaacgcag
     3601 gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc
     3661 tgccgttgga taatttcaac ggcgctctgg caaagagaca actcatgcat tttcgccact
     3721 cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat tcgtgactgg
     3781 cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt caatcgacat
     3841 gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat tgcgaaacac cgtccagttc
     3901 gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt cactcacttt
     3961 gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca tccacggggt
     4021 gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat acaccggcat
     4081 tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt caaacgctgc
     4141 ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca tgaatcgcct
     4201 cccgtgggat ggcttccacg cctgattcgc gcagcgcagc cagaacctgc tcaagcgcag
     4261 gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc gattccggga
     4321 tcacgccgac cagggtcagc tttttcggaa actcaccggt gaagcgcagg gccgacaata
     4381 cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc ggaacttctt
     4441 catcccgcag gatcatcatc gttcccggcg tgttcttttt cgacacaatg gcatccgcaa
     4501 taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc gtgccgccat
     4561 cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg acaatccgca
     4621 caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa atacgcatta
     4681 cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta ccgcacaggc
     4741 catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat tcggatcagc
     4801 aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga agttacgcgg
     4861 accagagttc caggttgatg gaacaactgc ctggtagtta ctgataatac cgtctttaat
     4921 aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac ctttgaactc
     4981 acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga tcgcctttgc cgatattggt
     5041 gatcagtgca ctgtattgat tttgcaggat atcctgcaat tcgcagcagt gaacggtacg
     5101 accaataata cggcccagcg tggagtgcag ctgcgccact tccagcgtgt tgccagtcag
     5161 tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc gacctgccgc
     5221 cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt agaaagtcgg
     5281 tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg gaatggtggt
     5341 accttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg cgctttcctg
     5401 aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg aggacagatc
     5461 cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttactgt cggtcgggaa
     5521 ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt ccgggtagaa
     5581 cgcggcaata actgcggtat caaccttata aacctgttca acaaagtcgc tcagcttgtc
     5641 gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac cgtcgaggtt
     5701 gatcgggttc gcgacaccac ctaccgccag gttctgaatg tgcggcgttt taccgcccag
     5761 cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt agtgcgctac
     5821 cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgaccccagt agccattggc
     5881 gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa ctttagtgaa
     5941 ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcgg aggctttggt
     6001 tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa gctgatagaa
     6061 atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga tgtattgcgc
     6121 gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag acagcgcgtg
     6181 agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc gcggatcgcg
     6241 gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg ctttcgaaac
     6301 gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg ttaccggatc
     6361 aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc gttttgtttt
     6421 aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat ctcaatagcc
     6481 acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta accgccgccc
     6541 gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa cagtgcgctc
     6601 agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg cagaacgacc
     6661 agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa gtcaccggca
     6721 aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac gatgaaaatc
     6781 gccagcaaca cactgatggt gttggtcagc ttgacgaaca gactcttttc atccggaccg
     6841 ttgccacgca gacccgcctg caccagcgaa ccttcaaaga tgacaatcga gaagcccatg
     6901 atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca caacggatgc
     6961 accttgtagc ccgccgagat catcagtgac cccattgaag actggtgcat ggtcggcaac
     7021 agcgcgccaa gtgcaatgat gaagaacata accttgttca gacgcttgag agaaaccttc
     7081 cagcccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc gatatagatg
     7141 gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc cggaatgtag
     7201 aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc acccagtgag
     7261 taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg cccacggtta
     7321 aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt gccaatcagc
     7381 aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga gacagagccc
     7441 agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa cggtccaaaa
     7501 atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg actcatgatc
     7561 gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg ttaagcccgc
     7621 cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat tttcggaacg
     7681 cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag gcacacccgt
     7741 cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg gataatattt
     7801 cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat agtggtattc
     7861 gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt cacgcgtacc
     7921 gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc ctttatcgag
     7981 acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa acgggttgtt
     8041 gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc aaccggtgca
     8101 cacgtctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg cagagaccgg
     8161 gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta
     8221 gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct gaatgatgtt
     8281 attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg
     8341 ttcagggaag ttgatatcct gacacttggt gacgcaagcc tggcagccta cgcacaaggt
     8401 cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag cagccgcatg
     8461 actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt taataaaatt
     8521 acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg ctgacgaccc
     8581 agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccacacc gccgagcaaa
     8641 ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg tttctgtgaa
     8701 cgcggagtct gattttcgac gttggcaagc tgatggatgc ctttatggaa gccgatacct
     8761 tcttcgttac agccatagca tgggtgacca atcgctaccg gccacacgcc gccaacatcg
     8821 cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc gaggtggtac
     8881 aggcaccagc cttcacggtg gccttcatca ccgaactctt tggcaaaacg accagcatcg
     8941 aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa ggtcggacgg
     9001 tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac ggtcgcgagg
     9061 aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc tggcagaact
     9121 tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc gccccacgca
     9181 gagcaggaac cgatagcgat aatcgctgct gcgccttctg ccgctttgcg gatgtgatcc
     9241 acaatcggct caccagcaac catgcaataa ataccgttat cttttaatgg gatggaacca
     9301 tccacaacca acacatactg ccctttgtac ttctcaagtg cgttatgttt gttctcttcg
     9361 acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat agtctccagc
     9421 acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca accggtgcac
     9481 tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga ttcggccatc
     9541 tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca aagcttcatg
     9601 aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt catttatagt
     9661 tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc
     9721 gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg cggcggggcg
     9781 ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata cgtatgtttg
     9841 atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag gaattattct
     9901 cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc agaatacccg
     9961 cttcccaggc gctaagctgg cgatctccgg tcttttcgta catccggtgt gagtagccta
    10021 atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca ctgtcggcat
    10081 gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc gttcacctca
    10141 ttcgctatat atcgccatat atagcgatat tttaggcaaa gaacgacttt tgcgcacgct
    10201 attttttcat gcttgtttaa gacgtaatcc agtaagagat aaacctatca ggagagatat
    10261 ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt gtcataacgg
    10321 ggcgtggaat cattatggaa tccgtgatta acccccggat agatatacgc ctcataaacc
    10381 ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat tcgggtgtcc
    10441 agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc
    10501 tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc cgccgcgttc
    10561 gatacgccac cgccatagca aaatccggta atacccactt tgcctgccgc ttgtggatag
    10621 cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg atcaacctgc
    10681 tgttgcagct cacgaccttt atcatcattt cccggataac ctccaacgga acttaagccg
    10741 tcaggtgcca gggcgatata ccccgccttc gccactcgcc gtgccacatc ttcgatatac
    10801 ggattcagtc cacgattctc atgcaccacc accacggctg gcgttttgcc gctcattttt
    10861 gcgggtttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg atacatgatg
    10921 tactcagcaa caatctccgg gtcggtaaac tctacctgag tcgccagcgc ataatttggc
    10981 ttgagcaaat caaacaacgc taatgccgtc atcccgccca ccgcatactt cgccgcaagg
    11041 ttgaggaact cacgtttcga aattttcccg tgagcgtaat agtcgtagta atccaacaac
    11101 tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cactcctcag ttggtgtttt
    11161 tttaagcaaa gcataagcac gtgtttttgc ccagtttttc gtcactctgt gagccagact
    11221 acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt gaacggtgga
    11281 aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg caataccgct
    11341 attgcggaaa aagcggttta cgcctgcccg cgttatcgct cggtttatgg cacaatttcg
    11401 gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt gatttgggca
    11461 ttacgcactt tgatttagcc aacaattacg ggccgcctcc aggaagcgca gaagagaact
    11521 ttggtcgcct gctgcgggag gattttgccg cttatcgcga tgaactgatt atctctacca
    11581 aggctggcta cgatatgtgg cccggccctt acggctctgg cggttcacgt aaatacctgc
    11641 tcgccagcct cgaccaaagc ctgaagcgta tagggcttga gtatgtcgat atcttttact
    11701 ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg gctcatgcgg
    11761 tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag cggacgcaaa
    11821 aaatggtcga gttgctgcac gagtggaaaa ttccgctttt aattcatcaa ccttcgtaca
    11881 atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa aataacggcg
    11941 tgggctgtat tgcctttact cctctggctc agggattgct gaccggaaaa tatctcaacg
    12001 gcattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt ctgacgccga
    12061 aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg gcacagcagc
    12121 gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat cgcgtgacgt
    12181 cggtattgat tggtgccagc cgcgcggagc aactagagga gaacgtgcag gcgctgaata
    12241 atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc gatggcgagc
    12301 tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa taacgggcgc gcgaaccgcg
    12361 cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat cacaaatgca
    12421 gaaagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc cgggacattt
    12481 ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat cgacgctgct
    12541 actttgtttt tcagcgacgt tgcgtccatt ttccccagcc agttcagctt ctctttgttc
    12601 tcggagatat ccagctgtga gacgaaattc tcataaccgg aaaacatcac catcaccagc
    12661 aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa atctgattcc
    12721 gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt cagcgccagg
    12781 gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg agaagcatac
    12841 attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac gggcaacagt
    12901 atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta aacctttgtc
    12961 gcataattct gaggtttagg gatcttcctg gttcccttat caacgcatcg agagcgtcta
    13021 cccttaaagg acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa agacccgacc
    13081 acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg catccaggcg
    13141 aagatgacac cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg tcgcctgaaa
    13201 gatcgtaaag cactggtgac agggggcgat tccggaatag gtcgcgctgc cgccatcgct
    13261 tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga agaagacgct
    13321 caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct gccaggcgat
    13381 ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc gttaggcggg
    13441 ctggatatta tggcgctggt cgccgggaaa caggttgcca ttccggatat tgcagacctc
    13501 accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt ctggctaacc
    13561 caggaagcga tccccctgct accgaaaggt gcaagtatca tcaccacttc gtcaatccag
    13621 gcataccagc caagtccgca tttactggac tatgcggcta cgaaggcggc gattctgaac
    13681 tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg
    13741 ccaggcccta tctggacggc actgcaaatt tccggcggac aaacgcagga taagatcccg
    13801 cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggaact ggcccctgta
    13861 tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca cggcgtgtgc
    13921 ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg gcatcattgt
    13981 gaattcactc ttacctgatg tggcgaaaac gccttatccg gcctacaaac gcacgaagat
    14041 tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg caattctact
    14101 ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac ccgcctgatg
    14161 cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttgtccg ctcggaagaa
    14221 gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca ttgtttcatc
    14281 ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca gataaaccgg
    14341 tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct tcacatctac
    14401 cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca cgtcgataaa
    14461 cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt taagatgcat
    14521 tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat gcgcagcggc
    14581 gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg caacatcacc
    14641 cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa cgaccgcagg
    14701 aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac ccggcgcaac
    14761 gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca tgatgcccca
    14821 gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca gatagccgtt
    14881 accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga agctggtacg
    14941 ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat tctgcgcttc
    15001 attgagcaaa tgcaggctta agcttttgct accaaaatca gcggcgatat cgttggcctg
    15061 gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaaggcgac gcttctgatt
    15121 gaagaactct acgctcttac tgaagaagat tgcccaagtg actacggagg ccaaaataag
    15181 cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc agacggaaag
    15241 gtccgtctgc atcaaattat tacccactgt gtatctccag gacgcaagtc acaaaatctg
    15301 cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt ctcattacta tttgcatact
    15361 gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc agcacgccat
    15421 ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat attcatgcta
    15481 gtttagacat ccagacgtat aaaaacagga atcccgagat gacggacaaa aagcttgata
    15541 ctcaactggt gaatgcagga cgcagcaaaa aatacactct cggcgcggta aatagcgtga
    15601 ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc caaaaagcac gcgacgcgca
    15661 atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat ttctccttac
    15721 aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt gctatttccc tgcggggcgg
    15781 cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg ttgatgacca
    15841 acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa ctgggcgtaa
    15901 cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg cagccaaaca
    15961 ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat ggaagtccac gacgttccgg
    16021 cgattgttgc cgccgtacgc agtgtggtgc cggatgccat cattatgatc gacaacacct
    16081 gggcagccgg tgtgctgttt aaggcgctgg actttggcat cgatgtttct attcaagccg
    16141 ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgta tgcaatgccc
    16201 gttgctggga gcagctacgg gaaaacgcct atctgatggg ccagatggtc gatgccgata
    16261 ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt caacatcatg
    16321 aaagcagtct gaaagtggct gaatggctgg ctgaacatcc gcaagtagcg cgagttaacc
    16381 accctgctct gcctggcagt aaaggccacg aattctggaa acgagacttt acaggcagca
    16441 gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa tgaagagctg gcgaactatc
    16501 tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa tcgttgatcc
    16561 tggcgaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc gattttagcg
    16621 ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt gccgatctgg
    16681 acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg cagacattta
    16741 attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg aatcaggagt
    16801 acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc tgttattcaa
    16861 gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc tcatattgtt
    16921 agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct gctgcccgcc
    16981 tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc ccaaggggtt
    17041 atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg ctgctggcta
    17101 agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg gctggcacag
    17161 cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg tctgctggcg
    17221 ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat ggcgggaatt
    17281 tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt gctgtgggtc
    17341 agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa acgccatgaa
    17401 gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc tggcttgtta
    17461 ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg cctgacgatt ttccccgttc
    17521 ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc ttgccgcatc
    17581 ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgcg atgcgctttc
    17641 atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga tcatcatccg
    17701 cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg tgacagactt
    17761 aaaattatga tggaacgccg atacgctcat gttggcttct gccgccagtt gctcgacgct
    17821 caggttttcg gtgtatttat tctcaatccg tttcagcacg cggctaatca gactgaagtg
    17881 agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca gcacgtagta
    17941 cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc gctccatcac
    18001 gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg agttaatccc
    18061 gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca acagttcctg
    18121 taactgcaaa atatcgacat tgagacgcaa tcctgccagc ggcacctctg acgtggcata
    18181 ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg catcataacg
    18241 aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta tgatgccagg
    18301 ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac gcacatcggg
    18361 caacagttct gacaaactat tttctttatt tttcagttta ttaactttat ccgccagcaa
    18421 gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc ggcaatttga
    18481 ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag acattggcag
    18541 aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag gcagatcgtt
    18601 ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta atctgcacac
    18661 cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac aaattcctca
    18721 cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaatcg gcgttctcga
    18781 tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta ttgagccaaa
    18841 cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga aagtgacttt
    18901 cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg ccgcagcggc
    18961 taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta aagagattaa
    19021 aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag aatccaacgc
    19081 aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt ctgcccatgt
    19141 tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc cgcgtcaggt
    19201 ggctaacgac gtagtggacg cctttgtaca caccgtggaa cagtatgtta ccaaaccggt
    19261 tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctaa tcgaagatgg
    19321 tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca tgtgggcggc
    19381 gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg caggactggg caacgcatat
    19441 gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac tggctatcgt
    19501 cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc tgcaatatgc
    19561 tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg ccgcgattgc
    19621 cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg actacggtct
    19681 ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca tgacccaact
    19741 gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag ccgcccgcta
    19801 agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa gttcacaaca
    19861 cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc taatccaacc
    19921 gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt ctggcaagca
    19981 agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta tcgctcgatt
    20041 gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa aaatgcctca
    20101 gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca caagcgcccc
    20161 cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga cctctactta
    20221 atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg catgatcgaa
    20281 ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat ccatcacctg
    20341 caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga acttcatccg
    20401 ctgatgcaac aacgccagct acacgcctgg aacgcgacac acaaaatcca gaccgaatcc
    20461 tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat tcgcgatctg
    20521 gcagataaat acggcaaagc cccggcgcag attgttatcc gctggcatct ggatagcggc
    20581 ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt tgatgtctgg
    20641 gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa aactcgatca gggcaagcgt
    20701 ctcggtcccg atcctgacca gttcggcggc taacatgcaa attctcccgg tgacggtaat
    20761 gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc cgttgttact
    20821 cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg aagctcttaa
    20881 aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac agatgaaaat
    20941 aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc ctccttcaca
    21001 gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg ccagtacacc
    21061 ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac aggcgcttac
    21121 cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg atccggtttg
    21181 tgattgtcag gattttggcg atctggtgct agaaagcgtt gcgataaccc aaactgacgc
    21241 cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag aaaagaccac
    21301 gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata ttgtcagcaa
    21361 tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg ccgctttagc
    21421 ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac atattgctga
    21481 ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg ttaatgcctt
    21541 ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa tagaacggca
    21601 atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac gcgttgatga
    21661 aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt ttacgctgac
    21721 caatggcaac aacgaagagc aagaactggt tttacagcgg cgcgaaggca agtgggaaat
    21781 cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg caaaaaccgc
    21841 cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat aaataaaagg
    21901 gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac ctgcttgctc
    21961 agtggctgcg tcaatgagca aaaggtcaat caactggcga gcaatgtaca aacattaaat
    22021 gccaaaatcg cccggcttga gcaggatata aaagcactac gcccacaaat ctatgctgcc
    22081 aaatccgaag ctaacagagc caatacgcgt cttgatgctc aggactattt tgattgcctg
    22141 cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt cagttgccgg
    22201 ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag cattcaccgg
    22261 acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct gggtcgccat
    22321 cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag cttcacgcat
    22381 ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca gatgcttttt
    22441 gcccatcgct tccagcgccg ggcggattaa cggccagttt gccggatcgt ggtaacgcaa
    22501 caacgcttta tgcaaacgac gctgtttgtc gcccttcggt acgaagacgt cttcgctctt
    22561 ataaccaatc ttcgccagcg gttttttccc ggtgtaatac atggtggttg agttcgccag
    22621 cggcgatggg tagaagttct gtacctggtc gagacggaaa cgatgctttt tcagccacag
    22681 cgccaaattc accatatctt catcacgcgt accggggtgc gcagagatga aatacgggat
    22741 cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt taaagcggtc
    22801 atagctgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg tatgttccgg
    22861 agcaatcttc agataaccgc cgacgtgatg ggtcgccaac tctttgatat agcgcggatc
    22921 ttccacggcg atgtcataac gtacgcctga ggcgatgagg atttttttaa tgcctttcag
    22981 atcacgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca tgtgcggaca
    23041 aatatccgga taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg gcgatttgca
    23101 gtgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc ccgtaaaacc
    23161 tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg aacggctctg
    23221 aataatgcgc ccttcgtgct cggtaataga acagaaagaa cagccgccaa agcagccgcg
    23281 cataatgttg accgaaaaac ggatcatttc gtaagccgga atacgggcat tgccataggc
    23341 cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt cggtagaaag
    23401 cgggatagca ggcgggttga tccacacata gcggtcaccg tgtttttgca tcaatgcgcg
    23461 gacgcagcct gggttggttt cgtggtgcag aatacgcgaa gcatgggcgt acagcacttt
    23521 atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc acggtttcgg
    23581 gcgcggtggc tgcacggtta cggctttggc ttcctgcttt ttcggtgcca ccggtttgtt
    23641 atccgcgcac ggcaaatctt caccatacgg atgcgggatt gggtcgattt ttccaggggt
    23701 atcaagacgg ggggaatcca cgccgctcca gccaggcagc gcctctttca cgataatcgc
    23761 ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac ggtgcgccac
    23821 ctccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg aatccaccag
    23881 cacggaacgg cgcacggtat cggaccagta atcataatgt gcggtacggc gcagactagc
    23941 ctcaataccg ccgaggatca ccggtacatc tttccacgcc tctttacaac gctgggtata
    24001 aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta tccggcgtgt aggcatcgtc
    24061 atgacgtaaa cggcgatcgg cggtataacg gttgatcatc gaatccatgt tgccagcagt
    24121 aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc tgctccagtc
    24181 cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc agcatacgac cgcaaatcgc
    24241 catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga tgtcgcagct
    24301 atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg ccggtccaaa
    24361 acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga tcagggagat
    24421 agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg attataagcc
    24481 ggaacgaaag agaaatcgaa aagtattcca tactcgccct cctcgggcga gtatgaagat
    24541 tacggtaccg gattgactaa cagttgccca atcgagccac ggtccgccat ttccagcgtc
    24601 tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata aacaagcagc
    24661 tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg gaacggcatc
    24721 gcaccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc ttgcggctcg
    24781 tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc aatacggttg
    24841 acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc gcgactgcga
    24901 atcggcgaac cggccatgat ttcagttggc agcaagcgca tcggaagact gtctgtgacc
    24961 agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag aatactggat
    25021 ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcgccaca ggtgatcgac
    25081 acttcatcgc cgttgctcat atccaccaga atctcgcggc gctcgcccgg tgccagcgaa
    25141 agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat cacatgtaac
    25201 gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt cagcagtcgc
    25261 aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt aaccagcagc
    25321 gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc aaagttatcc
    25381 agccgtttat cctggataat gaccggaaaa tcatccacat cataatggtt ggggataggc
    25441 agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt atagacctgc
    25501 tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt ctggcgaata
    25561 ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc gcccatcagc
    25621 gggccaggta cctgtagccc ggccaccgtc attgagacat tttctgtcag gcggttgctg
    25681 taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag gtaacgacca
    25741 ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc acgttgtaca
    25801 gtcataaaca gcggttgccc acggcgagat tcaagtagcg gcggaacggg tagcggttgc
    25861 tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc aatccccgat
    25921 gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac tggctaaagt
    25981 aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa acttttccgg
    26041 cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc attatcgaac
    26101 gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg tcaattggcg
    26161 gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc ttattcgaag
    26221 ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg tgaaatgctc
    26281 cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg aacatccaga
    26341 tggaaatacg gcgttttttg aaatgattca cgacttccgc aatggtgccg tgagctttag
    26401 tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc ccgaagaagg
    26461 ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca atgttcgatg
    26521 ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg ccgtagcttt
    26581 cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagcggcgca agacggccaa
    26641 acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg cagtaaatgg
    26701 agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga aagatatata
    26761 gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa atcaggttag
    26821 ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc atgaataaac
    26881 aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg gatgacgact
    26941 taacgtttca tccggcgttc cttgcaagcg ggaggaaaca ggccctcccc ggcatattac
    27001 tcttcgctat caccgttgct ggcacggcga ggagagtcga tctcaacacg atcgatacgc
    27061 tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac cagtgacttt ctgtaactct
    27121 tccgggcgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc
    27181 agaacgtaca attgcgccag accatcttct ccacgcgcgg cttctgccga tggaatgttg
    27241 ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg gaacatcaac
    27301 atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat caccaccggc
    27361 ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg gttacgcgcc
    27421 accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat cagcagtttc
    27481 tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa cgttaatttg
    27541 ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc gatggcatag
    27601 ctacgaccgg tggaatcaac aaacactacc gattggttgc tcttaccttt caccgccact
    27661 ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg gcctttagcg
    27721 ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg cagcatgtcg
    27781 tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg atcgtcaccg
    27841 taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt acgctcggaa
    27901 gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc accgcgaatc
    27961 ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg
    28021 gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc gttacgaatg
    28081 atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag gatatgcagg
    28141 cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc gcgacggaac
    28201 accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc atccagaccg
    28261 atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa gaggtggttc
    28321 atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg ggtcgggttc
    28381 tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt gcgcatttgc
    28441 gcagcaattt gctccagtac acgcgcacct gaaacctgat gcggtaatgc gctgataacc
    28501 accgcgccat cttctttctt ccacaccgcg cgcatacgta ctgaaccacg tccgttctcg
    28561 tagattttac ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg ataatccggc
    28621 ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat taatgcgatt
    28681 gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat gccgacggca
    28741 ataccggtgg tgccgttaag caaaatgttt ggcagacgag caggcagcat tttcggctcc
    28801 tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag ctcgctcaat
    28861 agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc gaacgatttc
    28921 ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg gtaagagaac
    28981 ggctgcgcca tcaggaccat cgcttcataa caggcgatat cgccgtgcgg atggtattta
    29041 cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc attcaggccc
    29101 agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc accaataaac
    29161 ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt ttccgtaaat
    29221 tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc aactgattcg
    29281 ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg tcgagtcaca
    29341 ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg catcaatgtg
    29401 ccttgatata caacgctttg ctgtagactt gatcggcata gagggcagca caaggttgcg
    29461 cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag ttgaggttat
    29521 gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatatg gttcagccca
    29581 gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc ttgctcatgg
    29641 gtacccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac atggtattcc
    29701 ttaccggaga aattataagc ccaacaacaa tacgcctgtg gcattgttcg ctcaaactta
    29761 tcgcgagtga tttggctcac actccggtaa agattcccca tcacgctaac ggcacataaa
    29821 catccgtttc cagtcgctgc tccgtcaccc cttcggcaag attggtgtag tgaaacagaa
    29881 tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata atgccccata
    29941 tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa cgtccaccgg
    30001 taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta acgctgccgc
    30061 agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag gcgacgccaa
    30121 aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg ataaatttcg
    30181 ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc acagggattg
    30241 atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct ccctgcgaaa
    30301 ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt gcggatctgt
    30361 ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat ctcaaccgta
    30421 tctttcggcg tcaccgtcac accgttccag cgtttgtggt cgatatcaac attgatagta
    30481 ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat attgccgcgc
    30541 agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt tacgctgccg
    30601 ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga aaaaccgccc
    30661 tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac tgctgcgaat
    30721 tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa gatagtcctt
    30781 aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt tacaacacgg
    30841 tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt actgatagaa
    30901 gatgacatgc tgattggcga cggcatcaaa actggcctta gtaaaatggg ttttagtgtc
    30961 gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc ttatgatgcg
    31021 gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg cgaatggcga
    31081 gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc gtgatgcgtt agcggaacgt
    31141 gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc gttgatagaa
    31201 gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag caacgagctg
    31261 cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc tggcgaaccc
    31321 ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa cgctggtcgg
    31381 gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga ggtcaccagt
    31441 aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga ttttattcgt
    31501 accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa cgtcttagtc
    31561 tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg ctgctttcca
    31621 gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac acccaactga
    31681 tgctgtttgc caagcggtta agtacgctcg atctcaacga aatcaacgcg gcggatcgca
    31741 tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg ctgacctttg
    31801 ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga gaagatattc
    31861 cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa gacgatcctt
    31921 ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt ggccaggaat
    31981 gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc ccgtggctgg
    32041 tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa ctcgcgccgc
    32101 tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa ccactaaacg
    32161 cgactggcgt acccagcgaa gtgcgaccac tggttgagtc gctaaatcaa ctgttcgccc
    32221 gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct cacgaactac
    32281 gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct gacgatgatc
    32341 cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc gctactcgtc
    32401 tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt caggacgtcg
    32461 cggagatccc gcttgaagat ctcctgcaat cgtcggtgat ggatatttac cacacggcgc
    32521 agcaggcgaa aattgacgtg cgactgacac tcaatgccca cagcatcaaa cgcactggac
    32581 aaccgctatt gctaagtttg ttggtgcgaa accttctgga taacgcagtg cgctacagtc
    32641 cacagggcag cgtggtggac gtcacgctga atgccgataa tttcatcgtg agggataacg
    32701 gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat cgcccccccg
    32761 gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc gccaaattgc
    32821 atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg atttgaggcg aaggtgagct
    32881 ggtaagggta agacttttgc taaattcgca aaagactttg cacattttgc taattttacc
    32941 gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgaggtta aaaaatgagc
    33001 aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac
    33061 accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt tcgcatcgtt
    33121 cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg
    33181 gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa aaaatacatt
    33241 gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca gcgatggccg tacccgcaaa
    33301 gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata tatgctttct
    33361 ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcaattctt ccacggcgtt
    33421 ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg catggaaccg
    33481 ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg ctatactgaa
    33541 gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct ttagaaggag
    33601 ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag
    33661 gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac
    33721 ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat
    33781 tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca tctgcaaacc
    33841 ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat gaatattcgt
    33901 attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac cgggcaatgt
    33961 tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca gttgcggcga
    34021 tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg ctgatcgtct
    34081 tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt ggtttcacgc
    34141 aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg cttgcctttc
    34201 ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc gtaataaacc
    34261 tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc
    34321 accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat gtgcagacca
    34381 tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct cagatcgtcg
    34441 ctgttaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga ggccagcact
    34501 tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat gatcgcctga
    34561 tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc cacgaggaac
    34621 agttcggtac ggttaaggtc ctgcgcggta caatcagcca gtttgccagg cagcgccggg
    34681 ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg ctgggcgctg
    34741 gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag ccacaggata
    34801 aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt gacgcgaaga gagacgctct
    34861 ttcgtctgcc cggcaaactg cggatcctgc attttcaccg acagaacata ggcgcagcga
    34921 tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg atattcacag
    34981 aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt accgccctgc
    35041 atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc cggcagccac
    35101 agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa cggtttttct
    35161 ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc gtcctgatag
    35221 caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac gccggggcac
    35281 agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg cgggctgtca
    35341 aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg
    35401 ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt ataaacctga
    35461 ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg cgttaaccac cgaaatcccc
    35521 acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc gcctgcatgc
    35581 agacggcaaa gaatcaattc aaccgccggg acaccctctt ccgggtgaat atccaccggc
    35641 atcccgcgcc catcgtcaat aacttctaac gactggtcag catgtaagat tacatccacg
    35701 cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc ttgccccaaa
    35761 tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg
    35821 gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt tagcaattcg
    35881 agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga aataatcttc
    35941 gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg aagcgtagta
    36001 cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca gccagatcaa
    36061 atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat ggcgtgactc
    36121 tagcacatat tgctgcccgg tgtatgggtt ctcgttctga ccgagatagt ccgtcagcag
    36181 ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac attgtgacaa
    36241 ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat cgccgccatg
    36301 ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg gcaactgcgg
    36361 aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca agctcgcttt
    36421 tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca tcagtagcct
    36481 tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc
    36541 gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc cagcgtaaag
    36601 ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg
    36661 ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga
    36721 aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg atcgagccaa
    36781 ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg ttctggcgca
    36841 tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg
    36901 ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa cacccgcttc
    36961 gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg
    37021 cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag
    37081 gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc
    37141 tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt tacccctaac
    37201 agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat cctgactctg
    37261 gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta ggtttcacga
    37321 tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa ccagtccgct
    37381 aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt aggataatca
    37441 taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac catcgcgtca
    37501 tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt
    37561 tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg atattgtgcg
    37621 tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc
    37681 aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc
    37741 ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg
    37801 atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc ctgttcccgg
    37861 cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat accgcttgcg
    37921 gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc ccccgggctt
    37981 gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc ttcaatcgct
    38041 tcgcgacggg caacatcttc cacactttca ccctcttcta tcatcccggc aaccatctcc
    38101 agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc aatcagcaca
    38161 acttcatcgc gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt
    38221 tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa tctataaaga
    38281 tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac atcgtttttg
    38341 ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc actaatagtg
    38401 aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt gttaaattga
    38461 ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga
    38521 atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa
    38581 tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt tcgttgagcc
    38641 aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta
    38701 agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac
    38761 tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg
    38821 gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt tttgatatgt
    38881 cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac gtcacatatc
    38941 agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg
    39001 ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt caattagatc
    39061 aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg
    39121 cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg
    39181 tagagcagct gcgccagatc accggtaact actatccgga actggcggcg ctgaatgtcg
    39241 aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac
    39301 gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag caaattcgcc
    39361 aggcgcagga tggtcactta ccgacgctgg atttaacggc ttctaccggg atttctgaca
    39421 cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac gacagcaata
    39481 tgggccagaa caaagtgggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta
    39541 actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa ctggaaagcg
    39601 cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat gcatctatca
    39661 gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta gacgcgatgg
    39721 aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgca accaccacgc
    39781 tgtacaacgc taagcaagag ctggcaaatg cgcgttataa ctacctgatt aaccagttga
    39841 atattaagtc agctctgggt acgttgaacg agcaggatct gctggcactg aacaatgcgc
    39901 tgagcaaacc ggtttccact aatccggaaa acgttgcacc gcaaacgccg gaacagaatg
    39961 ctattgctga tggttatgcg cctgatagcc cggcaccagt cgttcagcaa acatccgcac
    40021 gcactaccac cagtaacggt cataaccctt tccgtaactg acgacgacga cggggcttcg
    40081 gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt
    40141 ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga
    40201 agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg
    40261 cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gctggctgtg
    40321 aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc
    40381 caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta
    40441 ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc
    40501 agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc cagcaatcca
    40561 gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg atgggcggcg
    40621 gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccagccagc ccggcttacg
    40681 gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc cgcaccatga
    40741 ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac tactaccgtt acccgtggcg
    40801 gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt
    40861 ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg
    40921 gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg
    40981 gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg aagaagtcac
    41041 cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct
    41101 gatgaccaaa ttccgcattc caaaacgcac ctggagtttt gtgcgtcagt catggctgac
    41161 tcaccagcca tcgctttatt cgcgtcttga tctggcatgg gatggcactg gcgaacctaa
    41221 actgctggaa aataacgccg atacaccaac gtcactatac gaggcggcgt tctttcagtg
    41281 gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa
    41341 cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg cgtgaacagt atggcttcca
    41401 gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt
    41461 gcaggactgc gcgacggaag ctgaaatcgc taccgagttc ctctacattg acgatatcgg
    41521 gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca acctgttcaa
    41581 gctgtatcca tgggaattta tgttgcgtga gatgttctcc accaagctgg aggatgcagg
    41641 cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc ttctaccgct
    41701 actatgggag atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga
    41761 tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt
    41821 gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg
    41881 gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg
    41941 tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc gtgcgttgat
    42001 cacccaggat atgtctcggt tttatccaca tatttttatt gagtaatcaa tgatgccgga
    42061 tggcattcgc catccggtaa ttgttaacct atctgcaccg acagcatact caggctgccc
    42121 atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc acctaagaca
    42181 tacaacaacg gcagatagtg ctccggcgtt gggttcgata acgcgccacc ttcatggtca
    42241 aggtaattca ccagaggatg ctgctctacc gccccttgcc acgtcagatt cgctttcaca
    42301 tactcattaa acgacatcgc ccaaggatat ggtgaactat caccgtgcca cttcactgta
    42361 cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc tcgcagcgct
    42421 gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc gatactcaac
    42481 tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca ggaaccgtgg
    42541 tcaaagcccc aggcttcttt atccagcgtc accgggaccg acgctaacag ctcaaccaga
    42601 tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag cgcctgcggg
    42661 aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc tgttccacgg
    42721 gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt catccccaac
    42781 ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact accgtgacct
    42841 aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa aggtgtcatt
    42901 ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca atgatgatga
    42961 tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca
    43021 gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc gtttctcggc
    43081 gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt tgcggcgggg
    43141 atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc tgaaggaatg
    43201 tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt tggcctggac
    43261 cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca gccgttgcca
    43321 aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca taacttcccg
    43381 gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt tggcatcgca
    43441 ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc ggtttatgcg
    43501 gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact agcagaaatt
    43561 cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt ggtcatggcg
    43621 gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    43681 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    43741 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    43801 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    43861 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    43921 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    43981 agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa tatttaacaa
    44041 tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc
//
LOCUS       DAZOXR010000009        43713 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZOXR010000009 DAZOXR010000000
VERSION     DAZOXR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.009"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHUNN_000457"
     CDS             complement(<1..559)
                     /locus_tag="ACHUNN_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0574934.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUNN_000458"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUNN_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0574935.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUNN_000459"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUNN_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0574936.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUNN_000460"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUNN_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0574937.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHUNN_000461"
     CDS             complement(4001..6322)
                     /locus_tag="ACHUNN_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0574938.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUNN_000462"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUNN_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0574939.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUNN_000463"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUNN_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0574940.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUNN_000464"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUNN_000464"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0574941.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUNN_000465"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUNN_000465"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0574942.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHUNN_000466"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHUNN_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUNN_000467"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUNN_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0574943.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUNN_000468"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUNN_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0574944.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUNN_000469"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUNN_000469"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0574945.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUNN_000470"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUNN_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0574946.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUNN_000471"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUNN_000471"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0574947.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUNN_000472"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUNN_000472"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0574948.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUNN_000473"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUNN_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0574949.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUNN_000474"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUNN_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUNN_000475"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUNN_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0574950.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUNN_000476"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUNN_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR0574951.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUNN_000477"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUNN_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0574952.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUNN_000478"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUNN_000478"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0574953.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUNN_000479"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUNN_000479"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0574954.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHUNN_000480"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHUNN_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0574955.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHUNN_000481"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHUNN_000481"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0574956.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUNN_000482"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUNN_000482"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0574957.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHUNN_000483"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHUNN_000483"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0574958.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUNN_000484"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUNN_000484"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0574959.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUNN_000485"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUNN_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0574960.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHUNN_000486"
     CDS             32202..32564
                     /locus_tag="ACHUNN_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0574961.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHUNN_000487"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHUNN_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHUNN_000488"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHUNN_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHUNN_000489"
     CDS             35034..35912
                     /locus_tag="ACHUNN_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0574962.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHUNN_000490"
     CDS             35923..36276
                     /locus_tag="ACHUNN_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0574963.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHUNN_000491"
     CDS             36288..37592
                     /locus_tag="ACHUNN_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0574964.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHUNN_000492"
     CDS             37604..38689
                     /locus_tag="ACHUNN_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0574965.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUNN_000493"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUNN_000493"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0574966.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUNN_000494"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUNN_000494"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0574967.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUNN_000495"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUNN_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUNN_000496"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUNN_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0574968.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="ACHUNN_000497"
     CDS             complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="ACHUNN_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR0574969.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11126 c        11988 g        10442 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cattcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaactcg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccc
//
LOCUS       DAZOXR010000010        43269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZOXR010000010 DAZOXR010000000
VERSION     DAZOXR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.010"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            53..571
                     /locus_tag="ACHUNN_000498"
     CDS             53..571
                     /locus_tag="ACHUNN_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0574970.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            587..1126
                     /locus_tag="ACHUNN_000499"
     CDS             587..1126
                     /locus_tag="ACHUNN_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0574971.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHUNN_000500"
     CDS             complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHUNN_000500"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0574972.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHUNN_000501"
     CDS             complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHUNN_000501"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0574973.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4495..5865
                     /gene="xseA"
                     /locus_tag="ACHUNN_000502"
     CDS             4495..5865
                     /gene="xseA"
                     /locus_tag="ACHUNN_000502"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0574974.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5862..6077)
                     /locus_tag="ACHUNN_000503"
     CDS             complement(5862..6077)
                     /locus_tag="ACHUNN_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0574975.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHUNN_000504"
     CDS             complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHUNN_000504"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0574976.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHUNN_000505"
     CDS             complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHUNN_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0574977.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHUNN_000506"
     CDS             complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHUNN_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0574978.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHUNN_000507"
     CDS             complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHUNN_000507"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0574979.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHUNN_000508"
                     /gene_synonym="gcpE"
     CDS             complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHUNN_000508"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0574980.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHUNN_000509"
     CDS             complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHUNN_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0574981.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13391..14545)
                     /locus_tag="ACHUNN_000510"
     CDS             complement(13391..14545)
                     /locus_tag="ACHUNN_000510"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0574982.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHUNN_000511"
     CDS             complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHUNN_000511"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0574983.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHUNN_000512"
     CDS             complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHUNN_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0574984.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHUNN_000513"
     CDS             complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHUNN_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR0574985.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22756..23601
                     /gene="sseA"
                     /locus_tag="ACHUNN_000514"
     CDS             22756..23601
                     /gene="sseA"
                     /locus_tag="ACHUNN_000514"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR0574986.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23937..24062
                     /gene="timP"
                     /locus_tag="ACHUNN_000515"
     CDS             23937..24062
                     /gene="timP"
                     /locus_tag="ACHUNN_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0574987.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="ACHUNN_000516"
     CDS             complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="ACHUNN_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0574988.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="ACHUNN_000517"
     CDS             complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="ACHUNN_000517"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0574989.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26473..26673)
                     /gene="iscX"
                     /locus_tag="ACHUNN_000518"
     CDS             complement(26473..26673)
                     /gene="iscX"
                     /locus_tag="ACHUNN_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0574990.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26685..27020)
                     /gene="fdx"
                     /locus_tag="ACHUNN_000519"
     CDS             complement(26685..27020)
                     /gene="fdx"
                     /locus_tag="ACHUNN_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0574991.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27022..28872)
                     /gene="hscA"
                     /locus_tag="ACHUNN_000520"
     CDS             complement(27022..28872)
                     /gene="hscA"
                     /locus_tag="ACHUNN_000520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0574992.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28889..29404)
                     /gene="hscB"
                     /locus_tag="ACHUNN_000521"
     CDS             complement(28889..29404)
                     /gene="hscB"
                     /locus_tag="ACHUNN_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0574993.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29500..29823)
                     /gene="iscA"
                     /locus_tag="ACHUNN_000522"
     CDS             complement(29500..29823)
                     /gene="iscA"
                     /locus_tag="ACHUNN_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0574994.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29840..30226)
                     /gene="iscU"
                     /locus_tag="ACHUNN_000523"
     CDS             complement(29840..30226)
                     /gene="iscU"
                     /locus_tag="ACHUNN_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0574995.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30254..31468)
                     /gene="iscS"
                     /locus_tag="ACHUNN_000524"
     CDS             complement(30254..31468)
                     /gene="iscS"
                     /locus_tag="ACHUNN_000524"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0574996.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31709..32197)
                     /gene="iscR"
                     /locus_tag="ACHUNN_000525"
     CDS             complement(31709..32197)
                     /gene="iscR"
                     /locus_tag="ACHUNN_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0574997.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32498..33235)
                     /gene="trmJ"
                     /locus_tag="ACHUNN_000526"
     CDS             complement(32498..33235)
                     /gene="trmJ"
                     /locus_tag="ACHUNN_000526"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0574998.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33354..34157
                     /gene="suhB"
                     /locus_tag="ACHUNN_000527"
     CDS             33354..34157
                     /gene="suhB"
                     /locus_tag="ACHUNN_000527"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0574999.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34302..35156
                     /locus_tag="ACHUNN_000528"
     CDS             34302..35156
                     /locus_tag="ACHUNN_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0575000.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35347..36627
                     /gene="csiE"
                     /locus_tag="ACHUNN_000529"
     CDS             35347..36627
                     /gene="csiE"
                     /locus_tag="ACHUNN_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0575001.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36619..37758)
                     /locus_tag="ACHUNN_000530"
     CDS             complement(36619..37758)
                     /locus_tag="ACHUNN_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0575002.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37918..38808)
                     /gene="hcaR"
                     /locus_tag="ACHUNN_000531"
     CDS             complement(37918..38808)
                     /gene="hcaR"
                     /locus_tag="ACHUNN_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0575003.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38944..40305
                     /gene="hcaE"
                     /locus_tag="ACHUNN_000532"
     CDS             38944..40305
                     /gene="hcaE"
                     /locus_tag="ACHUNN_000532"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0575004.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40302..40820
                     /gene="hcaF"
                     /locus_tag="ACHUNN_000533"
     CDS             40302..40820
                     /gene="hcaF"
                     /locus_tag="ACHUNN_000533"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0575005.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40820..41140
                     /locus_tag="ACHUNN_000534"
     CDS             40820..41140
                     /locus_tag="ACHUNN_000534"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0575006.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41137..41949
                     /gene="hcaB"
                     /locus_tag="ACHUNN_000535"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41137..41949
                     /gene="hcaB"
                     /locus_tag="ACHUNN_000535"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0575007.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41959..43161
                     /gene="hcaD"
                     /locus_tag="ACHUNN_000536"
     CDS             41959..43161
                     /gene="hcaD"
                     /locus_tag="ACHUNN_000536"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0575008.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10266 a        11948 c        10859 g        10196 t
ORIGIN      
        1 cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa tagtgatgaa
       61 atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg caggatgcca
      121 gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga ataccaaaca
      181 agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg tagacaactc
      241 ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt
      301 tatcggccac aacgtcggtt ctggcagcaa ttccggaacg acggcagggg cagttggcgg
      361 cggagctgta ggcgcggcag cgggttctat ggtgaatgat aaaaccttag tggaaggtgt
      421 ttctttaaca tataaggaag gcaccaaagt gtatacctcc acccaggtgg gtaaagagtg
      481 ccagtttacg acaggtttag ccgttgttat taccacaacg tataacgaaa cgcgtattca
      541 gccaaatact aaatgtcctg aaaagagcta ataatcagga ggagtcatga agaaagtttt
      601 tctttgcgcc atcttagcct ccttaagcta tccggctatc gcctcatcat tgcaggatca
      661 actctctgct gtcgcagaag cggaacagca aggtaaaaat gaagagcaaa ggcagcatga
      721 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc
      781 ccaggccgcc gctaacaaaa gagcggcaac ggcagcggca aataagaaag ctcgtcagga
      841 taaactggac gccgaagcca ctgcggacaa aaaacgcgat caaagttatg aagatgagct
      901 acgcagctta gagattcaga aacaaaaact gacgctggcg aaagaagaag cccgcgttaa
      961 gcgagaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
     1021 gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag
     1081 cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga tgttagtaac
     1141 ttcaagccta tgattcttga agataaaaaa ccctctgtag taacagaggg ttttgttcat
     1201 tcatagtgca gggattaaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1261 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1321 aggaaatcgt acggcagacg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1381 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtacc
     1441 ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1501 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1561 agcacacgaa cgccaaggcc tggtcccggg aatgggtgac ggtacaacat gtcgtacggc
     1621 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1681 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1741 tgtgctttac cggttgcaga cgccgcagat tcgataacgt cagggtagat ggtgccctgc
     1801 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1861 cgaccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1921 cgatcttctg ccggaacgtg aacaatgttc agaccaaagt gatcgccaaa catatccaga
     1981 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     2041 ttaccgatag cgcggtgcag tagcattgcg gttacggagg aatccacgcc accagagagg
     2101 ccgaggatga ctttatcgtc gcctacctgc tcacggatgc gggctacagc atcgtcgata
     2161 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2221 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2281 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2341 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2401 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cactgtcgtt tacgacttca
     2461 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2521 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2581 tgcggcgcac gcgggctgtt ttcttcagta gtactttccg ggccgccgga aagaataatg
     2641 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2701 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtct
     2761 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2821 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2881 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2941 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     3001 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3061 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3121 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3181 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3241 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     3301 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3361 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3421 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     3481 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3541 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
     3601 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3661 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3721 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3781 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3841 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3901 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3961 taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
     4021 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4081 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4141 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4201 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4261 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4321 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4381 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4441 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4501 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4561 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4621 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4681 ttccgcaaca gcaaccgccg ggtgaccttc cgtccacagc atggacaaca agttttagtt
     4741 cgcgccaata ttacgctcta cgagccgcgc ggtgactacc agataatcgt tgagagtatg
     4801 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4861 gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4921 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4981 cgcgatcctt ctctaccggt gatcatctac cccaccgccg ttcagggcga tgacgcaccg
     5041 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     5101 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5161 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5221 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     5281 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5341 atggcgatgg attattatct cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga
     5401 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     5461 caaaagcgga tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     5521 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5581 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgtacagct tagcgccacg
     5641 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5701 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5761 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagtt
     5821 aaaaacatcc agccagtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5881 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccgcag
     5941 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataatt
     6001 tccccgcagg atgggcaacg cgcatggccg ttatcctgat caagcacatg ctgacattgt
     6061 ggacaatgca gttccatatg aattcctccg gtagccatcc ctctacattc atagagggaa
     6121 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6181 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6241 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6301 tcatcaagta gcgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     6361 caatcggcgg gttataacca ccagcgtggg catatttcag cttcacacga cgaccgcgta
     6421 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6481 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6541 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6601 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6661 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6721 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggtatct tcaattgcct
     6781 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6841 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6901 tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6961 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     7021 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7081 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7141 gcagcacacg ctccagcaga cttaatacgc cacgaccgtg agatgcggcg atcgggtaga
     7201 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7261 ttttgtttgc caccaggaag gtcggttttt cacgagagcg cagatgtttg gcaatcgctt
     7321 catctgccgg catcaagccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7381 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7441 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7501 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7561 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7621 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7681 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7741 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7801 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7861 cgacgttaat ccagtgcaga taaccttcac tgtcacctac caccaggttg ccattataca
     7921 gcaccggcga agtcagcagg cgatgcagca gatcgctttg tgtccacagc gtaacgccgc
     7981 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     8041 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8101 gatcaagcgc cgtcaggtta ccattatagg ccagagcgaa aacaacgccg ttaacaacga
     8161 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     8221 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8281 cgccccccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8341 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt
     8401 gtaactgacc gttactagtg tggattaaca ccagaccgtc gctgaccacc gggcgagaaa
     8461 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8521 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc acaccgccag
     8581 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     8641 tttctttgcc atcatccgca ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga
     8701 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8761 cggaagtgct ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8821 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8881 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8941 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     9001 tcccatgcac tacgcgcacc ttgcttatca cctttgctca acaatgcttt accacgcagg
     9061 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9121 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9181 ttttcatcac tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9241 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9301 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     9361 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9421 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9481 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     9541 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9601 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9661 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9721 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9781 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9841 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9901 caagacgttc gaggcccatc gcaaaaccga ctgccggtgt tgcacgaccg cccagttgtt
     9961 ccaccagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg aggctgttag
    10021 tcacccactc gaaaacggta cggttatagt aatccagacc acgcaccaga cgctggttta
    10081 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10141 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10201 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10261 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10321 cttccagcga accaatagag ttcagctcaa gagttacgtg ttcgaagata cccagcgcac
    10381 gccaccagcg ggcagtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10441 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10501 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10561 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10621 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10681 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10741 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10801 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10861 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcca tgcgggttca atcatacacg
    10921 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10981 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgct tccagctggt cgatcatatc
    11041 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc
    11101 gccggtgacg ccgagtgtag aaaccagcgc ctcacctggg ccattcacca cgcagccgat
    11161 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt
    11221 accgataaca tcaaattcct gacgcgaaca ggtcgggcag gcgatgaagt taatccctcg
    11281 cgaacggata cgcagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11341 ggccgccagc gatacgcgca gcgtgtcgcc gatgccttcc gacagcagca gacctaaacc
    11401 aatggcggat tttactgccc cgctgcgtgc accaccggct tcggtgatcc ccagatgcag
    11461 cggctgatcg atctgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11521 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatgacg
    11581 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11641 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11701 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11761 gacgccgtat tccgctactt tcagcgcaat gcgatagtcg aagtggatgt cagccaccag
    11821 cggcacgtta acccgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11881 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11941 ttcgacatct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    12001 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12061 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12121 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12181 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttatacgg tgcctggccg
    12241 gttaaattca aattaccgtc tttacgctgc atcccgctaa acaatttttt accggtagca
    12301 tcagtgacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12361 gccggcgtgg tcacgccagc ctgatcggtt ggcaacggcg cagcaccatc tggcgttgtt
    12421 gctgccgttg gtaccggggt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    12481 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    12541 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12601 gacgtattta acgggacact ctgcccctgc tcgctattac tgctcagttc tgccgaagat
    12661 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12721 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccatca
    12781 cgttttttgc ggcgtttacc gagggaaaaa ctctgcattg gcgcaacttt tgcagcccgt
    12841 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12901 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12961 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    13021 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13081 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13141 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13201 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13261 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13321 attgcaactt aatacttcat tcataaaaca ttacgccacg gtacataaag taaccgtggc
    13381 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    13441 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13501 caccacgagt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13561 ggtcgatacg gctgttcgag ctgcgtccat acggcgcacc cgggaacggg ttccacggga
    13621 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13681 tgccgtcgtt aacgtggtca agcatcacgt attcaatagt gactcggccc tgattggcgt
    13741 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13801 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg
    13861 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtagaaagcg
    13921 tgacgcgacg tttagacagg ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13981 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    14041 gccgctgacc ggtgactttt gctgcgccga cgattttcgc cgcccgccac acctggccga
    14101 taatttccga cacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14161 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga
    14221 tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg
    14281 atgaacgctg ttcttcaacc acttccggtg cgcggatctc cgccacctct ttcagtttgc
    14341 cgcgcaacac tttgttgatg tcggtcatct catcaaagtt gtcgcagcaa tagtgataca
    14401 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14461 gcatctgctg acggttgaga tccagcaggt tgatttttcc atctttcgtg gtgacgtttt
    14521 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14581 cctggagggt tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14641 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14701 ggtgcgcggg cacacttcgc cttcgccgaa gaaataagcg atttcgcgag cggcagattc
    14761 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14821 agtaccggcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14881 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14941 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgagcct gttcaacggt
    15001 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15061 attaccaatg acgttttttg ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat
    15121 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    15181 ggattataat gagcaacagg gccgttgact attgatgaag gtaacatttt tttaaaataa
    15241 aatgagtttt agcaacaatc agggacaaaa cagactattg catgacaaat ttcactgtcg
    15301 cgatttgccc cacgtcatcc atcaccagca attgataatc acctttatcc gtcaaatgca
    15361 gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt cagaaaccac cagcgttcac
    15421 ctgcccctcc acttgattgc aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa
    15481 taatcgcgcc atcgcgaacg cctgtcagct gcagagggag ctgggcatcg tgaccgtatg
    15541 gcgggcaaat tgtcgaggcc ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc
    15601 agggttccag cggtagcggc cagacattta ccatttcttg cttcgcctgc gggcagtcag
    15661 cggcgacgcg ctgccctttg tcatccagcc agatggggaa gcgaatgcca ttaatccctt
    15721 cctgctctga cagtaacagc gttggcggct ggctgccatc cagcagccag gttgccaggc
    15781 ggcggcgaca gttaccgtca ccttccggca aagactgtcc gcccggccag cagataacgc
    15841 cacgagtgac tgagttcggg cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga
    15901 tattattgac ctgattcaac aatggtacgg cactggcaaa gccaaactga ccaacaacgg
    15961 gcgtgccgtc aggtctgcca gtccagatcc caatgacata gcgagcgtta accccaatcg
    16021 cccaggcgtc acgatagcca tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg
    16081 gcaaggcact atccggcaag ggttgcgctt catcagccat aatccggcga atgatccacg
    16141 ccgcccccga cgacattaaa ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt
    16201 tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc cgccatatct tccagttttg
    16261 caccagcgcc gccgagaatg agtgaaagat tcggcgcagc gccgttgggc aaatataacg
    16321 gcaatccaac attgcgtaac tttgccgcaa accgtttcgg tccataggct tccagcacct
    16381 gcacagcagg taagttcagc gagcgcacca gcgcctcgct catgctgatc gggccatgaa
    16441 aaccgctatc aaagttacct ggtcgataat caccggtgcg ccgggggacg tcttgcagca
    16501 gtgatgccgg gtggatcaag ccttcatcca gcgccagacc ataaacaaac ggtttgagca
    16561 ccgatcctgg cgatcggatc gcattgacca tatcaacatg accaaagcgt gaatcatcgt
    16621 tgagatcaac cgatcccacc cagccgcgaa cacgcatatc ggtatgatca accacgatca
    16681 tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac
    16741 gtcgttgaag accggcatcc agcgtagtaa cgattttgtc gcttttgctt ttaccgagca
    16801 tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg gggtgccagc cagatgggtt
    16861 cttcccttga ctcttttacc tgctcacggg accacacacc ttgcgcagcc atccgttcga
    16921 gcactttatt acgcgcggct tcggcacgct ccggccaacg atccgggcga agacggctgg
    16981 gcgcttgcgg caaaaccgcc agcattgccg cctcggaata gcttaaattc gcaggcgact
    17041 ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc
    17101 ggttaagata caaggtcaga atttcacgct tagacagatg ccattccagt tgcaacgcgc
    17161 gccagagctg gcgaattttg ccgccaaatg ttttggggtg aggatcaagc agacgagcaa
    17221 cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc cgaagtgaga tcttgccatg
    17281 ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt ccagaaccag cgatcttcat
    17341 aattgatcag cgcttcaagg taacgtggag aaacatcttc gattgttacc ggataacgcc
    17401 agatgccgtc agcatcggcg aagcgccaga gcggcgtacc atcctgcgcc acgaccactc
    17461 gtgcgggatt gacttcatgc agcggtagag gccagagttt atctgctccc cacgctgcaa
    17521 gaaaaaggag aaatggcgca gctgccagcg ttatccagca gccgcgtttg gttaacaagc
    17581 gaggcattta cggtcggaca atcagcagat cttcagccgc gccggtcgcc cgccattggg
    17641 gaacatacat tgattccacc atcggttgcg gtacctgata cgttcccggc gtcaccgccc
    17701 gcgccagata caccagcgtt accggttggt attcatcaac ggcaaccgcc gccacaaagc
    17761 gatcgtcacg gaactcaatg tgcttaatac tcgcctgctg catctggttc agtaagttct
    17821 gcacttcgcc accactttgc tccaggctgg cgctaccgtt cgccagattc tggttttcca
    17881 gttccagacc cacaggcagc agatccacga ctaacgcatc cggcacgctg ttactggctt
    17941 ttacctgcaa ccacaccagc accagatcgc cgctacgtaa cgagtccagc gatttgctct
    18001 taccatcagt accaagaata tgacgctcga tttgcagcac attgttcgcc ggtaaaggtg
    18061 cggattgcgg ataaccgctg gcatccatac gcaaccataa cggctgatcg ccactgttgc
    18121 tcacctgcaa ggtgacaagt tgatcgctat tcagattgct gttttgcgct ttctcgcctg
    18181 tcagcggctc agctgagaaa gaggtttgcg cctgccattt acccggtaaa tcctgaatcg
    18241 tacgggcagc caggaacaac gcgttacttt cctgcgtcga tagccagcgt tcaccaaacg
    18301 cctgctgcga aagtgtgttc agcaaagtgt actgctcatc gggtagcagt ttattttctt
    18361 ccagcaagga gagcattaac gcgttgtcgc gcagtggact accgtaatca cccagccata
    18421 tccgctcatc actatttctc ggcgttttca gcgccagcgc aatcgcttct tcaccacgcg
    18481 tcgcatcacc catggttttc agcgcaatgc caagttgcag cagcggtaaa ccagaagcgg
    18541 catctgcacg atgctcccag atttcacgca gcgcacccag tggtgctttt tgctgacggg
    18601 ccagcaccag cgcagcgtaa gactgaacgg cgaatttact ggctttgaga ttatccgcgt
    18661 acgggatcga catcatgccc ggatcttgta aatagcgcag cagacgctca ttaccccggt
    18721 taatggcgtc tgtcggcacg ctgtaaccct gttcgcctgc gcggaccagg aaatccatca
    18781 cgtaagccgt cagccagtac tcttcgtcac cgtttttatc ccacagcgca aagccgccgt
    18841 tatcacgttg catttgcagc aaacgggaaa tgccgatatc gaccgatgca cggcgtttct
    18901 catcactgtc gcctttgatg cccaacgcct gcagttgggc tgcgttggta taaagtgacg
    18961 ggaacaggcc gctggcggtt tgctcaagac agccgtacgg atacgctttt aactctttga
    19021 tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa caattgccct tccagcgtaa
    19081 caggcgagaa gttttgcaat ccatccgccg gaatcgccca tgtctcacca ggctgtaacg
    19141 ccgtaccgta attaaccgtt tgtgccggga acgccggacg gacgccgatt ttccactgct
    19201 tatgctgatc ggcaacggtt tcacccggta acgctaaccc gctaatggtg gcctgaattt
    19261 ctccatcgcc ataacccggc aatgctcgca ccgggataaa cagcgtagta cgcacacctg
    19321 gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca
    19381 gggcaacgtt cagtttttgc ggtttatcgg taagattagt gatatccagc gtcagacgcg
    19441 aggtatcgcc actcgccata aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa
    19501 ctatcacttt gctttcgttg ctaccgaaat catctgccgt ccaggcttgc gccatgacgc
    19561 gcaattcacc gttaaaatcg ccaatcggca gtgtaaccga gccttcgccc tgttcgttga
    19621 gcgttaccga cagcgcctgc tgcgcgacaa tattgacgtg attgaccggc ggtttaccac
    19681 cacgtttcag ctcatcacca tcgccaccga aacgcagagc tgccagacgc ccctgacctt
    19741 caataacctg accgtaaata tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg
    19801 cctgccacgg atctggcgta acgtagtcag taatattcag cacaccacta tcaacggcag
    19861 acaccagcac attcacctgt ttaggcttct cgccattttt agtgctggct ttaattttca
    19921 cggttaatgg ttgattggga cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac
    19981 ggttttcatc accaagcggc agatgcaaca caccaaccgc gcgttttggc gtcgcggagc
    20041 gagatttatc gccaggacgc accaccagcg tactcaaata aagatcatga cgattccagg
    20101 ttttatcgac cggaatcgtc agatccagcc cttgagccgg aacatcaatc tcttgccacc
    20161 acagcggccc ttcactggac tcgaccatcg cataaccttt acccgccgtt ggcgcggcaa
    20221 tatgcaactt aatggtatcg ccggggcgat aactggcttt atctagtttc agcgtgacac
    20281 ggtcgggtcg cactgcgccg ctcccgtcgc tgttgtcctg ccagctatag ccagcccaaa
    20341 aacgcacgct actgaccgct tcattcggcg ctttgacttc cagacgataa gcgccccact
    20401 ctaccgggaa actgacctta ccggtttcat ccgcctgaag atccagagtt tgttcatttt
    20461 cgatcagatc tttttgatca aactgtgact gccagccttc atcttctgac cagttccagt
    20521 agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc cgacacggct tttttcacgc
    20581 cttgcgcatc gctataaacg atgtcaaaag cggcgttact gccttcatca acaatgggct
    20641 gtttcaccgt gctgtcagtg cgataatcgt aaaccgattt caaggcgaac tgcggacgga
    20701 gccccggcaa tgcatcggca ggccagatag cctgctcagc gcggcgcgtc accgggcgac
    20761 cgcccgattc cagcaaactg ccctggaaaa taacctgcaa tggggaatgc gtttccttcc
    20821 actggctttc tgtagaaact tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat
    20881 ccagcgtgcg ggaaaggttt tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg
    20941 acacagcctc acgcagtggg cgcaggaaaa gttgcccttg caaagtatta ccattagcag
    21001 gcgcaccgta caggtagtat cccaccacgg agaatttcac ttcatcattc ggcgtaagcg
    21061 gggttttctc accggtcaga ttcagcgcca tacgctctgg cataaaatct tcgacgtgga
    21121 aatcccacat ccggtactgg ttatcgcccg tgttggcgcg aatatgccac ataccggttg
    21181 ccgcattgct atcgagtggc caggtaaagt ggtagaggcc attctccggc tgactaacga
    21241 cgttcctgag tacctgcccg tcaggtttaa tcacgtctaa cttgatgggt tgatcgggca
    21301 atgctttacc gtctgcatcg cgcagtaaac cattgaggat taccgtttca cctgggcgat
    21361 aaagatcgcg tgggccaaac atgaaaaact gtttgctata gcctggcgcg ccagcaatgt
    21421 taaattctgc taagtccagc gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt
    21481 tacgtgccag cagtaatgcc gcatttttat cattttccag ctgcacatgc ccctgagcgt
    21541 cactggttgc ctgagtcaga gtctgtcctt tctcatttaa taaagagact tcaattcctt
    21601 gctgggccgc gccgttttcc agactttggg taaagatatc cagacaattg tgataacggt
    21661 gagctgaaac gccgatatca cttaacgtaa acagcgtcgc gggattactg taatcgtaac
    21721 gtccagcctg attcatcaca gccagataca cgcccgcctg ctgaagcggt ttgatatcgc
    21781 ccagcggcag caataatttt tcacgggtgt tacgcgcagg attgagatca aaccgtccgg
    21841 tgtagaccag atccgccatc tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc
    21901 ggtattccca ttggctaatg aatgctggca gagattctgg cttaacgcgg aagaagttaa
    21961 catcgacatt attaacgttg agcgccatta ccggcagccc ttcaacgact ttgccaggca
    22021 gcagcgaacc acggctggca aaaccgacgc tgggttggat gtcgcgggta gttatagttt
    22081 tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa
    22141 cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtag ctctttaaga ttatctgaca
    22201 gctcccaggc accatccact ttgccgcttt ttttatcgac gacatgaata acgcgtgaga
    22261 aatcctgatc cgggtcgaga gggatggaga acgtcagcac cagcgtggct gcaccatcaa
    22321 gttggacttc agagagatcc agcaatgtca gcaccttacc ggcactctgt tgggccagtt
    22381 tttgtctctc cggcacggag agttttgctg aactcgcgtt ttctgaagag gccgctttag
    22441 taacttcaga aggcgcatct tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc
    22501 ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct
    22561 gccacaatgg cccgttagca acgtcgaata attattatgc gtgagaattt acgttatgta
    22621 attcagtatc acgcccatac aatccgaatt ttagtgagaa ttgtggctca gacagccaaa
    22681 tcagcctctt tcgcttgtcg cttttcacta aacatccgac aatttagcct aaccccggca
    22741 aaaatggaga tgcctatgtc cacgacatgg tttgtaggag ccgactggct cgccgaacat
    22801 attgatgacc cggaaattca gattatcgat gcccgcatgg cgtcgcctgg acaggaagat
    22861 cgtaacgttg ctcaggagta tctgaatgga catattcccg gcgcagtgtt ttttgatatc
    22921 gaagcgcttt ctgatcacac ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc
    22981 gccgtggcga tgcgtgaatt aggcgttaac caggataagc acctgattgt ctatgacgaa
    23041 ggtaatcttt tctcagcccc acgagcatgg tggatgctgc gcacctttgg tgtagagaaa
    23101 gtgtcgattc tggggggtgg acttgcaggc tggcagcgcg atgatctgct gttagaagaa
    23161 ggtgcagtag agctgccgga aggagagttt aacgccgcgt ttaatcctga agccgtggtg
    23221 aaagtaaccg atgtattatt ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc
    23281 ccggctgcac gttttaacgc agaagttgat gaacctcgcc caggtttacg tcgcggacat
    23341 attcccggag cactgaatgt tccgtggacg gaactggtgc gcgaaggcga actaaaaacg
    23401 accgatgaac tggatgcgat attttttggt cgcggcgtca gctacgacaa accaattatc
    23461 gtcagctgcg gctctggtgt aacggcagcc gtggttttgt tagcactcgc gacgctggat
    23521 gtgccaaacg tgaaactgta cgacggcgca tggagtgaat ggggcgcgcg ggcagattta
    23581 ccggttgagc cagtgaaata agtattttac aggcaataaa aaaccgccga atttggcggt
    23641 tttttattgc tggtctggtt cgcggccttt ccagcaggtt gtattaccgt agtaatgcaa
    23701 gcgcgtctca gcggagacaa tactcgccag taactctctt tttgtcaagc aaaagagagt
    23761 tattattgtt ctgttagtgt attatccact gcggcccttt ccgccgtctc gcaagcgggc
    23821 gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa gcgcgtctca gcgcttgcta
    23881 tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga
    23941 agatacggtg cttctgtatc gtacttattg tttctggtgc gctgttttcc caagtaaata
    24001 ataaccggag tctctccggc gacaatttac tggtggttaa caaccttcag agcagcaagt
    24061 aagcccgaat gccgcccttt agggcggcat attttagatt atccggtttt gtttaaagtc
    24121 acgcaaaaaa ccaccccagc gacgttcata gaatggcaca atatgttcgg taataaagtg
    24181 gctaattcct ttttcccctt ttttcacctg acaaatatcg attggttcat cgccaggtaa
    24241 tgtatcggtc gctacacttc ccgtcgcctg aataatttct tcgatatcac catcggcttc
    24301 aatgccaata agtaaattag gctgtgcctc ttcgttctct ttaattgaac aaataaaagc
    24361 acgcttcacc ggcttaatgg ttttaaataa ggtggtgagt gaatcaatca tttgtgctgg
    24421 cggctctgcg acttccgata atatcagcga ttcaccgcct tccaggattt cctggctgct
    24481 cagcggattt ccctcttcac caatcaacaa actgatttca cgcggcataa attctttacc
    24541 ggtcggcagt ttggcattaa ggaagagcgt ttcgccaagg gtcatctcaa acaatgtgcg
    24601 aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc tgttgaagcg cttctaacga
    24661 ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc cagtgctgta aatcaagcgc
    24721 actatcttca accacagcct cgccctgcgc cgccgtacca ggcacccaga cggtggattc
    24781 cagtagagta cggaaaaagg ccgggcggtg cgccggttcg gttgctgctt tttccagcag
    24841 gtcttcaagt tcgttttttg tttcggacat aagaaccaca attcattcaa cgttcggggc
    24901 gcaaatgcct gatgcgctac gcttatcagg cctacaaggg attcgcaatt tgttgaattt
    24961 gcagaatttg taggccggat aaggcgttta cgccgcatcc ggcatattag tttacgccgt
    25021 taacagatta gctatcgtgc gcacaccaag tcccgtagcg cccgcagacc actgttcaac
    25081 cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc cagccttgct gatagttctc
    25141 aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct gccggatacg ccgcgcttcc
    25201 ggtattgttc agttcggcaa agttagacgg cagctggctg cggtggaatt ccgccagcgg
    25261 cagacgccag aatggttcgt tctcttgtga ggcactcgcc agcaaacgac cggcaagcgc
    25321 atcgtcaaaa ctgaacagcg cgtgataatc attacccagc gcagttttcg ccgccccggt
    25381 gagggtcgcc gcatcaatga tcatttccgg tttctgcgca ctggcatcaa tcagaccatc
    25441 ggcaagcacc agacgccctt cggcatcagt gttcatcact tcaacttttt taccgttgcg
    25501 ataggtgatg atatcgccca gcttgaacgc attgccgcta atcaggttat ccgcacagca
    25561 gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg gcaaatgcca gcgccccggt
    25621 aaccgttgcc gcgccgccca tgtccgactt catcgagtcc ataaacgcag tctgtttgat
    25681 gctgtagccg ccggagtcaa aagtgatacc tttacctacc aggcacgcgt acactggcgc
    25741 ttctttatcg ccagttgggt tgtaatccag cgccagcaat accggagaac gttctgaacc
    25801 gcgtccgacg gtgtgcagcc ccatataacc ttgctcacgc agatcttcgc ctttggtgat
    25861 ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga tcaacagcac gctgtgccag
    25921 ttgcgatggt cccaattctt ctgccggtgc gttgatggtg tcacgcaccc agtcgatgat
    25981 catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc agatccggcc actccacttt
    26041 acgcgtgcct ttcggggctt tgtaaccttg ccagaatgcc cagcagcgat ccgcatccca
    26101 gccttcaccg cttaactgaa catgcttgat gcccagaccg tcaatcttgc gcgccgcacg
    26161 ctggatcagc cccagatcgt ctgccccgtt caaatgcagg gtaatgccgt cattattaat
    26221 gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt
    26281 catcgcttct gtcattttag ttatccttct ttgtaaaagg gccgccagtt ggcagcccca
    26341 ggatttttcc gctaaatgat tcgcgttgca gaaaggcggc aagtgagtga agccccagga
    26401 gcatagataa ctatgtgact ggggtgaacg agcgcagcca acgcatctgc ggcgtgaagc
    26461 atgacgcgga aattattcgg cctcgtccag ccagactaac aaaatcgctt cgagaatttt
    26521 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
    26581 atcggtgaat cgaaccgttt tcggatcaag atcaggatac gcatcgtaca gtgcttcgcc
    26641 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
    26701 tggttgatag tgtaacgcgg gatttcgact actaaatctt cgtcggtaac acgtgcctgg
    26761 cagcttaaac ggctttcggg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
    26821 tctgagcttt ctggcagcga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
    26881 gcacaggatt tttcacaggc gtgttcaatc tcgataccgt tacgcagagc tgcgtcgaga
    26941 atggtttcac cgctattagc ttccagaaca gcgccatcag gacagagatc ctgatgaggc
    27001 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
    27061 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
    27121 cgcttgttcg atggcgtcaa cgtcatcgcc ctgcgccact tcactcaggt gagcggcagc
    27181 atcgtcaatg acctgacgtt ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
    27241 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca ttcgggcttt
    27301 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
    27361 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
    27421 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccacc
    27481 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
    27541 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
    27601 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
    27661 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
    27721 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
    27781 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
    27841 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
    27901 tacacccgcg tctttaagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
    27961 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
    28021 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gcggcatcca gcagttcacg
    28081 ctgaacgcgg ttatcgctac gatcaggaat gcccgcctgc tcgcgaatgt aatccgccag
    28141 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
    28201 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
    28261 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cggtcggttc
    28321 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
    28381 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
    28441 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
    28501 cagcagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
    28561 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
    28621 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
    28681 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
    28741 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
    28801 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
    28861 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
    28921 cggcactgct ccgcagttta tcgagaaaac gcagcttacg cacggtatcc gccgccgcgt
    28981 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
    29041 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
    29101 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
    29161 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
    29221 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
    29281 cctggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
    29341 ggctcagcgc ctgggtatcg agttgataac gggcaggcaa gccaaagaag gtgaagtaat
    29401 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
    29461 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
    29521 gcaaccacac tcatctttga cgttcgggtt ggtgaattta aacccttcgt tcaggccttc
    29581 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
    29641 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
    29701 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
    29761 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
    29821 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
    29881 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
    29941 cttcagcaat atcggtattt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
    30001 attcggtgac cagggagctg gaagcgatag cagaaccgca gccgtaagtt ttaaaacgcg
    30061 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcatcacg tcgccacagg
    30121 ccggtgcccc caccatgccg ctgccgacgt tctcgtcgtt gttgtcaaag gaacccacgt
    30181 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
    30241 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
    30301 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
    30361 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
    30421 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
    30481 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
    30541 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
    30601 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
    30661 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcctcg cccattccga
    30721 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
    30781 tttgcgcttc gatgcgtacg cgcggtttac gacgtacata cagcgcaccg atacctttcg
    30841 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
    30901 taggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
    30961 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
    31021 tggaaacaag gatggtgtca tcacgcatcg ctgcttcaag ttctttcaga tcgataatgc
    31081 cgttacgctg cggtgccagg taggtgactt caaaaccttc gcgctccagc tgacggcagg
    31141 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
    31201 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
    31261 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
    31321 cttcttcagc ctgccagccg aaacggtgag aacgggaggc cgggttacca aaggttccgt
    31381 ccatcgtcat aaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
    31441 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
    31501 aggaaccagg ctaccggctg gatgtacgac cgtgtttacg gagcgcgata cgccccggcc
    31561 tgattctaat atcttgttat ctttaaccta actgtttcgg gtttcagcaa gttgacaaac
    31621 ttacgaatct ccgggagctt acataagtaa gtgaccggag tgagtatgtg cagtcaacgc
    31681 agatgcaaca cgaaacagga cggttaaatt aagcgcgtaa cttaacgtcg atcgcgtctt
    31741 gtatgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct
    31801 ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca
    31861 aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcaaccg cctttaccct
    31921 gacaacgggt agcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc
    31981 tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac
    32041 catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt
    32101 cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg
    32161 cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc
    32221 ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac
    32281 attcccgagt aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca
    32341 gtgccatttt tttatcgtgg cgtttgtacc tgttgtcctt ccagaccatg ggcgggttga
    32401 gggcaattat tcgcattagc gctggcctct tcccctcacc ctaaccctct ccccgaaggg
    32461 gcgaggggac cgtccgtgca tttatctggc ttagccctca ggccttatta cctttattct
    32521 gctgctctat agaagccaga atcccgcgca ggatattcaa ttcctggctt tccgggcgcg
    32581 cacgggtaaa cagacggcgc aatttattca tcacctgccc cggatggttt tcacggataa
    32641 aaccggttgc cagcagcgtt tgttccagat gaccataaaa acgctccaga tcatcgacca
    32701 gcggatacgg cgtctcttca tgctcgacct gctcgccatt ttcctgagtt gccagccagg
    32761 ccatacgcac ttcataggca ataacctgaa ccgccattgc caagttcagc gaactgtatt
    32821 ccgggtttgc cgcaatcgcg acatgataat ggcatttctg caactcttca ttggtcaagc
    32881 cgacgcgctc acgaccaaac accagcgcca ccggggtatt tgccgcttca gcgacgcttt
    32941 tcagaccgca ttcgcgcggg tcgagcatcg gccacggcag cgtgcgggag cgtgcgctgg
    33001 tgcccaccac caggctacaa ccggctaacg cttcgtcgag cgtatcgacg atgtgagcat
    33061 taccaatcac atcgctggcc cctgctgcca gggcaatcgc ctgggagtcg ggtttcacca
    33121 gtggattaac cagccacaga ttggttaatc ccattgtttt catggcacgg gcaacagaac
    33181 ccatattgcc ggtgtgtgac gtctccacca gcacaattcg aatattttgc agcattgtct
    33241 ttcttcatct aaagattatt cacgcatctt atcataaaac gaagacagat gccgatctcg
    33301 ctgctatact ctgcgccgtt ttcccgttct ttaacatcca gtgagagaga ccgatgcatc
    33361 cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg taatttaatt gccaaaaact
    33421 atgaaacccc ggacgctgta gaagcgagcc agaaaggcag taacgatttc gtgaccaacg
    33481 tagataaagc tgccgaagcg gtgattatcg acacgattcg taaatcttac tcacagcaca
    33541 ccatcatcac cgaagaaagc ggtgaacttg aaggtactga tcaggatgtt caatgggtta
    33601 tcgatccact ggatggcact accaacttta tcaaacgtct gccgcacttc gcggtatcta
    33661 tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt atacgatcct atgcgtaacg
    33721 aactgttcac cgccactcgc ggtcagggcg cacagctgaa cggctaccgt ctgcgcggta
    33781 gcaccgctcg cgatctcgac ggtaccattc tggcgaccgg cttcccgttc aaagcaaaac
    33841 agtacgccac tacctacatc aacatcgtcg gcaaactgtt caacgaatgt gcagacttcc
    33901 gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc tgcgggtcgt gttgacggtt
    33961 tctttgaaat cggtctgcgt ccgtgggatt tcgcggcagg cgagctgctg gttcgtgaag
    34021 caggcggcat cgtcagcgac ttcaccggtg gtcataacta catgctgacc ggtaacatcg
    34081 ttgctggtaa cccgcgcgtt gtgaaagcca tgctggcgaa catgcgtgac gagttaagcg
    34141 acgctctgaa gcgttaatga ctcaggcggg tgatgtcact cacccgccct cgcctttcag
    34201 gcgctattct gaaatacttc ctcaccgctt tactttcttt cgtcactctc ccaccatctt
    34261 tccccgatta atggatgata aagaagtcac aaggaaaggt tatggcgctg ccagtgaaca
    34321 aacgcgttct caaaattctg tttattctct ttgttgttgc cttctgcgtt tatttagtgc
    34381 cgcgcgttgc catcaacttc ttctattatc ctgacgataa aatttacggt cccgacccct
    34441 ggtcggcgga atccgtcgaa tttacggcta aggacggtac tcgtctgcaa ggctggttta
    34501 ttccttcttc gacgggccct gctgacaatg ccatcgcaac catcattcat gctcacggca
    34561 atgccggaaa tatgtccgcc cactggccgc tggtcagttg gttacccgag cgtaatttca
    34621 acgtttttat gtttgattat cgcgggtttg gtaaatcaaa aggcacgccg tcccaggccg
    34681 gattgctgga cgatacgcaa agtgccatca atgtggtgcg ccatcgcagt gatgtaaacc
    34741 cacaacggct ggtgctgttc gggcagagta ttggcggggc gaatattctt gctgttattg
    34801 gtcaggatga tcgtgaaggc atacgtgcgg tgatcctcga ctccacattt gcctcttatg
    34861 caaccatcgc caatcagatg atccccggca gtggctactt acttgatgag agttacagcg
    34921 gcgaaaatta tatcgccagc gtcagcccga tcccgctttt actcattcac ggtaaagctg
    34981 atcacgttat cccatggcag cacagcgaaa agttgtatag cctggcaaaa gagacaaaac
    35041 ggttgatcct aatcccggat ggcgaacaca ttgatgcttt ttccgatcgt cacggcgatg
    35101 tttatcgcga acagatggtg gactttatcc ttagcgcgtt gaatccgcag aactaaccca
    35161 tgatcgctag cacgataatc attcacaaaa ccaccttaag acatgctaat ccactggtca
    35221 gaacagttta agatgagaaa aattctgtga cgcttgccaa catttctgat gattagcatt
    35281 cccttcgcca tttccctgag caaactttag ctattcttat caattatgct tatgggagat
    35341 ctacagatga tgcctacgct tgctccacca tctgtccttt cggctcccca gcgccgctgc
    35401 cagatcttgc tgacgctctt tcagccgggg ttgaccgcca ccatggcaac cttcagcgag
    35461 cttaatggtg tggatgatga tattgccagt cttgatatca gcgaaacagg acgggagatc
    35521 ctgcgctatc atcaactcac actgacgact ggttatgacg gtagctaccg ggttgaaggt
    35581 acagtgctta accaacgttt gtgtttattt cactggctac gacgtggttt ccgtctgtgt
    35641 ccgtcattta ttaccagcca attcaccccc gccctgaaga gtgaactgaa gcggcgcgga
    35701 attgcgcgta acttttacga cgataccaat ctacaagcgt tagtgaatct ctgctcccga
    35761 cggctgcaaa aacgctttga aacgcgcgat attcatttcc tgtgtctgta tctgcaatat
    35821 tgtttgctgc aacaccacgc tggaattacg cctcagttta atccgctcca acgtcgctgg
    35881 gcggaatcct gccttgaatt tcaggtagcg caggaaattg gacgccactg gcagcgtcgg
    35941 gcgctccagc ctgtaccgcc tgatgagcca ctgtttatgg cattactttt ttccatgttg
    36001 cgggttcccg atccattgcg tgatgcgcat cagcgagaca gacaattgcg tcagtctatc
    36061 aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc gtttttatga tgaacagggg
    36121 ttatgcgatc agctttatac ccacctcgcc caggcgttaa atcgcagttt gtttgccatc
    36181 ggtattgata ataccctgcc ggaagagttc gccagactgt acccacgcct ggtgcgcacc
    36241 acccgcgcgg cgctggccgg atttgaaagt gaatacggcg tccatctttc tgatgaggaa
    36301 agcggtctgg tcgcggtgat tttcggtgcc tggctaatgc aggaaaacga tctgcatgaa
    36361 aaacagatta ttctactgac cgggaatgat agcgagcgag aagcgcagat tgagcagcag
    36421 ttacgcgaac taacgttact gccgctcaac attaagcata tgtcggtaaa ggcatttttg
    36481 cagacaggcg ctccgcgtgg cgcggcactg attattgcgc cttataccat gccattaccg
    36541 ctcttttcac caccgctgat ctatacggac ctgacgctga caacacatca acaggagcag
    36601 atccgcaaaa tgctcgaatc agcatgaggg aacaactttc gggcgcaaaa acattgccgg
    36661 aagcgccacc agcgccatta cccagaacac gccgtggccc agatattgat acaggaaacc
    36721 ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg acggcagagt aaaccgcctg
    36781 taaacggatg acttcgctac cctggcgaac agcaatatag cgcatggcgg ccaggtggca
    36841 gaccgtgaag gtgccgcaat gcaaaatttg caccactatc aaccacggca acgccgtagt
    36901 tgctcccata atgccccagc gcactacgcc gcaaatcgcc gagatcaaca gcatatcgcg
    36961 agcactacaa cggcggaaaa gtttattact cagcgcaaag ataatgactt ccgccaccac
    37021 gcccagcgac cacaaatacc ccaccgccga ggccgagtag ccagctgcct gccagtaaat
    37081 ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat aaacaaacgc aggccagaaa
    37141 gcgccagttc tggcgaacca gcgccaacca cgcagaccaa ccggtgctct cctgctggcg
    37201 acttgcccct tgtggctgaa tcgtcggacg gatgagaaag ccgagcagca tggatgccac
    37261 gcccaacgtc aacagcgcga ggatcacccg ataatcaaac atagtgacca gtttgcccgt
    37321 cagcgccgag ccaatgacaa acgccaccga gccccacagt cgcactttgc cgtaatcaag
    37381 cgggaactgc ttttgccacg tattcgccag tgcatcggtc aacggtacca gcggtgagaa
    37441 aaagaggtta aagccaatca tcaccagcat cagccacgct acgtgcgccc ccgcccagaa
    37501 ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc aaggcggaaa tcaggcggga
    37561 aggatcgctg acgcggggcg cgatgagcaa actcccgaga aaacgggcaa ccagacctgc
    37621 ccccaataac aggccgatgg tttctggcgt taaaccaatc cctttaagcc agacgctcca
    37681 gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa tagccgagcg ccaaccagcg
    37741 cgtggattgc aaaaccatga gtccctcccg tcaggaggcg ttagtctggg gttaatcgaa
    37801 tgttggtgca agttgaaaaa taagaatatt cgagcctgct tcccgatgtc tgtaacgcca
    37861 gttcacaagc actaaaaaac tgcgatccgc gacgcatgtt gcaaaaataa tgcgtaatta
    37921 tgccgttacg cttgccaaac gttcctgcac aatggcgatg aaatcgcgta atgccggctt
    37981 catctcccct ttcttccacg ccatcagtaa agcaatggaa ggcacatttc cggcaatggg
    38041 acgaaaaaca acctgcccgg tattaaaatt attcatataa ccgggtatca aagtgacgcc
    38101 cagccccatg cccaccagat tcatggtcac cagaatattc gttgccacct ggacgatatt
    38161 tggctggctg ttttcttgcg caaaccacgc tttaacgatc ggcgcaagcg aaccggaata
    38221 cgccggatcg gtactgacga aattcacgcc atccagttgc gcggcggtga tctctttttc
    38281 atgtgctaaa gggtgatcaa ccggtaacac caccactaat ggttcgtcaa aaagctccag
    38341 ataatcaatc tccgggctat aaacaggatg gcgcattaag ccgacgtcga gttcaccacg
    38401 gcgaattttt tcctcctgtt gcgtggtgat taaactcacc agctcaatca aggtgtctgg
    38461 ctgtctgaga cgaaacattg gtaatacttt tggcaataaa ttcacttccg ccgatggcac
    38521 aaagccaatg gttaattgtc tgtcttcctg aacaattttc cgcgcccgta atttggcatt
    38581 ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa cattctcccg ccgccgtcaa
    38641 cgcgactttg cgcttatccc tcaccagtaa cggaacaccg acacagtttt caagatcgcg
    38701 gatctggctg cttaacgaag gctgtgaggt atgcagtttt tccgccgcac gggtaaagtt
    38761 cagtgcctgc gccactgcga cgaaatagcg taaatgccgt agttccatca ccttcccctt
    38821 gttatcgaaa aaacgtctca gctggtagaa attaactatt tcacatatta gcaaccaacc
    38881 aacaacatcc ttatgacaca aaaatagaag gtcaatacat cttatctttc aggattaaaa
    38941 aatatgacca caccctcaga tttaaacatt taccaactga ttgataccca aaatggtcgg
    39001 gtcactccgc gtatttatac cgacccagat atttaccaac tggagcttga acgtattttc
    39061 ggccgttgct ggttatttct cgcccacgaa agccagatcc caaaacctgg tgatttcttt
    39121 aacacctaca tgggggaaga tgcggttgtc gtagtgcgtc agaaagacgg cagcattaag
    39181 gcgtttctca accaatgccg ccaccgggcc atgcgtgtga gttatgcaga ttgcggcaac
    39241 actcgcgcct ttacctgtcc gtatcacggc tggtcttatg gcattaacgg cgagttgatc
    39301 gatgtaccgc tggaacctcg cgcctaccca caagggttgt gtaaatccca ctggggacta
    39361 aacgaagttc cttgtgtgga gagttataaa gggctgattt ttggcaactg ggataccagc
    39421 gcaccgggcc tgcgtgatta cctgggtgac attgcctggt atctggatgg catgctggat
    39481 cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa agtgggtgat caactgtaac
    39541 tggaaattcc cggcagagca gttcgccagt gaccagtatc atgctctgtt cagccatgct
    39601 tctgccgttc aggtattagg ggcgaaagat gatggcagcg ataagcgcct cggtgatgga
    39661 caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc tgcaatttgg tcaggacggt
    39721 cacggtagcg gtttcttctt tactgaaaaa ccggatgcta atgtctgggt cgatggcgca
    39781 gtttcaagct attaccgcga aacctatgcc gaagcagaac aacgtttagg tgaagttcgc
    39841 gccctgcgcc tggcgggtca taacaatatt ttccccacgc tttcatggct caacggcact
    39901 gccacgctcc gcgtctggca tccgcgcggc cctgatcaag ttgaagtgtg ggcgttctgt
    39961 attactgaca aagccgcctc cgatgaagtt aaagccgctt ttgaaaacag cgccactcgt
    40021 gcttttggtc ctgctggttt tctcgagcag gatgactcgg agaactggtg tgaaatccag
    40081 aaattgctta aaggccaccg cgcccgcaac agcaaactgt gtctggaaat ggggcttggt
    40141 caggaaaagc gccgcgacga cggcattcct ggcattacta actatatctt ttcagaaact
    40201 gccgctcgtg gaatgtacca acgctgggcc gatcttctga gtagcgaaag ctggcaggaa
    40261 gtgctcgata aaaccgccgc ttaccagcag gaggtgatga aatgagtgcg caagtttcac
    40321 tagagttaca tcaccgcatt agccagtttc tctttcacga agccagctta ctggacgact
    40381 ggaaatttcg tgactggctg gagcagctcg acaaagagat tcgttacacc atgcgcacca
    40441 cagttaacgc gcaaacacgc gaccgccgca aaggcgtcca gccaccgaca acctggattt
    40501 ttaatgacac caaagaccag ctggagcggc gaatcgcccg tctggaaacg ggcatggcct
    40561 gggcagaaga gccgccgtca cgcacccgtc acttaatcag caactgccag ataagcgaaa
    40621 ccgacatccc aaacgtattt gctgtgcggg taaattatct gctttatcgg gcacaaaaag
    40681 agcgcgatga aacattctat gttggaacgc gtttcgacaa agttcgccgt ctggaagatg
    40741 acaactggcg cttgctggaa cgggatatcg tcctggatca agcggtaatc acttcccata
    40801 acctgagtgt actgttctga tgaatcgaat ttatgcgtgt cccgttgcgg atgtgccgga
    40861 gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc ctgttcaacg ttggcggcga
    40921 gttttatgcc attaacgatc gttgcagcca tggtaatgcg tcgatgtcag aagggtatct
    40981 ggaagatgac gccacggtgg agtgcccgct acacgccgcc agtttttgcc tgaaaacggg
    41041 gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact tatccagtac acgttgaagg
    41101 tggtgacatt ttcatcgact taccggaggc gcagccatga gcgatctgca taacgagtcc
    41161 atttttatta ccggcggcgg atcgggatta gggctggcgc tggtcgagcg atttatcgaa
    41221 gaaggcgcgc aagttgccac gctggaactg tcggcggcaa aagtcgccag tctgcgtcag
    41281 cgatttggcg aacatattct ggcggtggaa ggtaacgtga cctgttatgc cgattatcaa
    41341 cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg attgttttat cggcaatgca
    41401 ggcatctggg atcacaatgc ctcactggtt aatactcccg cagagacgct cgaaaccggc
    41461 ttccacgagc tgtttaacgt caacgtactc ggttacctgc tgggcgcaaa agcctgcgct
    41521 ccggcgttaa tcgccagtga aggcagcatg attttcacac tgtcaaatgc cgccaggtat
    41581 cctggcggcg gtggcccgct gtacaccgcc agtaaacatg ccgcaaccgg acttattcgc
    41641 caactggctt atgaactggc accgaaagtg cgggtgaatg gcgtcggccc gtgtggtatg
    41701 gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg aaacctcgat aatgcagtct
    41761 ctgacgccgg agaaaattgc cgccatttta ccgctgcaat ttttcccgca accggcggat
    41821 tttacggggc cgtatgtgat gttggcatcg cggcgcaata atcgcgcatt aagcggtgtg
    41881 atgatcaacg ctgatgcggg tttagcgatt cgcggcattc gccacgtagc ggctgggctg
    41941 gatctttaag gaagcacgat gaaagaaaaa acgatcatta ttgtcggtgg cgggcaagcg
    42001 gcggcaatgg ctgcggcctc gctacgccag caagggttca ccggtgagct gcatctgttt
    42061 tccgatgagc gacatcttcc ttatgaacga cctccactct cgaaatccat gttgctggaa
    42121 gattccccgc agttacagca ggtgttaccc gctaactggt ggcaggaaaa caatgttcat
    42181 ctgcattccg gtgtaaccat caaaacgctg ggtcgcgaca cacgagagtt agtgttaacc
    42241 aacggcgaaa gctggcactg ggatcagctt tttatagcaa tcggcgcggc agctcgaccg
    42301 ctgccgttgc ttgatgcact gggagaacgc tgctttaccc tacgccatgc cggtgatgcc
    42361 gccagactgc gagaagttct gcagcccgaa cggtcagtcg tgattgtcgg tgccggaact
    42421 attggtctgg aactggctgc cagcgccacg cagcgcagat gtaaggtgac agtgattgaa
    42481 ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg tgcaacgcta tcttttacag
    42541 cgccaccagc aggctggtgt gcgcattctg ctcaataatg ccattgaaca tgtggtcgat
    42601 ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc ttcaggctga tgtggtgatt
    42661 tacggtattg gtatcagcgc caacgagcaa ctggctcgcg aggccaacct tgatactgcc
    42721 aatggcattg tcattgatga ggcttgccgc acctgcgatc ccgcgatctt tgccggtggc
    42781 gatgtggcaa tcactcgtct tgataatggt gcactacacc gctgcgaaag ctgggaaaac
    42841 gccaataacc aggcgcaaat tgccgctgcc gcaatgttag ggctaccgct accgctactg
    42901 ccgccgccgt ggttctggag cgatcagtac agtgataact tacagtttat tggcgatatg
    42961 cgtggcgatg actggctttg tcgtggcaac ccggaaactc agaaggcgat ttggtttaat
    43021 ctgcaaaacg gcgtgcttat cggtgcggtc acgctgaatc aggggcgtga gattcgccca
    43081 attcgcaaat ggatccagag cggcaaaacg tttgatgcga aactgctgat agatgagaac
    43141 atcgcgctta aatcactgta accaggataa ttagcgaata tctcaacgcc tggggcgtgg
    43201 cgaggtgcaa gagtgtgtat tacgtttaaa tcacattatc ttgcaaaggg attggttatg
    43261 aacacacta
//
LOCUS       DAZOXR010000011        39862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZOXR010000011 DAZOXR010000000
VERSION     DAZOXR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.011"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(136..811)
                     /locus_tag="ACHUNN_000537"
                     /pseudo
     CDS             complement(136..811)
                     /locus_tag="ACHUNN_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(956..1564)
                     /locus_tag="ACHUNN_000538"
     CDS             complement(956..1564)
                     /locus_tag="ACHUNN_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0575009.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1748..2065)
                     /gene="metJ"
                     /locus_tag="ACHUNN_000539"
     CDS             complement(1748..2065)
                     /gene="metJ"
                     /locus_tag="ACHUNN_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0575010.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2342..3502
                     /gene="metB"
                     /locus_tag="ACHUNN_000540"
     CDS             2342..3502
                     /gene="metB"
                     /locus_tag="ACHUNN_000540"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0575011.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3505..5937
                     /gene="metL"
                     /locus_tag="ACHUNN_000541"
     CDS             3505..5937
                     /gene="metL"
                     /locus_tag="ACHUNN_000541"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0575012.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6076..6210)
                     /locus_tag="ACHUNN_000542"
     CDS             complement(6076..6210)
                     /locus_tag="ACHUNN_000542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575013.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6286..7176
                     /gene="metF"
                     /locus_tag="ACHUNN_000543"
     CDS             6286..7176
                     /gene="metF"
                     /locus_tag="ACHUNN_000543"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0575014.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7505..9685
                     /gene="katG"
                     /locus_tag="ACHUNN_000544"
     CDS             7505..9685
                     /gene="katG"
                     /locus_tag="ACHUNN_000544"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0575015.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9779..10684
                     /gene="yijE"
                     /locus_tag="ACHUNN_000545"
     CDS             9779..10684
                     /gene="yijE"
                     /locus_tag="ACHUNN_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0575016.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10711..11328)
                     /locus_tag="ACHUNN_000546"
     CDS             complement(10711..11328)
                     /locus_tag="ACHUNN_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR0575017.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11602..12705)
                     /gene="gldA"
                     /locus_tag="ACHUNN_000547"
     CDS             complement(11602..12705)
                     /gene="gldA"
                     /locus_tag="ACHUNN_000547"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR0575018.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12716..13378)
                     /gene="fsa"
                     /locus_tag="ACHUNN_000548"
     CDS             complement(12716..13378)
                     /gene="fsa"
                     /locus_tag="ACHUNN_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0575019.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13390..15891)
                     /gene="ptsP"
                     /locus_tag="ACHUNN_000549"
     CDS             complement(13390..15891)
                     /gene="ptsP"
                     /locus_tag="ACHUNN_000549"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0575020.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16200..17279
                     /locus_tag="ACHUNN_000550"
     CDS             16200..17279
                     /locus_tag="ACHUNN_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0575021.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17294..17614
                     /locus_tag="ACHUNN_000551"
                     /pseudo
     CDS             17294..17614
                     /locus_tag="ACHUNN_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17665..19961
                     /locus_tag="ACHUNN_000552"
                     /pseudo
     CDS             17665..19961
                     /locus_tag="ACHUNN_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19927..20805
                     /locus_tag="ACHUNN_000553"
     CDS             19927..20805
                     /locus_tag="ACHUNN_000553"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0575022.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20807..21148
                     /locus_tag="ACHUNN_000554"
     CDS             20807..21148
                     /locus_tag="ACHUNN_000554"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0575023.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21135..21986)
                     /locus_tag="ACHUNN_000555"
     CDS             complement(21135..21986)
                     /locus_tag="ACHUNN_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0575024.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22201..23934)
                     /locus_tag="ACHUNN_000556"
     CDS             complement(22201..23934)
                     /locus_tag="ACHUNN_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0575025.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24117..26768)
                     /gene="ppc"
                     /locus_tag="ACHUNN_000557"
     CDS             complement(24117..26768)
                     /gene="ppc"
                     /locus_tag="ACHUNN_000557"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0575026.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27120..28271)
                     /gene="argE"
                     /locus_tag="ACHUNN_000558"
     CDS             complement(27120..28271)
                     /gene="argE"
                     /locus_tag="ACHUNN_000558"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0575027.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28425..29429
                     /gene="argC"
                     /locus_tag="ACHUNN_000559"
     CDS             28425..29429
                     /gene="argC"
                     /locus_tag="ACHUNN_000559"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0575028.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29437..30213
                     /gene="argB"
                     /locus_tag="ACHUNN_000560"
     CDS             29437..30213
                     /gene="argB"
                     /locus_tag="ACHUNN_000560"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0575029.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30274..31647
                     /gene="argH"
                     /locus_tag="ACHUNN_000561"
     CDS             30274..31647
                     /gene="argH"
                     /locus_tag="ACHUNN_000561"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0575030.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31914..32831
                     /gene="oxyR"
                     /locus_tag="ACHUNN_000562"
     CDS             31914..32831
                     /gene="oxyR"
                     /locus_tag="ACHUNN_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0575031.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32814..34214)
                     /gene="sthA"
                     /locus_tag="ACHUNN_000563"
     CDS             complement(32814..34214)
                     /gene="sthA"
                     /locus_tag="ACHUNN_000563"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0575032.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34535..35185
                     /gene="fabR"
                     /locus_tag="ACHUNN_000564"
     CDS             34535..35185
                     /gene="fabR"
                     /locus_tag="ACHUNN_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0575033.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35185..35544
                     /locus_tag="ACHUNN_000565"
     CDS             35185..35544
                     /locus_tag="ACHUNN_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0575034.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35584..36684)
                     /gene="trmA"
                     /locus_tag="ACHUNN_000566"
     CDS             complement(35584..36684)
                     /gene="trmA"
                     /locus_tag="ACHUNN_000566"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0575035.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36798..36988
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37053..38897
                     /gene="btuB"
                     /locus_tag="ACHUNN_000567"
     CDS             37053..38897
                     /gene="btuB"
                     /locus_tag="ACHUNN_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0575036.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38842..39699
                     /gene="murI"
                     /locus_tag="ACHUNN_000568"
     CDS             38842..39699
                     /gene="murI"
                     /locus_tag="ACHUNN_000568"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0575037.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9096 a        10025 c        10999 g         9742 t
ORIGIN      
        1 tgtaaaatat aatatatgtc atttgagaaa aaagagcaga atgaggtaaa aatctttggc
       61 tgtgtattca gtctggtaat catccaagga aagcttatca gaagggtgat gactccaatt
      121 agtagaacat atattttcga taaaagcacc gatgactttt tcatgaagtt ctacagaacg
      181 cgagaaacag atggttctgc gggccagacg tttgattcgg gttctcaggg tcagattgtt
      241 acgctcaatg tgctgagtaa aaatcttacc agtcagatgc ttctccttcg gtaactctct
      301 agcgtaacta ccccattcgt cagtggtcaa catgccgatg gtaaaagggt aagcagcgcc
      361 cgcaattcac gacaggtttc gtcagtccgg gggccaaaag tgtaggccag aacaccacca
      421 gttttcgtgt tgtacacgta ccagagccag tgctgtcgcg ctttactgcc gacaaagctc
      481 cattgctgac taagtttgca gatgagtgcc acatcagcat gagcgaccgg ggatgacgtt
      541 attcttcgtg gcgagagttt tttaaagttc ggatgactgt attaatgccg attttcagcg
      601 tacgggcagt atcgcgaacc ccggcaccgt tgaaagtcat ttcagcgatt tggtctttaa
      661 ctcccggttt acggggctcg taacagtaac tgagctgaaa tacccggtga cagtcacggc
      721 cgcggaatcg ctcatgtctt tttgggttct gcccgtgacg gtaaacctga acggactggc
      781 aacgggggca atgaaggtta actgtgacca tgaaacaacc ttaaaagcga gtattatatc
      841 cttaattcaa ctaattggag gcatcaccgt ttaccggagc gacagcttgc ggaaaggtcg
      901 tgcaactaca aagcaacaaa cagactacgg cgggaattat ttttttcatc atccattacc
      961 acgaaaacag cggccactct ttggcgactg tggtgagttg tggcgcgtcg aaaacggctt
     1021 gtggtgaaac taccgtttcc tcaagcggaa tatttttgcc gtagcgttct ttgagcttag
     1081 cctggaccag cgggttgctg agatcgaact ctttcaactt ctgctgctcg cccacgcgca
     1141 ttcccagcgc gttctggtaa accttccaca ccacttccga acagtactgg cgatcgtcgc
     1201 tccatgagaa gctgaaatca tacggtttac cgagataacg ttttgccgtt tgcgccagtt
     1261 tttgctgttg ttcaacactc agtccgcctt caacgcggcg aacaacgtat ttgccctttt
     1321 caccatgggc gatccactgc ttgagcgggg ggtatttcac cgggccgacc gcttcaaaaa
     1381 cgtagggctt tttgttgcgt atcaccagca taccggtgtg gctataatcg gaatgggtcg
     1441 ccagttggat cgctttactt tgcgatgagc gagagatctg aaagatgatg tcgccggttt
     1501 gtggctgcca ggcaaaggca ggtacagaaa ccagcaggct gaggatcagc agcctgtttt
     1561 tcatagttaa acgtccatgt ataaaaagcg gtgggtcgca gacaacgtgc tcgttgttta
     1621 tgccggatgc ggcgtgaacg ccttatccgg cctacaagtt cgtgcaaatt caataaattg
     1681 caatatgacg taggcctgat aagcgtagcg catcaggcga ttccactccg cgccgctctt
     1741 ttttgcttta gtattcccac gtctccgggt taatccccat ctcacgcatg atctcttttg
     1801 ccgcttccgg gatttcgtcg ctgcgctctt tacgcagatc ggcatcatcc ggcaaaggtt
     1861 gcccggtaaa ggcatgcaga aacgcttcgc acagcagctc gctgttggta gcgtgacgca
     1921 ggttgttcac ctgacgacgc gtgcgttcat cggtgaggat ttttaacacc ttaagaggaa
     1981 tggaaaccgt aatctttttg acttgttcac tcttcttgcc gtgctcagcg tatgggctga
     2041 tatattcgcc gctccattca gccatgagat acttaatcct cttcgtcaat aaattgagac
     2101 cagaccacac aatttatgtg ggtactgacc gtaaacccgc atagtttacc gtacaggcgt
     2161 taccgtgaca tcgtgtaatg cacctgtcgg cgtgataatg catataattt taacggctat
     2221 ttgggatttg ctcaatctat acgcaaagaa gtttagatgt ccagatgtat tgacgtccat
     2281 taacacaatg tttactctgg tgcctgacat ttcaccgaca aagcccagga aacttcatca
     2341 catgacgcgt aaacaggcca ccatcgcagt gcgtagcggg ttaaatgacg acgaacagta
     2401 tggttgcgtt gtccctccca tccatctttc cagcacctat aactttaccg gatttaatga
     2461 accgcgcgcg catgattact cgcgtcgcgg caacccaacg cgcgatgtgg ttcagcgtgc
     2521 gctggcagaa ctggaaggcg gtgctggcgc agtacttact aataccggca tgtccgcgat
     2581 tcacctggta acgaccgtct ttttgaaacc tggcgatctg ctggttgcgc cgcacgactg
     2641 ctacggcggt agctatcgcc tgttcgacag tctggcgaaa cgcggttgct atcgcgtgtt
     2701 gtttgttgat caaggcgatg aacaggcatt acgggcagcg ctggcagaaa aacccaaact
     2761 ggtactggta gaaagcccaa gtaatccatt gttacgcgtc gtggatattg cgaaaatctg
     2821 ccatctggca agggaagtcg gggcggtgag cgtggtggat aacaccttct taagcccggc
     2881 attacaaaat ccgctggcat taggtgccga tctggtgttg cattcatgca cgaaatatct
     2941 gaacggtcac tctgacgtgg tggctggcgt ggtgattgct aaagacccgg acgttgtcac
     3001 tgaacttgcc tggtgggcaa acaatattgg cgtgacgggc ggcgcgtttg acagctatct
     3061 gctgctacgc gggttgcgaa cgctggtgcc gcgtatggag ctggcgcagc gcaacgcgca
     3121 ggcgattgtg aaatatctgc aaacccagcc gttggtgaaa aaactgtatc acccgtcgct
     3181 gccggaaaat caggggcatg aaattgccgc gcgtcagcaa aaaggctttg gcgcaatgtt
     3241 gagttttgaa ctggatggcg atgagcagac gctgcgtcgt ttcctgagcg ggctgtcgtt
     3301 gtttacgctg gcggaatcat tagggggagt ggaaagttta atctctcacg ccgcaaccat
     3361 gacacatgca ggcatggcac cagaagcgcg tgctgccgcc gggatctccg agacgctgct
     3421 gcgtatctcc accggtattg aagatggcga agatttaatt gccgacctgg aaaatggctt
     3481 ccgggctgca aacaaggggt aaaaatgagt gtgattgcgc aggcaggggc gaaaggtcgt
     3541 cagctgcata aatttggtgg cagtagtctg gctgatgtga agtgttattt gcgtgtcgcg
     3601 ggcattatgg cggagtactc tcagcctgac gatatgatgg tggtttccgc cgccggtagc
     3661 accactaacc agttgattaa ctggttgaaa ctaagccaga ccgatcgtct ctctgcgcat
     3721 caggttcaac aaacgctgcg tcgctatcag tgcgatctga ttagcggtct gctacccgct
     3781 gaagaagccg atagcctcat tagcgctttt gtcagcgatc tggaacgcct ggcggcgctg
     3841 ctcgacagcg gtattaacga cgcagtgtat gcggaagtgg tgggccacgg ggaagtatgg
     3901 tcggcacgcc tgatgtctgc ggtacttaat caacaagggc tgccagcggc ctggcttgat
     3961 gcccgcgagt ttttacgcgc tgaacgcgcc gcacaaccgc aggttgatga agggctttct
     4021 tacccgttgc tgcaacagct actggtgcaa catccgggca aacgtctggt ggtgaccggg
     4081 tttatcagcc gtaacaacgc cggtgaaacg gtgctgctgg ggcgtaacgg ttccgactat
     4141 tccgcgacac aaatcggtgc gctggcgggt gtttctcgtg taaccatctg gagcgacgtc
     4201 gccggggtat acagtgccga cccgcgtaaa gtgaaagatg cctgtctgct gccgttgctg
     4261 cgtctggatg aagccagcga actggctcgt ctggcggctc ccgttcttca cgcccgtact
     4321 ttacagccgg tttctggcag cgaaatcgac ctgcaactgc gctgtagcta cacgccggat
     4381 caaggttcca cgcgcattga acgcgtgctg gcctccggta ctggtgcgcg tattgtcacc
     4441 agccacgatg atgtctgttt gattgagttt caggtgcccg ccagtcagga tttcaaactg
     4501 gcgcataaag agatcgacca aatcctgaaa cgcgcgcagg tacgcccgct ggcggttggc
     4561 gtacataacg atcgccagtt gctgcaattt tgctacacct cagaagtggc cgacagtgcg
     4621 ctgaaaatcc tcgacgaagc ggggttacct ggcgaactgc gcctgcgtca ggggctggcg
     4681 ctggtggcga tggtcggtgc aggcgtcacc cgtaacccgc tgcattgcca ccgcttctgg
     4741 cagcaactga aaggccagcc ggtcgaattt acctggcagt ccgatgacgg catcagcctg
     4801 gtggcagtac tgcgcaccgg cccgaccgaa agcctgattc aggggctgca tcagtccgtc
     4861 ttccgcgcag aaaaacgcat cggcctggta ttgttcggta agggcaatat cggttcccgt
     4921 tggctggaac tgttcgcccg tgagcagagc acgctttcgg cacgtaccga ctttgaattt
     4981 gtgctggcag gcgtggtgga cagccgccgc agtctgttga gctatgacgg gctggacgcc
     5041 agccgcgcgt tagccttctt caacgatgaa gcggttgagc aggatgaaga gtcgttgttc
     5101 ctgtggatgc gcgcccatcc gtatgatgat ttagtggtgc tggacgttac cgccagccag
     5161 cagcttgctg atcagtatct tgatttcgcc agccacggtt tccacgtcat cagcgccaac
     5221 aaactggcgg gagccagcga cagcaataaa tatcgccaga tccacgacgc cttcgaaaaa
     5281 accgggcgtc actggctgta caatgccacc gtcggtgcgg gcttaccgat caaccacacc
     5341 gtgcgcgatc tgatcgacag cggcgatact attttgtcga tcagcgggat cttctccggc
     5401 acgctctcct ggttgttcct gcaattcgac ggtagcgtgc cgtttaccga gctggtggat
     5461 caggcgtggc agcagggctt aaccgaacct gacccgcgtg acgatctctc tggcaaagac
     5521 gtgatgcgca agctggtgat tctggcgcgt gaagcaggtt acaacatcga accggatcag
     5581 gtacgcgtgg agtcgctggt gcctgcccat tgcgaaggcg gcagcatcga ccatttcttt
     5641 gaaaatggcg atgaacttaa cgagcagatg gtgcaacggc tggaagcggc ccgcgaaatg
     5701 gggctggtgc tgcgctacgt ggcgcgtttc gatgccaatg gcaaagcgcg tgtgggcgtg
     5761 gaagcggtgc gtgaagacca tccgctggca tcactgctgc cgtgcgataa cgtctttgcc
     5821 atcgaaagcc gctggtatcg cgataatccg ctggtgatcc gtgggcctgg cgctgggcgc
     5881 gacgtcaccg ccggggcgat tcagtcggat atcaaccggc tggcacagtt gttgtaattt
     5941 cagaaattta ataatgcccg gtactcatgt tttcgggttt atggtttcta atgaaatata
     6001 ttgaattatc ataggattag gccggattaa gcgtttacga cgaatccggc aagaagcaat
     6061 aagtacatgg ttagtttata tttgcagtcc ggtttgcttt gcataccgga ttttcttttt
     6121 cttaccatcc tgaagttttt tcatcttccc tgatttttcc tcaccatcat tggtcatttt
     6181 tcggttgacg cccttcggct tttccttcat ctttacatct ggacgtctaa acggatagat
     6241 gtgcacaaca caacatataa ctacaagcga ttgatgaggt aaggtatgag cttttttcac
     6301 gccagccagc gggatgccct gaatcagagc ctggcagaag tccaggggca gattaacgtt
     6361 tcgttcgagt ttttcccgcc gcgtaccagt gaaatggagc agaccctgtg gaactccatc
     6421 gatcgcctta gcagcctgaa accgaagttt gtatcggtga cctatggcgc gaactccggc
     6481 gagcgcgacc gtacgcacag cattattaaa ggcattaaag atcgcactgg tctggaagcg
     6541 gcaccgcatc ttacttgcat tgatgcgacg cccgacgagt tgcgcaccat tgcacgcgac
     6601 tactggaata acggtattcg tcatatcgtg gcgctgcgtg gcgatctgcc gccgggaagt
     6661 ggtaagccag aaatgtatgc ttctgacctg gtgacgctat taaaagaagt ggcagatttc
     6721 gatatctccg tggcggcgta tccggaagtt cacccggaag caaaaagcgc tcaggcggat
     6781 ttgcttaatc tgaaacgcaa agtcgatgcc ggagccaacc gcgcgattac tcagttcttc
     6841 ttcgatgtcg aaagctacct gcgttttcgt gaccgctgtg tatcggcggg cattgatgtg
     6901 gaaattattc cgggaatttt gccggtatct aactttaaac aggcgaagaa atttgccgat
     6961 atgaccaacg tgcgtattcc ggcgtggatg gcgcaaatgt tcgacggcct ggatgatgat
     7021 gccgaaaccc gcaaactggt gggcgcgaat attgccatgg atatggtgaa gattttaagc
     7081 cgtgaaggag tgaaagattt ccacttctat acgcttaacc gcgctgaaat gagttacgcg
     7141 atttgccata cgctgggtgt tcgacctggt ttataaatag tgtggctttt gtgaaaatca
     7201 cacagtgatc acaaatttta aacagagcac aaaatgctgc ctcgaaatga gggcgggaaa
     7261 ataaggttgt cagccttgtt ttctccctca ttacttgaag gatatgaagc taaaaccctt
     7321 ttttataaag catttgtccg aattcggaca taatcaaaaa agcttaatta cgatcaattt
     7381 gacctacatc tctttaacca acaatatgta agatctcaac tatcgcatcc gtggattaat
     7441 tcaattataa cttctctcta acgctgtgta tcgtaacggt aacactgtag aggggagcac
     7501 attgatgagc acgtcagacg atatccataa cacgacagcc actggcaaat gcccgttcca
     7561 tcagggcggt cacgaccaga gcgcgggggc gggcacaacc actcgcgact ggtggccaaa
     7621 tcaactccgt gttgacctgt taaaccaaca ttctaatcgt tctaacccgc tgggtgagga
     7681 ctttgactac cgcaaagaat tcagcaaatt agattactac ggcctgaaaa aagatctgaa
     7741 agccctgctg acagaatctc agccgtggtg gccagccgac tggggcagct acgccggtct
     7801 gtttattcgt atggcctggc acggcgcagg tacttaccgt tcaatcgatg gacgcggcgg
     7861 cgcgggtcgt ggtcagcaac gttttgcacc gctgaactcc tggccggata acgtaagcct
     7921 cgataaagcg cgtcgcctgt tgtggccaat caaacagaaa tatggtcaga aaatctcctg
     7981 ggccgacctg tttatcctcg caggtaacgt ggcgctggaa aactccggct tccgtacctt
     8041 cggttttggt gccggtcgtg aagacgtctg ggaaccggat ctggatgtta actggggtga
     8101 tgaaaaagcc tggctgactc accgtcatcc ggaagcgctg gcgaaagcac cgctgggtgc
     8161 aaccgagatg ggcctgattt acgttaaccc ggaaggcccg gatcacagcg gcgaaccgct
     8221 ttctgcggca gcagctatcc gcgcgacctt cggcaacatg ggcatgaacg acgaagaaac
     8281 cgtggcgctg attgcgggtg gtcatacgct gggtaaaacc cacggtgccg gtccgacatc
     8341 aaacgtaggt cctgatccag aagctgcacc gattgaagaa caaggtttag gctgggcgag
     8401 cacttacggc agcggcgttg gcgcagatgc cattacctct ggtctggaag ttgtctggac
     8461 ccagacgccg acccagtgga gcaactattt cttcgaaaac ctgttcaaat atgagtgggt
     8521 acagacccgc agtccggctg gcgcaatcca gttcgaagcg gtagacgcac cggaaattat
     8581 cccggatcca ttcgatccgt cgaagaaacg taaaccgacg atgctggtga ccgacctgac
     8641 gctgcgtttt gatcctgagt tcgagaagat ctctcgtcgt ttcctcaacg atccgcaggc
     8701 gttcaacgaa gcctttgccc gtgcctggtt caaactgacg cacagggata tggggccgaa
     8761 atctcgctac atcgggccgg aagtgccgaa agaagatctg atctggcaag atccgctgcc
     8821 gcagccgatc tacaacccga ccgagcagga cattatcgat ctgaaattcg cgattgcgga
     8881 ttctggtctg tctgttagtg agcgggtatc ggtggcctgg gcatctgctt ctaccttccg
     8941 tggtggcgac aaacgcggtg gtgccaacgg tgcgcgtctg gcattaatgc cgcagcgcga
     9001 ctgggatgtg aacgccgcag ccgttcgtgc tctgcctgtt ctggagaaaa tccagaaaga
     9061 gtctggtaaa gcctcgctgg cggatatcat cgtgctggct ggtgtggttg gtgttgagaa
     9121 agccgcaagc gccgcaggtt tgagcattca tgtaccgttt gcgccgggcc gcgttgatgc
     9181 gcgtcaggat cagactgaca ttgagatgtt cgagctgctg gagccaattg ctgacggttt
     9241 ccgtaactat cgcgctcgtc tggatgtttc caccaccgag tcactgctga tcgacaaagc
     9301 ccagcaactg accctgaccg cgccggaaat gaccgcgctg gtgggcggga tgcgcgtact
     9361 gggtgccaac ttcgatggca gcaaaaacgg cgtcttcact gaccgcgttg gcgtattgag
     9421 caatgacttc ttcgtgaact tgctggatat gcgttacgag tggaaagcga ccgacgaatc
     9481 gaaagagctg tttgaaggcc gtgaccgcga aaccggcgaa gtgaaataca ctgccagccg
     9541 tgcggatctg gtatttggtt ctaactctgt cctgcgtgcg gtggctgaag tttacgccag
     9601 cagcgatgct cacgagaagt ttgttaaaga cttcgtggcg gcatgggtga aagtgatgaa
     9661 cctcgaccgt ttcgacctgc tgtaatctga ccttcttcag cgactgcctt tcaggcagtc
     9721 gctgaagttt ctttaccggc gtatagtgtc cacaggaaaa ctacacactg gatctctcat
     9781 gtctgccgca ggaaagagca acccactggc aatcagtggc ctggttgtgc tcacacttat
     9841 ctggagttat agctggattt tcatgaagca agtcaccagc tacatcggtg ccttcgactt
     9901 taccgcctta cgctgcattt tcggcgctct cgttttattc atcgtccttt tattacgtgg
     9961 tcgcggaatg cgcccgacac cgtttaaata caccttagcc attgccctgt tacaaacctg
    10021 cgggatggtt ggcctggcgc agtgggcgct ggtcagcggc ggagcgggga aggtggcgat
    10081 cctaagctat accatgccgt tctgggtggt gattttcgcc gcgttgtttc tcggtgaacg
    10141 cctgcgacgt gggcaatatt tcgcgatact gattgccgct ttcggcttat ttttggtgtt
    10201 gcagccgtgg caactcgatt tctcttcgat gaaaagtgcc atgctggcaa tcctctccgg
    10261 cgtcagttgg ggggcgagcg cgattgttgc taaacgtctg tatgcccgtc atccgcgcgt
    10321 ggatttattg tcgttaacat cctggcagat gctctatgcg gcgctggtga tgagtgtggt
    10381 cgctttactg gtgccgcaac gtgaaattga ctggcagccc accgtgttct gggcgctggc
    10441 ctacagtgcg attctggcga cggcactggc gtggagctta tggttgtttg tattgaaaaa
    10501 cttgcctgcc agtattgcca gcttaagcac actggccgtt cccgtttgcg gcgtactctt
    10561 ttcctggtgg ctgctcggcg agaatccggg ggccgttgaa ggtagcggta ttgtgctgat
    10621 tgtgatggca ctggcgctgg tgagccgtaa gaaaaaagaa gccgttagtg taaaaaggat
    10681 ctgaattttt tcttcatgtg gggcgatcgc ttatttaaca aaataacgat aatgccccac
    10741 catgcgccag ctaaacagca catcttcttc ctgcgcacct gcgccaatgt tatgtatcac
    10801 cagcggcgta ccgtcgcggg cgaagccatc tgaaaccacc ccaatatgtg ccagcccgtt
    10861 atctaatcgc caggagacaa tatcgcccgc ttgatagtca ctggggttct tgctgatggg
    10921 gcgtgtttta tcgtggtggg taaaccaggt ttccagatta ggcacccgac ggtgatcgat
    10981 gttgctgtcc gggcgcttta actgccattt ttgcgggtac tcggcaaaat tcttcgccat
    11041 atcttcgtgc accagtttct gcaaatcgac cttctggctg cgcaatgcac ggatcaccac
    11101 atcggaacat accccgcgtt tttgcgggac atcaccgcca ggataggtaa gctgcatata
    11161 cgccggatcg taaaatagcg tgctgccgat ttgctgtctg gcaccgtctg cgatggcaag
    11221 gttggtattg gcctggattt gtaccacggt tggtggaacg gcgggagatt ttaaggagtg
    11281 gctggtaaat gccgttagca ggctgagcag cgccagtgaa gctttcatct ggttcgtccg
    11341 tgaaaataag agtcacagat tcgaccttcc gggataacga ttgttctgta gagaaaatgt
    11401 ccagaagcgg ataaaaaact cctgaacaac gtgggagttt cagattactg gcacagagtg
    11461 ccggatgcgg cgtgaacgcc ttatctgtcc taaaaaaaaa gaaaattcaa tatattgcag
    11521 agattgcgta ggcctgataa gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac
    11581 aagccgggag tttggagtag gttattccca ctcttgcagg aaacgctgac cgtactggtc
    11641 ggctaccagc agagcggcgt aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat
    11701 ggtttcacat tctgcacatg ccgcttcagc cacaattcgc attttcgccg ggacatcttc
    11761 tttaatatcc agttgagcga gagttattgg caaacctacc gcatggctaa gtgcagctac
    11821 ggtttcgatt tcctcaaccg gtgcgttttc cagaaccagc tgcgtcagcg taccaaatgc
    11881 cactttttca ccgtgataat agtgatgcgc gtccgggata gcggtcaggc cgttatgcac
    11941 tgcgtgcgcc gcagccagac caccactttc aaaaccaaca ccgctcaaat aggtgttcgc
    12001 ttcaatcacg cgctccagcg ccggagtcac tacatgctgt tcggcagcaa gcatcgcttt
    12061 ttcgccttct tccagcaggg tgttgtagca cagttcagcc agtgccagcg cagcctgggt
    12121 gcacttgccg cccgccatgg tggtcgcgcc gctacgagag caggcacgcg cttcaaacca
    12181 ggttgccagc gcatcgccga tacccgccgc taacagacgt gcaggtgcgc cagcgacgat
    12241 tttggtgtcg acaatgacca tattcgggtt atttggcaac agcagatagc ggtcaaactc
    12301 accctcatcg gtgtagataa cagacaatgc gctgcacggt gcatcggtag aggcgatagt
    12361 cggtgcgatc gctaccggaa cacccatgaa atgtgccagt gctttggctg tatcgagggt
    12421 ttttccgcca ccgataccga gaattgcgcc acactgcgca gtctccgcga tgccacgcag
    12481 acggtcgatc tcattttgcg aacatccacc gccaaacggc gcaatttcta ctaccagtcc
    12541 agcatcttta aagcttttct cgacagtgga ttgagcaaaa cctaaaacaa atttgtcacc
    12601 caccactaac cagcgttctg ccagcggctt caggtattcg cccagacgat taatcacatc
    12661 agcgccctgg atgtatttac ccggtgattg aataatgcgg tccataattg ctcctttaga
    12721 gatgagtagt gccaaatgcg gcattccagt cgtgttcgaa cttctctata gctgactcta
    12781 ccgcaggggt gttgagcatt tgttgcgcta catctaaggg cagggtgatg gattcacatc
    12841 ctgccagtaa acagtccagc gcctgacgcg gcgttttaaa gctggctgcc agcaccatgc
    12901 tttctggcgc gtgcatttct aacagcgctt gcagctcctg aaccgtacga atgccgtctc
    12961 cgccctgggc atctacgcgg ttaacatacg gagcaacgta ttttgcccct gccagcgcgg
    13021 ccagtaaccc ttgtgcagca ctgtacactg cggtgccaag cgtagtaata ccctcttttt
    13081 tcagtatttt aattgctgcc agaccttcgg aagtcaccgg gattttcacc acaatacccg
    13141 gaatagcgtc gcgcaggcgc ttcgcttctt ccaccatccc ctgcgcgtcg cggctcatgg
    13201 tctgagcaaa cagaatgccc tcatcaccaa tcgctttttg cagacgcggc agcacttccc
    13261 atatggactc cttgctggca gcgataatgc tcgggttagt tgtcaccccg gcaatgggga
    13321 atatgcgtgc cagacgttcg acttctgcga cgttagcggt gtccagatac agttccatga
    13381 tgtttccttt tacagttcca gttcatgttg cagcaggctg gcgatagcgt cggcagaggc
    13441 ggcgttaacc agcgcgttac ggaattcttc gtgcatgatg cggcgagcga ggcgcgagaa
    13501 aatgcgcatg tgctgatcgc ccgcagcgtg tttgttcagg gttaacatga tgatgaattg
    13561 cgcttcatca tcaccccagc gcaccggcgc ttgcagacgc gcaacgctga tggtggattg
    13621 ctcaatgtgt tctgatttgc tgtgtggaat ggcaaaacta aagcccagac cggtagagaa
    13681 aacggcctcg cgcgcccaca agtcggcttc cagtttacgc ggatagcggc agcgccctgc
    13741 cagcagcagg ttatcggtca tgcctttgag cacttcttct ttgctgcgcc agtcgctttc
    13801 cagtgtgatg cactcggcgg tgaccagcgg tgcgtcttgt tgggtcatgc ggaattgcgc
    13861 cagcaggtgc tccacttcca gcgaagtacg gcaggccatt gcctggttga gcaacttgcg
    13921 gcactcacgg ctatcaagtt gcgccatccg cgctttcgcc gccggaattg atggtgcgct
    13981 catgctgagt tcatccagcc ctaagccgac cagcaacggc agcacggaac ctttcgctcc
    14041 cagctcaccg cacagaccaa tccatttacc ctggcgatgc accgcctgca cggcgtaatc
    14101 gagcgcccgc aagaatgccg gattcaggct gttgtagtga cgagtaacct tagcgttatc
    14161 gcgatccact gccagcagat attgcgtcag gtcattacta ccaatactaa agaaatcaat
    14221 ctcttcgcag cattgatcga tgatgaacat caccgacggc acttccagca taatgccgag
    14281 ctggatcttc tcatcaaacg gaatgtgttc gttacgtagt tgctgtttgg cttctgccag
    14341 tttttctttc acccataaga tctcttccat tgaggagatc atcgggatca tgattttcag
    14401 gctgccgtga gcggaggcgc gcaggatcga gcgtagttgt gtggtaaata acgacgcgta
    14461 ttcttcataa atacgcacgg cgcgataacc gaggaacggg tttgcctctg cgggaatgtt
    14521 tagataatca acgggtttgt cgccgccaat gtccatagtg cgcacaataa tgctgcgtcc
    14581 gttggcagac tccagcgcct gacaaaaaat gttgtacagc tcgctttcgc ccggtgcact
    14641 ggtgcgatcc atatagagca tttcagtgcg gaacaaacca acgccttccg caccattgcc
    14701 gaatgcggcc tgcgcttcca cggagtgagc gatgttagcg gcaatttcaa tgcggatacc
    14761 gtcagcggta cgggcttgtt gggtcagcca gacacgctgt tgctcacgca gggcgtcctg
    14821 tacgcgggct tcttgctgat aataacgagc tacggcttct cctggctcaa ccacaatcgc
    14881 cccggcgttg ccgtcgatat aaatcgtttg atgctgccac ggagtgaggg catcaatatc
    14941 cacaccaacc agcgtcggaa tgttgaacga acgggcaagg atcaccgtat gtgaggtggt
    15001 gccgccactt ttcagcaaca gacctttgag gtgattttta tccagttcga ggaactggct
    15061 gggggtcagt tcatcagcca tacaaatggc ggactgcgtc agtttgcccg gtgccgggaa
    15121 gcgttgctca ccgtagattt gctggagtaa ctggaagcag acgtcgcgta cgtccagggc
    15181 acgttcttgc aggtagctgc tgctggaacg ggcgaactct tcgcaaaagt gattcgcact
    15241 ggcgacaatc gcttcggcgc agcttaatcc ctcgctgacg cctgccagta agtgctgacg
    15301 taaagatgta tcgccagcca gggatcggtg agcttccagt atcgcgctgg tagcaccgtc
    15361 gctatccagc agacgaaact caatgttttt cagtaccagc gtcaggccgt tttccagtgt
    15421 ggattgctcg gcgtcaacgt ctttggctgc gggaagatta ctcagcgcat tgagatctaa
    15481 agaagagatc ggcgtcagaa tgccgcctgc actaccgctg cacacggtgc gggcgcggat
    15541 aatttgcgga ttcagattgg tcagtgaaac cggcagtggt tccagttcgt cagatttaac
    15601 ttccgccagc ggcgcatcgc agtgggggaa ttcatcgcgc agccattggc ttaaacgctg
    15661 gtgcgcttcc tgttcgtcgg ccccggaaat cagtaactgg cagttatcgc ccgccagcgt
    15721 atcggtgcca atcagcgcaa gggcgctttt ggcgttgccc ttgcggtcag tgtgcaggtt
    15781 atgccactca atttgtgatg aaaaagtatt acacagcgtt tcaacgtggc ttgccggacg
    15841 cgcatgtacg ccgttaggta gctcacaaat aaattccaca atcagggcca ttgcctctct
    15901 cccggtacgg ttttctgttt acaggataag agcgcacggc aacggcctgc catgtgacaa
    15961 atctgccaaa agctggacaa atgtaatgta accgtcaatt tgcgacgcgt ctcacaagac
    16021 gccgttttgc ggcatgcttc cggtttatcg caagttatga ggcggatcgc atttttgtac
    16081 tgatattaca aaagtccagt aaatggcctt tttatccact gtttgctccg cctgcgattg
    16141 cctattgttt cctccatcaa aaatatggac atgggctttc gcaagcccgg gagcagctta
    16201 tgaatgagtt ggtgcagatc ctgaaaaata cccgtcagca tttaatgacg ggcgtttcac
    16261 acatgattcc cttcgtggta tcgggcggta ttttgctggc ggtttccgtc atgttgtatg
    16321 gcaaaggcgc agtgccggat gccgtagccg atccaaatct gaaaaaactg tttgatatcg
    16381 gcgttgcggg cttgacgctg atggtgcctt tcctcgccgc ttacattggt tattccattg
    16441 cagaacgttc tgcgctggct ccatgcgcta tcggtacctg ggttggtaac agctttggtg
    16501 cgggcttctt tggtgcactg atcgccggga ttatcggcgg catcgtggtg cattacctga
    16561 agaaaattcc ggtgcataaa gttctgcgct cggtgatgcc tatcttcatt attcctatcg
    16621 tcggcacact gattaccgca ggcatcatga tgtggggctt gggcgagcct gtaggggcgt
    16681 tgaccaacag cctgactcag tggcttcagg ggatgcagca gggcagcatt gttatgctgg
    16741 cggtgatcat gggtctgatg ctggcgttcg atatgggcgg tccggttaac aaagtggcct
    16801 atgccttcat gctgatttgc gttgctcagg gtgtttatac cgtggtggcc atcgctgccg
    16861 tgggtatttg tatcccgccg ctggggatgg ggctggcgac gctgattggt cgtaaaaatt
    16921 tctccgcaga agagcgcgaa accggcaaag cggcactggt gatgggctgt gttggcgtta
    16981 ctggaggggc gattcctttc gccgctgccg atccgctgcg tgtcattcct tccatcatgg
    17041 tcggttcagt ttgtggtgca gtaactgcgg cgctggtcgg tgcgcagtgc tatgcaggct
    17101 ggggtggtct gattgtgctg ccggtggttg aaggcaagct gggttatatc gcagcagtgg
    17161 ctgtcggagc agtggtgacg gctgtttgtg tgaacgtgct gaaaagtctg gcgcgtaaaa
    17221 atgggtcttc gactgatgaa aaagaagacg acctggattt ggattttgaa attaattaat
    17281 tgaggaacga gccatgacga aaattattgc agtaaccgca tgtccttcag gtgttgccca
    17341 tacctatatg gcagcagagg cgctggaaag tgccgcgaaa gcgaaaggct gggaagtgaa
    17401 agtagaaacc taggggtcaa ttggtctgga aaatgaactg actgcggaag atgtggcgag
    17461 cgctgatatg gtgattctga ccaaagatat cggcatcaag tttgaagagc gttttgctgg
    17521 caagaccatc gtgcgcgtca acatcagcga tgcagtaaaa cgtgctgacg ccattatgag
    17581 caaaattgaa gctcacctgg cgcaaaccgc ttaagttctt ctcccgctcg caagggcggg
    17641 ttcgctttcc cacaggagtt cctcatgacg aatcgtatct ctcgcctcaa aactgcactg
    17701 tttgccaata cccgtgaaat ctcgctggag cgggcgctgc tttataccgc cagccatcgg
    17761 caaaccgaag gcgaaccggt gatattgcgc cgggcgaaag caacagcgta tatccttgaa
    17821 catgttgaaa tttcgattcg tgatgaagaa ctgattgccg gtaaccgcac cgtaaaaccg
    17881 cgcgccggga ttatgtcgcc ggaaatggac ccttactggc tgctgaaaga gctggatcaa
    17941 ttcccgacgc gtccgcagga ccgctttgct atcagcgaag aagataaacg tatctaccgt
    18001 gaagagttgt tcccgtactg ggaaaaacgt tcgatgaaag atttcatcaa cgggcagatg
    18061 acagatgaag taaaagccgc gaccaacacg cagattttca gcatcaacca gacggataaa
    18121 ggccaggggc acattattat tgattaccca cgcctgttga atcacggtct gggtgagctg
    18181 gtggcacaga tgcagcaaca ttgtcagcaa cagccggaga atcactttta tcaggccgcg
    18241 ttgttactgc tggaagcctc gcagaaacac attttgcgtt acgccgaact ggcggaaacg
    18301 atggcggcaa actgcacaga tgcacagcgt cgcgaagaac tgctgactat tgcggagatc
    18361 tctcgccata acgccgaaca taagccgcag acgttctggc aggcgtgcca gttattctgg
    18421 tacatgaaca tcattctgca atacgaatcc aacgccagtt cgctgtcttt agggcgcttc
    18481 gaccagtata tgttgccgtt ctatcaggca tctttaaccc agggcgaaga tccggcgttc
    18541 ctgaaagaac tgctcgaatc tttatgggtg aaatgcaacg acatcgtgct gttgcgctcc
    18601 accagcagcg cgcgttattt cgcaggtttc ccgaccggct ataccgcact gctcggcggg
    18661 ttaaccgaga acggacgtag cgcggtgaac gtgctttcgt tcctttgcct tgacgcctat
    18721 caaagcgtgc aattaccgca accgaacctc ggcgtgcgca ctaacgcctt gatcgacacg
    18781 ccgttcctga tgaaaaccgc cgaaaccatt cgcctcggca ccggtattcc gcaaatcttt
    18841 aacgatgaag tggtggtacc agcgttcctc aaccgtggcg tttcgctgga agatgcgcgc
    18901 gactattccg tagtgggctg tgtggaatta tctattcccg gcagaaccta cggcttgcat
    18961 gacatcgcga tgtttaatct gctgaaagtg atggaaatct gcctgcatga aaatgaaggc
    19021 aatgctgcgc tgacttatga aggtttactg gagcagatcc gcgccaagat cagccactac
    19081 atcaccctga tggttgaggg cagcaatatt tgtgatatcg gccatcgcga ctgggcacct
    19141 gtaccgctgc tctcgtcttt tatcagcgat tgtctggaaa aaggtcgcga tattaccgat
    19201 ggcggcgcgc gttataactt ctccggcgta caggggatcg gtatcgccaa cctgagcgat
    19261 tctctccatg cgttgaaagg gatggttttt gagcaacagc gtttaagttt tgacgaattg
    19321 ctgtcggtat taaaagccaa ctttgcaacg ccagaaggcg aaaaagtccg tgctcgctta
    19381 attaaccgct tcgagaaata cggtaacgat atcgacgagg tggataacat cagcgccgaa
    19441 ctgttacgcc actactgcaa agaagtggaa aaataccaga acccgcgcgc ggttacttca
    19501 cgccgggatc gtataccgtt tctgctcacg tcccgttggg atcggtggtt ggcgcgacgc
    19561 cagacggtcg ttttgccggg gaacaactgg cagacggcgg cttgtcacct atgctgggcc
    19621 aggacgcaca agggccaaca gcggtactga agtcagtcag taagctcgat aatacgctgc
    19681 tgtctaacgg tacgttgctg aacgtgaaat tcactccggc gaccctggaa ggtgaagcgg
    19741 gattacgcaa actggccgac ttcttacggg cgtttaccca gcttaagttg cagcatattc
    19801 agtttaacgt ggtgaacgcc gacacgttgc gggaagcgca acagcgccca caagattatg
    19861 ccgggctggt ggtgcgcgtt gccggataca gcgccttctt tgtcgaactg tcgaaggaga
    19921 tccaggatga catcatccgc cggacagcgc atcagctgta acgttgtgga aacgcgccgc
    19981 gatgatgtgg cgcgcatttt caacattcag cgttattcac tgaatgacgg cgagggcatt
    20041 cgtacggtgg tcttttttaa aggctgtccg catctttgcc cgtggtgtgc taatccggag
    20101 tcgatctccg gcaaaatcca gacggtacgc agagaggcga aatgtctgca ctgtgcgaaa
    20161 tgtttgcgtg atgcggatga atgcccctcc ggggcgtttg aacggattgg tcgcgatatc
    20221 agccttgacg ctctggaacg ggaagtgatg aaagatgaca ttttctttcg cacgtccggc
    20281 ggcggcgtca cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc tacccgtttt
    20341 ttacagcgac tgcggctgtg gggtgtgtca tgcgccattg aaactgccgg agacgcgcca
    20401 gcaagcaagc tgttaccgct ggcgaaattg tgcgatgaag tgctgttcga tttaaaaatt
    20461 atggacgcga ctcaggcgcg ggatgtggtg aagatgaacc tgccacgcgt gctggagaat
    20521 ctgcgtttgc tggtgagtga gggtgtcaac gtgatcccgc gtttaccgtt gatccctggt
    20581 ttcacgctca gcagggagaa tatgcagcag gcgctggatg tactgatccc gctgaaaatc
    20641 aggcagatcc atctgttacc gtttcatcag tacggcgaac cgaaataccg cctgctgggg
    20701 aaaacatggt cgatgaaaga ggtgcctgcg ccgtcgtcag ccgatgtgac aacgatgcgc
    20761 gaaatggcag aacgggccgg atttcaggtt accgtgggag gttaaaatgg catacctggt
    20821 ggcagtaacc gcctgcgtaa gcggcgtggc gcatacttat atggcggcgg aacggctgga
    20881 aaagttgtgc cagttagaga agtggggcgt cagcattgaa actcagggcg cgctgggaac
    20941 ggagaatcgt ttagcggacg aggatatccg tcgggcggat gttgctctgt tgattacgga
    21001 tatcgagctt gccggtgccg agcgatttga acattgccgc tatgtgcaat gcagcatcta
    21061 cgcattcctg cgtgagccgc agcgggtaat gagcgcggtg cgcaaagtgc tttctgcgcc
    21121 gcagcaaacc catcttattc tggagtagtc ggtttttctg tcagttggct gtggtactgc
    21181 cggcgatatt ccgacggcga gcgttcggtg tttttacgaa acagacggca gaagtagttg
    21241 ctgtcgacaa agccgcaggc gtgcgccact tcttttacct tcaggtcata gcctttcaat
    21301 aacgtcttag cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc
    21361 gttttttgaa acaggtgcga gagatagttt ggtgaaatat agaatgcctg tgcgacagat
    21421 tcgcgggtaa gcgcggaggc atagcgttcg tcgatataat cgcgaatagc ttcaaatagt
    21481 gcctggctgc gtgaggcggt ctggatttgg ctgccgagca aatcgcggca gtggctaagc
    21541 aggctggcta cgataagcct tgccgtttgc tgctcctgcg gctgcatttg catttcattg
    21601 agcgtttgta gcagaaaaga acctatacgt gggccgcgtc gggcgacgtg ttgcttcgcc
    21661 agattttgat attgtttgcc atcccattgc acgacgctga agccgagttg ctgtttgcca
    21721 aacagcacgc taaaggtggt agcgggtgtt ttccattgtg ggaaattcca gccaccagcg
    21781 gcaacgtaca gcacatcgcc gggaactaac gttgctccag cgccggtatc aacaaactca
    21841 ccttccagca caatttccag ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg
    21901 ccgttactgc tggcaaagta aatctggcgc agggacagcg ggccgttgat caggcgggag
    21961 agcagatagc tgacgccgtg atacatgtta ttcccttagg tatgtagacg ggaaaacagt
    22021 aactggcatt attaaaaaag gccacctgaa aagtgtggcc tgaggggagt tcgatgtaaa
    22081 cattgcggca gttgtattgc cggatgtggc gtaaacgcct tatccagcct accgccgcga
    22141 tctgtaggcc ggataagacg cgtcaagcgt cgcatccggc aaatagcgcc tggctgataa
    22201 ttactgatta cctacctggt cgccatacgg cagagtgtcg taatcgatca gcgcgttttt
    22261 cttgtacggg ttaccaatcc agcgggtagt ttctttgaac tgcggattca ccactgaacg
    22321 ggttggatcg taaccgtcgt aagataagcc cgccaaatct gaccaggtgt ggatcagttc
    22381 cgccaggctg tatttacgat cgacatcctg tgagaaatca cggggatgag tcgcttgcca
    22441 tttttccgac gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg ggttgtcttc
    22501 gttacgcccc tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt ggtcagagaa
    22561 gtaaaccagg aatccgttcg ggtctgccgc tttaaagtct ttaatcaggc tggcaaccac
    22621 atgatcgtta tacaggttag cgttgtcata atcgttatat gattccagct cttctgcgct
    22681 taatcctggc ggaacatgat cggtattgcc atcaaacttg ccctgatttt ccgggtagcg
    22741 gtatttgtat ttgatatgcg tacccagcag atgaacgatg atcagtttct tcggcgcagg
    22801 gtcattcagc acatcctgga acggcttcag cacgttggtg tcgtattcac gcgcactctg
    22861 cgtacgttgc tggttcatgt agtactgctt gtcggtctgg cgcgaaaata ccgtcagcat
    22921 ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag aaggttttat aacccgcctg
    22981 tttcatcatg ttcatcagcg acggctgcgt cagatacaga tccgggttct tttcattggc
    23041 aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa ctacgttatt
    23101 aaacacggtc agattcggat cggttttatg cagcgcatcc agctccggcg tggtttcacg
    23161 cggataaccg tacagactca tgcgtccgcg ctgggtcgac tcgccaatca ccagcactaa
    23221 agtgcgcggt tcgttacccg attcatcttt gaaattagcc agtggcggca aggcattatt
    23281 ttcattcagt aactttgtta gcgagtttag ttgctgacga tactgataat agccggtcag
    23341 gaattgccac ggtgcggcag gctccatacg cgaggccagg ttatccaacg ttttctcaaa
    23401 cggcttgttt ttgataaacg tattcatggc gatcggatgc agaatcaagc cataaagcag
    23461 ggcaaaagag acaacataac gccacggctt tggaatatag accgggcgca ggcgtgtcca
    23521 cagcagaact gccaccgccg tataggccag cgcgataagc acaattttca ggctgaaata
    23581 ctggcttaaa tactcgctgg cttcgttggt gttggtttcg aacatcacaa acagaacgct
    23641 ctgcgagaac tcctgcccgt agatgacgta gtagcatagc gccgccagag aggccgccca
    23701 tagcaccacg ccgattactg cggcaataat tttaatccgc ttcggaaaga ggaataccgg
    23761 gatcaaccac agcgagctga ataacagcga gtcgcgaatg ccgttggtgc cactataacc
    23821 actgatgtaa ataatggcct gtagcagagt agagaaaaac caaaagtaga gcagtgccca
    23881 acccagggct ttccagctaa aaaggggttt agcctggact tctgtggaat gcatagtaag
    23941 aacctgtctt gaaaaaaata tcgccgaatg taacgacaat tccttaagga tatctgaagg
    24001 tatattcaga atttgaataa aatgcagaca gaaatatatt gaaaacgagg gtgttagaac
    24061 agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga ggaagattag
    24121 ccggtattac gcatacctgc cgcaatcccg gcaatagtga ccattaacgc ttgttcgacg
    24181 cgaggatccg gttcctggcc ttctttttct gcctggcggg agcggtgcag caactcggcc
    24241 tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct gaatagactc tgcaatccac
    24301 ggcagatcgg ccatcagatg ggaatcgttg gcaatcgcca gcaccacttt gatgtcttct
    24361 tcttgcaggt tgcgtaactc tttacctaac ggccacagtg ctttgtctac cagacgttga
    24421 tcatagtatt ccgccaacca caggtctgct ttggcgaaga ccatctccag catgccgaga
    24481 cgcgtcgaga agaatggcca atcgcggcac atggcttcca gctcgctctg tttgccatct
    24541 tcgaccactt tttgcagcgc cgtacctgca cccagccagg cggggagcat caggcggttt
    24601 tgcgtccagg caaaaatcca cggtatggcg cgcagcgact cgacgccgcc ggttgggcga
    24661 cgtttcgccg gacgtgaacc caacggcagt ttgcccaatt cttgttccgg cgtagcggag
    24721 cggaagtaag gcacaaaatc tttgttttca cgtacgtagc cacggtagag atcgcaggag
    24781 atgactgaca gttcatccat aatgcgacgc cagctctctt tcggctcagg tggtggcagc
    24841 aggttggctt ccagaatcgc cccggtgtaa agcgacaggc tgctgacggt gatttctggc
    24901 agaccatatt taaagcggat catctcgccc tgttcagtca cgcgcaggcc acctttcagg
    24961 cttcctggcg gttgtgacag cagcgccgca tgggcaggtg cgccgccgcg accaatggaa
    25021 ccgccgcgac cgtggaacaa cgtcagctca atacccgctt tttcgcaggt tttgattaat
    25081 gcatcctgtg cctgatattg cgcccaggaa gctgccatca cgcccgcatc ttttgctgag
    25141 tcggaatagc caatcatcac catctgtttg ccctgaatca ggccgcgata ccagtcgata
    25201 ttgagcagct gggtcatgac atcgttggcg ttgttcaggt catcgagggt ttcaaacagc
    25261 ggagcaaccg gcatcgcaaa cccgataccc gcttctttca gcagcaggtg gacagccagt
    25321 acgtcggacg gcgttttcgc catcgagatc acgtaggcgg caatggagcc ttgcggcgct
    25381 tcggcaatca cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg ttgccagttg
    25441 cgcggcagaa gcggacgttt ggagttcagt tcgcggatca ggaacgcctg tttgtcggcc
    25501 tctgaccagc tttcgtagtc gccgataccg aggtagcggg tcagctcgcc cagcgcttcg
    25561 gtatggcggg tgctctcctg acggatatca atacggacca gcggtacgcc gaaacatttc
    25621 acgcggcgca gggtgtcgag cagatcgccg ttggcgataa tacccatgcc acacgcctga
    25681 agtgactggt agcaagcgta gagcggttcc cacagttctt cgttttgtgt cagcagccct
    25741 tctggttttg gcagttcttc gcctttcagg cgcgcttcca gccatgcctg tgtcgccatc
    25801 aggcgagaac gcaggttttt catcagatag cgatacggtt ctgcggcacc ttcttcgcca
    25861 accagcgcca gcagttcagg ggtcgcttca accatcgaca gttcagaaac cagaacctga
    25921 atatctttca ggaacagatc ggtggctttc cagcgactga gtagcaggac gtggcgggtg
    25981 atatcggcag tgacgttcgg gttgccgtcg cggtcaccgc ccatccacga agtaaaacgg
    26041 accggaacaa attcgacggg cagtttgtag ccgaggttct cttccagttg ttcgttcagt
    26101 tcgcgcaagt aattcggtac gccttgccac aggctgtttt ctactacggc aaagccccat
    26161 ttggcttcat ctaccgggct tggacgcagc ttacggattt catcagtatg ccatgactgg
    26221 gcgattaact ggcgcaggcg acgcatcagc tggttgtgtt cgtagtcagc gatatctttg
    26281 ttatcgagct gttttaagca ggcgttcact tccaccattt tgtggatcag tgtacgacgg
    26341 gtaatttcgg ttgggtgagc cgtgaggacc agttccagcg acagcgattc cactgctttt
    26401 ttgatggtgt cttcgctcag ttccggctgg tttttcagtt tgcgcagggt gcgggcgatc
    26461 acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg ctcggcggtg
    26521 ttggccaggt tcaggaactg actaaacgca cgcgcaacgg gcagcagctc gtcgttcgac
    26581 aaattttgta aggtggtgag caactcctgg cggttagcat cattgccagc gcgtgaagat
    26641 ttcgacaact tacggatagt ttctacgcgt tcaagaatgt gttctcccaa cgcatccttg
    26701 atggtttctc ccagcacttt gccgagcata ctgacattac tacgcaatgc ggaatattgt
    26761 tcgttcatat taccccagac accccatctt atcgtttgat agccctgtat ccttcacgtc
    26821 gcattggcgc gaatatgctc gggctttgct tttcgtcgtc ttttataaag ccacgtaaaa
    26881 gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga ataaatagca ggaatttacg
    26941 ccattaaatt cacgacgctt taaataagcg taacttatgg aaatgttaaa aaatcgccat
    27001 acataacacc aaaggtgtag gtcggataag atgcgcaagc atcgcatccg acattattgc
    27061 ggcaccggag tttggcaacg gtgccggatg cggcgcgagc gccttatccg gcctacgttt
    27121 taatgccagc aaaaatggtg aattacctgg ataatcaact cacgggtggg tttaataaac
    27181 cgcgtctcca gatattcatc aggctgatga gcttgattga tggaacccgg accgagcacc
    27241 agcgtcgggc ataacgtttg aataaacggc gcttcggtac agtaattcac cacttcggtt
    27301 tttgctccga gcaatttctc aaccacttca accagttgat gattcggtgg gcattcatag
    27361 ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg ttcgctcacc
    27421 ggagccaatg catcgttgag caaaccatta agttcattga gtgtcatgct aggcagcgga
    27481 cgaatatcca tatgcaactc acagcaagcg caaatacggt tagaagcgtc gccaccgtga
    27541 atatgcccga ggttgagcgt agggtatggc acggtaaatg cttcgtagtg ataacgttct
    27601 ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag ttcgatagcg
    27661 ttaactccgc gtgctggatc gctggagtgc cccgactggc cctgaatacg gatggcgtta
    27721 gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc aatgatggcg
    27781 caatccgggc gcagggcggt agtttcggca aaataacgcg ctccggccat actggtttct
    27841 tcatcagcag tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc gacatcgcgt
    27901 agcgtatcaa ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa gccgtaaagc
    27961 ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc atcaaatggc
    28021 accgtatcgg tatgccccgc cagcaacaag ccgccagccc cctgtccgat acttgccagc
    28081 atattgaatt tgttgcgagt tcctggcaca ggctgcactt ccacattgaa gcctaaatct
    28141 ttaaaccagt ccgccagcag agtgattaaa tctgcattgc tttgatcgag tgcctcttcc
    28201 gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa tctcgataaa tggcggtaat
    28261 ttgtttttca ttgttgacac acctctggtc atgatagtat caatattcat gcagtattta
    28321 tgaataaaaa tacactaacg ttgagcgtaa taaaacccac cagccgtaag gtgaatgttt
    28381 tacgtttaac ctggcaacca gacataagaa ggtgaatagc cccgatgttg aatacgctga
    28441 ttgtgggtgc cagcggctac gctggcgcag agctagtgac ctatgtaaat cgccatccgc
    28501 atatgaacat aaccgctttg actgtttcag cgcaaagcaa tgatgcggga aagttaatct
    28561 ccgatttgca tccgcagcta aaaggcatcg ttgagctgcc gttgcagccg atgtcggata
    28621 tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc caccgcccat gaagttagcc
    28681 acgatttagc gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt tccggtgcgt
    28741 ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg ctttacccat caatacccgg
    28801 aactgctgga acaggcagcc tacggtctgg cggagtggtg cggtaataaa ttaaaagaag
    28861 cgaatttgat tgcggtgcca ggctgttatc cgacggcggc acagctggcg ctgaaaccgt
    28921 tgattgatgc cgatcttctt gacctcaatc agtggccggt gatcaacgcc accagcggcg
    28981 tgagcggtgc aggacgtaag gcagcgattt caaatagttt ttgtgaagtt agcctgcaac
    29041 cgtatggcgt ctttactcat cgccatcaac cagagatcgc cacacacctc ggtgctgacg
    29101 ttatcttcac cccacatctg ggcaatttcc cgcgcggcat tctcgaaacc attacctgcc
    29161 gcctgaaatc gggtgtgacc caggcgcaag tcgcgcaagt gttacagcag gcgtatgccc
    29221 ataaaccgct ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat gtcgttgggc
    29281 tgccattttg cgatatcggg tttgccgttc agggcgagca tctgattatt gtggcgaccg
    29341 aagacaactt actgaaaggc gcggcggcac aagcggtaca gtgcgccaat attcgtttcg
    29401 gctatgcgga aacgcagtct cttatttaag ggtgcaatga tgaatccatt aattatcaaa
    29461 ctgggcggcg tactgctgga tagtgaagag gcgctggaac gtctgtttag cacactggtg
    29521 aattatcgtg agtcacatca gcgtccgctg gtgattgtgc acggcggcgg ttgcgtggtg
    29581 gatgagctga tgaaagggct gaatctgccg gtgaaaaaga aaaacggcct gcgggtgacg
    29641 cctgctgatc agatagacat tatcaccgga gcactggcgg gaacggcaaa taaaaccctg
    29701 ttggcatggg cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg tgacggcgac
    29761 agcgtcaaag tgacccagct tgatgaagag ttaggtcatg ttggactggc gcagccaggt
    29821 tcgcctaagc ttatcaactc cttgctggag aacggttatc tgccggtggt cagctccatt
    29881 ggcgtaacag acgaagggca actgatgaac gtcaatgccg accaggcggc aacggcgctg
    29941 gcggcaacgc tgggcgcgga tctgattttg ctctccgacg tcagcggcat tctcgacggc
    30001 aaagggcaac gcattgccga aatgaccgcc gcgaaagcag aacaactgat tgagcagggc
    30061 attattactg acggcatgat agtgaaagtg aacgcggcgc tggatgcggc ccgcacgctg
    30121 ggccgtccgg tagatatcgc ctcctggcgt catgcggagc agcttccggc actgtttaac
    30181 ggtatgccga tgggtacgcg gattttagct taagttttgt tggccggagg cgcagctttc
    30241 cggcattgaa tttcaaaata aggaaacaga gttatggcac tttggggcgg gcgttttacc
    30301 caggcagcag atcaacggtt caaacaattc aacgactcac tgcgctttga ttaccgtctg
    30361 gcggagcagg atattgttgg ctctgtggcc tggtccaaag ccctggtcac ggtaggcgtg
    30421 ttaaccgcag aagagcaggc gcaactggaa gaggcgctga gcgtgctgct ggaagatgtt
    30481 cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag atatccatag ctgggtggaa
    30541 ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg gcgtagtcgt
    30601 aacgatcagg tagcgactga cctgaaactg tggtgcaaag ataccgttag cgagttactg
    30661 acggctaacc gacagctgca atcggcgctg gtggaaaccg cacaaaacaa tcaggacgcg
    30721 gtcatgccag gttacactca cctgcaacgc gcccagccgg tgacgttcgc gcactggtgc
    30781 ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc gcttaagcgt
    30841 ctggatgtca gcccattagg ctgtggcgcg ctggcgggaa cggcctatga aatcgaccgt
    30901 gaacagttag caggctggct gggctttgct tcggcgaccc gtaacagtct ggacagcgtt
    30961 tctgaccgtg accatgtgtt ggaactgctg tcggctgccg ctatcggcat ggtgcatctg
    31021 tcgcgttttg ctgaagatct gattttcttt aacaccggcg aagcggggtt tgtggagctt
    31081 tctgaccgcg tgacttccgg ttcatcatta atgccgcaga agaaaaaccc ggatgcgctg
    31141 gagctgattc gcggtaaatg cggtcgggtg cagggtgcgt taaccggcat gatgatgacg
    31201 ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg aagacaaaga aggtctgttc
    31261 gacgcgctcg atacctggct ggactgcctg catatggcgg cgctggtgct ggacggcatt
    31321 caggtgaaac gtccacgttg ccaggaagcg gctcagcagg gttacgccaa cgccaccgaa
    31381 ctggcggatt acctggtggc gaaaggcgta cctttccgcg aggcgcacca tatcgtgggt
    31441 gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg aagatctgcc gctcgacgag
    31501 ttgcagaaat tcagcccggt gattggcgaa gatgtctatc cgattttgtc gctgcaatcg
    31561 tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc ccaagcgatt
    31621 gcttttgcgc aggcgcggtt ggggtaagaa catttatatg tacaaatttg agcctggctt
    31681 atcgccgggc ttttttatgg caaaaaaaag cggatcctgg agatccgcaa aagttcacgt
    31741 tggctttagt tattcgagtt gagaaactct cgaaacgggc agtgacttca agggttaaaa
    31801 gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa tgcttgatag ggataatcgt
    31861 tcattgctat tctacctatc gccatgaact atcgtggcga tggaggatgg ataatgaata
    31921 ttcgtgatct tgagtacctg gtggcattgg ctgaacaccg ccattttcgg cgtgcggcag
    31981 attcctgcca cgttagccag ccgacgctta gcgggcaaat tcgtaagctg gaagatgagc
    32041 tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc
    32101 tgctggtgga tcaggcgcgt accgtgctgc gtgaggtgaa agtccttaaa gagatggcaa
    32161 gccagcaggg cgagacgatg tccggaccgc tgcatattgg tttgattccc acggttggac
    32221 cgtacctgct accgcatatt atcccgatgc tgcaccagac ctttccaaag ctggaaatgt
    32281 atctgcatga agcacagacc caccagttac tggcgcaact ggacagtggc aaactcgatt
    32341 gcgtgatcct cgcgctggtg aaagagagcg aagcattcat tgaagtgccg ttgtttgatg
    32401 agccaatgtt gctggctatc tatgaagatc acccgtgggc gaaccgcgaa tgcgtaccga
    32461 tagccgatct ggcaggggaa aaactgctga tgctggaaga tggtcactgt ttgcgcgatc
    32521 aggcaatggg tttctgtttt gaagccgggg cggatgaaga tacacacttc cgcgcgacca
    32581 gcctggagac gctgcgcaac atggtggcgg caggtagcgg gatcacttta ctgccagcgc
    32641 tggctgtgcc gccggagcgc aaacgcgatg gggttgttta tctgccgtgc attaagccgg
    32701 aaccacgccg cactattggc ctggtttatc gtcctggctc accgctgcgc agccgctatg
    32761 agcagctggc agaggccatc cgagcaagaa tggatggcta tttcgataaa gtgttaaaac
    32821 aggcggttta aaccgtttaa cgcagctacc cgataggctt ccgccatggt cgggtagtta
    32881 aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac ctttctgttc cataatcgcc
    32941 tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct
    33001 tttgtttccc gatggaacaa aattttcagc gtgcccacgt tcatgccgac gatttgtgcg
    33061 cgtgccagat gtttaaactg ggcgcggccc acttcatatg gcactttcat cgcggtcagc
    33121 tgctgttcgg ttttgcccac agagctgatt tccgggatgg tgtaaatgcc ggtagggata
    33181 tcttcaatca gatgcgcggt ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc
    33241 tggtcatagg ccgctgatgc caggctcgga taaccaatca cgtcgcccac cgcgtaaacg
    33301 tgcggctgtg cggtctgata catgctgttg accttcagct gtccgcgact gtcagtttcc
    33361 agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc
    33421 aggcagtcag ctttcagttt tttacccgac ttcagatgca tgatcacacc gtcgtcacag
    33481 ccttcgatct tctcgtactc ttcgttgtga cgaatcacta cgccactgtt ccagaagtga
    33541 taggagagag aatctgacat ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg
    33601 atcagatcca cttttacatc cataccgcgg aagatcgacg catattcaca gccgatcact
    33661 ccagcaccat agataagtac atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg
    33721 tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat gatatggacg agagccgcag
    33781 gcaataacaa atttttcagc ggttagtgtt tcgacgctgc cgtccgggca atccagcgcc
    33841 aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata tttcacagtg attacgttcg
    33901 taaaatccct gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg gttaaggata
    33961 tcggcaaaag aagagcggag cagtcgcgaa tggtcgctgt atagtgggtt ttgattgaat
    34021 tctataatgc ggctgacggc gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg
    34081 caaccgccgc caacattttg ataacgctcg ataactgcga cgcgcacacc ttgcttaacc
    34141 aggcccattg cagcgccttc gccgccgggg ccggaaccta ttactatggc atcgtaatcg
    34201 taggaatgtg gcatggtagg gcttacctgt tcttatacat aaaagcaaca gaatggtaac
    34261 attctatcgc gggtaagcca attgatcccc gtcatttatc tggctatatc ctgagcggcc
    34321 tttgctttgt ctgtttctta cttttgccct gacgttttat tggattttta tcgacgatac
    34381 tctccgttta agcggcaggt ttccgctgta cgtaaaagaa ccggccagta aatatgtttt
    34441 attgcgttac cgttcattca caatactgga gcaatccagt atgttcattc tctggtatag
    34501 tgccagcagt acttttggca aggattcaga catcgtgatg ggcataagag cgcaacaaaa
    34561 agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag
    34621 cttcgccagc ctgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt
    34681 ttatcggcat ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt
    34741 aatgctacgc caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat
    34801 ccgcacctcg gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt
    34861 attattgcgg gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat
    34921 tcagcacttc attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc
    34981 gtttactgaa gcgcaagccg aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc
    35041 gttggacgtc ggcgtcgaac aacgtcggca attagaagag cgactggtac tgcaactgcg
    35101 aatgatttcg aaaggggctt attactggta tcgccgtgaa caagagaaaa ccgcaattat
    35161 tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg
    35221 ctggcgttag ttgctggctt atcgattaat ggtactttcg cagcgctgtt tagctccatt
    35281 gtgccatttt ctgtattccc gattatttcc ctggtgctga cggtttactg cctgcatcaa
    35341 cgttatctta atcgcactat gccggtaggc ttgccgggtc tggcagctgc ctgttttatt
    35401 ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac
    35461 ttcttcccgg cagtactctc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac
    35521 cgtaagcagg aagttgctga gtaatcggta ttatgccgga tgctggcgca tccggcatgg
    35581 gttttacttc gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc
    35641 aaacagagcc agacgttcga ccttgtgcgt ctggcttaat gtttccagat tcttgcataa
    35701 cgtttccggg ttacaggaga tgtacaaaat acacggatac gcctgcacca ttttctcggt
    35761 ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg gtttcgcact gataactctt
    35821 taagtcgatc ccttgcaggc ggttaaactc gcgcacacca ttcatcgcct gagtaaattc
    35881 ttctgccgcc atacgaataa tttgtacgtt atcaatatgg ttagctgcga tgttgtattg
    35941 cgcagcagca accgacggct tagcgatttc ggtggctaat acccgatcaa aattgcgcgc
    36001 cagcgctaat gaaaagttac cgttgccgca gtacagctcc agtaaatcgc cttttgagcc
    36061 tttggttacg tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggctgggt
    36121 aaagctgttt tctacctgac ggtagatcat ctctttccct gcgaccggca gacgctcgtc
    36181 gatgtaatct tgatccagct cgattttggt tttcgttgcc cgaccaatca gatgcacatt
    36241 cagattctgc gcgcgcagtg catcgcgcag ggcctccgct tcctgacgcc actcatcatc
    36301 cagcttctta tggtatagca gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc
    36361 aatctggaac aacttgtggc gcagaacggg attattacgc acacccgcaa tcatcgccgt
    36421 catcaactgg ttgataagtt cactggcggc ggggaagcta tccacgcgga tacggctttt
    36481 ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc
    36541 cgcacgcatc cggtaatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa
    36601 cggtgccatc atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc
    36661 tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt
    36721 agggattgct catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga
    36781 cagtaacttg ttacaacctg tagcatccac ttgtcggtcc tgtgagttaa tagggaatcc
    36841 agtgcgaatc tggagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg
    36901 acactgccat ccggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg
    36961 aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg
    37021 cggcatcctt cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgacgg
    37081 cgtgttctgt cacagccttt tccgcttggg cacaggatac cagcccggat actctcgtcg
    37141 ttactgctaa ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc accgttgtga
    37201 cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc
    37261 cgggcgtcga tatcacccaa aacggcggtt caggtcagct ctcatctatt tttattcgcg
    37321 gtacaaatgc cagtcatgtg ttggtgttaa ttgatggcgt acgcctgaat ctggcggggg
    37381 tgagtggttc tgccgacctt agccagttcc ctattgcgct tgtccagcgt gttgaatata
    37441 tccgtgggcc gcgctccgct gtttatggtt ccgatgcaat aggcggggtg gtgaatatca
    37501 tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc
    37561 agaactatga tgtctctacg cagcaacaac tgggggataa gacacgggga acgctgttgg
    37621 gcgattatgc ccatactcat ggttatgatg ttgttgccta tggtaataca ggaacgcaag
    37681 cgcagacaga taacgatggt tttttaagta aaacgcttta tggcgcgctg gagcataact
    37741 ttactgatgc ctggagcggc tttgtgcgcg gctatggcta tgataaccgt accaattatg
    37801 acgcgtatta ttctcccggt tcaccgttgc tcgatacccg taaactctat agccaaagtt
    37861 gggacgccgg gctgcgctat aacggcgaac tgattaaatc acaactcatt accagctata
    37921 gccatagcaa agattacaac tacgatcccc attatggtcg ttatgattcg tcggcgacgc
    37981 tcgatgagat gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta
    38041 gtattggtgc gggtgtcgac tggcagaaac agactacgac gccgggtaca ggttatgttg
    38101 aggatggata tgatcaacgt aataccggca tctatctgac cgggctgcaa caagtcggcg
    38161 attttacctt tgaaggcgca gcacgcagtg acgataactc acagtttggt cgtcatggaa
    38221 cctggcaaac cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg
    38281 ggacatctta taaggcacca aatctggggc aactgtatgg cttctacgga aatccgaatc
    38341 tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc gctggggtga
    38401 actggcgtat ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca
    38461 ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt
    38521 ttgataccgg accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga
    38581 ttaccgacac gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc
    38641 agttgtatga cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg
    38701 attactcatc ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg
    38761 cggttgcgta tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg
    38821 acaaagatta tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg
    38881 gcagctacac cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg
    38941 ttgtcggtct atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc
    39001 gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt ttattgttga gcgagtggtg
    39061 gcaattgtca ccgcggtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac
    39121 actgccagta ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt
    39181 gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg
    39241 gcaacccgcg gaacagttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat
    39301 gaatgccaga tagaaatgct gggctcggca gagatggttg agttggctga agcgaagcta
    39361 catggcgaag atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg
    39421 aaagagccgc cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa
    39481 ctgttacaag tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc
    39541 cgaacggcct ggttgttaga acatgaagcc ccggatgcaa aatctgccga tgcgaatatt
    39601 gccttttgta tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac
    39661 ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt
    39721 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    39781 actgacacgg aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaatctca
    39841 acagagaaaa gcaaaaaaat gc
//
LOCUS       DAZOXR010000012        39644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZOXR010000012 DAZOXR010000000
VERSION     DAZOXR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.012"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUNN_000569"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUNN_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0575038.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHUNN_000570"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHUNN_000570"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0575039.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUNN_000571"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUNN_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0575040.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUNN_000572"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUNN_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0575041.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUNN_000573"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUNN_000573"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0575042.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUNN_000574"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUNN_000574"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0575043.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHUNN_000575"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHUNN_000575"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0575044.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUNN_000576"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUNN_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0575045.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUNN_000577"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUNN_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0575046.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUNN_000578"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUNN_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR0575047.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUNN_000579"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUNN_000579"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0575048.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUNN_000580"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUNN_000580"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0575049.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUNN_000581"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUNN_000581"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0575050.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUNN_000582"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUNN_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0575051.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHUNN_000583"
     CDS             complement(14282..14581)
                     /locus_tag="ACHUNN_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0575052.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="ACHUNN_000584"
     CDS             complement(14793..15233)
                     /locus_tag="ACHUNN_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0575053.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="ACHUNN_000585"
     CDS             15334..15933
                     /locus_tag="ACHUNN_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0575054.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHUNN_000586"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHUNN_000586"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0575055.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHUNN_000587"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHUNN_000587"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0575056.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHUNN_000588"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHUNN_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0575057.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHUNN_000589"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHUNN_000589"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0575058.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHUNN_000590"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHUNN_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0575059.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="ACHUNN_000591"
     CDS             complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="ACHUNN_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0575060.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21877..22575
                     /gene="cpxR"
                     /locus_tag="ACHUNN_000592"
     CDS             21877..22575
                     /gene="cpxR"
                     /locus_tag="ACHUNN_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0575061.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22572..23945
                     /gene="cpxA"
                     /locus_tag="ACHUNN_000593"
     CDS             22572..23945
                     /gene="cpxA"
                     /locus_tag="ACHUNN_000593"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0575062.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="ACHUNN_000594"
     CDS             complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="ACHUNN_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0575063.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     PEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="ACHUNN_000595"
     CDS             complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="ACHUNN_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR0575064.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="ACHUNN_000596"
     CDS             complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="ACHUNN_000596"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0575065.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27022..28056
                     /gene="rhaT"
                     /locus_tag="ACHUNN_000597"
     CDS             27022..28056
                     /gene="rhaT"
                     /locus_tag="ACHUNN_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR0575066.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="ACHUNN_000598"
                     /pseudo
     CDS             complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="ACHUNN_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="ACHUNN_000599"
     CDS             complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="ACHUNN_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0575067.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30099..31568
                     /gene="rhaB"
                     /locus_tag="ACHUNN_000600"
     CDS             30099..31568
                     /gene="rhaB"
                     /locus_tag="ACHUNN_000600"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0575068.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31565..32824
                     /gene="rhaA"
                     /locus_tag="ACHUNN_000601"
     CDS             31565..32824
                     /gene="rhaA"
                     /locus_tag="ACHUNN_000601"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0575069.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33066..33890
                     /gene="rhaD"
                     /locus_tag="ACHUNN_000602"
     CDS             33066..33890
                     /gene="rhaD"
                     /locus_tag="ACHUNN_000602"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0575070.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33900..34214
                     /gene="rhaM"
                     /locus_tag="ACHUNN_000603"
     CDS             33900..34214
                     /gene="rhaM"
                     /locus_tag="ACHUNN_000603"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0575071.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34515..34961
                     /locus_tag="ACHUNN_000604"
     CDS             34515..34961
                     /locus_tag="ACHUNN_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0575072.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34972..36501
                     /locus_tag="ACHUNN_000605"
     CDS             34972..36501
                     /locus_tag="ACHUNN_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR0575073.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            36491..37561
                     /locus_tag="ACHUNN_000606"
     CDS             36491..37561
                     /locus_tag="ACHUNN_000606"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0575074.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37561..39309
                     /locus_tag="ACHUNN_000607"
     CDS             37561..39309
                     /locus_tag="ACHUNN_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR0575075.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39358..>39644)
                     /locus_tag="ACHUNN_000608"
     CDS             complement(39358..>39644)
                     /locus_tag="ACHUNN_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFR0575076.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9624 a        10300 c        10304 g         9416 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcgtgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacagcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgccca
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agctaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccacgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactccca ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg
    21181 tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg
    21241 agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca
    21301 actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca
    21361 ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg
    21421 ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc
    21481 gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca
    21541 taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc
    21601 tctgcgtact gcgctgcgta agttcttcac ccggatgcca gttatcgcct gaaccgactt
    21661 cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc catgacggca gcggtaacta
    21721 tgcgcatcat ttgctcccaa aatctttctg tcgcgattca acgatagaga gtttacgatt
    21781 caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc
    21841 tgatgacgta atttctgcct cggaggtatt taaacaatga ataaaatcct gttagttgat
    21901 gatgaccgag agctgacttc cctattaaag gagctgctcg agatggaagg cttcaacgtg
    21961 attgttgccc acgatgggga acaggcgctt gatcttctgg acgacagcat tgatttactt
    22021 ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc acttcgccag
    22081 acacaccaga cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc
    22141 ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg
    22201 gcacgtattc gcgcgatcct gcgccgttcg cactggagcg agcaacagca aaacaacgac
    22261 aacggttcac cgacactgga agttgatgcc ttagtgctga atccaggccg tcaggaagcc
    22321 agcttcgacg ggcaaacgct ggagttaacc ggtactgagt ttaccctgct ctatttgctg
    22381 gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa
    22441 cgcctgacgc ctttcgaccg cgctattgat atgcacattt ccaacctgcg tcgtaaactg
    22501 ccggatcgta aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg
    22561 gtttctgctt catgataggc agcttaaccg cgcgcatctt cgccatcttc tggctgacgc
    22621 tggcgctggt gttgatgttg gttttgatgt tacccaagct cgattcacgc cagatgaccg
    22681 agcttctgga tagcgaacag cgtcaggggc tgatgattga gcagcatgtc gaagcggagc
    22741 tggcgaacga tccgcccaac gatttaatgt ggtggcggcg tctgttccgg gcgattgata
    22801 agtgggcacc gccaggacag cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg
    22861 ctgaacgcag cgaaatgcag atcattcgta actttattgg tcaggccgat aacgccgatc
    22921 atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg
    22981 gcgaagataa ttaccaactt tatctgattc gtccggccag cagttctcaa tccgatttca
    23041 ttaacttact gtttgaccgc ccgctattac tgctgattgt caccatgttg gtcagtacgc
    23101 cgctgctgtt gtggttggcc tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg
    23161 ccgatgaagt tgcccaggga aacttacgcc agcacccgga actggaagcg gggccacagg
    23221 aattccttgc cgcaggtgcc agttttaacc agatggtcac cgcgctggag cgcatgatga
    23281 cctctcagca gcgtctgctt tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc
    23341 tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa ctcgagcgta
    23401 ttgaaaccga agcgcaacgt ctggacagca tgatcaacga tctgttggtg atgtcacgta
    23461 atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg tggagtgaag
    23521 tgctggataa cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc
    23581 cgcctgggcc gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca
    23641 ttgttcgtaa tgctctgcgt tattcccata cgaagattga agtgggcttt gcggtagata
    23701 aagacggtat caccattacg gtggacgacg atggtcctgg cgttagcccg gaagatcgcg
    23761 aacagatttt ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa tctggcggta
    23821 caggattggg gctggcgatt gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg
    23881 cagaagacag cccgctgggc ggtttacggc tggtgatttg gttgccgctg tataagcgga
    23941 gttaaactcc gcatttgtag gcaggataag gcgtttacgc cgcatccggc atttgagcag
    24001 gatgcctgat gcgacgctga tagcgtctta tcaggcctac actccggggg ttattttccc
    24061 cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc
    24121 gtccagcttt tcgataaccc ggcggcggag agtaaacggt gatactgatc gtcatcaaac
    24181 ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca catcgctgac acgggaaacc
    24241 aactccagcg gcgcgtccgc agacactttc cctggcgcta tcacgctata caaccacccc
    24301 accttacctg tgttttgcat caactgcgct atatcgctga tatcaaaatg gtaattgagt
    24361 ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg cctctcccca gcggaaaata
    24421 tcgcccatat gaacattact ttccgtgaga ccgtcggttg agagattttc gccaaacgca
    24481 ggcgcaacaa acaactccgc ctgttccgga aattcccgca cccagtagag atagtgctca
    24541 cgaggataat gacacagcgc tctgtctggc ccgccgtgaa cctttttctc cggctgctcg
    24601 tcaccttcca gccccagctc cgtcagcatc aactcaccat caacctggat tttggcaatt
    24661 gcgctgggtt tgccttcggg ataagcctga atcttgcctg tgtatacatc aaccggatat
    24721 cgcatctgtt ccctctccac gagaaatttc cccattagac catatttatt ttatgtcagg
    24781 cgtgctgttt tcgcggtaag tatccaggca ggaactcaat agcaaaccgg acgaacgtta
    24841 gttcatccgg ctgtggggat taaggaagca ggtttatttc accgtaccta aaatttcgat
    24901 tttcgctgct ctggctttga ctttacgtga ccagatagag gtaagaattg gcaccagaat
    24961 cgaagtcaca atgaccgccg tcgctaccag cgaagttgct gccggagcca tcggtttaaa
    25021 cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc gcggcccctg cggaactgga
    25081 agcggcaatt ccggcagtgc catcgccacc gccaatcaat ttatcggcga taatcaacgg
    25141 aataccggtc acgataatta ctgccacacc caacaggatc cccagcaaac cagtctgggc
    25201 aatcacagtc aaatcaatgg tattgcccag cgcgaaggca aagaatggaa tcagcgtttg
    25261 cactgctttg ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg caaagcccac
    25321 caggaacggt aatactgcgc cgacgaaaac atgcggttca aacgaggcaa tcccggcagt
    25381 gcccagaata atcatcgtca tgagcggccc ggactccaac gacatcaaca caaatgcccc
    25441 agcttcttct ttcgtaccgt actgctgcat gatggaagcg taaagtccgc cgttggtcat
    25501 atccatcgcc gccaccagcg ccagcgttga aagtccggca aagaatccaa cttcaacacc
    25561 atgttccgga ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg caattttagt
    25621 taccaccagc gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc
    25681 ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta ccggtaatca tcccgttggt
    25741 gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa
    25801 tagcgggacg agcatcatcc cccccgggat tttctcaatc gagcgtttta tctgcatttc
    25861 catcacctgt cggcatattt ttaagggctg atgcgtaatt cctcaatgga gcatcagtgt
    25921 tgatggaaac gaagatagac attcatttaa tcaataaaag tgatcaaaac cacatcaatt
    25981 gaaacgctgt tttattttta tcggatcatt gtttttattt tgaaattgat cacaaaaaaa
    26041 ccaccgccgt tggcgatggt tcatttttta agctgatatg cggcctatac gcctcattgc
    26101 agcaggcggc aaatgattat tttttcgccg caaaacgtgc cgctgcttcg tcccagttca
    26161 ccacgttcca gaactcttta atgtagtccg gacggcggtt ctggaatttc aggtagtaag
    26221 catgttccca cacatccagg cccagaatcg ggaagccgga agcgccagaa atagcttcac
    26281 ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc cagtttatcg cctttcagca
    26341 ccagccatgc ccagccggaa ccaaagcggg aagctgccgc tttttcaaat tctgctttga
    26401 agttatcaac ggagccgaag tcacgttcga tagccgcttt caggtcaccc tgcagggtgg
    26461 tgcctttttt cagacctttc cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc
    26521 gcagtacggt tttcttgtct gctggcagct ggtccagttt ggtgatcagc tcttcaaccg
    26581 gcaggttggc aaattctggc aggctttcca gcgccgcgtt ggcgttgttt acgtaggtct
    26641 gatggtgttt ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt tccagggcat
    26701 cgtaagcata cggcagggat ggcagggtat agctcatatt catctccagt attgtcgggc
    26761 ggccgattgt taatgccgcg taagcagttg gttcattata gttaattaaa tgatattgaa
    26821 aatgattatc aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat
    26881 gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa atcgacaacg gcggcaacag
    26941 gcgaaaggtt aatcgacagc acgattttta cactcatctc gtcggagatg tgaagcgacg
    27001 aaaaatgatg aggataagaa gatgagtaac gcgattacga tggggatatt ttggcatttg
    27061 atcggcgcgg ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa aaaatggtca
    27121 tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc gtggaccatc
    27181 agcgccctgt tactaccgga tttctgggcg tattacagct cgtttagtct ctctacgctg
    27241 ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta atatcaacta tggcctgacc
    27301 atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca ttggcattac gttgattgtc
    27361 ggtacgctga tgacgccaat tatcaacggc aatttcgatg tgctgattaa caccgaaggc
    27421 ggacgcatga cgttgctcgg cgttctggtg gcgctgattg gcgtagggat tgtaactcga
    27481 gccgggcagt tgaaagagcg caagatgggc attaaagccg aagagttcaa tctgaaaaaa
    27541 gggctggtgc tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac
    27601 gccgcaaaac cgatgcatga agccgctgcc gcacttggcg tcgatccact gtatgtcgct
    27661 ctgccaagct atgttgtcat catgggcggc ggcgcgatca ttaacctcgg tttctgtttt
    27721 attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg
    27781 ctgatcattc acaatgtgtt actctcggca ctgggcgggt tgatgtggta tctgcaattc
    27841 tttttctatg cctggggcca cgcccgcatt ccggcgcagt atgactacat cagttggatg
    27901 ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg
    27961 aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc
    28021 gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt gagatgacgc
    28081 cactggctgg gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata gttactatct
    28141 tcaaagccac attcggtcga aatatcactg attaacaggc ggctatgctg gagaagatat
    28201 tgcgcatgac acactctgac ctgtcgcaga tattgattga tggtcattcc agtctgctgg
    28261 cgaaattgct gacgcaaaac gcgctcactg cacgatgcct catcacaaaa tttatccagc
    28321 gcaaagggac ttttcaggct agccgccaac cgggtaatca gcttatccag caacgtttcg
    28381 ctggatgttg gcggcaacga atcactggtg taacgatggc gattcagcaa catcaccaac
    28441 tgcccgaaca gcaactcagc catttcgtta gcaaacgaca catgctgact actttcatgc
    28501 tcaagctgac cgataacctg ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt
    28561 ggttgccctg cgctggcgct aaatcccgga atcgctccct gtcagtcaag attcagcttc
    28621 agccgctccg ggcaataaat aatattctgc aaaaccagat cgttaacgga agcgtaggag
    28681 tgtttatcgt cagcatgaat ataaaagaga tcgccacggg taatgcgata aggacgatcg
    28741 ttgagtacat gcagaccatt accgcgccag acaatcacca gctcacaaaa atcatgtgta
    28801 tgttcggcaa agacatcttg cggataacgg tcagccacag cgactgcctg ctggtcgctg
    28861 gcaaaaaaat catctttgag aagttttaac tgatgcgcca ccgtggctac ctcggccaga
    28921 gaacgaagtt gattattcgc aatatggcgt acaaatacgt tgagaagatt cgcgttattg
    28981 cagaaagcca tcccgtccct ggcgaatatc acgcggtgac cagttaaact ctcggcgaaa
    29041 aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga taggcgatgt cagtaacgct
    29101 ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg
    29161 ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga
    29221 aaattgatcc gccacggcat cccaattcac ctcatcggca aaatggtcct ccagccaggc
    29281 cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg
    29341 cagcaagagc agtaattgca taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt
    29401 ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc acctgctgca atacgctgtg
    29461 gttaacgcgc cagtgagacg gatactgccc atccagctct tgtggcagca actgattcag
    29521 cccggcgaga aactgaaatc gatccggcga gcgatacagc acattggtca gacacagatt
    29581 atcggtatgt tcatacagat gccgatcatg agcgcgtacg aaacagaccg tgccaccggt
    29641 gatggtatag ggctgcccat taaacacatg aatacccgtg ccatgttcga caatcacaat
    29701 ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc gggagccggg gttctatcgc
    29761 cacggacgcg ttaccagacg gaaaaaaatc cacactatgt aatacggtca tactggcctc
    29821 ctgatgtcgt caacacggcg aaatagtaat cacgaggtca ggttcttacc ttaaattttc
    29881 gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat
    29941 gcggtgagca tcacatcacc acaatgaagc taattgtgaa catcatcacg ttcatctttc
    30001 cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt
    30061 tttagactgg tcgtaatgaa attcagcagg atcacattat gacctttcgc aattgtgtcg
    30121 ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc
    30181 gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc cagaacggtt
    30241 atgtcacctg gaatgtggat agcctggaaa gtgccattcg ccttggatta aacaaggtgt
    30301 gcgaggaagg gattcgtatc gatagcattg ggattgatac ctggggcgtg gactttgtgc
    30361 tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat agccgctcca
    30421 atggcctaat ggcgcaggca cagcaacaac tcggcaaacg cgatatttat caacgtagcg
    30481 gcatccagtt tctgcccttc aatacgcttt atcaactgcg tgcgctgacg gagcaacaac
    30541 ctgaacttat tccacacatt gctcacgctc tgctgatgcc ggattacttc agctatcgcc
    30601 tgaccggcaa gatgaactgg gagtacacca acgccacgac cacgcaactg gtcaatatca
    30661 atagcgacga ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa gcctggtttg
    30721 gtcgcccgac gcatccgggt aatgtcattg ggcactggat ttgcccgcag ggtaatgaga
    30781 ttccggtggt cgccgttgcc agccatgata ccgccagcgc ggttatcgcc tcgccgttaa
    30841 acggttcacg cgctgcttat ctctcttctg gcacctggtc attgatgggc ttcgaaagcc
    30901 agacgccatt taccaatgac acggcgctgg cagccaacat aaccaatgaa ggcggggcgg
    30961 aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg gctgcttcag cgagtgcttc
    31021 aggagcggca aatcaacgat ctcccggcgc ttatcgccgc gacacaggca cttccggcct
    31081 gccgcttcat catcaatccc aatgacgatc gctttattaa tcctgaggcg atgtgcagcg
    31141 aaattcaggc tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat gctgaactgg
    31201 cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc
    31261 agctacgcgg tgaagatttc tcgcaactgc atattgtcgg cggcggctgc cagaacacgc
    31321 tgctcaacca gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg cctgttgaag
    31381 cctcgacgct cggcaatatc ggcatccagt taatgacgct ggatgaactc aacaatgtgg
    31441 atgatttccg tcaggtcgtc agcaccaccg cgaatctgac cacctttacc cctaatcctg
    31501 acagtgaaat tgcccactat gtggcgcagc ttcactcgac acgacagaca aaggagcttt
    31561 gcgcatgacc actcaactgg aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt
    31621 ggggattgat gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt caatgcactg
    31681 ctggcagggc gatgatgttt ccggttttga aaacccggaa ggttcgctga ccggtgggat
    31741 tcaggctatt ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac gagccgatct
    31801 ggaacaggct atgcggctga ttccggggcc gaaacggctt aatttacatg ccatctatct
    31861 ggaatcagac acgccagtct cgcgcgacca gatcaaacct gagcacttca aaaactgggt
    31921 tgaatgggcg aaagccaatc agctcggtct ggattttaac ccctcctgtt tctcgcatcc
    31981 gctaagcgcc gatggcttta cgctctcgca tcccgacgac agcattcgcc agttctggat
    32041 tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc
    32101 atcggtgatg aacatctgga tcccggatgg tatgaaagat atcaccgttg accgtctcgc
    32161 cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc agcgagaagc tggatccggc
    32221 gcaccatatc gacgccgttg agagcaaatt gtttggcatt ggcgcagaga gctacacggt
    32281 tggctccaat gagttttaca tggggtatgc caccagccgc cagactgcgc tgtgcctgga
    32341 cgccgggcat ttccacccga ctgaagtgat ttccgacaag atttccgccg ccatgctgta
    32401 tgtaccgcag ttgctgctgc acgtcagccg tccggttcgc tgggacagcg atcacgtggt
    32461 gctgctggat gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg atctgttcga
    32521 ccgggtgcat atcggcctcg acttctttga tgcctctatc aaccgcattg ccgcgtgggt
    32581 cattggtaca cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga
    32641 gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac tggaagaaca
    32701 gaaatcgttg ccgtggcagg cggtctggga aatgtattgc caacgtcacg atacgccagc
    32761 aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg
    32821 gtaaacactg ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga atgaccatgc
    32881 gtgctgccac cctcaccccg gccctctccc tgaaagggcg agggagaaaa acatagcctc
    32941 gtgcggcaac ggagctgaac accgctccgg acagtcccct cgcccctacg gggagagggt
    33001 tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc aacctaattt acaggaacac
    33061 agaacatgca aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg
    33121 atgcctggct gaaaggctgg gacgagcgca atggtggtaa cctgacgctg cgcctggatg
    33181 acgccgatat cgcgccttat cacggcaact ttcatgcaca accgcgctat attcccctca
    33241 gccaacccat gcctttactg gcaaatacgc cgtttattgt caccggctcc ggcaaattct
    33301 tccgtaacgt ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg
    33361 gcgcgggcta ccatattctc tgggggttaa ccaacgaagc cgttccaact tccgaacttc
    33421 cggctcactt cctttcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga
    33481 tcatgcactg ccacgccacc aacctgatcg ccctcaccta tgtacttgaa aacgacaccg
    33541 cggtctttac tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg
    33601 gcgttggcat tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac
    33661 aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt
    33721 cgacgttgga tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag
    33781 tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc
    33841 tcggcaagcg cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa ggagcaaaca
    33901 tgatccgcaa agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc
    33961 ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact
    34021 acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg
    34081 aagaacggtg gaatgcggta gccagcactg atgtttgcca acgttggtgg aaatatatga
    34141 ccgatgttat gcccgctaac ccggataaca gcccggtgag tagcgagctg caagaagtgt
    34201 tttacctgcc gtaattgccc aatgtgccag agatgcctcc ggcacaatat tttaaatttc
    34261 gatttgaaaa attaaacata ttacatctcc cggtaagtta tatttccctg atacattgtg
    34321 agtaaatcac aaaaataatg aataacccat taatgattca tgtggtttat ttaaataacc
    34381 attatgtgca ttactccgca aatctgacct ttcactctgt ctcaaattgt cattgtttac
    34441 cgacatatcg cctctcatca cttaatcgct ccaccattac aattgattaa aatatatttt
    34501 atggagtccg attaatggca gctcttactg caagctgtat tgacctgaat attcagggca
    34561 atggcgctta ttccgttctg aagcagttgg cgacaatagc gttacaaaac ggttttatca
    34621 ccgactcaca ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac tctactggat
    34681 ttggttccgg tgtcgccgtg ccgcacggta aaagcgcctg cgttaaacaa ccgtttgtat
    34741 tattcgcccg caaagcgcag gctattgact ggaaagccag cgatggcgaa gacgtcaatt
    34801 gctggatctg cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa atcatcggca
    34861 cactgtgtcg caaaattatc caccaggatt ttattcatca actgcaacag ggcgacgccg
    34921 accaggtgct tgccttgtta aatcaaaccc tcagctcata aggaagtggc gatggagtca
    34981 tccttatgta ttgtcgcgat caccaactgc cccgccggga tcgctcacac ctacatggtg
    35041 gcggaagccc tggaacagaa agcccgttct ctcggccata ccataaaagt ggaaactcaa
    35101 gggtccagtg gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc cgattacgtc
    35161 attctcgcta ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc cgggaagaaa
    35221 gtttatgaga ttgccatctc ccaggcgttg aaaaatatcg accagatttt cagcgaatta
    35281 ccgacaaact cgcagctttt tgccgcagat agcggcgtga agcttggcaa acaggaagtg
    35341 caaagcggca gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct gccgtttgtc
    35401 atcggcggtg gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg tttgccgtat
    35461 accgatatgt cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat cggttacctt
    35521 ggcttcacct ttatgatccc catcatgggc gcttatattg cctcgtcgat tgccgataaa
    35581 ccggctttcg ctccggcgtt tctggtttgc tatctggcga acgacaaagc gctgctcggc
    35641 acccagtcgg gtgcgggatt cctcggcgcg gtggtgtttc tggtttgcta tctggcgaac
    35701 gacaaagcgc tgctcggcac ccagtcgggt gcgggattcc tcggcgcggt ggtgctgggg
    35761 ctggcgattg gctatttcgt cttctggttc cgcaaagtgc gccttggcaa agcattacaa
    35821 ccgctgctcg gttcaatgct gatcccgttc gttactctgc tggttttcgg cgtgctcact
    35881 tactacgtta tcgggccggt gatgtccgac ctcatgggcg ggctgctcca cttcctgaat
    35941 accattcctc catcaatgaa gtttgcggca gcgtttctgg tcggtgcgat gctggcgttc
    36001 gatatgggcg gccccatcaa taaaaccgcc tggttcttct gcttctcact gctggaaaaa
    36061 cacatttatg actggtacgc catcgtcggc gttgtcgcgt tgatgccgcc cgttgcggcg
    36121 ggtcttgcga cgttcattgc gcccaaactg tttacccgac aggaaaaaga ggctgccagt
    36181 agtgccattg tggtcggtgc cacagtcgca accgaaccgg ctatacctta cgctctggca
    36241 gctccgttgc cgatgattac cgccaatacg ctggcgggtg gcattaccgg cgtgctggtg
    36301 atcgctttcg gaatcaaacg tctggcacct ggtttgggta tctttgaccc gctgatcggc
    36361 ctgatgtcgc cggtgggttc gttttatctg gtgctagcga ttggcctcgc gctgaacatt
    36421 tcattcatta tcgtcctgaa aggattgtgg ttacgccgca aagcgaaagc cgcgcaacag
    36481 gagcttgtcc atgaacattg agttactgca acagttgtgc gaagccagcg ccgtcagcgg
    36541 cgatgaacag gaagttcgcg acattctgat aaacacgctg gaaccttgcg ttaatgagat
    36601 cacctttgat ggtctgggca gctttgttgc ccgtaagggg aataaaggtc caaaagttgc
    36661 cgttgtcagg catatggatg aagtcggctt tatggtcacc cacatcgacg agagcggttt
    36721 tctgcgcttt accaccattg gcggctggtg gaatcagtca atgctcaacc accgggtaac
    36781 catacgcaca cacaagggat ttaaaatccc tggtgtgatt ggttccgtcg cacctcatgc
    36841 gttaacggaa aagcaaaagc aacagccgct gtcatttgat gagatgttca ttgatattgg
    36901 cgcgaacagt cgcgatgagg tggaaaaacg cggtgtggcg atgggcgatt ttattagccc
    36961 ggaagccaat tttgcctgct ggggcgaaga taaagtggtc ggcaaggcgc tggataaccg
    37021 catcggctgc gcaatgatgg ctgaactatt gcagacggtg aataatcccg aaattacgct
    37081 gtatggcgtc ggcagcgtgg aagaagaagt tgggctacgc ggggcacaaa cctcggcgga
    37141 acacattaaa ccggatgtgg tgattgtgct ggataccgct gtcgcaggtg atgttccggg
    37201 cattgataac attaaatacc cgctgaaact gggccagggg ccggggctga tgctgtttga
    37261 caagcgctac ttccccaacc agaaactggt ggcggcgtta aaaagctgtg ccgcacataa
    37321 cgatttaccg ctgcaatttt ccaccatgaa aaccggtgcg acggatggcg ggcgctacaa
    37381 cgtgatgggc ggcgggcgtc cggttgttgc gctgtgcctg ccaacccgct atctgcacgc
    37441 taacagcggc atgatttcaa aagccgatta cgatgctctg ctcacgttga tacgggattt
    37501 tctgaccacc ttaaccgcgg agaaagtcaa cgcgtttagc cagttccgtc aggtggatta
    37561 atgctaaacg aacgccagtt aaagattgtc gatctgctgg agcaacagcc gcgcacgcct
    37621 ggtgagctgg cgcaacagac tggcgtttca ggcaggacca tcctgcgtga tattgactat
    37681 ctcaacttta cccttaacgg caaagcccgc attttcgcca gtggcagtgc gggctatcag
    37741 ctggaaatct tcgagcgccg cagctttttt cagttgctgc aaaagcacga taacgacgat
    37801 cggctgctgg cgctgttatt actgaatacc tttactcccc gtgcgcaact cgcctcggcg
    37861 cttaatttgc cagaaacctg ggtagcagag cgtctgcccc ggttaaaaca gcgttatgaa
    37921 cgcacttgtt gcctggccag tcgccccggt ttgggccatt tcattgatga gacagaagag
    37981 aaacgcgtta tcttgctggc gaacttgctg cgcaaagatc cgtttttaat tccgctggcg
    38041 ggcgtaacac gagacaattt tcagcattta tccacggcct gcgacaacca acaccgctgg
    38101 ccgctcatgc agggtgatta tctctccagc ctgattctgg cgatttacgc cctgcgtaat
    38161 caactgaccg atgagtggcc gcaatatccc ggtaacgaga taaaacaaat cgttgaacag
    38221 agcggcatgt ttcttggtga taacgctgta agaaccctga caggtttgat agagaaacag
    38281 catcagcaag cgcaggtaat ttcagccgat catgtgctgg ggttgctgca aagggtgcca
    38341 ggcatcgcgt cattgaatat tattgatacg cagctggttg agaatattac cgggcattta
    38401 ttacgttgcc ttgccgcacc ggtgtggatt gccgagcacc gccaaagcag catgaataac
    38461 ctgaaagccg cctggcctgc ggcctttgat atgagtctgc actttattac gctactgcgt
    38521 gaacagctcg atattcccct tttcgatagc gatctgatcg gtttgtattt tgcctgtgcg
    38581 ctggaacggc atcaaaacga acgccagccg atcattttgc tctcggacca gaacgcgatt
    38641 gccactatta atcagctcgc cattgagcgc gatgttttac attgtcgggt gattattgcc
    38701 cgtagcttaa gcgagcttat tgccattcgc gaagagattg agccgttatt gatcattaac
    38761 aacagccatt atttactgga tgacgcggta aataattaca tcaccgtaaa aaatatcatt
    38821 acggctgccg atatcgaaca aataaaacac tttctggcga cggcatttat tcgccaacag
    38881 ccggaacgct ttttctctgc ccccggaagt tttcattatt cgaatgtacg cggtgaaagc
    38941 tggcaacata ttacccggca aatttgtgcg caattagtgg cacaacacca tattaccgcc
    39001 gatgaagcgc aacggatcat cgcccgcgaa ggcgaaggtg aaaacctgat tgttaatcgc
    39061 ctcgccatcc cacattgctg gagcgaacag gagcgacgtt ttcgtggatt ttttattacc
    39121 ctcgcccaac cagttgaggt gaataacgaa gtcattaacc atgtcttgat cgcctgcgcc
    39181 gccgccgatg cgcgtcatga gctaaaaata tttagctatc tggcaagcgt attgtgtcag
    39241 catccggcag aagttattgc cgggttaaca ggatatgagg catttatgga gttacttcac
    39301 aaggggtgaa ctgtgtcgga tgcgccgcat ccgacactgc attttcatca aaaacgttta
    39361 acgcaggcta ttatagtcat caaccatttc gttgtactcg cgtaacgctc gtggaaatga
    39421 gtcttcgacc atccagcttg tgtttgcatc ctgaagatgc tctttatccc agtcagagta
    39481 cgggacttta tctctgacgc ggcgaacata ttttttagtc tcgagttgat actggtctgc
    39541 cgaattaatg aacgagaaat tcataccgcc tttatccgct tctttggcct gcgctaccag
    39601 cgtttccagt tcagagactt tcttcattgc cgcatcaaca tcaa
//
LOCUS       DAZOXR010000013        38613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZOXR010000013 DAZOXR010000000
VERSION     DAZOXR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.013"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHUNN_000609"
     CDS             complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHUNN_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR0575077.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHUNN_000610"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHUNN_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR0575078.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHUNN_000611"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHUNN_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0575079.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHUNN_000612"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHUNN_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0575080.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHUNN_000613"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHUNN_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0575081.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHUNN_000614"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHUNN_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR0575082.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHUNN_000615"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHUNN_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0575083.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHUNN_000616"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHUNN_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0575084.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHUNN_000617"
                     /pseudo
     CDS             complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHUNN_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHUNN_000618"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHUNN_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0575085.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHUNN_000619"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHUNN_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR0575086.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHUNN_000620"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHUNN_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0575087.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHUNN_000621"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHUNN_000621"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0575088.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="ACHUNN_000622"
     CDS             complement(14170..14742)
                     /locus_tag="ACHUNN_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0575089.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="ACHUNN_000623"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="ACHUNN_000623"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0575090.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="ACHUNN_000624"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="ACHUNN_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0575091.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="ACHUNN_000625"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="ACHUNN_000625"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0575092.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="ACHUNN_000626"
     CDS             17911..18759
                     /locus_tag="ACHUNN_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0575093.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHUNN_000627"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHUNN_000627"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0575094.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHUNN_000628"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHUNN_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0575095.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="ACHUNN_000629"
     CDS             complement(21671..21871)
                     /locus_tag="ACHUNN_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0575096.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHUNN_000630"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHUNN_000630"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0575097.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="ACHUNN_000631"
     CDS             complement(23030..23386)
                     /locus_tag="ACHUNN_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0575098.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="ACHUNN_000632"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="ACHUNN_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0575099.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="ACHUNN_000633"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="ACHUNN_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0575100.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="ACHUNN_000634"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="ACHUNN_000634"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR0575101.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="ACHUNN_000635"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="ACHUNN_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0575102.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="ACHUNN_000636"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="ACHUNN_000636"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR0575103.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="ACHUNN_000637"
     CDS             31857..32900
                     /locus_tag="ACHUNN_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0575104.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="ACHUNN_000638"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="ACHUNN_000638"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0575105.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="ACHUNN_000639"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="ACHUNN_000639"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0575106.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="ACHUNN_000640"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="ACHUNN_000640"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0575107.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT         9517 a        10172 c        10277 g         8647 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgacgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggtggtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa aatcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt att
//
LOCUS       DAZOXR010000014        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZOXR010000014 DAZOXR010000000
VERSION     DAZOXR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.014"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUNN_000641"
     CDS             59..1423
                     /locus_tag="ACHUNN_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575108.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUNN_000642"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUNN_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0575109.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUNN_000643"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUNN_000643"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0575110.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUNN_000644"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUNN_000644"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0575111.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUNN_000645"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUNN_000645"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0575112.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUNN_000646"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUNN_000646"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0575113.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUNN_000647"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUNN_000647"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0575114.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUNN_000648"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUNN_000648"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0575115.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUNN_000649"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUNN_000649"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0575116.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUNN_000650"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUNN_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0575117.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUNN_000651"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUNN_000651"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0575118.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUNN_000652"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUNN_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0575119.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUNN_000653"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUNN_000653"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0575120.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUNN_000654"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUNN_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0575121.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUNN_000655"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUNN_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0575122.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHUNN_000656"
     CDS             13749..14288
                     /locus_tag="ACHUNN_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0575123.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUNN_000657"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUNN_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0575124.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUNN_000658"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUNN_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0575125.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUNN_000659"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUNN_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0575126.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUNN_000660"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUNN_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0575127.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUNN_000661"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUNN_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0575128.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUNN_000662"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUNN_000662"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0575129.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUNN_000663"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUNN_000663"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0575130.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUNN_000664"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUNN_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR0575131.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUNN_000665"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUNN_000665"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0575132.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUNN_000666"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUNN_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0575133.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUNN_000667"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUNN_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0575134.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUNN_000668"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUNN_000668"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0575135.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUNN_000669"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUNN_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0575136.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUNN_000670"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUNN_000670"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0575137.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUNN_000671"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUNN_000671"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0575138.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUNN_000672"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUNN_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0575139.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUNN_000673"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUNN_000673"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0575140.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUNN_000674"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUNN_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0575141.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHUNN_000675"
     CDS             complement(37465..37920)
                     /locus_tag="ACHUNN_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575142.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHUNN_000676"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHUNN_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgcctggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZOXR010000015        37811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZOXR010000015 DAZOXR010000000
VERSION     DAZOXR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.015"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(270..887)
                     /gene="tdk"
                     /locus_tag="ACHUNN_000677"
     CDS             complement(270..887)
                     /gene="tdk"
                     /locus_tag="ACHUNN_000677"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0575143.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1492..1905
                     /gene="hns"
                     /locus_tag="ACHUNN_000678"
     CDS             1492..1905
                     /gene="hns"
                     /locus_tag="ACHUNN_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0575144.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2049..2957)
                     /gene="galU"
                     /locus_tag="ACHUNN_000679"
     CDS             complement(2049..2957)
                     /gene="galU"
                     /locus_tag="ACHUNN_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0575145.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3159..4172)
                     /gene="rssB"
                     /locus_tag="ACHUNN_000680"
     CDS             complement(3159..4172)
                     /gene="rssB"
                     /locus_tag="ACHUNN_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0575146.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4264..5169)
                     /gene="rssA"
                     /locus_tag="ACHUNN_000681"
     CDS             complement(4264..5169)
                     /gene="rssA"
                     /locus_tag="ACHUNN_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0575147.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5282..5740
                     /locus_tag="ACHUNN_000682"
     CDS             5282..5740
                     /locus_tag="ACHUNN_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0575148.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5790..6632
                     /gene="purU"
                     /locus_tag="ACHUNN_000683"
     CDS             5790..6632
                     /gene="purU"
                     /locus_tag="ACHUNN_000683"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0575149.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6792..6876
                     /locus_tag="ACHUNN_000684"
     tRNA            6792..6876
                     /locus_tag="ACHUNN_000684"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6826..6828,aa:Tyr,seq:gta)
     gene            6924..7055
                     /locus_tag="ACHUNN_000685"
     ncRNA           6924..7055
                     /ncRNA_class="other"
                     /locus_tag="ACHUNN_000685"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7239..7916)
                     /gene="narI"
                     /locus_tag="ACHUNN_000686"
     CDS             complement(7239..7916)
                     /gene="narI"
                     /locus_tag="ACHUNN_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0575150.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="ACHUNN_000687"
     CDS             complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="ACHUNN_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0575151.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8623..10161)
                     /gene="narH"
                     /locus_tag="ACHUNN_000688"
     CDS             complement(8623..10161)
                     /gene="narH"
                     /locus_tag="ACHUNN_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0575152.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10158..13901)
                     /locus_tag="ACHUNN_000689"
     CDS             complement(10158..13901)
                     /locus_tag="ACHUNN_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0575153.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14294..15685)
                     /gene="narK"
                     /locus_tag="ACHUNN_000690"
     CDS             complement(14294..15685)
                     /gene="narK"
                     /locus_tag="ACHUNN_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0575154.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16024..17820
                     /gene="narX"
                     /locus_tag="ACHUNN_000691"
     CDS             16024..17820
                     /gene="narX"
                     /locus_tag="ACHUNN_000691"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0575155.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17813..18463
                     /gene="narL"
                     /locus_tag="ACHUNN_000692"
     CDS             17813..18463
                     /gene="narL"
                     /locus_tag="ACHUNN_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0575156.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="ACHUNN_000693"
                     /gene_synonym="iatA"
     CDS             complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="ACHUNN_000693"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0575157.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20043..20396
                     /gene="ychN"
                     /locus_tag="ACHUNN_000694"
     CDS             20043..20396
                     /gene="ychN"
                     /locus_tag="ACHUNN_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0575158.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="ACHUNN_000695"
     CDS             complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="ACHUNN_000695"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0575159.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="ACHUNN_000696"
     CDS             complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="ACHUNN_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0575160.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21793..22893
                     /gene="chaA"
                     /locus_tag="ACHUNN_000697"
     CDS             21793..22893
                     /gene="chaA"
                     /locus_tag="ACHUNN_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0575161.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23296..23403
                     /locus_tag="ACHUNN_000698"
     CDS             23296..23403
                     /locus_tag="ACHUNN_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0575162.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23832..23939
                     /locus_tag="ACHUNN_000699"
     CDS             23832..23939
                     /locus_tag="ACHUNN_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0575163.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24367..24474
                     /locus_tag="ACHUNN_000700"
     CDS             24367..24474
                     /locus_tag="ACHUNN_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0575164.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24623..25477)
                     /gene="kdsA"
                     /locus_tag="ACHUNN_000701"
     CDS             complement(24623..25477)
                     /gene="kdsA"
                     /locus_tag="ACHUNN_000701"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0575165.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(25513..26321)
                     /gene="sirB1"
                     /locus_tag="ACHUNN_000702"
                     /pseudo
     CDS             complement(25513..26321)
                     /gene="sirB1"
                     /locus_tag="ACHUNN_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            complement(26325..26717)
                     /gene="sirB2"
                     /locus_tag="ACHUNN_000703"
     CDS             complement(26325..26717)
                     /gene="sirB2"
                     /locus_tag="ACHUNN_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0575166.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26714..27547)
                     /gene="prmC"
                     /locus_tag="ACHUNN_000704"
     CDS             complement(26714..27547)
                     /gene="prmC"
                     /locus_tag="ACHUNN_000704"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0575167.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27547..28629)
                     /gene="prfA"
                     /locus_tag="ACHUNN_000705"
     CDS             complement(27547..28629)
                     /gene="prfA"
                     /locus_tag="ACHUNN_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0575168.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28671..29927)
                     /gene="hemA"
                     /locus_tag="ACHUNN_000706"
     CDS             complement(28671..29927)
                     /gene="hemA"
                     /locus_tag="ACHUNN_000706"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0575169.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30141..30764
                     /gene="lolB"
                     /locus_tag="ACHUNN_000707"
     CDS             30141..30764
                     /gene="lolB"
                     /locus_tag="ACHUNN_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0575170.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30764..31615
                     /gene="ispE"
                     /locus_tag="ACHUNN_000708"
     CDS             30764..31615
                     /gene="ispE"
                     /locus_tag="ACHUNN_000708"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0575171.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31766..32713
                     /gene="prs"
                     /locus_tag="ACHUNN_000709"
     CDS             31766..32713
                     /gene="prs"
                     /locus_tag="ACHUNN_000709"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0575172.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32837..34516
                     /gene="dauA"
                     /locus_tag="ACHUNN_000710"
     CDS             32837..34516
                     /gene="dauA"
                     /locus_tag="ACHUNN_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0575173.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34571..34849)
                     /gene="ychH"
                     /locus_tag="ACHUNN_000711"
     CDS             complement(34571..34849)
                     /gene="ychH"
                     /locus_tag="ACHUNN_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0575174.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35126..35710
                     /gene="pth"
                     /locus_tag="ACHUNN_000712"
     CDS             35126..35710
                     /gene="pth"
                     /locus_tag="ACHUNN_000712"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0575175.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35827..36918
                     /gene="ychF"
                     /locus_tag="ACHUNN_000713"
     CDS             35827..36918
                     /gene="ychF"
                     /locus_tag="ACHUNN_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0575176.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9411 a         9791 c         9241 g         9368 t
ORIGIN      
        1 cttctggggc cgtttttttg tttacccaaa gcaacttttc cataaaccga cagcattagc
       61 cttcatcata tttgcgacga tgtataacgc ctaaacacag ggatattgta ctttacaggt
      121 cacaagtcaa cgtcggtgct taagagccct gtgaggcgta tagcggcgtt aaaaaactgc
      181 cgagaagggt atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc
      241 tgctcaccct gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc
      301 gttaatgagc cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt
      361 tcattaccac caattaccac ctgctcacct tcgttataag gtctgcctgc ttgatcaaga
      421 cgcagcacca tgcttgcttt acggccacaa aaacagatgg tttttaattc aaccagtttg
      481 tcggaccatg ccagtaagta ttggctgcca ataaataatt cacctcgaaa atcggtacgt
      541 aaaccataac aaagtacggg tatatcgagt tgatcgacaa cctccgataa ttcatatact
      601 tgttgtctgg ttaaaaactg gcattcatca accagtacgc aatgaattgc ctgctgttca
      661 tgttccgcac gaatctcatc aaataatgat gaattttggt taaataattt tgcaggcgat
      721 gacaaaccta tacgcgaact gactttcccg gcaccaaagc gatcatcaat ttctgccgta
      781 tatacgacag tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa caatgctgta
      841 gacttacccg cattcattgc ggaatagtag aaatatagct gtgccatcag ccacaggccc
      901 tcaatgatat gaaataaagt tggctggagt ttatcataat tcgtcagttt ttcagctcaa
      961 cagtatgcac agaagtgggt atatgcgttc tcccttacga agccttgcat aatccttctg
     1021 agctatcatt acaactgcct cgcttgttat aagcgggtaa atgactactg gtaactattc
     1081 acaatcttta acctgttgcg caagtaatag ccctctgttg acctccagga gatagtgcaa
     1141 tactaagtcc atgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat
     1201 tctcgtaaac acaactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca
     1261 taaaatgtga catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtgtaaa
     1321 tagggctata tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac
     1381 aaaataaaga acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg
     1441 ctctattatt acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa
     1501 gcacttaaaa ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt
     1561 gaaacgctgg aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa
     1621 gaaagcgcgg ctgctgctga agttgaagag cgcactcgta agctgcagca atatcgcgaa
     1681 atgctgatcg ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa
     1741 tctggcacca aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac
     1801 ggcgaaacta aaacctggac tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg
     1861 gatgagcaag gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc
     1921 acttgcttaa aatcccgcca gcggcgggat tttttattgt ccggtttaag acaatttaat
     1981 aagaataagc tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga
     2041 tacgaatgtt acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca
     2101 agggtgttat gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg
     2161 cagtcatggc tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata
     2221 tcaattgcgt cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac
     2281 ggccaaatat ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca
     2341 tccgctttcg gtttttctac cacaccaacc atcggtacgc tttcacccgg cgctaattca
     2401 acgcctttgc aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc
     2461 tggctatgac ccgtttcatc aaagcggcgg atcatctctg ccaggttatc ctgtgacaaa
     2521 tcggattcat attcatccag aataacatca ggcaaaataa cagctaccgg ttcatcaccc
     2581 actaccgggt gagcacacaa taccgcgtgt cccaggcctt tcgccagacc ctgacgaact
     2641 tgcataatag tcacgtgcgg tggacaaata gactgcactt catcaagcag ttgacgtttt
     2701 acacgttttt ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag
     2761 tttttagatg agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc
     2821 acgacgtatt gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc
     2881 gtcgccggca acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc
     2941 gtattaatgg cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt
     3001 tctgtatcgc atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga
     3061 gttagcataa ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat
     3121 tagcaggtaa tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag
     3181 cgcagtcgac caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat
     3241 tgattcaaat aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg
     3301 tgttcgccgg tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt
     3361 tcgcgatgat aatagccaac taatagcgga aactgccccg gcagattggc ctgacggagt
     3421 aaatggttta cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca
     3481 agctgttcct gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca
     3541 ttatgtccag ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg
     3601 gcaatatcaa gcaccaggcc gggtttgtcc gcggcaacca attgacgata attaacccgg
     3661 caatgggaaa tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg
     3721 gcagggttat caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc
     3781 gaattaaaca tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct
     3841 ttaactggtt tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc
     3901 atattttcag tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc
     3961 tccagcagtt taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga
     4021 gtgaaacctc ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct
     4081 cccaatgagg aaaaccatga atccagaagc gagcgaaata cctgctcatc ttcaacaatg
     4141 agaatctgtt ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa
     4201 tagtggcatg ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc
     4261 tggtcaaatg ttggtgcgta ccaacggcaa aagttcgtcc attttccttt ccactgccag
     4321 ctgtccggcc gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca
     4381 aacaggttga atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt
     4441 ctccaacacc tggatagacg tggtcataat ctctgttgcc gttggcgctg tcacagcccg
     4501 acgtgtcgtt atgctgccta acctttcttt cagacgcgca tgccacggta gagaatcacc
     4561 attctcgcta ttttcttcac tgacattaaa ggagagcaga tcttgttgca tcaaatgagc
     4621 atcgtgctgc aggtcgaccg ctatcacaat atcagccccc aatgcacgcg taagagaaat
     4681 aggaattggg ttaactactg ccccatcaac cagccagtag ccgttatgtg caacgggtgc
     4741 catgagtcct ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa
     4801 ccataattca cgccccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt
     4861 ttcgatctct gtttccggca ttatttcgcg atattgattg aagacacgct cgccgcgcag
     4921 taacccaccg cgctgccagg agaggtccat caggcgtaaa acgtcccaat aactgaaaga
     4981 ggttacccaa tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga
     5041 accaattgaa catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat
     5101 aacgccaata tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat
     5161 ctttctcatt attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct
     5221 cggttaacat agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt
     5281 tgtgtctcag ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta
     5341 tgtgtctggt gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc
     5401 ttttgtgatg caagacgcag attatttaat aaagacctgg catccctctt gtggagcagc
     5461 ggcgttacgt gccgaattga tggccggatt tgcgcatact gaatggttgg gattgaccgt
     5521 atttgaacat tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt
     5581 taccgaaggt ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg
     5641 tcaatggtac tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg
     5701 tggttcaggt aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag
     5761 caaacactct caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta
     5821 ctatttgtcc ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg
     5881 agttaaatat cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc
     5941 gcacggaact ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat
     6001 tgccagaagg ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg
     6061 tcactaaaga agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggcctgg
     6121 atgtcgaaat cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt
     6181 ttgatattcc gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa
     6241 agatggcgga agccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc
     6301 gggtattaac gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt
     6361 cattcctgcc tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga
     6421 agattattgg cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca
     6481 tgcaggacgt tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc
     6541 gtgacgtcga gaaaaacgtc ttaagtcgtg cgctatacaa agtactggca cagcgcgtct
     6601 ttgtttacgg taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt
     6661 aatccgttac ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag
     6721 cggcgcgtca tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag
     6781 cattaccccg tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca
     6841 tcgacttcga aggttcgaat ccttccccca ccaccatcac ttttcaaaag tccgtaaact
     6901 ctcaaacgaa tccgcaatta aatattctgc ccaagcgggg aaggatgaga agcttcgacc
     6961 aaggttcgac ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa
     7021 gcgcgcagcg ctgagtaatc cttcccccac caccatcgct tccttaaata atcaaaacct
     7081 gttactccga gctaattttg cccgatggca ccacgtttac cgggcctaca atatcctccc
     7141 ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc ccataaaaaa
     7201 accccgccga agcagggtct atgtgaacta aaattcgctt agtgacgagc gcgcaccagc
     7261 tggtacttac gtgtcagata ctccaccggt acgctccaga tgtgtaccag acgcgagaac
     7321 gggaacagca ggaataacgt catccccagc accaggtgca gacggaagat aaacgccaca
     7381 ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca ccgactgcgc ccagccaacc
     7441 agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt caacaggccc
     7501 agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt agtggttgca
     7561 cgcacgcgtg ggctgaacag acgacgcttc agcaacagca cgccgccaat cagacacagc
     7621 acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat cggcagccag
     7681 gcttcataca tccagtgcgg cgtcagcata ccgaagaagt gaccaacaaa aatccccaga
     7741 ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag catctggctg
     7801 gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc aatcaggaag
     7861 accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa ttgcattagt
     7921 gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca aagcgacgct
     7981 gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta acctgctctt
     8041 cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg gcaatttttt
     8101 ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga tcaaacatca
     8161 cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga atcggcgcga
     8221 tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac tccagataca
     8281 gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa gccgtgctgc tcgtactgcg
     8341 ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca tgttcaaata
     8401 gcaacagtga cgtggcgcga ccacggtcga acagttcgct gtactgggcc tgggcatcga
     8461 gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca tcttctttcg
     8521 acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc cataaggcag
     8581 catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat gcggctccgt
     8641 tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga atttggtatc
     8701 tgaaccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg ggaaggcttc
     8761 ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt tagcaatcgc
     8821 cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt ccagcgcacg
     8881 ggtatcaact ttaccgtcaa cggtttcagc acgtttgtaa tgacgcatcg ccagcatacg
     8941 tttcagtgcg cgcagtaccg gtttggtatc accggcggtc agcagattcg ccagatactg
     9001 taccggaata cgcaagcttt cgacgtctgg cagaatgccg ttgctaccca gctcacccgc
     9061 gtctgctgca gactgaatcg gagacagagg cggcacgtac cagaccatcg gcagtgtgcg
     9121 atattctgga tgcagcggca gcgccagttt ccattccatt gccattttat aaaccggcga
     9181 ctgctgtgcg gcttcaataa cgctcagcgg aataccgtct ttaatcgcct gctcgatgac
     9241 tttcggatcg ttcggatcga ggaacacctc cagctgacgc tggtaaagat ctttctcgtt
     9301 ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc caagataacg
     9361 gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa tacgcggata
     9421 gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt ttttgtacgg
     9481 gcatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca ggacgatgcc
     9541 atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg ccgggttcag
     9601 gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact ggctatacat
     9661 cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttgtcaaact caccacccag
     9721 gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg taatcagcga
     9781 acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca ggttctgata
     9841 gtcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt tagcgaagat
     9901 tttacccagc aacatggcac ggttacccat gcgcggctgc agtttgccgt tgattttacg
     9961 gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc cctgacccgg
    10021 cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg tccagacgtt
    10081 tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca gcaccatgcc
    10141 gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc ttcgccatct
    10201 aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt agaacctacg
    10261 gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat atgcgtcggt
    10321 ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt aatttccgaa
    10381 ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc tgccggaaca
    10441 cgctggctga caaccgcacg ggcagtcaga gcaccgttgc tgttgaatac ttcaatccag
    10501 tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac caccggacca
    10561 ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat accccacttc
    10621 tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt ctggcctatc
    10681 acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct ttcaccgaaa
    10741 tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt acgccatggg
    10801 atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc ttccagacca
    10861 gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgag gaagcggatc
    10921 ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa ttcgctcagg
    10981 gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagtgt caggatcatc
    11041 tctgccgcat caattgcggt gttcagcatc ggctggcctt tcgccggacc ttccgctttg
    11101 gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc aatcccttta
    11161 ccgccattac cgattttctc catcaacggg ccaatagagg taaagcgttc gtaagtcgcc
    11221 ggataatcgc gctctacgac cataatgtgt ggcgcggttt tacctgggat caggtcgcac
    11281 tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc agagtcatgc
    11341 tggataggca gcgtgacgat gtcggtttct ttacccagat ggccgacgca cacttcggag
    11401 aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tcgcttccca ggccggatcg
    11461 accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat gtcgtctttt
    11521 tcgtaccaag tcgccgtcgg caaaatgatg tcggaataga gacaggtgct cgacagacgg
    11581 aagtccagcg taaccaccag atccagcttg ccttccagac cattgtcctg ccagtccact
    11641 tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc gtgctccgtc
    11701 cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag caggttagaa
    11761 cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc tgccgcaaaa
    11821 cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat cccggctttt
    11881 tccgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggtgcaga cggcagccag
    11941 cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc cggtatagcg ggatttgtcc
    12001 gccatcggtg acagcaactc ttccgccgtg acggtttcat aacgccactg gctggagtgg
    12061 ttatagaaat aagaagtgct gttcatgtga cgcgccggac gctgccagtc aagggcaaac
    12121 gccagcggct gccagccggt ttgcggacgc agtttttcct gacctacata gtgcgcccag
    12181 ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag accacgatag
    12241 ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat cgaacgaccg
    12301 tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg gctgcgagaa
    12361 acgccggtaa tctgctcggc ccaggccggg gtataagctt tcacatcgtc atagctggtt
    12421 gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt cagatcataa
    12481 acggtgctca ccagggcggt gctgccatca gccagttgca ggcgtttcac cggcagtttg
    12541 tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc gccaccaaag
    12601 tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag ttgcagctcg
    12661 gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt ctcgccccag
    12721 cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa ggcgacagtt
    12781 ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc gcgcagcata
    12841 cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg catgtcggtg
    12901 tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa ttcacgcagc
    12961 attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg tgccagccac
    13021 agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt tttggtgcct
    13081 ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg cacgtttgaa
    13141 ccccaggcga tgatgtagct ggagttatac cagtcagcag attccggtac gtcagtttgc
    13201 tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt accagtcgta gaagcttaag
    13261 caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac catcgacatt
    13321 gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat ggtgtaaacg
    13381 ttagatgcgg cgatcagttc gttcacctcc tgccaggaag aacgaacaaa tccaccgcgt
    13441 ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga agcccatgcc
    13501 tcaaccggat cgctatgcag cgccttcgct tcacgccaca ttttcatcag gcgtttgcgc
    13561 atcatcgggt atttcaggcg gttggcactg taaagatacc aggagtagct ggcaccgcgc
    13621 gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata gtcagtctgc
    13681 tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca ggagccggtg
    13741 cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg gcgatatcca
    13801 tcctcccagt cacggttggt attgagaagc tggccatgcc catcggcaaa ggtttcaccc
    13861 ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct cctgtgggag
    13921 cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaaggta acgctctgaa
    13981 acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag taaattccta
    14041 agatacccct ttaggataat taacagccac cacataacag actgtaaatt aagacgtttt
    14101 atacctgtag ttatttaaac tattaagatt tttatgcaga tgggtattaa ggagtattcc
    14161 ccatgggtaa catattaatg gctcttaatg aaatccggcg aaataagcca tataatatga
    14221 gtatgttgta actaaatgac gcataaaaaa agcgcgacat catgccgcgc aaaggataat
    14281 caaatattaa gatttatttt ttagaatgcc gaccatatac cgcccaggta atcactacgc
    14341 aggcgatata gaaaatcaaa aataccttca ttgcgccgac tggcgaaccc gttaatgcca
    14401 gcgagctacc aaacgctttc gggataaaga agccaccaat agcgccaatc gcagagatga
    14461 aacccagcgc cgctgccgtg tcggttgccg cttcacgcat cgcacgttcg tcagaacccc
    14521 cttctgcttt cacgcgatcc attgtcagtt tacggaagat cactgaaatc atctggaaag
    14581 tggaaccact acccagccca gctgtcagga acagcgccag gaagaccgcg aagaacgcca
    14641 tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa cagcaggccg ctgaaaatcg
    14701 ccatcagaat aaagttcacc agcgtgacac gagttccgcc cagacggtca gataatgcac
    14761 cacctgcaga acgcgccagc gcaccaataa acggcccgaa gaaagcgtat tgcagaatct
    14821 gaacatccgg gaactgcgtt tttgacagca tcgcaaagcc cgcggagaag ccgataaagg
    14881 agccgaaggt tgccagatac agcaggctca taatccacag atgaccccgt ttgagtaccg
    14941 gcaactgctc cttgatggag gctttcgagg tagcaagatc gttcatgcca aaccacgccg
    15001 caatggtgaa gatggcaagg aacggcaccc atatccagga cgcattcgcc agatacagct
    15061 cagtcccatc cggctgtttg acgccctggc taccaaatac tgcgaaaatc gacagtgata
    15121 ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat gtttcccaga ccaccattca
    15181 gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt gccatactgg
    15241 atgcgaagtt cgcgccagca aagccgcata gcagagagat gatgatgaag acgctataag
    15301 gcgtggaggt atcctgcacg gcaaaaccca gccagacgca aggaataatc agaataccgg
    15361 tgctgaacgc cgtccagcga cgaccaccga agataggaac cataaaggag tatggaacac
    15421 gtaataacgc gccagaaacc gaaggcagcg cagtcaacat aaatagctga tcggtcgtaa
    15481 aattaaaacc gactttcggt aagttcaccg caacagcgct gaacaacatc catacgcaaa
    15541 acgccagcag cagacaggga acggaaatcc acaggttacg gctggcaata cgttgaccgc
    15601 gttgttgcca gaacgcagga tcttccggtc gccaatctgt aatgacagct ccagtagccc
    15661 tttcgggggc ggatgagtga ctcatagaca cctctgatac tcgtttcgat gtctgccacc
    15721 ttagtgtctg tagctaaagg caatttgatg taaatcaaac gataagataa ctttatctat
    15781 cattgatatt tatcattacc catagtgagt acagtgattt cataaaaatt atgagatttt
    15841 tcacggtgct gtaaaatccc tacccttacc gatgtaaagc gactaaccac acggcaaata
    15901 aggagtaact ctttccgggt atgggtatac ttcagccaat agccgagaat actgccattc
    15961 cagaatgtat cgtcacattc attaaggtta ttgctcattt aaagcctgaa ggaagaggtt
    16021 tgcatgctta aacgttgtct ctctccgctc accctggtta atcaggttgc gcttattgtg
    16081 ttgctttcta ctgctattgg actggcaggg atggcggttt ctggctggct ggtgcaaggc
    16141 gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca aagttaccgt
    16201 ctgttggcgg cagtgccatt aagtgagaaa gacaagccct taattaaaga gatggaacaa
    16261 acggcattta gcgccgagtt aactcgagca gcagaacgag acgaacaact ggcgcaatta
    16321 cagggtttac aagattactg gcgtaatgaa ctgatccctg cactgatgcg tgcacaaaac
    16381 cgcgaaacgg tgtcagcgga tgtcagccag tttgttgccg ggcttgatca gctggtatct
    16441 ggttttgacc gcaccacgga aatgcgcatt gagacagtgg tactggtcca tcgggtaatg
    16501 gcggtattta tggcactttt actggtgttc actattatct ggttgcgggc gcgactgcta
    16561 caaccgtggc ggcaactgct ggcaatggcg agtgccgtca gtcatcgcga ttttacccaa
    16621 cgcgcgaaca tcagcgggcg caacgaaatg gcgatgcttg gaactgcatt gaacaatatg
    16681 tctgcagaac tggccgaaag ttatgccgta cttgagcagc gggttcagga gaaaaccgcc
    16741 gggctggagc ataaaaatca gatcctctct tttttatggc aggctaaccg ccgtttgcat
    16801 tcccgcgccc cgctgtgtga acgcctgtca cctgtactca acggcttaca gaatttaacc
    16861 ctgctacgtg atatcgaatt gcgggtgtat gacactgatg atgaagagaa tcatcaggag
    16921 tttacctgcc agccagatat gacttgtgat gataaaggct gccagctctg cccgcgcggc
    16981 gtattacccg ttggtgatcg cggcacgacc ctgaagtggc ggctggctga ctctcatacg
    17041 cagtacggta ttttgctggc gaccctgccg caggggcgtc atcttagcca tgatcaacaa
    17101 caactgctgg ataccctggt tgaacaactc accgccacgc tggcgctgga tcgccatcag
    17161 gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg cgaactgcat
    17221 gattctattg cccaatctct ctcttgcatg aagatgcagg tgagttgttt acagatgcag
    17281 ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa cgaactgaat
    17341 gcatcctggg cgcagttgcg tgaattgctc accacattcc gcttgcagct caccgagcct
    17401 ggattacgtc cggcgctgga ggcgagttgc gaagagtaca gcgccaaatt tggcttcccg
    17461 gtgaagctgg attatcaatt gccgcctcgc ctggtgcctt cgcatcaggc aatccacttg
    17521 ttgcaaattg cccgtgaggc attaagtaac gccctcaaac attcgcaagc gagtgaagtc
    17581 gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga ccgtccagga taacggctgc
    17641 ggcgtgcctg aaaatgccat ccgcagcaat cactacggca tgataataat gcgcgaccgt
    17701 gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg caccgaagtg
    17761 gtggtcacct ttattcccga aaaaactttc acagacgtcc aaggagatac ccatgagtaa
    17821 tcaggaaccg gctactatcc tgctgattga cgatcacccg atgctgcgaa ctggcgtaaa
    17881 acagcttatc agtatggcac cagatatcac cgtggttggc gaagcgagta atggcgaaca
    17941 gggtattgaa ctggcggaat ctcttgatcc cgatctgatc ctgttagatc tcaatatgcc
    18001 cggcatgaac ggtctggaaa cgctggataa actgcgcgaa aagtccctct cagggcgcat
    18061 tgtggtattc agcgtctcta accatgaaga agatgtggtc accgcactga aacgcggcgc
    18121 ggatggttat ctgttaaaag atatggaacc ggaagatctg ctgaaagcat tgcatcaggc
    18181 agctgctggc gaaatggtat taagcgaagc attaacgcct gttctggccg ccagcttgcg
    18241 cgctaaccgt gccactactg agcgcgatgt taaccagtta accccacgcg agcgcgatat
    18301 tctcaagctg attgcccagg gtttgccgaa caagatgatt gcccgccgcc tggatatcac
    18361 cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag aaaatgaagc tcaagtctcg
    18421 cgtggaagca gcggtatggg tgcatcagga gcgcattttc tgattacggt tcccagcgca
    18481 gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt tagcgtgatc tcattggagg
    18541 agacacgctg cccctggtta tcttccacca ccaccgacaa tcgccagtga ttgctcgccc
    18601 cttccccgtt ctgccagtca ggcatgatca gcgtccagcc ctccgcgctg ttggcttgtg
    18661 cgcccggcgt caaacttaat atctgcgtat cgccctgcca aatcagttgc cgaataccgt
    18721 aacgactgcg gatttgtaat ttcagcggca ctgtttcgcc aggttttaga tcccacggcg
    18781 gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc ggtagattat
    18841 ttcgctgcgg attatcatag cgactaccac gtaacgactg actttcggca acctcgcccg
    18901 cagaaagttg ttttttgagc ggcacaccaa agcggtaatt aagattcagt ccgaggttat
    18961 tttgattttc gccactttca ccctgtttat gctgggccgt cacagtgact aatggcacag
    19021 gggtgtaatt taatcccaga ctcaacgcga cgggattgtg ataacccgta ccagagttaa
    19081 acaaatcaac acgctcacca aaatactgtt ctaccctgac actggtattg aggtgttgat
    19141 agaacggcat gcgcatccgg gctgtcaggt catacccgcg cgccatccgt tgttcctgcg
    19201 tggctgtctg ttcatgccat gcagcaaacg gctgataaaa gtttgccgat agtcgcaaat
    19261 attcgcccca cgcttcggca ccaaagcccg ctcgctgaag attttcgtcc agcaagttgt
    19321 cataaaaagt gttataaccc accagccagt tgccgcgcgc ccagcgttgc ccaacgccca
    19381 cattgctcac caacccatca tcctgctgag taagaccaag ctggctccag gtgagataac
    19441 gatcattatc ttgtaacggc acaaaccagc ttccacgact gccggtgaaa tgtccttcgt
    19501 tatccacttt gacgtcaaca ctggcatttc cccacggtga tagccaggac tctacgtgct
    19561 gattaacctg ttgactaagc gcgtcgcgga cttttcccaa tgcgaaagct tttgcctgct
    19621 cgccagtatc cagcccgtta tcattcatac tggtttcgcc gaaatctttc acaatttcag
    19681 caaagtgttt ttccccatca tggttttcgg gagccatgcc taaatccggc agaccatcat
    19741 tgttattatc aaagggattt gccgcttttt gctcgaaggt agattgtgcg ttagctgtac
    19801 cgcctgccgc cagcaagagt aaataaaacg ggataatgcg aggaacgaaa cggctcaaaa
    19861 cgtcggtaac ttccggtaat aatgaaaaac catagcctta aagaataacg attactggca
    19921 acttttgtcg tattttctcg cgctttcagg aaaatcctta aaaaggtgac cgattggtca
    19981 gacgctgcag aagcagtaag atagaagatg agtgtctaat tccctgaata caggagtaaa
    20041 taatgcaaaa aatcgtgatc gttgccaatg gcgcacctta cgggagcgaa tccttgttta
    20101 acagcttgcg gctggccatt gcgttacgag agcaggagag caatctggat ctgcgtctgt
    20161 tcctgatgtc tgatgcggtc acagccgggt tgcgcgggca aaaaccaggg gaaggctaca
    20221 acattcagca aatgctggag atccttaccg ctcagaatgt accggtgaaa ttgtgcaaaa
    20281 cctgtaccga cgggcgcggg attagcacac ttcctcttat tgatggggtg gaaatcggta
    20341 ctcttgtgga actggcgcaa tggacgctgt cagccgataa agtgctcaca ttttaatgtt
    20401 tgagggggcg tctgacgccc cactatgagg gaagtttact caggcgaatc ccggccgtaa
    20461 cacaccatcc ggataattct ccgccagcag tttttttacc gataccagca aatcattcag
    20521 cccatcgtcc tgcattccca gtttgataag ctcctgttcc agtgaaaaca ggtattgtgc
    20581 gttggttccc agcggaccgc tcgccgccgc aatcaacggc gcgatgacct gagcgcgagt
    20641 gtcagattca tattctggat gtcgcgggtc cataataaac acaatggcgt ttactgtgcg
    20701 tccatcatca agatcaagct gacaccaggt tggcagataa cagccggtaa tcatctcgcg
    20761 cttccacaac agggttagtt cctgctccag cgtctcttct ggtagtcgat aggcgacgcc
    20821 tgtggtgcgt ccgccctctt tcagtgcaag catccgtccc ggctggtgcg cagtcccgcg
    20881 cccggcggtc aggcgcaggc aaaacgcgcg atgccatcca accagtgtac cggtgcataa
    20941 ttcggtaaac tccagtgccg gattccacat caacgatcca taaccgaaga tccacaccgg
    21001 tccctcatta ggtcgacaag ccagcgtcgc cgccagcgaa gccgcccgtt gttctgctga
    21061 ccataagagt gattcttcaa tggcaccaaa tgccgtttta caatcggcat tcatcaagaa
    21121 atcacgcgtt atcaccttac acctccgcca ctgcatactt ccctgcgaca actttttggt
    21181 gtcttccgga catttttacc gctcattttg aaaccactaa cgggacaata tgcaatccac
    21241 ggaccacttg caaggcaaca ccgcacgaat agctttaact aagctaccgg ttttacgatt
    21301 ttttatgcca tttatcatca tcccctttgg catattcatg cttcacagca gcccaggcca
    21361 ctttatgcgc ggtttcttcg cgactggcgt catcacgccg atcttcttta tctttatatt
    21421 gatcccatgc gctgttgaac gcttctttat agatatcctg ggcatgagac ggtagaacgt
    21481 gctttacgct ttccggcaga tcgcttttcg ttttatacgg catcgttgac tccttttaca
    21541 aagttgaaca ttaagtgtgg tcaacaatat ctactcttgc caaaacataa gaattttcat
    21601 gtcagactgt tcttaagaaa cgctaaaaca aacattgtct aaacgtaata cttattttta
    21661 tgccccaatg agtaaaaaat cgacagcggg gtaaaaattc gtaaaatgtt accgatatcc
    21721 agacactttc tgttattttt cctacctatt ttttacattt tcgataaatt catctgcaaa
    21781 gggataacca taatgtcaaa tgctcaagag gcggtaaaaa cccgccacaa ggagacttcg
    21841 cttattttcc cggttctggc gctggtagtg ctgttcctgt ggggaagcag ccagacacta
    21901 ccagtggtca ttgccatcaa tcttcttgcg cttattggta ttttaagtag cgcctttagt
    21961 gttgtccgtc atgcggacgt attagcccat cgcctgggag aaccttacgg ttcgcttatt
    22021 cttagccttt cagtggttat tcttgaagtc agtttgattt cagctttaat ggcaaccggc
    22081 gacgccgcgc caacgctaat gcgtgatacg ctctattcaa tcattatgat tgttaccggt
    22141 gggctggttg gcttttcatt attgttgggc ggtcgtaagt ttgccaccca atatatgaat
    22201 ctgtttggta tcaagcagta tttaattgcg ctgttccccc tggcgataat cgtactggta
    22261 tttccaatgg ctctgcctgc ggcgaatttt tcaaccggtc aggcgttact ggtagcatta
    22321 atttctgcgg caatgtatgg cgtatttttg ctgatccaga ccaaaacgca tcaaagtttg
    22381 tttgtctacg agcacgaaga tgacagtgat gacgacgacc cgcaccacgg taaaccgtct
    22441 gcccatagca gcgtgtggca tgctatctgg ttgattatcc atctgattgc cgttattgca
    22501 gtcaccaaaa tgaatgccag cccgctggag acattgctcg acagcatgaa tgcccctgtc
    22561 gcctttactg gcttcctggt ggctctgttg attctgtcgc cggaaggttt aggtgcgtta
    22621 aaagcagtgt tgaacaacca ggtccagcgc gcaatgaatc tgttctttgg ttcagtgtta
    22681 gcaaccattt cgcttaccgt acctgtcgtc acgctaattg cctttatgac gggtaacgaa
    22741 ttacagtttg cacttggtgc gccagaaatg gtggtgatgg tggcctcttt agtgctgtgc
    22801 catatctcct tctccaccgg acgtactaac gtgctcaatg gcgcagcgca tctggcactg
    22861 tttgccgcct atttgatgac gatatttgcc tgacagttat atctcctccg atgacggaaa
    22921 tcgggggaga taagataaat tcctgcttat tgtatttctt catactacct ggacagctcg
    22981 ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa ccactgcaac agctgttgaa
    23041 aagccggaat gtactcttta ttgcaaacat ttcagggatg aagtgaagat gaattgagac
    23101 gtagtatcca caccaagccg gaattatccg gtgaggcgca atgttgcggg ggctttatcc
    23161 ctggtggcat tggttgctgg aaagagaaaa cccccgcacg ttgagaggta aaacctgaca
    23221 acaaaacggg ggctaatctt gaaaccagac acttcaagaa tagccgcctt gttgtcatta
    23281 caacaaggcg gctatatgac gctcgcgcag tttgccatga ctttctggca cgacctggca
    23341 gcaccgatcc tggcggggat tattaccgca gcgattgtca gctggtggcg taaccggaag
    23401 taaggtgaaa acgggcgtca ggctgccgtg atggcaatat gcgcccgaaa ccggccgcag
    23461 gggtaacagc ctgcggccat ttttgtcttt atctcttcca caagtaaata ttggcaaccc
    23521 gagctggaag catcatccag agcactcatg aaacgactgt aacagctgtt gaaaaaccgg
    23581 aatgcactct tcattgcaaa catttcaggg atgaagtgaa gatgaattga gtcgtagtat
    23641 ccacaccaag ccggcgttat ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg
    23701 cattggttgc tggaaagaga aaacccccgc acgttgagag gtaaaacctg acaacagaac
    23761 gggggctaat cttgaaccat caacaattca agaatagccg caaaatgttg tcattacaac
    23821 aaggcggcta tatgacgctc gcgaagtttg ccatgatttt ctggcacgac ctggcagcac
    23881 cgatcctggc ggggattatt accgcagcga ttgtcggctg gtggcgtaac cggaagtaag
    23941 gtgaaaacgg gcgtcaggct gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt
    24001 aacagcctgc ggccattttt gtctttatct cctccacaag taaatattgg caacccgagc
    24061 tggaagcatc atccagagca ctcatgaaac gactgtaaca gctgttgaaa aaccggaatg
    24121 cactcttcat tgcaaacatt tcagggatga agtgaagatg aattgagacg tagtatccac
    24181 atcaagccgg cgttatccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt
    24241 ggttgctgga aagagaaaac ccccgcacgt tgagaggttt aacctgacaa cagaacgggg
    24301 gctaatcttg aaccagaaca attcaagaat agccgcaaaa tgttgtcatt acaacaaggc
    24361 ggctatatga cgctcgcgca gtttgccatg attttctggc acgacctggc agcaccgatc
    24421 ctggcgggaa ttattaccgc agcgattgtc ggctggtggc gtaaccggaa gtaaggtgaa
    24481 aacgggcgtc aggctgccgt gatggcaata tgcgcccgaa accggccgca ggggtaacag
    24541 cctgcggcct tttttgtatc tggcacccat gaaaaaagtc ttaacgcaga acgctaaggc
    24601 tttatttttc aagcaaaaaa gattacttgc tggtatccag ttcttcgaaa cctttcacca
    24661 gatcatcaat cgctttcatc tgcttgagga acggttccag tttagccagc ggcagcgcgg
    24721 atggaccatc acatttcgca tgttccggat ccggatgcgc ttcaataaac agccccgcca
    24781 gacctaccgc cataccggct cgtgccagct cagccacctg agcacgacga ccaccggaag
    24841 ctgcgccaaa cggatcgcgg cattgcagtg cgtgggtcac gtcgaaaatc accggcgagt
    24901 taccagacac tttcttcata atgctgaagc ccagcatatc gacaaccagg ttgtcatagc
    24961 cgaagttagc accgcgatcg caaagaatca ctttttcgtt gccgccttct ttgaatttat
    25021 caacgatatt acccatctgt cccgggctga caaactgtgg tttcttgacg ttaattaccg
    25081 caccggtttt cgccatggct tcaaccaggt cagtctggcg agcaagaaac gccggcaact
    25141 gaatcacatc cacgacatca gcaacgggct gtgcctgact tggttcgtga acgtcggtga
    25201 taattttcac gccaaaagtc tgcttcaact cctggaagat tttcatccct tcttccaggc
    25261 ccggtccacg ataagagtgg atggaggagc ggttggcttt gtcaaaagag gctttgaaca
    25321 cgtaagggat acccagtttc tgggtcacgg ttacgtagtg ctcgcaaatg cgcatcgcca
    25381 gatcgcgaga ttccagcacg ttcataccac caaacagtac gaacggcagg tcatttgcta
    25441 cgttgatgtc gccaatgcta accacttttt gtttcatagg atcgccttaa tcttgagtaa
    25501 aatgtcgatt aattaatgca gcacaatatg tttatgcgcg atgttattta tttgcgcacg
    25561 gatcatttcg ctgatcgggt cttccggaca ctgttcaacg aaataactta aatcgttcaa
    25621 cgcaacgtgt tcgcaatcca gttgcgcata aatcaacccg cgatcgcgaa tttcataggg
    25681 atcttccggg ttgaattgta ataaagcttc gctggtgcgt aacgccagct ccatctgatt
    25741 ttcttccatc aacgaggctt tgagtgtatc caacaatttg cggattacct caatgttatc
    25801 agcttcatca aggtcttcat aaaacagttc cgccgacggg ctgatatttc cctttaacca
    25861 tacgtccagc atatgttcgc ttaacgattc accgttaaaa ggattaatca gccaaatttc
    25921 gccatccgga cattcaatgc gcaatatcag ctgcgtaggg aaaatcaccg gcagcagcgg
    25981 caaatcgaga cgattcgcga cccataataa aaccgcacct aatgataccg cactgcctgt
    26041 cgattcttta acacctggtc cagccataat gcatcggaaa gacgataaac gccgcgtgag
    26101 gctttaaatc cccagtcgcc gtaaaacagc gcgatcagtt tttccagctg ctcttctaaa
    26161 ggcagaagct ggctgatttc ttccttcgcc agactaacga gacgctccag ttcgtcgtaa
    26221 acatcttgcg agggaaaatc gcggcggatt gcttcgcaag ccaggatcat gccttcgcac
    26281 aatggcgctt tattaaattc gaaatcagct aacgatctca tgacttaccc cagtaacggt
    26341 acttttgtgg tggcgagttt aatgatgatg tacagcacca ccaacgccag cgggaaggca
    26401 ataatgcgcg cctgctgact gtgcatacga cgataatcga gtgcaataaa acccaaaacg
    26461 atataaatga taactccaaa cagcttttca gtcagccact gtgcctgccc ggaaaatggc
    26521 aggatgtgcg ctttagccat caacgcaatg ccgcttaaca ataacaccgt atcgacaacc
    26581 ggcggcacaa tgcgcgtcca gcgagcaaat gcctgtggat gcttctgata acgtagccag
    26641 aaacgtaagg ttaatagccc aacagaaagc gcgatactaa taagatgaac actaagcagt
    26701 gtagaaaaac ttgtcattga taatagcggc cgagcgttac gcgctcgtta tcaccatagt
    26761 cacggcaggt ttcgacgtca tgataccccg cgaggataaa tgcttgtcgc accgcttcgc
    26821 cctgctgcca gccatgttcc agaagcagaa agccgccgga taccagcgcg ttacgcgact
    26881 gttcgatgat atgcacgatg tctgccattc cactgtctgc cgcaaccagc gcagtgagcg
    26941 gctcaaagcg gacatcgcct tgttgaagat gtgggtcctg ctcgtcaata tacggcggat
    27001 tgctgacaat catcgcaaac tgctgcccgg ctagcgcgct aaaccagtcg ctttgcagaa
    27061 tgtggatatt tttgatcgcc agatgctggg cattacgttg tgccagggag acagcatcag
    27121 gcatacgatc tacagcgata atttcgcagt ccgggcgctc gctagccagc gcaagcgcaa
    27181 tcgccccggt acccgtcccg agatcgagaa tacggcaagg ttgttcaggc aaccgcgcca
    27241 gtgcctgctc caccagacac tccgtatccg ggcgcggaat taaggtcgct ggcgaaacaa
    27301 ataacggcaa cgaccagaat tctcgcaccc cggttaaatg agcaatgggt tcaccatcgc
    27361 gacgacgtgt cagtagcgca tcaagttgct gacattgttc gtcagtcagc tgcgtttcac
    27421 caaaggccag aataaaagtc cgccccctgc cagtgacata ctccaataag atttcagcgt
    27481 cacggcgtgg gctttcgctc gcctgaagtt ggcttattgc ttcacgtaac cagtattgat
    27541 attccattat tcctgctcgg acaacgccgc cagttggtcg gcctgatgtt cctggataat
    27601 cggttcaatc agcatatcca gcttaccttc catcacttca tccagacggt agagcgtcag
    27661 gttgatgcgg tgatcggtaa cgcgcccctg cgggaagttg taagtacggt tacggtcgct
    27721 gcgatcgcca ctccccagca ggttacgacg ggtagacgct tcggcctgtt gccgttttgc
    27781 catttctgca gcgtggatac gagcaccaag tacagaaagt gctttagctt tgtttttatg
    27841 ttgtgaacgt tcgtcctgac attcaacaac aatcccggtc ggcaagtgag taatacgaat
    27901 tgccgaatcg gtggtgttaa cgtgctgacc acccgcccct gacgagcgga aagtatcaat
    27961 gcgtaaatct gctgggttga tgtccggcag ttctgcgtca ggcagttctg gcattaccgc
    28021 aacggtacaa gcagaagtat gaatacgacc ctgcgattcc gtagcaggaa cacgctgcac
    28081 gcgatgaccg ccggattcaa atttcagacg accatacaca ccatcaccgc taattttggc
    28141 gatgatctct ttataaccac catgttcacc ctcgctggcg ctcatgattt ctacccgcca
    28201 gcggcgggct tcggcataac ggctgtacat acggaacaga tcgcccgcga acagcgccgc
    28261 ttcgtcgccg ccggttccgg ctcggacttc gaggaaggcg ttacgttcgt catcaggatc
    28321 ttttggcagt aacagaacct gtaattgctg ttccagttgc tcgctttttt ctttagcttc
    28381 gcgcagttca tcctgcgcca tctcacgcat ttcaggatca tcgagcatca tctgtgcggt
    28441 ttcgatatct tcctgaacct gttgccagtc ggtaaaacag cgcgaaacat cacttaactg
    28501 cgcatattcg cgtgataatg cgcgaaaacg ttcctggtcg gcgatagttt gcgcgtcacc
    28561 cagcaacgcc tgaacttctt catggcgttc atgcagggct tccagtttgg caacgataga
    28621 aggcttcata ggcgtaaatg caccctgtaa aaaaagaaaa tgatgtactg ctactccagc
    28681 ccgaggctgt cgcgcagaat attcaggcgt tcgttatccc cgtcacgggc ggcctgttga
    28741 agtgatttcg ttggcgcatg gatcaagcgg ttagtcagtt tccatgccag atcctgcata
    28801 atggcttgcg cgtcgccgcc ctgctcaagg gccgctaacg ctttggcggt taactcatcg
    28861 cgaacgtgct ctgcctggct gcgatactcg cgaatggttt cgctggcgct ttgtgctcgc
    28921 agccacgcca taaattcgct ggtttcctga gcgacaatag tttccgcctc aaccgctgcg
    28981 gctttacgct gcgccaggtt gtgcgaaatg atgctttgca gatcgtccac actataaaga
    29041 taagcattcg ccagtttgcc aacttccggc tcaacatcgc gcggaacggc aatatccacc
    29101 aacagcattg gttggttacg acgacttttt aatgcgcgct ccaccatgcc tttcccgata
    29161 atcggtaacg ggctggcggt ggaactgatg atgatatcgg cttcgcgcag acgttcgtcg
    29221 atctcactca gggcaatcac ttccgcgcca acttcatctg ccagaatttg ggcacgttcg
    29281 cgagtgcggt tggcgataat catcttctgt actttatgtt cgcgcagatg acgcgctacc
    29341 agctcgatgg tttcgcccgc gcctaccagc aacactgtga ccgtagagag cgattcaaag
    29401 atctgccgcg ccagcgtaca agccgcaaaa gcgacagaca cagcactggc accgatatct
    29461 gtttcagtgc gaacgcgttt cgctacagag aaagatttct ggaacatgcg ttccagttcg
    29521 ctggccttca tatgaccttt ttgcgaatcg gcaaacgctt ttttaacctg accgaggatc
    29581 tgcggctccc caagaaccag tgaatccagg ccgctggcaa cacgcattaa atggctaacc
    29641 gcgtcgttat cctgatgcca gtagaggctt ttacgcagat cttcttcatt aagattgtga
    29701 taatcgcaaa gccagcggat taacgcctct tgcaggttat cctgctcttc aacgctaaga
    29761 taaagttccg tgcggttgca cgtcgacagc accacgccgc cctgcaccat cggctgcgca
    29821 agcaggctgt caagcgcctg atcgagctta tccggcgaaa acgatacacg ttctcgcagc
    29881 gatacaggtg ccgttttatg gttgataccg agtgctaaaa gggtcatgtc tgcgggaaat
    29941 aataccaacg ttgatatggt tagtctgctt gcatcataca ggatgcgtaa gatcaataaa
    30001 agagagcgcc cccttttgga gtaattgccg aaagccgtta gattctggca attaagacaa
    30061 cttgaacata gacgatagcg gacggtaacg ctagcattaa gggttataac tgcaacgtat
    30121 ctcaaggact tgtcatcact atgccccagc ccgattttcg tcttatccgc ctgctaccgc
    30181 tggctgctct tgtgctcact gcctgttccg ttaccacgcc caaaggtcct ggcaaaagcc
    30241 cggattcgcc acaatggcgt cagcatcagc aagacgtgcg caatcttaat cagtatcaga
    30301 ctcgcggcgc gttcgcttat atttctgacc aacaaaaagt gtacgcccgc tttttctggc
    30361 agcaaaccgg ccaggatcgc taccgtctgc tgctcactaa cccattgggc agcacggaac
    30421 tggagctgaa tgctcaaccg ggtaacgtgc agttagtcga caataaaggt cagcgttata
    30481 ccgccgatga cgccgaagag atgattggca aattgaccgg aatgccaatt ccgctcaaca
    30541 gcttgcgcca atggatttta ggtttaccgg gtgatgcaac cgactacaaa ctggacgacc
    30601 agtaccgcct gagcgaaatt acctacagcc agaatggcaa aaactggaag gttgtttatg
    30661 gtggttatga caccaaaacg caacctgcga tgccagccaa tatggaactc accgacggtg
    30721 gtcaacgcat caagttaaaa atggataact ggatagtgaa ataatgcgga cacagtggcc
    30781 ctctccggca aaacttaatc tgtttttata cattaccggt cagcgtgcgg atggttacca
    30841 cacgctgcaa acgctgtttc agtttcttga ttacggcgac accatcagca ttgagcttcg
    30901 tgacgatggg gatattcgtc tgttaacgcc cgttgaaggc gtggaacatg aagataacct
    30961 gatcgttcgc gcagcgcggt tgttgatgaa aactgcggca gacagcgggc gtcttccgac
    31021 gggaagcggt gcgaatatca gcattgacaa gcgtttgccg atgggcggcg gtctcggcgg
    31081 tggttcatcc aatgccgcga cggtcctggt ggcattaaat catctctggc aatgcgggct
    31141 aagcatggat gagctggcgg aaatggggct gacgctgggc gcagatgttc ctgtctttgt
    31201 tcgggggcat gccgcgtttg ccgaaggcgt tggtgaaata ctaacgccgg tggatccgcc
    31261 agagaagtgg tatctggtgg cgcaccctgg tgtaagtatt ccgacaccag tgatttttaa
    31321 agatcctgaa ctcccgcgca atacgccaaa aaggtcaata gaaacgttgc taaaatgtga
    31381 attcagcaat gattgcgagg ttatcgcaag aaaacgtttt cgcgaggttg atgcggtgct
    31441 ttcctggctg ttagaatacg ccccgtcgcg cctgactggg acaggggcct gtgtctttgc
    31501 tgaatttgat acagagtctg aagcccgcca ggtgctagag caagccccgg aatggctcaa
    31561 tggctttgtg gcgaaaggtg ttaatctttc cccattgcac agagccatgc tttaagccgg
    31621 gcaagctgag tttcggtgac aacgtcacct tgttccagac gttgcatcgc gctctttaat
    31681 acaccgcctg gaaaggatca tgcctggccc gcacagtttt cggcagattc tttccaccaa
    31741 tggacgcatg cctgaggttc ttctcgtgcc tgatatgaag ctttttgctg gtaacgccac
    31801 cccggaacta gcacaacgta ttgccaaccg cctgtacact tcactcggcg acgccgctgt
    31861 aggtcgcttt agcgacggcg aagtcagtgt acaaatcaac gaaaatgtac gcggtggtga
    31921 tattttcatc atccagtcca cttgtgcccc tactaacgac aacctgatgg aattagtcgt
    31981 tatggttgat gccctgcgtc gtgcttccgc aggtcgtatc accgctgtta tcccctactt
    32041 tggctatgcg cgccaggacc gtcgcgtccg ttccgctcgt gtaccaatca ctgcgaaagt
    32101 ggttgcagac ttcctctcca gcgtcggtgt tgaccgtgtg ctgacagtgg atctgcacgc
    32161 tgaacagatt cagggtttct tcgacgttcc ggttgataac gtatttggta gcccgatcct
    32221 gctggaagac atgctgcagc tgaatctgga taacccaatt gtggtttctc cggacatcgg
    32281 cggcgttgtg cgtgcccgcg ctatcgctaa gctgctgaac gataccgata tggcaatcat
    32341 cgacaaacgt cgtccgcgtg cgaacgtttc ccaggtgatg catatcatcg gtgacgttgc
    32401 aggtcgtgac tgcgtactgg tcgatgatat gatcgacact ggcggtacgc tgtgtaaagc
    32461 tgctgaagct ctgaaagaac gtggtgctaa acgtgtattt gcgtacgcga ctcacccgat
    32521 cttctctggc aacgcggcga acaacctgcg taactctgta attgatgaag tcgttgtctg
    32581 cgataccatt ccgctgagcg atgaaatcaa atcactgccg aacgtgcgta ctctgaccct
    32641 gtcaggtatg ctggccgaag cgattcgtcg tatcagcaac gaagaatcga tctctgccat
    32701 gttcgaacac taatcgaacc cgctcaaaga cccgctgcgg cgggtttttt tgtctgtaat
    32761 atccatttgt atgacctatg cctccttcac ctgccattta gttgacagat gatgcgctca
    32821 cggatgaaac attattgtga acaaaatatt ttcctcacat gtgatgcctt tccgcgctct
    32881 gatcgacgct tgctggaaag aaaaatatac tgccgcacgg tttacccgtg acctgattgc
    32941 cgggataacc gtcgggatta ttgctatccc gctggcgatg gcgttggcta ttggtagtgg
    33001 tgtggcaccc cagtacggtt tatataccgc agctgttgcg gggattgtca ttgctctgac
    33061 gggtgggtca cgctttagcg tttccggtcc gactgcggca tttgtggtaa ttctctatcc
    33121 cgtgtcgcaa cagtttggac tggcaggact gctggttgcg accttgctgt cggggatctt
    33181 tttgattctg atgggtctgg cacgctttgg tcgcctgatt gagtatattc cggtttccgt
    33241 caccttaggt ttcacctcgg gtatcgggat caccatcggt accatgcaga ttaaagattt
    33301 tctcggtctg caaatggccc atgtcccgga acattatcta caaaaagtcg gcgcattatt
    33361 tatggcgctg ccgaccatta atgtgggtga tgctgccatt ggcattgtga cgctaggtat
    33421 tcttgttttt tggccgcgtc tgggcattcg tttacccggt caccttccgg ccttgctggc
    33481 tggttgcgcg gtgatgggga ttgttaacct gctcggcgga catgttgcta ccatcggttc
    33541 gcaattccac tacgtcctgg ccgatggttc tcagggtaac ggtattccgc aactgctgcc
    33601 gcaactggtg ctgccgtggg atctgcctaa ttcagaattc acgctaacct gggattctat
    33661 tcgcacactg ctgcctgcgg cattctcaat ggcaatgctc ggcgcaatcg aatctctgct
    33721 ctgcgccgtg gtactggatg gtatgaccgg gacgaaacac aaggcgaaca gcgaactggt
    33781 tggacaggga ctggggaata ttatcgctcc gttctttggt ggtattaccg ctacagctgc
    33841 catcgcgcgt tctgccgcta acgtccgtgc cggggcaact tcccctatct cggcggtgat
    33901 ccactctatt ctggttattc ttgccctgct ggtactggca ccgctgctct cctggctgcc
    33961 gctttccgct atggcagccc tgctgttgat ggtggcgtgg aacatgagtg aagcgcataa
    34021 agtggtcgac ttgctgcgtc atgcaccgaa agatgacatc attgtcatgc tgctgtgcat
    34081 gtcgctgacc gtgctgtttg atatggttat tgccatcagc gtggggatcg tgctggcatc
    34141 gctgctgttt atgcgtcgta tcgcacgtat gactcgcctg gcaccggtag tcgtagatgt
    34201 tccagacgat gttctggtac tgcgcgttat tggcccgctg ttttttgctg ctgctgaagg
    34261 cttgttcacg gacctggagt cacgtcttga aggcaaacgg attgtgattc tgaagtggga
    34321 tgccgttccg gtacttgatg ctggtggtct tgatgcgttc cagcgttttg tgaagcgtct
    34381 gcccgaagga tgtgaactgc gcgtgtgcaa cgtggaattc cagccactgc gcactatggc
    34441 tcgcgcaggc attcaaccga tcccgggacg cctcgcgttc ttcccgaatc gtcgcgcggc
    34501 gatggcggat ttataagtac gagattgacc agtcagcgca aactgactgg tcagcaaact
    34561 gcattatttg ttagctatga cggcgattat cgctacggcg gcaacgtttg tcatagtgac
    34621 gaacccacca gtaacggtcg cacacctgtt catgcccgcc aacacgggca cccgccagcc
    34681 agagaatggc accgacgaaa atacttagca ctgcaccatg tgcgaaatac tggggcatat
    34741 taaactgtgg taactggttg aggattgaat accccacgcc gaccaccatt accaccagcc
    34801 ccaaccccat gagcacgtta ccgagtaacg aagcgttttt gcgtttcata tgtcacctcc
    34861 ggaactttct gggttgtaac agggaatacc cctcttcctt atgtgtaaag tatagacaac
    34921 accctgtggg ttatgtgcgg gcgtgatcac aattacaacc cttatttcaa caaaacttta
    34981 caaataaacg cctgaacacc ttgactttct aatagtccac tcaggcaatt attacgttat
    35041 ctgcatttga tacgcagttt tttgtcaagc ggggccgcaa ccagtaaact acgcgccagt
    35101 tatgtacaca ctcaggacaa aaaacgtgac gattaaattg attgtcggcc tggcgaaccc
    35161 cggtgctgaa tacgccgcaa cgcgacataa tgctggtgcc tggttcgttg acttactggc
    35221 agagcgtttg cgcgctccgc tgcgcgaaga ggctaaattc tttggttata cttcgcgagt
    35281 cactcttgga ggcgaagatg tccgcctgtt agtcccgact acatttatga atctcagcgg
    35341 caaagccgtt gcggcgatgg ccagtttttt ccgcattaat ccggacgaaa ttctggtggc
    35401 ccacgacgaa ctggatctgc ctcctggcgt cgccaaattt aaattgggcg gtggccatgg
    35461 tggtcacaat ggactgaaag acatcatcag taaattgggt aataacccta actttcaccg
    35521 tttacgcatc ggaatcggtc atcctggcga taaaaataaa gttgtcggtt ttgtgttagg
    35581 caaaccgcct gttagtgaac agaagttaat tgatgaagcc atcgacgaag cggcgcgttg
    35641 tactgaaatg tggtttacag atggcttgac caaagcaacg aaccgattgc acgcctttaa
    35701 agcgcaataa gtcgttgtct gcggcatttt tgccgagtgc cgtgtataat aggcaaagtt
    35761 atttccattt ctgcaatctg ttagcaataa caggttgatt attaagatat taaggtgatt
    35821 taaatcatgg gattcaaatg cggtatcgtc ggtttgccca acgtcgggaa atctaccctg
    35881 ttcaacgcgc tgaccaaagc cggtattgaa gcggccaact ttccattctg caccattgag
    35941 ccgaacacag gcgtcgtacc aatgcctgac cctcgcctgg atcaactggc tgaaatcgta
    36001 aaaccgcaac gtacgcttcc cacgaccatg gaatttgtcg atatcgccgg tctggtaaaa
    36061 ggcgcatcga aaggcgaagg tctgggtaac cagttcctga ccaacatccg tgaaaccgaa
    36121 gcgatcggtc acgttgttcg ctgctttgaa aatgacaaca tcattcacgt ttccggcaaa
    36181 gttaacccgg ctgacgatat tgaagttatc aacaccgaac tggcgctggc agacctcgac
    36241 acctgcgaac gtgcgattca tcgcgtacag aagaaagcca aaggtggcga taaagacgcg
    36301 aaagctgagc tggcggtcct agaaaaatgc ctgccccagc tggaaaacgc aggtatgctg
    36361 cgcgcgctgg atttaagcgc tgaagagaaa gcggctattc gttacctgag cttcctgacg
    36421 ctgaaaccaa caatgtacat cgccaacgtc aacgaagacg gttttgaaaa caacccatat
    36481 cttgaccagg tgcgtgaaat cgcggcgaaa gaaggttctg ttgtggttcc ggtttgtgct
    36541 gctgttgaag cagacattgc cgaactggac gacgaagaac gtgacgagtt tatgcaggag
    36601 cttgggctgg aagaaccggg cctgaaccgt gtgatccgtg tcggttataa gctgctgaac
    36661 ctgcaaactt acttcaccgc tggggtgaaa gaagtgcgtg catggaccat tccggttgga
    36721 gcaaccgcgc cgcaggcagc gggcaaaatc catactgatt ttgaaaaagg ctttatccgt
    36781 gcacaaacca tctcgtttga agatttcatc acttacaaag gtgaacaagg cgcgaaagaa
    36841 gcaggcaaaa tgcgtgcaga aggtaaagat tacatcgtta aagatggcga tgtgatgaac
    36901 ttccttttca acgtctaatt aatttattgt ctcatgaggt ttcgctaaat ctcatgaaga
    36961 gcgcaaaagt atataaaatc cacgcaattg cgtggatttt tcatttcacc gagtcctaat
    37021 cattcttgta acatcctaca cttttgtgct tattgcctac agtgaaccac gagaataatt
    37081 aaagacatca gtgtcagatg acggatatct cattagtagt gatataacgt aagtttttgt
    37141 tcaactacac atcaccccct tcacagctga tcaatattga tcacgctcca atatgctggc
    37201 gagaaaagga cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt
    37261 acgtgcgatc ctggcaagat gaaaatgata accagtagca caataacaac gataggccag
    37321 aaaacccatt gaccaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa
    37381 aaatggcgca tattgttttt gttatgcaga tacatcaagc gaaattccca taactaatta
    37441 ggattattac aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc
    37501 catatttata gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa
    37561 ctcacctttg taagggggat gttaaatata tattaattcc tgtgttcctg attttgcccg
    37621 gtaatagaga attattctct gagaagctca tcatgattgc accatacaaa tatcatgatg
    37681 ataagaggat attattcatt gatttaaaca aaatgacaaa atccatagac ccatagtctg
    37741 ggtactatat aggagtggta tgggcatcaa acaacacaat gggaatacca aagcagatcg
    37801 tctcgctgaa t
//
LOCUS       DAZOXR010000016        37768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZOXR010000016 DAZOXR010000000
VERSION     DAZOXR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.016"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHUNN_000714"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHUNN_000714"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0575177.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUNN_000715"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUNN_000715"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0575178.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUNN_000716"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUNN_000716"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0575179.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUNN_000717"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUNN_000717"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0575180.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUNN_000718"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUNN_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0575181.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUNN_000719"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUNN_000719"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0575182.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUNN_000720"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUNN_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0575183.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUNN_000721"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUNN_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0575184.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHUNN_000722"
     CDS             6537..6773
                     /locus_tag="ACHUNN_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0575185.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHUNN_000723"
     CDS             6776..7633
                     /locus_tag="ACHUNN_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0575186.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHUNN_000724"
     CDS             7654..9645
                     /locus_tag="ACHUNN_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0575187.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUNN_000725"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUNN_000725"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0575188.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHUNN_000726"
     CDS             complement(10248..11144)
                     /locus_tag="ACHUNN_000726"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0575189.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHUNN_000727"
     CDS             complement(11193..11432)
                     /locus_tag="ACHUNN_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0575190.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUNN_000728"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUNN_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0575191.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUNN_000729"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUNN_000729"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0575192.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUNN_000730"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUNN_000730"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0575193.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUNN_000731"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUNN_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0575194.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHUNN_000732"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHUNN_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUNN_000733"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUNN_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0575195.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUNN_000734"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUNN_000734"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0575196.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUNN_000735"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUNN_000735"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0575197.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHUNN_000736"
     CDS             complement(21570..22739)
                     /locus_tag="ACHUNN_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575198.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUNN_000737"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUNN_000737"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0575199.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHUNN_000738"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHUNN_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0575200.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUNN_000739"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUNN_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0575201.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHUNN_000740"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHUNN_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0575202.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUNN_000741"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUNN_000741"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0575203.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUNN_000742"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUNN_000742"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0575204.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUNN_000743"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUNN_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0575205.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHUNN_000744"
     CDS             complement(30320..31576)
                     /locus_tag="ACHUNN_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0575206.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHUNN_000745"
     tRNA            31884..31960
                     /locus_tag="ACHUNN_000745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHUNN_000746"
     tRNA            31965..32041
                     /locus_tag="ACHUNN_000746"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHUNN_000747"
     CDS             32149..32454
                     /locus_tag="ACHUNN_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0575207.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHUNN_000748"
     CDS             32580..34184
                     /locus_tag="ACHUNN_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR0575208.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUNN_000749"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUNN_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0575209.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUNN_000750"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUNN_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0575210.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHUNN_000751"
     CDS             complement(35794..36462)
                     /locus_tag="ACHUNN_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0575211.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHUNN_000752"
     CDS             complement(36526..37164)
                     /locus_tag="ACHUNN_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0575212.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..>37768)
                     /locus_tag="ACHUNN_000753"
     CDS             complement(37177..>37768)
                     /locus_tag="ACHUNN_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HFR0575213.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9023 a         9930 c         9380 g         9435 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaacca cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccg
//
LOCUS       DAZOXR010000017        36830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZOXR010000017 DAZOXR010000000
VERSION     DAZOXR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.017"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUNN_000754"
     CDS             complement(<1..215)
                     /locus_tag="ACHUNN_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575214.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSR"
     gene            complement(315..1226)
                     /gene="gsiD"
                     /locus_tag="ACHUNN_000755"
     CDS             complement(315..1226)
                     /gene="gsiD"
                     /locus_tag="ACHUNN_000755"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0575215.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1229..2149)
                     /gene="gsiC"
                     /locus_tag="ACHUNN_000756"
     CDS             complement(1229..2149)
                     /gene="gsiC"
                     /locus_tag="ACHUNN_000756"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0575216.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2167..3705)
                     /gene="gsiB"
                     /locus_tag="ACHUNN_000757"
     CDS             complement(2167..3705)
                     /gene="gsiB"
                     /locus_tag="ACHUNN_000757"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0575217.1"
                     /translation="MAKAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3725..5596)
                     /gene="gsiA"
                     /locus_tag="ACHUNN_000758"
     CDS             complement(3725..5596)
                     /gene="gsiA"
                     /locus_tag="ACHUNN_000758"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0575218.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5583..6548)
                     /gene="iaaA"
                     /locus_tag="ACHUNN_000759"
     CDS             complement(5583..6548)
                     /gene="iaaA"
                     /locus_tag="ACHUNN_000759"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0575219.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6752..7987
                     /gene="moeA"
                     /locus_tag="ACHUNN_000760"
     CDS             6752..7987
                     /gene="moeA"
                     /locus_tag="ACHUNN_000760"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0575220.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7987..8736
                     /gene="moeB"
                     /locus_tag="ACHUNN_000761"
     CDS             7987..8736
                     /gene="moeB"
                     /locus_tag="ACHUNN_000761"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0575221.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8915..9577)
                     /gene="fsa"
                     /locus_tag="ACHUNN_000762"
     CDS             complement(8915..9577)
                     /gene="fsa"
                     /locus_tag="ACHUNN_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0575222.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9708..10607
                     /locus_tag="ACHUNN_000763"
     CDS             9708..10607
                     /locus_tag="ACHUNN_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0575223.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10613..13045
                     /locus_tag="ACHUNN_000764"
     CDS             10613..13045
                     /locus_tag="ACHUNN_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0575224.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13191..14006
                     /gene="ybiV"
                     /locus_tag="ACHUNN_000765"
     CDS             13191..14006
                     /gene="ybiV"
                     /locus_tag="ACHUNN_000765"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0575225.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14158..15423
                     /locus_tag="ACHUNN_000766"
     CDS             14158..15423
                     /locus_tag="ACHUNN_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0575226.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15390..16550)
                     /locus_tag="ACHUNN_000767"
     CDS             complement(15390..16550)
                     /locus_tag="ACHUNN_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0575227.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16788..18089
                     /locus_tag="ACHUNN_000768"
     CDS             16788..18089
                     /locus_tag="ACHUNN_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575228.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18104..20467
                     /locus_tag="ACHUNN_000769"
     CDS             18104..20467
                     /locus_tag="ACHUNN_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR0575229.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20647..22242)
                     /locus_tag="ACHUNN_000770"
     CDS             complement(20647..22242)
                     /locus_tag="ACHUNN_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0575230.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22461..23381
                     /gene="ldtB"
                     /locus_tag="ACHUNN_000771"
     CDS             22461..23381
                     /gene="ldtB"
                     /locus_tag="ACHUNN_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0575231.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23440..24558)
                     /locus_tag="ACHUNN_000772"
     CDS             complement(23440..24558)
                     /locus_tag="ACHUNN_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0575232.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24555..25022)
                     /gene="mntR"
                     /locus_tag="ACHUNN_000773"
     CDS             complement(24555..25022)
                     /gene="mntR"
                     /locus_tag="ACHUNN_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0575233.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25208..25336
                     /gene="mntS"
                     /locus_tag="ACHUNN_000774"
     CDS             25208..25336
                     /gene="mntS"
                     /locus_tag="ACHUNN_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0575234.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25608..27191
                     /gene="opgE"
                     /locus_tag="ACHUNN_000775"
     CDS             25608..27191
                     /gene="opgE"
                     /locus_tag="ACHUNN_000775"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0575235.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27240..27755)
                     /gene="ompX"
                     /locus_tag="ACHUNN_000776"
     CDS             complement(27240..27755)
                     /gene="ompX"
                     /locus_tag="ACHUNN_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0575236.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27808..27873)
                     /locus_tag="ACHUNN_000777"
     CDS             complement(27808..27873)
                     /locus_tag="ACHUNN_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575237.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28108..28995
                     /gene="rhtA"
                     /locus_tag="ACHUNN_000778"
     CDS             28108..28995
                     /gene="rhtA"
                     /locus_tag="ACHUNN_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0575238.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29294..29797
                     /gene="dps"
                     /locus_tag="ACHUNN_000779"
                     /gene_synonym="pexB"
     CDS             29294..29797
                     /gene="dps"
                     /locus_tag="ACHUNN_000779"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0575239.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30201..30947
                     /gene="glnH"
                     /locus_tag="ACHUNN_000780"
     CDS             30201..30947
                     /gene="glnH"
                     /locus_tag="ACHUNN_000780"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0575240.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31086..31745
                     /gene="glnP"
                     /locus_tag="ACHUNN_000781"
     CDS             31086..31745
                     /gene="glnP"
                     /locus_tag="ACHUNN_000781"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0575241.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            31742..32464
                     /gene="glnQ"
                     /locus_tag="ACHUNN_000782"
     CDS             31742..32464
                     /gene="glnQ"
                     /locus_tag="ACHUNN_000782"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0575242.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            32581..34806
                     /gene="ybiO"
                     /locus_tag="ACHUNN_000783"
     CDS             32581..34806
                     /gene="ybiO"
                     /locus_tag="ACHUNN_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR0575243.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(34803..35729)
                     /gene="rlmF"
                     /locus_tag="ACHUNN_000784"
     CDS             complement(34803..35729)
                     /gene="rlmF"
                     /locus_tag="ACHUNN_000784"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0575244.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36005..36265
                     /gene="mcbA"
                     /locus_tag="ACHUNN_000785"
     CDS             36005..36265
                     /gene="mcbA"
                     /locus_tag="ACHUNN_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0575245.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36530..>36830
                     /locus_tag="ACHUNN_000786"
     CDS             36530..>36830
                     /locus_tag="ACHUNN_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0575246.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         8905 a         9963 c         9259 g         8703 t
ORIGIN      
        1 cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat gatgaaaaaa
       61 agaccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat ctttatcttt
      121 tcgtataaac tcagcatgtt aatacattta acaaaataac tatctgaacg ctgtgccgct
      181 gcaaaaatag tcccttgctg cggcttttta accaaattac ccgattctac aaaatcagca
      241 gaaagttaca acacttgctt agggcaataa tttcctgtga ggtgattacc ctttcaagca
      301 atattcaaac gtaactatcc tttaattttc ggatccagcg catcgcgtaa accatcgccc
      361 aacaaattga acgccagtac ggtcagaaaa atagccaggg ccggaaaaac agcgacatgc
      421 ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg ctccccactc tggtgtcggc
      481 ggctgcgcac cgaggccgag aaatgacagg ctggcggcag agataatcga ggtgccaatg
      541 cgcatggtga aaaacaccac gatagacgag acggtcccag gcaagatatg acgcaataaa
      601 atggtcatat cgctggcacc aatactgcgt gctgactcaa taaaggtttg ctgtttcaac
      661 accagcgtgt tgccgcgcac cagtcgggca aacgcgggga tggaaaaaat ggcgactgcg
      721 ataatcacgt tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc cagtaaaata
      781 cccgggaagg caaacagcac atcgcaaatg cgcatgatca gccgatccca ccagccttca
      841 taatatccag ccaacaagcc cagcaacgtc ccgatcgccg caccgataaa cacggcaaac
      901 acgcccgccg ccagcgagat ttgcgcacca accaggacac ggctgaaaat gtcgcgcccc
      961 agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt gtcataatca
     1021 aaataatttt cggcgtcata gggagcgatc cagcgtgcaa aaatggccac cacaatcaat
     1081 aaaataacga ataatgcggc ggtcatcgcc atatgctgac ggcgaaatcg tcgccagaat
     1141 tcatgccacg gtgtacgcac ctggtcaggt ttgaccagtg gcatggcgtt taacaccgcc
     1201 tgacgtcgcc agttaaatag tcgcatcctt acttgtacct gatagccggg ttaatggcgg
     1261 cgtaaagcac atccaccact aagttgataa gaataaattc cagcgagaaa agcagaattt
     1321 ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc aacgagtaag cgtccaagtc
     1381 cgggccagtt gaaaactttc tccaccacga tagaaccacc gagcaaaaag ccaaactgta
     1441 agcccatcat ggtcactacc gggatcatcg cgttacgtag cccgtgtttg aggacaaccc
     1501 aggtttcgct cacccctttc gccctcgcgg tacgcatata atcttcgctt aaaacatcga
     1561 caaacgacgc gcgggtaaag cgcgccatca cggctgccac tgccgcgccg agcgtcaggg
     1621 agggtaaaat gtagtgctgc caactgtctg ctcccacggt aggcagccag cccagttcaa
     1681 cggagaatac ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag atccccgaca
     1741 ccgcaatggt catactcaat cgatccggcc aacgattacg ccagacggcg gcgataattc
     1801 ccgccgccat accaaatatc accgcccaga ccatactggt tatggtcagc cacagcgttg
     1861 gcataaagcg gctggcaatc tcatcggcaa ccggacgacg cgacaccatc gacaggccaa
     1921 aatccccctg cacagcattg ctgatatagt gccagaactg gtgatacagc ggctgatcca
     1981 accccagctg ctgacgcacc agttctataa cctgcgcatc agcttcgagc ccggcaatca
     2041 atcgcgccgg atcgccgggc agcatatgca caaataaaaa caccagcacc gagacgataa
     2101 acagcgtcgg aatcaacccc agtaagcgtt tgataacgta attaagcatt ccactccctg
     2161 cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca tgatccaaaa
     2221 accggtcagg tttttactgt gtgccgacac cagtttttct accaccagcg ggatccacgg
     2281 cgactcttgc cagatgatat cctgcgccgc cttatacaag cgggtctttt ccgccggatc
     2341 attagttttc agtgcctgag ccaggaagtc gtccacctgt ttattgctgt aaaatgcggt
     2401 attaaacagc gtcggtggcc agttttgcga ggcgaacagt ggcgatagcg cccagtccgc
     2461 ttcgccggtt gaagccgacc agccagtgta gaacatccgc acgccgctct ctttttgccc
     2521 tttaccttca acttctgccg cccgctgtcc ggcatccatc gcggtcacct gggctttaat
     2581 cccgacctgc gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg tgctgtggtt
     2641 atgtgacgac cacagcgtgg tactgaaacc gttgggatat cccgcctctt tcagtaattc
     2701 gcgcgctttc actggatcgt aaggccacgg tttataactt tgcgcgtagg cgatacttgg
     2761 cggtaccaca ccagtagctg gcgttgcata gcccgcaaag gcaactttca ccagcgccgg
     2821 acggttaatg gcgtaattca gcgcctcacg gaccttcggg ttatcgaacg gcttttgcgt
     2881 cacgttcata ctgatataac gctgcataat tgacggactg gccatcaact cgatattttt
     2941 gtttttctcc agcagtgtgg cctgctcgta gggaatgggg aaagcaaact gcgcttcacc
     3001 ggtttgcagc attgccgcgc gggtgttgtt atccgccacc ggacgccagg ttatgctgtc
     3061 cagtttgggc aatcctggct gccagtaacc cgcgaatttt ttcaccttca caaaatcggt
     3121 ctgattccag gtatccagtt catacggtcc ggttcccacc ggatgaaaac caatctcctt
     3181 gccatatttt tccagcgctg ccggtgaaat catcgcggtc gccggatggg caagaatatt
     3241 aataaacgct gagaacggct gtttgagggt aatctttacc gttgtcggat cgatcgcctc
     3301 cgttttggca atattcttat acaggttata gcgtttaaga tgattcgccg gatcgctggc
     3361 ccggtccaga ttcgctttta ccgccactgc gttgaaatcg gtgccatcct ggaatttaat
     3421 gccttcccgc aatttcacgg tgtaagtaat gccgtcatcg gaaacggtat aactctccgc
     3481 cagcacgttt ttcagtttca tctctttatc cagaccgaac agcccctggt aaaacgattt
     3541 cgctacggcc tgagataacg tatcattcgc gtcatacgga tcgagcgtgg tgaaattcga
     3601 tcctaccgcc accaccacat ctttggcagc gaatgcacaa gatgccatca acgctgtcgc
     3661 aatgcccagc gccactaacc cactacggtg tacagctttt gccattttat tctccgcctg
     3721 aatgttatct acgcatgaat gcgtattctg attgtggttg tgcgacgtaa tgccccggcc
     3781 cgacgcattg caacgagacg gctgccacct cttcgccacg cagatgaata ttgctgggaa
     3841 gatcgtccga cagcagtaca cgctgcggtc gttgtcggga cggttcagcg accggaactg
     3901 ccgccagtaa tttacgcgta taaggatgct gcgggttttc gaagaccgcg cgccgtggac
     3961 caatttcaac aatttgcccg agatacatca ccgccacacg atgactaatc cgctctacca
     4021 cggccatatc gtgggagata aacagatacg caatgccgaa atcacgctgg agatcgagca
     4081 acaagttgat aatctgcccg cgaatagaaa catccagcgc cgaaacggct tcgtcggcaa
     4141 tgatcacttt tggattcaat gccaacgcgc gagcaatgca gatgcgctgg cgctgaccgc
     4201 cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa caggcccacg cgctccagca
     4261 accacgcaac gcgtgcagcc gcgtctttac ctggcaataa accgtgtaca cgcagcggtt
     4321 cgataatcga atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg tcctgaaaaa
     4381 taaactgaat atcccggcgc aatgcctgaa gtttgccggg tgacaaggta tcgattctct
     4441 gaccgttaaa gataatttcg ccgccctgcg attcgaccag gcgcagcaac gcccgcccgg
     4501 tagtggattt accgctgcca gactcgccca ccagcgatag cgtttcgcca ggccagagat
     4561 caaaactgac tttctcaacg gcatgcactt cccgcgttac gcgattcaac aaaccgctgc
     4621 gcaaagggaa acgggtgaca agattacgca ctcgtaaaac aggttcgcca tccaccaccg
     4681 ttttctgctc gatggggggg gcctgtttcg ctggatgttc aagcgatatc aacgggaaac
     4741 gtcggggata atccaaccct ttcatcgcac caagttgcgg aacagcagct aacagcgcac
     4801 gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac ggtacccgtt tccaccgcct
     4861 cgccctgata catcaccagt acccgatcgg caatctctgc caccacgccc atatcgtgag
     4921 tgataaagat aacgcccatc gacatctctt tttgcaatac tttgattaat tgcaggatct
     4981 gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc ggcaatcagc accgccgggc
     5041 ggcatgacag cgccatcgca atcatcactc gctggcgcat cccgccagag agttgatgcg
     5101 gataacgtga aagaatggtt tgtgcctcag gaatgcgtac ctgatccagc atccgcttcg
     5161 cctcgaccat cgcttcttca cgactggcgt tctgatgcag acgaattgat tcggcaatct
     5221 gttcacccac agtaaatacc gggttcagcg atgtcatcgg ctcctgaaat atcatcgcca
     5281 tatccgcacc gcgaacatgg cgcatttgtg cagcgctctg ctcgctaagt tcaatcactt
     5341 cgcgactgcg ccgctgcaac agcattttat cgcactgtac taaaccgccc gcctgttcca
     5401 acaggcgcat caatgccaac gcagtcactg acttaccgga gccggattcg ccaacaattg
     5461 ccagcgtctc accgcgttgc agactaaaag agagattgcg gaccgcagct attttctgct
     5521 ggtcctgcat aaaggcaata ttcagatttt caaccgccag cacattaccg gcatcaagtt
     5581 catcactgtg tggcaacggt gtcccctttt tcacggtaga taccggtggt tggcgtatcg
     5641 cctgcgtagc cccaggcgcg atacattcct tcggtgttaa acggtagcgc gacattccct
     5701 tcatggtcga tagcgattaa gccaccgcta ccgccaagcg cagggagttt ttccattact
     5761 acccgctcgc aggcttccgc gagacttaat ccgccgtaat ccattaacgc ggcgatgtca
     5821 tatgccgcca gcgcgcggat gaagacttcg cccgtgccga tacaagaaac cgccacactg
     5881 gcgttattgg cgtagcatcc ggcacccact aagggactat cgccaactcg tccgggtaat
     5941 ttattggtca ttccgcctgt ggacgtggct gccgccaaat tgccgtctaa atccaacgcc
     6001 acggccccca cggtgcccat tttctgtttt tcatccagtg gcgcaccgct atggtcgagg
     6061 acggttgccc cttccttgcg cgctgccagt agttgttcat aacgcaaagg cgtggagaaa
     6121 atctccggcg agacgcgctc catgccacga gcaaacgcaa aattttctgc cccttcgcca
     6181 atcatcatca catgcgggct ttgctccatc accagccggg cggcaagaac cggattacgc
     6241 agatgactaa cgcccgccac cgcaccggct ttcagggtgt taccatccat cacacaggcg
     6301 tccagttcat gggtttcatc acgcgtaaag acagcgccaa ttccggcgtt aaacagtgga
     6361 cactcttcca gcagacgcac cgcttccgtc accacatcca gcgcactttc gcccgcttcc
     6421 agcattttct gcccggtttc aacaatggca gacaacgcct caatgtagcg taattcctgt
     6481 tgcagactca tctgcgcgcg gctaattgca cctgcgccac catgaattgc aatgactgct
     6541 ttgcccatag tattcgtcca ttaagggtgg ctcgccactt ttgctataaa tatcagaatt
     6601 tttatgaatt actcattcaa tttttgaata tagaaagatg gatatggcat gtaaaggcag
     6661 gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac ataataggca
     6721 aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat tgatgtcgct
     6781 cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc aggaaacgct
     6841 gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc cgcttgatgt
     6901 tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg atattgcctc
     6961 cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtatc atggtgaatg
     7021 gcctgcgggc acctgcattc gtattatgac cggtgcgccg gtgccggaag gctgcgaagc
     7081 ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta ctgctgaagt
     7141 gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg cggttgtttt
     7201 cccggcggga actcgcctga ctaccgcaga gctgccagta attgcttcac tggggattgc
     7261 cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg atgaactcca
     7321 gttgcccggt cagccgctgg gcgacggcca aatctacgac accaaccgtc tcgccgtaca
     7381 cctgatgtta gaacagctgg gttgcgaggt aatcaactta gggattatcc gcgacgatcc
     7441 ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtag tgatcagttc
     7501 cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag agctggggga
     7561 gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg gtaaactcag
     7621 caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga ccttctatca
     7681 actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc tgcccgcgcg
     7741 ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg atttccagcg
     7801 cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg gacatcaggg
     7861 ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg aacgcgatcg
     7921 cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt tgttcggagg
     7981 cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca aatcattctg
     8041 cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct gatcgtcggc
     8101 ctcggtggac tcggctgcgc agcttcgctg tatctggcaa gcgccggtgt cggcaacctg
     8161 acgctgctcg acttcgacac ggtttcgctc tcgaatctgc aacgccagac actgcacagt
     8221 gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg acgccctgac gcggatcaac
     8281 ccacatatcg cgattacgcc agtcaatgca ctgctagatg acgcagaact tgcagcattg
     8341 attgctgaac acgatctggt gctcgactgt acggataacg ttgcggtacg taatcaactg
     8401 aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat tcgtatggaa
     8461 gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg ccttagccgt
     8521 ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc gttgatcggc
     8581 gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta tggcaaaccg
     8641 accagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga aatgaaactg
     8701 atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg cctgataaga
     8761 cgcgacaagc gtcgcatcag gcatttgctc cagacgccag atgcggcgtg aacgccttat
     8821 ctggcctacg gttcgggcac agatttccaa gcgcggcagg catcggtgca ctccgccacg
     8881 tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc
     8941 cctgccagtc ctgctcaaac ttcgccacag cggcttccac cgccggatag ctaatcatct
     9001 gttgtgccac atccagtggc agagtaattg attcacatcc tgccagtaag cagtccagcg
     9061 cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc ctgcggcgca tgcattttca
     9121 ataactggtg taagtcggtc acagtctgaa tgccgctacc gccctgagca tcaatacgat
     9181 taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagcaaccct tgtgctgcgc
     9241 catataccgc ggttcccagc gttggaatcc cttccgcttt taacatctta atagctgcca
     9301 gcccctcggc ggtcaccgga actttcacca cgatatccgc aataatagaa cgcaatttac
     9361 gtgcgtcatt caccatccct tcagcagtgg tagccattac ctgggcaaac agacgcccct
     9421 gaccgcccat cgcttcatga agttgcggaa gcacaacatc cagcggtttt ttacccgcgg
     9481 cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac agcgccttca
     9541 ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa atgtactttt
     9601 tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgcaca tcaatctaac
     9661 tttcattcga aagtatttta atgtttgaac gaaacagaga ggcgactatg attttcaata
     9721 ttcagcgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt cttaaaggct
     9781 gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg caggatctgc
     9841 tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc gcgccggaag
     9901 tgattgagcg cgcgctgaat ggtttgctta ttcatcggga aaagttaacc ccggagcatc
     9961 tgacggcgtt aaccgactgc tgtccgacac aggcattaac cgtgtgtggt gaagtgaaaa
    10021 gcgttgagga gatcatgacg accgttctgc gcgataaacc gttttacgat cgcagcggcg
    10081 gcggtttaac gctttcgggt ggtgagccct ttatgcagcc ggaaatggcg atggcgctac
    10141 tgcaagccag ccacgaggca ggcattcata ctgcggtaga aacctgtctg catgtgccgt
    10201 ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat ttaaaacacg
    10261 ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagagtg ctggataacc
    10321 tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg attcagggct
    10381 ttaatgccga cgaaacctct gtaaaagcta ttaccgattt tgccgccgac gagctgcacg
    10441 ttggcgaaat tcatttcctg ccctaccaca cgctaggcat caacaaatat cacttactta
    10501 atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc gactttgccc
    10561 agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa ccatgaccac
    10621 actgaaactg gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat
    10681 tgtgaaaccg ccagtctgta ccgagcgcgc gcagcactat accgagatgt atcaacaaca
    10741 tctcgataag ccgatcccgg tacgtcgcgc gctggcactg gcgcatcacc tggcgaatcg
    10801 caccatctgg atcaaacacg atgagttgat cattggcaac caggcaagcg aagttcgcgc
    10861 cgcgccgatc ttcccggaat atactgtctc gtggatcgaa aaagagattg atgatctggc
    10921 agatcgtccc ggtgctggct ttgcggtgag cgaagagaac aaacgcgttc tgcatgaagt
    10981 gtgcccgtgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt ttaccgatga
    11041 gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa ggcaatatga cctccggcga
    11101 tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg gtctgcgcga
    11161 gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggta ctggaagatt tgcacggcga
    11221 acagttcctg aaagcgattg atatcgtgct ggtggcagtc agcgaacaca ttgaacgttt
    11281 tgcagccctg gcgcgtgaaa tggccgcgac cgaaacccgc gaaagccgtc gcgatgaact
    11341 gctggcgata gcagaaaact gcgatcttat cgcccaccag ccgccgcaga ctttctggca
    11401 ggcgctgcaa ctgtgttact tcatccaatt gatattgcag atcgaatcta acggtcactc
    11461 ggtatcgttt ggtcgtatgg accagtatct ctacccgtac tatcgccgcg acgttgaact
    11521 caaccagaca ctggatcgcg aacacgccat cgaattgctg catagctgct ggctgaaact
    11581 gctggaagtg aacaagatcc gctccggctc acactcaaaa gcctctgcgg gaagtcctct
    11641 gtatcagaac gtcaccattg gcggacaaac tctggttgat ggtcaaccaa tggacgcggt
    11701 gaatccactc tcttacgcta tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa
    11761 cctcagcgtg cgttaccatg caggaatgag taatgatttc ctcgacgcct gcgtacaggt
    11821 gatccgttgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga tcccggaatt
    11881 tattaaactc ggtattgaac cgcaggacgc ttacgactac gcagcgattg gttgtatcga
    11941 aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta tcaacttcgc
    12001 ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc accagcggca aagtgttcct
    12061 gccacaagaa aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag taatggacgc
    12121 gtgggatacg caaatccgtt attacacgcg taaatcaatc gaaatcgaat atgtcgtcga
    12181 caccatgctg gaagagaacg tgcacgatat tctctgctcg gcgctggtgg atgactgtat
    12241 tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat gactgggttt ctggcctgca
    12301 ggttggcatt gccaacctcg gcaacagcct ggcggcagtg aagaaactgg tatttgaaca
    12361 gggcgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg atggcctgac
    12421 tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca acgacgatga
    12481 taccgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac tgaaacagta
    12541 ccataacccg cgctacggtc gtggtccggt tggcggcaac tattacgcgg gtacctcgtc
    12601 tatctccgct aacgtaccgt ttggcgcgca aactatggca acaccggacg ggcgtaaagc
    12661 ccacaccccg ctggcagaag gcgcaagccc ggcctccggt actgaccatc ttggccctac
    12721 tgcggtcatt ggctcagtgg gtaaactgcc tacggcagcg attctcggcg gcgtgttgct
    12781 caaccagaaa ctgaatccgg caacgctgga gaacgaatct gacaagcaga aactgatgat
    12841 cctgctgcgt accttctttg aagtgcataa aggctggcat attcagtaca acatcgtttc
    12901 ccgcgaaacg ctgctggagg cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg
    12961 tgtcgcgggc tattccgcct tcttcaccgc gctctctcca gacgcacagg acgatatcat
    13021 cgcccgtact gaacatatgc tgtaatctcc tcggcccggc ggcgtcgtcg ccgggctaaa
    13081 tcattcacat catcaatttc atccttactt tcattcgaaa tataatttgt gctctgcgtc
    13141 acattgttat tacactgtta cggtttcatt ttcgagccag gagcacacct atgagcgtaa
    13201 aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg tacaaccaac
    13261 cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattaagttc gttgtcgcca
    13321 gcggtaatca gtattaccag cttatttcgt tctttcctga gctaaaggat gagatctctt
    13381 ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcaactgttc cacggcgaac
    13441 tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag caactcaatt
    13501 ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa gcatttgtcg
    13561 cactgatggc aaaacactac catcgcctga aagctgtaaa agattatcag gagattgacg
    13621 acgtactgtt caagttttcg ctcaacctgc cagatgaaca aatcccgtta gtgatcgaca
    13681 aactgcacat agcgctcgat ggcattatga aacccgtcac cagtggtttt ggctttatcg
    13741 acctgattat tcccggtcta cataaagcaa acggtatttc gcggttactg aaacgctggg
    13801 atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg gagatgctga
    13861 aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa caaatcgccc
    13921 gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag gcggtgctgg
    13981 ataacacatc cccttttaac agctgacctc tctaccggag ccgtgcgctc cggttttccc
    14041 ttctttttac atcctgtaca tcacatcaaa tcattaaact tgcattaact tatttataac
    14101 ttatattgtc gcatgcaaat cagaatggtt tcgaatgaaa gttaatgaga ggtggttatg
    14161 gcttctactt ttaccagcga cacattgcct gccgatcaca aagcagctat ccgtcagatg
    14221 aagcacgcgc tgcgggcgca gcttggcgac gtccagcaga tctttaatca gctaagcgat
    14281 gacattgcca cgcgagtggc tgaaatcaac gcactcaaag cacagggcga tgccgtctgg
    14341 ccggtgctgt cttatgccga tatcaaagca ggtcatgtta ctgcagagca gcgcgaacag
    14401 attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca agcgctaggc
    14461 tgggatcagt cgatgctgga ctatctggac cgcaaccgct ttgacgaggt ctacaaaggc
    14521 cccggcgata atttcttcgg gacgctcagc gcttcacgtc ccgagattta ccccatctac
    14581 tggtcgcagg cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc gcagtcgttt
    14641 ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc
    14701 gtcatctacc ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa aggtcttgga
    14761 gcgcataccg actccggggc gctggaacgc tggctgcttc cagcgtatca gcgcgttttc
    14821 gccaacgtct ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc acatcgtacg
    14881 gaagttgaag agtacacggt ggacaacacc accaaatgtt ccgtgtttcg gacattccaa
    14941 ggctggacag cgctctctaa tatgctgcct ggtcaggggc tgctgcacgt tgtgcccatt
    15001 cctgaagcca tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa
    15061 ctgtgcggcg tagcgcccgg aagagtgttg ccggtatcag agcaatggca tccactgtta
    15121 attgaggcgt taaccagcat tccaaaactc gaggccggag actccgtctg gtggcactgc
    15181 gacgtcatcc attccgttgc ccccgttgaa aatcaacagg gttggggcaa cgtgatgtac
    15241 attcctgcgg caccaatgtg cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca
    15301 ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac aaactgggaa
    15361 ggacgcttta cgcttgccga cctcaacatt cacggtaagc gagcgttggg aatcgacagc
    15421 tgagtgtcgt acagcccttc ccgttccacg gcggtgttcc cttttcctgg gcgaacccgc
    15481 cgcactgact cccgcaccgc caatcgtccg tgcagcagca gcgagcccac cggagcatcc
    15541 gggcgaatca gccgctgttg caacatttgc accgcttcat tgcctagctc atcgcgtggc
    15601 acatgcacag ccgtcagggg aacatcctcg attgccgcaa ggttaaagcc gtcgatactc
    15661 atcaccgaga catcctgtgg tacgcgcaga ccacgacgat gtagcgcatt taccgttccc
    15721 gccgccataa aatccccgcc gaccagaaac gccgtcggca acgtcttgcc attggcggca
    15781 tccagccacg ctccgacctt ttcttccgct tctttagcgc taaaactgct gatattcagc
    15841 aaatgacggt catggtgaaa ggccagatta tgctgacgcc atgattcttt aatccctgca
    15901 aaccgctgct ccatagtgta gcggcgcagg cacatcacat tcatcacctc ccggtgaccc
    15961 atttcaaaca gatagcgggc ggcgaacgcg ccaattaacc gatgatccgg cgcaatcgcc
    16021 gggaggcgca ttcgttcatc acggcagttg atcagcacac aaggcttagc gagatcggcg
    16081 gccagatcat ggatgtgcgg atcgtcaatc cccagcagga tcgctgcctg cgtatctgct
    16141 tcattcatcc gcgagagaaa gagcgcggga tcgctgtcaa actcctccag cgcgcagtaa
    16201 cgcagccgca cttcatgcgg cgataacgct ttgctgatgc tctggatcac acggtaataa
    16261 aagatatcag atcgttggtc aaaagcgcgt tgcggcgcga aaataatcag gttgttcagc
    16321 aacatacgac ctgctgccat gccatccatc acgcccaact cacgcgcgca ggccagcacc
    16381 tgttcgcgag cgcgtgcact ggtattcgct ttgccagcca gcacccgcga aaccgtactc
    16441 ggcgatagcc cggtacgtgc ggcgatatcg gcgattttca gcttcttgtc cattttgtga
    16501 tctgcctcta attcggaaat gacagaattt tcatgagcaa aaaagcccat tctgtctggc
    16561 attaaatcca tttcagtaat gtatatcagt cactcatgga aaaacgtgca caaaacccgc
    16621 tttctttcgc ttttagggtc tcttactgtg aattggtaga acaacttcca tacaaaaata
    16681 atggaaaaac gaacaataaa gactttattt atcaaaaagt taaaagtggt gtaacaactc
    16741 cattctgacg ttgccgcaca aaccctatac cattctgtgg agtgcatatg agtcagggta
    16801 tcaataacga tatgactgcc agtaaaccgc gccatatcgt caaaaatctg cgttggtgga
    16861 tactggtcct ttttttgctc ggtgttactg tcaactacat cacgcgtaac tcgctgggta
    16921 ttctcgcccc cgaactgaaa agcagcctag ggattaccac cgaacaatat tcctggattg
    16981 taggtgcttt ccagctcgcc tataccctat tccaaccgtt gtgcggatgg ctgattgacg
    17041 tcattggcct caaactgggc tttatgatct gcgcgggact gtgggcactg gcctgtctga
    17101 tgcatgcggg ggcgggaaac tggggacatc tggcgctgtt gcgcttcttt atgggcggtg
    17161 cggaagccgc ggcaacccca gcaaacgcca aaaccatcgg tgaatggttt ccaaaatcag
    17221 aacgcccgat cgccgcaggc tgggcaggcg tcggtttttc catcggtgcc atgctggccc
    17281 caccgattat ctattttgcc cacgcttcat tcggctggca gggtgcattt atgttcactg
    17341 gcatcctggc gatgctgtgg gtggtgctgt ggtgggcgtt ctatcacagc ccggataagc
    17401 atcctaacct cagcaaagcc gagctggact ttattcgcca ggataacgaa gcgcccccgg
    17461 tcaagttacc ctttctgacc gcactgaaaa ccgtgggcaa aaataaacgc ttttacggta
    17521 tcgctatccc ggcctttatg gcagaaccgg cgtgggcggt gatgagtttc tgggtgcctc
    17581 tatacctgtc caaaacctat ggcatggacc tgaagcagat cgcattattc gcctggttgc
    17641 cgttcctcgc cgctgatctt ggcagtattg ccagcggcta tctgaccaaa ctttacaccc
    17701 gcctgtttgg gctgagtcgt gtgaactccg ttgtcgcatc atcggtcagt ggcgcattct
    17761 tgatggtttc gctggcggtg atggccttta ccaataaccc gtatatcgcc atcgtcctta
    17821 tctccatcgg cggcttcggt caccagatca tctcctgcat gctgagcgca ctggtggtgg
    17881 aatccttcga caaaggtcag atggccactg ttaacggcat gcgcggttct gccgcgtgga
    17941 tcgcaagttt cctgttctcc ctgattatcg gggttaccgc tgacaaaatc ggctttaacc
    18001 ccctgtttat cgccatgggc ttctttgacc tgattggcgc tttcttcctg gtcaccttta
    18061 ttgctgaacg tcgcgcaaaa cgcgcctgag agtggatgta aacatgaaaa cgctgaaaaa
    18121 ctggaccctg cacaaacagt ctgcccacca cgttgaactg ctggtcgatg gccagcatat
    18181 cctgtgcctg tacgtgctgg aagaaaatct gttccgcgtg ctgttaaaac gcaaaggcga
    18241 gctggcgcta gatcgcacct ggagcattgc tccggcgcag gatgtgccat gggaagggcg
    18301 cagccgcgaa gatctcagcg gctttagcct gcccgcgtgg acagtgatcc agcacgacga
    18361 caccgtggtt atcgccaccg agtcactgcg tgcaaccgtt caccagccgc tgtggctgga
    18421 gtggcactat cgtaacaacg aaggcgaatg gctgccgttg gtgaatgacc gcccgaccag
    18481 cgcttatctg ctgaatgccc acggcgatgg cgtggcacac tatcagagcc gtcgtaaaga
    18541 tgaacgcttt tatggcctcg gagataaagc cggcgatctg caacgcaacg gtaaacgtta
    18601 cgagatgcgt aacctcgacg cgatgggcta caacgccgtt agtactgacc cgctgtacaa
    18661 acacattcca ttcaccatca cccgccgcga tgacattagc ttcggcctgt tttatgacaa
    18721 cctcagcagc tgctggctgg atctgggcaa cgagatcgac aactaccaca ccgcctatcg
    18781 ccgctggcag gcggaagcgg gtgatatcga ctactacctg tttaccggcg aacgcgtact
    18841 tgatatcacc aaagccttcg tacgcctgac cggaaaaacc ctgttcggac cgaaatggag
    18901 cctgggctac agcggttcaa ctatgcacta caccgatgcg ccggacgcac aaaatcagtt
    18961 gatgaacttt atccgcctgt gtgaagaaca tgcaattccc tgcgattcgt tccagctctc
    19021 ttccggttat acctcaatta acggcaaacg ctatgtgttc aactggaact acgacaaagt
    19081 gccgcagccg aaagtgatga gccaggcatt tcacaatgct ggtttgcacc tggccgccaa
    19141 tatcaagccg tgcctgttgc aggatcaccc acgctataac gaagtggcag aaagaggact
    19201 gttcatccgt gattcagaaa cagatgaacc agaacgttcc agcttctggg atgacgaagg
    19261 ttctaacctc gagtttacta atccgcagac catccagtgg tggcaggaag gcgtgaccac
    19321 acagcttctg gagatgggta tcgactccac ctggaacgac aacaacgaat tcgaagtatg
    19381 ggacggtgaa gcacgctgtc acggctttgg tcagacgatc gcgatcaaac atattcgccc
    19441 agtgatgcca ctgctgatga tgcgagcctc gatggaagcc cagcagcgtt tcgctccgga
    19501 aaaacgtccg tacttgatct cccgctccgg ctgcgcggga atgcagcgct acgtgcagac
    19561 ctggagtggc gataaccgca ctagctggga cactcttcgc tacaacaccc gcatgggtct
    19621 ggggatgagc ctctccgggc tgtataacct cggtcacgac gtcggcggtt tctccggtga
    19681 taagccggac ccggaactgt ttgtacgctg ggtacaaaac ggcgtgatgc atccgcgctt
    19741 tacgattcac tcatggaacg atgaccatac cgtaaacgaa ccgtggatgt atccgggcgt
    19801 cactccggcg atccgcagcg ctattgagtt gcgctatcgc ctgctgccct acttttatac
    19861 cctgatgtgg caggcatacg ccgatgatga accaatgcta cgtccgactt tccttgacca
    19921 cgagcacgac gttcagactt ttgaagagtg tgatgacttc ctgcttggtc gcgatatttt
    19981 ggttgccagc gtcgtagagc aaggcgagcg ccagcgccgt gtatggctgc cagataacga
    20041 aactggctgg tacgatttct acaacggcga atggttcagt ggcggtcagt ggatcaccct
    20101 cgacgcaccg ctggagaaac tgccgctgat ggttcgtgcc ggggctggtc taccgctcag
    20161 caaacgcatc acctatgtga gcgcagaaca agacgatacc cgcgaactga aactgttccc
    20221 gctgaaaggt gtgggcacaa cgtccggcct gctgtttgaa gacgatggcg aaagctgggg
    20281 ctaccaaact ggtaatgcgc tttgggtgga atgggaaatg gtttgcgatg gggcgaccat
    20341 caacctgaaa gtcaacgcgc gcggggatta tcgtcctgca tggagtgcac tgaaagtgtc
    20401 attaccattg gaagaaaaac gcacgctgct ggtcaatggt gttgaaggga gtgaatggat
    20461 gcgttaaatc attaccgggg ccgggaggcg actgcacctt acccggtcca gccatcgaat
    20521 cctttacctc agtatttcac gctgcaggaa ggcagcaaac ctgtaaaccc cgggagctta
    20581 aataagtaag tgaccggggt gaacaggtga agccaatgca tctgccgcat gaaagacggc
    20641 ggggaattaa gcgtcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc
    20701 gatcgcgcgt tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg
    20761 gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc
    20821 gatggattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg
    20881 catcattaac ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg
    20941 ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc
    21001 gtcatcgcct tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa
    21061 ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt
    21121 gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc
    21181 gttggtaccc agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa
    21241 cagtggaccg ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag
    21301 tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc
    21361 cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt
    21421 ggcgctaaag cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt
    21481 atcggccagc agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc
    21541 cggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag
    21601 gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc
    21661 cagccagcga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatctc
    21721 cggatcagca aacagcgcct gcgccagaag cacacgcagt ttccagccag gagcaacttc
    21781 actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc
    21841 gcgagcttcc gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt
    21901 atagccgtct tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac
    21961 ttcccacaac tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc
    22021 aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa
    22081 agtcggctct aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc
    22141 gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg
    22201 cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga
    22261 gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag
    22321 gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag
    22381 ctggaatgaa cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca
    22441 ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg
    22501 ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga
    22561 gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg
    22621 agtattttgc cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg
    22681 tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc
    22741 cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc
    22801 cgaaagggac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata
    22861 cgcctatcaa ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga
    22921 ccgccaaaat gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg
    22981 caggtccgga taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc
    23041 atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc
    23101 gtaacgaaga catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta
    23161 ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata
    23221 acccgctgtc taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga
    23281 cgaagagcgt gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag
    23341 cgattaaaaa ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa
    23401 aaggcggact cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac
    23461 taacaaaaca tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa
    23521 acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc
    23581 gggtaataac ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac
    23641 atagttcagc aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc
    23701 cgttaaccat aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc
    23761 tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt
    23821 ccagtccaca ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac
    23881 cagtccccac agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag
    23941 acaactccac accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag
    24001 cgcctttcca gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc
    24061 cagcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag
    24121 aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc
    24181 aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg
    24241 aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag
    24301 caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag
    24361 cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt
    24421 gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa
    24481 gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg
    24541 taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc
    24601 gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg
    24661 acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc
    24721 agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc
    24781 atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca
    24841 gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg
    24901 taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc
    24961 tcttccacgt tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc
    25021 attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta
    25081 aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctacat aatatagcct
    25141 gtgctatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg
    25201 aggtcttatg aatgagttca agaagtgtat gcgcgtgttt agtcattctc cctttaaagt
    25261 acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa
    25321 accttccgat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt atgcaggcat
    25381 aacgcgtttt ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag
    25441 atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctacgt
    25501 atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga
    25561 cccttgacgc cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc
    25621 tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt
    25681 tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg
    25741 cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg
    25801 tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc
    25861 ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc
    25921 tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg ctcggcgtaa
    25981 tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc gacagcctgt
    26041 gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg
    26101 gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg
    26161 tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag
    26221 atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggcg
    26281 aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg
    26341 ccagcagcgt taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga
    26401 aatcacttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact
    26461 ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa
    26521 tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc
    26581 tgaaagaagg taatttcgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga
    26641 ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg
    26701 gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga
    26761 aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt
    26821 acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc
    26881 tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc
    26941 aaaatttcca ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta
    27001 aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag
    27061 cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca
    27121 tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc
    27181 cgaaacctta aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat
    27241 tagaagcggt aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc
    27301 tgctcgtaag agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg
    27361 taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat
    27421 ttaccataac ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc
    27481 ggaccagcag tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg
    27541 cttttctcgg tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag
    27601 cggtatttca ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg
    27661 taaccgccag ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact
    27721 gcggccagtg ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata
    27781 agtcctaagt tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct
    27841 aacgggccat ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc
    27901 actttttcag agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat
    27961 tcctaatcac cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa
    28021 aattttttca acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact
    28081 taagtctaga tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg
    28141 ttaccaatag tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct
    28201 aagtcacttt ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga
    28261 acgctgatcc tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg
    28321 ttaccgctgt tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct
    28381 attcagacag taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg
    28441 gcgctgttct cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt
    28501 ctgtggttcc tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg
    28561 ctggcactgg gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga
    28621 gcggaacatg gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg
    28681 ccaattggag cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt
    28741 ctggctgtcg ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc
    28801 cgtttgccaa cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt
    28861 tccgggatga ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct
    28921 atcatcgccg cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa
    28981 ttagacatta attaaattta catttctgca tggttatgca taaccatgca gaatttctcg
    29041 ctacttttcc tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa
    29101 aattcctggc gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc
    29161 tttattagtt ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat
    29221 agcggaacac atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag
    29281 aggatatgaa attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta
    29341 tacccgcaac gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca
    29401 ggttatccag tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg
    29461 cgctaacttc attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca
    29521 tctggatacc atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca
    29581 agttatcaac agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga
    29641 tcacctgaaa gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat
    29701 tggcgaagcg aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga
    29761 taaattcctg tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac
    29821 tttagtaccc gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc
    29881 aacgcactta tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat
    29941 ccacatcatc acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata
    30001 atcactgtgt tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct
    30061 tcagggtatt gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaagga
    30121 gttgcaaaac tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg
    30181 gatagaaaaa aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc
    30241 tggcttttgc ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg
    30301 ccttcgttcc gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt
    30361 gggctgccat cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg
    30421 ggatcattcc ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca
    30481 ccgacgagcg taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag
    30541 tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg
    30601 ttgctgttaa gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag
    30661 atctgcgtca gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag
    30721 acgccgttct gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc
    30781 agttcaaagc ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag
    30841 gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa
    30901 cttacaacga aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct
    30961 gtaaagcgca ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa
    31021 acccgctccg gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac
    31081 aacatatgca gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg
    31141 ccaaaatgac cctgtggatt tcggtcctcg gtctggctgg cggtctggta atcggattgc
    31201 tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta
    31261 ttgaggtgat ccgcggcaca cctatcgtcg tccaggtgat gtttatttat ttcgccctgc
    31321 cgatggcgtt taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga
    31381 tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct attcacaaag
    31441 gttttcgtga agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca
    31501 ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca
    31561 ttaaagacac ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag
    31621 aaattattgc cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc
    31681 tgattattac cctggtgcta agctttattt tgcgtcgtct ggaaagaagg atgaaaatcc
    31741 tgtgattgaa tttaaaaacg tctccaagca ctttggccca acccaggtgc ttcacaatat
    31801 cgatttgaac attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa
    31861 atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt
    31921 cgatggcctg aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg
    31981 tatggtgttc cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt
    32041 tggtccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct
    32101 gctggcgaaa gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg
    32161 tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt
    32221 tgatgaaccg acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca
    32281 ggatctggct gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga
    32341 gaaagtagct tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc
    32401 gcaggtattg atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc
    32461 ttaacttctc tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa
    32521 atcgcccctc ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc
    32581 atgcggtgga tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc
    32641 atacccggcg ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg
    32701 gatatcgaac aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc
    32761 tcgcgtaaag aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg
    32821 gtcccgaaga tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc
    32881 gaagtcagcc gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat
    32941 atcaccggct ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat
    33001 ttttcaatgt tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg
    33061 ccgctgtatc gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg
    33121 ttgcagcttc cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg
    33181 acattatttg tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct
    33241 ttccaacaaa gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta
    33301 cgcctgattt tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca ggacgagagc
    33361 gcccgttact ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt
    33421 gtggccgtgc cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc
    33481 atcattatgc tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag
    33541 attacccagc atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc
    33601 cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc
    33661 ttttcgttgt tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc
    33721 ctggcgatta ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcaaaa
    33781 accatcactc tctcgccaca tactcagcgt aactatccag agttgcaaaa acggctgaat
    33841 ggctggcttt cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg
    33901 ttgctgttga gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg
    33961 cagaaaaccg tagatatcct gatccgtatc gcacttattc tttttttctc ggcggttggc
    34021 tggacggtgc tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg
    34081 ctacccagcg cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt
    34141 atcagtacca tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg
    34201 ctggcaggtg ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa
    34261 gatatcatta ccggggtgtt tattcagttt gaaaacggca tgaacactgg agatttggtg
    34321 actatcgggc cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc
    34381 caggataccg gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc
    34441 gtccgcggta ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat
    34501 aaagccaatc aggcactgaa agatgcggta gcggaattaa tggaaaacga agaaattcgc
    34561 gggctgatta ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa taccgcgttt
    34621 acactgcgtg tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc
    34681 gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat
    34741 caggtgctgc ctactccggg cgcgaccccg gctgaaccgt taccgccggg ggagccaacg
    34801 ctttaacgct ggcgattgac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca
    34861 ataaagcgac tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc
    34921 acgtcggtca gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta
    34981 aaccacatca cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg
    35041 acttcaccac cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct
    35101 ttgttcagcc ccaggttacg gcgtttacgc tcactacctg cccgcgctgc agcggcggaa
    35161 tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg
    35221 ttaaaaattg ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga
    35281 ttagcactga taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta
    35341 aaacgccagc catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg
    35401 atatccagaa tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca
    35461 aggtgatgaa tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata
    35521 tcccagttcg ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc
    35581 agcggattgg caaagtctac gctttgctcc ccggcgggtg taagcgtgag gaattgcctg
    35641 agttcaggat tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc
    35701 ggatgcaacc ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata
    35761 cccgcataac gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca
    35821 tggctatggt ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttaatttgtc
    35881 ttattttgta aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca
    35941 tttgcatcgc gcaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt
    36001 cattatgaaa aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac
    36061 ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac
    36121 cgtttcggca actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc
    36181 gcgcgaacaa ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt
    36241 cggtactgca accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca
    36301 gttttcgcct gttttgtaat caaaaaacat caaatatgaa ctcaatgtaa ataaatgtat
    36361 ttctttttcg cgcaatgggt gatagaaaat cgcgccaagt gataatgctt atcaaaatta
    36421 ttatcacttg catgaacact atcacgggat taacagtggc atcgcatccg cagagatgct
    36481 ttctcgtggc agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa
    36541 tcgcaatttc cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg
    36601 catcacgcct gtggctcagg cactcgccgc cgaagggcaa actaacgcgg atgacacgct
    36661 ggttgtcgaa gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt
    36721 ctcgcgtccg gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa
    36781 agatcagggc gcaaccaacc ttaccgacgc gctcaaaaac gcccccggcg
//
LOCUS       DAZOXR010000018        36331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZOXR010000018 DAZOXR010000000
VERSION     DAZOXR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.018"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="ACHUNN_000787"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="ACHUNN_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR0575247.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /gene="yqfG"
                     /locus_tag="ACHUNN_000788"
     CDS             1867..1992
                     /gene="yqfG"
                     /locus_tag="ACHUNN_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0575248.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="ACHUNN_000789"
     CDS             complement(1989..2060)
                     /locus_tag="ACHUNN_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575249.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="ACHUNN_000790"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="ACHUNN_000790"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0575250.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="ACHUNN_000791"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="ACHUNN_000791"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0575251.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="ACHUNN_000792"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="ACHUNN_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0575252.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="ACHUNN_000793"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="ACHUNN_000793"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0575253.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="ACHUNN_000794"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="ACHUNN_000794"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0575254.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="ACHUNN_000795"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="ACHUNN_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0575255.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="ACHUNN_000796"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="ACHUNN_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0575256.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="ACHUNN_000797"
     CDS             complement(9465..9872)
                     /locus_tag="ACHUNN_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0575257.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="ACHUNN_000798"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="ACHUNN_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0575258.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="ACHUNN_000799"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="ACHUNN_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0575259.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="ACHUNN_000800"
     CDS             complement(11538..12197)
                     /locus_tag="ACHUNN_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0575260.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /gene="yqfB"
                     /locus_tag="ACHUNN_000801"
     CDS             complement(12361..12672)
                     /gene="yqfB"
                     /locus_tag="ACHUNN_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR0575261.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="ACHUNN_000802"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="ACHUNN_000802"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0575262.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="ACHUNN_000803"
     CDS             complement(14207..14950)
                     /locus_tag="ACHUNN_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0575263.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="ACHUNN_000804"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="ACHUNN_000804"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0575264.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="ACHUNN_000805"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="ACHUNN_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0575265.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="ACHUNN_000806"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="ACHUNN_000806"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0575266.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="ACHUNN_000807"
     CDS             20005..20088
                     /locus_tag="ACHUNN_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575267.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="ACHUNN_000808"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="ACHUNN_000808"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0575268.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="ACHUNN_000809"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="ACHUNN_000809"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0575269.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="ACHUNN_000810"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="ACHUNN_000810"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0575270.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="ACHUNN_000811"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="ACHUNN_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0575271.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="ACHUNN_000812"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="ACHUNN_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0575272.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="ACHUNN_000813"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUNN_000813"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="ACHUNN_000814"
     CDS             25234..25836
                     /locus_tag="ACHUNN_000814"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0575273.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="ACHUNN_000815"
     CDS             complement(25937..25996)
                     /locus_tag="ACHUNN_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0575274.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="ACHUNN_000816"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="ACHUNN_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0575275.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="ACHUNN_000817"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="ACHUNN_000817"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0575276.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="ACHUNN_000818"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="ACHUNN_000818"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0575277.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="ACHUNN_000819"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="ACHUNN_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0575278.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="ACHUNN_000820"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="ACHUNN_000820"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0575279.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="ACHUNN_000821"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="ACHUNN_000821"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0575280.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="ACHUNN_000822"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="ACHUNN_000822"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0575281.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="ACHUNN_000823"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="ACHUNN_000823"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR0575282.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36331)
                     /locus_tag="ACHUNN_000824"
     CDS             complement(35716..>36331)
                     /locus_tag="ACHUNN_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0575283.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8703 a         9742 c         9170 g         8716 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatcacaat t
//
LOCUS       DAZOXR010000019        36142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZOXR010000019 DAZOXR010000000
VERSION     DAZOXR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.019"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUNN_000825"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUNN_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0575284.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUNN_000826"
     CDS             complement(280..873)
                     /locus_tag="ACHUNN_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0575285.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUNN_000827"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUNN_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0575286.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUNN_000828"
     CDS             1788..2183
                     /locus_tag="ACHUNN_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0575287.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUNN_000829"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUNN_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0575288.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUNN_000830"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUNN_000830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0575289.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUNN_000831"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUNN_000831"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0575290.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUNN_000832"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUNN_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0575291.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUNN_000833"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUNN_000833"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0575292.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUNN_000834"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUNN_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0575293.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUNN_000835"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUNN_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0575294.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUNN_000836"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUNN_000836"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0575295.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUNN_000837"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUNN_000837"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0575296.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUNN_000838"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUNN_000838"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0575297.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUNN_000839"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUNN_000839"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0575298.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUNN_000840"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUNN_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR0575299.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUNN_000841"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUNN_000841"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0575300.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUNN_000842"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUNN_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0575301.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUNN_000843"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUNN_000843"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0575302.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUNN_000844"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUNN_000844"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0575303.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUNN_000845"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUNN_000845"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR0575304.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUNN_000846"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUNN_000846"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0575305.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUNN_000847"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUNN_000847"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0575306.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUNN_000848"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUNN_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR0575307.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUNN_000849"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUNN_000849"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0575308.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUNN_000850"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUNN_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0575309.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUNN_000851"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUNN_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0575310.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUNN_000852"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUNN_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0575311.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUNN_000853"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUNN_000853"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0575312.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUNN_000854"
     CDS             33227..34570
                     /locus_tag="ACHUNN_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0575313.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHUNN_000855"
     CDS             complement(34650..34856)
                     /locus_tag="ACHUNN_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0575314.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHUNN_000856"
     CDS             35181..35735
                     /locus_tag="ACHUNN_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0575315.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..>36142)
                     /gene="ytfI"
                     /locus_tag="ACHUNN_000857"
     CDS             complement(35825..>36142)
                     /gene="ytfI"
                     /locus_tag="ACHUNN_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR0575316.1"
                     /translation="WYIHEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDED
                     YSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICKKYKSTIEAFKKEQLA
                     LPV"
BASE COUNT         8724 a         9724 c         9045 g         8649 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac ca
//
LOCUS       DAZOXR010000020        34993 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZOXR010000020 DAZOXR010000000
VERSION     DAZOXR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.020"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUNN_000858"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUNN_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0575317.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUNN_000859"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUNN_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0575318.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUNN_000860"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUNN_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0575319.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUNN_000861"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUNN_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0575320.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUNN_000862"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUNN_000862"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0575321.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUNN_000863"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUNN_000863"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0575322.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUNN_000864"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUNN_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0575323.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUNN_000865"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUNN_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0575324.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHUNN_000866"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHUNN_000866"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0575325.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHUNN_000867"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHUNN_000867"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0575326.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHUNN_000868"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHUNN_000868"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0575327.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHUNN_000869"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHUNN_000869"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0575328.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUNN_000870"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUNN_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0575329.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUNN_000871"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUNN_000871"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0575330.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUNN_000872"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUNN_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0575331.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUNN_000873"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUNN_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0575332.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHUNN_000874"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHUNN_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0575333.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUNN_000875"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUNN_000875"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0575334.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUNN_000876"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUNN_000876"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0575335.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUNN_000877"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUNN_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0575336.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUNN_000878"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUNN_000878"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0575337.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUNN_000879"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUNN_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0575338.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUNN_000880"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUNN_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0575339.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUNN_000881"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUNN_000881"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0575340.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHUNN_000882"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHUNN_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUNN_000883"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUNN_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0575341.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHUNN_000884"
     CDS             complement(31717..33003)
                     /locus_tag="ACHUNN_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0575342.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHUNN_000885"
     CDS             complement(33054..33995)
                     /locus_tag="ACHUNN_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0575343.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHUNN_000886"
     CDS             complement(34010..34780)
                     /locus_tag="ACHUNN_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0575344.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         8355 a         9716 c         9169 g         7753 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcctg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca cca
//
LOCUS       DAZOXR010000021        34981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZOXR010000021 DAZOXR010000000
VERSION     DAZOXR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.021"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUNN_000887"
     CDS             complement(<1..815)
                     /locus_tag="ACHUNN_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0575345.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUNN_000888"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUNN_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUNN_000889"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUNN_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHUNN_000890"
     CDS             complement(1397..1873)
                     /locus_tag="ACHUNN_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0575346.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUNN_000891"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUNN_000891"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0575347.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUNN_000892"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUNN_000892"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0575348.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUNN_000893"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUNN_000893"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0575349.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUNN_000894"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUNN_000894"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0575350.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUNN_000895"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUNN_000895"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0575351.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHUNN_000896"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="ACHUNN_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0575352.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHUNN_000897"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="ACHUNN_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0575353.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="ACHUNN_000898"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="ACHUNN_000898"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0575354.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="ACHUNN_000899"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="ACHUNN_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0575355.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="ACHUNN_000900"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="ACHUNN_000900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0575356.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="ACHUNN_000901"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="ACHUNN_000901"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0575357.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="ACHUNN_000902"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="ACHUNN_000902"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0575358.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="ACHUNN_000903"
     CDS             13848..14552
                     /locus_tag="ACHUNN_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0575359.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14823..15470
                     /locus_tag="ACHUNN_000904"
     CDS             14823..15470
                     /locus_tag="ACHUNN_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0575360.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(15535..16246)
                     /locus_tag="ACHUNN_000905"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="ACHUNN_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHUNN_000906"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="ACHUNN_000906"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0575361.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="ACHUNN_000907"
     CDS             complement(17484..18245)
                     /locus_tag="ACHUNN_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0575362.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="ACHUNN_000908"
     CDS             18378..18788
                     /locus_tag="ACHUNN_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0575363.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="ACHUNN_000909"
     CDS             complement(18750..19856)
                     /locus_tag="ACHUNN_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0575364.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="ACHUNN_000910"
     CDS             complement(19867..21000)
                     /locus_tag="ACHUNN_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0575365.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHUNN_000911"
     CDS             complement(20993..22729)
                     /locus_tag="ACHUNN_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0575366.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHUNN_000912"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHUNN_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0575367.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHUNN_000913"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHUNN_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0575368.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHUNN_000914"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHUNN_000914"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0575369.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHUNN_000915"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHUNN_000915"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0575370.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHUNN_000916"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHUNN_000916"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0575371.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHUNN_000917"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHUNN_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0575372.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHUNN_000918"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHUNN_000918"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0575373.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHUNN_000919"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHUNN_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0575374.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHUNN_000920"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHUNN_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0575375.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHUNN_000921"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHUNN_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0575376.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHUNN_000922"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHUNN_000922"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0575377.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="ACHUNN_000923"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="ACHUNN_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8273 a         8976 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAZOXR010000022        34787 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZOXR010000022 DAZOXR010000000
VERSION     DAZOXR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.022"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            450..602
                     /gene="mokC"
                     /locus_tag="ACHUNN_000924"
     CDS             450..602
                     /gene="mokC"
                     /locus_tag="ACHUNN_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0575378.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="ACHUNN_000925"
     CDS             complement(706..1836)
                     /gene="dnaJ"
                     /locus_tag="ACHUNN_000925"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0575379.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="ACHUNN_000926"
     CDS             complement(1925..3841)
                     /gene="dnaK"
                     /locus_tag="ACHUNN_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0575380.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4218..4622
                     /locus_tag="ACHUNN_000927"
     CDS             4218..4622
                     /locus_tag="ACHUNN_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0575381.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4648..5361
                     /gene="msyB"
                     /locus_tag="ACHUNN_000928"
     CDS             4648..5361
                     /gene="msyB"
                     /locus_tag="ACHUNN_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0575382.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5510..6076
                     /gene="satP"
                     /locus_tag="ACHUNN_000929"
     CDS             5510..6076
                     /gene="satP"
                     /locus_tag="ACHUNN_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0575383.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6111..6698)
                     /gene="mog"
                     /locus_tag="ACHUNN_000930"
     CDS             complement(6111..6698)
                     /gene="mog"
                     /locus_tag="ACHUNN_000930"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0575384.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6813..7766)
                     /gene="tal"
                     /locus_tag="ACHUNN_000931"
     CDS             complement(6813..7766)
                     /gene="tal"
                     /locus_tag="ACHUNN_000931"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0575385.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8045..9474
                     /locus_tag="ACHUNN_000932"
                     /pseudo
     CDS             8045..9474
                     /locus_tag="ACHUNN_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9544..10320
                     /gene="yaaA"
                     /locus_tag="ACHUNN_000933"
     CDS             9544..10320
                     /gene="yaaA"
                     /locus_tag="ACHUNN_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0575386.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10473..10769)
                     /locus_tag="ACHUNN_000934"
     CDS             complement(10473..10769)
                     /locus_tag="ACHUNN_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0575387.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="ACHUNN_000935"
     CDS             complement(10983..12269)
                     /gene="thrC"
                     /locus_tag="ACHUNN_000935"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0575388.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="ACHUNN_000936"
     CDS             complement(12270..13202)
                     /gene="thrB"
                     /locus_tag="ACHUNN_000936"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0575389.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="ACHUNN_000937"
     CDS             complement(13204..15666)
                     /gene="thrA"
                     /locus_tag="ACHUNN_000937"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0575390.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15693..15812)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="ACHUNN_000938"
     CDS             complement(15748..15813)
                     /gene="thrL"
                     /locus_tag="ACHUNN_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0575391.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16027..16713)
                     /locus_tag="ACHUNN_000939"
     CDS             complement(16027..16713)
                     /locus_tag="ACHUNN_000939"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0575392.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17113..17253)
                     /gene="yjjY"
                     /locus_tag="ACHUNN_000940"
     CDS             complement(17113..17253)
                     /gene="yjjY"
                     /locus_tag="ACHUNN_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0575393.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17349..18065
                     /gene="arcA"
                     /locus_tag="ACHUNN_000941"
     CDS             17349..18065
                     /gene="arcA"
                     /locus_tag="ACHUNN_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0575394.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18125..19477)
                     /gene="creD"
                     /locus_tag="ACHUNN_000942"
     CDS             complement(18125..19477)
                     /gene="creD"
                     /locus_tag="ACHUNN_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0575395.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19535..20959)
                     /gene="creC"
                     /locus_tag="ACHUNN_000943"
     CDS             complement(19535..20959)
                     /gene="creC"
                     /locus_tag="ACHUNN_000943"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0575396.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20959..21648)
                     /gene="creB"
                     /locus_tag="ACHUNN_000944"
     CDS             complement(20959..21648)
                     /gene="creB"
                     /locus_tag="ACHUNN_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0575397.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21661..22134)
                     /gene="creA"
                     /locus_tag="ACHUNN_000945"
     CDS             complement(21661..22134)
                     /gene="creA"
                     /locus_tag="ACHUNN_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0575398.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22345..23214
                     /gene="robA"
                     /locus_tag="ACHUNN_000946"
     CDS             22345..23214
                     /gene="robA"
                     /locus_tag="ACHUNN_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0575399.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="ACHUNN_000947"
     CDS             complement(23211..23858)
                     /gene="gpmB"
                     /locus_tag="ACHUNN_000947"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0575400.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23910..24431
                     /gene="yjjX"
                     /locus_tag="ACHUNN_000948"
     CDS             23910..24431
                     /gene="yjjX"
                     /locus_tag="ACHUNN_000948"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR0575401.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="ACHUNN_000949"
     CDS             complement(24516..24842)
                     /gene="trpR"
                     /locus_tag="ACHUNN_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0575402.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="ACHUNN_000950"
     CDS             complement(24932..26869)
                     /gene="sltY"
                     /locus_tag="ACHUNN_000950"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0575403.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27080..28747
                     /gene="ettA"
                     /locus_tag="ACHUNN_000951"
     CDS             27080..28747
                     /gene="ettA"
                     /locus_tag="ACHUNN_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0575404.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28908..29015)
                     /gene="nadS"
                     /locus_tag="ACHUNN_000952"
                     /pseudo
     CDS             complement(28908..29015)
                     /gene="nadS"
                     /locus_tag="ACHUNN_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="ACHUNN_000953"
     CDS             complement(29054..30286)
                     /gene="nadR"
                     /locus_tag="ACHUNN_000953"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0575405.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30307..31689)
                     /gene="radA"
                     /locus_tag="ACHUNN_000954"
     CDS             complement(30307..31689)
                     /gene="radA"
                     /locus_tag="ACHUNN_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0575406.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31738..32706)
                     /gene="serB"
                     /locus_tag="ACHUNN_000955"
     CDS             complement(31738..32706)
                     /gene="serB"
                     /locus_tag="ACHUNN_000955"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0575407.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32812..33456
                     /locus_tag="ACHUNN_000956"
     CDS             32812..33456
                     /locus_tag="ACHUNN_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0575408.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33484..34500
                     /gene="lplA"
                     /locus_tag="ACHUNN_000957"
     CDS             33484..34500
                     /gene="lplA"
                     /locus_tag="ACHUNN_000957"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR0575409.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34532..34787
                     /locus_tag="ACHUNN_000958"
                     /pseudo
     CDS             34532..34787
                     /locus_tag="ACHUNN_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8248 a         9427 c         8656 g         8456 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa taggcgaggc
      241 gtcttcagga ttagaatcga taaaacagaa tgggttaaag agagcgatat aatagcggcg
      301 ggtgcttgag gctatctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
      361 tgatatttta ctatcaaccc gaggccccct atatgctgaa cacatgtaga gtgcctctta
      421 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
      481 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa gacctctgtg
      541 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
      601 aagagcaacg gcggggagtg atccccgcca cttttcaggt gctcgcatat cttcaacgca
      661 ccctaatttt acccaagcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
      721 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcacc
      781 ggttgggcca ccgaagcttt cttgcagctc ttgcagcaac tgtttctgct tctcgttcag
      841 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc caccgcggac
      901 agacttgacg cctttaccgc gcatacggaa aagcttaccg gtctgggttt cgccaggcac
      961 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     1021 agcgaagttg atcgggactt cgcaatagag gttgttgcct tcacgctcga aaatcgggtg
     1081 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     1141 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     1201 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     1261 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaaa atccctggcg
     1321 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     1381 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tctctttggt
     1441 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     1501 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     1561 atcgctgaag tctgcgccgc cgccaaaacc gccgccgccc atgccacctt gctcaaacgc
     1621 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     1681 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     1741 gtatttcatg gccaggcgtt tgtaggcctt tttgatttca cgctcttccg ctgttttgga
     1801 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga
     1861 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttatagg
     1921 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     1981 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     2041 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     2101 tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata gcagttttgt
     2161 cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg tgcagcagat
     2221 ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag
     2281 cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa gaagccttga
     2341 tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aagataccgt
     2401 cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac
     2461 catccaggtt gaactgaccc agagatttgt tatcagctgc acgtttacgt tcaccctgca
     2521 gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct
     2581 tggtcgggat agtggtattt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga
     2641 tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca ccagtcagaa
     2701 caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg
     2761 gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac
     2821 ccacgaggat aacgtcgtcg atatcagata cggacaggcc agcgtcctgc agtgcaactt
     2881 tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag
     2941 tcactttgat gttcatgtgt ttcggaccgg tcgcatctgc agtgatgtac ggcaggttaa
     3001 cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc
     3061 gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa
     3121 ccaggtagtt gatcagacgg ctgtcgaagt cttcaccccc caggtgggta tcaccgttgg
     3181 tagccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat
     3241 cgaaagtacc accacccagg tcataaaccg cgatagtacg gttgccagta cctttgtcca
     3301 gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag
     3361 cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg
     3421 tgataacagc ttcagttacc ggttcaccca agtaatcttc agcggttttc ttcatttttt
     3481 tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg
     3541 cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt
     3601 cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt
     3661 tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg
     3721 caataataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggagtggtgc
     3781 catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca
     3841 tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga
     3901 aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact
     3961 actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt
     4021 gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata
     4081 taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac
     4141 gtacctgaat cttctcttta tcagcagtaa actagtgggt attcatccct ctacctcttc
     4201 ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc
     4261 tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc
     4321 cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca
     4381 tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag
     4441 ccgcccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca
     4501 ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct
     4561 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     4621 aacgccagaa tatagaaacc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     4681 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
     4741 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     4801 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag
     4861 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
     4921 attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt
     4981 gagattgagc aacagttact ggaacaattt ctgcgtaaca cctggaagaa tatggacgag
     5041 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     5101 ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc
     5161 caattgaccc gcattttacg cacccacgca gcaatgagcg tgcttgggca tggtttgctg
     5221 cgcggcgcgg gtctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     5281 ggcagtgcct atcgcgtgac aattccagcc gtaatacaaa tcgcctgcct gcgccggatg
     5341 gttagcgcca ctcaggtctg acaccgtaaa ccggggaaaa ttttcatttt cccctcaaaa
     5401 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actactgtta
     5461 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     5521 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     5581 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     5641 ctacggcggc atcgcgcaaa tttttgccgg tctgctggag tacaaaaaag gcaacacttt
     5701 cggtttaacc gcattcacct cttacggttc tttctggttg acgctggttg cgattctgct
     5761 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     5821 tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     5881 gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg gtaacattgc
     5941 cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg gtgccagtgc
     6001 aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat
     6061 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     6121 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     6181 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca
     6241 acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     6301 ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca
     6361 gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg
     6421 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     6481 gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg
     6541 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     6601 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     6661 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     6721 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     6781 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     6841 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     6901 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     6961 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc
     7021 agcagtgccg gtgcgatggt aagacggtcg cagcctgcca gttccagaat ttcgccgatg
     7081 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     7141 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcagta
     7201 ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa cacgcctgct
     7261 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     7321 tctttttcca gctgttctgc agcacggata ccctgccaag tagaagccag tttgatcaga
     7381 atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca
     7441 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     7501 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     7561 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     7621 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     7681 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     7741 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     7801 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     7861 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     7921 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     7981 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     8041 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     8101 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     8161 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     8221 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     8281 cgttgcgctg gctattaccg ccggtggacc tggagccgtc ttttggatgt gggttgccgc
     8341 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
     8401 tgacgtcaat ggacagtttc gtggaggacc ggcatggtat atggcgcgcg ggctggggat
     8461 gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     8521 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt cttttgattt tcccccgctg
     8581 gtgacaggca ttattctcgc tgtctttgct ctgctggcaa tcactcgcgg tcttcatggc
     8641 gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga taatctgggt gctgaccagc
     8701 atggtgattt gcgtaataaa tatcgggcaa cttccccacg tcatttggtc tatttttgag
     8761 agtgcttttg gctggcagga agcggcaggc ggcgcggcgg gatatacctt aagccaggcg
     8821 attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg ttcgacgcca
     8881 aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg gattgtccag
     8941 atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat gctgatatta
     9001 ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat ccagaaggcg
     9061 atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt tattctgttt
     9121 gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt ctttttacgc
     9181 ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac ggtcatcggc
     9241 ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat ggcctgcatg
     9301 gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac cattgccagt
     9361 gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt gcgttatccg
     9421 gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga gtaatcacaa
     9481 ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct gcaaggactg
     9541 gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc gttgaccacc
     9601 acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca tgaagcgcgg
     9661 aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact ggcgggtatc
     9721 aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc ccgccaggct
     9781 attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt cagcgaagac
     9841 gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg cgtactgcgc
     9901 ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga gaatgcccga
     9961 gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa cgaggcgctc
    10021 gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt taaatcggtg
    10081 aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga gaagaacggc
    10141 aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag tcgtttcatt
    10201 attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga aggttacttc
    10261 tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga gcagcgttaa
    10321 gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcatgcaga
    10381 ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg catcaggcgc
    10441 tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc tggaccatga
    10501 ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg cggtccgtgt
    10561 gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag
    10621 tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac
    10681 gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac cagggaaagt
    10741 gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg
    10801 gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca atcctaatct
    10861 ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc tccataattt
    10921 cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga
    10981 ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg cagattatgt
    11041 gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg
    11101 agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag gaacaagcca
    11161 tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc gtgcggctcc
    11221 gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg
    11281 gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc
    11341 ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg agtcgctttg
    11401 ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt ggtcgcagca
    11461 ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt caaatcgccg
    11521 aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg cagctgcgca
    11581 actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat cgagttagcc
    11641 gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt caccagcgcc
    11701 tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caacgtacag
    11761 aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca
    11821 ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga ggtcgcggtc
    11881 agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg
    11941 ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt
    12001 tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc ttccaggatt
    12061 tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa
    12121 tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc atgcggaaaa
    12181 aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct gacctgctcg
    12241 ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta
    12301 tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag
    12361 tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt cgggccggag
    12421 ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc ctgccggaag
    12481 ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca
    12541 agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg cccgtgcgca
    12601 atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact
    12661 ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac ttgctggctg
    12721 atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg tgccacgttg
    12781 tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc cagcaaacgg
    12841 gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccaccgaa
    12901 caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt catcgccact
    12961 ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac gatattttcc
    13021 cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct
    13081 gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt caccgccgcc
    13141 ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc
    13201 atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga caccagcagc
    13261 ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct gataatagtg
    13321 gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat cattcccatc
    13381 cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa catagcgcaa
    13441 aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt cgagctgtga
    13501 cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg
    13561 ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga gaatcaatag
    13621 tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat
    13681 ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat
    13741 ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac ctgcattgag
    13801 cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa
    13861 tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca tcgacgaggt
    13921 gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt cggcatattg
    13981 atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca gatgatattc
    14041 tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg caagttcttc
    14101 ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg agttggcaac
    14161 gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac gcttcagttg
    14221 ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc
    14281 ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttatttac
    14341 cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt tgatattggc
    14401 gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca taccatcacc
    14461 taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta agccttcttt
    14521 cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt cgctttgcgg
    14581 aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg
    14641 ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt tcatccccgg
    14701 gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc
    14761 gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc cggtattttt
    14821 aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt
    14881 agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca acaacctcgc
    14941 atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc aaatctcgca
    15001 acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac cgttgcgtcc
    15061 aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca gcaccatgtg
    15121 atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat cgacggtaga
    15181 ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg taacgttgtg
    15241 accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat
    15301 cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat gcaggacatg
    15361 ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg ggaatcccgg
    15421 ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat cgctgatatt
    15481 gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt ggttggtgat
    15541 tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc tttccagaat
    15601 atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac
    15661 tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg
    15721 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
    15781 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
    15841 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
    15901 taatttactc atggcaggta accagttcag aagctgctat cagacactct ttttttaatc
    15961 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
    16021 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
    16081 gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt ttatgtcatc
    16141 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
    16201 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
    16261 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
    16321 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
    16381 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
    16441 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
    16501 gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa taacatcacc
    16561 agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat cgacaatccg
    16621 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
    16681 ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga ccaaataacc
    16741 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
    16801 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
    16861 aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
    16921 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
    16981 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
    17041 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
    17101 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
    17161 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
    17221 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
    17281 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
    17341 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
    17401 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
    17461 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
    17521 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
    17581 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
    17641 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
    17701 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
    17761 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
    17821 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
    17881 aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
    17941 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
    18001 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
    18061 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
    18121 aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg gctttcattt
    18181 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
    18241 ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg agcagtcccc
    18301 acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg cgccagcctt
    18361 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
    18421 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
    18481 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
    18541 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
    18601 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg aggaagtttt
    18661 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
    18721 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
    18781 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
    18841 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
    18901 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
    18961 cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc accacaccaa
    19021 taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg atatttggcg
    19081 cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
    19141 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
    19201 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
    19261 cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca acaagttttt
    19321 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
    19381 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
    19441 gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt gcaatggcag
    19501 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
    19561 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
    19621 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
    19681 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
    19741 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
    19801 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
    19861 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
    19921 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
    19981 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
    20041 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
    20101 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
    20161 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
    20221 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
    20281 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
    20341 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
    20401 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
    20461 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    20521 ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac tgaccacgca
    20581 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    20641 cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg cgcactttgt
    20701 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgagcca
    20761 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    20821 ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    20881 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    20941 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    21001 aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
    21061 atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca ttagttgctg
    21121 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    21181 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    21241 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    21301 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    21361 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    21421 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga tatcaggcaa
    21481 accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
    21541 gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    21601 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
    21661 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    21721 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    21781 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    21841 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc
    21901 cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg
    21961 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    22021 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    22081 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    22141 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    22201 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    22261 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    22321 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    22381 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    22441 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    22501 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    22561 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    22621 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    22681 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    22741 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    22801 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    22861 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    22921 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
    22981 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    23041 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac
    23101 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
    23161 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
    23221 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
    23281 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    23341 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    23401 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    23461 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    23521 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    23581 atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    23641 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
    23701 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    23761 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    23821 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    23881 taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg accaccaatc
    23941 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    24001 atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa
    24061 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    24121 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    24181 aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc
    24241 tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
    24301 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    24361 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
    24421 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    24481 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    24541 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    24601 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
    24661 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    24721 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    24781 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    24841 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    24901 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    24961 tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa gccagcacgt
    25021 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    25081 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    25141 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    25201 caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    25261 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    25321 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    25381 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
    25441 ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga acgctaagat
    25501 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
    25561 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    25621 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    25681 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
    25741 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    25801 gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    25861 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
    25921 gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat ttcgcctgct
    25981 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    26041 gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt gcattttcag
    26101 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
    26161 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    26221 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    26281 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
    26341 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    26401 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    26461 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    26521 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    26581 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    26641 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    26701 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    26761 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    26821 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    26881 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    26941 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    27001 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    27061 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    27121 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    27181 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    27241 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttacct
    27301 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    27361 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    27421 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    27481 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    27541 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg
    27601 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    27661 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca ccgtggtggc
    27721 gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aacttgaccg
    27781 cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga aagatcagcg
    27841 cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    27901 gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt
    27961 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    28021 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    28081 tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat
    28141 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg gtcaggaaca
    28201 gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg ttgatcagtt
    28261 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    28321 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    28381 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    28441 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    28501 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    28561 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    28621 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    28681 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    28741 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    28801 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    28861 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    28921 tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt aatctcgaca
    28981 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    29041 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    29101 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    29161 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    29221 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    29281 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    29341 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    29401 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    29461 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    29521 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    29581 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    29641 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    29701 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    29761 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    29821 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    29881 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    29941 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    30001 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    30061 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    30121 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    30181 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    30241 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    30301 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    30361 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    30421 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    30481 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    30541 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    30601 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    30661 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    30721 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    30781 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    30841 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    30901 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    30961 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    31021 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    31081 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    31141 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    31201 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    31261 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    31321 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    31381 gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa
    31441 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    31501 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    31561 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    31621 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    31681 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    31741 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    31801 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    31861 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    31921 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    31981 gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    32041 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    32101 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    32161 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    32221 cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    32281 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    32341 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    32401 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    32461 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    32521 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    32581 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    32641 tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    32701 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    32761 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    32821 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    32881 gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca gcgtaatcca
    32941 cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt tgcaccgctg
    33001 atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    33061 gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcccgttca
    33121 ggcgaaagcg tcgaagtgcg cgaccggcta gcgctcgacg gtaaaaaagc aggcggctat
    33181 tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc
    33241 ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    33301 attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac ccgcacgctg
    33361 ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    33421 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    33481 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    33541 gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt tctctggcgc
    33601 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    33661 cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc
    33721 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    33781 atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc cgaagcgtcc
    33841 ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc aggctcggcc
    33901 tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa tgccgacctt
    33961 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    34021 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    34081 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    34141 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    34201 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    34261 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    34321 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    34381 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    34441 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    34501 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    34561 gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    34621 taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    34681 gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag aaatttatgg
    34741 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaac
//
LOCUS       DAZOXR010000023        34220 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZOXR010000023 DAZOXR010000000
VERSION     DAZOXR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.023"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..114)
                     /locus_tag="ACHUNN_000959"
     CDS             complement(<1..114)
                     /locus_tag="ACHUNN_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575410.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVT"
     gene            complement(343..1263)
                     /gene="malM"
                     /locus_tag="ACHUNN_000960"
     CDS             complement(343..1263)
                     /gene="malM"
                     /locus_tag="ACHUNN_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0575411.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1415..2755)
                     /gene="lamB"
                     /locus_tag="ACHUNN_000961"
     CDS             complement(1415..2755)
                     /gene="lamB"
                     /locus_tag="ACHUNN_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0575412.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2827..3942)
                     /gene="malK"
                     /locus_tag="ACHUNN_000962"
     CDS             complement(2827..3942)
                     /gene="malK"
                     /locus_tag="ACHUNN_000962"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0575413.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4307..5497
                     /gene="malE"
                     /locus_tag="ACHUNN_000963"
     CDS             4307..5497
                     /gene="malE"
                     /locus_tag="ACHUNN_000963"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0575414.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5651..7195
                     /gene="malF"
                     /locus_tag="ACHUNN_000964"
     CDS             5651..7195
                     /gene="malF"
                     /locus_tag="ACHUNN_000964"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0575415.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7210..8100
                     /gene="malG"
                     /locus_tag="ACHUNN_000965"
     CDS             7210..8100
                     /gene="malG"
                     /locus_tag="ACHUNN_000965"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0575416.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8472..9947
                     /gene="xylE"
                     /locus_tag="ACHUNN_000966"
     CDS             8472..9947
                     /gene="xylE"
                     /locus_tag="ACHUNN_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0575417.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(9991..10401)
                     /gene="psiE"
                     /locus_tag="ACHUNN_000967"
     CDS             complement(9991..10401)
                     /gene="psiE"
                     /locus_tag="ACHUNN_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0575418.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10527..10670)
                     /locus_tag="ACHUNN_000968"
     CDS             complement(10527..10670)
                     /locus_tag="ACHUNN_000968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575419.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            10671..10949
                     /locus_tag="ACHUNN_000969"
     CDS             10671..10949
                     /locus_tag="ACHUNN_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575420.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(10996..13092)
                     /locus_tag="ACHUNN_000970"
     CDS             complement(10996..13092)
                     /locus_tag="ACHUNN_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0575421.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13092..13829)
                     /locus_tag="ACHUNN_000971"
     CDS             complement(13092..13829)
                     /locus_tag="ACHUNN_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0575422.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13826..14464)
                     /locus_tag="ACHUNN_000972"
     CDS             complement(13826..14464)
                     /locus_tag="ACHUNN_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0575423.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14578..14820)
                     /gene="yjbE"
                     /locus_tag="ACHUNN_000973"
     CDS             complement(14578..14820)
                     /gene="yjbE"
                     /locus_tag="ACHUNN_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0575424.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15282..16931)
                     /gene="pgi"
                     /locus_tag="ACHUNN_000974"
     CDS             complement(15282..16931)
                     /gene="pgi"
                     /locus_tag="ACHUNN_000974"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0575425.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17168..17364
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17456..18805
                     /gene="lysC"
                     /locus_tag="ACHUNN_000975"
     CDS             17456..18805
                     /gene="lysC"
                     /locus_tag="ACHUNN_000975"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0575426.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19057..19329
                     /locus_tag="ACHUNN_000976"
     CDS             19057..19329
                     /locus_tag="ACHUNN_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0575427.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19462..20334)
                     /gene="rluF"
                     /locus_tag="ACHUNN_000977"
     CDS             complement(19462..20334)
                     /gene="rluF"
                     /locus_tag="ACHUNN_000977"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0575428.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            20682..21519
                     /locus_tag="ACHUNN_000978"
                     /pseudo
     CDS             20682..21519
                     /locus_tag="ACHUNN_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            21589..22395
                     /locus_tag="ACHUNN_000979"
     CDS             21589..22395
                     /locus_tag="ACHUNN_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0575429.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22405..22812
                     /locus_tag="ACHUNN_000980"
     CDS             22405..22812
                     /locus_tag="ACHUNN_000980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0575430.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            22812..23306
                     /locus_tag="ACHUNN_000981"
                     /pseudo
     CDS             22812..23306
                     /locus_tag="ACHUNN_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23372..24169
                     /locus_tag="ACHUNN_000982"
     CDS             23372..24169
                     /locus_tag="ACHUNN_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0575431.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24180..25004
                     /locus_tag="ACHUNN_000983"
     CDS             24180..25004
                     /locus_tag="ACHUNN_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR0575432.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25056..26284
                     /gene="sorE"
                     /locus_tag="ACHUNN_000984"
                     /pseudo
     CDS             25056..26284
                     /gene="sorE"
                     /locus_tag="ACHUNN_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            26667..27357
                     /gene="pepE"
                     /locus_tag="ACHUNN_000985"
                     /pseudo
     CDS             26667..27357
                     /gene="pepE"
                     /locus_tag="ACHUNN_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27448..29079)
                     /locus_tag="ACHUNN_000986"
     CDS             complement(27448..29079)
                     /locus_tag="ACHUNN_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0575433.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29299..32982)
                     /gene="metH"
                     /locus_tag="ACHUNN_000987"
     CDS             complement(29299..32982)
                     /gene="metH"
                     /locus_tag="ACHUNN_000987"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0575434.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33182..34006
                     /gene="iclR"
                     /locus_tag="ACHUNN_000988"
     CDS             33182..34006
                     /gene="iclR"
                     /locus_tag="ACHUNN_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0575435.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         8049 a         8950 c         8857 g         8364 t
ORIGIN      
        1 tgtcacccca ttgaaattac gtagtaaatc aagatctact atattatttc cttgaaccac
       61 atcatgtttc atcaaagagt tattgccgcc agaatttgtg atatgaccta tcataaaacc
      121 tctgtaatac agaaataaaa taacctggaa aagtagcctg agcgtccaga cactaccggc
      181 gaaaggaaaa gaatctctga taaggcattg agataatgga cattcttagt gaagaagaag
      241 tgaagtacta taaaaaatcg ctcatatccc aggaaggaag cataaaaaaa ggtgcgccag
      301 gagacgcacc agttgttgca aaatcagcac tgtggggcgt aattacccct tgccttttac
      361 actgctgata aaggtggaac gggcagatgt cgatcccagg cgttcagctt catcaagcag
      421 ttttaacgcc ttatcaacat cacctttcgc gacagcgttt ttgatcgcgg tattaaaata
      481 actttccgtg tcgttgagca tcggctcgct tttcttcacc ggcaccggag caggtgcagc
      541 cacagctggt gccgccgtgt tgcctaccgt aaccggagct ggagcggaag aaccaaatag
      601 tggtcctacc aacacgctgg agctggagtt cgttttgact ttcagtttca gtaagccatc
      661 gctggtatga cgagcaaccg gatcgggaat atccgggatc gagttaccga cgcctttggc
      721 ataggcttta gccgggtcga gcagttgggt cgtctgctgg agatcttttt ccgtggtaaa
      781 gaccagaaca taaagttttt gctgccccaa cgccggtgtc aggcgcataa cgccttccag
      841 ccgatctgca ctcatcacgc ctggttcctg gtaggtgaaa taactgctgg ggaagaaggc
      901 tgatggggtc atgttctgat caagaatcag cacgttcggc gcaaaaacgc tggtttgttt
      961 gttcacttcg ctggtcagcg tcagggtcag ttcgccaatg tttgccggga cgctgtacgc
     1021 agcaaccgga ccactgatgc cgggaacgtt cagttgttgg ccgccggtcg ccagttgggt
     1081 ggtctgggtt ttagattgat cgaccggtgt ccaggtgagt tgttgcagcg cggcagatgg
     1141 aatggctggc gcgtcgctgg tgttttgcgg tacgtagcta acatcggcaa ggctaattcc
     1201 aggcgcgctt gccagtaacc ctgctgataa acagagggcg atgagacttt tattcatttt
     1261 cattgttttc acctcaaaat ctggagctca gcggtagcca ggcaatagcg cgctaaaccc
     1321 gataatcaga ggggctttcg cccctttcag ataatgacaa cctgttttta tgccggatgc
     1381 ggcgtaaacg ccttatccgg cccaggtttt gctattacca ccagatttcc atctgggcac
     1441 cgaaggtcca ctcgtcgctg tcgccacgac cgaagctgcc gccgttgaaa tcagcaggaa
     1501 cggctttgcc gtagttcggg ttaaccttgc tatcgccgtt gtagtcgtaa ccccatttct
     1561 catcccactt ggcgtaggtt gcgaagacac gaatagccgg gcgtgaccag atgctgtcgc
     1621 cagcctgcca ttgttgtgcg agggtaattt tgtactgatt gttcttgtcg ccggtgcgct
     1681 gggattcgac gttgtcgtag ccgatttcca tcacggtgct catgattggc gtccacttgt
     1741 acatcgggcg aataccgacg gtccaccact tggtgccgtt gtcgttatcc cagttgatat
     1801 cctggtacat acccacgtac atcatgtccc agttgtcgcc catggagatc gcaccgtggt
     1861 cgaggatacg cagcatgtga ccgttgttgt tgatattgta ggcaaatttt tcgttatcaa
     1921 acgcgacgcc agaaccctgc gacagacctt taccctgcga ggtcatcgag tcagtagcgt
     1981 actgaacaac aaacttgtta aagcccttca ggacactctg agtatgttca gcagtgaata
     2041 accagccgtc tttcgatgcg ccatcaacca gacgatagtt atcacgcagg ttggcacgac
     2101 cgtagtcgac acccagttct aatgtgccgc ccgggttgat ttccatctgc gctaaacgca
     2161 catcgaaaac gtcgttcgcg gtttcgttgg tatagtcata aatattgttg ctggcaaaag
     2221 aggaagaacc accagcttca gaggagcggg ttgctgccag agagagttta ccgaagccaa
     2281 catcgatgtt ttccagaccg gcaccaggac cagaaatatc ccagtagtag aagtcgatca
     2341 tatgaacgtc atgacgttgg tagaagcgct tacctgccca gatggtggaa cctggcagcc
     2401 attcgatcag gtttttaccc tgcacgtttg cttcacggaa ggccggatcg gtagcttccc
     2461 agtcattctg ttgcgcgacg gaataggcca cgttagtgtc gaaatagaag ctcttatcgc
     2521 cctctttcca cacttcctga cccaatttta attcagcata agtttcacat tcgttgccaa
     2581 gacggtattt actttgagca ccggtagtct ggaaacactg ttgttcaccg ccgctacctg
     2641 tccagccaat accggaacgt gcatagccgt ggaaatcaac agccattgcc tgagcagaca
     2701 ttacgcccgc tgcgacggca accgccagag gaagtttgcg cagagtaatc atcattctat
     2761 ctcctgagtc attgcttttc ttttttcaca tcacctgtga caggctttgt gtgttttgtg
     2821 gggtgcttaa acgcccggct ccttatgcag tcgacgacat gcagtgccat cctcacggaa
     2881 cagatggcaa cgctctggcg gcaggccgat agcgaatgtg gcaccttctt ctaccaacac
     2941 cacgtcgttc tggcggtaca ccaggttttg acgaatggaa gggatctgga tatggatttg
     3001 ggtttcgttg ccgagttgct cgacgacctg aacttcaccc tcaaggatga cgtcagcgat
     3061 atcactcggc agtagatgtt ccgggcgaat acccagcgac atattggctc caacctggac
     3121 atcacggctt tcaactggca gccagacttg ctgacgattt ggcatcggca gctccacctg
     3181 cacttgatcg attgcggtgg cggtcacttt taccggcagg aagttcatct ttggcgaacc
     3241 gataaatccg gcgacaaaac ggtctgccgg atagtggtac agctccagcg gtttcccaac
     3301 ctgcgccacg cgaccggcgt ccagcaccac gattttgtcg gccagcgtca tcgcttcgac
     3361 ctgatcgtgg gtgacgtaaa tcattgtgcg gcccaggcgt ttatgcagac gggagatttc
     3421 gatacgcatt tgcacacgca gtgcagcatc gaggttggag agcggttcat cgagcaaaaa
     3481 tacgcttggc tcggccacca gcgtacggcc aatcgccaca cgctgacgct gaccaccgga
     3541 gagcgctttc ggtttgcgat ccagcaaatg cgccagttgc agcacttccg ccacctggtt
     3601 gacgcgttgg ttaatcacct ctttttttgc gccagccagt ttcaggccaa atgacatgtt
     3661 ttctgctact gacaggtggg gatagagcgc gtaagactga aacaccatac caacgccgcg
     3721 ttctgctggc ggagtgtcat tcatccgttt ctcaccgatg aacaggtcgc cgctggtgat
     3781 cgtctcaagc ccggcaatca tgcgcagtaa agtcgattta ccgcagccag acggtccgac
     3841 aaacaccacg aattcacctt catggatatc gagattgata tctttcgata ccacgacctc
     3901 gccccaggct ttcgttacat tttgcagctg tacgctcgcc atgcccttct ccctttgtaa
     3961 caacctgtca tcgacagcaa cattcatgat gggctgacta tgcgtcatca ggagatggct
     4021 taaatcctcc accccctggc ttttttatgg gggaggaggc gggaggatga gaacacggct
     4081 tctgtgaact aaaccgaggt catgtaagga atttcgtgat gttgcttgca aaaatcgtgg
     4141 cgattttatg tgcgcatctc cacattaccg ccaattctgt aacagagatc acacaaagca
     4201 acggtggggc gtaggggcaa ggaggatgga aagaggttgc cgtataaaga aactagagtc
     4261 cgtttaggtg ttttcacgag cacttcacca acaaggacca tagattatga aaataaaaac
     4321 aggtgcacgc atcctcgcat tatccgcatt aacgacgatg atgttttccg cctcggctct
     4381 cgccaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
     4441 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
     4501 tccggataaa ctggaagaga aattccctca ggttgcggca actggcgatg gccctgacat
     4561 tatcttctgg gcgcacgacc gttttggtgg ctacgctcaa tctggcctgt tggctgaaat
     4621 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
     4681 caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa
     4741 agatctgctg ccgaacccac caaaaacctg ggaagagatc ccggcgctgg ataaagaact
     4801 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
     4861 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgatattaa
     4921 agacgtggcc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgaccttat
     4981 taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa
     5041 aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg acaccagcaa
     5101 agtgaattat ggtgtaacgg tactgccgac cttcaagggt caaccatcta aaccgttcgt
     5161 tggcgtgctg agcgccggta ttaacgccgc cagtccgaac aaagagctgg cgaaagagtt
     5221 cctcgaaaac tatctgctga ctgacgaagg tctggaagcg gtcaataaag acaaaccgct
     5281 gggtgcagtg gcgctgaagt cttacgagga agagttggcg aaagatccac gtattgccgc
     5341 caccatggaa aacgcccaga aaggtgaaat catgccgaac atcccgcaga tgtccgcttt
     5401 ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga ctgtcgatga
     5461 agccctgaaa gacgcgcaga ctcgtatcac caagtaatgc tgtgaaatgc cggatgcggc
     5521 gtgaacgcct tgtccggcct acaaaactga aacgcatgta ggcctgataa gacgcatcag
     5581 tgtcgcatca ggcagttgtt gtcggataag gcgtgaaagc cttatccgtc ctggaatgag
     5641 gaagaacccc atggatgtca ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg
     5701 gtcagtgcta ggtctgctcg gcctgctggt gggttacctt gttgttttaa tgtacgcaca
     5761 aggggaatac ctgttcgcca ttaccacgct gatattgagt tcagcggggc tgtatatttt
     5821 cgccaatcgt aaagcctacg cctggcgcta tgtttacccg ggaatggctg gaatgggatt
     5881 attcgtcctc ttccctctgg tctgcaccat cgccattgcc ttcaccaact acagcagcac
     5941 taaccagctg acttttgaat gtgcgcagga agtgttgtta gatcgctcct ggcaagcagg
     6001 caaaacctat aactttggtc tttacccggc gggcgatgag tggcaactgg cgctcagtga
     6061 cggcgaaatc ggcaaaaatt acctctccga cgcgtttaaa tttggcggcg aacaaaaact
     6121 gcaactgaaa gaaacgaccg cccagcccga aggcgaacgc gcgaatctac gcgtgattac
     6181 ccagaatcgt caggcgctga gtgacattac cgccattctg ccggatggca acaaagtgat
     6241 gatgagctcc ctgcgccagt tttctggcac gcagccgctc tacacactcg acggtgacgg
     6301 cacgttgacg aataatcaga gcggcgtgaa atatcgtccg aataaccaaa ttggctttta
     6361 ccagtccatt accgccgacg gcaactgggg tgatgaaaag ctaagccccg gttacaccgt
     6421 gactactggc tggaaaaact ttacccgcgt cttcaccgac gaaggcattc agaaaccgtt
     6481 cctcgccatt ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt taacggtggc
     6541 ggtcggcatg gttctggcat gtctggtgca gtgggaagca ttgcgcggca aagcggtcta
     6601 tcgcgttctg ctgattctgc cctacgcggt gccatcattc atttcaatct tgattttcaa
     6661 agggttgttt aaccagagct tcggtgaaat caacatgatg ttgagcgcgc tgtttggcgt
     6721 gaagcccgcc tggttcagcg atccgaccac cgcccgcacg atgctaatta tcgtcaatac
     6781 ctggctgggt tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag cgattccgga
     6841 cgatttgtat gaagcctcag caatggatgg cgcagatccg ttccagaact tctttaagat
     6901 tacgctgccg ctgctgatta aaccgctgac gccgctgatg atcgccagct tcgcctttaa
     6961 ctttaacaac ttcgtgctga ttcaactgtt aaccaacggc ggcccggatc gtcttggcac
     7021 gaccacgcca gccggttata ccgacctgct tgttaactac acctaccgta tcgcttttga
     7081 aggcggcggg ggtcaggact tcggtctggc tgcagcaatt gccacgctgc tcttcctgct
     7141 ggtgggtgcg ctggcgatag tgaacctgaa agccacgcga atgaagtttg attaagggag
     7201 ataacaaaaa tggcaatggt ccaaccgaaa tcgcaaaaag cacgtttgtt tattactcac
     7261 ctgctactgc tactttttat cgcggcgatt atgttcccgc tgctgatggt cgtcgctatc
     7321 tcactgcgcc aggggaactt tgcgatcggc agcctgatcc cggagcaaat ctcctgggat
     7381 cactggaaac tggctttagg ttttagcgtg gaacaggctg atggtcgcat tacgccaccg
     7441 ccattcccgg tactgctgtg gctatggaac tcggtaaagg tcgccgggat ttccgcgatt
     7501 ggcattgtcg cgctctctac cacctgcgcc tacgctttcg cccgtatgcg ctttccaggc
     7561 aaagcgacgc tgctgaaagg aatgctgatt ttccagatgt tcccggcagt actttcactg
     7621 gtcgcgttgt atgcgttgtt tgatcgtctg ggtgagtaca ttccattcat tggcctgaat
     7681 actcacggcg gcgtaatttt cgcgtatctg ggtgggattg cgctgcatgt ctggaccatc
     7741 aaaggctatt tcgaaaccat cgatagttcg ctggaagaag cggctgcgct ggatggtgcg
     7801 acaccgtggc aggccttccg ccttgtcctg ttgccgctgt cagtaccgat tctggcggtg
     7861 gtattcatcc tgtcgtttat cgctgccatt actgaagttc cggtcgcgtc gctgttactg
     7921 cgtgacgtaa acagctacac cctggccgtg gggatgcagc aatacctcaa cccgcaaaac
     7981 tacctgtggg gtgactttgc cgccgctgcc gtgatgtctg cattaccgat caccatcgtc
     8041 ttcttgctgg ctcaacgctg gctggtcaac ggcctgacgg caggtggtgt gaaaggttaa
     8101 agatgttgtt ctgccaatgt tatgccgctg caccctcaac ttacgttatc ccaacttgtg
     8161 actgttattc ggcgctccac ggagcgcctt tttttctttc gtctgcaatc tgaatcgttc
     8221 gccggttaat atttccatca tagagcttat tatttttacg ttatttgttt tcccacttac
     8281 gataattctc tttcgtgctc tgagtcacgg caatagtatt gttttcatca atttttgata
     8341 attatcacaa ttaagatcac agaaaagaca ttacgtaaac gcattgtaaa aaatgataat
     8401 tgccttaact gcctgacaat tccaacatca atgcactgat aaaagatcag aatggtctaa
     8461 ggcaggtctg aatgaatacc cagtataatt ccagttatat attttcgatt accttagtcg
     8521 ctacattagg tggtttatta tttggctacg acaccgccgt tatttccggt actgttgagt
     8581 cactcaatac cgtctttgtt gctccacaaa acttaagtga atccgctgcc aactccctgt
     8641 taggattttg cgtagccagc gctctgattg gttgcatcat cggcggtgcc ctcggtggtt
     8701 attgcagtaa ccgcttcggc cgtcgtgatt cacttaagat tgctgctgtc ctgtttttta
     8761 tttctggtgt aggttctgcc tggccagaac ttggttttac ctctataaac ccggacaaca
     8821 cagtacctat ttatctggca ggttatgtcc cggaatttgt tatttatcgt attattggcg
     8881 gtattggcgt tggtttagcc tcaatgcttt caccaatgta tattgcggaa ctggctccag
     8941 ctcatattcg cggaaaactg gtttcattta accagtttgc gattattttc gggcaacttt
     9001 tagtttactg cgtaaactat tttattgccc gttccggtga tgccagctgg ctgaatactg
     9061 acggctggcg ttatatgttt gcctcggaat gtatccctgc actgctgttc ttaatgctgc
     9121 tgtataccgt gccagaaagt cctcgctggc tgatgtcacg cggcaagcaa gaacaggcgg
     9181 aaagtatcct gcgcaaaatt atgggcaaca cgcttgcaac tcaggcagta caggaaatta
     9241 aacactccct ggatcatggc cgcaaaaccg gtggtcgtct gctgatgttt ggcgtgggcg
     9301 tgattgtcat cggcgttatg ctctccatct tccagcaatt tgtcggcatc aatgtggtgc
     9361 tgtactacgc gccggaagtg ttcaaaacgc tgggggccag cacggatatc gcgctgttgc
     9421 agaccattat tgtcggagtt atcaacctca ccttcaccgt tctggcaatt atgacggtgg
     9481 ataaatttgg tcgtaagcca ctgcaaatta tcggcgcact cggaatggca atcggtatgt
     9541 ttagcctcgg taccgcgttt tacactcagg caccgggtat tgtggcgcta ctgtcgatgc
     9601 tgttctatgt tgctgccttt gccatgtcct ggggtccggt atgctgggta ccgctgtcgg
     9661 aaatcttccc gaatgctatt cgtggtaaag cgctggcaat cgcggtggcg gcccagtggc
     9721 tggcgaacta cttcgtctcc tggaccttcc cgatgatgga caaaaactcc tggctggtgg
     9781 cccatttcca caacggtttc tcctactgga tttacgggtg tatgggcgtt ctggcagcac
     9841 tatttatgtg gaaatttgtc ccggaaacca aaggtaaaac ccttgaggag ctggaagccc
     9901 tctgggaacc ggaaacgaag aaaacacaac aaactgctac gctgtaatct tcctgtccag
     9961 cacgccgcgc cagttcggcg tgctttcttt ttactcccgc ttcagccgtt tcgaattaca
    10021 cagccacaga gtgatcacca gtaacaggat cgcagccgag tagatcaaca catccagtgg
    10081 cgatttatga tcgacgatga tcaagcgcac aatggcggtg atcccaatat agacaaagta
    10141 acgtagcggg aagtgaaaac cggactgaaa gtacttcaca atcagcgcga taaattcgaa
    10201 atagagaaag taaaccacca gtccttctac cagctcatat ttgctggttt gttctggcgc
    10261 gaacagcaca tctgccagat gcaccgtttc tttgccgagg aagacaacca aaatcaggcc
    10321 gaggcacagc aggccaagat tgagtacggt ctgcaaaatg gtggagataa actccacgcg
    10381 cggacgagag agtgacgtca tagcagtacc tccttcacag tggcgagctt cctgtataac
    10441 gcaaaaatgt gacggagatc tatatttttt gtgtatgttt tgttcaacct ggatttggag
    10501 cgcgcctcca ccaaagccgg atatacctac ccgtaggccg gataagccgc gcctggcggc
    10561 gcatccggca agacaaaccg cacccaagcc ggtaattcaa aaatcaccca ttcaggtgat
    10621 tcccagccac cagcaatgcg cgaaaaatcg acaatgttcc tctggaacaa atgaaacgta
    10681 acctgataaa ggtggtaaaa atgaagcctt attttgctgc tttgatgtta tcagtctctg
    10741 ttcttcctgc ttatgctggt ccgctcggta ctgctgataa agccgaccta ccgcaaagca
    10801 atgtctccag cccaatgatg gcccagtcgc tcaggcaatc agacctgcaa ccgatatcga
    10861 cggacaggaa gacagaatgt tttcgccttt acacaccaga tcgcaaaccg ggggtgaact
    10921 gcgttcctga tggctcaaca ggccattaag aatgagggtg ccagtgattg cgaaaactgg
    10981 cacctttccc atgacttagc ggaaattgac gctacggtcg ctggtcatat catacaactg
    11041 gaacttacgc ccaagttgct gaccaccgtc acgcgtcagc ggcgtccagc caatcgccgc
    11101 acggctgcgt gtcgggccag acgagaagag atccaacggt accgagacat acacgccttt
    11161 ggtgaagtcc ccttcgccgt actcctcttt tgaaacattc gtgatcgtgg catagccacc
    11221 caccaccacg ccgctatcaa agcgtttggc gatctccagc gtgccgcctt tatcccctgc
    11281 cagatactgc ccgacgctgg ctttcactaa cacgtcctgg gcgaaagatg gcgtccagta
    11341 ggcggtcaga tgtccggttt tcacgctgta gtcggtgaat ttcatcatat cttttgcact
    11401 acgccagtcg cgctgtttaa cgtagttggc atccagacca aacgcccagt tgctgtccag
    11461 cggacgatac aacacttcag cccccgcacc gccaaacatg gtttcgagat aacctccata
    11521 gacctgaccg tagaagccgt tgcccagatg ctggaagtag ttggcttgca ggttattcac
    11581 ataggcatcg ttctgcacat actcgcgcac atgggtacga acgcgcggta agtgcgagtc
    11641 ctgtggcgga ttggtgtagt taaacttgtc gtagttgttg gcaagatttg caaacaggct
    11701 gccgatggtc agcagatggt ccgtcagcca caaatctgcc gttcccatca cgcccagctg
    11761 atacatgtaa aagttttccg ggccaccgac cgactggttc agcaccggat cgatatggaa
    11821 atcaaagcgt gatttgtcga tataccagcc ctgctcggtg gactgcggaa ccactggctc
    11881 gacgcgtttt tgcgccagcg tcgtttcgtg gcccaacggc tctccggcga gatgattttt
    11941 caggctggcg acatcggttt ccgtcgtcac ctgcggcatg ttaaggcgat tttccgtaat
    12001 gcggatcgtt ttgatcccat ccggcagatc gttcatcacg atccgattag cacggatgat
    12061 cccttcgcgc gaatcacgat atttcacctg ctcgccggta acatacagcg tatcgccttt
    12121 cgcctggatc tgcggatcgg caagtccggc attgtatttc agcaacgtta actgattcgc
    12181 caccaccgag tgctgcaaaa tggcatcctg cggctgcggt tgatattgcg ggcgggcgtt
    12241 atcgatgtag gacgggcgca gatcgttaaa gttggtgcgc agcgtgacgc caaacataaa
    12301 ggtgttgcca cgttcatagc taaggttaac gtcggcccaa tcggtaacgc gataaatcgc
    12361 cccgacgtta aacttacttt tttgctccag cttcccggca aaatcctgct gataattatt
    12421 accttcatac tccagtttca ggcgcagcgg ttgccagggc gtctggtatt ccacgccgcc
    12481 aaacagtgag gccgggccgt ggaacatctg actgccgtcg atagatcccg cttgtttgta
    12541 gctgttatcg cgatagcaat atttatcact ggctgaacag agcggatttt tcacattacc
    12601 gctggtgccc agatatcccc agcccaggcc gagcgtaaaa tcgaacggcc cccaggcttt
    12661 gctggcaaca agatattccg catcaaacag ccccgtaccg ccgatatccc gcgcaccaac
    12721 cgccacttgc ggcagccagt aactctcttc ccacaaacgc agtttgagat cgaaggcttt
    12781 atctttatac gtttgatcgc cagagaacgc ttcgacgctg ctgtactgcc gggtgcgcac
    12841 gtcggtgtaa cgcagcgttg tttccagcca cgggaagagt tgcactgaag ctgagtaata
    12901 acggtactga tcgttatcgc gatagttcag actcaactcc ccttcccgtg ccatacgcgc
    12961 ggtgggagtt tgtaataatc ctacgccacc gaaatccgac tgcgacggac caatgggcgc
    13021 aggatatgtt tcgccgtagc aagctgtgct aatgcccagc gccagtaagc taagcagatg
    13081 tctttttttc attattgggg tatccgctgc gtcagagtct gaagaatgtc ggcgttaagg
    13141 gcatcaggcg tctcactcca gacggagtct gcgaggccaa cataaataat gctgccgggc
    13201 atcggctcta cgtgacgctt gttccagtaa gccaccggcg ctttttgcgt gcgtccgtcc
    13261 gggtaaacga cccacgcgta gctgcgatcc gcaccgctga gcaggctctg gtcagagaga
    13321 tagctcgcca cgtcgcgacc tggagtgaat ggctgattgc caggatggct gataagcccg
    13381 aacaacgtga ccgtggacgg cggtggtccg acccacagcg tgtagttgcc ctgcaacggc
    13441 gggttaccgc gttcggcaac gcggacgata tcgggatcaa gattgatttt ttgcctgcct
    13501 gtcacttcca atgcctggat ttgctggcgt aaggcgttaa tggcggcggc atcgtcggtg
    13561 ctggaatctg cgccctgttc tgccagccgt gtcagcaacg cctgctgctg acgcaatgct
    13621 gccgccgttg ccagctcttc gctaatcacc gcaccaggcc accagctgtt tgccagtcga
    13681 ggttgcccta ccagatcgat taaatgctct gcgcccgtta gcgttttggc ctcactgctg
    13741 ccattgctga acaccttgac ggttcctgcc gcgaagaccg atgacgctcc cacactcaaa
    13801 agcaacgcga caatagtttg tttaatcatt gttttgccgc cttgatgaga gtggttttca
    13861 ccgggaaata atccgcgccg agatactgtt ccgactggcg aatttgccct tcgctgtcga
    13921 tccagtagcg gttatgccag cgcgcctggt cggtggagac ttcttcgtcc agcacgcgaa
    13981 ccagggtttc atcgctgccg accttgacgg tatcggtgcc atcccatttg aagactgagc
    14041 gtgcggtggc gtagcgtacc tgctggtact cggtccagcc catcgtgcgc gtccatgttg
    14101 caccatcaac aatttgtgcg ggtttaatca gcgggtcggc ggcaaggtta ttcacttcaa
    14161 tcaggttgtc gccgccaagc aaagtcttca ccagacgacc atgttgtgtg acaagagtgg
    14221 cttgatcctg agtgacccat ttttgttgtc cgtcttcagc gaaggccagc accacaaaca
    14281 attgcggccc gccattaagc tgcatgtact ggctggcgta gggcatattt tgaatatcat
    14341 cgtccgtcag ctgtacgcct ggcgtgccga acagactgtc ccacagtgaa ttgcccagct
    14401 ctttagtcgt ggctgaacag gcctgtaata acaggcagat aagaatgagt gcaggtcgct
    14461 tcacgactct tctccagagg ggcagaataa ccacaccgga gtgtggttat ggttaatccc
    14521 ttcatatttc aagccacaga tgcaatgact cgaattattt tggggataca tactttatta
    14581 ctgggtactg gtggttgtgg ttgttgtggt cccggtatta gaaccatcac cgccaccggt
    14641 tgccgcgagc gcgacaccca ccgccgagct tacggtgctg acgctggtcg cggaggaact
    14701 ccccgccgaa accgacgttg ctgccgcccc tgccgcttcg cccacctgta caggtgcagc
    14761 ccacacagaa gtcgccgcaa gcgcagatat ggcaaaaatg ccatacagaa cttttttcat
    14821 aacaatttcc cttcattgaa tgaatggaga tttacccaaa aacatttcgg gcgggatcga
    14881 gtatacacaa ctaaagcatg cgaattacgg cgcggggaat tagcgatggt gttttaggat
    14941 aattggaatg aatatcaaat ataagtttca acttatattt aaataacaat aaaaatcata
    15001 aacttaaaat aaaaacaaaa gtaattaatc tgcgtattct ccgaaataaa caacgattaa
    15061 aaatctattt taggattaac ctcacggtat gatttccgtt aaatcacaga caaggggatt
    15121 tatctgataa aacaatgccg acttattgtc ggcattgttt ttaaggttat ttaactcctc
    15181 atactatgca ttaatgtcga tattggcatc aggctccggt tgccggatgc ggcgtaaacg
    15241 ccttatccgg cctacatatc ggcatcaggc acgcatgatg attaaccgcg ccacgcttta
    15301 tagcggttaa tcagaccatt ggtcgagcta tcgtggctgc tgatttcttt gtcatctttc
    15361 agctctggca gaatacggtt cgccagttgt ttacccaact ccacgcccca ctggtcgaag
    15421 gtaaagatgt tcaggatcac gccctgagta aagattttgt gctcatacag cgcaatcaac
    15481 gcacccaggc tgaacggagt gatttcgcgc agcaggatgg agttggtcgg gcggttacct
    15541 tcgaacactt tgaacggcac cacgtagtca agcgttgccg gatctttacc ctgatcgcga
    15601 tattcctgct caaccacttc gcgggattta ccaaacgcca gcgcttcggt ctgggcgaag
    15661 aagttagaca gcagtttctg gtggtgatca gagagcgggt tatgggtgat agccggggcg
    15721 atgaaatcgc acggtaccat tttggttccc tggtggatca gctggtagaa cgcgtgctga
    15781 ccgttagtgc ctggttcacc ccagataatc gggccagtct ggtaatccac aacgttaccg
    15841 ttacggtcaa catacttacc gttggactcc atattgccct gctggaagta cgccgcgaaa
    15901 cggtgcatat actggtcata cggcagaatc gcttcagttt ccgcaccaaa gaaattgttg
    15961 taccagatgc caatcagcgc cagcaggaca ggcaggtttt tctcggcagg cgtggtggag
    16021 aaatgcttgt ccatcgcgtg tgcgccagaa agcagttcaa cgaagttatc aaagccgatg
    16081 gagagaacaa tcgacaggcc aatcgctgac cacaaagagt aacggccgcc gacccagtcc
    16141 cagaactcga acatgttggc agtatcaata ccaaactcgc caacggcttt ggcattggtg
    16201 gaaagcgccg caaagtgttt tgcaacgtgt ttttcatcac ctgccgcttt caggaaccag
    16261 tcacgcgcgc tatgggcgtt ggtcatagtt tcctgagtgg tgaaggtttt agatgctacc
    16321 aggaacagcg tggtttccgg gtttactttt ttcagcactt cagcgatgtg agtcccatcg
    16381 acgttagaaa caaagtgcat gttcaggtga tttttgtacg gacgcagtgc ttcggtcacc
    16441 atgtacgggc cgaggtcaga accaccgatc ccgatgttca ctacgtcagt gattgctttg
    16501 ccggtataac ccttccactc accggagata atcgcttccg agaaggtttt catcttctcc
    16561 agcaccgcgt tgacttccgg cattacgtct ttgccatcaa ccagaatcgg ggtattgcta
    16621 cggttacgca gcgctacgtg cagcacggcg cggttttcag tgcggttgat cttctcgcca
    16681 gagaacatcg acttaatcgc gcccgccaga tcgcactctt tcgccagatc ctgtaatttc
    16741 gccagcgtct cttcagtgat gcggtttttg gagtaatcca ccagcatctg atcgtcgaat
    16801 gttgcggaga acttagaaaa acgatcgccg tctttagcaa aaagatcggc gatcgtaacg
    16861 tctttcattt catcgaagtg tttctgtagt gcctgccagg cagcggtctg cgttggattg
    16921 atgtttttca ttagcaatac tcttctgatt ttgagaattg tgactttgga agattgtagc
    16981 gccagtcaca gaaaaatgtg atggttttag tgccgttagc gtaatgttga gtgtaaaccc
    17041 ttagcgcagt gaagcattta ttagctgaac tactgaccgc caggagtgga tgaaaaatcc
    17101 gcatgacccc gtcgttgaca accgccccgc tcacccttta tttataaatg tactacctgc
    17161 gctagcgcag gccagaagag gcgcgttgcc caagtaacgg tgttggagga gccagtcctg
    17221 tgataacacc tgagggggtg catcgccgag gtgattgaac ggctggccac gttcatcatc
    17281 ggctacaggg gctgaatccc ctgggttgtc accagaagcg ttcgcagtcg ggcgtttcgc
    17341 aagtggtgga gcacttctgg gtgaaaatag tagcgaagta tcgctctgcg cccacccgtc
    17401 ttccgctctt cccttgtgcc aaggctgaaa atggatcccc tgacacgagg tagttatgtc
    17461 tgaaattgtt gtctccaaat ttggcggtac cagcgtagct gattttgacg ccatgaaccg
    17521 cagcgctgat attgtgcttt ctgatgccaa cgtgcgttta gtagtcctct cggcttctgc
    17581 tggtatcact aatctgctgg tcgctttagc ggaagggctg gaacctggcg agcgattcga
    17641 aaaactcgac gctattcgca acatccagtt tgccattctg gaacgtctgc gttacccgaa
    17701 cgttatccgt gaagagattg aacgtctgct ggagaacatt actgttctgg cagaagcggc
    17761 ggcgctggca acgtctccgg cgctgacaga tgagctggtc agccacggcg agctgatgtc
    17821 gaccctgctg tttgtcgaaa tcctgcgcga acgcgatgtt caggcacagt ggtttgatgt
    17881 acgtaaagtg atgcgtacca acgaccgatt tggtcgtgca gagccagatg tagccgcgct
    17941 ggcggaactg gccgcgctgc agctgctccc acgcctcaat gaaggcttag tgatcaccca
    18001 gggatttatc ggtagcgaaa ataaaggtcg tacaacgacg cttggccgtg gaggcagcga
    18061 ttatacggca gccttgctgg cggaggcttt acacgcatct cgtgttgata tctggaccga
    18121 cgtcccgggc atctacacca ccgatccacg cgtagtttcc gcagcaaaac gcattgatga
    18181 aatcgcgttt gccgaagcgg cagagatggc aacttttggt gcaaaagtac tgcatccggc
    18241 aacgttgcta cccgcagtac gcagcgatat cccggtcttt gtcggctcca gcaaagaccc
    18301 acgcgcaggt ggtacgctgg tgtgcaataa aactgaaaat ccgccgctgt tccgcgcgct
    18361 ggcgcttcgt cgcaatcaga ctctgctcac tttgcacagc ctgaatatgc tgcattctcg
    18421 cggtttcctc gcggaagttt tcggcatcct cgcgcggcat aatatttcgg tagacttaat
    18481 caccacgtca gaagtgagcg tggcattaac ccttgatacc accggttcaa cctccactgg
    18541 cgatacgttg ctgacgcaat ctctgctgat ggagctttcc gcactgtgcc gggtggaagt
    18601 agaagaaggt ctggcgctgg tcgcgttgat tggcaatgac ctgtcaaaag cctgcggcgt
    18661 tggcaaagag gtattcggcg tactggaacc gttcaacatt cgcatgattt gttacggcgc
    18721 atccagccat aacctgtgct tcctggtgcc cggcgaagat gccgagcagg tggtgcaaaa
    18781 actgcatagt aatttgtttg agtaaatact gtatggcctg gaagctatat ttcgggccgt
    18841 attgattttc ttgtcactat gcttatcaat aaacgagcct gtactctgtt aaccaacgtc
    18901 tttatcgaga ataattgcct ttaatttttt tatctgcatc tctaattaat tatcgaaaga
    18961 gataaatagt taagagaagg caaaatgaat attatcagtt ctgctcgcaa acgaattccc
    19021 gtacactgaa ctctctggct cactgagggc accatcatgg cactcccccg tattacccaa
    19081 aaagagatga ctgaacgcga gcagcgtgaa cttaaaacac tgctggatcg ggcgcggatt
    19141 gcgcatggtc gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat
    19201 aaattaatgg tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag
    19261 gcttataaac cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc
    19321 aggcgctaat gaacaatcaa cgcctgagat tttcactcgt cagtgtgaac acaattttcc
    19381 cctcacccta acccttttcc cgaaggggcg aggggactgt ccgagcactt ttgggccgtt
    19441 ttcatcagtc tgaggcgtta atcagcgccc cttcttttta cgccccggcg aggtaaaacg
    19501 cttaccgttg gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg
    19561 acgtttgatg cccgctgttt tcggtttggc cttcgctttg ggcttcgcct cggaagagga
    19621 attttcaatg agcttaaaga gatcgataag ctcgtcgtcg gttaaatcgc gccattctcc
    19681 cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta cgttccagct ttttcacttc
    19741 atagccgaaa tgctcgcaca tgcgccggat ctgacggttc aacccctgca ccagggtaat
    19801 gcggaagaca aacggtgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat
    19861 cggcactccc gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac
    19921 cagatactct ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg
    19981 attggtgagg aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg
    20041 tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac
    20101 aataccgacg ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat
    20161 caactgaccg tttactttca caatgtcgcc gggtttcacc tgatcaccaa tggtggctcg
    20221 cttgccatta aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca
    20281 aattccgctt tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc
    20341 tcctgtaaaa cgcggaatat accgtacctc ttagcgggga aaaacagggt tatgcggcaa
    20401 cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa aagtgtctcc ggaagtacga
    20461 tgcaaatttg cctggcaaaa taaggataat caacatatta cgaatcgctg cacacctgtg
    20521 ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa gcgctatttc tccccatgca
    20581 aagagtgaaa aatcgctttg aaaggccctc ataatggggt tatatcagat caagcggtga
    20641 gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc aatggaaaac agtgacgata
    20701 tccgtttgat tgtgaagatt gcccaactct attacgaaca ggatatgacg caggcgcaaa
    20761 tcgcgcgcga actgggtatt taccgcacca ccatcagccg cttgcttaaa cgaggccgcg
    20821 atcagggaat tgtcaccatc gccatcaact atgactacaa cgaaaatctc tggctggagc
    20881 agcaactgaa gcaaaagttt ggcttgaaag acgttgtggt ggtgtcggga aatgatgagg
    20941 atgaagagac tcaactggcg atgatggggt tacacggcgc gcaactgctg gatcgcttgc
    21001 tggagcctgg cgatattgtc ggtttttcct agggtcgcgc ggtgagcgca ctggttgaaa
    21061 acttgccgca agcggggcaa tcgcggcagt taatctgcgt gccaattatt ggcggaccgt
    21121 ccggtaaact cgaaagccgc tatcacgtaa acacattaac ctacagcgtg gcagcgaagc
    21181 tgaaagggga atcgcatctc gcggattttc cggctctgct ggataacccc ttaattcgta
    21241 atgggatcat gcagtctcag cactttaaaa ccatctctgc ctactgggat aatctggatg
    21301 tcgccctggt gggaattggc tcaccggcca ttcgcgacgg cgctaactgg catgcgtttt
    21361 atggtggtga agagagtgac gacctgaaag caggcgcggt attccattgg cattgccatg
    21421 agtgaagaaa aatacagcgg aattgttggt gcactgcgtg gaaaatatat taattgtctg
    21481 gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg atgtttaccg
    21541 taataaaaat tacggcgggt ccctattatc taaaatcagg agtctgttat gcaaacgtgg
    21601 ttaaatttgc aggataaaat cattatcgtc accggcggcg catccggtat tggtctggcg
    21661 attgtagagg aattattagc acaaggcgca aacgtacaga tggtagatat tcacggtggc
    21721 gatggtcaat atgaagttca taaaggctat cagttttggc cgaccgatat ttccagcgcc
    21781 aaagaggtaa atcatacggt agcggaaatt atccagcgtt ttggtcgcat cgacggtctg
    21841 gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc gcctgccggg
    21901 cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca aaaaggcgtt
    21961 tttctgatgt cgcaggcggt ggcgcgccag atggtcaaac aacatgatgg cgtgattgtg
    22021 aatgtttcct cagaaagtgg actggaaggc tcagaaggcc aaagctgtta cgccgctacc
    22081 aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag agctgggtaa acacggtatc
    22141 cgtgtggtcg gtatcgcgcc ggggattctg gaaaaaaccg ggctacgcac gccggaatat
    22201 gaagaggcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga aggctatacc
    22261 aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtcgctga ttttgtttgt
    22321 tatctgctgt ctgaacgcgc cagctacatc accggagtaa ccactaacat tgcgggcggc
    22381 aaaacgcgcg gataaggagg ctttatggtc aacgccattt tttgtgccca cggcaaatta
    22441 gcttgcgcca tgctggaatc ggttcagatg gtgtacggcg acgccaatgt cgaggcggtt
    22501 gaatttgtac caggcgagaa cgccggagac atcgtagcaa agctggaaaa gttagtaagc
    22561 attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag cccgtggaat
    22621 gcggcagcga tgcttgcgat gcgcaatcct cgtctacggg taatcagcgg cctttccctg
    22681 ccgctggcgc tcgaactggt tgataaccag gacagcatga atgttgatga actgtgtgaa
    22741 cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc aacggcagag
    22801 gaagatttct gatgaacatt acgctcgccc gcattgatga ccgtctgatc cacggccagg
    22861 tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat tattatctgc aatgacgaag
    22921 tttataacga tgaagtgcgc cggacattat tgcgtcaggc tgcgccaccg ggaatgaagg
    22981 ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa tattaggacg
    23041 aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt cgccaggggg
    23101 taaaaatagg caccctgaat attggcggca tggcctggcg gcccggtaaa aaacagttaa
    23161 ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta aataatcttg
    23221 gcgtcataat ggatttacgc gtcgtagcgt cagatccctc aatcaatatt atcgacaaaa
    23281 taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc tttaacaggc
    23341 agggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac aaattattgc
    23401 tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat ttcagactca
    23461 tcgcccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa aaaccggaat
    23521 aatactcggt ggtacgctgg aattgatagc tctcggctgg atgaacgtcg gcgcggcgca
    23581 atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg ttggtcagca
    23641 aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc aagtgctgac
    23701 ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag cagcggaaga
    23761 ggcgcggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc aggcgctgcg
    23821 cgttgctatt ccggcactga ttgtctcact gttcgtcagc gccgatatgg tcagcaatat
    23881 gttaaacgcc attcccgaat ttgtgacccg tggactgcaa attgctggcg gttttatcgt
    23941 ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg aaatatttga tgcctttctt
    24001 tttcctcggt ttcctcgcag gtggctacct cgatctcagc ctgctggcgt tcggtggcgt
    24061 cggcgtgatc atggccctgg tctacatcca gctaaatcca cagtggcgta aagctgaacc
    24121 tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac ggagccatca
    24181 tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc tccaacctgc
    24241 aacaggcgtc ctttaacttt gaacgtattc acggactggg cttttgctac gacatgatcc
    24301 ccgccatcaa gcgactttac ccattaaaag aggatcaggt tgcggcgctc aggcgacacc
    24361 tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgtc actgccgcca
    24421 tggaagaggc gcgggccaac ggcgcggaaa ttgatgacgg taccattaac ggcatcaaag
    24481 tcggtctgat gggaccattg gcaggagttg gcgatccact ggtctgggga acgctgcgcc
    24541 cgattaccgc tgcgctcggc gcatctctgg cactttctgg caacatcctc ggcccgctgc
    24601 tgtttttctt tattttcaat gcggtgcgtc tggcgatgaa gtggtatggc ctacagctcg
    24661 gctttcgcaa aggggtgaat atcgtcagcg atatgggcgg gaatgtgctg caaaaactca
    24721 ccgaaggcgc gtcgattctc ggactgtttg tgatgggcgt gctggtcacc aaatggacgt
    24781 caatcaacgt accgttggtg gtttcacaaa cgcatgccgc cgatggctcc accgtcatca
    24841 tgaccgtgca gaacattctc gaccaacttt gccctggttt gctggcgctc ggtctgacgc
    24901 tactgatggt tcgtctgctc aacaaaaaaa ttaacccggt atggctgatt ttcgccctgt
    24961 ttggcttagg gattatcggc aatgcgctgg gcttcctgtc ctgattcttc gccccggcac
    25021 gactgccggg gccatcgctc aacatgaggt ggtttatgaa aacaacagct ctgcgtcttt
    25081 atggtaaacg tgatttacgc ctggaaacct ttgaccttcc tgaaatgcag gaggatgaaa
    25141 tcctcgcgac ggtggtcact gacagcctgt gcctctcttc ctggaaagag gcaacctggg
    25201 tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac ccaatcatca tcggccacga
    25261 gttttgcggc gatattctgg ccgtgggtaa aaagtggcag cacaaattcc agccgggtca
    25321 gcgttatgtg attcaggcca acctgaaact ccctgaccgc ccggactgcc ccggctactc
    25381 cttcccgtgg gtgggcggcg aagccacgca tgtggttatt cccaacgagg tcatggaaca
    25441 agattgcctg ctggcatacg acggcgaaac ctattttgaa ggctcgctgg ttgaaccgct
    25501 ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt caggaaggta gttataacca
    25561 cacgatgggg attcgcccgc aagggcgcac gctgatcctc ggcggcaccg gaccaatggg
    25621 attgttggcg attgattatg cgctgcatgg accagttaac ccgtcgctgc tcgtcattac
    25681 cgataccgac aacgacaaat tgagttatgc gcgcaagcac tatccatcag aaccgcaaac
    25741 actgattcat tatctcaatg ccaccgatgc agcatttgat acgttaatgg cgctgagtgg
    25801 cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg tgactctcgc
    25861 ctcttccttg ctggcgacag atggttgcct gaatttcttc gccggaccgc aggataaaca
    25921 tttcagcgcg ccaattaatt tctacgatgt gcattatgca tttacccact acgtgggcac
    25981 gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa ctgattgaag agaaaaaagt
    26041 gcaggccgca aaagtggtaa cacatattct tgggctgaat gccgcgggcg aaaccacgct
    26101 tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat acctgccgct
    26161 gacgtcactc acgcagattc aggatcaagc actggcggcg attctggcgc gtcatcaggg
    26221 gatctggtcc ggtgaggcgg agcaatatct gctcactcat gcagaggcaa tttcccatga
    26281 ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg ccgcggcaac ttcgcgacct
    26341 gatgtgcggc tgattgctga agcggcccaa tgtgcacgag aaggataaaa aaacatcagc
    26401 gccttaaggg gcgctcaaat tggtggcaaa gtacacattg tttatgccgg atgcggaata
    26461 aagccttatt cggcctacat aatctcgaaa attcaatata ttgcagttat ctggtaggcc
    26521 tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc agtctcagcg cccggtgcaa
    26581 tacgtgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag catgccttcc
    26641 gtgatttctt actaaccgga gaagtaatgg aactgctttt attgagtaac tcgacgctgc
    26701 cgggtaaagc ctggctggaa catgcactgc cgctaattgc tgaacagttg cagggtcgcc
    26761 gctcagcggt gtttatccct ttcgctggcg taacgcagac ctgtgatgat tacacagcga
    26821 aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat agcgttgtcg
    26881 atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt cggggggcgg gaatactttc
    26941 cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga cgtggttaaa
    27001 cgtggtgcgc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc aaccattcgt
    27061 accaccaacg atatgccgat tgtcgatccg caaggtttcg atgcgctaaa tctgttcccg
    27121 ctgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg tgaaacccgt
    27181 gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat tggtctaccg
    27241 gaaggtaact ggatcacggt gagtaaaggt cacgctacgc tgggtggccc gaacaccact
    27301 tatgtgttta aggccggtga agaagcggtg ccgctggaag cgggtcaccg tttttaagtg
    27361 aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg cttgacgcgt
    27421 cttatcaggc ctacaggatt cacaatatta atactcgtcc cgtccttcat cttcatccgg
    27481 ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcagacgct gcatatctcc
    27541 cagtaagcct aaatgtagcg aactggtttc aatgctctgc acgttttgct gatgcaggcg
    27601 atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aagcgatgct tgctgcggcg
    27661 caaacgacga gcgctggtga catcgccaga gaagaacacc gacattgcca gctttaaatt
    27721 actgagcaat tgctcataga gcgcatccag ttccttcaac ccatcaagcg aaaatgcccg
    27781 ccgtgctgcc agcgatttat cagcaatttc gctgcccatg cgctcgacga tatcggaagc
    27841 ctgttcaagg ttgagcgaca tttcaataat ctccgcccag cgacgcgact cttcttctgc
    27901 cagttcctct tttggcattc gcgccagata cagcttaatg gcggtgtaga gaacgttgat
    27961 atcatctgcc agcttacgca gctctttctc ctgccgtggc tcgccgtgca tcactttatt
    28021 cagcccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc gcgcggcgtt
    28081 cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag gccgtagctg
    28141 ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac
    28201 aaatggcagc atgaccaggc aacgtacaag gttgtagaag acgtggaaat agatcaccag
    28261 ttccgctttt ggcaatgaca acttccccat tgtctctgcc agcaaatgga caaacggcag
    28321 gataatcagg ctacccacca gcttaaacag caaactaccc agcgcgacac ggcgggctgc
    28381 ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt tagcaccaat
    28441 caccagacag agcgccacgg ggaaggagat aatgcctgcg gcggtcagag tcgcggtcag
    28501 cagtacagca gcaaggctgg agtagctgat aatggcgaac atcgcgccaa tcagcgcatc
    28561 cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg cctgggtgat
    28621 cggcgttacg gcctgcacaa tcaactccag cgccagcaaa atcagcccaa gaccaatacc
    28681 gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat
    28741 aaaaataagt aacggtgaca gccaggataa gtcgaaggtg agaatacgcg ccattagcgc
    28801 cgtcccgaca tctgcaccca gcacaatcac cagagccggt gcgagggcta ccagatcctg
    28861 agcgacgaac gaggtcacca gcatggtggt ggcattactg ctctgtacca gtgcggtaac
    28921 gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa ggacagtacg
    28981 caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc cccagaccag
    29041 cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt ctccttatcg
    29101 ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga ctggcatctt
    29161 tactgtgcct tttgacattc gtttttccgg ctaagattat tccagcctgt ttccagtata
    29221 agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg ttgtaaggaa
    29281 aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca gccagcgctc
    29341 aacatcggag acgctcatac ctttacggcg ggcatagtct tcaacctgat cgcgctgaat
    29401 ttgcgccacc gcgtagtact tgctgtccgg gtggctgaaa taccaacccg aaaccgatgc
    29461 accaggccac atggcgaaag attctgtgag tttcatgcca gtatgttttt ccacttccag
    29521 cagctcccag atggtggctt tttccgtatg ttccgggcag gccgggtagc ccggtgccgg
    29581 acggatgccc tggtagtttt cgcggatcag ttcttcgttg ctgaggttct cgttcggcgc
    29641 atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg cttcggctaa
    29701 acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg cttcaaaggc
    29761 atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga tgtaatctgc
    29821 tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttagcga atcccgtttt
    29881 ttcggtctgt tgacgcagat ggtggctgac gttgatcaca tgggtgcgcg tttcgtcacg
    29941 gtagatttca atgtcatcgc ccacacggtt tgccgggaac aagcccacca cgccacgcgg
    30001 attcagcgtt ttctcggcgc ttaatttatc cagcatgtcg ttggcgtctt taaacagccg
    30061 ctgcgcctca acgcccacca cttcatcttc cagaatgcgc ggatacttcc cggccagcga
    30121 ccaggtcata aagaacggtg tccagtcgat gtaattacgc agagtttcga tgctggcttc
    30181 aacttcctgc acgccgagac ggtgcgccac cggcggcgtg taagcctgcc agtcaaaagc
    30241 gaagtcgtta tcgcgcgccg cttccagcgt gaccggtggt gtgcgcggtt tcttgcgccc
    30301 gtgctgaata cgtacggttt catactcctt gcgggtacga gcgacaaaat catcgcgctg
    30361 agtatcggaa agcagcgccg ccaccacacc aacggtgcgc gaggcattct gcacatacac
    30421 cgtcgggccg ctgtagttct gctcaatttt caccgccgtg tgcgcttttg aggtcgtcgc
    30481 gccaccgatc agtaacggaa tggtgaagcc ctgacgctcc atctctttcg ccacgttaac
    30541 catctcgtcc agcgacggcg tgataagccc cgaaagtcca atcagatcag cattcacttc
    30601 tttagcggta cggagaattt tttccgcagg caccataacg ccgagatcga caatttcgta
    30661 gttgttacat tgcagcacca caccaacgat atttttaccg atgtcgtgga cgtcgccctt
    30721 cacggtggcg atcaccatct tgccgttggt tttgccctgc tctttgctgg cttcaataaa
    30781 cggttcgagg taggccactg cctgtttcat gacgcgcgcc gatttgacca cctgtggcag
    30841 gaacattttc ccctcgccaa acagatcgcc gaccacgttc atgccgtcca tcaacgggcc
    30901 ttcaatcact tcaatcgggc gcgtagcctg ctggcgggct tcttcggtat cctgctcgat
    30961 aaactcagta atgcctttga ccagcgagta ttccagacgt ttattcactt cccacgagcg
    31021 ccactccgcc tgctgggcgt tagcggtgtc gtcggttttg ctgccgcgat atttctcggc
    31081 aagctccagt aaacgttcag tgccatcatc gcgacgatta agaatcacat cttccaccgc
    31141 gtcgcgcagt tcagcgggca ggtcgtcgta aatcgccagt tgcccggcgt tgacgatccc
    31201 catatccatg ccattgcgaa tagcgtagta gaggaacacc gcgtgaatgg cttcgcgcac
    31261 cggttcgttg ccacggaatg agaaagaaac gttagatacg ccgccggaaa tcagcgcgtg
    31321 cggcagttcg cgtttgatgt cttcgcaggc gccgataaag tcctgcgcat agttgttgtg
    31381 ctcttcaatg ccggtggcga ccgcgaagat gttcgggtcg aagatgatat cttctggcgg
    31441 gaagccaacc tcttcggtga ggattttgta cgcccgacgg caaatctcga ttttccgtgc
    31501 gcgggtatcg gcctgtccct gttcgtcaaa ggccattacc accaccgctg caccgtaacg
    31561 acgcaacagt ttcgcatgat ggataaaggc gtcgacgccc tctttcattg agatagagtt
    31621 aacaatgcct ttgccctgaa tacacttcag ccccttttcg atgacctccc attttgagga
    31681 gtcgatcata atcggcacgc gagcgatatc cggttcaccg gcaatcagat tgagaaaacg
    31741 caccatcgcc gcttcggcgt cgagcatccc ttcatccata ttgatatcga taatctgcgc
    31801 gccgttttcc acctgctggc gggccacatc cagtgcctcg ctgtatttct cttctttgat
    31861 caggcgcttg aacttagcgg aaccggtgac gttggtgcgt tcacccacgt tcacaaacag
    31921 gctatcttcg ccaatgttca gcggctccag gccggacaaa cggcaggcta cggggatttc
    31981 cggcagtttg cgcggcgcta atccttctac tgcacgactc atcgctgcaa tatgttgtgg
    32041 cgtggtgcca cagcagccgc cgacgatatt gagaaagccc gcttccgccc attcacgtat
    32101 ttgttttgcc atcgtgtcgg cgtcgagatc gtactcacca aaggcgttgg gtagcccggc
    32161 gttcgggtgc gcggtgacgt agcattccgc aatccgtgac agctcctgca cgtactggcg
    32221 cagttcatcg ggccccagcg cacagttcag gccaaaggtc agcgcttcgg cgtggcgcaa
    32281 tgagttgtaa aatgcttcgg tggtctgccc ggagagcgtg cgcccggagg cgtcggtgat
    32341 ggtgccagag atcataatcg gcagctcaac gcccagcgtt tcaaactccg tttttaccgc
    32401 aaataccgcc gctttggcgt taagggtgtc gaaaacggtt tcaatcagga tcagatccgc
    32461 accaccttcc accagcgctt tggtggactc tcgataagcc accaccagcc cgtcaaaagt
    32521 gatattacga aatgccggat cgttgacgtc cggagaaata gacgccgtgc ggttggttgg
    32581 gccgagaaca ccggcaacgt agcgcggttt ctccggcgtg cgcgcggtcc actcgtcagc
    32641 acaggcgcgc gccagtttcg ccgccgcaaa gttgatttcc gccgacaggg attccatctg
    32701 gtaatccgcc atcgcaatgg tcgtggagtt gaaggtgttg gtttcgatga tatccgcgcc
    32761 cgcttcaaag taggcgttgt ggatagcggc gatcacttcc ggtttactga gtaccagcag
    32821 gtcgttgttg cctttgaggt cgcatggcca gtcggcaaag cgttcaccac gaaaatcggc
    32881 ttcgttcagt cgatagctct ggatcatggt gcccataccg ccgtccagca ccagaatacg
    32941 ttcatttaac tgcgcacgca gttgttccac tttgctgctc acacttgctc ccgacacgct
    33001 caacccagat ttaataaaaa ttcaacaaac catactggca taaacgcatc tgtggtaaaa
    33061 gcgaccacca cgcaacatga gatttgttca acattaactc atcggatcag ttcagcaact
    33121 attgcattaa ctaacaataa aaatgaaaat gatttccacg atacagaaaa aggagactgt
    33181 catggtcgca cccattcccg cgaaacgcgg cagaaaaccc gccgttgcca ccgcaccagc
    33241 gactggacag gttcagtctt taacgcgtgg cctgaaatta ctggagtgga ttgccgaatc
    33301 caatggcagt gtggcactca cggaactggc gcaacaagcc gggttaccca attccacgac
    33361 ccaccgcctg ctaaccacga tgcaacagca gggtttcgtg cgtcaggttg gcgaactggg
    33421 acactgggca atcggcgcac atgcctttat ggtcggcagc agctttctcc agagccgtaa
    33481 tttgttagcg attgttcacc ctatcctgcg caatctaatg gaagagtctg gcgaaacggt
    33541 caatatggcg gtgcttgatc aaagcgatca cgaagcgatt attatcgatc aggtacagtg
    33601 tactcacctg atgcgtatgt ccgcacctat cggcggtaaa ttgccgatgc acgcttccgg
    33661 tgcaggtaaa gcctttttag cccaactgag cgaagaacag gtgacgaagc tgctgcaccg
    33721 caaagggtta catgcctata cccacgcaac gctggtgtct cctgtgcatt taaaagaaga
    33781 tctcgcccaa acgcgcaaac ggggctattc atttgacgat gaggaacatg cactggggct
    33841 acgttgcctt gcagcgtgta ttttcgatga gcaccgtgaa ccgtttgccg caatttctat
    33901 ttccggaccg atttcacgta ttaccgatga ccgcgtgacc gagtttggcg cgatggtgat
    33961 taaagcggcg aaggaagtga cgctggcgta cggtggaatg cgctgacttt ttctggcggg
    34021 cagaggcaat attctgccca tcatacctga gtggcaatag aataagggtg tctgttaatc
    34081 gcattgacgc caaaataact taatgtcata cacttcacac acttttatta actcagcatt
    34141 atttttaaac atcaaaccac ttaattatta acaaaatcgc aagatctcat tccaccgaaa
    34201 cttttacggc tgcattatga
//
LOCUS       DAZOXR010000024        33999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZOXR010000024 DAZOXR010000000
VERSION     DAZOXR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.024"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            12..514
                     /locus_tag="ACHUNN_000989"
                     /pseudo
     CDS             12..514
                     /locus_tag="ACHUNN_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUNN_000990"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUNN_000990"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0575436.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUNN_000991"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUNN_000991"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0575437.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUNN_000992"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUNN_000992"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0575438.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUNN_000993"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUNN_000993"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0575439.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUNN_000994"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUNN_000994"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0575440.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUNN_000995"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUNN_000995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0575441.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUNN_000996"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUNN_000996"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0575442.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUNN_000997"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUNN_000997"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0575443.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUNN_000998"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUNN_000998"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0575444.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUNN_000999"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUNN_000999"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0575445.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUNN_001000"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUNN_001000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0575446.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUNN_001001"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUNN_001001"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0575447.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUNN_001002"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUNN_001002"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0575448.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHUNN_001003"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHUNN_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0575449.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="ACHUNN_001004"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="ACHUNN_001004"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0575450.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="ACHUNN_001005"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="ACHUNN_001005"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0575451.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="ACHUNN_001006"
     CDS             complement(20046..21878)
                     /locus_tag="ACHUNN_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0575452.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="ACHUNN_001007"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="ACHUNN_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="ACHUNN_001008"
     CDS             complement(22624..23118)
                     /locus_tag="ACHUNN_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0575453.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /gene="yfbV"
                     /locus_tag="ACHUNN_001009"
     CDS             complement(23201..23656)
                     /gene="yfbV"
                     /locus_tag="ACHUNN_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0575454.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="ACHUNN_001010"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="ACHUNN_001010"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0575455.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="ACHUNN_001011"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="ACHUNN_001011"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0575456.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="ACHUNN_001012"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="ACHUNN_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0575457.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="ACHUNN_001013"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="ACHUNN_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0575458.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="ACHUNN_001014"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="ACHUNN_001014"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0575459.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="ACHUNN_001015"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="ACHUNN_001015"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0575460.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="ACHUNN_001016"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="ACHUNN_001016"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0575461.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="ACHUNN_001017"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="ACHUNN_001017"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0575462.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="ACHUNN_001018"
     CDS             32232..33125
                     /locus_tag="ACHUNN_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0575463.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="ACHUNN_001019"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="ACHUNN_001019"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0575464.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8481 a         9279 c         8562 g         7677 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccaag cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctagtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAZOXR010000025        32837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZOXR010000025 DAZOXR010000000
VERSION     DAZOXR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.025"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUNN_001020"
     CDS             267..1925
                     /locus_tag="ACHUNN_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0575465.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUNN_001021"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUNN_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0575466.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUNN_001022"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUNN_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0575467.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUNN_001023"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUNN_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0575468.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUNN_001024"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUNN_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0575469.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUNN_001025"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUNN_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0575470.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUNN_001026"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUNN_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0575471.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUNN_001027"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUNN_001027"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0575472.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHUNN_001028"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHUNN_001028"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUNN_001029"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUNN_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0575473.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUNN_001030"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUNN_001030"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0575474.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUNN_001031"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUNN_001031"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0575475.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUNN_001032"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUNN_001032"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0575476.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUNN_001033"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUNN_001033"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0575477.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUNN_001034"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUNN_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0575478.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUNN_001035"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUNN_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0575479.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUNN_001036"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUNN_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0575480.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUNN_001037"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUNN_001037"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0575481.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHUNN_001038"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHUNN_001038"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0575482.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUNN_001039"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUNN_001039"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR0575483.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUNN_001040"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUNN_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0575484.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUNN_001041"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUNN_001041"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR0575485.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUNN_001042"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUNN_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0575486.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHUNN_001043"
     CDS             29803..30951
                     /locus_tag="ACHUNN_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575487.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHUNN_001044"
                     /pseudo
     CDS             31161..32591
                     /locus_tag="ACHUNN_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(32592..>32837)
                     /locus_tag="ACHUNN_001045"
     CDS             complement(32592..>32837)
                     /locus_tag="ACHUNN_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0575488.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8030 c         8813 g         7937 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcac
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtacgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcttc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggct tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccctaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatc
//
LOCUS       DAZOXR010000026        32533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZOXR010000026 DAZOXR010000000
VERSION     DAZOXR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.026"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHUNN_001046"
     CDS             <1..94
                     /locus_tag="ACHUNN_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0575489.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="ACHUNN_001047"
     CDS             complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="ACHUNN_001047"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0575490.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="ACHUNN_001048"
     CDS             complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="ACHUNN_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0575491.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2848..4476
                     /gene="glpA"
                     /locus_tag="ACHUNN_001049"
     CDS             2848..4476
                     /gene="glpA"
                     /locus_tag="ACHUNN_001049"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0575492.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4466..5725
                     /gene="glpB"
                     /locus_tag="ACHUNN_001050"
     CDS             4466..5725
                     /gene="glpB"
                     /locus_tag="ACHUNN_001050"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0575493.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5722..6912
                     /gene="glpC"
                     /locus_tag="ACHUNN_001051"
     CDS             5722..6912
                     /gene="glpC"
                     /locus_tag="ACHUNN_001051"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0575494.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7105..8007
                     /locus_tag="ACHUNN_001052"
     CDS             7105..8007
                     /locus_tag="ACHUNN_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0575495.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="ACHUNN_001053"
     CDS             complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="ACHUNN_001053"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR0575496.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8869..10158)
                     /locus_tag="ACHUNN_001054"
     CDS             complement(8869..10158)
                     /locus_tag="ACHUNN_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575497.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="ACHUNN_001055"
                     /pseudo
     CDS             complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="ACHUNN_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11434..12216)
                     /locus_tag="ACHUNN_001056"
     CDS             complement(11434..12216)
                     /locus_tag="ACHUNN_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR0575498.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12436..13638)
                     /locus_tag="ACHUNN_001057"
     CDS             complement(12436..13638)
                     /locus_tag="ACHUNN_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0575499.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13738..14280)
                     /locus_tag="ACHUNN_001058"
     CDS             complement(13738..14280)
                     /locus_tag="ACHUNN_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0575500.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14559..14984
                     /gene="nudI"
                     /locus_tag="ACHUNN_001059"
     CDS             14559..14984
                     /gene="nudI"
                     /locus_tag="ACHUNN_001059"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0575501.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15023..15625)
                     /gene="ais"
                     /locus_tag="ACHUNN_001060"
     CDS             complement(15023..15625)
                     /gene="ais"
                     /locus_tag="ACHUNN_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0575502.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15933..17072
                     /gene="arnB"
                     /locus_tag="ACHUNN_001061"
     CDS             15933..17072
                     /gene="arnB"
                     /locus_tag="ACHUNN_001061"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0575503.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17076..18044
                     /gene="arnC"
                     /locus_tag="ACHUNN_001062"
     CDS             17076..18044
                     /gene="arnC"
                     /locus_tag="ACHUNN_001062"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0575504.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18044..20026
                     /gene="arnA"
                     /locus_tag="ACHUNN_001063"
     CDS             18044..20026
                     /gene="arnA"
                     /locus_tag="ACHUNN_001063"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR0575505.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20023..20913
                     /gene="arnD"
                     /locus_tag="ACHUNN_001064"
     CDS             20023..20913
                     /gene="arnD"
                     /locus_tag="ACHUNN_001064"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0575506.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20913..22565
                     /gene="arnT"
                     /locus_tag="ACHUNN_001065"
     CDS             20913..22565
                     /gene="arnT"
                     /locus_tag="ACHUNN_001065"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0575507.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22562..22897
                     /gene="arnE"
                     /locus_tag="ACHUNN_001066"
     CDS             22562..22897
                     /gene="arnE"
                     /locus_tag="ACHUNN_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0575508.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22897..23283
                     /gene="arnF"
                     /locus_tag="ACHUNN_001067"
     CDS             22897..23283
                     /gene="arnF"
                     /locus_tag="ACHUNN_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0575509.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="ACHUNN_001068"
     CDS             complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="ACHUNN_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0575510.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23587..25008)
                     /gene="menE"
                     /locus_tag="ACHUNN_001069"
     CDS             complement(23587..25008)
                     /gene="menE"
                     /locus_tag="ACHUNN_001069"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0575511.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25005..25967)
                     /gene="menC"
                     /locus_tag="ACHUNN_001070"
     CDS             complement(25005..25967)
                     /gene="menC"
                     /locus_tag="ACHUNN_001070"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0575512.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAEMADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25967..26824)
                     /gene="menB"
                     /locus_tag="ACHUNN_001071"
     CDS             complement(25967..26824)
                     /gene="menB"
                     /locus_tag="ACHUNN_001071"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0575513.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26839..27597)
                     /gene="menH"
                     /locus_tag="ACHUNN_001072"
     CDS             complement(26839..27597)
                     /gene="menH"
                     /locus_tag="ACHUNN_001072"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0575514.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27594..29264)
                     /gene="menD"
                     /locus_tag="ACHUNN_001073"
     CDS             complement(27594..29264)
                     /gene="menD"
                     /locus_tag="ACHUNN_001073"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0575515.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29353..30648)
                     /gene="menF"
                     /locus_tag="ACHUNN_001074"
     CDS             complement(29353..30648)
                     /gene="menF"
                     /locus_tag="ACHUNN_001074"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0575516.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="ACHUNN_001075"
     CDS             complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="ACHUNN_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0575517.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31087..31530)
                     /locus_tag="ACHUNN_001076"
     CDS             complement(31087..31530)
                     /locus_tag="ACHUNN_001076"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0575518.1"
                     /translation="MHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDNRH
                     ILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDTCTHHWP
                     DKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            31613..32530
                     /gene="rbn"
                     /locus_tag="ACHUNN_001077"
     CDS             31613..32530
                     /gene="rbn"
                     /locus_tag="ACHUNN_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0575519.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7896 a         8806 c         8138 g         7693 t
ORIGIN      
        1 ttcgttgttg gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg
       61 catggaacaa ttagtggcga ttggaaaaat ttgatatgag acaggatggc gcatgaacgc
      121 catcctgcag tagcattact ctttattaag gaattttact gccttatcag ggaaatcagt
      181 aaacagccca tttacacccg ctttgttata cagagcatca tataactgat tcacatcagt
      241 agtgtattca ggcagtttat ccgaccgcac ggtataagga tgcactacca gtttattctg
      301 ctgagcatct tgcaccatgc cagtgagttt gatattaccc ggctgcgatg tttcctcaat
      361 caacatatgg taatccggcc cgataccgtc ggcatattcc gcgacttgct tcatggcacc
      421 tggcttaaac atccagtcat agctgtaatt aacccagctt ccgtccggct gtttctgctg
      481 cgtttcattc cagtcggtat aggcaatcag ctgtaccaga ttgagatcca tgcccatttt
      541 aggttccagc tcattcttaa tacgcttcag ctcatcagca tcaaaacatt gcaaataaac
      601 tttatcgtcc ttaccggtgt aaccatattt cttcagcact tccagcgttt ttgccgcaat
      661 atccttccct tcctgatgat ggaaccacgg cgctttgatt tctggataga taccgatatt
      721 tttcccggta gagtgattta acccctgaac aaattcaatc tcttcttcaa aggtgtgccc
      781 ccggaagtcg gacttaccca ttgggaaacg ccccggataa gtctgtactt ttttaccgtt
      841 ttcaatatcg aaaccttcgg taaatttcag cgacttaatt tcatccagcg tgaaatctat
      901 cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa cgatcggcaa catcagtaac
      961 acgatcgaga taatggtcat gcagaacaac cagatggtcg tctttggtca tcaccaaatc
     1021 ctgttccaga taatccgctc cctgcgcata cgccatcgct tttgctggca gcgtatgctc
     1081 cggcaaatat ccactggcac cgcgatgggc gatgactatt ttttcgttgc tgtccgccgc
     1141 cattgcactg cttcccatga ctatagtgct catcatgatc gccatgctaa ggtttttcag
     1201 cttcaatttc atgccattag cctccgttgc gtttttgcag taattgttca tggcgacgtt
     1261 tttcgccaat catcacaaca atcaacaaga taaccgccag aatgctgccg ccaatcatta
     1321 ccataaagcc gccatcccag ccgaagaagt ccacggtgta gccaacaatc gcgctcgccg
     1381 ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc cgctgccgta cctgccgctt
     1441 ttttcggtgc cagttccagc gcatgcagac cgatcagcat cacaggaccg tagatcagga
     1501 agccgataac aatcatacaa atcatatcga cggttgggtt acctgccggg ttcatccagt
     1561 aaacgatagt cgcgatggtc accagtgtca taaagaaaac gccggttgcc ccacggttgc
     1621 cacggaagac tttatccgac atccagccgc acagcagagt gcccggaata cctgcatatt
     1681 cataaaggaa gtaggcccag gaggatttat ccagcgcgaa atgcttaacc tctttcagat
     1741 aagtcggtga ccagtcgagg atgccgtaac gcagcagata aacgaacacg ttggcgatgg
     1801 cgatatacca cagcagtttg ttcggcagta cgtactgcat gaagatttgc ttcgccgtca
     1861 gctcctgttc cgctttttcg ttatagtcgt ccggataatc atttttgtac tcttcgatcg
     1921 gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc aaaggcgaat aatgccacca
     1981 gaatggcgca gaaagcaggc atatagagcg ccgcatgcca gtcattgaac caggccatcc
     2041 ccagcaggaa cagcagcggc ggaataccac caccgacgtt gtgcgcacag ttccacactg
     2101 acacaatgcc gccacgttct ttctgcgacc accagtgcac catagtacga ccacacggcg
     2161 gccaccccat cccctggaac caaccgcaga ggaacaacag tacaaacatc accgcaatgc
     2221 tcgacgtcgc ccatggcaca aagcccataa acaacatcac tgccgccgcc agaatcaaac
     2281 ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac cgaacccatg atgaatttcg
     2341 aaaatccata agcaatcgag atccccgaaa gggcaaaacc taaatcaccg cgtgagaatc
     2401 cctgctcaac cagataaggc atagcaagcg caaagttctt acgaaccaaa tagtaagccg
     2461 catagccaaa gaatatcccc aggaaaattt gccagcgcaa tcgacgataa gtcggatcga
     2521 tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt aaaaatactc aacattgata
     2581 gcctccgtgg cccgtggtct tatttatgat taacagcctg attcagtgag agaacctgcc
     2641 gtttcttgag ttgccgcgat gttaagaaaa cattcataaa ttaaatgtga attgccgcac
     2701 acattattaa ataagattta caaaatgttc aaaatgacgc atgaaatcac gcttcacttt
     2761 cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg tttttactat ggctaaatgg
     2821 taaaaaacga acttcagagg gataacaatg aaaactcgcg actcgcaatc aagtgacgtg
     2881 attatcattg gcggcggcgc aacgggagcc gggattgccc gcgactgtgc cctgcgcggg
     2941 ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg gtgccaccgg gcgtaaccac
     3001 ggcctgctac acagcggtgc gcgctatgcg gtaaccgatg cggaatcggc ccgcgaatgc
     3061 attagtgaaa accagatcct gaaacgcatt gcacgtcact gcgttgaacc aaccaacggc
     3121 ctgtttatta ccctgccgga agatgacctc tccttccagg ccacttttat tcgcgcctgc
     3181 gaagaagcag ggatcagcgc agaagctata gacccgcagc aagcgcgcat tatcgaacct
     3241 gccgttaacc cggcattgat tggcgcggtg aaagttccgg atggcaccgt tgatccattt
     3301 cgtctgaccg cagcaaacat gctggatgcc aaagaacacg gtgccgttat ccttaccgct
     3361 catgaagtca cgggactgat tcgtgaaggc gcgacggtgt gcggtgttcg tgtacgtaac
     3421 catctcaccg gcgaaactca ggcccttcat gcgcctgtcg tggttaatgc cgccgggatc
     3481 tgggggcaac acattgccga atatgccgat ctgcgcattc gcatgttccc ggcgaaagga
     3541 tcgctgctga tcatggatca ccgcattaac cagcatgtga tcaaccgctg ccgtaaacct
     3601 tccgacgccg atattctggt gcctggcgat accatttcgc tgattggcac cacctcttta
     3661 cgtattgatt acaacgagat tgacgataat cgagtgacgg cagaagaggt tgatattctg
     3721 ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa cgcgcatttt gcgggcctat
     3781 tctggcgtgc gcccgctggt tgccagcgat gacgacccga gcggacgtaa cgtcagccgt
     3841 ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg acggatttat caccatcacc
     3901 ggtggcaaac tgatgaccta tcggctgatg gctgaatggg ctaccgacgc cgtgtgccgc
     3961 aaactgggca acacgcgccc ctgtacgacc gccgatctgg cacttcctgg ttcacaggaa
     4021 cccgctgaag ttaccttgcg taaagtcatc tccctgcctg ccccgctgcg cggttctgcg
     4081 gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg aaggtcgtct gcaccgtagc
     4141 ctggtatgtg agtgcgaagc ggtaacagcg ggtgaagtgc agtacgcggt agaaaattta
     4201 aacgttaata gcctgctgga tttacgccgt cgtacccgtg tggggatggg tacctgccag
     4261 ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac gttttaacgt cacgacgtcc
     4321 gcgcaatcta tcgagcaact ttccaccttc cttaacgaac gctggaaagg cgtgcaaccc
     4381 atcgcctggg gagatgcact gcgcgaaagc gaatttaccc gctgggttta tcagggactg
     4441 tgtggtctgg agaaggagca gaaagatgcg ctttgatact gtcattatgg gcggcggcct
     4501 cgccggatta ctctgtggcc tgcaactgca aaaacacggc ctgcgctgtg ccattgtcac
     4561 gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg gatttgctga gccatctgcc
     4621 tgatggtcaa ccggtgacag acattcacag tggactggaa tctttgcgtc agcaggcacc
     4681 agcccatcct tacagtcttc tcgaaccaca acgcgtgctc gatctcgctt gccaggcgca
     4741 ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc gtggaactgg cccatcagcg
     4801 cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt tcgccagaag ttcccgtctg
     4861 gccgctaccc gcgaagaaga tatgtgtagt ggggataagc gggctgatgg atttccaggc
     4921 gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg gttgaaaccg cagaaataga
     4981 gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa tttcgcgcgg tgaatatcgc
     5041 ccgtttcctt gataatgaag aaaactggcc gctgttactt gatgcgctta ttcctgtcgc
     5101 caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc ctggccgatg acaaactgtg
     5161 gcgttggttg aatgaaaaac taccttgttc actgatgctt ttgccaacgc tgccgccttc
     5221 cgtgctgggc attcgtctgc aaaaccagtt acagcgccag tttgtgcgcc agggtggcgt
     5281 gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa aatggcgtag tgaatgaaat
     5341 ctggacccgc aatcacgccg atattccgct acgtccacgt ttcgcggttc tcgccagcgg
     5401 cagtttcttt agtggcggac tggtagcgga acgtaacggc attcgcgagc cgattctcgg
     5461 ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat aagggcgatt tttttgcgcc
     5521 gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg ctacgcccgt cgcaggcagg
     5581 gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg ggcggatttg atcccatcgc
     5641 ccaaggatgc ggcggcggtg tttgtgccgt cagtgcttta catgccgctc aacagattgc
     5701 ccaacgcgca ggaggccaac aatgaacgac accagcttcg aaaactgcat taagtgcacc
     5761 gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg gttatccagg gccaaaacaa
     5821 gccgggccgg atggcgagcg tctgcgtttg aaagatggcg cactgtatga cgaggcgctg
     5881 aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc cgtccgatgt gaagattggc
     5941 gatattatcc agcgcgcgcg ggcaaaatat gacaccacgc gcccgtcgct gcgtaatttt
     6001 gtgttgagtc ataccgacct gatgggtagc gtttccacgc cgttcgcacc aatcgtcaac
     6061 accgctacct cgctgaaacc ggtgcggcag ctgcttgatg cggcgttaaa aatcgatcat
     6121 cgtcgcacgc taccgaaata ctccttcggc acgttccgtc gctggtatcg cagcatagcg
     6181 gctcagcaag cacaatataa agaccaggtc gctttctttc acggctgctt cgttaactac
     6241 aaccatccgc agttaggtaa agatttaatt aaagtgctca acgcaatggg tatcggtgta
     6301 caactgctca gcaaagaaaa atgctgcggc gtaccgctaa tcgccaacgg ctttaccgat
     6361 aaagcacgca aacaggcaat tacgaatgta gagtcgatcc gcgaagctgt gggagtaaaa
     6421 ggcattccgg tgattgccac ctcctcaacc tgtacatttg ccctgcgcga cgaatacccg
     6481 gaagtgctga atgtcgacaa caaaggcttg cgcgatcata tcgaactggc aacccgctgg
     6541 ctgtggcgca agctggacga aggcaaaacg ttaccgctga aaccgctgcc gctgaaagtg
     6601 gtttatcaca ctccgtgcca tatggaaaaa atgggctgga cgctctacac cctggagctg
     6661 ttgcgtaaaa tcccggggct tgagttaacg gtgctggatt cccagtgctg cggtattgcg
     6721 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg cgcaccactg
     6781 ttccgccaga tagaagaaag cggcgcagat ctggtggtca ccgactgcga aacctgtaaa
     6841 tggcagattg agatgtccac aagtcttcgc tgcgaacatc cgattacgct actggcccag
     6901 gcgctggctt aaactccttt ctgatgcccg gtaagcatgt ggttaccggg catttttgcg
     6961 tacacgattc cgtgcccaat gtatgcgttg caacgcagtg aaaattcctc tgaaaacgtc
     7021 tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat aaccgtaatg tcgcgtcatc
     7081 ataaatatca ggtgacggac aaccatgacc gaatcaacaa cctcctcccc gcatgatgcg
     7141 gtatttaaaa cctttatgtt cacacccgaa accgcacggg attttctcga aatacatttg
     7201 ccagaaccac tgcgcaagct ttgcaacctg caaaccttac gcctggaacc cactagtttt
     7261 attgaaaaaa gtttacgcgc ttactactcg gatgttttgt ggtccgtgga aaccagcgac
     7321 ggtgacggct atatctactg cgtgattgaa catcaaagct ctgcagaaaa gaatatggct
     7381 tttcggctaa tgcgctatgc cactgccgcc atgcagcgtc acctggataa aggctatgac
     7441 agagttccgc tggtggtgcc attgctgttt tatcatggcg aaacatcgcc ctacccgtac
     7501 tcacttaact ggctggatga gtttgacgat ccgcaacttg cccggcagtt gtacaccgaa
     7561 gcttttccgt tggtggatat caccatcgta cctgacgatg agatcatgca acatcggcgt
     7621 atagctctgc ttgaactgat tcaaaagcat attcgcgacc gcgatttaat cggtatggtc
     7681 gacaggatca ccacgctttt ggttaaaggc ttcactaatg acagccagct acaaacactg
     7741 tttaattatc tgctgcaatg cggcgatacc tcccgtttca cccgttttat tgaggagatt
     7801 tccgaacgtt caccactaca aaaggagaga ttgatgacta ttgctgagcg gctacggcag
     7861 gaaggacacc aaattggctg gcagaaaggt atgcatgaac aagccattaa aattgctttg
     7921 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
     7981 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
     8041 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
     8101 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
     8161 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
     8221 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
     8281 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
     8341 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
     8401 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
     8461 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggagga tagcgcgtgg
     8521 cagacgccac ctgacgagcc tgatcggcag tatcgaccat cgggatcaat agagtttgcg
     8581 cgccaatatc cagaacttgt ttaatcagcg atttactgcc ttccaccgga cggatcacgg
     8641 gttggctggc atagggcgct accgcctgta gctggtgata aagatcctga atggtgtttg
     8701 gcgcgtgctc cccgtcaatc agcaaccagt cataaccgga agtagcggca atttctgcca
     8761 tataagaagt agtcgagctt aaccacagac caatttgcac ttcgcccttg cgtaaacgtt
     8821 ctttaaaggg attggttaat aatgcgttca tcgtgttcct taacctattt aatgatgtgc
     8881 cacgtcggtc tgcgcaacag tacgatttac acgcagagtg aaaataatca gcgaaccgat
     8941 gaccgccacc gctgccagtg tcagtaatcc tgccgcatcg ctggcaaata acgtttctgc
     9001 tttcacgcgc aggatcgggg caataaaccc gcccactgca ccaaacaggt ttacaaaacc
     9061 aatccccgca gccagcgccg taccggaaag cagttgtgtc ggcatcgtcc agaacaccgg
     9121 ctgcacggca ataaacccga tagctgcaac acacagcgct acgatcgcca ttactggaga
     9181 aagcagcccg gacagaccaa taccaatgcc cgccgccagt aatgtcagcg ctgcgacatt
     9241 acgccgttcg ccggttttat cggaatagcg cggaataagc caggtcccaa acaaggccgc
     9301 aacccacgga atggcggtga ccaccgacgc tgtaaagccc acttttgttc ccagcaatgc
     9361 cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc gccacctgaa tggtcaggta
     9421 gataatcgct agttgccaga ctcggccatt acgcagcgca tcgctcagcc gagaagtcac
     9481 tttctgttgt tcttcacttg ccagttgatt gataagcaac gttttttctt gtttactcag
     9541 aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat gtgaataccc cagcaccgac
     9601 tgccaacaat ccttcaatca caaacatcca gaaccagccg ggatgcccca taaatccatg
     9661 catctccaac agcgcgccgg aaagcggtga tcccagtgtt aacgccagcg gtgcgcccat
     9721 atagaacagc cccataatgc tggcgcgatt acgctgcgga aaccattgcg aggtgagata
     9781 aatcatacca gggaaaaatc cggcctccgc agcaccaagc agagtgcgaa ctatcagaaa
     9841 tttcgcttca gtatcggccc atgccatggc tgccgaaaga aatccccaca gcagtgttgt
     9901 cgtaccaatc caggttctgg cccccagttt gcgcatcaaa agattcgccg gaacacccag
     9961 aaacgcatat accacaaaga aaatgcctgc tcccagcgca taagcttcat tactcaaccc
    10021 ggtatcaatc tggtaggtct gtttggcaaa accgatattc gaacggtcga gaaacgccag
    10081 cacatacagc gccaacataa acggaattaa acgcacacgg tttttcttca ccacggcgtc
    10141 aagcaaagcg gtgctcataa aaagctcctt agaatatgtg ggcgttaccg ttgtcacagc
    10201 aacgcccgga gtgattagtg gctgtagggg cgtttcaaat tgcagtcacg attgagttcg
    10261 acgccaaaac cgggtttatc aagcactgat ttatgaatac gaccattcac cggaaccggc
    10321 tcattgagca gaatcgggtc aaactgcgga cgcatcgttg aacaatccgg gctggtcatc
    10381 aggaattcgc tgaatggcgt attggtgaag gtgatcaccg catggtgaga gtaaacagac
    10441 gaaccgtgcg gcaccaccag ttgccccctg gatttggcga ttgcggcaat ttccaccagc
    10501 gtggttaaac caccgcacca gccaacatcc ggctgcataa tgtcgatacc ggtttctgaa
    10561 agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc tggtgaccat cattccgact
    10621 ggcgcgttgc gtttcagttc gcgataacct tcatactgct gtggcggcag gcactcttcg
    10681 atccatttca ggttataggg cgcgcaagcg tgggccagtt tggtggcata gttcacgtcc
    10741 tgactcatcc agcagtcgag cattaaccag aaatcctcac cgcatttttc acgcatatcc
    10801 gcgaccataa cggcatcttt gcggatcccc gcatcgccat catgtggccc ccagtgcgtc
    10861 ggcattttgc caccgataaa gcccatctct tttgccagat ccggacgcgc gcctgtggcg
    10921 tagaactgaa tctcatcacg aactgcgccg cctaaaagtt tataaaccgg aagcccgacc
    10981 actttgccga acagatccca cagagccaga tcgacacagg aaatcgtgtt catcaccagg
    11041 ccaccagagc cggagtaata cagggtggca ttgagcattt gatcatggat cagtttgata
    11101 tcactgacac atttaccctc aatgaaacgg ttaagatgtt tttcgacaat aaaacagccc
    11161 atttcaccgg ctgtcgaaac agcgaatccg gtctggccgt tttctgcttc gacttcaaca
    11221 atcaacgtgc caagaacgtt aatgccaaat gactggcgtg actgctcgta atcgcggtat
    11281 ttactcatcg gggtggcaat atgatcgtca atccagtgat tcgccccctg gtcgtgataa
    11341 tcaccgcgcc agcacctttt tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt
    11401 tgatttttgg tagggtcatg atgttctcca ttgttatgag gcctgtaagt caaagggact
    11461 tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc ccgccagata
    11521 atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg cagcggtaag
    11581 ctcgttattg gcgttaaaca ccggcgcagc gacacaacgg acatcggggt aatcttcgcc
    11641 gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg
    11701 atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa tactttgctg
    11761 taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat aaagcgaaag
    11821 gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt ttaaaatgta
    11881 ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg tatccatcag
    11941 ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg catgaccaga
    12001 aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc taagaaaacg
    12061 ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc
    12121 aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa tatcaatagc
    12181 ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc ttagatactg
    12241 gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg
    12301 tccagcggac tggagaaatt acgcacaaat ttgtgaatca gtgcacggat tttttcttat
    12361 tgagtacaac aggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    12421 cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa tccagccatg
    12481 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    12541 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    12601 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    12661 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    12721 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    12781 agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg
    12841 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    12901 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    12961 tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac
    13021 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    13081 ggtagttaaa cgcagacaaa ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg
    13141 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    13201 caggcaacga ttaagccgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    13261 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    13321 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc
    13381 ttttgctgtc gcagcggcga gtgcacttaa atcatcactg gtcggtccca gcccgccgtt
    13441 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    13501 gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc
    13561 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggataa
    13621 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    13681 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    13741 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    13801 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggat gcataatggt
    13861 gtaacgcgcg ccagcattcg cttcctcata acttttaata ccgctggaga gtgaatccgg
    13921 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    13981 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    14041 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgccagcgac
    14101 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg tcgattcagt
    14161 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga tgctcattgc
    14221 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    14281 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    14341 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    14401 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    14461 aagtgcagtt atcatcgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    14521 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    14581 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    14641 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    14701 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    14761 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    14821 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    14881 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    14941 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    15001 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    15061 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    15121 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    15181 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    15241 gtttatctac cgtcagtttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    15301 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    15361 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    15421 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    15481 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    15541 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    15601 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    15661 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    15721 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    15781 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    15841 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    15901 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    15961 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    16021 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    16081 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    16141 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    16201 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    16261 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    16321 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    16381 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    16441 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    16501 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    16561 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    16621 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    16681 tagcaaaatt agagcacctc aacacccatc ggcgcgaaat tgcccagcaa tatcagcaag
    16741 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    16801 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    16861 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    16921 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    16981 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    17041 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    17101 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    17161 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    17221 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    17281 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    17341 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    17401 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    17461 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac aggcttattc
    17521 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    17581 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    17641 atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt gagtttggtg
    17701 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    17761 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    17821 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    17881 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    17941 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    18001 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    18061 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    18121 tagcgccatt tttacccata ccgataatcc cgctgaaaaa gccttttatg gttcggtggc
    18181 tcgtctggcg gcggaaagag gcattccagt ttatgcgccg gataacgtta atcatccgct
    18241 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
    18301 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    18361 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    18421 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    18481 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    18541 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    18601 ggaaattgcc cagcgcgaaa acgaagccac ctgttttggt cacagaacgc cagatgacag
    18661 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    18721 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    18781 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    18841 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    18901 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    18961 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    19021 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    19081 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
    19141 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    19201 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    19261 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    19321 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    19381 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    19441 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    19501 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    19561 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    19621 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    19681 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    19741 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    19801 aaaacatccg ctgcgccatc acttcccgcc gtttgcgggc tttcgtgttg tcgaaagtag
    19861 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    19921 ccgccgctgc ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    19981 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
    20041 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc
    20101 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    20161 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    20221 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    20281 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    20341 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    20401 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    20461 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    20521 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    20581 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    20641 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    20701 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    20761 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    20821 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    20881 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    20941 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    21001 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    21061 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    21121 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    21181 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    21241 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    21301 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    21361 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    21421 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    21481 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    21541 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    21601 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    21661 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    21721 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    21781 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    21841 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    21901 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    21961 tcgcttttgg cttcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    22021 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    22081 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
    22141 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    22201 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    22261 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    22321 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    22381 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    22441 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    22501 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    22561 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    22621 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    22681 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    22741 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    22801 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    22861 tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    22921 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    22981 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    23041 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    23101 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    23161 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    23221 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    23281 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    23341 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    23401 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    23461 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    23521 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    23581 tacagattaa cagataaacc tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa
    23641 actaatgatt tcgtaatcct ggcgttgcac ccactctttt aacgcctgac gtgaaatttt
    23701 aataccgccg tttttcagct ccggcggtaa agttagccag cgcaccggtt gttgaaaacg
    23761 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    23821 cgctaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    23881 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
    23941 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    24001 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    24061 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    24121 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    24181 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    24241 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    24301 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    24361 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    24421 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    24481 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    24541 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    24601 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    24661 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    24721 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    24781 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    24841 catcacgccg ctgccctcaa ccacaccctg cgccgcaaag cccgtagcta actcatcgac
    24901 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    24961 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    25021 agtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    25081 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    25141 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    25201 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    25261 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    25321 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    25381 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    25441 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    25501 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    25561 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    25621 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    25681 tctgccatct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    25741 tcgccatcgc ctgccagcca gttatttacc caagcaagca gcacaatttg cgcctcttcc
    25801 caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    25861 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc
    25921 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    25981 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    26041 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cccgcctgac
    26101 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    26161 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    26221 tgacaaggcc catatccagc gcctgttttg catcgtactg acggcacagg aaccagattt
    26281 cacgcgcttt tttctgtccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    26341 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    26401 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    26461 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    26521 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    26581 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttgtcgtca taacgcgcat
    26641 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    26701 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa
    26761 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    26821 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga taacgccagc
    26881 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    26941 ttccgccgcc aaggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    27001 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    27061 catggcggca agggttgcgc cattattgtt actgcgcagc gccaccagct cccggcgttg
    27121 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    27181 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    27241 ttcagcattt tgcaacccag gatgcccgcc ttcgacaaca accccacaaa gccccggcat
    27301 cccctggcaa gccgccatca tcgccacccg tcccccaagc gagtacccca ccaaccagaa
    27361 gttaaggatg ttgtaactaa ccagggtttt acgcagtaag tcggtgacat catcaaatcc
    27421 atcgacgcta atcgccgccg aaccaccgtg acctgggaga taaacataca accgtgagta
    27481 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    27541 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    27601 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    27661 atttcaatta ccgtggtggt tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc
    27721 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    27781 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag
    27841 aaaatttgcc cgccgttgtt gttcaccaca attaatatca gcggcgcgga aacctgacgc
    27901 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    27961 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    28021 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    28081 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgctgatg
    28141 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc
    28201 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    28261 tccagccagt cggcaatatt ggcaattaag cggcgtccac gatggtgcgc cggatcaagc
    28321 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    28381 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    28441 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    28501 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt
    28561 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgtttttg tcgccagaag
    28621 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    28681 tcctgccacc agtcgcccag ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca
    28741 tacagcggtt cggcaaacgg gcagttgata tggactccac cagaatgaag cgtaccgaga
    28801 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    28861 gaaatactgt gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg
    28921 ccgcagtcaa tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    28981 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtaccgga ggtcacaatc
    29041 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    29101 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    29161 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    29221 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    29281 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    29341 tgcgactcat tactattcca tctgtaataa agtacgcagc cctgccgctt tgttgtcgat
    29401 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    29461 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa
    29521 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    29581 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    29641 taaacagatc acattatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    29701 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    29761 taaccgttgg cagatatctt ccaccaccag catattctcg cgctggtttt tatcatccgc
    29821 catcagccac tcacctaact gctgcgcctg cttatcatca ggatgatttg ctaccgttcc
    29881 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    29941 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    30001 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    30061 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    30121 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    30181 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg ccttaatcgc
    30241 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    30301 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    30361 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    30421 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    30481 ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg catcaaaagc
    30541 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    30601 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    30661 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    30721 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cgcccacacc
    30781 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg cctgcttcgc
    30841 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    30901 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    30961 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    31021 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    31081 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    31141 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    31201 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    31261 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgaccaat
    31321 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatatgcca ccagttcatc
    31381 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    31441 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    31501 ctgggaaacc gataattcag agtgatgcag atcttaccat tcaatcataa tgctctcctt
    31561 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    31621 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    31681 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    31741 ccagcatcag ctactgcata ccgcctttaa ccctgggaaa ctggacaaga tttttatcag
    31801 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    31861 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    31921 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    31981 tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact
    32041 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    32101 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    32161 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    32221 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    32281 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    32341 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    32401 cggcaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcaacacct
    32461 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    32521 taacgtttaa ctc
//
LOCUS       DAZOXR010000027        32478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZOXR010000027 DAZOXR010000000
VERSION     DAZOXR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.027"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            111..1484
                     /gene="aroP"
                     /locus_tag="ACHUNN_001078"
     CDS             111..1484
                     /gene="aroP"
                     /locus_tag="ACHUNN_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0575520.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1527..2381)
                     /gene="ampE"
                     /locus_tag="ACHUNN_001079"
     CDS             complement(1527..2381)
                     /gene="ampE"
                     /locus_tag="ACHUNN_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0575521.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2378..2929)
                     /gene="ampD"
                     /locus_tag="ACHUNN_001080"
     CDS             complement(2378..2929)
                     /gene="ampD"
                     /locus_tag="ACHUNN_001080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0575522.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3017..3910
                     /gene="nadC"
                     /locus_tag="ACHUNN_001081"
     CDS             3017..3910
                     /gene="nadC"
                     /locus_tag="ACHUNN_001081"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0575523.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4113..4553
                     /gene="ppdD"
                     /locus_tag="ACHUNN_001082"
     CDS             4113..4553
                     /gene="ppdD"
                     /locus_tag="ACHUNN_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0575524.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4563..5949
                     /gene="gspE"
                     /locus_tag="ACHUNN_001083"
                     /pseudo
     CDS             4563..5949
                     /gene="gspE"
                     /locus_tag="ACHUNN_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            5939..7141
                     /gene="hofC"
                     /locus_tag="ACHUNN_001084"
                     /pseudo
     CDS             5939..7141
                     /gene="hofC"
                     /locus_tag="ACHUNN_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7176..8219)
                     /gene="guaC"
                     /locus_tag="ACHUNN_001085"
     CDS             complement(7176..8219)
                     /gene="guaC"
                     /locus_tag="ACHUNN_001085"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0575525.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8375..8419)
                     /locus_tag="ACHUNN_001086"
     CDS             complement(8375..8419)
                     /locus_tag="ACHUNN_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575526.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8444..9064
                     /gene="coaE"
                     /locus_tag="ACHUNN_001087"
     CDS             8444..9064
                     /gene="coaE"
                     /locus_tag="ACHUNN_001087"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0575527.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9064..9807
                     /gene="zapD"
                     /locus_tag="ACHUNN_001088"
     CDS             9064..9807
                     /gene="zapD"
                     /locus_tag="ACHUNN_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0575528.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9817..10014
                     /gene="yacG"
                     /locus_tag="ACHUNN_001089"
     CDS             9817..10014
                     /gene="yacG"
                     /locus_tag="ACHUNN_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0575529.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10114..10503)
                     /gene="mutT"
                     /locus_tag="ACHUNN_001090"
     CDS             complement(10114..10503)
                     /gene="mutT"
                     /locus_tag="ACHUNN_001090"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0575530.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10563..13268)
                     /gene="secA"
                     /locus_tag="ACHUNN_001091"
     CDS             complement(10563..13268)
                     /gene="secA"
                     /locus_tag="ACHUNN_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0575531.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13330..13842)
                     /gene="secM"
                     /locus_tag="ACHUNN_001092"
     CDS             complement(13330..13842)
                     /gene="secM"
                     /locus_tag="ACHUNN_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0575532.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14073..14990)
                     /gene="lpxC"
                     /locus_tag="ACHUNN_001093"
     CDS             complement(14073..14990)
                     /gene="lpxC"
                     /locus_tag="ACHUNN_001093"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0575533.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15091..16242)
                     /gene="ftsZ"
                     /locus_tag="ACHUNN_001094"
     CDS             complement(15091..16242)
                     /gene="ftsZ"
                     /locus_tag="ACHUNN_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0575534.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16303..17565)
                     /gene="ftsA"
                     /locus_tag="ACHUNN_001095"
     CDS             complement(16303..17565)
                     /gene="ftsA"
                     /locus_tag="ACHUNN_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0575535.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17562..18392)
                     /gene="ftsQ"
                     /locus_tag="ACHUNN_001096"
     CDS             complement(17562..18392)
                     /gene="ftsQ"
                     /locus_tag="ACHUNN_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0575536.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18394..19314)
                     /gene="ddlB"
                     /locus_tag="ACHUNN_001097"
     CDS             complement(18394..19314)
                     /gene="ddlB"
                     /locus_tag="ACHUNN_001097"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0575537.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19307..20782)
                     /gene="murC"
                     /locus_tag="ACHUNN_001098"
     CDS             complement(19307..20782)
                     /gene="murC"
                     /locus_tag="ACHUNN_001098"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0575538.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20836..21903)
                     /gene="murG"
                     /locus_tag="ACHUNN_001099"
     CDS             complement(20836..21903)
                     /gene="murG"
                     /locus_tag="ACHUNN_001099"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0575539.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21900..23144)
                     /gene="ftsW"
                     /locus_tag="ACHUNN_001100"
     CDS             complement(21900..23144)
                     /gene="ftsW"
                     /locus_tag="ACHUNN_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0575540.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23144..24460)
                     /gene="murD"
                     /locus_tag="ACHUNN_001101"
     CDS             complement(23144..24460)
                     /gene="murD"
                     /locus_tag="ACHUNN_001101"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0575541.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24463..25545)
                     /gene="mraY"
                     /locus_tag="ACHUNN_001102"
     CDS             complement(24463..25545)
                     /gene="mraY"
                     /locus_tag="ACHUNN_001102"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0575542.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25539..26897)
                     /gene="murF"
                     /locus_tag="ACHUNN_001103"
     CDS             complement(25539..26897)
                     /gene="murF"
                     /locus_tag="ACHUNN_001103"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0575543.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26894..28387)
                     /gene="murE"
                     /locus_tag="ACHUNN_001104"
     CDS             complement(26894..28387)
                     /gene="murE"
                     /locus_tag="ACHUNN_001104"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0575544.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28374..30140)
                     /gene="ftsI"
                     /locus_tag="ACHUNN_001105"
     CDS             complement(28374..30140)
                     /gene="ftsI"
                     /locus_tag="ACHUNN_001105"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0575545.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30156..30521)
                     /gene="ftsL"
                     /locus_tag="ACHUNN_001106"
     CDS             complement(30156..30521)
                     /gene="ftsL"
                     /locus_tag="ACHUNN_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0575546.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30518..31459)
                     /gene="rsmH"
                     /locus_tag="ACHUNN_001107"
     CDS             complement(30518..31459)
                     /gene="rsmH"
                     /locus_tag="ACHUNN_001107"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0575547.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31461..31919)
                     /gene="mraZ"
                     /locus_tag="ACHUNN_001108"
     CDS             complement(31461..31919)
                     /gene="mraZ"
                     /locus_tag="ACHUNN_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0575548.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7712 a         9131 c         8016 g         7619 t
ORIGIN      
        1 gtaacaattc aaacttcttt gatgtaaaaa aattaataca acaaacggaa ttgcaaactt
       61 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
      121 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
      181 cgctgggtgg cgcgataggg acagggctat tcctgggtag cgcctccgta atacagtccg
      241 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
      301 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
      361 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
      421 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
      481 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
      541 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcga
      601 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
      661 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
      721 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
      781 cagcagaagc tgataacccg gagcaaagta tcccgaaagc gactaaccag gttatctacc
      841 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
      901 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
      961 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
     1021 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc
     1081 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
     1141 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
     1201 cactggtggt atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt
     1261 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
     1321 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
     1381 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
     1441 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
     1501 cccatgcagg gtgagggcaa taatctttac accaacgctc cgtaaatcgt cagtagcgca
     1561 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
     1621 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaacacc
     1681 tgatactgcg aagtatggaa gtcacccagc gaagcaaacc aggccggtaa cgctttctcc
     1741 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
     1801 tgaagcacgg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
     1861 cagtattgcc atgcacgcaa aaacgcatac cccatcagcg taacgggtcc ccaggttccc
     1921 cccacaatca accagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
     1981 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
     2041 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
     2101 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
     2161 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
     2221 gtcacgccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
     2281 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
     2341 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
     2401 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
     2461 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
     2521 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
     2581 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
     2641 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
     2701 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
     2761 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
     2821 taatattgtg caccaccagc agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg
     2881 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
     2941 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
     3001 tatctggagt tttaacatgc cgcctcgccg ctataaccct gacacccgac gtgacgagct
     3061 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
     3121 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaactctcg
     3181 ctctcatgcc acggtgagca cccgcgagaa tggcgtattt tgcggcaaac gctgggttga
     3241 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
     3301 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
     3361 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
     3421 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
     3481 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
     3541 ccgtctgggg ctttctgatg ccttcctgat caaagaaaat catattattg cctccggctc
     3601 agtgcgccag gccgtcgaaa aagcgtcctg gctgcacccg gatgcgccag tagaagtcga
     3661 agtagagaat ctggaagaac ttgatgaagc cctgaaagca ggagccgata tcatcatgct
     3721 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
     3781 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
     3841 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
     3901 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
     3961 caactttctt cgtaacgcct cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc
     4021 tcggaagctg ctttcccgtt tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa
     4081 agtagcgcca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
     4141 tcgaactgat ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt
     4201 atcaaaacta cctgcgcaaa gctgcactca ccgacatgct acaaaccttt gtgccttacc
     4261 gtacagccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
     4321 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaaag
     4381 gcgtggtgtc gctgaccggg caagaaagtc tcaatggact aagcgtcgtc atgacaccgg
     4441 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
     4501 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
     4561 caatgaatat tccacagctc accgccctgt gtctgcgtta tcagggagtc ttgctggatg
     4621 ccagcgaaga ggtggttcat gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg
     4681 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
     4741 aaggccacgc cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc
     4801 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgt gcgtctgata
     4861 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
     4921 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc
     4981 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
     5041 tggcaggaaa cgccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
     5101 tggtattaag gttgttacag caggtgaacc aggcactgga tgttaacacg ctgggaatgc
     5161 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
     5221 taactggccc tacaggcagc ggcaaaacgg tcacgcttta tagtgccctg caaaagctga
     5281 ataccgctga cattaatatt tgtagcgtcg aagatccggt tgagatcccc atagccggac
     5341 taaaccagac gcaaatccat ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt
     5401 tattgcgcca ggatcctgac gtcatcatga tcggagagat ccgctatggc tttaacggca
     5461 gaaattgcca ttaaagccgc gcaaaccggt cacctggtgt tgtctaccct acacactaat
     5521 tccacctgcg aaacgctggt acgtttacag caaatgggag tcgcccgctg gatgctctca
     5581 tcagcgctta cgctggtaat agcccagcgt ctggtacgta aactttgccc acattgtcgc
     5641 cagcagcaag gggagcccat ccatattcca gtcaatgtat ggccgtcgcc gctgccccac
     5701 tggcaggcac ccggttgtgt acattgctac cacggttttt atggtcgtac ggccttattt
     5761 gaagttctgc ccataacgcc ggtcattcgt cagcttattt ccgctaatac cgacgttgaa
     5821 tcgctggaaa cgcacgcacg acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg
     5881 gccgtggagc aaggcttaac cacctttgaa gagttaatcc gcgtactggg gatgccgcat
     5941 ggcgagtaag caactctggc gctggcatgg cataaccggc gacggcaatg cgcaagatgg
     6001 gatgctatga gcagagagcc gtgctttgct gctcatggca ctacagcaac agatggttac
     6061 cccacttagc ctgaagcgaa tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc
     6121 ggaagtcatt catcaactgg cgacgctact caaagccggg ttaacgcttt ctgaagggct
     6181 ggcactgctg gcggaacagc atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc
     6241 gcacgatctc gaacagggca ttgctttttc caatgcctta ttaccctggt cagaggtatt
     6301 tccgccactc tatcaggcga tgatccgcac gggtgaactg accggtaagc tggatgaatg
     6361 ctgctttgaa ctggcgcgtc agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc
     6421 agcgttacgt tatcccatca tcattttagc gatggcaatc atggtggttg tggcaatgct
     6481 gcattttgtt ctgccggagt ttgccgctat ctataagacc ttcaacaccc cactaccggc
     6541 actaacgcag gggatcatga cgctggcaga ctttagtggc gaatggagct ggctgctggt
     6601 gttgttcggc tttctgctgg cgatagccaa taagttgctg atgcgccgac cgacctggct
     6661 tatagtgcgg cagaaattgc tgttacgcat cccgattatg ggttcactga tgcggggaca
     6721 aaaactcacg cagatcttta cgattctggc gctgacacaa agtgcaggca ttactttttt
     6781 gcagggcgta gagagcgtca gagaaacaat gcgctgcccg tactgggtgc aacttctgac
     6841 acaaatccag cacgatatca gtaacggtca tcccatctgg ctggcgctaa aaaataccgg
     6901 ggagtttagc ccgctctgtt tgcaattagt gagaacagga gaggcatccg gctcgctgga
     6961 cctcatgtta gacaacctcg cccatcatca tcgggataac acaatggcgc tggcggataa
     7021 cctcgcagcc ttactggaac cgacgttgct gatcataacg ggaggaatta tcggtacgct
     7081 ggtggtggcg atgtatctgc caattttcca tttaggcgat gcgatgagtg ggatgggata
     7141 acgctggcgt gttgctccac accagcgttg gaagattaca ggttgttgaa gatgcggttt
     7201 tcttgttcct gcacacggat aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc
     7261 ccaacgtatg tacaagctga acgcaggccg cccaaaatat cacgcgcggt attttcaacc
     7321 gggcctcgca gcggcagctt aacggtttta ccttctgctg cgcgatattc cgcaacgccg
     7381 ccaacgtgac gtttcatcgc agactcggag ctcatgccgt agaacagcat aaatttctcg
     7441 ccgttctcct caacgatgcg accgccgctc tcttcgtggc ccgccagcat gccgccaagc
     7501 atgacgaaat cggcaccgcc gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca
     7561 ccgtcgctga cgatcattcc gcccagaccg tgcgccgcat cggcacattc gattaccgca
     7621 gaaagctgcg gataaccgac gcctgttttg acgcgagttg tacaaacaga acctgggcca
     7681 atgccaactt taacgatatc agcaccggag aggataagct cctcacacat ttcaccagtc
     7741 actacgttgc cagcacaaat ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac
     7801 tgcacgaagt gttcggaata accattcgcc acatcaatac aaacgaagtt taatgccggg
     7861 ttcaggtcga gaatctgttt agttttttcg aaatccgcat cagacgtacc ggtagaaacc
     7921 atcacatgtt tcagcacatc agcggaagaa ttgttgataa acgcttgcca ctcttcgaca
     7981 gaatagtgtt tatgcacagc agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa
     8041 aatgtaccta cggtgtccat atttgcggcg ataatcggca cgccggacca gctctgacct
     8101 gaatgtttga aggtgaattg acgttccagt tcaacatcgg aacggctttt aagagtggag
     8161 cgtttagggc ggatgagaac gtctttaaaa cctaacttca gatcttcttc aatacgcatg
     8221 tgcggattcc tggggttaat ggctataagg gtaagagcgc aactccagtg acgctatcat
     8281 acgcactaat caatgcggcg caagactgcg aaattgtctt tttttacgct acaatcccat
     8341 aaatttacct ggtaaatagt gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt
     8401 ttaacgtttt gataatcatg atgaattccc gggaatataa attatgaggt atatagttgc
     8461 cttaacggga ggcattggca gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg
     8521 aattaacgtc attgatgccg atattattgc gcgtcaggtg gttgaaccag gtgcacctgc
     8581 gctacatgcc attgctgatc actttggcgc taacatgatt gctgctgatg gaacattgca
     8641 gcgccgggcc ttgcgcgagc ggatcttcgc caacccggaa gagaaaaact ggcttaacgc
     8701 cctgctgcat ccgctgattc agcaagagac gcaacaccag atccagcaag ctacttcccc
     8761 ctatgtactg tgggttgtgc cattgctggt agaaaactca ctgtataaaa aagcgaatcg
     8821 agtgctggtg gtggatgtca gcccagaaac gcaacttaag cgcaccatgc agcgcgatga
     8881 tgtaactcgc gagcatgtcg aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct
     8941 tgccgtggca gatgacgtca ttgataataa cggcgcaccg gatgctatcg catcggatgt
     9001 tgcccgcctg cacgcacact atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc
     9061 gtaatgcaga cccaggtcct ttttgaacat ccactaaatg aaaaaatgcg tacatggctg
     9121 cgcattgagt ttttgattca gcaactcacc gttaatttac ccatcgttga ccacgctggc
     9181 gcgctgcatt tcttccgtaa tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc
     9241 cgcactgagc tgttgaaaga acttgaccgg cagcaacgta aactccagac ctggattggc
     9301 gtgcctggcg tggaccagag ccgtattgaa gcattaattc agcagttaaa agcggcgggg
     9361 agcgtattaa tttccgcgcc gcgtatcggg caatttctgc gtgaagatcg tttgattgct
     9421 ctggtgcgtc agcgactgag catcccaggc ggctgttgca gctttgattt acctacattg
     9481 cacatttggc tgcatctacc ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc
     9541 agcctgaacc cgctcaccca ggcacttacc atggtgctgg atttaattcg ccagtcggcc
     9601 cccttccgta aacaaaccag tctgaatggt ttttatcagg ataacggtgg cgatgccgac
     9661 ttgctgcgcc tgaatctgtc gctcgattca cagctttatc cgcaaatttc cggacataag
     9721 agccgttttg ccattcgttt tatgccgctg gacactgaaa acggacaggt accggaacgt
     9781 ctggatttcg aactggcctg ttgctaagga gtaaaaatgt cagaaactat tacggtgaat
     9841 tgcccaacct gcgggaaaac ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc
     9901 tccaaacgtt gtcagctgat cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc
     9961 agcagtggcg atctttccga aagcgatgac tggagcgaag agccaaagca gtgacatttg
    10021 tctgatgccg cacgtaggcc tgataagacg cggacagcgt cgcatcaggc atcttgtgca
    10081 aatgtcggat gcggcgtgaa cgccttatcc gacctacaga cgcttaagct tcgcaattac
    10141 cggttcattg gctggcggaa aatcatcggc attaagaccg actagcgaca tccactcacc
    10201 gggttgccct tctttacccc acggctcccc ttcccagctt tcgaccagcc agaaccacag
    10261 agttatatgc ctgtccggga attcatattc cagtttttca aatagcgaaa aatgttgtgg
    10321 ggtaatcccg acttcttcct gaagttcacg cactaccgcc tgttccggcg tttcgcccat
    10381 ttcaatttta ccgccgggaa actccagttt attcgccatg tgcgcatctg ctgcgcgacg
    10441 cgttataaag atttcattgt tctcgttgcg aataatacct accgcaattt gcagcttttt
    10501 cattgtctta aacctataaa aaaggcgcag aatcctgcgc cttttacttc aactgttagc
    10561 ttttattgca ggcgaccatg gcactgcttg tattttttac cagaaccgca cgggcaagga
    10621 tcgttacgtc ctactttgcg ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag
    10681 tcgtcatcct gatggctaag ctgctgcatt tgcgctaaac gctcggcttc catacgacgc
    10741 tgttgttcca gctcctcaac ctcttcaggc atacgtacct ggactttgct cagcgtgctg
    10801 ataacttcat attttaacga ctccagcatc gctgcaaaca tggagaacga ttcacgtttg
    10861 tattcctgct tcggatcttt ctgtgcatag ccacgcaggt ggataccctg acgcagatag
    10921 tccatcgccg ctaggtgctc tttccacaga gagtcgagag tttgcagcat gacgcctttt
    10981 tcgaagtgac gcatcatctc agcaccaacc acttcttctt tacgctgata cacttcgatg
    11041 gactgcgcca gaattcgctc acgcagcgtc tcttcatgca gttctggttc tttatccagc
    11101 cactcggcaa ttggcaaatc gaggtcgaaa tcgttcttca gacgttcctg cagccccgga
    11161 atatcccaca tttcttccag tgactgtggc ggaatgtagg catcaatggt cgctttgaac
    11221 acatcttcac gaatgctgtt gatggtttcg ctcacatcgc tgacatccag cagttcgtta
    11281 cgctgggagt aaatggcgcg acgctgatcg ttagccacgt catcatattc cagcagttgc
    11341 ttacgaatgt cgaagttacg gctttcaact ttacgctggg cgttggcaat cgctttagtc
    11401 acccacgggt gttcaatggc ttcgcctggc ttcataccca gtttacgcat catgccggat
    11461 actcggtcgg aagcaaaaat acgcatcagc gcatcttcca tcgacaggta gaaacgggaa
    11521 gaaccagcat ccccctgacg accagaacga ccgcgcaact ggttatcgat acgacgggat
    11581 tcgtgacgct cggtaccgat gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt
    11641 acctgccagt cggctttaat tttttcaatt tgctctgcgg tcggattttc cagcgcggca
    11701 acttctgcct gccagctacc accgagcaca atatctgtac cacgacccgc catattggtc
    11761 gcgatagtca ccgcagccgg ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg
    11821 aatttggcgt tcaggacgtt gtgcttaata ccggctttgg tcagttcgtt tgacaccagc
    11881 tccgattttt cgatggagat agtacccacc agcaccggct ggcctttcgc agtacgttct
    11941 ttgatatctt caatgatcgc ctgaattttt tccgcttcag tcatgtagac caggtccggc
    12001 agatctttac gaatcattgg acggttggtc ggaacaacga cggtatccag cttgtagatg
    12061 gagctaaatt cgaaagcttc ggtatcagca gtaccggtca tccccgccag tttttcatac
    12121 agacggaagt agttctggaa ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc
    12181 acaccttctt tcgcttccac agcctggtgc agaccatcgg accagcgacg gccctgcatg
    12241 gtacgaccgg tgtgttcgtc aacgatgata acttcaccat ctttaacgat gtagtcgacg
    12301 tcgcgggtaa acagcgcatg agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg
    12361 ttggccggag agtacagaga ctccccttca tccatgatgc cctctttcac cagcagttct
    12421 tcaatcagca ccagaccacg ttcggtcagg ttcacctggc gagatttttc gtccaccgag
    12481 aagtggcctt cgccctggaa ggtttcggag tcttcttttt cctgacggat caggtgcgga
    12541 ataattttat tcacgcgttt atacatttcc gagctgtctt ctgccgggcc ggaaatgatc
    12601 agcggtgtac gcgcttcatc gatcaggatg gagtccactt cgtccaccag cgcatagtgc
    12661 agtttacgct gtacacgttc ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag
    12721 ccgtattcgt tgttcgtacc gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt
    12781 gctggcatgc ccggcaggtt gataccgaca gtcaggccaa ggaattcaaa cagcggacgg
    12841 ttgttttcgg cgtcacgttg cgccaggtag tcgttgacgg taactacgtg cacgccttta
    12901 ccggttagtg cgttcaggta agcaggcagc gttgcggtca gggttttacc ttcaccggta
    12961 cgcatttcgg cgatgcagcg ttcgttaaga accataccgc cgagtaactg aacgtcgaag
    13021 tgacgcatac caaagacgcg cttacttgcc tcacgtacca cggcgaaagc ttccgggatc
    13081 agattttcca gcacttcgcc tttttccaga cgtgcacgaa actctgcggt tttccctttc
    13141 agttcttcgt cggagagttt ttccatctcc ggttccatgg cattgatgat gttgaccact
    13201 ttgcgcatcc ggcgcagggt gcgatcgtta cgactaccga aaactttagt taacaatttg
    13261 attagcataa taaaatctca aacgccccgc gttgcggagt taataaaatg aagtaaaggt
    13321 ttattgttgt taggtgaggc gttgagggcc agcacggatg ccttgcgcct ggcttatcca
    13381 gacgggcgtg ctgaattttg cttgtggggt aaaatgcgca taatcaatgc gataaccctt
    13441 ttcagacggc gtgccttcct gggtcagcag cgcgctgagc gtatccagta atgcaagatg
    13501 ttgcgcctga agaggcaaag actcttcagc aacgggcagt gtttgcggtg ccattgcgaa
    13561 agaaagatga cggattaccg tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt
    13621 cgaattcggg cggcgtgtgt tcgcttccag caaggccaat tgaccaaagt taactttggc
    13681 tgaaggctcg tggttgcggg ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct
    13741 gagcgcaggc aaacctaaac tcgccgcaac catccctaat aagagatgcg gccagaagta
    13801 gcgtttacca aactgtcgcc agcgcgtcag tattccactc acgttattgc catcccgatc
    13861 gtattcaaac gtatttagcg cacaaatctt atcattaaag cctgcagtcc acagcacgaa
    13921 tgaagataag ttgtgcggaa aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc
    13981 tcttataaaa ttcaaaaaaa aatgactggt tcacctggcc ggagagagtg ccagatttgc
    14041 cagtcgaatt ttatacgaca gtataaatgt cgttatgcca gtacagctga aggcgctttg
    14101 aaggccaacg gcagttctgc gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc
    14161 gccaggacag cctgcagcag tttgttattc agtgcatgac cggatttata agcggtaaat
    14221 gcaccaataa tattgtgacc acacatgaac aagtcaccga tcgcatcgag cattttgtga
    14281 cgcacaaatt cgtcttcaaa acgcaggccg tcttcgttca gtacgcgata atcgtcaaca
    14341 acgatggcac aatcgaagct gccgcccagg cacaaaccac gggactgcag atattcgata
    14401 tcacgcatga aaccgaacgt acgcgcacgg ctgatctggc gcataaacgc atcagcggag
    14461 aagttcatcg catagcgctg gttgctggaa tcaatagccg gatggttaaa atcgatggtg
    14521 aaatccagcg aaaaaccatt gtacggctta aattcagccc acttatcgcc atcttcgaca
    14581 cgaacagtct ctttgatgcg aacaaatttt ttggcgcagt tcaactcgtc gataccggcg
    14641 tcaagcagca ggtatacaaa cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg
    14701 ttaacttcga taacaatgtt atcgatgccc aggcccgcga gagcagcatt gaggtgctct
    14761 acggttgaaa tccgtacatc atgctcgttg accagacacg tacagagcat ggtatcacgc
    14821 acagatttgg catcggccgg gaaatctacc ggtggattca agtcggtgcg acgatagatg
    14881 accccggtgt tggccggcgc agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa
    14941 ccgacacccg tcgcctgaac gatacgttta agtgtccttt gtttgatcat cgtattatct
    15001 cgccaaatta cctatccaac cgaagtgtac tatacattcg gcgggccagt ttagcacaaa
    15061 gagcctcgaa acccaaattc cagtcaattc ttaatcagct tgcttacgca ggaatgctgg
    15121 gatatccaga taatccggct ctttcgcagt ttgcggcgca ttgtcattca cgactttagc
    15181 aaccggcttc tgctcctggg tcagcggagc catcccatgc tgctggtagc gatccatcac
    15241 tggctgctga acctgcttat tggtcaccag agtgatttca ggacgtttgt ccatgccgat
    15301 acctgtcgca acaacggtta cgcgcagctc gtcattcata tccgggtcaa gagaagtacc
    15361 gataaccaca gtcgcgttgt cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa
    15421 ctcatccaga cgcaggtcga agcccgccgt gatgttaacc agcacgccgc gcgcgccaga
    15481 caggtcgata tcttccagca gcggagaaga gatagccatt tcagcagctt cttccgcacg
    15541 gtcttcaccg ctcgccacgc cagaacccat cattgcgtag cccatctcag acattacggt
    15601 gcgtacgtct gcaaagtcca cgttcatcaa acccggacga gtaatcagtt cagcgatacc
    15661 ttgcacagcg cctttcagta catcgttcgc tgcgccaaac gcatccagca gggagatacc
    15721 gcggcccaga actttcagca gtttgtcgtt cgggatagtg atcagagagt ccacatgctt
    15781 ggacagttca gtgatcccct gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa
    15841 aggcttagtg acgacagcaa cggtcaggat acccaaatct tttgccactt cagcgacgac
    15901 tggtgctgca cctgtaccgg taccaccacc catacccgca gcaataaaga ccatgtctgc
    15961 accttccagc gccgcacgca atgcatcgcg atcctcatca gccgcattgc gaccaacttc
    16021 tggattagcg ccagcgccca gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc
    16081 aaccgctgtt ttacgcagcg cttgtgcatc ggtatttacc gcgaagaatt caacaccttc
    16141 aatacgctcg cgcaccatgt gttcaacagc attaccgccg ccgccgccga cgccgatgac
    16201 tttaatcacc gcgtcattgg taagttccat tggttcaaac atagtttctc tccgatttgt
    16261 gcctgtcgcc tgaggccgta atcatcgtcg gcctcataaa aattaaaact cttttcgcag
    16321 ccaactattg agtcgcttga tccacgagcc aactgatgct gtaacacgtt tttctacttc
    16381 agcttcaccg ttaagatgtg actctttccc atagtgaagc aatcccaccg ccgtcgaata
    16441 atacggctcc tgagcataat ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac
    16501 ttgcgtatga aacacgcgct gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc
    16561 ggttaataca atgcctgccg ccaggtgatg tttaacccct tgttggcgaa gcttttcctg
    16621 caactgcaat atctcttcgt tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac
    16681 ctctgccagg gtctgacgtt gcagactccg tggcggacga ccgcctacgc tcggcacttc
    16741 cacgctctca tcttttccaa cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc
    16801 ttcggcgtcg cttggcggcg tgccaaaggc gtaagcgata tcactggtca cgacattgcc
    16861 agcataagga attaccttag tgtggcgcaa tgccccaccg gtataaacgg cgatatccat
    16921 tgtaccacca ccgatatcga cgacgcagac acccagttca cgttcatctt ccgtcaatac
    16981 cgaataactt gatgccagtc cggcaaatat cagttggtca actttcagcc cacaacgttc
    17041 aaccgctttg acgatgtttt tcgccatatc gttgtgacat gtgatcaggt gcacttttgc
    17101 ctgcatccgc acgcccgaaa gtcctaccgg attcttgatc ccttcctgat agtcaatcgc
    17161 atactcttgc gggatcacat gcagcacacg atgttcatcg cgcacacgca ccgatttcgc
    17221 ggtatggacg acgttttcca catcttcttg cgtcacttct tcttcagaaa taggcaccat
    17281 accaatttca ttctggcagc tgatgtgctt accagaaagc gccagatata ccgaagagat
    17341 ctgacaatct gccatcaatt ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga
    17401 ttcgaggtcg ttcaccccgc ctttatccat accacgtgac gggcagctgc ccacgccaat
    17461 gatattgacc ataccgtcgg gcagaacttc ccctactaaa gcggcaacct tcgcggtacc
    17521 aatctccagt cctactacca gttttctgtc cgtcgccttg atcattgttg ttctgcctgt
    17581 gcctgatttt gttgctgagt agattcctct ggcggcaagg gcgcccagcc tactgccgct
    17641 ccagagtcat aacgcaaatc aacgtagcta atccgtttgc catcggtttg cgcctgctgc
    17701 tgtaaaaccg gataaagttc tacaaagcga gccaaacgtt tcatcgtatc gccccggcca
    17761 agattgagct taatatcgtt attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc
    17821 gcttccttca gagtaaatct gtcctttgcc agcatctgcc ccatttcgcg atagccctgc
    17881 aacacttcat tggcgctgcc ttccgggcca tacagcattg gaagcacctg cttgctggtg
    17941 cgatctggcg gcacgctgaa ggtgtttcct tccgcgtcta ccatatgttg atcattccac
    18001 cgcgcaatcg gcacatattc aaccagatga atcttcaatt catcaggcca ctgctttctg
    18061 acgctcacct gcttaatcca cggcaggcgt tgttctattt gcgtctggat gatgttgaca
    18121 tcctgggtca taaaggtacc cggctcaccc aatgccagga tcgactgccg gatatcgtca
    18181 ttacgcgtgt aatggcgttc accggtcaac accagctttg agagcggcag gcgttgcgca
    18241 tcttccatcc agcccaacac gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc
    18301 aggaaaagga tccccgccag acgcgttcca ttattgcggc gagaagaaac ctcttcttcg
    18361 ctatttcgcg tgttcagagc agcctgcgac atattagtcc gccagttcca gaattcgtac
    18421 taccaactgc gagaagctca tacctgcctg acgtgccgcc atcggcacca ggctgtggct
    18481 ggtcataccc ggtgaggtat tggcttccag cagataaaac tgtccatcgc tgtccagcat
    18541 aacgtcaata cgtccccatc ctttgcaacc taacgtcgtc catgctttca gcactaatgc
    18601 ctgcaaattg gcctcttgtg acgcttccag acctgcgggg cagaaatact gtgtctcatc
    18661 agagagatac ttcgcctcat aatcatagaa ggttccggac ggttgaatac gtattgacgg
    18721 taaaatttct tcaccgagta tcgcaaccgt gaactccggc ccacttagcc atttttcaat
    18781 caatacttct tcatcgtgct gaaatgccaa tcttaatgca tcttgtagag cattttctgc
    18841 tactactttt gacattccca cactggaacc ttcgcggctc ggcttaacga taaccggcaa
    18901 acccagagca gaaatttctg ctaactgctt atcgctcagg cctttttcaa actctgcgcg
    18961 ggttaacgct acccacggcg cgaccggtaa accggcacct tgccacagca gtttgctgcg
    19021 tagtttatcc attgaaagcg cagatgccat cactccgctg ccggtataag gcaagcccat
    19081 cagctcaagc atgccctgca gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa
    19141 cactttctga aaacccatcg acttcagttg cgtcacgtcg acttctttcg ggtcgacagg
    19201 atacgcatca ataccgcctt cacgcagccc ggctaacacc gctgcgccag aattcagaga
    19261 aacttcccgc tcagcggaag tcccgcccaa caggaccgca attttatcag tcatgttgtt
    19321 cttcctccgg agtttgcggc ttcagtttga tttcagctaa agaacgggca atttttccga
    19381 tattaccagc cccctgaacg agaatcaggt cgttaccgat taataccggt gccagcatct
    19441 cggctacctg cgcagggtcc ggcaccagaa tgggatcaat tttcccacgt ccacgaattg
    19501 tgcgacacag cgaacggctg tccgctcccg gaattggcgc ttcgccagcc ggatacactt
    19561 ccagcatcaa cagggtatca acctgcgtca gcacattggc gaaatcatca tacaggtcgc
    19621 gcgtacgggt aaaacggtgc ggctgaaaca gcattactag gtttttatcc ggccagcctg
    19681 cgcgcgccgc tttaatggtg gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca
    19741 gcattgccgt accgctttta ccattcactg gctccagcgg gaattcaccg aggaaatcaa
    19801 aacggcgacc agtcccctgg aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc
    19861 cctcttccgt agcaaccgca accgcagctg cggcgttcag cgcgttatga cggcctggcg
    19921 cattcagggt gacgcgcatc ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg
    19981 ggccaatctg ctgataatct tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg
    20041 tggtctgacg tcccacacgc ggtaacaatt cgcggatcac cggatcatca acacacatca
    20101 ccgcacgacc gtaaaacggc aggttgtgca gaaaattaat aaaagtctgt tttaaattct
    20161 caaagtcgcc ctggtaggta tccatgtggt cggcttcgat attggtgaca atcgccacca
    20221 tcggttgcag atgcaggaac gatgcatcac tctcatctgc ttcggcaatc aggtaccgac
    20281 catgccccaa acgcgcatga acccccgccg cttttaccag cccgccgtta acgaaggttg
    20341 ggtcgagccc cgcttctgcg tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt
    20401 gcgttcctgc aatggcgatg ccatgacgaa aacgcattaa ctcagccagc atttcggcac
    20461 gacggatcac cggaatacgc gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa
    20521 tggcgctgga aacaacgacc acgctggcat cacgtacgtt ttccgggcga tggttgaaat
    20581 aaatcgtcgc tcccagattc attaactgct gcgtgaccgg atttggcgct aaatcggaac
    20641 cactgatctg ataaccttca ttggccagaa cttcggcaat accgcccata ccggcaccac
    20701 cgatgccgac aaaatgtatg tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt
    20761 ttgccaattg ttgtgtattc attctttacg ccattaactt cttaaattca tacgatgcaa
    20821 aaggcatcgc tacaattacg cccgggcagc ccggctcact tcatttgcca ctcgctcggt
    20881 ggcatccgga atggatgcag cgcgggcgcg ttctgccatg gttaataagg tttctcgcga
    20941 ccacccggcc agggtgttgg cgacagcatc cacgctaagc tgtggctgct cgataatttt
    21001 ggctgcgccc gctttttcca gcggtagcgc attccagtat tgctggcggt ctttatgttg
    21061 aaacggcaca aacaacgccg gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc
    21121 ggagcggcaa acgacgacat ccgcccacgc atacgccgcc gccatatcat caataaattc
    21181 cgtcacttta tgctgcggtt gccccgcttc ggcatacgcc tgttcaacgg attgttgcga
    21241 acctttaccg ctctgatgcc agatagtgac tgaatcaccc agtttcgcag caacctgcgg
    21301 cattgtctgg ttaagaatgc gtgcgccctg ggaaccaccc actaccagca cacgaaccgg
    21361 accttcacgt ccagccaaac gttgctgcgg caacggcagc gccagcacat cggtacgcac
    21421 cgggttaccc accacttccg cattagggaa agcgcctgga aacgcctgca tcactttggt
    21481 ggcaatcttc gccagccatt tattggttaa gcccgcaata ccgttttgtt catgaagtac
    21541 aaccggaatg cctaacgacc acgcggccag accacctgga cctgatacat agccgcccat
    21601 accgagtacc acgtcaggtt tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt
    21661 gaagatacgc agcggcgcag ctatcagtgc ttttatacct tttccacgca gaccagagat
    21721 acgaatgaaa tcaatttcga tgccatgttt tggcactaag tccgcttcca tacggtcggc
    21781 agtccccagc cagcgaactt gccaaccctg agccattaga tggtgcgcaa ccgccagtcc
    21841 cgggaataca tgtccaccgg ttccgcctgc catcaccatt aatcgctttc cttgaccact
    21901 catcgtgaac ctcgtacaaa cgcctgcgct ttctccagac gcgtttcata atcaatacgc
    21961 aacagcatca tgatggctgt cgacataatc agtaagctcg aaccaccgta actgatcagc
    22021 ggcaatgtca gacctttggt cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc
    22081 tggaagctaa accagatgcc aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt
    22141 tctaacgctt tgcggccaat cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt
    22201 gccagcacca caccgacata ccccagttct tcgccgataa tggcgaaaat aaagtcagtg
    22261 tgcgcttccg gcagatactc cagtttttgt accgagttac ctaaaccttg tccccaaagt
    22321 tcgccgcgac caaacgccat cagcgattgc gttaactgat agccgctgcc aaagggatct
    22381 tcccacgggt tccagaatgc ggtaacacgg cggatacggt acggttcggc gagtatcagc
    22441 aacacaaccg ctgaaatgcc cataccgata atggcaatga actgccacaa tttcgctccc
    22501 gccaggaaca acatcgccag cgtagtcaca aacaacacca ccaccgtacc aaggtctggc
    22561 tgtgccagca gtaacactgc caacaccaga atcacgccca tcggtttcag gaagccgcgc
    22621 aggttattac gtacttcgtc gcctttacgc accagatagt tggcgatata acaaaacagc
    22681 gacagttttg tcagctccgc aggctggata cgcagcaaac cgagatcgat ccaacgcgat
    22741 gcccctttaa ccgagctacc cactaccagc acgatcatca gcaggatgat agatccgagc
    22801 agcatcgtgg cactgtagcg ttgccagaac tccatcggca gacgcagcgt aatgatcgcc
    22861 agaatgaatg ccaggatcag atagacgcca tcacgcttcg cgaagaagaa cggatcgttg
    22921 gttaagcgtt gccctatggg cattgacgcc gaggtcacca tgataaagcc aatcgccgca
    22981 aggccgaagg tcagccacag taaggtgcga tcgtacatga tcaggctgtc ggtatctttt
    23041 tcccgcgagc ccatcaccca gccttttagc gccgtggaga tccagaccag gatactgaat
    23101 cctggcaggc gcggcatttt caggcgaggg agagataaac gcatcaacct aactccttcg
    23161 ccagacgggc aaactcattg cctcgttgtt caaagttctt gaactgatca aggctggcac
    23221 aggccgggga gagcagaacc atatcgccca gctgaacacg cggagccagc aagcgcatcg
    23281 cttgttccat ggtttcggtt tgttctgcca cttccgggcg tagcgccgcc agctgcgcac
    23341 cgtcacgacc aaaacagtac aaacgcacgt tgtcgccatt cagatagcgc gccagtggac
    23401 tgaagtccgc cgatttacca tcgccgccca gcaacaaatg cagcgtgccg tctacctgca
    23461 agccattcaa cgccgcttcc gtactgccga cgttagtggc tttcgaatcg ttaatccagc
    23521 gtacgccatt gtgctccagc accacttcaa agcgatgcgg cagaccggtg aaggtggtta
    23581 acgctttcag gctgctggcg cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca
    23641 gcgcattggt gtagttatgc tgcccggaaa gtttcatctc tttcacattc agcactttct
    23701 cgcctttaac ccgcagccag gtttcgccct gctgatgatt caggtgatag tcacccatgt
    23761 tgacgccaaa gctgacgcag cgttcatcag caccgcgaat cggcattgtt aaggcatcat
    23821 cagcattgac gacacaaact ttcgcatttt cgtaaatacg caattttgcc gcacggtatt
    23881 gctgcaaacc aaacggatag cggtccatat gatcttcagt cacgttcaga atggtcgccg
    23941 ccaccgcctg taagctggaa gtggtttcca gctggaagct cgacagttcc agcacgtaca
    24001 gttcgcactc agcatcaagc agcatcaacg ctggcaggcc aatattgcca cccacaccaa
    24061 cgttaacccc cgccgctttc gccatttcac ccactagcgt ggtgaccgtg cttttgccgt
    24121 tagaaccggt aatcgccaca atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc
    24181 caacgatttc gattccggca tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac
    24241 cgggactggc gacaatcaga tctgccgcca tcagccattc atcattcaga ctgcccgtgt
    24301 ggcgttctac ggcttcgggt aatttatcca ggccaggcgg tgtcatacgc gtatccataa
    24361 cgcgcggcgt cacaccgcga gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca
    24421 ggccgataat gacgacattt ttaccctgat aatcagccat gattaacgta ccttcagcgt
    24481 tgccagacca atcagaacca gcatcagcga aataatccag aaacgcacaa tgacgcgcgg
    24541 ttccggccag cctttcagtt catagtggtg atgaatcggt gccatgcgga aaatacgttg
    24601 tccgcgcagt ttaaaggagc cgacctgcag gatgacagaa agcgtttcta ccacgaacac
    24661 gccccccata atcaccagca ggaattcctg acgtagcagt acggcgataa tgcctaacgc
    24721 accacctaac gccagcgaac ctacatcgcc cataaagacc tgcgccggat aggtgttaaa
    24781 ccacaggaag cccagtcctg ccccgactat cgccgtgcag acaatgacca gttcccctgc
    24841 gtgtcgcaga tacggtatat gcaagtagct ggcaaagttc atattgccgg tcgcccacgc
    24901 caccagcgca aaaccaccgg cgacaaatac ggtcggcata attgccaggc cgtcgagacc
    24961 atcggtcagg tttaccgcgt tgccagtacc cacaatgacg aagtaagcca gcagaatgta
    25021 gaacagcccc agctgcggca tcacatcttt aaagaatggg accaccagct gcgttgcggg
    25081 cgtgtctttg ccggcaaggt acagggcgaa ggcgacaccc agcgcaatga ccgacatcca
    25141 gaaatacttc caacgagcga tcaacccttt ggtgtcttta cgcaccactt tgcgataatc
    25201 atcaacaaag ccaataacac cgtaacctac cagcaccacc aacacgcacc agacgtacgg
    25261 attggacggg taagcccaca gcagtacgga gatcacaatc gccgtcagga tcataatccc
    25321 gcccatggtc ggcgtaccgc gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac
    25381 ctgaccaaag gaaagttttt gcaaatgagc tatcatacgc gggcccatcc acaatgagat
    25441 gaacagcgcg gtcagcaggc tgacgatggc gcgaaacgtc agataggaaa agacgttaaa
    25501 gccggaataa tatttgacca aatgttcggc cagccaaact aacatgtccc attctcctgt
    25561 aaagcgcgta ctacctcttc catggcggca ctacgtgaac ccttaactaa aatcgtaatt
    25621 acctgttgct cagcaatcag tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa
    25681 tgttcgccaa cgccgctggc ggtgctgata gcatggcttt gtttacccac gcttaacacg
    25741 cggtcaatac cagcagcttt tgccgcctcg cccacctgta catggcaggc ttcgctttca
    25801 gcgcccagtt ccgccatatc gcccaccacc agcacgcggt agcccggcat ttcagccagt
    25861 acctgaacag cggcggtcat tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac
    25921 tggttttctg ccagttggat ggggaacaga cggcctggaa cagctttcag atttgccagc
    25981 cccgctttga tagcatcaag cgttgcgccc acggacatgg agagcgcagc ggctgccagc
    26041 gcattcgcaa tattgtgacg ccccggcaac ggcagcagaa catcgacgct accggttggg
    26101 gtttgtaggg taaattccgt accgtgcgag gtcacatgga tattggtggc ggtgaaatcg
    26161 ctgttggcgg cattgggtga gaaacgccac actttgcgtg agccaattac gctctgccag
    26221 ttcagccagt cgttgttgtc ggcgttcata atggcgatac cgttttccgg caggccgcta
    26281 aagatttcac ctttcgcttt cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc
    26341 gctgccaggt tgttgaccag cgcagcttcc gggcgagtca gactcacagt ccaggctatt
    26401 tcgccctgat ggttcgcgcc aagttcaata actgcgtaat cgtattccgg cgtcaagcgc
    26461 aacaacgtca tcggtacacc gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg
    26521 ccgcactggc ttaaaatcgc cgccgtcatc tctttaacgg aggttttgcc ggaggaacct
    26581 gtcagagcaa ccacgcgcgc cggaacttgc tggcgaaccc atgcagccag ttcaccaaac
    26641 gccagacgcg tatccttgac gattaactgc ggcaggtcga tatccagcgg acggctgacc
    26701 agtagtgcgc ccgcgccgcc agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt
    26761 tcgcctttca gggcaacaaa caggcagccc ggcgtcagtt ttcgggtatc agtggttaca
    26821 gcatcaaggg tgatatctgc accttgcagt tcaccgttga gaatgtcggt aagttggcta
    26881 agggttacgc taatcatgca atcaccccca gcagacgcgc caccgtgacg cgatcggagt
    26941 agtccagacg ctgattgcca acaatctggt aatcttcatg gcctttgccc gcgaccagta
    27001 ccacatcatt ctctttagcc tgcataacgg cgcaagtcac cgcttcagca cggccttcca
    27061 tcactttggc atgtccggca tctaacattc ccgccagaat atcgttgatg atggcacgcg
    27121 gttcttcggt acgcgggtta tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa
    27181 ttgcgcccat cagtggacgc ttacctttat cgcgatcgcc accacagcca aagacgcacc
    27241 acagcttgcc cgcacagtgc aggcgcgccg cctgtaaggc tttttccagt gcatccggcg
    27301 tatgcgcgta atccaccacc accgtcggtt tgcctggcgc agtgaacact tccatacgtc
    27361 cgcaaaccgg ttgcagacgc gcggcggttt tcagcagatc agccagtgga tagccgagtg
    27421 ccagcagtgt cgccagcgcg agcagcaggt tgctgacgtt aaaagcgccc atcagatggc
    27481 tttcaatttc gccatcgccc caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat
    27541 agttcacgtc gatcgctttt aaccagcgtc cgtgacagtt cggattaata tgatcttcca
    27601 ttgataccgc aaccgcgtcc ggcagttttg ccagccagcg gcggcccact tcatcgtcgg
    27661 cgttaataat cgcctgaccg caatgatgtt cagaataaag cagccatttc gcagcttcgt
    27721 agtgttccat atcaccatga taatcaaggt gatcgcggct taagttggta aagaccgacg
    27781 ccgcaaattt caatgccgcc acacggtgct gtaccagccc gtgggaggaa acttccattg
    27841 cgcaaaacgt cgcgccctgc tccaccagcc ccgccagctc atgctgaaca tcgactgcgg
    27901 aaccggtagt attttctgtc gggatcactt tcccaagcag accgttacca acggtgccca
    27961 ttaccgcgct ggtttcgcca agcagttggc tccactgcgc cagcagctgg gtagtcgtgg
    28021 ttttgccgtt ggtgcccgtt acgcctacga gacgtaaatt gtcagagggt tcatggtaaa
    28081 agcggcccgc cagtgcagat aaacgctcat tgagctggct gagatagatg accggtacgc
    28141 cgtgcatttc acggatttca ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa
    28201 tggcagccac accttgcgct atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac
    28261 ctactacagc tacaaagaga tcgcccgccg cagccacacg gctgtcgagt gtcatctctc
    28321 gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag aaggtcgcgc aaattacgat
    28381 ctgccacctg tcccctcgcc ttgattaatc acaaattcat ttttatcgcc cgttgtcagc
    28441 gcatccggct cgatgttcat ggtacgcaat acgccgccca tgatggcacc aaagaccggc
    28501 gcggaaacgg cgccgccgta gtatttaccc gcctgcggat cgttgataac aacaaccagc
    28561 gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat aagcaatata tttattgatg
    28621 tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt taatggcgat acgatagcct
    28681 ttaatcgccg ccttcacgcc gccgccgcct ggtagcgcca cgctttccat catatgcacc
    28741 acagtgcgga caatggatcc cgggaagaca cgttcaccgg gaaccggggg gtcaactttg
    28801 gtaatcgaca gtgggcgata aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac
    28861 tgtaacggtg ttaccattag cccgtagccg aaagagaagg tggccctctc tatgtcagac
    28921 caccgttgtt tttgaggata taagccactg cgttctccga ccaaccccaa attggtcgct
    28981 tttcccagtc caaaacgtga gtaagtatct actaacgctg aggacggcat cgctaacgcc
    29041 agcttggaaa caccgacgtt actcgacttc tgtaataccc cggtcagggt taattcgctg
    29101 tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat aaggaatggt attgagtacc
    29161 gagttttccc gcaccacgcc acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt
    29221 gagcccggtt caaacacgtc ggtgatggta cggttacgca tcgcctcttt cggcgtgccg
    29281 ctcagattgt tagggttgta tgacgggctg ttagccatcg ccagcacttc accggtgttg
    29341 acatccacca gcacggcgct accagattca gccttgttaa aggccaccgc gttgttcagt
    29401 tcgcgataaa ccagcgcctg caggcgttca tcaatactca gcgccaggtt gtgcgctgcc
    29461 tggctgtcag tagaagaaat atcttcaatt acgcgaccat agcggtcttt acgcacaatg
    29521 cgctcacccg gctgcccggt aagccattta tcgaaactct tctcaacgcc ctcaatccct
    29581 tgactatcga cgttagtaaa gccgatgagg tgagcagtca cttcgccgga cggatagtaa
    29641 cggcgagact cttcacgcag atgaatcccc ggcagtttca gttttttgat gtagtccgcc
    29701 atgtcagggt tcacctgacg cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg
    29761 cgggctgaaa gctgatccag cggaatattg agcgcgttag ccagcgcctt ccagcggtca
    29821 ccgacgctga taccgccagc gtcatgcact tctttcgggt cagcccaaat cgcttttacc
    29881 ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa
    29941 acttgctgaa cgcgaagaga acgcatgtcg ccctctttca ccagcatatc cggggagata
    30001 acttgtaacc acgctacgcg tccgagcaga aaagccagcg ccaggagaat acagccgcat
    30061 aacaacgcaa aacgccaact gataaagttg gcatgttctt cctgacgttt tggtttctgc
    30121 gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt tttgcactac gatattttct
    30181 tgtgacggat caacatgctg catttgcagc ttttccgtgg cgatcctttc cacccggcta
    30241 tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc attcaatgtc taaagcatct
    30301 cgctccagca ccagttgttc gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt
    30361 accacagtca ccgccgtcaa aataatgcaa atgaacaggc agagtggcag cttcccaaat
    30421 cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc gctcgtggct tcccatcgat
    30481 cctttaactt tgcttagagc ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa
    30541 tacgcagaac tgaactacgg gcacgagggt tctcagccac ctcttcttcg cccggcatta
    30601 acttgcctag tgctcgcagc tgacggccac ccagtttttt gagctgctct tcagtcatcg
    30661 gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg cataaaacgt ttcacaatac
    30721 ggtcttccag cgagtggaag ctgatgatcg aaagccgccc acccggggcc agcacgttga
    30781 gcgagctttt tagcgcctgc tctatctcct ccagttcact gtttacccaa atgcgcaccg
    30841 cctggaaggt acgggtcgcg ggatgtttaa acttatcttt caccggcgtt gcaacagcca
    30901 cgacttccgc cagttctttg gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg
    30961 cgcgggcaat gcgtttggca aaacgctctt caccataggt tttcaatacc caagcgatat
    31021 cggcttcttc tgcggtttgt agccattcag cggctgactg cccacgggtt gggtccatac
    31081 gcatgtccag cggaccatcg cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg
    31141 gtgaagagac gccaagatcg aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg
    31201 caacgtattc gcccagcgcg gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa
    31261 tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt
    31321 cgccaagctg cgagaggatc agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt
    31381 agatgccatc aggacggata ttgaggccat taacggcttc atccagcagc accgtagtat
    31441 gtttatagtt ttccatcatt ttatagagac aagtcctgca gtcgctccga taagtctccg
    31501 gtagccaact gctctgcgtc gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc
    31561 cacagctcaa acttgttgaa ctgtccaacc agcatcactt cttttgtcag cccggcatgt
    31621 tgccgcagta ctggcgcgat taacaatcga cctgcgccat ccatctgaca ttcgctggca
    31681 tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt tcatgctcga cagacgcgat
    31741 aatttttgct cgataatttc ccattcaggc agggggtaaa gcagcaggca cgggtgatga
    31801 atgtcaatgg tgcaaaccat ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg
    31861 gtaggcactg ataagcgccc tttgctgtcg agattgacta acgttgctcc ccggaacatg
    31921 ccagcctcac cccttattcc cactttgccc cacaatttcc cactgaaagg agtttacgga
    31981 gcggaggaaa agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa cccgatgttt
    32041 acggctaata tcagcgttgt ttattttacg tccaacgtgc gcagcaaatt aacgttatga
    32101 atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt aaaatgactc actattttcc
    32161 acagcaaata taaagtgtca gtttgcgacg cgagcggcat tttaggacat atcttccccg
    32221 gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa
    32281 agaaaagtgc ttgttaaatc ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg
    32341 cgctgtcgat atcacttaat tattgtgaat ttcagctggt gagttaatta acgggcgggt
    32401 ttaaaagaac ttatcaataa tagtcaggaa tagtcttatt tactttaagg atattgatgt
    32461 ccattcccgt actctacg
//
LOCUS       DAZOXR010000028        32432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZOXR010000028 DAZOXR010000000
VERSION     DAZOXR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.028"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            190..753
                     /locus_tag="ACHUNN_001109"
     CDS             190..753
                     /locus_tag="ACHUNN_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575549.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            835..3477
                     /gene="yfcU"
                     /locus_tag="ACHUNN_001110"
     CDS             835..3477
                     /gene="yfcU"
                     /locus_tag="ACHUNN_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFR0575550.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3497..4248
                     /locus_tag="ACHUNN_001111"
                     /pseudo
     CDS             3497..4248
                     /locus_tag="ACHUNN_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4223..4771
                     /locus_tag="ACHUNN_001112"
     CDS             4223..4771
                     /locus_tag="ACHUNN_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575551.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4768..5238
                     /locus_tag="ACHUNN_001113"
     CDS             4768..5238
                     /locus_tag="ACHUNN_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575552.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5235..5759
                     /locus_tag="ACHUNN_001114"
     CDS             5235..5759
                     /locus_tag="ACHUNN_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575553.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5746..6618
                     /locus_tag="ACHUNN_001115"
     CDS             5746..6618
                     /locus_tag="ACHUNN_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0575554.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6740..7291)
                     /gene="smrB"
                     /locus_tag="ACHUNN_001116"
     CDS             complement(6740..7291)
                     /gene="smrB"
                     /locus_tag="ACHUNN_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0575555.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7457..8389
                     /gene="prmB"
                     /locus_tag="ACHUNN_001117"
     CDS             7457..8389
                     /gene="prmB"
                     /locus_tag="ACHUNN_001117"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0575556.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8424..9509
                     /gene="aroC"
                     /locus_tag="ACHUNN_001118"
     CDS             8424..9509
                     /gene="aroC"
                     /locus_tag="ACHUNN_001118"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0575557.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9513..10337
                     /gene="mepA"
                     /locus_tag="ACHUNN_001119"
     CDS             9513..10337
                     /gene="mepA"
                     /locus_tag="ACHUNN_001119"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0575558.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10337..11146
                     /locus_tag="ACHUNN_001120"
     CDS             10337..11146
                     /locus_tag="ACHUNN_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR0575559.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11146..11694
                     /gene="epmC"
                     /locus_tag="ACHUNN_001121"
     CDS             11146..11694
                     /gene="epmC"
                     /locus_tag="ACHUNN_001121"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0575560.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11728..12006
                     /locus_tag="ACHUNN_001122"
     CDS             11728..12006
                     /locus_tag="ACHUNN_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0575561.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12127..14133)
                     /gene="mnmC"
                     /locus_tag="ACHUNN_001123"
     CDS             complement(12127..14133)
                     /gene="mnmC"
                     /locus_tag="ACHUNN_001123"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0575562.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14292..15512
                     /gene="fabB"
                     /locus_tag="ACHUNN_001124"
     CDS             14292..15512
                     /gene="fabB"
                     /locus_tag="ACHUNN_001124"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0575563.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15787..16965
                     /locus_tag="ACHUNN_001125"
     CDS             15787..16965
                     /locus_tag="ACHUNN_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575564.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16962..17957)
                     /gene="flk"
                     /locus_tag="ACHUNN_001126"
     CDS             complement(16962..17957)
                     /gene="flk"
                     /locus_tag="ACHUNN_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0575565.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18056..19192
                     /gene="pdxB"
                     /locus_tag="ACHUNN_001127"
     CDS             18056..19192
                     /gene="pdxB"
                     /locus_tag="ACHUNN_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0575566.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19258..20271
                     /locus_tag="ACHUNN_001128"
     CDS             19258..20271
                     /locus_tag="ACHUNN_001128"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0575567.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20271..21083
                     /gene="truA"
                     /locus_tag="ACHUNN_001129"
     CDS             20271..21083
                     /gene="truA"
                     /locus_tag="ACHUNN_001129"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0575568.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21166..21825
                     /locus_tag="ACHUNN_001130"
     CDS             21166..21825
                     /locus_tag="ACHUNN_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0575569.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            21981..22895
                     /gene="accD"
                     /locus_tag="ACHUNN_001131"
     CDS             21981..22895
                     /gene="accD"
                     /locus_tag="ACHUNN_001131"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0575570.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22965..24233
                     /gene="folC"
                     /locus_tag="ACHUNN_001132"
     CDS             22965..24233
                     /gene="folC"
                     /locus_tag="ACHUNN_001132"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0575571.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24223..24885
                     /gene="dedD"
                     /locus_tag="ACHUNN_001133"
     CDS             24223..24885
                     /gene="dedD"
                     /locus_tag="ACHUNN_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0575572.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25144..25632
                     /gene="cvpA"
                     /locus_tag="ACHUNN_001134"
     CDS             25144..25632
                     /gene="cvpA"
                     /locus_tag="ACHUNN_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0575573.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25669..27186
                     /gene="purF"
                     /locus_tag="ACHUNN_001135"
     CDS             25669..27186
                     /gene="purF"
                     /locus_tag="ACHUNN_001135"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0575574.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27281..27850
                     /gene="ubiX"
                     /locus_tag="ACHUNN_001136"
     CDS             27281..27850
                     /gene="ubiX"
                     /locus_tag="ACHUNN_001136"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0575575.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            28116..28898
                     /gene="argT"
                     /locus_tag="ACHUNN_001137"
     CDS             28116..28898
                     /gene="argT"
                     /locus_tag="ACHUNN_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0575576.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            29119..29901
                     /gene="hisJ"
                     /locus_tag="ACHUNN_001138"
     CDS             29119..29901
                     /gene="hisJ"
                     /locus_tag="ACHUNN_001138"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0575577.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            29991..30677
                     /gene="hisQ"
                     /locus_tag="ACHUNN_001139"
     CDS             29991..30677
                     /gene="hisQ"
                     /locus_tag="ACHUNN_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0575578.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30674..31390
                     /locus_tag="ACHUNN_001140"
     CDS             30674..31390
                     /locus_tag="ACHUNN_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0575579.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31398..32171
                     /gene="hisP"
                     /locus_tag="ACHUNN_001141"
     CDS             31398..32171
                     /gene="hisP"
                     /locus_tag="ACHUNN_001141"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0575580.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            32368..>32432
                     /locus_tag="ACHUNN_001142"
     CDS             32368..>32432
                     /locus_tag="ACHUNN_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575581.1"
                     /translation="MTISTTSTPHDAVFKSFLRHP"
BASE COUNT         7611 a         8021 c         8760 g         8040 t
ORIGIN      
        1 aaaattaaat tgcatcaggg gtgtggaaac atcactatgc ggccccacac caacgaatac
       61 aattttctac gacctgttct ttttggatcg catgccgtat tcaatggctg ggtataaaca
      121 ttggttattg actgaatcaa tgtattgcaa tgtttatatt tttaattatt tatagtttaa
      181 ggacaaataa tgagtaagtt tgttaaaaca gctattgctg cggcaatggt gatgggcgtg
      241 ttcacctcta cggcaacaat cgctgcgggt aacaacggta ctgcacgttt ttacggcacc
      301 attgaagact cagtgtgttc tatcgttccg gacgatcaca aactggaagt tgatatgggt
      361 gatatcggtg ccgaaaaact gaaaaataac ggcaccacca cgccgaaaag tttccagatt
      421 cgtctgcaag attgcgtatt tgatactcag gaaacaatga ccactacctt tactggtacc
      481 gtttcttctg caaatagcgg caattattac accattttca ataccgatac ctgtgcggca
      541 tttaacaatg ttagcctggc gatcggtgac tctctgggta cctcttacaa aagcggcatg
      601 ggtattgacc agaaaatagt gaaagacact tctaccaata aaggcaaagc gaagcagaca
      661 ctgaacttta aagcctggct ggtcggcgca gctgatgcgc cagatctggg taatttcgaa
      721 gcaaatacca ccttccagat tacttacctg taaggcatac ccccatagcg acactgctat
      781 gggggattta aatggatttt gaccgaacaa gaataaaaac ggatagaaac gtgtatgcct
      841 gaccattctc tttttcgatt acggattctt ccgtggtgca ttgcgctggc aatgtcaggg
      901 agttatagca gtgtctgggc tgaagacgac attcagtttg attcccgttt tctggaatta
      961 aaaggcgaca caaaaattga tctgaagcgt ttttccagcc agggatatgt tgagcccgga
     1021 aaatacaatt tacaggttca actaaataaa cagccattgg cggaagagta cgatatttac
     1081 tggtatgctg gtgaagatga cgcgagcaaa agctatgctt gtctgacacc ggaactggta
     1141 gcgcagtttg gtttaaaaga agacgtggcg aaaaatctgc aatggagcca cgatgctaaa
     1201 tgcctgaaat ccggtcaact ggaaggcatg gaaattaagg ctgatttaag ccagtccgca
     1261 ttagtcattt cactgccaca ggcttacctc gaatatactt atcccgactg ggatccgcct
     1321 tcacgttggg atgacggcat ctccgggatc gtcgcggact acagcatcaa cgcacaaacc
     1381 cggcacgaag aaaatggcgg tgatgatagt aacgagatca gcggcaacgg gacggtcggg
     1441 gttaacctgg ggccgtggcg tatgcgtgct gactggcaga ctaactatca acatactcgc
     1501 agtaatgatg acgatgaatt cagcggcgat gaaactcaaa aaaaatggga gtggagtcgc
     1561 tactatgcct ggcgggcgtt accatcatta aaagccaaac tggcgctggg cgaggattac
     1621 ctcagatccg atatttttga tggttttaac tatgttggtg gcagtgtcag tactgacgat
     1681 caaatgttgc ctcccaatct gcgcggctac gcgccagaca tttccggcgt ggcacacacc
     1741 acagcaaaag tgaccgtcag ccagatgggg cgtgtgattt acgaaacgca ggttccggct
     1801 ggaccgtttc gtattcagga tcttggtgat tctgtctccg gtacgttgca tattcgcatt
     1861 gaagaacaga acggccaggt gcaggaatat gacatcagca ccgcctcgat gccatacctc
     1921 actcgtccag gtcaggttcg ctataagatc atgatgggcc gtccgcaaga gtggggacac
     1981 catgtcgagg gtgaattttt ttctggtgct gaagcttcct gggggatcgc taacggctgg
     2041 tcgttatatg gcggcgcact gggagatgaa aactatcagt ctgcggcgct tggcgtcggt
     2101 cgcgatttgt ctacattcgg cgcggtcgcg tttgatgtta ctcactcgca caccaaactg
     2161 gataaagaca ccgcttatgg caaaggttcg ctggacggta actccttccg tgtgagttat
     2221 tccaaagact ttgaccagct caacagccgc gttacttttg ctggatatcg cttctcggaa
     2281 gagaacttta tgaccatgag cgagtatctg gatgccagtg acagcggaat ggtacgcacg
     2341 ggcaacgaca aagagatgta cactgccact tataaccaga acttccgcga tgcgggtgtt
     2401 tcggtttatc tcaactatac ccgccatacc tactgggatc gcgaggagca gacaaactac
     2461 aacatcatgc tctcgcacta tttcaatata ggcagtattc gtaatgtcag catctcgatg
     2521 actggctacc gttacgagta tgacaaccag gccgacaaag gcatgtacat ttcgctcagt
     2581 atgccgtggg gtgacaacag taccgttagc tataacggta actatggcag tgggacggac
     2641 agcagtcagg tcggttattt cagccgtgtc gatgacgcga ctcactatca gttgaacgtc
     2701 ggcaccagtg acaaacacac cagcgttgac ggctattaca gccatgatgg ttcgctggcg
     2761 caggttgacc tcagtgcgaa ctaccatgaa gggcaataca cctctgcggg cttgtcgtta
     2821 cagggcggcg caacgcttac tgcccacggt ggcgcacttc accgtaccca gaatatgggc
     2881 gggacacgct tgttgattga tgccgatggc gttgccgatg ttccggtaga aggtaacggt
     2941 gctgctgttt ataccaatat gtttggtaaa gccgtcgttt ctgacgtcaa taactattac
     3001 cgcaatcagg cgtatatcga cctcaacaaa ctgccggaaa acgccgaagc aacccagtcg
     3061 gtggtacaag ccacgctaac tgaaggggcg attggctacc gcaaatttgc cgtcatcagt
     3121 ggtcaaaaag cgatggcggt gctgcgttta caagacggca gccatcctcc gtttggcgca
     3181 gaagtaaaaa atgataacga gcagacagtg ggccttgtcg atgatgacgg caatgtttat
     3241 ctggctgggg tgaaacctgg cgaacacatg agtgtgttct ggagtggtgt tgcgcattgc
     3301 gatatcaacc tgccggaccc gctgcctgcc gatctgttta acggcttgtt actgccatgc
     3361 cagcataaag gcaacgtcgc gccggttgtt cctgatgaca taaagcctgt cattcaggag
     3421 cagacgcaac aggtgacacc caccaatccc cctgtttctg tttcagctaa ccaataacgt
     3481 gattaaggaa tgatccatgt ctgatttact ttgctctgcc aaattgggtg ctacgacgct
     3541 agcgttactg cttagcgctg ccagtttgtc gcccaggctt ctgtaacgcc agatcgtacc
     3601 cgtttgatat ttaatgaaag cgacaaatca atcagcgtga cgttgcgcaa taacgatccg
     3661 aaactgcctt atctggccca aagctggatc gaagatgaaa agggtaacaa gatcagttca
     3721 ccgttaactg ttctgccacc ggtgcaacgc atcgattcaa tgatgaacgg gcaggtaaaa
     3781 gtgcagggaa tgccggatat caacaaattg cctgccgatc gtgaaagcct gttctacttc
     3841 aacgtgcgtg agatcccgcc aaaatcaaac aaagccaaca cgctgcaaat agcactacaa
     3901 acgcgtatta agttgttctg gcgaccgaag gcgctggaga acgtcagcat gaaaaacccc
     3961 tggcaatata aggttacttt aacccgcaac ggtcaggaat ttaccgtcaa taacccgacg
     4021 ccgtattacg tcattttcag taatgccagc acacagaaaa atggcaatcc tgcagccggt
     4081 ttctcgccaa tagtgatgtc gccgaaaacc tctgcaccgc tgaatgtgaa gatgggtagc
     4141 gtgccggtac tgacctatgt caacgactat ggcgctcgga tgccgctctt tttcacttgc
     4201 aacggtaaca gttgccaggt cgatgaagag cagagccgca aaggctgagg ccgggagagc
     4261 cagtatgaaa ggatcttgtt ctgtctgggc ggtaactggg ttactgatat gttgtgttgg
     4321 tagcggcttt gcagttgcag cgagtaagtc agtaagtatg aatttacggg tgcttgtcga
     4381 tgcccctcct ccttgtacgg taaatggtgc ggcagttgag tttggtaatg tattcattaa
     4441 taagatcaat ggggttgatt ataaacgtcc cattgactac tcgcttgttt gtaacaatct
     4501 ggcaatggat gacctgcgtc tacagatgca ggcgacaacg gtcgtgatta acggtgaaac
     4561 agttatcagc acgggtattc ctgggtttgg tattcgggtt cagaaaagta gtgatcacac
     4621 aatactcgac ttaacctctg gctcatggtt gccatttaat ttttcctcgg gagttcccgt
     4681 tctggaagct gtgccggtga aacagagtgg tacaacgtta gcggcagctg aatttaacgc
     4741 cagtgccaca attgtggtgg attaccaatg aaacggatct cgcttatttt gctgtggggc
     4801 ttttgctcga tggcactctc aaacgtctct tttcacggtt atttagtgca gccgcctaac
     4861 tgcacgatta gcaacgctca aaccattgaa ataacttttc aggatgtgct tattgatgac
     4921 attaatggca gcaattatga gcaaaccgtg ccgtattcca ttacctgcga tacggcggtt
     4981 cgcgacccat taatggaaat gaccttgagc tggagtggca cactaagcga ttttgataat
     5041 gctgcagtaa gcagcaatat cactggttta ggcattcagc tcaaacaggc agggcagagt
     5101 tttacgatca atacgccact tatggtgaat gaaaccgatt taccggtgtt aacggctgtg
     5161 ccagtaaaaa agagtggtgt cgttctgcca gaggcggact ttgaggcctg ggccacgtta
     5221 caggtagatt accaatgaag aaaaagcgta cgttgttttt tattagtagc ctgatgttgc
     5281 tgggcagtgg aaccacaata gcaggagata atttgcactt taccggcaat ttaatcagta
     5341 aatcgtgtac gccagtgatt aatggtagcc agttggcaga agtacatttt cctgccattg
     5401 ccgccagcga tttaatgaac cttgggcagt cagagcgagt gccattggtg tttcaattga
     5461 aagattgtca tagttctaca ttgtttaatg tcaaagtaac gttgacagga acagaagata
     5521 gtgctctacc cggctttctg gcctttgact cctcgtcatc agcatccggc gctgggattg
     5581 gtattgaaac tgcggcaggc acatcggtgc caatcaacaa tacaacaggc gtcacgctcc
     5641 cgctgaatca gggaaataac agcctaaatt ttaatacctg gttacaggca aagagcgggc
     5701 gagatgtaac gtccggagac tttagtgcca cagtgacggc aacctttgag tatttctgaa
     5761 atgaaaaaat ggaccataat tttcgcgagc ctaatgctgc tcgttctgag tgttgtcggc
     5821 gcatctaaga gttatgctgg agataaactt atgagtgcga gttttgacag tgtgagagtt
     5881 tattatgcca tggacaaggt aaccggtgct gtagcgagca gtgttttcaa tgttacggtg
     5941 ataacgccaa aggaggttac atatgggaaa tatgattctt ttgcgtataa aggcgatacg
     6001 ttgcgggtta tttcctggtc tgggtcgggt tcagctccaa ctttggtact gactgacttt
     6061 gatacaatta ataactcaaa ttgccctggc atagatacga aaatttttcg ttgtgcatat
     6121 atgacgttta aaattaccgt tgcatctgat gattatggtt gcccttggat cgcatccttt
     6181 tattcctata cagatttacc cggatttgga agctatacgg cccctacagt gcataacaca
     6241 atatgcccaa cgataccggt cacttcatac gatatctcct ggagtgaaaa ttatgtcagc
     6301 cataataagg cgttacgaat tcagtccacg gggtcaacag tgaccaccac gctttcgacc
     6361 tacctgatgg aaggcggcag actctgtgat ggcagtaatt tttctgataa tgatggcaga
     6421 ggagcatact gccgcgcggt atcagaattg ttaaccttta cctcttacgg ttgtgataaa
     6481 tcaacggtaa cggttacgcc aactcgtcat ccggtaacgg ataaagtact acacgatatt
     6541 gtggtgaatg tgaataccag tagtgggcaa ccgattgatt cgacgtgtcg ttttcagtat
     6601 gtattgaatg aactgtagtt cgtagtattg ctctgatgcg tcttatcagg cctacgaagt
     6661 gaattgcaat ttattgaatt atcacatatt tgtaggccgg ataaggcgtt tacgctgctc
     6721 cggcatttgc tgccagaatt cagggcaact ccggcggcaa ccactcttcc acttcaatca
     6781 acaccaacaa agccgcatca ccgccgtact ctttcggtgc ctggtgaaat gccatcacat
     6841 gcggatgttg tgccagccac agcggtgttt gttgcttcaa aatatgcttc ccgtgcccat
     6901 gcatcacgca ggcacaaaac acatgttcgc ggcgacaggc ggcaatcaac gcgcccagtt
     6961 cctgcttggc ctgcagctgc gtcagaccgt gtaaatccaa aaacaattcc ggtgaataat
     7021 cgccgcggcg cagtttcttc gcctcaaaat ggctgacatc cgggcgaaca tatttcaccg
     7081 gaccttcggt atttaataac ggctgaaact catcggagaa ataatggctg gcatcagcct
     7141 gctccgggat caagcgtttc accggcactt cgctgatttt tttacgctgc ggtcggtgga
     7201 caatcgtgtc ctgcttaatc ttgcgagtcc ccgccatcaa ctggcgaaac agagcctggt
     7261 cctcctcgct aagtgttgtt ttctttttca tttatctgtc tctatttcta attttcctac
     7321 agtttacccg actcgtagca caatcgatcg ttttaaacgt atttttcctc actcaccgcg
     7381 cgtgaatgct gatttatcgt catcttcatg gcaaactagc cgccgaaatt attgcgatca
     7441 tgccctggag gaatacgtgg ataaaatttt cgttgatgaa gcagtaaatg agctgcaaac
     7501 cattcaggac atgttgcgct ggtcggtaag ccgcttcagc gcggcaaata tctggtacgg
     7561 tcacggcacc gataacccgt gggatgaagc cgtacagctg gtgttaccat cgctctacct
     7621 gccgctggat atcccggaag atatgcgcac cgcgcgtctg acctccagcg aaaaacaccg
     7681 tattgttgaa cgcgtgatcc gccgcgtcaa cgagcgcatt ccggtggctt acctgaccaa
     7741 caaagcctgg ttctgcggtc atgaatttta cgtcgatgaa cgcgtgctgg tgccgcgctc
     7801 gccgattggt gaactgatca acaataaatt tgccggactc attagcaagc aaccgcagca
     7861 tattttagat atgtgtaccg gtagcggctg catcgccatt gcctgtgctt atgccttccc
     7921 ggaagcggaa gtcgacgcgg tagatatctc tcccgacgcg ctggcggttg ccgaacagaa
     7981 catcgaagaa cacggtctga tccacaacgt cattccgatt cgttccgatc tgttccgtga
     8041 tttgccgaaa gtgcagtacg atctgattgt cactaacccg ccgtatgtcg acgcggaaga
     8101 tatgtccgac ctgccaaacg aataccgtca cgagccggaa ctgggcctgg catctggcac
     8161 cgacggcctt aaactgacgc gtcgcattct tggaaacgcg gcagattacc ttgctgatga
     8221 tggcgtgttg atttgtgaag tcggcaacag catggtacat cttatggaac aatatccgga
     8281 tgttccgttc acctggctgg agtttgataa cggcggcgat ggtgtgttta tgctcaccaa
     8341 agagcagctg ctggcggccc gtgaatattt cgcgatttat aaagattaag taaacacgca
     8401 aacacaacaa taacggagcc gtgatggctg gaaacacaat tggacaactc tttcgcgtaa
     8461 ccaccttcgg cgaatcgcat gggctggcgc tcggctgcat cgtcgatggt gttccgccag
     8521 gcattccgct gacggaagcg gacctgcaac atgacctcga ccgtcgtcgc cctgggacat
     8581 cgcgctatac cacccagcgc cgcgagccgg atcaggtcaa aattctctcc ggtgtttttg
     8641 aaggcgttac tactggcacc agcattggct tgttgatcga aaacactgac cagcgctctc
     8701 aggattacag cgcaattaaa gacgttttcc gcccaggcca tgctgattac acctacgaac
     8761 aaaaatacgg tctgcgcgat tatcgcggcg gtggacgttc ttccgcccgc gaaaccgcca
     8821 tgcgcgtggc ggcaggggcg attgccaaaa aatatctcgc cgagaaattt ggtattgaaa
     8881 tccgcggctg cttgacccag atgggcgaca ttccgctgga aatcaaagac tggtcgctgg
     8941 ttgaacaaaa tccgttcttc tgcccggacc cggacaaaat cgacgcgtta gatgaactga
     9001 tgcgcgcgct gaaaaaagag ggcgactcca tcggcgcgaa agtcaccgtt gttgccagtg
     9061 gtgtccccgc cggacttggc gagccggtct ttgaccgcct ggatgccgac atcgcccatg
     9121 cgctgatgag catcaacgcg gtgaaaggcg tggaaattgg cgacggtttt gacgtagtgg
     9181 cgctgcgcgg cagccagaac cgcgacgaaa tcaccaaaga cggtttccag agcaaccatg
     9241 cgggcggcat tctcggcggt atcagcagcg ggcagcaaat cattgctcat atggcgctga
     9301 aaccgacctc cagcattacc gtgccggggc gcactattaa ccgctttggc gaagaagttg
     9361 agatgatcac caaaggccgt cacgatccct gtgtcgggat ccgtgcagtg ccgatcgctg
     9421 aagcgatgct ggcgatcgtt ttaatggatc acctgttacg gcagcgggcg caaaatgccg
     9481 atgtgaagac tgatattcca cgctggtaaa aaatgaacaa aaccgcgatt gcgctgctgg
     9541 ctctgcttgc cagtagcgct agcctggcag cgacgccgtg gcaaaaaata acccaacctg
     9601 tgccgggtag cgcacaatcg ataggcagtt tttctaatgg ctgtattgtc ggcgctgaca
     9661 cgttgccgat acagtccgaa cattatcagg tcatgcgtac cgatcagcgt cgctatttcg
     9721 gtcacccgga tctggtgatg tttatccagc gtctgagtag ccaggtgagc aatctgggca
     9781 tgggtacggt gctgattggc gatatgggga tgcccgctgg tgggcgtttc aacggcggtc
     9841 atgccagcca ccagaccgga ctggatgtcg atatctttct gcaactgccg aaaactcgct
     9901 ggacctccgc gcagctcttg cgcccgcaag cactggactt agtttcccgc gacggtaaac
     9961 acgttgtctc cacgctgtgg aagccagaaa ttttcagctt gatcaaactc gctgcccagg
    10021 acaaagacgt cacgcgcatt tttgttaatc cggcgattaa acaacaactt tgccttgatg
    10081 cgggcaccga tcgcgactgg ttgcgcaaag tgcgaccctg gttccagcat cgcgcgcata
    10141 tgcatgtacg attacgttgc cctgccgata gtctggagtg tgaagatcaa cctttaccgc
    10201 catcaggcga tggttgcggg gcagaactgc aaagctggtt tgcacctcta aaaccgggaa
    10261 caacaaagcc tgagaagaag acaccgcctc cgttgccgcc ttcctgccag gcgctactgg
    10321 atgagcacgt gatctaatgg aaacgtttaa tagcctgttt atggtttccc cgctgttgct
    10381 gggagttctc ttttttgtcg ccatgctggc gggatttatc gactcgattg ccggtggcgg
    10441 tgggttactc accattccgg cattgatggc tgcgggaatg tctcccgcta acgcgctggc
    10501 aaccaataaa ctgcaagcct gcggcggctc tatttccgcc actatctact ttattcgccg
    10561 caaagtggtt agtttaagcg atcagaaact caatattgcc atgacctttg tcggctcaat
    10621 gagcggcgca ctgttggtac agtacgttca ggctgatgtc ttacggcaga ttttgcccat
    10681 tctggtgatt tgtatcggcc tctatttctt gctgatgcca aagctgggtg aagaagatcg
    10741 ccagcgccgg atgtacggtt tacccttcgc gttaattgcc ggtggctgcg tcggttttta
    10801 tgacggattc tttggcccgg ctgccggatc gttttacgcg ctggccttcg ttacgctgtg
    10861 cggattcaac ctcgccaaag ccacggctca cgccaaatta ctcaacgcaa cgtcaaacat
    10921 tggcggtctg ctgctgttta ttctcggcgg caaagtgatt tgggcgacgg gctttgtcat
    10981 gctggtgggg cagttcctcg gcgcgcgcat gggttcacgg ctggtgttga gcaaaggaca
    11041 aaagctgatc cgcccgatga ttgttatcgt ctcggcggtg atgagtgcca aattacttta
    11101 cgatagccac ggacaggaga tcctccactg gttggggatg aactaatgaa cagtacacac
    11161 cactatgagc agttgattga aatttttaat agctgctttg ccgatgaatt taatacccgt
    11221 ctgattaaag gcgacgacga accgatctat cttcctgctg atgcggaagt accgtataac
    11281 cgaattgtct ttgcccacgg cttttatgcc agcgcaattc atgagatttc gcactggtgt
    11341 attgccggaa aagcgcgccg tgagctggtt gatttcggct actggtattg cccggatggg
    11401 cgtgatgccc aaacgcaaag ccagtttgaa gatgttgaag tgaagccgca ggcactcgac
    11461 tggttgttct gtgtggcggc gggatatccg tttaacgtca gttgcgacaa tctggaaggc
    11521 gacttcgaac cggatcgtgt tgtgtttcaa cggcgggtac atgcgcaggt gatggattat
    11581 ctgacaaatg gcattcccga gcgtccggca cgcttcatta aagcactaca aaattattat
    11641 cacacgccgg aacttacggc ggaacagttc ccgtggccgg aagcgctcaa ctgaagccgg
    11701 tgccacgttt tacaatgagg aaagaagatg atcgcggagt ttgaatcacg cattctggca
    11761 ttaatcgacg gcatggttga ccatgccagt gatgatgagt tgtttgccag tgggtatttg
    11821 cgtggccacc tgacattagc catcgcagaa ctggaaagtg gtgatgacca ctccgctcag
    11881 gcggtgcata cgaccgttag ccagagtctg gaaaaagcca ttggtgccgg tgaattgtcg
    11941 ccgcgtgacc aggcgctggt gaccgatatg tgggaaaacc tgtttcagca ggcgtcacag
    12001 cagtaagttg cggcgtctgg agcaccatgc ctgatgcgac gcttatcgcg tcttatcagg
    12061 cctacacgtc acacgtaggt cggataaggc gttcacgccg catccggcaa acactgcgag
    12121 agcagattac cccgccttaa ccgctttacc cttcaacaat ttccgcaccc ataaccgatt
    12181 cgggtttaac gccgtcagcg tactggcatc catcggaatc ggttcgtcgc tcatctgcgc
    12241 cgccagaatc tcggcacaca gcggggcaga acacaaaccg cgagaaccta aagccgcaaa
    12301 cataaagaga tcgtcaaaaa ccggcgcgct taccgcctca tctttctgct ccgccaacga
    12361 cgcatattcc acgagtgttg cctcataatc gggaacattg cctaccattg gcagatgatc
    12421 gcgggtggca caacgcacac cgcagcgcgc ctctttatca ctgacatcaa cctcttttgc
    12481 ccactgtgcc tgcgggaaac aatcaatcaa ccgctggcga ttctgctgct gatcgtcctc
    12541 actgtacgcc gtatcttcgc tgccgcgatg ataactggca ccaatacaat gatgttgatt
    12601 cgccggattt tgtggcgtga gataaccgtc atagcacagc acctgcttca gctctgccag
    12661 ttccggcgtt gtcggaatat ggctgacctg cccggcaacc gaatacaccg ggagagtcga
    12721 cgtttggctg aaacggctga tttgatgccc gttcgccagt accactacgc tgtgtgttgc
    12781 ttgctgatct cctgcaaaat tcaacaacca acagtcatcc ttacgggata aattctgtaa
    12841 ctgatattga taataaatct gcaaaccctg ctgttgcgcc agttccagca cattacgggt
    12901 cagttctgct gggcacagcc aaccaccttg cggataagta atgccgctgc aatttgtcgc
    12961 aacgcccgta atttgttcaa ccgcatttgc ctcaacggct acagccagtt ctgcgggtaa
    13021 atccattgac aacatctgtg cgattttatg ctggcttttc tcatcccagc ctaactgcgt
    13081 gacgccgcac cagtcatgat caaatttaac gggtaattgg tcgtaaaacc gacgagcaaa
    13141 agtaaacgca ttagagaaaa agcggtttag cgcctcatcg tgtttgctta ataacggata
    13201 cagcgccccc tggcgattgc cggaagcacc cagcgcgggg gcttcatccg cgcaataaag
    13261 cgctacctgc cagccgcgcc gtaataacgc cagcgacaac aacgcgctgg caataccacc
    13321 gccgataatc gctacttccc gtttgctgct gcccgtacga ttaaaccacg gcgtggagca
    13381 ggggagtggt aatgtctgtt ccatcacccc acaaagcatt tcccgtttgc gcccaaagcc
    13441 cttacgtttt tgcatcgtga atccggcttc ctgcaaaccg cggcggacaa aaccggcaga
    13501 cgtaaatgtc gccagcgtgc cgcctggacg cgccaacctt gccatggcgt taaacagatt
    13561 ttgcgtccac atatccgggt ttttagctgg cgcaaagccg tccagaaacc aggcatctac
    13621 tttttgattt agcgaatcgt ccagttggct ggtcagttcg ttaatatcgc caaaccataa
    13681 atccagcgtc acgcggcctt cattgagcaa taaacgatgg caaccgggca agggcattgg
    13741 ccactgcgcc tgaagttgtt ctgcccacgg agccagttcc ggccagtgtt gatgcgctaa
    13801 ggctaaatcc gcacgggtga ggggaaattt ctcaaaacta atgaaatgta agcgttgtaa
    13861 ttgcgcttgc ggatgcgctt cgcgaaactg atcaaatgcc tgccatagcg tcaggaagtt
    13921 taatccggtg ccgaagccgc tctctgctac cacaaacaga ggatgtggat gctcaggaaa
    13981 gcgtacctct aattggttgc ctcccagaaa aacataacgc gtctcttcca gcccgttatc
    14041 gttggaaaaa tagacatcgt caaaatctcg ggaaacaggt gtaccctcag cattaaattc
    14101 gaggttggca ggttgtatgg agtagtgttt cacgtaagtt actcgtctta caggcggtgg
    14161 ctcgatctta gcgatgtgtg taaggctgcg caaatttctc tattaaatgg ctgatcggac
    14221 ttgttcggcg tacaagtgta cgctattgtg cattcgaaac ttactctatg tgcgacttac
    14281 agaggtattg aatgaaacgt gcagtgatta ctggcctggg cattgtttcc agcatcggta
    14341 ataaccagca ggaagtcctg gcatctctgc gtgaaggacg ttcagggatc actttctctc
    14401 aggagctgaa ggattccggc atgcgtagcc acgtctgggg caacgtaaaa ctggatacca
    14461 ctggcctcat tgaccgcaaa gttgtgcgct ttatgagcga cgcatccatt tatgcattcc
    14521 tttctatgga gcaggcaatc gcagatgcgg gcctttctcc ggaagcttac cagaataacc
    14581 cgcgcgttgg cctgattgca ggttctggcg gcggttcccc gcgtttccag gtattcggcg
    14641 ctgacgcaat gcgcggcccg cgcggcctga aagcggttgg cccgtatgtg gtcaccaaag
    14701 cgatggcatc cggcgtttct gcctgcctcg ccacgccgtt taaaattcat ggcgttaact
    14761 actccatcag ctccgcgtgt gcgacttccg cacactgtat cggtaacgca gtagagcaga
    14821 tccaactggg caaacaggac atcgtgtttg ctggcggcgg cgaagagctg tgctgggaaa
    14881 tggcctgcga attcgacgcg atgggtgcgc tgtctactaa atacaacgac accccggaaa
    14941 aagcctcccg tacttacgac gctcaccgtg acggtttcgt tatcgctggc ggcggcggta
    15001 tggtagtggt tgaagagctg gaacacgcgc tggcgcgtgg tgctcacatc tatgctgaaa
    15061 tcgttggcta cggcgcaacc tctgatggtg cagacatggt tgctccgtct ggcgaaggcg
    15121 cagtacgttg tatgcagatg gcgatgcatg gcgttgatac cccaatcgat tacctgaact
    15181 ctcacggtac ttcgactccg gttggcgacg tgaaagagct ggcagctatc cgtgaagtgt
    15241 tcggcgataa gagcccggcg atttctgcaa ccaaagccat gaccggtcac tcactgggcg
    15301 ctgcgggtgt gcaggaagct atctactctc tgctgatgct ggaacacggc tttatcgccc
    15361 cgagcatcaa cattgaagag ctggacgagc aggctgcggg tctgaacatc gtgaccgaaa
    15421 cgaccgatcg cgaactgacc actgttatgt ctaacagctt cggcttcggc ggcaccaacg
    15481 ccacgctggt aatgcgcaag ctgaaagatt aattcgcagt aggccggagt aggtcggata
    15541 agacgcgcca gcgtcgcatc cgacgttacg cgccaatgcg gcctccggca ctaacgcaaa
    15601 agggaacctg atggttccct ttttcacatc attgacaatc gccaccagtt ccaggcaaac
    15661 ttcccgcttt gtcgatttcc ttctgaaaag acgtacgcgt taaatcctgc caacgcaccg
    15721 taaccctgaa accagagaga tgagacgggg atactcctcg ccttgcgctg cattctggag
    15781 taatgcatga ctgctgtaag ccaaaccgaa acacgatctt ctgccaattt ttcgctcttc
    15841 cgcatcgctt ttgcggtttt tctcacctac atgaccgtag ggctgccgtt gccggttatc
    15901 ccgctgtttg ttcatcatga actgggctat ggcaatacca tggtcggcat tgccgtcggg
    15961 attcagtttc tggctacggt gctgacgcgt ggctatgccg ggcgactggc cgatcaatat
    16021 ggcgcaaaac gttcggcgct tcaggggatg ttagcttgtg gtctggctgg cggcgcgttg
    16081 ctgctggcgg cgattttgcc tgtctccgca ccgttcaaat ttgccctgtt ggtcatcggg
    16141 cgtttgattc ttgggtttgg tgaaagccag ttactgacag gcgctctgac ctgggggtta
    16201 ggcatcgtag ggccaaaaca ctctggcaaa gtgatgtcat ggaatggaat ggcgatttac
    16261 ggtgccctcg ctgttggtgc tccgcttggc ctgttgattc atagccatta cggttttgcc
    16321 gcactggcga tcaccacaat ggtattaccc ttactggcgt gggcctgtaa cggcacagtg
    16381 cgcaaagtac cggccctggc gggagaacgt ccatcgctgt ggagcgttgt cgggtttatc
    16441 tggaaaccag ggttagggct ggcactacaa ggcgttggtt ttgcggttat cgggactttc
    16501 gtttcgctct actttgccag caaaggatgg gcgatggcgg gctttactct taccgagttt
    16561 ggcggcgcat ttgtcgtgat gcgcgtcatg tttggctgga tgccggaccg ttttggcggc
    16621 gtgaaagtgg cgattgtctc tctgcttgta gaaacggtgg gcttgttgct gctctggcaa
    16681 gccccaggtg cgtgggtcgc attagcgggc gcggcgttaa ccggagccgg atgttcgctt
    16741 atctttcctg cgctgggcgt ggaggtggtt aaacgcgtcc cctcacaagt tcgcggcacc
    16801 gcactgggcg gttacgccgc gtttcaggat atcgccctcg gcgtctccgg gccgctggcg
    16861 ggaatgctgg cgaccacgtt tggttactct tcggtatttc ttgccggggc gatctctgcg
    16921 gtgctgggaa ttattgtcac gatactgtca tttcgtcggg gctaacgaac cagccagacc
    16981 agcatcagga taatcgcgac taacagtatc cacagcgcgg gacgcgttgc catattttgc
    17041 aacgtgtcca tcatcggtgg aaaaggatta agcagcggtt gcatatcacg cggatcaatc
    17101 cctttaaccc gccgttgata aagctgatta agaatatcct cggcctgggc agaagaaagc
    17161 gatgattgcg cagaaagacc atatttttgc tggatatacg ccgataacgc tgccagttca
    17221 ctggcatcta aaggttgttt tagcgccatc tgtagtgatt ccagcgtcgg cgtattttgc
    17281 tggcttaacg tctggcgcgc ctgcaaccag gtcaccagat ggttaaacag tttcgctgga
    17341 attaactcgc catcgttcac cccggaaagt tccagcatcg attgccagat ctgtttgctg
    17401 ggttcccccg ttgccgccgc aagtttggtc accagctgtt ttagcgcatt gtgctccgcc
    17461 ggtaataaag gacggtcggt cgcctcgcgc tgctgtggtt gcggaataac catcttccct
    17521 tcctgcaaca gggtgagaat ggtttttaat tgctccggtg agagctgatt cagcggcgtc
    17581 tgaccaaagt tatgacggat ataatccgtg accgcctgac gattattccc cagacgtaaa
    17641 tactccccta attgcgctaa aagctggcgg gcagaatggc ttttttgcgc ggccagcaga
    17701 cgttgcgcca gattatgctc agcggcaggg aagtgacgcg aaagcagcgg tgaatctccc
    17761 gacagaccaa tatcgtgcct gatacccgcc cacagttctg ctctttgttg ttgcgtcagt
    17821 gaggtcactt tcgtcattaa gctttccagc gaagtacgtt gctgactgga taaaggctga
    17881 ttgcccgcgc cagacggcag gttatctccc tgacctggtg gttgcccagg aggagggccg
    17941 gaaataggtt gtatcattac gtatccttat acctgaaatc ttcgcaagta tgcctggccg
    18001 cgagattatg gcacacttgt ccggttaact ctcgtctcat acaggtaaca caaacgtgaa
    18061 aatccttgtt gatgaaaata tgccttatgc ccgcgactta tttagccgtt tgggtgaggt
    18121 gaccgcggtt cccgggcgtc caatccccgt cgctcaactg gcagacgcgg atgcgctgat
    18181 ggtgcgttcg gtcacgaaag tgaatgaatc tttgctggca ggaaaaccca ttaaatttgt
    18241 tggcactgcc actgcgggga ccgaccatgt cgatgaagca tggttgaagc aggcgggaat
    18301 tggtttttcc gctgcacctg gctgtaatgc gattgcggtg gtggaatatg ttttctcctc
    18361 actactgatg cttgccgaac gcgatggatt ttcactgcac gaccgtacgg tggggatcgt
    18421 gggcgttggt aacgttggac gtcgattgca ggcgcgactg gaagcgttag ggattaaaac
    18481 attgctttgc gatccgcctc gcgctgaccg tggggatgag ggtgatttcc gctcgctgga
    18541 tgaattagtt cagcatgcgg atattctgac tttccatacg ccactcttca aagatgggcc
    18601 gtacaaaacg ctgcatctgg cggatgaaaa actgatccgt agcctgaagc cgggagcgat
    18661 tctgattaac gcctgccgtg gcgcagtcgt cgataatact gcgttgctga cctgcctgaa
    18721 tgaaggccag aagttaagcg tagtgctgga tgtctgggaa ggcgaaccgg aacttaacgt
    18781 ggagttgctg acaaaagtgg atatcggcac gccgcatatc gcaggttata ccctggaagg
    18841 taaagcacgc ggtactacgc aagtgtttga agcctatagc aagtttattg ggcatgaaca
    18901 gcacgttgcg ctggatacat tactgcccgc gccagagttt ggtcgcatta cgctgcatgg
    18961 cccgctcgat caaccgacgc tgaaaaggct ggtgcatttg gtgtatgatg tgcgccgcga
    19021 tgacgcaccg ctgcgtaaag tcgccgggat accgggtgag ttcgataaac tgcgcaaaaa
    19081 ctatcttgag cgccgtgaat ggtcatctct gtatgtaatt tgtgatgacg ccagtgcggc
    19141 atcattgctg tgtaaactgg gttttaacgc cgttcatcat ccggcacgtt aatctcttct
    19201 tcatgctctc tgctgtaaca ttggcaggga gctttgctat ttctggagta aaccaccatg
    19261 tctgaaggct ggaacattgc cgtcctgggc gcaactggcg ctgtgggcga agccctgctt
    19321 gaaacgctgg ctgaacgtca gttcccggtt ggggaaattt atgcactggc acgtaacgaa
    19381 agcgcaggcg aacaactgcg ctttggtggt aaaacaatca ctgtgcagga tgccgctgaa
    19441 ttcgactgga cgcaggcgca gctggcattt tttgtcgcag gcaaagaagc taccgctgcc
    19501 tgggttgaag aagcgaccaa ctcaggttgc ctggtgatcg acagcagcgg actgtttgct
    19561 ctcgaacccg acgtaccgct ggtggtgccg gaagtaaacc cgtttgtact gaccgattac
    19621 cgcaaccgga atgtcatcgc cgtaccagac agtctgacca gccagctgct ggcggcactg
    19681 aaaccgttaa tcgatcaggg cggtttgtcg cgtatcagtg ttaccagcct gatttcagcc
    19741 tccgcccagg gcaaaaaagc ggtcgatgcg ttagcggggc agagtgcgaa attgctcaac
    19801 ggcattccga ttgacgaaga agatttcttc ggtcgtcaac tggcgttcaa tatgctgccg
    19861 ttgttgccgg atagcgaagg tagcgtgcgt gaagaacgtc gtatcgttga cgaagtacgc
    19921 aaaatcctgc aggacgaagg gctgatgatt tcggcaagcg tcgtccaggc accggtattc
    19981 tacggtcatg cccagatggt caactttgaa gcactgcgtc cgctggcggc agaagaagcg
    20041 cgtgatgcgt ttgctcaggg cgaagatatt gtgctctctg aagagaacga attcccgact
    20101 caggtagggg atgcttcggg tacgccgcat ctttctgttg gctgcgtgcg taatgactac
    20161 ggtatgccgg agcaagtcca gttctggtcg gtggccgata acgttcgctt tggcggcgcg
    20221 ctgatggcag taaaaatcgc cgagaaactg gtgcaggagt atctgtacta atgtccgacc
    20281 agcaacaact gccagtttat aaaattgcgc tgggcattga gtacgacggc agtaagtatt
    20341 acggctggca acggcagaat gaagtccgca gtgtgcagga gaagctggaa aaggcgctct
    20401 cccaggtggc gaacgaaccc atcaccgtct tctgcgccgg gcgtactgac gcaggggtac
    20461 acggtaccgg gcaggtggtg catttcgaaa caaccgcgca gcgcaaagac gcggcgtgga
    20521 ccttgggcgt aaatgcgaat ttacctggtg acatcgccgt gcgttgggtt aaagctgtac
    20581 ctgatgattt tcatgcccga tttagcgcca cggctcgccg ttatcgctac atcatctaca
    20641 atcatcggct gcgcccggcg gtattgagta aaggggtaac ccatttttac gaaccgctgg
    20701 acgctgaacg gatgcatcgg gctgcgcaat gcttgctggg cgagaatgat ttcacctcgt
    20761 tccgtgcggt gcagtgccag tcccgaaccc cgtggcgcaa cgttatgcat attaatgtca
    20821 cgcgtcacgg tccttatgtg gtggtagata tcaaagcgaa tgcctttgta catcatatgg
    20881 tcaggaatat tgtcggcagc ctgatggaag tcggtgccca caaccagccg gagagatgga
    20941 tagcagagtt gctggcggca aaggacagaa cgctggcggc agcaacggca aaagcggaag
    21001 ggctgtatct ggtcgcggtg gattaccctg accggtatga tcttccaaaa ccgccaatgg
    21061 gcccgctatt tctggcggac taacgagaat aatgctcgga acatttcagg ctgtagccaa
    21121 cgtagttaca gcctgaaaga tgacgagtac aaggcatagg caaatatgga cctgatttat
    21181 ttcctcatcg attttatcct gcacattgac gtgcatctgg cagagctggt cgcggagtac
    21241 ggcgtctggg tttatgccat tctgtttttg attctgttct gcgaaacggg gctggtggta
    21301 acgccattcc tgcctggtga ttcgctgctg tttgttgctg gtgcattggc gtcgctggaa
    21361 actaacgatc tcaatgtcca tatgatggtg gtgcttatgt tgattgccgc tattgtcggt
    21421 gatgcagtga actataccat tggacggtta tttggtgaca aactgttcag taacccggat
    21481 tcgaaaatct ttcgtcgcag ttatcttgat aagactcatc agttttacga gagacatggc
    21541 ggcaaaacga ttattctcgc ccgttttgta ccgatcgtca gaacgttcgc gccgtttgtt
    21601 gcgggaatgg gccacatgtc gtaccgtcat ttcgccgctt ataacgtgat cggcgcactg
    21661 ttgtgggtac tgatttttac ctacgcgggt tatttcttcg gcacgctccc cttcattcag
    21721 agcaacctta agctgatgat tgttggcatt atttttgttt caatattgcc tggggttttc
    21781 gaatttattc gtcacaaacg cgctgcggca cgcgccgcaa aataaaaagt aactccgcgg
    21841 ttcgaccact tttttatcca aagtttcggg ctgttatgtt ttaatgtgca acattcatgg
    21901 tctgttgggg gcaaaaatgg cattatgcgt ccccaaagat aaaactggca tcgaaccagg
    21961 ttcagacaga aaggtcccta atgagctgga ttgaacgaat taaaagcaac attactccca
    22021 cccgcaaggc gagcattcct gaaggggtgt ggactaagtg tgatagctgc ggtcaggttt
    22081 tataccgcgc tgagctggaa cgtaatcttg aggtctgtcc gaagtgtgac catcacatgc
    22141 gtatgacagc gcgtaatcgc ctgcatagcc tgttagatga aggaagcctt gtggagctgg
    22201 gtagcgagct tgagccgaaa gatgtgctga agtttcgtga ctccaagaag tataaagacc
    22261 gtctggcttc tgcgcagaaa gaaaccggcg aaaaagatgc gctggtggtg atgaaaggca
    22321 ctctgtatgg aatgccggtt gtcgctgcgg cattcgagtt cgcctttatg ggcggttcaa
    22381 tggggtctgt tgtgggtgca cgtttcgtgc gtgccgttga gcaggcgctg gaagataact
    22441 gcccgctgat ctgcttctcc gcctctggtg gcgcacgtat gcaggaagca ctgatgtcgc
    22501 tgatgcagat ggcgaaaacc tctgcggcac tggcaaaaat gcaggagcgc ggcttgccgt
    22561 acatctccgt gctgaccgac ccgacgatgg gcggtgtttc tgcaagtttc gccatgctgg
    22621 gcgatctcaa catcgctgaa ccgaaagcgt taattggctt tgccggtccg cgtgttatcg
    22681 aacagaccgt tcgcgaaaaa ctgccgccag gattccagcg cagtgaattc ctgatcgaga
    22741 aaggcgccat cgacatgatc gtccatcgtc cggaaatgcg cctgaaactg gcgagcattc
    22801 tggcgaagtt gatgaatctg ccagcgccga atcctgaagc gccgcgtgaa ggcgtagtgg
    22861 tacccccggt accggatcag gaacctgagg cctgataact gataagggca gggccactgg
    22921 ctctgccctt ttgctattct caccgtaacg aatcagcgga taccatgatt atcaaacgca
    22981 ctcctcaagc cgcgtcgcct ctggcttcgt ggctttctta tctggaaaac ctgcacagta
    23041 aaactatcga tctcggcctt gagcgcgtga gccaggtcgc ggcgcgtctt ggcgtcctga
    23101 aaccagcgcc atttgtgttt accgttgcgg gtacgaatgg caaaggcacc acctgccgta
    23161 cgctggagtc gattctgatg gcggcagggt acaaagtggg cgtctacagt tcaccgcatc
    23221 tggtgcgtta taccgagcgc gtacgtgtgc aggggcagga attgccggaa tcggcccaca
    23281 ccgcctcttt tgcggagatt gaatcggcac gcggtgatat ttccctgacc tatttcgagt
    23341 acggtacgct gtcggcgttg tggctgttca aacaggcaca acttgacgta gtgattctgg
    23401 aagtagggct gggcggtcgt ctggacgcaa ccaatattgt cgatgccgat gtggctgtag
    23461 taaccagcat tgcgctggat cataccgact ggctgggacc agatcgcgaa agtattggtc
    23521 gcgagaaagc aggcatcttt cgcagcgcaa aaccggcaat tgtcggtgag ccggaaatgc
    23581 cttctaccat tgctgatgtg gcgcaggaaa aaggtgcact gttacaacgt cggggcgttg
    23641 agtggaacta ttccgtcacc gatcatgact gggcgtttag cgatgctcac ggcacgctgg
    23701 aaaatctgcc gttgccgctt gtcccgcaac cgaatgccgc aacagcgctg gcggcactgc
    23761 gtgccagcgg gctggaagtc agtgaaaatg ccattcgcga cgggattgcc agcgcaattt
    23821 tgccgggacg tttccagatt gtgagcgagt cgccacgcgt tatttttgat gtcgcgcata
    23881 atccacatgc ggcggaatat ctcaccgggc gtatgaaagc gctaccgaaa aacgggcgcg
    23941 tgctggcggt tatcggtatg ctacatgata aagatattgc cggaactctg gcctggttga
    24001 aaagcgtggt tgatgactgg tattgtgcgc cactggaagg gccgcgcggt gccacggcag
    24061 aacaactgct tgagcatttg ggtaacggca aatcatttga tagcgttgcg caggcatggg
    24121 atgccgcaat ggcggacgct aaagcggaag acaccgtgct ggtgtgtggt tctttccaca
    24181 cggtcgcaca tgtcatggaa gtgattgacg cgaggagaag cggtggcaag taagtttcag
    24241 aatcggttag tgggcacgat cgtgctggtg gcgctggggg tgattgtact tccagggctg
    24301 ctggacgggc agaaaaaaca ttatcaggat gagtttgcgg ctatcccgct ggttccaaaa
    24361 gcgggcgatc gcgatgagcc tgatatgatg ccagccgcca cccaggcgtt gccgacgcag
    24421 ccgccggaag gcgcagcgga agaggtgcgg gcaggtgatg ccgcagcacc gtcgctcgat
    24481 ccggccacta ttgcagccaa taacaccgag tttgaaccgg aacctgcacc ggtcgcccca
    24541 ccgaagccga aaccggtgga gccgcctaaa ccgaaggttg aagcaccacc tgcgccgaag
    24601 ccagaaccga agccggtcgt ggaagaaaaa gcagcgccaa cgggtaaagc ctatgttgtg
    24661 caactgggtg cgctgaaaaa tgccgataaa gtgaatgaga ttgtcggtaa gctgcgcggt
    24721 gccggttatc gggtttatac gtcgccatcc acgccagtgc agggtaaaat tacccgtatt
    24781 ctggttgggc cggatgcctc gaaagataag ctgaaaggtt cgctgggtga gttgaagcaa
    24841 ctttctggct taagtggcgt ggtaatgggc tatacgccga attaatacag tcttgcctga
    24901 tgcgacgctg gcgcgtctta tcaggcctgg gcagtgttag aaccgtaggt cggataaggc
    24961 gtttacgccg catccgacac gcattgcacg atgccgcaaa ggcataaaaa gtcgatggcg
    25021 ttgaatattt tttcagcgcc atttttattg atgcgtggga aggaaatccc tacgcaaacg
    25081 ttttcttttt ctgttagaat gcgccccgaa caggatgaca gggcgtaaaa tcgtgggaca
    25141 catatggtct ggattgatta cgccataatc gcggtgattg ctttttcctc tctggttagc
    25201 ctgatccgcg gctttgttcg tgaagcgtta tcgctggtga catggggttg tgctttcttt
    25261 gttgccagtc attactacac ttacctgtca gtctggttta cgggctttga agacgaactg
    25321 gttcgaaatg gaattgccat cgcggtactg tttatcgcta ccctgattgt tggtgctatc
    25381 gtgaacttcg tgataggcca gttggtggag aaaacggggt tgtcaggcac cgatcgggtg
    25441 ctgggcgtct gtttcggtgc gttgcgcggt gtgttgattg ttgccgccat tctcttcttt
    25501 ctcgactcct ttaccggggt gtcgaaaagc gaagactgga gcaaatcaca gctgatcccg
    25561 cagttcagtt ttatcatcag atggtttttt gattatctgc aaagctcgtc aagtttcttg
    25621 cccagagcgt aagtgctctg agatgtggct taacgaggaa aaagacgtat gtgcggtatt
    25681 gtcggtatcg ccggtgttat gccggttaac cagtcgattt atgatgcctt aacggtgctt
    25741 cagcatcgcg gtcaggatgc cgccggcatc atcaccatag atgccaataa ctgcttccgt
    25801 ttgcgtaaag cgaacgggct ggtgagcgat gtatttgaag ctcgccatat gcagcgtttg
    25861 cagggcaata tgggcattgg tcatgtgcgt taccctacgg ctggcagctc cagcgcctct
    25921 gaagcgcagc cgttttacgt taactccccg tatggcatta cgcttgccca caacggcaat
    25981 ctgaccaacg ctcacgagtt gcgtaaaaaa ctgtttgaag aaaaacgccg ccacatcaac
    26041 accacttccg actcggaaat tctgcttaat atcttcgcta gcgagctgga caacttccgc
    26101 cactacccgc tggaagccga caatattttc gctgccattg ctgccacaaa ccgcttaatc
    26161 cgcggcgcat atgcctgtgt ggcgatgatt atcggccacg gtatggttgc tttccgcgat
    26221 ccaaacggga ttcgtccgct ggtactggga aaacgtgata ttgacgagaa ccgtacagaa
    26281 tatatggtcg cttccgaaag cgtagcgctc gatacgctgg gctttgattt cctgcgtgac
    26341 gtcgcgccgg gcgaagcgat ttacatcact gaagaagggc agttgtttac ccgtcaatgt
    26401 gctgacaatc cggtcagcaa tccgtgcctg tttgagtatg tatactttgc tcgcccggac
    26461 tcgttcatcg acaaaatttc cgtttacagc gcgcgtgtga atatgggcac gaaactgggc
    26521 gagaaaattg cccgcgaatg ggaagatctg gatatcgacg tggtgatccc gatcccagaa
    26581 acctcgtgtg atatcgcgct ggaaattgct cgtattctgg gcaaaccgta ccgccagggc
    26641 ttcgttaaaa accgctatgt tggccgcacc tttatcatgc cgggccagca gctgcgtcgt
    26701 aagtccgtgc gccgtaaact gaatgccaac cgcgccgagt tccgcgataa aaacgtcctg
    26761 ctggtcgacg actccatcgt ccgtggcacc acttctgagc agattatcga gatggcacgc
    26821 gaagccggag cgaagaaagt gtacctcgct tctgcggcac cggaaattcg cttcccgaac
    26881 gtttatggta ttgatatgcc gagcgccacg gaactgatcg ctcacggtcg cgaagttgat
    26941 gaaattcgcc agatcatcgg tgctgacggg ttgattttcc aggatctgaa cgatctgatc
    27001 gacgccgttc gcgctgaaaa tccggatatc cagcagtttg aatgctcggt gttcaacggc
    27061 gtctacgtca ccaaagatgt tgatcagggc tacctcgatt tcctcgatac gttacgtaat
    27121 gatgacgcca aagcagtgca acgtcagaac gaagtggaaa atctcgaaat gcataacgaa
    27181 ggatgatgcc ttcgctgagg gtgccggtct ggcaccctga cttgcaactc ccgccgaaat
    27241 actgcaaagt ctgcctgcaa gtctgacagg gcaactattt atgaaacgac tcattgtagg
    27301 catcagcggt gccagcggcg cgatttatgg cgtgcgctta ttacaggttc tgcgcgatgt
    27361 cacagatatc gaaacgcatc tggtgatgag ccaggcggcg cgccagacct tatccctcga
    27421 aacggatttt tccctgcgcg aagtgcaggc attagctgat gtcacgcacg atgcgcgcga
    27481 tatttccgcc agcatctctt ccggttcttt ccagacgctg gggatggtga ttttaccctg
    27541 ttcaatcaaa accctttccg gcattgtcca tagctatact gatggcttac tgacccgtgc
    27601 ggcagatgtg gtgctgaaag agcgtcgccc gttggtgctc tgcgtgcgtg aaacaccatt
    27661 gcacttaggc catctgcgtt taatgactca ggcggcagaa atcggtgcgg tgattatgcc
    27721 tcccgttccg gcgttttatc atcgcccgca atcccttgat gatgtgataa atcagacggt
    27781 taatcgtgtt cttgaccagt ttgcaataac ccttcctgaa gatctctttg cccgctggca
    27841 gggcgcataa ctccgctgtt gccctgtttc agggcaattt tgcaaccgcg atcaaatcct
    27901 cgacattttg tttctgccat tcaatcgaaa cgctgcgatt caaccgctat acctgctatc
    27961 ttcaacttca ggacaataat gcaacgtctt attaacatat ttaacgttga atgttactgt
    28021 tgtcgtcaag atggcataag acctgcatga aagagcctgc aaacacacaa cacaatacac
    28081 aacataaaaa agccattttc acttgagggt tatgtatgaa gaagtcgatt ctcgctctgt
    28141 ctttgttagt cggtctctcc gcagcggctt ccagctatgc ggcgctaccg gagacggtac
    28201 gtatcggaac cgataccacc tatgcgccgt tctcgtcgaa agatgctaaa ggtgattttg
    28261 ttggctttga tatcgatctc ggtaacgaga tgtgcaaacg gatgcaggtg aaatgtacct
    28321 gggttgccag tgactttgac gcgctgatcc cctcactgaa agcgaaaaaa atcgacgcca
    28381 ttatttcgtc gctttccatt accgataaac gtcagcagga gattgccttc tccgacaagc
    28441 tgtacgccgc agattctcgt ttgattgctg ccaaaggttc accgattcag ccaacgctgg
    28501 attcactgaa aggtaaacat gttggtgtgc tgcagggatc aacccaggaa gcttacgcta
    28561 acgagacctg gcgtagcaaa ggcgtggatg tggtggccta tgccaaccag gatttggtct
    28621 attccgatct ggctgcagga cgtctggatg ctgcgttaca agatgaagtt gctgccagcg
    28681 aaggattcct caagcaacct gctggtaaag ctttcgcctt tgctggctca tcagtaaaag
    28741 acaaaaaata cttcggtgat ggcaccggtg tagggctacg taaagatgat gctgaactga
    28801 cggctgcctt caataaggcg cttggcgagc tgcgtcagga cggcacctac gacaagatgg
    28861 cgaaaaagta tttcgacttt aatgtctacg gtgactgata cgtcgctggg aagctgtacc
    28921 tgatggaatg atcatcatgg tgcacgccag gtttgttgca ctatcgtggt gcattgaaat
    28981 gcatacttaa gcatttttaa tgaaaaataa tacgtctaac ggggcggaat attttgcctt
    29041 gatggtcaat tttatggcac gataagtgta acaaacctgt aaatattccc tataaaaaga
    29101 ctgtcagttg aggacattat gaaaaaactg gtgctatcgc tctctctggt tctggccttc
    29161 tccagcgcaa ctgcggcatt tgctgcgatt ccgcaaaaca tccgcatcgg taccgacccg
    29221 acctatgcgc catttgaatc aaaaaattca caaggcgaac tggttggctt cgatatcgat
    29281 ctggcaaagg aattatgcaa acgcatcaat acgcaatgta cgtttgtcga aaatccgctg
    29341 gatgcgttaa tcccgtcctt aaaagcgaag aagattgacg ccatcatgtc atcgctttcc
    29401 attacggaaa aacgtcagca agaaatagcc ttcaccgaca aactgtacgc tgccgattct
    29461 cgtttggtgg tggcgaaaaa ttctgacatt cagccgacag tcgagtcgct gaaaggcaaa
    29521 cgggtaggcg tattgcaggg caccacccag gagacgttcg gtaatgaaca ttgggcacca
    29581 aaaggcattg aaatcgtctc gtatcagggg caggacaaca tttattctga cctgactgcc
    29641 ggacgtattg atgccgcgtt ccaggatgag gtcgctgcca gcgaaggttt cctcaaacaa
    29701 cctgtcggta aagattacaa attcggtggc ccgtctgtta aagatgaaaa actgtttggc
    29761 gtagggaccg gcatgggcct gcgtaaagaa gataacgaac tgcgcgaagc actgaacaaa
    29821 gcctttgccg aaatgcgcgc tgacggtact tacgagaaat tagcgaaaaa gtacttcgat
    29881 tttgatgttt atggtggcta atcgccatcg gtgaaagaaa gcccgctccc ttcgggtaac
    29941 cggagggaga caagaggcgg taattcacca cacacgacag gacaggcagc atgttgtatg
    30001 ggttttcagg tgttatttta cagggtgcgc tcgtcacgct ggagctggct atcagctctg
    30061 tagtgctcgc tgtaatcatc ggtttaattg gcgctggcgg taagctctcg caaaatcggc
    30121 tttcggggct gattttcgaa gggtacacca cgctgattcg tggcgtgccg gacttagtgt
    30181 tgatgctgct gattttctac ggtttgcaga ttgcgctaaa cacggtgacg gaggcgatgg
    30241 gcgtcgggca gattgatatc gatccgatgg tcgctggtat tatcactctc ggttttatct
    30301 acggtgctta ttttaccgaa acgttccgtg gtgcttttat ggcagtgccg aaaggacata
    30361 tagaggcggc gacggcgttc ggttttactc gtgggcaagt gtttcggcgg atcatgtttc
    30421 cggcgatgat gcgttacgcc ctgccaggca ttggcaacaa ctggcaggtg atcctcaaat
    30481 ctaccgcact ggtttcgtta ctcggcctgg aagatgtggt caaagccacc caactggcag
    30541 gcaaaagtac ctgggaaccg ttctatttcg ccatcgtctg tggcgtgatt tacctggttt
    30601 tcaccactgt ttccaatggt gtgctgctgt tccttgagcg ccgctactcc gtgggtgtga
    30661 agagggctga cctgtgatcg aaatcttaca tgaatactgg aaaccgctgc tgtggaccga
    30721 cggttatcgc tttactggtg tggcgatcac tctgtggctg cttattttgt cggtagtgat
    30781 aggcggagtc ctggcgctgt ttctggcgat tggtcgtgtc tccagtaata aatacatcca
    30841 gtttccaatc tggttattta cctatatttt tcgcggtacg ccgctgtatg ttcagttgct
    30901 ggtgttctat tccggcatgt acacgcttga gattgttaag ggaaccgaat tccttaacgc
    30961 tttcttccgc agtggcctga actgtaccgt gctggcgctg acgcttaaca cctgcgctta
    31021 cactaccgag atttttgctg gggcaatccg ttcggttccg catggggaaa ttgaagccgc
    31081 cagagcctat ggcttctcga cttttaaaat gtatcgttgc attattttgc cttctgcgct
    31141 gcgtattgcg ttaccggcat acagcaacga agtgatcctg atgctgcact ctactgcgct
    31201 ggcgtttacg gccactgtgc cggatctgct gaaaatagcc cgcgatatta acgccgccac
    31261 gtatcaacct tttaccgcct tcggcattgc cgcggtgctc tatttaatca tctcttatgt
    31321 cctgatcagc ctctttcgca gagcggaaaa acgctggttg cagcatgtga aaccttcttc
    31381 aacgcactga gaatacgatg tccgagaata aattaaacgt tatcgatttg cacaaacgct
    31441 acggcgaaca tgaagtgctg aaaggggtct cactgcaagc gaatgccgga gatgtaataa
    31501 gcatcatcgg atcgtcggga tcggggaaaa gtacctttct gcgctgcatt aacttcctcg
    31561 aaaaaccgag tgaagggtcg atcgtggtca atggccagac gatcaatctg gtgcgcgaca
    31621 aagacggtca actcaaagtc gccgataaaa atcaactgcg cttactgcgc acacgcctga
    31681 cgatggtatt ccagcacttc aatctctgga gccatatgac ggtgctggaa aacgtcatgg
    31741 aagcgccgat tcaggtgttg ggcctgagca agcaggaagc gcgcgagcgg gcggtgaagt
    31801 atctggcgaa agtcgggata gacgaacgtg cgcaggggaa atatccggtg catctttccg
    31861 gcggtcagca acagcgtgtt tctatcgcgc gggcgctggc gatggaaccg gaagttttac
    31921 tgtttgatga acctacctcg gcgctcgatc ctgaactggt aggcgaagtg ttacgtatta
    31981 tgcagcaact ggcagaagag gggaaaacca tggtggtagt gactcacgaa atgggctttg
    32041 ctcgccatgt ttctactcac gtcattttcc tccatcaggg gaaaatagaa gaagagggcg
    32101 cgccggagca gttatttggc aacccgcaaa gccctcgtct gcaacagttc cttaagggat
    32161 cgctgaaata atttcctctc cggtggctaa cgctgccgga gtattttcat ggggaatttg
    32221 cgagtcagct tccgctgaaa tgtaaaagtt gcagtccgct aaaaatactt ctgaattcgc
    32281 tgtccaaaga taaaaactgc ctgctgcatt gggtgttaat cgtggttatt gtcgcgtcat
    32341 cttaaatttg aggggtgacg gaacgccatg acaatatcga caacttccac gccgcatgat
    32401 gcggtattta aatctttttt acgccatcca ga
//
LOCUS       DAZOXR010000029        31699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZOXR010000029 DAZOXR010000000
VERSION     DAZOXR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.029"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUNN_001143"
     CDS             <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUNN_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0575582.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUNN_001144"
     CDS             1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUNN_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0575583.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1895..2752
                     /locus_tag="ACHUNN_001145"
     CDS             1895..2752
                     /locus_tag="ACHUNN_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0575584.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUNN_001146"
                     /pseudo
     CDS             complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUNN_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUNN_001147"
     CDS             complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUNN_001147"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0575585.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUNN_001148"
                     /pseudo
     CDS             complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUNN_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7412..8659)
                     /locus_tag="ACHUNN_001149"
     CDS             complement(7412..8659)
                     /locus_tag="ACHUNN_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR0575586.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8681..9007)
                     /locus_tag="ACHUNN_001150"
     CDS             complement(8681..9007)
                     /locus_tag="ACHUNN_001150"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0575587.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUNN_001151"
     CDS             complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUNN_001151"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0575588.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10396..11652
                     /locus_tag="ACHUNN_001152"
     CDS             10396..11652
                     /locus_tag="ACHUNN_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0575589.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUNN_001153"
     CDS             11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUNN_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0575590.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12140..13378)
                     /locus_tag="ACHUNN_001154"
     CDS             complement(12140..13378)
                     /locus_tag="ACHUNN_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0575591.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13714..14916
                     /gene="nupC"
                     /locus_tag="ACHUNN_001155"
     CDS             13714..14916
                     /gene="nupC"
                     /locus_tag="ACHUNN_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0575592.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14966..17154)
                     /locus_tag="ACHUNN_001156"
                     /pseudo
     CDS             complement(14966..17154)
                     /locus_tag="ACHUNN_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(17363..17438)
                     /locus_tag="ACHUNN_001157"
     tRNA            complement(17363..17438)
                     /locus_tag="ACHUNN_001157"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17403..17405),aa:Ala,seq:ggc)
     gene            complement(17478..17553)
                     /locus_tag="ACHUNN_001158"
     tRNA            complement(17478..17553)
                     /locus_tag="ACHUNN_001158"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17518..17520),aa:Ala,seq:ggc)
     gene            17774..18133
                     /locus_tag="ACHUNN_001159"
     CDS             17774..18133
                     /locus_tag="ACHUNN_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0575593.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18135..18527
                     /locus_tag="ACHUNN_001160"
     CDS             18135..18527
                     /locus_tag="ACHUNN_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0575594.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="ACHUNN_001161"
     CDS             complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="ACHUNN_001161"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0575595.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20253..20328
                     /locus_tag="ACHUNN_001162"
     tRNA            20253..20328
                     /locus_tag="ACHUNN_001162"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20286..20288,aa:Val,seq:tac)
     gene            20373..20448
                     /locus_tag="ACHUNN_001163"
     tRNA            20373..20448
                     /locus_tag="ACHUNN_001163"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20406..20408,aa:Val,seq:tac)
     gene            20495..20570
                     /locus_tag="ACHUNN_001164"
     tRNA            20495..20570
                     /locus_tag="ACHUNN_001164"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20528..20530,aa:Val,seq:tac)
     gene            20575..20650
                     /locus_tag="ACHUNN_001165"
     tRNA            20575..20650
                     /locus_tag="ACHUNN_001165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20608..20610,aa:Lys,seq:ttt)
     gene            20681..20806
                     /locus_tag="ACHUNN_001166"
     ncRNA           20681..20806
                     /ncRNA_class="other"
                     /locus_tag="ACHUNN_001166"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            20926..21267
                     /locus_tag="ACHUNN_001167"
     CDS             20926..21267
                     /locus_tag="ACHUNN_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0575596.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21258..22184)
                     /locus_tag="ACHUNN_001168"
     CDS             complement(21258..22184)
                     /locus_tag="ACHUNN_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0575597.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22274..23272
                     /locus_tag="ACHUNN_001169"
     CDS             22274..23272
                     /locus_tag="ACHUNN_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0575598.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23269..23487)
                     /locus_tag="ACHUNN_001170"
     CDS             complement(23269..23487)
                     /locus_tag="ACHUNN_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0575599.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23489..25504)
                     /gene="ligA"
                     /locus_tag="ACHUNN_001171"
     CDS             complement(23489..25504)
                     /gene="ligA"
                     /locus_tag="ACHUNN_001171"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0575600.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25575..26561)
                     /gene="zipA"
                     /locus_tag="ACHUNN_001172"
     CDS             complement(25575..26561)
                     /gene="zipA"
                     /locus_tag="ACHUNN_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0575601.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26791..27552
                     /gene="cysZ"
                     /locus_tag="ACHUNN_001173"
     CDS             26791..27552
                     /gene="cysZ"
                     /locus_tag="ACHUNN_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0575602.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27737..28708
                     /gene="cysK"
                     /locus_tag="ACHUNN_001174"
     CDS             27737..28708
                     /gene="cysK"
                     /locus_tag="ACHUNN_001174"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0575603.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29092..29349
                     /gene="ptsH"
                     /locus_tag="ACHUNN_001175"
     CDS             29092..29349
                     /gene="ptsH"
                     /locus_tag="ACHUNN_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0575604.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29394..31121
                     /gene="ptsI"
                     /locus_tag="ACHUNN_001176"
     CDS             29394..31121
                     /gene="ptsI"
                     /locus_tag="ACHUNN_001176"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0575605.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31162..31671
                     /gene="crr"
                     /locus_tag="ACHUNN_001177"
     CDS             31162..31671
                     /gene="crr"
                     /locus_tag="ACHUNN_001177"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0575606.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7821 a         8355 c         7880 g         7643 t
ORIGIN      
        1 ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag accaagctgg
       61 cgctggatat cgccaacaaa acgctaccgc tgtttcgcca tgtcaatagc gagtcattac
      121 gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga tgcggtggcg attactaata
      181 ccgatcatgt gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact
      241 tcattagccc gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg
      301 atgaagccca ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag
      361 gggtcgtgac cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat
      421 tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg
      481 ccgagcagct acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta
      541 atccccattt tctgtttaac gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg
      601 ataccgctcg ccagttgatc tttaatctgt cgcgttatct gcgctataac attgaattaa
      661 aagacgatga gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca
      721 ttgagcaggc ccgctttggt gacaagctga cggttatcta tgatattgat gaagaggtga
      781 attgctgcat tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta
      841 ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg
      901 tacgcattgc ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg
      961 aagcgaatga aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt
     1021 tattgtatgg cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt
     1081 acattcctaa ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag
     1141 tgatattgtg aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg
     1201 gctaattaaa gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt
     1261 gttgaagttt ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc
     1321 gctggatggc gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt
     1381 gttcatcacc gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta
     1441 cattctcaaa ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc
     1501 ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac
     1561 cattaatctg gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc
     1621 cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat
     1681 gaacattacc gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc
     1741 attttgtgtc aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat
     1801 tctgcgactg aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt
     1861 tcgccagtta atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga
     1921 tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg
     1981 cgtctggttt gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc
     2041 ccgtctggat atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg
     2101 aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa
     2161 caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta
     2221 cgatagccgc accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca
     2281 acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga
     2341 gcagaatatc attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa
     2401 tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg
     2461 ggttcaggat aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat
     2521 cacgcccaat catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta
     2581 tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat
     2641 tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg
     2701 gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa
     2761 ggttaattct gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga
     2821 ctggcggaag tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt
     2881 catcccttcg ttagcaccca gcgtcagcat gatcaccagt tcaacttcgc ccatttctga
     2941 ctgccagtca accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg
     3001 agatttggtg tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg
     3061 ctgccagaca tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt
     3121 gccgcacagg aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt
     3181 ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc
     3241 aatttcctgc gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct
     3301 atccagttga cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc
     3361 atccaggccc agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca
     3421 aatgcctacc catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag
     3481 gaacgatggc gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc
     3541 atacagatac tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa
     3601 gtggtcgatg atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc
     3661 tgcatgacgc aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac
     3721 ccataagatc tgatcgaggc tgttaaccat cgggatcatc aactgggcgt tgccgaaact
     3781 ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat
     3841 acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat
     3901 gcttttatcg ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag
     3961 tagcacctgc tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat
     4021 atagagcatt tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg
     4081 cgcttccaga gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata
     4141 ggccagctgc gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc
     4201 gacctgataa taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc
     4261 gtcaagcact gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag
     4321 taccgggatc gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc
     4381 cagaatcatg cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc
     4441 ttcagccacc agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg
     4501 ctaggtgata tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag
     4561 ataatcgctg gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc
     4621 cgcccccagt ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc
     4681 atcctgaatc agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg
     4741 caattgctgg ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc
     4801 ttgcgcactg gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag
     4861 cgtacccacg ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg
     4921 gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc
     4981 gctgtcgata aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc
     5041 gctgccgcta atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc
     5101 cagcgagctt ttagcatcgg cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct
     5161 ttgccactgg ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg
     5221 caaaggacag agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct
     5281 gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag
     5341 ttgctccatc tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg
     5401 ggtggcaggc cccattacca ctgtgggtac gccagtatcg gttaagtgca ccgctccacc
     5461 gtccgtgccg ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgacac
     5521 ggtttcaatc cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag
     5581 catcggcccg ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca
     5641 gcaggcggta tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc
     5701 gccgcgtaat cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc
     5761 ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa
     5821 ggctttcccc atcacccgct ggtgagggag aacctgaaaa gctgtatcaa aggtgacgcg
     5881 atcgcctgga cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac
     5941 gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca
     6001 ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc
     6061 aaccggcagc acatcaattg ccccttcgcg ggagatgctg cgcaccataa atcccacttc
     6121 gtccatatgc gcacagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac
     6181 cgatcccaga ccatcaaatc gtacttcttt ttgcaggcga tccgcttctt ccagtaggat
     6241 ctgccgcact tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag
     6301 cgataaatcc attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact
     6361 tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc
     6421 aaataaatcc ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc
     6481 ggtgaaaagc gtggatcttc gtgtacttca ataccgatag catgaccggt gttgtgacca
     6541 aaatagtcgc caaatcctgc ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag
     6601 cagcgtacgc cagggcgaat cgcggagatt gccgccagct gtgcttgcag gacaatctga
     6661 tagacgttaa acagcgggtg agattcggca attacccctt cgccattcac cagcaaggtg
     6721 cgcgtcatat cagagcagta cccctgatag agcgcaccaa aatcaagagt gacaaactcg
     6781 cctgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccgctg
     6841 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     6901 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     6961 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     7021 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     7081 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     7141 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
     7201 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     7261 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
     7321 ttctgccgtg aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc
     7381 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttacctt
     7441 tacggaacat catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg
     7501 cgatccccgc catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
     7561 ccgggaacca ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga
     7621 ccatatacga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     7681 taatccccat aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     7741 acaggcgttt gccggttaaa cgacggtcga taatggtcgc cagacccaga cctatcggcg
     7801 gaataacgat agcgatagaa cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga
     7861 aggcaacaaa acctgcggct ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg
     7921 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga
     7981 taccgccgtt gatccagccg ccaaaggggg taatgacgta gtacatcgcc agcatgacaa
     8041 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     8101 actggatttt ctggttcatc cacttcacaa ggtaacccgc gacgatagaa ataatcagcg
     8161 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     8221 agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg
     8281 ccagtttgcc accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa
     8341 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac
     8401 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     8461 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     8521 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     8581 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     8641 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     8701 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     8761 tttcataaac gtcgcgtgat tcgaaacgct cattatcttc cggggtaact gccacggagt
     8821 ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg
     8881 cgccctgggt ttcaattttc acttcataac ctgcttctac cgccgcttct tccagcgcct
     8941 gagcggccat aaaggtgtga gccaggccca ttgggcaggc acataaggca attagtttct
     9001 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
     9061 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     9121 gtgtgacgca ggtcggcctt gtgaggggga cacaaagaaa aattgaatta actgtatgat
     9181 ttaaaagatt atcgggagag ttacctcccg atataacaga aaggatttac agaatgtgcc
     9241 ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga
     9301 gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac
     9361 ggaaaccaga ggctttgaag aactcaagga agcgcggcac gataccgcca gcaatataaa
     9421 ccccaccaaa ggttcccagc gttaatgcca gattgccgcc aaaacggccc ataatgacgc
     9481 aaaacagcga caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa
     9541 tatcttttgg cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca
     9601 aattcaccag cccaggacca gaaagcacgc gttccgccga aacatgacca atttccgcac
     9661 gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc
     9721 cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc
     9781 ctgttccggc accgtaaacc gcaataggtt taccttcgac cggttctgcg ccaccaaact
     9841 gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa
     9901 taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg
     9961 tatggttggt cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt
    10021 ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt
    10081 aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag
    10141 caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac
    10201 tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac
    10261 aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacattt actttatcgt
    10321 ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg
    10381 cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc
    10441 gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg
    10501 gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca
    10561 cctatctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt
    10621 ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccagtt
    10681 ccgccctctg cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc
    10741 agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc
    10801 gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc
    10861 atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat
    10921 ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct
    10981 ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac
    11041 ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca
    11101 gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa
    11161 aatccggtgc tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt
    11221 ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct
    11281 ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct
    11341 gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta
    11401 gggttgatgc tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct
    11461 attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca
    11521 gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg
    11581 ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac
    11641 gataatgttt agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg
    11701 gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga
    11761 ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca
    11821 gcaaatgcag gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat
    11881 cattacggta attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat
    11941 tatgaatggc gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat
    12001 aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc
    12061 ggaaaaggcc gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc
    12121 tgatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca
    12181 gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca
    12241 gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga
    12301 taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct aatcccatca
    12361 gaatgacaat aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga
    12421 agcgaatgaa tccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgaggaca
    12481 gtccggcagc aaccagactt aacccaaaga ccgttgccgc agcatggctt aacagcggtt
    12541 gcagcgtcag ataagcctca tcaagatcgg caacaccagt atgaccagaa aagtggaacg
    12601 ccgccgcagc tgtagccatc atcgccagat tgacaaaacc ggcaatcgtc atggcgatag
    12661 ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt aaatgctgag
    12721 tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac acgcctgctg
    12781 ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac cattccttta cccagctgcg
    12841 ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcggcaaca aacaacagta
    12901 acccgccaat cactttctcc agtggttttt gcccgcgacg ttgcagcatt aaaatcagga
    12961 aagtcgcgat ccccgtcagc accgcgccct gcaacaacga gacaccaaga atgagtttaa
    13021 aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata atttctgcct
    13081 gaacccaata gaaccacact acgggacgcg gatagtgatc acgaatctgc tccgccagat
    13141 tttttccggt ggcaatccct agtttggcag agaggatctg aatcagcatc gccatcaggt
    13201 tggcccaaac gacaacccac agtagctgat agccgaagct ggcacccgcc tgaatattgg
    13261 tcgcaaagtt accgggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg
    13321 cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag ttcgtcatct
    13381 tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gataaacgag aatgattatc
    13441 aaattcattt aaatggattg tggtgatttc tctgatagac caggattatg actacgaaaa
    13501 gattgatgac ttgaatgtta tggttatgtt taatgttagc acatttacat aactttcagc
    13561 ttccatacac aacatagcag aaatgtatga cagatcacta tttttgaagc ctgtcacagg
    13621 acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac
    13681 ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg
    13741 gcacttgccg ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt
    13801 atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac
    13861 tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa actgctcaga
    13921 tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc
    13981 ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cactgatcgg tattctccag
    14041 cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac
    14101 ggtatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg tcagtctgaa
    14161 aactttattg cctataaaga tatcctcggc aaaatctccc gtaatcgtat gtacaccatg
    14221 gctgccacgg caatgtccac cgtgtcgatg tccatcgttg gtgcatacat gaccatgctg
    14281 gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt tatcgtgctg
    14341 tcgctgatca atccttaccg tgttgatgcc agtgaagaaa acatccagat gtccaacctg
    14401 cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg tttcaaagtt
    14461 gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc gctgaacgca
    14521 ctgtttgcca ccgttactgg ctggtttggc tacagcatct ccttccaggg catcctgggc
    14581 tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc actgcaagtg
    14641 ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag
    14701 aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctggtttcc
    14761 ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag
    14821 caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac cctggtgagt
    14881 gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa aagccgggga
    14941 tcattccccg gcttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat
    15001 acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga
    15061 caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa
    15121 tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa gacgccatca attttgatga
    15181 tatcagcctg caaacgcttt aaccgttcgt agttggcata tccggtgcca aagtcatcaa
    15241 tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt
    15301 tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac
    15361 gcttaaacag acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta
    15421 aattgactga aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc cacttcaaca
    15481 aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca
    15541 gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg atctcatcat
    15601 aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca ccctgatcta
    15661 acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct
    15721 cctctctgct atccagcgcc agcacatgat gatgtgcgca ggattgctcc gccagccagc
    15781 ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag
    15841 caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt
    15901 taaccatatg ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac
    15961 tacccggcaa ttgatataac ttttcgtttt cctgcatcaa cggcagcagc gtacggtaaa
    16021 ttgatcggat acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat
    16081 tatcaatgcg caggcagcaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    16141 acggaagttg ggcaggagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcgatt
    16201 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    16261 cagcaccgca agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    16321 gttgtaattc agaaggcaaa gcgttgaaac agccctggtg agatttaaaa acggatagcg
    16381 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    16441 aaagtcgttt tcataaggtg tgcacaacag aagtagaagc acgcttaacg ctgccagcca
    16501 gcttaaggta aatgcgcgtt tctctttgcc caacgacggc gcgatatccc tgcgccacaa
    16561 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    16621 aataagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
    16681 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    16741 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    16801 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    16861 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    16921 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    16981 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    17041 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    17101 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    17161 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    17221 gcaaacaggg ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggca
    17281 ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc
    17341 tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc
    17401 atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaaat
    17461 ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
    17521 atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg
    17581 gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa
    17641 tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc
    17701 atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat
    17761 tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg
    17821 ctatagccgc cagaccatta ataaatgggt acgcaaggag ggctggacaa cgtcaccaaa
    17881 acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata
    17941 tattcgcaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc
    18001 gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca
    18061 gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg
    18121 cgatagcaaa taatatgaaa agattacgca ataaaatgac caccgaagaa ctggctgaat
    18181 gcctcggtgt ggctaaacaa actgttaacc gttggatcag agaaaaaggc tggaaaacgg
    18241 aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt
    18301 gcgagtttat tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag
    18361 agagaattgc tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg
    18421 cgatagataa tatgacagac attgagcagg gaaaagtcgc gcaatttttg tcacgtgaag
    18481 gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca
    18541 ggatattatc tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca
    18601 gcaataaaat ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga
    18661 acggtaacat ccagtgctgg agactgcccc gccccggtta cggcgacacg cagcggcata
    18721 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
    18781 ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
    18841 cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg
    18901 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt
    18961 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
    19021 cactgtaagt gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc
    19081 cacagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag
    19141 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
    19201 accagatagt tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg
    19261 actgccccgt gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc
    19321 gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg
    19381 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
    19441 acacagaagt tataggttgg ggaaccatcg gtacggcgga taataagatc gtccagttcc
    19501 tggttgctga actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
    19561 gggttagcaa aacgtacaac acacggttca tcatcagcat gatgctcatg gctgtggcgg
    19621 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
    19681 aggcgctctt tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc
    19741 accgcgttgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
    19801 ctcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
    19861 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
    19921 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
    19981 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
    20041 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
    20101 tctaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
    20161 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
    20221 aactctccct ataatgcggc tccacacagc gggggtgatt agctcagctg ggagagcacc
    20281 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
    20341 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
    20401 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaat ttctcgccag
    20461 ctaaatttct tgtaaaaatg tgaagtaccg aagtgggtga ttagctcagc tgggagagca
    20521 cctcccttac aaggaggggg tcggcggttc gatcccgtca tcacccacca cttcgggtcg
    20581 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
    20641 cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatactcaaa
    20701 gattcgaacc tgcagcaggt ttgagttgag cgcagcgaaa caacggagcc gctcgcggcg
    20761 acggcccgaa gggcgagcga agcgagtcat cctgcacgac ccaccaatgt aaaaaagcgc
    20821 cctaaaggcg cttttttgct atctgcgatt tgtaagattg atttttcttc tcaagttcct
    20881 tgttcttctg ccgatattac ctcgtacagt gacgaggact gaacaatggc cactatctct
    20941 tcaacttcaa ttccttccat acaaacacag agtagtaaca gggcatcaca aggtagcgat
    21001 gttgcttcaa aaatcgcgcg gataagtcag caaatcatca aactcactca acaaattaaa
    21061 gagattgtag acactagcgg cagtgctgaa gacaaacaaa aacaggccga attgattcaa
    21121 cagcaaatca ccctactgga aacacaactg gcgcagttgc agagacagca ggcagaaaag
    21181 gcgcaggaga aagagcagcg tttgtctctg aatgtatccc ttctaaatcc tgttgaaaat
    21241 acgactcata tcgatatcta tatctgatac aacggagccc cttcccgacc agccattagc
    21301 gcattgccct ggtctcgtac cacgtcccat aacgcttcag cggcggtgga aagtgaacgg
    21361 ttcttcctgc gcaccagcat caactggcgt tcaacaaccg gcgtaatgcg ctttaccacc
    21421 agtgggctac cttcaggcaa tggtagcgcc agtgcgggaa gaatactgat gccgatgcct
    21481 gcggctacca tcgggaacag cgtcgccgga tgtccaatct cctgcacaat attcgcctga
    21541 ataccattgc gcgccagcgc tgcgtcaatc agcggtcggc tgcctgacgc gtaatcctgc
    21601 aacaccaatt tcgctccctg taatgcctgc cagggtacat aatcttccac ggccaaagcg
    21661 ctatcgcgat ggcaaagcag aaagaaaggc tcggaaagaa tcgcttcaca ttgcagatcg
    21721 cccactgggc ctggatcgat gacgatgcaa aaatcgacat ctccctggcg aatactttcc
    21781 atcacccact gctgcggacg atcgtgcagg acaaactgaa tatctggata gcggcgatgg
    21841 ctttcagcaa tgcattgcgg aataagatgc gcggaaatgg tctggctggc agcgacccga
    21901 acttttccgc tcagttgttg ccccatacgc ccggtatcgc gcaacgtgct gttaagttca
    21961 tccagcagtc gctcaagacg caaagccaac tgctgccctg catctgtaag caccacttca
    22021 cgcgtggttc tgtccagcag gcgaacacca gtatgatttt ccagctcctt cacactgtga
    22081 cttactgccg actggctcaa gccgatacgc tctcctgcac gactaaaact tttctcctgc
    22141 gctactgtga cgaaaacctt taattgtttt aaagaataat tcatctgttt tcttcatgaa
    22201 tggatgcaat aaatcaattt tatttctcaa actggaagaa gcacaataga accatcgatc
    22261 atctggagtc tttatgaaac tttttcgtat cctcgatcct ttcaccttaa ccctgatcac
    22321 ggtggtgttg ctggcctctt tctttccggc cagaggcgat ttcgtcccct tctttgaaaa
    22381 tctgaccacc gcagctattg ccctgctgtt ctttatgcac ggcgcgaagt tgtcgcgtga
    22441 ggcgattatt gctggcggtg gtcactggcg actgcatttg tgggtaatgt gcagcacctt
    22501 cgtgctgttt ccgattctgg gtgtactgtt tgcctggtgg aaaccggtaa atgtcgaccc
    22561 gatgctctac tccggttttc tctacttgtg cattctcccg gctaccgtgc agtctgcaat
    22621 cgccttcacg tcaatggcgg gcggtaacgt cgcggcggcg gtttgttctg cgtcagcatc
    22681 cagcctgctg gggattttcc tttcaccatt gctagttggt ctggtgatga atgttcacgg
    22741 tgcagggggc agccttgagc aggtcggtaa aattatgctg caactgctgc tgccgtttgt
    22801 gttggggcat ctttcccggc cgtggattgg tgactgggtg tcgcgcaata aaaaatggat
    22861 tgcgaaaact gaccagacgt ccattctgtt ggtggtttat acagcgttca gcgaagccgt
    22921 cgttaatggt atctggcata aagttggctg gggatcattg ctgtttatcg tggtggtcag
    22981 ctgcgttctt ctggctatcg tgattgtagt taacgtcttt atggcacgcc gactgggctt
    23041 caataaggca gatgaaatta ctatcgtctt ttgtggttcg aaaaagagtc tggcaaatgg
    23101 catcccgatg gcaaacattc tgttccccac atcggtgatc ggtatgatgg tgctgcccct
    23161 gatgattttc catcagatcc aattgatggt ctgtgcggtg ctggcgcgtc gatacaaacg
    23221 ccagaccgaa cagttacagg cgcagcagga aagcagcgcc gataaagctt aaagcggacg
    23281 cttcaggggc tggaccagtt gcgtcagccc ctcggtttta atcagcagcg tgatttgcat
    23341 tagctcaccg agcttccctg ccgggaactc atctttgcgg gcaaaccaca gcagatactc
    23401 ctccggcagg tcgattaagc gacgcccttt gtatttgcca aacggcatta tcgtattggc
    23461 tatttcaatc agctgctctt tttccatctc agctacccag caaacgcagc atttccgctt
    23521 cgtcgatgac ttcaatgccc agttcctgcg ccttcgccag tttagatcct gcagcttcac
    23581 ccgctatcac cagatcggtt ttcttcgaca cgctgcccgc gactttcgcg cccagttcga
    23641 ccagtcgagc tttagcgtca tcacgcgaca tctggcttaa gctgcccgta agcaccacgg
    23701 ttttaccagc aaacgggctg tcaatctctt ccgcgttgat aacgatcggc gcaggccagt
    23761 gaacaccttc cgccaacagc tcgctgatga cattgcggtt gctttcttcg gcaaagaagt
    23821 tgtgaacgtg ggatgcaacg acaatgccaa catcaggcac cttttgcagc tcttcaatcg
    23881 aagcggcttc cagcacttcc agcgtgccga aatatgccgc cagacctgct gcggtggcct
    23941 cgccgacttc acggatgcca agtgcataga ggaagcgagc aaaggtggtt tctttcgctt
    24001 tttccagcgc gttaaccacg ttttgtgccg attttggccc catacgctcc agtccggtca
    24061 gtttgcctgc ggtgagtttg aacagatctg ccggagtgtg gacatattct ttttcaacca
    24121 gctgatcgat gattttgtcg cccattccgt caacatccat cgcacggcgg gaaacaaagt
    24181 gtttcagcga ctctttacgc tgcgcaccgc aaatcaggcc accggtacag cgggcaaccg
    24241 cttcaccttc cacacgctca acgtcagaac cacataccgg acaatgcgtc gggaatacaa
    24301 cctcacgggt atcttccggg cgttcagaaa gcacgacgtt aaccacctgc gggatcacgt
    24361 cgccagcgcg gcgaatcacc actttatcgc caatgcgtaa accaagacgt tcgatttcat
    24421 ccgcattgtg taaggttgcg ttactcacca gcacgccagc aacatggaca ggttccagac
    24481 gcgcaacagg cgtaatcgcg ccagtacgcc caacctgaaa ctcgacgtca cgcacaaagg
    24541 tcatctgctc ctgcgccggg aatttaaacg ctaccgccca gcgcggggca cgcgcgacaa
    24601 agccaagctg ctcctgctgt tccagtgagt tgaccttaat caccacgccg tcgatatcaa
    24661 agcccagcgt cgggcggtct tcttccactt tgtgatagaa cgccagcact tcttccgccg
    24721 attcacaaag cgttacccga tcgctgaccg gcaaccccca ctttttaaat tgcagtaaac
    24781 ggccaagatg agtatccggc agctcgccac cttccagaac accaacgcca tagcagaaaa
    24841 aagtgagcgg tcgcttcgct gtaatacgcg gatcaagctg acgcagtgaa ccagctgccg
    24901 cattacgtgg gttagcaaaa actttcccgc ccgtgcgtcg cgcatcttcg ttaatctttt
    24961 cgaaccccgc ctgcggcagg aacacttcac cacgcacttc cagacgcgcc gggatattct
    25021 ctccgtgcag cttcagcgga atggcgcgaa tagtacgcac attagacgtg atatcttccc
    25081 cggtggtgcc atcgccacgg gtcgcggcac tgactaaaac gccattttca tacagaatac
    25141 tgacggcaag gccatccagc ttcagctcac agcaccaggt gactttctcg ttgtttttca
    25201 gacggtcctg cacacgtttg ttgaaagcaa gaaagctttc ttcatcaaaa acgttatcca
    25261 gtgacagcat tggtacttca tggcgtatct ggctgaaagc cgccagcggc gcagcaccta
    25321 cacgttgggt aggcgaatca ggcgtaatca gttctggatg tttggtttcc agctcgcgca
    25381 gttcgcgcat cagcctgtcg tattcagcgt cgggaatttc cggcgcatcc atcacatgat
    25441 aaagatattc atgatggcga agcgtcgttc gcagttctgt cagttgttgt tcgattgatt
    25501 ccatatcgca ccatcaatgc taaaaacccc cgacaagcgg gggttcgaag aggagttaat
    25561 ttgccttaag tgtatcaggc gttggcgtct ttgacttcgc ggatgatgtc ctggtactcg
    25621 cgcaatttct gcggagtcat catacggcgc tgatcgtcaa gcacgacacc gcccacttca
    25681 tcggcaatat gctgcgcaga ttgcagcatc agcttgaagt tctgcagctc gtcaccgtaa
    25741 gacggtacct gcataaagat agtgacaccc ggagtagtga aatccttcat ttcaggatca
    25801 aaggttcccg gtttcaccat attcgccagg ctgaataacg ccgggccgct gccatccggg
    25861 ctaagatgac gatggtaaat attcatatcg ccaaaaatga agcccgcttg ttgaatgctg
    25921 ttaagaagca gttcaccgtt tagctcgcta ccgtgatgcg ccgcgacgtt catgataatc
    25981 accgcttctt tgcgcttcgg tttatccata actggagccg gttccgctac aggctcaggc
    26041 tgtggtgccg ctacgggttc tgcaggctgg aaagcctgtt gtgccggttg cggtgctgaa
    26101 tgcacaggct gcggcgctgg cgcgacctgt ggcgaaactg gctgctgcaa cggctgttca
    26161 ggctgcggct gataggcagg ctgctgcatc ggctgcgctg gacgcggagc atgttgcggc
    26221 ggtacctgcg cttcaggcgg ctgctggatc ggttgacgcg gctgcgcaga cgcataaggc
    26281 ggttggtact ggtgttgcgg cgacggacga gcagcctcat gctcctgagc gttagccggg
    26341 gcatgattca cgcggtgaac acgaacctca ccaacgccct catcatcttc gacatcctcg
    26401 tcataagaat cgtcgtcacg ttttgacttc attcgtttta atggccgatc gcggaacata
    26461 gaagatcgtt ctttacggct ggtccagaaa ccatgtacca gtaaagcgat tatggcgatc
    26521 gcgccaacaa tgattaatat cagacgcaaa tcctgcatca ttatattctc tgttgttcta
    26581 acaccttgcc accacggcaa acatttactc actaagagta tttgccgatt acctcaagtg
    26641 caagtgcact attaactttc acagcacaaa gatagatgaa atcgtgcttt ttgctgtttt
    26701 ttcgaacata tcctaactgt ccattgcgca attacccggt aaaatacgca gaattttcct
    26761 gggattggtc aaaaggagct catcctgatt atggtttcat cattcacatc tgccccacgc
    26821 agcggttttt actattttgc gcaaggctgg aagctcgtct cgcaacctgg gattcggcgt
    26881 ttcgttattt taccgctgct ggtcaatatt ttgttgatgg ggggcgcatt ctggtggctc
    26941 tttacacagc tcgatgtctg gatcccgact ctcatgagtt acgttccgga ctggctacaa
    27001 tggctgagtt atctgttgtg gcctctggct gtcatctctg tgctgttagt gtttggctat
    27061 ttcttctcca cgattgctaa ctggattgcc gctccgttta acggtttatt ggctgaacaa
    27121 ctggaagcac gattgactgg cgctacaccg ccagataccg ggattttcgg tatcatgaaa
    27181 gatgtgccgc gaatcatgaa acgcgaatgg caaaaatttg cctggtatct gccgcgcgca
    27241 attgtattac taattcttta cttcatccct ggtattgggc aaaccgtcgc gccggtactg
    27301 tggttcctgt ttagcgcctg gatgttagcc atccagtatt gcgattaccc cttcgataac
    27361 cacaaagtgc cgtttaaaga gatgcgcacc gccctgcgca cacgcaaaat caccaatatg
    27421 cagtttggtg ctttaaccag cctgtttacg atgatcccgc tgcttaatct gttcatcatg
    27481 cccgttgccg tttgtggcgc gacggcgatg tgggtcgatt gctatcgcga taaacacgcg
    27541 atgtggcggt aacaatctac cggttatttt gtaaaccgtt tgtgtgaaac aggggtggct
    27601 tatgccgccc cttattccat cttgcatgtc attatttccc ttctgtatat agatatgcta
    27661 aatccttact tccgcatatt ctctgagcgg gtatgctacc tgttgtatcc caatttcata
    27721 cagttaagga caggccatga gtaagatttt tgaagataac tcgctgacta tcggtcacac
    27781 gccgctggtt cgcctgaatc gcatcggtaa cggacgcatt ctggcgaagg tggaatctcg
    27841 taaccccagc ttcagcgtta agtgccgtat cggtgccaac atgatttggg atgccgaaaa
    27901 gcgcggcgtg ctgaaaccag gcgttgaact ggttgaaccg accagcggta ataccgggat
    27961 tgcactggcc tatgtagctg ccgctcgcgg ttacaaactc accctgacca tgccagaaac
    28021 catgagtatt gaacgccgca agctgctgaa agcgttaggt gcaaacctgg tgctgacgga
    28081 aggtgctaaa ggcatgaaag gcgcaatcca aaaagcagaa gaaattgtcg ccagcaatcc
    28141 agagaaatac ctgctgctgc aacaattcag caatccggca aaccctgaaa ttcacgaaaa
    28201 aaccaccggt ccggagatct gggaagatac cgacggtcag gttgatgtat ttattgctgg
    28261 cgttgggact ggcggtacgc tgactggcgt cagccgctac attaaaggca ccaaaggcaa
    28321 gaccgatctt atctctgtcg ccgttgagcc aaccgattct ccagttatcg cccaggcgct
    28381 ggcaggtgaa gagattaaac ctggcccgca taaaattcag ggtattggcg ctggttttat
    28441 cccggctaac ctcgatctca agctggtcga taaagtcatt ggcatcacca atgaagaagc
    28501 catttctacc gcccgtcgtc taatggaaga agaaggtatt cttgcaggta tctcttctgg
    28561 agcagctgtt gccgcggcgt tgaaactaca agaagatgaa agctttacca ataagaatat
    28621 tgtggttatt ctaccatcat cgggtgagcg ttatttaagc accgcattgt ttgccgatct
    28681 cttcactgag aaagaattgc aacagtaatg ccagcttgtt aaaaatgcgt aaaaaagcac
    28741 ctttttaggt gcttttttgt ggcctgcttc aaactttcgc ccctcctggc attgattcag
    28801 cctgtcggaa ctggtattta accagactaa ttattttgat gcgcgaaatt aatcgttaca
    28861 ggaaaagcca aagctgaatc gattttatga tttggttcaa ttcttccttt aacggcataa
    28921 tgtttaatga cgtacgaaac gtcagcggtc aacacccgcc agcaatggac tgtattgcgc
    28981 tcttcgtgcg tcgcgtctgt taaaaactgg cgctaacaat acaggctaaa gtcgaaccgc
    29041 caggctagac tttagttcca caacactaaa cctataagtt ggggaaatac aatgttccag
    29101 caagaagtta ccattaccgc tccgaacggt ctgcacaccc gccctgctgc ccagtttgta
    29161 aaagaagcta agggcttcac ttctgaaatt actgtgactt ccaacggcaa aagcgccagc
    29221 gcgaaaagcc tgtttaaact gcagactctg ggcctgactc aaggtaccgt tgtgactatc
    29281 tccgcagaag gcgaagacga gcagaaagcg gttgaacatc tggttaaact gatggcggaa
    29341 ctcgagtaat ttcccgggtt cttttaaaaa tcagtcacaa gtaaggtagg gttatgattt
    29401 caggcatttt agcatccccg ggtatcgctt tcggtaaagc tctgcttctg aaagaagacg
    29461 aaattgtcat tgaccggaaa aaaatttctg ccgaccaggt tgatcaggaa gttgaacgtt
    29521 ttctgagcgg tcgtgccaag gcatcagccc agctggaaac gatcaaaacg aaagctggtg
    29581 aaacgttcgg tgaagaaaaa gaagccatct ttgaagggca tattatgctg ctcgaagatg
    29641 aggagctgga gcaggaaatc atagccctga ttaaagataa acacatgaca gctgacgcag
    29701 ctgctcatga agttatcgaa ggtcaggctt ctgccctgga agagctggat gatgaatacc
    29761 tgaaagaacg tgcggctgac gtacgtgata tcggtaagcg cctgctgcgc aacatcctgg
    29821 gcctgaagat tatcgacctg agcgccattc aggatgaagt cattctggtt gccgctgacc
    29881 tgacgccgtc cgaaaccgca cagctgaacc tgaagaaggt gctgggtttc atcaccgacg
    29941 cgggtggccg tacttcccac acctctatca tggcgcgttc tctggaactg cctgctatcg
    30001 tgggtaccgg tagcgtcacc tctcaggtga aaaatgacga ctatctgatt ctggatgccg
    30061 taaataatca ggtttacgtc aatccaacca acgaagttat tgataaaatg cgcgctgttc
    30121 aggagcaagt ggcttctgaa aaagcagagc ttgctaaact gaaagatctg ccagctatta
    30181 cgctggacgg tcaccaggta gaagtatgcg ctaacattgg tacggttcgt gacgttgaag
    30241 gtgcagagcg taacggcgct gaaggcgttg gtctgtatcg tactgagttc ctgttcatgg
    30301 accgcgacgc gctgcccact gaagaagaac agtttgctgc ttacaaagca gtggctgaag
    30361 cgtgtggctc gcaggcggtt atcgttcgta ccatggacat cggcggcgac aaagagctgc
    30421 catacatgaa cttcccgaaa gaagagaacc cgttcctcgg ctggcgcgct atccgtatcg
    30481 cgatggatcg taaagagatc ctgcgcgatc agctccgcgc tatcctgcgt gcctcggctt
    30541 tcggtaaatt gcgcattatg ttcccgatga tcatctctgt tgaagaagtg cgtgcactgc
    30601 gcaaagagat cgaaatctac aaacaggaac tgcgcgacga aggtaaagcg tttgacgagt
    30661 caattgaaat cggcgtaatg gtggaaacac cggctgccgc aacaattgca cgtcatttag
    30721 ccaaagaagt tgatttcttt agtatcggca ccaatgattt aacgcagtac actctggcag
    30781 ttgaccgtgg taatgatatg atttcacacc tttaccagcc aatgtcaccg tccgtgctga
    30841 acttgatcaa gcaagttatt gatgcttctc atgctgaagg caaatggact ggcatgtgtg
    30901 gtgagcttgc tggcgatgaa cgtgctacac ttctgttgct ggggatgggt ctggacgaat
    30961 tctctatgag cgccatttct atcccgcgca ttaagaagat tatccgtaac acgaacttcg
    31021 aagatgcgaa ggtgttagca gagcaggctc ttgctcaacc gacaacggac gagttaatga
    31081 cgctggttaa caagttcatt gaagaaaaaa caatctgcta atccacgaga tgcggcccaa
    31141 tttactgctt aggagaagat catgggtttg ttcgataaac tgaaatctct ggtttccgac
    31201 gacaagaagg ataccggaac tattgagatc attgctccgc tctctggcga gatcgtcaat
    31261 atcgaagacg tgccggatgt cgtttttgcg gaaaaaatcg ttggtgatgg tattgctatc
    31321 aaaccaacgg gtaacaaaat ggtcgcgcct gtagacggta ccattggtaa aatctttgaa
    31381 accaatcacg cgttctctat cgaatctgat agcggcgttg aactgttcgt ccacttcggt
    31441 atcgacaccg ttgaactgaa aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc
    31501 gtgaaagttg gcgatactgt cattgaattt gatctgccgc tgctggaaga gaaagccaag
    31561 tctaccctga ctccggttgt tatctccaac atggacgaaa tcaaagaact gatcaaactg
    31621 tccggtagcg taaccgtggg tgaaaccccg gttatccgca tcaagaagta attcttgccg
    31681 cagtgaaaaa tggcgccca
//
LOCUS       DAZOXR010000030        31633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZOXR010000030 DAZOXR010000000
VERSION     DAZOXR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.030"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            162..497
                     /gene="eutS"
                     /locus_tag="ACHUNN_001178"
     CDS             162..497
                     /gene="eutS"
                     /locus_tag="ACHUNN_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0575607.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            510..989
                     /gene="eutP"
                     /locus_tag="ACHUNN_001179"
     CDS             510..989
                     /gene="eutP"
                     /locus_tag="ACHUNN_001179"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0575608.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            964..1665
                     /gene="eutQ"
                     /locus_tag="ACHUNN_001180"
     CDS             964..1665
                     /gene="eutQ"
                     /locus_tag="ACHUNN_001180"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR0575609.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1662..2465
                     /gene="eutT"
                     /locus_tag="ACHUNN_001181"
     CDS             1662..2465
                     /gene="eutT"
                     /locus_tag="ACHUNN_001181"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR0575610.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            2462..3478
                     /gene="pta"
                     /locus_tag="ACHUNN_001182"
     CDS             2462..3478
                     /gene="pta"
                     /locus_tag="ACHUNN_001182"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0575611.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3517..3810
                     /gene="eutM"
                     /locus_tag="ACHUNN_001183"
     CDS             3517..3810
                     /gene="eutM"
                     /locus_tag="ACHUNN_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR0575612.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3917..4204
                     /gene="eutN"
                     /locus_tag="ACHUNN_001184"
     CDS             3917..4204
                     /gene="eutN"
                     /locus_tag="ACHUNN_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR0575613.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4216..5619
                     /locus_tag="ACHUNN_001185"
     CDS             4216..5619
                     /locus_tag="ACHUNN_001185"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR0575614.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            5630..6466
                     /gene="eutJ"
                     /locus_tag="ACHUNN_001186"
     CDS             5630..6466
                     /gene="eutJ"
                     /locus_tag="ACHUNN_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR0575615.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6456..7643
                     /gene="eutG"
                     /locus_tag="ACHUNN_001187"
     CDS             6456..7643
                     /gene="eutG"
                     /locus_tag="ACHUNN_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR0575616.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7960..9186
                     /gene="eutH"
                     /locus_tag="ACHUNN_001188"
     CDS             7960..9186
                     /gene="eutH"
                     /locus_tag="ACHUNN_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR0575617.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            9183..10586
                     /gene="eutA"
                     /locus_tag="ACHUNN_001189"
     CDS             9183..10586
                     /gene="eutA"
                     /locus_tag="ACHUNN_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0575618.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10598..11959
                     /gene="eutB"
                     /locus_tag="ACHUNN_001190"
                     /pseudo
     CDS             10598..11959
                     /gene="eutB"
                     /locus_tag="ACHUNN_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            11980..12867
                     /gene="eutC"
                     /locus_tag="ACHUNN_001191"
     CDS             11980..12867
                     /gene="eutC"
                     /locus_tag="ACHUNN_001191"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR0575619.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12877..13536
                     /gene="eutL"
                     /locus_tag="ACHUNN_001192"
     CDS             12877..13536
                     /gene="eutL"
                     /locus_tag="ACHUNN_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0575620.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13549..14049
                     /gene="eutK"
                     /locus_tag="ACHUNN_001193"
     CDS             13549..14049
                     /gene="eutK"
                     /locus_tag="ACHUNN_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0575621.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            14095..15147
                     /gene="eutR"
                     /locus_tag="ACHUNN_001194"
     CDS             14095..15147
                     /gene="eutR"
                     /locus_tag="ACHUNN_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR0575622.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15153..16052)
                     /gene="hemF"
                     /locus_tag="ACHUNN_001195"
     CDS             complement(15153..16052)
                     /gene="hemF"
                     /locus_tag="ACHUNN_001195"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0575623.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16056..16925)
                     /gene="amiA"
                     /locus_tag="ACHUNN_001196"
     CDS             complement(16056..16925)
                     /gene="amiA"
                     /locus_tag="ACHUNN_001196"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0575624.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            17139..17564
                     /locus_tag="ACHUNN_001197"
     CDS             17139..17564
                     /locus_tag="ACHUNN_001197"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0575625.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17551..18000
                     /locus_tag="ACHUNN_001198"
     CDS             17551..18000
                     /locus_tag="ACHUNN_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0575626.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            18061..18636
                     /locus_tag="ACHUNN_001199"
     CDS             18061..18636
                     /locus_tag="ACHUNN_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0575627.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18732..19631
                     /gene="yfeX"
                     /locus_tag="ACHUNN_001200"
     CDS             18732..19631
                     /gene="yfeX"
                     /locus_tag="ACHUNN_001200"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0575628.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19689..20993)
                     /gene="pbp4b"
                     /locus_tag="ACHUNN_001201"
     CDS             complement(19689..20993)
                     /gene="pbp4b"
                     /locus_tag="ACHUNN_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR0575629.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20998..22422)
                     /gene="murP"
                     /locus_tag="ACHUNN_001202"
                     /pseudo
     CDS             complement(20998..22422)
                     /gene="murP"
                     /locus_tag="ACHUNN_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(22426..23322)
                     /gene="murQ"
                     /locus_tag="ACHUNN_001203"
     CDS             complement(22426..23322)
                     /gene="murQ"
                     /locus_tag="ACHUNN_001203"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0575630.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23486..24343
                     /gene="murR"
                     /locus_tag="ACHUNN_001204"
     CDS             23486..24343
                     /gene="murR"
                     /locus_tag="ACHUNN_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR0575631.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24472..25263
                     /gene="ucpA"
                     /locus_tag="ACHUNN_001205"
     CDS             24472..25263
                     /gene="ucpA"
                     /locus_tag="ACHUNN_001205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0575632.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25434..26450
                     /gene="cysP"
                     /locus_tag="ACHUNN_001206"
     CDS             25434..26450
                     /gene="cysP"
                     /locus_tag="ACHUNN_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0575633.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26450..27283
                     /gene="cysT"
                     /locus_tag="ACHUNN_001207"
     CDS             26450..27283
                     /gene="cysT"
                     /locus_tag="ACHUNN_001207"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0575634.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27283..28158
                     /gene="cysW"
                     /locus_tag="ACHUNN_001208"
     CDS             27283..28158
                     /gene="cysW"
                     /locus_tag="ACHUNN_001208"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0575635.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            28148..29245
                     /gene="cysA"
                     /locus_tag="ACHUNN_001209"
     CDS             28148..29245
                     /gene="cysA"
                     /locus_tag="ACHUNN_001209"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0575636.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29379..30290
                     /gene="cysM"
                     /locus_tag="ACHUNN_001210"
     CDS             29379..30290
                     /gene="cysM"
                     /locus_tag="ACHUNN_001210"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0575637.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30293..30661)
                     /locus_tag="ACHUNN_001211"
     CDS             complement(30293..30661)
                     /locus_tag="ACHUNN_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0575638.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30766..31617
                     /gene="pdxK"
                     /locus_tag="ACHUNN_001212"
                     /gene_synonym="thiJ"
     CDS             30766..31617
                     /gene="pdxK"
                     /locus_tag="ACHUNN_001212"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0575639.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         7163 a         8172 c         9148 g         7150 t
ORIGIN      
        1 atttatattc atgcgttgca tatgaaagtt tatgcaccac agcgaatatc tctccattct
       61 tagtgatcta cctcaccttt taaacgcgct tgccgaattt tgttatttac tctgacgaaa
      121 aattgtcacg atacacgaaa gtttttcaca ggcggcgact catggataaa gaacgcatca
      181 ttcaggaatt tgtgcccggc aaacaggtca cactggcgca tctcatcgcg catcccggtg
      241 aggaactggc gaagaaaatc ggcgttccgg atgcgggcgt aattggcatt atgacgctaa
      301 ctcccggcga aacggcgatg atcgtcggtg atttagcgct gaaagccgct gatgtgcata
      361 tcggttttct cgaccgcttc agcggtgcat tggtgattta tggctcggtg ggcgcagtag
      421 aagaagcgtt atcacaaacc gtcagcggtt taggtcgctt attaaattat acgctttgtg
      481 aaatgaccaa aagttaatac gaggcattca tgaaacgaat tgcgtttgtg ggtagcgtcg
      541 gggcaggaaa aacaacgtta tttaatgcgt tgcaggggaa ttataccctc gccagaaaaa
      601 cacaggccgt ggaatttaat gataagggcg atattgatac gccgggtgaa tattttagcc
      661 atccccgctg gtatcacgcc ttaattacca cgctacagga tgtagacatg ctgatctatg
      721 tccatggcgc aaatgacccg gaaagtcggt tgcctgccgg gttgctggat attggcgtca
      781 gcaaacggca aatcgccgtc atcagtaaaa cggacatgcc agatgctgac gtcgccgcaa
      841 cgcgaaagtt attgcttgag acgggtttcg aagagccgat tttcgagcta aacagccacg
      901 acccgcaaag cgtgcagcaa ctggtggatt atctggcatc tctcaccaaa caggaggaag
      961 caggtgaaaa aactcatcac agcgaatgat attcgtgaag cacacgcgcg cggcgaacag
     1021 gcaatgtccg tggttctgcg cgccagcatt attaccccgg aagcccgtaa agtggcggat
     1081 ctactgggtt ttaccatcac cgaatgtgat gaatcgatcc cggtaacggc gtccgtgccc
     1141 gccagcgtgc cagccgacaa aaccgaaagc cagcgcatcc gcgaaaccat cattgcccag
     1201 ctgccggaag gccagtttac cgaaagcctg gtcgcacagc tgatggaaaa agtgatgaag
     1261 gaaaaacagt cgctggagca gggcgcaatg cagccgagct tcaaatcggt gaccggcaaa
     1321 ggcggcatca aagtgattga tggcagcagc gtcaagtttg gtcgttttga tggcgcacaa
     1381 ccgcattgcg tgggcttgac tgatcttgtg accggagacg acggcagcag catggccgcc
     1441 ggttttatgc agtgggagaa cgcattcttc ccgtggaccc tgaactacga cgaaatcgac
     1501 atggtgctgg aaggggagct ccatgttcgc cacgaaggcg aaaccatgat cgccaaagca
     1561 ggcgacgtga tgtttatccc gaaaggctcc agcatcgaat ttggtacgac atccagcgtg
     1621 aaattcctgt atgtcgcctg gccggctaac tggcaatccc tatgaaagat ttcatcaccg
     1681 aagcatggct aagagcgaac catacgctca gcgaaggcgc agagatccat ctccctgcgg
     1741 acagtcgcct gacgccctct gcccgggagt tgctggaaag ccgccatctg cgcatcaagt
     1801 ttattgacga gcagggccgc ctgtttgttg acgatgaaca gcagcagccg cagcccgttc
     1861 atgggctgac cagtagcgat gaacatccgc aggcgtgctg cgaactgtgc cgccagccgg
     1921 tggcaaaaaa gccggatacg ctgactcacc tgtcggcgga aaaaatggtc gccaaaagcg
     1981 atccgcgtct gggttttcgc gcggttctcg acagcaccat tgcgctggca gtgtggctgc
     2041 aaattgaact ggcggaaccg tggcagccgt ggctggcgga tatccgttcg cgtcttggca
     2101 acattatgcg cgccgatgcg ctgggggaac cgctgggctg ccaggcgatt gtcgggcttt
     2161 ctgacgaaga tctgcatcga ctttctcacc agccgctgcg ctatctcgac cacgatcatc
     2221 tggtgccgga agccagccac gggcgcgatg cggcgttgct caatctgcta cgtaccaaag
     2281 tgcgggaaac cgaaaccgta gcggcgcagg tgtttatcac ccgcagcttt gaagtattac
     2341 gcccggacat cttgcaggcg ctgaaccgcc tttcgagcac ggtctacgta atgatgatcc
     2401 tgagcgtaac gaagccgccg ctaacggtga agcagatcca acaacgactg ggggaaacgc
     2461 aatgattatt gaacgttgtc gtgaactggc gttgcgagcg cccgccagag tggtttttcc
     2521 ggatgcgtta gatcaacgtg tgctgaaagc tgcgcaatat ttacatcaac aaggtctggc
     2581 aacgcccatt ctggtcgcta atccgtttga acttcgtcag tttgcgctca gtcacggcgt
     2641 ggcgatggac gggctacagg tgattgatcc gcatggcaac ctcgcaatgc gggaagaatt
     2701 tgctcatcgc tggctggccc gcgcgggcga aaaaacgccg ccggatgcgc tggaaaaact
     2761 taccgatccg ctgatgttcg ccgcagcaat ggtcagcgcc ggtaaagcgg atgtctgtat
     2821 cgcgggcaac ctctcttcca cggcgaatgt gctgcgtgcc ggattacgta ttatcggctt
     2881 gcagccaggc tgtaaaacgc tctcatccat tttcctgatg ctgccacagt acagcggtcc
     2941 ggcgttgggc tttgccgatt gcagcgtggt gccacagccg acggcggcgc aactggcgga
     3001 tatcgcgctt gccagtgccg aaacctggcg cgccctcacc ggagaagagc cgcgcgtggc
     3061 gatgctgtcg ttttccagta acggtagcgc ccgtcacccc tgcgttgcca acgtccagca
     3121 ggcgacagaa atcgtccgtg agcgcgcacc aaagctggtg gttgatggcg agttgcagtt
     3181 tgacgccgcc ttcgtgccgg aagtggcggc gcaaaaagcg cctgccagcc cgctacaggg
     3241 caaagccaat gtgatggttt ttccgtcgct ggaagccgga aatattggct acaaaatcgc
     3301 acaacgactc ggcggatatc gtgccgtcgg gccattgata caaggacttg ccgcgccgat
     3361 gcacgatctc tctcgtggtt gtagcgtgca ggaaattatc gagctggcgc tggtggcagc
     3421 tgtgccgcgt cagacagaag tgaaccgcga aagcagttta caaacactgg ttgaatgaac
     3481 ggtcccgttc tggaccctga taaagaggaa agcacaatgg aagcattagg aatgatcgaa
     3541 acccggggcc tggttgcact gattgaggct tctgacgcga tggttaaagc tgcgcgcgtc
     3601 aagctggttg gtgttaagca gattggcggc ggcctgtgta ctgccatggt tcgtggtgat
     3661 gttgcagcct gtaaagccgc gaccgatgca ggtgccgcag cggcacagcg tatcggcgag
     3721 ctggtatctg tacacgttat cccgcgtccg cacggcgacc tggaagaagt cttcccgatc
     3781 ggcctgaaag gcgatagcag caacctgtaa tcaccctgtg tgacgtcgat tcgggcgtca
     3841 cgctctctcc tttttcattt taccttctgc ggagggtagg gggagctttt acctgaaata
     3901 cgccacggag gcgggtatga aactggcagt cgtcactgga caaattgttt gtaccgtacg
     3961 ccatcacgga ctggcgcatg acaaattgct gatggtggaa atgattgatc cacaaggtaa
     4021 tcctgacggg caatgcgccg tcgccatcga caatattggc gcgggaaccg gggagtgggt
     4081 gttactggtg agtggcagtt ccgcccgcca ggcgcataaa agcgaaacgt caccggtcga
     4141 tctgtgcgtg attggcattg tcgatgaggt ggtgtctggc ggtcaggtaa ttttccacaa
     4201 ataaggcaga acatcatgaa tcaacaggat attgaacagg tggtgaaagc ggtactgctg
     4261 aaaatgcaaa gcagtgacac gccgcccgcc gccgttcatg agatgggcgt tttcgcgtcc
     4321 ctggatgacg ccgttgcggc agccaaagtc gcccagcaag ggttaaaaag cgtggcaatg
     4381 cgccagttag ccattgctgc cattcgtgag gcaggcgaaa aacacgccag agatttagcg
     4441 gaacttgccg tcagtgaaac cggcatgggc cgcgttgaag ataaatttgc caaaaacgtc
     4501 gctcaggcgc gcggcacgcc aggcgttgag tgtctctctc cgcaggtgct gaccggcgac
     4561 aacggcctga ctttaattga aaacgcgccc tggggcgtgg tggcttcggt aactccgtcc
     4621 actaacccgg cggcaaccgt aattaataac gccatcagcc tgattgccgc gggcaacagc
     4681 gtcatttttg ccccgcatcc ggcggcgaaa aaagtctccc agcgggcgat cacgctgctc
     4741 aaccaggcga ttgttgccgc aggcgggccg gaaaacttac tggttactgt ggcaaatccg
     4801 gatatcgaaa ccgcgcagcg tttgttcaag tttccgggca tcggccttct ggtggtgacc
     4861 ggcggcgaag cggtagtaga agcggcgcgt aaacacacca ataaacgtct gattgccgca
     4921 ggcgctggca acccgccggt agtggtggat gaaaccgccg acctcgcccg tgccgctcag
     4981 tccatcgtca aaggcgcttc tttcgataac aacatcattt gtgccgacga aaaggtactg
     5041 attgttgttg atagcgtagc cgatgaactg atgcgtctga tggaaggcca gcacgcggtg
     5101 aaactgaccg cagaacaggc gcagcagctg caaccggtgt tgctgaaaaa tatcgacgag
     5161 cgcggaaaag gcaccgtcag ccgtgactgg gttggtcgcg atgcggcgaa aatcgcggcg
     5221 gcaatcggcc tgaacgtgcc gcaagaaacg cgtttgctgt ttgtggaaac cactgcagaa
     5281 catccgtttg ccgtgaccga actgatgatg ccggtgctgc ccgtcgtgcg cgtcgccaac
     5341 gtggcggatg ccattgcgct ggcggtgaaa ctggaaggcg gttgccacca cacggcggca
     5401 atgcactcgc gcaacatcga aaacatgaac cagatggcga atgctattga taccagcatt
     5461 ttcgttaaga acggaccgcg cattgccggg ctggggctgg gcggggaagg ctggaccacc
     5521 atgaccatca ccacgccaac cggtgaaggg gtaactagcg tgcgtacgtt tgtccgtctc
     5581 cgtcgctgtg tattagtcga tgcgtttcgc attgtttaag gagtaacaga tggcgcacga
     5641 cgaacaatgg ctcaccccac gtctgcaaac ggcggcaacg ctgtgtaacc agacgcccgc
     5701 cgcgacggaa tctccgctgt ggctgggggt ggatctgggc acctgcgatg tggtgtcgat
     5761 ggttgtcgac cgcgacggtc agtcggtggc ggtatgcctc gactgggccg atgtggtccg
     5821 cgacggcatc gtctgggatt tcttcggcgc tgtcaccatt gttcgtcgcc atctcgacac
     5881 gctcgagcag caattcggtc gccgttttag ccatgcggcg acttcatttc ctccaggtac
     5941 ggacccgcgt atttccatca acgtgctgga gtctgctgga ctggaagtga gccatgtgct
     6001 ggatgagcca accgcagttg cggatctgct gcaactggac aacgccggtg tagtggatat
     6061 cggcggcggc actaccggca ttgccatcgt gaaaaagggc aaggtgacgt actcggcgga
     6121 tgaagcaacc ggcggacatc acatctctct gactctcgcc ggaaatcgcc gtatttcgct
     6181 ggaagaggcg gagcagtaca agcgcggtca cggtgaagag atttggccta cggtgaaacc
     6241 ggtgtacgaa aaaatggcgg acattgtcgc ccggcatatt gaagggcagg gcattactga
     6301 tttatggctg gcgggcggct cttgtatgca accgggcgtg gcggaactgt ttcgcaaaca
     6361 attcccggcg ttacaggtgc atttaccgca gcacagtttg tttatgaccc cgctggcgat
     6421 cgccagtagt gggcgagaga aagcggaggg gctatatgca aaatgaattg cagaccgcgc
     6481 tctttcaggc gttcgatacc ctgaatctgc aacgggtaaa aacatttagc gttccaccgg
     6541 tgacgctttg cggtccgggc tcggtgagca gttgcggaca gcaagcgcaa acgcgtgggc
     6601 tgaaacatct gttcgtgatg gcagacagct ttttgcatca ggcagggatg accgccgggc
     6661 tgacgcgcag cctggctgtt aaaggcatcg ccatgacgct ctggccatgt ccggtgggcg
     6721 aaccgtgcat caccgacgtg tgtgcagccg tggcgcagtt gcgtgagtca ggctgtgatg
     6781 gggtgatcgc atttggcggc ggctcggtgc tggatgcggc gaaagccgtg gcgttgctgg
     6841 tgacgaaccc ggatagcacg ctggcagaga tgtcagaaac cagcgttctg caaccgcgct
     6901 tgccgctgat tgccattcca acgaccgccg gaaccggctc tgaaaccacc aatgtaacgg
     6961 tgattatcga cgcggtgagc gggcgcaagc aggtgttagc ccatgcctcg ctgatgccgg
     7021 atgtggcgat tctcgacgcc gcgttgaccg aaggtgtgcc gtcgcatgtc acggcgatga
     7081 ccggcattga tgcgttaacc catgccattg aagcatacag cgccctgaac gctacaccgt
     7141 ttaccgacag tctggcgatt ggtgccattg cgatgattgg caaatcgctg ccgaaagcgg
     7201 tgggctacgg tcacgacctt gccgcgcgcg agagcatgtt gctggcttca tgtatggcgg
     7261 gaatggcgtt ttccagtgcg ggtcttgggt tgtgccacgc gatggcgcat cagccgggcg
     7321 cggcgctgca tattccacac ggtctggcga acgccatgtt gctgccaacg gtgatggaat
     7381 ttaaccggat ggtttgtcgt gaacgcttta gtcagattgg tcgggcactg cgaactaaaa
     7441 aatccgacga tcgtgacgct attaacgcgg taagtgagct gattgcggaa gttgggattg
     7501 gtaaacgact gggcgatgtt ggtgcgacat ctgcgcatta cggcgcatgg gcgcaggccg
     7561 cgctggaaga tatttgtctg cgcagtaacc cgcgtaccgc cagcctggaa cagattgtcg
     7621 gactgtacgc ggcggcgcaa taaatgccgg atgcgacgct tgccgcgtct tatccagcct
     7681 acgggattgc acatgtaggg cggataaggc gttcacgccg catccgccag tggcgcggtg
     7741 caattgccgg atgcgatgct tgccgcgtct tatccggcct acgggattgc acatgtaggg
     7801 cggataaggc gttcacgccg catccgccag tggcgcggtg caattgccgg atgcgacgct
     7861 tgccgcgtct gatccggcct tcgggattgc acatgtaggg cggataaggc gtttacgccg
     7921 catccgccaa taaataacaa ctaacaggga gtaaaggcga tgggaattaa cgaaatcatc
     7981 atgtacatca tgatgttctt tatgctgata gctgccgtag acaggatcct gtcgcagttc
     8041 ggcggttctg ctcgtttcct cggtaagttc ggtaaaagta tcgaaggatc aggcggtcag
     8101 ttcgaagaag gctttatggc aatgggcgct ctgggtctgg cgatggtcgg tatgaccgcg
     8161 ctggcaccgg tactggctca cgtactcggg ccggtaatta tcccggttta cgaaatgctc
     8221 ggcgcaaacc cgtcaatgtt cgccggaacg ctgctggcgt gcgatatggg cggcttcttc
     8281 ctcgccaaag agctggcggg cggcgacgta gcagcgtggc tatactctgg gttaattctt
     8341 gggtcgatga tggggccaac gattgtgttt tccattccgg tggcgctcgg cattatcgaa
     8401 ccttctgacc gtcgttatct ggcgctcggc gtgctggcgg gcattgtgac cattccgatt
     8461 ggctgtattg ctggtggtct ggttgctatg tactccggtg tgcagatcaa cggtcagccg
     8521 gtggaattca ctttcgccct gatcctgatg aacatgatcc cggtgatcat tgttgcgatt
     8581 ctggtggcgc tggggctgaa attcatcccg gaaaaaatga tcaacggctt ccagatcttc
     8641 gccaaattcc tcgttgcatt gatcaccctc ggtcttgctg ccgcggtagt taagtttctg
     8701 cttggctggg aactgatccc cggtctggac cctatcttta tggcccctgg cgataaaccc
     8761 ggtgaagtga tgcgcgccat tgaagttatc ggttctatct cctgcgttct gttaggggcg
     8821 tatccaatgg tgctgctgct gacccgctgg tttgaaaaac cgctgatgag cgtcggtaaa
     8881 gtgctgaata tgaacaacat cgcggcagcc ggcatggtgg caacgcttgc caacaacatc
     8941 ccgatgttcg gcatgatgaa gcagatggat acccgcggca aagtcatcaa ctgcgccttc
     9001 gccgtttccg ctgctttcgc cctgggcgac cacttaggct tcgccgctgc caacatgaac
     9061 gccatgatct tcccgatgat tgtcggcaag ttgatcggcg gcgtaacggc gattggcgtg
     9121 gcgatgatgc tggtgccaaa agaagacgcg accacggcta aaaccgaagc ggaggcacaa
     9181 tcgtgaacac tcgccagcta ttgagcgtcg gtatcgatat cggcaccacc accacccagg
     9241 tgattttctc ccacctggag ctggttaacc gtgcggcggt gtcgcaggtg ccgcgctacg
     9301 aattcattaa acgcgaaatt agctggcaaa gcccggtgtt ctttacccct gtcgataaac
     9361 agggtggttt aaaagaagcg gaactgaaaa ccttaatcct cgagcaatat caggctgcgg
     9421 gcattgcgcc ggaaagcgtt gattctggtg ccatcatcat caccggtgaa agcgcgaaaa
     9481 cccgcaatgc tcgtccggcg gtgatggtgc tctcccagtc gctgggcgat tttgtcgttg
     9541 ccagcgccgg gccgcacctc gaatccgtca ttgccggtca cggtgccggg gcacaaaccc
     9601 tttctgaaca acggctgtgt cgggtactga atattgacat cggcggtggc actgcgaact
     9661 acgccctgtt cgatgccgga aaaatcagcg gcactgcctg cctcaacgtc ggtggtcgcc
     9721 tgctggaaac cgacagccag gggcgcgtgg tttacgctca taaaccgggg cagatgattg
     9781 tggatgagtg cttcggcgca ggcaccgacg cccgttcgct gaccggcgcg cagctggtgc
     9841 aggttacccg gcggatggca gaactgattg tcgaagtgat tgacggaacg ctctcgccgc
     9901 tcgcgcaggc attgatgcaa accggtttgc tgcccgcagg tgttacgccc gaaatcatta
     9961 cgctttctgg cggcgtgggc gaatgttatc gccaccagcc cgccgacccg ttctgttttg
    10021 ccgatattgg cccgctgctg gcaacggcgc tgcatgacca tccgcgcctg cgtgagatga
    10081 acgtacagtt tccggcgcaa accgtgcgcg ccacggtgat tggcgcgggc gcgcataccc
    10141 tttcgctctc tggcagcaca atctggcttg agggcgtaca actgccgctg cgcaatttgc
    10201 cggtggcgat cccgattgat gaaacggatc tggtgagtgc ctggcaacag gcgctgcttc
    10261 agctggatct tgatcccaaa actgacgcgt acgtgctggc gcttcccgcc tcgctgcctg
    10321 tgcgttacgc cgcggtactg acggtcatca acgcgctggt cgatttcgtc gcgcgttttc
    10381 caaatccgca tcccctgctg gtggtggccg ggcaggactt tggtaaagct ctgggcatgt
    10441 tgttgcgccc acagctacaa caactcccgt tggcagtcat tgacgaagtg attgtccgcg
    10501 cgggggacta tatcgacatt ggtacgcctc tttttggcgg atcggttgtg ccggtgacgg
    10561 tgaaatcact cgcatttcct tcctgaggga acgacttatg aaactaaaga ccacattgtt
    10621 cggcaatgta tatcagttta aggatgtaaa agaggtgctg gctaaagcca acgaactgcg
    10681 ttcgggggat gtgctggcgg gcgttgcagc ggcaagctca caggagcgcg tggcggcaaa
    10741 gcaggtgttg tcggaaatga ccgtagcgga catccgcaat aatccggtga ttgcctatga
    10801 agatgactgc gtgacgcggc tgattcagga cgatgttaac gaaacggcct acaaccagat
    10861 taaaaacagg agcatcagcg aactgcgtga gtatgtgctg agcgatgaaa ccagcgtgga
    10921 cgacattgcc tttacccgca aagggctgac ctcggaagtg gtcgcggcgg tagcgaagat
    10981 ttgctccaac gcggacctga tctacggcgc gaagaaaatg ccggtaatca aaaaggccaa
    11041 taccaccatc ggtattccgg gcaccttcag cgcccgtttg cagccaaatg acacccgtga
    11101 cgacgtgcaa agtatcgctg cacaaatcta cgaagggctt tccttcgggg tgggcgatgc
    11161 ggtgatcggc gttaacccgg taactgacga cgtggaaaac ttaagccgcg tgttggacac
    11221 catttacggc gtgatcgaca aattcaacat cccaactcag ggctgcgtac tggcgcacgt
    11281 caccacccag atcgaagcga ttcgtcgcgg cgcgcctggc ggactgattt tccagagtat
    11341 ctgtggcagc gaaaaagggc tgaaagagtt tggcgtggaa ctggcgatgc tcgacgaagc
    11401 gcgcgcagtg ggtgctgagt tcaaccgtat cgccggggaa aactgcctct acttcgaaac
    11461 cgggcaaggt tcggcgctgt ctgctggcgc taacttcggc gcagactagg tgacgatgga
    11521 agcacgtaac tatgggctgg cgcgtcatta cgatccgttt atcgtcaaca ccgtggtcgg
    11581 ctttattggg ccggagtatc tctacaacga ccgccagatt atccgtgctg gcttagaaga
    11641 tcactttatg ggcaagctga gcggcatctc tatgggctgt gactgctgtt acaccaacca
    11701 cgctgatgct gaccagaacc tcaacgaaaa cctgatgatc ctgctcgcca ccgcaggctg
    11761 caactacatc atggggatgc cgctgggcga tgacatcatg ctcaactatc agaccaccgc
    11821 attccacgac actgccactg tgcgtcagtt actcaacctg cgcccgtcac cggagtttga
    11881 acgctggctg gaaagcatgg gcattatggc aaacggtcgc ctgaccaaac gggcgggcga
    11941 tccgtcactg ttcttctgat gacgcggaga taacacatca tggatcaaaa acagattgaa
    12001 gaaattgtac gcagcgtgat ggcgtcaatg ggacaagcgg cccccgcgcc gtcagaagca
    12061 aagtgcgcca ccactaactg tgcggcaccg gtgacctcgg aaagctgcgc gctggattta
    12121 ggttctgcgg aagcaaaagc gtggattggt gttgaaaacc cgcatcgcgc agacgtatta
    12181 acagaactgc gccgcagcac cgtggcccgc gtttgtaccg gtcgtaccgg tccgcgtcca
    12241 cgtacgcagg cgctgctgcg tttcctggcc gatcactctc gttcgaaaga taccgtactg
    12301 aaagaagtgc cggaagagtg ggtgaaagcg cagggcttgc tggaagtacg ctccgaaatt
    12361 agcgacaaaa atctctacct gactcgcccg gatatgggcc gccgcctgtg tgcagaagct
    12421 gttgaagcgc tgaaagcaca gtgtgttgct aatccggacg tacaggtcgt tatttctgat
    12481 ggcctgtcaa ctgatgccat caccgtgaac tacgaagaga tcctgccgcc gctgatggcg
    12541 ggcctgaaac aggccgggct gaaagtcggc acgccattct ttgttcgtta tggtcgcgtg
    12601 aagattgaag atcagatcgg tgagatcctc ggcgcgaaag tggtgatcct gctggtgggg
    12661 gaacgtccgg ggttgggtca gtcagaaagc ctctcctgtt acgccgttta ctcgccgcgt
    12721 atggcgacca ccgttgaggc cgatcgcacc tgcatctcta acattcacca gggcggcacg
    12781 ccgccggttg aagccgcagc cgtcattgtg gatttggcga aacgtatgct ggagcagaaa
    12841 gcatccggca tcaacatgac ccgataagga ggcatcatgc cagctttaga tttgattcga
    12901 ccgtcggtaa ccgctatgcg ggtgattgcc tccgttaact ctgaatttgc gcgtgaacta
    12961 aaattgccgc cgcatattcg tagcctcggg ctgatttctg ctgattccga tgacgtcacc
    13021 tacattgcgg ctgacgaagc gaccaagcag gcgatggtcg aagtggtata tggccgctcg
    13081 ctgtatgcgg gagcagcaca cggaccgtca ccgactgccg gtgaagtgct gattatgctt
    13141 ggtgggccaa acccggcgga agtgcgtgct ggtctggatg cgatggttgc gcatattgaa
    13201 aatggcgctg ctttccagtg ggctaatgac gcagaaaaca ccgcattcct ggcacatgta
    13261 gtttcgcgta ccggttctta tctttcatca accgccggga tcacgcttgg cgatccgatg
    13321 gcgtatctgg tggcaccgcc gctggaagcg acctacggca ttgatgcagc gttgaaatct
    13381 gccgacgtac agctggtgac ctatgtcccg ccaccgtctg aaaccaacta ctcggcagca
    13441 tttttaaccg gtagccaggc cgcgtgtaaa gcagcctgta acgcctttac cgatgcagtg
    13501 ctggaaatcg cgcgtaatcc aatccagcgt gcgtaacgga ggttgccgat gatcaatgca
    13561 ctgggattgc tggaagtgga cggcatggtc gccgcgatag atgcagcgga tgccatgctc
    13621 aaagcagcta acgttcgtct gctcagtcac gaagtgcttg accctggtcg cttaacgctg
    13681 gtggtggaag gcgatctggc ggcgtgtcgt gcggcgctgg acgcaggttg tgctgccgcg
    13741 atgcgtaccg ggcgtgtcat cagccgcaag gagatcggtc ggccagacga tgacacccag
    13801 tggctggtta ctggctttaa ccgccagccg aagcaacccg taaaggaacc cgacgcgcca
    13861 gttatcgtcg cggaatctgc tgacgagttg ttggcgctgt taacatcagt acgtcaggga
    13921 atgacggcag gagaagtggc tgcccacttt ggctggccgc tggaaaaagc cagaaatgcg
    13981 ctcgaacagc tcttttctgc cgggacgtta cgtaaacgca gtagtcgtta tcgtctcaag
    14041 ccccattaac ctgtcggagg tgccgggtgt cctacaacac ccggcattaa catcatgaaa
    14101 aagacccgta cagccaattt gcaccatctt tatcatgaac ccttacccga aaacctgaag
    14161 ctcacgccga aggtcgaagt ggataatgtt catcaacgac agacaacgga tgtctatgaa
    14221 catgctttga cgattaccgc ctggcagcag atttacgatc agctgcatcc gggcaagttt
    14281 catggtgaat ttacggaaat tctgctcgat gatattcagg tttttcgtga atacaccggt
    14341 ctggcgctgc gtcagtcgtg cctggtctgg ccgaactcgt tctggtttgg cattccggcg
    14401 acgcgcggtg agcagggatt tatcggttcg caatgtctgg gaagcgcaga aatagcgacg
    14461 cgccccggtg gaaccgagtt tgaattaagt acgccggacg attatacgat cctgggcgta
    14521 gtgctgtctg aagatgtcat cacccgtcag gctaactttt tgcacaatcc ggatcgggta
    14581 ttacatatgc tgcgtagcca gtcggcgctg gaagtgaaag agcagcataa agccgcgctg
    14641 tggggctttg tccaacaggc gctggcgacg ttttgcgaga acccggaaaa tctccatcag
    14701 cctgcagtgc gaaaagtgct gggggataat ttgctaatgg cgatgggggc gatgctggaa
    14761 gacgcgcaac caatggtgac ggcggaaagc atcagtcatc agagttaccg tagattactt
    14821 tcccgcgccc gtgaatatgt gctggaaaat atgtctgagc tggtgacggt gctggacttg
    14881 tgtaatcaac tgcatgtcag tcgccgcacg ctacaaaacg cgtttcacgc tattttaggc
    14941 attggcccaa acgcgtggct gaaacgcatt cgcctgaacg ccgtacgccg cgaactgata
    15001 agcccgtggt cgcaaagcac aacggtaaaa gacgccgcca tgcagtgggg attctggcat
    15061 ctggggcaat ttgccacgga ttaccagcag ctgtttgccg agaagccgtc gttgacgttg
    15121 catcagcgga tgcgggaatg ggggtgaggg agttacaccc aatccctgac cttaataaac
    15181 tcacttaacg ccgcttctgg gctgccatct tttggctgat aatcatactc ccagcgtacc
    15241 agtggcggca ttgacatcag gatcgactcg gtgcgcccgc cagtttgcag gccaaacaga
    15301 gtgccgcgat cccagaccag attgaactcg acataacgac cgcgacggta gagctgaaaa
    15361 ttgcgctcgc gctcgccgta ggccatcgct ttacgtcgct ctacaattgg taaataagcg
    15421 tcggtgtagc ctttgcctac cgcctgcata aaggcaaaac agtggtcgaa atctggcgtg
    15481 ttcagatcat caaagaacag cccgccaata ccgcgctgtt cgttgcgatg tttgaggtag
    15541 aagtaatcgt cgcaccactt tttgtaacgg ggataaacgt cttcaccaaa tggcaggcac
    15601 aggtcacggg cggtgcggtg ccagtgaatg gcgtcttctt caaaaccata gaaaggggtt
    15661 aaatcgaagc cgccgccaaa ccaccagacg ggctctgcac ccggtttttc ggcaataaaa
    15721 aaacgcacat tcgcgtggct ggtgggaaca tacgggttat gcggatgcac taccagtgaa
    15781 acgcccatcg cctcgaaact gcgcccggca agttccgggc gatgagcggt ggcggaagca
    15841 ggcatcgcct cgccgtggac gtgagaaaag ttgacgcctg cctgttcgaa aacaccacca
    15901 ttacgcaaca cccgactacg cccgccgccg ccagcttcgc gctgccaact atcttcgaca
    15961 aattctgcgc catcgacggc ggtcagctgc tgacaaatcg tatcctgaag gttgagcaga
    16021 aactgtttaa cctggtgtgc gtcgggtttc ataacttatc gctttttcga atgtgctttc
    16081 tggttgtcga accagtggaa ataactgatc acgccttcag caatcgctgt ggcgattttc
    16141 tgacgaaacg ccaccgtgcc taacagccgc tcttcttccg ggttggtgat aaacgaggtt
    16201 tccaccagca ccgaaggaac cgacggcgat ttcaacacca caaacgcggc ttgttcggtg
    16261 ttgcggctgt gcagtttatg caccggctta atctttttca gaatatgcga gccaagcgtc
    16321 aggctgtttt taatagtatc ggtttgcacc agatcaaaca gcacttgctg caataggtga
    16381 tccttgtcag tcgccttttt accggcaact tcatcggcgc ggttttcgcg ttcagacagg
    16441 tatttcgcca ttgcgctact tgccccacgg ttagagaggg caaataccga agcaccggca
    16501 gctttcgggt tggtaaagcc atcggcatga attgacataa acagatctgc gccatgttta
    16561 tgggcgattt caacgcgatc gtaaagtggg ataaacgtat cgccagaacg cgttaaacgc
    16621 gcatcaatcc catgattacg caaaatggaa cggacgtttt tagcaatcgc cagcaccaca
    16681 tgtttttctt tcgaaccgtt gcgtccgatc gctccggtat caattccgcc gtggcctgga
    16741 tcgagaacaa cgacacgttt gccgccagat tttttggctt tcggcttgct gtgtccgttg
    16801 ctggttttta aaggttcgtc tttggcgatg gcttgcgaca ttcctgacaa cgtcagggca
    16861 gccaaaccgg ctttcagcac ctggcggcgc gaagtgagtg tttttagtgg tttaaaagtg
    16921 ctcatacggc ctgagttgta ataataaagt tccagatgtt atatcgtatc gcgtaatccg
    16981 ttacgacggt ttgatgttga gaattgtttt acttttcatt tcaatagttg acaactcccc
    17041 attatgccac tttttcaacc gattttcgtc agatattggc taattcgacc gttcacgcca
    17101 taatcagcct tttaacccgc aaaaaggtaa cgaataccat ggagatacgc gtatttcgcc
    17161 aggaagattt cgaagaggtc atcacccttt gggagcgttg cgacttgctg cgtccgtgga
    17221 acgatccgga aatggacatc gagcgtaaga tgaaccatga cgtcagtttg tttttggtcg
    17281 ctgaggtaaa cggtgaagtg gtcggaacgg tgatgggcgg ttatgacggg catcgcgggt
    17341 ctgcttatta tcttggcgtg catccagagt ttcgtgggcg tgggattgcc aatgcgttgc
    17401 ttaatcggct ggagaaaaag ctgattgctc gtggctgccc gaaaattcag atcaatgtgc
    17461 cggaagataa cgacatggtg ctcggaatgt atgaacgcct gggatatgaa cacgccgacg
    17521 tgctgagtct gggtaagcgt ttgattgaag atgaagagta ctgagttcca tcctgtccat
    17581 tatgatgcgc acggacgcct gcgtttaccc ttgctcttct ggcttgtgct attgcttcag
    17641 gcgcgaacct gggtgctgtt tgtcattgcc ggtgcgtcgc gcgagcaggg tactgcgctg
    17701 ctaaatctgt tttatcccga tcacgataat ttctggctgg ggctgattcc aggcattccg
    17761 gcggtgctgg cgtttttgtt gagcggtcgg cgggctacat ttcctcgcac ttggcatgtg
    17821 ctctattttc tgttgctgtt ggcgcaggtg gttttacttt gctggcaacc gtggctgtgg
    17881 ctgaacggtg aatccgttag cggtatcggt ctggcgctgg tagtggcgga tattgtggcg
    17941 ttaatctggc tgctgaccaa tcgacgttta cgcgcttgtt ttaatgaaga aaaagaataa
    18001 cggcactttt tggtgaattt gcactccaag caacgttatt gaataaccaa aggaagtgac
    18061 atgaaatcgc tgcgtttaat gttatgcgct atgccgttga tgctgaccgg ctgttccacg
    18121 atgtcgtcag ttaactggtc ggccgctaac ccgtggaact ggtttggatc gtccaccaaa
    18181 gtgagcgagc agggcgtggg tgaattaacg gcgtccacac cactgcaaga acaagccatt
    18241 gctgatgcgc ttgatggcga ttatcgcctg cgcagcggaa tgaaaaccgc gaacggcaac
    18301 gtggtgcgct tttttgaagt gatgaaaggc gacaacgtgg cgatggtgat taacggcgat
    18361 caaggcacga tcagtcgcat tgacgtgctg gatagcgata ttcctgctga caccggtgtt
    18421 aaaatcggta caccgtttag cgacctttac agcaaagcat ttggcaattg ccaaaaagct
    18481 gatggtgatg ataatcgtgc cgtcgaatgt aaagccgaag gcagtcaaca tattagctac
    18541 cagttcagcg gggaatggcg tggtcctgaa gggttaatgc cttcggacga taccctgaaa
    18601 aactggaaag tcagtaaaat tatctggcgg cgttaatttg cgtctgaaca aaccgccgcc
    18661 gcgaaaaaac acgtaaaata gcgctcaaca atgccacgga ttgcgtggca ttcttatttt
    18721 caggaggaac aatgtctcag gttcagagtg gcattttgcc agaacattgc cgcgcggcga
    18781 tttggatcga agccaacgtg aaaggggaag ttgacgccct gcgtgcggcc agtaaaacat
    18841 ttgccgacaa actggcaact tttgaagcga aattcccgga cgcgcatctt ggtgcggtgg
    18901 ttgcctttgg taacaacacc tggcgcgctc tgagcggcgg cgttggggca gaagagctga
    18961 aagattttcc gggctacggt aaaggccttg cgccgaccac ccagttcgat gtgttgatcc
    19021 acattctttc tctgcgtcac gacgtaaact tctctgtcgc ccaggcggcg atggaagcct
    19081 ttggtgactg cattgaagtg aaagaagaga tccacggctt ccgttgggtg gaagagcgtg
    19141 acctgagcgg ctttgttgac ggtacagaaa acccggcggg tgaagagacg cgtcgcgaag
    19201 tggcggttat caaagacggc gtggatgccg gcggcagcta tgtgtttgtg cagcgttggg
    19261 agcacaacct gaagcagctc aaccggatga gcgttcacga tcaggagatg atgatcgggc
    19321 gcaccaaaga ggccaacgaa gagattgacg gcgacgaacg tccggaaacc tctcacctca
    19381 cccgcgttga tctgaaagaa gatggcaaag ggctgaagat tgttcgccag agcctgccgt
    19441 acggcaccgc cagtggcact cacggtctgt acttctgcgc ctactgcgcg cgtctgcata
    19501 acattgagca gcaactgctg agcatgtttg gcgataccga tggtaagcgt gatgcgatgt
    19561 tgcgtttcac caaaccggta accggcggct attacttcgc gccgtcgctg gacaagttaa
    19621 tggcgttgta agcgtttctg attcgatgat taaaggccag ccagtaacaa aactgactgg
    19681 cctttttatt acttttgctt caacgccgca tacacctgat caactaccca accataagtt
    19741 gcaatcggta actgaccgct ttcgaacata ttgggatttt tttgtggatc ggcaactggc
    19801 gaatggggct tgttgcttaa catcacaatc gccatatgat tgaccggatc gataacggtt
    19861 actgtcccgg tccagccagt gtgaccgtag gtttgcgggc ttgccagcgt gccaaacgtc
    19921 ggcgtcatgg tggcattacc attcacgcgc cagccgaggc caaaagtggc atcttcctta
    19981 gagctggtgg tgaacatctt caccgtttcc gcactgaaca gctgcacatc gccatagccc
    20041 ccgccgttca gcatcgtttg cattaacacc gcaatatcgc cggtattgga aaacaaacct
    20101 gcgtgcccgg aaacgccgcc catcgaataa aaggcttttt catcgtgcac ctgaccccag
    20161 agagtggagg tgcggatatt cgggaaatgg atcacgccat cgcgggtatt gccgttcaat
    20221 tccgtggcgg caatttgttg cggtttaaag cctttcagca gcgggttaaa taccgtatgt
    20281 gtcaggccga gcgggcgata aatcgactct tcaacatagc ggtcaagcgg ctgaccggta
    20341 accgactcga cgataaatcc aagcagcata taatcgacat cgctgtagat atgtttgctg
    20401 ccgggctgat attccagcgg cgtgcgcttg atcatctcca gcgtctggcc tttatcctgg
    20461 gaatataacg cgcccgcgac cgctttattc gggtattgcg gatctgccgg gaaaccgccg
    20521 ctgtgatgca gcaggtcaga aatccgcagg gtgtttttgc ctttaatggt gtcatttgga
    20581 ctatcggcaa accccggaat atatttcgca atccgatcgt caggatgcag tttgccttcg
    20641 ctcatcagct tttgcagggc gaagttcgtg gcgtacattt tggtgtttga ggccaggtca
    20701 tacagcgtcc cggtggtggc tttgacaggc tgttccatca acacgctgcc atcgtacttt
    20761 ttcgccgctc cccaggcttt gcgatacaca atctgattat ctttaatgat cagcaggttt
    20821 acgccgggat aaccgacatc aatttgctgg ctaatccagc gatccatctg gttaagccgt
    20881 tcgacgttaa accctgcttt ctctggcgag ctttctgtca gaacagggta ttttgcggcg
    20941 ctgacgctac aactaacagc cagcagagaa agatagagca ttgtccgttt catgatatca
    21001 gtccagattg acgttacggc agccaaagag cgtggtgaaa ataaacccgc aaacccatgc
    21061 caccagaatc ccgccagcat aaaccgccat ggccggaagg atgccttgtg cggaagtcat
    21121 cagcggcagc gctaccagac cagacggccc aaaggcgctg tttaagccca tcggcagacc
    21181 ccatcaggct atcaggccga taaacaaacc gcccgccgcg ccgcctaaac aggcggtaac
    21241 aaacggcttc atgcggggca gggtgacacc gtaaatcagc ggttcaccaa cgcccagcag
    21301 gccgggaata atcgccccgc gtacctgact gcgtagcgca ctgtgcggtt gcgcccgcca
    21361 gtagagtgcc agcgccgcgc ccacctggcc cgcgcctgcc attgaaagga tgggaaataa
    21421 gctgttgaat ccctagctgt ccattaacgc gaggtaaaca ggaataaagc cctgatgcac
    21481 gccaaatacc acggcgatca ggaacagccc ggctaaaacc gcacaaccga acggattact
    21541 gttcaggtgc ataaacagcc atgacatacc ttcgaataac cagccaccca gcggcataat
    21601 gatcaggtac gcaagcgtag cggtgatcag cagggtgatt aacgaggtca gcagcatgtc
    21661 gagatcgtcc gacataaagc ggcgcaccat gccttcaatg cgggcgcagg cccacgcggc
    21721 aatcagcacg ccgataatat tgccgcgcgg atcgatgggc agaccaaaga aatcgtgaaa
    21781 accggcgtag tcaccggtgg ttgcagcggg gttataaccg agcaaaaaga gtgcggcaat
    21841 aattgcgcca ttgacgcccg tgccgccaaa tgcctgggcg gcgttatagc ccaccagaat
    21901 caccaggaaa gtgaacaaac ctttgctgaa caccttcata aaattcagcg cgtcgggtag
    21961 cgtgccctga gcatctgccg gaacgtgcat caccgtggca attaacgtcg ctatccccag
    22021 cagcagaccg gcggcaataa aaccggggat cagcggcgtg aagatggtgg cgaatttggc
    22081 gagaaattgt tgcacgccgg aggtttgttt ggcttttaac tgacgtttgt tctgggcggc
    22141 gatttctgcg gcatcctgta ccggtgcctc gcccagcagc tcgctcatcg cttttgcggc
    22201 acgatgcgct ttaccaggcc caaaaacgac ctgcacctgg tcgctggtca aaatgacgcc
    22261 cttcacgcct tccagcgttt tgatattggg atcaaccagt gaactgtcat gtacacccag
    22321 acgcaggtgc gtcatacagt taccacaact ggcgatattt cccagtccgc cgacacgggt
    22381 aagaatggtg ttcagaagtt cactgctgat ctctttggcc atgggttatt ccttgtctaa
    22441 aacctgacga ataaagccgc cgtgttgatc caggcgtttt ttagcttctg cggcatcgag
    22501 atttttcagc accatcacaa tggccgtttt acagttgcgc tcgcaggcaa ttaacgccgc
    22561 ttccgcttgt tcggcattac atccggtggc gtttttaacg atattgacct ggcgcacatg
    22621 cagtttttcg ttggtggcga ccacatcgac catcaggttg ccgaacactt tgccggattt
    22681 aatcatcagc ccggtggaaa gcatattgag caccagtttc tgcgctgtgc ctgctttcat
    22741 tcgcgaagaa ccagtgacca cttcggcacc tacaatcggt gtaatggcaa actcggcggt
    22801 ggtggaaacg gcactccccg gattacagga aattcccact gtgcggcaac cgagctggcg
    22861 ggcgtattcc agtccggcaa tcacatacgg cgtacgaccg ctggcggcaa taccgaccac
    22921 cacatcctgt gccgttaaat ttatattttt cagatcgtta atgccgcctt ctcggctatc
    22981 ttccgtgcct tccaccgcgt gctgaatggc atattcgccg ccagcaatca aaccaaccac
    23041 cagacccggt ttcacgccgt aggtgggcgg acattcgctg gcatccagaa tccccagacg
    23101 accggatgtt cccgcaccga gataaatcag acgaccgccg ccgcttacct gggcgtggat
    23161 aacatcgatc gccgcggcga tatccggcag gacgcgctca acggcaagcg gtacggtttt
    23221 atcttcatcg ttgataatcc ggcacatttc cagcgtcgat acgcggtcaa tttcagccga
    23281 ggcggtgttc gagccttcag taatcatctt ttcaagttgc atcggtggtg accttactat
    23341 ttacgatcaa ataattgcag atgtatggaa tcatatatcc cgaattgaca gacttaaagg
    23401 aatcattgag tctacacttg cagagtgtga agagcgtcat aagaacttaa gcggaatttt
    23461 tgctgccagc ccgggagagt ggaagatgtt gtacctgacg aaaatacgca atgcggaaag
    23521 tgaatttacc gagaacgagc aaaaaattgc tgactttctg cgggccaacg tgagtgagct
    23581 gaaatccgtc tcttcgcgca agatggcgaa acagctcggc atcagtcagt cgagcattgt
    23641 gaaatttgcc cagaagctcg gcgcgcaagg atttactgaa ttgcgtatgg cgttaattgg
    23701 cgaatacagt gccagccggg agaaaacgaa tgccacggcg ctgcacctgc acagttcgat
    23761 taccagtgat gattcgctgg aagtgattgc ccgcaaactg aatcgcgaaa aagagctggc
    23821 gctggagcaa acctgtgcgt tgttcgacta cgctcggctg caaaaaatca ttgaggtcat
    23881 cagtaaagca ccgtttatcc agataacggg cctgggcggg tcggcgctgg tggggcgcga
    23941 tctgtcgttc aaactgatga aaattggcta tcgcgtggcc tatgaagccg acacacacgt
    24001 acaagccacc gtttcccagg cgttgaaaaa aggcgatgta caaatcgcta tctcttacag
    24061 cggcagtaaa aaagagattg tcttgtgcgc agaagcggca cgaaagcagg gggcaacggt
    24121 gattgccatt acttctctgg ctgactctcc gctgcggcgg ctggcgcatt tcacgctcga
    24181 taccgtttcc ggtgaaaccg aatggcgtag ctcgtcgatg tctacccgca cggcgcaaaa
    24241 ttctgttacc gatttactgt ttgttggtct tgtgcaactc aatgatgtgg agtcattgaa
    24301 aatgattcag cgcagcagtg agctaacaca acgcctgaaa tgatgcataa agcagcgact
    24361 ggattgagat tttcctgaat tagtgagctg atccgcagca atattttgtt tatcctgtat
    24421 tttcagaggg aatggagtgt aacgctctgt attaacaagg agagcattaa aatgggtaaa
    24481 ctcacgggca agacagcact gattacgggc gcattgcagg gaattggcga aggaattgcc
    24541 agaacttttg cgcgtcatgg cgcaaaccta atcttgctgg atatctcccc tgagatcgaa
    24601 aagctggcgg acgaactgtg tggtcgtggt catcgctgta cggcggttgt cgccgatgtg
    24661 cgtgacccgg cgtcggtagc cgcagctatc aaacgcgcga aggaaaaaga agggcgcatt
    24721 gatatcctgg tgaataacgc aggcgtttgt cgtctgggca gtttcctcga tatgagcgat
    24781 gacgatcgcg atttccatat tgacatcaat attaaaggcg tatggaacgt cacgaaggcg
    24841 gtgctgccgg agatgattgc ccgcaaagat ggtcgcattg tgatgatgtc ttcagtcact
    24901 ggtgatatgg tggccgatcc tggcgaaacg gcgtacgcct taacgaaagc ggcgattgtt
    24961 ggcctgacaa aatcgctggc ggtggagtac gcgcagtctg gtattcgcgt taacgccatt
    25021 tgcccgggat acgtgcgcac accaatggcg gaaagcattg cccgccagtc gaacccggaa
    25081 gatccagagt cggtgctgac tgaaatggcg aaagcaatcc cgctgcgtcg cctcgccgat
    25141 ccgctggaag tcggcgaact ggcggccttc ctcgcatcgg atgaatccag ctatttaacc
    25201 ggtacacaga atgtgattga tggcggcagc acactgccgg agacggttag cgtcggtatc
    25261 tgattcacct ctgtttcctc cctgcatttg tggggaggat ttcgttttaa acccggttct
    25321 tattcccttt tcaacttcca aatcaccaaa cggtatataa aaccgttact cctttcacgt
    25381 ccgttataaa tatgatggct attagaaagt cattaaattt ataagggtgc gcaatggccg
    25441 ttaacttact gaaaaagaac tcactcgcgc tggtcgcttc tctgctgctg gcgggccatg
    25501 tacaggcaac ggaactgctg aacagttctt atgacgtctc ccgcgagctg tttgccgccc
    25561 tgaacccgcc gtttgagcaa caatggacca aagaaaacgg cggcgacaaa ctgacaataa
    25621 aacaatctca tgccgggtca tcaaaacagg cgctggcaat tttgcagggc ttaaaagcag
    25681 acgttgtcac ttataaccag gtgactgacg tacaaatcct gcatgacaaa ggcaagctga
    25741 tcccggcgga ctggcaaacg cgcctgccaa ataatagctc gccattctac tccaccatgg
    25801 gcttcctggt gcgtaagggc aacccgaaga atatccacga ctggaacgac ctggtgcgct
    25861 ccgatgtgaa gctgattttc ccgaacccga aaacgtcggg taacgcgcgt tacacctatc
    25921 tggcggcatg gggcgcagcg gataaagctg acggtggcga taaagccaaa accgaacagt
    25981 ttatgaccca gttcctgaaa aacgttgaag tgttcgatac tggcggtcgt ggcgcgacca
    26041 ccacttttgc cgagcgcggc ctgggcgatg tgctgatcag cttcgagtcg gaagtgaaca
    26101 acatccgtaa acagtatgaa gcgcagggtt ttgaagtggt gattccgaaa accaacattc
    26161 tggcggaatt cccggtggcg tgggttgata aaaacgtgca ggccaacggt acggaaaaag
    26221 ccgccaaagc ctaccttaac tggctctaca gcccgcaggc gcaaaccatc atcaccgact
    26281 attactaccg cgtgaataac ccggaagtca tggataaact gaaagacaaa ttcccgcaga
    26341 ccgagctgtt ccgcgtggaa gacaaatttg gctcctggcc ggaagtgatg aaaacccact
    26401 tcaccagcgg cggcgagtta gacaagctgt tagcggcggg gcgtaattaa tgtttgcagt
    26461 ttcatcaaga cgcgtgctgc cgggctttac cttaagcctc ggcaccagtc tgctgtttgt
    26521 gtgcctgatt ttgctgctgc cgctctccgc gctggtgatg cagctggctc agatgagctg
    26581 ggcgcagtac tgggaggtga tcaccaaccc gcaggtagta gcggcctaca aagtaacgct
    26641 gctgtcagcg tttgtggcat cgatttttaa cggcgttttt ggcctgctga tggcgtggat
    26701 cttaacccgc tatcgctttc cgggccgcac gctgcttgat gcgctgatgg atttaccctt
    26761 tgcgctgcca acggcagtcg ccggtttaac gctagcctcg ctcttttctg taaacggttt
    26821 ttacggtgaa tggctggcga agtttgatat caaagtcacc tatacctggc tggggattgc
    26881 ggtggcgatg gcctttacca gcattccgtt tgtggtacgt accgtgcagc cggtgctgga
    26941 agagttaggc ccggaatatg aagaagcggc agaaacgctt ggcgcaacgc gctggcagag
    27001 tttctgcaaa gtggtgctgc cggaactttc tccggcgctg gtggcgggcg tggcgctgtc
    27061 gtttacccgt agtcttggtg aatttggcgc ggtgattttt atcgccggaa atatcgcgtg
    27121 gaagacggaa gtgacgtcgc tgatgatttt tgtgcgctta caggagtttg attacccggc
    27181 agcgagcgcg attgcttcgg tgatcctcgc ggcatctctg ctgctgctgt tctcaattaa
    27241 cactctgcaa agtcgctttg gtcggcgtgt ggtaggtcat taatggcgga agttacccaa
    27301 ttgaagcgtt atgacgcgcg cccgattaac tggggcaaat ggtttctgat tggcatcggg
    27361 atgctggtat cggcgttcat cctgctggtg ccgatgattt atatcttcgt acaggcgttc
    27421 agcaaagggc tgatgccggt tttacagaat ctggccgatc cggacatgct gcacgccatc
    27481 tggctgacgg tgatgatcgc gctgattgcc gtaccggtaa acctggtgtt cggcattctg
    27541 ctggcctggc tggtgacgcg ctttaacttc cctggacgcc agttactgct gacgctactg
    27601 gacattccgt ttgccgtatc gccggtggtt gccggtctgg tgtatctgct gttctacggc
    27661 tctaacggcc cgctgggcgg ctggctggat gaacataatc tgcaaattat gttctcctgg
    27721 cccggaatgg tgctggtcac catctttgtg acgtgtccgt ttgtggtgcg cgagctggtg
    27781 ccggtgatgt taagccaggg cagccaggaa gacgaagcgg cgattttgct tggcgcgtct
    27841 ggctggcaga tgtttcgccg cgtcacattg ccgaacattc gctgggcgct gctttatggc
    27901 gtggtgttga ccaacgcccg cgcaattggc gagtttggcg cggtgtcggt ggtttccggc
    27961 tcgattcgcg gcgaaaccct gtcgctgccg ttacagattg aattgctgga gcaggactac
    28021 aacaccgtcg gctcctttac cgctgcggca ctgttaacgc tgatggcgat tatcaccctg
    28081 tttttaaaaa gtatgttgca gtggcgcctg gagaatcagg aaaaacgcgc acagcaggag
    28141 gaacatcatg agcattgaga ttgccaatat taagaagtcg tttggtcgca cccaggtgct
    28201 gaacgatatc tcactggata ttccttcagg tcagatggtc gcgttgctgg ggccgtccgg
    28261 ttccgggaaa accacgctgc tgcgcattat cgccgggctg gagcatcaaa ccagcgggca
    28321 tattcgcttc cacggcaccg acgtcagccg cctgcacgcg cgcgatcgca aggtcggttt
    28381 cgtgttccag cattacgcgc tgttccgcca tatgacggta ttcgacaata tcgcttttgg
    28441 cctgacggtg ctgccgcgtc gcgatcgccc gaatgccgca gccatcaaag cgaaagtgac
    28501 aaaattgctg gagatggtgc agcttgctca tctggcggat cgttatccgg cacagctttc
    28561 cggcggccag aaacagcgcg tggcgctggc gcgcgcgctg gctgtagaac cgcaaattct
    28621 gctgcttgat gaaccgtttg gcgcgctgga tgcgcaggtg cgtaaagagc tgcgtcgctg
    28681 gctgcgtcaa ctgcatgaag agctgaaatt caccagcgtg ttcgtgaccc acgaccagga
    28741 agaagcgacg gaagtagctg atcgtgtagt ggtgatgagc cagggcaata ttgaacaggc
    28801 tgatgcgccg gagcaggtat ggcgcgaacc agcgacccgt tttgtgctcg aatttatggg
    28861 cgaagtgaac cgcctgcagg gaaccattcg cggcgggcag ttccatgttg gcgcacatcg
    28921 ctggccgctg ggctacacac ctgcttatca ggggccggtg gatctcttcc tgcgcccgtg
    28981 ggaagtggat atcagccgcc gtaccagtct cgattcgccg ctgccggtac aggtactgga
    29041 agccagcccg aaaggtcact acacccaatt agtggtgcag ccgctggggt ggtacaacga
    29101 accgctgacg gtcgtgatgc atggcgacga tgccccgcag cgtggcgagc gtttattcgt
    29161 tggtctgcaa catgcgcggc tgtataacgg cgacgagcgt atcgaaaccc gcgatgagga
    29221 acttgctctc gcacaaagcg cctgataggt tgagtgaatg ttaaacgccc ggaggcgctt
    29281 cccgcgatcc gggcttttta atggcaaggt ttgtaacctg tagacctgat aagacgcgca
    29341 agcgtcgcat caggcaacac cacgtatgga tagagatcgt gagtacatta gaacaaacaa
    29401 taggcaatac gcctctggtg aagttgcagc gaatggggcc ggataacggc agtgaagtgt
    29461 ggttaaaact ggaaggcaat aacccggcag gttcggtgaa agatcgtgcg gcactttcga
    29521 tgatcgtcga ggcggaaaag cgcggggaaa ttaaaccggg tgatgtctta atcgaagcca
    29581 ccagtggtaa caccggcatt gcgctggcaa tgattgccgc gctgaaaggc tatcgcatga
    29641 aattgctgat gcccgacaac atgagccagg aacgccgtgc ggcgatgcgt gcttatggtg
    29701 cggaactgat tcttgtcacc aaagagcagg gcatggaagg tgcgcgcgat ctggcgctgg
    29761 agatggcgaa tcgtggcgaa ggaaagctgc tcgatcagtt caataatccc gataaccctt
    29821 acgcccatta caccactacc gggccggaaa tctggcagca aaccggcggg cgcattaccc
    29881 attttgtctc cagcatggga acaaccggca ctatcaccgg cgtttcacgc tttatgcgcg
    29941 aacaatccaa accagtaacg attgttggcc tgcagccgga agaaggcagc agtattcccg
    30001 gcattcgccg ctggcctgcg gaatatctgc cggggatttt caacgcttct ctggtggatg
    30061 aggtgctgga tattcatcag cgcgatgcag aaaacaccat gcgcgaactg gcagtgcggg
    30121 aaggaatatt ctgtggcgtc agctccggcg gcgcggttgc cggagcactg cgggtggcaa
    30181 aagctaaccc tggtgcggtg gtggtagcga tcatctgcga tcgtggcgat cgctaccttt
    30241 ccaccggggt gtttggggaa gagcatttta gtcagggggc ggggatttaa gattattgcg
    30301 caggcaccgt tttatccgtc tgcgcaatta aggcatgtaa aaaaggctgt gcgttctcat
    30361 cgctcttacc ggggatcttc acaatatacg gcttcccggt aatcgatgac gacgaagcaa
    30421 ccttatcaat aaactgctcg gcagtgtcaa tgcggttacg ggtattgccg agcttcagac
    30481 gcagatgaga aaccgcttca tcgcaggtat gttcatcacc gttgcgcaca aatatcaaat
    30541 cctttttctg tgctaatccc tccagcatgg cgttgatgcg ggcttcttca tgaacagtta
    30601 acttcgcgta agcggggagt gtcattagta gtgtaatgac cagacaaatg attttcttca
    30661 ttgaattacc ttatccttgt tttgtgatag ccgatttgac tgccagaatc aggtaaagtt
    30721 tcggttggaa tacgatgagg atcatcggca aggagaaaaa attaaatgag tagtttgttg
    30781 ttgtttaacg ataagagtag ggcgctgcag gcggatatcg tcgccgtgca gtcgcaggta
    30841 gtgtacggca gcgtaggcaa cagcattgcg gtgcctgcta taaaacagaa cggcttgaat
    30901 gtctttgccg tgccgacggt attgctgagc aatacgccgc attatgacac tttctacggt
    30961 ggtgcgattc cggacgaatg gtttagcggc tatttgcgtg cgcttcagga gcgtgatgcg
    31021 ctgcgccaac ttcgtgctgt aaccacgggc tatatgggaa cggcatcgca aatcaaaatc
    31081 cttgccgagt ggctgactgc gctacgcaaa gaccatcctg acctattgat catggtcgat
    31141 ccggtgattg gcgatattga tagcggaatt tatgtcaaac ctgaccttcc cgaggcgtat
    31201 cgacaatatt tactgccgct ggcgcaagga attaccccca atatctttga gctggaaatc
    31261 ctgaccggta aaaattgccg cgatctcgac agtgccattg ctgccgcaaa aagtctgctt
    31321 tcagacacat taaaatgggt ggtgattacc agcgcctccg gtaatgaaga aaatcaggag
    31381 atgcaggtgg tggtggtcag tgccgacagc gtgaatgtca tttcccattc acgggtaaaa
    31441 accgacctga aagggactgg cgacctgttt tgtgctcagc ttatcagtgg tttgctgaaa
    31501 gggaaggcgt taaacgatgc ggtgcaccgt gcggggttgc gcgtactgga agtgatgcgc
    31561 tatacccagc agcatgagag cgatgaattg attttgccgc cgctggcgga agcataaaaa
    31621 atggcgccga tgg
//
LOCUS       DAZOXR010000031        30153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZOXR010000031 DAZOXR010000000
VERSION     DAZOXR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.031"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHUNN_001213"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHUNN_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0575640.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHUNN_001214"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHUNN_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0575641.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHUNN_001215"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHUNN_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0575642.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUNN_001216"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUNN_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0575643.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUNN_001217"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUNN_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0575644.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUNN_001218"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUNN_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0575645.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUNN_001219"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUNN_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0575646.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUNN_001220"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUNN_001220"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0575647.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUNN_001221"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUNN_001221"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0575648.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUNN_001222"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUNN_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0575649.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUNN_001223"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUNN_001223"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0575650.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUNN_001224"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUNN_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0575651.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUNN_001225"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUNN_001225"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0575652.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUNN_001226"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUNN_001226"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0575653.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUNN_001227"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUNN_001227"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0575654.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHUNN_001228"
     CDS             complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHUNN_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR0575655.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUNN_001229"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUNN_001229"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0575656.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUNN_001230"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUNN_001230"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0575657.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUNN_001231"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUNN_001231"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0575658.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHUNN_001232"
     CDS             21688..22278
                     /locus_tag="ACHUNN_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0575659.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUNN_001233"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUNN_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0575660.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHUNN_001234"
     CDS             22750..23445
                     /locus_tag="ACHUNN_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0575661.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUNN_001235"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUNN_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0575662.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUNN_001236"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUNN_001236"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0575663.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUNN_001237"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUNN_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0575664.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUNN_001238"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUNN_001238"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0575665.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUNN_001239"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUNN_001239"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0575666.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7058 a         7863 c         8306 g         6926 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaatct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agggtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg acggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagtcgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc tagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac tcaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccaa gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaaatcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaa
//
LOCUS       DAZOXR010000032        30002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZOXR010000032 DAZOXR010000000
VERSION     DAZOXR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.032"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            39..581
                     /locus_tag="ACHUNN_001240"
     CDS             39..581
                     /locus_tag="ACHUNN_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575667.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            664..1365
                     /locus_tag="ACHUNN_001241"
     CDS             664..1365
                     /locus_tag="ACHUNN_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0575668.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1390..3989
                     /locus_tag="ACHUNN_001242"
                     /pseudo
     CDS             1390..3989
                     /locus_tag="ACHUNN_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3980..5050
                     /locus_tag="ACHUNN_001243"
     CDS             3980..5050
                     /locus_tag="ACHUNN_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575669.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5062..5604
                     /locus_tag="ACHUNN_001244"
     CDS             5062..5604
                     /locus_tag="ACHUNN_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575670.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5612..6127
                     /locus_tag="ACHUNN_001245"
     CDS             5612..6127
                     /locus_tag="ACHUNN_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575671.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6093..6829
                     /locus_tag="ACHUNN_001246"
                     /pseudo
     CDS             6093..6829
                     /locus_tag="ACHUNN_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUNN_001247"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUNN_001247"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0575672.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUNN_001248"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUNN_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0575673.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUNN_001249"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUNN_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0575674.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUNN_001250"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUNN_001250"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0575675.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHUNN_001251"
     CDS             12136..14043
                     /locus_tag="ACHUNN_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0575676.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISHLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUNN_001252"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUNN_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0575677.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUNN_001253"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUNN_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0575678.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUNN_001254"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUNN_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0575679.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUNN_001255"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUNN_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0575680.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUNN_001256"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUNN_001256"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0575681.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHUNN_001257"
     CDS             complement(18711..20471)
                     /locus_tag="ACHUNN_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0575682.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHUNN_001258"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHUNN_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0575683.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUNN_001259"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUNN_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0575684.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUNN_001260"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUNN_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0575685.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUNN_001261"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUNN_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR0575686.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUNN_001262"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUNN_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0575687.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHUNN_001263"
     CDS             complement(26072..26518)
                     /locus_tag="ACHUNN_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0575688.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHUNN_001264"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHUNN_001264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0575689.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUNN_001265"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUNN_001265"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0575690.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHUNN_001266"
     CDS             complement(29281..29943)
                     /locus_tag="ACHUNN_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0575691.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7734 a         7084 c         7878 g         7306 t
ORIGIN      
        1 ggcattatca tatttacgaa gagatcagga tgatgacgat gaaaaaaagt gtattgacgg
       61 cgtttataac tgtggtatgt gcaacgtcca gcgttatggc agctgatgat aatgctatca
      121 cggatggcaa agtaacattt aatggtaaag ttattgctcc agcttgtacc ctggtagctg
      181 cgacgaaaga ttcagtagtc acattgccaa atgtcagcgc caccaagttg caaactaatg
      241 gagcagtctc tggcgttaaa actgatgtac caattgcctt agaaggctgt gatgttactg
      301 taactaaaaa tgcgacgttt acctttagtg gtacggctga cggcgtgcaa cctacagcat
      361 ttgccaacca ggctaccacc gatgcagcga cgaatgttgc tctacaaatg tatctgccag
      421 atggttctac atcggtaact cccggtaccg aaacgtcgaa tattcagtta gcggattctg
      481 ctgagcaaac agtgaccttt aaagttgatt atatcgctac ggggaaagcg acttctggta
      541 atgtgaatgc ggtaacaaat ttccatatta actattatta atagaactca ttaattgttt
      601 tattaattag tacccctcta gtgttctgga ggggatattc atatttttta agagtgacta
      661 tttatgaaaa cttgcataac aaagggaatt gtgaccgtaa gtttaacggc aatattactc
      721 agctgctcat ccgcatgggc ggcaggtaaa ggcgggattg gacttgcagc aacacgtctt
      781 gtttattcag aaggtgaaga gcaaatttca ctgggtgtac gtaacaccag tccggatgtt
      841 ccttatctta ttcagtcatg ggtgatgacc ccagataata aaaaatcagc agattttatt
      901 attacaccac cgctatttgt gctgaatccg gctaatgaga atctgttacg cattatgtac
      961 attggtgcgc cgttggcgaa agacagagaa acccttttct tcactagcgt acgggcagtc
     1021 ccttcaacaa cgaagcggga agagggaaat accctgaaga ttgccacaca aagcgtcatc
     1081 aaacttttct ggcgaccaaa aggtttagcg tatcccttag gcgaggctcc ggcgaaactg
     1141 cgttgcactt cgtcagctga catggttacg gtcagtaacc caacacctta tttcattacc
     1201 ctgacagacc tgaaaatagg gggaaaggta gttaaaaatc aaatgatttc cccctttgat
     1261 aaataccaat tctctctgcc aaagggggcc aaaaatagca gcgtaacgta tcgaaccatc
     1321 aatgattacg gggcggaaac gccacaactc aactgtaaat cgtaagccgt cttcagttaa
     1381 gagagcgaga tgtatagaac tcatcgacaa cacagcctgt taagctctgg tggagtgcca
     1441 tcgtttattg gtgggctggt ggtgtttgtg tcggcagcgt tcaatgcaca agctgaaacc
     1501 tggttcgatc ctgctttttt caaagatgat ccctcaatgg tggccgattt gtctcgtttc
     1561 gaaaaaggac aaaaaataac gccaggggtt tatcgtgtcg atattgttct gaatcagaca
     1621 attgtagata cgcgcaacgt caattttgtt gagataacgc cagagaaggg gattgccgcc
     1681 tgtttgacga ctgaaagcct ggatgcaatg ggtgttaata ctgatgcgtt tccggctttt
     1741 aaacaactgg acaaacaagc gtgtgtgcca ttggcggaga ttattccgga tgccagcgta
     1801 acgtttaatg tgaataaact ccgtctggaa atttcagtac cgcaaatcgc catcaaaagt
     1861 aatgcccgtg gttatgtccc ccctgaacgt tgggatgaag ggatcaacgc gctattactg
     1921 ggatattcgt ttagcggggc taacagtatt catagcagcg cagacagtga ttctggcgac
     1981 agctattttc tgaatttaaa cagtggcgtt aatttaggcc catggagatt gcgcaacaat
     2041 tcaacatgga gtcgcagtag tggccaaacc gcagaatgga agaatctcag cagctatttg
     2101 cagcgggcgg ttattccact aaaaggcgaa ctgaccgtag gtgatgatta tactgcaggc
     2161 gattttttcg atagtgtcag ctttcgtggt gtgcagctgg cgtcagatga caacatgctg
     2221 ccagacagcc tgaaagggtt tgcgcctgtg gtgcgtggta tcgccaaaag caatgcccag
     2281 ataacgatta agcaaaatgg ttacaccatt taccaaactt atgtatcgcc tggtgctttt
     2341 gaaattagtg atctctattc cacgtcgtcg agcggtgatt tgttagttga aatcaaagaa
     2401 gcggacggca gcgtcaatag ctacagcgta ccgttttcca gcgtgccatt actccagcgt
     2461 caggggcgaa tcaaatacgc ggtgacactg gcgaaataca gaaccaatag taatgaacag
     2521 caggagagca aatttgccta ggccacgttg cagtggggcg gaccgtgggg aacgacatgg
     2581 tatggtggtg gacaatatgc tgaatattac cgtgccgcca tgtttggtct gggatttaac
     2641 cttggcgatt tcggagcaat ttcgttcgat gcgacccagg cgaagagtac gctggcagac
     2701 caaagcgaac ataaaggtca gtcatatcgt tttctgtatg ccaaaacgct caaccaattg
     2761 ggcactaatt ttcaattgat gggctatcgc tattcgacgt cgggtttcta caccctttcc
     2821 gacaccatgt ataaacatat ggatggctac gaatttaatg acggtgatga tgaagatacg
     2881 ccgatgtggt cgcgttatta caatttgttt tacaccaaac gtggcaaact gcaggtcaat
     2941 atctcccagc aattaggcga gtacggttcg ttttatttaa gtggtagcca gcaaacttac
     3001 tggcataccg atcaacagga tcggctatta cagtttggct acaacacgca aattaaagat
     3061 ctctcgctgg ggggttcctg gaactacagt aagtcccgtg gtcaacctga tgctgatcag
     3121 gtgtttgcac taaatttttc cctgccgctc aatctgttgc tccccagaag taatgatagc
     3181 tataccagga aaaaaattac gcctggatga cctctaacac cagcatcgat aacgaagggc
     3241 acattacaca aaacctgggt ttaacggaga cactactcga tgacggtaac ctgagctaca
     3301 gcgtgcaaca gggatataac agcgagggga aaacggctaa tggtagcgcc agcatggact
     3361 acaaaggggc gtttgcagat gcccgagtgg gctacaacta cagcgataac ggcagtcaac
     3421 aacaactgaa ctacgctctt tcaggcagtt tagttgccca ttcacagggc attaccctgg
     3481 ggcaatcgct gggggaaact aacgtgctga ttgcagcacc aggcgcagag aatactcgtg
     3541 tggcgaacag caccgggctg aaaactgact ggcgcggata taccgttgtc ccctatgcca
     3601 cttcttatcg ggaaaatcga atcgcacttg atgcggcatc gttaaaacgt aacgtggatc
     3661 ttgaaaatgc agtagtcaac gtggttccca ccaaaggggc gttggttctg gcggagttca
     3721 atgcccatgc gggagcaagg gtattaatga aaacatcaaa gcagggtata ccgctgcgtt
     3781 ttggcgcgat agcgacgctg gacggcgtac agactaatag cggcattatt gatgatgatg
     3841 gctcgctcta tatgtccggt ttgcccgctc agggagcgat aactgtacgc tggggcgaag
     3901 ctcccgatca aatttgtcat atcagttacc agcttaccga acaacaaatt aactctgcga
     3961 ttacgcgaat ggatgccata tgcagataat ctttggagaa aaatgcgtgt tattactacg
     4021 actatttttt gccgccgtct taatgctatg gtgcgctcaa actgctgctt atagcgggca
     4081 gtgtcatacc actcagggga atccgtatat tggcgtcaat tttggcgtta aaaccctgga
     4141 ggaagaagaa aatacggctg gagtagttaa agacaaattt tatcagtgga acgaatcgaa
     4201 tgattattat gtttcctgtg attgcgataa agacaatgtc agaagtggcc gatgggcatt
     4261 cgccgcggat tcaccgttag tctatttagg cgacaactgg tacaaaatta atgactatct
     4321 tgccgccaaa gttttattgc aggttaaagg cagttctcct actgcggttc ctttcgaaaa
     4381 cgtgggcaca ggggcagata cacgatggca tatttgcgat cccggcggtc aacgtttagg
     4441 tggccagggg gctagcggta atagcggtag cttttccctg aaaatattgc agccgttcgt
     4501 tggttcggtc gtcattcctc ctatggcgct ggcgcgatta tttgaatgct acaacatacc
     4561 cgcaggtgat tcctgcacga ctacaggtac accggtttta gtgtattacc tgtctggtac
     4621 gatcaattca cttggctcat gttccgtcaa tgccggagaa acaatcgagg tcgatctggg
     4681 cgacgcattt gcggctaact ttcgtgttgt agggcataag cctcttgggg ccagaacggc
     4741 agaacttgca attccagtca ggtgtaacac gggaaacgcg gggttagtta acgtcaacct
     4801 gagtctgacg gcaaccacag accccagcta tccccaggcg attaagacgt cacgtcctgg
     4861 cgtgggcgtg gtggtgaccg atagccagaa caacattatt tcccctgctg gtggaacatt
     4921 accgctctct attcctgatg atgcagacag tatcgcgcga atgaatgtct atccagtcag
     4981 cacgacaggt gtaccaccag aaaccgggcg atttgaagcc acggcaacgg tgagaataaa
     5041 ttttgattaa cgcgtgaacg tatgaagaaa gaaacgatat ttcagtgcgt tatcttgttc
     5101 tttagcattc ttaacatcca tgtcgggatg gctgggcctg aacaagttag tatgcatatt
     5161 tatgggaatg tggtcgatca gggctgtgat gtcgccacca aaagtgcatt acaaaatatt
     5221 catattggtg attttaatat cagtgatttt caggccgcga ataccgtaag cactgctgct
     5281 gatttgaata tcgatatcac cggttgtgcc gctagtatta ctggcgcgga cgtccttttt
     5341 agcggcgagg ctgacaccct tacgccgaca ctgctcaaac taactgacac aggcggaagc
     5401 ggtggtatgg caacggggat tgccgtgcaa attcttgatg cgcaaagtca gcaagaaatc
     5461 ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac actcaaatat
     5521 caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac ggcggttctc
     5581 tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct ggattctgtt
     5641 cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg tcaacattag
     5701 cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa tcaatgtgca
     5761 gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg agaaatcttt
     5821 catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgacgtca ccttttcagg
     5881 aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg atgcagccag
     5941 cgggctggca attgcgattc tggatgatgc gaagatattg attccgctta accaggcgag
     6001 taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc aattgcgacc
     6061 tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg tacttcatta
     6121 tgactaatac gtggaacaga ttggcgctct tgattttcgc cgttttatcg ctgctggtgg
     6181 cgggtgaatt gcgggcagga gtggtggtcg gcggaacgcg atttatcttt ccggcagaca
     6241 gagaatcgat atctatttta ctgactaata ccagtcagga atcctggctt attaacagta
     6301 aaatcaaccg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca gcaccattac
     6361 tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg cgcttgctga
     6421 gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta agcattgcca
     6481 gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc tcggtattta
     6541 aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat caacaattac
     6601 gctggacacg gaattccagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cacctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcagatggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgt cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 ca
//
LOCUS       DAZOXR010000033        29893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZOXR010000033 DAZOXR010000000
VERSION     DAZOXR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.033"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            67..729
                     /gene="acrS"
                     /locus_tag="ACHUNN_001267"
     CDS             67..729
                     /gene="acrS"
                     /locus_tag="ACHUNN_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR0575692.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(732..911)
                     /locus_tag="ACHUNN_001268"
     CDS             complement(732..911)
                     /locus_tag="ACHUNN_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0575693.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(995..1879)
                     /gene="yhdJ"
                     /locus_tag="ACHUNN_001269"
     CDS             complement(995..1879)
                     /gene="yhdJ"
                     /locus_tag="ACHUNN_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0575694.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1965..2261)
                     /gene="fis"
                     /locus_tag="ACHUNN_001270"
     CDS             complement(1965..2261)
                     /gene="fis"
                     /locus_tag="ACHUNN_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0575695.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2287..3252)
                     /gene="dusB"
                     /locus_tag="ACHUNN_001271"
     CDS             complement(2287..3252)
                     /gene="dusB"
                     /locus_tag="ACHUNN_001271"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0575696.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3581..4462)
                     /gene="prmA"
                     /locus_tag="ACHUNN_001272"
     CDS             complement(3581..4462)
                     /gene="prmA"
                     /locus_tag="ACHUNN_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0575697.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4474..5925)
                     /gene="panF"
                     /locus_tag="ACHUNN_001273"
     CDS             complement(4474..5925)
                     /gene="panF"
                     /locus_tag="ACHUNN_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0575698.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5915..6157)
                     /locus_tag="ACHUNN_001274"
     CDS             complement(5915..6157)
                     /locus_tag="ACHUNN_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0575699.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6266..7615)
                     /gene="accC"
                     /locus_tag="ACHUNN_001275"
     CDS             complement(6266..7615)
                     /gene="accC"
                     /locus_tag="ACHUNN_001275"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0575700.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7626..8096)
                     /gene="accB"
                     /locus_tag="ACHUNN_001276"
     CDS             complement(7626..8096)
                     /gene="accB"
                     /locus_tag="ACHUNN_001276"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0575701.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9074..10048)
                     /locus_tag="ACHUNN_001277"
     CDS             complement(9074..10048)
                     /locus_tag="ACHUNN_001277"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0575702.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10200..12140
                     /gene="csrD"
                     /locus_tag="ACHUNN_001278"
     CDS             10200..12140
                     /gene="csrD"
                     /locus_tag="ACHUNN_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0575703.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12445..13488
                     /gene="mreB"
                     /locus_tag="ACHUNN_001279"
     CDS             12445..13488
                     /gene="mreB"
                     /locus_tag="ACHUNN_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0575704.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13554..14657
                     /gene="mreC"
                     /locus_tag="ACHUNN_001280"
     CDS             13554..14657
                     /gene="mreC"
                     /locus_tag="ACHUNN_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0575705.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14657..15145
                     /gene="mreD"
                     /locus_tag="ACHUNN_001281"
     CDS             14657..15145
                     /gene="mreD"
                     /locus_tag="ACHUNN_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0575706.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15154..15747
                     /gene="yhdE"
                     /locus_tag="ACHUNN_001282"
     CDS             15154..15747
                     /gene="yhdE"
                     /locus_tag="ACHUNN_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0575707.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15737..17206
                     /gene="rng"
                     /locus_tag="ACHUNN_001283"
     CDS             15737..17206
                     /gene="rng"
                     /locus_tag="ACHUNN_001283"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0575708.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17274..21074
                     /gene="yhdP"
                     /locus_tag="ACHUNN_001284"
     CDS             17274..21074
                     /gene="yhdP"
                     /locus_tag="ACHUNN_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0575709.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21230..22675
                     /gene="tldD"
                     /locus_tag="ACHUNN_001285"
     CDS             21230..22675
                     /gene="tldD"
                     /locus_tag="ACHUNN_001285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0575710.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22803..23668)
                     /locus_tag="ACHUNN_001286"
                     /pseudo
     CDS             complement(22803..23668)
                     /locus_tag="ACHUNN_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            23851..24054
                     /gene="aaeX"
                     /locus_tag="ACHUNN_001287"
     CDS             23851..24054
                     /gene="aaeX"
                     /locus_tag="ACHUNN_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0575711.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24062..24994
                     /gene="aaeA"
                     /locus_tag="ACHUNN_001288"
     CDS             24062..24994
                     /gene="aaeA"
                     /locus_tag="ACHUNN_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0575712.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25000..26967
                     /gene="aaeB"
                     /locus_tag="ACHUNN_001289"
     CDS             25000..26967
                     /gene="aaeB"
                     /locus_tag="ACHUNN_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0575713.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27059..27331
                     /locus_tag="ACHUNN_001290"
     CDS             27059..27331
                     /locus_tag="ACHUNN_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0575714.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27387..27650)
                     /gene="yhcN"
                     /locus_tag="ACHUNN_001291"
     CDS             complement(27387..27650)
                     /gene="yhcN"
                     /locus_tag="ACHUNN_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0575715.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(28015..28485)
                     /gene="argR"
                     /locus_tag="ACHUNN_001292"
     CDS             complement(28015..28485)
                     /gene="argR"
                     /locus_tag="ACHUNN_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0575716.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28920..29858
                     /gene="mdh"
                     /locus_tag="ACHUNN_001293"
     CDS             28920..29858
                     /gene="mdh"
                     /locus_tag="ACHUNN_001293"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0575717.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         7238 a         7395 c         7900 g         7360 t
ORIGIN      
        1 tgatagatta accttcgcta ttttctcacc ctgtgtcgaa tatatttatt tcctgaataa
       61 ttaatcatgg caaaaagaac caaagccgaa gctctgaaga cccggcaaga actgattgaa
      121 actgccatcg cccagtttgc gcaacatggc gtaagcaaga cgacgttcaa cgacattgcc
      181 gatgccgcta acgttacgcg tggcgctatc tactggcact tcgagaacaa gacccaactg
      241 tttaatgaga tgtggttgca acagccttca ttgcgggagt taatccagga acacttgacg
      301 gctggattag agcatgaccc gtttcaacaa ttgcgtgaaa aattgattgt cggcttgcaa
      361 tatattgcca aaattccccg ccagcaggcg ttgctgaaaa tcttatatca caaatgtgaa
      421 tttaatgatg ggatgctggc cgagggagtg atacgcgaaa agatgggctt taatccgcag
      481 actctccgcg aagtattgca ggcgtgtcag caacaaggtt gtgtagcaaa taacctcgat
      541 ttagatgttg tgatgattat tattgatggt gccttcagcg gaattgttca aaactggtta
      601 atgaatatgg cgggttatga tctttataaa caagcacccg cgctggtcga taacgtatta
      661 agaatgttca tgccagatga aaacataacg aaattaattc atcaaacaaa tgaattaagt
      721 gtcatgtaaa ttcacattct gtcctgaaaa ttcagttcgt cacagttaac cagttttagt
      781 ggataaactg agttcatatt acggcatttg tctgtttctg ttgtcagcag ttcaatccac
      841 tgcatcagta aagtatcaaa cagaaaaacg aagacagcga aaacgacgag ccaccaatac
      901 ttgcgaatca ttacctgagc ctgtgcatgt ttaaccaagt ctggcaggaa atatacacga
      961 aatgaataat ctgaaaacca ggcttacact tcctttactt tgcaatgaga tcggggtcaa
     1021 cttcgcttaa ccggcttcgt tttgacaggt tgccggtctt tctttttttc actttcgcca
     1081 gttcttccgc ggagtaatgc gacgcgacat ccagccgtcg aagccccatt ttgatgtact
     1141 cgctgttgat ctcaatacca atgaattttc gtccgctggc gatggctacg gcaccggtag
     1201 taaagctacc ggcaaacggg tcgagaacga tatcgcctgg gttggaagag gcgagaataa
     1261 tgcgtttcag taaggcttcc ggtttttgcg tcgggtggtt ctcatattca tccatcaaat
     1321 aacgcacgcg cggaaaatcc cagacgttac ccggtacttt ttgatgattg tatggctgtg
     1381 gaggattttt gcgataatcg atcaacgcgc gctgcgatcc ggttttggct tctaccagaa
     1441 tagcatcacc gttgaatgtg tagttctttg cgtctttcac catcatcagg atgggttcgt
     1501 acatggagcc gtagtgtttt ttcgcctgta ctccagaact gtcatatgac cagacgatgc
     1561 gacttttgat ggtaaaaagc ttgcggcact ggagatcgat aaagggcatg ttttccgtac
     1621 tgttcataat gtacatgctg ccctgctttt tcagaacgcg gtggcactct acaatcactt
     1681 caaacagcca gtcgataaac agatcttctt tccaggcttc gatcagacca tcaaaatttt
     1741 taccgatgtt atacggtggg tcggcaaaga tcagatcgac gctttcagta ggtagctttt
     1801 taagttcggc aagggcatca ccgtgaataa tggtcttagc ttcattacca aaccgggtcg
     1861 gttcacatcc tgttctcatg gtcactccct ttgtgacacc tataaaaaag gcgcttcccc
     1921 atgccgagta gcgccttttt aatcaagcat ttagctaacc tgaattagtt catgccgtat
     1981 tttttcaatt ttttacgcag cgtaccacgg ttgatgccca tcatcagcgc agcacgggtc
     2041 tggttaccac gggtgtattg catcaccatg tccaacaggg gctgttctac ttcagccagt
     2101 accagctcat agaggtcatt cacatcctga ccattcagtt gagcaaaata gttcttcagt
     2161 gcctgtttaa ccgagtcacg caggggtttt tgggttacct gatcctgaga gttaacggta
     2221 gaaacggtca gtacgtcaga atttacgcgt tgttcgaaca tagttctgtc agctctttat
     2281 ttctgtttac gcaaaatttt cgaagtatgc ctccaacgcc tccagctgtt cgctggcatc
     2341 ctcaatggcg ttgaatgtgc gccgaaactg gtcatttgga gcgtgttcct ggagatacca
     2401 ggaaacgtgt ttacgtgcaa ttcggtaccc ttttgccgga ccataaaagt catgcagttc
     2461 ccgaacgtgc gcgcaaagca agcgcttaac ctctgccaaa ggcagcgggg gcagcaactc
     2521 cccagtgtcc agataatgct ggatttcccg aaagatccag ggtcttccct gagctgcgcg
     2581 gcctatcatc agggcatccg cccctgtata gtcgagcaca gctctggctt taagcgggtc
     2641 agtaatgtcg ccattcgcga taaccggaat ggaaactttc tgcttaactg cccgaatact
     2701 gtcgtactca gcttctccat tgaacaaaca ggcgcgtgta cggccatgaa tggtcagagc
     2761 ctgaatgcca cagtcttcag ccagttgggc aatctcttcg cagttacggt gttccggtgc
     2821 ccagccggtg cgaatcttca gggtaacagg aacgtccact gcattgacga cctcggtaag
     2881 gatcgattta acgacatccg ggtactgcaa gagggctgaa cctgcgagct tgcgattcac
     2941 ttttttagcc gggcaaccca tattgatatc aataatctgg gcaccgcttt ccacgttaat
     3001 acgtgctgca tctgccattt ctttcggatc gctaccagca atttgcacgg tgcgaatacc
     3061 gggttcatca atgtgcacca tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt
     3121 agaagacatc atctcggata ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa
     3181 aggtctgtct gtaatgccag ccatgggcgc tgcgatcagg cgatttctga gctgatattg
     3241 tccgatgcgc atgagttaag aaatgaccat actgtgactg caaggcggcg tatattacgc
     3301 attttttgca cgagatgaaa ggccaaactt tgaccaatcc tctgagatgg atcaaagaat
     3361 tgcatttaaa atgagcgtgg tgagataatt actcataaaa atcatcatat tagaaaatag
     3421 tgactaaaat ttacactcaa agaaatttga gtaagttctc aatttttctt tatgaatgaa
     3481 aattttggca cgcaaatttg cgtaaataac cggcaaattt acgtgccttt tgtgagcttg
     3541 ctcgcacttc gccccgcgtc accctacggc gatgcgaagg ttaattctta cgaccggtaa
     3601 tgcggcacca ctcttctttt tccacgaccg ggtccagtgc gaagctgtcg acataagctt
     3661 cacaaacgct ctctgcctgg cttgccagaa taccggaaag gcccagcaaa ccgcctgaaa
     3721 ccggcaggac gctgattaac ggtgccagtt cacgtaatgg gcctgcaagg atgttagcga
     3781 ccaccacgtc ggctttcatt tcttctggct gatctttcgg taagtagagt tccagacggt
     3841 cagaaacgcc attacgttcg gcgttgtcgc ggctagcctg aatcgcctgc ggatcgatat
     3901 caataccaat ggcttttgct gcacccagtt tcagcgccgc gatagccaga atgccggaac
     3961 cacagccaaa gtcgatgact attttaccgg ttaaatcgag gctgtcgagc cactgcaggc
     4021 acagagaggt agttggatgg gtacccgtac caaacgccag ccctggatct aacatcacgt
     4081 tgacggcgtt ttcgtccggc acatcacgcc agctagggca gatccacagt cgttcaccaa
     4141 agcgcatcgg gtggaaatta tccatccatt cgcgctccca gtctttatct tccagttgtt
     4201 cgattttatg cgcgaagcct gcgccgagca gcggatggtt ttccagaatc gccaccacgt
     4261 cgttcatatc ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg
     4321 tttcgcccgg cagcggctca aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa
     4381 cggcacccgc ttccatcagc gcatcgctaa gatcttcagc gttcgcgccg gtggtgttca
     4441 gtttcagttg gatccaaggc atggcaaaac tctttattta tcagtagtca aaacggtagc
     4501 ttgcgggacg gatgtaccga aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa
     4561 cgagggcacg atagggtgga agcccaggta ctgaatattc agcgtcgcga gtacggcata
     4621 cagcacgccg ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc gttcccagta
     4681 aagacccagc accagcggcc acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt
     4741 cagccagatg atcatttctg gcggcttcca ggcggcaagc agcagcaacg cgccgagaac
     4801 taacgtaatt accgccgaca tccgcttcag acgcgtctcg ttttgcattt gatccggacg
     4861 gatattcaga tagagatctt taatgatcgt agcggaactt tgtagcaatt gggcgttaat
     4921 tgtcgacatg atcgcagcca taggtgcagc cagaaagatc ccggcagcaa acggtggcag
     4981 cacttttacc attaacgtcg ggatcaccag atccggtacg gtgagatcgg ggatcactgc
     5041 acgacctaac gctccggcta ggtgcatacc gaacatcagg attgcgacta caatcgtacc
     5101 gatgatgatc ccccggtgta ccgctttgct gtctttataa gagatacagc gcaccgcagt
     5161 atgcggcagg ccaatcacgc caaagcacac cagtacccag aacgacgtca taaaggcagg
     5221 cgacagaata tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt
     5281 ctgtactgcg ttgcttaagc cgccagcggc atgtaccacg ccaataagca gcacaacggt
     5341 gccaatcagc atcacaagcc cttgcatggt gtcgttcagc acgctggcgc gaaagccgcc
     5401 aaaggcggta tacaacgcaa tgctgatacc gaaaatcagc agcccggttt cataaggaat
     5461 acccgccgcg gtttccagca ggcgcgcacc gccgataaac tgcacggtca ttgccccaac
     5521 gaacgcaacc agcaaactca aactcgccag ccacaccaga agacgactct ggtagcgggc
     5581 aaacagcatg tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg caaacttctt
     5641 gccgagaata ccgagtgaaa gccagactgc aggaagctga atcatcgcca gcaataccca
     5701 gcccagcccg tatttataag cagctcctgg cccgccgata aacgaactgg cactgatata
     5761 ggtcgcggtg agcgtcatcg ccagcacaat accgcccata gagcggctgc cgaggaaata
     5821 ctcattaagg aaggtgccgg tgctccgttt acgcatcgca taaaccgaga taccgaacac
     5881 caccaccaga taggcgacca gcggtagaat tacttcaagc tgcatcgtca tcctccagtg
     5941 ggatatcgcg atagataaat ttcaccatcg cccagcacag tccaataaac agtagcggcg
     6001 tcaggatgca ggccatctca aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag
     6061 gtaagtaagc ggctactaac caaactgcca gatacaaaag ggtcagcccc agcgcccagc
     6121 gcgcctcttt atgggcctga acaaaacgag tgtccatttt ttgtccctga tgggtgaaga
     6181 aagcgaggat tgtaccttat gggggttgtc gatccccagt aataaaaaag gccggaaaat
     6241 ccggcctttt ggcgcttttg cagtcttatt tttcctgaag accgagtttt ttctccagat
     6301 agtggatgtt agtgccacca tgctggaagt tctcgtcatt catgatgcgg atctgcagat
     6361 caacgttggt tttgataccg tcgatgatca gctcctgcag cgcattcttc atgcgggcaa
     6421 tcgccacgtc acggttttca ccgtagcaaa tcagcttacc gatcattgag tcatagtacg
     6481 gcggtacggt gtagcccgcg tagatatgag actcccaacg tacgccaaaa ccgccaggtg
     6541 cgtggaaacg ggtgattttg cctgggcttg gcaggaaggt gttcggatct tcggcgttga
     6601 tacgacattc caccgcatgg ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt
     6661 gaccggcagc gatacggagc tgttctttga tcaggtcaac gccagtgatc atttcggtaa
     6721 ccgggtgttc tacctgaata cgggtgttca tttcgatgaa atagaactcg ccgttttcga
     6781 acagaaactc gaaagtacct gcaccgcgat agccgatatc aacacacgct ttagcgcaac
     6841 gttcgccgat gtagcgacgc agttccgggg taatgcccgg tgctggcgct tcttcgacca
     6901 ctttctggtg gcggcgctgc atggagcagt cacgttccgc cagatagata gcgttgcccc
     6961 gaccgtcagc cagtacctga atctcgacgt ggcgaggatt ttccaggtat ttctccatgt
     7021 aaaccatatc gttgctgaaa gcagctttcg cttccgcacg ggtcatggag atggattgtg
     7081 ccagttcagc gtcgccgcgc actacgcgca taccgcgacc accgccgccg ccggaggctt
     7141 tgataatcac cggataacca atgcgtttag caatggcacg gtttttatcc atatcgtcgc
     7201 ccaacgggcc gtcagaaccc ggtacgcaag ggacgcccgc ttttttcatc gccgcgattg
     7261 cggatacttt gtcgcccatc aggcgaatgg tttctgcttt cgggccgatg aagataaagc
     7321 cggagcgttc aacctgctcg gcaaagttgg cgttctcgga gaggaagccg taacccggat
     7381 ggattgctac tgcgccggtg atttcagcgg cgctgatgat tgccgggatg ttcagataac
     7441 tttttactga cggagcaggg ccaatacaga ccgtttcatc tgccagtaat acgtgtttta
     7501 gatcgcgatc cgcgctggag tgcacagcga cagtcttgat gcccagttct ttacaggcac
     7561 gaagaatacg caatgcaatc tcgccgcggt tggcaataac aattttatcc agcatgttcg
     7621 cctcgttact cgatgacgac cagcggctcg tcaaattcta ccggttgtcc actttcgacc
     7681 agaattgctt tcacggtacc ggatttgtcc gcttcgatct ggttcatcat tttcatggct
     7741 tcaacgatgc acagggtatc gcccacgttg actttctgac ccacttcgat gaacgctttt
     7801 gcgtccgggc ttggggtgcg gtagaaagta ccaaccatcg gggaacgtac gatgtgacca
     7861 ctgatttccg ctgctgctgg cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta
     7921 gattgagctg gctgctgcat cattggtgca gcgtaagctt gttgcatcac agggaaactt
     7981 gcggcaggag ctgcacggct aatgcgtact gactcttcgc cttcagaaat ttccagttcg
     8041 gagatgcctg attcttcaac cagctcgatc agttttttaa tcttacgaat atccatgagt
     8101 gggttccgta ctctttgttt agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc
     8161 gtatgccccg acagctgtat gcatagcgat aaattccagc aggccgggac gccggtctat
     8221 tttgccccag agtatcaatg ttagacttga cggacattgt gcagcgatct gctgctatcc
     8281 tccggcaaaa aacaaaatat accttcgttg cgccaatgtc accttttccg cagcgcaaaa
     8341 actgcgtcag ggaggacgag gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa
     8401 tactggtctc atcagggaag ataatcaaca gctaacatgt aaataacctt caacaccgtg
     8461 taatttgcaa caagccgcac aattcacgaa attagcgcca tcatcgacgg aacttcttat
     8521 aacgtaaggt gaaaagctgt aaagccagcc ctgcgaagat gaggggctgc aatgagataa
     8581 tcttcacaga ccacaaataa tgtagggatg ccaggatcgc gacaagatag acgacattac
     8641 gcagcggttg ctggggatta cccaattttc actgcatcgt ctggatagat gtgaatgcta
     8701 aagcacgcaa aatcaaccga ctgacaattc tcagcattaa ataggttcga gtggttaatt
     8761 tgtagtcaaa taaaatcaga ttattcaccc catggtacca gaaatcagta acaggttaag
     8821 cgtagtgtcg cccaggcaaa gcaccataaa cctaacaggc gacggaggcg tatcaataat
     8881 ggctgtttag aggagtatca atgcgcatca atttgtggtc agtcgaaatc atgactttcc
     8941 agtttggatg atgttgtaca acttcctgac caaggtgttc gacgaaagct gtccggctaa
     9001 ccgcttttcc cgttaaccga ttgaataatg atgtcaaaat gagttccctc tcttattatt
     9061 ccttgctaaa tggttagtta accttcacca gcgtgcgacc ctggatctgg ttattaatga
     9121 tggcctcggc aaagttcggt gcctctgaca gagatatctc ttttgccgcc tgggtataga
     9181 atgattccgg taaatcggcg accagtcgct gccaggcttg tgcgcggcgt tctggtggcg
     9241 tcattactga atccacccct tgcaaacgga cattacgcag aataaatggc atgaccgtgg
     9301 ttggcagagt aaaaccaccc gccagaccac aggccgccac gcagccgccg taattcattt
     9361 gcgccagcac ttttgccagc actttgtcgc caacagtgtc aattgcccca gcccagactt
     9421 gtttttccag aggacgggat tcggcaaact catcacgagg gagaatacgg ctggcaccta
     9481 aacttttcag atattcatgg gtactttcgc gaccggaaac ggcaacgacc tgataaccca
     9541 acttatgcag cagcgccacg gcggtactgc cgacgccacc actggcaccc gtcacgacaa
     9601 tctccccgtc ctgcgggcga acaccggcat cttccagcgc catcacacac agcatggcgg
     9661 taaaaccggc agtaccgata atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa
     9721 ccagccagtc acctttcact cgcgcctgct ccgccagccc accccagtgg ttttcaccaa
     9781 cgccccagcc agtgagtaac acctcctgac cggcatgaaa acgcggatct tcgctggtgc
     9841 gtacagttcc ggcaaaatcg atcccaggaa tcatcggaaa attacggatg atttttccct
     9901 taccggtaat cgccagcgca tctttatagt tcaggctcga ccagtgaaca tcgaccgtga
     9961 catcgccctc cggcaggcga ctttcgtcca gagtctgtac tgatgcgaga gttttgccgt
    10021 cctgctgttc taaaagtaac gcctgcataa gtggtcctca tgtgcatgat ggattggaaa
    10081 ataatttctg aagattatac tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt
    10141 gcgagataaa tctgatttgc tagtatgccc gcttcctcac tatcggagtt aacacaagga
    10201 tgagattaac gacgaaattt tcggcctttg ttacgctgct caccgggtta acaatttttg
    10261 tgactttgct gggctgttcg ctaagtttct acaacgcaat tcagtataag tttagtcatc
    10321 gcgttcaggc ggtggcgacg gcgattgata cccaccttgt gtcgaatgac ttcagcacat
    10381 taaggccaca aattaccgaa ttaatgatgt cggcagatat cgttcgtgta gacctgctcc
    10441 atggtgataa acaggtttat accctggcca gaaatggtag ttatcgtccg gttggctcca
    10501 gcgatctgtt tcgcgaactg agtgttccgt tgataaagca tccggggatg tcgctgcgtc
    10561 tggtttatca ggatccgatg ggcaactatt tccattcttt aatgactacc gcgccgctca
    10621 cgggggcgat tggctttatc attgttatgc tcttcctggc ggtacgctgg ttacaacggc
    10681 aacttgccgg gcaagaattg ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg
    10741 gctctaatgt gttgggaacc atctatgaat ggccgcccag aaccagcagt gcgctagata
    10801 cgctgcttcg tgaaattcag aacgcacgcg aacaacacag ccgtcttgat acgctgatcc
    10861 gctcttatgc cgcccaggac gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc
    10921 agttagcaac gttactggaa gatcaggaga aagtaggtac ccacgggatc gtgatgatga
    10981 ttcgtctgcc ggatttcaat atgttgagcg acacttgggg gcacagccag gttgaagaac
    11041 agttcttcac tctgactaat ctgctgtcga catttatgat gcgctaccct ggcgcactgc
    11101 tggcgcgtta ccaccgcagt gattttgctg cgcagttacc gcaccggacg ttaaaagagg
    11161 cagagagcat cgccggtcag ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc
    11221 tcgatcgcga cgatatgatc cacattggta tctgcgcctg gcgtagtggt caggataccg
    11281 agcaggtaat ggaacatgca gagtctgcca cgcgtaatgc gggattgcag ggcggcaata
    11341 gctgggctat ttacgatgac tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta
    11401 cgcttatcga gcaaatgctc agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta
    11461 ctcgcgaagg tcaggttcat catcgcgaac tcatgtgccg catcttcgat ggtaatgaag
    11521 aggttagctc ggcggagtat atgccgatgg tcttgcagtt tggcttatcg gaagagtatg
    11581 accgtctgca aatcagccgt cttattccac tattgcgtta ctggccagag gaaaatctgg
    11641 cgattcaggt taccgttgag tcgctgattc gcccgcgttt tcagcgttgg ctgcgcgata
    11701 cgttaatgca atgtgaaaaa tcacaacgaa aacgcataat tattgaactt gcagaggccg
    11761 atgtaggtca acatatcagt cgtttacaac ctgttattcg tttagtgaat gctttagggg
    11821 tacgggtagc cgtcaaccag gctggtttga cgctggtaag caccagttgg atcaaagaac
    11881 ttaatgttga gttactcaag ctccatccgg ggctggtcag aaacattgag aagcgaacgg
    11941 agaaccagct gctggttcaa agcctggtgg aagcctgctc cgggaccagc acccacgttt
    12001 acgccaccgg cgtgcgttcg cgaagcgagt ggcagaccct gattcagcgc ggtgttacag
    12061 gcgggcaagg ggattttttc gcgtcctcac agccacttga tactaacgtg aaaaaatatt
    12121 cacaaagata ctcggtttaa cctgccgttt aatccgtttt cacgtagaat aacgcgcgct
    12181 gcgtctcatg ggagtgtgct tgtctgctcg ccagattgtt gcagcacata tgcagatgaa
    12241 tgaccttacg cggttgcaaa caggcgagga atgctgctga tgcattaagc ctttctggac
    12301 tcaggcagag atttgtaaca aaggaaacga actgcactaa ttttcaccgt agcagatgat
    12361 ttttgcgcct tgtcgctgct gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc
    12421 cagctttcag gattatccct tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac
    12481 ttgtccattg acctgggtac tgcgaatacc ctcatttatg taaaaggaca aggcatcgta
    12541 ttgaatgagc cttccgtggt ggccattcgt caggatcgtg ccggttcacc gaaaagcgta
    12601 gctgcagtag gtcatgacgc gaagcagatg ctgggccgta cgccgggcaa tattgctgcc
    12661 attcgcccaa tgaaagacgg cgttatcgcc gacttcttcg tgactgaaaa aatgctccag
    12721 cacttcatca aacaagtgca cagcaacagc tttatgcgtc caagcccgcg cgttctggtt
    12781 tgtgtaccgg ttggcgcgac ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc
    12841 gctggtgccc gtgaagtctt cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc
    12901 ctgccggttt ctgaagcgac cggttctatg gtggttgata tcggtggtgg taccactgaa
    12961 gttgctgtta tctccttgaa cggcgtggtt tactcctctt ccgtgcgcat tggtggtgat
    13021 cgtttcgacg aagctatcat caactatgtg cgtcgtaatt acggttctct gatcggtgaa
    13081 gccaccgcag aacgtatcaa gcacgaaatc ggttcggctt acccgggcga tgaagtccgt
    13141 gaaatcgaag ttcgtggccg taacctggca gaaggtgttc cacgcggttt taccctgaac
    13201 tccaacgaaa tcctcgaagc actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg
    13261 gttgcactgg aacagtgccc gccggaactg gcttccgaca tctccgagcg cggcatggtg
    13321 ctcaccggtg gtggcgcact gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc
    13381 attccagtag ttgttgctga agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg
    13441 ctggaaatga tcgacatgca cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc
    13501 aggggaaatg tctgtttacc ctgcctggtc tgatacgaga atacgcataa cttatgaagc
    13561 caatttttag ccgtggcccg tcgctacaga ttcgccttat tctggcggtg ctggtggcgc
    13621 tcggcattat tattgccgac agccgcctgg ggacgttcag tcaaatccgt acttatatgg
    13681 ataccgccgt cagtcctttc tactttgttt ccaatgctcc tcgtgaattg ctggatggcg
    13741 tatcgcagac gctggcctcg cgtgaccaac tagaacttga aaaccgggcg ttacgtcagg
    13801 aactgttgct gaaaaacagt gaactgctga tgcttggaca atacaaacag gagaacgcgc
    13861 gtctgcgcga gctgctgggt tccccgctgc gtcaggatga gcagaaaatg gtgactcagg
    13921 ttatctccac ggttaacgat ccttatagcg atcaagttgt tatcgataaa ggtagcgtta
    13981 atggcgttta tgaaggccag ccggtcatca gcgacaaagg tgttgttggt caggtggtgg
    14041 ccgtcgctaa actgaccagt cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa
    14101 tccaggtgct gcgcaacgat atccgcgtaa ttgcagccgg taacggttgt acggatgatt
    14161 tgcagcttga gcatctgcca gcgaatacgg atattcgtgt tggtgatgtg ctggtgacct
    14221 ccggtctggg cggtcgtttc ccggaaggct atccggtcgc ggttgtctct tccgtaaaac
    14281 tcgataccca gcgcgcttat actgtgattc aggcgcgtcc gactgcagga ctgcaacgtt
    14341 tgcgttatct gctgctgctg tggggcgcgg atcgtaacgg cgctaacccg atgacgccgg
    14401 aagaggtgca tcgtgttgct aatgaacgtc tgatgcagat gatgccgcag gtattgcctt
    14461 cgccagacgc gatggggcca aagttacctg aaccggcaac ggggatcgct cagccgactc
    14521 cgcagcaacc gacgacagga aatgcagcta ctgcgcctgc tgcgcccaca cagcctgctg
    14581 ctaatcgctc tccacaaagg gctacgccgc cgcaaagtgg tgctcaaccg cctgcgcgtg
    14641 cgccgggagg gcaatagtgg cgagctatcg tagccaggga cgttgggtaa tctggctctc
    14701 tttcctcatt gcgctgttgc tgcaaatcat gccctggccg gataacctga ttgttttccg
    14761 gccaaactgg gtgttactca tcttgttgta ttggatcctg gccttgcctc atcgcgtaaa
    14821 tgtgggcaca ggttttgtga tgggtgccat actggatctg atcagcggct cgacgcttgg
    14881 cgtacgcgta ttggcgatga gcatcattgc ttacctggtg gcgctgaaat accagctttt
    14941 ccgcaacctc gcattatggc agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga
    15001 tattattgtt ttctgggcag agtttttagt gattaacgtc tctttcagac cggaagtgtt
    15061 ctggagtagt gtagtcaatg gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt
    15121 ccgtcagcag tttgcagtgc aataaaggtt tctatgactt ctctgtattt agcttccggt
    15181 tctccgcgtc gtcaggagtt acttgcgcaa cttggtgtga cctttgaacg tattgttacg
    15241 ggcattgagg agcagcgtca gccgcaggag agcgcgcagc agtatgttgt gcgtctggcg
    15301 cgcgagaaag cacaggcggg tgtcgcgcaa acggcgcagg atctcccggt gctgggtgcg
    15361 gatactatcg ttatcctgaa tggagaagtg ctggagaaac cgcgcgacgc agagcatgcg
    15421 gcgcagatgt tgcgcaaatt atcgggtcag acccatcagg tgatgacggc agtggcgttg
    15481 gccgacagcc agcacattct cgattgcctg gtggtcaccg atgtgacttt cagaacgtta
    15541 acagacgaag acatcgcggg ctatgtcgcc agcggtgaac cgttagataa agcaggtgca
    15601 tacggtattc aggggctggg tggctgtttt gtcaggaaga taaatggcag ctatcacgcc
    15661 gtagtcggct tacctctggt tgaaacgtat gaattattaa gtaattttaa cgcactgcgt
    15721 gagaaaaggg ataaacatga cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg
    15781 agtggcgtat attgatggcg gtattctgca ggaaattcat attgaacgtg aggcgcgacg
    15841 cggaatagta ggcaatatct acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc
    15901 ggcttttgta gatattgggc tggataaagc cgcgtttctt catgcatccg atatcatgcc
    15961 gcacaccgaa tgtgtggcgg gtgaagaaca aaagcaattc acggtgcgcg acatctcgga
    16021 actggttcgt caggggcaag atctgatggt gcaggtggtg aaagatccgc ttggcactaa
    16081 aggtgcgcgc ctgaccaccg atatcacgct gccttctcgc tatctggtct ttatgccagg
    16141 ggcttctcac gttggggttt cccaacgtat tgaaagcgaa tcagaacgtg aacgcctgaa
    16201 aaaagtggtc gcagagtatt gcgacgagca gggcgggttt atcatccgta ccgcagcgga
    16261 aggggttggc gaggctgaac tggcctccga tgccgcttat ctgaaacgcg tctggaccaa
    16321 agttatggag cgtaaaaagc gcccgcagac ccgttatcag ctgtacggcg aactggcgct
    16381 ggctcagcgt gttctgcgtg atttcgccga cgccgaactg gaccgcattc gcgttgactc
    16441 acgcctgact tacgaagcat tacttgagtt cacctcggag tacattcccg agatgaccag
    16501 caagctggag cattacaccg gacgccagcc gattttcgat ctctttgatg tcgaaaacga
    16561 aatccagcga gcgctggaac gcaaagtaga actgaaatcc ggtggttatc tcattatcga
    16621 ccagaccgaa gcgatgacca ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa
    16681 tctggacgac accattttca ataccaatat tgaagcgacg caggctatcg ctcgccagtt
    16741 acggttgcgt aatctgggcg ggattatcat tattgatttc atcgatatga ataatgaaga
    16801 tcaccgccgc cgcgtgctgc actcgctgga gcaggcgttg agcaaagacc gggtgaaaac
    16861 cagcgttaat ggtttttcgg cgctggggct ggtggagatg acgcgtaaac gcacccgcga
    16921 aagcattgag cacgtactgt gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa
    16981 aaccgtggaa acggtatgct atgaaatcat gcgcgagatt gttcgtgtcc accatgctta
    17041 cgactccgac cgtttcctgg tctatgcttc tccggcagta gccgaagcct tgaaaggcga
    17101 agagtcacac tcgctggcgg aagtggaaat tttcgttggc aaacaggtta aagtacaaat
    17161 tgaaccgctc tataaccagg agcagtttga cgtcgtaatg atgtaaacag atgctgggcc
    17221 gccatccggc aaagggtttt tgagtcacat ttttagcaga caaggagtga cgggtgaggc
    17281 gattgccggg gattttactg cttactggag ccgcgctcgt tgtgatcgct gccctgctgg
    17341 ttagcggcct gcgtattgct ttaccgcatc ttgacgcctg gcgtccggaa atcctcaaca
    17401 aaatagaatc cgcgactggc atgccggtag aagccagtca gctctcagcc agctggcaga
    17461 attttggccc gacgcttgaa gcacacgaca tccgtgcaga actaaaagat ggcggcgaat
    17521 tttcggttaa acgcgttact ctggcgctgg atgtctggca gagcctgtta catatgcgct
    17581 ggcagtttcg cgacctcact ttctggcagc tgcgctttcg caccaacact cctatcacca
    17641 gcggtggtag cgatgacagt ctggaagcca gtcacatcag cgatctgttt cttcgtcaat
    17701 ttgaccattt cgatctccgc gacagtgaag tcagtttcct gacgccatcc ggtcagcgtg
    17761 ccgagctggc gatcccacaa ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag
    17821 gcctggtaag cctctccagc cttaccggac agcacggcgt gatgcaggtg cgcatggatt
    17881 tgcgcgatga tgaggggttg ttaagcaatg gtcgcgtctg gctacaggcg gatgacatcg
    17941 acctgaagcc gtggctcggt aaatggatgc aggacaatat tgctctggaa acggcacagt
    18001 tctcccttga aggctggatg acgatcgaca aaggcgatgt aaccggcggt gacgtctggc
    18061 tgaaacaggg cggtgccagc tggttgggcg agaagcaaac gcatacgctg tcggtggata
    18121 atctgaccgc gcatattacg cgtgaaaatc cgggctggca gttctctatt cccgatacac
    18181 ggatcacgat ggacggcaaa ccctggccga gcggagcatt gacgctggcc tggataccgg
    18241 aacaggacgt tggcggcaaa gacaataaac gcagtgacga actccggatt cgcgccagta
    18301 atctggagct ggcaggcctg gagggcatac gcccgctggc cgcgaaactt tcacctgcac
    18361 tgggtgatgt ttggcgctcc acacaaccga gcggcaagat taacactctg gcgctggata
    18421 tcccgcttca ggcggcagac aagacccgtt ttcaggcatc gtggagcgat ctggcctgga
    18481 agcaatggaa attattaccg ggtgcggaac acttctccgg gacgctttcc ggcagcgttg
    18541 aaaatggttt gcttaccgcg tcgatgaagc aggcaaagat gccttacgaa acggtattcc
    18601 gtgcgccact ggaaatcgcc gacggccagg caactataag ctggctgaac aatgacaaag
    18661 gtttccagct ggatgggcgt aatattgacg ttaaagccaa agccgtccat gcgcgcggcg
    18721 gttttcgtta cctgcaacct gctaacgatg aaccctggct gggtattctg gctggcatca
    18781 gtaccgatga tggttcacaa gcctggcact atttcccgga aaacttgatg ggtaaagacc
    18841 tggttgatta cttaagtggc gcgattcagg gcggtgaagc ggataacgcg acgctggttt
    18901 atggtggcaa tccgcaactc ttcccctata aacacaacga aggtcagttt gaagtgctgg
    18961 tgccgctgcg caacgcgaag tttgccttcc agccggactg gcctgcatta actaaccttg
    19021 atattgaact ggactttatt aacgacggtt tatggatgaa aaccgatggc gttaatctgg
    19081 gcggcgtgcg cgcgagtaat ctcaccgcag tgatccctga ctactcgaaa gaaaaactgc
    19141 tgattgacgc tgacattaaa ggtccgggta aagccgttgg cccttacttt gatgagacac
    19201 cgctgaaaga ttctctgggt gcgaccctgc aagaactcca gctcgacggc gatgtgaatg
    19261 ctcgcttaca tcttgatatc ccgctgaacg gcgagctggt aaccgcgaaa ggtgaagtga
    19321 cgctgcgtaa taacagtctg tttatcaaac cactcgacag caccctgaaa aatttgagcg
    19381 gtaaattcag ctttatcaat ggcgatctgc aaagtgaacc actgacagca agctggttta
    19441 atcagccgtt gaacgtggat ttttccacca aagaaggggc aaaagcctac caggttgcgg
    19501 tgaatctcaa cggtaactgg caaccggcga aaaccggcgt tctgcctgaa gcggtgaacg
    19561 aagcattgag tggcagcgtg gcgtgggatg gtaaagtggg cattgatctg ccttatcatg
    19621 ctggcgcgac ctataacgta gagctgaacg gcgatctgaa taatgtgagt agtcacttac
    19681 cttcaccgtt agccaaacct gcgggtgaac cactagcggt aaacgttaag gttgatggca
    19741 atctcaacag ctttgaatta accggacagg ctggtgcgga taaccatttc aatagccgct
    19801 ggttgcttgg tcaaaagctg acgctcgatc gtgctatttg ggcggcagac agtaaaacgc
    19861 tcccgccgtt gccggaacaa agtggcgttg aactcaatat gccgccgatg aatggtgccg
    19921 agtggctggc cctgttccag aaaggcgctg cggagagtgt cggtggtgca gcgagtttcc
    19981 cacaatacat aacgttacgt acgcctatgt tgtcactggg aaatcagcaa tggaataacc
    20041 tgagtattgt ttcgcaaccg acggcaaatg gcaccctggt tgaggcgcaa gggcgtgaaa
    20101 tcaacgccac gctagcgatg cgtaataacg cgccgtggct ggcgaatatc aaatatcttt
    20161 attacaaccc gagcgtggcg aaaactcgtg gtgattcaac gccgtcatca cctttcccga
    20221 caacggagcg cattaacttc cgtggctggc cggacgcaca aatacgatgc acagagtgct
    20281 ggttctgggg gcaaaaattc ggtcgcattg acagtgatat caccatttct ggcgatacgt
    20341 taacgctgac caatggactg attgatactg gtttctcgcg gcttactgcc gatggtgaat
    20401 gggttaataa tccggggaat gaacgtacct cgctgaaagg aaaactgcgc gggcagaaaa
    20461 ttgatgccgc cgcagaattt tttggtgtca cgacgcccat acgccagtcg tcatttaatg
    20521 tggattacga tttacactgg cgcaaagcac cctggcagcc agatgaagcg acgttgaatg
    20581 gcatcattca tactcaactg ggtaaaggcg aaattaccga aatcaatacc ggacatgccg
    20641 ggcaattgct gcgcttattg agcgtagatg ccctgatgcg taagctgcgt tttgatttca
    20701 gagacacttt tggcgaaggg ttctattttg actccattcg cagcaccgcg tggattaaag
    20761 acggggttat gcacaccgac gacacgctgg tggatggcct ggaggcggat atcgccatga
    20821 aagggtcggt aaatctggta cgtcgcgacc tgaatatgga agcggttgtc gcaccagaga
    20881 tttctgcgac ggtgggcgtg gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag
    20941 tttttgccgc tagtaaagtg ctggggccgc tgtggagcaa agtctccatt ttgcgctatc
    21001 acatttcggg tccgctggac gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag
    21061 aaaaagcgca atgatttgac gagggcgcgt aattgcccca atctcatatg ataatcgttg
    21121 ccaaaggcca acgagccaga acataaccgt aggccggata gggcgttcat gccgcatccg
    21181 gcagccgtaa aaaatcctct actgcagtaa ctaacgagta gcaaaaacga tgagtctgaa
    21241 cctggtaagt gaacaattgc tagcggcgaa cagcctgaaa catcaggact tgttcgcgat
    21301 cctcggtcaa ctggccgaac gtcgccttga ttatggcgat ctctattttc agtcgagcta
    21361 tcacgaatcc tgggttttag aagaccgcat tattaaagat ggttcttaca acatcgatca
    21421 gggcgttggc gtgcgtgcaa tcagcggtga aaaaaccgga tttgcttacg ctgaccaaat
    21481 cagcctgctg gcgctggaac agagtgcgca agcggcgcgc accatcgtcc gtgatagtgg
    21541 tgatggcaaa gtacagacgc tgggcgcggt agagcatagc ccgttgtata cctcggtaga
    21601 tccgctgcaa agcatgagcc gtgaagagaa gctggatatc ctgcgtcgcg tcgataaggt
    21661 tgctcgcgaa gcggacaagc gcgtacagga agtgactgcc agcctcagtg gtgtctatga
    21721 attaattctg gttgcggcca ccgacggcac gctagcggcg gatgtccgtc cgctggtgcg
    21781 tctttccgtg agcgttctgg tcgaagaaga tggcaaacgc gaacgcggtg ccagtggcgg
    21841 cggcggtcgt tttggttatg agttcttcct tgccgatctt gacggcgaag tccgtgcgga
    21901 tgcatgggca aaagaagcag tgcgtatggc gctggtcaat ctttctgccg ttgctgcacc
    21961 agcgggcacc atgccggtag tacttggcgc aggttggccg ggcgtgctgt tgcatgaagc
    22021 ggtaggtcac ggtctggaag gcgacttcaa ccgccgcggc acttcagtat ttagtgggca
    22081 ggtcggggag ctggtggctt cagaactgtg taccgtggtt gatgacggca cgatggtcga
    22141 tcgccgtggt tcggtggcga ttgatgacga aggtacgcca ggccagtaca acgtgctgat
    22201 tgagaacggc attctgaaag gctacatgca ggataaactc aacgcgcgtt tgatggggat
    22261 gacgccgact ggcaacggtc gccgtgaatc ctacgcccat ctgcccatgc cgcgtatgac
    22321 taacacctat atgctgccgg gtaaatcgac cccgcaggaa attattgaat ccgttgagta
    22381 cggtatctat gcaccgaact ttggtggcgg tcaggtggat atcacctccg gcaaattcgt
    22441 tttctccact tcagaagcat atctgattga aaacggtaaa gtaacgaagc cggtgaaagg
    22501 cgcaacgttg attggttccg gtatcgaaac catgcagcag atttcgatgg ttggcaacga
    22561 cctgaaactg gataacggcg tgggtgtctg cggtaaagaa gggcaaagtt tgccggttgg
    22621 cgtgggccag ccaacgctga aagttgataa cctgactgtt ggcggtactg cgtaataatt
    22681 tcattatttt cagaaggata attaatcttt ctacgtgcac gaacggtccc ctcgctcctc
    22741 tggggttagg gtgaggggaa cccgttggca caggtttgta gaacgtaaca gtacaatatg
    22801 aattacttct ctttcccgcg cccgtgcatc tcctgaaaca atttaccgac ctcaacaaaa
    22861 taatccgtca gcgagttgat cacgacctgt accttcagcg gcagcttatc tttttcggta
    22921 tataacgcat aaaccgggcg tggatctgac tgataacgcg gcagcaggat ctccagctca
    22981 ccgcgattga tctcgttgat cacccacatc agcggcacgt aggcgatccc ggcacccgcc
    23041 gtcagccagc gcaccagcgt catcggatca ttagtcacaa atcttccttg tgggatcagg
    23101 cgagtcgaga tcccttccgg tgcgatcagt tcaaattcat tgtcgggccg cacgctgtat
    23161 tcaagccatg aatgactact caaatcggcg ggtttttccg gtatgccgta ttgtgtgaga
    23221 tagcttttcg cggcgcacac caccatcggc atcgcgccca gacggcggga aaacaggctg
    23281 gaattccggt aaccagattg acgctcaaac ctgggtattc tttcagcatt tttgctgtca
    23341 gcccggcgag aacattttgt gccatagttg aagaacagcc aatgcgcagc gtcccgatgg
    23401 gggtgttatt gaaggcatac agttgctcat gaacatcctg cacttcatga agcatccgac
    23461 ggcagccctg gtagtaaatt ctgccggctt cggtcaggcc aatgctgcgt gtgctacggt
    23521 ttaacagctt tacctgcaac tcatcttcca gttttgacac cgtctgactg atggacgaaa
    23581 cgctcatctg tagctgtctg gcggcggcgg taaaagagcc aaattcaact actttggcaa
    23641 acaccgacat gcgttttagt cgttccatta ttcactctga cttaaaagtg atttagatca
    23701 cataatatag ataacagcat aacagttacg ctaatatatt aaatatcaat ctacagcaat
    23761 gttgctctcg cccggctttc catgcccttc tctgcggcga cagatgctga aaataataac
    23821 gcctgctctc tctttacaac caaggtcaac atgagtctgt ttcccgttat cgtggtgttt
    23881 ggactgtcct tcccaccgat attttttgaa ttgcttttat cactggcgat tttctggctg
    23941 gtgcgccggg tacttgtgcc aacaggtatc tacgactttg tctggcatcc ggcgttgttc
    24001 aacaccgcgc tctattgctg cttgttttat ttgatatcgc gactgttcgt ttgaggttga
    24061 agtgaaaaca ctaataagaa aattctcccg tacggccatc acggtcgtat tagtcattct
    24121 agccttcatc gcaattttta atgcctgggt ctattacacc gaatccccct ggacgcgtga
    24181 cgcgcgcttt agcgctgacg tcgttgcgat cgcgccggac gtttctggac tcattaccca
    24241 ggtgaatgtt catgataacc agctggtgaa aaaaggacag atactgttca ccatcgacca
    24301 gccgcgctat caaaaggcgc ttgaggaagc gcaagccgat gttgcttatt atcaggtact
    24361 ggcacaggag aaacgccagg aggccggacg tcgtaaccgt ctcggtgtgc aggcgatgtc
    24421 tcgcgaagag atcgaccagg ccaacaacgt actacaaacg gttctgcatc agttagcgaa
    24481 agcgcaggcg acccgcgatc tggcaaaact ggatcttgaa cgcacggtga tccgcgcgcc
    24541 agcagatggc tgggtgacca acctcaacgt ctataccggt gagtttatta ctcgaggatc
    24601 aacggcggtt gcgctggtga aacagaactc cttctatgta ctggcctata tggaagaaac
    24661 taagctggaa ggggggcgtc cggggtatcg tgcagagatc acgccgcttg gcagtaacaa
    24721 agtgctgaaa gggactgttg atagtgttgc cgcaggggtc accaacgcca gcagcacgcg
    24781 tgacgacaaa gggatggcga ctatagactc taatcttgaa tgggtgcgtc ttgcgcaacg
    24841 tgttccggtt cgtattcgtc tcgacaacca gcaagagaac atctggcctg cgggcaccac
    24901 tgctacagtg gtggtcactg gcaaacaaga tcgcgacgaa agccaggatt cgttcttccg
    24961 taaaatggcc catcgcctgc gtgagtttgg ttaatcacga tgggtatttt ctccattgct
    25021 aaccaacata ttcgctttgc ggtaaaactg gcgaccgcca ttgtactggc gctgtttgtt
    25081 ggctttcact tccagctgga aacgccacgc tgggcggtac tgacagcggc gattgttgcc
    25141 gccggtccgg cctttgctgc gggaggtgaa ccgtattctg gcgctattcg ctatcgtggc
    25201 tttttgcgca tcatcggcac atttattggc tgtattgccg gactggtgat catcattgcg
    25261 atgatccgcg caccattatt gatgattctg gtgtgctgta tctgggccgg tttttgtacc
    25321 tggatatcct cgctggtacg aatagaaaac tcgtacgcgt gggggctggc cggttatacc
    25381 gcgctgatca ttgtgatcac cattcagccg gaaccattgc ttacgccgca gtttgccgtc
    25441 gaacgttgta gcgagatcgt tatcggtatt gtgtgtgcga ttatggcgga tttgctcttt
    25501 tctccgcgat cgatcaaaca agaagtagat cgagagctgg aaagtttgct ggtcgcgcaa
    25561 tatcaattaa tgcaactctg tatcaagcat ggcgatggtg aagttgtcga taaagcctgg
    25621 ggcgacctgg tgcgacgcac cacggcacta caaggcatgc gcagcaacct gaatatggaa
    25681 tcttcccgct gggcgcgggc caatcgacgt ttaaaagcga tcaatacgct atcgctgacg
    25741 ctgattaccc aatcctgcga aacttatctt attcagaata cgcgcccgga attgatcact
    25801 gatactttcc gcgaattttt tgacacgccg gtagaaaccg cgcaggacgt ccacaagcag
    25861 ctcaaacgcc tgcggagagt tatcgcctgg accggggaac gggaaacgcc tgtcaccatt
    25921 tatagctggg tcgcggcggc aacgcgttat cagcttctca agcgcggcgt tatcagtaac
    25981 acaaaaatca acgccaccga agaagagatc ctgcaaggcg aaccggaagt aaaagtagag
    26041 tcagccgaac gtcatcatgc aatggttaac ttctggcgaa ccacactttc ctgcattctg
    26101 ggcacgcttt tctggctgtg gacgggctgg acttccggca gtggtgcaat ggtgatgatt
    26161 gcggtcgtga cgtcactggc aatgcgtttg ccgaatccac gcatggtggc gatcgacttt
    26221 atctatggga cgctggccgc gctgccgtta gggctgctct actttttggt gattatccct
    26281 aatacccaac agagcatgtt gctgctgtgt attagcctgg cagtgctggg attcttcctc
    26341 ggtatagaag tacagaaacg gcgactgggc tcgatggggg cactggccag caccataaat
    26401 attatcgtgc tggataaccc gatgactttc catttcagtc agtttctcga cagcgcatta
    26461 gggcaaatcg tcggctgtgt gctcgcgttc accgttattt tgctggtgcg ggataaatcg
    26521 cgcgacagga ctggacgtgt actgcttaat cagtttgttt ttgccgctgt ttccgcgatg
    26581 actaccaatg tggcacgtcg taaagagaac cacctcccgg cactttatca gcagctgttt
    26641 ttgctgatga ataagttccc aggggatttg ccgaaatttc gcctggcgct gacgatgatt
    26701 atcgcgcacc agcgcctgcg tgatgcgccg atcccggtta acgaggattt atcggcgttt
    26761 caccgacaaa tgcgccgcac agcagaccat gtgatatctg cccgtagcga tgataaacgt
    26821 cgtcggtact ttggccagtt gctggaagaa ctggaaatct accaggaaaa gctacgcatc
    26881 tggcaagcgc caccgcaggt gacggaaccg gttcatcggc tggcggggat gctccataag
    26941 tatcaacatg cgttgaccga tagttaagtc aaaaccgacg ccaaaagcgt cggttttttc
    27001 atggctatac ttagcgatgc acggcagaac tcgccgcgaa acgtgacggt ggcaacagat
    27061 gaatatttat acctttgatt ttgatgagat tgagagtcag gaggattttt atcgtgactt
    27121 tagccaaacc tttggtctgg ctaaagataa ggtacgcgat ctcgactcac tatgggatgt
    27181 gttaatgaac gatgtcctgc cgctaccact tgagattgaa tttgttcatc tgggagagaa
    27241 aacgcgtcgc cgttttggcg cgttaatatt gctgtttgat gaggcagagg aagagctgga
    27301 agggcatttg cgttttaatg ttcgtcatta gcgcaaaaaa aagcccccga accgggggca
    27361 atatcgtcgg acaagacgat gagggtttat ttgtacagtt cagccgtagc gtgccaggtg
    27421 tcaccgctac gagcttcagt aatctggtag gccgttgcgc ctttctcttc cgcttttttg
    27481 ttcagcattt cacgcatatc cattggcgaa gacgccacac cacttacgga tacggtcccg
    27541 attgcttcac gattttgtgc ttgtgcagta tcaatggagt cggcagcgaa tgcaccgaaa
    27601 gagagaacag aaagtacgct taatgcagct acagtggttt tgattttcat gatttttacc
    27661 tcgacataat cttttagctg ggcctttgtt tcgtgatcca tatcacaaaa tcaagcatac
    27721 actaattatt gaactaatta ataccacact aactgtttca gttgatacta agtgttaaaa
    27781 atgtgaattt taataacaaa aaagaattaa aaacacggtt atttattgtt aattctagta
    27841 ttttcaatta gataatgaat tttacataat gtgctttttt taccgtatgt attcattgtg
    27901 tgaatgatat gtcgcggtaa aacgcactat tcgttaaatt aatcgcaggg gaaagcaggg
    27961 ggttgagagg aataagcaac attttccccg ccgccagaag cgacggggca gagattaaag
    28021 ctcctggtcg aacagctcta aaatcgcttc gtacaggtct ttgacggtga aaccgttagc
    28081 aggggtggta aagatggtgt catcgccagc gatggtgccc agaatacctt ctgctttgcc
    28141 cagtgagtcc agcaggcgag caattaactg cgccgcgcca gggctggtat gaatcacgac
    28201 aactgcatcg ttgtagtcga tatccagcac cagattcttc aatggactgg aggtggttgg
    28261 tacacccagt tcagctggca ggcagtaaac catttccatt ttggcattgc gtgtacgtac
    28321 agcaccaaac ttggtcaaca tccgcgagac tttagactga ttaatattgt caaagccttg
    28381 ctcctgcaac gcggcgacga tttcgccctg ggagctaaat ttctcttctt taagtaatgc
    28441 tttaaatgct ttaactagtt cttcttgctt agccgagctt cgcataagtc acccgatatg
    28501 gtggttgata caacattatt gtgcatacag atgaattttt atgcaaacag tcagccctga
    28561 agaaggctga aataatgtta tgaaagagcg ggattttatc aaatttcgtt attgagaaac
    28621 atgcctgcgt cacggcatgc aaattctgtt taaaagtaaa ttaattgtta tcaaattgat
    28681 gttgttttgg ctgaacggta gggtatattg tcaccacctg ttggaatgtt gcgctaatgc
    28741 ataagcgact gttaattacg taagttaggt tcctgattac ggcaattaaa tgcataaacg
    28801 ctaaacttgc gtgactacac attcttgaga tgtggtcatt gtaaacggca attttgtgga
    28861 ttaaggtcgc ggcagcggag caacatatct tagtttatca atataataag gagtttagga
    28921 tgaaagtcgc agtcctcggc gctgctggcg gtattggcca ggcgcttgca ctactgttaa
    28981 aaacccaact gccttcaggt tcagaactct ctctgtatga tatcgctcca gtgactcccg
    29041 gtgtggctgt cgatctgagc catatcccta ctgctgtgaa aatcaaaggt ttttctggtg
    29101 aagatgcgac tccggcgctg gaaggcgcag atgtcgttct tatctctgca ggtgtagcgc
    29161 gtaaaccggg tatggatcgt tccgacctgt ttaacgttaa cgccggcatc gtgaaaaacc
    29221 tggtacagca agttgcgaaa acctgcccga aagcgtgcat tggtattatc actaacccgg
    29281 ttaacaccac agttgcgatt gctgctgaag tgctgaaaaa agccggtgtt tatgacaaaa
    29341 acaaactgtt cggcgttacc acgctggaca tcattcgttc caacactttt gttgcggaac
    29401 tgaaaggcaa acagccaggc gaagttgaag tgccggttat tggcggtcac tctggtgtta
    29461 ccattctgcc gctgctgtca caggttcctg gcgttagttt taccgagcag gaagtggctg
    29521 atctgaccaa acgcatccag aacgcgggta ctgaagtggt tgaagcgaaa gccggtggcg
    29581 ggtctgcaac cctgtctatg ggccaggcag ctgcacgttt tggtctgtct ctggttcgtg
    29641 cactgcaggg cgaacaaggc gttgtcgaat gtgcctacgt tgaaggcgat ggtcagtacg
    29701 cccgtttctt ctctcaaccg ctgctgctgg gtaaaaacgg cgtggaagag cgtaaatcta
    29761 tcggtaccct gagcgcattt gaacagaacg cgctggaagg tatgctggat acactcaaga
    29821 aagatatcgc cttgggcgaa gagttcgtta ataagtaatt gattagcgaa taataaaaaa
    29881 ccggagcaca gac
//
LOCUS       DAZOXR010000034        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZOXR010000034 DAZOXR010000000
VERSION     DAZOXR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.034"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHUNN_001294"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHUNN_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR0575718.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHUNN_001295"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHUNN_001295"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0575719.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUNN_001296"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUNN_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR0575720.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHUNN_001297"
     CDS             complement(3644..4030)
                     /locus_tag="ACHUNN_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0575721.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUNN_001298"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUNN_001298"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR0575722.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUNN_001299"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUNN_001299"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0575723.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUNN_001300"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUNN_001300"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0575724.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUNN_001301"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUNN_001301"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0575725.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUNN_001302"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUNN_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0575726.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUNN_001303"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUNN_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0575727.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUNN_001304"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUNN_001304"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0575728.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHUNN_001305"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHUNN_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0575729.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUNN_001306"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUNN_001306"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0575730.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUNN_001307"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUNN_001307"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0575731.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUNN_001308"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUNN_001308"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0575732.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUNN_001309"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUNN_001309"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0575733.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUNN_001310"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUNN_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0575734.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHUNN_001311"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHUNN_001311"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0575735.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUNN_001312"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUNN_001312"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0575736.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUNN_001313"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUNN_001313"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0575737.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUNN_001314"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUNN_001314"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0575738.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUNN_001315"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUNN_001315"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0575739.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUNN_001316"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUNN_001316"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0575740.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUNN_001317"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUNN_001317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0575741.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHUNN_001318"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHUNN_001318"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0575742.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZOXR010000035        29372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZOXR010000035 DAZOXR010000000
VERSION     DAZOXR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.035"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..305
                     /locus_tag="ACHUNN_001319"
     CDS             <1..305
                     /locus_tag="ACHUNN_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0575743.1"
                     /translation="HKQHMDDRLIHLIRSNAVASTPVYENYFNNTTAFRPKPEDIFKC
                     GQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            567..2189
                     /locus_tag="ACHUNN_001320"
     CDS             567..2189
                     /locus_tag="ACHUNN_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0575744.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2379..2492
                     /gene="yhiY"
                     /locus_tag="ACHUNN_001321"
     CDS             2379..2492
                     /gene="yhiY"
                     /locus_tag="ACHUNN_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0575745.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2555..3622
                     /locus_tag="ACHUNN_001322"
     CDS             2555..3622
                     /locus_tag="ACHUNN_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0575746.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            3619..6354
                     /gene="rbbA"
                     /locus_tag="ACHUNN_001323"
     CDS             3619..6354
                     /gene="rbbA"
                     /locus_tag="ACHUNN_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0575747.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6354..7478
                     /locus_tag="ACHUNN_001324"
     CDS             6354..7478
                     /locus_tag="ACHUNN_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0575748.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7551..7824
                     /locus_tag="ACHUNN_001325"
                     /pseudo
     CDS             7551..7824
                     /locus_tag="ACHUNN_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            7821..8180
                     /locus_tag="ACHUNN_001326"
     CDS             7821..8180
                     /locus_tag="ACHUNN_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0575749.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8300..8701)
                     /gene="nikR"
                     /locus_tag="ACHUNN_001327"
     CDS             complement(8300..8701)
                     /gene="nikR"
                     /locus_tag="ACHUNN_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0575750.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(8707..9513)
                     /gene="nikE"
                     /locus_tag="ACHUNN_001328"
     CDS             complement(8707..9513)
                     /gene="nikE"
                     /locus_tag="ACHUNN_001328"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0575751.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9510..10274)
                     /gene="nikD"
                     /locus_tag="ACHUNN_001329"
     CDS             complement(9510..10274)
                     /gene="nikD"
                     /locus_tag="ACHUNN_001329"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0575752.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(10274..11107)
                     /gene="nikC"
                     /locus_tag="ACHUNN_001330"
     CDS             complement(10274..11107)
                     /gene="nikC"
                     /locus_tag="ACHUNN_001330"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0575753.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11104..12048)
                     /gene="nikB"
                     /locus_tag="ACHUNN_001331"
     CDS             complement(11104..12048)
                     /gene="nikB"
                     /locus_tag="ACHUNN_001331"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0575754.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12048..13622)
                     /gene="nikA"
                     /locus_tag="ACHUNN_001332"
     CDS             complement(12048..13622)
                     /gene="nikA"
                     /locus_tag="ACHUNN_001332"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0575755.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(13733..14320)
                     /gene="acpT"
                     /locus_tag="ACHUNN_001333"
     CDS             complement(13733..14320)
                     /gene="acpT"
                     /locus_tag="ACHUNN_001333"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0575756.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14375..15424)
                     /locus_tag="ACHUNN_001334"
     CDS             complement(14375..15424)
                     /locus_tag="ACHUNN_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0575757.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15556..16773
                     /locus_tag="ACHUNN_001335"
     CDS             15556..16773
                     /locus_tag="ACHUNN_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575758.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16777..17334)
                     /gene="dcrB"
                     /locus_tag="ACHUNN_001336"
     CDS             complement(16777..17334)
                     /gene="dcrB"
                     /locus_tag="ACHUNN_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0575759.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17407..18072)
                     /locus_tag="ACHUNN_001337"
     CDS             complement(17407..18072)
                     /locus_tag="ACHUNN_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0575760.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            18293..18538
                     /gene="tusA"
                     /locus_tag="ACHUNN_001338"
     CDS             18293..18538
                     /gene="tusA"
                     /locus_tag="ACHUNN_001338"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0575761.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18640..20838)
                     /gene="zntA"
                     /locus_tag="ACHUNN_001339"
     CDS             complement(18640..20838)
                     /gene="zntA"
                     /locus_tag="ACHUNN_001339"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0575762.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(20912..21538)
                     /locus_tag="ACHUNN_001340"
     CDS             complement(20912..21538)
                     /locus_tag="ACHUNN_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0575763.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            21679..22038
                     /locus_tag="ACHUNN_001341"
     CDS             21679..22038
                     /locus_tag="ACHUNN_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0575764.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22041..22310)
                     /locus_tag="ACHUNN_001342"
     CDS             complement(22041..22310)
                     /locus_tag="ACHUNN_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0575765.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(22300..22896)
                     /gene="rsmD"
                     /locus_tag="ACHUNN_001343"
     CDS             complement(22300..22896)
                     /gene="rsmD"
                     /locus_tag="ACHUNN_001343"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0575766.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23046..24542
                     /gene="ftsY"
                     /locus_tag="ACHUNN_001344"
     CDS             23046..24542
                     /gene="ftsY"
                     /locus_tag="ACHUNN_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0575767.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24545..25213
                     /gene="ftsE"
                     /locus_tag="ACHUNN_001345"
     CDS             24545..25213
                     /gene="ftsE"
                     /locus_tag="ACHUNN_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0575768.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            25206..26264
                     /gene="ftsX"
                     /locus_tag="ACHUNN_001346"
     CDS             25206..26264
                     /gene="ftsX"
                     /locus_tag="ACHUNN_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0575769.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26509..27363
                     /gene="rpoH"
                     /locus_tag="ACHUNN_001347"
     CDS             26509..27363
                     /gene="rpoH"
                     /locus_tag="ACHUNN_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0575770.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27634..28737
                     /gene="livJ"
                     /locus_tag="ACHUNN_001348"
     CDS             27634..28737
                     /gene="livJ"
                     /locus_tag="ACHUNN_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0575771.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(28936..29319)
                     /gene="panM"
                     /locus_tag="ACHUNN_001349"
     CDS             complement(28936..29319)
                     /gene="panM"
                     /locus_tag="ACHUNN_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0575772.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         7117 a         7508 c         8025 g         6722 t
ORIGIN      
        1 aacataaaca acacatggat gatcgtctta tccatctaat tagaagcaat gctgtagcat
       61 ccacacctgt atatgaaaat tattttaata acactactgc tttcagacca aaacctgaag
      121 atatcttcaa atgtggacag acgctcaaaa aaactaaaca gaaatatggc cattatggat
      181 taggggtaga tcagcatcaa aaaattgata attataccat ggggctaaat tcctggaaga
      241 tagcacctaa cgaacgtggt gataaaaaag gtgtcccagg gcttagttgt tttcaacctc
      301 aataaaactt catgagtgct gtggtatctc tttttaattt tgccagtatt tcataggttt
      361 tcttaatcga ttgtagaggc atgttctgat gacgcctcta caatctttag ggactttctc
      421 tgctatgaga taagaaattt catattatgc tttaaggctg aatgatttgt gcaaaatata
      481 attattttcc catcgaacat gaggtgtatg tcataagatt aaatgttaac tatgtattcc
      541 tcgcggtaaa taatatgagg taaaagatga aaattggaac tgtggcaggc actaacgaca
      601 gtacaacgac aatcacaaca aatgatatgg tgcaagagca tgttaccaac ttcactaaag
      661 aactgtttgg gtatattgca aatggtatag gtgatgatat atcaagtatt gccaggacaa
      721 tgctcggtga ggttgtggaa aaaatagatg attggcaaat agaacggttc caacaatcta
      781 ttcaagatga caaaatttca tttaccatac aaacgaatca ttcagaaaaa tatagtatgc
      841 tatcgggcat gcgcgctcat attctacgta ggaataactg ttatcagttt attgtgacaa
      901 ttaatagtaa aaattacggt tgccctcttg ataataccga tattaattgg tgcagcattg
      961 tttatttact taataatatg acagtaaatg ataatgctaa tgatgttgca gttactgagt
     1021 cgtacaaacc catctggaac tggaaaatat cgcaatacaa tgtatttgat attaaattcg
     1081 aaacaatgat aaaaccccag ttcgctgaca gaacctattt ctccaattgt tcacctgttg
     1141 atcccacaag taccagaccg acctattttg gcgataccga tggctcagtc ggtgcagttt
     1201 tgtacgcgct ttttgcaacg ggacatttgg gaatcatggc tgaaggagaa aatttcctta
     1261 gccagttgct taatatagaa gatgaagttc tcaacgtatt gctaagggag aactttaatg
     1321 agcaacttga cactaatgtt aatataataa tcagtatcct gaacagaaga gacattgttt
     1381 tagaaagtct tcagccttat ttggttatta ataaagacgc agtcacgcca tgtacgtttc
     1441 tgggcgacca gactggcgat cgatttagta atatctgtgg cgataaattt atcatcgatt
     1501 tattaaaacg cataatgagt atcaatgaaa acgttcatgt attagctgga aatcatgaaa
     1561 cgaattgcaa tggtaactat atgcagaact tcacgcgcat gaaaccactt gatgaggata
     1621 cctactctgg gataaaagat tatcctgtat gtttctatga ccccaaatac aaaataatgg
     1681 caaatcatca tgggataact tttgatgatc agcgaaaacg ttacattata ggaccgataa
     1741 cagtctctag tgatgaaatg accaatgctc tcgatccggt cgaattagcg gcaattatca
     1801 ataaaaagca ccatgctgta attaatggca aaaagtttaa aaatagccgg gctatctctt
     1861 gtcgatcctt taatcgttat ttttctgttt cgacagatta cagacctaaa cttgaagcac
     1921 tattagcatg ctcacaaatg ttaggtataa atcaagttgt tgcacataac ggtaatggtg
     1981 gaagagaatg tataggtgaa acggggacag ttttaggtct taacgcccgt gacagtaaac
     2041 atgctggaag aatgttcagc atgcataatt gccaaattaa ccctggtgca gggcctgaga
     2101 taaccactcc ctggaagtct taccagcatg aaaaaaacag aaatggactt atgcctttga
     2161 tcagaaggcg cacaatgtta cagctctgaa ccatagacaa catgaatatt aatgttacta
     2221 tctggttgag actgttgata aaacgtaaaa aggaatgctc tgttcctgca actgaacagg
     2281 cgtgaaacgc tatccaacag gatggatacc gttttgtcag ccagttttat agatcacact
     2341 tatgagtatg cgtgtttcat ttacggccag gaatgctgat gatgacaaca ttactacccg
     2401 ttttcactaa gccttccccg ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt
     2461 tccttcttat cccggatgga agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc
     2521 gtcgtttgct tgcagggatt atggcgggtc gattatggat aagagtaagc gccatctggc
     2581 gtggtgggtt gtcgggttac tggtggtggc tgccgtcgtg gcgtggtggc tgttgcgccc
     2641 ggcgggtgtg ccggaaggct ttgctgtcag caatgggcgc attgaagcga cggaagtgga
     2701 tatcgccagc aaaattgccg ggcgtatcga caccattctg gtgaaagaag gccagtttgt
     2761 tcgcgaaggc gaagtgctgg cgaagatgga tactcgcgtg ttgcaggagc agcgactgga
     2821 agccatcgcg caaatcaaag aagcacaaag tgccgttgct gccgcgcagg ctttgctgga
     2881 gcaacggcaa agcgaaactc gtgccgcgca gtcgctggtt aatcaacgcc agtccgaact
     2941 ggactccgtg gctaaacgtc atacgcgttc ccgctcgctc gcccaacgag gggctatttc
     3001 tgcgcaacag ctggatgacg atcgcgccgc cgctgagagc gcccgagctg cgctggaatc
     3061 ggcaaaagct caggtatcgg cttctaaagc ggctatagaa gcggcccgta ccaatatcat
     3121 tcaggcccaa actcgcgtcg aagccgcgca agccactgaa cggcgtattg ccgcagatat
     3181 cgatgacagt gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg ttgctgagcc
     3241 aggggaagtg ctggcggcag gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta
     3301 tatgactttc ttcctgccaa ccgaacaggc gggcacgctg aaacttggcg gtgaagcccg
     3361 gctgatcctc gatgccgcgc cagatctgcg tattccggcg accatcagtt ttgtcgccag
     3421 tgtcgcccag ttcacgccaa aaaccgtcga aaccagcgat gaacggctga aactgatgtt
     3481 ccgcgtcaaa gcgcgtatcc caccggaatt actccagcag catctggaat atgtcaaaac
     3541 cggtttaccg ggcgtagcgt gggtgcgggt gaatgaagaa ctttcgtggc ctgacgacct
     3601 cgtggtgagg ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa
     3661 ctggcgggcg tgagccagca ttatggaaaa aacgttgcgc tgaacaatat tactctcgat
     3721 attccggccc gctgtatggt cgggctgatt ggtccggatg gcgtcgggaa gtcgagctta
     3781 ttgtcgttga tttccggtgc ccgcgtcatt gagcagggca atgtgatggt gctgggcggc
     3841 gatatgcgcg acccgaggca tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag
     3901 gggctgggca aaaacctcta ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct
     3961 cgcctgttcg gtcacgacaa agcggagcgg gaagtgcgaa ttaatgagct gctgaccagc
     4021 acggggttag caccgtttcg cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa
     4081 aaacttgggc tgtgctgcgc gttaatccac gacccggaac tgttgatcct tgatgagcca
     4141 acaacggggg ttgacccgct ctcccgctcc cagttctggg atctgatcga cagtattcgc
     4201 cagcggcaga gcaatatgag cgtgctggtc gccaccgcct atatggaaga ggccgaacgc
     4261 ttcgactggc tggtagcgat gaatgccgga gaagtgctgg caactggcag cgccgaagag
     4321 ctacggcagc aaacgcaaag cgctacgctg gaagaagcat ttataaatct gttaccgcaa
     4381 gcgcaacgcc aggcgcatca ggcggtagtg atcccaccgt atcaacctga aaacgcagag
     4441 attgccatcg aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc cgttgatcac
     4501 gttaatttcc gcattccacg cggggagatt tttggttttc ttggttcgaa cggctgcggt
     4561 aaatccacca ccatgaaaat gctcaccgga ctgctgcccg ccagcgaagg tgaggcgtgg
     4621 ctgttcgggc aaccggttga tccaaaagat atcgataccc gccgtcgggt gggctatatg
     4681 tcgcaggcgt tttcgctcta taacgaactc accgtgcggc aaaaccttga gttacatgcc
     4741 cgtttgtttc acatcccgga agcggaaatt cccgcaagag tggctgaaat gagcgagcgt
     4801 tttaagctca acgacgttga agatgttctg ccggagtcat tgccgctcgg cattcgccag
     4861 cggctttcgc tggcggtggc ggtgattcat cgcccggaga tgttaatcct cgatgagcct
     4921 acttctggtg tcgatccggt ggcgagggat atgttctggc agttgatggt cgatctctcg
     4981 cgccaggaca aagtgactat cttcatctct acccacttta tgaacgaagc ggaacgttgc
     5041 gaccgcatct cactgatgca cgccggaaaa gtgcttgcca gcggtacacc gcaggaactg
     5101 gttgagaaac gcggagccgc cagtctggaa gaggcattta tcgcctattt gcaggaagcg
     5161 gcagggcaga gcaacgaagc cgaagcgccg cccgtggtac acgacaccac ccacgcgccg
     5221 cgtcagggat ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc gctggaactg
     5281 cgacgcgatc cagtacgttc gacgctggcg ctgatgggaa cggtgatcct gatgctgata
     5341 atgggttacg gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct cgaccgcgac
     5401 cagaccgtca gtagccaggc gtggacactc aacctctccg gttcccgtta ctttatcgaa
     5461 cagccgccgc tcactagtta tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg
     5521 gtggcgattg agatcccgcc caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa
     5581 ctcggcgtct ggatcgacgg agcgatgccc agtcgtgccg aaacggtaaa aggttacgtg
     5641 caggcaatgc accagagttg gcttcaggat gtggcgagcc gacaatcgac acccgccagc
     5701 caaagcgggc tgatgaacat tgagacgcgc tatcgctata acccggacgt aaaaagcctg
     5761 ccagcgattg ttccggcggt gatcccgctt ctgctgatga tgatcccgtc aatgctaagc
     5821 gcccttagcg tggtgcggga aaaagagcta gggtcgatta tcaaccttta cgtgaccccc
     5881 accacccgta gcgaattttt gcttggcaaa cagctgccgt acatcgcgct ggggatgctg
     5941 aactttttcc tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt
     6001 ttcctgacgc tcaccctggc ggcgctgctg tatatcatca ttgccaccgg aatggggctg
     6061 ctgatctcca cctttatgaa aagccagatt gccgccattt tcggaacggc gattatcacg
     6121 ttgatcccgg cgacacagtt ttccgggatg atcgatccgg tagcttcgct ggaagggcct
     6181 ggacgttgga tcggcgaggt ttacccgacc agtcattttc tgactatcgc ccgcggaacg
     6241 ttctcgaaag cgctggatct gactgatttg tggcaacttt ttatcccgtt gctgatagcc
     6301 atcccgctgg tgatgggctt aagcatcctg ctgctgaaaa aacaggaggg atgatgcgcc
     6361 atttacgcaa tatttttaat ctgggtatca aagagttgcg cagtctgctc ggtgataaag
     6421 cgatgctgac gctgattgtc ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta
     6481 cgccaggatc gttgaacctc gcgccgatcg ccattgccga tatggatcaa tcgcagttat
     6541 cgaaccggat cgttaacagc ttctatcgtc cgtggttttt gccaccggag atgatcaccg
     6601 ccgatgagat ggatgccgga ctggacgccg gacgctatac cttcgcgata aatattccgc
     6661 ctaattttca gcgtgatgtc ctcgccggac gccagccgga tattcaggtg aacgtcgatg
     6721 ccacgcgcat gagccaggca tttaccggca atgggtatat ccagaatatt atcaacggtg
     6781 aagtgaacag ctttgtcgcg cgctaccgtg ataacagcga accgttggta tcgctggaaa
     6841 cccggatgcg ctttaacccg aacctcgatc ccgcgtggtt tggcggggtg atggcgatca
     6901 tcaacaacat taccatgctg gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg
     6961 aacacggcac ggtggaacac ttactggtga tgccgataac gccgtttgag atcatgatgg
     7021 cgaagatctg gtcgatgggg ctggtggtgc tggtggtatc gggattatcg ctggtgctga
     7081 tggcgaaagg tgtactgggc gtaccgattg aaggctcgat cccgctgttt atgctgggcg
     7141 tggcgctcag tctgtttgcc accacgtcaa tcggcatttt tatggggacg atagcgcgtt
     7201 caatgccgca actggggctg ctggtgattc tggtgctgct gccgctgcaa atgctttccg
     7261 gtggatccac gccgcgcgaa agtatgccgc agatggtgca ggacattatg ctgaccatgc
     7321 cgacgacaca ctttgttagc ctcgcgcagg ccattctcta ccggggtgcc ggattcgaaa
     7381 tcgtctggcc gcagttcctg acgctgatgg caattggcgg cgcgtttttc accattgcgc
     7441 tgctgcgatt caggaagacg attgggacaa tggcgtaagg aagggaaact tgacttcata
     7501 ctgtgatgtc atactattga tgtcataaag tgaagtcata aggacatgcc atgagaacca
     7561 aggtacaggc tttacggaag aaaaaaaaat acattggatc aaatatttaa aactcctgtt
     7621 cctcaaggga tcaagtggtc tgatatagag tcactggtta aagcattagg cggagaaatt
     7681 aaggaaggaa gaggttcgcg ttgtaagttc atactaaata tgagcgttgc gtgtttccat
     7741 cggcctcatc cgtcgccaga taccgataaa ggcgctgtag aaagcgtgcg tgactggtta
     7801 ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc aaactctatg gagattgccg
     7861 ggcaaccagc tgttattacc tacgtgccag aactgaatgc atttcgtggc aaattcctgg
     7921 gcttgtcagg ttattgtgat tttgtctctg acagcattca ggggctgcaa aaagaagggg
     7981 aactttccct gcgagagtat cttgaggatt gtaaagctgc gggtattgag ccgtatgcgc
     8041 gtacagaaaa gattaagacc tttacgttac gctatccaga atcgctgagt gaacgcctta
     8101 ataatgcggc tgcgcagcaa caagtctctg ttaataccta tattattgaa acactgaatg
     8161 agcgtttgaa tcatttataa attttagcgg cgtacattac gcagaatatc tgcctttaca
     8221 ggatctatct atgcaactga acttaatttt ataaattaag ttcagttaat atcttttctt
     8281 tcctcgtttc agaaataact caatcttcct tcggcaagca ctgcaaatgc ccatgccgca
     8341 cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac gtcgcccata tcacctttca
     8401 gcacggcgat ttccagacag tcgtcgtgat tgatatgcac atgcagcgtg gcgacggaga
     8461 gatcgtggtg atggtgctgg gtggagacaa tgcggctggc taagtcgcgt ttttcgtgtt
     8521 catacacata ggagagcacc gcgaaacctt gcgtgccgtg ctgctgagtg gcctcctggg
     8581 ccagggcgct acgcagaata tcgcggatag cttcggaacg gttgttataa ccacgacgct
     8641 ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag cgtgatggtg actcgttgca
     8701 tttgcgttaa accttttctg tggtgcgacg gcgcacgggg aatgcgggta ataccgcgtt
     8761 ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta tctcccacca cctgggtttc
     8821 gacgatttgt ccgttgtcca tcaccatcac ccgctgacag aagcgttcca ccaggcgtaa
     8881 gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt tgtagctttt tcagcaggcg
     8941 aatgacgccc gcctgtaaca cgagatcaag gttagaaacg gcttcatcca gaatcagtag
     9001 tttcggttcg accgccagcg cgcgagccag gcagacgcgc tggagctggc cgccgcttaa
     9061 ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg accgccttca gcatttcgct
     9121 ggcgcgcgcc agttgttcgg attttttcag tgatagcagg tggcgcatcg gttcacgcag
     9181 gatctcgcgc acggttttgc gcggattcac ggcgctgatg gagtcctgaa ataccatctg
     9241 aatatcgcgg cggaacgctt tacgctgggc gcgattgagt ttcgccagcg gttcgccacg
     9301 ccagctaata ttcccctgcg cgggcgattc taaacccacc agcaaccgcg cgagggtact
     9361 tttcccgcag ccgctgcgcc ccagcagggc gacggtttcg ccgcttttca gggtcaggga
     9421 aacgttattc agcaccgcct gatgttgatg ttttccgtta aatccaccgt gggcatagtg
     9481 atgggaaagg ccggagacgt taagtaaagt catgatgcca gctccatacc gtagagggcg
     9541 agatgagcgg aaaccaggct gcgcgttacc gcatgtttgg gggcgttaaa cagcgtttct
     9601 acatcgccct gttcgacaat tttaccttgt gacatcaccg ccacgtcatc cgccagacgc
     9661 gccacaacgc ccatatcatg ggtgaccagc agcattcccg gcgcttgttt ttgcataatg
     9721 ctttccagca gatcgaggat gcgcgcctgt gctaccacgt cgaggtcggt ggtcggttca
     9781 tcggcgatga taaacggtga ttcacacagc accgccatcg caatcatcat gcgctgcaac
     9841 atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc gcgcggcgtt ttccagcccc
     9901 accgcttcta tggcagcggt aagcgtagcg tcatcggcgg gtttccctaa cgccaggcag
     9961 gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa aggcgctgcg cgggttctgc
    10021 atgatggtgg caattttgat gccgcgcagg gcgcagggat aaaccgcttt gccatcggct
    10081 aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa tgcccagcgt cgcggcgcag
    10141 gttaatgatt tcccgctgcc gctaccgccg actaacgcca gcacgcgccc gcgttgcagg
    10201 gttaacgata caccgtgtac cagcggctgc gcggcctgta gcgcgatatt acgtagttca
    10261 atctgttgcg gcattagtgt gcgtgctccg tcaccagatg aggatccaga tgatcgcgca
    10321 gtgcgtcacc caccaggtta aaggccatca cgctgataaa cagcgccagc cccggccaga
    10381 acatttgcag cggctgggtc cagatatact ggcgcgcgtc gttaatcatc acgccccatt
    10441 cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat ccccgcgacg tgcagcatca
    10501 tatggccgat atccagcgtt gccagcacca gcagcgaagg gatcaccgcg cctgccagat
    10561 gatcgacaaa cacccgcaca tggcccgcac cggaaagccg tgacgccagc acaaactcgc
    10621 gttggcgtag tgaaatcacc aagctgcgca ccatgcgtgc ataccacgcc cagtgcgaca
    10681 gggcgatggc gataattacg ttggtcagcc cggtgccgag cacgccaacc ataaagaacg
    10741 acagaatcga ggtcgggaag gtcataaaca tatcggcgac gcgcatggtg gcctgatcaa
    10801 cgcgcccgcc aatcaacccg gcgctgccgc caataaccag ccctaatgtc agcaccagca
    10861 gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc tgccatcagc cgcgagaaaa
    10921 tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc atccggcgaa agcaggcgcg
    10981 acggcaaatc aatcgcctgg gggtcatacg gcagccacca ctggctggtg agcgcaatca
    11041 gcgccagcag ggcgataatg atcagcgcca ggcgtaccga ccagcgggaa gagaggaaaa
    11101 agttcacgcg tgcgctcctt catgacgacg aatgcgcggg tccagcgcgg cgttgagcaa
    11161 atcgacaatc aaattacaga ccacaaaaac caccaccatc atcagcgtaa agcactgaat
    11221 caccggatag tcacggttaa aaatcgccga caccgcatag cgcccgacgc ccggccaggc
    11281 aaagatgttt tcgataatca tcgtcccgcc aatcagttcg ccgatgtgca tccccacggc
    11341 ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg cgttcggtct gtttgtcgtt
    11401 caggccgcgc agacgcgccc aagtgacgtg acgctgaccg gcgacgtcca gcatactggc
    11461 gcgcagcagt cgcgcgttaa tcgccagcga cataaaggca atggaaaccg caggcaaaat
    11521 gatgtgctgc cagccgccgt aacccatcgc gggtagccat tgcagataca ccgaaaacgc
    11581 catcaccagt aaaaacgcca gccagaagtt aggcatcgac acgccaagaa acgcgatgaa
    11641 acgtacggcg aaatccggca gacggtcgcg atggcgcgcc gcccagatac cgagcggtac
    11701 ggaagtgagc agaattaata ccagcgccgc acctgcaagt tccagcgtgg cgggcaggaa
    11761 gttcagcata tcgtccagca ccgggcgttg gctggcgaat gagataccaa agtcaagatg
    11821 cagcgccttc cacaaccagg taccgtactg gacgtacagc ggctgatcca gcccgagcat
    11881 ggtgcgggta gaggccagca tctccggcgt cggcggcagg ttagataaac gcagataatc
    11941 gagcgccggg tcgccggtac cgaggcgcag catcagaaaa atgatcaccg aggcggcgag
    12001 caccatcggg atcagcagca gaaagcggcg taatacgtaa cgcaacatta aggtttcacc
    12061 ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg cgtaggggat attacccagc
    12121 tccggttttg ataccaccat catcgagatg tagctgatag gcagataaac cgcctcgtca
    12181 tgcagacggg tcaaaatgtc gcgatacagc gcctgacgtt gcgtttcgtc atgggtcgcc
    12241 agcacttcgc cgatctcttt atcaatcagc ggcttgtcgg ctaatccttg ctgtgcctgg
    12301 aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg cgtgtggatc gtatggcgcg
    12361 ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc gctgacgagc atagatactg
    12421 ctctcttctt cgccaatcag cgagacatct gcgccaatct ggcgcatatc agcctgaatg
    12481 atttccgcca tcgatttgct taacgcatcg gtgccgatga acgacagttc aatgcgcagc
    12541 ggctgaccat ttttctcgcg gatgtctttg cccgcaggca gcgtccaacc ggctttttcc
    12601 agcaactctt tcgctttctg cggatcgtac tggcgcggtt tcaggccgag attagcgtaa
    12661 ggcacagaag gggcaaacag ggtgtcggcg acctgcgggg tgccatataa cgcgttatca
    12721 atcagcgatt ttttgtttac cgcgtaatta agagcttcac gtactgccag ctcgttggtg
    12781 ggggctttgg cggtattgag cgccagcatc acggtttcga tcggctgtga cagttgggtg
    12841 tggtaagccg gattctggct aaagcgggcg aaggtatcga gcggtaataa cccttcgttt
    12901 ccgtacagca ggtcgatatc gccagtttca aacgccaccg cgcgggtagt cgggtccggg
    12961 atgacgttaa aggtgatctt tttaatcgct ggcttttcgc cccagtagtt ttcgttacgg
    13021 acgaagacat cgtactgatt cagtttcgat tcctgcaaaa tccacggtcc ggtgccaatc
    13081 ggcgctttaa ttccgttcat ggtttcatgg tttttaaact gcgagggagc gataaaacgg
    13141 aaaggacggg gcagggccag ttcttgcagg aaaggatagt aggcgctttt cagggtaatt
    13201 tgcagctctg ttttactgag tgctttaaca tcaacaatct ggtttgccag ctccagccag
    13261 gcgtgacgtt gacggttatc gagcactgcg cggaagtttt ctgccgccgc ctcggcatcg
    13321 aacggttcac cgttggagaa tttcacgtca tcacgcaggg tgaaggtcca ggttttacca
    13381 tcttctgaat gagtccagct ttttgccagc cacgggatca ccgaaccgtc tgcctgatat
    13441 ttcaccaatg gttcataaac catgctctgg gcgaacatct ggttaggcgt gtaaaggtgc
    13501 gggtttagtg gcccgacatt caccggccag gcggtagtga tttcatctgg tgcagcggca
    13561 tggacgataa aagacgcaca agccagcagc gcaaatagag tgcggcggag tgtggagagc
    13621 atggtaaccc caatggatta aaatagatgg cggaaataag tatgacgatt ttaagtattc
    13681 gtcatactga ttacctgttc tggatcaata gatgggcagt cgggtgggcg gatcagttaa
    13741 ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa gggggtgggg gtgcagatcg
    13801 caaggcttaa attttctagc tgacaatggc tgactgacag cgaggagtga taggtgctat
    13861 ctacgctgac gatttgccag gcgctgccgc cacgctgttt aacgatggct tctttgcgcg
    13921 tccagatgcg ccagaacatt tccagttgct gatcaggatg cacggcgtcc atttcagcgt
    13981 gttccccgag gctgaacacg gcgttcgcca gccagcgcca gttggcgcgc gggcgaatca
    14041 cttcgatatc gcagccgact tcgccttcat cgctcaacag cagagcgata tcgtcaccgc
    14101 tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa tgcaggtttg ccttgttcgc
    14161 catagatgat ctccggtagc ggggaaagcg tgtgcgaaag caatgcacgc cccgccagcc
    14221 agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa acccggtggc agtggagctg
    14281 cgcttaaggt cgaaactttc cccagaacta tccgatacat atcagggcca acgtttaatg
    14341 gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc cggtaaccga cttttcggtc
    14401 tgcctggccc cagtaaaatc gccagtttgc taccgccttt ggtggtttcc atccagattt
    14461 tacacacact ggttaatggc accgaaagta gcatccctac cgggccgagc agccatcccc
    14521 aaattaacaa tgaaagaaat accaccatgg tggacatccc caggcgatgg cccatcatcc
    14581 gtggttctaa aatattgccg atgaccatat ggaccactaa aaacaatgcg ccgaccagaa
    14641 tacattcgta aacaccatta aacagcagca cctgaatcat tggcggtacg gcggaaatta
    14701 ccgcgccgat attgggcacg tagttgagca aaaacgccag tactgcccac atcagcgcaa
    14761 actgcacccc catcagctcc agccccagcc agacgatgac acctgtccat aaactgagta
    14821 gcgtcttcaa tgcaagatag tgcgaaacgc ctttaagtgc gcggtgtaat cctgcgatgt
    14881 gaatctgtgg gttattcagc gcaaaacgca ttttgtaagg gacgtggcgc acttcaaaca
    14941 gcataaaaac tacggtcatc accagcaaaa gcacgctcgc cattgccccg gaaagcccgg
    15001 tcattagcgc tgtggtgaag gtaaccactt tttccgagtc catccgctgc agcattcgct
    15061 ccggcgacat atgcaaatta agaaaaggca acatctcctg caatttaaaa agtttgcgcg
    15121 tcagctcctt attaaacttc ggcagcatag agataaattc gttaaacgat gccgccagta
    15181 cgccgaccag cgcggttagt gcgatcagca tcaccaccac tacaatcgta atggcaacgg
    15241 ggcgttgtac tccccgacga ataaaccagg taacgagcgg gttgaggacg atggcaaaaa
    15301 acagcgccag taacagctgc acaatgatat ctgccgctgc gtgaatgccc gcgaggatca
    15361 ctaccagcga ggccagcttg agcagaatgt gcatgcccgt tttatcgggt tgagggattt
    15421 ccattggggc ttccttgtga ctttttgtat taagtgtagc gggagtcacg ccagcaatat
    15481 tctgattctt actgctgatt ttcacgtcag cacgtggtaa aaatgaaaca ctgttgtaaa
    15541 aatgtggtga tcctcatgcc cgaacccgta gccgaacccg cgctaaacgg attgcgcctg
    15601 aatttgcgca ttgtctccat tgtcatgttt aacttcgcca gctacctcac catcgggttg
    15661 ccgctcgctg tattaccggg ctatgtccat gatgtgatgg gctttagcgc tttctgggca
    15721 ggattggtta tcagcctgca atatttcgcc accttgctga gccgtcctca tgccggacgt
    15781 tacgccgatt tgctgggccc caaaaagatt gtcgtcttcg gtttatgcgg ctgctttttg
    15841 agcggtctgg gatatctgac ggcaggatta accgccagtc tgcccgtcat cagcctgtta
    15901 ttactttgcc tgggacgcgt gatccttggg attgggcaaa gttttgccgg aacgggatcg
    15961 accctgtggg gcgttggcgt ggttggctcg ctgcatatcg ggcgagtgat ttcgtggaac
    16021 ggcattgtca attacggggc gatggcgatg ggtgcgccgt taggcgtcgt gttttatcac
    16081 tggggcggct tgcaggcgtt agcgttaatc attatgggcg tggcgctggt ggccattttg
    16141 ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca aaccactgcc gtttcgcgcg
    16201 gtgcttgggc gcgtctggct gtacggtatg gcactggcac tggcttctgc cggatttggc
    16261 gtcatcgcca cctttatcac gctgttttat gacgctaaag gttgggacgg tgcggctttc
    16321 gcgctgacgc tgtttagctg tgcgtttgtc ggtacgcgtt tgttattccc taacggcatt
    16381 aaccgtatcg gcggcttaaa cgtggcgatg atttgcttta gcgttgagat aatcggcctg
    16441 ctactggttg gcgtggcgac tatgccgtgg atggcgaaag tcggcgtctt actggcgggg
    16501 gcagggtttt cgctggtgtt cccggcattg ggcgtagtgg cggtaaaagc ggttccgcag
    16561 caaaatcagg gggcggcgct ggcaacttac accgtattta tggatttatc gcttggcgtg
    16621 accggaccac tggctgggct ggtgatgagc tgggcgggcg taccggtgat ttatctggcg
    16681 gcggcgggac tggtcgcaat cgcgttatta ctgacgtggc gattaaaaaa acggcctccg
    16741 gaacacgtcc ctgaggccgc ctcatcatct taaaacttac tgaataacca gcgtattaat
    16801 gatgttttct gcggtggtct gcgctttttg ctgatcgtca gctggcagag taatttgcat
    16861 ggtcagcagt tgattaccca cgttacccag aataacggaa gagtacgccg tctggccttt
    16921 cgcggagata atactgtcta actgctgcat tttgtgacct ttcagctcaa tggctttgtt
    16981 ggttaccact tgcagctgcg gatcgcggct acgttgctga tcttccagac gcttcgccaa
    17041 caccgccaga tcttctttcg gatcatcgcc catgatgacg atgactgctt tctgcccggt
    17101 ggcgtcggac cagacatgca tgttattggc ctgcgttccc agcttaccgc tctggtcggt
    17161 catatccgct ggcagcgaga aacttaactt gccatcaagc aggttgacgg gattcccggt
    17221 agcgttactt tccgcgaccg aaccctgcgc cgtagcgtta gtgtctttat catcacaggc
    17281 cgcaagcccc ataaccagca ggccaattcc gacatattta accagattgc gcattgactt
    17341 cttcctttcg ataaacggcc ataacggctc attcatccat cttatcacaa ctctgataac
    17401 gaacctttaa ctcgcctgca aagcgttgat ttcggattta tctgccagtc ttttcaacag
    17461 aatattgagt aatacgccat acattggcag gaagaaaacg atactgatta acactttgaa
    17521 acagtaatcg accagcgcga tttccatcca gtgttcagcc ataaaggcat ccgggctacg
    17581 ccagaaggca atgaagaaaa aggccagcgt gtcgctgacg ttaccgaaca gtgtggacgc
    17641 tgtcggtgcc agccaccagc ggcgactctg acgcaggcgg ttaaaaacgt gcacgtcgag
    17701 gatctgtccc agcgcgtagg ccatgaaact ggcggtggcg atacgggcga caaacaggtt
    17761 gaagtgggcg agtgcgccga atccctgcca ggaacccata tagaatagcg acgagatgac
    17821 gtaggagatc agcaacgccg ggatcatcac cgcaaagatg atacgacggg ccagcggtgc
    17881 gccaaaaata cgcacggtca ggtcggtagc aaggaaaata aacgggaagc taaacgcgcc
    17941 ccaggtggta tggaaaccca aaatggagac gggaagctga accagatagt tactggaggt
    18001 gatcaccagc agatgaaata acgataacca gaacaacgcc ttatagcgtt gagtttgcga
    18061 gaaaacgttc atattgtacc tttttgatta accattgggg tgagggaacc caatacgtac
    18121 gacacgtctg ttaccttaac ataataacgt gcttaccctt ttttcgagcc gccgcatgat
    18181 actgctttac gttgacaatg caatggttaa ttttcacgca atcgttaacc tggtttgctt
    18241 acggactcac agggcgtaaa ctagcgccgt ttttttatgt gatgagaaga aaatgaccga
    18301 tctcttttcc agccctgacc acacactcga cgcacttggc ctgcgctgcc cggaacccgt
    18361 aatgatggtg cgcaaaactg tgcgcaatat gcagcctggc gaaacgttgc tgattatcgc
    18421 cgacgatccg gccactaccc gcgatattcc tgggttttgt acctttatgg aacacgaact
    18481 ggttgcgaaa gagacggatg gactgcctta tcgttatttg attcgtaaag gcggttgatg
    18541 gggaaagatt ggcttcgatg ccgccttttc ccctcaccct aaccctctcc ccagaggggc
    18601 gaggggaccg atcgcgctca atgttgcgat cggtttgcct tatctcctgc gcaacaatct
    18661 taacgcattc gctgtcacca gcaccgtcgc acccgtatct gccagcaccg ccagccacag
    18721 gccggtcatc cctaacagcg tggtgacgag gaagatccct ttcagcccca gcgcaatggt
    18781 gatgttctgg cggatattgg cgtgagtggc gcgtgccagt tcaatcattt gcaccaggcc
    18841 gcgcaggtgg ttatgggtta atgctgcatc agcggtttcc agcgccacgt ctgtgccact
    18901 gcccattgca atcccaatgg cggcagcttt catcgctggc gcgtcgttaa taccatcacc
    18961 gaccatcgcc aggggcgcat gttgattcag cttggtcacc gctttgactt tatcttccgg
    19021 caataggccc gctttaaact ccagccccag ctccccggcg attgccgccg ctgcacgtgg
    19081 attatcgccg gtgaggatca ctcctttgac ccccagcgcg ttcagttcac tgatggcagt
    19141 tgcagcatcg gcgcgcaggg tatcctgcaa tgcaatgata cccagcacgt catcattacg
    19201 tactaccagc acaaccgttt gcccggcgct ttccagttca ttaatcaaac cagcaaatgc
    19261 atcagcggga tgtttcccgg cagcgcaaat caatacgcgc tcaccgttaa cctgcgcttc
    19321 aatgccagac ccgaccagcg cccgctgtga ttcggcggtg ggaatggcga gttcagcaac
    19381 ctgtgcttcg cgtacgatgg cttgcgccag tggatgcgtc gcgccttgct cgaccgccgc
    19441 cgccagtgtc agcagttcag attcactaat acccgttgcc ggatgaatcg cggtaacgcg
    19501 cggtttaccg acggtcagcg taccggtttt atcaaacgcc acctgagtaa cacgacctag
    19561 ttgttccagc gccgctccgc ctttaatcaa cgccccacga cgcgctgccg ccgccagccc
    19621 ggaggtaatc gccgcaggcg ttgagataac taacgcacac gggcagccaa tcagcagcag
    19681 cgtcagccct ttataaatcc actcctgcca gctggcggca aacagcagcg gtggcaccag
    19741 cgtcaccagc agagcgacgg ccataatcgc gggcgtatag atacggctga aacggtcgat
    19801 aaaccgctca atgggggcgc gacgctcttc ggcttcttcg atcagtttca gaatccggtc
    19861 aatggcgctg gcacccggtt ctgacagcac ttccagcgtt accagacggt ctacgctggt
    19921 ggctcctgca ggaactttat cgcccgtcgc gcgctccacc ggaatggatt ctccggtcag
    19981 ggcgctttca tcaaaactgg caaacggtga gagcagttta ccgtcggcag gcaaacgccc
    20041 acctgcggcg acttcaatca catcgccagg ccgcaggctg ttaatcgcca cctcttcccg
    20101 ctcaccgtta cgcaggcgcg tggcggtttc tggtttcagc gccattaacg cgctaacgcc
    20161 ctgacgcgcg cggctggcgg cccagccttc cagtcgttca ccaatcaaaa acagcagcaa
    20221 caccatcgca gcttcagccg ttgcgccaat aaacagtgca ccaatagcgg ctacgctcat
    20281 taaggtttca atggcgaagt agctgccgga tttgatcagc cgtaatgcct gacgagcaat
    20341 cgggtacagc ccaaccagcg tggtcgcgat aaacgccagt tgcccgaacg gatgattgaa
    20401 ctgctccaga ccccagctga ttgccatcat cacgattagt gtaatcagcg gcagattctc
    20461 tttcaggcgc gatgcttgtg gctcgtcggc ggcctgctca tcgcgcaggg aatagcccgc
    20521 tttttgcacc gcagattcaa cttgtgcacg aatgtcattg tcggcatcaa ccaccagttt
    20581 ttcggtggcg aacaacacct gcacctgatt cacacctgca agctggcgca cggcattttc
    20641 taccttgcgc gcacaggcgg cgcagtccat gccactgact ttccagctat agcgggtgcc
    20701 ggagacgttt tcagagagcg ttggcgagct ggagcatgcg ccgtcgcagc aacagtcgtt
    20761 ggcgttctgt accgtggtta gcggtttgaa cgcagcaaat tgaggggctt tcttgccgtg
    20821 attgtcaggt gtcgacatgg catactccgg ttaagttttt tctcattaac cgaaggatac
    20881 actctggagt cgactccaga gtcaagtttt atcagagata cagtgagcgg atgatgagga
    20941 agtgaccggc aaagtagcag gccgcagcaa tcgcgttatc cgcacggaag cgtcggcgat
    21001 agtggctccc cagccagaca aagttactga taaacagcaa cgaagcgcca acaaacgcag
    21061 agagcgccgg agcggtcgga cggaagaacc acagttcacc tgccagccac accatcacca
    21121 gcgtcatgcc gataaacgtg cagataggcc aacgatactc ttccagccgc gtccagataa
    21181 tcgccagtaa cagcgcaccc agaaccagca acaccagtgg cagaggccag aagaaagaga
    21241 gcgtcatctg actggcgaaa tagatggtgt acagcaggtg cgagaggaaa aacgcgccga
    21301 tggcgtacat cagacgttga cgtggcaaca gggttagcgc atcgcccagc agtgaggcgc
    21361 acagccctgc cagcaccaga tagctaatgg cgtcgaacat cggcgcttgc caggccagta
    21421 acagcaggag aagaagggtt aacggtttaa acacccagcg ttgccaggtt ggcccacgat
    21481 acgatgcatc cacagatagc catgcggaaa gacagacagc gataaacgac caaagcatct
    21541 tagttccttg aattcgttat tttttcgtaa gcggaatgcc tacggtcttg cttccagttt
    21601 agtggcgagg gtggacggat gacaacacca taactggcag ggtatgctta tttccgcatt
    21661 ttccgatgag ggataaagat gagcaaacca cctcttttct ttattgttat cattggctta
    21721 attgtcgtcg ccgcatcgtt tcgttttatg caacagcgac gggaaaaagc tgataatgat
    21781 atggctccgc tccagcaaaa gctggtggtg gtgagcaaca agcgggaaaa accgattaac
    21841 gatcgccgtt cgcgccagca ggaagtgact ccggcaggca ccagtatgcg ctacgaggca
    21901 agcttcaaac cgcaaagcgg aggaatggag cagacgtttc gcctcgacgc ccagcagtac
    21961 cacgccctga cagtgggcga taaaggtacg ctgagctata aaggaacgcg ctttgttagc
    22021 tttgttggcg aacaataagg ttatttttta actttaaatt tcttctgcca gaccagcagt
    22081 tcaaacacgc cgaaaaggaa aatccgtacc ttttcggcgg tggtcatctg cgggccatct
    22141 ttcggtaaag tggatttcaa tagcgccagc tgcataccat gcatcagcac ggtaaaaatc
    22201 agcgccacgt taacgaaaat atttagcggg cgcgggaagg gatgcaccag attgaggatt
    22261 aaaaatcccc aaacgcagag cattaacaaa cgaccaatat taatcagcat cactttctcc
    22321 ttgtgcttcg cgttgataca gccgataagc cacctgaccc gccacttttt cccgatgtaa
    22381 cgaccagttt gccggaacgg tgggcagacc gttttcgact tcgctttcga cataaatcag
    22441 ggcttcgtca gccagccagc cgttatcttc cagtaaattt atcgtctctt ctaacaagcc
    22501 acggcggaac ggtggatcga caaacacgat attatgcggt gtgccttttt gcgccaggaa
    22561 tgacatcgcg ttgctgttca ccacgcgtgc attgcctgct tttagtgtcg ccagattctt
    22621 aattaactgc tgagaaactg cacgatccat ctcaattaac gtggcccccg cagcgtagcg
    22681 cgataacgct tccagcccca gcgcgccgct cccggcgaag caatccagac attgggcgtc
    22741 aacaatgacc ggagccagcc agttaaacaa cgtttcgcgt acgcggtcgg tggtggggcg
    22801 cagacctggg ctatcaggaa ccgggagttt acggcctcgc cactgcccgc caataatgcg
    22861 gatttggccg ctgccggaat gattcggttt tttcatgata aattgctcaa tccgccagat
    22921 aacacatact tgcaggcggt gatgtgaatt aagttaagtg atagactatt tcatcatttt
    22981 tttagctgct atgtacatag cgttaacgct gtgccatgaa gcaacagcga ggagtgtagt
    23041 cgcaaatggc gaaagaaaaa aaacgtggct ttttttcctg gctgggcttt ggtcaaaaag
    23101 agcagacccc ggaaaaagag acagaagttc agaatgaaca accggttgta gaagaaatcg
    23161 ttcaggcgca agagcctgtg aaggcttcgg aacaagccgt tgaagagcag ccgcaggcgc
    23221 atactgaagc cgaggcggaa acttttgctg ccgacgttgt ggaagtcact gaacaggttg
    23281 ttgaaagtga aaaagcgcag cctgaagcgg aagttgttgc acagccggaa ccggtcgtag
    23341 aagaaacgcc ggagccagtg gctatcgaac gtgaagagct gccgttgccg gaagacgtca
    23401 acgccgaaga ggtttcgcca gaagagtggc aggctgaagc ggaaaccgta gagattgtca
    23461 aagcggcgga agaagaagcg gctaaagaag aaatcaccga cgaagagctg gaagcacagg
    23521 cgctggcagc cgaagcagca gaagaggcgg tgatggtggt tcctccggca gaagaagagc
    23581 agccggtgga agaaatcgct caggagcagg aaaaaccaac caaagaaggt ttcttcgcgc
    23641 gcctgaaacg cagcctgtta aaaaccaaag aaaatctcgg ttccggattt atcagcctgt
    23701 tccgcggtaa aaaaatcgac gatgatctgt ttgaggagct ggaagagcag cttttgatcg
    23761 ccgatgtggg cgtggaaacc acacgtaaaa ttatcaccaa tctgacggaa ggcgcatccc
    23821 gcaagcagct tcgtgatgcc gaggcgctct atggcctgct gaaagaagag atgggtgaga
    23881 ttctggcgaa agtcgatgag ccgctgaatg ttgaaggtaa aacgccgttc gtgatcctga
    23941 tggtgggcgt caacggtgtg ggtaaaacca cgacgattgg taagctggca cgtcagtttg
    24001 agcagcaggg taaatcggtg atgctggcgg cgggcgatac tttccgtgca gcagcggttg
    24061 aacagcttca ggtctggggt cagcgcaaca atattccggt gattgcccag cataccgggg
    24121 cggattccgc ctctgttatc ttcgacgcca ttcaggcagc taaagcgcgt aatatcgacg
    24181 tcctgattgc cgataccgcc ggacgcctgc agaacaaatc gcacctgatg gaagagttga
    24241 agaaaatcgt ccgcgtgatg aagaaactcg acgttgaagc gccgcatgaa gttatgctga
    24301 ctattgatgc cagcaccggg cagaacgcgg taagccaggc caaactgttc cacgaagctg
    24361 ttggcttaac cggcatcacg ctaacgaaac tggacggcac ggcgaaaggc ggggtaattt
    24421 tctcggtggc tgaccagttt ggtatcccta tccgctacat tggtgtcggc gaacgtattg
    24481 aggatttgcg tccgtttaag gcggacgact ttatagaggc actttttgcc cgagaggatt
    24541 aacaatgatt cgctttgaac atgtcagcaa ggcttatctc ggtgggagac aggcgctgca
    24601 gggcgttacg ttccatatgc agccgggtga gatggcgttt ctgaccggtc attccggcgc
    24661 agggaaaagt accctcctga agctgatctg tgggattgag cggcccagcg ccgggaaaat
    24721 ctggtttagc ggccatgaca tcacgcgtct gaaaaaccgt gaagttccgt ttctgcgccg
    24781 ccagattggc atgattttcc aggatcacca tctactgatg gaccgtactg tctacgataa
    24841 cgtggcgatc ccgctgatta tcgctggtgc cagcggtgac gatattcgtc gccgggtgtc
    24901 ggcggcactg gataaagtcg ggctactgga caaagcgaag aacttcccta ttcagctttc
    24961 cggcggtgaa caacagcgtg ttggcattgc ccgcgcggtg gtgaataagc ccgcggtact
    25021 gctggcggac gaaccgaccg gtaacctgga cgacgcgttg tcggaaggga ttctacgtct
    25081 gtttgaagag tttaaccgcg ttggggtaac cgtattgatg gcaacgcacg acatcaacct
    25141 gatttcacgg cgttcccatc gcatgctcac cctgagcgat ggtcacttgc atggaggcgt
    25201 gggccatgaa taagcgcgat gcaatcaatc atattcggca gtttggtggg cgtctggatc
    25261 gcttccgtaa atcggtcggc ggctcaggcg acggcggtcg taacgcaccg aaacgcgcga
    25321 aatcctcgcc aaaaccggta aatcgcaaaa ccaacgtttt caacgaacag gtgcgctatg
    25381 ccttccacgg cgcattgcag gatctgaaaa gcaaaccgtt cgccacgttt ttaacggtga
    25441 tggttatcgc catctccctg acgctgccca gcgtctgtta tatggtgtac aaaaacgtta
    25501 atcaggcggc gacgcagtat tatccgtcac cgcaaatcac tgtttacttg caaaaaacgc
    25561 tggacgatga cgccgctgcg ggcgtggtgg cacagttgca ggccgagcaa ggcgtggaga
    25621 aagtgaacta tctttctcgt gaagacgcac tgggcgagtt ccgtaactgg tctggttttg
    25681 gtggtgcgct ggatatgctg gaagaaaacc cgcttccggc agtggcggtg gtgatcccga
    25741 aactcgattt tcaggggacg gaatcactca atacgctgcg tgatcgtatc acgcagatta
    25801 acggcattga cgaagtgcgg atggatgaca gctggtttgc ccgtctggcg gcgttgaccg
    25861 ggctggtcgg gcgcgtttcg gcgatgatcg gcgtgttgat ggtggcggca gtgttcctcg
    25921 tcatcggcaa cagcgtacgt ctgagcatct ttgctcgccg tgactccatc aacgtgcaga
    25981 aactgattgg tgcgacggat ggtttcatcc ttcgcccgtt cctgtatggt ggcgcactgc
    26041 tgggattttc tggcgcattg ttgtcattaa ttttgtcaga aattctggtg ctgcgattgt
    26101 catcggcggt tgcggaagtg gcacaggttt tcggaacgaa gtttgatatc aatgatttat
    26161 cgttcgatga atgcctgcta ttgctgctgg tatgctcgat gattggctgg gtggcagcgt
    26221 ggcttgccac ggtacaacat ttacgccact ttacgcctga ataataaaag cgtgttatac
    26281 tctttccctg caatgggttc cgtagcaggg aaagagatcc cgttgtctct tcccggtatt
    26341 tcatctctat gtcacatttt gtgcgtaatt tattcacaag cttgcattga acttgtggat
    26401 aaaatcactg tctgataaaa cagtggatga taacctcgtt gctcttaagc tctggcacag
    26461 ttgttgctac cactgaagcg ccagaagata tcgattgaga ggatttgaat gactgacaaa
    26521 atgcaaagtt tagctttagc cccagttggc aacctggatt cctacatccg ggcagctaac
    26581 gcgtggccga tgttgtcggc tgacgaggag cgggcgctgg ctgaaaagct gcattaccat
    26641 ggcgatctgg aagcagctaa aacgctgatc ctgtctcacc tgcggtttgt tgttcatatt
    26701 gctcgtaatt atgcgggcta tggcctgcca caggcggatt tgattcagga aggtaacatc
    26761 ggcctgatga aagcagtgcg ccgtttcaac ccggaagtgg gtgtgcgcct ggtctccttc
    26821 gccgttcact ggatcaaagc agagatccac gaatacgttc tgcgtaactg gcgtatcgtc
    26881 aaagttgcga ccaccaaagc gcagcgcaaa ctgttcttca acctgcgtaa aaccaagcag
    26941 cgtctgggct ggtttaacca ggatgaagtc gaaatggtgg cccgtgaact gggcgtaacc
    27001 agcaaagacg ttcgtgagat ggaatcacgt atggcggcac aggacatgac ctttgacctg
    27061 tcttccgacg acgattccga cagccagccg atggcaccgg tgctctatct gcaggataaa
    27121 tcatctaact ttgccgacgg catcgaagat gataactggg aagagcaggc ggcaaaccgt
    27181 ctgaccgacg cgatgcaagg tctggacgaa cgcagccagg acatcatccg cgcgcgctgg
    27241 ctggacgaag acaacaagtc cacgttgcag gaactggctg accgttacgg tgtttccgct
    27301 gaacgtgtac gccagctgga aaagaacgcg atgaaaaaac tgcgcgctgc tatagaagcg
    27361 taatttccgc tattaagcag agaaccctgg atgcgagtcc ggggtttttg ttttttgggc
    27421 ctctacaata atcaatttcc cctccggcaa aacgccaatc cccacgcaga ttgttaataa
    27481 actgtcaaaa tagctattcc aatatcataa aaatcgggta tgttttagca gagtatgctg
    27541 ctaaagcacg ggtagccatg cataaaacga aataaagtgc tgaacaacaa catcacaaca
    27601 cacgtaataa ccagaagaat ggggattctc aggatgaaca taaagggtaa agcgttactg
    27661 gcaggatgta tcgcgctggc attcagcaat atggctctgg cagaagatat taaagtcgcg
    27721 gtcgtgggcg caatgtccgg tccggttgcg cagtacggtg accaagagtt taccggcgca
    27781 gagcaggcgg ttgcggatat caacgctaaa ggcggcatta aaggcaacaa actgcaaatc
    27841 gtaaaatatg acgatgcctg tgacccgaaa caggcggttg cggtggcgaa caaagtcgtt
    27901 aacgacggca ttaaatatgt gattggtcac ctctgttctt catcaacgca gcctgcgtct
    27961 gacatctacg aagacgaagg cattttaatg atcaccccag cggcaaccgc gccggagctg
    28021 accgcccgtg gctatcagct gatcctgcgc accaccggcc tggactccga ccaggggccg
    28081 acggcggcga aatatattct tgagaaagtg aaaccgcagc gtattgctat cgttcacgac
    28141 aaacagcaat acggcgaagg tctggcacga gcggtgcagg acggcctgaa gaaaggcaat
    28201 gctaacgtgg tgttctttga tggtatcacc gccggggaaa aagatttctc tacgctggtg
    28261 gcgcgtctga agaaagagaa tatcgacttc gtttactacg gcggttacca cccggaaatg
    28321 gggcaaatcc tgcgtcaggc acgcgcggca gggctgaaaa ctcagtttat ggggccggaa
    28381 ggtgtggcta acgtttcgct gtctaacatt gcgggcgaat cagcggaagg gctgctggtg
    28441 accaagccga agaactacga tcaggttccg gcaaacaaac ccattgttga cgcgatcaaa
    28501 gcgaaaaaac aggacccaag cggcgcattc gtttggacca cctacgccgc gctgcaatct
    28561 ttgcaggcgg gcctgaatca gtctgacgat ccggctgaaa tcgccaaata cctgaaagcg
    28621 aactccgtgg ataccgtaat gggaccgctg acgtgggatg agaaaggcga tctgaaaggc
    28681 tttgagttcg gcgtatttga ctggcacgcc aacggcacgg cgacagatgc gaagtaatca
    28741 ttaatcggca actttgggtt gccgccaaat tgctaatatc gagtacgttg cttcatgccg
    28801 gatgcggcgt aaacgcctta tccggcctat aagatccaaa gaaatcagta aattgcaaca
    28861 cacattgtag gcctgataag cgtagcgcat caggcaattt ctcgttggtc atcacttttg
    28921 aaatcaactt gacgattaac gcttctccca gccgccctgt tgcgccgtaa atcccagtgc
    28981 ctgcataaac gctgtcatca caccgcgatc ttccacgcct gcatccgcca tccaccagca
    29041 tgaaacgcca ggattgttac gcaaaacctc ttccagcaga tattgcccca caccgcgacg
    29101 tcgggtgact tcccgcacac gcagggaatc cagtgctccc tcggtgccgc ttaaggttac
    29161 ccgtacggca gcgagcaggc gctcgttaaa acgcgcggcg tagatacggt ggttatcgtc
    29221 aacctgtaaa gaggaagggg aatactccgg ccagatcttt tgcaggtcaa tccggtcctg
    29281 gtcgctaaag ttttctaatc gaatgatggt cagcttcatg ggtaacccgt gtaaatcaca
    29341 aaagtggaac cagtgtagcg aaataattta at
//
LOCUS       DAZOXR010000036        29131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZOXR010000036 DAZOXR010000000
VERSION     DAZOXR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.036"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(82..1365)
                     /gene="gltA"
                     /locus_tag="ACHUNN_001350"
     CDS             complement(82..1365)
                     /gene="gltA"
                     /locus_tag="ACHUNN_001350"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0575773.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1571..1678)
                     /locus_tag="ACHUNN_001351"
     CDS             complement(1571..1678)
                     /locus_tag="ACHUNN_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575774.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2059..2463
                     /gene="sdhC"
                     /locus_tag="ACHUNN_001352"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2059..2463
                     /gene="sdhC"
                     /locus_tag="ACHUNN_001352"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0575775.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2457..2804
                     /gene="sdhD"
                     /locus_tag="ACHUNN_001353"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2457..2804
                     /gene="sdhD"
                     /locus_tag="ACHUNN_001353"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0575776.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2804..4570
                     /gene="sdhA"
                     /locus_tag="ACHUNN_001354"
     CDS             2804..4570
                     /gene="sdhA"
                     /locus_tag="ACHUNN_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0575777.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4586..5302
                     /gene="sdhB"
                     /locus_tag="ACHUNN_001355"
     CDS             4586..5302
                     /gene="sdhB"
                     /locus_tag="ACHUNN_001355"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0575778.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5603..8404
                     /gene="sucA"
                     /locus_tag="ACHUNN_001356"
     CDS             5603..8404
                     /gene="sucA"
                     /locus_tag="ACHUNN_001356"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0575779.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8419..9636
                     /gene="odhB"
                     /locus_tag="ACHUNN_001357"
     CDS             8419..9636
                     /gene="odhB"
                     /locus_tag="ACHUNN_001357"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0575780.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9730..10896
                     /gene="sucC"
                     /locus_tag="ACHUNN_001358"
     CDS             9730..10896
                     /gene="sucC"
                     /locus_tag="ACHUNN_001358"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0575781.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10896..11765
                     /gene="sucD"
                     /locus_tag="ACHUNN_001359"
     CDS             10896..11765
                     /gene="sucD"
                     /locus_tag="ACHUNN_001359"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0575782.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11869..12591)
                     /gene="mngR"
                     /locus_tag="ACHUNN_001360"
     CDS             complement(11869..12591)
                     /gene="mngR"
                     /locus_tag="ACHUNN_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0575783.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12760..14676
                     /gene="mngA"
                     /locus_tag="ACHUNN_001361"
     CDS             12760..14676
                     /gene="mngA"
                     /locus_tag="ACHUNN_001361"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0575784.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14694..17327
                     /gene="mngB"
                     /locus_tag="ACHUNN_001362"
     CDS             14694..17327
                     /gene="mngB"
                     /locus_tag="ACHUNN_001362"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR0575785.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17352..17579
                     /locus_tag="ACHUNN_001363"
     CDS             17352..17579
                     /locus_tag="ACHUNN_001363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575786.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            18175..19743
                     /gene="cydA"
                     /locus_tag="ACHUNN_001364"
     CDS             18175..19743
                     /gene="cydA"
                     /locus_tag="ACHUNN_001364"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0575787.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            19759..20898
                     /gene="cydB"
                     /locus_tag="ACHUNN_001365"
     CDS             19759..20898
                     /gene="cydB"
                     /locus_tag="ACHUNN_001365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0575788.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            20913..21026
                     /gene="cydX"
                     /locus_tag="ACHUNN_001366"
     CDS             20913..21026
                     /gene="cydX"
                     /locus_tag="ACHUNN_001366"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0575789.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            21026..21319
                     /gene="ybgE"
                     /locus_tag="ACHUNN_001367"
     CDS             21026..21319
                     /gene="ybgE"
                     /locus_tag="ACHUNN_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0575790.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            21469..21873
                     /gene="ybgC"
                     /locus_tag="ACHUNN_001368"
     CDS             21469..21873
                     /gene="ybgC"
                     /locus_tag="ACHUNN_001368"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0575791.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            21870..22562
                     /gene="tolQ"
                     /locus_tag="ACHUNN_001369"
     CDS             21870..22562
                     /gene="tolQ"
                     /locus_tag="ACHUNN_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0575792.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            22566..22994
                     /gene="tolR"
                     /locus_tag="ACHUNN_001370"
     CDS             22566..22994
                     /gene="tolR"
                     /locus_tag="ACHUNN_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0575793.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            23059..24291
                     /gene="tolA"
                     /locus_tag="ACHUNN_001371"
     CDS             23059..24291
                     /gene="tolA"
                     /locus_tag="ACHUNN_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0575794.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            24424..25716
                     /gene="tolB"
                     /locus_tag="ACHUNN_001372"
     CDS             24424..25716
                     /gene="tolB"
                     /locus_tag="ACHUNN_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0575795.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            25751..26272
                     /gene="pal"
                     /locus_tag="ACHUNN_001373"
     CDS             25751..26272
                     /gene="pal"
                     /locus_tag="ACHUNN_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0575796.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            26282..27073
                     /gene="cpoB"
                     /locus_tag="ACHUNN_001374"
     CDS             26282..27073
                     /gene="cpoB"
                     /locus_tag="ACHUNN_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0575797.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            27238..27313
                     /locus_tag="ACHUNN_001375"
     tRNA            27238..27313
                     /locus_tag="ACHUNN_001375"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27271..27273,aa:Lys,seq:ttt)
     gene            27463..27538
                     /locus_tag="ACHUNN_001376"
     tRNA            27463..27538
                     /locus_tag="ACHUNN_001376"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27496..27498,aa:Val,seq:tac)
     gene            27542..27617
                     /locus_tag="ACHUNN_001377"
     tRNA            27542..27617
                     /locus_tag="ACHUNN_001377"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27575..27577,aa:Lys,seq:ttt)
     gene            27666..27741
                     /locus_tag="ACHUNN_001378"
     tRNA            27666..27741
                     /locus_tag="ACHUNN_001378"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27699..27701,aa:Lys,seq:ttt)
     gene            27775..27850
                     /locus_tag="ACHUNN_001379"
     tRNA            27775..27850
                     /locus_tag="ACHUNN_001379"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27808..27810,aa:Lys,seq:ttt)
     gene            27875..28006
                     /locus_tag="ACHUNN_001380"
     ncRNA           27875..28006
                     /ncRNA_class="other"
                     /locus_tag="ACHUNN_001380"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            28255..>29131
                     /gene="nadA"
                     /locus_tag="ACHUNN_001381"
     CDS             28255..>29131
                     /gene="nadA"
                     /locus_tag="ACHUNN_001381"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0575798.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         7123 a         7121 c         7847 g         7040 t
ORIGIN      
        1 aattttttgt gtttgggcat ataaaaatta acccgccatt tgaacggcgg gttaaaatat
       61 ttacaactta gcaatcaacc attaacgctt gatatcgctt ttaaagtcgc gtttttcata
      121 tcctgtatac agctgacgcg gacgggcaat cttcataccg tcactgtgca tttcgctcca
      181 gtgggcgatc cagccaacgg tacgtgccat tgcgaaaatg acggtgaaca tggaagacgg
      241 aatacccatc gctttcagga tgataccaga gtagaaatcg acgttcgggt acagtttctt
      301 ctcgataaag tacgggtcgt tcagcgcgat gttttccagc tccatagcca cttccagcag
      361 gtcatctttg gtgcccagct ctttcagaac ttcatggcag gtttcacgca ttacggtggc
      421 gcgcgggtcg taatttttgt acacgcggtg accgaagccc atcaggcgga aagaatcatt
      481 tttatctttc gcacgacgaa caaattccgg aatgtgttta acggagctaa tttcttccag
      541 cattttcagc gccgcttcgt tagcaccacc gtgcgcaggt ccccacagtg aagcaatacc
      601 tgctgcgata caggcaaacg ggttcgcacc cgaagagcca gcggtacgca cggtggaggt
      661 agaggcgttc tgttcatggt cagcgtgcag gatcagaata cggtccatag cacgttccag
      721 aatcggatta acttcatacg gttcgcacgg cgtggagaac atcatattca ggaagttacc
      781 ggcataggag agatcgttgc gcggataaac aaatggctga ccaatggaat acttgtaaca
      841 catcgcggcc atggtcggca ttttcgacag caggcggaac gcggcaattt cacggtgacg
      901 aggattgtta acatccagcg agtcgtgata gaacgccgcc agcgcgccgg taataccaca
      961 catgactgcc attggatgcg agtcgcgacg gaaagcatgg aacagacggg taatctgctc
     1021 gtggatcatg gtatgacggg tcaccgtagt tttaaattcg tcatactgtt cctgagtcgg
     1081 tttttcacca ttcagcagga tgtaacaaac ttccaggtag ttagaatcgg tcgccagctg
     1141 atcgatcggg aaaccgcggt gcagcaaaat accttcatca ccatcaataa aagtaatttt
     1201 agattcgcag gatgcggttg aagtgaagcc tgggtcaaag gtgaacacac cttttgaacc
     1261 gagagtacgg atatcaataa catcttgacc cagcgtgcct ttcagcacat ccagttcaac
     1321 agctgtgtcc ccgttgaggg tgagttttgc ttttgtatca gccatttaag gtctccttag
     1381 cgccttattg cgtaagactg ccggaactta aatttgcctt cgcacatcaa cctggcttta
     1441 cccgtttgtt atttggctcg ccgctctgtg aaagagggga aaacctgggt acagagctct
     1501 gggcgcttgc aggtaaagga tccattgatg acgaataaat ggcgaatcaa gtacttagca
     1561 atccgaatta ttaaacttgt ctaccactaa taactgtccc gaatgaattg gtcaatactt
     1621 ccacactgtt acataagtta atcttaggtg aaataccgac ttcataactt ttacgcatta
     1681 tatgcttttc ctggcaatgt ttgtaacaac tttgttgaat gattgtcaaa ttagatgatt
     1741 aaaaattaaa taaatgttgt tatcgtgacc tggatcactg ttcaggataa aacccgacaa
     1801 actatatgta ggttaattgt aatgattttg tgaacagcct atactgccgc caggtctccg
     1861 gaacaccctg caatcccgag ccacccagcg ttgtaacgtg tcgttttcgc atctggaagc
     1921 agtgttttgc atgacgcgca gttatagaaa ggacgctgtc tgacccgcaa gcagaccgga
     1981 ggaaggaaat cccgacgtct ccaggtaaca gaaagttaac ctctgtgccc gtagtcccca
     2041 gggaataata agaacagcat gtgggcgtta ttcatgataa gaaatgtgaa aaaacaaaga
     2101 cctgttaatc tggacctaca gaccatccgg ttccccatca cggcgatagc gtccattctc
     2161 catcgcgttt ccggtgtgat cacctttgtt gcagtgggca tcctgctgtg gcttctgggt
     2221 accagcctct cttcccctga aggtttcgag caagcttccg cgattatggg cagcttcttc
     2281 gtcaaattta tcatgtgggg catccttacc gctctggcgt atcacgtcgt cgtaggtatt
     2341 cgccacatga tgatggattt tggctatctg gaagaaacat tcgaagcggg taaacgctcc
     2401 gccaaaatct cctttgttat tactgtcgtg ctttcacttc tcgcaggagt cctcgtatgg
     2461 taagcaacgc ctccgcatta ggacgcaatg gcgtacatga tttcatcctc gttcgcgcta
     2521 ccgctatcgt cctgacgctc tacatcattt atatggtcgg ttttttcgcc accagtggcg
     2581 aactgacata tgaagtctgg atcggtttct tcgcctctgc gttcaccaaa gtgttcaccc
     2641 tgctggcgct gttttctatc ttgattcatg cctggatcgg catgtggcag gtgttgaccg
     2701 actacgttaa accgctggca ttgcgcctga tgctgcaact ggtgattgtc gttgcactgg
     2761 tggtttacgt gatttatgga ttcgttgtgg tgtggggtgt gtgatgaaat tgccagtcag
     2821 agaatttgat gcagttgtga ttggtgccgg tggcgcaggt atgcgcgcgg cgctgcaaat
     2881 ttcccagagc ggccagacct gtgcgctgct ctctaaagtc ttcccgaccc gttcccatac
     2941 cgtttctgcg caaggcggca ttaccgttgc gctgggtaat acccatgaag ataactggga
     3001 atggcatatg tacgacaccg taaaagggtc ggactatatc ggtgaccagg acgcgattga
     3061 atatatgtgt aaaaccgggc cggaagcgat tctggaactc gaacacatgg gcctgccgtt
     3121 ctcgcgtctt gatgatggtc gtatctatca acgtccgttt ggcggtcagt cgaaaaactt
     3181 cggcggcgag caggcggcac gcactgcggc ggcggctgac cgtaccggtc acgcactgtt
     3241 gcacacgctt tatcagcaga acctgaaaaa ccacaccacc attttctccg agtggtatgc
     3301 gctggatctg gtgaaaaacc aggatggcgc ggtggtgggt tgtaccgcac tgtgcatcga
     3361 aaccggtgaa gtggtttatt tcaaagcccg cgctaccgtg ctggcgactg gcggggcagg
     3421 gcgtatttat cagtccacca ctaacgccca cattaacact ggcgacggtg tcggcatggc
     3481 tatccgcgca ggcgtaccgg tgcaggatat ggaaatgtgg caattccacc cgaccggcat
     3541 tgccggtgcg ggcgtactgg tcaccgaagg ttgccgtggt gaaggcggtt atctgctgaa
     3601 caaacatggc gaacgcttta tggagcgtta tgcgccgaac gccaaagacc tggcgggccg
     3661 tgatgtggtg gcgcgttcca tcatgattga aatccgtgaa ggtcgcggct gtgatggtcc
     3721 gtgggggcca cacgcgaaac tgaaactcga tcacctgggt aaagaagttc tcgaatcccg
     3781 tctgccgggt atcctggagc tttcccgtac ctttgctcac gtcgatccgg tgaaagagcc
     3841 gattccggtt atcccaacct gtcactacat gatgggcggt attccgacca aagtgaccgg
     3901 tcaggcgctg acggtgaacg agaaaggcga agatgtggtt gttccggggc tgtttgccgt
     3961 tggtgaaatc gcttgtgtat cggtacacgg cgctaaccgt ctgggcggca actcgctgct
     4021 ggacctggtg gtctttggtc gtgcggcagg tctgcatctg caagagtcta tcgccgaaca
     4081 gggcgcattg cgcgatgcca gcgagtctga tgttgaagca tctctggatc gtctgcaccg
     4141 ctggaacaat aatcgtaacg gtgaagatcc ggtggcgatt cgtaaagcgc tgcaagaatg
     4201 tatgcagcat aacttctcgg tcttccgtga aggtgatgcg atggcgaaag ggcttgagca
     4261 gttgaaagtg atccgcgagc gtctgaaaaa tgcccgtctg gatgacactt ccagcgagtt
     4321 caacacccag cgcgttgagt gcttggaact ggataacctg atggaaacgg cgtatgcaac
     4381 ggctgtttct gccaacttcc gtaccgaaag ccgtggcgca catagccgct tcgacttccc
     4441 ggatcgtgat gatgaaaact ggctgtgcca ctccctgtat ctgccagagt cggaatccat
     4501 gacgcgccga agcgtcaaca tggaaccgaa actgcgcccg gcattcccgc cgaagattcg
     4561 tacttactaa tgcggagaca ggaaaatgag actcgagttt tcaatttatc gctataaccc
     4621 ggatgttgat gatgctccgc gtatgcagga ttaccccctg gaagcggaag aaggtcgcga
     4681 catgatgctg ctggatgcgc ttatccagct gaaagagaaa gatcccagcc tgtcgttccg
     4741 ccgctcctgc cgtgaaggtg tgtgcggttc cgacggtctg aacatgaacg gcaagaatgg
     4801 tctggcctgt attaccccga tttcggcact caaccagccg ggcaagaaga ttgtgattcg
     4861 cccgctgcca ggtttaccgg tgatccgcga tttggtggta gacatgggac aattctatgc
     4921 gcaatatgag aaaattaagc cttacctgtt gaataatgga caaaatccgc cagctcgcga
     4981 gcatttacag atgccagagc agcgcgaaaa actcgacggg ctgtatgaat gtattctctg
     5041 cgcatgttgt tcaacctctt gtccgtcttt ctggtggaat cccgataagt ttatcggccc
     5101 ggcaggcttg ttagcggcat atcgtttcct gatcgatagc cgtgataccg agactgacag
     5161 ccgcctcgac ggtttgagcg atgcattcag cgtattccgc tgtcacagca tcatgaactg
     5221 cgtcagtgta tgtccgaagg ggctgaaccc gacgcgcgcc atcggccata ttaagtcgat
     5281 gttgttgcaa cgtaatgcgt aaaccgtagg cctgataaga cgcgcaagcg tcgcatcagg
     5341 caaccagtgc cggatgcggc gtgaacgcct tatccggcct acaagtcatt acccgtaggc
     5401 ctgataagcg cagcgcatca ggcgtaacaa agaaatgcag gaaatcttta aaaactgccc
     5461 ctgacactaa gacagttttt aaaggttcct tcgcgagcca ctacatagac aagagctcgc
     5521 aagtgaaccc cggcacgcac atcactgtgc gtggtagtat ccacggcgaa gtaagcataa
     5581 aaaagatgct taagggatca cgatgcagaa cagcgctttg aaagcctggt tggactcttc
     5641 ttacctctct ggcgcaaacc agagctggat agaacagctc tatgaagact tcttaaccga
     5701 tcctgactcg gttgacgcta actggcgttc gacgttccag cagttacctg gtacgggagt
     5761 caaaccggat caattccact ctcaaacgcg tgaatatttc cgccgcctgg cgaaagacgc
     5821 ttcacgttac tcttcaacga tctccgaccc tgacaccaat gtgaagcagg ttaaagtcct
     5881 gcagctcatt aacgcatacc gcttccgtgg tcaccagcat gcgaatctcg atccgctggg
     5941 actgtggcag caagataaag tggccgatct ggatccgtct ttccacgatc tgaccgaagc
     6001 agacttccag gagaccttca acgtcggttc atttgccagc ggcaaagaaa acatgaaact
     6061 cggcgagctg ctggaagccc tcaagcaaac ctactgtggc ccgattggtg ccgagtatat
     6121 gcacattacc agcaccgaag aaaaacgctg gatccaacag cgtatcgagt ctggtcgcgc
     6181 gactttcaat agcgaagaga aaaaacgctt cttaagcgaa ctgaccgccg ctgaaggcct
     6241 tgaacgttac ctcggcgcaa aattccctgg cgcaaaacgc ttctcgctgg aaggcggtga
     6301 cgcgttaatc ccgatgctta aagagatgat ccgccacgct ggcaacagcg gcacccgcga
     6361 agtggttctt gggatggcgc accgtggtcg tctgaacgtg ctggtgaacg tgctgggtaa
     6421 aaaaccgcaa gacttgttcg acgagtttgc cggtaaacat aaagaacacc tcggcacggg
     6481 tgacgtgaaa taccacatgg gcttctcgtc tgacttccag accgatggcg gcctggtgca
     6541 cctggcgctg gcgtttaacc cgtctcacct tgagattgta agcccggtag ttatcggttc
     6601 tgttcgtgcc cgtctggaca gacttgatga gccgagcagc aacaaagtgc tgccaatcac
     6661 catccacggt gacgccgcag tgaccgggca gggcgtggtt caggaaactc tgaacatgtc
     6721 gaaagcgcgt ggttatgaag ttggcggtac ggtacgtatc gttatcaaca accaggttgg
     6781 tttcaccacc tctaatccac tggatgctcg ttctacaccg tactgtactg atatcggtaa
     6841 gatggttcag gcaccgattt tccacgttaa cgcggatgat ccggaagccg ttgcctttgt
     6901 gacccgtctg gcgctcgatt tccgtaacac ctttaaacgt gatgtcttca tcgacctggt
     6961 gtgctaccgc cgtcacggcc acaacgaagc cgacgagccg agcgcaaccc agccgctgat
     7021 gtatcagaaa atcaaaaaac atccgacacc gcgcaaaatc tacgctgaca agctggagca
     7081 ggaaaaagtg gcgacgctgg aagatgccac cgagatggtt aacctgtacc gcgatgcgct
     7141 ggatgctggc gattgcgtag tggcagagtg gcgtccgatg aacatgcact ctttcacctg
     7201 gtcgccgtac ctcaaccacg aatgggacga agagtacccg aacaaagttg agatgaagcg
     7261 cctgcaggag ctggcgaaac gcatcagcac ggtgccggaa gcagttgaaa tgcagtctcg
     7321 cgttgccaag atttatggcg atcgccaggc gatggctgcc ggtgagaaac tgttcgactg
     7381 gggcggtgcg gaaaacctcg cttacgccac gctggttgac gaaggcattc cggttcgcct
     7441 gtcgggtgaa gactccggtc gcggtacctt cttccaccgc cacgcggtga tccacaacca
     7501 gtctaacggt tccacttaca cgccgttgca acacatccat aacgggcagg gcgcgttccg
     7561 cgtctgggac tccgtactgt ctgaagaagc agtgctggcg tttgaatacg gttatgccac
     7621 cgcagaacca cgcactctga ccatctggga agcgcagttc ggtgacttcg ccaacggtgc
     7681 gcaggtggtt atcgaccagt tcatctcttc tggcgaacag aaatggggcc ggatgtgtgg
     7741 tctggtgatg ttgctgccgc acggttacga agggcagggg ccggagcact cctccgcgcg
     7801 tctggaacgt tatctgcaac tttgtgctga gcaaaacatg caggtttgcg ttccgtctac
     7861 cccggcacag gtttaccaca tgctgcgtcg tcaggcgctg cgcgggatgc gtcgtccgct
     7921 ggtagtgatg tcgccgaaat ccctgctgcg tcatccgctg gcggtttcca gcctcgaaga
     7981 actggcgaac ggcaccttcc tgccagccat cggtgaaatc gacgagcttg atccgaaggg
     8041 cgtgaagcgc gtagtgatgt gttctggtaa ggtttattac gacctgctgg aacagcgtcg
     8101 taagaacaat caacacgatg tcgccattgt gcgtatcgag caactctacc cgttcccgca
     8161 taaagcgatg caggaagtgt tgcagcagtt tgctcacgtc aaggattttg tctggtgcca
     8221 ggaagagccg ctcaaccagg gcgcatggta ctgcagccag catcatttcc gtgaagtgat
     8281 tccgtttggg gcttctctgc gttatgcagg ccgcacggcc tccgcctctc cggcggtagg
     8341 gtatatgtcc gttcaccaga aacagcaaca agatctggtt aatgacgcgc tgaacgtcga
     8401 ataaataaag gatacacaat gagtagcgta gatattctgg tccctgacct gcctgaatcc
     8461 gtagccgatg ccaccgtcgc aacctggcat aaaaaacccg gcgacgcagt cgtacgtgat
     8521 gaagtgctgg tagaaatcga aactgacaaa gtggtactgg aagtaccggc atcagcagac
     8581 ggcattctgg atgcggttct ggaagatgaa ggtacaacgg taacgtctcg tcagatcctt
     8641 ggtcgcctgc gtgaaggcaa cagcgccggt aaagaaacca gcgccaaatc tgaagagaaa
     8701 gcgtccactc cggcgcaacg ccagcaggcg tctctggaag agcaaaacaa cgatgcgtta
     8761 agcccggcga tccgtcgcct gctggctgaa cacaatctcg acgccagcgc cattaaaggc
     8821 accggtgtgg gtggtcgtct gactcgtgaa gatgtggaaa aacatctggc gaaagccccg
     8881 gcgaaagagt ctgctccggc agcggctgct ccggcggcgc aaccggctct ggctgcacgt
     8941 agtgaaaaac gtgtcccgat gactcgcctg cgtaagcgtg tggcagagcg tctgctggaa
     9001 gcgaaaaact ccaccgccat gctgaccacg ttcaacgaag tcaacatgaa gccgattatg
     9061 gatctgcgta agcagtacgg tgaagcgttt gaaaaacgcc acggcatccg tctgggcttt
     9121 atgtccttct acgtgaaagc ggtggttgaa gccctgaaac gttacccgga agtgaatgct
     9181 tctatcgacg gtgatgacgt ggtttaccac aactatttcg acgtcagcat ggcggtttcc
     9241 acgccgcgcg gcctggtgac gccggtactg cgtgatgtcg ataccctcgg catggcagac
     9301 atcgagaaga aaatcaaaga gctggcagtt aaaggccgtg acggcaagct gacggttgaa
     9361 gatctgaccg gtggtaactt caccatcacc aacggtggtg tgttcggttc cctgatgtct
     9421 acgccgatca tcaacccgcc gcagagcgca attctgggta tgcacgctat caaagatcgt
     9481 ccgatggcgg tgaatggtca ggttgagatc ctgccgatga tgtacctggc gctgtcctac
     9541 gatcaccgtc tgatcgatgg tcgcgaatcc gtgggcttcc tggtaacgat caaagagttg
     9601 ctggaagatc cgacgcgtct gctgctggac gtgtagtagt ttaagcttca cctgcactgt
     9661 agaccgacgt ggatagggtg tcggtctacg gtttaaaaga taacgattac tgaaggatgg
     9721 acagaacaca tgaacttaca tgaatatcag gcaaaacaac tttttgcccg ctatggctta
     9781 ccagcaccgg tgggttatgc ctgtactact ccgcgcgaag cagaagaagc cgcttcaaaa
     9841 atcggtgccg gcccgtgggt agtgaaatgt caggttcacg ctggtggccg cggtaaagcg
     9901 ggcggtgtga aagttgtaaa cagcaaagaa gacatccgtg cttttgcaga aaactggctc
     9961 ggcaagcgtc tggtaacgta tcaaacagat gccaatggcc aaccggttaa ccagattctg
    10021 gttgaagcag cgaccgatat cgctaaagag ctgtatctcg gtgccgttgt agaccgtagt
    10081 tcccgtcgtg tggtctttat ggcctccacc gaaggcggcg tggaaatcga aaaagtggcg
    10141 gaagaaactc cgcacctgat ccacaaagtt gcgcttgatc cgctgactgg cccgatgccg
    10201 tatcagggac gcgagctggc gttcaaactg ggtctggaag gtaaactggt tcagcagttc
    10261 accaaaatct tcatgggcct ggcgaccatt ttcctggagc gcgacctggc gttgatcgaa
    10321 atcaacccgc tggtcatcac caaacagggc gatctgattt gcctcgacgg caaactgggc
    10381 gctgacggca acgcactgtt ccgccagcct gatctgcgcg aaatgcgtga ccagtcgcag
    10441 gaagatccgc gtgaagcaca ggctgcacag tgggaactga actacgttgc gctggacggt
    10501 aacatcggtt gtatggttaa cggcgcaggt ctggcgatgg gtacgatgga catcgttaaa
    10561 ctgcacggcg gcgaaccggc taacttcctt gacgttggcg gcggcgcaac caaagaacgt
    10621 gtaaccgaag cgttcaaaat catcctctct gacgacaaag tgaaagccgt tctggttaac
    10681 atcttcggcg gtatcgttcg ttgcgacctg atcgctgacg gtatcatcgg cgcggtagca
    10741 gaagtgggtg ttaacgtacc ggtcgtggta cgtctggaag gtaacaacgc cgaactcggc
    10801 gcgaagaaac tggctgacag cggcctgaat attattgcag caaaaggtct gacggatgca
    10861 gctcagcagg ttgttgccgc agtggagggg aaataatgtc cattttaatt gataaaaaca
    10921 ccaaggttat ctgccagggc tttactggta gccaggggac tttccactca gaacaggcca
    10981 ttgcatacgg cactaaaatg gttggcggcg taaccccagg taaaggcggt actacccacc
    11041 tcggcctgcc ggtgttcaac accgtgcgtg aagctgttgc tgctactggc gctaccgctt
    11101 ctgttatcta cgtaccagca ccgttctgca aagactccat tctggaagct attgatgcag
    11161 gcatcaaact gattatcacc atcactgaag gcatcccgac gctggatatg ctgactgtga
    11221 aagtgaaact ggacgaagcc ggcgttcgta tgatcggccc gaactgcccg ggcgttatca
    11281 ctccgggtga atgcaaaatc ggtatccagc cgggtcacat tcacaaaccg ggtaaagtgg
    11341 gtatcgtttc ccgttccggt acactgacct atgaagcggt taaacagacc acggattacg
    11401 gtttcggtca gtcgacctgt gttggtatcg gcggtgaccc gatcccaggc tctaacttta
    11461 tcgacatcct cgaaatgttc gaaaaagatc cgcagaccga agcgatcgtg atgatcggtg
    11521 agatcggcgg tagcgctgaa gaagaagcgg cagcgtacat caaagagcac gttaccaagc
    11581 cagttgtagg ttacatcgct ggtgtgactg cgccgaaagg caaacgtatg ggccacgcgg
    11641 gtgcgatcat tgctggtggg aaagggactg cggatgagaa attcgctgct ctggaagccg
    11701 caggcgtgaa aaccgttcgc agcctggcgg atatcggtga agcactgaaa actgttctga
    11761 aataaatatc tgtaataaga aatagccctc gccgcttccc tctacaggaa tggcgaaggg
    11821 ctgtcggttt cgacatggtt ggccatcgta tgatggcctt ttttgtgctt atcgcgatga
    11881 ttttcgctgc gctatcaggg taaatttata gtcatcggta ttaaaagcgt tgcggctata
    11941 ttcaaacacc cgaccatcaa ctaaatatcc acgcgatact ttttcaagaa tcggctttgt
    12001 ctggctgata ttaagcagac ggctcatctc ttcggttggc atcagaggaa tgatttcctg
    12061 ttcgctacga tcgataacca tcttcttcac ttcttcaata aagtgatatt tcgaattttc
    12121 catgacctgc caggtgagat ccgggaacaa cgcaagcggc atccaggttt cttccagcgc
    12181 cattggcttt tgcttgcgat agcgcacgcg cttcacatgc cacacacgat cctgcgcggt
    12241 gatttgtagc tgttgctgaa gaaaatcgtc agccggaatc acttcaaata tcaggatttc
    12301 actgtgtgta tcgacgtgac ggtccgacag tttttcatca aaactggtta actgaaaaat
    12361 atcgtaattg acccgctctt ctttgacgta agtcccgctg ccctgaatgc tttcgaggat
    12421 ctgctgctcg actagctggc gcaaagcctg acgcaccgta acccggctga cgccaaactc
    12481 tgtttgtagc gctgattcag tgggtaacgc atcgccaggt ttaagctcgc cacgcgcaat
    12541 ttgttcacga atgcgatcgg caatctgccg gtataagggc ttgtgtccca tttttagtat
    12601 ctcattaata tgaatttagc cattatgccc gataaattca tcctgtaaat aatacaaata
    12661 caatacaaat aatttcaatc aagtgaaatt gatcacatga tggtattgtt ttatcgggca
    12721 cactggcgcg actataaaaa cgatcaagtg aggatcatga tgaatctgac gactctgacc
    12781 caccgcgatg cgttgtgtct gaatgcgcgc tttaccagcc gtgaagaggc catccacgcg
    12841 ttgactcaac gtcttgctgc tctggggaaa atttccagta ctgagcaatt tctggaagaa
    12901 gtgtatcgcc gtgaaagcct tggcccgacc gccttaggtg aagggttggc tgtgccgcat
    12961 ggcaaaactg ctgcggtaaa agaagcggcg tttgcggtcg ccacactcag cgagccgctt
    13021 cagtgggaag gcgttgatgg cccggaagca gttgatttag tggtgctgct ggcgattccc
    13081 cccaatgaag cgggtactac gcatatgcaa ctgctgacag cgctgaccac gcgccttgcg
    13141 gatgatgaga ttcgggcgcg tatacagtcg gcgacgacgc ctgatgagtt gctctcggcg
    13201 ctggatgaca agggaggcac gcaaccttct gcctcttttt ccaacgcgcc aactatcgtc
    13261 tgcgtaacgg cctgtccggc gggtattgct cacacctata tggctgcgga atatctggaa
    13321 aaagccggac gcaaactcgg cgtaaatgtt tacgttgaaa aacaaggcgc taacggcatt
    13381 gaagggcgtt taacggcgga tcaactcaat agtgcaaccg cctgtatttt tgcggctgaa
    13441 gtcgccatca aggagagtga gcgttttaat ggcattcccg cgctttcagt gcctgttgcc
    13501 gagccgattc gccatgcaga agcgttgatc caacaagcgc ttaccctcaa gcgtagcgat
    13561 gagacgcgta ccgtacagca agatacgcaa ccggtgaaaa gtgtcaaaac ggagctgaaa
    13621 caggcactgt tgagcggaat ctcttttgcc gtaccgttga ttgtcgcggg gggcacggtg
    13681 ctggcggtcg cggtattact gtcgcaaatc ttcgggctac aagatctgtt taatgaagaa
    13741 aactcctggc tgtggatgta ccgcaagctg ggcggcgggc tgctcggaat tttgatggta
    13801 ccggtgctcg cggcctatac cacctattct ctggcagata aaccggcgtt agcgccaggc
    13861 tttgcggctg gacttgccgc caacatgatc ggctccgggt ttctcggcgc ggtcgttggc
    13921 ggattgatag ccggttactt gatgcgctgg gtgaaaaatc acttgcgtct tagcagtaaa
    13981 ttcaatggat tcctgacttt ttatctctac ccggtgctcg gtacgttggg agcgggcagt
    14041 ctgatgctgt ttgtggtggg agaacctgtc gcatggatca ataactcgct taccgcctgg
    14101 ctgaacggtc tgtcaggaag taacgcgctg ttgctgggtg ccattctcgg ttttatgtgt
    14161 tcctttgacc ttggagggcc agtgaacaaa gccgcttatg cattctgcct gggcgcaatg
    14221 gcgaacggcg tttacggccc gtatgccatt ttcgcctccg tcaaaatggt ttcggcattt
    14281 accgtaaccg cttccacgat gctcgcaccg cgcctgttta aagagtttga aattgagacc
    14341 gggaaatcca cctggctgtt agggctggca ggtattaccg aaggggcgat cccgatggcg
    14401 attgaagatc cgctgcgggt tattggttcg tttgtgctgg gctctatggt aacgggcgct
    14461 attgtcggtg cgatgaatat cggcctttcg acacccggtg ccggcatttt ctcgctcttt
    14521 ttacttcatg ataatggcgc gggcggtgtt atggcggcaa ttggctggtt tggtgcggca
    14581 ttggtggggg ctgcaatctc gactgcaatt ctcctgatct ggcggcgtca cgcggttaag
    14641 catggcaact atctgactga tggcgtaatg ccataaacaa aaaggaaacg acgatgaaag
    14701 cggtatctcg cgttcacatc accccgcata tgcattggga tcgggagtgg tatttcacca
    14761 ccgaagagtc acgcattctg ctggtcaata atatggaaga gatcctgtgc cgactggaac
    14821 aggacaacga atacaaatat tacgtactcg acgggcaaac ggcgatcctc gaagattatt
    14881 tcgcggtgaa accggaaaac aaagaccgcg tgaagaaaca ggtagaagcc ggtaagttga
    14941 ttatcggccc ctggtatacc cagaccgata ccacgattgt ttctgcggaa tccatcgtcc
    15001 gtaatctgat gtacggaatg cgtgactgcc tcgcgtttgg cgagccgatg aaaataggtt
    15061 atttaccgga ttcctttggc atgtccgggc aactgccgca tatttacaat ggatttggca
    15121 ttacccgcac tatgttctgg cgcggatgtt cggagcgcca cggtactgat aaaaccgagt
    15181 ttttgtggca aagcagtgac ggtagcgaag tgacggcgca ggtgctgccg ctgggctacg
    15241 ccatcggtaa gtacttacct gccgacgaaa acggattacg taaacgcctc gacagttatt
    15301 ttgacgtgct ggaaaaagcg tctgtaacca aagagatttt gctgccgaat ggtcatgacc
    15361 agatgccatt gcagcaaaat atcttcgaag tgatggataa gctacgtgag atctaccctc
    15421 aacgtaagtt tgtgatgagc cgctttgaag aggtatttga gaagatcgaa gcgcagcgtg
    15481 aaagtctggc caccctgaaa ggtgaattta tcgatggcaa atatatgcgc gtacatcgca
    15541 ccatcggttc cacgcgcatg gatatcaaaa ttgcccacgc acgtattgaa aataagattg
    15601 ttaatctgct ggaaccgctg gcaacgctgg cctggacgtt gggttttgaa taccaccacg
    15661 gcttgctgga gaaaatgtgg aaagagatct taaaaaatca tgcccacgac agtatcggtt
    15721 gttgttgcag tgacaaagtt catcgcgaaa ttgtcgcccg cttcgaactg gctgaagaca
    15781 tggcggataa tctgctgcgt ttctacatgc gcaaaattgc cgataacatg ccacagagtg
    15841 atgccgacaa actcgtgctg tttaacctga tgccctggcc gcgggaagaa gttattaaca
    15901 ccactgtgcg gctgcgcgcc agccagttta atctacaaga cgatcgcggt cagcctgtac
    15961 cgtattttat tcgccatgcc cgtgagatcg atcctggcct aatcgatcgg caaattgttc
    16021 attatggcaa ttatgatccc tttatggagt ttgatattca gatcaaccag attgtccctt
    16081 ctatgggcta tcgcacgctt tatattgaag cgaatcagcc tggcaacgtg attgcggcaa
    16141 aaagtgacgc tgaagggatt ctggaaaatg ctttctggca gattgcgctc aatgaggatg
    16201 gttctctgca actggtagat aaagacagcg gtgtgcgcta tgaccgggta ttgcaaattg
    16261 aagaaagctc tgatgatggt gatgaatatg actattcacc cgcaaaagaa gagtgggtaa
    16321 ttaccgcagc gaacgcgaaa ccgcaatgcg atattattca tgaagcctgg cagagcaggg
    16381 ctgttatccg ctatgacatg gcagtgccgc tcaatttgtc agaacgcagc gcccggcaat
    16441 ccactggcag agtagggggg gtgttggttg tcactcttag tcataacagc aggcgtattg
    16501 atgtggatat caaccttgat aaccaggctg acgatcatcg ccttcgtgtc ctggtcccta
    16561 caccttttaa cactgacagt gttctggcag atacgcagtt tggttcgcta acgcgccccg
    16621 tgaacgacag tgcaatgaac aactggcagc aagaaggctg gaaagaagcg ccggttccgg
    16681 tatggaatat gctcaactat gttgccttac aggaagggcg taacggcatg gctgtcttta
    16741 gcgaagggtt acgtgaattt gaagtcatcg gtgaagagaa gaaaaccttt gccattacgt
    16801 tgctgcgtgg cgtgggctta ctgggcaaag aagatctgct tttaaggcct gggcggcctt
    16861 cgggaattaa aatgccagtc ccggactcac aactacgtgg tctgctttct tgtcgcctaa
    16921 gtttattgag ttataccggt acgccaaccg ccgctggtgt agctcagcag gcgcgagcat
    16981 ggctgactcc agtacagtgt tacaacaaaa tcccatggga tgcgatgaag ctcaacaaag
    17041 ccggattcaa cgtgccggaa agttatagtt tgttgaaaat gcccccagtg ggatgcctga
    17101 taagcgcact taagaaagct gaagaccgac aagaagtgat tttacggctg tttaatccgg
    17161 ctgaatcagc aacctgtgat gcgactgttg ctttcagtcg cgaggtgatt tcttgctcag
    17221 aaacgatgat ggatgaacac attaccaccg aggaaaatca aggttcaaat ctatcggggc
    17281 cttttttacc cggccagtca cggacgttca gttaccggct tgcctgaata gcaatcaaac
    17341 cgaagccaca tatgcgcggc cagattgttg acaaagggcg ctttgttcat gccggatacg
    17401 gcatgaacgc tttattcggt ctacaaaagc aggcaaattc aatatattgc agagatgatg
    17461 tagacactgg caagcgtagc gcatcaggta attttgcgtt tatcttcact ctcaagccac
    17521 gtatatgtgg ctttattttt aacaaaataa taaccctggg tgagttaatt ataatataat
    17581 tacaagttaa ttaaatgtta atatcggcgg ggtggattta tgcctttatt agtaatcctg
    17641 aaactctgcg tcgtattagc cagtgaccaa aaaaagaatt aaggtcaacc gtgctgtttt
    17701 tgcttcgtct ctttttatct ttaaattgcc aaccgaaact aatttcagcc ttataactca
    17761 cacattttaa acataaatgt cactaaagtt accttattga aacatgatta acataatttg
    17821 taggaattga tatttatcaa tgtataagtc ttggaaattg gcatcaaaaa gagataaatt
    17881 gttctcgatc aaattggctg aaaggcggta atttagctat aaattgatca ccgtcgaaaa
    17941 atgcaaattt gcttcaacaa aaacctgttt attgtaagga ttttgcagcg taatatatac
    18001 gtgggatcaa tttgagtttt tattaacatg tttgcaacct ttctttacgc cgtttttgtg
    18061 tgcattcaca tggtatgatg aaagtgttca aacaaatttc tattggggca tgcgtgtgac
    18121 cctttctaac ggggttcact ctcggagtct tcatgcgatg agcaaggagt catgatgtta
    18181 gatatagtcg aactgtcgcg cttacagttt gccttgaccg cgatgtacca cttccttttt
    18241 gtgccactga cgctcggtat ggcgttcctg ctggccatta tggaaacggt ctacgtcctc
    18301 tccggcaaac agatttataa agatatgacc aagttctggg gcaagttgtt tggtatcaac
    18361 ttcgctctgg gtgtggctac cggtctgacc atggagttcc agttcgggac taactggtct
    18421 tactattccc actatgtagg ggatatcttc ggtgcgccgc tggcaatcga aggtctgatg
    18481 gccttcttcc tcgaatccac ctttgtaggt ctgttcttct tcggttggga tcgtctgggt
    18541 aaagttcagc atatgtgtgt cacctggctg gtggcgctcg gttcaaacct gtccgcactg
    18601 tggattctgg ttgcgaacgg ctggatgcaa aacccaatcg cgtccgattt caactttgaa
    18661 actatgcgta tggagatggt gagcttctcc gagctggtgc ttaacccggt tgctcaggtg
    18721 aaattcgttc acactgtagc gtctggttat gtgactggcg cgatgttcat cctcggtatc
    18781 agcgcatggt atatgctgaa aggtcgtgac ttcgccttcg ctaaacgctc ctttgctatc
    18841 gctgccagct tcggtatggc tgctgttctg tctgttattg ttctgggtga tgaatccggc
    18901 tacgaaatgg gcgacgtgca gaaaaccaaa ctggctgcta ttgaagccga gtgggaaacg
    18961 caacctgcgc ctgctgcctt tactctgttc ggcattcctg atcaggaaga ggagacgaac
    19021 aaatttgcga ttcagatccc ttacgcactg ggcatcattg caacgcgttc cgtggatacc
    19081 ccggttatcg gcctgaaaga gctgatggtg cagcatgaag aacgcattcg taacgggatg
    19141 aaggcgtact ctctgctcga acaactgcgt tctggttcta ccgaccaggc ggttcgtgac
    19201 cagttcaata gcatgaagaa agacctcggt tacggtctgc tgctgaaacg ctatacgcca
    19261 aacgtggctg atgcgactga agcgcagatt caacaggcaa ccaaagactc catcccgcgt
    19321 gtagcgccgc tgtactttgc gttccgtatc atggtggcgt gtggcttcct gcttctggca
    19381 atcatcgcgc tctctttctg gagtgtcatc cgcaaccgca ttggcgagaa aaaatggctt
    19441 ctgcgcgccg cgctgtacgg tattccgctg ccgtggattg ctgtagaagc gggctggttc
    19501 gtggctgaat atggccgcca accgtgggct atcggtgaag tgctgccgac agctgtggcg
    19561 aactcgtcac tgaccgcagg cgatctcatc ttctcaatgg tgctgatttg cggcctgtat
    19621 accctgttcc tggtggcaga attgttctta atgttcaagt ttgcacgcct cggcccaagc
    19681 agcctgaaaa ccggtcgcta tcactttgag cagtcttcca cgactactca gccggcacgc
    19741 taagacagga gtcgtcaaat gatcgattat gaagtattgc gttttatctg gtggctgctg
    19801 gttggcgttc tgctgattgg ttttgcagtc actgacggtt tcgacatggg ggtgggcatg
    19861 ctcacccgtt tcctcggtcg taacgacacc gagcgtcgaa ttatgattaa ctccattgca
    19921 ccacactggg acggtaacca ggtttggctg atcaccgcgg gcggcgcact ctttgctgcc
    19981 tggccgatgg tctatgccgc tgcgttctcc ggcttctatg tggcgatgat cctcgtgctg
    20041 gcgtctttgt tcttccgtcc ggtcggtttt gactaccgct ccaagattga agaaacccgc
    20101 tggcgtaaca tgtgggactg gggcatcttc attggtagtt tcgttccgcc gctggtaatt
    20161 ggtgtagcgt tcggtaacct gttgcagggc gtaccgttca acgttgatga atatctgcgt
    20221 ctgtactaca ccggtaactt cttccagttg cttaacccgt tcggcctgct ggcaggcgtg
    20281 gtgagcgtag ggatgatcat tactcagggc gcaacctatc tgcaaatgcg taccgtgggc
    20341 gaactgcacc tgcgtacccg tgcaacggct caggtggctg cgctggtgac actggtctgt
    20401 ttcgcactgg ctggcgtatg ggtgatgtac ggtatcgatg gttatgtcgt gaaatcgaca
    20461 atggaccatt acgcagcctc taacccactg aataaagaag tggttcgtga agctggcgca
    20521 tggctggtta acttcaacaa cacgccaatt ctgtgggcta ttccggcact gggtgtggtt
    20581 cttccgctgc tgaccatcct gactgcacgt atggataaag ccgcgtgggc gtttgtgttc
    20641 tcctccctga cgctggcctg catcatcctg acagccggta tcgcaatgtt cccgtttgtg
    20701 atgccgtcca gcaccatgat gaacgcaagt ctgacaatgt gggatgcaac ttccagccag
    20761 ctgacgctta acgtcatgac ctgggttgcg gtggttctgg taccgatcat tctgctctac
    20821 accgcttggt gttactggaa aatgttcggt cgtatcacca aagaagatat tgaacgtaac
    20881 acccactctc tgtactaagt aaggagctaa aaatgtggta tttcgcatgg attctgggaa
    20941 cgcttcttgc ctgttcgttt ggggtaatca ccgcgctggc gcttgaacac gtcgaatcag
    21001 gcaaagccgg tcaagaagac atctgatgag taagattatc gcgaccttgt atgcggtaat
    21061 ggacaagcgc cccctgcggg cgctttcctt cgtgatggcg cttctgttag caggatgtat
    21121 gttttgggac ccatctcgtt ttgccgcgaa gaccagtgaa ctggaaatct ggcatggttt
    21181 attgctgatg tgggccgtct gtgctggtgt gattcacggc gtgggctttc gtccgcagaa
    21241 ggttctttgg caagggattt tttgcccatt gcttgccgat attgttctca ttgtcgggct
    21301 gattttcttc ttcttttaaa tcagaatttt ctttaaaaaa ttatgggccg ctccaggccc
    21361 ataaattttt acgctccctt aacttgccct cattcccaaa cctcaatcgc gcgcgtatag
    21421 tagcagcgtt taaaagctct aacttttgtt gcattaccgg gatgtaaagt gaatacaacg
    21481 ctgtttcgat ggccggttcg cgtctactat gaagataccg atgccggtgg tgtggtgtac
    21541 cacgccagtt acgtcgcttt ttatgaaaga gcacgcacag agatgctgcg tcatcatcac
    21601 ttcagtcagc aggcgctgat ggctgaacgc gttgcctttg tggtacgtaa aatgacggtg
    21661 gaatattacg cacctgcgcg gctcgacgat atgctcgaaa tacagactga aataacatca
    21721 atgcgtggca cctctttggt tttcacgcaa cgtattgtca acgccgagaa tactttgctg
    21781 aatgaagcag aggttctggt tgtttgcgtt gacccactca aaatgaagcc ttgtgcgctt
    21841 cccaagtcta ttgtcgcgga gtttaagcag tgactgacat gaatatcctt gatttgttcc
    21901 tgaaggctag ccttctggtt aaacttatca tgttgatttt gattggtttt tcaatcgcat
    21961 cctgggccat tattatccag cggacccgta ttcttaacgc agcggcgcgc gaagccgaag
    22021 cgtttgaaga taaattctgg tctggaatcg aactctctcg cctctatcaa gagagtcagg
    22081 ggaaacggga taatctgact ggttcggaac aaatatttta cagcgggttc aaagagtttg
    22141 tgcgcctgca tcgtgccaat agccatgcgc cggaagccgt agtggaaggg gcgtcgcgtg
    22201 ctatgcgtat ctccatgaac cgtgaacttg aaaatctgga aacgcacatt ccgttcctcg
    22261 gtacggttgg ctccatcagc ccgtatattg gtctgtttgg tacggtctgg gggatcatgc
    22321 acgcctttat cgccctcggg gcggtaaaac aagcaacact gcaaatggtt gcgcccggta
    22381 tcgcagaagc gttgattgcg actgcaattg gtctgttcgc cgcaattccg gcggtaatgg
    22441 cttataaccg cctcaaccag cgcgtaaaca aactggaact gaattacgac aactttatgg
    22501 aagagtttac cgcgattctg caccgccagg cgtttaccgt tagcgagagc aacaaggggt
    22561 aagccatggc cagagcgcgt ggacgaggtc gtcgcgatct caagtccgaa atcaacattg
    22621 taccgttgct ggacgtactg ctggtgctgt tgctgatctt tatggcgaca gcgcccatca
    22681 tcacccagag cgtggaggtc gatctgccag acgctactga atcacaggcg gtgagcagta
    22741 acgataatcc gccagtgatt gttgaagtgt ctggtattgg tcagtacacc gtggtggttg
    22801 agaaagatcg cctggagcgt ttaccaccag agcaggtggt ggcggaagtg tccagccgtt
    22861 tcaaggccaa cccgaaaacg gtctttctga tcggtggcgc aaaagatgtg ccttacgatg
    22921 aaataattaa agcactgaac ttgttacata gtgcgggtgt gaaatcggtt ggtttaatga
    22981 cgcagcctat ctaaacatct gcgtttccct tgcttgaaag agagcgggta acaggcgaac
    23041 agtttttgga aaccgagagt gtcaaaggca accgaacaaa acgacaagct caagcgggcg
    23101 ataattattt cagcagtgct gcatgtcatc ttatttgcgg cgctgctctg gagttcgttc
    23161 gatgagaata tagaagcttc agccggaggc ggcggtggtt cgtccatcga cgctgtcatg
    23221 gttgattcag gtgcggtagt tgagcagtac aaacgcatgc aaagccagga atcaagcgcg
    23281 aagcgttctg atgagcagcg caagataaag gaacagcagg ctgctgaaga actgcgtgag
    23341 aaacaagcgg ctgaacagga acgcctgaag caacttgaga aagagcggtt agctgctcag
    23401 gaacagaaaa agcaggctga agaagccgca aaacaggccg agttaaagca gaagcaagcg
    23461 gaagaggcgg cagcgaaagc ggcggcagat gctaaagcga aggccgaagc ggatgctaaa
    23521 gctgcggaag aagcagcgaa gaaagcggcg gcagacgcga agaaaaaagc agaagcagaa
    23581 gccgccaaag ccgcagccga agcgcagaaa aaagccgagg cagccgctgc ggcactgaag
    23641 aagaaagcgg aagcggcaga agctgctgca gctgaagcaa gaaagaaagc ggcagcagag
    23701 aaagctgcag ccgacaaaaa agcagcagaa aaagcggctg ctgataaggc agcagctgat
    23761 aagaaagcag cggcagaaaa agccgccgca gacaaaaaag cggcagcggc aaaagcagcg
    23821 gcagaaaaag ccgccgcaga caaaaaagcg gcagcggcaa aagcagcagc tgaaaaagcc
    23881 gctgcagcaa aagctgccgc agaggcagat gatattttcg gtgagctaag ctctggtaag
    23941 aatgcaccga aaacgggggg aggggcgaaa gggaacaatg cttcgtctgc cgggagtggt
    24001 aatactaaaa acaatggcgc atcaggggcc gatatcaata actatgccgg gcagattaaa
    24061 tctgctatcg aaagtaagtt ctatgacgca tcgtcctatg caggcaaaac ctgtacgctg
    24121 cgcataaaac tggcacccga tggtatgtta ctggatatca aacctgaagg tggcgatccc
    24181 gcactttgtc aggctgcgtt ggcagcagct aaacttgcga agatcccgaa accaccaagc
    24241 caggcagtat atgaagtgtt caaaaacgcg ccattggact tcaaaccgta atcgcgatgt
    24301 tgactgttcc gacggtcaac atcaggcacc ggttgccacg gggttctggt agttttgtgt
    24361 attttagttt gttaacattc tgctaaatta tcgtgggcca tcggtccaga taagggagat
    24421 atgatgaagc aggcattacg agtagcattt ggttttctca tactgtgggc atcagttctg
    24481 catgctgaag tccgcattgt gatcgacagc ggtgtagatt ccggtcgtcc tattggtgtt
    24541 gttcctttcc agtgggcggg ccctggtgcg gcacctgaag atattggcgg catcgttgct
    24601 gccgacttgc gtaacagcgg taaatttaat ccgttagatc gcgctcgtct gccacagcag
    24661 ccgggtagtg cgcaggaagt acaaccagct gcatggtccg cgctgggtat tgacgctgta
    24721 gttgtcggtc aggtcactcc gaatccggat ggttcttaca atgttgctta tcaacttgtt
    24781 gacactggcg gcgcaccggg tactgtactt gctcagaact cgtacaaagt gaacaagcag
    24841 tggctgcgct atgctggtca taccgccagt gatgaagtgt ttgaaaaact gaccggcatt
    24901 aaaggtgcgt tccgtacccg tattgcctac gttgttcaga ccaacggcgg tcagttcccg
    24961 tatgaactgc gcgtatctga ctatgacggt tacaaccagt ttgtcgttca ccgttcacca
    25021 cagccgctga tgtctccggc gtggtcacca gacggttcta aactggctta tgtgaccttc
    25081 gaaagcggtc gttccgcgct agttatccaa acgctggcaa acggcgctgt acgtcaggtg
    25141 gcttcattcc cgcgtcacaa cggtgcgcct gcattctcgc cagacggcag caaactggca
    25201 ttcgccttgt cgaaaaccgg tagcctgaac ctgtacgtaa tggatttggc ttctggtcag
    25261 atccgccagg tgactgatgg tcgcagtaac aataccgaac cgacctggtt cccggacagc
    25321 cagaacctgg catttacttc tgaccaggcc ggtcgtccac aggtttataa agtgaatatc
    25381 aacggcggtg cgccacaacg tattacctgg gaaggttcgc agaaccagga tgcggatgtc
    25441 agcagcgacg gtaaatttat ggtaatggtc agctccaacg gtgggcagca gcacattgcc
    25501 aaacaagatc tggcaacggg aggcgtacaa gttctgtcgt ccacgttcct ggatgaaacg
    25561 ccaagtctgg cacctaacgg cactatggta atctacagct cttctcaggg gatgggatcc
    25621 gtgctgaatt tggtttctac agatgggcgt ttcaaagcgc gtcttccggc aactgatgga
    25681 caggtcaaat tccctgcctg gtcgccgtat ctgtgataat aattaattga atagtaaagg
    25741 aatcattgaa atgcaactga acaaagtgct gaaagggctg atgattgctc tgcctgttat
    25801 ggcaattgcg gcatgttctt ccaacaagaa cgccagcaat gacggcagcg aaggcatgct
    25861 gggtgccggc actggtatgg atgcgaacgg cggcaacggc aacatgtctt ccgaagagca
    25921 ggctcgtctg caaatgcaac agctgcagca gaacaacatc gtttacttcg atctggacaa
    25981 gtacgatatc cgttctgact tcgctcaaat gctggatgca catgcaaact tcctgcgtag
    26041 caacccgtct tacaaagtca ccgtagaagg tcacgcggac gaacgtggta ctccggaata
    26101 caacatctcc ctgggtgaac gtcgtgcgaa cgccgttaag atgtacctgc agggtaaagg
    26161 cgtttctgca gaccagatct ccatcgtttc ttacggtaaa gaaaaacctg cagtactggg
    26221 tcatgacgaa gcggcatact ccaaaaaccg tcgtgcggta ctggtttact aagagaattg
    26281 catgagcagt aacttcagac atcaactatt gagtctgtcg ttactggttg gtatagcggc
    26341 cccctgggcc gcttttgctc aggcaccaat cagtagtgtc ggctcaggct cggtcgaaga
    26401 ccgcgtcact caacttgagc gtatttctaa tgctcacagc cagcttttaa cccaactcca
    26461 gcaacaactt tctgataatc aatccgatat tgattccctg cgtggtcaga ttcaggaaaa
    26521 tcagtatcaa ctgaatcagg tcgtggagcg gcaaaagcag atcctgttgc agatcgacag
    26581 cctcagcagc ggtggtgcag cggcgcaatc aaccagcagc gatcaaagcg gtgcggcggc
    26641 atcaacgacg ccgacagctg atgctggtac tgcgaatgct ggcgcgccgg tgaaaagcgg
    26701 cgatgcaaac acggattaca atgcagctat tgcgctggtg caggataaat cccgccagga
    26761 tgacgcaatg gtggcatttc agaatttcat caaaaattac cctgattcaa cttacctgcc
    26821 aaacgccaat tattggctgg gtcagttaaa ctacaacaag ggtaaaaaag atgatgcggc
    26881 gtactatttt gcttcggtag tgaaaaacta tccgaagtca ccaaaggctg ctgatgcgat
    26941 gtttaaagtc ggcgtcatca tgcaggacaa aggtgacacc gcaaaagcga aagccgtata
    27001 ccagcaggtt atcagtaaat accctggtac tgatggcgct aaacaggcac aaaaacgtct
    27061 gaacgcgatg taatgcataa cacacgacca gaagtcgcat tatttctggt cgtgtcgtgc
    27121 gaatcataag cagttgagtg atctgcatcg aaatttttgt tgcgctcaag tctgaaatca
    27181 gtaatatatg ccgccgttgc cacgggatat caaacaaacc gaaagcaacg aaaaagtggg
    27241 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
    27301 gcacgaccca ccagttttag catcgaagac agatgttaag cgtgtaggat aacgttgcgt
    27361 cagcaacggc ccgtagggcg agcgaagcga gtcatcctgc acgacccacc actaattacg
    27421 gtgggttcgg tggaagtagt ttgtagtatc cagcgcagta tcgggtgatt agctcagctg
    27481 ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact
    27541 cgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa
    27601 tcctgcacga cccaccaatc ttaaagattg gccccgagta gaaatctttc aggtaacacc
    27661 cgtatgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
    27721 cgaatcctgc acgacccacc atcctgaatg attaaagcag cataatcccg caaggggtcg
    27781 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
    27841 cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctga gatattcaaa
    27901 gattcgaacc tgcagcaggt tcgagttgag cgcagcgaaa caacggagcc gctcgcggcg
    27961 acggcccgaa gggcgagcga agcgagtcat cctgcacgac ccaccaatgt aaaaaagcgc
    28021 cctaaaggcg cttttttgct attcaggcat cctcaatttc actttgtaaa cctgatgaca
    28081 tcgtcagagc ttactgtgca agcaactcta cgtcggtgga attaggcgta attcgagtga
    28141 cttttcccca ccattcgact atcttgttta gcatataaaa caaattacgc cgatagtagc
    28201 gaatattacg ctaatgtcgg ttttaacgtt aagcctgtaa aacgagatgg taagatgagc
    28261 gtgatgtttg atccagacac ggcgatttat cctttccccc cgaagccgac gccgttaagc
    28321 attgatgaaa aagcgtatta ccgcgagaag ataaaacgtc tgctaaaaga acgtaatgcg
    28381 gtgatggttg cccactacta taccgacccc gaaattcaac aactggcaga agaaaccggt
    28441 ggctgtattt ctgattctct ggaaatggcg cgcttcggtg caaagcatcc cgcttctacg
    28501 ttgttagtcg ctggggtgag atttatggga gaaaccgcca aaattctcag tccggaaaaa
    28561 acaattctga tgctgacact tcaggctgaa tgttcactgg atctcggctg ccctgttgaa
    28621 gaatttaacg cattttgcga tgcccatccc gatcatactg tcgtcgtcta cgccaacact
    28681 tctgctgcgg taaaagcgcg cgcagattgg gtggtaactt caagcattgc cgtcgaactt
    28741 attgatcatc tggatagttt tggtgaaaaa atcatctggg cacccgacaa acatctgggg
    28801 cgttacgtgc aaaaacagac gggcgcagac attttatgct ggcagggtgc ctgtattgtg
    28861 catgatgaat ttaagactca ggcgttaacc cgcttgcaag aagaataccc ggatgctgcc
    28921 atactggtgc atccagaatc accacaagct attgtcgata tggcggatgc ggtcggttcc
    28981 accagtcaac tgatcgctgc tgcgaaaaca ttgccacatc agcggcttat cgtggcaacc
    29041 gatcggggca ttttctacaa aatgcagcag gcggtgccag ataaagagtt actggaagca
    29101 ccaaccgcag gtgagggtgc aacctgccgc a
//
LOCUS       DAZOXR010000037        28815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZOXR010000037 DAZOXR010000000
VERSION     DAZOXR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.037"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(406..612)
                     /locus_tag="ACHUNN_001382"
     CDS             complement(406..612)
                     /locus_tag="ACHUNN_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0575799.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            925..1014
                     /gene="kdpF"
                     /locus_tag="ACHUNN_001383"
     CDS             925..1014
                     /gene="kdpF"
                     /locus_tag="ACHUNN_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0575800.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1014..2687
                     /gene="kdpA"
                     /locus_tag="ACHUNN_001384"
     CDS             1014..2687
                     /gene="kdpA"
                     /locus_tag="ACHUNN_001384"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0575801.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2710..4758
                     /gene="kdpB"
                     /locus_tag="ACHUNN_001385"
     CDS             2710..4758
                     /gene="kdpB"
                     /locus_tag="ACHUNN_001385"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR0575802.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4767..5339
                     /gene="kdpC"
                     /locus_tag="ACHUNN_001386"
     CDS             4767..5339
                     /gene="kdpC"
                     /locus_tag="ACHUNN_001386"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0575803.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5332..8016
                     /gene="kdpD"
                     /locus_tag="ACHUNN_001387"
     CDS             5332..8016
                     /gene="kdpD"
                     /locus_tag="ACHUNN_001387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR0575804.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8013..8690
                     /gene="kdpE"
                     /locus_tag="ACHUNN_001388"
     CDS             8013..8690
                     /gene="kdpE"
                     /locus_tag="ACHUNN_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR0575805.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8852..8986
                     /locus_tag="ACHUNN_001389"
     CDS             8852..8986
                     /locus_tag="ACHUNN_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0575806.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            8965..9069
                     /gene="speFL"
                     /locus_tag="ACHUNN_001390"
     CDS             8965..9069
                     /gene="speFL"
                     /locus_tag="ACHUNN_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0575807.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9380..11578
                     /gene="speF"
                     /locus_tag="ACHUNN_001391"
     CDS             9380..11578
                     /gene="speF"
                     /locus_tag="ACHUNN_001391"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR0575808.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11575..12894
                     /gene="potE"
                     /locus_tag="ACHUNN_001392"
     CDS             11575..12894
                     /gene="potE"
                     /locus_tag="ACHUNN_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0575809.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12950..14590)
                     /gene="pgm"
                     /locus_tag="ACHUNN_001393"
     CDS             complement(12950..14590)
                     /gene="pgm"
                     /locus_tag="ACHUNN_001393"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0575810.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14616..15161)
                     /gene="seqA"
                     /locus_tag="ACHUNN_001394"
     CDS             complement(14616..15161)
                     /gene="seqA"
                     /locus_tag="ACHUNN_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0575811.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15346..16110
                     /gene="ybfF"
                     /locus_tag="ACHUNN_001395"
     CDS             15346..16110
                     /gene="ybfF"
                     /locus_tag="ACHUNN_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0575812.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16250..16543
                     /gene="ybfE"
                     /locus_tag="ACHUNN_001396"
     CDS             16250..16543
                     /gene="ybfE"
                     /locus_tag="ACHUNN_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0575813.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16683..17213
                     /gene="fldA"
                     /locus_tag="ACHUNN_001397"
     CDS             16683..17213
                     /gene="fldA"
                     /locus_tag="ACHUNN_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0575814.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17423..17509
                     /gene="uof"
                     /locus_tag="ACHUNN_001398"
     CDS             17423..17509
                     /gene="uof"
                     /locus_tag="ACHUNN_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0575815.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17502..17948
                     /gene="fur"
                     /locus_tag="ACHUNN_001399"
     CDS             17502..17948
                     /gene="fur"
                     /locus_tag="ACHUNN_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0575816.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18033..18359)
                     /gene="chiQ"
                     /locus_tag="ACHUNN_001400"
     CDS             complement(18033..18359)
                     /gene="chiQ"
                     /locus_tag="ACHUNN_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0575817.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="ACHUNN_001401"
                     /pseudo
     CDS             complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="ACHUNN_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20393..22057)
                     /gene="glnS"
                     /locus_tag="ACHUNN_001402"
     CDS             complement(20393..22057)
                     /gene="glnS"
                     /locus_tag="ACHUNN_001402"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0575818.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22317..24263)
                     /gene="nagE"
                     /locus_tag="ACHUNN_001403"
     CDS             complement(22317..24263)
                     /gene="nagE"
                     /locus_tag="ACHUNN_001403"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0575819.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24596..25396
                     /gene="nagB"
                     /locus_tag="ACHUNN_001404"
     CDS             24596..25396
                     /gene="nagB"
                     /locus_tag="ACHUNN_001404"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0575820.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25456..26604
                     /gene="nagA"
                     /locus_tag="ACHUNN_001405"
     CDS             25456..26604
                     /gene="nagA"
                     /locus_tag="ACHUNN_001405"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0575821.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26613..27833
                     /gene="nagC"
                     /locus_tag="ACHUNN_001406"
                     /gene_synonym="nagR"
     CDS             26613..27833
                     /gene="nagC"
                     /locus_tag="ACHUNN_001406"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0575822.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27881..28633
                     /gene="nagD"
                     /locus_tag="ACHUNN_001407"
     CDS             27881..28633
                     /gene="nagD"
                     /locus_tag="ACHUNN_001407"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR0575823.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(28684..28815)
                     /locus_tag="ACHUNN_001408"
                     /pseudo
     CDS             complement(28684..28815)
                     /locus_tag="ACHUNN_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6751 a         7353 c         7561 g         7150 t
ORIGIN      
        1 gcaccgagca ggggattgac cggatggccg gacgtcactc cgccggggca caccgaacag
       61 gccacaccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatttctca tccttgtttt
      181 atcgggttat ttgtccgctg acaggcaaga ccctacagat ttataagaaa gccttaagtg
      241 agatatccgt cagatagaaa caattaggaa aatatttata tatatttcat gaatatatgt
      301 ttcgattggt ataacggtgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg
      361 ccagcgtttc cgctggcggt tttcaatgta ttcagaactc agcaatcagt aaaaatcacc
      421 ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      481 gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      541 aacgcccgct gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct
      601 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat
      661 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      721 cacttatttt tgcttattga tggtttattt acattcactc tgtaattaag ttacacaaaa
      781 gttaaattaa tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta
      841 caccccgccc gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca
      901 cttttaggcc actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg
      961 tttttattac tgggttattt ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg
     1021 cgcaagggtt cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtcctt
     1081 taggcagcgg gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg
     1141 aacgtatact ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc
     1201 tttgtgccat tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg
     1261 gtcagcacta tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc
     1321 tgaataccgc cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca
     1381 cgttgagcta tttcagccag atggcaggct taacggtaca aaactttctt tctgccgcca
     1441 gcgggattgc ggtgattttt gccttcatcc gtgcgtttac tcgccagagc atgagcacac
     1501 tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgctagtc cctgtggcgt
     1561 tgttgatcgc actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg
     1621 cagtgaatac cgttgaagga gcgcaacagc tgttacccat gggaccagta gcttctcagg
     1681 aagcgatcaa gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc
     1741 cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc
     1801 caacagcgct gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt
     1861 tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg cgtggttatg tgggcagaag
     1921 ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta
     1981 aagagagccg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt
     2041 cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc tctcggcggc atggtgccga
     2101 tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt cggttctggt ctttacggca
     2161 tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg
     2221 aatatctggg taaaaaaatc gacgtacgcg agatgaaact gactgcactg gcgattctgg
     2281 ttactccaac actggtactg atgggcgcgg cgttggcgat gatgaccgac gccggacgta
     2341 gcgccatgct taaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcgtccg
     2401 ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact
     2461 gtttactggc gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa
     2521 ttgccggttc gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc
     2581 acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct
     2641 ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagcga
     2701 gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg
     2761 ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt
     2821 atcgtctgga tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg
     2881 atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg
     2941 ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt
     3001 aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg
     3061 gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc
     3121 gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc
     3181 atcaccgggg aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc
     3241 ggcggcacgc gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag
     3301 acatttctgg atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac
     3361 gagattgccc tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc
     3421 acgctgtggc cgttttccgc gtggggcggt aatgccgtga gtgtaacggt actggtggcg
     3481 ctgctggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc
     3541 gggatgagtc ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg
     3601 gcaggtgacg ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt
     3661 caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa aaacgctggc tgacgccgca
     3721 caactggctt cgctggctga tgaaacgccg gaaggtcgca gtattgtgat cctcgccaag
     3781 caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt
     3841 actgcgcaaa gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct
     3901 gtcgatgcca ttcgccgcca tattgaggct aacggcggtc acttccctgc cgatgttgat
     3961 caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc tggtggtggt ggaaggttct
     4021 cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc
     4081 gcccagctgc gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact
     4141 gccgccgcga tagctgcgga agcgggtgtc gatgattttc tcgccgaagc aacaccggag
     4201 gccaagctgg cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc
     4261 gacggcacca acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc
     4321 ggcacccagg cggcgaaaga ggcgggcaat atggtggatc tcgactctaa cccgaccaag
     4381 ttgatcgagg tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc
     4441 ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca
     4501 acatatccgc agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt
     4561 ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa
     4621 ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac
     4681 ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc
     4741 gtttgcggtc tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc
     4801 tctttctgtt attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat
     4861 ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcgtggct
     4921 cggcattaat cgggcagaat tttaccggca acggctattt tcatggtcgc ccgtcagcaa
     4981 cggctgaaat gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc
     5041 ctgagctgga taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg
     5101 ccagcgcgag cattccggtt gaactggtga cggcatcggc aagcgggctg gacaataata
     5161 tcaccccgca agcggcggcc tggcagatcc cacgcatcgc gaaagcgcgt aatctcagcg
     5221 ttgaacagct cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg
     5281 gccagccggt cgtcaacatt gtcgaactca atctggcgct ggataagctt gatgaataac
     5341 gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg
     5401 aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca
     5461 gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac
     5521 gggcgaaaag ataccgccgc aatgctggaa gggctggctg ttctgccgcc aaaacgcctg
     5581 gcgtaccgtg ggcggcacat ctgcgagttt gacctcgatg ccgccctcgc ccgccgtccg
     5641 gcgctgatct taatggacga actggcgcac agtaatgcgc caggttctcg tcaccctaaa
     5701 cgctggcagg acatcgaaga gctgctggaa gcaggcattg atgttttcac taccgtcaac
     5761 gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg
     5821 gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg tggttctggt ggacttgccc
     5881 ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag
     5941 cgcgccattg aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg
     6001 cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctagcgaa
     6061 gagaaagtct ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggtagt
     6121 gagaagctgg tccgcgcggc ggcgcggctg gcgtcacggc tgggtagcgt ctggcacgcg
     6181 gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg tgcaattctc
     6241 agcgccttac gtctggcgca ggaactgggc gcggagacgg caacactttc tgatccagcg
     6301 gaagagaaag cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt
     6361 cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc
     6421 gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac
     6481 aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg
     6541 gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt
     6601 gatgccgcca acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat
     6661 ggacgctggc cttcggtggt tgccaccgtc attaatgtgg tgagtttcga tctctttttt
     6721 atcgccccac gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg
     6781 atgttaaccg tcgggctggt gatcgggaac cttactgctg gcgtgcgtta tcaggcgcgg
     6841 gtagcccgtt accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg
     6901 gtgggccgca gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt
     6961 catgcccgca gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat
     7021 ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg
     7081 cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt accagatttt gccgctaaaa
     7141 agcggcgaga aaacctacgg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg
     7201 atcccggaac agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcccttgag
     7261 cggctgacgc taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc
     7321 cgcaacgccc tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt
     7381 ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag
     7441 gccagcgaga ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat
     7501 atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa
     7561 gtagtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct
     7621 ctgccagaac cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt
     7681 aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc
     7741 cactttgagg gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca
     7801 ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg
     7861 ggtgtcgggc ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt
     7921 accgcgttca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc
     7981 gctcctgaac ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg
     8041 aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg
     8101 aggccgaaac tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta
     8161 ttctcgatct cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt
     8221 ggagcccggt gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg
     8281 cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg
     8341 cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8401 aattttccga tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8461 tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag
     8521 tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc
     8581 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc
     8641 ctcgccactt cattactgaa accggtattg gatatcggtt tatgctttga attgttattc
     8701 aataaattcg cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt
     8761 ggcaggttta atttcttaac gcaaatataa aacacgaaat tacaacattt aagtaacaaa
     8821 acagcatcta atatggcgat attcataatt aatgatttat tatctggggt atgatcgttt
     8881 tttgttgatc ttcttcacag attatagcca tttcatggat agaataactc taccttcaac
     8941 tgacacagca agaggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg
     9001 cggacaaccc atatcatgaa gtttgcccat cgtaatagtt ttgactttca tttctttaat
     9061 gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat
     9121 tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa
     9181 taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg
     9241 gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt
     9301 tgcttttttt ccggcgtgat cgatttctcc tttgagaaat tgaggacctg ctattacctg
     9361 aaataaagag atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga
     9421 ttgtttttcc actcatcgtg catgcgtgaa tattgacgaa agtaattata ttgacgttgc
     9481 cgccattatt ttatcagtca atgatgttga acgtggaaaa ctcgatgaaa tagacgctac
     9541 tggctatggc attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata
     9601 tctgccacgt atttctggtg tcttcgagca ttgcgaatca cggaaagagt tttatggacg
     9661 tcagttagaa accgctgcta gccattatga aacgcaactg cgcccgccct tcttccgcgc
     9721 actggtcgat tatgtcaatc agggcaatag cgcttttgac tgcccgggtc atcagggagg
     9781 tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg gagaaacgct
     9841 gttccgttcc gacttatgca acgcagacgt agcaatgggc gatctgctga ttcacgaagg
     9901 cgcgccatgc attgcacagc aacatgcggc aaaagtgttt aatgccgata aaacatactt
     9961 cgttctaaat ggcacttcat cttctaacaa agtggtttta aacgccctgc taacaccggg
    10021 tgatctggtg ctgtttgatc gcaataacca caaatctaac caccacggag cgttgctaca
    10081 ggctggtgca acaccggttt atctggaaac ggcacgtaac ccgtatggct ttatcggtgg
    10141 cattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc
    10201 gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga
    10261 cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat
    10321 tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc
    10381 gccgctgttg ctggatctta atgagaacga tccgggtatt ctggttacgc aatccgtaca
    10441 taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa
    10501 agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcacgcctc
    10561 caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg
    10621 tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaaact
    10681 gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc
    10741 ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc
    10801 aggggaacac tggcattctt ttgaaggcta tgcagagaat caatactttg tcgatccttg
    10861 caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg
    10921 tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg
    10981 cgatcttaac tccatcctct tcctgctgac tccggcagaa gatatggcca aacttcagca
    11041 acttgttgcc ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga
    11101 agtgctgcct tccatctaca aacagcatga agagcgctac gccggttata ccctgcgtca
    11161 gttgtgccag gaaatgcatg atttgtatgc ccgccacaac gtgaaacaac tgcaaaaaga
    11221 gatgttccgc aaagcgcact tcccacacgt cagcatgaat ccgcaagaag ccaactacgc
    11281 ctatttacgc ggtgaagtgg agctggttcg tctgccggat gccgaaggtc gtatcgctgc
    11341 cgaaggggcg cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg
    11401 tggtgctgtt ttgcgttact tcagcgctct ggaagaaggg atcaacctgc tgccaggttt
    11461 tgcaccggag ctgcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg
    11521 ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt
    11581 caggctaaat cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg
    11641 atgggctccg gtatcatcat gctgccgaca aagcttgccg aagttgggac aatttcaatt
    11701 atctcctggc tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc
    11761 ggtatgttca gccgtaaatc aggtggtatg ggcggttatg ccgagtatgc tttcggtaaa
    11821 tccggtaact ttatggcgaa ctatacctac ggcgtctcac tgctgattgc taacgtcgcg
    11881 attgctattt cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg
    11941 cagattggtc ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt
    12001 gcgcgcatta ccgggcaaat cagtagcatt accgtgtggg gggtcattat tccggtggta
    12061 ggcctgtgca ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat
    12121 ccgcatcatg caccgttctt cagtgcagta ggttcttcca tcgctatgac gctgtgggct
    12181 tttctcggtc tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat
    12241 gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc
    12301 aacgtgattg cagggattgt gcctaatatg gagctggcaa attcaacggc accatttggc
    12361 ctggccttcg cacagatgtt cacaccagaa gtgggtaaag tcattatggc gctgatggtg
    12421 atgtcctgct gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaagtct
    12481 tcatctgatg aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg
    12541 gtgcagggaa tgttgaccat tgtgattatc cagagcgggt tggcgctgat gacgattagc
    12601 ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatt
    12661 ccgtatattc tgtcaatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc
    12721 tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg
    12781 ctgtactcat ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg
    12841 acactgtatg gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc
    12901 aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat taaatgtgtt tacgcgtttt
    12961 tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac
    13021 cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga
    13081 accagccgtt gtcagtcatc actttcagac cgccgataga agcaccattg cccggtgccg
    13141 ccgtcaggcg cgcggtgatc gggtcgcctg ccagggtgct ggcgctcacc atttccggag
    13201 acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc
    13261 tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga
    13321 cagcggtgat ttccgccgcc agcagacaca tgatgatgcc gtctttgtcg gtggaccacg
    13381 gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc
    13441 tgccgtcgaa cagaccatca acaaaccatt tgaaacctac cgggacttct accagcttgc
    13501 ggcccaagtc attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag
    13561 caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt
    13621 agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt
    13681 cgttagcaaa cgccagatcg aacttatcac gcaatgccag caggcccgcc atcgcacact
    13741 cggaggagca gtccatacgg atcgcgccgt ctttatcgag gtgcataaag cggaaggttt
    13801 gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca attcgcttcc
    13861 agtattcgat accggaacca cccagcggat caacgcccag cgtcaggccc gttttctgaa
    13921 tcgccgccat atcaacgata tcggccagac cttccacaaa ctgctgcacc agatcctgct
    13981 ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc
    14041 catcggccat cagtgcgttg gcacgatctt ccaccacttt agtgacgttg gtatcagccg
    14101 ggccaccatt tggcggattg tacttgatac caccatcttc cggcgggtta tgggagggtg
    14161 taatcacgat accgtctgcc agcgggccac cttttttatt atgaaccagg atggcattgg
    14221 aaacggcagg cgtcggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg
    14281 ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac
    14341 ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga
    14401 tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag
    14461 taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg
    14521 ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt
    14581 ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca
    14641 atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg
    14701 ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc
    14761 tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg
    14821 tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac
    14881 agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcgtcaga aagcagaagt
    14941 tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca
    15001 ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat
    15061 ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca
    15121 atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca gtacagtgtc
    15181 ttagctgaat acaagtcgcc aagagtatat gaataaatcc cggcagcgga tagcgtcaag
    15241 cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt
    15301 caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc
    15361 cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg
    15421 tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc
    15481 atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg
    15541 gcgatggcgc aggatcttgt tgatactctg gatgcacagc agatcgacaa agcaacattt
    15601 atcggtcact ccatgggcgg taaagcggta atggcactta cggcactggc ccgcgatcgc
    15661 atcgataaac tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat
    15721 gagattttcg cggctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca
    15781 gcagcaataa tgcaccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct
    15841 tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt
    15901 gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat
    15961 tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg
    16021 gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta
    16081 cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt
    16141 cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc
    16201 catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga
    16261 acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa
    16321 aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg
    16381 cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc
    16441 gctgaacgag ctggcggagt cgcgcaatat gagccgtagc gaactgatcg aagagatgtt
    16501 aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta
    16561 gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt
    16621 gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac
    16681 tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa
    16741 aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca
    16801 gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc tggtattatg
    16861 gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg
    16921 gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg
    16981 acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaacgatc gttggtcact
    17041 ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggctgatgac gaccactttg
    17101 tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat
    17161 gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tctcaatgcc tgatgtgatg
    17221 cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca
    17281 aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt
    17341 tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc
    17401 atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc
    17461 ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg
    17521 ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt aaaaatccta gaagttcttc
    17581 aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg
    17641 gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta
    17701 tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc
    17761 accacgatca cctgatctgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca
    17821 tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc
    17881 tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgaacat gcgcacgaag
    17941 gcaaataagc cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatgcaagg
    18001 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc
    18061 caaactttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
    18121 tactgctgat aatccagtac gcggacactt tcctggtcgg caaaatgctg atgttgtttc
    18181 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt
    18241 gcggtgttga tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt
    18301 gattgcgcac aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata
    18361 gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
    18421 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt tgccgtaacc
    18481 accgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
    18541 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt atgcagactc
    18601 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgctagg tcgcaggttt
    18661 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
    18721 caggtcatac atcgcaccga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
    18781 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg
    18841 caggaagtat ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac
    18901 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    18961 gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt
    19021 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    19081 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    19141 cccaagggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    19201 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaacgcacc
    19261 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa
    19321 gctccagtgc ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca
    19381 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    19441 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    19501 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    19561 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    19621 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    19681 tataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    19741 cataaagccc gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc
    19801 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    19861 agcgaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca
    19921 gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga
    19981 gcaaccgcac atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt
    20041 gcagaagaat aaaagcgtga aattccagca agatgaaagc ggttacattt tatttaatta
    20101 tgttttgtta ttttaaattt attttatgga taaattgcga agcggaatta attcgcgaag
    20161 aaaagcagaa aaaacgccgc tgaacgcggc gttttggatt gtttgcatat ttcttgccgg
    20221 atgcggcgtg aacgccttaa ccggcctaca aaagcacgca aactcaatat attgcagaga
    20281 tcatgtaggc ctgataagcg tagtgcatca ggcaatttag cgtttgaaac tgtttgtctg
    20341 ttccttgccg gatgcgacgt aaacgcccca tccggcatag cgaaacttaa aattactcgc
    20401 ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg
    20461 tagaatggcg gctatcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg
    20521 caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt
    20581 taatcaccga caggaaatca tccgcagcac cagggttcgg cacgctgaac agacggtcgt
    20641 acagacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga
    20701 ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg
    20761 tggtgatatt gccttcggca tctttctcca cgcgttctgc cttaatgacg taagcattac
    20821 gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga
    20881 aatcggcgcg atcgatccaa atctcaccgc taaacggcac ctggcggcta cccatttccg
    20941 gtttgttcgg atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa
    21001 ccagtttcac cggatcgata accgccattg cgcgcggggc gttttcgttg agatcttcac
    21061 ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc
    21121 gtttgcagaa ctcacgaata gaagcagcag tgtaaccacg acgacgcaga ccggaaatgg
    21181 tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct
    21241 tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt
    21301 gaacaggaat ggtgatgttg tccagcaccc agtcgtacag gcgacggttg tcctggaact
    21361 caagcgtaca cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt
    21421 cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc
    21481 ggtacagcac cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca
    21541 ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaggttct
    21601 cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt
    21661 attcgcggat ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt
    21721 cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc
    21781 agtgaaaacc taaccactct acgtcgtttt tgatcgactc aacatactcg atatcttctt
    21841 ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc
    21901 cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg
    21961 ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac
    22021 ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac
    22081 aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagcgaaa
    22141 atcgaaaatc tcactgagat tatggggatg attgatgaaa aaaaaaagcg gcagagaaga
    22201 tctccaccgc ttttgccagt cccgcgtcat tgttggatgc gcgtcttatc caaccaggac
    22261 tgagcttttg taggcctgat aagacgcgtc aagcgtcgca tcaggcataa agcagattac
    22321 tttttgattt catacagcgg tgtttgaccc gccacaacat tgccctgagc tttaatgatc
    22381 aagccactga aatcgtcgat attgctgcaa accaccgggc taatcatcga acgggcgtta
    22441 gcgttcaggt aatccagatc catttccaga atcggttgcc ctgcgctcac ctgcgcgccc
    22501 tcttccacca gacgtttaaa gcctttacct tccagcgcta cggtgtcgat acccatgtgg
    22561 acgacaatct ccgcgccttt ttcggtttct aggcagaacg cgtggttggt gttgaagatt
    22621 ttcacgattg tccctgcggc tggtgatacg acgattttat ctgtcggttt caccgccaca
    22681 ccgtcaccca ccgctttgct ggcgaatgct tcgtcagaaa cctgatccag tgccacgaca
    22741 tcaccggtaa tcggcgatac cagctccgcg atagataccg cgtttggtac agcctgcggt
    22801 tttgctacag gagcggcagt tgccggagtt gcttcagctg acgcagctgc taccgggcca
    22861 cgggcaacga ctttcttcat cgcatcgcca atggattctg ctttcgcgcc aacaatcacc
    22921 tgaatggtct gtttgttcag ttttactaca ccagaagcac ccagacgttt acacatcgca
    22981 tcgttgacgc gagctgagtc agccacggta aggcgcagac gggtaataca ggcgtcaatc
    23041 gctttcaggt tgtcagtacc gccaaccgca gcaatatagt tggttgccag ttgagtcaga
    23101 ccttcttcag tattgctgtt agcctcttca gttacgatct cgtcttcttt atcttcacga
    23161 cccggcgttt tcaggttgaa catgcggata accaaactga acaccacgaa gtagatagcg
    23221 aagaagacaa cacccatcac cagcagcatc cagacgttct ggctggcggc cggcaggtta
    23281 tacatcaacg cgtagtcgat agcccccgca gagaaagaga agccagcatg gatacccagc
    23341 agcgttgcca caaacaggct gataccggtc agcagtgcgt gcaggaggta cagcagcgga
    23401 gcaaggaaca tgaacaggaa ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca
    23461 gcaacagaaa gcagcatccc gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac
    23521 atcgccagcg ccgcacccgg cagaccgaac atcatgatcg ggaagaagcc ggacatgaac
    23581 atccccgcgg tgccgtcacc agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc
    23641 gccgcgttgg tgaattcacc aatctggaac caggcgatgg tgttcagcac ctgatgcaga
    23701 ccggttggga tcagcagacg gttgatgaaa ccaaagatac cggaacccag cgcgcctgca
    23761 gaaacgatcc actcgccgcc tgcatggata gcgtgctgta ctggcggcca gacgtaacca
    23821 aaaatggccg ccagcaccag gcagaaaaag ccggtggcaa tcggcacaaa gcgtttgccg
    23881 ccgaagaagc tcaggaagtc cggcagttta atatcggacc aacggttata ggctgcgcca
    23941 ccaaccagac cggtaatgat acccgccagt acacccatgt taatttctgg gttgatggtc
    24001 accatcgctt tggttaacac aaagtaacct accgcacccg ccagcgccgc cgcacctgcg
    24061 ctgtctttcg accagctgga tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa
    24121 atcgcaccgc ccgcctgggc aataaacgca acgttaagta aatctggctg accgaatcgc
    24181 agcaacagtg ccgccaccgg cagcaccgcg atagggagct gtaacgccct accgagtcgc
    24241 tggaaaaaac ctaaaatatt catcttattc cccctacgag aaccctattt ggctcgttta
    24301 aagccgtatt tttattttgc tgcaaattgt actgccgatg ttctgtaatc agattgttag
    24361 atcatctgct acagagtgtg tgaaaattta attcgtatcg caaattaaac gcgtgccttt
    24421 tgtgagtttt gtcaccaaat atcgttatca tcactccctt ttactggcta aagcagaaaa
    24481 cttattttat cattcaaaaa atcaggtcgg attgacgcct gtctgcgcaa atccaggtta
    24541 cgcttaaaga tgcctaatcc gccaacggct tacattttac ttattgaggt gaataatgag
    24601 actgatcccc ctaactaccg ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa
    24661 tcgtatcaat gcgttcaaac cgactgccga tcgtccgttt gttctgggcc tgccgactgg
    24721 cggcacgccg atgaccacct ataaagcgtt agtcgaaatg cataaagcag gccaggtcag
    24781 ctttaagcac gttgtcacct tcaacatgga cgaatatgtc ggtctgccga aagagcatcc
    24841 ggaaagctac tacagcttta tgcaccgtaa tttcttcgat cacgttgata ttccagcaga
    24901 aaacatcaac cttctcaacg gcaacgcccc ggatatcgac gccgaatgcc gccagtatga
    24961 agaaaaaatc cgttcttacg gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg
    25021 tcatattgca tttaacgaac cggcgtcttc tctggcttct cgtactcgta tcaaaaccct
    25081 gactcatgac actcgcgtcg cgaactctcg tttctttgat aacgatgtca atcaggtgcc
    25141 aaaatatgcc ctgactgtcg gtgttggtac attgctggat gccgaagaag tgatgattct
    25201 ggtgctgggt agccagaaag cactggcact gcaggctgca gttgaaggtt gcgtgaacca
    25261 catgtggacc atcagctgtc tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc
    25321 ttccaccatg gagctgaaag ttaagacttt aagatatttc aatgaattag aagcagaaaa
    25381 tatcaaaggt ctgtaattgt tatccctgcc ctggctcctt gctcagggca atatttttta
    25441 aaatcggggg gcagaatgta tgcattaacc cagggccgga tctttaccgg ccacgaattt
    25501 cttgatgacc acgcggttgt tatcgctgat ggcctgatta aaagcgtctg tccggtagcg
    25561 gaactgccgc cagagatcga acaacgttca ctgaacgggg ccattctctc ccccggtttt
    25621 atcgatgtgc agttaaacgg ctgcggcggc gtacagttta acgacaccgc tgaagcggtc
    25681 agcgtggaaa cgctggaaat catgcagaaa gccaatgaga aatcaggctg tactaactat
    25741 ctgccgacgc ttatcaccac cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc
    25801 gagtacctgg caagacatcc gaatcaggcg ttaggtctgc atctggaagg tccgtggctg
    25861 aatctggtaa aaaaaggcac ccataatccg aattttgtgc gtaagcctga tgccgcgctg
    25921 gtcgatttcc tgtgtgaaaa cgccgacgtc attaccaaag tgaccctggc accggaaatg
    25981 gttcctgcgg aagtcatcag caaactggca aatgccggga ttgtggtttc tgccggtcac
    26041 tccaacgcga cgttgaaaga agcgaaagcc ggtttccgcg cggggattac ctttgccacc
    26101 catctgtaca acgcgatgcc gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc
    26161 ctcgacgaag ctgacattta ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc
    26221 aacattcgta acgctaaacg ccttaaaggc gacaaactgt gtctggttac cgacgccacc
    26281 gcgccagcag gtgccaacat tgaacagttc atttttgcgg gtaaaacaat atactaccgt
    26341 aacggacttt gtgtggatga gaacggtacg ttaagcggtt catccttaac catgattgaa
    26401 ggcgtgcgta atctggtcga acattgcggt atcgcactgg atgaagtgct gcgtatggcg
    26461 acgctctatc cggcgcgtgc gattggcgtt gagaaacgtc tcggcacact cgccgcaggt
    26521 aaagtagcca acctgacctc attcacacct gattttaaaa tcaccaagac catcgttaac
    26581 ggtaacgagg tcgtaactca ataagagaaa gtatgacacc aggcggacaa gctcagatag
    26641 gtaatgttga tctcgtaaaa cagcttaaca gcgcggcggt ttatcgcctg attgaccagt
    26701 acgggccaat ctcgcggatt cagattgccg agcaaagcca gcttgccccc gccagcgtaa
    26761 ccaaaattac gcgtcagctt atcgaacgcg ggctgatcaa agaagttgat cagcaggcct
    26821 ccaccggggg ccgccgcgct atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg
    26881 gcgtacggct tggtcgtcac gacgccacca tcaccctgtt tgatctcagc agcaaagtgc
    26941 tggcagaaga acattacccg ctgccggaac gtacccagca aacgctggaa catgccctgt
    27001 tgaatgccat tgctcagttt attgatagct accagcgcaa actgcgcgag ctgatcgcga
    27061 tttcggtgat cctgccaggg cttgttgacc cggacagcgg caaaattcat tacatgccgc
    27121 atattcaggt agaaaactgg gggctggtag aagctctgga agaacgtttt aaagtgacct
    27181 gtttcgttgg tcacgatatc cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc
    27241 aggattgcga agactccatt ctggtgcgtg tccatcgcgg aaccggggcc gggattatct
    27301 ctaacgggcg catttttatt ggccgcaacg gtaacgtcgg tgaaattggc catattcagg
    27361 tcgaaccgct gggtgaacgc tgccactgcg gcaactttgg ctgcctggaa actatcgccg
    27421 ccaacgctgc cattgaacaa cgggtgttga atctgttaaa gcagggctac cagagccgcg
    27481 tgccgctgga cgactgcacc atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagtc
    27541 tggcgtcgga agtaattgag tatgtcggtc gtcatctggg taaaaccatc gccattgcta
    27601 tcaacttatt taatccgcaa aaaattgtta ttgccggtga aatcaccgaa gccgataaag
    27661 tgctgctccc tgctattgaa agctgcatta atacccaggc gctgaaggcg tttcgtacta
    27721 atctgccggt ggtacgttct gagctggatc accgctcggc aatcggcgct tttgcgctgg
    27781 taaaacgcgc catgctcaac ggtattttgc tccagcattt gctggaaaat taatgtgctt
    27841 ttatagtggc gcttattgtt gtcaatattc tgggtagtcc atgaccatta aaaatgtaat
    27901 ttgcgatatc gacggcgtgc tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt
    27961 tttgcacggg attatggata aaggcctgcc gctggtgttg ctgaccaact atccttcgca
    28021 gaccgggcaa gatctggcga accgctttgc cactgccggt gtcgatgtgc cggatagcgt
    28081 gttttatacc tctgcgatgg cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc
    28141 atatgtggtg ggcgaaggcg cactgattca tgaactgtac aaagccggtt tcactattac
    28201 cgatgtgaac cctgatttcg tgattgttgg cgaaacgcgt tcctacaact gggacatgat
    28261 gcataaagcg gcctatttcg tcgctaacgg tgcacgcttt atcgccacca acccggacac
    28321 ccacgggcgc ggtttttatc ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc
    28381 cgggcgcaaa ccgttctatg ttggtaagcc cagcccgtgg atcatccgcg cagcattaaa
    28441 caaaatgcag gcgcattcgg aagaaacggt gattgtcggc gataacttgc gcaccgacat
    28501 tctggcgggc ttccaggcag gtctggaaac gattctggtg ctttctggtg tttcatcgct
    28561 cgacgatatc gacagtatgc cgttccgccc aagctggatt tacccgtcag tcgctgaaat
    28621 cgacgttatc tgaataccac ccacgtctgc ggacgtggtt ggctctgatg acaaacgcca
    28681 aactgcctga tgcgctacgc ttatcaggcc tacgcagctc ctgcaatata ttgaattttc
    28741 atgcttttgt aggccggata aggcgttcac gccgcatccg gcatgaacaa atcgcacttt
    28801 atcagcaatt tggcc
//
LOCUS       DAZOXR010000038        28679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZOXR010000038 DAZOXR010000000
VERSION     DAZOXR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.038"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1046)
                     /locus_tag="ACHUNN_001409"
     CDS             complement(<1..1046)
                     /locus_tag="ACHUNN_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0575824.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSILPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="ACHUNN_001410"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="ACHUNN_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="ACHUNN_001411"
     CDS             complement(2433..3281)
                     /locus_tag="ACHUNN_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0575825.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="ACHUNN_001412"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="ACHUNN_001412"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0575826.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4183..4686
                     /gene="tadA"
                     /locus_tag="ACHUNN_001413"
     CDS             4183..4686
                     /gene="tadA"
                     /locus_tag="ACHUNN_001413"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0575827.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="ACHUNN_001414"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="ACHUNN_001414"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0575828.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="ACHUNN_001415"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="ACHUNN_001415"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0575829.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="ACHUNN_001416"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="ACHUNN_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0575830.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="ACHUNN_001417"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="ACHUNN_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0575831.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="ACHUNN_001418"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="ACHUNN_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0575832.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="ACHUNN_001419"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="ACHUNN_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0575833.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="ACHUNN_001420"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="ACHUNN_001420"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0575834.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="ACHUNN_001421"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="ACHUNN_001421"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0575835.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="ACHUNN_001422"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="ACHUNN_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="ACHUNN_001423"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="ACHUNN_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="ACHUNN_001424"
     CDS             22669..23652
                     /locus_tag="ACHUNN_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR0575836.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="ACHUNN_001425"
     CDS             23675..25186
                     /locus_tag="ACHUNN_001425"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0575837.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="ACHUNN_001426"
     CDS             25211..26209
                     /locus_tag="ACHUNN_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0575838.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="ACHUNN_001427"
     CDS             26242..27336
                     /locus_tag="ACHUNN_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0575839.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="ACHUNN_001428"
     CDS             27348..28220
                     /locus_tag="ACHUNN_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0575840.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28679)
                     /locus_tag="ACHUNN_001429"
     CDS             complement(28268..>28679)
                     /locus_tag="ACHUNN_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0575841.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6818 a         7093 c         7790 g         6978 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg caagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtatggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagacg ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaaactct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacgt
//
LOCUS       DAZOXR010000039        28264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZOXR010000039 DAZOXR010000000
VERSION     DAZOXR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.039"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUNN_001430"
     CDS             complement(<1..215)
                     /locus_tag="ACHUNN_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0575842.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUNN_001431"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUNN_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0575843.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUNN_001432"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUNN_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0575844.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUNN_001433"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUNN_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0575845.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUNN_001434"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUNN_001434"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0575846.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUNN_001435"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUNN_001435"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0575847.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUNN_001436"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUNN_001436"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0575848.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUNN_001437"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUNN_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0575849.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUNN_001438"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUNN_001438"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0575850.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUNN_001439"
     CDS             6526..6744
                     /locus_tag="ACHUNN_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0575851.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUNN_001440"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUNN_001440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0575852.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUNN_001441"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUNN_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0575853.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUNN_001442"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUNN_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR0575854.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUNN_001443"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUNN_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0575855.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUNN_001444"
     CDS             10175..12088
                     /locus_tag="ACHUNN_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0575856.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUNN_001445"
     CDS             12088..13110
                     /locus_tag="ACHUNN_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0575857.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUNN_001446"
     CDS             13104..13322
                     /locus_tag="ACHUNN_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0575858.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUNN_001447"
     CDS             13376..14245
                     /locus_tag="ACHUNN_001447"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0575859.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUNN_001448"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUNN_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0575860.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUNN_001449"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUNN_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0575861.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="ACHUNN_001450"
     CDS             15689..17779
                     /locus_tag="ACHUNN_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0575862.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUNN_001451"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUNN_001451"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0575863.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUNN_001452"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUNN_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0575864.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUNN_001453"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUNN_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0575865.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUNN_001454"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUNN_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0575866.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUNN_001455"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUNN_001455"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0575867.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUNN_001456"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUNN_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR0575868.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUNN_001457"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUNN_001457"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0575869.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUNN_001458"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUNN_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0575870.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUNN_001459"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUNN_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0575871.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUNN_001460"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUNN_001460"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0575872.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6840 a         7593 c         7129 g         6702 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tctt
//
LOCUS       DAZOXR010000040        28214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZOXR010000040 DAZOXR010000000
VERSION     DAZOXR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.040"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHUNN_001461"
     CDS             <1..468
                     /locus_tag="ACHUNN_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575873.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHUNN_001462"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHUNN_001462"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0575874.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHUNN_001463"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHUNN_001463"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0575875.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUNN_001464"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUNN_001464"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0575876.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHUNN_001465"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHUNN_001465"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0575877.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHUNN_001466"
     CDS             complement(4084..5313)
                     /locus_tag="ACHUNN_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0575878.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHUNN_001467"
     CDS             complement(5377..5817)
                     /locus_tag="ACHUNN_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0575879.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHUNN_001468"
     CDS             complement(5922..6692)
                     /locus_tag="ACHUNN_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0575880.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHUNN_001469"
     CDS             complement(6689..7615)
                     /locus_tag="ACHUNN_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0575881.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHUNN_001470"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHUNN_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0575882.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUNN_001471"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUNN_001471"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0575883.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUNN_001472"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUNN_001472"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0575884.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUNN_001473"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUNN_001473"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0575885.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHUNN_001474"
     CDS             13322..13669
                     /locus_tag="ACHUNN_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0575886.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHUNN_001475"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHUNN_001475"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0575887.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHUNN_001476"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHUNN_001476"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0575888.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUNN_001477"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUNN_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0575889.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUNN_001478"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUNN_001478"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0575890.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHUNN_001479"
     CDS             18978..20735
                     /locus_tag="ACHUNN_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR0575891.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUNN_001480"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUNN_001480"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0575892.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUNN_001481"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUNN_001481"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0575893.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUNN_001482"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUNN_001482"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0575894.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUNN_001483"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUNN_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0575895.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6800 a         7677 c         6937 g         6800 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaatt
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attg
//
LOCUS       DAZOXR010000041        27786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZOXR010000041 DAZOXR010000000
VERSION     DAZOXR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.041"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..317
                     /gene="tnpA"
                     /locus_tag="ACHUNN_001484"
                     /pseudo
     CDS             1..317
                     /gene="tnpA"
                     /locus_tag="ACHUNN_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            377..451
                     /locus_tag="ACHUNN_001485"
                     /pseudo
     CDS             377..451
                     /locus_tag="ACHUNN_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(448..1771)
                     /locus_tag="ACHUNN_001486"
                     /pseudo
     CDS             complement(448..1771)
                     /locus_tag="ACHUNN_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1771..3387)
                     /locus_tag="ACHUNN_001487"
     CDS             complement(1771..3387)
                     /locus_tag="ACHUNN_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0575896.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3683..4399
                     /locus_tag="ACHUNN_001488"
     CDS             3683..4399
                     /locus_tag="ACHUNN_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0575897.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4396..6057)
                     /locus_tag="ACHUNN_001489"
     CDS             complement(4396..6057)
                     /locus_tag="ACHUNN_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0575898.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="ACHUNN_001490"
     CDS             complement(6253..6681)
                     /gene="ibpB"
                     /locus_tag="ACHUNN_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0575899.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="ACHUNN_001491"
     CDS             complement(6793..7206)
                     /gene="ibpA"
                     /locus_tag="ACHUNN_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0575900.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7200..7272)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7512..7844
                     /locus_tag="ACHUNN_001492"
     CDS             7512..7844
                     /locus_tag="ACHUNN_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0575901.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7846..9060)
                     /locus_tag="ACHUNN_001493"
     CDS             complement(7846..9060)
                     /locus_tag="ACHUNN_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0575902.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9161..10225
                     /gene="cbrA"
                     /locus_tag="ACHUNN_001494"
     CDS             9161..10225
                     /gene="cbrA"
                     /locus_tag="ACHUNN_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0575903.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10222..11559)
                     /locus_tag="ACHUNN_001495"
     CDS             complement(10222..11559)
                     /locus_tag="ACHUNN_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0575904.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="ACHUNN_001496"
                     /pseudo
     CDS             complement(11634..12782)
                     /gene="dgoD"
                     /locus_tag="ACHUNN_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="ACHUNN_001497"
     CDS             complement(12779..13396)
                     /gene="dgoA"
                     /locus_tag="ACHUNN_001497"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0575905.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="ACHUNN_001498"
     CDS             complement(13380..14258)
                     /gene="dgoK"
                     /locus_tag="ACHUNN_001498"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0575906.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="ACHUNN_001499"
     CDS             complement(14255..14944)
                     /gene="dgoR"
                     /locus_tag="ACHUNN_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0575907.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14913..15545
                     /locus_tag="ACHUNN_001500"
                     /pseudo
     CDS             14913..15545
                     /locus_tag="ACHUNN_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15591..16403)
                     /gene="yidA"
                     /locus_tag="ACHUNN_001501"
     CDS             complement(15591..16403)
                     /gene="yidA"
                     /locus_tag="ACHUNN_001501"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0575908.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16518..16916)
                     /locus_tag="ACHUNN_001502"
     CDS             complement(16518..16916)
                     /locus_tag="ACHUNN_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0575909.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17156..19570)
                     /gene="gyrB"
                     /locus_tag="ACHUNN_001503"
     CDS             complement(17156..19570)
                     /gene="gyrB"
                     /locus_tag="ACHUNN_001503"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0575910.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19599..20672)
                     /gene="recF"
                     /locus_tag="ACHUNN_001504"
     CDS             complement(19599..20672)
                     /gene="recF"
                     /locus_tag="ACHUNN_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0575911.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20672..21772)
                     /gene="dnaN"
                     /locus_tag="ACHUNN_001505"
     CDS             complement(20672..21772)
                     /gene="dnaN"
                     /locus_tag="ACHUNN_001505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0575912.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21777..23180)
                     /gene="dnaA"
                     /locus_tag="ACHUNN_001506"
     CDS             complement(21777..23180)
                     /gene="dnaA"
                     /locus_tag="ACHUNN_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0575913.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23477..23557
                     /locus_tag="ACHUNN_001507"
     CDS             23477..23557
                     /locus_tag="ACHUNN_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575914.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23787..23927
                     /gene="rpmH"
                     /locus_tag="ACHUNN_001508"
     CDS             23787..23927
                     /gene="rpmH"
                     /locus_tag="ACHUNN_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0575915.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23944..24303
                     /gene="rnpA"
                     /locus_tag="ACHUNN_001509"
     CDS             23944..24303
                     /gene="rnpA"
                     /locus_tag="ACHUNN_001509"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0575916.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24267..24524
                     /gene="yidD"
                     /locus_tag="ACHUNN_001510"
     CDS             24267..24524
                     /gene="yidD"
                     /locus_tag="ACHUNN_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0575917.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24527..26173
                     /gene="yidC"
                     /locus_tag="ACHUNN_001511"
     CDS             24527..26173
                     /gene="yidC"
                     /locus_tag="ACHUNN_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0575918.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26279..27643
                     /gene="mnmE"
                     /locus_tag="ACHUNN_001512"
     CDS             26279..27643
                     /gene="mnmE"
                     /locus_tag="ACHUNN_001512"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0575919.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6685 a         7520 c         7090 g         6491 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttta aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtcctggct
      241 attttgccaa tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
      361 ttacggcgca ccggataaat ttggttatgc gccgtcagcc gtgctaaaga atacggacca
      421 gcacccaacg gatgccagtc cacacaatta acgtaactct ggccagtacg ctttgttggc
      481 ctccagcaac tcatccagaa tagctttggc gaccgaggcg ctcggtaccg ttttcgataa
      541 cgtaatcgcc tgccacaggt gctgatatga acgctgttcc caggcgtcca cgaccagttt
      601 ttccaccgcc acttgctgac tcattaaccc tttctgaaac tgcgggatat cgccgaccac
      661 cagtggttct ggtccgttgt gcccgaccag acacgggatc tcgaccatcg cttcatcatc
      721 aaagttatgg attgccccgt tgttaggcac aatcagcaac atccgctcct gagtgttgaa
      781 ggcaattgcc gccgccagat cgacgatgta tgacgcatgt tcgtcaatct ccagcttggc
      841 cgccgctgaa tttcctgccg ctgtaatggc gcggcaagca tcgaaaacct gtttctcgcg
      901 atgttccatt cacctcattc gcgcgggtat gttcagggtt ggaatgctga acaacataat
      961 ccgggaagag ataatatttc agataggtgt tcggcaatgt atccgggtcc agtgcctgga
     1021 catcccgcgc ttttgcatag gtgtcgttcc agctcgcttc agtatgtgta tcttgctgcg
     1081 gtggaacata accgtgcttc gagacatatt gccgcagctg gggcatcagg tcgttgccct
     1141 gcaaatcttc aattgatgtc caccagccaa agtggttcag gccgtagtag cgcacgcgca
     1201 tctgtttgcg atcttgcagc ccaacaattt gcgccatccg gctttcaata ccgattggca
     1261 tgtcacagat gttgaggatt ttcgcattcg ggcgcagacg acgcgtggct tctgcgacaa
     1321 tggctgccgg gttggagtag ttgagcatcc aggcatttgg tgaatatttt tccatataat
     1381 ccaccagttc caggacgccg ccaatggaac gcatgccgta cgctattccg ccgggtccgc
     1441 aagtttcctg accaacaaca ccgtggcgca gtgggatttt ttcatccagt tcgcgcatcg
     1501 ggtacttgcc gacgcggatg tgcgccatga caaaatcaac gtcgctgaac gccacttcag
     1561 gatcggtggt gtaactaaac gcaatgtccg gcgctttctc tttaaggatg actttacagg
     1621 cttcggcaat cacttcctgc cgcgcgccat cgttatcata aaatttcagt gcacgaagcg
     1681 ggaaacgctc ctgattcgcc aggagcatca acacgatgcc tggcgtaaag gtgcttccac
     1741 cgcctgcgac aaccactgag aatttggtca ttatactgcc tccgtaatgg caacattttc
     1801 tgctgattga tgagaattaa ttaagctatc gagctgttca cgcagctggg atacatgcag
     1861 accaatgatc acctgaatgg catcgccact acggaagacg ccgtgcgctc ccagcttttt
     1921 aaagacttcg tcatccagcg tttgtgacat gtcatgcagt gcaatgcgta gacgcgtcgc
     1981 acaattgtta atgctggaga tattgccgac cccgcccagg gcttgcagga taccggcagc
     2041 ctgatccagc tctttttttg gctctgccgc ggtggtttgg cctcgcgagg ctttgtattc
     2101 ggcttttgag tagagtttca cttccgcatc ttcacgtccc ggcgtgcaca tgttgaactg
     2161 cagaatcagg gtgcggaaaa ccacgaagta cagcagggta aagcacaacc caatggcgat
     2221 ttgggtcagc atcatatccg cgtggttgct gaacatcggg atccagtttt gcggtaaaac
     2281 ctggtcaatc agacctccgc ccatgttgcc caccacacca aagagataca tcacggtcga
     2341 cattgaggcc gcaagcacag cgtgtaccgc aaacagcaac ggtgaaatga acaggaaggt
     2401 aaattccagc ggttcggtaa ttcccaccag catggcggtt aaggttgcgg gaatcagcaa
     2461 gcccgccact tttacccgat tttccggtgc ggcagtgaag tacatcgcta aagaaatgcc
     2521 cacggcacca aagatttttg agttaccgtg cagggcaaaa ccgccttccg ggaacaacga
     2581 tttcagcggc tcggcgctca aactgaactc ttgcagatgc tgcgcccagt acatctgaat
     2641 gccgccttca acagctgccg gacccaagat aaacggtccg tagatgaagt ggtgtaaacc
     2701 ggttgggatc agaatacgtt cgaggaaggt gtaaacccag accccaagcg cacccgccga
     2761 acgcaggaac gcttgcagag attcaatccc cgtttgtact tttggccagc cgagcaatgt
     2821 cagccaggca caggggatca tcaccaggaa ggcgataatc accacataag acgtcccctg
     2881 gaaaatgccg agaaaaaccg gcagtttttt atcgaacaga cggttatgca gcgccgtcac
     2941 aatgccggaa atgataattg cgccgataat gctggtatcg agggttttaa tcccggccat
     3001 cattgtcaga ccgctacctg ccaccgcgtc ctgagtgaaa tcgacgccga agtagcttcc
     3061 ccaggtcatt cccatcgcgt tgatgaaata gttccaggtc aggaaactca ccatcaccgc
     3121 cagacaagca cgtccctgcg cttgcttagc aaggccaatg ggtaaaccga cagcaaaaat
     3181 cagcggcata ttacggaata ccgtccaacc gccctcttca ataatgtgta cgatttgcgc
     3241 gaataaactg ttcggatcgg tcagtgattc cccgacaaac atcgggtttt gcagcaagat
     3301 ggcaagaccc accacaatcc cggcgaaggg aaacagcagc actggcgtaa acatcgcgcc
     3361 gccaaagcgt tgaatttgac tgagcatttc cacatcctct tttctcaatt ctgtaagtag
     3421 gaagcctgat tggttatgcg gcaggcctgg agcaaggata ggaagatgcg atcggcagaa
     3481 catgacgacc caatgggatt cgtgatcgct ttcatgcttt tcgttgggcg gtagagatct
     3541 acgttttcgc gaaaaagtag acatctgtga gcacaggtac acagcatcga acacatcttt
     3601 aaaaaaaaga tgttttttca atcgattaag cagaacttgt gtgcgcatta cccgggcttg
     3661 caggcaaaaa agagatctag agatgatcta caaaagcatt gcggagcggt taagaattcg
     3721 acttaactcc gcagatttca cgctaaacag ccttcttccg ggtgaaaaaa agctggcgga
     3781 agagtttgcg gtatcgcgga tgaccatccg taaagccatt gacctgctgg tagcgtgggg
     3841 gctggtggtc cgccgccacg gtagtggcac ttacctggtg cgcaaagatg tactgcatca
     3901 aaccgccagt ctgaccgggc tggtggaggt gttaaaacgg cagggaaaaa ctgtcaccag
     3961 ccaggtgctg atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat
     4021 tcaaatcaac gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct
     4081 gatgctggaa gacagctata tgccggtaaa actgttccgt aatctttcgc tgcaacatct
     4141 ggaagggtcg aagtttgaat atattgaaca agagtgcggg attttgattg gcggtaatta
     4201 tgaaagcctg acgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga
     4261 acacacgcca ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa
     4321 ttattcagtg atgttcagaa atgccagcga ataccaggtg gagtaccatt tacggcgact
     4381 ccacccggaa aagagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat
     4441 aatgcgcagg aacatcacta acggatagac agtggcgtaa gagagcgccg ccgcaccgct
     4501 agttggatga agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag
     4561 catcccgcac atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt
     4621 aatcagcgga acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt
     4681 gaccagggta ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac
     4741 gatccccagc tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc
     4801 gatactgccg atacgcccga ggatcaacgc cataatcagc agaccgcccg ccagccccag
     4861 tttcaacgcg gccgggaatc ccggcacaaa gacgggaata gaacctaaca atacgccaag
     4921 cccgatgcca ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc
     4981 cagcacattg gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc
     5041 gaactgcagg ctgatatcgc cactggcgac cagttcgacc ccggcacggt tcaggcgcga
     5101 gataacaacg tcatagcgtt ctttaaagtg caggtcgcga atacgttttc cgagcacgtt
     5161 ttcattggtg accaccacac gctcgacgcg caaatcagtg tctttcgtgg acagcgaagt
     5221 atcgacctcc tgaccaatca ccagttgcgc attatgtaaa tccgctggct gacccaccag
     5281 atgcagcaaa tcgcccagtt ggataatggt atctggcgaa ggaactttta gggtttcttc
     5341 gcgtttcaga cgcgagcaga taattttgtc gccgttgaga atcggtacat ctttaatggc
     5401 taaatcatgc aggttagggt tctcaacgcg aatattgata gtcttgatca gcgcgccgcc
     5461 attggtgcgt gaagactcgt gctgctgagc ttctgtctcg acattgacgc ggaaaataac
     5521 ccgcaacatc cacatggtga acaaaatccc gcaaatgccg aatggatacg ccatcgcgta
     5581 actcatcccc atctgatcga ccatttccat tggtgtaccc aggtcgcgca aaatctgctg
     5641 ccctgccccc agcgctggcg tattggtaac cgcaccggag aaaatcccca gcactaccgg
     5701 cagtggaata tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat
     5761 cagaacagca aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg
     5821 ccctacctga atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg
     5881 cagcatatcg ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc
     5941 accaaacagc acgccgccaa tacctaatcc tatgccgcga aatttgacgt tgccgataaa
     6001 caaaccgacg actgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt
     6061 ccatttctct tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga
     6121 tttgccttat agcgcacatt attacgggaa agaacgtgcc gaaatatctt aaacagtcga
     6181 aatttaacgc attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa
     6241 gagaatagct agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc
     6301 gatgggttca ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaagttgc
     6361 gccagagact tccatatttt cagccagcgt aaagctcagg ctaaatggct gattcataag
     6421 cccttgatgc agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag
     6481 gcgcgtacct tccagttgaa tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt
     6541 aatgcggtag tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctgactttc
     6601 accggcgttt tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg
     6661 ggataaatcg aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa
     6721 atccgaagat tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc
     6781 ggccttaagg aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg
     6841 ttcgagatcg atatacagca aaccatttac caggttagcg ccacgaacat gaatgttctc
     6901 agctaactgg aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg
     6961 ctctttttgt tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat
     7021 ttccagttcg ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc
     7081 taccagttca acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa
     7141 gtggttaaac aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt
     7201 acgcataatc aatagctcct gaaatcagcg agaatgtaag accttccaca atggacaggt
     7261 caggtagcca gaacacccat caggcgcgtc ctcatcgact acgatgtaaa aatgggtctg
     7321 gaaatgaact tttcaagcct tatcagcgga cttttttttg cagtttatgg tctattgcat
     7381 agactgaggg ggcagcacga aacgttaaag tgtgatagcc gccacagagc gacgtaatga
     7441 tgatgttatt ttcagcaagg gatcgctata actcatcagg ccaacaccca cgatcaacag
     7501 atgaatttat tatgataaga aatgtgttgt tagcgttcat gatatgcagc ggaatggcat
     7561 tactcggagg atgctccagc gtgatgtccc ataccggagg gaaagaagga acgtatcccg
     7621 gcacccgcgc cagcgccacc atgattggcg atgatgaaac caactggggc actaagtcgc
     7681 tggcgattct cgatatgccg tttaccgctg tgatggacac gcttttatta ccgtgggatg
     7741 tgttccgcaa agacagctcg gtgcgctcac gcgttgaaaa aagcgaagcc aacgcgcaag
     7801 ccaccaatgc ggttattcca ccggccagaa tgcctgataa ctgatttagc gatcagtttc
     7861 ggtgatccac agctggccac cttccatcca cgccagccat tgaccatccg gcgagaagac
     7921 cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt aaatactcaa cctcgccgct
     7981 ttgcgcatgg gcacaagcaa ttcgattatc cagcacaaag cccaaccatt ctcctgacgg
     8041 atgccagtta aatgcagact gaatatccgt tttgttatgg gttaactggc gcggctcgcc
     8101 gccctgtggc gagataagcc acagttgcac aatgccgtta tcatcacgca ttaaaaacgc
     8161 gatttgcgta ccctgcggat tacagcgcac ccagtggcgc gggacgttga ctaaccccgg
     8221 ataagcccga tggtgggtaa aggttaaacg tcgctgcacg acgccacgcg gtggcgcggg
     8281 cagggtggtt tccgttccac ttaacggcgc atcacctgcc gctttccagc cagcttcatc
     8341 ttgcggcaac tcaacgataa acagctccgg cactttctca ccctttggcg aaagtgtgtc
     8401 gccaataaac gccagcgcgt gatttcctac ccatccttct tcataagcac gattgatttc
     8461 atcactgcca ggctgcggcg tgggcgtggt tttactcacc agcacacacc agtggctgcc
     8521 gctgtattca cgcggatgct gcttttgtac gttgaccgga ccaaacggcg cagcaacacc
     8581 gacgtttcgc aaatccagcg ccggatcgag ttcatgcatt acatggtcgt tataggtaaa
     8641 gctcaccctt tcaccgttcg ggctaaagac atgcacatgg ctgccgccgc gcagcgctcc
     8701 tggcgtatac ggcgcggtaa tatccattgc atcgagattg ctcaccttgc cgccttcagc
     8761 aatgactccg cgccgatgat ggaagtcgta atgccatgtt tcatcaggat tttccgggcc
     8821 gtgaataaag acatatttct ctgacttcgg atgaacggtc accacgccga cgtgtgcgcc
     8881 ctgtgacgca cagtagataa cctcgacctc accggtatgg atattcacac gctcaatggt
     8941 ctcgccggta aacgacgccc cagaaggacg cacgtcaaat accagccact ggctgtcggg
     9001 cgtccaggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta tctgtttcat
     9061 ggcgcggtcc tgatacaaaa cgggtccagc catcatactt cacaaggact tcactttcag
     9121 acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt tcgacgtggc
     9181 gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg gcaaaatgca
     9241 ggtgatcgcg ctggataaaa agcaccagca tggtactgaa ggtttcagca aaccttgtgg
     9301 cggtctgctg gcaccggacg cgcagcgatc ttttattcgc gatgggctga cgcttcctgt
     9361 cgatgtgatc gctaatccgc agattttcag cgtcaaaacc gtcgacgtcg ccgcatcgct
     9421 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
     9481 gaaatcgctg atccccgcca gcgttgaggt ttatcacgac agcctgtgtc gaaaaatctg
     9541 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc agcatattac
     9601 cgcccgctat ctggtcggtg ccgatggtgc caactcgatg gtgcggcgac atctctaccc
     9661 ggatcatcaa atccgtaaat atgtcgcaat ccagcagtgg ttcgcagaga aacatccggt
     9721 gccgttctac tcctgcatct ttgataatgc gataactgac tgttattcat ggagtatcag
     9781 caaagacggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
     9841 cacgactctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
     9901 cgaaaaatgc acggtgctgt ttccctcacg ctggcaggat tttgtctgcg gtaaggacaa
     9961 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    10021 ctatgcgctg gatagcgcag acattctgcg ttcggtgtta ctgaagcagc cagagaagct
    10081 caataccgct tactggcgcg ccacccgcaa actgcgttta aaactcttcg gcaagatagt
    10141 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    10201 acatattcca cagttgaagg attagccaac gcgcttcaca tcgcccacca gcaggatata
    10261 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggtg cgaaaccgta
    10321 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    10381 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag aaaccagcga
    10441 ccaggtaatt gaagcaaaac cgttaccgaa gaatgccagc gccatcaggc acataatcat
    10501 catcggatcg ttagtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    10561 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgctac
    10621 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    10681 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    10741 aaaccacagt gtagaagcca ccgcaaattg cccaagatag acgccgatca gtttacgatg
    10801 gaacaccagt ttccagtctt tggctgttaa cggctgacgt gcctctttct tcactggcgc
    10861 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatgcc
    10921 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    10981 accaccagtc acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    11041 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaggc
    11101 gcgttcatgt tccgggaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    11161 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    11221 ctggaataaa gtcgccactg accagccaaa tatcgcaata aaataagtca cgcgagaacc
    11281 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    11341 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    11401 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    11461 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    11521 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    11581 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    11641 tctgctacgc tgttatcttc atgacgccag agtggattac gccagtccgg ggcattttta
    11701 ctgaactcaa taactttagc ttcgtcgatt tccacgccta agcccggttt cgttagcggt
    11761 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    11821 gcgcctttgt tgtaatgaat tcccatactt tgttcctgaa gtaccgcgtt ataggaaaca
    11881 aagtcgatat gcagacaagc cgccagtgca atcggtccga gcggacagtg cggcgcaagg
    11941 gtcacgtcat aggcttctgc cattccggca attttgtagc attcggtaat accgcctgca
    12001 tgggagagat ccggttgtag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    12061 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    12121 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    12181 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    12241 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    12301 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    12361 tcgaagccga tttcgcgcag ggttttaata ccgtcgatca catccgccgg acgatcgccg
    12421 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagacc
    12481 gacgcattca gcactttacc tttgatatcc cataacgcct ggtcgatccc ggcaatggcg
    12541 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtca
    12601 ttgatgcgct atggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    12661 accgtgcggg cgcggccttc aatcactggc tcgccccagc cgacgacgcc ttcatcggtt
    12721 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    12781 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    12841 cggactgccc ggcgcgatag agatcgctgc ctaagcccgc cccagcacaa cctgcgtcta
    12901 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccgatg
    12961 gcaataccgc ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    13021 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    13081 ggcagacggt catgccgtag cccaccgcac ggcggatcac ttcactatgg atattgggcg
    13141 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    13201 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctttgct
    13261 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    13321 caacatgcgc cagcgcctcg tcgggcttaa tgccgcgcaa aatggcgatc agcggaagtt
    13381 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    13441 accgccgtca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcaacgat
    13501 gttccggcga caagggtgat ggcgtgttga tgggccacat agtcgcgcat actggcgacc
    13561 tctgcgccaa tcaacaaacc agagagaaat tcactgacct gttcacgggg aagtgttccc
    13621 agcacatgcg aggcacgaac ttcaaaaagc tgcggcaata tggcgggcgt attaagacca
    13681 cgctcaaggc cagccgtgaa ggcatcggca gagttttcct gcggcggcaa acctgcacca
    13741 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    13801 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat gacataaaga
    13861 gaggaaggag ccagagctcg cgcgccgatc aattgagttt cttcgccgcg catcacattg
    13921 tggttatcat catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    13981 gttaattgtt cgccaataga cgaaaaatgg gcaggaacag ataaatacgg agcaactttc
    14041 cagccgacat tgctgccgac cattcctgcc attaccactg gcgtattctc ttcacgccag
    14101 tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct
    14161 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    14221 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    14281 tcgagctggc gatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    14341 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    14401 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    14461 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    14521 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    14581 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    14641 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    14701 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcg ccgcgataac
    14761 gtttcatttc aatcagccgt ttcgccatca acgaacggaa cacctcacgg atgatgttgc
    14821 gcgaggttgc aaactcctca cagagttccg cctcagccgg aagcggcgag cctggtacgt
    14881 atttgccgtg aacgatctgt ttacccagcg taatgacgat gcgatcggtt ttattgagag
    14941 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataat
    15001 caggaaagta tcaatcgcag tcagacacca tcacggggct atcgcaggca aaggatagta
    15061 atggaacaaa aggctatgtt tttgtagggg aatcgctgga ttaccttatc acagatggtg
    15121 ccgatgacat cgttaagatg ctcaaggatc cagctcttaa ccggcacaat attcaggttg
    15181 ctgatgacgc aagatttgtt ttaaatgcgg ggaaaaagaa atttaccggc acaatatcac
    15241 tctattacaa ctggagcaca gaagaagaaa aagcacgagt gacacaatat cgttttgctt
    15301 gtggtgttca gcactgtacc cggacagtag aaaacctgga aggtactatc catgagaaaa
    15361 ataaaaacat ggattactca aacgtaatgg ctttctacca tccatttaaa gtgcgatttt
    15421 atgaatacca ttcacccaga ggcattccgg atggtgtttc cgcagcatta ctgccagtga
    15481 ctgttacgct ggacatcatt actgcaccgc tgcaatttct ggttgtatat gcagtaaacc
    15541 aataatcagt aagcgggcaa acgcgtttat gctgtttgcc cgcccacaga ttaattcagc
    15601 acatacttct caatagcaaa cgccacgcca tcttcaaggt tagatttggt gacaaagttc
    15661 gccacttctt tcaccgaagg aatagcgtta tccatcgcca caccgacgcc tgcatattca
    15721 atcattgcga tgtcgttttc ctgatcgcca atcgccatga tttcttccgg tttaatacct
    15781 aacacgtcgg ccagtgattt cacacccgta cctttgttaa cgcgtttatc gaggatttcg
    15841 aggaagtacg gcgcactttt cagcacggta tatttctctt tcacttcctg cggaatacgc
    15901 gcgatagcct ggtcgaggat ggcgggttca tcaatcatca tcactttcag gaactgggta
    15961 ttggggtcca ttttctccgc ttcgcagaac accagcggaa tagtggcaac gaaagattca
    16021 tgcactgtgt agtagctgat atcgcggttg gcggtgtaca gcgtggtgcg gtccagggcg
    16081 tggaaatgag aaccgacttc gcgagagagt ttttccagga aacgatagtc gtcatagctg
    16141 agagcagttt gcgccacggt gctaccatca gctgccttct gtactagcgc gccgttataa
    16201 gtaatgcagt agtcgcccgg ctgttccata tgcagctctt tcaggtagtt gtgcacacct
    16261 gcatacgggc gacccgtcgt tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc
    16321 gcatttttaa cggcgggtga aatggtgtga tcgggcagca gaagggtgcc atccatatcg
    16381 atagcaatga gtttgatagc catgagttcc ccaggtagat tggttcctga cccatgctaa
    16441 cgcgattccg ctcaaaaatc agtacaacac ccgagggaaa agggggatgc aacgcgggtt
    16501 gcatgctccc catttgctta gcggaagagt ttccctttca gcagttccat acctgcggaa
    16561 agcagatcgt tattggcttg tggtgacact tcaccttgcg gtgagagcgc atcaataatc
    16621 ttcggcaatt gctctgccag taaactggaa gctgtactgg tatccacgcc aagtttttgc
    16681 ccgagatcgg acaccgcatt tgtgccgagc gccgattcca gttgctcgcc actaaccgat
    16741 tgattgccct gttgattact cagccaggtt gagagaatgg cccctaagcc gccactttgc
    16801 agtttttcca gcagcacctg aatgccgccc tgctcctcaa cccaacttaa aatagcctga
    16861 tatttccccg catcgccttt cagaaaggca ccgacaactt catcaaaaag ccccatgata
    16921 atcacctgta aagcgttacg tgttgaccca aaaagtatag atttgcggga tgataattgc
    16981 ggattgcaga aataaaaagg gcggagatga tctccgccct tttcttatag cttcttgccg
    17041 gatgcggcgt gaacgcctta tccggcctac aaaatcatga aaattcaata cattgcaaga
    17101 ttttcgtagg cctgataagc gtagcgcatc aggcacgctc gcatggttag cgccattaaa
    17161 tatcgatatt cgccgctttc agggcgttct cttcaataaa cgcacggcgc ggttcaacgg
    17221 cgtcgcccat cagcgtggtg aacaactggt cggcagcaat cgcatcttta acggtaacgc
    17281 gcagcatacg acggctttcc gggtccatgg tggtttccca cagctgttcc gggttcatct
    17341 cgcccagacc tttataacgc tggatggaga ggccgcgacg ggactctttc accagccagt
    17401 ccagcgcctg ctcgaagctg gctaccggct gacgacgctc gccacgttcg ataaacgcat
    17461 cttcttccag caagccacgc agtttctcac ccagcgtgca gatacgacga tattcgccac
    17521 cggtgataaa ctcgtgatcc agcggatagt cagtatccac accgtgggta cgcacgcgaa
    17581 caatcggttc aaacaggttt tgttcggcat tggtgtggac atcaaacttc cactggctgc
    17641 cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg gtaacggtct
    17701 gctcatcaga gaggtcggct tccgtcagcg tcggctgata gataagctct ttcagcattg
    17761 ctttcggata acgacgctcc atgcggttga tcattttctg cgtcgcgttg tactcagaca
    17821 ccaatttctc taacgcttcg ccagccaatg ccggtgcgct ggcgttggtg tgcagcgttg
    17881 cgccatccag cgcgatagag atctggtact gatccatcgc ttcgtcgtct ttaatgtact
    17941 gttcttgctt gcctttcttc actttgtaca gcggcggctg agcgatgtag acgtgaccgc
    18001 gttcaacgat ttccggcatc tgacgataga aaaaggtcaa cagcagcgta cgaatgtgcg
    18061 agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt ttgtccgggt
    18121 tgtactcgtc acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc acttcctgag
    18181 aagagagcat cttatcgaag cgcgctttct caacgttgag gattttaccc ttcagcggca
    18241 gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg gagtcccctt
    18301 ccaccaggta cagttcggaa agcgccggat cgcgttcctg gcagtctgcc agtttgcccg
    18361 gcaggcccgc taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg cgacgcgccg
    18421 cttcacgggc acgggcagca tcgataattt tgccaaccac gattttcgcg tcggttgggt
    18481 tttccagcag gtattctgcc agcagttcgt tcatctgctg ttcaaccgct gatttcacct
    18541 cagaagaaac cagtttgtct ttggtctggg aggagaattt cgggtccggc actttcacgg
    18601 aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt ggcgctaact ttggcttttt
    18661 tgctgtagcc ttctttgtcc atgtaggcgt tcagggtacg ggtcatcgcc gcacggaagc
    18721 ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag tagatgtttt
    18781 cctggaagcc atcgttccac tgcaacgcca cttcgacgcc aataccgtct ttttcagtgg
    18841 agaagtagaa gatattcggg tggatcggcg ttttgttctt gttcagatat tcaacgaacg
    18901 ccttgatgcc gccttcatag tggaagtggt cttctttgcc gtcgcgcttg tcgcgcagac
    18961 gaatggaaac gccggagttg aggaacgaca actcacgcag acgtttcgcc agaatttcat
    19021 attcgaactc ggtcacattg gtgaaggttt cgaggctggg ccagaaacgc accatggtgc
    19081 cggttttttc agtctcgccg gtaaccgcca acggtgcctg cggtacaccg tgttcgtaga
    19141 tctgacggtg aattttaccc tcgcgctgga taaccagctc cagtttttgc gacagggcgt
    19201 ttactaccga aacaccaacg ccgtgcagac cgccggacac tttataggag ttatcgtcaa
    19261 atttaccgcc tgcgtgcaga acggtcatga tcacttccgc cgccgatacg ccctcttccg
    19321 ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac agagacagag ttatcggcgt
    19381 gaatggtgac gataatttct ttacagtgac ccgcgagcgc ttcgtcgata gcgttatcta
    19441 ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg atatacatac
    19501 ccgggcgctt acgcaccgca tccagccctt tcaggacttt gatactggag gagtcataag
    19561 aattcgacat caacgtttct cgctcattta tacttgggtt aatccgttat tttacccttt
    19621 tccacggtaa acatcttcga attttcgtcc gacatgtcta taacgtgttc agcactgatc
    19681 gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc tggcaagcag cccgcgacgc
    19741 tcatcatcaa gctcagaggc aaaatcatct atcaggtaga gacaccgccg cccgctttca
    19801 cgggtgagga actctccttg cgccagacgt aaggcgcaca tcaacagctt aagctgccca
    19861 cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc cgctttatgc
    19921 gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa aattacgttc cagcacctca
    19981 gcatattctg tctctttctc ccagccgcgc tggaaagaga aagtcagaga aaactcaggg
    20041 agaaattgct tacaagtatc ggccatatca gccgcgatac cggcgctata ctccgcgcgc
    20101 caggtgctga tttgctccgc cagcgggatc agctctttat cccacgggcg tagctgttcg
    20161 taacgtgtca cctggcgcag cgccgcattg cgctgcttga gcaatcgctt gagattgctc
    20221 caggcggtga aaaatccggg ttcgttgtga aagcatcccc agtcgaggaa tgctcttctg
    20281 tatttggggc cgccgttgag taaagtaaac ccttctggcg ttatcaactg cattggcatc
    20341 aggtgcgcca gttccgcgac cttatgcccg tctgtaccgt cgatgcggac tttgctgtcg
    20401 ccctgtttgt ctttggttaa gccaatcgct gtctcgcgct cttcgccctg taatcgcccg
    20461 tggagaacaa acgcctcctg ctcatggcga atgacgcgac caatctgcaa actgcgaaac
    20521 gcccgaccat ggccgagcgt atagatggct tccagcacgc tggttttgcc actgccgttg
    20581 gcacctacca gaaagttaaa gccgggagat aaagcgagat ccgcggtttc aatgttgcgg
    20641 aaatcgcgga tcaacaagcg ggtgagggac attacagtct cattggcatg acaacatagg
    20701 cagccgactg tgatgcggca tcttcaatct gcacgctgga aaccgaatcg gtcagcatca
    20761 tgcggacgtt ttcacatttc agcgcgttca gaacatccag cacgtagctg acgttgaagc
    20821 cgatttccat ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct tcttcctgtt
    20881 ccgggttgtt ggcggtgatt ttcagctggt tttcgctgac ataaagacgt acgccgcgga
    20941 atttctcgtt agagagaatc gccgcgcgag caaacgcctg cttgagcaga tcgcagccag
    21001 cttccagatg tttgtccggg ttcttcggca gaacgcggcg ataatccggg aagcgaccat
    21061 ccaccagttt ggaggtgaag ataaagtcgc caacgtgggc gcgaatgttg ttgctgccaa
    21121 tctgtacgcg cagcggattg tcgccgccgt cgagcatacg catcagttca atcacgcctt
    21181 tacgcggcac gatcaccgaa tggcttggca aagattgacc aattggcatt gaacagactg
    21241 ccagacggtg gccgtcggtc gccacggtgc gcagttcttc gccttcggtt tcaaacagca
    21301 taccgtttaa gtaatagcga acgtcctgat gcgccataga aaactgggtc gcttcaatca
    21361 gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc gctctgccag tcatcgaggt
    21421 tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg gctacgcccg gagcgcacca
    21481 gcatccgttc accttccagc tgtacggcaa tttccgcgcc ttcaggcaga ccacggcaga
    21541 tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc tgaaccagcg
    21601 caacgcgtgc caccatttcc atctcgagat cagtaccggt cagcgacaac gtaccgtcag
    21661 caacctgtaa cagcagatta ccgagaatcg gtagcgtagg acgaccacct aacggcccgc
    21721 tcacctgttg tagcggtttt aataaatgct cacgttctac ggtaaatttc ataggtttac
    21781 gatgacaatg ttctgattaa atttgaaaaa tcttctttga tatcgtggct ctcttcacgc
    21841 aactgctcga tcttacggca ggcatgaagc accgtcgtgt ggtcacgacc accaaacgca
    21901 tcgccaatct ccggcagact gtggttagtc agctctttcg ccagcgccat cgccatctgg
    21961 cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac tttgatcttg
    22021 tagtactccg ccaccgtctt ctgaatattg tcgatggtga ccagtttttc ctgcaatgcc
    22081 agcaagtcgc gcagtgcctc acgcacgaag tcgatggtga tcgcccgtcc ggtaaagttg
    22141 gcgttagcaa taacgcggtt cagcgcccct tccagctcac gtacgttaga tcgtagacgc
    22201 ttggcgataa agaacgccac ttcgcctggc aaacgaatgt cattttcgtc ggcctttttc
    22261 atcaggatcg ccacgcgggt ttccagctct ggcggttcga tcgccacagt cagtccccaa
    22321 ccgaagcggg atttcaaacg atcctcaacg ccgttgatct ctttcggata gcgatccgag
    22381 gtgagaatga tctgttgatt accttccagc agggcgttga aggtgtggaa aaactcttcc
    22441 tgagatcgtt ctttattagc aaaaaactga atatcgtcga tcagcagtgc atctacggaa
    22501 cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca gggctttaac catgtcctga
    22561 acaaagcgct cggagtgcat ataaaccact ttggcattcg gcttgcgcgc cataatgccg
    22621 ttacccaccg catgcagcag gtgagtttta cccagacccg tgccgccata aaggaacaat
    22681 gggttatagg caccgccagg gttatccgcc acctggcgag ccgccgcgcg cgccagttgg
    22741 ttagatttac cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt agaacgatag
    22801 gtcggttctg ccggggccgg gacgttatcc caacctgagc gcgtagaagg cgcagcacgt
    22861 tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac tgccgcttgt
    22921 ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca gctgtggggc atccgctccg
    22981 cagaaactgg ttagcagtcc attgatatta ttaaggtact tgtcccgtac ccaatcgagg
    23041 acaaaacggt ttggcgcgta cagggccagc gtgttatcgc tcagttccgc ctgcaatggg
    23101 cgtatccaca tactgaattc tgtggctggt aactcatcct gcaatcgggc aagacactgc
    23161 tgccaaagcg aaagtgacac ggcggactcc actcgaacaa aagtcgataa tgactaaggc
    23221 tgaaacattc atgattgttg acgtacgtcg aaaagaccct gcttgagggt gacgcacgaa
    23281 ccgctgtctg cggttatatg cccgatcaag atcctgcaaa acgatcggga ccgcggatca
    23341 tagcctaaac tgcgcaagag atcttctgtt tctcacagat tttcccgatt tatccacagg
    23401 actttccaga cctcgctaag tgtaaacgat cctgccgcga ggcgggcacg atttacgccg
    23461 catattggaa aatttaatga ccatagacaa aaattggctt aatcgatcta ataaagatct
    23521 aggacgatcc ttgcgcttta cccatcagcc cgtataatcc tccacccggc gcgccatgct
    23581 ggttttcact ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa aagcgcggta
    23641 aataaggaaa gagaattgac tccggagtgt acaattatta caatccggcc tctttaatca
    23701 cccatggctt cggtgtccat cgtttcattt ttcggcggat atccaataaa gccattgaat
    23761 ttattcaagt ttaggtagaa atcgccatga aacgcacttt tcaaccgtct gtactgaagc
    23821 gcaaccgttc tcacggcttc cgtgctcgta tggctactaa aaatggtcgt caggttctgg
    23881 cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa agctaacccc
    23941 tgagtggtta agctcgcatt tcccagggag ttacgcttgt taactcccag tcaattcaca
    24001 ttcgtcttcc agcagccaca acgggctggc acgccgcaaa ttaccattct cggccgcctg
    24061 aattcgctgg ggcatccccg tatcggtctt acagtcgcca agaaaaacgt tcgacgcgcc
    24121 catgaacgca atcggattaa acgtctgacg cgtgaaagct tccgtctgcg ccaacatgaa
    24181 ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct cgataaccgt
    24241 gctctctcgg aagcgttgga aaaattatgg cgccgccact gtcgcctggc tcgcgggtcc
    24301 tgatagccct cattcgggtc tatcaacgcc tgattagtcc gctactcggg ccgcattgtc
    24361 gtttcactcc aacctgttca agctacggaa ttgaggcatt gcgcaggttt ggagtgataa
    24421 aaggcagttg gttgacggtg aaacgcgtat taaaatgcca ccctttacac cctggtggtg
    24481 acgatcccgt cccgcccgga ccatttgata ccagagaaca ctaacgatgg attcgcaacg
    24541 caatctttta gtcatcgctt tgctgttcgt gtctttcatg atctggcaag cctgggagca
    24601 ggataaaaac ccgcaacctc aggcccaaca gaccacgcag acaacgacca ccgcagcggg
    24661 tagcgccgcc gaccagggcg taccggccag tggccagggg aaactgatct cggttaagac
    24721 cgacgtgctt gatctgacca tcaacacccg tggtggtgat gttgagcaag ctctgctgcc
    24781 tgcttacccg aaagagctga actctaccca gccgttccag ctgctggaaa cttcaccgca
    24841 gtttatttat caggcacaga gcggtctgac cggtcgtgat ggcccggata acccggctaa
    24901 cggcccgcgt ccgctgtata acgttgaaaa agacgcttat gtgctggctg aaggtcaaaa
    24961 cgaactgcag gtgccgatga cgtataccga cgcggcaggc aacacgttta ccaaaacgtt
    25021 tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga acgctggcga
    25081 gaaaccgctg gaaatctcca cctttggtca gttgaagcaa tccatcactc tgccaccgca
    25141 tctcgatacc ggaagcagca acttcgcact gcacaccttc cgcggcgcgg cgtactccac
    25201 gcctgacgag aagtacgaga aatacaagtt cgataccatt gccgataacg aaaacctgaa
    25261 catctcttcg aaaggtggtt gggtggcaat gctgcaacag tatttcgcga cggcgtggat
    25321 cccgcataac gacggtacca acaacttcta taccgctaat ctgggtaacg gcatcgccgc
    25381 tatcggctat aaatctcagc cggtactggt tcagcctggt cagactggcg cgatgaacag
    25441 caccctgtgg gttggcccgg aaatccagga caaaatggca gctgttgctc cgcacctgga
    25501 tctgaccgtt gattacggtt ggttgtggtt catctctcag ccgctgttca aactgctgaa
    25561 atggatccat agctttgtgg gtaactgggg cttctccatt atcatcatca cctttatcgt
    25621 tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc tccatggcga agatgcgtat
    25681 gctgcagccg aagattcagg caatgcgtga gcgtctgggc gatgacaaac agcgtatcag
    25741 ccaggaaatg atggcgctgt acaaagctga gaaggttaac ccgctgggcg gctgcttccc
    25801 gctgctgatc cagatgccaa tcttcctggc gttgtactac atgctgatgg gttccgttga
    25861 actgcgtcag gcaccgtttg cactgtggat ccacgacctg tcggcacagg acccgtacta
    25921 catcctgccg atcctgatgg gcgtaacgat gttcttcatt cagaagatgt cgccgaccac
    25981 agtgaccgac ccgatgcagc agaagatcat gacctttatg ccggtcatct tcaccgtgtt
    26041 cttcctgtgg ttcccgtcag gtctggtgct gtactatatc gtcagcaacc tggtaaccat
    26101 tattcagcag cagctgattt accgtggtct ggaaaaacgt ggcctgcata gccgcgagaa
    26161 gaaaaaatcc tgattcggtg agttttcgct aaaataaggg cggtcagttg accgcctttt
    26221 ttcttttcgt agggtggata agcaccgcgc atccgccaca caaagcaaca ggaacatcat
    26281 gagcgataat gacactatcg tagcccaggc cacgcctccg ggacgtggcg gcgttggcat
    26341 cctgcgcatc tccggcctaa aagcccgtga agttgccgaa accgtgctgg gtaaactgcc
    26401 taagccgcgc tacgccgatt atcttccgtt taaagacgcc gacggcagcg tgctcgatca
    26461 ggggattgcg ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg tgctggaact
    26521 gcagggtcat ggcggtccgg tgatcctcga cctgctgtta aaacgcattc tgaccattcc
    26581 cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta acgataaact
    26641 cgacttagcc caggccgagg cgattgccga tcttatcgac gccagttcgg aacaggcggc
    26701 ccgttcggca cttaactcgc tgcaaggcgc attctccgca cgggtcaacc atctggtgga
    26761 agccctcacc cacttgcgca tttacgtcga agcggcaatt gatttccctg atgaagagat
    26821 cgatttcctc tccgacggca aaattgaagc ccagcttaat gacgtgattg ccgatctcga
    26881 tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc gaagggatga aggtggtgat
    26941 tgccggacgt cctaacgccg gtaaatcgag cctgttaaac gcgctggcgg gccgtgaagc
    27001 agcaatcgta actgatatcg ccggaaccac acgtgacgtg ctgcgtgagc atatccacat
    27061 tgacggaatg ccgctgcata tcatcgatac cgctgggcta cgtgaagcca gtgacgaagt
    27121 ggaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc gcgtgctgtt
    27181 tatggtcgat ggcaccacaa cagacgccgt ggatccggca gagatctggc cggaatttat
    27241 cgcccgtctg ccagcgaaac taccgatcac cgtggtgcgc aataaagccg atatcaccgg
    27301 cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct cggcaaggac
    27361 aggtgaaggc gtggaggtgc tgcgtaacca tctcaaacag agcatgggct ttgacaccaa
    27421 catggaaggc ggcttcctgg cgcgtcgtcg ccacctacag gcgctggaac aggcagcgga
    27481 gcatttgcag caaggtaaag cgcaactgtt aggtgcctgg gcgggtgaac tgctggcgga
    27541 agagttgcgc ctggcacagc agaacttaag cgaaatcacc ggggaattta cctctgatga
    27601 tttgctgggg cggattttct ccagcttctg tattggtaag taaccgcgct tacgaagccg
    27661 tattctgact gtcagatgcg gcttcgcttc attgttagca ctcctgttat tcctcaaccc
    27721 tttttttaaa cattaaaatt cttacgtaat ttataatctt taaaaaaagc atttaatatt
    27781 gctccc
//
LOCUS       DAZOXR010000042        27102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZOXR010000042 DAZOXR010000000
VERSION     DAZOXR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.042"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..921
                     /locus_tag="ACHUNN_001513"
     CDS             <1..921
                     /locus_tag="ACHUNN_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0575920.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHUNN_001514"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHUNN_001514"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0575921.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHUNN_001515"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHUNN_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0575922.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="ACHUNN_001516"
     CDS             3882..4277
                     /locus_tag="ACHUNN_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0575923.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="ACHUNN_001517"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="ACHUNN_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0575924.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="dolP"
                     /locus_tag="ACHUNN_001518"
     CDS             4897..5472
                     /gene="dolP"
                     /locus_tag="ACHUNN_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0575925.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="ACHUNN_001519"
     CDS             complement(5586..6626)
                     /locus_tag="ACHUNN_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0575926.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="ACHUNN_001520"
     CDS             complement(6699..7334)
                     /locus_tag="ACHUNN_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0575927.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHUNN_001521"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHUNN_001521"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0575928.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="ACHUNN_001522"
     CDS             complement(7960..8403)
                     /locus_tag="ACHUNN_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0575929.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHUNN_001523"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHUNN_001523"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0575930.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="ACHUNN_001524"
     CDS             complement(8743..9246)
                     /locus_tag="ACHUNN_001524"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0575931.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="ACHUNN_001525"
     CDS             complement(9240..9764)
                     /locus_tag="ACHUNN_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0575932.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="ACHUNN_001526"
     CDS             9973..10968
                     /locus_tag="ACHUNN_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0575933.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="ACHUNN_001527"
     CDS             10977..11855
                     /locus_tag="ACHUNN_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0575934.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="ACHUNN_001528"
     CDS             12061..13068
                     /locus_tag="ACHUNN_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR0575935.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHUNN_001529"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHUNN_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0575936.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHUNN_001530"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHUNN_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0575937.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHUNN_001531"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHUNN_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0575938.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHUNN_001532"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHUNN_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0575939.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHUNN_001533"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHUNN_001533"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0575940.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHUNN_001534"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHUNN_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0575941.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHUNN_001535"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHUNN_001535"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0575942.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHUNN_001536"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHUNN_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0575943.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHUNN_001537"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHUNN_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0575944.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHUNN_001538"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHUNN_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0575945.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHUNN_001539"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHUNN_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0575946.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="ACHUNN_001540"
     tRNA            complement(26826..26902)
                     /locus_tag="ACHUNN_001540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6419 a         7516 c         6811 g         6356 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccaag
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatagcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc ct
//
LOCUS       DAZOXR010000043        26850 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZOXR010000043 DAZOXR010000000
VERSION     DAZOXR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.043"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHUNN_001541"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHUNN_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0575947.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHUNN_001542"
     CDS             567..650
                     /locus_tag="ACHUNN_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0575948.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHUNN_001543"
     CDS             750..1571
                     /locus_tag="ACHUNN_001543"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0575949.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUNN_001544"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUNN_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0575950.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUNN_001545"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUNN_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0575951.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUNN_001546"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUNN_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0575952.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUNN_001547"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUNN_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0575953.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUNN_001548"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUNN_001548"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0575954.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUNN_001549"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUNN_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0575955.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHUNN_001550"
     CDS             8347..9729
                     /locus_tag="ACHUNN_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0575956.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHUNN_001551"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHUNN_001551"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0575957.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHUNN_001552"
     CDS             complement(10485..10820)
                     /locus_tag="ACHUNN_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0575958.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUNN_001553"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUNN_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0575959.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUNN_001554"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUNN_001554"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0575960.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHUNN_001555"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHUNN_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0575961.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUNN_001556"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUNN_001556"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0575962.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHUNN_001557"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHUNN_001557"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0575963.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="ACHUNN_001558"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="ACHUNN_001558"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0575964.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="ACHUNN_001559"
     CDS             18642..20150
                     /locus_tag="ACHUNN_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0575965.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHUNN_001560"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHUNN_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0575966.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHUNN_001561"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHUNN_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR0575967.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHUNN_001562"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHUNN_001562"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0575968.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHUNN_001563"
     CDS             complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHUNN_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0575969.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHUNN_001564"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHUNN_001564"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0575970.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6738 a         6952 c         6458 g         6702 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aaacccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcatgg cgcatggcgc attttccgtc
//
LOCUS       DAZOXR010000044        26107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZOXR010000044 DAZOXR010000000
VERSION     DAZOXR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.044"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(30..1280)
                     /gene="icd"
                     /locus_tag="ACHUNN_001565"
     CDS             complement(30..1280)
                     /gene="icd"
                     /locus_tag="ACHUNN_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0575971.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1452..2105
                     /gene="rluE"
                     /locus_tag="ACHUNN_001566"
     CDS             1452..2105
                     /gene="rluE"
                     /locus_tag="ACHUNN_001566"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0575972.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2115..2576
                     /gene="nudJ"
                     /locus_tag="ACHUNN_001567"
     CDS             2115..2576
                     /gene="nudJ"
                     /locus_tag="ACHUNN_001567"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0575973.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2630..3736
                     /gene="mnmA"
                     /locus_tag="ACHUNN_001568"
                     /gene_synonym="trmU"
     CDS             2630..3736
                     /gene="mnmA"
                     /locus_tag="ACHUNN_001568"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0575974.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3772..4413
                     /gene="hflD"
                     /locus_tag="ACHUNN_001569"
     CDS             3772..4413
                     /gene="hflD"
                     /locus_tag="ACHUNN_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0575975.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4417..5787
                     /gene="purB"
                     /locus_tag="ACHUNN_001570"
     CDS             4417..5787
                     /gene="purB"
                     /locus_tag="ACHUNN_001570"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0575976.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5955..6626
                     /gene="phoP"
                     /locus_tag="ACHUNN_001571"
     CDS             5955..6626
                     /gene="phoP"
                     /locus_tag="ACHUNN_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0575977.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6626..8086
                     /gene="phoQ"
                     /locus_tag="ACHUNN_001572"
     CDS             6626..8086
                     /gene="phoQ"
                     /locus_tag="ACHUNN_001572"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0575978.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8162..9283
                     /gene="roxA"
                     /locus_tag="ACHUNN_001573"
                     /gene_synonym="ycfD"
     CDS             8162..9283
                     /gene="roxA"
                     /locus_tag="ACHUNN_001573"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0575979.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9332..10558)
                     /gene="pepT"
                     /locus_tag="ACHUNN_001574"
     CDS             complement(9332..10558)
                     /gene="pepT"
                     /locus_tag="ACHUNN_001574"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0575980.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10808..11944
                     /gene="potA"
                     /locus_tag="ACHUNN_001575"
     CDS             10808..11944
                     /gene="potA"
                     /locus_tag="ACHUNN_001575"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0575981.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11928..12785
                     /gene="potB"
                     /locus_tag="ACHUNN_001576"
     CDS             11928..12785
                     /gene="potB"
                     /locus_tag="ACHUNN_001576"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0575982.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12782..13576
                     /gene="potC"
                     /locus_tag="ACHUNN_001577"
     CDS             12782..13576
                     /gene="potC"
                     /locus_tag="ACHUNN_001577"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0575983.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13573..14619
                     /gene="potD"
                     /locus_tag="ACHUNN_001578"
     CDS             13573..14619
                     /gene="potD"
                     /locus_tag="ACHUNN_001578"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0575984.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14775..15596)
                     /gene="cobB"
                     /locus_tag="ACHUNN_001579"
     CDS             complement(14775..15596)
                     /gene="cobB"
                     /locus_tag="ACHUNN_001579"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0575985.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15612..16523)
                     /gene="nagK"
                     /locus_tag="ACHUNN_001580"
     CDS             complement(15612..16523)
                     /gene="nagK"
                     /locus_tag="ACHUNN_001580"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0575986.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16552..17796)
                     /gene="lolE"
                     /locus_tag="ACHUNN_001581"
     CDS             complement(16552..17796)
                     /gene="lolE"
                     /locus_tag="ACHUNN_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0575987.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17796..18497)
                     /gene="lolD"
                     /locus_tag="ACHUNN_001582"
     CDS             complement(17796..18497)
                     /gene="lolD"
                     /locus_tag="ACHUNN_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0575988.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18490..19689)
                     /gene="lolC"
                     /locus_tag="ACHUNN_001583"
     CDS             complement(18490..19689)
                     /gene="lolC"
                     /locus_tag="ACHUNN_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0575989.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            19951..21024
                     /locus_tag="ACHUNN_001584"
     CDS             19951..21024
                     /locus_tag="ACHUNN_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0575990.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21152..24598
                     /gene="mfd"
                     /locus_tag="ACHUNN_001585"
     CDS             21152..24598
                     /gene="mfd"
                     /locus_tag="ACHUNN_001585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0575991.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24742..25698
                     /gene="ldtC"
                     /locus_tag="ACHUNN_001586"
                     /pseudo
     CDS             24742..25698
                     /gene="ldtC"
                     /locus_tag="ACHUNN_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25781..26038)
                     /gene="bhsA"
                     /locus_tag="ACHUNN_001587"
                     /gene_synonym="ycfR"
     CDS             complement(25781..26038)
                     /gene="bhsA"
                     /locus_tag="ACHUNN_001587"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR0575992.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6327 a         6808 c         6646 g         6326 t
ORIGIN      
        1 cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc ttcaccaaac
       61 tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata agttacggtt
      121 ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc agtccacccc
      181 atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac tttgtcctga
      241 ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg
      301 tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc agaaatgtag
      361 tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag caggatctgt
      421 tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt gttcgggttt
      481 ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc acgcgccagc
      541 tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc
      601 agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg aaccagacgt
      661 ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg gaagcgaatt
      721 ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc ggcagagtct
      781 gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat aaccatatcg
      841 gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac cggacgcagg
      901 cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat accgccacca
      961 acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag atcaagggtt
     1021 tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt
     1081 tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc gtcgaccact
     1141 ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta agggataatc
     1201 ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga
     1261 acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc tcgtctgcca
     1321 ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat tttttgttaa
     1381 tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa gatgcgcgtt
     1441 attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa acttctttta
     1501 gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa cctgaaaacc
     1561 agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag ttcaccgatg
     1621 aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat gcagcaggtc
     1681 gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg ttgcaggcgc
     1741 gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg gaaggtattc
     1801 ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat ggccctaccc
     1861 tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg aatccaccaa
     1921 ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat gaaggacgta
     1981 atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga ctgattcgtt
     2041 atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga gaagcaacag
     2101 aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg cacgcagagg
     2161 gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac caacctgccg
     2221 ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta tgggaagaaa
     2281 ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt gcgccagata
     2341 aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc ccgacccagc
     2401 ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt ttaaaggcgt
     2461 caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt gggcaacgct
     2521 atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc atctaaaagg
     2581 ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac
     2641 cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct
     2701 gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg aagaagacga
     2761 cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg tctgcgacaa
     2821 gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca atgtcttcga
     2881 actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc tgtgcaacaa
     2941 agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg ccgattatat
     3001 cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc tgctgcgtgg
     3061 tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg agcagattgc
     3121 gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga
     3181 tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca ttggcgaacg
     3241 taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa tcattaccgt
     3301 cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg gtcagcgtaa
     3361 aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg tggtggacaa
     3421 agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc ggctgatgtc
     3481 tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca ccggcactat
     3541 gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg tcaaggcgct
     3601 ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga cgccgggcca
     3661 gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg agcagcgtct
     3721 gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa cgtggcaaag
     3781 aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa
     3841 caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact caacagtatt
     3901 attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc caacctgcgc
     3961 gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg cttaaacgcc
     4021 gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc ctcagcgaaa
     4081 ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct cgaacacttc
     4141 gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga tgtgatcagc
     4201 ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag cccacaagtg
     4261 caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct ctggcaccag
     4321 gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa
     4381 caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc actgaccgcc
     4441 gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc
     4501 gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca aaaactggcc
     4561 gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg ttaccttgat
     4621 gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat cgagcgtacc
     4681 actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc ggagatcccg
     4741 gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga tatcaataac
     4801 ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt
     4861 caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc gctgctgtct
     4921 cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc aaacgtcgcc
     4981 taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg caaaatcaac
     5041 ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga ctggcatcag
     5101 ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac tacccagatt
     5161 gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa caccattctg
     5221 atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa acagaaaacc
     5281 attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat cgacttcgag
     5341 aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc aagcaaactg
     5401 ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg
     5461 ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag caaactggaa
     5521 gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct ggctgagcca
     5581 atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct gaaagagctg
     5641 actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg tctggcgttg
     5701 ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat tggtcgcgcc
     5761 atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg atgctgtccg
     5821 gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct ccccgctggt
     5881 ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa taattaagac
     5941 agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc
     6001 ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat gccaaagaag
     6061 ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc ggattgccag
     6121 acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca ctgccgattc
     6181 tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt gctggtgctg
     6241 atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg caggcattaa
     6301 tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt caggttgatc
     6361 tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca
     6421 ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat tcgttaatgc
     6481 tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac
     6541 gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc gttcgcggcc
     6601 agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc
     6661 gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt cgcttgccta
     6721 cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt
     6781 acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata agttgcatgt
     6841 cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa
     6901 cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga tccagcctga
     6961 ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt
     7021 gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgatgacga
     7081 cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc ggatgccaaa
     7141 attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg
     7201 gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt
     7261 cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga
     7321 agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg
     7381 aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct
     7441 caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc
     7501 tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg agcaaatcag
     7561 ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt
     7621 gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct cagcgctgaa
     7681 caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt
     7741 tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata atgcctgtaa
     7801 atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt
     7861 ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg
     7921 tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat
     7981 caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat
     8041 ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccatactt
     8101 cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt ggatgcttaa
     8161 catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact ggcagaaacg
     8221 cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc cagacgagtt
     8281 ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc aggatggcaa
     8341 atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa ccaactggtc
     8401 attactggta caggcagtga accactggca tgagccgacc gccgcgctga tgcgaccgtt
     8461 ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg tacctggcgg
     8521 cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta ccggacgtcg
     8581 tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc cggatctgtt
     8641 acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg atattcttta
     8701 tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga actattccgt
     8761 gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt atgtgctgca
     8821 acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg ctcatcctgc
     8881 ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat tgatcaacca
     8941 gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc atgaactgga
     9001 tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc tgaaacaagg
     9061 tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg acgtgtatgc
     9121 caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg ccagcaacat
     9181 tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc tcgcgatgct
     9241 cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga tttaagccgg
     9301 atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt aactcggcaa
     9361 tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac tcatgcttac
     9421 catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata aacgacaact
     9481 gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg cagtcacgca
     9541 tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct cgcatattgt
     9601 agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct ttgcccactt
     9661 ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg tcgaaatcac
     9721 ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc aggtgataga
     9781 aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc gcatgaatac
     9841 gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga tgaacattgt
     9901 taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc agttcgccta
     9961 cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca tcaaaatgtt
    10021 tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca ccatgcggaa
    10081 tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct gctttgtcat
    10141 cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt agctgatgca
    10201 gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca atatcgccac
    10261 cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc ggtgaggtat
    10321 ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc ggtaacgtcg
    10381 ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct tcgagctgct
    10441 ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt ctcacccctg
    10501 cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt ttatccatgt
    10561 agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa atattgcgtc
    10621 aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat gaatataaag
    10681 ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc
    10741 cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg
    10801 cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt
    10861 gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct
    10921 gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac
    10981 cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa
    11041 cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta
    11101 cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa
    11161 aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga
    11221 aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat
    11281 tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct
    11341 ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg
    11401 tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt cagacaggat
    11461 tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa tttacgaaga
    11521 gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta acgccactgt
    11581 catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat
    11641 ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga
    11701 cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg
    11761 tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat
    11821 ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact ctctcgacca
    11881 aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa
    11941 gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc
    12001 tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca
    12061 aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc
    12121 tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctacc
    12181 cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc
    12241 tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa attttcctca
    12301 gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaattc
    12361 gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg ctaccgttta
    12421 tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc
    12481 gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca ctgacgatgc
    12541 cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtat
    12601 ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtccagttcc
    12661 tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg
    12721 gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag gtggaactcg
    12781 aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt acctgtatat
    12841 cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca tcaactggca
    12901 gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt tacaggcagc
    12961 gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta tcggttcact
    13021 gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta gcggaatgct
    13081 gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg tgctgtttat
    13141 gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca ccttctgcct
    13201 gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc ggatgctgga
    13261 agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca ttctgccact
    13321 ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga tggacgatgt
    13381 ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa aaatttattc
    13441 gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat tactggtgct
    13501 gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga aaggtaacac
    13561 aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc tctggcactg
    13621 ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa ctggaccgag
    13681 tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa ggctatctat
    13741 tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa agacggtgcc
    13801 tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga agggatgatc
    13861 cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga catgctcaac
    13921 aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc gacggcgatt
    13981 ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga tctgtggaag
    14041 ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt ccagatggcg
    14101 ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga agctgcatat
    14161 aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa cccggctaac
    14221 ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc tttcgttgca
    14281 cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat tttctggatg
    14341 gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa attgatcaac
    14401 ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta tccaacgcca
    14461 aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac gctctacccg
    14521 gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc cagcagcatt
    14581 tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg ggcggtggta
    14641 ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag taaagtttta
    14701 tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta gcgcatcagg
    14761 caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg caccacctgg
    14821 cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg ttcgagattc
    14881 agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc cgccggataa
    14941 acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc catataaatt
    15001 tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa gggtgccggg
    15061 aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc gagaacctga
    15121 ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac attggtatta
    15181 cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg atcgccgagg
    15241 gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc tggctgctgc
    15301 agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg atcgaaacct
    15361 tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc ggcgcgaaag
    15421 gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac tcttggtttt
    15481 tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa acgctcgcgc
    15541 aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga cagcatagca
    15601 acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc tcccgcatca
    15661 ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg acgaggcagc
    15721 ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc aatgacgacc
    15781 aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa atccagataa
    15841 cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat aatttcggga
    15901 gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg acctgataga
    15961 taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa atccagcccc
    16021 atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt aatgtagctt
    16081 ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt gccgagaatt
    16141 aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga aagggcaaaa
    16201 cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag gtcggcacgc
    16261 agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc ttctgtttcc
    16321 ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg gtcagcttcg
    16381 gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg tgtcggcacc
    16441 cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat ttttgttcca
    16501 ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc tttactggcc
    16561 gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc aacttgccaa
    16621 aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt gcaattccga
    16681 tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga tcagcttttc
    16741 aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa tcacaccaca
    16801 caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc gaattaaacc
    16861 atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt tcaccgccat
    16921 cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca tcgccagata
    16981 cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac caatccaact
    17041 tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct tattggcgtt
    17101 gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat caagatattg
    17161 ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac tcaactgcaa
    17221 aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct cgggattcga
    17281 gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat ccgccacgcc
    17341 tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat ccccctgaac
    17401 aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct tcacctgaat
    17461 tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg gcgcagcggc
    17521 ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag tccatggctg
    17581 atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat tcagttcgcg
    17641 ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga gggcaatgcc
    17701 aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc cgcgactaaa
    17761 acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat caggctcagt
    17821 tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt cgccagttgc
    17881 aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc cccaagcaac
    17941 tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc cgcaagtacc
    18001 agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc gccgccagaa
    18061 agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa catctccagc
    18121 gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat agccacgttt
    18181 tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc cagcttctgg
    18241 ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc gttaaagatc
    18301 acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt acttttaccg
    18361 gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa actgacattg
    18421 tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt gtcgcattgc
    18481 aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag cgcgccatga
    18541 agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa caatgacctg
    18601 taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga ttatcggcat
    18661 cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga tcgcaccgat
    18721 aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg gagttaagcc
    18781 ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc ctagtgaggt
    18841 aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca tcatattttt
    18901 ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct gccatttgct
    18961 gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat ccagccacaa
    19021 acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat cctcaatatt
    19081 caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa tcacattgaa
    19141 caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg gtaccatcac
    19201 gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc cgaggatgac
    19261 attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg gcgtaagtgg
    19321 atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc gcgcgctttg
    19381 cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca gtttgactgc
    19441 cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa ttgcctgtgg
    19501 catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca ctgacaatac
    19561 tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg agacgaaacg
    19621 accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata gagcgacagg
    19681 ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa gcgaaagcaa
    19741 gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag cccccgtaaa
    19801 tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt aatctgaaaa
    19861 atcatccaat acagacagat acccattaca cctcgggtca taacccgcag gatcaaccgc
    19921 atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg gattaaccag
    19981 atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac cttttatccg
    20041 catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg gatctatttc
    20101 aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg ctatttgatt
    20161 cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat ctggaacatc
    20221 ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc gctgaaccag
    20281 tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc cgattccacc
    20341 ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct gtatgcgtta
    20401 attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact attcgccttg
    20461 tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg aatgaacagt
    20521 gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac aataatgacc
    20581 tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct taccgttctg
    20641 gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc gattgtggtg
    20701 atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac cagcctgctg
    20761 aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct ggttgaaata
    20821 ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca agtcgaactg
    20881 acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc gggcttgctg
    20941 gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc ttcactgccc
    21001 gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca atttgctaaa
    21061 ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc catatgttga
    21121 ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt atacactgcc
    21181 cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct gtgcaacgct
    21241 ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac cagatatgca
    21301 aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg tgatgaatct
    21361 ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca ttatctcctc
    21421 gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga ttgttccggt
    21481 gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg cgctggtgat
    21541 gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca gcgccggtta
    21601 tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg cgttgctgga
    21661 tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg atgaaatcga
    21721 cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag aagcgatcaa
    21781 tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt tccgcagcca
    21841 gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc aagtgagtaa
    21901 aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg aaccactgcc
    21961 gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg atctggaaac
    22021 cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg gcgtcgatcc
    22081 gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc tcttctcaga
    22141 gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa aagccgcgaa
    22201 tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac aaaaagcgcc
    22261 gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt tctcggtaga
    22321 aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa ttgctccgca
    22381 acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga ttggcgctgc
    22441 cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa gcgatctgct
    22501 cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc ccgatacact
    22561 gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg agcacggtgt
    22621 cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg agtatttgat
    22681 gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc atctgattag
    22741 ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg gcgatgcgtg
    22801 gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg aattgctgga
    22861 tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc gtgagcagta
    22921 tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc aggccattaa
    22981 tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt gcggcgatgt
    23041 tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag ataaccacaa
    23101 gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg acaacttccg
    23161 cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc gcagcgccaa
    23221 agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc tgatcggtac
    23281 gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga ttgtcgatga
    23341 agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg cgaacgtgga
    23401 tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa tgagcggaat
    23461 gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta aaacctttgt
    23521 ccgtgagtat gacagcctag tggtccggga ggcgatcctg cgtgaaattt tgcgcggggg
    23581 gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg aacggctggc
    23641 agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg agcgcgaact
    23701 ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt gtacaaccat
    23761 tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac gcgcggatca
    23821 cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc atcatcaggc
    23881 atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac aaaaacgtct
    23941 tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa cgcacgatct
    24001 ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa tggaaaccat
    24061 cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga aagccggacg
    24121 cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc ggatgccgtc
    24181 gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct ataaacgtat
    24241 tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta tcgatcgctt
    24301 cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc gccagcaagc
    24361 gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga tcgaatttgc
    24421 cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac agccgcagca
    24481 ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg agcggaaaac
    24541 gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga tcgcttaatg
    24601 gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt tgcacttccc
    24661 tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt acaataacct
    24721 tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg gctaacgttt
    24781 tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc gctgccgcca
    24841 gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga cggtggttct
    24901 ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca ggctaacccc
    24961 ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt gcaaacccta
    25021 cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg tctctattac
    25081 tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca gttaggtggt
    25141 gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa cccaacctgg
    25201 acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt gcctacggta
    25261 gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc ggcctatggc
    25321 ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg ggtaagttct
    25381 ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt caccccaggc
    25441 accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa cggtgcgcgt
    25501 ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca gctgctgcca
    25561 attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga cgctgaagtg
    25621 atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg tcatcaagtg
    25681 agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc atgtcgcggg
    25741 gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa attactgctg
    25801 ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt gcgcccatct
    25861 catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc gcgttagcac
    25921 tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca gccgcaaagt
    25981 tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg tttttcataa
    26041 tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg ataactaaat
    26101 gatagat
//
LOCUS       DAZOXR010000045        25536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZOXR010000045 DAZOXR010000000
VERSION     DAZOXR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.045"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUNN_001588"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUNN_001588"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0575993.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUNN_001589"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUNN_001589"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0575994.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUNN_001590"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUNN_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0575995.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUNN_001591"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUNN_001591"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0575996.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="ACHUNN_001592"
     CDS             complement(5499..5696)
                     /locus_tag="ACHUNN_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0575997.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUNN_001593"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUNN_001593"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0575998.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUNN_001594"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUNN_001594"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0575999.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUNN_001595"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUNN_001595"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0576000.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUNN_001596"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUNN_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0576001.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUNN_001597"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUNN_001597"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0576002.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUNN_001598"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUNN_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0576003.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUNN_001599"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUNN_001599"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0576004.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUNN_001600"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUNN_001600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0576005.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUNN_001601"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUNN_001601"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0576006.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="ACHUNN_001602"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="ACHUNN_001602"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0576007.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="ACHUNN_001603"
     CDS             complement(17138..17191)
                     /locus_tag="ACHUNN_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576008.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="ACHUNN_001604"
     tRNA            complement(17425..17500)
                     /locus_tag="ACHUNN_001604"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17658..17733)
                     /locus_tag="ACHUNN_001605"
     tRNA            complement(17658..17733)
                     /locus_tag="ACHUNN_001605"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17698..17700),aa:Gly,seq:gcc)
     gene            complement(17944..18489)
                     /gene="orn"
                     /locus_tag="ACHUNN_001606"
     CDS             complement(17944..18489)
                     /gene="orn"
                     /locus_tag="ACHUNN_001606"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0576009.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18584..19636
                     /gene="rsgA"
                     /locus_tag="ACHUNN_001607"
     CDS             18584..19636
                     /gene="rsgA"
                     /locus_tag="ACHUNN_001607"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0576010.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19733..20701
                     /gene="asd"
                     /locus_tag="ACHUNN_001608"
     CDS             19733..20701
                     /gene="asd"
                     /locus_tag="ACHUNN_001608"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0576011.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20723..24046
                     /gene="mscM"
                     /locus_tag="ACHUNN_001609"
     CDS             20723..24046
                     /gene="mscM"
                     /locus_tag="ACHUNN_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0576012.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24197..>25536)
                     /gene="yjeM"
                     /locus_tag="ACHUNN_001610"
     CDS             complement(24197..>25536)
                     /gene="yjeM"
                     /locus_tag="ACHUNN_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0576013.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5911 a         7162 c         6470 g         5993 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttaatcttcg tctttcgaac
    17521 cgctactgcc ttggctgcct tcactcacct tagtcacata cttttgtaag ttcccaagga
    17581 ttcgttcagt tgccgccttg tcgcaattcg aaatactgcg attgggtggt ctgtgctttg
    17641 cagcactttt caaattttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc
    17701 aaggtcgtgc tctaccaact gagctattcc cgcgtactac ttaattttgc tttcgtaatt
    17761 ttcgcatttc tgcgtcgtta cgggaggcgc attatacgag aaatctttcc ctctgcaacc
    17821 ccccaaaaaa gttttttttg cgtttttatc tcaagtgctg aaattatcag caagacgttc
    17881 aatttagcgt caaaatcacg ccgccgcaag tggattccgg cgcttacgtg accaggaaaa
    17941 attttacagc ttgataaaat gctcgcggta gtaagccagc tccgccaccg attcacggat
    18001 atcatccatt gcctgatgcg tcccctgctt ggtaaaacca tccagaattt ccggcttcca
    18061 gcggcgcgcc agctctttca gggtgctgac atcgagataa cggtagtgga agtaggcttc
    18121 cagctccggc atgtatttaa acaggaaacg acggtcctgt ccgatgctgt taccgcaaat
    18181 cggcgatttt cccgcaggca cccactgttt taagaattcg agcgttgcca gttcagcttc
    18241 ccgatcgccc atcgtgctcg ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg
    18301 cacgttccag tcatccatca gcgccagctg ttcatcggac tgatgtactg caatggtcgg
    18361 cccttctgcc agaatattca gattggcatc ggtcaccagc gtggcaatct caataatgcg
    18421 atcgcgctcg ggatccagac cggtcatctc aagatcgatc caaatcaggt tgttttcatt
    18481 ggcactcatg ctattttcca cccattcaca gtgactatat tgatccacaa ttgcgtgtat
    18541 catagatgtt ttgcccatca ggggcgacca ggagtcagta cgattgagta aaaataaact
    18601 ctccaaaggc cagcagcgcc gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa
    18661 ggagaagccc gactacgacg acaatctgtt tggcgagcct gatgaaggta tcgtcatcag
    18721 ccgctttggt atgcacgctg atgtggaatc cgccgatggc gacgttcacc gctgcaatat
    18781 tcgccgtacc atccgttcgc tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc
    18841 ggcggcggaa ggcgtaaatg tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt
    18901 gttgacgcgt ccggatttct acgacggcgt aaaacctatt gccgccaaca tcgaccagat
    18961 tgtcattgtc tccgccattt tgccggagct gtcgctcaat attatcgacc gttacctagt
    19021 ggcctgcgaa accttgcaga ttgagccgat tattgtgctc aacaagatag acctgctgga
    19081 cgacgaaggc atggcgttcg tcaacgagca gatggatatc taccgcaata tcggttatcg
    19141 cgtattgatg gtttccagcc atactcagga tgggctaaaa ccgctggaag aggcgttgac
    19201 cgggcgcatc agcatttttg ccgggcagtc tggcgtcggc aaatccagcc tgctgaacgc
    19261 gttgctgggg ctgcaaaaag agatcctgac caacgatgtc tctgacaact cggggcttgg
    19321 tcagcacacc acgaccgccg ctcggctgta tcacttcccg cacggcggtg atgtgattga
    19381 ttcccctggt gtgcgtgagt tcggcctctg gcacctggag ccggaacaaa tcactcaggg
    19441 ctttgtcgaa ttccatgact atttaggtct gtgtaaatat cgcgattgca aacacgatac
    19501 cgatccgggc tgcgctatcc gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt
    19561 cgaaaactat caccgtattc tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc
    19621 tgatacggat gactgacaat taagctaacc gtcgttagaa tcgtcccctt tttttcagga
    19681 tccggcatgt aggccgaatc aggaacgaca aaacaatggc ctggaggcta ccttgttaaa
    19741 ttcatttaaa ctttcgctac agtacattct gccgaaacta tggcttactc gcctggcggg
    19801 ttggggcgca agcaagcggg caggatggtt gacaaaactg gttatcgatc tgttcgttaa
    19861 atactacaag gtcgacatga aagaggcgca aaagccggac accgccagct accgcacctt
    19921 taacgaattc tttgtccgtc cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa
    19981 tgtactggtc atgcctgccg atggcgttat cagccagttg ggtaaaatcg aagaagataa
    20041 aatcctgcaa gccaaaggcc acaactacag cctcgaagcc ctgctggcag gcaactatct
    20101 gatggcggac ctgttccgca acggtacgtt tgtgaccact tacctctccc cgcgtgacta
    20161 ccaccgcgta cacatgccgt gcaacggtat tctgcgtgag atgatctacg tgccgggcga
    20221 tctcttctcc gttaaccatc tcacggctca gaacgtgccg aatctgtttg cccgtaacga
    20281 acgcgtgatt tgccttttcg ataccgaatt tggcccaatg gcgcagattc tggtcggagc
    20341 gacgattgtt ggcagcattg agacggtctg ggcgggcacc attacgccgc cgcgcgaagg
    20401 tatcatcaag cgctggacct ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa
    20461 aggccaggaa atgggtcgct ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg
    20521 taaagtgaat ctggttgagc aactggaaag cctgtctgtc acgaaaattt gccagccgct
    20581 ggcagtatct accgaaacct ttgttacgcc agacgctgaa cctgccccgc ttcctgctga
    20641 agagatcgaa gcagaacacg acgccagccc attggttgac gacaaaaaag accaggtcta
    20701 atccatcaaa ggaaacgctg acgtgcgcct gattatcact tttctgatgg cctggtgcct
    20761 cagttggggg gcgtacgccg cgacggcccc cgatagcaaa caaatcactc aggaactgga
    20821 gcaggcaaaa gcggcgaaac ccgcacagcc ggaagtcgta gaggcgcttc agtctgcctt
    20881 aaatgcgctt gaggaacgaa aaggttccct tgagcgcatc aaacaatatc agcaagttat
    20941 cgataattat ccgaaactct ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacaa
    21001 gccgcgcagc gtgtcgccgg gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt
    21061 cagcagccag ttgctggata aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga
    21121 gattgccgat tcgctgaatc aactgccgca acagcaaacc gacgcccgcc gtcagttaaa
    21181 tgagatcgag cgccgcctgg gaacgcttac cggcaatact ccgctcaatc aggcacaaaa
    21241 tttcgcgttg cagtctgact ctgcacgtct taaggcgctc gttgatgaac tggagctggc
    21301 gcagctgtct gccaataacc gccaagaatt agcgcgctta cgctcagagc ttgcggaaaa
    21361 agagagccag caactggatg cgtatttgca ggccttacgt aatcagttga atagccaacg
    21421 tcagcttgag gcggagcggg cgctggaaag taccgaatta ctggcagaaa acagtgccga
    21481 tttgccgaaa gatatcgtcg cgcaattcaa aattaaccgc gaactatcgg cagcgctgaa
    21541 tcaacaggcg cagcggatgg atctcgttgc ctcgcaacag cgtcaggctg ccagccagac
    21601 gttacaggtc cggcaggcgt tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc
    21661 caatctgctc ggtgaagcgt tgcgagcgca ggtggcacgg ctaccagaaa tgccaaaacc
    21721 acaacagctt gataccgaaa tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct
    21781 gcttaataaa cagccgctgc tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc
    21841 cgagcaaaac cgtattctgg aagcacaact gcgcactcag cgtgagttgc tgaactcatt
    21901 gttgcagggt ggcgacacgc tactattgga actgaccaag ctgaaagtct ccaacgggca
    21961 actggaggat gcgctgaaag aggtgaacga agcaacgcac cgctatctgt tctggacctc
    22021 tgacgtgcgt ccgatgacca tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct
    22081 catttcgctg gacaccttca gtcagttggg caaagccagt gtgatgatgc tgaccagcaa
    22141 agagacaatt ttgccgctgt ttggcgcgtt gattctggtc ggttgcagta tttactcgcg
    22201 ccgctatttc acccgttttc ttgaacgttc ggcggcgaaa gttggcaaag tgactcagga
    22261 tcacttctgg ctgacgttgc gcactctttt ctggtcgatt ctcgtcgcgt caccgttacc
    22321 ggtgctgtgg atgacgctgg gttacggctt gcgcgaggcg tggccttatc cgctggcggt
    22381 cgcgattggc gatggtgtaa cggccaccgt gccgctgctg tgggtagtga tgatttgcgc
    22441 cacctttgcc cgcccgaacg gcttgtttat cgctcatttt ggctggccgc gcgaacgcgt
    22501 atctcgtggg atgcgctact acctgatgag catcgggctt attgtgccgc tgattatggc
    22561 gctgatgatg ttcgataacc tcgacgaccg tgaattctcc ggttcgctgg gaaggctttg
    22621 ctttatcctc atttgcggtg cgctggcggt ggtcaccctc agcctgaaaa aggccgggat
    22681 tccgctgtat ctcaacaaag aaggcagcgg cgacaacatt accaaccata tgctgtggaa
    22741 catgatgatt ggcgcgccgt tggttgccat tctggcgtcg gctgtgggtt atctggcaac
    22801 ggcacaggcg ctgttagcga ggcttgaaac ctcggttgcc atctggttcc tgctactggt
    22861 ggtttatcac gttatccgcc gctggatgct gatccagcgc cgcaggctgg cgtttgaccg
    22921 ggcgaagcat cgccgggcag agatgttagc gcaacgtgcg cgtggcgaag aggaagcgca
    22981 tcatcacagt agcccggaag gagcaattga agtcgatgaa agcgaagtcg atctcgatgc
    23041 catcagtgcg caatccttgc ggctggtgcg ctcaattttg atgttgatcg ccctgctttc
    23101 tgtcattgtg ctgtggtcag aaatccattc cgctttcggc ttcctcgaaa atatttcgct
    23161 gtgggatgtc acctccacgg tacagggcgt agaaagtctg gagccaatta ccctcggtgc
    23221 ggtgctgatt gccattctgg tgtttatcat caccacgcag ctggtgcgca acctgcccgc
    23281 gctgctggaa ctggcgattt tgcagcacct ggatttaacg ccgggtacgg gctacgccat
    23341 caccaccatc accaaatatc tgctgatgct gattggcggg ctggtcggct tctcgatgat
    23401 tggtattgag tggtcgaaat tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt
    23461 tggtttgcag gaaattttcg ccaactttat ctctggtctg attatcctgt tcgaaaaacc
    23521 gattcgcatt ggcgatacgg tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa
    23581 cacccgcgcc accaccatca gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc
    23641 gtttattacc gagcagttta tcaactggtc gctctctgac tcggtcacgc gcgtagtgtt
    23701 gacgattccg gcccctgctg atgccaacag tgaagaagtg acggaaatcc tgctcaccgc
    23761 agcgcgtcgc tgctcgctgg tgatcgacaa cccggcaccg gaagtcttcc tggtggatct
    23821 gcaacaaggg attcagattt tcgagctgcg tatttatgcc gctgagatgg gtcaccgtat
    23881 gccgctacgc catgagatcc accagctgat tctggctggc ttccatgccc acggtatcga
    23941 tatgccattc ccgcccttcc agatgcgtct ggaaagcctc aacggtaaac aaacggggag
    24001 aacgctgacg tctgcgggca aaggtcgtca ggcgggaagt ttgtaatgca tgaagctgcc
    24061 aaatgatgtc aacgtggctc atttagcagc ttaacatcat cttcagttat caccggccct
    24121 ggagagtatc atccatacgc attacttcct gacaactcat cgccggagag acgatgagtt
    24181 gcaggatttc atatgatcag tcgagagcta actcaggttt attcgccatg cgactgcaat
    24241 agttctggta aatcgccatc gctaacagcg agaagaagac agggccgcca atcatccaca
    24301 atgtgctgtc ccagtctccg gcttcaacca caggctgaat aatggtgaag acgttcgcaa
    24361 atgtcaccac cagtacgaca accaccatcg caatcattgc cgacatacgc gttttgaaaa
    24421 tcacaaatgg tctgtcgaga tcctgacgtg ctttaaagaa tgggaaagcc agcgcgagga
    24481 acaggtaagg aagcgtcata gacacgttcg ccatcagcgt cagcttgtta aagaacgcgg
    24541 atgcggtacc gccaccaaac gaaaccagca ggatgaagac agtaaccaac ccgcactgca
    24601 tccacatggc gatagacggc atccccatcg cattcaggcg cgtcatcggt tccggccaca
    24661 acgctttcgg cgtcccctgg atgatggctt tcagcggtga atagcaaagc gtaaagaacg
    24721 caccggtata agcgaggaac atcgaaagtc cggtaatacg cgcaaaccat acgcccagcg
    24781 acaatgacgc ttcaggtgac aaatgcagtg cgttacccag cgtcaccccg aggctcttca
    24841 tcagcacata ggtaatattg ccaaggttaa cggaaccatt acttaatacc tgctgccagt
    24901 ttgtgctgac gccccataaa aatattgcca gcgaataacc gattgaaata acaatagcgg
    24961 caaaaacaat acctttggca aagttctttt ctggattttc cgttttatcg accagaccac
    25021 caaccgcttc aattccgcca taggcaaaaa tagcaaatac cacaaacgat agcattgcca
    25081 gaccggactg ataacccggg ttcggtgatg caaggaaatt aatatcctgc gcgaaatgcc
    25141 caccatttaa taacaaaata gtaatgctta ctaacagcaa tactaaattc agacacatta
    25201 ctgcaatacc gcccaccgca gtaatgcggg caattttatt aatccccttt gaagcaacga
    25261 cggtgaccag aatcatccat gctactgcca gcagaccaac cacctgcgta ggctccagtc
    25321 cggcaatacg ccagtgctgg gtcatgtcgc taccatagag gaatgttgag aacggtaccc
    25381 aaactttcgc ggaggtactc accatccaga tgatataaga ggaaaaccac ataaacgtac
    25441 caatgaaggc aaaacgtggt ccgacactat tattcatcca ggaatagata ccaccttctt
    25501 ctttgcgata agcagctccc atttcagcca tcatta
//
LOCUS       DAZOXR010000046        25503 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZOXR010000046 DAZOXR010000000
VERSION     DAZOXR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.046"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(14..2182)
                     /locus_tag="ACHUNN_001611"
     CDS             complement(14..2182)
                     /locus_tag="ACHUNN_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR0576014.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            complement(2279..2863)
                     /gene="traX"
                     /locus_tag="ACHUNN_001612"
     CDS             complement(2279..2863)
                     /gene="traX"
                     /locus_tag="ACHUNN_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HFR0576015.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            complement(2892..4094)
                     /gene="traW"
                     /locus_tag="ACHUNN_001613"
     CDS             complement(2892..4094)
                     /gene="traW"
                     /locus_tag="ACHUNN_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HFR0576016.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSLRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            complement(4061..4675)
                     /gene="traV"
                     /locus_tag="ACHUNN_001614"
     CDS             complement(4061..4675)
                     /gene="traV"
                     /locus_tag="ACHUNN_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HFR0576017.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            complement(4675..7719)
                     /gene="traU"
                     /locus_tag="ACHUNN_001615"
     CDS             complement(4675..7719)
                     /gene="traU"
                     /locus_tag="ACHUNN_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HFR0576018.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGSGSLESLITSMSDSLNTAY
                     KNSGHKISCVFERDPEMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERYSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICARNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPQERKDEAVGIILSND
                     PDYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            complement(7809..8609)
                     /gene="traT"
                     /locus_tag="ACHUNN_001616"
     CDS             complement(7809..8609)
                     /gene="traT"
                     /locus_tag="ACHUNN_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HFR0576019.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEALLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISNTRVRLNKATEHLVKLIRRGEPVATGKKEKYNTAVKAA
                     ERALEHARLSARRLSLRHVQKAEITSTEPLSGKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            complement(8593..8781)
                     /gene="traS"
                     /locus_tag="ACHUNN_001617"
     CDS             complement(8593..8781)
                     /gene="traS"
                     /locus_tag="ACHUNN_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HFR0576020.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKCYTSTEPAHATQH"
     gene            complement(8845..9249)
                     /gene="traR"
                     /locus_tag="ACHUNN_001618"
     CDS             complement(8845..9249)
                     /gene="traR"
                     /locus_tag="ACHUNN_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HFR0576021.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKYLLLIPMMLLPKA
                     VLADGDLADMVRNVGEGAKTAQSSSITIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            complement(9300..9827)
                     /gene="traQ"
                     /locus_tag="ACHUNN_001619"
     CDS             complement(9300..9827)
                     /gene="traQ"
                     /locus_tag="ACHUNN_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR0576022.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            complement(9827..10531)
                     /gene="traP"
                     /locus_tag="ACHUNN_001620"
     CDS             complement(9827..10531)
                     /gene="traP"
                     /locus_tag="ACHUNN_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HFR0576023.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSRLNGIMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            complement(10531..11820)
                     /gene="traO"
                     /locus_tag="ACHUNN_001621"
     CDS             complement(10531..11820)
                     /gene="traO"
                     /locus_tag="ACHUNN_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR0576024.1"
                     /translation="MSAEQDAGKSGKKLAALLGLSSIILFGGSYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAYRQASGTPEEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATT
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQAEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            complement(11823..12806)
                     /gene="traN"
                     /locus_tag="ACHUNN_001622"
     CDS             complement(11823..12806)
                     /gene="traN"
                     /locus_tag="ACHUNN_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HFR0576025.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAILRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            complement(12817..13509)
                     /locus_tag="ACHUNN_001623"
     CDS             complement(12817..13509)
                     /locus_tag="ACHUNN_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR0576026.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            complement(13506..13853)
                     /locus_tag="ACHUNN_001624"
     CDS             complement(13506..13853)
                     /locus_tag="ACHUNN_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0576027.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            complement(13871..17638)
                     /locus_tag="ACHUNN_001625"
     CDS             complement(13871..17638)
                     /locus_tag="ACHUNN_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR0576028.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADDSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGTVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STASAPSPAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            complement(17728..18279)
                     /locus_tag="ACHUNN_001626"
     CDS             complement(17728..18279)
                     /locus_tag="ACHUNN_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFR0576029.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWKP"
     gene            complement(18294..18584)
                     /locus_tag="ACHUNN_001627"
     CDS             complement(18294..18584)
                     /locus_tag="ACHUNN_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0576030.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            complement(18581..19729)
                     /gene="traJ"
                     /locus_tag="ACHUNN_001628"
     CDS             complement(18581..19729)
                     /gene="traJ"
                     /locus_tag="ACHUNN_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR0576031.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            complement(19726..20544)
                     /gene="traI"
                     /locus_tag="ACHUNN_001629"
     CDS             complement(19726..20544)
                     /gene="traI"
                     /locus_tag="ACHUNN_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HFR0576032.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            complement(20541..20999)
                     /gene="traH"
                     /locus_tag="ACHUNN_001630"
     CDS             complement(20541..20999)
                     /gene="traH"
                     /locus_tag="ACHUNN_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HFR0576033.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            complement(21393..21977)
                     /locus_tag="ACHUNN_001631"
     CDS             complement(21393..21977)
                     /locus_tag="ACHUNN_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR0576034.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            complement(22037..23239)
                     /locus_tag="ACHUNN_001632"
     CDS             complement(22037..23239)
                     /locus_tag="ACHUNN_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR0576035.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            complement(23325..24149)
                     /gene="traE"
                     /locus_tag="ACHUNN_001633"
     CDS             complement(23325..24149)
                     /gene="traE"
                     /locus_tag="ACHUNN_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR0576036.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAENLITGQILPVSLFNTSLLPW"
     gene            complement(24300..25454)
                     /locus_tag="ACHUNN_001634"
     CDS             complement(24300..25454)
                     /locus_tag="ACHUNN_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0576037.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
BASE COUNT         5844 a         6232 c         6544 g         6883 t
ORIGIN      
        1 tgttttatta aaattatcct tttattccat ctttattatc atcctttgtg acatttttta
       61 gtctgtcctc tctaactcca gggctgtgtc taatatttct attagaacct gcaaaaatat
      121 cttttactga tgaggtgagg ttgccaagag tatttacacc atctctacca cccaagaaat
      181 tgataacatg gtcaggaaga tatgcttgta aagcaaatat tgcagctaca gacgttgtac
      241 acatacgggc gtaaattagc aggaacccgg caaggctgaa tatccctgtt aatgatttta
      301 cctgtacgtt aactagtgct gcgccaaaca gtgagtttaa tattgtgccg acggcaatga
      361 tggcgacaga ggcaaaaaag aaaccgaata ccatgagagg tggtcttatc atgctgtcaa
      421 ttaagtatat atatccgtaa gctgctcgac ttcctctgtc ctctgatgtt cctagatgtg
      481 ttgctgccca cagaggcccg gcggtgcatc ctattaatac gcttactatc cagttcccaa
      541 tgcctgtcat ccagaaaatg aatggtataa atggtaggta aattgataag gaaaaacccg
      601 cacaaaacaa caggaacaac aagaaatata ttgggggagc tgcagcatcc agcagagcaa
      661 caaatccttt aggtaatcca gagaacaaat taacagcctt tccgaaaata gagttatcac
      721 ccattgtcgc gagaactctg acagttgtat atcctgtcca gagaatttct gttgtaccca
      781 atgtataatc gccgatgttt ttcatcttaa ttaatgggtt aacttggttg ctggttgttc
      841 ctgttcctga ccattcagta gcagcttggt ttgtccattt taatatggtt ggcgctatac
      901 ttttcatgac cacactttgt gggtctgtgc tattagctgc attactttca tcttgagttg
      961 ttatcgttcc tatcgttgga gtatatgtgg agttctgtaa ttgagtcttg tatgccccca
     1021 taacagcact aaataaatca gtatcgccaa cttccccaag tgatgacatt gaagttacag
     1081 ctggagctct gtcagccagc tctgccagac gctggttagc agtggcaaag gtctgatacc
     1141 acgcgccaag cgtcacccat ccataggttg tgagataaga cttgagagct tctttcctgg
     1201 attgttctcc gttatcaata ggtagcgatt tttgtacagc gcgttcatac tcatcggctg
     1261 ctctctgaat ccgtgtctcg atatccggca atgttccgtt gccagaattt cttttttcca
     1321 gaaatgttgt cacaaactca ctggcagcgt tgtccatgtc gctaatcata ttatccatgg
     1381 ctgcacgttg tgctgagata acaccgctgt attcgttttt actgaaggga ttaaagaatt
     1441 ttccaatcgt cgattgatct gttgttccgt ttccttctac tgacaggctg gctgaaccac
     1501 agataccgct gccattggat accgtgacag tgtaattacc actggcggtt ttagctgatt
     1561 ctgtcatcag tgaagtcgag gatttgaccg tctgattaaa atcatttagt cctgcgttaa
     1621 ccgcatactt acacagttcc atttcaaaaa tgccacgcgc tgctgttcgt gtggatgcct
     1681 gaactggctg tacagtcatt gaataaccat ttgcgatatt atcagcggca agttgcacca
     1741 tcacattggc ggacccaacc cccattatcg atgcgcccca taacataatg agctgagcga
     1801 tactccagcc atttccggtt ggaacgatca tcagaaaacc tgcaacgaca ctcagtgtgc
     1861 cgacgatatc gcgtccggtg ctgaatacct gtccctgatg ccccgaacgc acaacatgac
     1921 ggataccaat gaacataaac cagacaacgg caagtacggc gatgatactg ttgaacgccc
     1981 cgaacatgct tccgatcaga gtgggggcgc tggttgacag tggattggtg actacatcac
     2041 cgaatatcgt gaccagcgcc tggcgagaca gatcgtcagg attggttgcg gcagacacaa
     2101 tatcctgata ggtcactgaa gccattgccg gcagtgagga tatagcaagg cctgcacaga
     2161 gtgcgcgaag taaaattttc acagtaactc cggaatagaa ttgcgtatgg gcaaaggcca
     2221 cacaggattt aattaagggg gatggcgaca tgccagtggt actggcatct ggacgtgatc
     2281 agtattgttt actggttacg gcaattaatg tggcattgcg ccagccattg cggtcgttca
     2341 gaaaatcacg gaaagtcccc tgctcgggtt cgctgacttt tcgttcatgc aactgccaga
     2401 ggcggtatgt aacaatcagt gctctggaag cacaaagagc gacgcctgac agtatcaaca
     2461 caagagtgct gatggcgcgt accagagtcg taagaggtaa tgaagaggcg gcaaagagca
     2521 taccactgag aaacaaagcg atggccagca gactccagca acagaatcgc catactgttc
     2581 tgcgccggcg gaaacgacga tccagatcgt ctactgacat atggctggcc tgcagagctt
     2641 cttcccacgt gaggtggagt tgcgcctttt tattatttac ctgacgaaat ctgtccagcc
     2701 tgcgtttttg ttgttccagc atatgctgta cagaattcgt tatcaggcga gtctccatca
     2761 gaggaacaaa aatgttaata atcccccggg tggctatgcg ggttatggat ggtgattttt
     2821 tgacgttttt cgtctggtct gaattttccg gtaacttatt cattttttca gtcctgttct
     2881 gacgtcagct ttcaacgtgc gtttgcctga ttgattgttt tctcaaggct accgagttgt
     2941 ttttcataat actggcgggc ggtcagagcc agttgctgtc cagagatgac attaccctgg
     3001 cggatctgtt ctttcaggtc gttcagttgc cagttcattt gcgcagtgat tcttaccagt
     3061 tcacggagca ggttatctcc ctgcatttcc tgtaaatcca ccaggtatgc tgtgttggcg
     3121 tagcgccggc cagcctcaaa agcttcaaac tcacgggtac tcatatatcc ccgtgctttt
     3181 gcttctggtg aagccacctg gtcgaagtaa gcggccgctg agtctgactg cagtgtttca
     3241 gccagtgctt tacgtgtgct ttcattaggc gtgctgtcag caatcagcgt cagttgggga
     3301 ttcgacgcag aatcaatgat gccgttgtat tcgttctgga gtccgacgta ggttcgccca
     3361 ctctgggtat tcacttctcc tttacccaga gcacgaccgg cagaggggcg ggaagtgttt
     3421 ttcatgtaag cagtcgctgc atctatttgc ttctgatccc atgtcagtgc cgcaggtgtg
     3481 tccgctgtac ctgcgccgtg gtaaatcgag cgtacctgac tgtctccacc tggtatttcg
     3541 cccacagaag gacaaacggc ggttcctcca aaacgtgcat attctgcttc tgtacagtaa
     3601 ctggcgtgta tggccgcgcc atcataagct tcccgcacgg gagagtttgc tgcgctggcc
     3661 agacggtcgc ggaggctacg gttgctcaca ccgccacctt tactgagttt tgaggctgcg
     3721 gatgctgagg cacttttgct ttctgttgca atccctgatg cggattcact gcagatggag
     3781 tccggcacgg tgaagctttt ccgagcctcc tcgaggcgct gagtttcatt actgaaaatc
     3841 atctgctgac gtgtcgcctg tccgttctgg gcaatgacac ttgccagctt ttcagagttc
     3901 tgattgatag cggtacccgt ggctgtctgg gtactcagta tgttgccaag gacatctttg
     3961 atggcttcca ttctggggat gacctgctct tcaacaggcc ggctggcgac gacggtgact
     4021 gaataagcat gagcctggca tgccgttcca ctcagggtgg ctataagggc tgcgagtaat
     4081 gtttttcttt gcattttatt gtctcctgct cgggatggag cacaaggtca ggaaggctgt
     4141 cgtattttct gtcttcaggt agtggggcca gtgggaaaca ctgatatcgc ctggtgatat
     4201 aacctttcct gttccggtta tcgatgatgc tgaaagaacg acgtctgacg cgccgtaagg
     4261 tggaagggtc agtatccata acaggccagt caccggcact cagtgccggc gttccgggaa
     4321 cggatggcca taaccaggta tcatcggaca tcagcgaagc cagcggtgaa gggagtatcc
     4381 actggatgac atgctgaacg tcgaatggtg cttcatccgg gacagaaacc tgccatattg
     4441 ccgttgttat cagctcatct gtgctggacg gcaacaacag ccgcatgcct gtgacctttc
     4501 cctgtgttct ggcatccgag gcgtatatca ctctgggatt gggggccgga tgtaacctga
     4561 agacacgtga cggcagagtg aatgagaaac tgtcaccttt tacctcaagt tttttaagaa
     4621 atacctgccc tctggcaggt ctccggcagc gtggggtgat atgaattgta cacatcagag
     4681 attgtatccc tgttttcgga tgagctccga ggccagttct tcaataacat tgtcactgtt
     4741 atgttggccc gcccggtgct caattaatga cgttgcagaa cctctgggga agttttcggc
     4801 cagaatctta cgtgcccgaa ctgaacctaa cttatttgtc agtgttttac gcagggcact
     4861 gtcttttggc gatgagttca gcgcccatag ctccagtgga ccgacggtaa atttgagtat
     4921 tctcgcaagc gtgccacttt ttaccctgaa tactcccaga acaggaacac cactaccatc
     4981 tggtgccggg ccttccggca ttttcaggaa gcgtttcagc ataaattcag gcacgttgaa
     5041 gttatccctc agtacgggga tatcttcggg tttgtagcgc agtaaccaga gagtatttgc
     5101 tgacttaaga acgctttccg ggtagtccct cagatactgc gtactcagaa ccgtgcggat
     5161 agcaaacttg cgttgctcac gctcctgggt atccagattt tcccagatga agtcaatgcc
     5221 ccgggcgtta tgcagttcat cataaacttt tgtcttaacc tcctgatcaa gttggttaat
     5281 acgtttaaga gcgatttcat ggtattcccg tggaagctgc tttaacactt catccctgta
     5341 ttgtggcagg gtgaagtcac caccggcgat ctgacctgca agcaggtaca tgatgccggt
     5401 tttaagcctg cctgcaggcg ttttgtcacc ggcaacatta ttcagatcga cagcgataac
     5461 ccgggtttcc gggttgatca taaaccgggt atacccggat atcatggggt agtcgttatg
     5521 tccctgctcc agggcgcggc ggatgtagtc cagcagaagc tcgtttgaac catcacgctg
     5581 aacagtaccg aaaacatccc tgagactggt atggcccagc atcgaggaca cttcgggaag
     5641 ctggggcatt gcctgataat gtgcccgctg agccgccatg atatacccgg cattgtggag
     5701 catatcccgg acttcaaacc aggttgatct ggcccaccag gctgcatcat gtttttcata
     5761 cagtcccgag tcctgcagtg ctgaatccac cagttcttcc gttgaggcgc gataaagtcg
     5821 cggattattt tcaccgtact ctttaaaagc cagctcgata agctggttga taatttgtcg
     5881 ggtgtctccg gggttacagg gttgaccggt accggtatcg acacagagag cacacagcac
     5941 cgatgacatg aaattctttt ccggcgtaat aggcttttta gcgccgtaca tcacgtcaaa
     6001 aaggttacgg gtgtagtccg gatcattgct cagaataata ccgacagctt cgtcttttct
     6061 ttcctgagga agactgtcgc gaataagctg aaccagtccc tgagcactgt atcccttatc
     6121 gatataggca atgaagggca ggtttttctg agcagatgat atctgtattt ccgacaacgt
     6181 attaatcaga actgatttac ccagtcccgg atcgcctggt gccagttctg tatgtttatt
     6241 ttgttgggat gacgccagac caacctcaaa tgcggaaccg tcttctgtat gtaacatcag
     6301 attccctttg cctcgccaga cagagcctgc cctgttaagc gggaaaagcg agatggcatg
     6361 tgacaacggg ggatacaggg gaaccgggcc ggagcctccg gatgccgcga gaatggtatt
     6421 cacccaggcg cgtcggggat cgccaaatgt tgtcgtggtg ccacatacgc cccagccttc
     6481 aatggctgat ttcagaatgg cctggtttcg tgcgcatatt tcacgtgttt ttccccacgt
     6541 ggaggccatg atggtcatta tgcagacagg ttctttttca tccgttgccg ccagggtcat
     6601 aacggattca tacattggac gaacggcaga aatgaagctg ctgtaagtca gaagcgtttt
     6661 cttcagatta agagccttca tcccgccggg catcagatcc attctgatgc gccaggggac
     6721 tgcgcggggg acggcacgga ccagttcgtt aaacgtctgc agattttgcg gaggtaaagt
     6781 aatcgatacc attccgtgcc agagtccgcc cgcctgaacc aggttgccct gtgtggtcac
     6841 ctgcgtgttg aataactgca aatgcaatga aggagcatgt agtacggatt catcgtcagt
     6901 ccagcgatat cctgctggct gggcatcttc aggcaggtgg ggttgccagt tacgcggtgt
     6961 gctgtaccgt tctgtctgac gacgtatttc acgcccgact tcgtgtatat ccagaagccg
     7021 gagaatcagg ccatcagatg aatgtctcag tgcctgttcc acattatcaa gaaaagcttc
     7081 gtggcggatt ttcagtgcag ataaggtcca ctgccagggg gactgggcaa agcgtgcttt
     7141 cggtacccgt tcagccagtc ttctgacaag ctcatcatgg gcagtgcgat cgctgttgct
     7201 gataaggtcg ggtccactcc agatcgccag ccagcaacgc tcccggacca gccatgggga
     7261 cagtgttgtg actttctcat cgaccacatc ctgtaactgg atacctgtat tagccagtga
     7321 gcgtttttgt ggggcgacca tgtcttcgat ttcctctttc cccatttcag ggtcacgctc
     7381 gaaaacgcag ctgattttat gaccgctgtt tttatatgcc gtattcaggc tgtcactcat
     7441 gctggtgatc agcgattcca gtgaacctga gccagtctgg tcggatgctt catccatttc
     7501 cctgaaagca ccgctgactt caaagacaga gagatattct ccacgctttg tggctgcaat
     7561 atagggcgaa ttcagccatg ggcgacgttc acgatcctgt ctgtccagac caattacagt
     7621 acgtaaatcg caatactcaa caaaactgct tgcgacagag aaacgggata gcgtgttcag
     7681 acagtcttcg atagtgaaga ttaaacggtt tatgttcata ggtgagggaa ctccggactt
     7741 acaggaggga ggtttgtgca gtttgatttt tttaatgcca ccccgcgctg gagtggcatg
     7801 aagaaaaatc aatcaacgta ttcttttttg atgaatgaat ctggtgtcag aatcagggca
     7861 ataccgttca ttttttcacg cagaggcgaa cgaccggaag gtgggtagcg acgaaaaacg
     7921 tcagcgaaat cggccgggcg gaaacttcgg aatttatcct caaccgccag tctgttttgc
     7981 atcagtgcag agagcgcttc ccgtcctcgc cggacccgga acttatcatt actgaaaact
     8041 tcctgtaatg aacgacggtt aagggccacg accgtggatg gatgaaggtc gggtagccac
     8101 accataacgc cctgtgctgc ggcataacca ttcagtgaat gccaggcgtg gcagaaagca
     8161 cataagctgt aaggcgggcc gtcttcatga aacagctcct gctctttccc tgacaacggc
     8221 tctgtactgg tgatttcagc tttctgtaca tggcggagtg aaagacgtct ggcagacaga
     8281 cgggcatgct caagcgcccg ttcagcggct tttactgccg tgttgtactt ttcctttttg
     8341 ccggttgcga ctggttcacc acgcctgatt aacttaacca gatgttctgt ggccttatta
     8401 agacgaactc gtgtgttact gattgtgtcg gtaagctgtc tgagtggcca tgagtcatcc
     8461 agtctgaaac ggtgcctgac gttttgccgg cagcagatac atcgaccggg cgttgttaat
     8521 tccggttccg gtaacagtgc ctcctgagta tgaaccacat caatttctgg aaagggatac
     8581 aaccacaagt ctttaatgct gtgttgcatg agcaggctcc gtactggtgt aacatttcac
     8641 ggcttccaga atgatgtttg cgatttcatc ggaacagcat tctgcaaaaa tgaattcctg
     8701 atcgtcccga ctgtcgtggg caatcacatt gccgttttct gttcgtcgta acgttatgtg
     8761 ctccgggctc caggcagaca tatgtgcctc cttgtattgc cgaataggaa gggcaccgga
     8821 taaatgccag tgccctggac aagattagcc aatggaaatt gaggaaatcc ccaactgttt
     8881 ctggctgcga ctcatcattt caggtccggc gacaagtacc gccccaatga cgatagatac
     8941 aatgcaacca gccagtccca tccccccttg tttcccaact tttttcaggc caatcaggcc
     9001 cccgagaaac agaattacgc cgatgaactg cgcaatagtg attgatgatg actgtgctgt
     9061 tttagcacct tcaccaacat tgcgaaccat atcggcaaga tctccatcgg caagaacggc
     9121 tttgggcagc aacatcatgg gaatgagcag caggtactta gtcagtttgc tccggcatga
     9181 cattacaaac agttgtgcat ggataaatgc ggcgtacaga cgggccgcca cgacacgaat
     9241 acgtgacata gtttctcctt aaatggatat atcagtggtt tatatgaagg tgaaaaaaat
     9301 cagaagacga ttccaatgct tgccagcagg gcattgagga cacgatcgat aaagaccagc
     9361 aggacgccgg caataaattt gacgtttccc ttactgacgg attcactggc actcagtgcg
     9421 gtatgtccat cgagtccggc cttgcgccag atggatatcc cgttcatgac aaatcccaca
     9481 ccggccagct tggccagcga cagaactgca tttgctgcgc cagcagcagc gccaaacgtc
     9541 tgaggttgta catatgcaat cgggccaaat gagtcagccc tgaatcccat ttgtacgcct
     9601 ccggcgttaa tcattgccgg gagtgatacg agcatgcagc tggttaacat ccccgcaata
     9661 aattttccgg gagacatttt taccggaccg gctgaacggg cgcgggcaaa cagggtaatc
     9721 atgctgatca ccccgatgaa ttctccccag gtaaacagga aatccattcc ggcactgaaa
     9781 atactgctgg caaagttctc aatggcggtc agggcatcca tgttcatcag cgtaacgtcc
     9841 ctgcactggt gatgatggtc cgggtagtcg gatcaatccg ctggatcacc acattgccca
     9901 gcgtctgacc ttctctgaca gcccaggtac taccctgcca gcgaatccag gccataccag
     9961 tttccatcga catgattttc attccgttaa gaacagacga cgtttttttc tttgctggat
    10021 gcggtgtgga agaggattca gtgacgcttt tctgtgtcat tattccgttc aggcggctcc
    10081 ggatatcatc cagctgacgg cgcgtttcct gtgcgtattc ttcatccttt tttatagctt
    10141 cgcccagctt gttaatgctg tcgtgaagca tattcagtgt tgcctgaatt ttttcatcgc
    10201 ggttatccag ctctgttttt acatcccggg caagctggct catatcttct cctgtccgga
    10261 cagtcatagt ctcagtggac tgaacagaag gtgatgattc ttgtgtggtt tgccaggtct
    10321 gtacgccagc atccgcatca ttaaacggag gcatgcctgt ttccgatggc gctctcagga
    10381 acagatacca tatggctgcg agcaacacga ctgcacatag cccccatgtt gctacagata
    10441 tcccccagac ggaacgtttc cagaaggggg cgggtttctc ttcgggttca ccgaagctgc
    10501 cggtttcatc tatttctgtt tcaggtttca ttatggtgtc tccggttgtt catcataccg
    10561 acgttgcatc acagcttttc tttgctccat ggctgtctgc attcgtgcct gcgagcgttg
    10621 ttccgcctgt gatgtacgat taactgaagg ggaaatgcca ctggaaaggg aagatgacct
    10681 ggcgtcgttt gtcattaccg ggtcaacaaa cagaattgat acgacttcat gctgatacac
    10741 ttctacctgt ttatagggct cactttctgc ctggcgggtt aatatctgac ttcctctttc
    10801 cgccatacca ccggcaataa cgcccgcaac agcttcacca ctgggcattc ccacgcgtcc
    10861 ggtaaccgtg ccatagttac cctgaataac ctgcgtgttg gcatctttat aaagtgtgcc
    10921 aatactgccc acacctccta acactgacgg cagaatgata tgtttaaacc agcgtgtatt
    10981 cacattgctg gccacggatg acatcaggtt gtcttcccgt tgagcatatg cattgacctt
    11041 aaggtccatg ccgttccagc tcatccggtc gaaatggatc tccacgccgt tacccaccag
    11101 tttgactccc ggactgaaca gcgttgcccc tgcccagggg ccagcgggaa tggttgcgat
    11161 aaccttggat gacgggttat cagaatccac tgcagtatca atatatgccg gtatacgggt
    11221 cagggcaggt atcagttgta tccctttttc tgctacaggc gtggacaggc tggcattctg
    11281 taatgcgaat tgctgcgtac cattctgccc tgttgtttca gccggagatt tattccacat
    11341 agtcgttgct atggttggtg tactaccggc aggatgctga tcttttattc ttacaatcag
    11401 cttttgcagc cgttccatac gcttttcact ggctgctcga tcctcctctg gtgtgccgga
    11461 agcctggcga taagcatagt tttccggttt tgctgcaggc tgttgttgct tttccggagt
    11521 gtccggaata tcaagcccct gcggaagact ggcaatgaac gtctgattat tgcgggctgc
    11581 cgccgcagcc cctcgttcat tatccgctct taacagttca cgatagtgag gcgtttcggt
    11641 aacgctgcgc gtaccgccac ttgcagcact gtttatgttc acggctgact gcatatcgct
    11701 gttactccct gatagtttgg aaaacgcgat atagctgccg ccaaataaga tgatgctgct
    11761 cagcccaagc agggcagcca gtttctttcc ggatttaccg gcatcctgtt cagcactcat
    11821 gattactcca gttctggtgt cacgtgaacg gactggccat tcacagagaa agtcagtagt
    11881 ggggtgacgg gaagcttcca gagatgagtt ccgtcggcag aggacaaggt ctgttcaaat
    11941 tcatcgcgca atatcgcccg ggaacgaacc agaaggtcat cgccgtgttg ccagatcgtt
    12001 gtatccggga cattacctgt gaatttcagc ctcttaactg aagggtcccg gggaggaata
    12061 ccatccagaa atgcctgtaa tgttgcgtca tgcagggcga ttttatccgt cgggatggag
    12121 acaaccgggc tggttggtcc ctgccgtggt attcgcagat ccagacgact gtccatttcc
    12181 tgactggatg acggcgtatc tgtttcgcca ctggttacgt tcaggatgac cggtaccgac
    12241 agacctttca ggtagacaga aatgtttccg gatgcccatg gccgcaatgg tgtgattgtc
    12301 accatgttct cgttgtactg cacatcaaag agttttggtg ccgcgttata aggaggatcg
    12361 gattgtggcc atggtgaacc gttgatatcg gtaaatgtca ctacgctcag gttgttcatg
    12421 gctgtgcgaa caagaggaag gctggcaccg ggggacagat taactgtcag agagctgata
    12481 cggggtacaa cactggtgat gggcgcattg atggcccgct cgttatccgc catgagactg
    12541 cgtagtgagc gaatctcatc ggcggagagc tcagaatcaa gtcttgccgc atcctgtacc
    12601 agcgggctgg cctgcccggg ggcaggcagc tcacctttta ccagtgttgc cggaacacct
    12661 ccggtgtttt gggaggcgtt ctgaacgtga tcgctggcag aatttgtcgt ttgtggtgtg
    12721 cgtgcgttct gccatcctgc gttatctgca gacgtggcgg cgctgcacac caggagcagt
    12781 gtgctcagta aataacgtga ttgcatatta cttatctcag gagttcggtc cggcgttacg
    12841 ggagatcatc tgccggattt ccataccgga tggtttcagt ctggggtcaa cacgctggat
    12901 ggtgatttcg aaaacaaaag actgttctgg cttgctggtt gtctggccga ccagacgcat
    12961 acggacagga tactgaaatg tccagaacca tacgccgtcg gacatttgtc cctgtcgtac
    13021 cagaacgcca gcgcctgtcg tggcggtcag attcatcttc tctttcttga tcgtctccag
    13081 aatgttagat gcctgtaatg cattcacata tccggcaaaa ccttcgtcag aaaaacgcgg
    13141 agacagagag gaaatctgtg aacgatagtg cacaaaatcc aggttgaatg cggtcgccag
    13201 tgcctggctg ccaaaggcca ttgcgtcatt ctgactccat gctggctgat ttgttggtgc
    13261 cagcctgacc agacgaccat tgtctgtagc gaaatattcg cgctccacat tcgttgagtg
    13321 ccagtactga atggcgttac cagtaatact gataagaaga catgtgcccg tacataaaag
    13381 cgcttttatc agcgcaggga cattatttgc ccgctgttct gactgcttca tagccctgat
    13441 tgtgctcttc agtatttccg ggtcaggtgc cggatgtgat ggcgaggatg gctcggtatt
    13501 ttcggtcata ggttgtagtc cttccaggga tcaatattcc ccggcagctt ttcattcacc
    13561 ttgtccacga ccgcgtcgca gacctggttt tccagctgcg acagaatgtc gtcaaggctg
    13621 gggaactggg ggaggtttat gctgattttt ttgattcgtg acagacagga ctgcagggat
    13681 gaggaaacat cgttttcacg ttgtgaccat gcatccgcag ctgcacgtgc tcgctcatat
    13741 cctgtattac taccggcctg tgccgcgcta cccgcacgac aggttgcagc acttgccgtg
    13801 gatgaaatca ggataaggcc gagcacggca accagagact tatgcatgtt catatggctt
    13861 ccttaccggc tcatcggccg gggctgtggg agagatcctg atgcagagtt ttttcctgcc
    13921 tgtcctccgg cttttggctg ttggaccact gctgccatga ctgctcgaag cccaggttcc
    13981 ggtacaacgc atcaggttgt ttttccggcg tgtagacagc agcaaaaagc ggttcttccc
    14041 gggttttcag atgaaactgc ataagctccg gtgcggggcg attcgtgctg tcagcatcgt
    14101 ggtttaccgc ttcaccataa cctgcaaagc gtaaaccttt gtcggtcagc gcattgataa
    14161 ccagctgact gttgtcggct gatggggtgg ccagtgccgg atacaatgtg ccgttcagcc
    14221 ggaccacacc ctgcagatgt tcatcggcac tctggacaag tagcaggttg agttcacgcg
    14281 tatccttatc cactgatccc agaacaggtg tcagcttgtc aggggctgcg ctggttattt
    14341 ctgttacctg atttgcgttc tgtggcgcgg agaccggatg tcggtcttca ccgataaaca
    14401 gatgtgagcc gacgttttct ggcatacgta acgctttcat ctcttcgggc cacccctggc
    14461 tgatttgcgc agccactttc ataaaggttt cgaccggaat cgcctgaata cgctgacctt
    14521 tatccggatt atcatgtgat accgagatcc cgttggtaac tgactccatg gaccacttat
    14581 tccggaaacg tgctttttgc tctcccggga ggatgaattg ttcgcggttc tgaagtgtca
    14641 gtgtcaccac gtcacctttc tgatggtttt tgaccagact ttccagttgt tccccccagt
    14701 agagtttgct gccactgcgg tttttaagct caacaaagaa actctctcct ttgccgggtg
    14761 catattcagc acgaccaaaa ttgtgcagta cggcagtcac gctctctccc gggcgaagtt
    14821 ttcctggtgg ttcttcgtct ggtgccacat ctgccgtttt gcctggttca gccggtgatg
    14881 gtgcagaggc ggtgctgacg gttgatgctt caggggcaac aggggacgcc ggggattgtg
    14941 tggcttcttt ttcctgaacg gggtcaggga cagccggttg ttctggggta ttccggttaa
    15001 gttccggtgt tggctgatgg gtgacaactg catccttact gacagccatt tccttgcgta
    15061 atttttctag ttcagcccgt tgatctggca atttcatccg gacatcgatg ttgtgctcga
    15121 taatcaactg cattgctttt tgcttaaagg catcactgcc cgtcagctct atgacgcccc
    15181 cataaaattt ggtggccaca gcaagtgcgg ccagaacgct gcgatcgtca ttgctggcac
    15241 cgttgaccat ttccagccgg tttacccggt caatgaaggc aggtttgcca tccagtttgt
    15301 acaggacggt tctgtctgct cgtttttccc agtcaagctc tttaagaatt tcgtccatgt
    15361 tgagcgatat cggtgcatca gaacgttccg gggcgtagac aatactgtca ggtatcggtg
    15421 gagtcgtcgt ggtttccgct gagggcgtgg gatcactgtt ctcatgtgcc tgtgaatccg
    15481 tttttttacg cgtaaaaaag ccacgcgctt tgtcgagaaa cgatgaagta ttatcagaag
    15541 gtgatgtgct ctgaacaggt tgctgtggag tgggttcatc ccggacagcc ggttctgcag
    15601 tatctgttgt ggcctgaagt tctgactcat ctgcctgaac gtggtctgtg tccggttgcg
    15661 acggcatatc actgttttcc tgcagatgct caggattttc attgttcatc agctcctgat
    15721 atgcgctgta gttttcgtat tcttcggggc catctgactc aggaaggaac ggctctggct
    15781 gaaggggaat gtcttcagtt acatggttga ccgcctcttc ccggggagta tccggggata
    15841 ttccggtgct ttgtttgtca tcaggctccg tcatcacggt catttcctct ttgactgcat
    15901 caggggaagg tgatggctcg gctgattcat tcatggtttc agatgaggga ctgtcttcag
    15961 gggatgcggg ggcggcagca ggggccggta tttcaggtga agtgccggat tcctgttgtg
    16021 actcgttttt ttccggttgt gacagttcgg cttcaaattc acgccagtct ttatctgtgg
    16081 ccaccgagac aactttttcc ggaggttccg ccggatgagc gggttcatca ggggcagatg
    16141 ccgttgtact ttccggggac gccggtgatt ctgcagggga gtcctgaact acagagcttt
    16201 ccgtttgcgc ctgaacgtta tcagcgtgcc gctcatcttt gccgttattt tcagtggcgt
    16261 gactggtaaa tgactgtgtt tcatcagcaa tgttactgct ggaagcagct ggttgttcct
    16321 gctgcgtggc ggacggcaga gaaggtccct ggtcatggct gtgccctgta tcgggtgatg
    16381 tttcaggata ttctgtcgga gtcatggttt tttcctcagt gtggaccggt gtaaaaacga
    16441 ggttgccgat caccagtgcc tcgcggattt ccggggtgtt actgttgggg gtggaaaccc
    16501 cgatgtgttc taactgggcg ttgatgacat gctgaaaagc ggcccgtccc cggcttgcgt
    16561 ccaggtcatt gaaatcggtc agcccctgcg ctttttcggc atcggtgaac gcaggaaaga
    16621 tgaccgttcc tcctgtcagt tctgcggctt ttgttgcact gacgatacct ttgttaaccc
    16681 ggattgcatg atcgttgtcg gcacagaaga taaaggggct ttgcgtccac ttttgccggg
    16741 cattttcggc aacggcaatc atgttgcctc catccaccgt catcagtacc ggaaggcccg
    16801 tggcttcgtg cagggaggcc gcagtggcat agccctcggc aaacagtacc ggctgaccgt
    16861 tgcgcggtgt gcccagcgca aaccagttac cggttttctc tgaatctttc aggatgcggg
    16921 catccttccc cccggtgacc ggaatacgct ggtaagagcg tatcgcgccg ttacggttac
    16981 tgaagggaat aaccagttca tttttgttgt ttacccgcac ccccggtgca gcctgaatgc
    17041 ctttccgggt caggtattca tgtgctgtgg cctgcggcca tttgttgaca tagcggcggg
    17101 catacgcggc ctgtcggttg tactgtgctt tcagttcccg ctccgcgtcc tccctgcgtt
    17161 gcattgaatg cgctttcagg tggagtcttg cgcggggatc tgtctgttct ccgccggaga
    17221 aggtccaggt gatcggcgaa tcatccgcgc tgcgataatc cctgtaccag ccggcaggtc
    17281 tgccgtccag gaatccgcga tatgcgccac ttttttgtcc ttttttgtca tcggcggtcg
    17341 ggacacggtg gattttcccg tccatgaccg gcagttcttt cagaaccagt ccggcattct
    17401 ccagaacctg ggcaaactcg gtgaccggat cactgccgtt catggagacg tcctgtggcc
    17461 ggggcagcca gcggtccagc ctggacaggt ccgcccccgg gcgggcgaac cagagtttgt
    17521 gctccttgtc ccagatgacg ggcgtgtgcc cgttcatcgg gggatgtgct tttttgagat
    17581 cttctatttc atctgcaggc acggcaagcc aggtgcggta gcgggataca gacggcatga
    17641 gtgcttctcc tgaaaaaaga cggaaaaaaa ccaccgccgg aagccggggg tggctggggg
    17701 gatagaaaat cgggggagaa atgtttgtca gggtttccag tctgtaccct tattccagcg
    17761 atcctgccag tgttcaagat attgtgcggc tacatccggc atattcttga gcacaacgac
    17821 attctctgaa ttcttgcgtg cagcagcacg agagaagtta taggagccag tttcaaccgt
    17881 gtttccgtca acaatgatga ctttgtcgtg atggatgggg aaggcatcta cagtccggag
    17941 aggaatgtcc agcaggttga tatatttcat cgcttcctga cttgcccggt tcgtattccc
    18001 cttgtcatca acaacaatac ggacgtccac tccgcgtttt ttcgcttttg ccagcgcatg
    18061 cattacatcc gggtcggtga aggaatatgc catcatcctg atggagcttt ccgcactgtt
    18121 gattgtgtca agcaccagag cggatgcgct tccttcagga gaaaagccga ccttcacgga
    18181 tggcgtgtcg atcattgcaa acgatggtgt cgtgaacagt aacgggcatg ccagtagcga
    18241 tacccggagc agagtaacga tagttttttt acctgacata aagactccag aatttaatcg
    18301 aggccagtcc agtctcgtgg tgattccgta tggtggcgat accaccatgg acgaccggag
    18361 gcgatggccc ctctcagaat gctgaaaatg cgtgtacaga tagtcctgac agtccagcct
    18421 gaacggtcga gaataacgaa aaataaaatg atgccggtgc aaatccagaa ggttgttttg
    18481 gagggaaagg ggcatataaa cagataaatc atggtgaacg gcagaggtac gccctggatg
    18541 ttgactggcg gtgttgcata tcgccagaaa ccttctcttt tcatgaccgt aactcccctg
    18601 gcgggataaa aagaatcgct tctcttcggt caacttcatt ccggatatac atttccagaa
    18661 tctggtcccg gatacggcgc ttctcctgcc ggattatggc atcaatgtga tgcccccact
    18721 ctgcatgcgg cattccggaa agattgtcgc gaagctcatc gttaatgacg atatattccc
    18781 ggacggcttt acgaccaccg tcatttgttc tgaccagaac ctgaaccaga atgaactgca
    18841 acagtgacag gacagcccag gccatcgaat ccctgatgac agggggaaac agcccaagaa
    18901 gacgtgccag cgtttcacca ggagatttcg tatgcatggt gctcaggcag taatgcccgg
    18961 tgttgcctgc ctgtaccgct gcgtcggcgg tttcgttatc cctgatctca ccaacaccga
    19021 taatttcggg attacgtcgt acagcagaac gcagtccggc agcaaaactg accacatcac
    19081 gcccgatttc tgcctgatgc ggtgggagca gatcgttttc atttcccagg atatattcca
    19141 ccgggtcttc atatgtcacg attttggcgt cggggaaatt gtccataatg taacggtaca
    19201 gtgctgaaca cagcgttgat tttcctgacc cggtttcacc acatacaaat cccagaccgc
    19261 ttttgcagac cattgcttca agcaggtctg gttcaatgtt catggagaga atgtccggta
    19321 tttctgtcgg gatgacacgc atggttattg agattgcttt ctcctccgca ccagatgtgc
    19381 cctgaatgag gtgagagcgt aaccggatgc gttctccgcg tttaagacca tagcgcccgg
    19441 atgcatcccc gttaatctgt atcgtcctgt caaccggatt gcctgccaga acgcggggaa
    19501 tgacttcccg accgaacagt tcatcaatca gggaggacat gagtgtggtt ggtaacgttg
    19561 cactggaaca acgcacccgt cggccaaagc gactgacaga tacaggtgac ccgccggtca
    19621 gatccacatc actgacttta tgtgcggcac accagacaaa gaaatgccgt agttcatctg
    19681 cagtgaaacg ctgaaacgga aaaagaccaa actcatcagg aatattcatc gggagcctcc
    19741 ttcagtgcct ataagcggac gccagtgaga tttctgaaga ttgaattccg catgattttt
    19801 aaggcgacga acacgatctc cagtgaccag ttttttcccg tcacctgtaa cggtttgcat
    19861 ttgatcagaa acatccggac gggtgatcat gccctgacgc cagagaagtg agtacagcat
    19921 catgccccgg taatcacggg tgagggtttt ctggttggct tcaagcgtca ggtcggcgtt
    19981 ttgtcttcct tcctgccatc cctgccgtaa tgcggtttcc cacacttttc gttgcacgct
    20041 gtcttctgga acgacgctgc cttctgtacc cggagtggct gttgttgaaa gcccccggag
    20101 aagccagtca cgccagcccg gtggattgct gacaaagcgt tcagggcgaa tgatggtcca
    20161 gacccgggaa gaggtccgga tttgatctgg cgtgatatga gctacgtcct gtgcttcatc
    20221 aataaccggt ggtagccagc cttccgggct aatcagtggc cggaaatcgt ataaggcatt
    20281 aagtgtgctt tcccgggcgt tgagcgcctg aatcagctcc catgaacgct gagccttgcc
    20341 accacgaaat ccaagcgttt tacctgcgtc tgtcagcatt ttccagcgtg tttctgacag
    20401 actatccggc ttttcacttc gtggtttaag ccatgcatta atgtcaggtg gtggcgtatt
    20461 gacaccacct ggcgagatgg taggggaaga agaacaacca gtaagcagta ttactggcaa
    20521 aaggagagag gtgagtgctt tcattttttc cggttctccg gctgcatgaa gctgacctgt
    20581 aacaagcccg gatattgatg gattgttgct ctccaggctg tctgggcttc aataagtctc
    20641 aacgtattgg aaaacgtcat atcccggacg tgaaggttga ctggtaacgg caggcggaca
    20701 ccattgtagt taaactgcat ccccctgact ctggccagtt cgttcagtaa ttcaatcgca
    20761 tcaccttccc agtcaaagga aaggcgatcg ctgttagctg tcagaagatg agaccccgtt
    20821 gaaaccggaa gttggccgga aaacacacct ttgtcccata atgtgtactg agtgtgccgg
    20881 gtaaccgcca catcagaggc agcaccgtag ttacgcggaa caggagtatg ctgagaaggg
    20941 gagttgcagc cagccagaaa aagggcggca agacaccccg caggtataat acggttcata
    21001 ataattcctg agcagttaaa tcagaatggg atataccggt gctccggtac agacaggatg
    21061 atgtttgttt ccggctatac ttgcgttacc ggaaataaat gccagtgcgg ccaggtaaat
    21121 agcctcatag tttactgaag gtgattattc ctgatactgg ctggttgatt ggtcgtcaga
    21181 cataatatga atgtgagcgt aatctttaac gctccgcgcc gaatcgatcc atgatggatg
    21241 actcgacgat ttatgaatcg aacgtaccgg cgcttgtccc tgaaacagtt cccggccctc
    21301 gtccggataa cctgcaattc agacaggcac atgtgcccgt acactcttgt atcttactga
    21361 gtatatcaga atttatgtaa attcaaatat ctctatgcct ctttgttgta ttttccgtca
    21421 agtatgagcc gggtaccatc gtaatgcatg atcagggcat caaggtacgc cggtttgaat
    21481 ttcttgccga gcctatctct ggccagaaat gataaacaat gattatgtgt cattattact
    21541 atgttccttt tatgagattc tcttgcgatt gattcaagcg tcttataaag atcattattg
    21601 cagtctgata aactgtccat aacgacgggt tctttaccgg agaagaattt tgcagtttgt
    21661 atggttctta ccgcattgct ggagtaaata tcatattcac tgaagattgt tgcgaactta
    21721 ataccttcct gctgtgcttt ctctgtaccg gtaatagtga ttccgctctt atccgaatag
    21781 cagggcatat ctgaacggtc gcaacgttct ccatggcgga aaaggaaaat aacggtgtta
    21841 tcagcattaa tttcttttgc ctgttccagg gtgatatcgg gaagcgagct ttttgctttt
    21901 gtcactgcag ctaccaggaa aacagaaaat aaaagaatga atgctgtctt tgaataagaa
    21961 gaacgtggtt tcatcatgtt atttaaactg aatataaaaa tacccccggg tttagtgccg
    22021 ggggcgggga ctgtaatcag aaagtgaact gaagttgtgc cattgcaccg taatcgtggt
    22081 cagttcctac caggccggaa acatcgatat gtacgacgtc aaatggagag ataccaacgc
    22141 cagcggtgaa ggcgcttccg gagtttgatg ccatgttctg acgataaccg gcacgcaatt
    22201 gcgcccattt ccatgcgtta aattcagcac caacacttgc aaactgacgt ttgctgtcag
    22261 aagtgaagcc actggcagga gtcagatcaa cgtcaagagc agtggtaaac aggtcattat
    22321 gccaggatac accggcggtt gcctgtggac gaactttgaa ggtttcttta aagccattga
    22381 cgactttagt atcaatactg cgtggaatga tgttctgggc aacaagaccg acagtccagt
    22441 tgtcattcag gtctgtataa gcaccaatat cggcgttgaa tccgtttttc gtgttgtggt
    22501 acttgtcacc gtcaaaatcg tctttgtcgt aatcacgaac cgttacgttg tagttaaaca
    22561 ggtccacacg ctgatatttt ggtgtgacgc caagagacca tttttgtccg gccgtttcca
    22621 gttctttggc aaatgaaatt ccgacgtcgg taattacagc tgcacgcccg aatgcgcgtg
    22681 aggtcagggc attcttatcg acgtcggtaa ttgttccatt ggcaactttg tccagataat
    22741 ccaggtctgc atcgtttact tttccgttta cgctgacagt tccgtaagac ttgaccataa
    22801 gggcaaacgg cagagtgtca tttgccatgg ctgctacagc tgatacacct acctgagcat
    22861 ctgcattgat attgcggaat tcctgcagac gatgtttaag atttgccgct gcctgttgta
    22921 cgccatgctg gttatctaca gcactgtcaa acaaatccca gtcatcttta acatcatcag
    22981 ctttatttga tacatcatca ggatcagcaa cttgtgcccc aacagacggc agaagaaggc
    23041 tgaaatcatc attgctgttg tgctttgtca gtaaagccgg gtttgccaga ggtgccacac
    23101 cataatggga agatgctacc ccggtgccac ccattgcatc gttacgcgct tcaaaatatg
    23161 acgtcgctgc tgcagcgtta aaggaaaata atacagcaga tgcaatgatg gtgcgggtaa
    23221 tatgattttt cttcatcatt taatttcctg tttatcagaa tttagacaaa acaacgctat
    23281 gacacggatg tgccgtaatt ttttactttg gtactatctg gtatttacca gggtaataaa
    23341 gacgtattaa ataagctaac aggtagtatt tgccctgtaa taaggttctc ggcaaggcca
    23401 ggtctcaacg tgacgcgtcg caaagacatt gttgtattct gcctggttgt atattcagtt
    23461 aaccattttc ttttttcatc gttcagaagt gttggtgata atgatagtgc cggatatatt
    23521 acagagccat ttatttcttt ttcctgtctc aatattaccc cgcaatattc tcttttcgtg
    23581 tttcctgcga aggggaaact atgccagaaa ataacttcag tgttgctgct atcattaata
    23641 agcattctga aattatcagc agtatctggc cattcaccgg acaacgctat cggatagttt
    23701 tcatggccat tcagccagtc cggattgaga ttaaaacatt cagcaagatg ttcgagagcg
    23761 tcagctgaaa gatagtctgc aagtctggcc gggtcctgta taaccgtgga gcggatattc
    23821 catggcgaca gcaatgttgc cagtaatggc gcatttatat catgcgacag catgatgtat
    23881 tcaattctgc ttattatgtt tcctgtggtg ttatccgcag gcataaacat attatgaaga
    23941 gcatcctcaa gcaggattcg ggttaaatca gcggtggata tggctaattg ttcgctcatt
    24001 atccccagaa cttctcttac cccttgtggg taacggacct gcagtggtgt cgggtttatc
    24061 tgacgaccgt tgatgagttt ttcgccgatt gcttttttgt ccagcgagag catcattcgg
    24121 gccgcaatac cggtcgtatt cagtctcatt gttcctccga ctgaaaaagt cttttcagct
    24181 catgtggtta ttgtatcttc ggtgtttttt ctggtgaaac gaataatttc gatcatgcag
    24241 atcgaatgtg tttggcgagt tgttgtaact cgcctttctg tatgtgtttt gaatgatgtt
    24301 tacagcggtg tgctgcccgg gttcagaggg caaatcataa tgtagtcagg gtcgttaaca
    24361 ggtaacgctc cgggagatgg gacacgttcg cctttctgtg ctgcaatggc cagtgtacgc
    24421 aacaaaacct cactggcgcg gtgaactgct tcctcttttg ttgtggcagt gacgtgaagg
    24481 ttatcaaaat caccgatctc aatacgatgc gctttctgcc cattttcttc atcgatagtc
    24541 gtgatatggg caggatacgg cacaaaccat gctgccactt tttgtgtctg ccgccggcgg
    24601 gcatcaagct tactgaccag ttgccatgca cgaagatgag tataccgttt cagcatattc
    24661 atggaacgat gtcctgatat tgcagcaatc tccattacat tcaggctacc cagttcgaag
    24721 aagcggctta ttgcttcatg ccgtagatcg tgaaaatgca ggtcctcgat gcgaagtcgt
    24781 tgtgtggcta ttctccaggc atttttaaag ccggatgcgg tgtaatcaaa aacattgccg
    24841 tggagattaa cgggcatcat ttgaagaaag ttacgggcac gtctggacag aggaacatcc
    24901 cgtgagtgac cgtttttggt ttcaggtaaa tgagccacac cgtggcgcaa atcaatgtgc
    24961 tcccaacgta aggccagtat ttcgccctgc cgcatggctg tttcaagggc aagatggaaa
    25021 atgacataca acatcagatt tttttcgcgg aaatagcgag aaaggcgacg ttcttctgaa
    25081 gacgttagcc ggcgatctcg tccggaggat actttcggct tgcgaaccag ttcaaccggg
    25141 ttagtacgac aggttcccca ttcaacacga gcaatattga acagagatga cagaagggcg
    25201 agttcaagac gtacagtatt acctgtaatg gctttacccg ttcgggggtt tatttctgct
    25261 aaacgaacgt ctctgtatgt agcaatatca acggttgtta tttcgtccat attccgaaga
    25321 gcaatgggat atcgcttgat aacattgctc cggtaaaact cctgttgatg ccctttcttg
    25381 tgaacagaaa ctgttttcag gtacttatcc agtgcgcgtg acagggacat tttacggatg
    25441 cgtggagacg gcatgctaac ctcaaaagag gcaaaaaata atgccaacaa tcattctctg
    25501 caa
//
LOCUS       DAZOXR010000047        25494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZOXR010000047 DAZOXR010000000
VERSION     DAZOXR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.047"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..485
                     /gene="gspA"
                     /locus_tag="ACHUNN_001635"
     CDS             <1..485
                     /gene="gspA"
                     /locus_tag="ACHUNN_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0576038.1"
                     /translation="SLQTLVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTW
                     TLTHKWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAE
                     WRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK
                     "
     gene            487..906
                     /gene="gspB"
                     /locus_tag="ACHUNN_001636"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="ACHUNN_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFR0576039.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUNN_001637"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUNN_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0576040.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUNN_001638"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUNN_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0576041.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUNN_001639"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUNN_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0576042.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUNN_001640"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUNN_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0576043.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUNN_001641"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUNN_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0576044.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUNN_001642"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUNN_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0576045.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUNN_001643"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUNN_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0576046.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUNN_001644"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUNN_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0576047.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUNN_001645"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUNN_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0576048.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUNN_001646"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUNN_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0576049.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUNN_001647"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUNN_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0576050.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUNN_001648"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUNN_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0576051.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUNN_001649"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUNN_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0576052.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUNN_001650"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUNN_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0576053.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUNN_001651"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUNN_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0576054.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUNN_001652"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUNN_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0576055.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUNN_001653"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUNN_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0576056.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUNN_001654"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUNN_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0576057.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUNN_001655"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUNN_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0576058.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUNN_001656"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUNN_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0576059.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUNN_001657"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUNN_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0576060.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="ACHUNN_001658"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="ACHUNN_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0576061.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUNN_001659"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUNN_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0576062.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUNN_001660"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUNN_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0576063.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUNN_001661"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUNN_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0576064.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUNN_001662"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUNN_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0576065.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUNN_001663"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUNN_001663"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0576066.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUNN_001664"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUNN_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0576067.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="ACHUNN_001665"
     CDS             14905..15273
                     /locus_tag="ACHUNN_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR0576068.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUNN_001666"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUNN_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR0576069.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUNN_001667"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUNN_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR0576070.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUNN_001668"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUNN_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR0576071.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUNN_001669"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUNN_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0576072.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUNN_001670"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUNN_001670"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0576073.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUNN_001671"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUNN_001671"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0576074.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUNN_001672"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUNN_001672"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0576075.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUNN_001673"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUNN_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0576076.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /gene="smg"
                     /locus_tag="ACHUNN_001674"
     CDS             21950..22423
                     /gene="smg"
                     /locus_tag="ACHUNN_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0576077.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="ACHUNN_001675"
     CDS             22452..22994
                     /locus_tag="ACHUNN_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0576078.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUNN_001676"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUNN_001676"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0576079.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUNN_001677"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUNN_001677"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0576080.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="ACHUNN_001678"
     CDS             24391..24648
                     /locus_tag="ACHUNN_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0576081.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="ACHUNN_001679"
     CDS             complement(24624..25178)
                     /locus_tag="ACHUNN_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0576082.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6023 c         6679 g         6300 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagtcagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaag
//
LOCUS       DAZOXR010000048        25466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZOXR010000048 DAZOXR010000000
VERSION     DAZOXR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.048"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            116..748
                     /locus_tag="ACHUNN_001680"
     CDS             116..748
                     /locus_tag="ACHUNN_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0576083.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            748..2004
                     /locus_tag="ACHUNN_001681"
     CDS             748..2004
                     /locus_tag="ACHUNN_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFR0576084.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            2031..3605
                     /locus_tag="ACHUNN_001682"
     CDS             2031..3605
                     /locus_tag="ACHUNN_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0576085.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            3627..4514
                     /locus_tag="ACHUNN_001683"
     CDS             3627..4514
                     /locus_tag="ACHUNN_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFR0576086.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENTRREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSAFLRDKVLMTPAKARVIYGSHFEVGDDGSVVGYDKP
                     AGHKDRAVLVDGEGNTLSFESAIERIVRADPEADAVMRSEAKQGAGSNSKPTHKVVQQ
                     KSKSTMDKLTSGLGKIGLK"
     gene            4540..5460
                     /locus_tag="ACHUNN_001684"
     CDS             4540..5460
                     /locus_tag="ACHUNN_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0576087.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREATLSEATFIDVNDTIAEGAATFSEHVAKLRILAGDVDVDKFLATTMSDTNNQL
                     AVQVRQKVKGLARAFRRNLILGNSETNTKAFDGIPRLMHKDQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGIPFIVND
                     FIPKNGVKVTEDSEQKTEGQEGTANIYCLHLSEENGLTGLYGGDNAGIVVENIGTVQN
                     KDAVRTRVKWYCSLANKHDKAIAALNNVKI"
     gene            5558..5992
                     /locus_tag="ACHUNN_001685"
     CDS             5558..5992
                     /locus_tag="ACHUNN_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576088.1"
                     /translation="MKPAKIRLLEPQFSDYSGMLCGVKFENGVSVSELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYGERNDLNVDQIVEPSAPDIVPMKRGTADEPAKPIQTFTRE
                     ELESIADSEGIAGLRLIGNKIGVKAKGIVEMIDGIMKAQGGE"
     gene            5992..6825
                     /locus_tag="ACHUNN_001686"
     CDS             5992..6825
                     /locus_tag="ACHUNN_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576089.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNTFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDIVAAREDGILSESIGETTNMYRTGKSANVHVARSTWRLLIRYIDNRMIVRR
                     S"
     gene            6897..7241
                     /locus_tag="ACHUNN_001687"
     CDS             6897..7241
                     /locus_tag="ACHUNN_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576090.1"
                     /translation="MNISWQTEIAIYRFGTKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLESAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            7232..7705
                     /locus_tag="ACHUNN_001688"
     CDS             7232..7705
                     /locus_tag="ACHUNN_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576091.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIEGVKPRVGPHFLERAVEISEKQFSEAILEAAKKAGFTRG"
     gene            7707..8090
                     /locus_tag="ACHUNN_001689"
     CDS             7707..8090
                     /locus_tag="ACHUNN_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFR0576092.1"
                     /translation="MFIEAFANLMQKAKIGTVGTDIFCHYLPAYVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTIEKARKIIDMFPVEETESGEVYFRLVRPMAMP
                     VVYPKNDGSLIEAGIPVEFAGYLLN"
     gene            8164..8901
                     /locus_tag="ACHUNN_001690"
     CDS             8164..8901
                     /locus_tag="ACHUNN_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFR0576093.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QQGQTVISELVQGRNITITAPLAESVLKNMVDLMPGSTLSEDDNSVTITSAQGVNLIN
                     VAKELVLTPQDTTDYVLTLPKAATAGNFTMSYQSDDVRVFSVQFNAYPDDEGVLGKMS
                     GPKPVKNVTITPESPSVKVGATVQLTANFTPDDVVDKTGVWSSDNKKATVDHTGLVRG
                     VEQGSANISFTSNSGSKKVTKLVTVNQ"
     gene            8969..9286
                     /locus_tag="ACHUNN_001691"
     CDS             8969..9286
                     /locus_tag="ACHUNN_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576094.1"
                     /translation="MTKLLDLDSILPPKKSVKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LTNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            9412..9636
                     /locus_tag="ACHUNN_001692"
     CDS             9412..9636
                     /locus_tag="ACHUNN_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576095.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARAAQADGEGVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDRADPDAKEQLMQIFGRG"
     gene            9644..14200
                     /locus_tag="ACHUNN_001693"
     CDS             9644..14200
                     /locus_tag="ACHUNN_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0576096.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAVGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLINKPVRVRTSGSGNGGGSGGR
                     GGYSWHIGKNNEGSMFSGLRGNIFLLGEIGDAARTVTSIMFGWQKPIIEAAAEMERMR
                     VMLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLK
                     ALVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGI
                     TMGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFAD
                     RVGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQEL
                     VINLGKVVAAMVAFKLMRAGIMGVVGAGSKMVSTFATMATAIQTPFNLGATAVTRFNR
                     AARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMT
                     MLRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNS
                     AGVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAEN
                     QIFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSF
                     SSRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFER
                     DNAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFE
                     GKPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRS
                     ANASQRAAKKEQTAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLT
                     DLANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSIIS
                     AGYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIK
                     ETGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDIT
                     NMVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGE
                     ATKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSM
                     QNLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSLTSSSAKT
                     ATSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAY
                     VPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMK
                     AIALETIAQEKRPGGSLNPNTQRN"
     gene            14243..14578
                     /locus_tag="ACHUNN_001694"
     CDS             14243..14578
                     /locus_tag="ACHUNN_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0576097.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            14661..15359
                     /locus_tag="ACHUNN_001695"
     CDS             14661..15359
                     /locus_tag="ACHUNN_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0576098.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            15352..16149
                     /locus_tag="ACHUNN_001696"
     CDS             15352..16149
                     /locus_tag="ACHUNN_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0576099.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLCRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            16137..16730
                     /locus_tag="ACHUNN_001697"
     CDS             16137..16730
                     /locus_tag="ACHUNN_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0576100.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVMMPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFD
                     GPQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            16747..21453
                     /locus_tag="ACHUNN_001698"
     CDS             16747..21453
                     /locus_tag="ACHUNN_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0576101.1"
                     /translation="MEQFKKKKLPLLIAGSGGKKSSKSSSRTPVEADDTVNSRAMASI
                     LDLLGEGVIGGLVNGARSIFVDDLPIVNEDGSSNFSGISWDFRDGSQDQTPMSGFDFV
                     ETPKSINIQLKKSHYVTVSIDNDEADRVRVIMKFPSLRRIDQKTGDTNGTTVEYKFQI
                     SNGDSTFVDVVAEGEKNVGIKLTAKKTGAYYRSYELKLPKPGRAYSVRVVRITDDNSG
                     QYLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGGSMPSRSYLVRGLKIRVPSN
                     YNEASNTYDGVWDGSFKLLSSSNPAWILFDLLTNSRYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETSGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THSGLGDSETWYYWVRSANKRGMLSRPNSNLGTEATTRDVLSFLANKITSSELGQALN
                     EEIDSKASQQAVDEINNHIDQSVESLEGEISALNKELTSSIDEFKTSFTERSETLENA
                     QSELRTEVSSTREKVDDALRQVENSNAALIELRNTVSKQGDSVAGVVEAANAALENAS
                     SLIAEERKVRAQADLANAKKIETMRSSVDESVAAVEEMRRTVAEVGRVSSEITTNLEA
                     ISKTNIDLALRQDEDQHRQLVNNAKIATTQKILVDDVSAMASKVEEIRAEIGDNIRAS
                     ILEEATARTDGDNALANRVTQLQSKFEGDISAAIRTEQSARASANETLSKQITQLESK
                     VNTDISTAIREEQQTRATQDEALSRQITQLQTKVNNDISAAIKSEQQARVSGDSALST
                     QINQLQAKVDDDISAAINEEREARASGDSALSREVSSLKTKTGKDIAAAVAVETKART
                     DANDALSRQISSLTTRANNLQSSIERESTSRTSQDSAIAKDLASFKTKTAKDISAAVA
                     VETQARAAADTALTSQINSLKTQTGKDIKAAVAEETKARTDADAALSTRVTNLQSQTT
                     NYINAAITSESKARTSADNALGKRIDTLKASVDGNVAVIQQQASAIADANRKVSTSYT
                     LKMETSTSGGQKYVAGIALGIDTTGLSQFLVRADRFGLVNNVNGKITTPFVIENSVAY
                     MNGAYIKDGTITNAKVGDLQSTNFVRGRAGWRFGKNGTLEINGNSGGNGRLVINGQRI
                     DVYDDNNVLRVRIGRL"
     gene            21548..24919
                     /locus_tag="ACHUNN_001699"
     CDS             21548..24919
                     /locus_tag="ACHUNN_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR0576102.1"
                     /translation="MWYREGTITFTQGSNTLVGAGTAWNVTANGVLPGMIVVAPDNKL
                     YEIKSVTNDTTLTLVENYTGETQSDIPCRIITTYEGDLTQFSARFTALLSRMSGDAKT
                     VRNWLTAVDEVTLEREDGTNVNVKPLSKIIAEHNKHVEWYENNTNAINSAGDKAKQAA
                     ASASAAAKSANLASSKATQASNSALDATASKNAANKSASSAKASETSALEAKNAAAIS
                     ASTASTKATEATSSANAALVSKEAAKLSEMNAASGAGSASTSANQARESARSAKTSAN
                     QASTYASAARASKDEASRKSAEASTSASNASSKSAESAAKAEEAKQSQTSALASAERA
                     EIAATRAERALSAISLHDASLTMKGIVKLSSETSSSSEEFAATPKAVKAAFDLAKSKY
                     TANDATTSQKGLVQLSSDTNNASESLAATTKAVKSVKDLADRKAAINSPAFTGTPTAP
                     TATQGTNNTQIATTAYVREAISGLVGSSPEALDTLNELARALGDDPDFATTMTNKLAT
                     KQPLDATLTALAGLTTAANKLPYFTGNDTAGLATITSVGRDILSKASVQAIIQYLGLK
                     ENGTSGDKIPLLSTANTWSAKQTFSNGLAGSLNGNSDTATKLKAARKIGGVPFDGSSD
                     INLPGVNSTGTQSTSGNAASATKLATPRSIGGMLFDGTANIDLPGVNKVGNQSTTGNA
                     GSATRLQTARTIGGVSFNGTANIDLPGVNKAGNQNTSGNAATATKLATARKINGVSFD
                     GSKNISITPKQLGAAKYVANLATGNTQEWTTAQFVAWLNSQGAFDATQWSTRCSWSYG
                     SNCYIGNASTGCGVIPLAGSVIEVFSTGTSNYTIRITTPSTSGGASGAVLNSEFIYTY
                     NGDNYSPTWRRNYSSQNKPTASEIGALASNGNAVSATKLKTARNIGGVSFDGTANIDL
                     PGVNKAGNQNTSGNAATATKLKTSRTIGGVSFDGSANINLPGVNTSGNQNTSGNAASA
                     TKLQTARTINGVSFDGTKNISISAAQIGALPIGGGTITGNLTVNGNGSFNDVQIRSDK
                     RNKRNLVKLDNALDRLEALTGYLYEIQLSDDSWKTSVGLIAQDAQKALSELVTEDTDV
                     ISGEKRLCLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            25005..>25466
                     /locus_tag="ACHUNN_001700"
     CDS             25005..>25466
                     /locus_tag="ACHUNN_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576103.1"
                     /translation="MGVASGWVGSSAKSETGEQWMSAAGTKLGLGKPFMMSQMVGRSM
                     GCKIATAYYKGNPPDKVENWGAVGSDWPLAEKNQGTIAGASSCGLGRLVGIVLTQAHY
                     VPGLPPTGGLYIAGGRASNITVTVGGVSQVFTYNSVINGFHYYWQGTPSSAF"
BASE COUNT         7450 a         5246 c         6478 g         6292 t
ORIGIN      
        1 tagtaagaca tgcccagacc taattaaaca ccccagaaaa caagttggtt agcatatgca
       61 gtaaacaagt tgttttagag cgcatggacg cgctctgtgt gttttagagg gatctatgac
      121 ggtcgaaaaa gacgaggtaa aaactcgcct gacaccagcg gagtgggccg aagctgaagc
      181 caaatggacg tcaggcgaat atacactctc aaagctggag gaagagtacg gcattcgtcg
      241 tgaaacactc tccagacatt tcaaaaagcg aggattagag aaaggcgcgg actctgttgg
      301 gaagatggtt cgtgagtctc ttaaatctga cgcagagctt cgcgctaaag cccgtgcgga
      361 aaagatagaa gaacgtcgta cacgttatga cggctgggcg tatgcgttgg ggcagatggt
      421 gatggtcgaa gtcactacgg ccaaacgtga gggtaagcct ttaggggcga ttgaggattc
      481 tctcaagagc ttacagagag ccagtaatac tcttgcaaaa tgctttgaag tttcgtccaa
      541 agcattgggc atggatcatg cggaaaatga cgaggaagaa attccgaacc tggtatttgg
      601 tgagcttacg ccttcccagg tggcgaaatt acgtcaggaa gacgacgagc ctgaaatcat
      661 cgatgacgaa ttgcttgaga cgttggaaga agaagctcta agcgaatttg atgcgacaga
      721 tgatggaagt gaaggggagg acgaataatg gcaatcccgt cctcgctcag tcttgtgcaa
      781 ctgcattctg gacagatgaa agtcttccag tctccgcatc gatttaaagt tgtttgtgct
      841 ggtcgacgct ggggaaaatc ccggttgtcg atctccacta ttattcgtgc ggcggcaaag
      901 gaaaaaaagc aaagagtctg gtatgtcgct cctacttacc agatggctcg ccagattttg
      961 tgggacgatc tacaggaagt tctgcctcgt aagtgggtta ggaaaaagaa cgacaccacg
     1021 atgacaatcg tgttgaagaa cggttcggag atcgccctca aaggtgctga taagcctgac
     1081 actctgcgcg gcgtagcgtt gcattttgta gtgcttgatg aatttcagga tatgaaggct
     1141 gacacctggt acaaggtgtt acgacctact ctttcatcga cacgcggcgg tgcactgatc
     1201 attggtacgc caaaaggctt ctcggaattt cacaaactgt ggactatagg ccagaacgta
     1261 gagctgcaaa gaaagggaca gtggaagagc tggcagtttg taactgccga ttctccgttt
     1321 gtacctacgg cggaaattga agctgctaag aacgatatgg acccgaaatc gttcgctcag
     1381 gagtacctgg cgagctttga gaacatgtcc gggcgcgttt actacccgtt cgatcgtaac
     1441 gtgcatgtaa aaccgcttca gttcaaccct cggttgccta tatgggtagg gcaggacttc
     1501 aacattgacc cgatgtcttc agtaattttg caacctcagc caaatggtga gctatgggca
     1561 attgatgaat tggtgctctt ttcctctaac acggcagaag tttgtgatga gcttgagaga
     1621 cgcttctggc gctggaaatc acaggtaacg gtatttccag atccggcagg tgcttatcgc
     1681 caacatgctc gtggggagtc tgacgtagac atattcaaag agaagggatt cttacgtgtc
     1741 gattattcga aaaagcaccc gccaattgcg gatcgtgtta atgctgttaa ccgaatgctg
     1801 atgtccgcat ctggagatat ccggctgtat atcgatccga agtgcaagca tttgattgat
     1861 tcactggaaa aagtcatcta caagcctgga acacgagata tggataagac aggtggcatt
     1921 gagcatagtg cagacgcatt gggctatcca gtacatcgta ggtatccagt aaaaaatcgt
     1981 gttattcttg gtggttctcg ataggtaagt aattatctaa ggttattcaa atggaattga
     2041 acgacaaaca aattaaggat ctggtggcgc gacgccaccc ggaatacgaa aagaaaaaag
     2101 aacattggga cttcctcgcc agcacttacg ctggcgggcg tggttggttt acagacaata
     2161 tctttcgtta ctttaaagag ggagatcagg agtttaagga gcgagttgaa cgtgcttatc
     2221 gcttcaacca cactcgtgag gtggtaaacc tcatcaacaa atatctcttt aaagaagaca
     2281 ttcatagaaa tatcgaagag gcaccagagc agatccgcaa tttctggaaa cgtgcgactc
     2341 gccagaatgc ctctattgac tcatttatgg ccgctattga tttgcagtcg tctatttacg
     2401 gtcgcatatg ggttgttgtc gatagcacga tgagtggtga tgttgagtca gtagctgacg
     2461 agaaaaagaa agatgctcgc gcctacgcct actggatttc acctcagcaa atgctggatg
     2521 tggcatggga cgacgacggg aatatgttgt gggcgttaat tgtggaagtc gctcgtgatg
     2581 acgcagatcc ttttacttct acaggtcagg aataccaacg ttatcgtctg tggacacaaa
     2641 acgagtggta tctgttccgt gaggaagtga agaagggcgc tggtggagca ggtcgccgtc
     2701 aggcaaaagt tattttagag gatagcggtg agcataatct cggcgtagtt cctgtgtttc
     2761 ctgttgattg tattggagaa agtgaatcac cgtatttcag cccatcgttg atcgatgata
     2821 tcgcttatct tgatcgtgcg gttgcaaact atctgtcaaa ccttgatgcc attattcagg
     2881 atcagacatt tagccagttg gctataccgg tacagtcgct tttacctggt gatgaaaacc
     2941 acactaaagt gcttgaaatg ggcacaaagc gagtcttcac ctacgattct gaaggtggaa
     3001 accagccgtt ttatctgtca ccagacccga aacaagctca gatgatcatc actacgatta
     3061 agacggtgat taacgaaatc taccattcag ttggtgtagc tggtgagcga acgaagcagg
     3121 acaacgcaca gggaatcgat aattcatctg gtgccgcaaa aatgtatgac ttccagcgtg
     3181 taaatagctt gcttgtcaca aaagcagagc gtctggaaag ggctgaacgc caaatcatgc
     3241 tactggttgc gaaatggatg ggggtagatc tggacgaaga ccactcttta attgcgtatc
     3301 cagaaagttt cgatattcgt ggccttactg atgaattttc tgttgccgag aaactgtcat
     3361 tacttcaggc accggactct gtacgtcgtc accagatgga aatgcttatt gagaagattt
     3421 tcccgaacat tactgaggcg atgaaaaagg aatttgataa agatctcttg aattttcctc
     3481 caaaaaatga tctaaatacc cttgaaaata agtcagtact tacttatgat cgtggtgcag
     3541 cccaagaaag cgggcaagat caaccccgag ggaatgggga ctcatctact caagagaacg
     3601 agtgataagt aacaaaagga atttttatga atctgtggca aatgcttttg gcccgtcgtg
     3661 gtctgatgga tgtcgctgaa gcgcatgagc gtggaggcgc tggcggtgta gctgctgata
     3721 atgagcagag tacacaagat cctgacaaac agggtgaaca aaaagagcag ccgaagggcg
     3781 atgacgaata cgctggcatg actcaggaag agttactggc cgaacttcgt aaaaccaaga
     3841 aagctggtgc tgaactgctg aaggagaaca tgaagcgcaa agagaaagag cgcacattgg
     3901 ccgatcagct tgctcagtac ggtgatatcg acccggcgcg tgctcgccag cttttagagg
     3961 ctgaacaggc cgcagaaaac acacgtcgtg aggcggagca agctgaactg gagcgtcgtg
     4021 gtgagttcga tgctgttaaa aaacagatga tcgaagcaca ccaggcagag ctggcacagc
     4081 gtgacgaacg ttatgcagca ctggaaagtg aaaacgcatc actgaaatct caattagttg
     4141 agatgaccgt tggcgcgtca tttagtaatt ctgccttcct tcgtgacaaa gttctaatga
     4201 ctcccgcgaa agctcgcgtg atctacggtt ctcattttga agtcggtgat gacggtagcg
     4261 tagtgggtta tgacaaaccg gcaggccaca aagatcgagc tgttctggtt gacggtgaag
     4321 gaaacacgtt gtcatttgaa tctgcgattg aacgcattgt gcgggcagat ccagaggctg
     4381 acgcagtcat gcgcagcgaa gccaaacagg gtgctggttc taatagcaaa ccaacccata
     4441 aggtagtcca acagaagtct aagtcgacga tggacaagtt gacctctggt cttgggaaaa
     4501 ttggacttaa gtaacatcta aatcaaaggg aattgataga tgccattact gcgtgaagaa
     4561 gctgaaaagc tatccaataa tgaacttgaa cagggtgtga tcgaaactat catcgatcgc
     4621 gatgacctgt ttgcgattct gccttttatg aagattaact cgaaggcata tctttataat
     4681 cgcgaagcaa cgcttagtga agcgaccttt attgatgtaa acgatactat tgcggaaggc
     4741 gcagcaacat tcagcgaaca tgttgcaaaa ctgcgtattc tggctggtga cgttgacgtt
     4801 gataaattct tagcaacaac tatgtccgat actaacaatc agttggctgt tcaggttcgc
     4861 cagaaagtta aaggtctggc tcgtgcattc cgtcgaaatc tgatcttggg taattctgaa
     4921 actaatacca aagcgtttga tggtattccg cgtctgatgc acaaagatca gaagatcgac
     4981 attgctggtg catctatgac tttctctatg ttcgacgagc tggtcgatgc ggtgaaagat
     5041 ttgggcgctg actgcatcat gatgcgttca gagcacctgc gtgcttaccg tgcgctgctg
     5101 cgtacagtaa acgtaggccc gtctgaaatc atgatggaga acttcggtcg tcctatgctg
     5161 tgccataatg gtattccatt tattgttaac gacttcattc ctaaaaatgg ggttaaagtt
     5221 actgaagatt cagagcagaa aacggaaggc caggaaggca ctgctaacat ttattgtctg
     5281 catttgtcag aagaaaatgg tcttaccggt ctgtatggcg gtgacaatgc tggtatcgtt
     5341 gtggagaaca ttggcacggt acagaataaa gatgcagtac gtactcgtgt gaaatggtac
     5401 tgctctctgg cgaataagca cgataaggct atcgctgcac tgaacaatgt aaaaatttaa
     5461 ctaaaataat aggtgtgtaa ttacctatgc ttaagggtgg gctatacgcc cacccttttt
     5521 gtaggtttta aatatgcctg tatagcgaga aagcgtaatg aaaccagcaa aaattcgttt
     5581 attggaaccg caattttctg attattcggg aatgttgtgt ggagttaagt ttgagaatgg
     5641 ggtatccgtt tccgagttac cttttatcga ccagcagagg atttgtgcat caatgcgcgc
     5701 atcaactgtt gagggtaaaa acgtttctcc atctgctgca tatggcgaac gtaatgatct
     5761 gaacgtagat cagattgttg agccatcagc acctgacatt gttccaatga agcgcggaac
     5821 ggcagatgaa ccagctaaac caattcaaac tttcacacgg gaagaactgg agtcaattgc
     5881 tgatagtgag ggtattgccg gtttacgttt aattgggaat aagattggtg ttaaggcgaa
     5941 aggaattgtc gaaatgattg acggcatcat gaaagcacaa ggcggtgagt aatggcgcag
     6001 atcgactcgt atcgtagcgg tgaagctgtt tctctttcat ttgccttcaa cgtgctggat
     6061 atcgaatctg ccacctacac agtaaaggac agcaccgggg ctattctcgt tgatggcgag
     6121 ccgctagaaa ttaccagcgg tcaaatgtcg attccggttg tcgtgtcggc tgaatataac
     6181 cagctctctg agaaagaacg agatctgcgg tacgtcattg tgaaggctgt tgcatcgggc
     6241 cttacgcatg aagagcgtca aatgtatgtt ctgctgaata cttttgaact gtcgatacca
     6301 gaacagtcgt ttgcaactgt cgctgatgct caaatgcagg cgatcgatat gctgaatggg
     6361 gacactttgc tgtcggatgg tgaaggctta atgcgcaagc gtctcattga ggctacaaga
     6421 cgtattaaaa ctttaccgtt ctcaatccgc aaaattctac gtatcgactt tgaccgatac
     6481 gatcgccctc aaaatatgct gaatgtgtat gacattccgt ggggagcaga tggagcgtac
     6541 cggcatgatt tagtcgattg ggaaaagatg actcaggaga agtttgaaga gttccctgac
     6601 tacttcaaag aagcgttaat gctagccgtt gttaatgaag cgtgtgagat agcaaatggc
     6661 aatgatatcg tcgcagcacg agaggatggc attttgtcag aatcgatcgg tgaaacgacc
     6721 aacatgtatc gtacaggtaa atcagcaaac gtgcatgttg ctcgcagcac ctggcgttta
     6781 ctgatccgtt atatagacaa tcgtatgatt gttcgacgtt cgtaatgtct ttctcattat
     6841 ttggttttgg aagcaatagt tttggctggc aatgagatcg acaacaggga gagagcgtga
     6901 atatttcatg gcaaacagaa attgcgatct accgctttgg cacaaagaac gtctacggtg
     6961 aagcgcaatt gcagttcgtc aggaagacga atgtcggtgt agttaaattc gaacagagta
     7021 atgagaaatc gtcagttcgt gctgatagct ctggtagtcg tggcaaggcg agtctggagc
     7081 tgtttgatgc tgtgttagtt gtccctttgg aatcggctgt acaacttgat gatgtactga
     7141 tccttgaagg tcaaaaatta aaggtgtcca gtgttcatcg ccgctggggg ctacgaggaa
     7201 gacctgggca tcttgaagta ggggcgaata tatgggtctg aaatacgatg cgcatcagtt
     7261 caaacgtgcg gggaatagac tcaacaacag ccagaaagca tttaagcgat atcttattcg
     7321 ggatatggaa aagctggcgc gtttggttga gcgcctcagc cgtgcaatgg ccccgctgga
     7381 aaccggatct ctggagacag ccatctttgc tagggtgatt aaagaaggtt attcagggct
     7441 gcgcattgag ctttctgtgt ctggagcaaa gccacgtcag gggcatcctg gcgtagaagt
     7501 aggtgactat gctaagtaca tggagctggg caaataccgt ctcggttatc tttctcgaat
     7561 gaagaacgtg acaaacccac caattgaagg tgtaaaacct cgtgttggtc cacactttct
     7621 ggaaagagcg gtggaaatta gcgagaagca gttctccgaa gcgattcttg aggctgccaa
     7681 aaaagccggg tttacgagag gttaatgtgt ttattgaagc atttgcaaat ttgatgcaga
     7741 aggcaaagat tggcacggta gggacagaca ttttctgtca ttacttacct gcctatgtca
     7801 aatccggtgt cctgcttatt aacccaaata ccggtatcag tatcgatcat gagttacagg
     7861 gcttttatca tgagtcgttc acaatcattg tgcgtggctc gtcaattaca acgactattg
     7921 agaaagcgcg taagatcatt gatatgtttc ccgtagagga aacggaatct ggagaggtct
     7981 attttcgact tgtacggcct atggctatgc ctgtcgttta tccgaaaaat gatggatcgt
     8041 taatagaagc cggtattcca gttgaatttg ctggctattt attgaattaa ataaataggt
     8101 aagtatatac ttaaatatag cgcaatgaat gcgtgaaatt aacggaaaaa ggagtttcca
     8161 tcaatgtcta atacccatgt aaaaaacatc aagcttggtg cctgcaaggt atcatttggt
     8221 ggtgtggatc tgggttacac caaaggtggt gttcaggttg aagtagccac tgaaacgctt
     8281 aaagttacag tcgatcaaca aggtcagaca gtaatctctg aattggttca ggggcgtaac
     8341 attaccatta ccgcacctct ggccgagtct gtgctcaaaa acatggtcga tcttatgcct
     8401 ggttctactt tgagcgaaga tgacaactcc gtcaccatca catctgcaca gggcgtaaat
     8461 ctgatcaacg tggcgaagga actcgtcctg acaccgcagg acaccaccga ctatgtgttg
     8521 acgctcccga aagcggcaac cgctggcaac ttcactatgt cctaccagtc tgatgatgta
     8581 cgtgtgttct ctgttcagtt caacgcgtac cctgatgatg agggggttct tggcaaaatg
     8641 agtggcccaa aaccggtgaa aaacgtgacg attacacctg aatctccgag tgtaaaggtc
     8701 ggggcgacag ttcaactgac tgctaatttc accccagatg atgtagttga taaaacaggc
     8761 gtatggtcat ctgataataa aaaagccact gttgatcaca ctggtcttgt gcgtggtgtg
     8821 gaacaaggtt ctgcaaacat atcattcacc agcaatagcg gtagcaagaa agtaaccaaa
     8881 ttagtgacgg ttaatcaata atcaagcaac ttattaatct ataaattgag gctcatgatg
     8941 ggcctctctt ttaaaaggat tttaacaaat gacaaaatta ctcgatctcg actccatcct
     9001 acctcctaaa aaaagtgtca aatttggcgg ccaggaatat cccatcgttg aaatgacggt
     9061 aggtctgttc gtttccatta agcagatgga aggtaaagat ctgaccaata tgtctcctgt
     9121 tgagcaagtg acagcttatg cagatctggt tcgcaaggtt atcccttcag tgcccgacga
     9181 agttcttgaa aaactgactg ttccgcaact tcagcagatc ttcaccttcg ctatggaagt
     9241 gattgatgaa gaaaacgaaa aagcggctgg cgaaggggca aagtaatttc ccgcgatgaa
     9301 tccgggacaa ggaccgtctc aatagatctc ggattctatt tcagtcgtat agttgctcac
     9361 tacgccgtgt cgccgttaga actactgaac atccctctca cgatgttctg gatgctcagt
     9421 cgcaacatcg accgtctacg tgcggaagag gatgtccgca acctgcaagt cgctcgcgct
     9481 gctcaggcag atggcgaggg cgtgaaggcg ttcatggagg gtttgcaact caggattgga
     9541 agaccagtcg taacagataa agtctacgat ccaagccagg atagggcaga ccctgacgcc
     9601 aaagagcaac tgatgcaaat atttggcaga ggatgacaag ggaatgtcac aaaacgtaga
     9661 gtttatcctg tcgctggaag acaaacagtt tacagcgtca atcgatcgtg cgggaaaact
     9721 gcttactcga tttggtgagc aggtaacaaa gcctgctcaa aaaatccaaa cctttgaacg
     9781 ctctttgggt tcggtcgccc gtatcattgg cgttctggaa agcaagctcg attctacggc
     9841 agataaacta caggatgtag ctgtcggttt tgagcttgct tcagatgcta cgcgtaaaat
     9901 gcgtggcaac atcaccagcc tcaattctgg tcttaaagct ctgattgagc gcgtcgatac
     9961 gacaacttct tcagtagata aactcaccgc gtcattacgt aaagtgcaat cagagctaaa
    10021 tgatttctct gactgggcga cctatgcaag caaaagcgca agccgttttg gtacggaggt
    10081 caaagaagcc tctgcgtccg tgagtggcat gaatacgcgc cttaacacca cgacgaagcg
    10141 actcagtaat tggggtgtga caacaagcca ggctgccgag ggactaaaaa aagttcgtga
    10201 gcagatggac gaagttatcg gacgtcagca actcattaac aagcctgtac gcgttcgcac
    10261 atcaggaagc ggaaatggtg ggggtagcgg tggacgtggt ggttactctt ggcatatcgg
    10321 taaaaataac gaaggcagta tgttctctgg ccttcgtggc aacattttcc tgcttggtga
    10381 gatcggagat gctgcaagaa ccgtcaccag tatcatgttt ggttggcaga agcctattat
    10441 tgaagctgcg gccgaaatgg aacgtatgcg ggtgatgctt cgagggttga ataaggagaa
    10501 gtccaacccg ggccaggctg ccgctgatga tatgaagtac atcgtagaca tggctcaaaa
    10561 tgcgccgttc gcgatgcagg cgttaacaga ctccttcgtg aagtttcgtt ctgctggctt
    10621 agatccaacc gatggttctc tgaaggcgct ggtggactcc gttgctcgtt ttggtggtga
    10681 tagtgagctg ttgaaacgtg cggctgtggc cgttcagcag atgtccggta agggcgttgt
    10741 gtcaatggaa gagctacgtc agcaattagg tgaagccgtt cctaacgcga tgcaggcaat
    10801 ggcagacgcc gcaggcatca ctatggggga actgactaaa gccgttgcca gcggtacggt
    10861 tgaggcaaaa caggcgttgt ctctgatgtt tgttggtctg cgtgcggaga atgagaacgc
    10921 agcgaaagac atgatgcaaa cctacacagg tgcgctggcg caacttcaga cgtcattcac
    10981 gttatttgct gatcgggttg gtcaggccgg atatctggat tctctatcga aggggatgaa
    11041 agaactggct tcaatcatga atagcgccga agggatttcg tttgccaatt ctttaggttc
    11101 ggggttaacg acggcaatcg atgggttgcg tcagcttgct caatggttag caaagaacca
    11161 agagctggta attaatctcg gtaaggtcgt ggccgcgatg gttgcgttca aactgatgcg
    11221 agcagggatc atgggagtag ttggcgcagg cagcaagatg gttagcacct ttgccacgat
    11281 ggcgaccgcc atacagactc catttaacct cggcgctaca gcagtaactc gattcaatcg
    11341 tgcggcacgt atggggctgg ctccgatccc ctctcttatt ttcgccatcc gtggggcgat
    11401 tacggggctt aaaggcgctt ttgctggatt aacggcgttc attgcagcaa atcctatagg
    11461 ggcagcattt acagttgcca ctgtagctgt tgctggccta atcacgtaca tgaccatgct
    11521 ccgcagcgaa acttcaaagg tcgttgacga gattaggaaa ataccagagg cgatgacggc
    11581 ggccaaacgt gcacaaatgg ccgaccgcgc agaaaagctc gaaaagcaga ttcttgctga
    11641 tcaacgagca ttaaaaactg gtgagggtgt taattacgta tctaactctg ccggtgttat
    11701 tactcacaaa gagtcgaagg ctgacatcga agcacgcctg aaaaaaaatc aggaagagta
    11761 tcaaaagata accggcacga tagctcttgg tgacggcgct gtagcaaagc gtttggctaa
    11821 agaggctgcc gaatctcaga ttgagaaaat tcgagcggaa aaccagattt tcgcggcaac
    11881 attcgtgaaa gcccggcagg aggctctgga taagatccag aaaatcaatg atgacggttc
    11941 actttcagat gacgaaaaga acaagctatt ggcaccgtta cgtgaaacgg taaacaaaag
    12001 ctatctggag cctgcgcaaa aactggttga ttcattttct tctcgtaaga atgctaccga
    12061 gaagcaaata gcgactctta atgatcagct tgaaaaagcc aaaaaggatg gaaataccga
    12121 gcaaatccag aaactgcaag gcagtatccg tggttatcag gagcatttgg aagccgttgc
    12181 tcaggaactg acacaggcag agttcgagag agataacgcg gccaaaactg gtaagggcgt
    12241 aatgtcaaac caggggactg ttcttgggtt aggtacaact gataaagctg ctcagaaggc
    12301 gctggcgcaa tatatgcgaa accagatgga ttctgcgact tatcaacgta ctttgcctga
    12361 cggcactccg atgatggact tcgaaggtaa gccgattatt gggcctaaac aactcaagac
    12421 gcagcttaat ttgcagaaag catccagtgc cagctctctg gagaaaatga gcgatgaaga
    12481 gcgtgccgca gccattgccg cactgactaa agctcgtgaa caggatgccg cagccgccga
    12541 gaaagcagcc caacgatcag ctaatgcctc gcagcgtgcg gccaagaaag aacagacagc
    12601 gcaacagaaa ctggcagccg gataccagaa ggctctggat aaagccgatc agcttatggg
    12661 gcaaatgggt gaaagctcta aggctacggt atcgtttgat cagtctcttc gcgatacaac
    12721 gaaatcgctg acagatttgg ctaatgcggt tcctaacgag ttcatcactc aagagatgat
    12781 cgacaaggcg aaaaaacgtc ttgaagacct caaaaatgcg acacctgaat atcgcgagat
    12841 gtttaatcgc cgcaatgttg agcagatgat ctccacctgg gcaccggagt cggattccat
    12901 tattagtgct ggctatacgc cgtctcgtga agagaaagtt gctgatttcg aagacaccta
    12961 caaccgcaat ctcaaagcgt tgatagaact tcgtgataag gcgtctgatc ctaaagttgt
    13021 ggcgctttat acaaagaaaa tcaatcaact gattgctgct ggcaataccg cgcttattaa
    13081 agagacgggg actgcgacgc agaagttggc actggaatac gaaaacctgg cagagcagat
    13141 cgaaagcacc tggactgatt tgtttagtgg cttaactgat gtcctgactg atttcgttat
    13201 taacgggaag atgagtttct ccagcctatc tcagtccatt ttgaaagata tcaccaatat
    13261 ggtcgtgaag tcgcaaatca cgctgcctct aatgaacatg ttggggatgg gaaccaccgc
    13321 agctggtagt tcacagagtg gtaatttgct gaccggggtt gcttccgccg ttgccaatca
    13381 aggggtacga atgggcaaca ctgttaacgg cgacaagtcg gtaggagaag ccacgaagga
    13441 gacgtccagt tcggtaactg gattagggca aacaacacag cagaccacca gcgcaattgg
    13501 cactgcaaca aatgcgattg gtagctgggt atcagggcta tttgatagca ctgaagccaa
    13561 agatgccgag acaaaagccg tgaaggactc catcttctcg atgcagaacc tcagctctgt
    13621 taccggcgcg ctgtctgccg cgtttgcaat gcttggagct aatgcttccg gctctggtaa
    13681 taagtggttg agttttggag caacggttgc atctggtctt gtttcagctt gggctggcgg
    13741 tggtttcgac agcttgacat caagctctgc taaaaccgca accagcagtg tggctgacgg
    13801 aactaaaggc attcctgcaa tcccgaagtt tgcaaatgga ggaatattcg gaaaagacgg
    13861 cgtgatcccg ctccgggcat accagaaagg cggtattgct aactcgcctc aattagcgtt
    13921 gtttggggaa ggttctatga atgaggcgta tgttccattg cctgatggcc gaacaatccc
    13981 tgtaacgctc agtaccgatg gtatgagtgg aggcggaaat gttctttctc ctgtatcaat
    14041 tgagatcaac gtccatagtg acggtagcac aactgaatcc ggcgatacag aaagcatatg
    14101 gaacaatgcc gctcaacgga tgaaagcaat cgcgcttgag actatcgctc aagagaaacg
    14161 ccctggcgga tcactcaacc caaacactca acgtaactaa ctatcgactg ccccggtcgg
    14221 ggcagtctca caaggatgtg agatggaaag acaaacgttt aattggtatc cagattacga
    14281 atctgaaaag agcgtaaaac cgaatgtaac ggtacttaat tttggtgatg actacgagca
    14341 gcgacaggct caaggtctta atcgtattaa agaagaatgg tcgttaacct ttaccagatc
    14401 atacaacgaa attaatgcaa tcgatgactt ccttactgag cgatcaggtg ttgaatcgtt
    14461 ctattgggtt aatccaagag gcaagcagat tgtagttgta tgtgacagtc atacggtcaa
    14521 gagatatcag gggtactgtg tcttaactgc tacattcaga caagtatttg aggcttaagt
    14581 atctggataa gtaagtacta atttactatc attgtggcgc tgacaggatg tcagcgccta
    14641 cttatttcaa ggatgaaaca atgggaatta aagctgatat tcagagctta tctccctctg
    14701 cactcattga gttgttcgta ctggatatgt cgaacacaac ttcagggggg aagctattct
    14761 tccacgccgg aacaaacgaa ctgatgcaac cggtcgtctg gcaaggagtg acatacgagc
    14821 cgtggccaat caaagcatca ggctttgaca aaactggcca gggaacgttg ccacgtccaa
    14881 aaattcaggt atcgaacttt gccggaaccg tctctgcgga agttcaggcg aacgacgatc
    14941 ttgttggctg ccgcattatt cgcaagatga cgctggctcg cttcctcgat gccgttaatt
    15001 ttaaagacgg caacccaaca gcagatccaa accaacattt cccggatgaa atgtggttta
    15061 tcgaacagaa aactctcgaa actcatcagg ttgtcgagtt tgaattgtcc agtgtgttcg
    15121 atttgatggg ggtacaactg ccgtatcgtc agatcattaa aaacacctgc ccgtggaaat
    15181 accgagggcc agaatgcggc tataccggtc catatttcga caaaaataac cagcagacgt
    15241 ctatgtctgg tgcggattac tgcacaaaac gttatgacgc ctgtaatgcg cgtcggaatt
    15301 attttgccga cggtgtgatc cattttggtg gatttattgg agctacgcgg tatgggtaat
    15361 aaagcaatcc ctgagcttgg ctctgacgtt atgcagcaaa tctatctctg cgccataaat
    15421 cgctacccta atgaagcgtg tggctttctg gttagaacta atggcgacaa atatcgcttt
    15481 atggaagcgc ggaatgtttc ggagaacccg cagaacactt ttgtaatgca cgttgacgac
    15541 attatggcgg ctgaggatgc gggtgatgtt atcgcaatct ggcattcaca tactgatgaa
    15601 tcagcagaag catctgatgc cgatcgtgca ggctgcgaag cgacggaagt tccgtggatg
    15661 attctggcta ttcgcaagaa tgttgaggga gatgcccctt tccattttag cgagatgaat
    15721 gtgatcacac ctgatggttt cgaaatgcca tacctgtgca gaccgtatgt atttggcgta
    15781 ttcgattgct ggatgttgtg tcgggactat ttgaagcgtg agtttaacgt cgaactaaac
    15841 ccgaacgcac acctgcatat tccatcgtgg tacaccggcg ataacgacat tctcgaccag
    15901 aactaccgaa acgaaggatt ggtacggctt gcgcccggaa cagaacctca acgtggggac
    15961 gtcttcttta tccaatacgg aaaaatgcct gaccactgtg cggtttatat cggcgatgga
    16021 atgattcttc atcaccagat cgaccgcctt agttgtcgtg cttattacgg cgggatgtat
    16081 cagaaacata cgacgcatca cttgcgtcac agagacttgc tcaagggaga tgagacgtgt
    16141 ctgagttagt tcatgtgcag cttggcggcc ctatggccaa acattttggc cgccactggc
    16201 atctaaaggt gcgcaataca aaacaggctc tggatttaat tgaggccaat aagcctggtt
    16261 ttaaagcatg gatgaagcgc aatatcaaaa cctatgacag ataccacatc cagatcacca
    16321 ataaacaggg ccacaagtgg tctgtggacg agagtgaata tcagatgatg gggcagtctg
    16381 acaacattgc caaaatccgc attacccctg ttccgcgagg aagcggcgga tctgcttttg
    16441 ggtggtttca gacggtagta ggggccgctt tgttggttgt atcggcggta atgatgcccg
    16501 ctcttgcccc tctcggtttg tcactgatga tgggcggtat agcgcaaatc atatctccgc
    16561 aagccactaa cgaaagtgtg aggcaggcgg ataactcgaa ctcttattac tttgatggac
    16621 ctcaaaacac aacaaaccag gggaaccctg tacagcttat ctatggcgag gaaattttag
    16681 ttggctcaca ggtcgtgagt tcttctatca ccatcgacca gcttatgtaa tcaaggattt
    16741 ttggacatgg aacagttcaa gaagaaaaag ttacctctat taattgcagg ttctggtggc
    16801 aaaaaaagta gcaaaagctc tagtcgcaca ccagttgaag cagacgatac cgtaaattct
    16861 cgtgctatgg cctctattct cgatcttctt ggagaaggcg taattggcgg cttggtaaat
    16921 ggcgcaagat ctatttttgt cgacgatttg cccatagtaa atgaagacgg ctcttcaaac
    16981 tttagcggaa tttcatggga ttttcgagac ggctcgcaag accagacacc aatgtctggc
    17041 tttgatttcg tcgaaacacc taaatcgatc aatatccaac taaagaaatc gcattatgtg
    17101 acggtttcaa tagataacga tgaggccgat cgtgttcgag tcatcatgaa gttcccttct
    17161 ctgcgaagaa tagaccaaaa aacgggtgat acaaacggca ctaccgttga gtataagttc
    17221 cagatctcaa atggagattc aacatttgta gatgtggtcg cagaaggtga aaaaaacgtt
    17281 ggtattaagt taacagcgaa gaaaaccggt gcttattatc gtagttatga gctgaagtta
    17341 cccaagccag gtcgggcata cagtgttcga gtagttcgta ttactgatga taacagcggt
    17401 cagtatcttt ataacgatac atgggtggac tcaatcggtg agatcgttga tacaccgatg
    17461 aactacccaa actctgcgtt ggtgggactg aaggttaatt ctgagcagtt tggcggctct
    17521 atgccttctc gttcctatct ggtgcgggga ttaaaaattc gcgtaccatc aaactacaac
    17581 gaggctagca acacttatga tggcgtatgg gatggctctt ttaagctgtt gtcatcttca
    17641 aaccctgcgt ggattctctt cgacttgctt accaattctc gatatgggct tggtcagtat
    17701 gtgtcggaat ctatgattga tctcggccaa ctgtaccaga tcggtcgata ttgtgacgaa
    17761 gaggttgatg atggctttgg tggtaaagag aaacgctttg caatcaatac gcagatcacc
    17821 agtagacagg atgcataccg actaattcag gatattgccg gtgcattccg cggcatggtg
    17881 ttttgggctg gtggcatggt taacatcatg caggatagcc catcagatcc agtaatgatg
    17941 tttaccaact cgaacgtcaa agatggattg tttacctata aaggttctgc gcgtaaagat
    18001 cgcccatccg ttgcgctcgt aacctacaac aacaaggagg acggttataa gcaaaacatt
    18061 gagtacgttg aagatcagga cgcaatgcgc cgttatggtg agcgtaaaac agaagtcgta
    18121 gcatttggat gtacaagccg aggccaggct caccgagttg gtttgtggct tttgtatacc
    18181 gccagaatgg agtcggatgt aattacattt actgccggtt tagacgcctc atttctgatg
    18241 cccggtgaaa ccgttctgat tcagaacaaa tatcgtgcag gtaaacgtaa ctccggtcga
    18301 attgtggagt ttaccaaaaa cagcatcact ctcgatgcgc ctgtgtcgtt agctaaaagc
    18361 ggcagcttta ttcggatatt gaatcaggaa ggcaaaatcg ttgaacgcga tgttcttgaa
    18421 actggcgaaa acataacaaa ggttacgttt tcaaaagctc tgtcgtcagc ggaaacgcct
    18481 gttttgaacg gtgtatggac aatcacagaa ccagatctcg aacctatgcg cgttcgcatc
    18541 gttaacatcg ctcaggggga aacgtcgggt agctttgaca tcaccgctgt tgagaacaat
    18601 ccgtctaaat atgaggcaat cgacaatggt gcaacgctta tcccgcagaa tacgacggta
    18661 ttggacccga cttactccaa gccgtctaat ttgcaaatca ccgaagggac ttatctctca
    18721 agcccaggca acctgtcagt aaaactgact gcaacatggg aagggaaatc tccagagtat
    18781 tggatcagtt ggcgacgttc tgatgaaaac aatgtatcga actggcaatc ggcacgtgta
    18841 accgaagagc aatacgaaat cgttaatgtc gcggagaatg gacgctacga cttccaactg
    18901 tatgcggttt cattcaacgg taaaaaaaca gagattatca gtaccgttta tcaggtgctg
    18961 ggcacaatga caccgccgga tgcaccaacg tcattaacgg ccgttggaga ctatcgtaat
    19021 gtgatactga attgggttaa tccagattcg gtagaccttg atcacattaa tgtttacgca
    19081 tcccagacca acaatctgga tacggcgaaa ctgatcgcag agtctgcaag caccaccttt
    19141 acacattctg gtcttgggga tagtgaaact tggtattact gggttagatc agcaaataag
    19201 cgtggcatgt tgagtcgccc aaactcaaat cttggtacag aggccacaac gcgagatgtt
    19261 ctttcatttt tagccaataa aattacctcg tcagaacttg gccaggcttt aaatgaggaa
    19321 attgacagta aggcttctca acaagctgtt gatgaaatca ataatcatat cgatcagagc
    19381 gtcgaatctc tggaaggtga aatttccgca ttaaataagg agctaacgag tagtatcgat
    19441 gagttcaaaa catcgtttac tgaacgcagc gagacactgg agaatgcgca aagcgagctt
    19501 agaacggaag tttctagcac tcgggaaaaa gtagatgatg cattacgaca agttgaaaat
    19561 tcaaatgcag cacttattga actacgtaat acagtatcaa aacaaggtga ttctgttgct
    19621 ggtgtcgtag aagcagcaaa tgcggcactt gaaaatgcat catctttaat tgccgaagag
    19681 cgtaaagttc gtgcacaagc agatctagcg aatgctaaaa agatagaaac gatgagatca
    19741 tcggttgatg aaagtgttgc ggctgtagaa gaaatgcgga gaacggttgc tgaggtagga
    19801 agagtaagtt cagaaatcac aactaatctt gaagccattt ctaagacaaa tatcgatctt
    19861 gcgttgcggc aggacgaaga tcagcacaga cagctggtaa ataacgcgaa aattgccacg
    19921 actcaaaaga ttcttgtcga tgatgtgtca gcaatggcgt ctaaggttga agagatacga
    19981 gccgagattg gtgacaacat tcgagcatct attctcgaag aagcgacagc cagaactgat
    20041 ggtgataatg cgttagccaa tcgtgtgaca caattacaga gtaaatttga aggtgatatc
    20101 agtgccgcaa taaggaccga acaatctgca agagcatcgg ccaatgagac tctttcaaag
    20161 cagatcacac aactggagtc taaagtaaat actgatattt caaccgctat tagagaagag
    20221 caacaaacaa gagcaacgca agatgaagca ttatcaaggc aaataactca acttcaaaca
    20281 aaagttaata acgatatatc tgctgctatt aaaagtgagc aacaagcgcg agttagcggc
    20341 gattcagcgt tatccacaca aattaatcaa ttgcaggcaa aagtagatga tgacatatca
    20401 gctgctatta atgaggaaag agaagccaga gcatcaggcg atagtgcact atcaagagaa
    20461 gttagtagtt taaaaacaaa aacaggtaaa gacattgcag cggcggtagc agttgaaacc
    20521 aaagcaagaa ctgacgctaa cgatgcatta tccagacaaa tttcttctct tactacgagg
    20581 gctaataatc ttcagtcctc gatagagcga gaatcgacat ctcgtacaag tcaggattct
    20641 gccattgcaa aagacttggc aagttttaaa acaaaaactg ctaaggatat aagtgcagct
    20701 gttgcggttg aaactcaagc aagagctgcc gctgataccg cattgacgtc gcagattaac
    20761 tcactaaaga cacaaacagg taaagatata aaggctgctg tagcagaaga gacgaaagcc
    20821 agaactgatg ctgatgcagc gctttcgaca agggtaacta atctccagtc gcaaacaaca
    20881 aattatatca atgcagcgat tacgtcagag tcaaaggcca gaacaagtgc agacaacgct
    20941 ctaggaaaac gaattgatac actgaaagcg tctgtagatg gaaatgttgc cgttattcag
    21001 cagcaggctt ctgctatagc tgacgctaat agaaaagtat caacctcgta cacattgaag
    21061 atggaaactt caacgagtgg tgggcagaag tatgtagcgg gcattgcgct gggtattgat
    21121 acaacggggc tttctcagtt tttggtgcgg gcagatcgat ttggtttggt caacaatgtt
    21181 aatgggaaga tcacaacgcc atttgttatc gaaaacagcg ttgcgtatat gaacggtgct
    21241 tatatcaaag atggcactat cacaaacgca aaggtaggtg acttacaatc aacaaacttc
    21301 gtaagaggac gagccggatg gagatttggc aaaaacggaa cgcttgagat taacggtaac
    21361 agtggaggaa atgggagatt agttatcaat gggcagagaa tcgacgttta cgacgacaat
    21421 aatgtactca gggtaagaat tggtcgactt tgattatgga aaaaaatatt tttatcgata
    21481 ataataggta gttacatact taatggtggg caaggatagc ccacctgatt aaaggagcag
    21541 attcaacatg tggtacaggg aaggtactat cacatttaca caaggcagca atactctggt
    21601 tggtgctggg acagcatgga acgttacggc caatggtgtg ttgcctggca tgattgtcgt
    21661 cgcacctgat aataaattgt atgaaattaa aagcgtaaca aatgacacaa ccttaacgtt
    21721 ggttgaaaac tatactggtg aaacgcaaag tgatatacca tgtaggatca tcactaccta
    21781 cgaaggagat ttgacacagt ttagtgcgag atttacggct cttttatcac gaatgtctgg
    21841 tgatgcaaaa acagttcgaa attggttaac tgccgtagat gaagttactc ttgagcgaga
    21901 agatggcact aatgttaatg taaaacctct ttctaagatt atcgcggagc ataataagca
    21961 cgtagaatgg tatgaaaata atactaacgc aattaattcc gcaggagata aagcaaaaca
    22021 ggctgcggcc agtgcgtcgg ctgcggcgaa gagtgcgaat ctagcaagca gtaaggctac
    22081 tcaggcatct aatagtgcgt tggatgctac agcgtctaaa aatgcagcaa ataaaagtgc
    22141 atcaagtgct aaagcatcag aaacaagtgc tttagaggcg aagaacgcag ctgcaatttc
    22201 tgcatcaaca gcttccacta aggcaacaga agccacaagt tcggctaatg ctgcgttggt
    22261 ctctaaagag gctgcaaaat tatctgaaat gaatgctgct tctggtgctg gcagtgcatc
    22321 aacttctgca aatcaggcta gagagtcggc aaggtctgcg aagacatccg ctaatcaggc
    22381 ttcaacttat gcgtctgctg ctagagcttc gaaggatgaa gctagccgaa agtctgctga
    22441 agcctcaact agtgctagta acgcatcatc gaaatcagct gaatctgcgg ctaaagcaga
    22501 agaagcaaag caaagccaaa catcagcact tgcatcggca gagcgtgcag agatagctgc
    22561 tactagagca gaaagagctt tgtcagcgat atcattacat gatgccagtt tgactatgaa
    22621 gggtattgtc aaattaagca gtgagacgag cagttcgtca gaagaatttg cagcaacacc
    22681 aaaagcagta aaggctgcgt ttgatcttgc taaatcaaaa tatactgcaa atgacgcgac
    22741 aacttcacaa aaagggctgg ttcaattaag tagcgataca aataacgcct ctgaatcatt
    22801 ggccgcaaca acaaaagcgg ttaaaagtgt aaaggatctt gctgatagaa aagcagcaat
    22861 caacagccca gcatttactg gcacaccaac ggcaccaacg gcgactcaag gcactaataa
    22921 tacgcaaatt gccacaactg cttatgtaag agaagcgatc tcaggtcttg taggatcatc
    22981 acctgaagcg cttgatactc ttaatgagtt ggcaagggct ttgggtgatg atcccgattt
    23041 tgccacaaca atgacgaaca agttggccac aaaacaacct cttgatgcta cgctgactgc
    23101 actggctgga ctaacaacag ccgctaataa acttccttat tttacaggga atgatacagc
    23161 tggattagca acaataactt ctgttggtcg agatattctt tctaaagcaa gtgtacaggc
    23221 gattattcag tatcttggat tgaaggaaaa tggaacaagt ggcgataaaa tacctttatt
    23281 gagtacagcc aatacatgga gtgcaaagca aacatttagt aatggtcttg caggctcact
    23341 taacggaaat tccgataccg caacaaaatt aaaagcagca cgaaaaattg gcggagtacc
    23401 gtttgatggt tcatcagata taaatctacc aggtgtaaac tcaacaggaa ctcagagtac
    23461 tagtggaaac gctgcctctg ccactaagtt agcaacacct cgttcaattg gtggaatgtt
    23521 atttgacggc actgcaaata ttgacttacc aggcgttaac aaggttggaa atcagagtac
    23581 aacaggtaat gcagggagcg caacgagact gcaaacggca agaacaatag gtggggtatc
    23641 gtttaatggc actgcaaata ttgacttacc tggtgtcaat aaagctggaa atcagaacac
    23701 gtcaggtaat gctgcaacgg cgacaaaact tgcaacggca cgaaaaataa atggggtcag
    23761 ttttgatggc tcaaagaata taagcatcac gccaaaacaa ctaggtgcag caaaatatgt
    23821 tgctaatctt gctacgggga atactcaaga gtggacaact gcacaatttg tcgcatggtt
    23881 aaattcacaa ggggcatttg atgcaactca gtggagcaca cgttgtagct ggtcttatgg
    23941 tagtaactgt tatataggaa atgcatcaac gggatgtgga gttatacctc ttgctggttc
    24001 tgttattgag gtgttttcaa ccggtacaag taattacact atacgcatta caacaccttc
    24061 aacatctgga ggtgcatctg gtgctgttct taatagtgag tttatttata catacaacgg
    24121 cgataactat agcccaacat ggcgtcgtaa ttacagttct caaaataaac caacggcttc
    24181 agagataggc gcccttgcct caaatggaaa tgcagtttcg gctacgaaac tgaaaacagc
    24241 gagaaatata gggggggtat cgtttgatgg tacagcgaat atagatttac caggcgttaa
    24301 caaagctgga aatcagaaca catcaggtaa tgctgcaaca gctactaaat taaaaacatc
    24361 aagaacaata ggtggagtat catttgatgg tagcgcaaac attaatcttc caggtgtgaa
    24421 tacgtcagga aatcagaata catctggtaa cgcagcatca gcaactaagc tacaaacagc
    24481 acgtactata aatggagtaa gtttcgacgg aacaaaaaat attagtataa gtgccgctca
    24541 aattggtgct ttgcctatcg gtgggggtac gataacaggt aatttgactg taaatggaaa
    24601 tggctcattt aatgatgttc agatccgctc agataagcgt aacaagcgta atctggtaaa
    24661 gctagataat gcgttagatc gtttggaggc acttactggt tatctttatg aaatccaact
    24721 ctctgatgat agttggaaaa cgtcagttgg tttaattgct caggatgcac aaaaagcatt
    24781 gtctgaactg gtaactgaag acacagacgt tatatctggt gaaaaacgtc tgtgtcttaa
    24841 ctacaacggc ataattgcat tgttagtcga gggctttaaa acacttcgtc atgagattaa
    24901 agaacttcgg gagaagtaaa cgacagctgt tggagtttct ggtttctact gaatttaaat
    24961 tgtgggggtg atactcaccc cacgcattca aaaggagggg taaaatgggg gtagcgtcgg
    25021 gatgggtcgg ttcttcggct aagagcgaaa cgggcgaaca atggatgagc gctgcgggta
    25081 ctaagttagg attgggtaaa ccttttatga tgagtcaaat ggtgggacga tctatgggct
    25141 gtaaaattgc aaccgcttac tataaaggga atcctcctga taaagtcgaa aactgggggg
    25201 ctgtaggttc agattggcca ttggctgaaa agaaccaagg tacaattgct ggtgcttcga
    25261 gctgtggttt aggtcgttta gtggggatcg tgcttacgca agctcactac gttccaggtt
    25321 taccgcccac tgggggttta tacatcgcag ggggaagggc aagcaatatt acagtaacag
    25381 ttggtggagt ctcacaagtt tttacttaca atagtgttat taacggtttt cactattact
    25441 ggcaaggaac acctagttcg gccttt
//
LOCUS       DAZOXR010000049        25104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZOXR010000049 DAZOXR010000000
VERSION     DAZOXR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.049"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            194..382
                     /gene="bcsR"
                     /locus_tag="ACHUNN_001701"
     CDS             194..382
                     /gene="bcsR"
                     /locus_tag="ACHUNN_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0576104.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHUNN_001702"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHUNN_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0576105.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="ACHUNN_001703"
     CDS             1143..1307
                     /locus_tag="ACHUNN_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR0576106.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHUNN_001704"
                     /pseudo
     CDS             1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHUNN_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHUNN_001705"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHUNN_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0576107.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHUNN_001706"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHUNN_001706"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0576108.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHUNN_001707"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHUNN_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHUNN_001708"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHUNN_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0576109.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="ACHUNN_001709"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="ACHUNN_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0576110.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="ACHUNN_001710"
     CDS             14457..15953
                     /locus_tag="ACHUNN_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0576111.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHUNN_001711"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHUNN_001711"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0576112.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHUNN_001712"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHUNN_001712"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0576113.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="ACHUNN_001713"
     CDS             18047..20107
                     /locus_tag="ACHUNN_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0576114.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="ACHUNN_001714"
     CDS             complement(20289..21611)
                     /locus_tag="ACHUNN_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0576115.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHUNN_001715"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHUNN_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0576116.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="ACHUNN_001716"
     CDS             complement(23084..23983)
                     /locus_tag="ACHUNN_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0576117.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="ACHUNN_001717"
     CDS             24503..>25104
                     /locus_tag="ACHUNN_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0576118.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6365 c         6990 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc actgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAZOXR010000050        24379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZOXR010000050 DAZOXR010000000
VERSION     DAZOXR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.050"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            266..403
                     /locus_tag="ACHUNN_001718"
                     /pseudo
     CDS             266..403
                     /locus_tag="ACHUNN_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(511..1746)
                     /locus_tag="ACHUNN_001719"
     CDS             complement(511..1746)
                     /locus_tag="ACHUNN_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0576119.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1893..1969)
                     /locus_tag="ACHUNN_001720"
     tRNA            complement(1893..1969)
                     /locus_tag="ACHUNN_001720"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1933..1935),aa:Pro,seq:tgg)
     gene            complement(2012..2098)
                     /locus_tag="ACHUNN_001721"
     tRNA            complement(2012..2098)
                     /locus_tag="ACHUNN_001721"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2062..2064),aa:Leu,seq:cag)
     gene            complement(2119..2194)
                     /locus_tag="ACHUNN_001722"
     tRNA            complement(2119..2194)
                     /locus_tag="ACHUNN_001722"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2159..2161),aa:His,seq:gtg)
     gene            complement(2253..2329)
                     /locus_tag="ACHUNN_001723"
     tRNA            complement(2253..2329)
                     /locus_tag="ACHUNN_001723"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2293..2295),aa:Arg,seq:ccg)
     gene            complement(2430..3815)
                     /locus_tag="ACHUNN_001724"
     CDS             complement(2430..3815)
                     /locus_tag="ACHUNN_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0576120.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(4006..4746)
                     /gene="wecG"
                     /locus_tag="ACHUNN_001725"
     CDS             complement(4006..4746)
                     /gene="wecG"
                     /locus_tag="ACHUNN_001725"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0576121.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4749..6101)
                     /gene="wzyE"
                     /locus_tag="ACHUNN_001726"
     CDS             complement(4749..6101)
                     /gene="wzyE"
                     /locus_tag="ACHUNN_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0576122.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6098..7177)
                     /gene="rffT"
                     /locus_tag="ACHUNN_001727"
                     /gene_synonym="wecF"
     CDS             complement(6098..7177)
                     /gene="rffT"
                     /locus_tag="ACHUNN_001727"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0576123.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7174..8424)
                     /gene="wzxE"
                     /locus_tag="ACHUNN_001728"
     CDS             complement(7174..8424)
                     /gene="wzxE"
                     /locus_tag="ACHUNN_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0576124.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8426..9556)
                     /gene="rffA"
                     /locus_tag="ACHUNN_001729"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8426..9556)
                     /gene="rffA"
                     /locus_tag="ACHUNN_001729"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0576125.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9561..10235)
                     /gene="rffC"
                     /locus_tag="ACHUNN_001730"
                     /gene_synonym="wecD"
     CDS             complement(9561..10235)
                     /gene="rffC"
                     /locus_tag="ACHUNN_001730"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0576126.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10213..11094)
                     /gene="rfbA"
                     /locus_tag="ACHUNN_001731"
     CDS             complement(10213..11094)
                     /gene="rfbA"
                     /locus_tag="ACHUNN_001731"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0576127.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11113..12180)
                     /gene="rffG"
                     /locus_tag="ACHUNN_001732"
     CDS             complement(11113..12180)
                     /gene="rffG"
                     /locus_tag="ACHUNN_001732"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0576128.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12180..13442)
                     /gene="wecC"
                     /locus_tag="ACHUNN_001733"
     CDS             complement(12180..13442)
                     /gene="wecC"
                     /locus_tag="ACHUNN_001733"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0576129.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13439..14569)
                     /gene="wecB"
                     /locus_tag="ACHUNN_001734"
     CDS             complement(13439..14569)
                     /gene="wecB"
                     /locus_tag="ACHUNN_001734"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0576130.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14625..15671)
                     /gene="wzzE"
                     /locus_tag="ACHUNN_001735"
     CDS             complement(14625..15671)
                     /gene="wzzE"
                     /locus_tag="ACHUNN_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0576131.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15683..16786)
                     /gene="wecA"
                     /locus_tag="ACHUNN_001736"
     CDS             complement(15683..16786)
                     /gene="wecA"
                     /locus_tag="ACHUNN_001736"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0576132.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17026..18285)
                     /gene="rho"
                     /locus_tag="ACHUNN_001737"
     CDS             complement(17026..18285)
                     /gene="rho"
                     /locus_tag="ACHUNN_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0576133.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18370..18471)
                     /gene="rhoL"
                     /locus_tag="ACHUNN_001738"
     CDS             complement(18370..18471)
                     /gene="rhoL"
                     /locus_tag="ACHUNN_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0576134.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18612..18941)
                     /gene="trxA"
                     /locus_tag="ACHUNN_001739"
     CDS             complement(18612..18941)
                     /gene="trxA"
                     /locus_tag="ACHUNN_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0576135.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19072..20337
                     /gene="rhlB"
                     /locus_tag="ACHUNN_001740"
     CDS             19072..20337
                     /gene="rhlB"
                     /locus_tag="ACHUNN_001740"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0576136.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20471..21955
                     /gene="gppA"
                     /locus_tag="ACHUNN_001741"
     CDS             20471..21955
                     /gene="gppA"
                     /locus_tag="ACHUNN_001741"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0576137.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(22002..24023)
                     /gene="rep"
                     /locus_tag="ACHUNN_001742"
     CDS             complement(22002..24023)
                     /gene="rep"
                     /locus_tag="ACHUNN_001742"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0576138.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24240..>24379
                     /locus_tag="ACHUNN_001743"
     CDS             24240..>24379
                     /locus_tag="ACHUNN_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576139.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6144 a         6672 c         6009 g         5554 t
ORIGIN      
        1 tcaattaata acacatatac taattgccgt taaaactaaa aaagcattaa taatcaacgc
       61 aatataataa acccgcctta catatcaact gcgccagagg taggattgaa aacgctctcc
      121 tgattttcca attcattttc tggataaata aataatttat ttttgtcact attatttatg
      181 taatcatcct gtcagggaga gggatctcaa ttatcaatgc ttaattacgt catcattttg
      241 atgacatgaa accggagcat tttgcatgga attttcgttt tcacccaaac ttcttgttgt
      301 tgctgtcgcc gccgctcttc ctctcatggc cagcgcagca gataccccgt caactgccac
      361 cgcacgcaaa ggctttgccg gatacgatca cccaaaccag tatccacaga acgctgcaat
      421 ttattgaatt tgcacgatca tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca
      481 accgcaggcg gccacaaaat ggccgctttt ttacttattc accaccagca aatgtgcctg
      541 cataatgtca ctggccgggc gaccgtgcgc cagcaaatca gccattgctt taagatatgg
      601 cggtagatgg cggaaataac gctgataccc ggcacacaaa taattcagcc ccggtttgcc
      661 gctggcatcg agcatgaagc ggtgtttcgg gcagcctccc cagcacgctt ttaacacgtt
      721 acaactgcga cactgcgccg gtaattgctt aaatttatct tcaccaaaca cctgctgttg
      781 cggggaatcg atcatttctg caattgtttg ctgatgcata ttccccagcc gatattgcgg
      841 ataaacatag tgatcgcagg cgtaaacgtc gccgttgtgc tcaacaatca ccgagcgccc
      901 acaggttggc tgatgatggc aaaccgcacc cggcgcaccg acaaaattgg caaacgccca
      961 ttcgatattc atcacgaaaa tcttgccgac gtcgcgtttg atccagtggt cgaatatcgc
     1021 caccagaaat tcaccaaatt catcggggcg caccgaccat tccgtcagtt ccccctgaat
     1081 atcaccaggc gcatgcaatt tcagtccatc gcgggcagct gtttcatcag ccagacgctc
     1141 gaccaccgga ataaactgga tgaattcgac tcccgcatcg cacaaaaaat cataaacctg
     1201 caacggttgc tgcgcgctgg tgcggttgac gcagaccagc acgttatagt cgacatgatg
     1261 tttttgcagc agcgtcaggg cacgcatcac cagcttatgc gttggtctgc cacctttggt
     1321 cacgcgatat tgattgtgga tctcagccgg accgtccagc gataacccaa caagaaaatg
     1381 attttctgcc agaaatgcac accatttatc atcgagcagc acgccgttag tctggaagct
     1441 gttacttatc ttcctgccag caccgtattt cgcctgtaac tttacggcac ggcggtaaaa
     1501 atccagcccg agtaacgttg gttcgccgcc ctgccaggta aaagccactt cgttttgcgg
     1561 ttcgctggca gcgatatagt gacggacata cgcttccagc gtgtcatcgt ccatatgcgt
     1621 gactggcttt tcgcggtaaa gggattgttt ttcgagataa aaacagtagt cacagttcag
     1681 attacaatcg gaaccactcg gtttcgccat cacatgaaaa gcacgcgttg gaacctgttg
     1741 cagcatggtc actccttgcc tgaagtcatc cttcaatacg ttaagggcga aatcataccg
     1801 tggggcaatg agttattacg ggcgtttatt gcgaagggag ggtgacgaaa tgcacagaaa
     1861 acaaaaaacc ccgccgaagc ggggttcaaa attggtcggc gagagaggat tcgaacctcc
     1921 gacccactgg tcccaaacca gttgcgctac caagctgcgc tactcgccga aatactgctt
     1981 tttgaatttt tagttcaatt ctttaaagtc gtggtgcgag gggggggact tgaaccccca
     2041 cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc accttcgcat
     2101 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
     2161 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
     2221 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
     2281 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
     2341 gcggggggat aatacggact tcccacccct ctgtctagtc cctttttaaa taaattgcgc
     2401 gtttggttac ggtttgtgcg ttttgctgct tattcctcca gtttatgcgc tttcccgtgg
     2461 cgattaaggc caaaaacttt ataaatcgcc gtcaccgcca gcataaagat gatgccaaca
     2521 aacagcgaca tacgcgtatc ttcattaaag tacatgccga tcaaaacgca aatcaggaat
     2581 gccattgtta cgtagttggc ccacgggaac aggattgagc ggaacggatg gctggcaatc
     2641 gccgctttat gtgcacgccg aaaacgcagc tggcttatca atatcacaaa ccatggcacc
     2701 atccccggaa gcacgctggc actgtagacg tagacaaaca cacgctgcgg attgggaatg
     2761 atgtagttca ggcatgagcc aattagcaga atagcaatag atactgccac acccgcaact
     2821 ggtacgccgt gacgggaaac tttcgccatc gccgccggta actgacggtt tttcgccagt
     2881 gcgtagagca tacgtccgca actgtacatg ccgctgttac agccagagag cgcagccgtc
     2941 agcaccacaa agttgataat gcccgccgct gcggtaatac cgattttggc aaaagtcagt
     3001 acgaacgggc tgccgttgct gccaatttca ttccacggga agatggtgac gataacgaaa
     3061 atcgcgccta cgtagaaaat caggatccgc cacagcactt tgcctacagc actgcgcagc
     3121 gtcacctgcg gattcttcgc ttcaccggca gtaatgccaa tcagctccac gccctggtag
     3181 gacgccacca caatacacag cgcggtcagg aaccctttcc agccacccgc aaagaaaccg
     3241 ccatgctctg tgagattgct aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa
     3301 atcacgccca ggccaatgac aatcatcacg ataatcgtgg tgactttgat catcgcgaac
     3361 cagaactcga tttcgccgta caaccgcacc gccgccagat tcgccaacgc caccagcgcc
     3421 accgcgatca atgcaggtat ccactgcgcc atctccggga accagaactg gacataaacg
     3481 ccaatggcgg tgatttctga gatccccacc gccatccaca taaaccagta agaccaggcg
     3541 gtgagataac caaaaaacgg gctcatataa cgatgcgcat aaacggcgaa cgaaccggta
     3601 acaggttcaa ggaacaacat ttcgcccatt gaacgcatga tgaaaaagac gaacagcccg
     3661 gcgatgatat aggccaacaa tacggatggc ccggcccatt tcagggtact ggcggccccc
     3721 ataaacaggc cgacgccaat agtgcccccc agggcgatga gttcgatatg tcgagcttcc
     3781 agcccacgct gtagctctgg tttgttatct gccataaatc ctcgtgttgt gtttgcatgc
     3841 tttccggtgt taccggttat cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt
     3901 caggagaaat ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga
     3961 caggcgacgg agtgaccact ccgtcgcttt acaaagagag gaaaatcaga ggttgccggt
     4021 gtagtgccag cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga
     4081 aagcaggcgg tagagccact ccagtcccag cgtttgccag attttcggtg cgcgttttac
     4141 gtgaccggtg aaaacatcgt aagtcccgcc aacgcccata tacagcgcat ctggatgcac
     4201 cagacggcag tcgcgcatga agatctcctg ctttggcgat cccatcgcaa cggtgacgat
     4261 ttgcgctccg ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata
     4321 accatcctga ctgccaacga tattcacatt ccattggttg cgcagtttag cttcagtttg
     4381 cgccagcact tcaggtttac cgcccacaag aaataccggc gtcccttctt tgcctgcgcg
     4441 cgccatcagc tcttcccaga gatcggcacc ggcaacgcgg gaaacctgcg cctgcgggta
     4501 ctttttacgt actgaacgta caacgctgat gccatccgca tatttaaatt cggcagcgtt
     4561 aattaactcc ctgacctcgg cgttatcttc aatggtcagc attttttcag cattaatggc
     4621 aaccagcgtt ccctgcttaa gctgcccgtc agcaaacaga taatcgaggg cgtgctgcat
     4681 atcacgccaa ccaatcaact gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt
     4741 attcattgtt atccttcaac ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt
     4801 ccggcgcttt caaaaagcca gtacagcagt tttgcgatca tcagacatgc gccgaagacc
     4861 acgataaaaa agactacgcg tgagacaaac gaatccagcc cttcacgcgc cagcacgatc
     4921 atattgaaga tcgccccaaa gcagaaactg tgcaatatcg cagccttata gcgattaggc
     4981 tcgcggttgc ccagctcata cagccagtcg aaccatttga tgatcagacc aaccacgatc
     5041 gccccgagcg ggatgaacaa cgcgccgccc atcaccacca gtgagcctat aagcgtaggc
     5101 gagatcgcca gtccggagtg gttattcagc acttcccagg taaagtagtt ggctgagttc
     5161 agcaccatac tcgggcgacc cggccacagc caggaaggga taaagacata gaaatcgcgg
     5221 acaattggag ccaggccctg gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga
     5281 ttctcccacg gggagaaggt gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg
     5341 ccgctcacat tcattccata gcgttttagc gccagccaga acatgccaac aatccccagc
     5401 acgcccgccg ccgccagcat ccacaacgaa atccagccgc gaataatgcc aataaacagg
     5461 aagatagcga atgcgatgat gatattggcg cgagtgccgc cgacaatcat ataagtcagc
     5521 aagccaaagg cgaccgtgct gacgaggaaa aacagccacg ctttgctgtc ctggcgcaga
     5581 aagtagacca ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg
     5641 gagacttcac tggagaagat ctgactgtag gagttaagcc ggaacagcaa aaagccgttg
     5701 tgcatgaaga agatgccgac gcttaccagc gcgataccca tcaggatcac ccacgtaaga
     5761 ttggtctcca cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg
     5821 cgtaggcggg ttttgtaggt gacatagtaa accgcgtaga agcagcccgc agaaagcaac
     5881 gcctgcaaca agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg
     5941 gtcagcggga agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag
     6001 ttaaagcgca cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc
     6061 agccagacca caaacaggcc actgaattgc agcagactca tgcgacctcc ccggcggcaa
     6121 tagccaacgc ccgctgccag ccttgtagat agttagggct aaagaaggca atggtgtttt
     6181 tatccaccga cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg
     6241 tagtaaacag caccggcaaa tgctgttccg tcatatcctg ccagaacgga ttttcccggt
     6301 taagcacaca aggaatgccc gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc
     6361 gggcaaaaat aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt
     6421 ccagtttttc gctcaaaatt tgcagatttt cttcgctgaa taactccagc cccgcctgac
     6481 gaacttcctc aatgtacgct tcgttattag gcggatatcc catcggcacc accactttta
     6541 ccgtatcgcc aaattgctga tgaacggcgc gcaaggcagc aatatggtca ttgctgcggt
     6601 caccggagtt ccccaccaga atggtcattt tcccttcacg ttgccgatca ttcgccatcg
     6661 tattgagaga agggtccatc cgcgtcggga agtacagcag ttcgccccgc acctttggat
     6721 gcgttttggc aaaaaagctc aaatcaccgc gggtggcaaa tacacagccg actcgctttt
     6781 gcgccaggcg acgtagcggg taaaaaagct tatatctcaa gccactggaa agctcataca
     6841 ggtctgcccc ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag
     6901 ccagccacag tgtgggattg aactgaccgt ggaaaaaaaa tcgctgctga cggttagctt
     6961 ttgctttcgc gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa
     7021 gcgccggaca gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt
     7081 gctcgctcgt cgcggccagc gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag
     7141 ggatatccga tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa
     7201 cacgccacaa caaagagaaa aatagacgat ataagttgcc atatatgcct gcgccgcgcc
     7261 cagtgcacca tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct
     7321 gacttccgcc agaatataaa accgcagtga cgctttggcg atcaccagat aaccaaagac
     7381 ataagcgccc acttttaaca catcacccac taactgccag gcaaagagat cgcgcatagc
     7441 ggtaaattta ttcgacaaca gcaaccagat agcaaaatca cgcagcaacc agacggtaaa
     7501 actcgccgcc gccactgccg gtaagacgaa tttcagcgat ttaaccactt cccgggtgat
     7561 atcgcgcttt tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt
     7621 aataaattgc aggtaggcat cggaaatact gctcacccct tgccagatcc ccacttcatc
     7681 ccagctatac tgcgccgcca gcagtttacg catcatgatg taagcaacag gcaaggtcac
     7741 cgacgtaatc aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca
     7801 gctgggtttc agatagctta acgggatgac accacgtttg atcaacatga tggcggcagg
     7861 aatcaccacc agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgcccaa
     7921 acggtaactg acgtagtacg cgagaacgcc aatcaagctg ccgacaatca gcgataacgc
     7981 attacctgcg gcatcgcgaa agcctttcat cagcgccagt aacaggttgc cccaggcgat
     8041 ccccatttgc accagcgcca ctaaacgcac cagcccctga tagtcggtat taccaaacag
     8101 tccctggctg attggcgcag ctgccagcac aaaaaccagc gccatcagcg tagagaaacc
     8161 aagtaccatc gctgatgaag tgccgaccac gcggcgcagc tgttgcggat tatcatggta
     8221 ctgggcaacg tatttggtta caccgttaaa gatgccagct ccggcaagca cgccgagcac
     8281 ggtaatcaac tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacacagc
     8341 cagcaactta ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca
     8401 caaggacgct tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg
     8461 tacgctgatt gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcgctct
     8521 ctttggtggt gtagcgatct tcaccgtgga actcaccaaa gcgttccccc gcagggcaac
     8581 cgtgcagcgg aatgtaatgg aacaccgcca tgatttccgc ttctttcaga aagttaatca
     8641 acgcgctccg gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc
     8701 agccatcggg aatcgacggc agctcgatac gcccggcttt cgccagaggc gctaacgcat
     8761 cgtagtagtt ttgccacagc gccagacgtt gctggttgat acgatccgct gcttccagtt
     8821 gcgcccacag gtacgcagct tgcagatcgg acatcaaata gctggagcca atatcgcgcc
     8881 aggtatattt atcgacctga ccacggaaga actggctgcg gttagtgccc ttttcacgga
     8941 tgatctcggc tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg ccttcaccac
     9001 ccgccgtata gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca
     9061 gtgcacgccc tttgtaagtg gacatcacgc cctgagcggc atcttctacc acaaacagat
     9121 tatgcttttt cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga
     9181 ccggcacgat aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt tcgtcgatgt
     9241 tcatggtgtc cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc acaaaggcat
     9301 tggcggtgga gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga
     9361 gcagcagcgc cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc
     9421 tgccaaaacg ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca
     9481 gtttgccgct acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg
     9541 cgttaaatgg aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca
     9601 ccactttgta tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa
     9661 gttgttttac cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctgca
     9721 ccgcgtccag ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca
     9781 taaccgcgaa taccgccgga cgccgcacgt aaaatcagac attgatgatc aaaggtgccg
     9841 cgcacggcat tttcaatcca ctgtgcataa aagcgtccgc tggcgtcagg cgcataccac
     9901 ggcgcacgaa aacggctttg cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata
     9961 tcggtctctt gtgccactac agcaccacta tcactgacat tgttcacggg tagtgccaaa
    10021 tcaacttcac cttctaccag ggagaatccc agctgttgca gggcatccag ttcacccgta
    10081 tttgatgccg caattttggc ctgcacccgt gaccacggcg ctaacacgtc tggcgtcagg
    10141 agctgtgctt cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc
    10201 caggttagtg gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc
    10261 gtagccagtt ttcgctaatg aactggcagc acgcttcaca ccctcgtcat cgagccagcc
    10321 gttacgccag gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttctactgt
    10381 ctgcacaaag gtactggctt caatcaggct gtcgtgagtg ccagtgtcca gccaggcaaa
    10441 tccgcgcccg agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga
    10501 ggtaatctcc agttcaccac gctccgacgg cttcacctgc tttgcgtact ccacgacttt
    10561 actgtcgtag aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc
    10621 ttccagcgag atagcgcgga aattgtcgtc aaactccacc acgccaaagc gttccgggtc
    10681 catcacctga tagccaaata ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt
    10741 cggactgaag ccctgaccga agaagatgtt atcgcccagc accagacaag aaggttcacc
    10801 gttgaggaag gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc
    10861 ggcgtattcc agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc
    10921 tttatcttcc ggcgtagtga tgatgagaat ttcgcggata tcggccaaca tcagcaccga
    10981 cagcggatag taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg
    11041 cgtaatcgga tgcaatcggg tgccggaacc gcccgccagg ataatacctt tcatgtttgc
    11101 ctccgctgaa aattagcctt tcagacctaa acgctcgccc tgatagctgc cgtcctgcac
    11161 ctgcttccac cagctttcat tagccagata ccactgcacc gttttacgca ttccactttc
    11221 aaaggtttcc tgtggcagcc agccaagttc acgggcaatt ttcgaagcat caatggcata
    11281 acgcagatca tgccccggac ggtcagcgac aaaggtgatc aagtcacgat agtgcaccac
    11341 gccgtgcggc ttgttcggag ccagttcttc cagcagctcg caaatggttt ccacaacatc
    11401 gagattctta cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt
    11461 cgccacgcaa tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg
    11521 cccgttgcca tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat
    11581 cagtttttcc ggaaagtggt aaggaccgta gttattcgag cagttggtga taagcgtcgg
    11641 cagaccgtag gtccgcagcc aggcgcgcac cagatggtcg ctgctggctt ttgacgcgga
    11701 ataagggctg ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag
    11761 gtcaccatat acttcgtcgg tggagatatg atgaaaacgg aacgctgatt ttttatcttc
    11821 cgtcagcgta ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt
    11881 ggtttcaata aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag
    11941 atgcatgaca cagtctggct gatgctcagt gaatacgcgt gccagttctg cccgatcgca
    12001 gatatcaact ttctcaaagg caaagcgctc gctttgcgcg accggtgcca gcgacatcag
    12061 gtttccggcg taggtcagct tatcgaccac taccaccgcg tcgctcgttt cgttgatgat
    12121 ataacgcacc agcgccgagc caataaaccc ggcaccacct gttatcagaa tttttctcat
    12181 cagcgccaga ctcctttggc atcgacgaca tactgctgat ggacattgtc gccattgata
    12241 actttaaact gactatgatc gaccagcatc accagcacgt ctgccgttgc cagcgcctcg
    12301 tcaagctgcg ccagagtaca aagcccggtc agttttttcg gcaactggtg gatgttaggc
    12361 tcaacaacca gagtttcgcc gctatgccac tgggcgatca gttcagcgat ttccatcgcc
    12421 gggctttcgc gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc
    12481 agttcactgg cgcgtttatc ggtagccgcc aggcaatcag ccaccgccgc tttcacctga
    12541 tcgataaccc agaacggttt gtgatcgttc acttcgcgcg cggtacggat aagccgcgcc
    12601 tgctgggggt tctgtgccac gataaaccac ggatcaacag caatgcagtg accgcccacg
    12661 ccagggccag gctgaagaat attaacgcga gggtgacgat tcgccaggcg aatcagttcc
    12721 cagacgttaa tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc
    12781 acatcgcgga agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg
    12841 acacactcac cttcgaggaa aattttgtac agttcgctgg cgcgggtcga acaaaccggc
    12901 gtcataccac caatcacgcg atcgttttta atcagctcga ccataacctg gcccggtaac
    12961 acgcgttccg ggcagtaagc aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa
    13021 gtgagatccg gacgcatctc tgctaaccat tctgccatct tctcggttga ccccaccggc
    13081 gaggtggatt caaggatcac cagcgcgcct tttttcagca ctggcgcaat ggagcgagca
    13141 gccgattcaa cgtaggtcat atctggctca tgatcgcctt taaacggcgt gggtacagca
    13201 atcagccagg catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg
    13261 gcagttttta ctacgctcgc caaatcaggc tcgacgatat ggatttcgcc acgattgatg
    13321 gtatcaaccg cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac
    13381 gctgctgctg taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc
    13441 atagtgatat ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac
    13501 catacgggtt atgggcgcgg ctcatagttt gatattcgtt ttcgtctttt aaaagacgcg
    13561 tcacttcctc gacaattcgc tgcttatccg tgcctaccag acgcaccgta cccgccgtca
    13621 ccgcttccgg acgctcagtg gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg
    13681 cttcttcctg aatgccgcct gagtcggtca aaatcagcca ggcgtggttc atcagccaga
    13741 caaacggtaa atactcctgg ggatcgatca gaatgacatt tttcacatgc cccagaatgc
    13801 gattgaccgg ctctctgacg ttcgggttga gatgcaccgg atagacaatc tggatgtcct
    13861 ggtgcgtggt ggcaatgtct gccaacgcgt ggcagatttc ttcaaagcca cgaccgaaac
    13921 tttcacgcct gtgaccggtc accagaatca tctttttatc ggggtcgata aacgggtaat
    13981 ttgccgccag ttctgaacgc agcgtatcgc tgctcatcac ctggtcacgc acccataaca
    14041 gtgcatcaat aactgtatta ccagtaatga agattcggct atccgcaacg ttttcacgca
    14101 gcaagttttg ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca
    14161 atgtacggtt agcctcttcc ggccacggcg aataaagatc gcccgtgcgc agaccagcct
    14221 caacgtgacc aacaggaata cgctgataaa acgccgccag gctggttgcc agcgtcgtcg
    14281 ttgtgtcgcc gtgaaccagc acgacgtctg gtttgaactc ggcaagaata ggttttagcc
    14341 cttccagaat ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat
    14401 cgtagtcagg tacaatggaa aagagtttca gcacctgatc gagcatctcc cgatgctgcg
    14461 cagtgacgca aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggcg
    14521 ccatcttgat ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct
    14581 tcgaataagc ggcgagcgcc tttgcgctca ccgcagcagt gttgctattt cgagcaacgg
    14641 cgggttaatg cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg
    14701 aaggcacgac gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag
    14761 gtctgaaaac gcggatccag ggttggacca acattcaggg tgtttaacat ggcccgattc
    14821 tgatcatagt cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg
    14881 agcattggac gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct
    14941 gtcgcactgc gcgaaatatt atgctgctca gcaattttca gcgcctgttc aatactgttc
    15001 atccggcggt cgtagatggc tttcgccacc tcttcctgac gcttcacctg tgctttcatc
    15061 tggatggtac gcgccgccca tgcgcctttc agctcatcat tcagatggct ggctgcacgc
    15121 tggctggcaa aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcagca
    15181 ataagcttca cgctgtcatt gaccgcgcgg gtaaagtctc cggggataaa ctggatgttg
    15241 ttaatcattt catccagcaa cgccgcatcg gctttgctgt tgcccaccat ccgctgtttg
    15301 taatagtcgg tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca
    15361 aactctttat aggcttcgtc catgaccgat ggttggtcgg cagaagccat gtttgaacgg
    15421 acatccaggt tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat attcaccgtt
    15481 ggacgatcgg taatcgccgt cgagctccac tcctgacgag caaaaaaagt atacgccagc
    15541 gcgattaacg caaatgccag ccccatgcca ataatccata gcttcccagc ccacaaggta
    15601 cgaaacaacc cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt
    15661 ggttgtgtca tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac
    15721 gcagtctgcg ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat
    15781 atccatagag gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat
    15841 gagaatattc tgccagcacg ccaatggaag cgagcagtgc cgcagcaagg gtaatcagca
    15901 caaacgcctg acgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt
    15961 caggagagaa tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca
    16021 ttagcggaat ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct
    16081 gggtcgtttc gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac
    16141 ccataaagac tttgtagcgg cgacccagga taccaaggtt aagcatgatg tatggcagga
    16201 tggcggcgat catcgcaaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa
    16261 tcataccgat tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat
    16321 caaccatgtt gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtaaccaa
    16381 acggtccgag caccatctcc caggagccaa agatataacc caggctactg agataaagtt
    16441 tgccgaacac catcataaca atgccaacag cggcctgtat ggtggcacgg atttttacgc
    16501 tgatatcaaa acggtcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat
    16561 agagagatgc atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt
    16621 aaaccgaaat ccccccaacg agaggtatca atccctggtg acgtttgcgg aagtttggtt
    16681 tatccactaa accgactttt tttgccacct tacgggcaaa aaacagaaac agtgtcgtga
    16741 ataaaaaaat actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca
    16801 gagaaaatta ttagcagaag tataaccacg aagaccttta ttcagaaggg aaagtccgtt
    16861 ccgaacccac cagtcctgtg caaaaattaa ccatttgata tgcaaattgc ataacacgcc
    16921 agcaaattcc aggaattttc ctaacatcgc cagcgcggca ttaagattac agatataaaa
    16981 gcaaaacgcc acgtaaacac gtggcgtttt tggcataaga caaatttatg agcgtttcat
    17041 catttcgaag aaatcgtcat tggtcttggt catggccagt ttattaatga ggaattccat
    17101 tgcatcgatt tcgcccatcg ggtgaatgat tttgcgcagg atccacattt tctgcagttc
    17161 ttcctgagta gtgagcagtt cttctttacg ggtaccggaa cggttgtagt cgatagccgg
    17221 gaagacgcgt ttttcagcga tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt
    17281 aaactcttcg tagataactt cgtccatttt agaaccggta tcgataagcg ccgtcgcgat
    17341 aatagtcagg ctgccgccct cttccacgtt acgcgccgca ccaaagaagc gtttcggacg
    17401 atgcagggcg ttggcatcca caccaccggt caacacttta cctgacgccg gaacaacggt
    17461 gttgtaagcg cgcgccagac gagtgatgga gtcgagcaga atgataacgt ctttcttgtg
    17521 ctcaaccagg cgtttggcct tctcgatcac catttccgca acctgaacgt ggcgagatgc
    17581 gggttcgtca aaggtagaag caacaacttc accttttacc agacgctgca tctcggttac
    17641 ttcttccgga cgttcgtcga tcagcagaac catcagcaca caatccgggt ggttgtaagc
    17701 aatgctctgg gcaatgttct gcagcagcat ggttttaccg gctttcggcg gtgccacaat
    17761 cagaccacgc tgaccacgac cgataggtga tgccagatcc agtacgcgag cagttaaatc
    17821 ttcagtagaa ccgttaccac gttccatacg cagacgagaa tttgcgtgca gcggggttaa
    17881 gttctcaaag aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac
    17941 tttcagcagc gcaaaatagc gttcaccttc tttcggcggg cgaatcttac cagagatggt
    18001 atcaccagtg cggaggttga aacggcggat ttggctaggg gaaacgtaga tgtcatcagg
    18061 accggcgagg taggagctgt ctgcggaacg gaggaaacca aatccatcct gcaatatctc
    18121 cagtacgcca tcaccaaaga tatcttcgcc actctttgcg tgctgcttca ggatggcaaa
    18181 aataatgtcc tgcttacgca tgcgagccag gttttccagc cccatatttt cgccgagagt
    18241 gatcagctca gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg tgtgagttct
    18301 taaacttggg gtaaatctcg aacttaatgt tgtgaatggt atggcaggat catccatgcc
    18361 tgtttacggt catcgactca tgtcttttcg ctgcctggtc acaggagagt acgcagaact
    18421 gaaacgacaa gacggattga gtgacgagcc agaaatctgt tcacttcgca tttaagataa
    18481 aacgggaagc gttggaaatt acaagattca aacttaataa ggtatgttta atacgaagtc
    18541 aacactaagt tagcatgact cacgccgggc gtccagtttt tagcgacggg gcacccgaac
    18601 atgaaattcc cttacgccag gttagcgtcg aggaactctt tcaactgacc tttagacagt
    18661 gcgcccactt tggttgccgc cacttcaccg tttttgaaca gcagcagagt cgggatacca
    18721 cggatgccat atttcggcgc agtgccaggg ttttgatcga tgttcagttt tgcaacggtc
    18781 agtttgccct gatattcgtc agcgatttca tccagaatcg gggcgatcat tttgcacgga
    18841 ccgcaccact ctgcccagaa atcgacgagg atcgccccgt ccgctttgag tacatccgtg
    18901 tcaaaactgt cgtcagtcag gtgaataatt ttatcgctca tatataactc cacaggaata
    18961 agcctggcgt gttggtttcg ttgttggtgt aacattaacc aactaaaggt tgactttatt
    19021 tcaccggata cgctttcgta aagcaatagt aagctgatat tctaccacac tatgagcaaa
    19081 acacatttaa cagaacagaa gttttccgac ttcgccctgc atccgaaggt tgtagaagcc
    19141 cttgaaaaaa aagggtttca taactgtacg cccattcagg cactggccct tccgctgacg
    19201 ctggcgggtc gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac gatggcgttt
    19261 cttacgtcaa cgtttcatta tcttctctct catcctgcga ttgccgatcg caaggtgaat
    19321 caaccgcgtg ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat ccatgccgac
    19381 gcagaaccgc tggcggaagc tactggcctg aagctgggtc tggcttacgg tggtgatggc
    19441 tacgacaaac agctgaaagt gctggaaagc ggcgttgaca ttctgattgg caccacgggt
    19501 cgtttaattg actacgccaa gcagaaccac attaacctcg gtgccattca ggtagtggtt
    19561 ctggatgaag ccgatcgcat gtacgatctg ggctttatta aagatatccg ctggctgttc
    19621 cgccgtatgc cgcctgcaaa ccagcgcctc aacatgctgt tctccgccac gctttcgtac
    19681 cgggtacgtg aactggcgtt cgagcagatg aacaatgccg aatatattga agtggaaccg
    19741 gaacagaaaa cgggccaccg tattaaagaa gagcttttct acccttctaa cgaagaaaaa
    19801 atgcgtttgc tgcaaacgct gatcgaagaa gagtggccag acagagcgat tattttcgcc
    19861 aataccaaac accgttgtga agagatctgg ggccacctgg cggcagatgg tcatcgtgtc
    19921 ggtttgttga caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc
    19981 cgtggcgatc tggatattct ggttgccacc gacgttgccg cgcgtggttt gcatattccg
    20041 gcagtgacgc acgtctttaa ctacgattta cccgatgact gtgaagatta cgttcaccgt
    20101 attggtcgta caggtcgcgc aggcgcaagc ggtcactcta tcagcctggc gtgtgaagag
    20161 tatgcattga atttgcctgc tattgaaacc tatattggtc actcaattcc ggtaagcaaa
    20221 tacaatccgg acgcattgat gaccgatctg ccaaaaccgc tgcgcctcac gcgcccacgt
    20281 acaggcaatg gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata
    20341 aaatgtcata ctcaaaatca ttcaaactgt atcaagatgg caagagaatg aatcccgatg
    20401 agcatactgt agtaagtgat tcaggtgaaa gaatgcagcc aacacagaga cagattgaag
    20461 gatgaagagt atgggttcca cctcgtcgct gtatgcagcc attgatctcg gttcgaatag
    20521 ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc cagacgctga cgcgaataaa
    20581 acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat gccctgtcta atgaagcaat
    20641 ggagcgcggt tggcaatgtc tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc
    20701 gcaaattcgc gttgtcgcta cggcgacatt acgtcttgcc gtcaatgcgg gtgattttat
    20761 tgccaatgcg caggaaatcc tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc
    20821 acgtctgatt tatcagggcg ttgctcacac aactggcggt gccgatcagc gcctggtggt
    20881 ggatataggc ggtgccagta ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt
    20941 gttcagcctg tcgatgggct gcgtcacctg gctggaacgc tattttgccg atcgtaatct
    21001 ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc
    21061 cgatgaatta cggtatcacg gctggaaagt gtgcgttggt gcttccggca ccgtgcaggc
    21121 gttacaggaa atcatgatgg cacaggggat ggatgaacgc attaccctgg aaaagttgca
    21181 gcaattgaaa cagcgagcca ttcattgcgg tcggctggag gaactggaga ttgacgggct
    21241 gacgctggaa cgtgcgttag tgttcccgag tggtctggcg atcctgatcg ccatttttac
    21301 cgaactgaat attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta
    21361 cggcatgttg catctggccg tcgagcagga tattcgcagc cgtacgctgc gtaatattca
    21421 gcgccgcttt atgatcgata ttgatcaggc gcagcgcgta gccaaagttg cggctaactt
    21481 cttcgatcag gtggaaaagg aatggcatct tgaagcaata agccgcgatt tgctcatcag
    21541 cgcctgtcag cttcatgaaa tcggcctgag cgttgacttt aaacaagcgc cgcaacacgc
    21601 tgcttatctg gtgcgtaatc tggatcttcc cggttttacc cccgcacaga aaaaattgct
    21661 ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc tcatcgctgc atcagcaaaa
    21721 tgccgtaccg ccgcgcgtcg cagaacaact ctgccgttta ctccgcctgg ccatcatttt
    21781 tgccagccgt cgccgtgacg atctcgtgcc agagatgaca ttacaggcta accatgaact
    21841 gttgaccttg acgcttccgc aaggttggct aacccaacat ccgctgggta aagagattat
    21901 tgctcaggaa agccagtggc agagctatgt ccactggccg ctggaagtgc attaatgagt
    21961 aagtgccgga tgcgatgctg acgcatcttt tccggccttg attatttccc tcgttttgcc
    22021 gccatcatcg ctttcagatt cgccagatgg ctttgccctt tctgcatccg ttcttcggcg
    22081 ctgaccactt tgcgctcctg ttcccaaatc agatcatcct gcggcagctc cagcaaaaag
    22141 cggctcggct ccgggcgcac cagctcgccg tactgacggc gttctttaca cagcgtaaag
    22201 gtcaattcct tctgggcgcg ggtaatgccg acataggcca gccgccgctc ctcatcgata
    22261 ttatcttcat cgatgctgct ctggtgcggc aaaaaccctt cttccatacc gaccatgtag
    22321 acataaggaa actccagccc ttttgacgcg tgcagagtca tcagttgcac ctgatccagc
    22381 tcttcttcac tctcaccacg ctccatcatg tcgcgcaaag taaagcgcgt caccacctgg
    22441 gtgagcgtca tcggctcatc cagttcgctg ccttccagca tctccgtcat ccagctaaac
    22501 agttggttga cgttcttcat gcgcatttcg gcggctttcg ggctgggcga tgtttcgtac
    22561 agccaggatt cataatccat gccgtggatc agatcgcgca ccgcggcaat cggctcccgc
    22621 tccgccagac gctggatttc tgccaaccag tgagtaaagc gggtcaatgc ttcataacca
    22681 cgtccgctaa gcgtctgact caggcccata tcaaagctgg cggtaaacat gcttttattg
    22741 cgcgtcatcg cccactcacc cagctttttc agcgtcgccg ggccaatctc tctcttcggc
    22801 gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa
    22861 gccagcaagt ccttgatttc aggacgagag aaaaacgacg taccaccaga aattttgtac
    22921 gggatgcggt tttgcatcag gaatttttca aacactcgcg actgatggtt accgcgataa
    22981 agaatagcgt aatctttgta ctgcgtttta ttgacgaagt gatgcgcaat cagttcacca
    23041 gtgacgcgct cggcttcatg ttcttcgtta ttcgcgctta aaactttaag ctctgtgccg
    23101 taacccagtt cggagaacag acgcttctca aagacgtgcg ggttattggc gatcaggatg
    23161 ttcgccgctt tcaaaatacg tccggaagag cggtagttct gctcaagctt aatcaccttc
    23221 agcgccggaa aatcctgact cagcagcacc aggttttgtg gacgtgcgcc gcgccaggag
    23281 tagatcgact ggtcgtcatc gcccaccacg gtaaagcgcg cgcggctgcc caccagcagt
    23341 ttcaccagtt catactggct ggtgttggtg tcctgatact catccaccag cagatagcga
    23401 attttgttct gccagcgctc gcggacttct tcattgcgtt gcagcagcaa cgtcggcaat
    23461 aaaatcagat catcgaagtc gagaacattg cacgctttca ggtgtgcatc atacagccca
    23521 taacaatggg caaaaatacg gtcccgctcg ccaatcgcac tggctgccgc ctgggacggt
    23581 gttttgagat cgttcttcca gttagagatg gtcgaaatca actgttgcag gagaactttg
    23641 tcatcttcaa tcagcccttc ggtcaactct ttgagcaacg ccagctgatc ggtatcgtca
    23701 aacagcgaaa agttcgcctt catccccagc gccgcatact cacgcttgat gatatccagc
    23761 cccagcgtgt ggaaggttga gatcatcagc ccacgcgcct ctttgcgacc cagcgtctgt
    23821 ccgacacgct ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt
    23881 cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt tattggtgat cacacgagtt
    23941 ttaccggaac ccgcgcccgc cagcaccagg caggggccgg taacgaattc gacagcttgt
    24001 tgttggccgg ggtttagacg cataggtgta ttgctcaatc ttcgaacggg gggaggattg
    24061 tagcagaatc gcatttgaat gtgaccgcgt gtaagccgct aaccgattgg ctgcggatgt
    24121 ttaaatcaga gagggaagac cgaaaccgga gttcatcgtg aatccattag acttagaaaa
    24181 tatcgggtcg taacgttgtg ttattacttt ttcagagttt actaaggaga ggtgtactcg
    24241 tggcgattaa tttcagtcct aaggttggtg aaatactgga ttgcaacttt ggtaactatc
    24301 ctgttagcca gaatggacag ttcagcacca cgtattacga tggccgcatt cctcctgaaa
    24361 tgataaaaaa caggttagt
//
LOCUS       DAZOXR010000051        23941 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZOXR010000051 DAZOXR010000000
VERSION     DAZOXR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23941
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.051"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..218
                     /locus_tag="ACHUNN_001744"
     CDS             <1..218
                     /locus_tag="ACHUNN_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576140.1"
                     /translation="HIGKHMIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGV
                     IVGFGAPVIGLIAGRKFINSLIKAKGK"
     gene            222..1052
                     /locus_tag="ACHUNN_001745"
     CDS             222..1052
                     /locus_tag="ACHUNN_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0576141.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            1148..1972
                     /locus_tag="ACHUNN_001746"
     CDS             1148..1972
                     /locus_tag="ACHUNN_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576142.1"
                     /translation="MPNHITNEIRVIGGTNKQRLDFIRAITNKKGLIDFNTICRRPKS
                     IEMEENNHIRRMASAMAGETVYDHMFGELKTPEQVELAMRESGMTRKEIRKIKEQALM
                     RLENYRRYGYYSWFDWSRANWGTKWNAYNIEMPVKRIPTRIKYGHNFRHTHVRAYAKR
                     IYKKRLARHAATGGELVIRFETAWSMPKPIYQAMARKFPHLEFVIRYADEDFGNNCGF
                     ILLRNGKWEYDYIAPANNKQSSEEKIKWRKFAFELCCPGITPQEYGLNEEYEYAGY"
     gene            2020..2760
                     /locus_tag="ACHUNN_001747"
     CDS             2020..2760
                     /locus_tag="ACHUNN_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576143.1"
                     /translation="MKRKIEEPKTTLNKHGEKIQTHPAFGLVKTSRVSTTGIRLFDSE
                     LEHRDYIEIGVYEAELSLDNERTRPQRSCNRRPLVEIRLSQAQWAAMVSSFGVGEGVP
                     CTISYKTIGDSIRVHDIAEQKSIRQKFNDQIKAATNSEIDRLNQQVSELGELVKKGRA
                     GKRDLEELYRKLASAVANLPENLCFATELMQEQMDEIVSSGKAEIEAYVAGTVMRKGL
                     AALGTESCINDDPTVSITKMESGKELLP"
     gene            2781..3170
                     /locus_tag="ACHUNN_001748"
     CDS             2781..3170
                     /locus_tag="ACHUNN_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576144.1"
                     /translation="MLLIQPGFGLKIKKGHMFGERDSKRKILSIRLPFITIHWLNRES
                     TDYWYKCARAAFNDPDWFVENHHAVRQAKRKANTTYMKAYQDAWEEHRGRYQKNIEKL
                     ESENMDLRRKLGEARRDIEAYKRLVSE"
     gene            3232..3657
                     /locus_tag="ACHUNN_001749"
     CDS             3232..3657
                     /locus_tag="ACHUNN_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576145.1"
                     /translation="MAAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPNQED"
     gene            3713..3988
                     /locus_tag="ACHUNN_001750"
     CDS             3713..3988
                     /locus_tag="ACHUNN_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576146.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            4204..4539
                     /locus_tag="ACHUNN_001751"
     CDS             4204..4539
                     /locus_tag="ACHUNN_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576147.1"
                     /translation="MKTIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            4539..4751
                     /locus_tag="ACHUNN_001752"
     CDS             4539..4751
                     /locus_tag="ACHUNN_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576148.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(5331..6545)
                     /locus_tag="ACHUNN_001753"
     CDS             complement(5331..6545)
                     /locus_tag="ACHUNN_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFR0576149.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            6747..7391
                     /locus_tag="ACHUNN_001754"
     CDS             6747..7391
                     /locus_tag="ACHUNN_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576150.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            7646..8731
                     /locus_tag="ACHUNN_001755"
     CDS             7646..8731
                     /locus_tag="ACHUNN_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFR0576151.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            8961..10877
                     /locus_tag="ACHUNN_001756"
     CDS             8961..10877
                     /locus_tag="ACHUNN_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0576152.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEIVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            10867..11610
                     /locus_tag="ACHUNN_001757"
     CDS             10867..11610
                     /locus_tag="ACHUNN_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576153.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHENEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NSPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            11620..12189
                     /locus_tag="ACHUNN_001758"
     CDS             11620..12189
                     /locus_tag="ACHUNN_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576154.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHDLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHSKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            12263..14578
                     /gene="nrdA"
                     /locus_tag="ACHUNN_001759"
     CDS             12263..14578
                     /gene="nrdA"
                     /locus_tag="ACHUNN_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0576155.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGIRYSDTAGNKLVHETFEAIQYYLLDASCQLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEIEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            14684..15826
                     /gene="nrdB"
                     /locus_tag="ACHUNN_001760"
     CDS             14684..15826
                     /gene="nrdB"
                     /locus_tag="ACHUNN_001760"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0576156.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWAEYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            15904..16773
                     /locus_tag="ACHUNN_001761"
     CDS             15904..16773
                     /locus_tag="ACHUNN_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFR0576157.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            16941..18044
                     /locus_tag="ACHUNN_001762"
     CDS             16941..18044
                     /locus_tag="ACHUNN_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0576158.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQTDDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            18046..18459
                     /locus_tag="ACHUNN_001763"
     CDS             18046..18459
                     /locus_tag="ACHUNN_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576159.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            18456..18932
                     /locus_tag="ACHUNN_001764"
     CDS             18456..18932
                     /locus_tag="ACHUNN_001764"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0576160.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            18932..19576
                     /locus_tag="ACHUNN_001765"
     CDS             18932..19576
                     /locus_tag="ACHUNN_001765"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0576161.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            19638..20057
                     /locus_tag="ACHUNN_001766"
     CDS             19638..20057
                     /locus_tag="ACHUNN_001766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576162.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            20067..20624
                     /locus_tag="ACHUNN_001767"
     CDS             20067..20624
                     /locus_tag="ACHUNN_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR0576163.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            20785..21594
                     /locus_tag="ACHUNN_001768"
     CDS             20785..21594
                     /locus_tag="ACHUNN_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576164.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            21777..22370
                     /locus_tag="ACHUNN_001769"
     CDS             21777..22370
                     /locus_tag="ACHUNN_001769"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR0576165.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            22558..22788
                     /locus_tag="ACHUNN_001770"
     CDS             22558..22788
                     /locus_tag="ACHUNN_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576166.1"
                     /translation="MSYPTNVVALVDSDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            23370..>23941
                     /gene="rnhA"
                     /locus_tag="ACHUNN_001771"
     CDS             23370..>23941
                     /gene="rnhA"
                     /locus_tag="ACHUNN_001771"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0576167.1"
                     /translation="MTTKTPARPQAKTRKKDKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAADMPHKKDIG"
BASE COUNT         6814 a         5105 c         5729 g         6293 t
ORIGIN      
        1 accacatagg aaaacacatg atcaaaatct atctattggc agtagccaca ggcgtttcag
       61 tggctctcat ctacggttta ctggttccgt cgctgatttc taccaagagt gatttagccg
      121 tcatgtttgg agttatcgtt ggttttggtg ctcctgtaat cggtcttatt gctggtcgta
      181 agtttatcaa ctcattaatc aaagcaaagg ggaaataagt aatgaagaaa ggtttacttg
      241 cagttgctct ggcggctatt tgcacaatgg gtcttactgg ctgtgatcgc gtggagcctg
      301 gatacgttgg catcaaagta aacaaattag gtgaagacaa agggatcggt gaagtggttg
      361 gcgttggtcg ccaatggaca ggtcttaaca ccgaacttta cgtattcccg accttcaaac
      421 aaatgaagac ctacgacgaa ccgttcacat tccagatgag tgacggtact gctattggtc
      481 acaaaattgg cgttgcgtat ctggttaatc gtgacaaggt aacgacggtg ttccagacct
      541 atcgcaaagg cgtagacgat atcaccgaat cagatctgcg tcagaaaatt gccgactctc
      601 taaaccgttt ggccagccgt atgaccactg actcatttat cgacggtggt aaggcgcaat
      661 tgctggacaa cgcactgaaa gatattcaga aagagatgtc tccggttggt attgaggtac
      721 tgagcctgtc atgggttgga aagcctgatt acccaaaaac cgtcattgaa tctatcaacg
      781 ccaaagtaac ggctaaccag cgtactctgc aacgtcagca ggaagttgaa caacgtaaag
      841 ctgaggcgaa tatgctacgt gaacaggcta atggtgaagc tgatgctatc cgtgctcgtg
      901 cgcaagcaga agcagacgcc attcgtctgc gcggtgaagc tctgcgtcaa aacccgaacg
      961 ttatggaact ggaagccatc aataaatgga atggccagtt accgcagtac atgactcaag
     1021 gggctaacac tcctttcatt acagtgaaat aactctcatt aagaagatca ggcgtccagt
     1081 tggacgccgt tttattctcg ttattatcac acacaagaaa acaatttgtt aaaaggttaa
     1141 gttaattatg cccaaccaca ttacaaacga aatccgagtc ataggtggta caaataaaca
     1201 acgcttagat ttcatacgtg ccataactaa caaaaaaggt ctaattgact ttaataccat
     1261 ttgcagacgt ccgaaaagta tagaaatgga agaaaacaat catatcaggc gcatggcctc
     1321 tgcaatggcg ggcgaaacag tttacgacca catgttcggt gaattaaaga ctccagaaca
     1381 ggtcgaatta gctatgcgtg aatcaggaat gacacgcaag gaaattcgca aaatcaaaga
     1441 gcaggctctt atgcggcttg agaattatcg tcgctatggc tattactcat ggtttgactg
     1501 gtcgcgagct aattggggaa caaaatggaa tgcctacaac atcgaaatgc ccgtaaaacg
     1561 tatccctaca cgtattaagt acggccataa tttccgccat acacacgttc gagcatatgc
     1621 aaaacgaatc tataaaaaac gtcttgcaag acatgccgca actggtggtg agttggtgat
     1681 tcgctttgaa acagcctggt ctatgccaaa accaatttat caagcgatgg ctagaaagtt
     1741 cccgcacctt gagtttgtaa tacgttatgc agacgaagac ttcggcaata actgcggatt
     1801 cattttactt cgcaacggca aatgggaata tgactacatt gcaccggcga ataacaaaca
     1861 atcgtcagaa gaaaaaatca aatggcgcaa gttcgccttt gagttatgtt gtcctggtat
     1921 cactccacaa gaatacggtc taaatgaaga atacgaatac gccggatatt aaaaaaaatt
     1981 gtcatttagc tttacgcatg aagcatagag gttaataaga tgaaaagaaa aattgaagaa
     2041 ccaaaaacga cccttaataa acatggggaa aaaattcaaa cacatccagc gttcggtctt
     2101 gtgaaaacaa gccgtgttag cacaactggc attcgcctat ttgattcaga actggaacat
     2161 agagactata ttgaaatagg cgtttacgag gcggaactgt ccttagacaa tgaacgaacc
     2221 cgcccacaaa gaagttgcaa tagacggcct ttagttgaaa ttagacttag ccaggcacaa
     2281 tgggctgcta tggttagcag ttttggagtg ggcgaaggtg tgccatgcac tatttcatat
     2341 aaaaccattg gagactcaat tagagttcat gatattgccg agcaaaaaag tattcggcaa
     2401 aaattcaacg atcaaataaa ggccgctacg aatagtgaga ttgatcgact taatcagcaa
     2461 gtttcagagc taggtgaact tgtgaaaaaa ggaagagctg gaaaacgtga ccttgaagaa
     2521 ctatatagaa aactagcatc tgctgtagct aaccttcctg aaaatctgtg ctttgctacc
     2581 gaattaatgc aggaacaaat ggatgaaatt gtctcatctg gaaaggcgga gattgaggcg
     2641 tatgttgcgg gcacggttat gcgtaaaggg ttagcagcat taggcacaga atcatgcatt
     2701 aacgatgatc ctactgtctc tatcaccaaa atggaaagcg gtaaagaatt actgccataa
     2761 atgcatctga ggtactgaca atgcttttaa ttcaacctgg gttcggttta aagataaaaa
     2821 aagggcatat gtttggtgag cgagattcca aacgaaaaat tctgtctatt cgattaccat
     2881 ttatcaccat tcattggtta aacagagagt caaccgatta ttggtataaa tgcgcaagag
     2941 cagcatttaa cgaccctgac tggtttgtag aaaatcacca cgcggttcgt caggcaaaaa
     3001 gaaaggctaa tacgacatat atgaaggcat atcaggatgc ctgggaagaa catcgtggcc
     3061 gataccaaaa aaacattgaa aaacttgaat cagaaaatat ggacctaaga agaaaactcg
     3121 gtgaagcacg tcgagacatc gaagcatata aacgtcttgt atcggagtga atatatcttt
     3181 gctttatttt ttcaaaggtg attattttcc tatttataag gagaagtaaa catggcagca
     3241 attaagaaac tctacgatgc cgcaaacgtg gctctggatg ttattgatga tgaagtagca
     3301 aaaggctttc ctgaacctga ttgggcgcat cagctacgaa acgctatcgc agaaatgacc
     3361 ccaccagatc caacccccga cgagacagac tggcagcgat tcatccgtat gtacgctcag
     3421 gaaataggtc caacgccaac ggcagagcag gcaatgctgc tgaaatactt caaagaggcg
     3481 ggagaggatt taccaattga tgactcagca tattggttcc actgcgcatg gcgtaagtat
     3541 gacgtgatat tcacacaagg catgggaagc aaagatatgg ttgtgtggca tctactccat
     3601 atagacacag ccgttgacag agttattgaa cagtttttcc ctaaccaaga agattgatcg
     3661 cctattcata actaacaaaa taagtaaaca ctaaccacaa aaggaaaaac acatgagagt
     3721 tttagttcga atcgttacca gcactgtcta tgacgtgttt ccgcttttta tggtcaaagt
     3781 cgatggcctt aacgatgaag aaactgacgc gctgatccag cgtactcttg ttgaatatac
     3841 aggtcatgac gctgattcag tgatggttga tgatgatggt gtttgttggc ataacggcaa
     3901 ctgttggtac gtagaagaga ctcaacaaat cagtaatgaa gatgctgaac atcttgagcg
     3961 tattttaagc atcagcactt ttgagtgagt ttacagtaaa gtttatataa gttagtatct
     4021 acctatcatg aagattttta ttgaatactt gttactcatc gtttcaatag cttttgtcat
     4081 cgactgcatt ttcactggtg tcattcgtaa agtcttttcc ccggtgcacg acgtagtcat
     4141 aaacgctttg gctatcgtgc tcgtatttaa ttcagcattt gatgtaatca aagaggtggc
     4201 agcatgaaga ccatcccatt cgcgctgttg tttctttctt cgatcgttgt ggccgacacc
     4261 actgtttatc agtgtgaaat gtctgtagcc gacgttaaga atggcgctct taccgacgtc
     4321 ataaaagcac catatggagc gatggtcgta gacagcggcg accagttcta tgttgtgcgt
     4381 gacgatcgag tgttgtcatc cccatatctc acaaatcgta atggcaaatt aaccggcgtc
     4441 ggagaagacc acttcgtata caacaaatac aagggcttct atggcgttca cgcttctcag
     4501 caaagctacc ttttcgatga ctgcaaggag gttgggtaat ggcattaaca ctggcaggtc
     4561 tggaaatcga gaaaacaagc ggctactggc gtgctaaggg tttcaagcag cctggcattc
     4621 ttgagcgtct ggaacgtgaa gatgggtata tcgtccacca gcggcgtgaa tggcgtatgt
     4681 acgatccaga aacaggaaaa ctgactacaa aagccggtac actttggggt ctgttaaaga
     4741 aaatacacta aatgcaaact gactgcggca cgttccgcag tcatatttca tagtcgtcac
     4801 cgctgacagc atacacaatc aactaccgct gatagcatat cgagagtcta tctcaccgct
     4861 cacagcatac tttactcgat tttttaccgc tgacagcata cttaagacat tgcatgaata
     4921 atgtgtaccg gtatgggtat aaccagaaca aaattaccgc tggcagcata cgaatgtctg
     4981 acatatgcca ttaattaccg ctgatagcat atccaaacaa aaattcctca ataaaacacc
     5041 gctgacagca tacgttctat cagggagtag caggcaataa atgcctttca ctacaagcaa
     5101 tcagcgcaat agcaatagaa tgttagtgag cgcaaaccta tatggaatgc actcttcgag
     5161 gttagtaacc actggggagg tatgacagag cattgagtgg tgatagatga tttaccgctc
     5221 acagcatacg ttcatctcac tataccgctg gtagcatatc tttaaccgtt cacagcatac
     5281 ttttcagaaa aatagccgct gatagcatac atttcaccgc tgacagcata tcaaagcagt
     5341 ttgagactat tggaaaggat ctcaatcatc ttgatatttt caggcgtcaa attctgcgaa
     5401 agttcggtta tcttgttgat aatgttctgt ttggcatcaa tttccccagc tttctcatct
     5461 ggttttttgg gttcgatgtc ttcaggtttt ggcggtgcga ctttgagttt tggattgcgg
     5521 ctgtgaatct ggatatagat cgaccgccca cgcttaatct cgctgtattc gagatagccc
     5581 aaatcttgga gagcttttaa gccgttacgt atagtctgat tctgcgagct gacattcctg
     5641 ctactcaaat tgagtcgcgc acgcaatcga gcaagcgata ccggcgcagg cttggttgga
     5701 agactttcga tgaaggtgta cagagcctgt gctgtttctt tgcgtggtag cttattgata
     5761 acctttaact gcaaaagaac cttatggtca aagcgatata gttcggccag cttcggttct
     5821 gcatagaaca ccaccgtatc tttctgctcg ttgtagtcca cgctattgat gaggtgcacc
     5881 atcagaagcg agatcttgtt agagccgtcg acgttctttt cttcatacgt tctctggaaa
     5941 gacagagttg tacgcatgat cttcaaaaga ctgtttgtaa gccggtcgcg gagtgttttg
     6001 cggatctgtg acgatggata gccacaaaac ttagcaaatt tcgtgatgct taactcgaca
     6061 cgaccgttag gttcgccgta ttctgccagc gaacgcacaa cgcccaccca cgttttgaaa
     6121 tcatgatcca tgtcgagacg aggaccggtt atcttgatat cggaatagcc ttcagaacgg
     6181 gctacttcga gctggacaag ctcctttgaa gcatcgatct catttggctt gttacgttta
     6241 ctgtattttg ttcccttaag cgtggggacg aacaacccca gtcgcatcaa cgcaattggt
     6301 tggactgtat tgttgctatt agggacaagt tcccctgtgt acaattcaag ggaaccttct
     6361 tcaaagttgt cgatgttatc ttctacttct ttgttatttt tacctttttt attttttgtg
     6421 gacatgtgga cacctttgtc attcaaccgc tgacagcata cttgatttgc cgctgacaga
     6481 ataccaaaaa cagttggcag catacggtga accgctgaca gactatcaat taccgctgac
     6541 agcatacatg aacatgactt cagaccagtc gtggcgcggc ttacagcgat cggggatctt
     6601 atttgatcta tataaggatc tatctatgga tctctttatt aggatctatc ctgtggatat
     6661 gtgaataatt aaaacaggca tttactacct tcggcgcacc tggtgggtta tcgttgcctc
     6721 ggctaacaat cacagaaaaa tgacatatgg atctaaaacg cacgcgctgg gttcgtcgtc
     6781 ttgaagacgg ctcctacact atcgaatcaa attccaacct gaataagcag aagttgcttt
     6841 gtgacatctg cggtatagcg gcgaagtgcc cgatctacga aaccagaatt aaacttgata
     6901 aggctggtgt gaattttcat ttaaacagtt gcatcaggta cgttccatta ctcgcatttc
     6961 gtaaaccgat catcggattg gatgccccct acttcaacac actccgttca ggtgtgacgt
     7021 ggcgagatcg tttatcacca gacaagctga tttgcctcgt atccgcagac acaggaaaaa
     7081 tcatccgttt tgggaaagta gacaaggttt actcaggccc agtagacgaa atgttgcgga
     7141 aacacagccg gtttaatcat ctctgtatgg gtggtgagaa aatcgagaag gtagaagaag
     7201 tgatccgcaa atcctacgga cactttctga ccaaagatag cctgctcacc gcaatctaca
     7261 tcagacatgt aaaacgtgag ttcgacctcg aataccacag cgaagaagag cttaaccttg
     7321 ttgacccacg tccaaaagct ggcgtcataa gcataaacgc agcgcgtaaa aagcccactg
     7381 acgcgctgta accctccaga tcgtatattg gcgtagatag aatctacgcc tcctcaaaat
     7441 agctctcata gcgttctaca gtgatcctgt cttattttta gtcatacaga caagcaaagt
     7501 tgcgccacaa caaataggta tatacttact tataaatttt gtatattaag acgctcgttt
     7561 cattcctaac ataccgttat gcatagttgt ttaccttctc attgctctta aaatttgtat
     7621 caaaataacc acaaaggaaa aacacatgac tttgccatac ggcgtcattt ctgactgcca
     7681 ctaccacaaa tgggatgcgt tctccacgac gaacgcagag gggcttaact ccagacttga
     7741 aatacagttg gaagcaacga aagaagcagc catcgccatg aagaaggccg gttgtaagta
     7801 catgttggtt gccggtgata catttcacgt ccgaggaact gtgtcccctt ctgttttgca
     7861 ttacgtaact gaaacgtaca agtggattat caacgagctt gatctgacag tagtaatgct
     7921 ggccggtaat cacgatcttg aaaccaacga ttcagtatat agcgccaacg cagcagcatc
     7981 gctgagttct atcggcgtgg taatcgtatg tggcaaacgc ccacactcaa taaaaattgg
     8041 tgatgtgact gtccacctga ttagctggcg taacaatcat gcggagctta tcagcgatct
     8101 gaaagcatta cgtaagagcg tagaaggtga taatcatgac gttgttatcc atacatccat
     8161 taacaaagcc attccaacaa tgcctgacgt cggtatcgat gcgcaggagt taaaggatat
     8221 cggctttcgt ctcgtgctta gtgggcatta ccacaaccac aaagaggtca ttcctggagt
     8281 tatcagtgtc ggtgcactga cccatcaaaa ttggggagat gttggatctc tggctggtta
     8341 catgatcgta aacccggacg gcagtttcag tcactacgaa accagtgcgc ctaaattcat
     8401 taacctggaa gatgatgttg ctgatgacca aattcgcggc aactacgtgc gtttccgcgc
     8461 cgtaattgag aacgatgaag aaggcattaa gtaccagaac atcctcaaaa caatgggcgc
     8521 aaaaggtgtc gtgtgcaact tcatccgtaa gtcatcaatg atggaaggga cagccagcac
     8581 aactgaaacc agcaaaatcg atagcctggg agagtcggta tctgcttatt gcaagattgt
     8641 ccacgatact gacggcggat ttgatctgag caagttggat attttgtgtc aggaaatcct
     8701 caccgaagcg gagagttcgg aggctgtgtg agacaaagtc gttatgggag ctttcgagac
     8761 tttgccatca cgatgaaaag acttgaacga ggccagacgg tgatgtttca caagccctac
     8821 ccgccacaag gaaatcccgt agcgttttat cttggaaggt taaccagaaa aggcgtattg
     8881 aggcgcagat ccttcccggc gcatacggag ttcagattga aagaaggtca aaagctaaca
     8941 cacggtatca aaggtgttat atgaagtttt taaagctcca ggttgagaat tttatggcta
     9001 tcgccagcgc ggaggtcgag ttagatcagc gtggtttagt gctcattcag ggtgttaata
     9061 gtgatgatag ttccgcatca agtaatggct ctggaaagtc aacgctaatg aatagcctga
     9121 tgtggtgtct ttatggcgaa acagctcatg gtgtgaaggg tgacgatgtg ttgtctaccg
     9181 accatgaaaa gaactgtcgt gttgcagtaa ccatcgagga tgaaggcaag agatatgcga
     9241 tcattcgtca ccgtaaacac aaagagttca aaaatcggct tatcgttcgt ggtgaagacg
     9301 gcgatatgac gaaaggcaaa gatgcgctga cgcaagagtt cgtcgagcgt ctgatcggtg
     9361 catctaaaga ggttttcatg gcttccatct atgcgagcca agaagctatg ccagatttac
     9421 ctggaatgtc cgacaaaaac ctcaaaacca tcgtagaaga agccgctggc gttgacagac
     9481 tgacacgcgc ctacgctatt gctcgtgagc gagctaatgc agctgccgca cgtatggatg
     9541 tggttaaaac caaattggag tcgacaatct cgaccattga ggcaacacag tcagaaattg
     9601 agtccgcgaa agcctcctct gaatcatggg aacaagagcg ttctaaacgt tatgacgatg
     9661 ccctggctgg gctggcaagt gccgaagttg agttaacgga agttgaactt gagatccgca
     9721 ctcttcccga acagatccgt gataccgaga aggcaatcga aagtgagcgc aaaaagttag
     9781 cctcaaaaga agaacatgac gccaagttgc tcaaagttcg tggtgcgata actgatattc
     9841 gggcaagcat caaagctaca gaaaatagtc aggctgatgc aatgaaccgc gcgcgtaatt
     9901 ttaagaccaa agcagaagag gttggtacta aagtgggatc accatgccct acttgtggca
     9961 aagcctactg cgaagaagat ctatcaacgg tgaaggagaa tttcattgaa caagcacgtc
    10021 aggaaattgg tcaggcgaag acacttgcag aggcaatggc taaacacaaa acgaatcttg
    10081 agaaagcgtt aagcattgag tctgcccttg ttaaaacgac acctgatgta acggctatca
    10141 ttgcccggat tgaagagctt acgaaacaac tctcatcttt gcgtcatcgt gagaaggaga
    10201 ttgttgctat tgagtctctt gtgactcgtg ctcgtactga ggtcgatcgt atatcaaaag
    10261 agattaatcc gtttattgct cttatcgcca gacacgaaga taacctggta tccagtaagt
    10321 ctaccttcaa gtccttaaaa gatgagttga aggctattca ggaacaaacg ttgctattgg
    10381 aaaaagctcg tcaggtctac tctcctgccg gggtgcgttc tcatattttg acgtctgtta
    10441 cgcctttcct gaatacacgc actgccgagt atctcaatac gttgtctgac gggaatatta
    10501 ctgctgagtg gtcgacgatg gatgtcacca aaaaaggtga gtatcgcgac aaattcaaca
    10561 ttagtgtgca gaagaaaggt tcaagtaagt cgttccagac cctctctggt ggtgagaagc
    10621 ggaaggttcg cattgcgtgt tctttggcat tgcaggatct ggttagtaac cgggcgagta
    10681 aaaacatcga tttgtttatc ggcgacgaaa ttgacgatgc actcgataca gccggtcttg
    10741 aacgcctcat gggtattctg gagtccaaag ctcgcgagcg aggtactgtg ctgattatct
    10801 cccataaaga gatgaagtca tggttccggg aaactattac gctggaagtt aaagaggggc
    10861 gcagctatgt cgtttaaatt aagccgctcg cagtttttgc aggtatttgc agtgatgcag
    10921 tcgataaaac tgatcaatgg gcatacttcc aatggtgcgg ctccacgtat tctgtggggc
    10981 agcaacaata ttgacggagt acaatttgcc gcgttgcttg gtctaatatc cgagacgcca
    11041 ttgatgcaaa gtttgaaatc actaccacct ggatgtattg cgccgatcct gattaatcct
    11101 tttgttgagg ggggatatct tcccaacgtc gggcctgggt ttattgcatc ccatgaaaat
    11161 gaagatctta acattaatag cgaagggttc tttgggggaa tgggtgcgca tcactgtatg
    11221 gctttcacca accttattcg acttgccaat aagcgggtgg atagtttggc atcgccaggt
    11281 gatgctttta ctggtttcct tatccaaagg agggataaaa agtacagtgc ggacaaacta
    11341 cagtttgttg gtaagtatgg agaaatggta gaaatcgaac ttcagctccc gcatgtttta
    11401 gcaaacgata gtgcagacag tcggaggctg ttgggcatca tgcgtcattt catagcaagt
    11461 ggcgttaaac atgccgtaga taaacgtgtc acgcaggaaa atgagtattc agactttgca
    11521 aactctcccc aaccaacgtt gcaaacggca atagtaacca attcgttgga ggcgagatta
    11581 ttggaaaacc ctatatgggg aacatggtaa ggagactata tgagtaaaaa aatcagcgta
    11641 gttggtgttg atccctcaat gagcaacttt gggcttgctg tgggcacttt agaccttgaa
    11701 acggacgaac ttgagattca cgaccttact cttgttgaga ctaaagcggg gagtaacaaa
    11761 aagaccgttc gtgtgaacag tgacgatctg cgccgagcca gtgaaatatg gcgtgttgcg
    11821 aagccaatca ttgataaggc aaatatggtt ttttgtgagc taccggttgg gagccaaaac
    11881 tctcgttcgc agacgtctta cggtatttgt atcggtgtac ttgcgtgtgt ggataagcca
    11941 ttgatccagg ttactccaaa cgaaatcaag cattttgtcg gcaataaact tactacatcg
    12001 aaagaagaga ttatccagtg ggctacgaaa aaacactcta aagcaccgtg gctgcgtcgt
    12061 aagcaatctg gacaggatgt tctcgtgaac aaaaacgaac atttggctga tgcggtggct
    12121 gccatccata ccggtatgca aacagatcag ttccgccagg tgcgcgatgt tcttaagtct
    12181 ctcatttgat ttcattgata ggtaagtgct tatctattaa catgggccac tatatttagt
    12241 ggccctcttt atttggtgat acatgataag catcgtaaaa cgtaacggcc aaacagagcc
    12301 gttatccgaa gaaaaataca accgcgtcgt aatgtatggc gtagaaggca ttcgtggtgt
    12361 aagcgcatcc gctgtagcaa tgggagctgc ggccagcatt tttgatggga tgaccaccag
    12421 ccagttgcat gaggctttgg ttaaatctgc cgctgatttg atctcaccag aagcaccaaa
    12481 ttactcacag gtggctgccc gcctgaacat ttttaaaatc cgcaaagatg ccttcggtcg
    12541 ttacgactat ccgaacttct accaacacat tgtcaagaac gttaacaagg gcgtttatga
    12601 caaggatttg ctgacacatt attcgtttga agagatcgaa gaactcggta attacattaa
    12661 gccgaaacgt gacgatcttt ttggctatgc agctacggtg cagttgcaaa gcaaatacct
    12721 cgttcaaaac cgtgttactg gtgagattca tgaggggccg caacatatct atatgctggt
    12781 aggcatgtgt ctgttccaga attgggaaga cgactgcgct ggcaaaacac gtatggagat
    12841 ggtcaaaggt ttctatgacg ttacaagtac gttcaaactg tctctgccca caccaatcat
    12901 ggccggcgtc cgtactccaa cccgtcagtt ctccagttgt gtgctgattg agtctggcga
    12961 tagtctgaaa gggattagtg cagcttcagc cgcaattatc gactacgttt cacgtcgtgc
    13021 tggaattggt attggttttg gccgtatccg tgcgctgggc agcgagatcc gcaatggtga
    13081 agccacccat accggagtta ttccattcct gaagcatttc cagacggctg ttaaatcttg
    13141 ttcgcaaggt ggtgttcgtg gtggcgcagc aacagcgttt tacccgatct ggcatcttga
    13201 agttgaaagt ctgctggtgg tgaaaaataa ccgtggtatt gatgaaaacc gcgttcgcca
    13261 tcttgattac ggcgtcatga gtaaccgtct aatgtaccgt cgactcgtca gaagcgagaa
    13321 catcactctg ttcagcccgc atgatgtgcc tgatatgtac gaagccttct tcacagacca
    13381 ggagctgttt gaaaagctgt accataaata cgaagccgat gattcaattc gcaagaagtc
    13441 agtacctgcc attgagctgt tctcatctct gatgcaggaa cgagcgtcca cgggccgaat
    13501 ttatattgcg aacgtcgatc atattaatga gcatggcgct ttcattcctg ctcttgcacc
    13561 tgtccgccag tcaaacctgt gtatggagat caccctacct acccgtccac tggcatttac
    13621 cgacgacccg aacggtgaga tcgcgctatg cactttatcc gcttttaacc tcggagccat
    13681 ccgttcactg gagtctctta aagaggtggc gttctatgcc gttgctgcac tggattcgtt
    13741 actggattat caagactatc cgatggaggc agccgaagtg cctgccaaag ctcgtcgtag
    13801 cctggggatc ggtgtaacca actttgctta ttacctggca aagaatggca ttcgttattc
    13861 tgataccgct ggtaataaac tggtgcatga aacgttcgaa gctatccagt attaccttct
    13921 tgatgccagc tgccaacttg ctgaagcaaa aggtgagtgt gactggtttg agcagaccaa
    13981 gtacgcaatt ggtcagttgc cgatcgacca ttaccgttct tcattagacg aaagtggcga
    14041 aaccaacttt gagttaaaga tgccgtggga agaactgcgt gaacgtattg caaaatacgg
    14101 tcttcgcaac tccacactga cggcacaaat gccatgcgag acttccagcc agatcactaa
    14161 ttccacaaac ggcatcgaac cgcctcgtgg accggtgtcg gtgaaatctt ctaaggacgg
    14221 catcgttaag atggtcgtgc ctgagattga aaaactgaag gaacagtatg aatacctgtg
    14281 ggatatgccg gacaaccgcg gctatctgac aaaggtggcg atcatccaga agttctttga
    14341 ccaggctatt tcggccaata ccaactatga cccttctcgc tttgaaggcg ataaagtccc
    14401 aatgatgacg ctactgtcag atttgcttct cgcctacaag atgggagtta aaacgcttta
    14461 ctaccacaac accagagatg gggcaggaaa gcgtgatgac gacgaaccgc agaatccact
    14521 gacgcaagct gtagccgtcg agccagaaga tgagtgcgac ggagcctgca aaatctgaca
    14581 tatggtgggg tgtatcccca ccttctcttt gatttgtaag ccttgtttaa acaaatagga
    14641 taacaacttg tttaaacaca tcaaaaagca aaaggaaaaa cacatgtcat attcaacgtt
    14701 ccgtttgggt gctaatgatg caaccaaaga gcctatgttc ctcggacaat ctgtcaacgt
    14761 ggcacgttac gatcagcaaa aataccgtga ttttgaaaag ttgattgaac gtcaattgtc
    14821 tttcttctgg cggccggaag aagttgatat ttcgagcgat cgtatcgact tcaacacgaa
    14881 gctgcgggac cacgaacgtc acatttttct gagcaatctc cgttatcaaa cgttactcga
    14941 ttcagttcag ggacgtagcc caaatgcaac gctgctgccg cttatctcta ttcctgaact
    15001 ggaaacgtgg gttgaaacat ggtctttctc tgagactatc catagccgca gctacaccca
    15061 cattattcgt ggcatggtgg acgatccgag cattgttttt gacggtattg ttacggatga
    15121 agaaatcatc aaccgagcga tcagtatctc tgctgaatat gacaggcttt atgggatgac
    15181 ctgcgagcgc cagtcgttag gcgagaagga atttgagcgt ctgtacgtaa atgaatatgg
    15241 ctgggagcca tatcctttgc accgtcagct tttccgcacg ttggtgtcca ttaatgcgct
    15301 tgaggcgatc cgtttctatg taagttttgc atgtacgttt gcctttggcg aacggaagtt
    15361 gcttgagggt aacaccaaaa ttatgcgctt tattgcccgt gatgaagctc tgcattgcga
    15421 aggaactgaa cgcatgatcc gcttcatgcg taccggtcgc gaaggtttat tgtggaaaga
    15481 gattgctgct gatgaagaaa acgtcattta cgacaccatg aaatcagtcg ccgaacaaga
    15541 aatgaactgg gccgagtatc tcttcaaaga cggttcgatg attggtttaa acgcggatat
    15601 tttgaagacc tatgtaaaat accgcaccaa tctggctatg aatcgtcttg gcctgaaggc
    15661 tttatttcca gaggttacca ccgatccgct ggtctggatg aacaagtggt tgttaaccga
    15721 cacactgcaa attgcaccac aagaggcaga gcaaagcaca tatctggtag gtcagatcga
    15781 ttctaccgtg gacaaggctt ctctaagcca gtttgcagac ctgtgaaccg atacaaagca
    15841 ttatgtaacc tggcaacgct gggccacaat ggatcacaag aattaagaag gaacaaaact
    15901 agcatgaact ttaccaaact gactgaccac ctgaaacttg ccaccgatcg tctcattgga
    15961 tttaagccag aaccatatga gttgcatgaa ggtcatggtg tagctactga aagtatttac
    16021 aagatggtcg atcagtttca tgaactcttc cagcatccga gacgcgttat gccgacacca
    16081 gagctgcttc gtctccgtgc aagcctgatt catgaagaag ctgtagtgga aggtattcca
    16141 gctgcaatga atggggatat tgagcaactg ctggatgcaa tggccgactt tttatacgtt
    16201 ggtgttggta cgatggtcgc catcaaaggt ggtatttcta ccggcatgac ctattacacg
    16261 caggaacaga gcattgatcg ctttatgcag acaatttttg tgcccggtaa cactgttttc
    16321 gatgatatgg caatgccatt tcaggaagct cgtgaggcgt catgtatgct cgaagagctg
    16381 gcagataaac ttgagaacaa gactgttaag gattctgagc tgattcagga actgcgccgt
    16441 gtcatgaaca aaatctatgt ggcgtgcatg atgacctatc gactggctga tttcctcggt
    16501 atcaatgtag tcgagctggt tggcgaaatt catcggtcta acatgacaaa attatggcct
    16561 gctgatgtag aggaacgtcg ccaggctgtg gccaactgca aatacgactc ttcagacctg
    16621 ggatttcgcc atgctgatgg caccgataag atgatcggtt ttcgaatttc cgatggaaag
    16681 attctgaagt ctccaaccta tagtgatgtc gatttatcct cctttgttga gcaagctaaa
    16741 gcctcagcaa tgtacggaat gatcaaaaaa taattgtagg tgattatcta ctaatgtata
    16801 tttgatttgc gtataaaact ctggaacgac tattcttttt ggtggcctat ggccaccatt
    16861 ttttatctat ctggtcttgt tttatcaatg aatgtaaact cacgtgataa ataattgatt
    16921 acttatcttc gtgaggtttt atgtcactcc ttttgaatcg tgagcatacg aacggtcagg
    16981 taacaaacgc atcgtatgca aaagttattg agacggtgct taaaagcggc gtgcagactg
    17041 atgatcgcac aggcactggt actttaagca cctgctacgt tccctcttac tacatgctta
    17101 ctggtgggac tgtgccgctt atttctggaa aggcggtaaa tcttaagcca ctgcttgtcg
    17161 aacttgagtg gtatctgaaa ggcacgggca acatccaatt tcttaaggat aacggcgtta
    17221 agatttggga tgcatgggcc gatgagaatg gcgatttggg gccggtttac ggtaagcagt
    17281 ggcgtcgatg ggaagatacc cgcatcgtga gccatagtga atatctgagc aagatcgcta
    17341 ctttccgtga acgcgggtac aaagtcgagg gatacctggg tatcagtgaa gatcgcgtag
    17401 tgctgtcccg tgaaatcgat cagctacagc gtattgtcga cacactgcgc acgaacccta
    17461 ccgatcgtcg catcatgctt aacgcatgga acgtaggcga gcttgaggat atgaaactgc
    17521 caccttgcca ctttgtcttc tctttgtgga gtcgtgagct tgattttgaa acccgtttaa
    17581 cgatggcaac tgacattggt cttcaacaca gtcgcttcgg ttatgagtct atctacacca
    17641 agatgctata cgatctggag atggacggca gtgttactga aactgaactg gatgaacttg
    17701 gaatccccaa acgcatcctc aactcctgcc tcgtacagcg tagcgtagac acttttgttg
    17761 gtatgccatt caatattgct ggctatggca ttctcactca ttttctcgcg aagattacgg
    17821 gtcacatggc cggtgcattt gtgcattttg gctttgacgt gcatttgtac aacaaccaca
    17881 tggaaggtgt gtgtgagcta atgaaacgac aggctccaga gcattcagat ccggtcgtta
    17941 ttttccctca tgaatggtct gagctggatg atttcaaatg ggacgatatt ctgattcttg
    18001 gctatgaccc tctaccgtgg atcaaggttc cagtggcggt gtgatatggc aagaggtatg
    18061 tatgtcttat gcgaaattga aggtgtgctg gcaaatacca gccatcgtaa atctgtacct
    18121 gacacggatg caggccagct cattgccggt gatgaactca ttttccccac cagccgtatg
    18181 ttgcgtggtt ttgctcgctc aggggctgaa gtggtgctta tcagtagccg ctctgaaact
    18241 cttgaagcgc ccactaaacg atggctgaaa gattttggcg ttgattacga ctggcttcat
    18301 ctcgtaccga atggcaccag ttatgagaag catattaagc gcacattagc ggagcataaa
    18361 ggcgatctgc ttatcgctgc gctggtgcac gatcctcgac tccgtgccgc tttagctgac
    18421 tctcatcata gaccggtcat ctatgaggtg agcaaatgaa gatgatagct gctgttggcc
    18481 gtaactatga gatcggcata gcgaacgaac tgccctggcg ttgttctacc gatctgaagc
    18541 tatttaagag actcaccaaa aacgccactg tcgttatggg gcgtaaaacg atggaaagtc
    18601 tcaaacgccc tcttccagag cgtcataacc tcgttttgac gcgctctcat ggctttgtac
    18661 caaatggatt ctaccctgct ggtgtggatg atgtgttgcg attaccagag cctgtgtggg
    18721 tgattggcgg ggaacaaatt tactcgctat tcatgccgca tgttgaagag atttggctct
    18781 cccacatcgg cgttgatgtg ccaaacgccg atgcattctt cccggcaagc atgatgcgta
    18841 atttaggctt tgtgcctgtt gaaacagttt ttacccaacg agccagcgag gaagagcctg
    18901 gcttttcgca gatcgtatac agaaggtcgt aatggattac cggattggga tcactggtgc
    18961 tcagggcagt gggaaaacaa ccctggcaaa atatatcgac aaacattacg gaatccctta
    19021 cgtggatgct ggtgtcggaa gtttgatgag ccgactcggt gttcgagtag gtgagtctat
    19081 gcctctatat gagcggcttc agattcaaat ggaaatcgca aagcatatag agctacttac
    19141 gcgtggtgtt gaaggctttg ttatcgatcg cacacctgct gatgttatgg cctacacgtt
    19201 ggatttggtc ggccatacca atgaagatcg atgtattgag ttagccctcg atatcgaaaa
    19261 gttttgccac aaaactgcta tttcaaactt taacgccatt gctggcctac gcccgggagt
    19321 cgctctctca gagcgagatt acttgcgatc acaacgagca tcattagacc gtctgtatgt
    19381 cgctcgtatt gatgcgttga tgtgcgggga actgacaaaa attcacctgc atccgcaaag
    19441 gggggatctg caaaccttcg tcgtttccaa ccgatatcgc acggttgaag caagagccag
    19501 atcagtgatg agaatgctag ataacgctgt agaaaagata gaaaaccggt tctgtggtcg
    19561 agtgaccgtt cattagaaat tgttcgcctc ttcgacattg cgacaataaa actctcaaaa
    19621 tgggttaagg ataaaaaatg tttagtgaaa tgttgcttga agatgaactg gatcggaaaa
    19681 caacagaggc tttgattcgt gtagcggacg aacattcccg gtcgcttatg agcgatcgag
    19741 aggctcgtct ggctattcgt gccatattcg aaactgcgca ggggcttgtt ggcgcacaag
    19801 taggtgaagc cattaacatc gccatgtctc agttcagtga agacagtaaa aagcctctgt
    19861 ttcctatgca tttgatgctg gctggtggca cggtgcttta tatctctgtt tgtctggata
    19921 gcaaccaaat caatattctc aacactgcgt caggtaagtg gaaagatccg attgtctgtg
    19981 aaaccagtga agaaactttg aaaaaagcgg ctcaatttgt acgtagcgca ctacttaagg
    20041 gcgctaagaa gttgtaagga gttctgatga caacgattgt tgcaggcatc gatatcgagt
    20101 ctacgggact ggatttcctt gctggtcata aaattattga aatcgcaatt acccgctatg
    20161 aactggagac acagagacat attgatagtc tggagatgcg ttttaaccct cgcagaaaca
    20221 tagatccgaa agctcaagcc gttcatggca tttcattaga acagctcgca gctgaacctt
    20281 tgttgtcaaa tcatgctagc gaaattggcg cttatatgga ggcatgtagt gcgtgggttg
    20341 ctcataacgg cgaagcgttt gatataccat ttattagaca cgagttttca gggtatggag
    20401 taagactgcc agacgttccc gttatagata ctatgttatc gggattgtgg gccacagaag
    20461 acggtaaacg tcctcgcctt gaagaattgg ccttctctct tggctttata tacgatcatg
    20521 ccaaagcaca tagtgcctta tatgacacaa acttaatgat gcaatgcttc tttaaggcac
    20581 gtaataaata cggatttttt aaattaccct ctgaaattgt gtaaaacaaa agcctgcttt
    20641 aaagagagtt tgaagcaggc tttcttttaa agaacagtcg cctttcaatc atttcctgcc
    20701 tgtatttaat acttttccgc ctgataggtt tagtcaaaat gtagccatcg aaacgcaaat
    20761 gcaactaaac agaaggagac ttacatgagt tcggttgaaa atgtaatgac aaatgatgat
    20821 ctggatgagc tgacagccat gttgcaatca cttgatgaac cagtaaaaaa agctgcacag
    20881 gttgaaaata ctgatgatat tgacgatctg cttctcggcc tcgatgctgg cgtagccatg
    20941 agttctgatg atgttgccga agaactgttc aatgaagaaa aagcaggtga tttcagctct
    21001 gctttaaatg agttggagtt agcgcatgag cctataaacg taattaacgc tgaaagtggt
    21061 gaagctgccg aaaatgagcc agaacagttg gggatcattg aggttgaaga ttgtgttgag
    21121 gttaatgatg aattaaaagt tcaacagtca aatgatagca atacaaataa aaaaactcgt
    21181 gctgcaagag gtcctagttt tactttaagt gataaagatg attcgttttt caataaagca
    21241 ggcttagaaa aagatatttt cttagacgct tacgaaaacg cgcctgtcaa agcgaaggat
    21301 aagatattaa accttcttaa ttggtttagc ggaggtccag atattagtgt ttacacggta
    21361 atttccatga gacaccttct tacagaaaag aaggctacaa gtaatagtat taagattgct
    21421 ttaatgagca atccagaaaa accgtatccg cttaacactg cgtcaactca ggctgggcaa
    21481 atgatggctg tatttccagc tacaggaatt gccgttagag acggtggaaa tctaacattg
    21541 aacgaagaat caccgatcgt taagaagttt gtcgcggagt acactattgg atgacgttcc
    21601 cctactgaaa ataaagccca tagagagctt tatagtgctg ggtaagataa cccataccca
    21661 gcaccaaaaa aacgcgccag agagcttctt gttcgcattt ctggcgcgtt ttatttgatt
    21721 gccatacata aaatcaaatg caaaaatagg tatttactta cctatcgaga aagaagatga
    21781 ttgcagccga aaaaatcaaa cagcgaaagc gcgacaactc tcttcgtgac ctctggagaa
    21841 cacctgattg gctgttttct gccattcaac gttatcttgg agtgacattt gatgttgacg
    21901 ttgcctgcaa caaggacaac gcaaagctgc ctaatttcat aggcattgag cgtgatgctt
    21961 tgaaatctga atggggacag ccaggtacaa ttgccttcct caatccaccc tactccaaaa
    22021 tctccccctg gattgatgct gctatacgtg agcaggctcg cggagttact acagtgatgc
    22081 taattcctca atccctcgat acaaagtggt atgagcgtgc aacagagtat gcgaatgaga
    22141 cgattattct gtctggtggc cgcgtagcgt ttgtcgagcc tgacgtcaat ttgggtcagg
    22201 tagaagtaaa catcaacccc ggtggcagta tgctcgttgt ttttcgagga ttctgtcagg
    22261 acgctgggca ctctataagc aagatccctt tggacgtcat gaaaagtctg ggagggtatg
    22321 atcctgcgaa tgtgatcagg aaaaaaagac catcaaagaa ggctgcttag tttgttctgg
    22381 cgtctgtaat tagcctgctt ctgtatatat ataaataact acatattaat tattaatata
    22441 cggaagcagg ctgttttgta tcagagactc cagacctgaa catcactaca gaatccacta
    22501 gacccccttc ccagacgctt taaaatcgat tttatgaacc actttaagga aaccaacatg
    22561 tcatacccga ctaatgtcgt tgcgctcgta gacagcgatt ttctggccca ggctcgtgaa
    22621 atgatgaaag atcgtgagca ggctttcaac ttgtacgagt gggcaattaa gtgcttgcat
    22681 cttggggagc atcgcgaact tgttgaacag cttttaggtg agttgatcaa cgaggtgttt
    22741 gccttgaatg ttcaactaca tggtcgagaa aataatcaat cgaaatgata gataagtaca
    22801 aactattcat aagatgaatt gtaagtgcta agatctgata gtttccagtc gtagactgga
    22861 gactcgacct gatgggtggg ggtaagcgtc actggcgtca ggtttaaaaa agctcactac
    22921 cagcgtagaa ccggtgccgt ttaggtgtcg gggaaggggg aaccaaagtg agcggagaca
    22981 agggtcactt tatgattgtc gagtctgggg tgtttcgaga ggttgaatcc agtactcccc
    23041 ttcataaagt gtgggaagat ctcggttctg gggtgctgtc atccataact tcccaagtct
    23101 aagctggcag tagacttaga ccatagcttt tcaggttatg aaacgaccag gttggtgagg
    23161 aattttatac tcacctccct gggagagtat tacctgaaaa gacaacctct cacttcgttc
    23221 gaggtgaact tcactcactt cgttcgttca gttcaggttt ataaaaacct gttctgggaa
    23281 gtaatttgtt tattttaata attattaaca cgcacgcgtg tgcgcacgcg cgaggaaaaa
    23341 tcggcgcggc gcttgattca ggagtttata tgacaacgaa gacaccagcc cgaccgcaag
    23401 caaaaactcg caaaaaagac aaaaacaaaa attctccccg caccaatccc acaacgcctg
    23461 tcgtagagtt caatccccag cttaaaaccg tgaaaatctt tagtgatggc tcttgcctta
    23521 aaaatccggg tggcccgggc ggttacggta tcgttctcca gtatcgtggc gaggaacgcg
    23581 agttctcaga tggttttcat agcaccacca ataaccgcat ggagatgatg ggggcactta
    23641 tcgggctgga gcgtttgaaa tatccatgca acgttatttt gcactctgat agccagtatc
    23701 tgaaaaacgg catgacacag tggatgaaat ggtggaaacg caatggatgg atgacttctg
    23761 acaaaaaacc ggtaaagaat gttgatctgt ggaagcgtct ggatgaggcc gcaagtcgac
    23821 ataatgttcg ctggaagtgg gttaaaggtc acgccgggca tcgtgaaaat gaaatatgtg
    23881 atcgactcgc gaagatcgca gctttttcag cagcagatat gcctcacaag aaagatattg
    23941 g
//
LOCUS       DAZOXR010000052        23934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZOXR010000052 DAZOXR010000000
VERSION     DAZOXR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.052"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..164)
                     /locus_tag="ACHUNN_001772"
     CDS             complement(<1..164)
                     /locus_tag="ACHUNN_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0576168.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTV"
     gene            complement(222..455)
                     /locus_tag="ACHUNN_001773"
     CDS             complement(222..455)
                     /locus_tag="ACHUNN_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR0576169.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            844..1389
                     /locus_tag="ACHUNN_001774"
     CDS             844..1389
                     /locus_tag="ACHUNN_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0576170.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1364..3289
                     /locus_tag="ACHUNN_001775"
     CDS             1364..3289
                     /locus_tag="ACHUNN_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0576171.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3286..3492
                     /locus_tag="ACHUNN_001776"
     CDS             3286..3492
                     /locus_tag="ACHUNN_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR0576172.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3489..5090
                     /locus_tag="ACHUNN_001777"
     CDS             3489..5090
                     /locus_tag="ACHUNN_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0576173.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5071..6390
                     /locus_tag="ACHUNN_001778"
     CDS             5071..6390
                     /locus_tag="ACHUNN_001778"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0576174.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6400..6732
                     /locus_tag="ACHUNN_001779"
     CDS             6400..6732
                     /locus_tag="ACHUNN_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0576175.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6788..7813
                     /locus_tag="ACHUNN_001780"
     CDS             6788..7813
                     /locus_tag="ACHUNN_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0576176.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7855..8253
                     /locus_tag="ACHUNN_001781"
     CDS             7855..8253
                     /locus_tag="ACHUNN_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0576177.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8265..8618
                     /locus_tag="ACHUNN_001782"
     CDS             8265..8618
                     /locus_tag="ACHUNN_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0576178.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8630..9208
                     /locus_tag="ACHUNN_001783"
     CDS             8630..9208
                     /locus_tag="ACHUNN_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0576179.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9205..9600
                     /locus_tag="ACHUNN_001784"
     CDS             9205..9600
                     /locus_tag="ACHUNN_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0576180.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9608..10348
                     /locus_tag="ACHUNN_001785"
     CDS             9608..10348
                     /locus_tag="ACHUNN_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0576181.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10364..10786
                     /locus_tag="ACHUNN_001786"
     CDS             10364..10786
                     /locus_tag="ACHUNN_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0576182.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10768..11202
                     /locus_tag="ACHUNN_001787"
     CDS             10768..11202
                     /locus_tag="ACHUNN_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0576183.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11195..13756
                     /locus_tag="ACHUNN_001788"
     CDS             11195..13756
                     /locus_tag="ACHUNN_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0576184.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13753..14082
                     /locus_tag="ACHUNN_001789"
     CDS             13753..14082
                     /locus_tag="ACHUNN_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0576185.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14082..14780
                     /locus_tag="ACHUNN_001790"
     CDS             14082..14780
                     /locus_tag="ACHUNN_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0576186.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14786..15529
                     /locus_tag="ACHUNN_001791"
     CDS             14786..15529
                     /locus_tag="ACHUNN_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0576187.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15526..16098
                     /locus_tag="ACHUNN_001792"
     CDS             15526..16098
                     /locus_tag="ACHUNN_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0576188.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16159..19656
                     /locus_tag="ACHUNN_001793"
     CDS             16159..19656
                     /locus_tag="ACHUNN_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR0576189.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19727..20243
                     /locus_tag="ACHUNN_001794"
                     /pseudo
     CDS             19727..20243
                     /locus_tag="ACHUNN_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20308..23391
                     /locus_tag="ACHUNN_001795"
     CDS             20308..23391
                     /locus_tag="ACHUNN_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0576190.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23391..>23934
                     /locus_tag="ACHUNN_001796"
     CDS             23391..>23934
                     /locus_tag="ACHUNN_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0576191.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTST"
BASE COUNT         5643 a         6009 c         7324 g         4958 t
ORIGIN      
        1 aaaaccgtat tatcgttttc aagaacaaga tgaatattct ctgtggctaa atagtaaatg
       61 taatgtgaga cattgtgacg ttttagttca gaataaaacc agtgatagtt taaattattt
      121 cgcactttat cgaatatttg tttaaaaatg gcaacctgag ccattgtagt accttccatg
      181 tgatatgagg gggcgtagtc tgcacgatta tctaaattgc ttcaatctgg tctaacctgt
      241 tttctgagca attcagtaat gtcactcttt tctttgtttg cttcaggcga aactcttttt
      301 tctgagcaca gtctccggcg gcaggcttca atgacccagg ctgagaaatt cccggaccct
      361 ttttgaacaa gagcgatgtt aatttgttca atcatttggt taggaaagcg gatgttgcgg
      421 gttgttgttc tgcgggttct gttcttcgtt gacatgaggt tgccctgtat tcagtgtcgc
      481 tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata
      541 cctgcgtcat aattgattat ttgacgtggt ttgatggcgt agatgcacgt tgtgacatgt
      601 agatgataat tattatcatt ttgcgggtcc tttccggcga tccgacaggt tacggggcgg
      661 aaggcgcgcg ggttttcgct atttatgaca attttccggt ttaaggcgtt tccgttcttc
      721 ttcgccgtaa cctaatgttt ttatttaaaa caccccctga aaagaaagga aacgacaggt
      781 gctgaaaacg ggctttttgg cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga
      841 acaatggaag tcaacaaaaa gcagctggct gacattttcg gtgcgagtat ccgtaccatt
      901 cagaactggc aggaacaggg aatgcccgtt ctgcgaggcg gtggcaaggg taatgaggtg
      961 ctttatgact ctgccgccgt tataagatgg tatgccgaaa gggatgctga aattgagaac
     1021 gaaaagctgc gccgggaagt tgaagaactg cggcaggcca gcgagacaga tctccagcca
     1081 gggactattg agtacgaacg ccatcgactt acgcgtgcgc aggccgacgc acaggagctg
     1141 aaaaatgcca gagactccgc tgaagtggtg gaaaccgcat tctgtacttt cgtgctgtcg
     1201 cggatcgcag gtgaaattgc cagtattctc gacgggatcc ccctgtcggt gcagcggcgt
     1261 tttccggaac tggaaaaccg acatgttgat ttcctgaaac gggatatcat caaagccatg
     1321 aacaaagcag ccgcgctgga tgaactgata ccggggttgc tgagtgaata tatcgaacag
     1381 tcaggttaac aggctgcggc attttgtccg cgccgggctt cgctcactgt tcaggccgga
     1441 gccacagacc gccgttgaat gggcggatgc taattactat ctcccgaaag aatccgcata
     1501 ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc atcatgaatg cgatgggcag
     1561 cgactacatc cgtgaggtga atgtggtgaa gtctgcccgt gtcggttatt ccaaaatgct
     1621 gctgggtgtt tatgcctact ttatagagca taagcagcgc aacacactta tctggttgcc
     1681 gacggatggt gatgccgaga actttatgaa aacccacgtt gagccgacca tccgcgatat
     1741 tccgtcgctg ctggcgctgg ctccgtggta tggcaaaaag caccgggata acacgctcac
     1801 tatgaagcgt ttttccaatg gtcgtggctt ctggtgcctg ggcggtaaag cggcaaaaaa
     1861 ctaccgtgaa aagtcggtgg atgtggcggg ttatgatgaa cttgctgcct ttgatgagga
     1921 tattgaacag gaaggctctc cgacgttcct tggcgacaaa cgtattgaag gctcggtctg
     1981 gccaaagtcc atccgtggct ccacgcccaa agtgagaggc acctgccaga ttgagcgtgc
     2041 agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc tgcccgcact gcggggagga
     2101 gcagtatctt aaatttggcg acaaagagac gccgtttggc ctcaaatgga cgccggatga
     2161 cccctccagc gtgttttatc tctgcgagca taatgcctgc gtcatccgcc agcaggagct
     2221 ggactttact gatgcccgtt atatctgcga aaagaccggg atctggaccc gtgatggcat
     2281 tctctggttt tcgtcatccg gtgaagagat tgagccacct gacagtgtga cctttcacat
     2341 ctggacagcg tacagcccgt tcaccacctg ggtgcagatt gtcaaagact ggatgaaaac
     2401 gaaaggggat acgggaaaac gtaaaacctt cgtaaacacc acgctcggtg agacgtggga
     2461 ggcgaaaatt ggcgaacgtc cggatgctga agtgatggca gagcggaaag agcattattc
     2521 agcgcccgtt cctgaccgtg tggcttacct gaccgccggt atcgactccc agcgggatcg
     2581 ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa agctggctga ttgatcggca
     2641 gattattatg ggccgccacg acgatgaaca gacgctgctg cgtgtggatg aggccatcaa
     2701 taaaacctat acccgccgga atggtgcaga aatgtcgata tcccgtatct gctgggatat
     2761 tggcgggatt gacccgacca ttgtgtatga acgctcgaaa aagcatgggc tgttccgggt
     2821 gatccccatt aaaggggcat ccgtctacgg taagccggtg gccagcatgc ctcgtaagcg
     2881 aaacaaaaac ggggtttacc ttaccgaaat cggtacggat accgcgaaag agcagattta
     2941 taaccgcttc acactgacgc cggaagggga tgaaccgctt cccggtgccg ttcacttccc
     3001 gaataacccg gatatttttg atctgaccga agcgcagcag ctgactgctg aagagcaggt
     3061 cgaaaaatgg gtggatggca ggaaaaaaat actgtgggac agcaaaaagc gacgcaatga
     3121 ggcgctcgac tgcttcgttt atgcgctggc ggcgctgcgc atcagtattt cccgctggca
     3181 gctggatctc agtgcactgc tggcgagcct gcaggaagag gatggtgcag caaccaacaa
     3241 gaaaacactg gcagattacg cccgtgcctt atccggagag gatgaatgac gcgacaggaa
     3301 gaacttgccg ctgcccgtgc ggcactgcat gacctgatga caggtaaacg ggtggcaacg
     3361 gtacagaaag acggacggcg agtggagttt acggccactt ccgtgtctga cctgaaaaaa
     3421 tacattgcgg agctggaggt gcagaccggc atgacacagc gacgcagggg acctgcagga
     3481 ttttatgtat gaaaacgccc accattccca cccttctggg accggacggc atgacatcgc
     3541 tgcgtgaata tgccggttat cacggcggtg gcagcggatt tggtgggcag ttgcgggcgt
     3601 ggaacccacc gggtgaaagt gtggatgcag ccctgctgcc caactttacc cgtggcaatg
     3661 cccgcgcaga cgatctggta cgcaataacg gctatgctgc caacgccatc cagctgcatc
     3721 aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg
     3781 gcatcgggga ggaagacgcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt
     3841 ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga
     3901 ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg
     3961 ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca
     4021 gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca
     4081 gcggtgcggc gctgggatat tacgtcagcg aggacggcta tcctggctgg atgccgcaga
     4141 aatggacatg gataccccgt gagttacccg gcgggcgcgc ctcgttcatt cacgtttttg
     4201 aacccgtgga ggacgggcag acccgcggtg caaatgtgtt ttacagcgta atggagcaga
     4261 tgaagatgct cgacacgctg cagaacacgc agctgcagag cgccattgtg aaggcgatgt
     4321 atgccgccac cattgaaagt gagctggata cgcagtcagc gatggatttt attctgggcg
     4381 cgaacagtaa ggagcagcgg gaaaggctga cgggctggat tggtgaaatt gccgcgtatt
     4441 acgccgcagc accggtccgt ctgggaggcg caaaagtgcc gcacctgatg ccgggggact
     4501 cactgaacct gcagacggct caggacacgg ataacggcta ctccgtgttt gagcagtcac
     4561 tgttgcggta tatcgctgcc gggctgggtg tctcgtatga gcaactttcc cggaattacg
     4621 cccagatgag ctactccacg gcacgggcca gtgcgaacga gtcgtgggcg tactttatgg
     4681 ggcggcgaaa attcgtcgca tcccgtcagg cgagccagat gtttctgtgc tggctggaag
     4741 aggccatcgt tcgccgcgtg gtgacgttac cttcaaaagc gcgcttcagc tttcaggaag
     4801 cccgcagtgc ctgggggaac tgcgactgga taggctccgg tcgtatggcc atcgatggtc
     4861 tgaaagaagt tcaggaagcg gtgatgctga tagaagccgg actgagcacc tacgagaaag
     4921 agtgcgcgaa acgcggtgac gactatcagg aaatttttgc ccagcaggtc cgtgaaacga
     4981 tggagcgccg cgcagctggt cttaaaccgc ccgcctgggc ggctgcggca tttgaatccg
     5041 ggctgcgaca atcaacagag gaggagaaga gtgacagcag agctgcgtaa tctcccgcat
     5101 attgccagca tggcttttaa tgagccgctg atgcttgaac ccgcctatgc gcgggttttc
     5161 ttttgtgcgc ttgcaggcca gcttgggatc agctgcctga cggatgcagt atccggcgac
     5221 agcctgactg ccggagaggc acccgcggcg ctggcgttat ccggtgatga tgacggacca
     5281 cgacaggccc gcagttatca ggtaatgaac ggcatcgccg tgctgccggt gtccggcacg
     5341 ctggtcagcc ggacgcgggc gctgcagccg tactcgggga tgaccggtta caacggcatt
     5401 atcgcccgtc tgcaacaggc tgccagcgac ccgatggtgg acggcattct gctcgatatg
     5461 gacacaccgg gcggaatggt ggcgggagca tttgactgcg ctgacatcat cgcccgtgtg
     5521 cgtgacataa agccggtatg ggcgctggcc aatgacatga actgcagcgc aggtcagctg
     5581 cttgccagtg ccgcctcccg gcgtctggtc acgcagaccg cccggacagg ctccatcggc
     5641 gtcatgatgg ctcacagtaa ttacggcgct gcactggaga aacagggcgt ggaaatcacg
     5701 ctgatttaca gcggcagcca taaggtggat ggcaacccct acagccatct accggatgat
     5761 gtccgggaaa cattgcagtc ccggatggat gcaacccgcc ggatgtttgc acagaaggtg
     5821 tcggcatata ccggcctgtc cgtgcaggct gtgctggata ccgaggctgc agtgtacagc
     5881 ggtcaggagg ccattgatgc cggactggct gatgaacttg ttaacagtac cgatgcgatc
     5941 accgtcatgc gtgatgcact ggatgcacgt aaatcccgtc tctcaggagg gcgaatgacc
     6001 aaagagactc aatcaacaac tgtttcagcc actgcttcgc aggctgacgt tactggcgtg
     6061 gtgcaagcga cggagggcga gaacgccagc gcggcgcagc cggacgtgaa cgcgcagatc
     6121 accgcagcgg ttgcggcaga aaacagtcgc attatgggga tcctcaactg tgaggaggct
     6181 cacggacgcg aagaacaggc acgcgtgctg gccgaaaccc ccggtatgac cgtggaaacg
     6241 gcccgccgta ttctggccgc agcaccacag agtgcacaga cgcgcagtga cactgcgctg
     6301 gatcgtctga tgcagggggc accggcaccg ctggctgcag gtaacccggc atctgatgcc
     6361 gttaacgatt tgctgaacac accagtgtaa gggatgttta tgacgagcaa agaaaccttt
     6421 acccattacc agccgctggg caatagtgac ccggctcata ccgcaaccgc gcccggcgga
     6481 ttgagtgcga aagcgcctgc aatgaccccg ctgatgctgg acacctccac ccgtaagctg
     6541 gttgcgtggg atggcaccac cgacggtgct gccgttggca ttcttgcggt tgctgctgac
     6601 cagaccagca ccacactgac gttctacaag tccggcacgt tccgttatga ggatgtgctc
     6661 tggccggagg ctgccagcga cgagacgaaa aaacggaccg tgtttgccgg aacggcaatc
     6721 agcatcgttt aactttaccc ttcatcacta aaggccgcct gtgtggcttt ttttacggga
     6781 tttttttatg tcgatgtaca caaccgccca gctgctggcg gcaaatgagc agaaatttaa
     6841 gtttgatccg ctgtttctgc gtctcttttt ccgtgagagc tatcccttca ctacggagaa
     6901 agtctatctc tcacaaattc cgggactggt aaacatggcg ctgtacgttt cgccgattgt
     6961 ttccggtgag gttatccgtt cccgtggcgg ctccacctct gaatttacgc cgggatatgt
     7021 caaacccaag catgaggtga atccgcagat gaccctgcgt cgcctgccgg atgaagatcc
     7081 gcagaatctg gcggacccgg cttaccgccg ccgtcgcatc atcatgcaga acatgcgtga
     7141 cgaagagctg gccattgctc aggtcgaaga gatgcaggca gtttctgccg tgctcaaggg
     7201 caaatacacc atgaccggtg aagccttcga tccggttgag gtggatatgg gccgcagtgc
     7261 ggcgaacaac atcacacagt ccggcgtcac ggagtggagc aagcgtgaca agtccacgta
     7321 tgacccgacc gacgatatcg aagcctacgc gctgaacgcc agcggtgtgg tgaatatcat
     7381 cgtgtttgac ccgaaaggct gggcgctgtt ccgttccttc aaagccgtca aggagaagct
     7441 ggatacccgt cgcggctctc attccgagct ggagacagcg gtaaaagacc tgggcaaagc
     7501 ggtgtcctat aagggaatgt atggcgatgt ggccatcgtc gtgtattccg gacagtacgt
     7561 ggaaaacggc gtcaaaaaga acttcctgcc ggacaacacg atggtgctgg ggaacactca
     7621 ggctcgcggt ctgcgcacct atggctgcat tcaggatgcg gacgcacagc gcgaaggcat
     7681 taacgcttct gcccgttacc cgaaaaactg ggtgaccacc ggcgatccgg cgcgtgagtt
     7741 caccatgatt cagtcagcac cgctgatgct gctggctgat cctgatgagt tcgtgtccgt
     7801 tcaactggcg taatcatggc ccttcggggc cattttctct ctgtggagga gttcatgacg
     7861 aaagatgaac tgattgcccg tcttcaggag ctgggtgagc aattgaaccg cgatgtcagt
     7921 ttgacgggaa cgaaagaaga actggcgctt cgtgtggcag agctggaaga agagcttgat
     7981 gacacggatg aaactgccgg tcaggacacc cctctcagcc gggaaaatgt gctgaccggg
     8041 catgaaaatg aggtggtatc agcgcagccg gataccgtga ttcaggatac ggctgaactg
     8101 gtcacggtcg tggcactggt gacgctgcat actgatgcac ttcacgccac gcgggataaa
     8161 cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct ctgccggtgt ggcagccgaa
     8221 atgacagagc gcggcctggc cagaatgcaa taacgggagg cgctgtggct gatttcgata
     8281 acctgttcga tgctgccatt gcccgcgccg atgaaacgat acgcgggtac atgggaacgt
     8341 cagccaccat gacatccggt gagcagtcag gtgcggtgat acgtggtgtt tttgatgacc
     8401 ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga aggctccagc ccgtccctgt
     8461 ttgtccggac tgatgaggtg cggcagctgc ggcgtggaga cacgctgacc atcggtgagg
     8521 aaaatttctg ggtagatcgg gtttcgccgg atgatggcgg aagttgtcat ctctggcttg
     8581 gacggggcgt accgcctgcc gttaaccgtc gccgctgaaa gggggatgta tggccataaa
     8641 aggtcttgag caggccgttg aaaacctcag ccgtatcagc aaaacggcgg tgcccggtgc
     8701 cgccgcaatg gccattaacc gcgttgcttc atccgcgata tcgcagtctg cgtcacaggt
     8761 tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa agggccaggc tgaaaagggc
     8821 cacggtcaaa aatccgcagg ccagaatcaa agttaaccgg ggggatttgc ccgtaatcaa
     8881 gctgggtaat gcgcgggttg tcctgtcccg ccgcaggcgt cataaaaagg ggcagcgttc
     8941 atccctgaaa ggtggcggca gcgtgcttgt ggtgggtaac cgtcgtattc ccggcgcgtt
     9001 tattcagcaa ctgaaaaacg gccggtggca tgttatgcag cgtgtggccg ggaaaaaccg
     9061 ttaccccatt gatgtggtga aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca
     9121 gaatattgaa cggatacggc gtgaacgtct tccgaaagag ctgggctatg cgctgcagca
     9181 tcaactgaga atggtaataa agcgatgaaa catactgaac tccgtgcagc cgtactggat
     9241 gcactggaga agcatgacac cggggcgacg ctttttgatg gtcgccccgc tgtttttgat
     9301 gaggaagatt ttccggcaat tgccgtttat ctcaccggcg ctgaatacac gggcgaagag
     9361 ctggacagcg atacctggca ggcggagctg catattgaag ttttcctgcc tgctcaggtg
     9421 ccggattcag agctggattc gtggatggag tcccggattt atccggtgat gagcgatatc
     9481 ccggcactgt cagatttgat caccagtatg gtggccagcg gctatgacta ccggcgcgac
     9541 gatgatgcgg gcctgtggag ttcagccgat ctgacttatg tcattatcta tgaaatgtga
     9601 ggacgctatg cctgtaccaa atcctacaat gccggtgaaa ggtgccggga ccaccctgtg
     9661 ggtttataag gggagcggtg acccttatgc gaacccgctt tcagacgttg actggtcgcg
     9721 tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag
     9781 ctatctcgat gatgaagatg cagactggac tgcgaccggg caggggcaga aatctgccgg
     9841 agataccagc ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc
     9901 gtggtttaat gaaggtgata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga
     9961 tgtgttccgt ggctgggtca gcagtatcgg taaggcggtg acggcgaagg aggtgattac
    10021 ccgcacggtg aaggtcacca atgtgggacg tccgtcgatg gcagaagatc gcagcacggt
    10081 gacggcgacg accggcatga cggtgacacc cgccagtgct tccgtagtga aagggcagag
    10141 caccacgctg accgtggcat tccagccgga tggcgcaacc gacaagagct tccgtgcggt
    10201 gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaaagg
    10261 tgttgctgca ggcaaggtca acattccggt cgtatctggt aatggtgagt ttgctgcggt
    10321 tgcagaaatc aacgtcaccg ccagttaatc cggagagtca gcgatgttcc tgaaaaccga
    10381 atcatttgaa cataacggtg tgaccgtcac gctttctgaa ctgtcagccc tgcagcgtat
    10441 tgagcatctc gacctgatga aacggcaggc agaacaggcg gagtcagaca gcaaccggaa
    10501 gtttactgtg gaagacgcca tcagaaccgg cgcttttgtg gttgcgatgt ccctgtggca
    10561 taaccatccg aagaagacgc agatgccgtc catgaatgaa gccgttaaac agattgagca
    10621 ggaagtgctt accacctggc ccacagaggc aatttctcat gctgaaaacg tggtgtaccg
    10681 gctgtccggt atgtatgagt ttgtggtgaa tgatgctcct gaacaggcag aggacgccgg
    10741 gcctgcagag cctgtttctg cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa
    10801 ctggcgcgtg agatggggcg acccgactgg cgcgccatgc ttgccgggat gtcatccacg
    10861 gagtatgccg actggcaccg cttttacagt acccattatt ttcatgatgt tctgctggat
    10921 atgcactttt ccgggctgac gtacaccgtg ctcagcctgt ttttcagcga tccggagatg
    10981 catccgctgg atttcagtct gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat
    11041 gtgctgatgc agaaagcggc agggcttgcc ggaggtgtcc gctttggccc ggacgggaat
    11101 gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg
    11161 acagtatcag aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt
    11221 cgttgatttg agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca
    11281 tttttccggt acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag
    11341 ccgacaggcg ctggctgcac agaaagcggg gatttccgtc gggcagtata aagccgccat
    11401 gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag cttgcagggg ggcagaatcc
    11461 ctggctgatc ctgctgcaac agggtggtca ggttaaggac tccttcggcg ggatgatccc
    11521 catgttcagg gggcttgccg gcgcgatcac cctgccgatg gtgggggcca cctcgctggc
    11581 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    11641 caacaaaacg ctggtccttt ccggtaatca ggcgggactg acggcagatc gtatgctggt
    11701 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
    11761 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    11821 ggcgcgtttc tcctatgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    11881 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
    11941 ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
    12001 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
    12061 gaacatgggc acgctggaga cctgggcaga caggactgca cgtgcattca aatccatgtg
    12121 ggatgcggtg ctggatattg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
    12181 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt atttcgttaa
    12241 cgatgaagca cgggcgcgtt actgggatga tcgtgaaaaa aaacgcctgg agcgggatgc
    12301 agcccaaaag agggtagatc aacagcgtca acaggacaaa aatgcgcagc agcagagcga
    12361 taccgaagcg tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca
    12421 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    12481 aaaaatcctg caggcagatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    12541 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtat gcgggcgatc gtcaggaaga
    12601 cagtgctcat gcggccctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc
    12661 cggagcgaat gagaaaatca gccagcagcg ccgggatttg tggaaggcag aaagtcagtt
    12721 cgcggtactg gaggaggcgg cacaacgtcg ccagctgtcc gcacaggaga aatccctgct
    12781 ggcgcataaa gacgagacgc tggagtacaa acgccagctg gctgcacttg gtgacaaggt
    12841 tacgtatcag gagcacctga atgcgctggc gcagcaggcg gataaattcg cacagcagca
    12901 acgggcaaaa cgggcagcca ttgatgcgaa aaaccggggg ctgactgacc ggcaggcagc
    12961 gcgggaagcc acggaacagc gcctgaagga acagtatggc gataatcctc tggcgctgaa
    13021 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggagctg
    13081 gatggcaggc ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    13141 acaggttaaa agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcggcgat
    13201 gctgaccggc agtgaacaga actggcgcag cttcacccgc tccgtgctgt ccatgatgac
    13261 agaaattctg cttaagcagg caatggtggg gattgtcggg agtatcggca gcgccattgg
    13321 cggggctgtt ggtggcggcg catccgcgtc aggtggtaca gccattcagg ccgctgcggt
    13381 gaaattccat tttgcaaccg ggggatttac gggaaccggc ggcaaatatg agccagcggg
    13441 gattgttcac cgtggtgaat ttgtcttcac gaaggaggca accagccgga ttggcgtggg
    13501 gaatctctac cggctgatgc gcggctatgc ggaaggtggt tatgtgggcg gtgccggaag
    13561 tccggcgcag atgcggcggg ctgaaggcat taattttaat cagaacaatc acgtggtgat
    13621 tcagaacgac ggtacgaatg gtctgccagg tccacagatg atgaaggccg tgtatgacat
    13681 ggcccgcaag ggtgcccgtg atgaaattca gacacagatg cgtgatggtg gcctgttctc
    13741 cggaggtgga cgatgaaaac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg
    13801 gccccttctg taagaaaggt gcgctttggt gatggctatt cccagcgagc gcctgccggg
    13861 ctgaatgcca acctgaaaac gtacagcgtg acgctttctg tcccccgtga ggaggccatg
    13921 gtactggagt cgtttctgga agagcacggg ggctggaaag cctttctgtg gacgccgcct
    13981 tatgagtggc ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg
    14041 cgtgttgagt tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg
    14101 aaacactgaa tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg
    14161 atctgacaga ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg
    14221 agccggtcac ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg
    14281 aactgaatgg caaaggcacc agtacgcgcc ccacgctgat ggtttctaac ctgtacggta
    14341 tggtcaccgg gatggcggaa gatctacaga gtctggtcgg cggaacggtg gtccggcgta
    14401 aggtttacgc ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc
    14461 cggagcagga ggtgatcagc cgctggcgca ttgagcagtg cagcgaactg agcgcggtga
    14521 gtgcctcttt tgtactgtcc acgccgacgg aaacagatgg cgctgttttt ccgggacgta
    14581 tcatgctggc caacacctgc acctggacct atcgcggcga tgagtgcggt tatcacggtc
    14641 cggcggtcgc ggatgaatat gaccagccaa cgtccgacat cacgaaggat aaatgcagca
    14701 aatgcctgag tggctgtaag tttcgcaata acgtcggcaa ctttggcggc ttcctttcca
    14761 ttaacaaact ttcgcagtaa atcccatgac agagacagaa tcagcgattc tggcgcacgc
    14821 ccggcgatgt gctccagcgg agtcgtgcgg cttcgtggta agaacgccgg agggggaaag
    14881 atatttcccc tgcgtgaata tctccggtga gccggaggcg tatttccgta tgtcgccgga
    14941 agactggctg caggcagaga tgcagggtga gattgtggca ctggtccaca gccaccccgg
    15001 tggtctgccc tggctgagtg aggccgaccg gcggctgcag gtgcagagtg atttgccgtg
    15061 gtggctggtc tgccgggggg cgattcacaa gttccgctgt gtgccacatc ttaccgggcg
    15121 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt accatctggc
    15181 ggggattgag atgccggatt ttcatcgtga ggatgactgg tggcgtcacg gtcagaatct
    15241 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcag cggcgcagcc
    15301 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
    15361 tggtgacggc gagctgctgc accatattcc tgaacaactg agcaaacgag agaggtatac
    15421 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggcatggc acgcatctgc
    15481 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggtgcc
    15541 gaagccatcc gggcgatggc cacacagctc cctgcgtttc gtcagaagct gagcgacggc
    15601 tggtatcagg tgcgtattgc cgggcaggat gttagcacgt ccggattaac ggcacagtta
    15661 catgaggctc tgcctgacgg cgctgtgatt catattgttc ccagagtcgc cggggccaag
    15721 tcaggtggtg tattccagat tgtcctggga gcagccgcca ttgccggatc attctttacc
    15781 gccggagcca cccttgcagc atggggggct gccattggtg ccggtggtat gaccggcatt
    15841 ctgttttctc tcggtgccag tatggtgctc ggcggtgtgg ctcagatgct ggcaccgaaa
    15901 gccagaactc cccgcacaca gacaacggat aacggtaagc agaacaccta tttctcctca
    15961 ctggataaca tggttgccca gggcaatgtt atgcctgttc tgtatggtga aatgcgcgtg
    16021 gggtcacgtg tggtatctca ggagatcagc acggcagacg aaggggacgg tggtcaggtt
    16081 gtggtgattg gtcgctgatg caaaatgttt tatgtgaaat cgcctccggg cggttttatc
    16141 gtttatggag catgacgaat gggtaaaggc agcagtaagg ggcatacccc gcgcgaagcg
    16201 aaggacaacc tgaagtccac gcagctgctg agtgtgatcg atgccatcag cgaagggccg
    16261 gttgaaggtc cggtggatgg attaaaaagc gtgctgctga acagtacgcc ggtgctggac
    16321 agtgagggga ataccaacat ctccggcgtc acggtggtgt tccgggcagg tgagcaggag
    16381 cagtcaccgc cggagggatt tgaatcctcc ggctccgaga cggtgctggg tacggaagtg
    16441 aaatatgaca cgccgatcac ccggaccatc acgtcggcaa acattgaccg actgcgtttt
    16501 accttcggtg tacaggcact ggtggaaacc acctcaaagg gagacaggaa tccgtctgaa
    16561 gttcgcctgc tggttcagat acagcgtaac ggtggctggg tgacggaaaa agacatcacc
    16621 attaagggca aaaccacctc gcagtatctg gcctcggtgg tggtggataa cctgccgccg
    16681 cgcccgttta atatccggat gcgcagaatg acgccggaca gcaccacaga ccagctgcag
    16741 aacaaaacgc tctggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac
    16801 acggcactgg tcggcgtaca ggtggattcg gagcagttcg gcagccagca ggtgagccgt
    16861 aattatcatc ttcgcgggcg cattctgcag gtgccgtcga actataatcc gcagacgcgg
    16921 caatacagcg gtatctggga cggaacgtta aaaccggcat acagcaacaa catggcctgg
    16981 tgtctgtggg atatgctgac ccatccgcgc tatggcatgg ggaaacgtct tggtgcggca
    17041 gatgtggata aatgggcgct gtatgtcatc ggccagtact gtgaccagtc ggtgccggac
    17101 ggctttggca gcacggagcc gcgcatcacc tgtaatgcct ggctgaccac acagcgcaag
    17161 gcgtgggatg tgctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggg
    17221 cagacgctga cgttcgtgca ggaccgacag tcggataagg tgtgggccta taaccgcagt
    17281 aatgtggtga tgccggatga tggcgcgccg ttccgctaca gtttcagcgc cctgaaagac
    17341 cgccataatg ccgttgaggt gaactggatt gacccggata acggctggga gacggcgaca
    17401 gagcttgtgg aggatacgca ggccattgtc cgttacggtc gtaacgtcac gaagatggat
    17461 gcctttggct gtaccagccg ggggcaggca caccgcgccg ggctgtggct gattaaaacg
    17521 gaactgctgg agacgcagac cgtggatttc agcgtgggtg ctgaagggct tcgtcatgta
    17581 ccgggcgatg tcattgaaat ctgcgatgat gactatgccg gtatccgcac cggcgggcgc
    17641 gtgctggcgg tgaacagcca gacccggacg ctgacgctcg accgtgaaat cacgctgcca
    17701 tcctccggca ccacgctgat aagcctggtt gacgggcagg ggaatccggt cagcgtggag
    17761 gtccagtccg tcaccgacgg cgtgaaggta aaagtgagcc gtgttcctga cggtgttgct
    17821 gaatacagcg tgtgggggct gaagctgccg acgctgcacc agcgattgtt ccgctgcgtg
    17881 agtatccgtg agaacgacga cggcacgtat gccatcaccg ccgtgcagca tgtgccggaa
    17941 aaagaagcca tcgtggataa cggggcgcac tttgacggcg accagagcgg cacggtgaat
    18001 ggtgtcacgc cgccagcggt gcagcacctg actgccgaag tcaccgcaga cagcggggaa
    18061 tatcaggtgc tggcgcgctg ggacacgccg aaggtggtga agggcgtgag cttcctgctc
    18121 cgtctgaccg taacagcgga tgacggcagt gagcggctgg tcagcacggc ccggacgacg
    18181 gaaaccactt accgcttcac acaactggcg ctggggaact acaggctgac agtccgggca
    18241 gtaaatgcgt gggggcagca gggcgatccg gcgtcggtat cgttccggat tgccgcaccg
    18301 gcagcgctgt cgcggattga gctgacgccg ggctattttc agataaccgc cacgccgcat
    18361 cttgcggttt atgatccgac ggtacagttt gagttctggt tctcggaaaa gcggattgcg
    18421 gatatcaggc aggttgaaac cacagcacgc tatcttggca cggcgctgta ctggatagcc
    18481 gccagtatca atatcaaacc gggccatgat tattactttt atatccgcag tgtgaacacc
    18541 gttggcaaat cggcattcgt ggaggctgtt ggtcagccga gtgatgatgc atccggttat
    18601 ctggattttt tcaaaggcga gatagggaaa acccatctgg ctcaggagct gtggacgcag
    18661 attgataacg gtcagcttgc gcctgacctg gctgaaatca ggacgtccat tacggatgtc
    18721 agcaatgaaa tcacgcagac cgtcaataag aaactggaag accagagtgc ggcaattcag
    18781 cagatacaga aggttcaggt tgatacaaat aataacctga acagcatgtg ggctgtgaag
    18841 ctgcagcaga tgcaggacgg acgcctttat atcgcgggta ttggtgccgg tattgagaac
    18901 acccctgacg gcatgcagag tcaggtgctg ctggcggcgg acaggattgc gatggttaat
    18961 cctgcgaatg gcaacacaaa accgatgttt gttggtcagg gcgatcagat attcatgaac
    19021 gacgtgttcc tgaaacgcct gacggccccc accattacca gcggtggaaa tccatcggca
    19081 ttttccctga ctccggacgg aaagctgacc gctaaaaatg cggatatcag tggcagtgtg
    19141 aatgcgaacg ccgggacgct caacaatgtc acggtaaatg aaaactgtac gattaagggc
    19201 atgctggagg cgactcaggt cagaggtgac ttcgttaaag ctgtatccaa atcatttccg
    19261 aaacaggctg gtacgtgggg taacacggaa acaccaaacg ggacggttac agtcaccata
    19321 agcgatgatc ataactttga ccgccagatt attattccgc ccattatttt taacggtata
    19381 gcgtatgacg atccggggag cggaaataac ccaggaggca cgcgatacac gggttatggt
    19441 tttgaagttc gcaaaaacgg cgtattaatc gcatccagag aaactaaagg tgccattccc
    19501 ggtagttaca gtgcagttat tgatatgcct agtggtggtg gtagcgtcac tctggagttt
    19561 aagattttcc agaaaggcaa tcagggggca ggcaatatca ccgactgtac ggtgattgtg
    19621 accaaaaaag cggcttccgg catcagtatt cgttgaaata tttataaccc caataaatgg
    19681 cgtcaggaat gacgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaagtttg
    19741 tgcagccatt ttgtccgcag ccatttgcct ggccgtatcc ggtgcgcctg catgggcgtc
    19801 tgaacatcag tccacgctga gcgcggggta tcttcatgcc cggaccaacg ttcccggcag
    19861 tgatgatctg aacgggatta acgtgaaata ccgttatgag tttacggata cgctggggct
    19921 ggtgacgtca ttcagctatg caggagacaa gaatcgccag ctgacccgtt acagcgatac
    19981 ccgctggcat gaagattccg tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt
    20041 gcgcgtgaat gaatggttca gcgcgtatgc gatggcgggt gtggcttaca gccgtgtgtc
    20101 gactttttcc ggggattata tccgcgtaac tgacaataag gggaaaacgc acgatgtgct
    20161 gaccggaagt gatgacggtc gccacagcaa cacgtctctg gcgcactgac ggtttcatcg
    20221 tgggtgtcgg ttataaattc tgattagcca ggtaacacag tgttatgaca gcccgccggt
    20281 tcaggcgggc ttttttgtgg ggtgaatatg gcagtaaaga tttcaggtgt actgaaagac
    20341 ggcacaggaa aaccggtaca gaactgcaca atccagctga aagcaaaacg taacagcacc
    20401 acggtggtgg tgaacacgct ggcctcagaa aatccggatg aagccgggcg ttacagcatg
    20461 gacgttgagt acggtcagta cagcgttatt ctgttggtgg aaggattccc gccgtcacat
    20521 gccgggacca tcaccgtgta tgaagattcc cgacccggta cgctgaatga ttttctcggt
    20581 gccatgacgg aggatgatgc ccgtccggag gcactgcgcc gttttgagct gatggtggaa
    20641 gaggtggcgc gtaacgcgtc cgcggtggca cagaacacgg cagccgcgaa gaaatcagcc
    20701 agcgatgcca gcacatcagc ccgtgaggcg gcaacccatg cgactgatgc tgcagattca
    20761 gcacgtgcag ccagcacgtc agccggacag gccgcttcgt cggctcagtc agcgtcttcc
    20821 agcgcaggaa cggcatcaac aaaggccact gaagcctcaa aaagtgctgc cgctgcagag
    20881 tcctcaaaaa gcgcggcggc taccagtgcc ggtgcggcga aaacgttaga aacgaatgcg
    20941 gcagcgtcac aacaatcagc agccacttct gcatccaccg cgaccacgaa agcgtcagaa
    21001 gctgccacct cagcccggga tgcgtcggct tcaaaagagg cggcaaaatc atcagaaacg
    21061 aacgcatcat caagcgccag tagcgcagct tcctcggcga cggcggctgc aaattctgcg
    21121 aaggcggcca aaacgtccga gacgaacgcc aggtcttctg aaacggcagc gggacagagt
    21181 gcctcagctg cggcaggctc aaaaacagcg gctgcgtcgt ctgccagcgc cgcgtcaaca
    21241 agtgccgggc aggcctcagc cagtgccacc gccgccggaa aatcggcaga aagcgccgca
    21301 tcatccgctt caacagccac aacgaaggct ggcgaagcca ctgagcaggc cacggcagca
    21361 gcgaggtcag cttccgcagc gaagacatcc gaaacgaacg cgaaagcgtc ggaaacccgt
    21421 gcagaatcct caaaaacggc tgccgcatcg tccgccagtt cggcggcgtc atcggcatca
    21481 tcggcgtctg cttcaaaaga tgaggcgacc agacaggcgt cagcagcgaa gggcagcgcc
    21541 acgacggcat ccacgaaggc gacagaggct gctggtagtg cgacggcagc agctcagagc
    21601 aaaagtacgg cggaatctgc agcaacgcgc gctgagacag cggcaaaacg ggcagaggat
    21661 attgcatccg ccgtggcgct tgaggatgcg agcacgacga aaaaggggat agtacagctc
    21721 agtagtgcga ctaacagcac ttccgagtca ctggcggcaa caccaaaagc cgttaaggcc
    21781 gcgtatgacc tggctaacgg gaaatacacc gcacaggatg caacgacagc acagaaaggg
    21841 ataatccagc taagcagcgc gaccaacagc acgtctgaaa cgctggcggc aacgccaaag
    21901 gcagtaaaag cagccaatga caatgctgag aaacgtctgc agaaagatca gaacggtgcg
    21961 gatatccctg gcaaagacac ctttacgaaa aatattggtg cctgccgtgc cttcggtggg
    22021 tcagtaagca caacaacagg aaactggacg actgcacagt ttatcgagtg gctggattct
    22081 cagggagcat ttaaccatcc atactggatg tgcaagggtt cctggtctta tggcaataat
    22141 aaaatcatta ctgatactgg ctgcggtaat attcatctcg ccggagctgt cattgaagta
    22201 atggggataa agtcagcgat gacgatccgc attaccactc cgaccacatc cagcggtggc
    22261 ggaacaacca acgcccagtt tacctatatt aatcacggaa cagattattc acctggctgg
    22321 cgaagggact ataactccag aaataagcca acggcatcag agatcggggc gctaccgtca
    22381 ggtggaacag cagtatcatc agttaatctg gcttcaaaag gtcgggtagc cgcgctgaca
    22441 gacaatacgc agggggcagc aggtcttgag ttatacgagg tgtataacaa cggatatcca
    22501 acagcgtatg gaaatatcat tcacctgaaa gggatgacag ccgttggcga aggtgagtta
    22561 ctcatcggct ggagtggtac aagcggtgct catgctccgg catttattcg ttcacgacgg
    22621 gatacgaccg acgcaaactg gtcgccgtgg gcgcagcttt acacctcggc tcatcctcct
    22681 gcagagtttt atccagtcgg tgcaccaatc ccgtggccat cagataccgt tccgtctggt
    22741 tatgccctga tgcaggggca gacttttgac aaatctgctt acccgaaact tgcagccgct
    22801 tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa gcccgccagt
    22861 ggtcgtgccg tattgtctca ggaacaggat ggcattaaat cgcacaccca cagcgccagc
    22921 gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg cactaaatcc
    22981 acgaataaca ccggggcgca tacgcacagt ctgagcggct ctacaagttc agctggggcc
    23041 caccaacata gtcagactgg gcccagaacg aatagcggga gtcaacctac tggaatgttt
    23101 ccggcaggga gtactcaagt tagcgggact aatcaggttg gtatcagtgg ctcattaacc
    23161 agtggcacct cccaatgggt aggtaaatca tcttcagaag gaaaccatac acactcactg
    23221 tccggcacag cagcatctgc gggtgcacac gcgcatactg tcggtattgg tgctcatacg
    23281 cactctgttg cgattggttc acatggacac accatcaccg ttaacgctgc tggtaacgcg
    23341 gaaaacaccg tcaaaaacat cgcatttaac tatattgtga ggctcgcata atggcattca
    23401 gaatgagtga acaagcacgg accataaaaa tttataacct gctggccgga actaatgaat
    23461 ttattggtga aggtgacgca tatattccgc ctcatacagg tctgcctgca aacagtaccg
    23521 atattgcacc gccagatatt ccggctggtt tcgtggctgt tttcaacagt gatgagtcat
    23581 cgtggcatct cgttgaagat catcggggta aaacggttta tgacgtggct tccggcgacg
    23641 cgttatttat ttctgaactc ggctcattac cggaaaatgt cacctggtta tccccggaag
    23701 gggagtttca gaagtggaac ggcacagcct gggtgaagga tacggaagca gaaaaactgt
    23761 tccggatccg ggaggcggaa gaaacaaaaa acagcctgat gcaggtagcc agtgagcata
    23821 tagcgccgct tcaggatgct gtggatctgg agatcgcaac ggagaaagaa accttgttgc
    23881 tggaagcttg gaaaaagtat cgggtgttgc tgaaccgtgt tgatacatca actg
//
LOCUS       DAZOXR010000053        23767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZOXR010000053 DAZOXR010000000
VERSION     DAZOXR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.053"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(366..1163)
                     /locus_tag="ACHUNN_001797"
     CDS             complement(366..1163)
                     /locus_tag="ACHUNN_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0576192.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1173..1724)
                     /gene="ybcL"
                     /locus_tag="ACHUNN_001798"
     CDS             complement(1173..1724)
                     /gene="ybcL"
                     /locus_tag="ACHUNN_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0576193.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1893..2225)
                     /gene="emrE"
                     /locus_tag="ACHUNN_001799"
     CDS             complement(1893..2225)
                     /gene="emrE"
                     /locus_tag="ACHUNN_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0576194.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            2406..2534
                     /locus_tag="ACHUNN_001800"
                     /pseudo
     CDS             2406..2534
                     /locus_tag="ACHUNN_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2559..2873)
                     /gene="fliE"
                     /locus_tag="ACHUNN_001801"
     CDS             complement(2559..2873)
                     /gene="fliE"
                     /locus_tag="ACHUNN_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0576195.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3089..4747
                     /gene="fliF"
                     /locus_tag="ACHUNN_001802"
     CDS             3089..4747
                     /gene="fliF"
                     /locus_tag="ACHUNN_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR0576196.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4740..5735
                     /gene="fliG"
                     /locus_tag="ACHUNN_001803"
     CDS             4740..5735
                     /gene="fliG"
                     /locus_tag="ACHUNN_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0576197.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5728..6414
                     /gene="fliH"
                     /locus_tag="ACHUNN_001804"
     CDS             5728..6414
                     /gene="fliH"
                     /locus_tag="ACHUNN_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0576198.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6414..7787
                     /gene="fliI"
                     /locus_tag="ACHUNN_001805"
     CDS             6414..7787
                     /gene="fliI"
                     /locus_tag="ACHUNN_001805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0576199.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7806..8249
                     /gene="fliJ"
                     /locus_tag="ACHUNN_001806"
     CDS             7806..8249
                     /gene="fliJ"
                     /locus_tag="ACHUNN_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR0576200.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8246..9373
                     /locus_tag="ACHUNN_001807"
     CDS             8246..9373
                     /locus_tag="ACHUNN_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0576201.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9478..9942
                     /gene="fliL"
                     /locus_tag="ACHUNN_001808"
     CDS             9478..9942
                     /gene="fliL"
                     /locus_tag="ACHUNN_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0576202.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9947..10951
                     /gene="fliM"
                     /locus_tag="ACHUNN_001809"
     CDS             9947..10951
                     /gene="fliM"
                     /locus_tag="ACHUNN_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0576203.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10948..11361
                     /gene="fliN"
                     /locus_tag="ACHUNN_001810"
     CDS             10948..11361
                     /gene="fliN"
                     /locus_tag="ACHUNN_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0576204.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11364..11729
                     /gene="fliO"
                     /locus_tag="ACHUNN_001811"
     CDS             11364..11729
                     /gene="fliO"
                     /locus_tag="ACHUNN_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0576205.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11729..12466
                     /gene="fliP"
                     /locus_tag="ACHUNN_001812"
     CDS             11729..12466
                     /gene="fliP"
                     /locus_tag="ACHUNN_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR0576206.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12476..12745
                     /gene="fliQ"
                     /locus_tag="ACHUNN_001813"
     CDS             12476..12745
                     /gene="fliQ"
                     /locus_tag="ACHUNN_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0576207.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12754..13539
                     /gene="fliR"
                     /locus_tag="ACHUNN_001814"
     CDS             12754..13539
                     /gene="fliR"
                     /locus_tag="ACHUNN_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0576208.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13824..14447
                     /gene="rcsA"
                     /locus_tag="ACHUNN_001815"
     CDS             13824..14447
                     /gene="rcsA"
                     /locus_tag="ACHUNN_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR0576209.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14491..14679)
                     /gene="dsrB"
                     /locus_tag="ACHUNN_001816"
     CDS             complement(14491..14679)
                     /gene="dsrB"
                     /locus_tag="ACHUNN_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0576210.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14842..15069
                     /gene="yodD"
                     /locus_tag="ACHUNN_001817"
     CDS             14842..15069
                     /gene="yodD"
                     /locus_tag="ACHUNN_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0576211.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15367..16182
                     /locus_tag="ACHUNN_001818"
     CDS             15367..16182
                     /locus_tag="ACHUNN_001818"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR0576212.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16179..17873)
                     /gene="dgcQ"
                     /locus_tag="ACHUNN_001819"
     CDS             complement(16179..17873)
                     /gene="dgcQ"
                     /locus_tag="ACHUNN_001819"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR0576213.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18044..18226)
                     /locus_tag="ACHUNN_001820"
     CDS             complement(18044..18226)
                     /locus_tag="ACHUNN_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0576214.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18305..19222)
                     /locus_tag="ACHUNN_001821"
     CDS             complement(18305..19222)
                     /locus_tag="ACHUNN_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0576215.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19395..20316
                     /gene="yedA"
                     /locus_tag="ACHUNN_001822"
                     /pseudo
     CDS             19395..20316
                     /gene="yedA"
                     /locus_tag="ACHUNN_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20305..20775)
                     /gene="vsr"
                     /locus_tag="ACHUNN_001823"
     CDS             complement(20305..20775)
                     /gene="vsr"
                     /locus_tag="ACHUNN_001823"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0576216.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20756..22174)
                     /gene="dcm"
                     /locus_tag="ACHUNN_001824"
     CDS             complement(20756..22174)
                     /gene="dcm"
                     /locus_tag="ACHUNN_001824"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0576217.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22241..22897)
                     /locus_tag="ACHUNN_001825"
     CDS             complement(22241..22897)
                     /locus_tag="ACHUNN_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR0576218.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(22937..23218)
                     /gene="drpB"
                     /locus_tag="ACHUNN_001826"
     CDS             complement(22937..23218)
                     /gene="drpB"
                     /locus_tag="ACHUNN_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0576219.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5909 a         6157 c         5898 g         5803 t
ORIGIN      
        1 ggcaatttct gctgtagctg catcttaacg tataaactag atttaataat aacgaactag
       61 atttaataac aacgatatcc gtacctatcc ggatatctaa tgtcataagc atccctttaa
      121 ttatcccctc aacctcttgt aactttccca ctcttgaact ccacatagtc caaacgccgg
      181 atgcaaaaaa atcacacgtg catttctgaa attgtgatgg aggaagatag tatcaattat
      241 atgtgcttat aaacgtcccc gattagcgca gaagtcaccg gattgttcag gctccaatag
      301 gttgagtatg ccgtatttat tttccaaaat aaaaatcgat gctgttgctc cttcataaaa
      361 tacttttact ctctggacgc ttttcttagc tctcttggtg ttatcttaaa acgattatgg
      421 aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat ctcgctgatg
      481 ggttttactg tcgtttgtat agcagacaac gcattattta acctcacgtc gtccaatata
      541 ctttgaaaac ttactccctc gcttgccaga cggcgatgca atgtggaaac agaaatgtag
      601 agatatctcg ctaccttgtt tgctgtccat tttgttccag gttcggatag cattaggtta
      661 taacaacgac ttattaatga ttgtttacta tctgataaaa gtaaacaatt aacatgatgt
      721 gctcctaatg aaagtagaac gcccattgct aagtgttcct gaatttgcgt tgagaagcct
      781 cgggaaatag atgtttttag ttgctcccaa caatatatta actcggaatt ctgaggtaag
      841 cagaaacctg tcgtgttacg aatttgatcg gttatcgcat aaagattttg gaacttttca
      901 attagatcaa tgggataata aagcatttct gcaagataaa gacctgcttc tgggtaattc
      961 gcaatataaa attcatatcc acagggaaat aatattatct gattattatc aacagttaga
     1021 ctatgcgtct cccaattgat aacttttttt ccctgacgga tacgacatga agctggcata
     1081 agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc gtatttcttc aatctttagg
     1141 ttagtcctat cttttgccag catactctta tcctacttta cctcataaac gggtattatc
     1201 tcagcggttg caattttatt ggcattaagc atataaccaa ctaacgctcc gctggaatta
     1261 gaatccacag gaatcttatc agtttttaga gcccatattt taaactggta atgatggggt
     1321 ttatctcctt tgggaggaca tgctccacca aatcccgcat agccaaaatc atttcggcct
     1381 tggacagcac cagtcggaag ttttgtacca tcacgtcttc ctgcatcagc gggcaaatat
     1441 gttaccgttg ctggaatatt agcaacagtc cagtgccacc atccgctgcc tgtaggcgca
     1501 tctggatcat acacagttac ggcaaagctt ttggtgcctt ctggagcacc agaccaggtt
     1561 aatgagggcg atgtattacc cccctcacac ccaactccag aaaatacatg agacgttgta
     1621 agttgctctc ctgtttttat ttcattacta gtgacctgaa atgctgcggc ctgcgcagaa
     1681 aatgttatga atgccaatac acttgaaacg ataagttttt tcataaaaac ctctttgttg
     1741 tgacctatcg ttattttatt tgatgtgcat tgatctcatt atgcaaaaag gcgcaatgat
     1801 cgcgcaaatg caatcataat tataaagttg ccccgaacat tagccataat atgcacaaat
     1861 cagattcctg ttatctcaag gatgattatt ttttaatgcg gtgcgcttcg tgacaacaag
     1921 ttaatgacta acacaccggc acaaatcaac atcatgccta tgatggctgg caggtccagt
     1981 cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa cgataccgac tcctgaccag
     2041 atagcataag caatccctgt aggtatataa gccagtgtct gagctaataa ccaaaatgat
     2101 gcacaatagc aaataattgt gccaacagag ggccataacc gtgtaaaccc ctctgaaaac
     2161 ttcattaagg ttgtaccaat gacctctgca agtattgcgc caccaagata aatataaggg
     2221 ttcatagcat attctttcct gttcatactg gagagaattg tactacagtt tgaacacaac
     2281 tcaccgtttt catcctggtg tatgtgtatc cagggggctc tggcgtggca agagataaag
     2341 gcaggaacaa taatgatcag aaggaacccc aggctaacaa aagagttcac tcttcctatg
     2401 agcaaagcga agacaaaaca tgttatcgcg tttacggatc gtggatggca gaaaactttc
     2461 aggatcaggt atccatttta aaccagaaat tgagtgagtt tgccccatcc atgccccacg
     2521 cggtgagatc ggatgtaatt aacaacaggt tacaaaacct acacctgcat ggacatcact
     2581 tcctgataag ccgccaccag cttattacgc acctgaatcc ccatttgcat agaaactgag
     2641 gctttttgca tatcggtcat cacatcgttt aacgccacgc cgggttcacc gagagtgaat
     2701 ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta tgcgatcgag cgcggcgtgc
     2761 agctgcccgg caaaactaat ggtcggttgt ggcagtgatt cctgcgcacg cgcactcatc
     2821 gccgtagcct gtaactggct gataacccct tcaatcccct gtatcgctga cattttcatc
     2881 tcccggataa tttctggtag caaagcttac cagtaagtca ataagacaaa ggcgctaaat
     2941 agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca aataatggca
     3001 gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt gccaataacg
     3061 ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga caaaatctct
     3121 tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg ccggttccgc
     3181 ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt
     3241 attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa
     3301 tattccttac tgcttcagcg aagccagcgg cgctattgaa gttccggcag ataaagttca
     3361 cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa ggcggcgcgg tcggtttcga
     3421 actgcttgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg
     3481 ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg
     3541 cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc
     3601 atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgccat
     3661 tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg ggaaacgtca cgctggtgga
     3721 tcagggcgga catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca
     3781 gttgaaatat gccagcgatg tcgaaggccg tattcagcgg cgtattgaag caatcctgtc
     3841 gcctattgtt ggtaacggta atattcacgc ccaggtcacg gcgcaactgg acttcgccag
     3901 taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg
     3961 ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg
     4021 ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccggcaaa
     4081 tcaaaataac cgccagcagc aggcgagcac caccagcaat agcgggccgc gtagcacaca
     4141 gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt
     4201 gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg
     4261 taaaccattg cctctcagca acgaacagat gaagcaaatt gaagatctga cccgcgaagc
     4321 gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc cgttcaatag
     4381 cagtgacgaa agcggcggag aactgccgtt ctggcaacag caagcgttta tcgatcagtt
     4441 gctggctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt ggcggaaagc
     4501 ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa gctgtacagc aacaggcgca
     4561 ggcccgtgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg aacaactcca
     4621 acaacgacgc gctaaccaac gtctgggggc agaagtcatg agccagcgta tccgtgaaat
     4681 gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa ataacgatca
     4741 tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt ggcgaagacc
     4801 gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa
     4861 tggcgaacgt cacgcagatc tccaacaagc agctaaccta tgtgctggcg gagtttgagc
     4921 aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg cgttcggtat
     4981 tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct ggaagatatt ctcgaaactc
     5041 gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagt gccgccgatc
     5101 tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg aagcgcgcgc
     5161 aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta
     5221 tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa gtactgaatg
     5281 gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg
     5341 aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc gtgcgcgaat
     5401 tcgacggcga gctggcgcaa aaaattatcg acgagatgtt cctgttcgaa aatctggtgg
     5461 atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa tcgctgttga
     5521 tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat atgtcgcagc
     5581 gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg
     5641 tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa actggcgaga
     5701 tggtaattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa aacctggacg
     5761 ccggacgatc tcgcgccacc acaggcagag tttgtgccca tggtcgagcc ggaagaaacc
     5821 atcattgaag aggccgaacc tagccttgag cagcaactgg cgcaactgca aatgcaggcc
     5881 catgagcaag gttatcaggc ggggattgcc gaaggtcgcc agcaaggtca tgagcagggc
     5941 tatcaggaag gactggccca ggggctggag caaggtctgg cagaggcgaa gtctcaacaa
     6001 gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac ccttgatgcg
     6061 cttgatagtg tgattgcttc gcgcctgatg cagatggcgc tggaggcggc acgtcaggtg
     6121 atcggtcaga cgccaacagt ggataactcg gcactgatca aacagatcca acagttgttg
     6181 cagcaagaac cgttattcag cggtaaacca cagctgcgcg tgcacccgga tgatctgcaa
     6241 cgtgtggatg atatgctcgg cgcaacctta agtttgcatg gctggcgctt gcggggcgat
     6301 cccacactcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct cgacgccagt
     6361 gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt gtaatgacca
     6421 cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg gcgcagttgc
     6481 ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg gctggtgctg gaagccaccg
     6541 gattacaatt gccgctcggc gcaacctgtg tcattgaacg ccagaacggc agcgaaacgc
     6601 acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta atgccgctgg
     6661 aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc caaaaacatt tcggctgaag
     6721 gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgc gttctggacg
     6781 gcagcggtaa gccgctcgat ggcctgccct cccccgatac gacggaaacc ggtgcgctga
     6841 ttaccccgcc atttaacccg ttgcaacgta caccgattga acatgtgctg gacaccggcg
     6901 tgcgcccaat caatgccctg cttaccgttg ggcgtgggca gcgtatgggg ctgtttgccg
     6961 ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc acgttacacc cgcgccgatg
     7021 tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt aaaagatttt attgagaata
     7081 tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc cgctccggcg gatgtctctc
     7141 cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat tgccgaagat tttcgcgatc
     7201 gtggtcagca tgtgttgctg attatggact ccctcacccg ctacgcgatg gcccagcgtg
     7261 aaattgcgct ggcgattggc gaaccacccg ccaccaaagg ttatccaccg tcggtgtttg
     7321 ccaaattacc ggcactggtc gagcgtgccg gaaatggcat tagcggcggc ggctcgatta
     7381 ccgcgtttta taccgtgctt actgaaggcg atgaccagca ggaccccatt gcagactccg
     7441 cacgggcgat cctcgacggt cacattgtgc tgtctcgccg actggcggaa gccgggcact
     7501 atccggctat cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg atcagtgagc
     7561 aacattacgc gcgagtgcgc accttcaaac agctgttgtc gagttttcag cgtaaccgcg
     7621 atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgat aaagccatcg
     7681 ccctgtggcc gcagctggag ggctatttac aacaaggcat ttttgaacgt gcggactggg
     7741 aagcgtctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc aggagataac
     7801 ggtagatggc agaacatggt gcgctggcga ccctgaaaga tctggcagaa aaagaggtag
     7861 aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg tcagcaggcg gaagaacagc
     7921 tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaacagc gatatgagtg
     7981 ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg ctggaaaaag
     8041 ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga
     8101 acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat
     8161 ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc
     8221 agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttgatta ccgccgacgt
     8281 tgacaccacc acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctcgcgtt
     8341 gttgagcgaa gcattagcaa gcgagacaac taccgacaaa gcggcccccc aggtgctggt
     8401 ggcaacagat aagcccacga caaaaggcga gctgctgatc agcgatattg tttccgacgc
     8461 gcaacaagcg gatttactga tccctgtgga tgaaacactg cctgtcatca acgtcgaaca
     8521 atccacatcg acaccgttaa ccaccgctca tacgatgacg ttggctgcgg tggctgacaa
     8581 aaatacgaca aaagacgaaa aagcggatga tctgaatgaa gacctcaccg caagcctgag
     8641 cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg atgcgccgtc
     8701 aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa ctgacttctg cgcaactcac
     8761 aacagcacag cctgatgatg cccccggcac gccagctcag ccattaacac cgctggtagc
     8821 agaagcccag agtaaagcgg aagtcatcag cacaccttca ccggtgaccg ctgccgccag
     8881 cccgctaatc actccacacc agacacagcc actgctcacc gtcgccgcgc ctgtcttgag
     8941 tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt cgctgttcac
     9001 ccgccaggga caacaaagtg cagagttgcg tctgcacccg caggatttag gtgaagtgca
     9061 aatctccctc aaagtggatg ataaccaggc gcaaatccag atggtttcgc cgcatcagca
     9121 cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt acgcagctgg ccgagagtgg
     9181 cattcagtta gggcaaagca acatcagtgg cgaaagcttt agtggtcagc agcaggccgc
     9241 ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa cctctggcgg gggaagaaga
     9301 cgatacgctt ccggttcccg tctctttaca agggcgcgta acaggcaaca gcggcgttga
     9361 tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg
     9421 ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg
     9481 actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggctcccgat tctggtattc
     9541 attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc
     9601 gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg tcttctatgc gctggatacc
     9661 tttacggtca atttgggcga tgcggatcgc gtgctttata tcggtataac cctgcgactc
     9721 aaagatgaag ctacccgctc gcggctgagt gagtatttgc cggaagtccg tagccgtttg
     9781 ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac
     9841 ctgattgccg agattaaaac cacgctttcc accccgcttg ttgccgggca accgaaacag
     9901 gatgtcaccg acgtgatgta taccgctttt attctgcgat aacgacatgg gcgatagtat
     9961 tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc
    10021 gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg
    10081 ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt
    10141 tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat ataaccgtcg gggccatccg
    10201 cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc tgaaccttat
    10261 ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt tcaccgagtc tggtgtttat
    10321 cgccgtggat aacctgtttg gcggcaatgg acgcttcccg accaaagtgg aaggtcgcga
    10381 gtttacccat accgaacagc gcgtcatcaa ccgcatgtta aagctggcgc ttgaaggcta
    10441 tagcgacgcc tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca
    10501 ggtgaaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca cgccgttcca
    10561 tgtggagatt ggcaacctga ccggcgaatt taatatctgt ctgccattca gcatgatcga
    10621 gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa
    10681 ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggagctgg tcgccaactt
    10741 tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc
    10801 gatagaaaaa cccgatggca tcatcgccca tgttgacggc gtcccggtgc tgaccagtca
    10861 gtacggcact ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt
    10921 aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac
    10981 aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc aaccagcagc
    11041 aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag cggaacgttg
    11101 caggatatcg acctgataat ggatattccg gtcaagctga ccgtcgagct gggccgtacg
    11161 cggatgacca tcaaagagct gttgcgtctg acgcaagggt ccgtcgtggc gctggacggt
    11221 ctggcgggcg aaccactgga tattctgatc aacggttatt taatctccca gggcgaagtg
    11281 gtggtcgttg ccgataaata tggcgtgcgg atcaccgata tcattactcc gtctgagcga
    11341 atgcgtcgcc tgagccgtta gtgatgaata accacgctac tgtgcaatct tccacgccgg
    11401 tttctgctgc gccactgctg caggtcagcg gcgcactcat cgccattatt gccctgatcc
    11461 tcgctgctgc ctggctggta aaacggttgg gatttgcccc gaaacgcact ggcgttaacg
    11521 gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggttgtg gtggtcgatg
    11581 tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctgcataaac
    11641 ttcccccttc agcaccaaca gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa
    11701 atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg ttgtctgtcg cacctgtcct
    11761 tctctggctg gttacgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct
    11821 gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt
    11881 gacgtttatt ccggcaattt tactgatgat gaccagtttc acccgcatca tcattgtttt
    11941 tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg tattgctggg
    12001 gctggcacta tttttgacct tttttattat gtcatcggtg atcgacaaaa tttatgtcga
    12061 tgcgtaccag ccattcagcg aagagaaaat atcaatgcag gaggcgctgg aaaaaggggc
    12121 gcagccgcta cgtgagttta tgctgcgtca gacccgtgag gcagatttag ggttgtttgc
    12181 cagactagcg aataccggcc cgttgcaggg acctgaagcc gtgccgatgc gcattttgct
    12241 cccggcctac gtgaccagcg aattgaaaac cgcatttcag ataggcttca cgattttcat
    12301 tccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat
    12361 ggtccccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg
    12421 ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac
    12481 acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg cactggctgc
    12541 cccgctgttg ctggtggcgc tggtcacggg ccttatcatc agtattttgc aggccgccac
    12601 gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc atcgccgtat ttattgccat
    12661 tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac
    12721 taacctgccg tatatcatcg ggtaggccgt acaatgatgc aggtgacaag cgatcaatgg
    12781 ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct catttctacc
    12841 gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactggggct ggcaatgatg
    12901 atcacgttcg tcattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt
    12961 gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt taccatgcaa
    13021 tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca
    13081 tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc ccgttttagc gcgtatcatg
    13141 gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt gatttcactg
    13201 ctggtcgata cctttcacac cctgccaatt ggtggcgaac cgttgaacag caatgcgttt
    13261 ctggcactca ccaaagcggg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg
    13321 ctcattactc tgctgctgac cctgaatctg gcattaggtt tactgaatcg tatggccccg
    13381 caattatcca tttttgttat tggatttcca ttaactctga ctgtcggtat ctctttaatg
    13441 gcggcattaa tgccgttaat tgcacctttt tgcgaacatt tattcagtga gatgtttaat
    13501 ttgctggctg atattattag tgaattgcca ttaatataat tccgtaacgt ttatcatgtt
    13561 atcctaagga ttatccgaaa aataatacct acgaacatct tccaggatac tcctgcggcg
    13621 aaatatttgt tttaagctca ctcacatatc gcaacattta ctttaagaca attccaggca
    13681 aattatacaa cactttacgg gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt
    13741 aggtgaccca tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt
    13801 tacgcattga gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc
    13861 gactaggttt aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca
    13921 ttgaaaccgt tgatgacctt gccatagctt gtgattcaca gcgcccttca gtggtgttta
    13981 ttaatgagga ctgtttcatc catgatgctt ctaacagtca gcacatcaag cacatcatta
    14041 atcaacatcc caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg
    14101 aatatctatt ggtcagaaaa aaattattga taagttctaa atcgattaaa ccggaatctc
    14161 tcgacgatat ccttggcgat attctgaaaa aagagacaac gataacctct tttttaaata
    14221 tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc
    14281 agggaaccat tcaaatctct gaccaaatga atatcaaagc caagaccgtt tcatcgcata
    14341 aaggtaatat taaacgtaag atcaaaacac ataataaaca ggttatctac catgtcgtcc
    14401 gactgacgga taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg
    14461 gtggtttccc accagtcagg ctgaataaga ttactctgct ttctccacaa agataccgtc
    14521 ctgatgccct gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgaaaccag
    14581 gtacattgtg ccttcactaa actcctcaac tgccagtacc acgccaggac gacgcggacc
    14641 gccatccgtt ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg
    14701 tgccagtgta gaacaatttc gtttttctgg cagctccagg cgcgcgcgag tgctgatttt
    14761 ctcgacggtc tatacttaag agatgccagg cggacttaac gactggcggc aacaacagag
    14821 taacggttgc gaggaaagat gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt
    14881 gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa
    14941 gttcatcgca gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca
    15001 tggcgtgaat tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg
    15061 aatcgttgaa tgcacaataa aaaaatcccg accctgaggg ggtcgggatg aaacttgctt
    15121 aagcaagaag cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat
    15181 ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg aataattaac gctacttatg
    15241 aatagaaatg tgacgtgcgt caccccttgg tggctctggc gtcaacggga tcgctcccga
    15301 aaaaatcatc tttgattatt tgtgctgttt tgagaatcat cctggtaagg ggagaactgc
    15361 acgaccatgt tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg
    15421 ctggacagtc atagttatga ctggcaaccg actgccctct ggctcagccg tttacgcgaa
    15481 gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag cggaaatgct gtacttgcaa
    15541 aaaacgttgg ggctacaagg gttaccactg attgccgaaa atggcgcagt gatccagctt
    15601 gctgaacaat ggcaggatat agacggtttt ccacgcatca tctcaggtat tagccatggc
    15661 gaaatcagcc aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttc
    15721 gatgatgtcg acgatgcaac catcgccgaa tggacgggat taagccgtag ccaggcggcg
    15781 ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg
    15841 gcacaattta ccgctcgtct gaacgaactg ggcttacagt ttatgcaggg tgcgcgcttc
    15901 tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg ccaactggat tatcgcgacc
    15961 tatcaacaat tatcaggcaa acgcccaacc acacttggcc tgggcgatgg gccaaacgat
    16021 gcgcccttac tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg
    16081 gtgcatctgc atgatgagga tccgacccgc gtctggcgaa cgcagcgtga aggaccggaa
    16141 ggctggcgtg aagggctgga ccattttttc tccgctcgtt aagcgttatc gctcgcgcat
    16201 acccgattac gcccggcctg tttagcgaga taaagccgac ggtcagccag tgactggagt
    16261 tgttcaaaat cataatcacc ggtttcctcg ctgctactta cccccaacga ggcactgatg
    16321 cgtatcgtcg tactcttagc aatcaacatc tctttttcat ttaacttcag gcgaatacgt
    16381 tctgcgactt ccgcagcctc cgtcagactc gcgcctggca gaatcacaca aaactcctca
    16441 ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa tccggcagca
    16501 tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg
    16561 tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg tctgacacaa tttcgcgagc
    16621 ggacgggctt tttcaaacag tgcgccacga ttatataaac gggttaaggt gtcgtgccac
    16681 gcctgccact gcaacgagct ttgcagaaca tacatgttgc taaccatccg gcgaatcaca
    16741 taccaggaga tgagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg
    16801 atactgccga aatcgccgcg cacgccttcg cttagcgtat ggacgcgcac cagcacaccg
    16861 tcgaaatggt ccagacgttc ccagctaaca tagcgactgt ccatacgaat gccgccccgc
    16921 gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac gaggatcaaa aatattccct
    16981 gttggatggt caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac
    17041 tcaccatcga ggtttttatc gatggcgttt cttaaaaatt gctgcatggt acgcacggga
    17101 atactcatcc ccagcacgcc ataccagtag ttattactgt ctaccggaac actgacggta
    17161 acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag taaaccagcg taccgcacgg
    17221 tgacgatttt ctcgttgcga atggccgata aaccagggtt gggtgacata gccgtaataa
    17281 cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccggcacgc
    17341 gagacataca tcgcctgttc aaccatcgac gaggtgttgt gcgccagtcg cagcaagtaa
    17401 ccaacttcca gtgcagcggt aatttcattg tcgaggcttt cattttcgcg agacaggaga
    17461 ttcccctcgc tgactaatgc atccgatacg ccattgactg acagggtgcg tcgtcggttg
    17521 agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga actcggttac agcattacgc
    17581 agtgaagtga aatccagtgg cgctacgaga gcttcgcgca tgccattacg caaaaagatc
    17641 agtttgtcga cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga
    17701 ttacgctggc tggagatata ggcatcttcc agcactacca cttcacgcca ggtcagcaag
    17761 gtggaaaaaa gcaataccac gataaagcag agattaacga catgaccagg ccccaggcgg
    17821 cgtgcgagtt ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg
    17881 actcccaacc tgctttttta tgattctggc ataacctggc gatagcgtac cgcaaaacag
    17941 cgataatttt ataatggtgg catgataaga cgcgactatc attcattgca ggcattggca
    18001 caccaggccg gaagtgatcc ggcctggtgt taatgatacc ctttcaaact tcttccgcag
    18061 aacgactccc ctcccccgga acaagctcgg tttcatgaaa agcttcccgc ttgaccccgt
    18121 aaccgtcata ccaacgacac tcaaccatac cgctggagta tccagtgaca atcatccgcg
    18181 ggccgccctc tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt
    18241 ttcgagtgaa acttgttcac cttagttgaa gatggcgaat tttgcataat agcctgtacg
    18301 agtattaatg tgcctgaccg cgcattttcg ctacggcttt cactcccagc accacgatgc
    18361 cgccgatgat aaatccaaga atcagattta aaacagtcgg taatatcatc gccaccactg
    18421 cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag cggcgcaata ccatgtacca
    18481 caataccgcc gccgacgagg aacatcgcca gcgtgccgac aatcgataac gccttcatca
    18541 accagggcgc aatgatcaat aaccctttac ctaatgcctg catcagcgcg ctggattttt
    18601 ccgccagcca ataccccagg tcatcaatct taacgataac ccctaccaga ccgtagacgc
    18661 ccacggtcac taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgctt
    18721 cggccacaat ccccagcgtg atggcgacga tttcggcaga caatataaaa tcggtacgaa
    18781 tcgccccttt tattttgtcc ttttcaaact tcagcggatc ctgcgccgcc agcttctcca
    18841 gacgctgctg gctctgcgcc ggatcttctt tatgtttacg cgcctccagc atatgcagca
    18901 ctttctctac tccttcaaag cagagaaacg cgccaccaat catcaacaga ggcgtaatcg
    18961 cccacgggat aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattaatca
    19021 gcgatccttt cgccacgccc cagaccacgg gaagttcccg gttggcccgc acgcctgaaa
    19081 cttgttgcgc attgagcgat aagtcatccc ctaatacacc ggcggttttc ttcgccgcca
    19141 gtttgcccat cacggagata tcgtccagta gtgtggcgat atcatcgagc aacgtcagta
    19201 aactactccc ggccaaaagc attccctctc atttttattg ttgaagggtg taagtatgaa
    19261 gcaaaaacgg cagctctgat acttgcttca caggtcaaca ttttttttac aatcaagtgc
    19321 aattatatct ctcgccagca gaatgatttt cacgtttact atgaaccacc tttttatttc
    19381 caccgtgagg gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt
    19441 tgtatatcat ttggggctca acctattttg tcattcggat tggcgtggaa agctggcctc
    19501 cgttaatgat ggcaggcgtt cgattcctgg cagccggtat tttattgatg gcatttttgc
    19561 tactgcgcgg acacaagctc cccccgctac gtccgctgct caatgccgcg ctgattggcc
    19621 tgttattgct ggctgtcggt aatggcatgg tgacggttgc cgagcatcaa aatgttcctt
    19681 ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt taccctggtg cttcagccgc
    19741 ctgtttggca ttaaaacgcg caaactggaa tgggtgggta tcgccattgg gcttgccgga
    19801 atcatcatgc tcaatagcgg tggaaattta agcggcaatc cgtggggcgc gattctgatt
    19861 ttaatcggct cgattagctg ggcgtttggc tcggtttatg gctcgcgcat taccttacct
    19921 gtagggatga tggcgggtgc gattgagatg ctggcggcag gcgtggtgtt aatgatcgcg
    19981 tcgatgattg caggtgaaaa actgacggcg ctcccttccc tttcaggctt ccttgcggtc
    20041 ggctatctgg cgctgtttgg ttcgattatc gccatcaacg cttatatgta tttaatccgt
    20101 aatgtcagtc cggctctcgc caccagctac gcctacgtta acccggtggt cgcggtcttg
    20161 ctgggtacgg gactgggcgg agagacgctg tcgaagattg aatggctggc gctcggcgta
    20221 attgtcttcg cggtggtact ggtcacgttg ggaaaatatc tcttcccggc aaaacccgta
    20281 gttgcgccag ttattcagga cgcatcaagc gagtaaatga atcccctgcg tgtcgatctg
    20341 cgcgctggca ccttcgccgc agatccactc ttccagacgc tcggtaagcg cttcatccgt
    20401 cagcttctcg cgcccacgta atgcacactc ccagacaatc agtacgcgcc agccgagttc
    20461 ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt ttacctatct tctccagcca
    20521 gaactcggtt cgagtcgcag gcactttaaa cagatagcag tgatgatgat gccagaagca
    20581 gccatgggta aatatcacgc agcgatattc atcaacgaca aaatccgggc ttccgggcag
    20641 actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt aacagactgg cgaggcgctt
    20701 ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt ttgctgcgag tggccttatc
    20761 gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc caccgcctgt ttgatttttg
    20821 gctcaagcag ttttgccacc gcggcaaaga ccggcacgac caccgagtta ccgaactggc
    20881 gataggcctg agtgtccgaa accggaatgc ggaatttcgc ttctcccggc gcttcaaaac
    20941 ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg acgatgttgc tgattcagcg
    21001 gatcgtcaaa atctttctca cccgtggcca tatcccagcc gcgatcgatt aaaatttccg
    21061 cgccatcttt gtaataacgc gcagacagcg tacgcgtgac gctttgcgga ttgttcggat
    21121 aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg ttttttcgca tatcgataga
    21181 ggtacttcca cagcaccggc gtcaggatat atttcgcctc gaccatcggg tccaacagct
    21241 gcgccagcgt cactcgctgt gcagggaaac attcgctgat atcacgcaga gtaaaatcgg
    21301 ctttcagatt aagatcgcga cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca
    21361 gaaaatgttt accatcgatg attttcggat cgtccggccc gttatcttct gcatcagcca
    21421 cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg gaacgtttta ccctggtcgt
    21481 gacttttcag gtttttgacg ttttcgagca caaacatcgc cggacgacgc gcgtcgataa
    21541 tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc
    21601 cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg ctgacaaggg aaaccggcca
    21661 gtaaaacatc gtgttcagga atgtgttgac gaatatgttc cgccgccgcc tcatcactca
    21721 cgccttcttt atggctgagg gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg
    21781 gatcgcaata atggttggct ttataagtgc gtaccgcatg tttgttccat tcgctggtaa
    21841 acacgcattg tccgccaatc gattcaaaac cgcgacggat gccgccaatt ccggcaaata
    21901 gatcgataaa gcgaaacgca taatgcggat gatgtgccga tggtttgggt aacaacgttt
    21961 gcagatgggc gaactctttc tcacttaaac ggtgccatgc cgagtcattc gccagcgcac
    22021 gttttaaaat tgccgcgctc cagtgattct cacccacacc attaagctgc gccaccagcg
    22081 ttttaacatc ataaatttgc agcagtttct ccagcattgc ctgtgcggca ttcccggtgc
    22141 tgtatgaatc ggttactgat atattttcct gcatagattt aaccggccat ctgagatggc
    22201 cgacaggtta acataattac agccatttag ggccacgcgc ttatcccgcg ggtgaaaacg
    22261 catctatcac cttgtgatcg acaccttcat tctcccccgc cagttcggca ctgagcttcg
    22321 ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag ctgcttgcct cttgcggttt
    22381 gcatggtttg cggtagcttc agcaacttag tctggaaatg atccagcgcg tagcgtttat
    22441 catcaagcgg gcgatgctgt gcgaacggat cttcaccatc aaacagcgcc acgcccaatg
    22501 cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg
    22561 catcctggac gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag
    22621 cggcaatggc atgacaaacg gcctcgattt tctccgccgg aaattgctcg aactcttcac
    22681 ggagcaggcg acgcgtctct tctgccgcca ggattgaaga acgctgcctt tgcggatgat
    22741 ttttcgccag gctcacaata tcgtgaaaat aacaggcggt taaaatcacc agcatatcga
    22801 catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg ctgatgatgg tttttcaacc
    22861 agttttcgaa ctgtgcctgc cagtgttgta agtccatacg cgctccgttg ccaaagagtc
    22921 gccaccttag cagtttttat tcatagcgtc tgctacgtgc gggacgaggt cgggtgtacc
    22981 aggcaatgct gcctaacaat gccccgacca gagccataaa taaaaatccg accagtgccc
    23041 ctaaccactt tcccgcggta gagcctaaat cgctttcaaa tcccgacacc ggggcatcag
    23101 ggatccggct catcacccag atgtaacgcg ccattgccca gacgaaatag ccacaccatg
    23161 tatagaaagc ccacaaagcc aatttgccgc cagggctgcg agagagtctc tcttccatct
    23221 ttgtcgaacc gtattccatt ttgagataac ccggaaaatt cagacttagt actattatgt
    23281 gataaaagtc acatttttcc acattggatg agccagtcaa gactcaatca ttttttgaac
    23341 aaatagataa attttgcgca acggcaataa ttactggaaa aacagcaata aagatttatt
    23401 tatatccttg gtaatagttt tattaattca cccaaacaat atattagcac tgaatacaat
    23461 tatacctttt ttgatttctg tgtcatgata accccgttat tagcctttta tcgtcttgtt
    23521 tatatttttt gggccggcat gatgccggct ttttttatac cttcattaat gtgcgcgtga
    23581 tcacgcctgc cgtttggcgc aacaatcatt gatactccct atctttccat tcaactcata
    23641 acatattgat atacatttat aattttcatg gatgtttata tttagaattc ataattctga
    23701 attatatcaa atagataaga aacattttga aatcttttaa atacatttgt tacacgcaat
    23761 ctttaaa
//
LOCUS       DAZOXR010000054        23764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZOXR010000054 DAZOXR010000000
VERSION     DAZOXR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.054"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..3878)
                     /gene="entF"
                     /locus_tag="ACHUNN_001827"
     CDS             complement(<1..3878)
                     /gene="entF"
                     /locus_tag="ACHUNN_001827"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0576220.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLN"
     gene            complement(3875..4093)
                     /gene="ybdZ"
                     /locus_tag="ACHUNN_001828"
     CDS             complement(3875..4093)
                     /gene="ybdZ"
                     /locus_tag="ACHUNN_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0576221.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4096..5298)
                     /gene="fes"
                     /locus_tag="ACHUNN_001829"
     CDS             complement(4096..5298)
                     /gene="fes"
                     /locus_tag="ACHUNN_001829"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0576222.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5541..7781
                     /gene="fepA"
                     /locus_tag="ACHUNN_001830"
     CDS             5541..7781
                     /gene="fepA"
                     /locus_tag="ACHUNN_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0576223.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7956..8576
                     /gene="entD"
                     /locus_tag="ACHUNN_001831"
     CDS             7956..8576
                     /gene="entD"
                     /locus_tag="ACHUNN_001831"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0576224.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8698..8850)
                     /gene="hokE"
                     /locus_tag="ACHUNN_001832"
     CDS             complement(8698..8850)
                     /gene="hokE"
                     /locus_tag="ACHUNN_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0576225.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9201..9353)
                     /gene="hokE"
                     /locus_tag="ACHUNN_001833"
     CDS             complement(9201..9353)
                     /gene="hokE"
                     /locus_tag="ACHUNN_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0576226.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9322..9590
                     /locus_tag="ACHUNN_001834"
                     /pseudo
     CDS             9322..9590
                     /locus_tag="ACHUNN_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9793..10911
                     /locus_tag="ACHUNN_001835"
     CDS             9793..10911
                     /locus_tag="ACHUNN_001835"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0576227.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            10977..11225
                     /locus_tag="ACHUNN_001836"
                     /pseudo
     CDS             10977..11225
                     /locus_tag="ACHUNN_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            11290..11658
                     /locus_tag="ACHUNN_001837"
     CDS             11290..11658
                     /locus_tag="ACHUNN_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0576228.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11752..12405
                     /gene="nfsB"
                     /locus_tag="ACHUNN_001838"
     CDS             11752..12405
                     /gene="nfsB"
                     /locus_tag="ACHUNN_001838"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0576229.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12513..13760
                     /gene="ybdG"
                     /locus_tag="ACHUNN_001839"
     CDS             12513..13760
                     /gene="ybdG"
                     /locus_tag="ACHUNN_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0576230.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13828..15204)
                     /gene="pheP"
                     /locus_tag="ACHUNN_001840"
     CDS             complement(13828..15204)
                     /gene="pheP"
                     /locus_tag="ACHUNN_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0576231.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15306..18449)
                     /gene="cusA"
                     /locus_tag="ACHUNN_001841"
     CDS             complement(15306..18449)
                     /gene="cusA"
                     /locus_tag="ACHUNN_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0576232.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18461..19684)
                     /gene="cusB"
                     /locus_tag="ACHUNN_001842"
     CDS             complement(18461..19684)
                     /gene="cusB"
                     /locus_tag="ACHUNN_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR0576233.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19700..20032)
                     /gene="cusF"
                     /locus_tag="ACHUNN_001843"
     CDS             complement(19700..20032)
                     /gene="cusF"
                     /locus_tag="ACHUNN_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0576234.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20056..21438)
                     /gene="cusC"
                     /locus_tag="ACHUNN_001844"
     CDS             complement(20056..21438)
                     /gene="cusC"
                     /locus_tag="ACHUNN_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0576235.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21595..22278
                     /gene="cusR"
                     /locus_tag="ACHUNN_001845"
     CDS             21595..22278
                     /gene="cusR"
                     /locus_tag="ACHUNN_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0576236.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22268..23716
                     /gene="cusS"
                     /locus_tag="ACHUNN_001846"
     CDS             22268..23716
                     /gene="cusS"
                     /locus_tag="ACHUNN_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0576237.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVSKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5732 a         6489 c         6033 g         5510 t
ORIGIN      
        1 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
       61 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
      121 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
      181 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
      241 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
      301 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
      361 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
      421 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
      481 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
      541 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
      601 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
      661 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
      721 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
      781 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
      841 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
      901 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
      961 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
     1021 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
     1081 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
     1141 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
     1201 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
     1261 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
     1321 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
     1381 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
     1441 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
     1501 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
     1561 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
     1621 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
     1681 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
     1741 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
     1801 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
     1861 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
     1921 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
     1981 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
     2041 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
     2101 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
     2161 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
     2221 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
     2281 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
     2341 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
     2401 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
     2461 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
     2521 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
     2581 atatcgacat cgccgcacaa cagcgccgga tccgcggcaa actgggcaat cagcattttc
     2641 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
     2701 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
     2761 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
     2821 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
     2881 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
     2941 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
     3001 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
     3061 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
     3121 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
     3181 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
     3241 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
     3301 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
     3361 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
     3421 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
     3481 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
     3541 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
     3601 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
     3661 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
     3721 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
     3781 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
     3841 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
     3901 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
     3961 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
     4021 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
     4081 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
     4141 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
     4201 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
     4261 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
     4321 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
     4381 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
     4441 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
     4501 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
     4561 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
     4621 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
     4681 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
     4741 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
     4801 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
     4861 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
     4921 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
     4981 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
     5041 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
     5101 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
     5161 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
     5221 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
     5281 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
     5341 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
     5401 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
     5461 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
     5521 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
     5581 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
     5641 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
     5701 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
     5761 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
     5821 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
     5881 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
     5941 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
     6001 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
     6061 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
     6121 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
     6181 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
     6241 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
     6301 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
     6361 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
     6421 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
     6481 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
     6541 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
     6601 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
     6661 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
     6721 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
     6781 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
     6841 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
     6901 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
     6961 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
     7021 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
     7081 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
     7141 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
     7201 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
     7261 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
     7321 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
     7381 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
     7441 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
     7501 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
     7561 gtccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
     7621 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
     7681 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
     7741 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
     7801 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
     7861 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
     7921 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
     7981 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
     8041 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
     8101 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
     8161 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
     8221 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
     8281 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
     8341 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
     8401 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
     8461 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
     8521 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
     8581 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
     8641 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
     8701 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
     8761 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
     8821 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
     8881 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
     8941 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
     9001 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
     9061 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
     9121 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
     9181 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
     9241 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
     9301 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
     9361 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
     9421 agggttgata gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa
     9481 ccggaaagct tcaggcaccc gccgttatct tacccttcct caacaaaatg gctgtttatt
     9541 tatacagtaa atcctgtttc ggacttctca ctcctcattt aatcaactga agctgagccc
     9601 gttttttctg catgcctcaa tcgtcaaaat tcttcgccat cagccagctc tgccataact
     9661 tgccacattg aaaaagtagc caaattgctt cagtcaccat catcaaaaaa agaaacattc
     9721 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgccaaca
     9781 taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
     9841 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
     9901 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
     9961 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
    10021 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
    10081 ggtggcacac acccgtttca gaaatggcag cgtcaggagg tatgcgataa cgagcgctat
    10141 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
    10201 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
    10261 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
    10321 cgttttgcct cctcacgacc gaatattttt tccgcctttc ccgataatgg cccgatgccg
    10381 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtttgagtta caccacgatg
    10441 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
    10501 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    10561 attcaggcta ccgcccactg gttactgaca gaacgcccgt tcaaacatca ggagaaagat
    10621 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    10681 accgatccgc acactggcga tcgtcgaccg ctaacggaag acaccttgcg attgctggaa
    10741 aaaatcgccc cttccgcaca taaaattggt gcatcgagcg cgattgaggc cctgcatcgc
    10801 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggtggc
    10861 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccgatgacta agtagggaat
    10921 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    10981 aacacccttt agaaaccttg actaccgcag caggcatttt gctgatggct ttcctctctt
    11041 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
    11101 tccatctgat ggatcttagc tagctttaca ctttattgtt ttgtctgtgg tttttagtgc
    11161 tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
    11221 attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc ttcgcctaaa
    11281 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc ccttgagtta
    11341 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    11401 aaaattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    11461 ccacaaaaat ccctgttaaa tcagcagata tacccgagca ttaagccagg gtatcacatg
    11521 aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    11581 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    11641 agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta ccatttctcg
    11701 ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    11761 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagtaa aaaacttacc
    11821 ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag caccaactcc
    11881 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    11941 gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg
    12001 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    12061 gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    12121 ttcgctgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    12181 tatctcaacg tcggtaactt cctgctcggc gtggcggctc tgggtctgga cgcggtaccc
    12241 atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga gaaaggctac
    12301 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
    12361 ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc tcttgccggg
    12421 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    12481 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    12541 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
    12601 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
    12661 aacgagccat cgccagttca cggctttggt tgcaaatcat aacccagaat aaactcttcc
    12721 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
    12781 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    12841 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
    12901 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcga
    12961 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
    13021 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
    13081 tggtggccgg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    13141 tgccgaaata cggcgcagat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
    13201 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    13261 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
    13321 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    13381 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    13441 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    13501 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
    13561 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
    13621 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    13681 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    13741 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
    13801 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
    13861 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
    13921 catcagcagc aaaatcatgc cgaggaaggc aatgcagaga tagtttccga acggatagag
    13981 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg
    14041 cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    14101 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    14161 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
    14221 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    14281 atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta ccacgttgct
    14341 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
    14401 atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc gatacaccac
    14461 ctgatttacc gcttttggaa tgcttttatc cggatcgcgc gcttcagcgg cagtaatccc
    14521 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
    14581 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
    14641 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga ttgccagcac
    14701 tttaatcaac gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    14761 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    14821 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    14881 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    14941 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
    15001 gaaagcgatg atcccggcga cgccgtaacc cagcaataca gccggacccg ccatctgaat
    15061 cgccggacca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    15121 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    15181 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    15241 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    15301 tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    15361 ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc ggcaatccgg
    15421 ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc ggcgataatc
    15481 accgccaccg tcatcgcttt cgggcgcacg cgcagtaccg cgccgtgata caacgcctca
    15541 tccagcttct gctcactgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    15601 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    15661 aaaccggtgc ccgttgccac agaaagatga aagcccatcc accacagcaa ccagatgccg
    15721 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac ccgacggaat
    15781 gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag tttcagctta
    15841 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt gcccggtttt
    15901 aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac catgtcacga
    15961 tcgcgggcat caatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    16021 ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg cttcatcggc
    16081 gtcaggatcg gtagctggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    16141 agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    16201 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    16261 cggttaatct caacgttgat atagcgccca ccttccagac gctcggctag agcagaagcc
    16321 acgcttggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc gatatccgcc
    16381 agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga gagcatatcg
    16441 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg cacggtgtta
    16501 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    16561 agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt ttccgctttc
    16621 ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt gtcggttttt
    16681 tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg catatacaac
    16741 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    16801 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    16861 agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact gctttccggc
    16921 ggaattttgc cacggatcca gtaccccatc aggataggga tcactacgat cgccagcagc
    16981 gccgcgcccg ccatcgcata ggttttagtg aacgccaacg ggccaaacag acgcccttcc
    17041 tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag actgataaat
    17101 agcgccggac ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    17161 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgtgc attttcgatc
    17221 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccgcc caacgacata
    17281 atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa ccccagcggc
    17341 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    17401 gccaccacga taaactcttc cagcaacttg ccgctgaggt tgtcgatggc gcggtcaatg
    17461 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact acttttcagc
    17521 gtttccagtt tgtccttcac ggaggcgatc acttctcggg cgtttttgcc ggagcgcagg
    17581 atcaccaccc cgcccgccac ttcgccttcg ccattcagtt cggcaatgcc ccggcgcatc
    17641 tccgggccaa tctggacctt cgcaacatca cgcagataaa cgggcacgcc attttctctg
    17701 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    17761 atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc
    17821 agcgcgcttt ttacttcggc tagactgatg ccatattgcg ccaggcgctg gggatcgata
    17881 accacctgat actctttcac cacgccgccc accgacgcca cttccgcaac gtcagggatg
    17941 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    18001 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggt
    18061 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    18121 acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata cacataagag
    18181 tctccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    18241 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    18301 ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt gttaatgatg
    18361 gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc
    18421 accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca ttggtagcac
    18481 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca atcaggaaca
    18541 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gtgtaacgcg gtgacgcctt
    18601 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg tccgtcggca tcaacggtaa
    18661 tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    18721 cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    18781 tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca ggtagcagcg
    18841 tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagc gtaaactgcg
    18901 aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc cacaccgggt
    18961 ccataccctg aattttcgct accacgttat ctttggcgat attcattccc gcgcgcagat
    19021 caaacgcggt gatcacgcca tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt
    19081 gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc agccgctcaa
    19141 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    19201 gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt tgtactttat
    19261 cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc tgcacaatgg
    19321 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc
    19381 cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    19441 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    19501 gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac cagaataaga
    19561 ttttacgttc tgcggtcgac gttttttctg cgggtggttc agcctttgca aaccaggtaa
    19621 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    19681 tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa gaaaggttgc
    19741 cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc attttcgtct
    19801 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    19861 gatggatggt gatttttttg ctttccagat caataccctt taccacgcca gtggcgctaa
    19921 taacctgcgg ttgtacttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    19981 agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc atttattctc
    20041 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtataca aagaaatttc
    20101 gttaacctga cgggcataat tcaggtcgag taaagtttgt cgggttgcaa ataaagaacg
    20161 ctcggcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg cccgcgcccg
    20221 ttgcaaagta atttgcagcg acgccagata acgctgctgg gcgctgattt gatcgttcag
    20281 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga ttttctgttc
    20341 ataattcact accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg
    20401 tccggcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctagc
    20461 gttaaataat gaagacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    20521 cgggaaaaat gccgcacgcg cagcaccaat attagcatta gccgccatta acgcgtgttc
    20581 agcttccata atatccgggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt
    20641 aacgctttgc aggctgccgc tgtttactgt ctgcgcttgc ggcagtttgc cgtagcttcc
    20701 caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc
    20761 actgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    20821 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    20881 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    20941 aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc
    21001 ggcttcgctc atgttcttta agcgaccgaa aaaatcgagg tcaaagctgg cgttaaggcc
    21061 ggtcgaaaac tcccgtgtcg tggcggtatt ccctttaaga ttaccgctcc agctgccgct
    21121 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc
    21181 ctcctgcact ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat
    21241 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccacgttct ggtagttatc
    21301 tgctgcgtta accaagccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgt
    21361 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    21421 aagtttacaa ggagacatag gctcataatt tctggtgatt ttataccgcc aactttactc
    21481 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    21541 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaaaatgaaa
    21601 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    21661 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    21721 ggtgattatg acctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    21781 gtgcgcatgt tgcgctccgc caataaaggg atgccgattc tgttgcttac cgcgcttggc
    21841 accattgaac atcgcgtcaa ggggctggag ttgggggccg atgactatct ggtgaagccg
    21901 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg
    21961 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    22021 acccgcagcg gcacgcgcat cactctgacc agtaaagagt ttactctgct ggagtttttt
    22081 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt gtgggacatg
    22141 aatttcgaca gcgacactaa cgccattgat gtggcggtga agcggctacg cggcaaaatc
    22201 gacaacgact ttgagccgaa gctgattcag accgtgcgcg gcgtgggcta tatgcttgag
    22261 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gtctgacctt
    22321 ttttatcagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    22381 agtgaaagtg cattttgccg agcaggatat taatgattta aaagaaatta gcgccaccct
    22441 tgaacgggta cttaatcatc ctgacgagac gcaagcccga cgcttaatga cgctggaaga
    22501 tatcgtcagt ggttattcca acgtgttgat ttccctggca gatagccacg gcaaaacggt
    22561 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    22621 agacgctcag ggtggcgagg tgtatctcct ttccggcccg acgattatga tgccaggcca
    22681 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgtt
    22741 ggtggacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    22801 ttacataaat gatttgatga ataaacttat tatgaccgca tctgtcatca gcatcctgat
    22861 cgtctttatc gtgctattgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    22921 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgagcc
    22981 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    23041 ctttacccgc cagtccaatt tctcagcgga tatcgctcac gaaattcgca cgccgattac
    23101 caatctcatt acgcaaacgg aaatcgccct cagccagtct cgcagccaga aggagctgga
    23161 agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat
    23221 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    23281 ggcggatgaa gtcagcaaag tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt
    23341 ggagttgcga tttgttggcg acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg
    23401 ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccaaccagag agacaattgt
    23461 agtgcgctgt cagacggtcg atcaccaggt gcaagttacc gtcgaaaacc ccggtacgcc
    23521 cattgcgccc gagcacttac cgcgattgtt tgaccgtttc taccgcgttg acccttcccg
    23581 ccagcgaaaa ggcgaaggta gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc
    23641 gcataaaggc acggttgcag taacgtcaga tgcgcggggg acaaggtttg tgattatgct
    23701 gccggagaga gagtgattat taagttcaaa ttcagggcgt ggtgcatcac ctcctggtat
    23761 tgtt
//
LOCUS       DAZOXR010000055        23235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZOXR010000055 DAZOXR010000000
VERSION     DAZOXR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.055"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="ACHUNN_001847"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="ACHUNN_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0576238.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="ACHUNN_001848"
     CDS             507..1136
                     /locus_tag="ACHUNN_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0576239.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="ACHUNN_001849"
     CDS             1163..2824
                     /locus_tag="ACHUNN_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0576240.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="ACHUNN_001850"
     CDS             3063..3122
                     /locus_tag="ACHUNN_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0576241.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="ACHUNN_001851"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="ACHUNN_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR0576242.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="ACHUNN_001852"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="ACHUNN_001852"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0576243.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="ACHUNN_001853"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="ACHUNN_001853"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0576244.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="ACHUNN_001854"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="ACHUNN_001854"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0576245.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="ACHUNN_001855"
     CDS             9505..10125
                     /locus_tag="ACHUNN_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0576246.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="ACHUNN_001856"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="ACHUNN_001856"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0576247.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="ACHUNN_001857"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="ACHUNN_001857"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0576248.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="ACHUNN_001858"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="ACHUNN_001858"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0576249.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="ACHUNN_001859"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="ACHUNN_001859"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0576250.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWSVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="ACHUNN_001860"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="ACHUNN_001860"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="ACHUNN_001861"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="ACHUNN_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR0576251.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="ACHUNN_001862"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="ACHUNN_001862"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0576252.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="ACHUNN_001863"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="ACHUNN_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0576253.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="ACHUNN_001864"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="ACHUNN_001864"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0576254.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="ACHUNN_001865"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="ACHUNN_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0576255.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="ACHUNN_001866"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="ACHUNN_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0576256.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="ACHUNN_001867"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="ACHUNN_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0576257.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6330 c         5907 g         5346 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc gtgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggtccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac ctttt
//
LOCUS       DAZOXR010000056        22703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZOXR010000056 DAZOXR010000000
VERSION     DAZOXR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.056"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUNN_001868"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUNN_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0576258.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUNN_001869"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUNN_001869"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0576259.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUNN_001870"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUNN_001870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0576260.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUNN_001871"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUNN_001871"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0576261.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUNN_001872"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUNN_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0576262.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="ACHUNN_001873"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="ACHUNN_001873"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR0576263.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHUNN_001874"
     CDS             complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHUNN_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0576264.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHUNN_001875"
     CDS             complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHUNN_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0576265.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="ACHUNN_001876"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="ACHUNN_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0576266.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="ACHUNN_001877"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="ACHUNN_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0576267.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="ACHUNN_001878"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="ACHUNN_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0576268.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="ACHUNN_001879"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="ACHUNN_001879"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0576269.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="ACHUNN_001880"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="ACHUNN_001880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0576270.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="ACHUNN_001881"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="ACHUNN_001881"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0576271.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="ACHUNN_001882"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="ACHUNN_001882"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0576272.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHUNN_001883"
     CDS             14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHUNN_001883"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0576273.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHUNN_001884"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHUNN_001884"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0576274.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHUNN_001885"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHUNN_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0576275.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHUNN_001886"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHUNN_001886"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0576276.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHUNN_001887"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHUNN_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0576277.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHUNN_001888"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHUNN_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0576278.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHUNN_001889"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHUNN_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0576279.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHUNN_001890"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHUNN_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0576280.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHUNN_001891"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHUNN_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0576281.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..22596
                     /locus_tag="ACHUNN_001892"
                     /pseudo
     CDS             22225..22596
                     /locus_tag="ACHUNN_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         5089 a         5689 c         6450 g         5475 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaat atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtttg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcatcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccata gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat ggcgctagac tggccccctg aatctccaga
    22621 caaccagtat cacttattta agtgatagtc ttaatactag tttttagact agtcattgga
    22681 gagcagatga ttgatgtctt agg
//
LOCUS       DAZOXR010000057        22393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZOXR010000057 DAZOXR010000000
VERSION     DAZOXR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.057"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUNN_001893"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUNN_001893"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0576282.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUNN_001894"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUNN_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0576283.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUNN_001895"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUNN_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUNN_001896"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUNN_001896"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0576284.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUNN_001897"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUNN_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0576285.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUNN_001898"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUNN_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0576286.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUNN_001899"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUNN_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0576287.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUNN_001900"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUNN_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0576288.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUNN_001901"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUNN_001901"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0576289.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUNN_001902"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUNN_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0576290.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUNN_001903"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUNN_001903"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0576291.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUNN_001904"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUNN_001904"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0576292.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHUNN_001905"
     CDS             complement(17699..18322)
                     /locus_tag="ACHUNN_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0576293.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUNN_001906"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUNN_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR0576294.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHUNN_001907"
     CDS             complement(18971..19213)
                     /locus_tag="ACHUNN_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0576295.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUNN_001908"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUNN_001908"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0576296.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHUNN_001909"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHUNN_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0576297.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHUNN_001910"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHUNN_001910"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0576298.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         6319 a         5226 c         4871 g         5977 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattt tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatccatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca aga
//
LOCUS       DAZOXR010000058        22311 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZOXR010000058 DAZOXR010000000
VERSION     DAZOXR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.058"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(110..3259)
                     /gene="acrB"
                     /locus_tag="ACHUNN_001911"
     CDS             complement(110..3259)
                     /gene="acrB"
                     /locus_tag="ACHUNN_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0576299.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3282..4475)
                     /gene="acrA"
                     /locus_tag="ACHUNN_001912"
     CDS             complement(3282..4475)
                     /gene="acrA"
                     /locus_tag="ACHUNN_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0576300.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4617..5264
                     /gene="acrR"
                     /locus_tag="ACHUNN_001913"
     CDS             4617..5264
                     /gene="acrR"
                     /locus_tag="ACHUNN_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0576301.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5392..8754
                     /gene="mscK"
                     /locus_tag="ACHUNN_001914"
     CDS             5392..8754
                     /gene="mscK"
                     /locus_tag="ACHUNN_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0576302.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8815..8913
                     /locus_tag="ACHUNN_001915"
                     /pseudo
     CDS             8815..8913
                     /locus_tag="ACHUNN_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8966..9127)
                     /gene="rsmS"
                     /locus_tag="ACHUNN_001916"
     CDS             complement(8966..9127)
                     /gene="rsmS"
                     /locus_tag="ACHUNN_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0576303.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(9141..9668)
                     /gene="priC"
                     /locus_tag="ACHUNN_001917"
     CDS             complement(9141..9668)
                     /gene="priC"
                     /locus_tag="ACHUNN_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0576304.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9738..10115
                     /locus_tag="ACHUNN_001918"
     CDS             9738..10115
                     /locus_tag="ACHUNN_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0576305.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10268..10819
                     /gene="apt"
                     /locus_tag="ACHUNN_001919"
     CDS             10268..10819
                     /gene="apt"
                     /locus_tag="ACHUNN_001919"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0576306.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10948..12873
                     /gene="dnaX"
                     /locus_tag="ACHUNN_001920"
     CDS             10948..12873
                     /gene="dnaX"
                     /locus_tag="ACHUNN_001920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0576307.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12206..12270
                     /gene="dnaX"
                     /locus_tag="ACHUNN_001920"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12926..13255
                     /locus_tag="ACHUNN_001921"
     CDS             12926..13255
                     /locus_tag="ACHUNN_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0576308.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13255..13860
                     /gene="recR"
                     /locus_tag="ACHUNN_001922"
     CDS             13255..13860
                     /gene="recR"
                     /locus_tag="ACHUNN_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0576309.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13970..15844
                     /gene="htpG"
                     /locus_tag="ACHUNN_001923"
     CDS             13970..15844
                     /gene="htpG"
                     /locus_tag="ACHUNN_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0576310.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16025..16669
                     /gene="adk"
                     /locus_tag="ACHUNN_001924"
     CDS             16025..16669
                     /gene="adk"
                     /locus_tag="ACHUNN_001924"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0576311.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16801..17763
                     /gene="hemH"
                     /locus_tag="ACHUNN_001925"
     CDS             16801..17763
                     /gene="hemH"
                     /locus_tag="ACHUNN_001925"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0576312.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17760..18719)
                     /gene="aes"
                     /locus_tag="ACHUNN_001926"
     CDS             complement(17760..18719)
                     /gene="aes"
                     /locus_tag="ACHUNN_001926"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR0576313.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18871..20175
                     /gene="gsk"
                     /locus_tag="ACHUNN_001927"
     CDS             18871..20175
                     /gene="gsk"
                     /locus_tag="ACHUNN_001927"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0576314.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20308..21984)
                     /gene="ybaL"
                     /locus_tag="ACHUNN_001928"
     CDS             complement(20308..21984)
                     /gene="ybaL"
                     /locus_tag="ACHUNN_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0576315.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22222..>22311)
                     /locus_tag="ACHUNN_001929"
     CDS             complement(22222..>22311)
                     /locus_tag="ACHUNN_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576316.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5433 a         5916 c         5853 g         5109 t
ORIGIN      
        1 atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa tgttcgtagg
       61 ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat caatgatgat
      121 cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg cgaaccacca
      181 caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc ccgcccatta
      241 caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata accagcggca
      301 taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc atccgcactg
      361 catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct ttggcgaatt
      421 cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg cctacctgga
      481 agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg ataaccccca
      541 gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc gccgccagac
      601 acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg ccggagagac
      661 gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt ttgctcgcca
      721 gttgttccat cagctccatt gcttcaccgg tacttttacc cggtgccgcc tggcctaaga
      781 tttccatgga tggcaggccg ttgtaacgtt ccagacgcgg cgaaccgtac tcccaacgag
      841 aagaggagaa cgccgagaat ggcaccatct gaccatcagc agcacgaaca taccagtcgc
      901 cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact ttcttcacac
      961 gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc agagaggtgt
     1021 taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata tcaatcttaa
     1081 actgcggggt atcttccaga ccgtttggac gtacgctggt cagcatatca gggtgcttcg
     1141 ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca aggccagcct
     1201 ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt gcgggcaggt
     1261 taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca cgcatggtaa
     1321 tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag gaaacgaacg
     1381 caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg aacaccgact
     1441 caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc actttctgcg
     1501 tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg ccctggtcct
     1561 catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg accacgatga
     1621 tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg ctgtcggtgt
     1681 agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct tttttacctt
     1741 ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc ggagtcagga
     1801 tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag aactgacgat
     1861 agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgcc gacagtacca
     1921 tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta gcttcttttg
     1981 gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg atagcgtcat
     2041 ccaccaacaa gccgatggcg agcaccatcc cgaacattgt tagcgtgttt atcgagaagc
     2101 caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca atggtcggaa
     2161 tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg aggatgatcg
     2221 cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc gtggtgtcgt
     2281 acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc agttcagcac
     2341 ggattgccgc agcggtatcc agcgcgtttg caccggtcgc cagcttgatc cccagaccgg
     2401 aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc agctcaatct
     2461 tcgccacatc acgcagcagt acgcgggaac catcctgatt cactttcagc aggattttgc
     2521 cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta agctgttggc
     2581 ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc tgcgctttga
     2641 tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc gggttcatcc
     2701 agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc gacgtacggc
     2761 tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc gtcatggtgc
     2821 catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc tcaacgctca
     2881 ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt ttgttctgca
     2941 cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg atctgcacgg
     3001 tacccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg ttcatattct
     3061 gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg gggtaggagg
     3121 cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc agtttgagga
     3181 tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa atcgggcgat
     3241 cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt ggactgttca
     3301 ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg tgcttttacc
     3361 tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc tgctttcaga
     3421 ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg acgggtttcc
     3481 actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt acgggttacg
     3541 ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg tgcgcgcacg
     3601 aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag ggtgatagac
     3661 ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg cgggaactta
     3721 atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt cagcgtgcca
     3781 ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac atcaaggtag
     3841 atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg taccaatgcg
     3901 ccttccgtca cgttcgattt accaatgcga ccgctaatcg gagaggtgac tttggtgtaa
     3961 gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc agcattcgcc
     4021 tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg agtaccgagc
     4081 agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg ggctttcgcc
     4141 agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat ctgatagaga
     4201 gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat aatcccgcta
     4261 acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag ctcggttgtg
     4321 atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg ctggccacct
     4381 tgttgggcct gtttgtcgtc acatcctgtt agggctaagc tgcctgagag catcagaacg
     4441 accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt gtccgatttc
     4501 aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta tcgtcgtgct
     4561 atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac gaacatatgg
     4621 cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat gtggctctac
     4681 gtcttttctc acagcagggg gtatcatcca cctcgctggg cgagattgca aaagcagctg
     4741 gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg ttcagtgaga
     4801 tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag gcaaaattcc
     4861 ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa tccacggtga
     4921 cagaagaacg acgtcgatta ttgatggaga ttatattcca caaatgcgaa tttgtcggag
     4981 aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat gaccgtatag
     5041 aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta atgacgcgtc
     5101 gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg ctctttgccc
     5161 cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta ctggagatgt
     5221 atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga atctgactcc
     5281 aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg tgatattctt
     5341 gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat catgactatg
     5401 ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc ttttgttttt
     5461 gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga tctgccgaca
     5521 aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct ttctgctcag
     5581 gacaaactgg tgcagcagga tctgacagat acattagcca ccctcgataa aatcgatcgc
     5641 ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga aaaaatgcgc
     5701 caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga aacgcgcaaa
     5761 attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc gctggacgat
     5821 ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc gttacagacg
     5881 cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca acaaattcgc
     5941 agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca gaaagtgtta
     6001 atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa aagcctggaa
     6061 gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac ggcgaacagc
     6121 gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa gcgcctgact
     6181 ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg tattcaggct
     6241 aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg tctgattacc
     6301 gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa ctggctggag
     6361 cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa gggcagcctg
     6421 ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga tgaactggaa
     6481 aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa ccagcagcgt
     6541 gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca caccaacgaa
     6601 gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg cgaattgctg
     6661 gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct gcaaatcaac
     6721 cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca gcaaatcttt
     6781 tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc gcaaagcctg
     6841 aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg gcctgccgtt
     6901 tttatcgctt tcctcgccgg tttgccgctg ctgttgattg ccgggctgat ccactggcgt
     6961 ctgggctggc tgaaagcgta tcaacagaaa ctggcttccg ctgtgggttc cctgcgtaac
     7021 gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc gctgccggtg
     7081 tgcctgatta ttctcgcggt tggcctgatt ctgttgacca tgcagctcaa catcagcgaa
     7141 ctgctatggt cgttcagtaa aaaactggcg attttctggc tggtgtttgg cctgtgctgg
     7201 aaggtactgg agaaaaatgg cgttgccgtg cgtcacttcg gcatgccgga gcagcagacc
     7261 agccactggc gtcggcaaat tgtccgcatc agtctcgcgt tgctgcctat ccatttctgg
     7321 tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg gcaggcgatg
     7381 attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg cgaaagctgg
     7441 cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc gattatcccg
     7501 attgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg cctctccggg
     7561 cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca gacggtattg
     7621 cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc gcgtcggcag
     7681 aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac cattgcactg
     7741 gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc gctgttcggt
     7801 gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatct cgacagcatc
     7861 acgctctggc attacaacgg cactgaagct ggcgctgcgg tggtgaaaaa cgtcaccatg
     7921 ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat tcgcaacctg
     7981 cctggtttac tggaagtgct ggtgctctcg cgactgaata tgcgtcaggg cgcgtcgtat
     8041 gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac ggtgttcgga
     8101 tcgctgggcg tctcttggga taaactccag tggctggccg cagcattatc cgtaggtctt
     8161 ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat tctgttcgaa
     8221 cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac ggtaagtaag
     8281 atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat catcccgaac
     8341 aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac tacgcgtctg
     8401 gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa agtgttactg
     8461 aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt cttctttacg
     8521 gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga actgcgtgac
     8581 cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg tgaaaacgac
     8641 atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg cgatgaggtg
     8701 acggaagtca aacgcgacta caaaggcgat gacccgacgc cagcggtagg gtaataacca
     8761 aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt tcatgccgga
     8821 tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta ttgcaggagc
     8881 ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac aatcttaggg
     8941 gacatttagc ggccccattt atttctcact tttccgcctt atcatcgcgc gttaatttct
     9001 tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct gggatctgat
     9061 tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg gcattttcaa
     9121 gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc gattttttcc
     9181 agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg atgcagcgtt
     9241 tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgttcggc aaccatctca
     9301 cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg ggcaattttc
     9361 ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc gatggcttcc
     9421 agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg ctgctcaacc
     9481 gcatgacaaa gcgcagccag attatcgccc gcctcgtcga gacaagcctg tagtgttgtc
     9541 gcacgagtct gaaaaagatg cctgtcgaaa cgagcactta gcgtggcgaa ctgtgacacc
     9601 ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag cagcagggcg
     9661 gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag ccactctgta
     9721 agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg ctggcggtag
     9781 tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt atcctgctgg
     9841 cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg taccgctcat
     9901 ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc ggcgtcaaac
     9961 cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc gtccagatgc
    10021 catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat atgtggcgaa
    10081 ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca gttgcaatta
    10141 ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt caaaggttaa
    10201 acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa caagcaggca
    10261 tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca tcaaaagcat
    10321 tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac tggaagaccc
    10381 gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg cgggcattac
    10441 caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag ctctgggtct
    10501 gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa ccatcagtga
    10561 aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg ccatcaaacc
    10621 gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta tcgaagcgac
    10681 cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca ttatcaacct
    10741 gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct acagccttgt
    10801 cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg gacagcacaa
    10861 gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc ttcgtgaatc
    10921 caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg aaaatggcgc
    10981 ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact ggcgaacggc
    11041 ttgtcgttag ggcgtattca tcatgcttat cttttttccg gcacccgtgg cgtcggaaaa
    11101 acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat taccgcgacg
    11161 ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt cgatctgatt
    11221 gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct ggataacgtc
    11281 cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt gcatatgctg
    11341 tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga gcacgttaag
    11401 tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc gcgctgtttg
    11461 caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcaacttga gcacatcctc
    11521 aacgaagaac atatcgctca cgagccgcgg gcgttgcaat tgctggcgcg cgccgctgaa
    11581 ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg tgacggccag
    11641 gtttcaaccc aggcagtcag tgcgatgctg ggtacgcttg atgacgatca ggcgctgtcg
    11701 ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat taatgaagcc
    11761 gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct gttgcatcgt
    11821 attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc cattgagctg
    11881 cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta ctatcagacg
    11941 ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg cgttgagatg
    12001 acgctgctgc gcgcgctggc gttccatccg cgtatgccgc tgcctgagcc agaagtgcca
    12061 cgccagtcct ttgcacccgt cgtgccaacg gcagtaatga cgccaaccca ggtgccgccg
    12121 caatcagcgc cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg
    12181 gcggcgcgcc agcagttgca gtgcgtgcag ggagcaacca aagcaaaaaa gagtgaatcg
    12241 gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc
    12301 accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg aaaaagcgtc agccaaaaaa
    12361 gaagcgtatc gctggaaggc gaccactccg gtgatgcagc aaaaagaagt ggtcgccacg
    12421 ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagcta
    12481 gcggcagaag ccattgagcg cgacccgtgg gcggcacagg tgagccaact ttcgctacca
    12541 aaactggtcg aacaggtggc gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt
    12601 ctgcatttgc gctcctctca gcggcatttg aacaaccgcg gtgcacagca aaaactggct
    12661 gaagcgttga gcatgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat
    12721 cccgcggtgc gtacgccgct ggagtggcgt caggcgatat acgaagaaaa acttgcgcag
    12781 gcgcgcgagt ccattattgc ggataataat attcagaccc tgcgtcggtt tttcgatgcg
    12841 gagctggatg aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc
    12901 cttaacgtga ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa
    12961 gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga
    13021 agtcaccggc gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg
    13081 ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct
    13141 ggtggctgca gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat
    13201 ggcctctgta tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa
    13261 accagcccgc tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg
    13321 aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt
    13381 ttggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcact
    13441 ttcaccgaac aggaagtctg taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa
    13501 atctgtgtgg tggagagtcc ggcggacatc tacgccattg agcagacggg gcagttttct
    13561 ggtcgttatt ttgtgttgat ggggcatctg tcaccgctgg acggcatcgg tccggatgat
    13621 atcgggcttg atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc
    13681 gccaccaatc ccacggttga aggtgaagct accgctaact acattgccga gctttgcgcg
    13741 caatatgacg tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa
    13801 atggtcgatg gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa
    13861 gcaaacgaga gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct
    13921 ccctcatcct gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca
    13981 agaaactcgt ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct
    14041 ctattccaat aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga
    14101 caagctgcgt ttccgtgcgc tatctaaccc ggacctgtac gaaggtgatg gcgaactgcg
    14161 cgttcgtgtc tctttcgata aagacaagcg cacgctgacc atctctgata acggcgtggg
    14221 gatgacccgc gacgaagtga ttgaccatct ggggactatc gctaaatccg gtaccaaatc
    14281 attcctcgaa tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg
    14341 tgttggtttc tactctgcgt ttatcgtggc cgacaaagtg accgtgcgta ctcgcgcggc
    14401 aggcgaaaaa ccagaaaacg gcgtcttctg ggaatcggct ggcgaaggtg aatataccgt
    14461 tgccgacatc accaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga
    14521 agacgagttc ctcgatgact ggcgcgtgcg ttccatcatc agcaaatact ccgaccatat
    14581 cgcgctgccg gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg
    14641 ggagaaaatc aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga
    14701 agagtacaaa gagttctaca aacacatcgc ccacgacttt aatgatccgc tgacctggag
    14761 ccacaaccgc gttgaaggta agcaggagta caccagcctg ctgtacattc cgtcccaggc
    14821 tccgtgggat atgtggaacc gtgatcataa acacggcctg aaactgtacg tccagcgtgt
    14881 gttcatcatg gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct
    14941 gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt
    15001 aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc
    15061 gaaagacgac gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga
    15121 aggtccggcg gaagatttcg ctaaccagga agcgatcgcc aaactgctgc gttttgcttc
    15181 tacccatacc gattcttctg cgcagaccgt atctctggaa gactacgttt cccgcatgaa
    15241 agaagggcag gagaaaatct actacatcac cgcagacagc tatgcggcag cgaagagcag
    15301 cccgcacctg gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat
    15361 cgatgagtgg atgatgaact atctgactga gttcgacggt aaaccgttcc agtcggtgtc
    15421 taaagttgac gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc
    15481 ggagaaagca ctgactccgt tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa
    15541 agatgtccgt ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga
    15601 cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt
    15661 gaaatacatc ttcgaactga acccggatca cgtactggtg aaacgtgcgg cagatactga
    15721 agatgaagcg aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga
    15781 acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc
    15841 ctgatgtcat gccggatgac ctttgtgtca tccggcattt ttcttttcat catctgcact
    15901 ttccgcaaat tatctcgcca ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg
    15961 cctgttggtg gtatcgttta tcgctttttc aaaaaaatcg acacatttta aggggatttt
    16021 cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt
    16081 catcatggag aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt
    16141 caaatctggc tccgagctgg gtaaacaagc aaaagacatt atggatgctg gcaaactggt
    16201 caccgacgaa ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgtaa
    16261 tggtttcctg ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc
    16321 gggcatcaat gttgattacg ttctggaatt cgacgtaccg gacgaactga ttgttgatcg
    16381 tatcgtaggc cgccgcgttc atgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc
    16441 gccgaaagta gaaggcaaag acgacgttac cggtgaagaa ctgactaccc gtaaagacga
    16501 tcaggaagaa accgtgcgta aacgtctggt tgaataccat cagatgactg caccgctgat
    16561 cggctactac ttcaaagaag cggaagcggg taacaccaaa tacgcgaaag ttgacggcac
    16621 caagtcggtt gctgaagttc gcgctgatct ggaaaaaatc ctcggctaat caagccgcag
    16681 cagatctgct ttcgggcagg tctgctcatc cacctcaggc aatacctctc acagcaatta
    16741 gttcttcttc ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta
    16801 atgcgtcaga ctaaaatcgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca
    16861 cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc
    16921 tcacggttgt tatggtggcc attgctgcgc ggcgttattt tgccgctgcg ctcgccgcgt
    16981 gtggcgaagc tgtacgcctc ggtctggatg gaaggtggct cgccgctgat ggtttacagc
    17041 cgccagcaac agcaggcgct ggcacaacgt ttaccggaga cgcccgtagc gctgggaatg
    17101 agctacggct cgccatcact ggaaagcgcc gtcgatgaac tcctggcaga gcatgtagat
    17161 catattgtgg tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg
    17221 gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt
    17281 gattacgccg ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt
    17341 gccaaacatg gcgaaccgga tctgctgctg ctctcttatc atggcattcc ccagcgttat
    17401 gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa cgactcgcga actggcttcc
    17461 gcactgggga tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggccgggaa
    17521 ccctggctga tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt
    17581 catatacagg tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt
    17641 gccgagcaaa accgtgaggt cttcctcggt gcgggcggga aaaaatatga atatattcca
    17701 gcgcttaatg ccacgccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc
    17761 taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc
    17821 atccgtgaat aatgcaaaaa ggcatgcagc gtacctgggt agagtttgaa ctcacagggc
    17881 tgctgatgcg ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac
    17941 tccgccccgg caataaaaca gggcggaact tcgcgagtga gatcattatt aaacagacag
    18001 taatacggtg actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgcaaa
    18061 tcctgttgcg ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt
    18121 aatccgtaaa gcccatacca cagcaaaaca cccgcaattt taccgcaatc gatctgttta
    18181 tcacgcaacc acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag
    18241 ccaatgcggg acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaagca
    18301 gccacaattt cctctatcgc ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaata
    18361 ccaatcaccg tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg
    18421 agattgccga gaataaaacc gcctccatgc aaataaaata gcgtcgccgg gctatctggc
    18481 tgcggacaaa agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg
    18541 gttgccattt ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt
    18601 tgctcagcaa tcgttcccgt tgcgggccag gacggtaaat ccggctgaag agtattcaca
    18661 acggtcttca tttcagcaga aataaggtcc agaacaggta gtttgttttc cggcttcata
    18721 caaaaactcc tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg
    18781 cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg
    18841 ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa
    18901 cattacttcc ctgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa
    18961 accagcgccg cctgggtcgt aggtattgac cagacgctgg tcgatattga agcgaaagtg
    19021 gatgatgaat tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat
    19081 gacgtagccg aagcgcttta tcaggagcta aaacagaaaa acctgattac ccatcagttt
    19141 gcgggtggca ccattggtaa caccatgcac aactactcgg tgctcgcgga cgaccgttcg
    19201 gtgctgctgg gcgtcatgtg cagcaatatt gaaattggca gttatgccta tcgttacctg
    19261 tgtaacactt ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt
    19321 cgttgcttta cgctgattgg cgagtccggg gaacgtacct ttgctatcag tccaggccac
    19381 atgaaccagc tgcgggctga aagcattccg gaagatgtga ttgccggagc ctcggcactg
    19441 gttctcacct cttatctggt gcgttgcaaa ccgggtgaac ccatgccgga agcaaccatg
    19501 aaagccattg agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggcaccaag
    19561 tttgtcattg ccgagaatcc gcagtggtgg cagcaattcc tcaaagatca cgtctctatc
    19621 cttgcgatga acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca
    19681 tctgacaagg cgctggactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg
    19741 tatatggcgg gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg
    19801 ggcgctatag cggaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgc
    19861 cagaatccgc tgcgtgtcta ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc
    19921 atgaacacta atggagcggg ggatggcgca ttggcagcgt tgctgcatga cattaccgcc
    19981 aacagctacc atcgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact
    20041 tattcatcgt tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac
    20101 cagcattcac ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct
    20161 tactgggatc gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct
    20221 ggcatttggc tctcgatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt
    20281 tcatatttta catccggcaa ccaccgttta ccccgtcacc acttcacccg ccggtggcgt
    20341 ttccagcagt tccagcatag tacgggcaat ttcacgttca cccatcacta cctgattcgc
    20401 gccacgttcg gtgatatacg tcacttcatc gtcataatgg gcgcgggcga taatctcaat
    20461 atccgggttt ttcgcgcggg cagacgccac aatctcaccc gcttcataac cgttggggat
    20521 cgtcaggatc agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc
    20581 tgcattgccc aatacagcgc ggaccccacg ctctcgcagc tcatcaacac gggttcgtga
    20641 cgtctcaatc accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc
    20701 tacacgaccg taacccacca atagcgcatg gttgcaaata tccactggga tctgtttctc
    20761 ttcttcgatt gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt
    20821 ctccagtagt gcgaacagta ccgggttgag cataatcgac aggatcgccc ctgccagtac
    20881 caggttttgt ccggcctgcg gcagtaaatt caatgccatt cccagtcccg ccaggataaa
    20941 cgcgaactca ccaatctgcg ccaggctggc ggcgatggtt aatgccgtac gttgggagtg
    21001 accaaacaga cggacaagga acagggccgc gaccgattta ccaaacagaa taattgccag
    21061 cgtcgccagc actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac
    21121 ggagacaaaa aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg
    21181 gtgactcagt tcagactcgt tcagtaccat cccggcaaag aacgcgccaa gtgcgaagga
    21241 gacgtcaaac aactccaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag
    21301 ggtaaatagc tcgcgagaac cggttgccgc gctgcgtgcc ataatccacg gcaccagacg
    21361 gcgacctacc agcatcataa tggcgataaa tgcgatcact ttgccgatgg tgatccccat
    21421 atcgactgca agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg
    21481 cagcaacacc agcgtcagaa ccattaccag gtcttccaca atcaaccaac cgatggcgat
    21541 ttgcccacgc tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt
    21601 actggcggtg gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac
    21661 ggcagagagc gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat
    21721 ggcgatggcc tttaccgcca tcaaatcctt cagcgaaaag tgcaaaccga cgccaaacat
    21781 caacagaatg acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc
    21841 cggagtgaat ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg
    21901 tagtttattg gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt
    21961 gataagcggg gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt
    22021 ggccgggaaa accaaaatta caggtaatag tttatgacaa tttcattgat gatgttcatg
    22081 aataattatt gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa
    22141 acagaaggcg gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt
    22201 ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa
    22261 catccccaat agtggcagga aagcacagat tttatagact aactcgatgc t
//
LOCUS       DAZOXR010000059        21955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZOXR010000059 DAZOXR010000000
VERSION     DAZOXR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.061"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            90..563
                     /gene="dfrA1"
                     /locus_tag="ACHUNN_001930"
     CDS             90..563
                     /gene="dfrA1"
                     /locus_tag="ACHUNN_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0576317.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            658..1182
                     /gene="sat2"
                     /locus_tag="ACHUNN_001931"
     CDS             658..1182
                     /gene="sat2"
                     /locus_tag="ACHUNN_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0576318.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1251..1748
                     /locus_tag="ACHUNN_001932"
     CDS             1251..1748
                     /locus_tag="ACHUNN_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576319.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1809..2180
                     /locus_tag="ACHUNN_001933"
     CDS             1809..2180
                     /locus_tag="ACHUNN_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576320.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2590..2967
                     /locus_tag="ACHUNN_001934"
     CDS             2590..2967
                     /locus_tag="ACHUNN_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576321.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            2990..3142
                     /locus_tag="ACHUNN_001935"
                     /pseudo
     CDS             2990..3142
                     /locus_tag="ACHUNN_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3198..4814)
                     /locus_tag="ACHUNN_001936"
     CDS             complement(3198..4814)
                     /locus_tag="ACHUNN_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0576322.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(4815..6341)
                     /locus_tag="ACHUNN_001937"
     CDS             complement(4815..6341)
                     /locus_tag="ACHUNN_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0576323.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(6344..8011)
                     /locus_tag="ACHUNN_001938"
     CDS             complement(6344..8011)
                     /locus_tag="ACHUNN_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0576324.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(8008..10116)
                     /locus_tag="ACHUNN_001939"
     CDS             complement(8008..10116)
                     /locus_tag="ACHUNN_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0576325.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(10103..10924)
                     /locus_tag="ACHUNN_001940"
     CDS             complement(10103..10924)
                     /locus_tag="ACHUNN_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0576326.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(11084..12913)
                     /gene="glmS"
                     /locus_tag="ACHUNN_001941"
     CDS             complement(11084..12913)
                     /gene="glmS"
                     /locus_tag="ACHUNN_001941"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0576327.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(13075..14445)
                     /gene="glmU"
                     /locus_tag="ACHUNN_001942"
     CDS             complement(13075..14445)
                     /gene="glmU"
                     /locus_tag="ACHUNN_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0576328.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(14796..15215)
                     /locus_tag="ACHUNN_001943"
     CDS             complement(14796..15215)
                     /locus_tag="ACHUNN_001943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0576329.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(15236..16618)
                     /gene="atpD"
                     /locus_tag="ACHUNN_001944"
     CDS             complement(15236..16618)
                     /gene="atpD"
                     /locus_tag="ACHUNN_001944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0576330.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(16645..17508)
                     /gene="atpG"
                     /locus_tag="ACHUNN_001945"
     CDS             complement(16645..17508)
                     /gene="atpG"
                     /locus_tag="ACHUNN_001945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0576331.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(17559..19100)
                     /gene="atpA"
                     /locus_tag="ACHUNN_001946"
     CDS             complement(17559..19100)
                     /gene="atpA"
                     /locus_tag="ACHUNN_001946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0576332.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(19113..19646)
                     /gene="atpH"
                     /locus_tag="ACHUNN_001947"
     CDS             complement(19113..19646)
                     /gene="atpH"
                     /locus_tag="ACHUNN_001947"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0576333.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(19661..20131)
                     /gene="atpF"
                     /locus_tag="ACHUNN_001948"
     CDS             complement(19661..20131)
                     /gene="atpF"
                     /locus_tag="ACHUNN_001948"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0576334.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(20193..20432)
                     /gene="atpE"
                     /locus_tag="ACHUNN_001949"
     CDS             complement(20193..20432)
                     /gene="atpE"
                     /locus_tag="ACHUNN_001949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0576335.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(20479..21294)
                     /gene="atpB"
                     /locus_tag="ACHUNN_001950"
     CDS             complement(20479..21294)
                     /gene="atpB"
                     /locus_tag="ACHUNN_001950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0576336.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(21303..21683)
                     /gene="atpI"
                     /locus_tag="ACHUNN_001951"
     CDS             complement(21303..21683)
                     /gene="atpI"
                     /locus_tag="ACHUNN_001951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0576337.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         5655 a         5652 c         4954 g         5694 t
ORIGIN      
        1 acaagtgatt atattaatta acggtaagca tcagcgggtg acaaaacgag catgcttact
       61 aataaaatgt taacctctga ggaagaattg tgaaactatc actaatggta gctatatcga
      121 agaatggagt tatcgggaat ggccctgata ttccatggag tgccaaaggt gaacagctcc
      181 tgtttaaagc tattacctat aaccaatggc tgttggttgg acgcaagact tttgaatcaa
      241 tgggagcatt acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt acatctgaca
      301 atgagaacgt attgatcttt ccatcaatta aagatgcttt aaccaaccta aagaaaataa
      361 cggatcatgt cattgtttca ggtggtgggg agatatacaa aagcctgatc gatcaagtag
      421 atacactaca tatatctaga atagacatcg agccggaagg tgatgtttac tttcctgaaa
      481 tccccagcaa ttttaggcca gtttttaccc aagacttcgc ctctaacata aattatagtt
      541 accaaatctg gcaaaagggt taacaagtgg cagcaacgga ttcgcaaacc tgtcacgcct
      601 tttgtaccaa aagccgcgcc aggtttgcga tccgctgtgc caggcgttag gcgtcatatg
      661 aagatttcgg tgatccctga gcaggtggcg gaaacattgg atgctgagaa ccatttcatt
      721 gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct ttgaactatc taccagaagt
      781 gtgagcccct accggaagga ttacatctcg gatgatgact ctgatgaaga ctctgcttgc
      841 tatggcgcat tcatcgacca agagcttgtc gggaagattg aactcaactc aacatggaac
      901 gatctagcct ctatcgaaca cattgttgtg tcgcacacgc accgaggcaa aggagtcgcg
      961 cacagtctca tcgaatttgc gaaaaagtgg gcactaagca gacagctcct tggcatacga
     1021 ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt acgcaaaatg tggctttact
     1081 ctcggcggca ttgacctgtt cacgtataaa actagacctc aagtctcgaa cgaaacagcg
     1141 atgtactggt actggttctc gggagcacag gatgacgcct aacaattcat tcaagccgac
     1201 accgcttcgc ggcgcggctt aattcaggag ttagagagct ggggaagact atgcgcgatc
     1261 tgttgaaggt ggttctaagc ctcgtacttg cgatggcatc ggggcaggca cttgctgacc
     1321 tgccaattgt tttagtggat gaagctcgtc ttccctatga ctactcccca tccaactacg
     1381 acatttctcc aagcaactac gacaactcca taagcaatta cgacaatagt ccatcaaatt
     1441 acgacaactc tgagagcaac tacgataata gttcatccaa ttacgacaat agtcgcaacg
     1501 gaaatcgtag gcttatatat agcgcaaatg ggtctcgcac tttcgccggc tactacgtca
     1561 ttgccaacaa tgggacaacg aacttctttt ccacatctgg caaaaggatg ttctacaccc
     1621 caaaaggggg gcgcggcgtc tatggcggca aagatgggag cttctgcggg gcattggtcg
     1681 tcataaatgg ccaattttcg cttgccctga cagataacgg cctgaagatc atgtatctaa
     1741 gcaactagcc tgctctctaa taaaatgtta ggcctcaaca tctagtcgca agctgagggg
     1801 aaccactagt gtcatacgaa cctccaagag acggttacac aaacgggtac attgttgatg
     1861 tcatgtatga caatcgccca agtaagtatc cagctgtgtt cagaacgtac gtccgaattc
     1921 cagactatgt cgaactagat aacgcggagg agctggccga cgcaatcatt cgagaggttg
     1981 agttaaagga ggcatggctc acaagggtct tggggaactc agccgatcct gacgcttctg
     2041 tgggcaagtg ggaccggcgc ctagcgcagt gcggtggtat tgatgagatg tcaagtgggg
     2101 tgcaggcaac atacttcagt ccaaacggtc agctggtcgg cttagtggca tacattgatg
     2161 ccgaatggat ggatgaatag ctctgacgtt acgattcagg tgagacaacc agatgggcat
     2221 tgcgtacaag ctcagttgcc accgatgtgg ctacgagtct gatcttcttt acctaggcca
     2281 aggtatggcg atgcttcctg agcaaatagt cggaacgtgc acatgctgcg acaatctaac
     2341 gacgatttct ataaatgaaa gctacggagt atgctcactt tgtggggcta aggatcgtgt
     2401 gatatttaca agctatcagc atgaggcaag gccaagaaat ccctgctatc tagagagaag
     2461 tttcaaatat caatgcccaa gatgccatgc cttcagtatg gagcctccgg atctaccgga
     2521 gatgctttgg gactaatgct ccgctttgcc tcgggtatcc gttcgaccta tgtttaagag
     2581 gaaacgcacg tgtctgccgc caaagctctt gcctatgcac tttgtgacaa gttggaaagc
     2641 aaaaaagttg atgaagtcgt acgaaatctt gtttctagcg gctggaatat tagggaagtg
     2701 gcctgggaag cttcgtcctt tgatcatgaa atgccctact ttgacacggg gcgggacttc
     2761 gatattggat ttcctgacac gctgtcatcc aagattgagt ttttgaccgc gaatggatca
     2821 atcgtccaag cacgtgctcg cctgcacttc aagcaggctc taatttttgc caaggcgtcc
     2881 aaacatgtcg ccgcgctcaa gaactcattg gattactact atggatcgtc tgtaatcccc
     2941 cctcaaaacc acggaggcta tttgtagaaa attccattaa tatgaactca aggtgttccc
     3001 tacggacgaa tcagcgatga aggtggtgta cctggctatc caggcggcga ccaagaaatg
     3061 gacgatgccg atccgcaact ggaagccggc catgaaccgt tttatgatcg aatttggtga
     3121 ccgtttaaat ggccaccttt aactgaccgg caaaagcact tacacaaaag ggtttacagg
     3181 ctctttcagt cattccttta atgcgtaaat tgctctctaa ccactctctc agcccaccct
     3241 tccatatcct ctctcgatac ttgcagctcc cccaacccct ttggatgatt cacccctcta
     3301 tgcccgtctt gtccatataa ttgatcaaac aaactatttg gccaattcaa tgagctcttc
     3361 accactccac gccgtatctt ttcaaaatca ttccgccatg tttcgctatc aacgccactc
     3421 aacactttgg tagaaagcat tttcacccca tccgatgcat ccacctctag caatacaaat
     3481 tcactgccat tacgttgcac cccaacggct ttaatcactc ttggtgagcc atctttacac
     3541 agatgtaatc ggctacgccc aacttttggc aaaaccaagg tttcttcaaa aagcacccga
     3601 catgcaaatt tagtttttag aattgaaagt agctcatcaa aagcagcaaa tcgattagca
     3661 aaaatagagt tgtaattggt tgcatcctgc ttcccaccca catctgccgc tgctaggaca
     3721 ccacctaagt gtggctcatc agtactaacc aaattactat tttcacttgt tgttgcttct
     3781 tctttttcta aaatagtttg gctacttttt cttgccctgc gagatttttc aattcgacta
     3841 ggtctactaa aacttatcca tgtcggctca gcctctatga ctcgtgtgtc tgtgtctata
     3901 ttggctgttt cctcatcgtc caactgtaga tgctcatcat caacaactga ctctgttgaa
     3961 accgctatgt gctgcgtact accatcacct gcctcctttt cctgaaaaga ggcatggcta
     4021 atagctgttg tgctaggaag catagcgttg atttctaaac ctactatttc ctcaactaaa
     4081 taatccttat cctcgttaga agaacgtcct tttacatgta atttccaacc ctgcatgggc
     4141 ggagggtcaa agctaaagca ccagctttca acgccgttct taatttctct attttgttga
     4201 tagtgcctaa aaatactttc atacgaatcc ataacatctt gatccgaaaa caaccaaacc
     4261 aaaagctgca ctacggccga ctgctctaac gcccctttag gaaacgatga actgggtaag
     4321 atccttatct ctaaatgatc tcgctcaacc tcatactgaa cgtcgaactc ttgctgtaaa
     4381 gcggtactgc tcaaacagct tcgacagaaa taggagttaa ttaaaaataa tgaccgagct
     4441 agctctaact gtgggatgtg ataacgagtc ttgctatatg gtgtttcagc ttcgtaaata
     4501 aatgaactct gcgctccatc cttgttgcgt acggaagcaa aggacgagag attaggaaaa
     4561 tcgattattt tggctcgttg aagatccgat gcttcaaact caatcacacg atccgcctta
     4621 tttatttgct ttgttgtact gttaaccacc ttagctcgac ttaataaagg caaatgagta
     4681 aatcgagtcc atttccgttc ttgcattgga ttaaaccaaa caaaaatacg ccactcctta
     4741 tgacccgagt tacggaataa atgaccaata tgtacaacct gcacattgtc attaaatgta
     4801 gctagcctaa ccactcactc ttccccataa accatttcca agaatctttg tgcttcctca
     4861 gttatccgct ctttagataa cgttgcactt cttaataatc gccagcgcct aagctcaaca
     4921 tcttcctgtt taagcttaat aaaagcttgg ctaatccgtc taatttgata atcttccaca
     4981 ctctctgagt aacgctttaa gcaaagcgct accaaaggca gtttctgtag attttttgca
     5041 agagaggttc cgttaggagt ttgctttaac agccagctcg atgtcgctct tgggtgatca
     5101 aggctactat ctagacgctt tatgattctt aatagttgtc gtacagcaat tcgatctctt
     5161 tggttccaat caactctagg ggcgggtgcc agacgctctt gttgatgctg ttgattccag
     5221 tgaactagcc aatccctgtc atgtcggtaa agccaagcgt atagcacccc accctctaaa
     5281 gactgtcttg ccgccttaat tccttggtat ttatgcacta gttgctgcca gtctttatgc
     5341 ttaacagata aatcttcaga gttaggttgc acagactggc taacaggtct tgtcgttata
     5401 gagtgctcag taagagcact tgcctgctgt agcgcttcta taaccgttag ttttggcaat
     5461 aaggcttgcc acacaatact atgctgtaaa taactaaagg cttttctatg cttacggaat
     5521 atacttttca gccaacacgt gtccttgttc tctgccaact ttaaatccag tttctctagt
     5581 gcctcatcac taaaagtctg cctcactctc tccgccacca agtcatgacg aatgtgcttg
     5641 cttttggtta gccctagatc ctgcgctaag cgctgataaa acagcgtcca ctgctcaagg
     5701 cttggggaaa gctcttgcgc tcgtggagca tctaacagag gggctatata agcagctagt
     5761 gctgttaatt gagataggga gtctttgggg tagtctgaaa gcagctcagt atgacccaaa
     5821 gcccaaaatt gatgtcggtg atcatctaca gctctatcaa agaagactaa agcaccgtgt
     5881 tttggacaat atggcaaagc gggcaaatac caatctcgtt gccaaaaggc ttccccatac
     5941 ctatttagct gaagagcaac gcaatcaggg cagtatctaa agcggttatc gctcttaact
     6001 ctagaagcag cgactcctag cattaaatgc accgcacctt gcgcttggta ctccattaac
     6061 cgaatagctt cgtctcggcg ctccttgcct acaaacggag catataaagg gaataaggta
     6121 tgctcataaa taagctgctg aacagcgtaa cgtcctgttt gatgtagatg tcttgctatc
     6181 acacctaaat gcgagggcag acctaaggta gcgaccacct tgcggttgcc atacacctca
     6241 tccaacagct gcttaggact aacaatccct tgataaacgc ctgcccgtgc aatagtgcta
     6301 taaatcagct cattcgagta cggaacagga aagtttctca tggctaacct gcttgtttaa
     6361 ataagctcgc catatccact attacccctt tcccttttaa ccgttcatgc atggtttttt
     6421 caggttggcg ttgtgaatag atataacgta aatccgtatc aggcaagcta tcccagtcgc
     6481 tgggcttgac tacctttata gccgaaacct ttttactctt ggagggcttt tctagttctg
     6541 acactaccgt ttcgccttcc atcaaccact gcaaaacaag aggcagtaac ttttgtcttg
     6601 tcatcgttgg attctggcta aacgcttttt taatagtggg aattaacagg cttgaatcgt
     6661 aatcctcttt cagcatcaga tataaatgac gctgatcttc ggtatctaac tcttgaaggg
     6721 ctttttcttc tggtgtttgt tcttgtatcg ctgcgatatc tagctgaagt tggattaacc
     6781 gtttatcaat ctcgggaacg actagatcag aataacgagc aatgcgttct gggatacccg
     6841 agcgtaatgc ctctagcatg gggtgcacag gctttaactc atcttgatac acttgccgca
     6901 ataaaccagc ggtaatacgc tcattgccta aagctagcgc acggagctga gcgagtacaa
     6961 aaagttttac tacaatgtcc atcactcctt ggcttagctc ataccacaca tcacggacct
     7021 catccgataa cagcgcatct ttgcgttgta aaagctgtaa ttgccaaaga ttatccgtaa
     7081 aagcgatcca ctcttgattg ggctttccac gttgcgtttg ttgtatagga tcccagaata
     7141 tagctccaaa ccctgcccct ctacgtgcag accgcaaatc agcctcaaaa atctctcgtg
     7201 ctttaggggt accaatcaac atcactggta cgccaataat attcaccatc gtcacaaaaa
     7261 agttcagcat ctcttgagat ccacccgaac gagagcggct taaatgctga atttcatcaa
     7321 taaccaacaa ccctaaagca tgtgcattgg ctatttgcga catcaaagcc aacatggttt
     7381 ctataccatg acgttttaag ccataacgac gctcatagtt cgagcccaag gctcgatcca
     7441 acgctctgaa aaaattcaag cagatttctt ttagcgaacc attatgcgag cagtctattt
     7501 tcaaatacac cacctgctct acattgagtt cacgatggta aatcacctga ggatacgtgg
     7561 ctagaatacg atgaagagag gtcgtcttcc cactaccaga acaaccaatt aacaataagc
     7621 tttgtgccgt agatcgtgcc tcctcaaagc gaaatgtctc caactctccc gtttgaacac
     7681 gctcataacc attttgtaaa tgcttttgta aatctcctgt tttaggattt ctgcctacgt
     7741 agccacctcg aatcatgacc gaaatacgct cacttagtag caaatgcgta cctaatggct
     7801 gaaaatagtc atctggaata cgacaaatgg tatgagctct gataacacgg gacttttgca
     7861 agtcagagga agtaagctgt aaagaggatt tcagtgatgc agcactattc actgactctt
     7921 gtaatggtgg taaggcctcg ataaaaggat tatcacgata agcctctacc cccgtatcac
     7981 gataaactgc ttgaatccgg gtagcactca tgactcatcc ttttctggtg gatcctgaaa
     8041 taattcaggc acgtatgtgg gtaggctgta atcttcttga tcatccgctt ccactgcgtt
     8101 gaaaggaata actttagcct catcacctga agagcttggc ttcaaatgct ccgcacgttt
     8161 tttacgctcc gaggtcacgg cttctttttt attagtttta atctgcttaa tgcgtgttga
     8221 tttgggctca gtagtactgg gcgttaactt attcgctttc tgaatggttt gctgaataaa
     8281 cgcctcaagc tccctgcgtt tagttaactc atcctgcttc gcattggctt tattgtgttt
     8341 ttcttgtgct tgtatatccc aaacctccca aaatgagaga cctttaaact gccgactacg
     8401 ttccgtcaga ttacagcgcc aaaatacacg gctgccaact tgcggaaaca aataaatcgt
     8461 atcaaccagc actgggtcat aagccgcttc taaatgttga ggtctagcta tatcagtgct
     8521 ccgctgcaac caaccctcac gcagaatctc tgaccccgag taatacaaac cccacaaatt
     8581 aacgccaaat gaagaaatag agacctttcg gcgaggcagt aacgctactc gcaactgctc
     8641 ttgctccaca gcccttaaac tacctgtacg atgctgcata ccccattgcc atagctggac
     8701 aggaatagac ggtaaatctg taggaaaatc agcatctcga tcgtatttat ccatcaccag
     8761 atggttattt ctgaataaga tcgtacgcaa aataatttgt gtgaactcaa ataccgacag
     8821 agatgcatct aacctatagt ctgtttcacc atggcttttg atccgactgc cctctacaat
     8881 gccaggtgca aaggacttaa actcggcttg tagtgttcta aaagtgcttt ccactatgcc
     8941 tttagcatcg ccacgtctag gtggagcact ttccactcgc acattaaaac tagaaactaa
     9001 ggcttcgacc tgatgactca ttaattcgcc acggtccgct agcaacacat ctggcaaacc
     9061 tacacacggc cagtctgagc tactaatctc aatatcatgc tgggcacaaa tggccgtttt
     9121 gtcagagcaa gcatttacaa aagcctgcat cgccaccaca taagacggat tttcaaagcc
     9181 gatataaaag cccgtgatca tccgactaaa cacatcaatc acaatgtaaa gcgttggtct
     9241 tcctatgatt ttttggcgat catgatgatc cactaaataa atatcagcaa tcgtggcatc
     9301 aatctcataa cgactcccag ggcctaacgc ctgagaagtg gctgtactac ttaagggtcg
     9361 tacgtctttt ttatataccc ctgctttaac tctagacttt aagcgctgag ctttagggta
     9421 ttctcgatca taaaaataac gaaactgacg tagtgttggg taatcctctt ggggaatgcg
     9481 aggaaaatac tgagcaaaca agtccacaaa tcgtctatag gcaacggtgg tctttgtacc
     9541 tttttgattt aacaggtgct tttctatggt caacctaaaa aggcgttcaa tctcgggcgt
     9601 taccttggtt ccttcaccct taccatattc tctggctcgg ccaatctttg ctgttcctgt
     9661 cgctgaacgt ctttcccctg gtgcaccgct gtttttgtag tcaggaatta atgcattagg
     9721 cgtttgacca cgctgccagt aacggcgtaa caacttataa actgtagcct tagtaacctt
     9781 atgttcttgg accacatgct caacgagttc gcttctgact ttagggtcga aacgatcctt
     9841 actattaata ataggaagaa tttttcgata atcctcctcg cgcttctgga agctgacaga
     9901 atctacagac ggctcttcta aatcaagatg tacataaggg tcatcagttc taactaagcg
     9961 gccttcatct aaatactgca ttagcaacaa ctcagcccta gcttgtggaa ctcctttatc
    10021 agcgcttatt tgcatccaga caacttggcc atcctctata gccaaaatgc gatacggatc
    10081 attatcaaat agcacaacct cattaatttg ccacatagcg caactcctcc atattcacta
    10141 cttggctaat acagagatct gcacacttat ttgccctgaa agacttataa atattgaact
    10201 taataaaacc atttgcggtt aaggctcgta tctcactcaa tgttttgcct aactccaaat
    10261 cataagcaat atcaacctgc ttacagacat tgataatgtt ttcatctcct ttttcttgca
    10321 ggatatgggc caatggggat agttgtgcta aaagctccgc agaaacttct tctgttttca
    10381 ctgaataaag ccattcaata ttttctttta ctacgggatt tatttcttta tcagtaaaaa
    10441 tgaaccaagg aatttgcttt tgctgccaat agcgacgctc tagttctagt ttttctaagg
    10501 tacgctcgtc ttgtaaggct gctgcaggtt tgacttgaat agcaaactgc tcaaaaggac
    10561 catctttgca gtccactaaa aaatcagtag acataacctg atctacacca cgaataacag
    10621 gatgcttaat accactatct attgcaatct gcctggtatc actaggtaat aaggggaact
    10681 gctcgcgtat atctagcacg ctgctctccc actcaagact gagaaaaaca gcaagctcta
    10741 agtcagatag caaatgatgg actcgtcccg tcttatgaga ataaatacgg tgggaacgac
    10801 ctgaagaagg aacttcttgt actgttagcc atggaatata gtctttacca tgcccttggc
    10861 cacgcccctc tttaatacga cgggcaattt gcacttcaga aaatgaagag tttgctttag
    10921 ccataacaaa agtccagtat gctttttcac agcataactg gactgatttc agtttacaac
    10981 tattctgtct agtttaagac tttattgtca tagtttagat ctattttgtt cagtttaaga
    11041 ctttattgtc cgcccacaag ccctttttta tctatgatag tgattactca accgtaaccg
    11101 attttgccag gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg acatggtaag
    11161 ccagcagctg cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc acatgcggca
    11221 tctcgatgat gtgcatgtta tcgctactta caaaacccgc atcctgatcg gcgaagacat
    11281 acaattggcc gccacgcgcg cgaacttctt caatgttgga tttcagtttt tccagcaatt
    11341 cgttgttcgg tgcaacgacg ataaccggca tatcggcatc aataagcgcc agcggaccgt
    11401 gtttcagttc accagcagcg taggcttcag cgtgaatgta ggagatctct ttcagcttca
    11461 atgcgccttc cagtgcgatt gggtactgat cgccacggcc caggaacagt gcgtgatgtt
    11521 tgtcagagaa atcttctgcc agagcttcaa tgcgtttgtc ctgagatagc atctgctcaa
    11581 tacggctcgg cagcgcctgc agaccatgca caatgtcatg ttcaatggag gcatccagac
    11641 ctttcaggcg agacagcttc gccaccagca tcaacagcac agttaactga gtggtgaatg
    11701 ctttagtgga cgccacgccg atttccgtac ccgcgttggt catcagcgcc agatcggatt
    11761 cgcgcaccag agaagaaccc ggaacgttac agattgccag cgaaccaagg taacccagct
    11821 ctttcgacag acgcaggccc gccagggtat ccgcggtttc gccagactgt gacaaggtga
    11881 tcatcaggct gttacgacgc acggcagatt tacgatagcg gaattcagag gcgatttcga
    11941 cgtcgcacgg aatacctgcc agcgattcaa accagtagcg ggaaaccata ccggagttat
    12001 aagaagtacc acaggcgagg atctgaatat gctcaacctt cgacagcagt tcgtcggcgt
    12061 tcggtcccag ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta agggtgtttt
    12121 ttatcgcgtt cggctgttcg tagatctctt tctgcatgta gtgacggtaa atacctttat
    12181 cgcccgcgtc atattgcaga ttggattcga tatcctgacg ttttacttcc gcgccagttt
    12241 tatcgaagat gtttaccgaa cggcgagtga tttccgcaat atcgccctct tcaaggaaga
    12301 taaagcgacg ggtcaccggc aacagcgcca gctggtcaga agcgataaag ttttcgccca
    12361 tacccaggcc aatcaccagc ggactaccag aacgtgccgc cagcagggta tccgggtgac
    12421 gggagtccat gatcactgta ccgtacgcac cgcgcagctg cgggatagca cgcagaacag
    12481 cctcacgcag agtcccgcct tgtttcagct cccagttcac cagatgggca atcacttcgg
    12541 tgtcggtttc agaaacgaag gtatagccac gcgcttttaa ctcttcacgc agcggttcat
    12601 ggttttcgat gatgccgtta tgcaccacca caatgtgttc agaaacatgc ggatgcgcgt
    12661 tagcttctga aggttcaccg tgggtcgccc agcgagtgtg agcaataccg gtgccgccat
    12721 ccagaggatg ttcttccgct gcctgagcca gcatctggac tttaccgagg cgacgcaggc
    12781 gggtcatatg accttccgca tcaacaacgg ccagaccggc agagtcatat ccgcggtatt
    12841 ccagacgacg taaaccttca agaaggattt ctgctacatc acgttgcgcg atcgcgccaa
    12901 caattccaca catagttttt gattccgatt tatatcgttg tcggtcaacc tgtatgcccg
    12961 ttttttcggg cgccccgagc cttgtagaga gtggggttat ttttatagtt actgcttgtg
    13021 ggagggagat tatgttatct cctcatccca gatgaccgga ttacccggcc agaattactt
    13081 tttctttacc ggacgacgcc agccttcttt ctgagtctgc ggcacacggc tgatagctaa
    13141 tgcattttcg ccgacattac gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc
    13201 tactgttacc ggggccacca gctgagtgtc ggaaccgaca aacacatcgt cgccgataat
    13261 ggtcttaaat ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac
    13321 gttatcgcca atttccgcat cgcccaggta agtcagatgc ccgactttcg agcctttacc
    13381 cagacgcgct tttttcatct caacgaagtt accaacgtgt gcaccttcca gcaactcagc
    13441 accaggacgc aaacgagcaa acgggccaat agtacaggcc gctgccagat tcgcgtcttc
    13501 cacaacggta tacggactga tttcgcaatc atcgccaatc acgctgtttt taatcacgca
    13561 accggtgccg attttcacgc gatgaccgag agtcacgttg ccctcgatga taacgttagt
    13621 atcaatttca acatcgcgcc cgtgagtaag cgtaccgcgc agatcaaaac gcgccggatc
    13681 gcgcagcata acgcctgcta acagcagttt ttcagcctgt tcggactggt aaacgcgctc
    13741 cagacgggag agttgcaggc ggttattcac accttctact tcgcttaaac gttgcggatg
    13801 aacggcgacg atttcacgcc cttcctgata cgccagcgca ataatgtcgg tgatgtagta
    13861 ttcgccctga gcattattgt tggtcagctt cgccagccag cgtttcatat ctgcgccgtt
    13921 agcgatcaga atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg tggcatcttt
    13981 gtgctcaaca atgccggtaa ctttgccatt ttcacgggtg atacgtccat aaccggtcgg
    14041 atcatccagt ttcaccgtca gcagaccaat gccaccctgc ggtttagcat cacgcaggcg
    14101 ctggagtgtt tcgacagaga tcagcggcac gtcgccgtag agcattaaaa tgtcttcatc
    14161 atcggcaaag aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct gctctgcctg
    14221 aagcacccag ttaaggttgt cgtctttcag cgcctgttta agcagatcgc cgccgtgacc
    14281 gtacaccaag tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga catgctgaac
    14341 catcgctttc ccggcaaggg tatgcagcac tttcggaaga tcggaataca tgcgcgtgcc
    14401 tttgcctgcg gcaaggatca ctacgctcat agcattattc aacatacgcg tcctgactgt
    14461 aatttgagaa cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt tcatcgtgaa
    14521 atggacagag gataaattgt tcaataacgg attatccccg ctgacgaaag tgccattttc
    14581 gaccatcgtc acgccttttg tctgcagaaa attaaggcag taccttcatt taagcgtcag
    14641 gtggatgttt ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg gtgttttata
    14701 tcagcaggat ctatgtgaac gctcttcagg acggatcacg cgtaaaaaaa agccagcctg
    14761 tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg
    14821 ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg
    14881 tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct
    14941 tcgtcgagat cctggccgcg aattgcggtg tcggccagaa cggtcacgtt gccaggctgc
    15001 acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg
    15061 cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt
    15121 tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct
    15181 gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa
    15241 gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg
    15301 gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg
    15361 agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga
    15421 agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca
    15481 tacccaagat ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc
    15541 cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg
    15601 agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca
    15661 cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag
    15721 gtacgtatac cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct
    15781 gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc
    15841 ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca
    15901 gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa
    15961 cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga
    16021 taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta
    16081 cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca
    16141 tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga
    16201 acggacacat caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca
    16261 gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc
    16321 ctttcatgtc gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta
    16381 ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg
    16441 aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca
    16501 ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct
    16561 gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct
    16621 taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag
    16681 ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag
    16741 gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc
    16801 caggttttca accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc
    16861 cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc
    16921 atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt
    16981 aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aagcctgcaa
    17041 catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac
    17101 ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat
    17161 tgcgaggtcg cattgaacgc ttttgtcggt ccaggtcttc atttccgcca gtagtttttt
    17221 gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc
    17281 cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc
    17341 aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc
    17401 ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt
    17461 gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc
    17521 aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg
    17581 aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg
    17641 ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg
    17701 aagctgccaa ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga
    17761 accagagact gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc
    17821 actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga
    17881 gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt
    17941 ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc
    18001 gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg
    18061 gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc
    18121 agtgcggtca gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat
    18181 tcggcgttaa cacgtgcagc acgctccagc agacgagagt ggaggtagaa aacgtcgccc
    18241 gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca
    18301 gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat
    18361 tcgcccattg cgcaaccggc atacggtgcc aggtattgca gtgcagcgga ttcagacgcg
    18421 gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg
    18481 ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg
    18541 cgctggttga tgatggcatc gatagccagt gcggttttac ctgtctgacg gtcaccgatg
    18601 atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc
    18661 tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca
    18721 gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc
    18781 acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact
    18841 ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc
    18901 tcaaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata
    18961 caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg
    19021 ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt
    19081 tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc
    19141 tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct
    19201 gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc
    19261 atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct
    19321 acctcagcgg tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg
    19381 agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc
    19441 agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca
    19501 gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc
    19561 tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg
    19621 cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt
    19681 tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc
    19741 cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg
    19801 cttcaatttc tgcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt
    19861 tcgcttcgtc cagaatctgc gagcggcgtt tgttcgcctg ctcgatgatt acctgggctt
    19921 ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat
    19981 gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg
    20041 ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg
    20101 cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc
    20161 tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga
    20221 cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga
    20281 gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg
    20341 aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc
    20401 atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa
    20461 cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag
    20521 aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac
    20581 attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc
    20641 accggcatac atgttaccga acagtcgcaa accgagtgaa actggtttgg acagcaggct
    20701 taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt
    20761 caactctttc gtgaagccgc cgatgccttt cattttgatg ctgtagaaca gaatcaggat
    20821 aaatacgccc agtgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag
    20881 tgcaggcaga cccagtacat gttcagcaat gtacggcagc aggtcgatag gcagtaaatc
    20941 catcaggttc atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt
    21001 gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat
    21061 cgcggtctga aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa
    21121 aaccaggaac aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca
    21181 gaaggtggct ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag
    21241 gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc
    21301 ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc
    21361 cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac
    21421 accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc cacccggcct
    21481 ttcgctggtg tatacgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga
    21541 aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac
    21601 agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt
    21661 cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc
    21721 gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata
    21781 cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat
    21841 ttttccagtg catacaattg cgacttttcc gctaaccctg ttcgatcatg aaaaactgta
    21901 aataacgcgt aaacactagt gataaagcgt gcttcagatc acatattgcg catgt
//
LOCUS       DAZOXR010000060        21641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZOXR010000060 DAZOXR010000000
VERSION     DAZOXR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.062"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="ACHUNN_001952"
     CDS             complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="ACHUNN_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0576338.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1343..2995
                     /gene="ushA"
                     /locus_tag="ACHUNN_001953"
     CDS             1343..2995
                     /gene="ushA"
                     /locus_tag="ACHUNN_001953"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0576339.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="ACHUNN_001954"
     CDS             complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="ACHUNN_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0576340.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3715..4509)
                     /locus_tag="ACHUNN_001955"
     CDS             complement(3715..4509)
                     /locus_tag="ACHUNN_001955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0576341.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4647..4988
                     /locus_tag="ACHUNN_001956"
     CDS             4647..4988
                     /locus_tag="ACHUNN_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0576342.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5203..7707)
                     /gene="copA"
                     /locus_tag="ACHUNN_001957"
     CDS             complement(5203..7707)
                     /gene="copA"
                     /locus_tag="ACHUNN_001957"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0576343.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7969..8901
                     /gene="glsA"
                     /locus_tag="ACHUNN_001958"
     CDS             7969..8901
                     /gene="glsA"
                     /locus_tag="ACHUNN_001958"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0576344.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8904..10196
                     /locus_tag="ACHUNN_001959"
     CDS             8904..10196
                     /locus_tag="ACHUNN_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0576345.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10227..10324
                     /locus_tag="ACHUNN_001960"
                     /pseudo
     CDS             10227..10324
                     /locus_tag="ACHUNN_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10321..10728
                     /gene="cueR"
                     /locus_tag="ACHUNN_001961"
     CDS             10321..10728
                     /gene="cueR"
                     /locus_tag="ACHUNN_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0576346.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10729..11187)
                     /locus_tag="ACHUNN_001962"
     CDS             complement(10729..11187)
                     /locus_tag="ACHUNN_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0576347.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11184..12101)
                     /locus_tag="ACHUNN_001963"
     CDS             complement(11184..12101)
                     /locus_tag="ACHUNN_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0576348.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12247..12924
                     /gene="fetA"
                     /locus_tag="ACHUNN_001964"
     CDS             12247..12924
                     /gene="fetA"
                     /locus_tag="ACHUNN_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0576349.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12911..13690
                     /gene="fetB"
                     /locus_tag="ACHUNN_001965"
     CDS             12911..13690
                     /gene="fetB"
                     /locus_tag="ACHUNN_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0576350.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="ACHUNN_001966"
     CDS             complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="ACHUNN_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0576351.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="ACHUNN_001967"
     CDS             complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="ACHUNN_001967"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0576352.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="ACHUNN_001968"
     CDS             complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="ACHUNN_001968"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR0576353.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16061..16747
                     /gene="ybbA"
                     /locus_tag="ACHUNN_001969"
     CDS             16061..16747
                     /gene="ybbA"
                     /locus_tag="ACHUNN_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0576354.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16744..19158
                     /gene="ybbP"
                     /locus_tag="ACHUNN_001970"
     CDS             16744..19158
                     /gene="ybbP"
                     /locus_tag="ACHUNN_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR0576355.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="ACHUNN_001971"
     CDS             complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="ACHUNN_001971"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0576356.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20555..21481)
                     /gene="allS"
                     /locus_tag="ACHUNN_001972"
                     /pseudo
     CDS             complement(20555..21481)
                     /gene="allS"
                     /locus_tag="ACHUNN_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5798 g         5144 t
ORIGIN      
        1 atcggcgata agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa
       61 aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat
      121 ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg
      181 caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat
      241 cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac
      301 ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg
      361 catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat
      421 gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat
      481 cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg
      541 cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc
      601 ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc
      661 gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg
      721 agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac
      781 tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc
      841 aattggcaac gaccgcggca tcggatattt atcggtccag tagccgacca ctggttgcag
      901 tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga
      961 aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag
     1021 cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga
     1081 cgcagccgcg cccgccacag gctggggttg ttcactcatt gccataggaa agtcactttt
     1141 tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt
     1201 atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt
     1261 tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg
     1321 aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt
     1381 aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa
     1441 aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga
     1501 atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga
     1561 aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga
     1621 cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc
     1681 gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc
     1741 caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa
     1801 accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga
     1861 tgacacagca aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc
     1921 cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat
     1981 cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg caccgggcga
     2041 tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc
     2101 gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac
     2161 gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa
     2221 atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca
     2281 gctgattccg gtgaacctga agaagaaagt gaccagggaa gacgggaaaa gcgagcgcgt
     2341 gctgtacacc cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca
     2401 gaacaaaggc aaagcgcagt tggaagtgaa aataggcgaa accaatggtc gtctggaagg
     2461 cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca
     2521 aatggatcgc actggtgccg actttgcggt gatgagtgga ggaggaattc gtgattctat
     2581 cgaagcaggc gatatcagct ataaaaacgt actgaaagtg cagccattcg gcaatgtggt
     2641 ggtgtatgcc gacatgatcg gtaaagaggt gattgattac ctgaccgccg tcgcacaaat
     2701 gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg
     2761 caaactcaac gacctgaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat
     2821 ggcgacatta aacttcaatg ccaccggcgg tgatggctat ccgcgccttg ataacaaacc
     2881 gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag
     2941 ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga
     3001 aagtgccgga tatttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt
     3061 tggcatcgag gatcttcgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc
     3121 cgccagaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gtcggcagac
     3181 gttttttctg ccccaatggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg
     3241 ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat
     3301 ccagttgacc tgcgaccggc gtaacggcca ctgccagatg tttcatatca ccgttcactg
     3361 ccaccagtag cgttttgtag acttgatccg gattcaaacc taattttttg acgacttcat
     3421 cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt
     3481 ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac
     3541 gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg
     3601 gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat
     3661 aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg
     3721 caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata
     3781 tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag
     3841 cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggagg
     3901 tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt
     3961 cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggtaatctg
     4021 gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaataa
     4081 ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag
     4141 aaccatcgcg atttgccaca gcggttgagt agaaaagagc gacggagaaa tgcccatctc
     4201 ctggctaatg tgctgcagat tttggagttg ctcctcgtta atgcgctctt ccagcgcctc
     4261 gcaagtaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag
     4321 cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct
     4381 acccatatga atactgccaa tcagatgaaa atggcgattg ccgggaaggg tgatatcgat
     4441 ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa
     4501 cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca
     4561 aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat
     4621 tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg
     4681 gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag
     4741 agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat cgtaaactca
     4801 ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa
     4861 acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat
     4921 tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg
     4981 tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc
     5041 cggcatccga gcaccattgc ctgatgcgac gcttacccgt cttatcaggc ctacaaacct
     5101 gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg
     5161 ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa
     5221 ccgcagcaac cggttggcgt tactcactac ggtaatcgac gagagcgcca ttgccgctcc
     5281 ggcaactacc gggttaagca gtgttcccgt aaacggccac aaaataccgg cggcgaccgg
     5341 aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt
     5401 tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat
     5461 cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg
     5521 ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact
     5581 ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc
     5641 aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac
     5701 cagacgatat cccgttttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg
     5761 atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc
     5821 tccttgcgat gcctgagcag taatctccgc ttcgatagct ttggtaccaa cctgttgctc
     5881 atttaacagc gcctgattgc ccagcaataa cgcatgacct tcagcttcac cgctcacgcc
     5941 cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc tgcatatcac ctgctttatc
     6001 gaggatcgcc cgtgccagcg gatggctgga accttgctcc agtgccgccg ccagacgcaa
     6061 tgcctgcgct tcatcaacat cagcaaacgt tttcactgcg acaacctgcg gcttcccttc
     6121 ggtcagcgtc ccagttttat cgaacacgat ggtatcgagt gtactggcgc gttgcagcgc
     6181 gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga
     6241 catcggcgtc gccagcccca gcgcacacgg acaggcgata atcagtaccg tggtggcaat
     6301 caccagggta taaacaattt gcggtgccgg accaaagaag taccagattg ccgcactgac
     6361 cagcgcaatc accaccacca caggtacaaa tacggctgag attttatccg ccagctgacc
     6421 gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccgtg acagcgtggt
     6481 atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc
     6541 atggacgctt tcgccttcgc ctttttgctg tgggattggt tcgcccgtca gcatcgcttc
     6601 atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat cgccggtcgt
     6661 caggcgcagt aacatccctg gctgaacttc cgccagcggc acgctttttt caccatctgc
     6721 ggtgaccact cgcgccgtcg gtggggttaa atcgagtaat ttctccagcg ccttagaaga
     6781 acgctgacgt gcgcgcgctt ccagcatatg gccgagatta atcagaccga taatcatcgc
     6841 actggcttca tagtaaagat gccgcgcttc catcgggaac cactgcggcc acaggttgac
     6901 gctcatcgaa tagagccacg ccacacccgt tcccagcgcc accagcgtat ccatcgtcgc
     6961 tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgccgg cgaaaaccat
     7021 cactgccagg gttatcaggc cgataactaa ccacaagctg cggttgtcag cggtgaccat
     7081 catgttatcg ccgatcatcc cccagaccat taccgggata cccacagcca gtgcaacaat
     7141 tgcctgccag cggaagcgct tcatcgtagc tacggcggtt tcttgctggc gctcgcggcg
     7201 tttagcgtca tcttcaatcg cttccgcacc gtagcccgct ttttccaccg cctgcactaa
     7261 atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc
     7321 ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca
     7381 gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag
     7441 cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc
     7501 ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc
     7561 tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgcct gctcaacatc
     7621 cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc
     7681 cagggtcagg tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg
     7741 tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta
     7801 aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt
     7861 tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac
     7921 cggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat gttagatgca
     7981 aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga
     8041 caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct
     8101 atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg
     8161 gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg
     8221 gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta
     8281 gagttgcatg gcggcaaacc gctgtcgcca ctggtaaatg ctggcgctat tgccaccacc
     8341 agcctgatta acgctgaaaa tgttgaacaa cgctggcagc gaattttaca tatccaacag
     8401 caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc
     8461 aacttccata accgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat
     8521 gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa
     8581 ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc
     8641 gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat
     8701 ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc
     8761 ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac
     8821 gaagaaggca acagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc
     8881 tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc
     8941 tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg
     9001 cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg
     9061 gcggtattgt ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca
     9121 gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag
     9181 cgctctcgtt gctgtacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt
     9241 ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc
     9301 tctacgcgtt ggggatcatt gcggtgatga cgctttttaa ctccttaagc aaccatgcgg
     9361 tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta
     9421 ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg
     9481 gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg
     9541 cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg
     9601 cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat
     9661 cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc
     9721 tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca
     9781 ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac
     9841 tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaac attattgtcg
     9901 tggtgttgat tatgctgatg acggcggcgc taaatttagg ctcactcgcc agtgttgcca
     9961 gcgccacctt tttgatttgc tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg
    10021 atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg
    10081 ttggctttat ctacagtctg tggtcccagg gtagccgtgc gttgatatgg attattggct
    10141 cactcttact cagccttatt gtggcaatgg tcatgaagcg caataaaacc gtataacatc
    10201 tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa
    10261 aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg
    10321 atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat
    10381 gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg
    10441 cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg
    10501 gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc
    10561 aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc
    10621 atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc
    10681 ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac
    10741 ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc
    10801 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
    10861 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
    10921 taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
    10981 ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg
    11041 caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc
    11101 atttccgccc agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg
    11161 tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc
    11221 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
    11281 actttgctgt tactggaggt accgatctgc tgtaacgctt cggtgtattt ctgcgctacg
    11341 aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg
    11401 cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
    11461 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc
    11521 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
    11581 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
    11641 actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg
    11701 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
    11761 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgcggc
    11821 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
    11881 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
    11941 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
    12001 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
    12061 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
    12121 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
    12181 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
    12241 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
    12301 gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
    12361 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
    12421 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc
    12481 taccgccaac aagtctctta ctgcgcccag acaccgacgc tgtttggcga tacggtatac
    12541 gataatctga tctttccctg gcagatccgt aaccggcagc ctgacccagc catttttctc
    12601 gattttctcg aacgcttcgc cttgccggac agcattttga cgaaaaatat cgccgagcta
    12661 tctggtggtg aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt
    12721 ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag
    12781 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
    12841 gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag
    12901 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
    12961 ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
    13021 ctggagcgtc ggacgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat
    13081 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
    13141 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
    13201 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga
    13261 atttatcccg atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc
    13321 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
    13381 ggagaaactg agtcttggtg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
    13441 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
    13501 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
    13561 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg
    13621 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa
    13681 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
    13741 atcaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
    13801 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
    13861 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
    13921 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc
    13981 cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat
    14041 ttgcgccacc atcccctggt agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac
    14101 cgcttctgca tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca gcaggccgat
    14161 ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgaa
    14221 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
    14281 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac
    14341 cggttgcccg ttctggaaca gatacaccgt cggaatcgcg cgcagaccaa actgggcggc
    14401 aatcatctgc tccgcatcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
    14461 cgcaaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
    14521 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
    14581 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
    14641 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
    14701 cggcagcagg cgcttaagca ccattactgc ccaagtcacc agcgttaccg gatagcgcat
    14761 cttcggcttc tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa
    14821 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
    14881 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
    14941 gtggcgtagc tccatgcgca gcgcgtctga ccatgcctcc agcgcatatt tactggccgc
    15001 gtaagcgcca cgacccggtg tggagattaa tcccatcacc gatgatgtca tcacaatacg
    15061 cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
    15121 aaagttggcg gaaaactgct gttccatctg cgcgcggctg atggtggaaa gggggccata
    15181 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
    15241 ttcgtcggct gcgcgatcaa cactttctgg cgaatccaga tcgatcaaca cgccggtaaa
    15301 tcccatgctg ttcatacgct caacatcatc cggtttccgg caacctgcca gcacatgaaa
    15361 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
    15421 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
    15481 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
    15541 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa
    15601 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
    15661 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
    15721 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
    15781 aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt
    15841 tcagcagagc cggaaggcgc gccagtcctt gttgcgaggt gtcgccgctg atgctggcat
    15901 taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg
    15961 cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg
    16021 cggcacggaa ggttaacagg gccaggaaca ggaagggcaa atgccagcgg aaaacattgt
    16081 tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac
    16141 cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc
    16201 gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt
    16261 gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc
    16321 gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga
    16381 aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc
    16441 gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct
    16501 ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc gacctgatgt
    16561 gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata aaattgccga
    16621 cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga cccacgacct
    16681 gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga
    16741 agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg
    16801 ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg
    16861 gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt
    16921 tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc
    16981 aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc
    17041 aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc ccctggcctg
    17101 aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa
    17161 acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag
    17221 gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg
    17281 gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ttataaattc
    17341 ggcggcaacg agaaccagct cgacggctat gataaatggt tgttacctca gcttaaaccc
    17401 gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc
    17461 tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg
    17521 gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg
    17581 ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg
    17641 acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt gttgatggtg
    17701 ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc gtggctgtgg
    17761 gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg
    17821 ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg
    17881 aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt
    17941 ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt
    18001 gtgctgggct ggatgcagct gaatgtactt cgccgcatga cgctgaaatc gctacctctg
    18061 cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca
    18121 ttttcgctct cctttatgct gctggcgctg ttgctggtat tgcgtggcga tctgctcgac
    18181 cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa catcgccaca
    18241 gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc ggaatcattt
    18301 tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga aggtaatgaa
    18361 gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc cgatcataat
    18421 ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg
    18481 gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag
    18541 ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc
    18601 tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct taccagtttc
    18661 cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc
    18721 ctgttagata ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg
    18781 gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag
    18841 gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg
    18901 gggaaaaaac tgctgcgtac cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttct
    18961 ggcctggtag ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt
    19021 gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcactg
    19081 ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc
    19141 aggcagtttg cggggtgatg acaggtgtta gattgcctga tgcgctacgc ttatcaggcc
    19201 tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata aggcgtttac
    19261 gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc
    19321 ggttttttat atctgtattc tggtgcaacc attctgttct cacgacaatc gccgtttcag
    19381 aataccgccc atcactgcgt cttaacccat tccgccactt ccgcccactc accgcgaaag
    19441 acaacttttt ccgctttttt ctcaagctga tagcgataca tcgggtcgta atattcttta
    19501 agtaacggca ccagccaggc cagatgaccg tcggtgctgt cggtggtgag ttgcgttgtc
    19561 agcgccgtat ccagctgggc agcgagttcg ttatagcgct gtaaccccag ccgacgctta
    19621 atagccgaaa gtccgtgatg caggtattcg caatattcct gccagccctg ttcgtcgccg
    19681 tacgcgtggg taaaatcatg atgcatacgc aagaaatact cttcgttcag gcgctcaaga
    19741 cggatctcaa acggatcttc taccaccgca atcgccgcct gagtcattcg ctcgcgcagg
    19801 cattccggca ggtgattcga accgatcatc cggctttcgt cttccagcac ccacaggcgc
    19861 aaatcctgac gggcgtcggt ttttagcatt tcggcagcca gcaggttttc aaaactcgcc
    19921 tggctaagtt gtggttgtaa ggtgcgacca aacgccgaac cgcgatgacg cgctaaccct
    19981 tccagatcaa caccgttcgg ctgttgctgc actaacagcg ttttaccgct gccggtacaa
    20041 ccgccaatca gcactatcgg tttttgtgcc agttcaatag tcgcctgaat cgcggcttgg
    20101 cgcagtgcct tataaccgcc ttccaccagc ggataatcaa tccccgctgc atacaaccag
    20161 ctttgcacaa tatgtgagcg ctgaccgcca cgggcgcagc agagaatacc ttgcggattt
    20221 tgcaggcacg ctgcccgcca ggcgtccatg cgctgctgac gaatttcacc cgccaccagt
    20281 ttatgcccca gcgccagcgc cgcgtctgag ccttgctgtt tataacaagt gccaacggcg
    20341 gcgcgttcat cgttattcat taacggcagg ttgatagcgg cgggcattgc gccttgctca
    20401 aactcgatag gggcgcgaac atcaataatg ggcgtatcag caatcagcag ggcacgatag
    20461 tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag cttgcgcgct
    20521 attttacgcg ccaacgcgca aggaaacttg atttttaact gcgtgggttg ccgaaaattt
    20581 ctaaaaatcc gctgatttcc ggcctgcgct gggtaaacaa ggtcacaata tcttctaccg
    20641 ctttgccgct gccaaatttg cgccatgcca gactcaatgg cgaaggaggg cgcatcgttg
    20701 ggattacccg gctgaccagt tgttgattat cgatcattga ctggcaaagt gattttggca
    20761 aaaaaccaat gccaacaccc gccagatggg cggcgatttt cgtttccata tcgggaacaa
    20821 taatctcttt ttgccccggc aatcgccagg cgacgcgttt ggttaaggtt cgggcgctgt
    20881 cttcaatatt gaccgccgga aagcgccgca actgcgcttc tgttagcggc tcttcaacgt
    20941 tcgccagcgg atgatccgcc gccatgacaa agcgccattg caccgatcct aagggatcaa
    21001 gactaaaggt atttgccagc gcctcagttc ccgtgacccc gatagccagc gaaaaacctt
    21061 cgtacaatag cgagtcccag acgcccatat agatttgtcg ggagatgtga aactgggtaa
    21121 aggggtaacg ctcattcagc cacgccagca actgggcgac ggcctggggg ttatagagca
    21181 ggttgttgat gacaatattc acctagcgtt ccacgccatc attcacctgt tgcagctcgc
    21241 tgggcatact ttccagccag ctcagccagt ctctggcctg ggaaagtaga tgctcgccag
    21301 ccgctgtcaa cgtcacgctg cgagtcgtgc ggaaaaacag cgccactccg gtattctctt
    21361 ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt tctgccgctt
    21421 ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct ggatcgaaca
    21481 tcttcaggta tcccctttta aatccgcaag ttgcgtgatt ttcttatcct ctgatttatc
    21541 agtattttta catgataacc ctgttcaatt tgtggactaa atctagtttt ggaaaaatat
    21601 tccaactttt gtattgatgt tgttctctta aggttttaga t
//
LOCUS       DAZOXR010000061        21455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZOXR010000061 DAZOXR010000000
VERSION     DAZOXR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.063"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHUNN_001973"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHUNN_001973"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0576357.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHUNN_001974"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHUNN_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0576358.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="ACHUNN_001975"
     CDS             complement(2378..2992)
                     /locus_tag="ACHUNN_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0576359.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHUNN_001976"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHUNN_001976"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0576360.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="ACHUNN_001977"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="ACHUNN_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0576361.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="ACHUNN_001978"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="ACHUNN_001978"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0576362.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHUNN_001979"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHUNN_001979"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0576363.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHUNN_001980"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHUNN_001980"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0576364.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHUNN_001981"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHUNN_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0576365.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHUNN_001982"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHUNN_001982"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0576366.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHUNN_001983"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHUNN_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0576367.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHUNN_001984"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHUNN_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0576368.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHUNN_001985"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHUNN_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0576369.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHUNN_001986"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHUNN_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0576370.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHUNN_001987"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHUNN_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0576371.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHUNN_001988"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHUNN_001988"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0576372.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHUNN_001989"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHUNN_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0576373.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHUNN_001990"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHUNN_001990"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0576374.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="ACHUNN_001991"
     CDS             19125..19937
                     /locus_tag="ACHUNN_001991"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0576375.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21014
                     /gene="tssD"
                     /locus_tag="ACHUNN_001992"
                     /pseudo
     CDS             20331..21014
                     /gene="tssD"
                     /locus_tag="ACHUNN_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            20994..>21455
                     /locus_tag="ACHUNN_001993"
     CDS             20994..>21455
                     /locus_tag="ACHUNN_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576376.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT         5263 a         5740 c         5378 g         5074 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcgtta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagaggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcaggt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaggc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca
    21421 tctgttatca cttaacccat tacaagcccg ctgcc
//
LOCUS       DAZOXR010000062        21005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZOXR010000062 DAZOXR010000000
VERSION     DAZOXR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.064"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /locus_tag="ACHUNN_001994"
     CDS             complement(<1..172)
                     /locus_tag="ACHUNN_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR0576377.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="ACHUNN_001995"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="ACHUNN_001995"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0576378.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="ACHUNN_001996"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="ACHUNN_001996"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0576379.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="ACHUNN_001997"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="ACHUNN_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0576380.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHUNN_001998"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHUNN_001998"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0576381.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHUNN_001999"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHUNN_001999"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0576382.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="ACHUNN_002000"
     CDS             4290..5153
                     /locus_tag="ACHUNN_002000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0576383.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="ACHUNN_002001"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="ACHUNN_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0576384.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="ACHUNN_002002"
     CDS             6491..7108
                     /locus_tag="ACHUNN_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0576385.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHUNN_002003"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHUNN_002003"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0576386.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="ACHUNN_002004"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="ACHUNN_002004"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0576387.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHUNN_002005"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHUNN_002005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0576388.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="ACHUNN_002006"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="ACHUNN_002006"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0576389.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="ACHUNN_002007"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="ACHUNN_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0576390.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="ACHUNN_002008"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="ACHUNN_002008"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0576391.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHUNN_002009"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHUNN_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0576392.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="ACHUNN_002010"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="ACHUNN_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0576393.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="ACHUNN_002011"
     CDS             18074..19783
                     /locus_tag="ACHUNN_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0576394.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHUNN_002012"
     CDS             complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHUNN_002012"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0576395.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5084 a         5713 c         5386 g         4822 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 actctacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgatg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ttgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattctgcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaat ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg tatgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atctcgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtacca cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccaga
//
LOCUS       DAZOXR010000063        20711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZOXR010000063 DAZOXR010000000
VERSION     DAZOXR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.065"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUNN_002013"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUNN_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0576396.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUNN_002014"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUNN_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0576397.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUNN_002015"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUNN_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0576398.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUNN_002016"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUNN_002016"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0576399.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUNN_002017"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUNN_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0576400.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUNN_002018"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUNN_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0576401.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUNN_002019"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUNN_002019"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0576402.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUNN_002020"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUNN_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0576403.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUNN_002021"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUNN_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0576404.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUNN_002022"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUNN_002022"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0576405.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUNN_002023"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUNN_002023"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0576406.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="ACHUNN_002024"
     CDS             complement(14275..16539)
                     /locus_tag="ACHUNN_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFR0576407.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUNN_002025"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUNN_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0576408.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUNN_002026"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUNN_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0576409.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUNN_002027"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUNN_002027"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0576410.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUNN_002028"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUNN_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0576411.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAZOXR010000064        20659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZOXR010000064 DAZOXR010000000
VERSION     DAZOXR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.066"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            2..658
                     /locus_tag="ACHUNN_002029"
     CDS             2..658
                     /locus_tag="ACHUNN_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0576412.1"
                     /translation="MLNQSCKPAHDSPKMYTGIKEFDLSSSWGINNRDDLIQTIYQMT
                     DDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDY
                     VRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQC
                     EDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL
                     "
     gene            655..2109
                     /gene="djlC"
                     /locus_tag="ACHUNN_002030"
     CDS             655..2109
                     /gene="djlC"
                     /locus_tag="ACHUNN_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0576413.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(2169..3839)
                     /gene="hscC"
                     /locus_tag="ACHUNN_002031"
     CDS             complement(2169..3839)
                     /gene="hscC"
                     /locus_tag="ACHUNN_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR0576414.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(3931..4866)
                     /gene="rihA"
                     /locus_tag="ACHUNN_002032"
     CDS             complement(3931..4866)
                     /gene="rihA"
                     /locus_tag="ACHUNN_002032"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0576415.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(4984..5709)
                     /gene="gltL"
                     /locus_tag="ACHUNN_002033"
     CDS             complement(4984..5709)
                     /gene="gltL"
                     /locus_tag="ACHUNN_002033"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0576416.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5709..6383)
                     /gene="gltK"
                     /locus_tag="ACHUNN_002034"
     CDS             complement(5709..6383)
                     /gene="gltK"
                     /locus_tag="ACHUNN_002034"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0576417.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6383..7123)
                     /gene="gltJ"
                     /locus_tag="ACHUNN_002035"
     CDS             complement(6383..7123)
                     /gene="gltJ"
                     /locus_tag="ACHUNN_002035"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0576418.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7293..8201)
                     /gene="gltI"
                     /locus_tag="ACHUNN_002036"
     CDS             complement(7293..8201)
                     /gene="gltI"
                     /locus_tag="ACHUNN_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0576419.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8656..10533
                     /locus_tag="ACHUNN_002037"
     CDS             8656..10533
                     /locus_tag="ACHUNN_002037"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR0576420.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10611..12149)
                     /gene="lnt"
                     /locus_tag="ACHUNN_002038"
     CDS             complement(10611..12149)
                     /gene="lnt"
                     /locus_tag="ACHUNN_002038"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0576421.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12174..13052)
                     /gene="corC"
                     /locus_tag="ACHUNN_002039"
     CDS             complement(12174..13052)
                     /gene="corC"
                     /locus_tag="ACHUNN_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0576422.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13142..13609)
                     /gene="ybeY"
                     /locus_tag="ACHUNN_002040"
     CDS             complement(13142..13609)
                     /gene="ybeY"
                     /locus_tag="ACHUNN_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0576423.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13606..14646)
                     /locus_tag="ACHUNN_002041"
     CDS             complement(13606..14646)
                     /locus_tag="ACHUNN_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0576424.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14799..16223)
                     /gene="miaB"
                     /locus_tag="ACHUNN_002042"
     CDS             complement(14799..16223)
                     /gene="miaB"
                     /locus_tag="ACHUNN_002042"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0576425.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16369..17544
                     /gene="ubiF"
                     /locus_tag="ACHUNN_002043"
     CDS             16369..17544
                     /gene="ubiF"
                     /locus_tag="ACHUNN_002043"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0576426.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17698..17772)
                     /locus_tag="ACHUNN_002044"
     tRNA            complement(17698..17772)
                     /locus_tag="ACHUNN_002044"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17738..17740),aa:Gln,seq:ctg)
     gene            complement(17809..17883)
                     /locus_tag="ACHUNN_002045"
     tRNA            complement(17809..17883)
                     /locus_tag="ACHUNN_002045"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17849..17851),aa:Gln,seq:ctg)
     gene            complement(17932..18008)
                     /locus_tag="ACHUNN_002046"
     tRNA            complement(17932..18008)
                     /locus_tag="ACHUNN_002046"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17972..17974),aa:Met,seq:cat)
     gene            complement(18024..18098)
                     /locus_tag="ACHUNN_002047"
     tRNA            complement(18024..18098)
                     /locus_tag="ACHUNN_002047"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18064..18066),aa:Gln,seq:ttg)
     gene            complement(18133..18207)
                     /locus_tag="ACHUNN_002048"
     tRNA            complement(18133..18207)
                     /locus_tag="ACHUNN_002048"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18173..18175),aa:Gln,seq:ttg)
     gene            complement(18231..18315)
                     /locus_tag="ACHUNN_002049"
     tRNA            complement(18231..18315)
                     /locus_tag="ACHUNN_002049"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18279..18281),aa:Leu,seq:tag)
     gene            complement(18324..18400)
                     /locus_tag="ACHUNN_002050"
     tRNA            complement(18324..18400)
                     /locus_tag="ACHUNN_002050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18364..18366),aa:Met,seq:cat)
     gene            complement(18780..20444)
                     /gene="asnB"
                     /locus_tag="ACHUNN_002051"
     CDS             complement(18780..20444)
                     /gene="asnB"
                     /locus_tag="ACHUNN_002051"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0576427.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(20398..>20659)
                     /locus_tag="ACHUNN_002052"
     CDS             complement(20398..>20659)
                     /locus_tag="ACHUNN_002052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576428.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPDPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT         5019 a         5193 c         5361 g         5086 t
ORIGIN      
        1 aatacttaat cagagttgca aacccgcgca tgactcgcca aaaatgtata ctgggataaa
       61 agaattcgat ctctcatcat cctggggaat aaataatcgt gatgatttaa ttcagactat
      121 ttatcagatg actgatgatg gtcatgccaa tgatttagct ggcttatatc tgacatggca
      181 acgctcttcc cctgaagaga ggaaagcatt aattgctggt ggctcggaga gagggttgat
      241 ttatacccaa ttcgttgccc aaaccgctat gtgctgtgga gaagggggaa tcaaagcctg
      301 ggattatgtt cgaatggggt ttctgtcacg ggtcggtgtg ctcaacaact ggctgacgga
      361 agaagagagt ttatggctgc aatcgcgcgt ctatgcacga gctcaccatt actatcacag
      421 ctggatgcac tatttttctg cctattcact agggcgtctc tactggcaat cttctcagtg
      481 cgaagacaac gcttcgctac gcgaggcgct aaccctgtac aaatacgata gcgccgggag
      541 tcggatgttc gaagagctgg cagcgggaag cgaccgtttc tatgccacgc ttccctggca
      601 accattaact gtccagcctg aatgcccggt aacgcttaag gatgtgagcg acctatgaaa
      661 acatgttggc aaatcctcga aattgaaagc acgacgcaaa tagacattat ccgccaggct
      721 tatcttgctc gcttaccgtt gtgtcatccc gaaaccgatc cgcaggggtt taaagcatta
      781 cgccaggcct atgaagaggc gctgcgactg gcggtaaatc ctgtcgagga agccgatgat
      841 gaagaaaaag atgccaccgc tgaacatgaa atactacgtg cattcaggac attactggat
      901 tcagaaagtg atcgttttca gccctccgcc tggcagaaat ttattcagca attaaatacc
      961 tggaacatgg aggatgtcga tcaattacgc tggccgctgt gtgcaatcgc tatagaagcg
     1021 cgatatcttt cattaaattg tgcttctttg ttggcagagc gtttgaactg gcattcgttt
     1081 aatgacagcg aaggaatgga tgatgaggaa gaaagggagg cttttcttga ggccattcag
     1141 gctggtgatt gtttcgattt ccttagcctt ctggaatatc ccgtcgcgtt acagaaccag
     1201 actgttgaat attacttcgc gctggaacgt tgctgccgtt accatcctga ctatgtcact
     1261 gcgtttttgg cgatggaagg accgtggttc attcctgatg atgcaaagtt acatcgcaaa
     1321 ctgttgcgct ggtacagctc ggtgcaaaca ggtatggcgg aactcattcc tgtagccaaa
     1381 cagtggcaag cggaagaacc agaaagcgaa gatgcccggt attaccagtg tgcacaacgt
     1441 ttgtactgcg gcgaggggga aagcctgctt gccgatctct gcgcgtactg ggaaagttac
     1501 ccatctacac aagctgataa tttgttgttg cagtggagca agcgtcattg cccggattat
     1561 ttcgcgttat tagtgatggt tatcgaagcg cgaagcatgg tagatgcgca aggtaaaccg
     1621 ttgaaatatg ttcctggtga gagcgcccgg acgcggctgt tatgggcgga gattttacat
     1681 agcggaaaat tatcgccgtt aggtcaatcg tttattgagt cgttattctt caagcgcaaa
     1741 gcatgggcgt ggtggaaatc gagagtcggt agcgagacag agcaagattc accgttactg
     1801 gatttgtatc gggtagcgga acaggttgtg ctggaagcgt tcccgaaaca agagatgctg
     1861 gcccgtctta atacaaggct ggaaggtgga gatactcatc cattagaggc cattatcacc
     1921 cagatgctgt tgacgaaagt gaaactcgag ccggaggatg aagatgtcga tgagccaaca
     1981 cctgaaaacc atgaagaaaa aaatgatgag gatgaaaaac cacagagcat taccagtatt
     2041 atcaaaatca gcttaacggt gctgatgata ggttatgcgc tcggaaaaat cgcgatgttg
     2101 tttagctgag gatgtgtttt ttgcgccgat gccggatgca gcgtttatgc cgcatccggc
     2161 agccgtgttt aagggatctc aatagccaaa taatcgctcg cccgtcgacg aacttcatcg
     2221 acgcgggcca tatcgtttga ctgcaacgcc gcatcaaaat cggtgatcac ccggccaatc
     2281 tcttctcgct cgtcacccag cgcccgcgcc cacaactctt ccagtttggc cttaaaggtg
     2341 cggttgatta gcatatcgcg cggataaatt ttcaatgcgg ataaccgcgt ccgactctct
     2401 tcaatctgct gcgcgctcaa tgttaccggg ctatgattaa tgactctgga cttaacgctg
     2461 ccatcttcca gaagcacgtc aacttcaagc aagccgttga tatcataact aaagcgaata
     2521 tcaatcgact gataagcccc ggttttcttc aacggcacat cgaaggattc aaccagaatg
     2581 ttatttttaa ctttgtggtt ttctccctga tagacgttaa ccgtaataga atcctgttcc
     2641 gggtgcatgg tcgagtaagt ttctacacgc gacacgggta ctgtggtgtt tcgttcaata
     2701 attggcgaga aaatgccgga aacgccctgg cggttaactt caacgcccaa cgagtaaggg
     2761 caaatatcag tgaggatcac ctcttcaata tcttcactgc gtaagcggca ggcggcctgg
     2821 attgctgcgc ccagcgcgac aatggtgctc gggtcgtaac tttgatacgg taatttgcca
     2881 aacagacgca cggcgattcg ctgcaccagc ggcatttgtg acgcgccgcc aaccagcacc
     2941 agactgtcga tttgactcgg cttcagacgt gcatcgcgca acgcctgttc aatcggcact
     3001 cgcaagcgat tgagcagcgg caaccacaaa tcttccagtt cgttctcgta aaattcgcac
     3061 tcccgcgttt catcctggta ctgccagcga atgtgcaatg gcgattgatt gctacatttt
     3121 gccgcttcca cacaggcgta caaggctgcc agttcactct cgttaagcgt ggtcctggcg
     3181 acatccgcgc gctttaaaac gtcatcgacc aacatatggg taaaatcttc gccaccgagg
     3241 aagttatcgc cagcggaggc gtgaacttca atcaccggcg tggcgtattc gagcaccgtg
     3301 acgtcaaacg tgccgccacc gagatcaaaa accagcgagc gggtattttg ttgcgtatgc
     3361 aggccatatg ccatcgcagc tgctgtgggt tcattaatta agcgtaccgc atttaacccg
     3421 gctaactccg ctgctaaacg ggtatgcttg cgttgttcat cgctgaaata agccggaacg
     3481 gagatcacca catctttaat cggacgttgc agaaattctt cggcatcttc ttttaatgag
     3541 cgtaatacca aagaggacag ttctggcgcg ttaaatgtgt cgctgcctaa ccgccagttg
     3601 gtattactgc ccattgcacg tttaaataac gctgccgttt tatccggatg cgaagtacgc
     3661 cgtgatacag ccggttttcc gactaaaata tgattatttt catccatgct aattatggat
     3721 ggtgttaaat attcaccgaa cttatttgga attaattgcg cggcaccgtc tttccagacg
     3781 gcaattaaac tattggtagt gccgagatcg ataccaatgg cgagttctgc attatccatt
     3841 gcatttatat cttgaaagaa aaagagataa acagataaac agattaagac cacccaggta
     3901 acgcgtggtg atcttaatca atgacgtgtg ttaagcgtaa aatttcagac gatcagccag
     3961 taaatcaaca aagccctgac gatcaacatc gaccattaca gtggcattcg gtttattgcc
     4021 agtcagataa taataatcaa caaccgtcat accctggcta tatttcccct gtgtttccac
     4081 gccaacccag cgctcaacag tggtaaacaa ctcgggtttc aacagccagg cgatggtgca
     4141 tgggtcatgc agcggtgcac cgacaaagcc ccatttttcg tctttatgat attcgaggaa
     4201 gaaatccagc agttcggcaa caatggttga aacagggttg ccaatcgcgc ggaaacgctc
     4261 ggtgtcttca acgtggattt gtgctttgtg agtaacatcc agaccggcca tcaccactgg
     4321 gatccctgac tggaagacaa tttctgccgc ttccgggtca acgtaaatat taaattcagc
     4381 cgcaggcgtc cagttaccaa gccccattgc gccgcccata atcacgatac gggcaatttt
     4441 gctatgcagt tccggatggc tattgagcag caaggcaacg ttagtttgcg gtccggtaga
     4501 cacaatcgtg acaggttccg cactttcgcg cagcgttttc gccatcagct ctaccgccgt
     4561 acagttttgc ggtgcgaacg tcggttccgg taatgccggg ccatccagac cgctttcgcc
     4621 gtgcacattg tccgcgataa tcaactcacg cattaacggt tttaccgcgc cgcctgctac
     4681 cggaatatcg gtgcgattaa gcaaggtcag catacgcaga acattgcgta aggttttttc
     4741 tggtgtctgg tttccggcgg aagacgtaat tgctttgaca tcaagctctg gtgaggcgag
     4801 ggcgagaact attgcgatag cgtcgtcatg acctgggtcg caatctaaca gaattggcag
     4861 tgccattgtt gctccttgtt gtgtgcttct ttgcgacaag ggtaacgcca ggatgtaaca
     4921 gatacgaggg gcgaaacgat aaagcgtgag atggcgcgca cttgggtatg cgcgccagag
     4981 tgattaatgc aggattttcg cgaggaagtc tttcgcgcgg tccgatttcg gatcatcgaa
     5041 gaaagcgtct ttcggcgagt cttcgacaat tttaccctcg tccataaaga tcacccgatt
     5101 cgccactttg cgggcaaagc ccatttcgtg ggtcaccacc atcatggtca ttccttcgtt
     5161 cgccagttcc accatcacgt ccagcacttc gttgatcatc tccggatcca gcgccgatgt
     5221 cggttcgtcg aacagcatcg caataggatc catacacaat gcgcgagcga ttgccacacg
     5281 ctgctgctga ccgccggaaa gctgcgccgg aaatttattg gcatgtgcgg aaagtcccac
     5341 gcgctccagc aatttcaatg ctttttcacg agcgggcgct ttatcgcgtt taagcacttt
     5401 cacctgcgcc agggtcaggt tttcgataat cgacagatga gggaacagct cgaaatgctg
     5461 gaataccatc ccgacgcggg aacgcagctt tgccagatcg gttttcttgt cattaaccac
     5521 gataccatcg acggtgattt caccttgctg cactggttcg aggccgttga cggttttaat
     5581 cagcgttgat ttgccggaac cagacgggcc gcaaaccacc accacttcgc cttttttcac
     5641 ttcggttgag cagtcggtca gcacctgaaa gtgaccatac cattttgaaa catttttcag
     5701 ggtaatcatt atgctgtcct tcttttcaag tagctgacca acaacgacgc gctaagacta
     5761 ataacgaaat aaacaaatcc ggcaaacagg atcatctcaa cctgcgtacc atcacgctca
     5821 ccaatggttg aggcggtacg gaagaaatcg gccagagata acacatacac cagtgaggta
     5881 tcctggaaca gtacgatgcc ctgagtgagc agaagcggca ccatcgcgcg gaacgcctgc
     5941 ggcagaataa tcagtttcat cgactgccag tgagtcattc ccaacgccag cgcggcgctc
     6001 gactgaccac gagaaatact ttgaatacca gcacggataa tctctgaata gtaggccgct
     6061 tcaaacatcg aaaacgccac catcgccgaa attaaacgga tatcattttt tggtgataat
     6121 cccagcacgt tttgcagaaa acctggcacg atcaggtaaa accacagcaa aaccataact
     6181 aaaggaatcg agcggaatac gttaacgtag gctttggcaa accacgctac gggcgcaaag
     6241 ctggacaagc gcatcaccgc cagcatggtg ccccacaaaa taccaatcac gactgccgtg
     6301 acggtgattt tcagcgtgat caccagcccg tcgagcagat atggcaggga agggacaatg
     6361 gaactccagt caaactcgta cattatttgc cccccatgtt gccaggcagg cgaactttac
     6421 gttcaaccag cgtcattacc agcatgataa ccgcgttaat caacacatac gccagcgtaa
     6481 tagcggtaaa cgactcccag gcgtgggctg agtaatcaag caatttaccc gcctgcgccg
     6541 ccatatccac cagaccaata gtcgaggcga tggcggagtt tttcaccagg ttcatcatct
     6601 cggaagtcat cggcgggacg ataacgcgat aagcattagg cagcagtacg tatcgataag
     6661 cctgcggtag cgtcaggccc atcgccagcg cggcattttt ttgcccacgc ggcaacgact
     6721 gaatggcggc gcgtacctgt tcgcaaacac gggcggcggt aaacagcccc aggcagagca
     6781 tggatgaaag gaaaaactga atattgggat ccagctcggc cttaaaccac atgccgattt
     6841 tctccggcaa cagctccggg atcaccagat accaggtaaa gaattgcaca atcaacggca
     6901 cgttgcggaa cagttcgaca tacaacgtac ccagaccgga gagaaaacgg tttggaacgg
     6961 tacgtaaaat gccgaaaaat gaaccgacga ggaaagcgat aatccaggcg cagatcgaca
     7021 aagcgatcgt cacctgaaaa ccactccaga tccaaccaag ataggtggtg ttgccgaacg
     7081 gggcttgttg taaaaaaata ccccagttcc agtctataga cataaatcta ctccagaaaa
     7141 aagagggtag cagcgttaac tgctaccctc gaagattgtt acccagcgta ttgcggtttt
     7201 caggcccgat ggggaacgac catcaggcgt atagtctgtc cgtgctacgt aacaatcgag
     7261 agggctggaa tttccgcccc tggttcttgt aattagttca gtgccttgtc attcggttct
     7321 ttgaacagtg ctttcatttc gtctgacagt tcgaaattca tgttcaggtt tttcggcgga
     7381 atgggatttt tgaaccattt atcaaaccat ttttccgctt caccggaggt ctgcacctgc
     7441 gcggtggtgt catccatcag ctttttgaac tgcggatcgt ctttacgcaa catgcaaccg
     7501 taggcttcct gagactgcgg cttgccgacg atttcccagt tgtctggttt cttcgctttc
     7561 gcacgttcac cggccagcag ggcatcatcc atcataaagg caacggcacg accgctttcc
     7621 agggtgcgga aagagtcact gtgatctttg gcgctgatga tgcgcatatt cattttttgc
     7681 tcttcattca gtttgttgag caaaacttca gaggtagtgc cggaagtgac gactacggct
     7741 ttgtctttca ggtcggcaaa atctttgata tcgccgccct ttttggtcag caggcgcgta
     7801 ccgaccacga aaatagtatc agagaaagcc gcctgttttt ggcgttcgac gttgttggtg
     7861 gtagaaccac attcaaaatc gaaagtgccg ttttgcagca gtggaatacg gttttgtgag
     7921 gtaatcggaa tcagttttac ctgcaagtcc ggtttgttga gtttcttttt cactgcttca
     7981 acaatggcgt tggagtaatc ctgcgagtaa cccaccactt tttgctgatt gtcgtaataa
     8041 gagaaaggta ctgaagattc acggtgaccg acgacaatca caccgttttt ggcgattttg
     8101 tccagcgtac tgcctgctgc cggggcggcg tcatctgcct gtgccagtcc tgcggaaagc
     8161 gccagggcga ggattgctgt ggcaggttta cgtaattgca tatccaactc ctttatcttc
     8221 tgcgttaaga acgcatggat acccgttgtg agtgtttgtg ttgttatcgt ctgcaacttt
     8281 attgtgcagt gttgtgcctg ttaggtaaca tttagtttgg ctaaatgtaa agatattgct
     8341 gttttattgt ttgtttttgc gagatgcgcc gcaccattcc gaagcaaaat tcttaaaccg
     8401 cacctttttg gtgctaccgc tgatttgtgg tggtgcaacc tggttgcgtc gcggctgatt
     8461 tcatcgctta cacagtaaat aaagcaactt acatgccaaa aaatacttag gggcgaatta
     8521 attacgattg taattttagg ctgaaaaagt catatttcgg acagaattat cttttcggca
     8581 tgggacgttt gccgggatga taactgcaca cgggaaaaat gctttttacc tgtcttttca
     8641 aggaagaaac accttatgtc tgcatcttcg gttaagccgt taaatgttca attacccgca
     8701 ataaccctta tcctttttgc actctgtatt gggatatttt gttatctcgc acaatggatg
     8761 agttatgaag aggtcgatca atccgcactt atccatctcg gcgcaaacgt tgcgcctctt
     8821 actttgtcgg gtgaaccctg gcgcttattg agcagtatct ttctgcacag tagtgtttct
     8881 catctgctga tgaatatgtt tgcattcctg gtcgtgggag gcgtggcgga acagattctg
     8941 gggaaatggc gactcctgat tacctggtta ttctccggcg tctttggtgg gctcatcagc
     9001 gcctgttatg cgttacgcga aagtgagcag atagtcatca gcgttggggc atccggggcg
     9061 attctgggaa tagctggtgc agcgatagca acacagtttg cttcaggtac gggcacatac
     9121 cataaaaacc agcggcgagt atttcctctg ttgggtatgg tggcgctgac actgttgtac
     9181 ggtgcccggc aaacaggaat agataacgct tgccacattg gcggcctgat tgcgggtggc
     9241 gcgttgggtt ggctgagcgc gcgtttatct gggcaaaacc gactcgttac ggaaggcggg
     9301 attattgttg cgggcagtct tcttctgacc ggggctatct ggcttgcgca gcagcagatg
     9361 gatgagtcag ttttacaggt caggcaaagc ctgcgtgaag cgttttatcc acaggagatt
     9421 gaacaagagc gacggcaaaa aaagcagcag ttagcggagg aacgcaacgc cctcagggaa
     9481 acattatccg ctccggtaag tcgtgaacag gccagtggtg atttgctcgc tgagattgcc
     9541 gatatccatg atatggcgat cagtcgggat ggtaatatgt tgtatgccgc aattgaaaac
     9601 acgaacagca ttgttgtttt cgacctcgga caaaagaaaa tcctgcatac ctttacagcg
     9661 cccatagcga aagaaaagtc agtcaaacat tgtggtggct gtaaagatca gggcgtcaga
     9721 tcgctggcat taagcccgga tgaaaagttg atttatgcga cttcatttga agcgaatgcg
     9781 ttatcggtca ttaacgtggc gacaggggag attattcagt cgattaccac cggtgcacat
     9841 cctgacagcc ttatcctctc gcgtgatggc acaaaagcct gggtgatgaa tcgcaccagt
     9901 aatagtgtgc cagcgattga tctggtgact tatcagcatg tggcggatat cccgctggag
     9961 aaatacgacg ggacggggac gagcggtaaa ccaggcgcct gggttatggc actttccccg
    10021 gatgaaagaa cattactggt tccgggagca ggcagaggta acatcgtgcg gatcaatacc
    10081 atcacgcatc agaaagaaga ctttcccgca ggtgatgcgc gtggaacgat atcggcgatg
    10141 cgttttcgac ctgaaaacgg cgaggttatt tttgccgata gtcaggggat ttcacgtata
    10201 agcgtagggg atcaacaagc cagcattatg acgcaatggt gtagcaggag cgtttattcc
    10261 gttgagggta ttagcccgga cggtcaatat ttagcgttgg tgtcatatgg cctgcaaggt
    10321 tatgtcatcc tgctcaatat taatgccggg cagattattg gcgtttatcc tgccagctac
    10381 gttaatcacc ttcgtttttc agcggatggt agaaaaatat ttgtcatggc gaagaacggg
    10441 ttgatccaaa tggacaggac gcgctcgctt gatccgcagg caattattcg tcatccccaa
    10501 tatggcaatg tggcttgtat ccctgaaccg taagaaaaag gggcatgata accatgcccc
    10561 aaagctcaat gagaaagtac gttttgctca tgcaggatac atcaggcaca tcaacgttta
    10621 cgtgctcgca gactcatcaa cacagcggca aaaccaaaca gcgccgtcag cacccacagt
    10681 ggccagttgc cggtacgtgc atacggtgtg agtccggtgg tcggtgtcac attagtggtc
    10741 aacacctcgc gggtgaactg cgggatcatc gcctgaacct ccccctgcgg gccaatcacc
    10801 gccgtaatgc cgttgttggt gctgcgcaac agtgggcgcg ccagctccag cgcacgcatt
    10861 cgcgccatct ggaagtgctg ccatggacca atagacttac caaaccacgc atcgttggag
    10921 atagtcagca gatagtcggt atccgggcgg aagttatcgc gcacttgctc gccgagaatg
    10981 atctcgtagc aaatagccgc agtaagctca ataccattta ccgacagcgg cggctggata
    11041 tatggcccac ggctgaacga cgacatcggc agatcaaaga acggtgctaa cggacgcaga
    11101 atcgactcca gcgggacaaa ctcgccaaac ggcactagat ggtttttgtt atagcgatcg
    11161 gctgattcgt agctgtatgg tgcaccttta cccagtgtga tgatggtgtt gtaggtatcg
    11221 tagcggttct gcttattgag acgcgcgtcg acaatcccgg ttaccagcga gctaccttta
    11281 tcgcgcaact caccgtccag tgctttgagg aacggttgct gattaatttc cagatcggtt
    11341 atcgccgact ccggccagat aatcaacgat gatttgccca tcagcggagc cgttgcgttg
    11401 tagtaaatct tcagcgtatt aagaagctga tctccatccc atttcagtga ttgcggaata
    11461 tcgccctgaa ccatcgaaac ctgaatggtt ttctccggtt gcggggtaaa ccattggatg
    11521 taacgcagcg ggaagggtag ggcaaacagc acgacggcca ccaccagcgg acgccagttg
    11581 cgtttgacca acgccagtgc cagcaggcca ctaaccatca tcagcaggaa gttaatggct
    11641 tccacgccca ttatcggtgc cagccctttt aacgggccat caatctggct atagccgaac
    11701 tgtaaccacg ggaagcctgt cagtacccaa ccgcgcagaa actcggtcac ttgccagaga
    11761 gcaggggcgg caatcgccac gcgcagccag gtggttttcg gccacagacg cgacagcacg
    11821 ccagcaaaca gtccggtata cagcgacaaa tacgccgcca gcagcaccac caggaagatg
    11881 ttaaccgggc caggcattcc gccaaaggtc gcgatgctga catagaccca gttaataccg
    11941 ctgccaaaga ggccaaatcc ccagcaaaag ccaatagcgg cagactggag tggacggcgg
    12001 ttaaaggtca acgcctgaag ccccatcaac gaaataatcg ccgcaggcca gacgtcgtaa
    12061 ggagagaagg ccagcgttcc gcaggcaccg aataataacg ccagcagcag gcgaatgcgc
    12121 tggcgttcaa ttaatgaggc aaaagccatg tagttatcta tccagtttcg gttttattca
    12181 tccagcttcg gctggggtga gtcatccggg attttgacat gaacctgaat aatacgccga
    12241 ctgtcggcca tcgccacttt gaactggtaa ccgtcgatgt cgatagtttc gccacgcgcc
    12301 ggaagatgcc caaatgcctg catcaccaga ccaccgatag tgtcaacctc ttcatcgcta
    12361 aagtgggtgc cgaacgcttc gttgaagtct tcaatggaag ccagtgcacg cacggtccag
    12421 gtatgacgac tcagctgacg gaagtcgata tcatcttctt cgtcatactc gtcttcaatc
    12481 tcaccaacaa tcagttccag gatgtcttca atggtcacca gaccggaaac cccaccgaat
    12541 tcgtcaatga cgatcgccat gtggtaacgc tgagagcgaa actctttcag catccggtct
    12601 acgcgcttac tttcaggaac gacaaccgcc tgacgtaaca ctttgtccat gctgaaggct
    12661 tcagcatcgc tgcgcataaa cggcagcaag tctttcgcca tcagaatccc ttcaatgtga
    12721 tctttgtctt cgctaatcac cgggaaacgt gagtgggcgg actcgatgat gacatcaagg
    12781 cattcgtcca gcgtctggtt gcgtttcagg gtaatcatct gggagcgggg gatcatgatg
    12841 tcgcgaacgc gttggtctgc gatgtccatc accccttcga gcatatcgcg cgtatcttcg
    12901 tcgataaggt cgttctgccc ggaatcacgg atcagcgcca gcagttcgtc acggtttttc
    12961 ggttcaccgt ggaaaagttg gctgagtaac agggagaaaa atcccttctt gttgcttatc
    13021 gtgtcactac tgtgtgaatt gtcgtcgctc atggcgtcgt atgggttctc atgttagtta
    13081 atcaaaacgt cgtcgttaat caccaacggc ggggacgtct gccagtcaaa tgcctggcaa
    13141 attattcttt ctcggcaatg tacggatcct catagcccag agcaagcata atctctgttt
    13201 cgagggcttc catttcttct gcttcgtcat cttcgatgtg atcgtaacct aacaaatgca
    13261 gactgccgtg caccaccata tgcgcccagt gcgcctccag tggtttgcct tgctcctgag
    13321 cttccttctc aaccacctga cggcagataa ccagatcgcc cagtagcgac atttccatgc
    13381 caggcggcac ttcaaacggg aaggagagca cgttggtcgg cttatcctta ccgcgatagg
    13441 tcagattcag actgtggctt tcggcggtat cgaccacgcg aatagtcact tccgattctt
    13501 cctgaaactg cgggatcacc gcattcagcc atgtttgaaa ctggctctct tccggtaacc
    13561 cggaattatc ttcacatgcc agttgtaaat cgaggatcac ctgactcatt tttgttcctg
    13621 ttcttcgcgc ttgcgttctg ccgccagcgc cgcttttcgt ttttgttcgg cttcttccca
    13681 ggcttcatag gcgttaacga tacgcgccac cacagggtga cgaaccacgt cttcgctgtg
    13741 gaagaagtta aagctgatct cttcgacatc ggccagcact tcgatggcgt gacgtaagcc
    13801 tgatttagta ttacgcggca ggtcgatctg tgtgacgtcg ccggtgataa ccgcttttga
    13861 gttaaaaccg atacgggtca ggaacatctt catctgttcg atggtggtgt tctggctctc
    13921 atcgagaatg ataaacgcgt cgttcagcgt acgaccacgc atataggcca gcggcgcgac
    13981 ttcaataacg ttgcgctcaa tcagtttctc aactttctca aagcccagca tttcaaacag
    14041 cgcgtcgtac agtgggcgca gatacgggtc tactttctgg cttaaatcgc caggcaggaa
    14101 gcccagtttc tcaccggctt cgaccgccgg acgagtcagc agaatacggc gaatctcctg
    14161 acgctccagg gcatcaactg ccgcagccac tgccaggtag gttttacccg tacccgccgg
    14221 gccaacgccg aaggtaatgt catggtcgag aatattggcg atgtactgcg cctggtttgg
    14281 cgtgcgcggc ttaattacgc cgcgtttggt tttgatattg accgctttgc cgtactccgg
    14341 cacgctctcc gcactttgct ccagtactcg cgcttcttta atcgcaaggt ggatctgttc
    14401 cggttcgata tcctgaatct gaccgcgcat cggggctgta tcgacataca ggctacgcag
    14461 aatgtctgcc gcagcggtga cgcaaatcgg acggccggtc agtttaaagt ggttgtcgcg
    14521 gcgattgatc tcgatgccga gacggcgttc gagctgcttg atgttgtcat caaacgggcc
    14581 gcacaggctc aacagacgcg cgttgtctgc tggctccagg gtgatttcgc gagtgtctat
    14641 gttcaaaccg ttcctcttat ctgtatgccg ccggaagctg aacattcacc ggcctataag
    14701 gaaattattc acgccacagg aaaaaggcgc aagcattgcg ataaagatgg ggataaagag
    14761 agaaaaaaca aggcccaccg gaacggcggg cctgagaatt acggctgata ataacccacg
    14821 ccaaggtcgt tttctttgcg ggtacgggca atcaccgatt ccggtgtttc tgccacgcgc
    14881 agacccattt catcttcagt acgcaccact ttaccgcgca gagagttcgg gtagacgtcg
    14941 gtaatttcta catcgacgaa tttaccgatc atatccggtg tgccctcgaa gttgaccacg
    15001 cggttatttt cggtacgccc ggaaagctcc atgatgctct tacgcgaagt cccttctacc
    15061 agaatacgct gggtggtgcc gagcatccgg cggctccatg ccatcgcttg ctgattgata
    15121 cgttcttgca gaatatacag acgttgcttc ttctcttctt ccggaacatc atcaaccata
    15181 tcggcggctg gtgtacccgg acgtgcagag aagataaagc tgtagctcat gtcgaaattg
    15241 acgtcggcaa tcagcttcat cgttttctcg aagtcttcgg tggtttcgcc agggaagcca
    15301 acaatgaagt cagaactgat ctgaatatcc ggacgcgccg cacgcagttt acggatgatc
    15361 gctttgtact ccagcgccgt gtgagtacgg cccatcaggt tcagaatgcg atcggaaccg
    15421 ctttgtactg gcagatgcag gaagctcacc agctccggcg tgtcgcgata cacttcgatg
    15481 atatcgtcgg tgaattcgat cggatggctg gtggtaaagc gaatacgatc gatcccgtcg
    15541 atcgcagcaa ccagacgcag cagatcggca aacgatccgg tggagccgtc gtagttttca
    15601 ccacgccagg cgttcacgtt ctgaccgagc aggttgactt cacgcacgcc ctgagccgca
    15661 agctgggcaa tctcaaacag aatatcgtca gacggacggc ttacctcttc accacgggtg
    15721 taaggcacca cgcagtaggt gcaatattta ttgcagcctt ccatgatgga gacaaacgcg
    15781 gtcggccctt cggcgcgcgg ttccggtaga cggtcaaact tctcgatttc cgggaagctg
    15841 atatctacaa ccgggctgcg gtcgccgcgc acggagttga tcatctccgg cagacggtgc
    15901 agcgtttgcg gcccaaaaat aatatcgaca tagtgggcgc gctggcgaat gtgctcgcct
    15961 tcttgcgatg ccacgcagcc accgacgccg ataatcaggt ctggattctt ctcttttaac
    16021 agtttccagc gacccaactg atggaagact ttttcctgag ccttctcgcg gattgagcag
    16081 gtgttcagca gcagcacatc cgcttcttcc gccacgtcgg tcagttgata gccgtgggtg
    16141 gcatccagca gatcggccat cttcgatgaa tcgtactcgt tcatctgaca gccccaggtt
    16201 ttaatatgga gttttttggt catcgacttg ctcttgcgaa atagtagcca ggaatgcagg
    16261 gcgtatagtg taatgctttg ctgccgttgt gaccagtatg agcgttatca gcccttgggg
    16321 gtaaaaatcc tgtaaactta aagcatattg ctaacaggat gattgaccat gacaaatcaa
    16381 ccaacggaaa ttgccattgt cggcggagga atggtcggcg gcgcgctggc gctggggctg
    16441 gcacagcacg gattttcggt aacggtgatc gagcacgcag aacctgcacc gtttgtcgct
    16501 gatagccaac cggacgtgcg gatctcggcg atcagcgccg catcggtatc attgcttaaa
    16561 gggttagggg tctgggatgc agtacaggct atgcgttgcc atccttaccg cagactggaa
    16621 acgtgggagt gggaaacggc gcatgtggtg tttgacgccg ctgaacttaa gctaccgttg
    16681 cttggctata tggtggaaaa caccgtcctg caacaggcgc tgtggcaggc gctggaagcg
    16741 catccgaaag taacgttacg tgtgccaggc tcgctgattg cattacatcg agataatgat
    16801 cttcaggagc tggaactgaa gggcggtgaa acgatccgcg cgaaactggt gattggtgcc
    16861 gacggcgcaa attctcaggt gcggcagatg gcgggaattg gcgttcatgc ctggcagtat
    16921 gcgcagtcgt gcatgttgat tagcgtacag tgtgagaacg atcccggcga cagcacctgg
    16981 cagcaattta ccccggacgg accgcgtgcg tttctgccgt tgtttgataa ctgggcatcg
    17041 ctggtgtggt atgactctcc ggcgcgtatt cgccagttgc agaatatgaa tatggcgcaa
    17101 ttccaggcgg aaatcgcgaa gcatttcccg tcgcgtctgg gttacgttac accgcttgcc
    17161 gctggtgcgt ttccgctgac gcgccgacat gcgttgcagt atgtgcagcc ggggcttgcg
    17221 ctggtgggcg atgccgcgca taccatccat ccgctggcgg ggcagggggt gaatcttggt
    17281 tatcgtgatg tcgatgccct gattgatgtt ctggtgaatg cccgcagcta cggtgaagcg
    17341 tgggccagtt atcctgtcct caagcgttac cagatgcggc gcatggcgga taacttcatt
    17401 atgcaaagtg gtatggatct gttttatgcc ggattcagta ataatctgcc gccgctgcgt
    17461 tttgtgcgta atctcgggtt aatggcggca gagcgtgctg gcgtgttgaa acgtcaggcg
    17521 ctgaaatatg cgttagggtt gtagccttac aacattgccg gatgcgtgcc aaccgtaggt
    17581 cggataagac gcggcagcgt cgcatccgac attgaaggat aagacgtgtc aacatcgcat
    17641 tcgacattga atgaacgcag aaaagcaaaa agctcgccga agcgagcttt tttaatgtgg
    17701 ctggggtacg aggattcgaa cctcggaatg ccggaatcag aatccggtgc cttaccgctt
    17761 ggcgataccc caactggtgc acttacgatg taagcgtctt gaataaattg gctggggtac
    17821 gaggattcga acctcggaat gccggaatca gaatccggtg ccttaccgct tggcgatacc
    17881 ccaacaaatt ggtttttgaa tttgccgaac gtattcgaca cattcagaat ttggtggcta
    17941 cgacgggatt cgaacctgtg accccatcat tatgagtgat gtgctctaac caactgagct
    18001 acgtagccag attgtttctt cgatggctgg ggtacctgga ttcgaaccag ggaatgccgg
    18061 tatcaaaaac cggtgcctta ccgcttggcg ataccccaat aaccggtcgg tgaaccgctt
    18121 actcgaagaa gatggctggg gtacctggat tcgaaccagg gaatgccggt atcaaaaacc
    18181 ggtgccttac cgcttggcga taccccatcc gtacaacgct ttctggtgaa tggtgcggga
    18241 ggcgagactt gaactcgcac accttgcggc gccagaacct aaatctggtg cgtctaccaa
    18301 tttcgccact cccgcaaaaa agatggtggc tacgacggga ttcgaacctg tgaccccatc
    18361 attatgagtg atgtgctcta accaactgag ctacgtagcc atcttttttt cgcgatacct
    18421 tatcggcgtt gcggggcgca ttatgcgtat agagccttgc agcgtcaacc tctttttcaa
    18481 ggaaaattgc tcgaaagtga ctgtttggtt aggttgcgaa cagcgtggcg ctatattcgt
    18541 caattattgt ttactttgtg ttttttcccc accctacagc cattcttttg tcatacagga
    18601 tgaaattcgg aattcaacaa tggtggtggg aaattaatct atgaaatact ggcctacagt
    18661 gatgagttgt caaacagtga tgtggcaaac ccggaacatt tccttactgc atatcagaat
    18721 caacaagcac ctcaataact gaaacagccc cggatttcac cggggctgtt tcgcatttct
    18781 tacttatacg ccgactggtg aacgccaacc gcacgaccag acggatcgtc cattttcttg
    18841 aacgcctcat cccactcaat cgctttagcg gaagaacacg cgacggacgg gccgcccggc
    18901 acgcactcag cggcgctcgg aagcgggaac agttcttcaa agatctcccg gtacagatac
    18961 gcttcttttg aggttggcgt gttgtacggg aagcggaagc gggcagtttc cagttgctga
    19021 tcagaaacct gctgcgccgc cacttctttc agggtgtcga tccaactgta accgacgcca
    19081 tcggagaact gctctttctg ccgccaggcc acgcttgcgg gcaggtatga ctcaaaacat
    19141 tcacgcagga tgtgtttttc cattttgccg ttaccgcaca ttttatcctg cgggttaatg
    19201 cgcatcgcca catcaaggaa ttttttgtcg aggaacggaa cgcgtgcttc cacgccccag
    19261 gctgacatcg ctttgttggc gcgcgcgcag tcatacatat gcagggccag cagtttacgc
    19321 accgtctctt catgcagttc tttagcgttg ggcgctttat ggaagtaaag ataaccgcca
    19381 aacacttcat cagaaccttc accggacagc accattttaa tgcccatcgc cttgatctta
    19441 cgcgacatta aatacatcgg tgttgaagcg cgaatagtgg tcacatcata agtttcgatg
    19501 tggtaaatca cgtcgcggat ggcatccaga ccttcctgta cagtgaagtg aatttcgtga
    19561 tgcaccgtgc ccagatggtt tgccacttcc tgggctgctt taagatccgg tgaacccggc
    19621 agacctacag caaaggagtg taactggggc caccaggctt cagagcgttc ctgatcttcc
    19681 acgcgacggg ctgcgtattt cttggtgata gcggaaataa ttgaggaatc cagaccacca
    19741 gaaagcagca caccgtaagg cacatcagac atcagatggc ttttaacgga atcttccaga
    19801 gcctgacgca gctcgttttt gtcggtcacg ttatctttca ctgcatcaaa gtcgaaccag
    19861 tcgcgatgat aataagaacg gatttcgccg tcctggctcc acaaatagct ccccgccggg
    19921 aactctttaa tcgtgcggca aactggcacc agcgatttca tttctgaggc cacatacagc
    19981 tgaccgtgtt cgtcataccc catatacagt gggatgatcc ccagatggtc gcgaccaatc
    20041 aggtaggcat ctttttcgct gtcgtacagg gcaaaggcaa acatgccctg caagtcgtcg
    20101 agaaattccg gccctttttc ctgatacagc gcgaggatca cttcacagtc agaccctgtc
    20161 tggaactgat aacgatcgcc atattcagcg cgcaatgcct ggtggttgta gatttcaccg
    20221 tttaccgcca gtacgtgggt tttttgttgg ttgtagagag gttgcgcccc cgcgttaacg
    20281 tcaacaattg acagacgttc gtgagcgaga atggcgttat cgctggcata aataccggac
    20341 cagtccgggc cacgatgacg catcaggcgt gacagctcga gtgctttctt acgctgctca
    20401 actgcgtctg ttttgatatc gaatacgcca aaaattgaac acataacctt ctccgttaac
    20461 ctggtatttg ttgcttgttg tgtttgcttg tttaaaaaaa tgccgcaaag cagcactgtg
    20521 cgcaagcgat ttggcggtga aaaaataaaa aacgtaatgg tgattgtcga ttggtgaaaa
    20581 agggtctggt gtgaggatgt atttattgat gaatcgataa tttttagcgg gttttattga
    20641 atggttatat tttacgggg
//
LOCUS       DAZOXR010000065        20400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZOXR010000065 DAZOXR010000000
VERSION     DAZOXR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.067"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHUNN_002053"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHUNN_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0576429.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHUNN_002054"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHUNN_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHUNN_002055"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHUNN_002055"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR0576430.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHUNN_002056"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHUNN_002056"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0576431.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHUNN_002057"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHUNN_002057"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0576432.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHUNN_002058"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHUNN_002058"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0576433.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHUNN_002059"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHUNN_002059"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0576434.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHUNN_002060"
     CDS             8330..10048
                     /locus_tag="ACHUNN_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0576435.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHUNN_002061"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHUNN_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0576436.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHUNN_002062"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHUNN_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0576437.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHUNN_002063"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHUNN_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0576438.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHUNN_002064"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHUNN_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0576439.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHUNN_002065"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHUNN_002065"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR0576440.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHUNN_002066"
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHUNN_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR0576441.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHUNN_002067"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHUNN_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0576442.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..>20400)
                     /gene="mdtN"
                     /locus_tag="ACHUNN_002068"
     CDS             complement(19393..>20400)
                     /gene="mdtN"
                     /locus_tag="ACHUNN_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0576443.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4555 a         5435 c         5601 g         4809 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggccagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agccatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggt
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctgcgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
//
LOCUS       DAZOXR010000066        20262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZOXR010000066 DAZOXR010000000
VERSION     DAZOXR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.068"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            60..614
                     /locus_tag="ACHUNN_002069"
     CDS             60..614
                     /locus_tag="ACHUNN_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0576444.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(624..2051)
                     /locus_tag="ACHUNN_002070"
     CDS             complement(624..2051)
                     /locus_tag="ACHUNN_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0576445.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2048..2755)
                     /locus_tag="ACHUNN_002071"
     CDS             complement(2048..2755)
                     /locus_tag="ACHUNN_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0576446.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            2919..3895
                     /gene="ybeQ"
                     /locus_tag="ACHUNN_002072"
                     /pseudo
     CDS             2919..3895
                     /gene="ybeQ"
                     /locus_tag="ACHUNN_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(3965..4447)
                     /locus_tag="ACHUNN_002073"
     CDS             complement(3965..4447)
                     /locus_tag="ACHUNN_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0576447.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4682..7264
                     /gene="leuS"
                     /locus_tag="ACHUNN_002074"
     CDS             4682..7264
                     /gene="leuS"
                     /locus_tag="ACHUNN_002074"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0576448.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7279..7860
                     /gene="lptE"
                     /locus_tag="ACHUNN_002075"
     CDS             7279..7860
                     /gene="lptE"
                     /locus_tag="ACHUNN_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0576449.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7860..8891
                     /gene="holA"
                     /locus_tag="ACHUNN_002076"
     CDS             7860..8891
                     /gene="holA"
                     /locus_tag="ACHUNN_002076"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0576450.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            8893..9534
                     /gene="nadD"
                     /locus_tag="ACHUNN_002077"
     CDS             8893..9534
                     /gene="nadD"
                     /locus_tag="ACHUNN_002077"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0576451.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9558..10169
                     /locus_tag="ACHUNN_002078"
                     /pseudo
     CDS             9558..10169
                     /locus_tag="ACHUNN_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            10429..10746
                     /gene="rsfS"
                     /locus_tag="ACHUNN_002079"
     CDS             10429..10746
                     /gene="rsfS"
                     /locus_tag="ACHUNN_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0576452.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10750..11217
                     /gene="rlmH"
                     /locus_tag="ACHUNN_002080"
     CDS             10750..11217
                     /gene="rlmH"
                     /locus_tag="ACHUNN_002080"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0576453.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11248..13149
                     /gene="mrdA"
                     /locus_tag="ACHUNN_002081"
     CDS             11248..13149
                     /gene="mrdA"
                     /locus_tag="ACHUNN_002081"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0576454.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13152..14264
                     /gene="mrdB"
                     /locus_tag="ACHUNN_002082"
                     /gene_synonym="rodA"
     CDS             13152..14264
                     /gene="mrdB"
                     /locus_tag="ACHUNN_002082"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0576455.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14275..15363
                     /gene="rlpA"
                     /locus_tag="ACHUNN_002083"
     CDS             14275..15363
                     /gene="rlpA"
                     /locus_tag="ACHUNN_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0576456.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15502..16713
                     /gene="dacA"
                     /locus_tag="ACHUNN_002084"
     CDS             15502..16713
                     /gene="dacA"
                     /locus_tag="ACHUNN_002084"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0576457.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16823..17086
                     /gene="ybeD"
                     /locus_tag="ACHUNN_002085"
     CDS             16823..17086
                     /gene="ybeD"
                     /locus_tag="ACHUNN_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0576458.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17187..17828
                     /gene="lipB"
                     /locus_tag="ACHUNN_002086"
     CDS             17187..17828
                     /gene="lipB"
                     /locus_tag="ACHUNN_002086"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0576459.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18088..19041
                     /locus_tag="ACHUNN_002087"
     CDS             18088..19041
                     /locus_tag="ACHUNN_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0576460.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19250..20215
                     /gene="lipA"
                     /locus_tag="ACHUNN_002088"
     CDS             19250..20215
                     /gene="lipA"
                     /locus_tag="ACHUNN_002088"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0576461.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         5185 a         5071 c         5085 g         4921 t
ORIGIN      
        1 ttattgatga gtataaactt gacgctttca aaataaaaag aaaatcgaag cattcacaca
       61 tgaataaaaa atcaatgtat atattcgcaa tttttatagt tgcagcaatt acctgtatta
      121 gccaacccaa gaaaacgacg ttgcgtgata aagccatggt gaattatgcc tttgattatt
      181 taagatcacc aggcagtcta ccgttaacca cggcagccac ggagctttcc gcgattcatg
      241 gtcactcaac atcgcaatat cgccttggcg aattttatct tcatggtagc gacggtaaac
      301 cactggatta tacacaggcg agatactggt atgagcaatc agcagaacag gaaaatccgc
      361 gcgcacaaag caaactgggg tggatctacc tgaaaggcct gggagtcaaa cccgataccc
      421 gtaaagcaat tctctggtat aaggaagcag ctgaacaagg gtatgctcat gctcaatata
      481 ctttgggttt gatctacaga aatggcactg gtattaatgt taaccattat gaatctcaaa
      541 aatggttaaa actggccgcc aaacaacatt acaaaaatgc ggaaagatta cttgccggac
      601 ttcccgcaca ttaacgtcga ctgtcacccg aagagatgga gtatttttcc tatcaaaccg
      661 gcaaagataa aaatgtagaa aataattttg atgacgccca gacagcccag cccgccatcg
      721 tcaacgggca ttggctgcgg ttgcggctcc ggctcgacgg tttcctgacc ggtaaaattc
      781 gccgcctgaa ttaataatcg acgaataatt aatgcttcca gtggacaacg atcttctgca
      841 tcaaggcggc tcacaatcgg cattaatgat tcttctgtcg gttggctggc ctgcactaat
      901 tgtttggcaa ctaaatataa tggccataag ggatgttctg tatcacgcgt gagtgagatc
      961 cgcggcttac gtcctttgtg caaaaccatc tcgaccagtg aacgccccag cgcagagagt
     1021 ttttcatcgc tgagtatttc gtataaatga aaacgcgtac gggcgctgtc gccggggaca
     1081 taaagtagct gtttaccctg atcgttaacc agttcacgag cttcaatcat catcaccact
     1141 aacgggtaat aatcgacccg atgctgacgg caccattgca atattaaagc atccgcctgg
     1201 gtggagggat actcgcgcca gtagtcacaa agttctgcca gcaagctttc gccttctcca
     1261 caatagacgc gttgcgcgta ttcataataa cgcgcatctt cattatcagg ttcagcttcc
     1321 cgccacgacc gggcgtaatc cagaagttcg gggatatcca ggcgacctgc gctataccag
     1381 cggagtaaat ttctgtgcag ttttgcatca tcggggatga tcactgcgcg atgttgcgct
     1441 aaatacaatg tcagccattc tggatgcgat gaccaaatcc gagcgaacat ttggtaaaaa
     1501 tcgatggtgt cattttgcac ggcaacgggc agatgcagaa tggtctggaa gttaaaaaca
     1561 ttccccttcg caatggcata taaaaaggat tccagttctt cttcgtctat ttccccgacg
     1621 ttttcctcct gcaaccaccg caatctttct gctaataaca ccacgcactc gaaggataaa
     1681 tgggcagtgt tcatggcgat tgtgcacagc gaccagcgta attcatcaat ctcttccatc
     1741 gagcaataat ttaattgctg aatgaatcgc tgccaggcgg agggcagaaa acgttcgcta
     1801 tcagaggcaa gtaacgcgcg aaaggcgagc agaatttcat gttctgctac ctcatattct
     1861 tctggttgcc aaacagattt agcaggcgac tgcgcaatcc ggagcgcctc ctcatacgcc
     1921 tgacgaagtt gtttaaaacc ctgcggatcg gtttctggat gaaaggacag taacagcgcc
     1981 agataagcgc ggcggataat atcgacgtca gtcgtttcct ctatttcgag gatcttccag
     2041 caatttttca taggtcgctc atatccttaa gcgtttccgg gcatgtgggg tagtcagcca
     2101 gtggttgcca ggacaaggca gggtaatacg catcatcact ggcaaataat tgataaaaca
     2161 tatcattgcc ggaatcgtcg tattctttac gagctaagaa ttcgcgtaat aaaggcagat
     2221 gatcatcttc gggagactgc caatattgcc gaccaaaggt ataaccggca aagtattgcc
     2281 gccagttact ataataacgt agagcccgta gatgaatacg cgactggatc catagacttt
     2341 cttcttcact caaccagtta ttaagtacgc ccattcgact taaaaaaccc atccggacat
     2401 aatcccatgc tttaattcct ccctcaccac agcattcggc tgtgctggcg acaaattgcg
     2461 cataagcctg accttgctca tttagttcag caagatagtc acgccattca catggcgaat
     2521 aacgaaacca gcggtgataa aaccctgcca accgagcggc atgaccgtta tcgatcattc
     2581 ggtgaattgt gcgcagcagg tcatcacggt catcaattcc ccatgaggaa gaaaggtcaa
     2641 aaaagcatgt acctaaatac attttggggg atgaatgcga aggtaaacag cattcattgc
     2701 gtatttccat cggagtggaa agtgcaaaca atattttttg cgaccccatg tccatataat
     2761 ttatccttaa acattcattt aatgaatgta aactacattt cgagactcag ttatttatac
     2821 ttttctgacg caacataata cgcataaaat aaggcttacg caaaaatgga acacataaaa
     2881 atataatatg ttttatctac aaccataagg aaatgataat ggtttttacg tcaagttgct
     2941 gcgataattt atcaatagat gagattatcg aacgtgctga aaaaggcgat tgcgaggctc
     3001 aatatattgt tgggttttat tataatcgcg atagcgcaat tgactctccg gacgacgaaa
     3061 aggcctttta ctggctgaag ctggccgctg agcaaggtca ttgtgaagca cagtattcct
     3121 tagggcaaaa gtataccgag gataaaagcc gtcataaaga taatgagcaa gccatcttct
     3181 ggctgaaaaa agctgcccta caaggccata ctttcgcttc caacgccctt ggctggacac
     3241 tggatcgtgg agaagccccc aattataaag aagcggttgt ctggtatcag atagccgcgg
     3301 agagcggaat gtcttatgcg caaaataatc ttgggtggat gtacagaaat ggcaacggag
     3361 tcgcaaaaga ctatgcgctg gcatttttct ggtacaaaca agctgcatta caaggccata
     3421 gtgacgcgca aaacaatctg gccgatcttt atgaagacgg aaaaggcgtt gctcaaaaca
     3481 agacactcgc cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg cacgcccagt
     3541 ttcaaattgc atgggattat aacgctggcg aaggggtgga ccaggactat aagcaagcga
     3601 tgtactggta tctgaaggct gccgctcagg aaagcgtcgg cgcttacgtc aacatcggtt
     3661 atatgtataa acacggacaa ggcgttgaga aagattatca ggctgccttt gaatggttta
     3721 cgaaagccgc tgaatgcaat gacgccactg cctggtataa cctggccatt attatcacta
     3781 cggagaagga agacctgtcg atctccgaca ggctctcgac ctgtatcgta aagttcagtc
     3841 atccggaacc agagatgtca gtcaggaaat tcgtgagact gaagatttac tgtagagata
     3901 aaaacaaggg ccggggaatt tcattcggcc catattctct ttattgctgt gcgaaatcag
     3961 actattacgg ctcaaacggg cgtctctgga actggtcatg accacatttc gggcatagcg
     4021 tcagcacttc cggtgtgtag atcgggagat ggaagtgaca tttctcgcag accagatttc
     4081 ccagcccgac cacttctccg ctgtgataaa ccccatgatg attgaggtcc tggaaaactt
     4141 cgcgccattc aagctgcgtt ttatcggtga tatctgccag ctcctgccat aagctttctt
     4201 taatcacccg cataaagacg ctgtcagatt cttctttcag gctctcttca tagctcatgg
     4261 cgaactcttc caggtcacgt cttacagctc gcgtcagctc atcgacctcg gttcgcgtta
     4321 actcccctgt ttttattacg cgctcgcgcg cctgttccac cagtgcgtcg atatcacgtt
     4381 cgccattgcg caggcgttcg ctcagtgacg cgaccagttc acggtaatat tgagcaacct
     4441 tgttcatcgt ttcatctccc gggtcgttaa ctgactataa taatagacgt tctttgttcc
     4501 ttgctttgct aatacgccca caaactctgt aaattaatgt atgaataatc cgggcatcat
     4561 ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg tttgggctat gctatgcgga
     4621 tctgaaaaac cacctcaacg ctacatttgt agccgtattg aaaacaggac cactggctgc
     4681 catgcaagag caataccgcc cggaagagat agaatccaaa gtacagcttc attgggatga
     4741 gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag aagtattact gcctgtctat
     4801 gcttccctat ccttctggtc gactacacat gggccacgta cgtaactaca ccatcggtga
     4861 cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc ctgcagccga tcggctggga
     4921 cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac aacaccgctc cggcaccgtg
     4981 gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa atgctgggct ttggttatga
     5041 ctggagccgc gagctggcaa cctgtacgcc ggaatactac cgttgggaac agaaattctt
     5101 caccgagctg tataaaaaag gcctggtata taagaagact tctgcggtca actggtgccc
     5161 gaacgaccag accgtactgg cgaacgaaca agttatcgac ggctgctgct ggcgctgcga
     5221 taccaaagtt gaacgtaaag agatcccgca gtggtttatc aaaatcactg cttacgctga
     5281 cgagctgctc aacgatctgg ataaactgga tcactggcca gacaccgtta aaaccatgca
     5341 gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc ttcaacgtta acgactatga
     5401 caacatgctg accgtttaca ctacccgccc ggacaccttt atgggttgta cctacctggc
     5461 ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa aataatcctg aactggcggc
     5521 ctttattgac gaatgccgta acaccaaagt tgccgaagct gaaatggcga cgatggagaa
     5581 aaaaggcgtc gatactggct ttaaagctgt tcacccatta acgggcgaag aaattcccgt
     5641 ttgggcagca aacttcgtat tgatggagta cggcacgggc gcagttatgg cggttccggg
     5701 tcacgaccag cgcgactacg agtttgcctc taaatacggc ctgaacatca aaccagttat
     5761 cctggctgct gacggctctg agccagatct ttctcagcaa gccctgactg aaaaaggcgt
     5821 gctgttcaac tctggcgaat tcaacggtct tgaccatgaa gcggccttca acgccatcgc
     5881 cgataaactg actgcgatgg gcgttggcga gcgtaaagtg aactaccgcc tgcgcgactg
     5941 gggtgtttct cgtcagcgtt actggggcgc gccgattccg atggtgacgc tggaagacgg
     6001 taccgtaatg ccgaccccgg acgaccagct gccggtgatc ctgccggaag atgtggtgat
     6061 ggacggcatt accagcccga ttaaagcaga tccggagtgg gcaaaaacta ccgttaacgg
     6121 tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt atggagtcct cctggtacta
     6181 tgcgcgctac acttgcccag agtacaaaga aggtatgctg gattccgaag cggctaacta
     6241 ctggctgccg gtggatatct acattggtgg tatcgaacac gccattatgc acctgctcta
     6301 cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg gtgaactctg acgaaccagc
     6361 gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc ttctactatg ttggcgaaaa
     6421 cggcgaacgt aactgggttt ccccggttga tgctatcgtt gaacgtgacg agaaaggccg
     6481 tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt tataccggca tgagcaaaat
     6541 gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg gttgaacgtt acggcgcgga
     6601 caccgttcgt ctgtttatga tgtttgcttc tccggctgat atgactctcg aatggcagga
     6661 atccggtgtg gaaggggcta accgcttcct gaaacgtgtc tggaaactgg tttacgagca
     6721 cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg ctgactgaag atcagaaagc
     6781 gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc gatgatatcg gccgtcgtca
     6841 gaccttcaac accgcaattg cggcgattat ggagctgatg aacaaactgg cgaaagcacc
     6901 aaccgatggc gagcaggatc gcgctctgat gcaggaagcg ctgctggccg ttgtccgtat
     6961 gcttaacccg ttcaccccgc acatctgctt cacgctgtgg caggaactga aaggcgaagg
     7021 cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa gcgatggtgg aagactccac
     7081 gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa atcaccgttc tggtggacgc
     7141 aacggaagaa caggttcgcg aacgtgctgg ccaggaacat ctggtagcaa aatatcttga
     7201 tggcgttacc gtgcgtaaag tgatttacgt accaggtaaa ctcctcaatc tggtcgttgg
     7261 ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt tgttatctct ggcggtgtta
     7321 atcaccgccg ggtgtggctg gcatctgcgt gataccacgc aggttccttc cactatgaag
     7381 gtcatgatcc tggactcagg cgatccgaac gggccattaa gccgtgcagt gcgtaaccag
     7441 ttacgtctga atggtgtcga gttgcttgat aaagaaacca cgcgtaagga cgttccatcc
     7501 ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat cggtattccg taacggtcaa
     7561 acagcagagt atcagatgat catgacggtg aatgcgaccg tgttgatccc cggccgtgat
     7621 atctacccga ttagcgccaa agtcttccgt tcgttctttg ataacccgca aatggcgtta
     7681 gcgaaagata acgaacaaga catgatcgta aaagagatgt acgaccgtgc tgccgaacag
     7741 ctgattcgta agctgccaag catccgtgct gcggatattc gttccgacga agaacagacg
     7801 tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct ccaccacgct gggtaactga
     7861 tgattcggtt gtacccggaa caactccgcg cgcagctcaa tgaagggctg cgcgcggcgt
     7921 atcttttact tggtaacgat cctctgttat tgcaggaaag ccaggacgct gttcgtcagg
     7981 tagctgcggc acaaggattc gaagaacacc acactttttc cattgatccc aacactgact
     8041 ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt tgccagtcga caaacgctat
     8101 tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa tgaacaactt ctcacactca
     8161 ccggacttct gcatgacgac ctgctgttga tcgtccgcgg taataaatta agcaaagcgc
     8221 aagaaaatgc cgcctggttt actgctcttg cgaatcgcag cgtgcaggtg acctgtcaga
     8281 caccggagca ggctcagctt ccccgctggg ttactgcgcg cgcaaaacag ctcaacttag
     8341 aactggatga cgcggcaaat caggtgctct gctactgtta tgaaggtaac ctgctggcgc
     8401 tggctcaggc actggagcgt ttatcgctgc tctggccaga cggcaaattg acattaccgc
     8461 gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc ttttcattgg gttgatgctt
     8521 tgttgatggg aaaaagtaag cgcgcattgc atattcttca gcaactgcgt ctggaaggca
     8581 gcgaaccggt tattttgttg cgcacattac aacgtgaact gttgttactg gttaacctga
     8641 aacgccagtc tgcccatacg ccactgcgtg cgttgtttga taagcatcgg gtatggcaga
     8701 accgccgggg catgatgggc gaggcgttaa atcgcttaag tcagacgcag ttacgtcagg
     8761 ccgtgcaact cctgacacga acggaactca ccctcaaaca agattacggt cagtcagtgt
     8821 gggcagagct gaaagggtta tctcttctgt tgtgccataa acccctggcg gacgtattta
     8881 tcgacggttg atatgaaatc tttacaggct ctgtttggcg gcacctttga tccggtgcac
     8941 tatggtcatc taaaacccgt ggaaacgctg gcgaatttga ttggtctgac gcgggtcaca
     9001 atcatcccta ataatgttcc tccgcatcgt ccccagccgg aagcgaacag cgtgcagcgt
     9061 aaacacatgc ttgaactggc gattgccgac aagccattat ttactcttga tgaacgcgag
     9121 ctaaagcgca atgccccctc ttacactgcg caaacactga aagagtggcg gcaggaacaa
     9181 ggaccggacg tgccgctggc gtttattatt ggtcaggatt cactgctgac ctttccgacc
     9241 tggtacgaat acgaaacgat actcgacaat gcacatttga tcgtctgtcg gcgtccaggt
     9301 tacccacttg aaatggcgca accgcaatac cagcaatggc tggaagatca tttgacacat
     9361 aacccggaag atcttcacct tcagcctgcc ggtaaaattt atctggctga aacgccgtgg
     9421 tttaacatct cggcgaccat catccgcgaa cgtttgcaaa acggtgaatc gtgtgaggat
     9481 ttattgccgg aaccggtact gacttacatt aaccaacaag gcttgtatcg ctgatacctg
     9541 tcgttacgga gggcatgatg cgactgtggt taattcgtca tggtgaaacg caagcgaata
     9601 tcgatggtct ttacagcggt catgcgccca cccccctgac cgcgcgcggt attgagcaag
     9661 cgcaaaatct gcatacgctg ctacatggtg tttcctttga tctggtttta tgcagtgaac
     9721 tggaacgggc acagcatacc gcacgactgg ttctcagtga ccgccagctc cccgtgcaaa
     9781 tcatccctga actcaacgaa atgttttttg gcgactggga gatgcgacat catcgcgacc
     9841 tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa tgactggcag catgcaatcc
     9901 ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt ggaacgcttt atcgcaaggc
     9961 ttagtgaatt tcagcactat cagaatattt tagtcgtcag ccatcagggt gtactgagtc
    10021 tgttaatcgc ccgtttaatt ggcatgcctg ccgaggctat gtggcatttt cgcgttgact
    10081 aggggtgttg gagcgccatt gatatcaacc aaaaattcgc gacgctacgc gtcctcaata
    10141 gccgtgccat cggggtcgag aatgcatgac ttttctgttt ttttacgggt aagccgcaac
    10201 gaccattgac agacccgggc aggctgatat tctccgcagc cagacttttt tcgccagaca
    10261 cgactttgta gaaattgttt tacaaaaatg gcgatgcaat ctgcggcgcg gggtgggatg
    10321 atagcccact ttcgaaagcc gattcggcga caattgtccc gaaatcgcct ctggttcagg
    10381 tatactgaca gaccattttt atctatttga ttcacccagg gggaaaactt gcagggtaaa
    10441 gcactccagg attttgttat cgacaaaatt gatgacctca aaggtcagga catcatcgcc
    10501 ttagacgttc aaggcaaatc cagcatcacc gactgcatga tcatctgtac gggtacgtcc
    10561 agccgtcatg ttatgtccat tgctgaccac gttgtgcagg agtctcgcgc agcgggcctg
    10621 ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga ttgtcgtgga tttgggcgat
    10681 gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt atgaactgga aaaactctgg
    10741 agttaatgcg tgaagctgca acttgtcgcc gtgggaacga aaatgccgga ctgggtacaa
    10801 accggtttta ccgagtacct gcgtcgtttt ccgaaagata tgcccttcga gctgattgaa
    10861 attccggccg gaaaacgcgg caagaatgcg gacatcaagc gcatactcga caaagagggt
    10921 gagcagatgt tggcggccgc aggcaaaaac cgcattgtca ccctcgatat tccaggcaag
    10981 ccctgggata cgccgcagtt agccgctgag ctggaacgct ggaagctgga tggtcgcgac
    11041 gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg cctgtaaagc ggcggcggag
    11101 cagagctggt cgctgtcggc gcttaccctc ccccatccgc tggttcgcgt gctggtcgca
    11161 gagagtctgt accgggcgtg gagcatcacc accaaccatc cttatcaccg tgagtgataa
    11221 gggagctttg agtagaaaac gcagcggatg aaactacaga actcttttcg cgactatacg
    11281 gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct ttttggggat tttgctgctg
    11341 accggcgtgc ttatcgccaa cctgtataat ctgcaaattg ttcgctttac cgactaccag
    11401 actcgctcta atgaaaaccg cattaagctg gtgcctatcg cgcccagccg cggcattatc
    11461 tacgaccgta atggtatccc tctggccctc aaccgcacta tctaccagat agaaatgatg
    11521 ccagagaaag tcgataacgt gcagcaaacg ctggacgctt tgcgcagcgt ggttgatctg
    11581 accgatgacg atattgctgc attccgaaaa gagcgcgcac gttcacaccg tttcacctct
    11641 attccggtga aaactaacct gaccgaagta caagtagctc gctttgccgt caatcagtac
    11701 cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt actatcctta cggttcggcg
    11761 ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata aagacgtcga acgcctgaat
    11821 aatgacggca aactggccaa ctatgcggca acgcatgata tcggtaagct gggcattgag
    11881 cgttactatg aagacgtgct gcacggtcag accggttatg aagaggttga agttaacaac
    11941 cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc aagccggaca cgatatttac
    12001 ctgacgctgg atctcaaact ccagcaatat attgaaacgc tgctggcggg tagccgcgca
    12061 gctgtggttg tcaccgatcc gcgtacaggt ggggtgctgg cgctggtttc cacgcctagt
    12121 tatgacccaa acctgtttgt tgacggtatc tccagcaaag attattccgc cttgttgaac
    12181 gccccgaata caccgctggt gaaccgcgcc acacaggggg tttatcctcc cgcgtctaca
    12241 gttaaaccct atgtggcggt ttcggcattg agcgccgggg tgatcacgcg caatacgacg
    12301 ctgtttgacc caggctggtg gcaactgcca ggttcggaaa aacgttatcg tgactggaaa
    12361 aaatggggcc acgggcgtct gaatgtcaca agatcgctgg aagaatctgc ggataccttc
    12421 ttctatcagg tggcctacga tatggggatc gatcgcctct ccgaatggat gggtaaattc
    12481 ggttatggtc attacaccgg tatcgacctg gcggaagaac gttccggcaa catgcctacc
    12541 cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc agggtgacac cattccggtt
    12601 ggtatcggtc agggttactg gacagcgacc ccaatccaga tgagtaaggc actgatgatc
    12661 ctgattaatg acggtatcgt gaaggttcct catttgctga tgagcaccgc cgaagacggc
    12721 aaacaggtgc catgggtaca gccgcatgaa ccgcccgtcg gcgatattca ttccggttac
    12781 tgggagctgg cgaaagacgg tatgtacggt gttgctaacc gccctaacgg tacggcgcat
    12841 aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat ccggtaccgc tcaggtcttc
    12901 ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg ccgagcgttt acgtgaccac
    12961 aaactgatga ccgcctttgc gccatacaac aatccgcaag tggctgtcgc catgattctg
    13021 gagaacggtg gtgcgggtcc ggcggttggt acactgatgc gccagatcct cgaccacatt
    13081 atgctgggtg ataacaacac cgatctgcct gcggaaaatc cagcggttgc cgcagcggag
    13141 gaccattaat catgacggat aatccgaata aaaaaacatt ctgggataaa gtccatctcg
    13201 atcccacaat gctgctgatc ttactggcat tgctggttta cagcgccctg gttatctgga
    13261 gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat cggccaaatc gcgatgggtc
    13321 tagtcatcat ggtggtgatg gcgcaaattc ctccacgcgt ttatgaaggc tgggccccct
    13381 atctctatat catctgtatt attttgctgg tggcggtaga tgctttcggt gccatctcta
    13441 aaggtgctca acgctggctg gacctcggta ttgttcgttt tcagccgtcg gaaattgcca
    13501 aaatagccgt accactgatg gttgcgcgct ttatcaaccg cgacgtttgc ccgccatcgt
    13561 tgaagaacac tggcatcgcg ctggtgctga tatttatgcc cacgctgctg gtggctgcac
    13621 agcctgacct gggaacatca atcctcgttg cgctttccgg tctgtttgta ctgttcctct
    13681 ctggccttag ctggcgtctg attggcgtcg cagtagtgct ggtggcggcg ttcattccga
    13741 ttctgtggtt cttcctgatg catgattacc agcgccagcg cgtaatgatg ctcctagacc
    13801 cggaatcaga cccactcggc gcgggctatc acattattca gtctaaaatt gctattggct
    13861 ccggcggatt acgcggcaaa ggctggctgc acggcactca gtcacagctt gaatttctcc
    13921 ccgaacgcca taccgacttt atcttcgcgg tactggcgga agagctggga ttagtgggca
    13981 ttctgattct gctcgctctc tacattctgc tgatcatgcg cgggctgtgg atagccgcca
    14041 gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt aatgctgata ttattcgttt
    14101 atgtcttcgt aaatattggt atggtaagcg gtattctgcc ggttgtaggg gttccgctcc
    14161 cactggtcag ttatggagga tcggcgctaa ttgtgctgat ggctgggttc gggattgtaa
    14221 tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt gtaagaggtg cgcaatgcgt
    14281 aagcagtggc tcgggatctg catcgcggca ggaatgctcg cggcatgtac aagcgatgat
    14341 ggtcagcaac agacggtaag tgtaccgcag cctgcggtat gtaacggccc catagttgaa
    14401 attagcgggg cggacccgcg tttcgaacca ctgaacgcga cggcaaatca ggattaccag
    14461 cgcgacggta aaagctacaa aatcgtgcag gatccgtctc gatttagcca ggcgggactg
    14521 gcggcaatct atgatgccga acccggcagt aacctgacgg cctctggcga agctttcgat
    14581 ccgacacagc tgacggcggc ccatccaacg cttccgatcc ccagctacgc cagaatcact
    14641 aacctggcta acgggcgaat gatcgtggtg cgcattaatg atcgcggccc ttacggcaac
    14701 gaccgcgtta tttcactttc tcgcgcggca gctgaccgtc ttaacacgtc aaacaacacc
    14761 aaagttcgta tcgatccgat tattgtcgcc caggatggtt cgctttctgg tcctggtatg
    14821 gcttgtacca cagtcgccaa acagacttac gccctgccag cacctcccga tttaagcggt
    14881 ggcgcgggaa caagttcagt gtctggcccg cagggtgaca ttcttccggt cagtaattcg
    14941 acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca gtagcggttt cctcggcgct
    15001 ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac cgacgcctgc gccacagccc
    15061 gttgttacag ctccgtcgac aacgcctgca acctcgcctg caatggtgac accgcaagcc
    15121 gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg gggccgtaag cgatcaggct
    15181 cgtgcgcaac agtaccaaca gcaactggga cagaagttcg gcgtccccgg tcgcgtaact
    15241 caaaatggcg cggtctggcg gatccagctt ggcccattcg ccagcaaagc cgaagccagt
    15301 accttgcagc aacgtttgca aaccgaagcc caattacagt catttattac taccgcgcag
    15361 tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat gacgttaaca aagtcacgcg
    15421 gaaagtcaga tgcctgccgg tagtgcattt gctatagtag ggcacttttt taattccatc
    15481 acggatgtcg tagttcagac catgaatacc attttttccg ctcgtatcat gaagcgcctg
    15541 gcgctcacca cggctctttg cacagccttt atctctgctg cacatgccga tgacctgaat
    15601 atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg agtcctacat cctgattgac
    15661 tataactccg gcaaagtgct cgccgaacag aacgcagatg tccgccgcga tcctgccagc
    15721 ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa tgaaagccgg taaatttaaa
    15781 gaaactgatt tagtcactat cggcaacgac gcatgggcca ccggtaaccc ggtgtttaaa
    15841 ggttcttcgc tgatgttcct caaaccgggc atgcaggttc cggtttctca gctgatccgc
    15901 ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca tggccgattt tgccgctggt
    15961 agccaggacg cttttgttgg cttgatgaac agctacgtta acgcactggg cctgaaaaat
    16021 acccacttcc agacggtaca tggtctggat gctgatggtc agtacagctc cgcgcgagat
    16081 atggcgctga tcggccaggc attgatccgt gacgtaccga atgaatactc gatctataaa
    16141 gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc gtaacggcct gttatgggat
    16201 aacagcctga atgtcgacgg catcaaaacc ggacacactg acaaagcagg ttacaacctt
    16261 gttgcttctg cgactgaagg ccagatgcgc ttgatttctg cggtgatggg cggacgtact
    16321 tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct ggggcttccg tttcttcgaa
    16381 accgttaacc cactgaaagt aggtaaagag ttcgcctctg aaccggtttg gtttggtgat
    16441 tctgatcgcg cttcgttagg ggttgataaa gacgtgtacc tgaccattcc acgtggtcgc
    16501 atgaaagatc tgaaagccag ctatgtgctg aacagcagtg aattgcatgc gccgctgcaa
    16561 aagaatcagg tcgtcggaac tatcaacttc cagcttgatg gcaaaacgat cgagcaacgc
    16621 ccgctggttg tgttgcaaga aatcccggaa ggtaacttct tcggcaaaat cattgattac
    16681 attaaattaa tgttccatca ctggtttggt taaaaattga acacttgaaa gtgtaatttc
    16741 cgtccccata tactaagcat cagtaaaaaa ctcccgcctt ctggcgggag ttgctattta
    16801 attacgttac gccggagctg acatgaaaac caaacttaac gaactgcttg aattccctac
    16861 tccttttact tacaaagtta tggggcaggc gttacctgag ctggttgatc aggtggttga
    16921 agtggtacag cgccatgcgc caggtgacta caccccaacg gtaaaaccaa gcagcaaagg
    16981 caactaccac tcggtatcta tcactatcaa cgccactcat atcgagcagg ttgaaacact
    17041 gtatgaagaa ctaggcaaaa tcgatattgt ccgcatggtt ctgtaactcg cttctccgtt
    17101 acccggcccc tggtcgggta actcccctct cctcgctgtg atatactttt cccccacttt
    17161 tactcattct ccacggagat gcagttttgt atcaggataa aattcttgtc cgccagctcg
    17221 gtcttcagcc ttacgagcca atctcccagg ctatgcatga attcaccgat acccgcgatg
    17281 atagtaccct tgatgaaatc tggctggtcg agcactatcc ggtattcacc caaggtcagg
    17341 caggaaaagc ggagcacatt ttaatgccgg gtgatattcc ggtgatccag agcgatcgcg
    17401 gtgggcaggt gacttatcac gggccggggc aacaggtgat gtatgtgttg cttaacctga
    17461 aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct tgagcaaaca gtggtgaata
    17521 ccctggctga actgggtata gaagcgcatc ctcgggctga cgcgcctggt gtctatgtcg
    17581 gggaaaagaa aatttgctca ctgggtttac gtattcgacg cggttgttca ttccacggtc
    17641 tggcattaaa cgtcaatatg gatctttcac catttttacg tattaatcct tgtgggtatg
    17701 ccggaatgga aatggctaaa atatcacaat ggaaacccga agcgacgact aataatattg
    17761 caccacgttt actggaaaat attttagcgc tactaaacaa tcctgacttc gaatatatta
    17821 ccgcttaatt gcatacctca atggcccaat ttacactggg tcattactca taatacctgc
    17881 taatgcttta catctccttc gcgtttgtat actttcttta tcacttgcag aaatgcggtg
    17941 ctacgccttg ctccgtaact caccccattg attaacctat tgaaatagtt gattatggtg
    18001 agcaaaagcg ttccgttcca attagacata agattattgt aattttttta aatattattt
    18061 ttccatgaat aaaattggag taagtgcgtg gatagtaata atcaaattga accctgttta
    18121 agcagaaagt cgtcagaagg taaaccacaa atatttacaa ccttacgaaa tattgatctt
    18181 aaccttctga ctatttttga agctgtatat gtacataaag ggatcgttaa tgcagcgaaa
    18241 gtgcttaatc tgaccccctc ggcaatcagt cagtctattc agaaactgcg cgttatattc
    18301 cctgacccat tgtttattcg caaaggccag ggtgtcactc ctaccgcatt tgcgatgcat
    18361 ctacatgagt atatcagtca gggccttgag tccattcttg gcgcgctgga tatcgaagga
    18421 agctatgata agcaacgaac gataactatt gcgaccactc cctcggtcgg agccctggtc
    18481 cttcctgtca tctatcgggc gattaaaact cactatccgc agcttttact gcgcaacccg
    18541 cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa ccgatctcat catcgataac
    18601 atgttttgca ccaatcgtac ggtgcaacat catgttctgt tcaccgacaa tatggtgtta
    18661 atttgccgcg agggaaatcc actactctct ttagaagatg acagagagac tatcgacaac
    18721 gctgcgcatg tactcctgtt accggaaggg caaaatttca gcggtctgcg gcagagagtt
    18781 caagagatgt ttccggaccg gcaaatcaat ttcaccagct acaacatttt gacaatcgct
    18841 gcactggttg ccaacagtga catgttagcg attattccca gccgttttta taacctgttt
    18901 agtcgttgct ggccgctgga aaaactgcct tttccgtcct taaatgagga gcaaatagat
    18961 ttctctatcc actacaacaa attcagcctg cgtgatccca tcctgcacgg ggtaatagat
    19021 gttatccgta atgcattttg aaaaagtatt gttacagcca gatcacagcg cagaacgata
    19081 aaatggcaca atccaacaac aattttacat tttcgcgacc gctttggctg ctttcaggtc
    19141 cgtttcaatg atatactgcc agtcgttaat tcaaaaatag ttgataatta caacaatcta
    19201 ttgaattgaa acgctttcct tcgtaattcg caactggaac acgcacgcta tgagtaaacc
    19261 cattgtgatg gaacgcggtg ttaaataccg cgatgccgat aagatggccc ttatcccggt
    19321 taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa
    19381 gcttccagcg gactctacac gtatccaggg catcaaagcc gcaatgcgca aaaatggcct
    19441 gcattctgtc tgcgaggaag cctcctgccc taacctggcg gaatgcttca accacggcac
    19501 agcaacgttt atgatcctcg gcgctatttg tacccgccgt tgtccgttct gtgatgttgc
    19561 ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg aaactggcgc agaccattgc
    19621 cgatatggcg ctgcgttatg tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg
    19681 cggtgcccag cactttgcgg attgcattac tgccattcgg gaaaaaagcc cgcaaatcaa
    19741 aattgaaact ctggtgccgg atttccgtgg tcgtatggat cgtgctctgg atattctgac
    19801 tgcaacgcca ccagatgtgt tcaaccataa cctggaaaac gtaccgcgta tttaccgtca
    19861 ggtacggcct ggtgcagatt acaactggtc gctgaagctg ctggaacgct ttaaagaagc
    19921 gcatccggaa atcccgacca agtctggtct gatggtggga ctgggtgaaa ccaatgaaga
    19981 aattattgag gtaatgcgcg acctgcgccg tcatggtgtg acgatgttaa cgctggggca
    20041 atatttgcag ccaagccgcc atcacctgcc ggttcaacgt tacgttagcc cggatgagtt
    20101 cgacgaaatg aaagccgaag cgctggcgat gggctttacc catgctgcat gcggtccgtt
    20161 tgtccgctct tcttaccacg ccgatttgca ggcgaaaggg atggaagtta agtaatcctg
    20221 taataattac ttacatctgt ctgataaaaa acccgctttt tg
//
LOCUS       DAZOXR010000067        20004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZOXR010000067 DAZOXR010000000
VERSION     DAZOXR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.069"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..70
                     /locus_tag="ACHUNN_002089"
     CDS             <1..70
                     /locus_tag="ACHUNN_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFR0576462.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHUNN_002090"
     CDS             complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHUNN_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0576463.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            345..791
                     /locus_tag="ACHUNN_002091"
     CDS             345..791
                     /locus_tag="ACHUNN_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0576464.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHUNN_002092"
     CDS             complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHUNN_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR0576465.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1666..2847
                     /gene="nimT"
                     /locus_tag="ACHUNN_002093"
     CDS             1666..2847
                     /gene="nimT"
                     /locus_tag="ACHUNN_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR0576466.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2902..3249
                     /locus_tag="ACHUNN_002094"
     CDS             2902..3249
                     /locus_tag="ACHUNN_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0576467.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHUNN_002095"
     CDS             complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHUNN_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0576468.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3708..4733
                     /gene="dgcP"
                     /locus_tag="ACHUNN_002096"
     CDS             3708..4733
                     /gene="dgcP"
                     /locus_tag="ACHUNN_002096"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0576469.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4766..4864
                     /locus_tag="ACHUNN_002097"
     CDS             4766..4864
                     /locus_tag="ACHUNN_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0576470.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4867..4941
                     /gene="yoaJ"
                     /locus_tag="ACHUNN_002098"
     CDS             4867..4941
                     /gene="yoaJ"
                     /locus_tag="ACHUNN_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0576471.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(5000..5248)
                     /locus_tag="ACHUNN_002099"
     CDS             complement(5000..5248)
                     /locus_tag="ACHUNN_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0576472.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5396..5578)
                     /locus_tag="ACHUNN_002100"
     CDS             complement(5396..5578)
                     /locus_tag="ACHUNN_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR0576473.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5582..5941)
                     /gene="yeaR"
                     /locus_tag="ACHUNN_002101"
     CDS             complement(5582..5941)
                     /gene="yeaR"
                     /locus_tag="ACHUNN_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR0576474.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6114..6752)
                     /gene="leuE"
                     /locus_tag="ACHUNN_002102"
     CDS             complement(6114..6752)
                     /gene="leuE"
                     /locus_tag="ACHUNN_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR0576475.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6879..7802)
                     /gene="dmlR"
                     /locus_tag="ACHUNN_002103"
     CDS             complement(6879..7802)
                     /gene="dmlR"
                     /locus_tag="ACHUNN_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR0576476.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7905..8990
                     /gene="dmlA"
                     /locus_tag="ACHUNN_002104"
     CDS             7905..8990
                     /gene="dmlA"
                     /locus_tag="ACHUNN_002104"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR0576477.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9241..10851
                     /gene="yeaV"
                     /locus_tag="ACHUNN_002105"
     CDS             9241..10851
                     /gene="yeaV"
                     /locus_tag="ACHUNN_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR0576478.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10883..12007
                     /gene="yeaW"
                     /locus_tag="ACHUNN_002106"
     CDS             10883..12007
                     /gene="yeaW"
                     /locus_tag="ACHUNN_002106"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR0576479.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12063..13028
                     /gene="yeaX"
                     /locus_tag="ACHUNN_002107"
     CDS             12063..13028
                     /gene="yeaX"
                     /locus_tag="ACHUNN_002107"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0576480.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13082..14197)
                     /gene="rnd"
                     /locus_tag="ACHUNN_002108"
     CDS             complement(13082..14197)
                     /gene="rnd"
                     /locus_tag="ACHUNN_002108"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0576481.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14279..15964)
                     /gene="fadD"
                     /locus_tag="ACHUNN_002109"
     CDS             complement(14279..15964)
                     /gene="fadD"
                     /locus_tag="ACHUNN_002109"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0576482.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16169..16750)
                     /gene="yeaY"
                     /locus_tag="ACHUNN_002110"
     CDS             complement(16169..16750)
                     /gene="yeaY"
                     /locus_tag="ACHUNN_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0576483.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16790..17485)
                     /gene="tsaB"
                     /locus_tag="ACHUNN_002111"
     CDS             complement(16790..17485)
                     /gene="tsaB"
                     /locus_tag="ACHUNN_002111"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0576484.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17543..19453)
                     /locus_tag="ACHUNN_002112"
     CDS             complement(17543..19453)
                     /locus_tag="ACHUNN_002112"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0576485.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19585..19929
                     /locus_tag="ACHUNN_002113"
     CDS             19585..19929
                     /locus_tag="ACHUNN_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0576486.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4848 a         5190 c         4909 g         5057 t
ORIGIN      
        1 tattttgaaa accacggatt atttgcgcat tgcgcaacca gaactgctca atttccgccg
       61 cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga caaaaccagc acaacagcaa
      121 tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa catttgatcg ttcatagcat
      181 gcccccggga gatagctgtt ttcacgttat tgttagcgtg cacaaatggc agtttgatga
      241 caattcgcct gattttttat gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa
      301 acgtaaagta agcaggctta ttattttttg gcaaggaaac cacgatgttt gatgtcactt
      361 tgctgatcct gctcgggtta gctgcgctgg gctttatcag tcataacacc actgtcgccg
      421 tttcaattct ggtgttgatc attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga
      481 ttgaaaaaca ggggctaagt atcgggataa tcattctgac gattggcgtt atggccccca
      541 tcgccagcgg gacgctaccg ccctcaacct tgattcactc gtttctgaac tggaaatcgc
      601 tggtggcaat tgcagtaggc gtgattgtct cctggctggg tgggcgcggc gtgaccttaa
      661 tgggcagcca gccgcaactg gtcgccgggc tgctggtagg cactgtttta ggcgtggcgc
      721 tgtttcgcgg cgtaccggtc ggcccgctta ttgccgcggg tctggtttcg ctgattgtgg
      781 ggaaacagta gttaatcccg caatatatcg gcctggcgtt tgccccaggc ctttcttaaa
      841 catagtaata aaggccgttg ttgaatcata ccccagcgta tgagcaactt tctgtacagt
      901 atcgcctttc actaatccct gaagtgccat aatcagctgt aactgctgac gccactggcg
      961 aaaactcaac cccgtctctt ttactatcag tcgcgcaaga ttgcgttcac tcatcgcaaa
     1021 aaagccagcc cattgcccta atgaccccca ctcgacgggc cctttcgcca tcatctccac
     1081 catcgtgcgg attttaggat gagaagaaac gggcaggtgc agctgttgtt gtggctgctg
     1141 agggagttca tcaaacagga cctgaattaa tctgcgggtc atcggctcag cacgctgagt
     1201 cgtcgtcctg tttgccaggg ttaaaatcaa ttcccggcat aagggcgata ttttcaatgt
     1261 gcaacatgtt gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc
     1321 gttagctgtc acttgattgc tatgttccac cccgcccggt atccaaaccg catattgcgg
     1381 cggcaccatc cataaagcat tttccaccgt acaggtaatt gcaccatgta atgctagtat
     1441 cagctgtcct ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat
     1501 acaaaatgcg accgcggctt catggtgtcg gtcgggttca tagccattga gattcaacct
     1561 gtgcatcatt ttgtccgaat ttagcgataa tttgtcattt tagcttgatt caacataaca
     1621 ataaaaacgg taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact
     1681 tcattaagcg gcaaaaacag gattgtcctt atcgctggca ttctgatgat tgccacaaca
     1741 ttacgcgtca cctttatcgg cgcagcaccg ttactggata cgattcgttc cgcttactcg
     1801 ctgacgacag cgcaaaccgg cttattgacc accctgccat tattggcctt tgcgctaatc
     1861 tcacctttgg ccaccccggt agcgcgacgt tttggtatgg aacgtagcct gtttgccgcg
     1921 ttacttttga tctgtgctgg tatcgcaatt cgctctctcc cttcgcctta cttattattt
     1981 ggcggtacag cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta
     2041 attaaacgcg atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg
     2101 ggagctgcag cggcactggg atcggctatg atagtgccgc tggctttgaa cggttttggc
     2161 tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc tggctctttt tttatggctg
     2221 ccacagtggc gaagccaaca acatgcaaat ttgagtacct cgcgcgcctt acatactcgg
     2281 ggtatctggc gttcgccgct tgcctggcag gtaacattgt ttcttgggat caactcactg
     2341 atctattacg tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa
     2401 gcacaggcgg gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg
     2461 atccctcttt tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg
     2521 atgtgcgcag tgggcgctgt tgggctctgc tttatgccag cgcacgcgat cacctggact
     2581 ctgcttttcg gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt
     2641 cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga tggcacaatc cgtcgggtat
     2701 ttgctggcag cctgtgggcc gccgctaatg ggtaaaatac acgatgctaa cggtgactgg
     2761 tccgtaccac tgatgggtgt tgcaatactt tcactactga tggcgatttt cggactttgc
     2821 gccgggagag acaaagaaat tcgctaatat ccggtgctat agtgacgtga caaatcatgc
     2881 gtgaaaggga gaacaaacac gatgaatatt cagtgcaaac gcgtttatga tccggctgaa
     2941 cagagcgatg gttatcgcgt actggtcgac cgcctctggc cgcgcggtat caaaaaaacc
     3001 gatttagccc ttgatgagtg ggataaagaa atcacgccgt caacggaact gcgcaaagcc
     3061 tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc agaactggcg
     3121 caacacgagc aagaaggaaa gcggctggcg gacatcgcca aaaaacagcc gctgaccctg
     3181 ctctacgcag caaaaaacac cacgcagaac catgcgctgg tgctggccga ctggctacgt
     3241 agcttgtgat tttagtacag catccggcgg ttatttttca ccagccggat ggtcccgtcg
     3301 ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc
     3361 ttgcgctgtc gacactggaa tgagcgtccc gcccttctgc tggcaataga ccgacgccgg
     3421 atttgccata ccaatctgcg gcggtttagg tacttctggc tgagaagagg ttgaacaacc
     3481 agccaggacc agcaagcaag gcaaagcaaa actgataatt ttcatttatt gatctcactc
     3541 atttatctac gcttagagta tcgcggtatc gttttgtttt gcagcactat ttttattaca
     3601 ttcactcaaa acatattacg tcttgtttca tctttgttgg tgatgtttta tcatgcctgc
     3661 aaagattaaa taatcagcat ttacccgccg tatcctggag ttgtttcgtg tcagatcaga
     3721 ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc actggaaagt ctggtccgac
     3781 agcttctgga gatgctggaa atggtcactg atatggaatc aacctacctg accaaagtgg
     3841 atgtcgaagc gcgcctgcag catattatgt ttgcccgtaa cagccagaaa atgcacatcc
     3901 cggagaattt taccgtctcg tgggattact cgttatgcaa acgcgccatt gatgaaaact
     3961 gctttttcag cgatgaagtc cctgaccgtt ggggcgactg tattgcggca cgcaatcttg
     4021 gcatctccac atttctgagc acaccaattc acttaccgga tggatcattc tatggcacgc
     4081 tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg cgcggaacag gttttacagt
     4141 tattcgccgg actgattgca caatatattc aaaaagaggc actggttgaa cagttgcgcg
     4201 aagccaatgc tgcgctgatt gcgcaatcgt ataccgactc gttaaccggg ttgccgaatc
     4261 cgcgggcgat ttttgaaaat ctgacgacgc tgttttctct cgcccggcat cttaaccata
     4321 agataatgat cgcgtttatc gatctggata acttcaaatt aatcaatgat cgttttggtc
     4381 ataatagtgg cgatctgttt ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa
     4441 atggcgaagt tattggtcgt ctcggcggtg atgagttttt ggttgtttca ctaaacaacg
     4501 agaatgcgga tatttcgtcg ctgcgagaac gcattcagca gcaaatacgt ggagaatatc
     4561 acttaggtga tgttgatttg tattatcccg gtgccagtct tggcatagta gaagtcgatc
     4621 ctgaaacaac cgatgcagac agtgccctgc atgctgccga tattgcgatg tatcaggaga
     4681 aaaaacacaa acagaaaaca ccttttgtcg cgcattcagc gccacattgc tgaggcgtat
     4741 tcacatcctt ttgattggtg ataacatgcg aatcggtatt atttttccgg ttgtaatctt
     4801 tattacagcg gtcgtatttt tagcatggtt ttttattggc ggctatgctg ccccgggggc
     4861 ataaagatga aaaaaacaac gattattatg atgggtgtgg cgattattgt cgtactcggc
     4921 actgagctgg gatggtggta acgtcacctc taaaaaatag caaaggctgc ctgtgtgcag
     4981 cctttgtgca atttaagcgt taacttttaa tcttcctgta gataaatagc acgacaatcg
     5041 caccaataac ggcaaccacg aagctgccaa aattgaagcc atcgacttta ccaaagccaa
     5101 acagcgtgct gatccatccg ccgactacgg caccgactat ccccagcagg atagtcataa
     5161 agaatccacc accatcttta cctggcatga tccacttcgc cagaataccg gcaataagcc
     5221 caaaaataat ccatgacaga atgcccattg tttcctcact tatctgtttt gcattagcgg
     5281 gttagtcgct gataaaaagc atagcacaac atcgggaggg caagatttgt gacgagcatc
     5341 acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc
     5401 aaccacaaac atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt
     5461 ttatcacttc agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat
     5521 cgacagaaac gccattactg ttattggtga cggtcacggt atacgttgct ttgcccatga
     5581 ttcatttccc gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag
     5641 ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact
     5701 tttctggagg gaacacctca aactgccccg cttcgataag gatcacctga tcaggctctg
     5761 cactgtgttc atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg
     5821 ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc gaatattccg gcaggcgcag
     5881 tttgtttatt ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca
     5941 tccttttccc tccttcggtg aatgcgctga aaacggttta ttccagccgt ttcagggtac
     6001 gcctgataat ttgcatttta aataccattt attggttact ttttagcacc atatcagaga
     6061 aaaatcaggg aggattacaa gtgggaagcc cgcgacaacg cgggctgaat gtatcaggat
     6121 tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca aaccaatcag tgagttgcca
     6181 actttagcca gtttcttttt ggtacgtatg tactgcgtga caaaagcacc agaaataatc
     6241 aggaagctca aatagcagaa actcaccagt tccagcgtcg tcgccagaat aaagaatgaa
     6301 attcccgtat gtggggcatt aacatcgata aactgtacga aaaacgacac atagaacaaa
     6361 atggctttcg gattagtcag gctcaaaatt aacgcgcgtt taaaaatggc cccgtattgg
     6421 ggctcatcgg atttggcctc gttattttta cccttcaggg tcgcgtagag aattttactc
     6481 cccagataga gcaaataaaa cgcaccaaga taacgtacaa tgttgaataa tatcggggtg
     6541 gtcttaatta atgtcgccac tccagcccat gccagaaaca tcaatactgc atcgccaata
     6601 aatacaccgc aggccgcaag ataaccgcct ttcataccgc tactgacgct atttttgagt
     6661 acaaacaggg tatttggccc tggcaccaac acaataaaaa tggccccaac cagataggtc
     6721 cagtaattca gaaccccgta ttcagcgaac acattaacct ctttaattat ctttcgatca
     6781 tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg cttagaaagg agaaaatcag
     6841 gttttaaccc gatatcaacc cgataattga ataattaatc aggcatgctc cagtgaaaaa
     6901 ttcgggtagt gctcggcaaa atactggcgt aaaaattcta tcgttatccg cactttcgct
     6961 gacgtcgcca gccttgaaac ataaacggcc cagacgttcg ctggctggta atattccggt
     7021 agcacttgca ctaaatgacc actggcaatg ttttcgctaa catcccacca ggagcgcaga
     7081 gcaatcccct gcccatcgag acaccactga tggacaattt ctccatgatt agacgacagt
     7141 gggccggtga ctttaatcgc atggtggcct tctttattgc gtaattgcca gacaccaaaa
     7201 ggatgatcgc gctctttaat gaccaggcac ggcaatgcgg aaagatcggt taaatgtttc
     7261 ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga gaatgcggta attagtcgcc
     7321 agtttgcggg cgattaaatt aggggcgatg tcatcgccaa tgcgaatatc gagatcaaca
     7381 ccttcattga ccaaatcgac caaccggtct tccacatcaa aacgtaattc cagttgcgga
     7441 tacgccttag ccagcgctga taacgccgga gccaccacct gtcgtccaaa accaaagctg
     7501 ctgataatac gcagcatccc ctgcggcacc tgacgcacgt cagaaagttc gtccatcatc
     7561 tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa tccgctctcc ttcttcggta
     7621 atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt ttagcgtttg ctccagcaag
     7681 gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt cttccgccac ggcggcaaaa
     7741 ccagcccggc gagccaccag cataaagacg cgcaaatcgt tcagcagcgg taaattattc
     7801 atgattcgtg ttttatgttt caccagttac ggggattaat tccttttcag tcaattatag
     7861 gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa cccaatgatg aaaacgatgc
     7921 gtattgctgc gatcccggga gatgggattg gcaaagaagt ccttcctgaa gggattcgcg
     7981 tattacaggc tgccgctgag cgctggggct tcgacttgag ttttgagcaa atggagtggg
     8041 cgagctgcga gtattacagc catcacggta aaatgatgcc ggacgactgg catgagcaac
     8101 ttagccgttt cgacgccatc tattttggtg ccgtcggctg gccggatacc gttccggacc
     8161 atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga attcgaccag tacgtcaacc
     8221 tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct ggcgggaaaa cagcctggcg
     8281 acatcgattt ttacgtggtc agggaaaaca ccgaaggcga atattcctcg ctcggcggta
     8341 gagtgaatga aggtacagag catgaagtcg ccattcagga atcggtattt acgcgtcgtg
     8401 gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca aagccgccca cgtaagacgc
     8461 taacttctgc cactaaatca aacggtttag ccatcagcat gccgtactgg gatgagcgag
     8521 tggaagcaat ggccgagaat tacccggaga tccgctggga caagcagcat attgatattc
     8581 tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt ggtggtggcg tccaatttgt
     8641 ttggcgatat cctttccgat cttggcccgg cctgcaccgg caccattggc attgccccat
     8701 ccgccaacct gaatccggaa cgcactttcc cgtcgctctt cgagcctgtc cacggttccg
     8761 cgccggatat ctacgggaaa aatattgcta accctatcgc cacaatttgg gccggtgcga
     8821 tgatgctcga ttttctcggc aatggcgatg agcgtttcca gcaagcgcat aacggtattc
     8881 tggcagcgat tgaagaagtg attgctcacg ggccgaaaac accggatatg aaaggcagtg
     8941 ccaccacgcc acaggttgcc gacgcgattt gcaaaattat tttgcgttaa ggtcaaacca
     9001 gtttatttga accgcgtcac tgacgcggtt tttttattcg ttctttgcag ttaataacct
     9061 gcgtcatttc accttttatt gtttccgttt cgtgttttat ggctttccgt attcttaatt
     9121 gtttaattta tgtaacatgc aaataatcaa tttgttaaac acaacataca actcattacc
     9181 ctgcaaataa ttacaactgg caaaagccag agacgggctt ttatgcccag gagatgactc
     9241 atgatgagca atgttaagaa aaaagatgtg ccgctgataa gcatcagcct ggtggccatt
     9301 cttttcatcg cagctgcatt aagccttttc ccacaacaat cggccgacgc ggccaacaca
     9361 atatacactt ttgttacgcg tacgttaggt tccgccgtac aggtattggt tttgctggca
     9421 atgggactgg tgatttattt agccaccagt aaatacggca atattcgtct tggcgaagga
     9481 aaaccggaat acagcacgct ctcctggctg tttatgttta tttgtgccgg tttaggttct
     9541 tctacgcttt attggggggg tgctgaatgg gcctattatt atcaaacgcc tggattaaat
     9601 atcgcaccac gttcacaaca ggcactcgaa tttagcgtcc cttactcctt cttccactgg
     9661 ggcatcagca cctgggcaac ttatacgctg gcctcattaa tcatggctta tcactttcat
     9721 gtgcgtaaaa acaaaggtct gagtctttcc ggcattattg ccgcgattac cggcgttcgc
     9781 ccgcaaggcc catggggaaa actggttgat ttgatgtttc tgatcgccac tgtcggcgca
     9841 ctgactattt cccttgttgt caccgcagca acctttactc gtggactttc cgcgctgacc
     9901 ggtttacccg ataacttcac cgtgcaggca tttgtgatcc tgctttccgg cggcattttt
     9961 tgcctaagct cgtggattgg tatcaacaac ggtttgcaac gtctgagcaa aatggttggc
    10021 tggggcgcgt tcctgctgcc attactggtg ctgattgtcg gtccaaccga atttattacc
    10081 aacagcatca tcaatgccat cggcctgacc acgcaaaact tcctgcaaat gagcttattc
    10141 accgatccgc ttggcgatgg ttcatttacc cgcaactgga ccgttttcta ctggctgtgg
    10201 tggatctcat acccccctgg cgtagcaatg tttgtcaccc gcgtttcccg cggtcgtaag
    10261 attaaagaag ttatctgggg actgatcctc ggcagcaccg tcggttgctg gttcttcttc
    10321 ggcgtaatgg aaagctatgc cattcatcag tttatcaatg gcgtaatcaa cgtcccacag
    10381 gtgctggaaa cactgggcgg cgaaacagct gtacagcaag ttctgatgtc gttgccagcc
    10441 ggtaaattgt tcctcgccgc atacctgggc gtgatgatta ttttccttgc ctcgcatatg
    10501 gatgcagtgg cctacaccat ggcggcgacc agtacgcgta atctccagga aggtgacgat
    10561 cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca ctctgatccc gctttccatc
    10621 ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg tcgtgctcac agcccttccc
    10681 ttcctcgtca ttttactggt gaaagtcggc ggatttattc gctggctgaa acaggattat
    10741 gccgacattc cggctcatca agttgaacat tatctcccgc agacaccggt tgaagccctg
    10801 gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag gcgacaactg agcgccatca
    10861 tcctaacgat aaaggtatcc ctatgagcaa tctgagccct gactttgtac tacccgaaaa
    10921 tttttgcgct aacccgcaag aggcgtggac cattcctgcc cgtttttata ccgatcagaa
    10981 cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc tggatttgcg tcgctcacag
    11041 cagcgaactg gcgaatgcca atgattatgt gacgcgtgag atcattggcg aaagcatcgt
    11101 gctggtacgc ggtcgtgata aggttttgcg cgcgttctat aacgtgtgtc cgcaccgtgg
    11161 tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg attacctgcc catatcacgc
    11221 atgggcattc aaactcgatg gcaatctggc ccatgcacgt aactgcgaaa acgtcgccaa
    11281 tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg gaggaatatg ccggattcgt
    11341 cttcatcaac atggacccca acgccaccag cgtagaagat cagttacccg gtctgggcgc
    11401 gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa ctggcggccc gctttaccac
    11461 ccgcacgcct gccaactgga agaacattgt cgataactat ctcgagtgct atcactgtgg
    11521 tccggcgcat ccaggtttct ccgactccgt acaggttgat cgctactggc acaccatgca
    11581 cggtaactgg acgctgcaat acggtttcgc caaaccgtcc gaacagtcgt ttaaatttga
    11641 agagggtacg gatgcggcat tccacggttt ctggctgtgg ccgtgcacga tgctgaacgt
    11701 caccccgatc aaagggatga tgacggtcat ttatgaattc ccggtggatt ctgaaactac
    11761 cctgcaaaac tacgatattt acttcaccaa tgaagagtta accgacgagc aaaaatcgct
    11821 gattgagtgg tatcgcgatg tgttccgtcc ggaagattta cgtctggttg aaagcgtaca
    11881 gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc atcatggccg acagtagcgg
    11941 tagtggcatt tccgaacatg gtatcgccca tttccataat ctgctggcgc aggtgtttaa
    12001 ggactaatga catcggcggc ggtattttcc gccgctgggc tgatttttga tggagtacag
    12061 caatgtcaga ctatcaaatg tttgaagtac aggtgagtca ggtggaaccg cttaccgaac
    12121 aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc gctgcctgcg ttcaccggag
    12181 gaagtcacat cattgtgcag atgagcgatg gtgataacca gtacagcaat gcgtattcac
    12241 tactgagttc gccgcatgac acctcttgtt atcagattgc cgttcggctg gaggaaaact
    12301 cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt gggcgatcgg ttaacgattt
    12361 caacgcctaa taacctgttt gcgctaattc cctcagccag aaagcatctg tttatcgcgg
    12421 gcggtattgg tatcacccct ttcctgtcgc acatggcaga gttgcaacac agcgacgtcg
    12481 actggcagct acattactgc tcgcgaaatc cagaaagttg cgcatttcgt gatgagctag
    12541 tccagcatcc gcaggctgag aaagtccatt tgcatcattc gtcaaccgga acacgactgg
    12601 aattagcgcg attattggcg gatatcgaac ctggcacaca cgtttatacc tgtggccccg
    12661 aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct ggacatcgcc gccgatacgc
    12721 tgcactttga gcaatttgct atcgaagaca aaaccggcga tgcctttacc ctggtgcttg
    12781 cccgttccgg aaaagagttt gtggtgccgg aagagatgac tattttgcag gttattgaaa
    12841 ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg ggtatgcgga acctgcgaaa
    12901 cagcaatact ggaaggtgaa gctgaccatc gggatcagta ttttagcgat gaagagcgtg
    12961 ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg
    13021 atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc ccagtccggc ttcggaagat
    13081 tttactgcgg atattcctgc aataaattgt gtaatgcttc cgccatcagc tcaccacgcc
    13141 agccggaaat cagctccggc aaattgttct gcggtttcag tttccagtgc cagttcagca
    13201 gttggttgat ttgccgacgc gatgccagca attcggcgct gatcttatgc gtttcgctca
    13261 cgtcagtaat cagcgactta atcgctttaa acgctttacg ataacccggc atgtccatca
    13321 ggttaagcat cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg
    13381 ccagcagcgt tttaccgtga aagcggattt cgctaccgga taaacccagg ctgtccagtt
    13441 cgcctaaact tcccggcata taacgcgcga ccgaccacaa atgctcttca cgcacgacaa
    13501 agttcaccgc cagatcacgc tcacgagcct tgcgcagtcg ccagtcggct aacagttgca
    13561 gacaggccag ttggcgtgtg cgtaattgcc aggcattggt gatatcacgc caggcatctt
    13621 ccggcgcaac gacttcctga cgacgcattt gcatcaggcg gcattcatcc agcgccgcag
    13681 gtagccagcc ggaggcctcc gtttctacca taagcttggc ggtgatcggt aacagatacc
    13741 agacatccgc cgctgcgtat tcacactgac gttcggtcag cggtctggcc agccagtcgg
    13801 tgcgcgattc actcttgtcc agcgtaacgc cggaatactc ttccaccatg gaagcgaaac
    13861 cccatgacat cgggcgtccg cagaaggcag caaggatttg cgtgtcaatc aggggttgtg
    13921 gtaattcgcc aaagacattg aggaacactt ccagatcttc actgcctgca tggagaaatt
    13981 ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg atcccgagag
    14041 gatcaattag cgccagatgc tcaccatcga aaagttgaat caaccccagc tgcgggtaat
    14101 aagtgcgcgt acgaacaaat tcagtatcct gggctatcgc cggaaagaca cggacggctt
    14161 cacacaaaga agccagcgca tcgtccgtgg taatcatttg gtaattcaaa ttgtattctc
    14221 tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga cttaacgctc
    14281 aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc
    14341 cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact ttgtagcccg
    14401 tgagctgacg gcagcaaaag gtcaccagtg actcttcggt aagcgatgga tcttttttca
    14461 ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca gccgcgactt
    14521 cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac
    14581 cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaaccct tcttcatcca
    14641 tcaccgcgat gtcgccggtg tgtaaccagc catttttgat gatctcatcg gtagcatcgg
    14701 gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca cccggttgac
    14761 ccggtggtac ttcattatcg tcgtcatcca ccagtttggc ttccgtcgac ggcaccggca
    14821 aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc agcggcgcac
    14881 actcggtaag accatagcct tccagcagat actgtcctgt cagtttcacc caacgctctg
    14941 ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg gagaaatcca
    15001 gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg ccagtgatag
    15061 cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcgcgc gggttagtga
    15121 taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc agggcaaaaa
    15181 tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc aacggcccat
    15241 aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt
    15301 tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc ggcaccagtt
    15361 cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat gaaatggcat
    15421 ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa ttgactaccg
    15481 tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc tgaacggcgg
    15541 ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca atcgccgatg
    15601 cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg ttaacgttta
    15661 cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc aataaattag
    15721 gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa cccttgttgc aaataagcgg
    15781 caaacgcgcg actgcgttct tccagcttgc ggaaggtcat tacctccccc atattcacaa
    15841 acgcaggctg atcggcgtag cggacgaccg actgctcaaa catatctatc agagattgat
    15901 aacggtcagg gttgatctcc gtcggaacat ccgcgggata acggttaagc caaaccttct
    15961 tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaagt gataaacatg
    16021 ttgttaacat aacattaact catcttacca gcttgataat tagacaacga aaaggttgcg
    16081 aagcgcgtca ctatttattt ttatagttgc cgtatgaacg cagaaacagc ggaccagccg
    16141 ctgtttcttt ttctttaaaa acaacttgtt actcggttac aattgtttca actcttgcag
    16201 ggccaggatt ataccaaccc catccgccgt aaccataggg ccagccgcgt ccaccataaa
    16261 accatggatc aattggctgc ggcggcataa tcacctgttg agtgatatgc cagcgtttgt
    16321 aacctgttac ctgcataacc ataaatttat atggtgcatt atttactttg ccatccactg
    16381 tgccagtgat tggcccgaca accgtcacca gttgtccacg aaaatcgacc ggatcaagaa
    16441 aaccgttcac atcggcataa atgcgcccgc gtgaaggttc tcccaaggtt ggtcttgctc
    16501 cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtacca
    16561 caaccacttt gccgccaaag cgcgcctcct gaccaacata taaatctggc gcattcatca
    16621 cgcgtaccag atcttgctgt ggtgttggac tgctgccttt aatggcgtcc ggcacagtga
    16681 cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt
    16741 gaaccgccat gatgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc
    16801 ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca tgttccaccg
    16861 ccaccgtttt accctcggca aacatctgac acgcaatcgg cagcatatct tccgccgctg
    16921 gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttacggaga tccggccagg
    16981 cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt cgttcatgaa
    17041 cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg ttttcatcac
    17101 gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc aatggctgcc agcacgcggg
    17161 ttgcgccgtt tttgcgccac gccccctgcg ccatcgtcat tagcgtagag acaccaatca
    17221 tcggtaactc cgcgccaagc gccagccctt gcgcaatacc aataccaatg cgcacgccag
    17281 taaagctacc ggggccgcgc ccgtaagcca gagcgtttat gtcagtcagg gaagttccgc
    17341 tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca cgagggcaaa
    17401 gctcaaaatg agcgttgaca ttaccgtcgt tccacagagc gacagagcag acctctgtcg
    17461 cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca
    17521 tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac
    17581 gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag
    17641 gacgcatcac cagccgattg tcacaaatta ccagcacgcc gcgatcgtcg gcgtcgcgaa
    17701 tcagtcgccc tactccctgc ttgagagtaa tgaccgcatc cggcagctgc acttcatcga
    17761 acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc ttttaacagt agatcatccg
    17821 gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa tgtatcgcca cgcacgtcca
    17881 ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg acaaattgct
    17941 gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc atggtagcgc
    18001 ggaactgctc ggccagatcg cgcatcatgg catgcgaggt acaaagcata aaacaacgac
    18061 cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag ttgccgtgca caacccggtt
    18121 gattggtttg cggcaggttg cgcggcacac agagtaacgc ctggcggctg taatcaaacg
    18181 ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca
    18241 gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccgggc ttttgcgcca
    18301 ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg
    18361 aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc
    18421 gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag caaggcggaa cgccccagtg
    18481 acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa aatgcccgct
    18541 gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc tcaccgagtt
    18601 gcagacgaaa atcctgtgca ctctgggcaa gacgatcagc gcacttttgt aactgctggg
    18661 tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc
    18721 gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt
    18781 cgaagatcat gacgtccgct tcagggatca actcgccaaa tccactctct ttaaccacca
    18841 tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac
    18901 gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt
    18961 tggtgctggt gaccagcggc cacgcctgtg aatcttccgc cacgctgacg caggtgctga
    19021 tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaagattt
    19081 gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt
    19141 ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt gagactgttg
    19201 gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag ataatgactt
    19261 tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta ccggttcctg
    19321 cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc gccatctgtc
    19381 gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa
    19441 aatcgtccgt cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc
    19501 tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc agcacgaaaa
    19561 tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga agcccgctgg
    19621 tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac
    19681 gccgatgcct ttgagcaaac cgccaacacg ctggcacaga ttgacgccgt gctggaaaaa
    19741 cagggcagca ataaatcgag cattctggat gccaccattt tcctggccga taaaaacgac
    19801 ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg cgggtcatgc gccagtgcgc
    19861 tgcacggtac aagcgggttt gatgaacccg aagtataaag ttgaaattaa gattgtggct
    19921 gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc
    19981 ggttttacat aaatcgacca gaga
//
LOCUS       DAZOXR010000068        19756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZOXR010000068 DAZOXR010000000
VERSION     DAZOXR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.070"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            215..985
                     /locus_tag="ACHUNN_002114"
     CDS             215..985
                     /locus_tag="ACHUNN_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0576487.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            976..1449
                     /locus_tag="ACHUNN_002115"
     CDS             976..1449
                     /locus_tag="ACHUNN_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0576488.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1557..2096
                     /gene="dnaT"
                     /locus_tag="ACHUNN_002116"
     CDS             1557..2096
                     /gene="dnaT"
                     /locus_tag="ACHUNN_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0576489.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2099..2836
                     /gene="dnaC"
                     /locus_tag="ACHUNN_002117"
     CDS             2099..2836
                     /gene="dnaC"
                     /locus_tag="ACHUNN_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0576490.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2885..3379
                     /gene="yjjA"
                     /locus_tag="ACHUNN_002118"
     CDS             2885..3379
                     /gene="yjjA"
                     /locus_tag="ACHUNN_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0576491.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3633..5924
                     /gene="opgB"
                     /locus_tag="ACHUNN_002119"
     CDS             3633..5924
                     /gene="opgB"
                     /locus_tag="ACHUNN_002119"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0576492.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6063..7085)
                     /gene="lgoD"
                     /locus_tag="ACHUNN_002120"
     CDS             complement(6063..7085)
                     /gene="lgoD"
                     /locus_tag="ACHUNN_002120"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR0576493.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7224..8138
                     /locus_tag="ACHUNN_002121"
     CDS             7224..8138
                     /locus_tag="ACHUNN_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0576494.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8353..9704
                     /locus_tag="ACHUNN_002122"
                     /pseudo
     CDS             8353..9704
                     /locus_tag="ACHUNN_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9753..11408)
                     /gene="tsr"
                     /locus_tag="ACHUNN_002123"
                     /pseudo
     CDS             complement(9753..11408)
                     /gene="tsr"
                     /locus_tag="ACHUNN_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11527..11973)
                     /gene="hpaR"
                     /locus_tag="ACHUNN_002124"
     CDS             complement(11527..11973)
                     /gene="hpaR"
                     /locus_tag="ACHUNN_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0576495.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12245..13534
                     /gene="hpaG"
                     /locus_tag="ACHUNN_002125"
     CDS             12245..13534
                     /gene="hpaG"
                     /locus_tag="ACHUNN_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0576496.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            13531..14997
                     /gene="hpaE"
                     /locus_tag="ACHUNN_002126"
     CDS             13531..14997
                     /gene="hpaE"
                     /locus_tag="ACHUNN_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0576497.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            14999..15850
                     /gene="hpaD"
                     /locus_tag="ACHUNN_002127"
     CDS             14999..15850
                     /gene="hpaD"
                     /locus_tag="ACHUNN_002127"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0576498.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15860..16240
                     /locus_tag="ACHUNN_002128"
     CDS             15860..16240
                     /locus_tag="ACHUNN_002128"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0576499.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16351..17154
                     /gene="hpaH"
                     /locus_tag="ACHUNN_002129"
     CDS             16351..17154
                     /gene="hpaH"
                     /locus_tag="ACHUNN_002129"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0576500.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17165..17953
                     /gene="hpaI"
                     /locus_tag="ACHUNN_002130"
     CDS             17165..17953
                     /gene="hpaI"
                     /locus_tag="ACHUNN_002130"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0576501.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18128..19504
                     /gene="hpaX"
                     /locus_tag="ACHUNN_002131"
     CDS             18128..19504
                     /gene="hpaX"
                     /locus_tag="ACHUNN_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0576502.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19514..>19756
                     /locus_tag="ACHUNN_002132"
     CDS             19514..>19756
                     /locus_tag="ACHUNN_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0576503.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4706 a         5335 c         5107 g         4608 t
ORIGIN      
        1 gccaaacaat aaattcatta tagtgataat attcatgtga tagtttgttt gcggcgagag
       61 ataattcgct ttttatcacc gagcttgtcc cgataaccag gattaatcag cgcaattttg
      121 atcttaacct accgcaaaac aaccgtgctt catgatacaa atggattctc attatctgaa
      181 caatacgcag cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt
      241 aacacggctt tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt
      301 ggttgatgag ctttcctcac gactgggtcg ggcgttgggg atggacagcg tcgaaagttc
      361 tatctcttcg aacgccatag tgctgacaac cattaaagat gggcaatgcc tgacatcgac
      421 acgtaaaaat cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt
      481 gattctggcg gagcaccatc tgctggatta caaaggcgta gagaaacgat ttagccaaat
      541 tcagccatta cgttacccaa gatggctggt agccttaatg gttggccttt cttgcgcctg
      601 tttctgcaaa ctcaataaag gtggctggga tggtgccgtc atcaccttct ttgccagtac
      661 ggccgcgatg tatatccgcc agctgctggc acaacgtcat cttcatccgc agatcaactt
      721 ttgcctcacc gctttcgccg ccaccaccat ttccggattg cttttgcaac ttcccacttt
      781 cagcaataca cccactatcg cgatggccgc cagcgttctg ctgctggtgc cgggatttcc
      841 gttgattaat gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg
      901 ggcgatcgcc agtctgctga cactggctac ctgcgtcggc gtggtgatgg cactgacgat
      961 ttgggggcta cgcggatggg tgtgatcgag tttctgtttg cattggcgca ggatatgatc
     1021 ctcgccgcca ttccagcggt cggctttgcg atggtgttca acgttcccgt tcgggcatta
     1081 cgctggtgtg cgctgcttgg cgcgattggt catggttcac gaatgatctt aatgaccagc
     1141 gggctgaata ttgagtggtc aacctttatg gcctccatgc tggtcggcac cattggtatt
     1201 caatggtcgc gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct
     1261 atgttcccgg gcatatcggc ttataccgca atgatttcgg cggtaaaaat cagccagtta
     1321 ggttacagcg aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt
     1381 gttggtgcgt tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct
     1441 cgcgtataaa attgcctcgc ttaaagcaca cggatgagag acagcctcct ctcctccgtg
     1501 tgttactata aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt
     1561 cttccagagt tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa
     1621 ccgttctggc aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt
     1681 tttatgccgt cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctggcgc
     1741 gtccgggaag cgatgtcgct ctggacgatc aactctatca ggaaccgcaa gccgctcccg
     1801 ttgctgtacc catggggaaa ttcgccatgt atccggactg gcaacccgat gccgatttta
     1861 tccgcctggc ggcgctatgg ggtgtggcac taagagagcc ggtgaccacc gaagaactgg
     1921 cctcattcat tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac
     1981 aaaaactggc gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag
     2041 atgtgaatac ggtcagcgaa cctgacagtc aaattccacc aggattcaga gggtaacgat
     2101 gaaaaacgtt ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc
     2161 tgcattcaaa acgggtgaag aactactggc ctggcagaaa gaacaaggag cgatccgctc
     2221 cgccgctctc gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg
     2281 tattcggcca ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca
     2341 gatgaatgcg ttaagcaaag cgcgccagta cgtcgaagag tttgacggca acatcgccag
     2401 cttcatcttt tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa
     2461 cgagctgctg ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc
     2521 gatgaaagat accttcagga atagcggtac cagtgaagaa caactgctta acgatctgag
     2581 caacgttgat ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa
     2641 agtgatcatc aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct
     2701 gaccaacagt aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat
     2761 gcgcctgggt aacagtttgt gggtgatctt caactgggat agctaccgta gccgggtaac
     2821 aggtaaagag tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt
     2881 aatgatgatg aaaactgtta aacatcttct gtgctgtgcc attgccgcca gcgcattaat
     2941 ttccaccggg gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg ccagcgaact
     3001 tggcaaccaa aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt
     3061 gcttagcagc gggaaccagg ccttaagcgc agataacatg aacaacgccg caggcattct
     3121 gcaatactgc gcgaagcaaa agctggcgtc ggtaaccgat gccgaaaaca tcaagaacca
     3181 ggtgctggaa aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga
     3241 tggcattcag ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg
     3301 aacgactccg ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg
     3361 gctgaacttc atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg
     3421 gctctctcct gcccttttcc ctgctcctgg acggttttcc ccctgttaca cgggaagcga
     3481 ccgcgatcaa aaaagtctgt ttataaacta attcctaaca acatcgcatt tttttgacac
     3541 tagaattgca gcaatatggc gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg
     3601 gctaaacggc ccggtttagt gaggatcgcc cgttgtcaga actactctct ttcgcccttt
     3661 ttctcgcctc tgtgctgatt tacgcatgga aagcgggacg taacacctgg tggtttgcag
     3721 ccacattaac ggtgctgggg ctatttgtcg ttttaaatat caccctgttt gccagcgact
     3781 attttactgg cgatggtatt aacgacgcgg ttctctatac cttaaccaac agcctgaccg
     3841 gtgctggcgt cagcaaatac atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg
     3901 tgttcggtgc gctgggctgg atcctgcgcc gtcgtcgcca tcatccgcac cattttggtt
     3961 acagcctgct ggcgctctta ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc
     4021 agataacgga actggtgaaa tcccagtcac gcgatggcga cccggacttt gcggcttatt
     4081 ataaagagcc gtcgaaaact atccctgacc cgaaactcaa cctggtttat atctacggcg
     4141 aaagtctcga gcggacctat tttgataacg aagctttccc ggatctcacg cctgaactgg
     4201 gcgcgttgaa aaatgaaggc ctggatttca gccacacgca gcagctgcca ggaacggatt
     4261 acacgattgc gggcatggtg gcttctcagt gcggcatacc gctgtttgcc ccctttgaag
     4321 gcaacgcctc cgcctctgtc tccagcttct tcccgcagaa catctgtctg ggcgatatcc
     4381 tgaaaaactc gggttatcag aactatttcg tgcagggcgc gaatctgcgt tttgccggta
     4441 aagatgtgtt cctgaagtcg catggcttcg accacttata cggctcagaa gagctgaaaa
     4501 gcgtggtggc cgacccgcac tatcgcaacg actggggatt ctacgacgat accgttctcg
     4561 atgaagcgtg gaaaaagttt gaagagcttt cccgctcagg tcagcgattc tcactgttta
     4621 ccctgacagt cgatacccat cacccggatg gttttatctc gcgtacctgt aaccgcaaaa
     4681 aatatgattt tgacggtaag ccgaaccagt cattcagcgc ggtaagttgc agccaggaga
     4741 atatcgcggc gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg
     4801 tcgtctcttc tgaccattta gcgatgaaca acacggcgtg gaaatacctc aataagcagg
     4861 atcgcaataa cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag acgctggcag
     4921 tgaagcgtaa cacgatggat aacggcgcga cggtgctgga cattctcggt ggcgataact
     4981 atctcggact tggtcgtagc agtttatccg ggcagtcgat gtcggaaatc ttcctcaata
     5041 tcaaagagaa aacattggcg tggaagccgg atatcatccg cctgtggaaa ttccctaaag
     5101 agatgaaaga gttcaccatc gaccagcaga aaaacatgat tgccttctcg ggtagccatt
     5161 tccgtttgcc gctgctgttg cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg
     5221 aatactcagc accgctgcgt ttccagctgg ccgatttcgc tccacgcgac aatttcgtct
     5281 gggttgaccg ttgctataag atggcacaac tctgggctcc ggaactggca ctctccaccg
     5341 actggtgtgt ctcgcaaggg cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa
     5401 caacatggca gggcaaaacg gcatttaaag atacggtcat cgacatggcg cgttataaag
     5461 gcaatgtcga tacgctgaaa attgttgata acgatattcg ctacaaagcc gacagtttca
     5521 tcttcaacgt cgccggtgcg ccagaagagg tgaaacagtt tagtgggatt tcccgtccag
     5581 agtcgtgggg ccgctggtcc aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc
     5641 atccgctgcc aaagaaattt gacctggtga ttaccgccaa agcatacggc aataacgcca
     5701 gccgtcctat tccggtacgc gtaggcaatg aagaacaaac ccttgtgctg ggcaatgaag
     5761 tgaccaccac cacactgcat ttcgataacc caaccgatgc cgacacactg gtaattgtgc
     5821 cgccggaacc tgtctcaacc aacgaaggga atatcctcgg acactcgccg cgtaagctcg
     5881 ggatcggcat ggtggaaatt aaagtggtgg aacgtgaagg ataattcctg actgatgtgt
     5941 aggtcgcata aggtgcgacc tacacacctt ctgcggccta caaaacatca ttgagaaaca
     6001 gaaaaaaaca gcgcccaaca cttccccgga cgctgcatgg caactttaat aatctcgcct
     6061 tatcagaaag taattacacc cttaatcaac tcacgattgt taatcacatc gcgctcgtag
     6121 gtttctgcca gcgtggcgaa cggatagcga tgggttaaca tcatgtcagc agtgattttt
     6181 ccttccgcca tcagtcgacc gactttagca aaatcttccg gcgtggcgtt gcggctaccc
     6241 atcatcgtcg tttctttttt atggaattcc ggatcggaga actgcaactc acctttaaac
     6301 aggccgacaa ataccaccgt gccgccgtga cgaatcaaat tcacggtgtt attcatcgca
     6361 tgttgattac ctgtcgcgtc gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgt
     6421 agctgcgcgt caaaatcttc ggctgacggg tccagtaaag gtaattccag acgcgttgcc
     6481 acatgttcac ggcgcgccgg actggtatcc gccaccacca cctgtgcgcc atcggcttta
     6541 gcgattgccg ccgcgcccag accgattggc cccgccccga ccaccagcac ctgctcgccg
     6601 ggagcaatgg ctgcgcgacg caccgcatga gcgctaatag cgaaaggttc aatcaatgct
     6661 gccgcctgcg ggtcaatacc gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac
     6721 tcactaaaac cgccatcctg atgcacgcca atgactgaaa ttttttcaca gcaattggta
     6781 cgcccgcttt tacacgccgg gcattgctga caggcaacat aagggatcac agcaacttgc
     6841 tgaccatttt taagatcagc aatattttta cccagcccaa caatctcccc acatatttca
     6901 tggcctaaaa cacgtggata actaaaaaat ggttgatttc caccccaggc atgaatatcg
     6961 gtaccgcaaa tcccgacaga cttaattttt attaatgctt cattgtcacc cggaataggt
     7021 atctcgcgtt gtttccagac taattctttc ggctgctggc aaattaaaac attcatcgta
     7081 gacatgattc ggctctccat tttttgttgc cttagatatc aatgaaaatg aaaaatggca
     7141 gcggaacgga aaatctgttt tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt
     7201 aatggactca caaggaggaa aatatgagtc gttcacaaaa tttacgccac aatgtgatta
     7261 accaggtcat tgatgatatg gcgcggggtc atatcccttc cccacttcca tcgcaaagtg
     7321 cgctggcgga aatgtacaac atcagccgca ccacagtgcg tcatattctc agccacttac
     7381 gcgaatgcgg cgtcttgacg caggttggaa acgactatgt tattgcgcgt aaacctgacc
     7441 atgatgacgg tttcgcctgt actaccgctt caatgagtga acaaaacaaa gtgtttgagc
     7501 aggctttttt cacgatgata aaccagcgcc agttacgccc aggggaaacg ttttctgaac
     7561 tgcaactggc tcgggccgcg ggtgtcagcc cggttgtagt aagagaatat cttttaaaat
     7621 tcggccgtta taatcttatt cacagcgaaa agcgcggcca gtggagcatg aagcaattcg
     7681 atcaatccta cgccgagcag ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc
     7741 agcactttct caacctgcct gatcatgatc cccgctggct gcaggcgaaa accatgctcg
     7801 aacgtcatcg gctactgcgt gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg
     7861 acagagattt tcactcctta ctgctttcag ctgccgacaa tatctttttt gatcaatcgc
     7921 ttgagatcat ctcagtcatc tttcattttc actatcaatg ggacgaaagc gatcttaaac
     7981 agcgcaatat cattgccgtc gatgagcata tgaccattct cagcgctctg atttgccgca
     8041 gtgaccttga tgccaccctt gcgctccgta accatttgaa ttcagctaaa caatcaatga
     8101 ttcgatccat taatgagaac acgcgttatg ctcattaagt acggattaaa aacagcaaac
     8161 tattagcggg tgcacggttt tatcaaaaga taaataccgg gaaattcccg cttacctatg
     8221 ctcacaatca gtcgactaaa aatgtttttg tgagcggtag taaagtccta aaactttaac
     8281 ctgccgttaa tgtcaacctt gcgtaattca caattacgta aacaattaaa attataaata
     8341 caggagtccg gcgtggaaaa agaaaatatc accctcgatc cgcgttcttc atttactcca
     8401 tcttcgtcgg cagatattcc cgtgccacca gatggattag ttcaacgcag tacccgaatt
     8461 aaacgcattc aaaccaccgc catgttgtta ttattttttg cggcggtaat caattatctc
     8521 gaccgcagtt cgctgtcggt agcaaattta acgattcgtg aagaattggg attaagtgcc
     8581 accgaaatcg gcgctttgct ctccgtgttt tcactcgctt acgggattgc gcaacttcct
     8641 tgcggcccac tattggatcg taaaggccca cgcctgatgc tgggactggg gatgttcttc
     8701 tggtcactgt tccaggcaat gtctggcatg gtgcacaact ttacgcagtt cgtgttggtg
     8761 cgtatcggta tggggattgg tgaagcgccg atgaacccat gcggtgtaaa agtcattaac
     8821 gactggttca acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc agcttctacc
     8881 attggcgttg ccgtaagccc accgattctg gcggcgatga tgctggtgat gggctggcgc
     8941 gggatgttta ttaccattgg tgtactgggg atttttctcg ccatcggctg gtatatgctc
     9001 tatcgcaacc gcgagcacgt agaactgact gccgttgaac aagcttatct caatgcaggt
     9061 agcgtcaatg cccgccgaga tccgctcagt tttgccgaat ggcgcagcct gttccgtaac
     9121 cgtacaatgt ggggaatgat gctcggattc agtggcatca actacactgc gtggctgtat
     9181 ctggcctggc ttcctggtta cctgcaaaca gcctataacc tggatttaaa aagcacaggg
     9241 ttgatggcgg ctatcccttt cctgtttggg gctgccggga tgctggtcaa cggttacgtt
     9301 actgactggc tggtcaaagg gggaatggct ccgattaaaa gccgtaagat ctgcattatt
     9361 gccgggatgt tctgttctgc cgcctttacg ctggtcgtac cgcaagcgac aacatccatg
     9421 acagcggttc tgctgattgg catggcactg ttctgtattc aacatcctgc tggggcttga
     9481 tccacgtcgc agttgcttct cgcatgactg cgtcggtggg cagtatccag aactttgcca
     9541 gcttcatctg cgcctctttt gcgccgatca ttactggttt tattgttgat accacccact
     9601 cattccgtct ggcactaatc atctgcggtt gcgtcaccgc agcgggggca ctggcgtaca
     9661 tcttcctggt tcgtcagccg atcaacgacc cacgtaaaga ttaatctcct tatgcccgat
     9721 aacattttgc ttatcgggca ttttcatggc gattaaaatg tttcccagtt ctcctcgcta
     9781 tctgccacgg ccattttacg cggcgcagct ggcgtcacgg tttttaccac agccgatgtt
     9841 tcacgctgct gttgctgaat ccggaacact gccactgctt cggtcaggcg actggcctgc
     9901 tcttccagcg cggcggcggc agcggcagac tcttccacca gcgcggcgtt ctgttgagtt
     9961 acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca
    10021 gaagcagaag caatttcgcc cataatgtcc gtcacgcggg tcacggcgct gacaatctcc
    10081 gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt ccccaccgag
    10141 tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tgcgctgggc cagattacgc
    10201 acttctcccg cgaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc agcctcaacc
    10261 gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata
    10321 tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac gttatctacc
    10381 actttgccgc cgcgttgcgc cgtttcagaa gcacttaacg ccagatggct ggcctggcgc
    10441 gcattttcgg cgttctgctt caccgttgcg gtcagttact ccatgctggc tgccgtctct
    10501 tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtggcgatt
    10561 tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc
    10621 agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc
    10681 acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc aatcaggcga
    10741 ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat gaccgccagt
    10801 acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc attgttatcg
    10861 ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg
    10921 aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt gcctgcacct
    10981 aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct
    11041 gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc cgcccagttt
    11101 ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac ggttgaaccg
    11161 ctaccaatat tgttctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc
    11221 gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt
    11281 aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc
    11341 agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat cttgatacgt
    11401 tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg aaagcaaaaa
    11461 ctttatgaaa aggtgtaacc caccatccat ggaaaaacca ggcgctggtt atcagatact
    11521 aaaaagttat tcattatcgc ccgggatggc ttcttgtcgg gaattgccca gagcaataaa
    11581 ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag cttcaatctg
    11641 ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc cctctttggt
    11701 tagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca ctaaaccgtc
    11761 gcgttccatc cgcgtgagga tcccggtcag gcttgggcgc aaaatgcagg cgcgatacgc
    11821 cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc gccactgttg
    11881 ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg cttctcgcgc
    11941 ctggagcaac gcaatggtta gtgagtcgtg cattatcttt cccctgaaaa attcaaaatc
    12001 attaatatag aaacagttaa tcgtcacgtt accgggactg cgctgcccct gcaattcctg
    12061 attttatcaa aattgatgat attttattaa cttactgata tttaatattt tattatcttt
    12121 aaatgtaaat agtttgttaa ttagatcaca tttacatcac ttacttttgc caaactacgc
    12181 aatcgggaat aaaagcaatc attaacatat taatgattaa gatgatcgaa tccgaggagt
    12241 ggtaatgaaa ggcactatct tcgccgtagc gttgaaccat cgcagccaac ttgatgcatg
    12301 gcaggaagcg ttccagcaat ccccctacaa agccccgcct aaaactgcgg tctggtttat
    12361 taaaccgcgc aatacggtga ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa
    12421 ggtactgagc ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga aagtacgtga
    12481 agaagatgcg gcagagtaca tcgccggata tgcgctggct aacgacgtca gcctgccgga
    12541 agagagcttt taccgcccgg caatcaaagc aaaatgtcgt gatggattct gccccattgg
    12601 cgaaaccgtg gctctcagca atgtcgataa tctgaccatc tataccgaga tcaacgggcg
    12661 tcctgccgat cactggaata ccgccgattt acaacgtaac gccgcgcagt tgctgagcgc
    12721 cctgagcgaa tttgccacgc tgaatccagg cgacgccatt ctgctcggca cgccacaggc
    12781 gcgcgtggaa atacagccag gcgatcgcgt tcgtgttctc gcagaaggtt tcccgccgct
    12841 ggaaaatccg gtagtggacg aacgtgaagt gaccacgcac aagagcttcc caacgcagcc
    12901 acacccgcac ggcacgctgt ttgccctcgg cctgaactac gccgaccacg ccagcgaact
    12961 ggaatttaag ccaccggaag aaccgctggt gttcctgaaa gcgccaaata ccctcactgg
    13021 cgataaccag acctccgtgc gtccaaacaa tattgaatac atgcactatg aagcggagct
    13081 ggtggtagtt attggcaaac aggcgcgtaa cgtcagcgaa gccgatgcca tggattatgt
    13141 cgcgggctac accgtgtgta acgactacgc cattcgtgac tatctggaaa actactaccg
    13201 ccctaacctg cgggtaaaaa gccgcgacgg actgacgccg atgctttcaa ccatcgtgcc
    13261 gaaagaggcg atcccggacc cgcataatct gacccttcgc accttcgtca acggcgagtt
    13321 acgccagcaa ggcaccaccg ccgatctgat cttcagcgtg cccttcctga tcgcctactt
    13381 aagtgaattt atgaccctga atccgggcga catgatcgcc accggcacac ccaaaggctt
    13441 atctgacgtg gtgcctggcg atgaagtagt ggtggaagta gaaggcgtgg gccgcctggt
    13501 gaaccgaatt gtgagtgagg atacagcgaa atgaaaaaag taaatcattg gatcaacggc
    13561 aaaaatgttg caggtaacga ctacttccag accaccaatc cggcaacggg tgaagtgctg
    13621 gcggatgtgg cctctggcgg tgaagcggag atcaatcagg cggtagcggc agcgaaagag
    13681 gcgttcccga aatgggccaa tctgccgatg aaagagcgtg cgcgcctgat gcgccgtctg
    13741 ggcgatctga tcgaccagaa cgtgccagag atcgccgcga tggaaaccgc ggacaccggc
    13801 ctgccgatcc atcagaccaa aaatgtgttg atcccacgcg cttcccacaa ctttgaattt
    13861 ttcgcggaag tctgccagca gatgaacggc aagacctatc cggttgacga caagatgctc
    13921 aactacacgc tggtgcagcc ggtgggcgtt tgtgcgctgg tatcgccgtg gaacgtaccg
    13981 tttatgaccg ccacatggaa ggtcgcgccg tgtctggcgc tgggcaatac cgcggtactg
    14041 aaaatgtcgg aactctcccc gctgaccgct gacagcctgg gtgagctggc gctggaagcc
    14101 gatattccgg caggcgtgct gaacgtggta cagggctacg gcgcaaccgc aggggatgcg
    14161 ctggttcgtc atcatgacgt acgtgccgtg tcgttcaccg gcggtacggc caccgggcgc
    14221 aacatcatga aaaacgccgg gctgaaaaaa tactccatgg aactgggcgg taaatcgccg
    14281 gtgctgattt ttgaagatgc cgatattgaa cgcgcgctgg acgccgccct gttcaccatc
    14341 ttctcgatca acggcgaacg ctgcaccgcc ggttcgcgca tctttattca gcaaagcatc
    14401 tacccggaat tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg
    14461 aacgatccga atacccaggt tggcgcgctt attagccagc agcactggga aaaagtctcc
    14521 ggctatatcc gtctcggcat tgaagaaggc gcaaccctgc tggcgggcgg cccggataaa
    14581 ccgtccgacc tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac cgtgctggca
    14641 gacgttgata accgtatgcg agtcgcccag gaagagattt tcgggccggt cgcctgcctg
    14701 ctgccgttta aagacgaagc cgaaggctta cgtctggcaa acgacgtgga gtacggcctc
    14761 gcgtcgtaca tctggacaca ggatgtcagc aaagtgttac gcctggcgcg tggcattgaa
    14821 gctggcatgg tgttcgtcaa cacccagaac gtgcgtgacc tgcgccagcc atttggcggc
    14881 gtaaaagcct ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa
    14941 atgaagaacg tctgcatttc catgggcgac catccaattc cgaaatgggg agtctgatat
    15001 gggtaagtta gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc actctgaact
    15061 gccagggaaa aaccacggtt gccgccaggg cgcgatcgac gggcataaag agatcagcaa
    15121 gcgttgccgg gaaatgggcg tcgataccat tatcgttttc gatacccact ggctggtcaa
    15181 cagtgcttat cacatcaact gtgcagacca ttttgaaggc gtctacacca gcaacgagct
    15241 gccgcatttt atccgcgata tgacctacaa ctacgagggc aacccggagt tggggcagct
    15301 tattgccgat gaagccttaa agctcggcgt gcgggcaaaa gcgcacaaca ttcccagcct
    15361 gaaactggaa tacggcacgc tggtgccgat gcgctacatg aatgaagaca agcacttcaa
    15421 agtggtctcc atttcggctt tctgcacggt tcacgatttt gccgacagcc gcaagctggg
    15481 cgaagcgatt ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg
    15541 ttcgttatcg caccgcttta ttgacgatca gcgtgcggaa gaagggatga acagctacac
    15601 ccgcgagttc gaccgccaga tggacgagcg cgtggtgaag ttgtggcgcg aaggccagtt
    15661 caaagagttc tgcaatatgc tgccggagta cgccgactac tgctacggcg aaggcaatat
    15721 gcacgacacg gtgatgctgc tggggatgct cggctgggat aaatacgacg gcaaggtgga
    15781 gtttatcacc gagctattcc caagctctgg caccggtcag gttaacgctg ttttcccgct
    15841 tcccgcgtaa ggacgtttta tgccgcactt tatcgttaaa tgcagtgaca acatccgcga
    15901 agaagccgac ctgccggggt tgttcgccaa agtgaatccg acgctggcag ccacgggtat
    15961 ttttccgctg gcgggtattc gcagccgcgt gcattgggtc gatacctggc agatggccga
    16021 cgggcagcgt gattatgcct tcgtgcatat gacgttgaaa atcggcgcag gtcgcagcct
    16081 ggaaagccgc cagcaggcgg gtgaaatgct gtttgaactg attaaaacgc acttcgccgc
    16141 cctgatggag agccgcctgc tggcgttgtc gtttgagatt gaagagctgc atccgacgct
    16201 gaattttaaa caaaacaacg tgcacgcatt gtttaagtag cgcgcagatt gcccggtggc
    16261 gctgcgctta ccgggcctac aaaaccccaa accgtacacc gtaggccgga taaggcgcag
    16321 ccgcatccgg caatgccaca ggatatcgct atgttcgata aacacaccca caccctgatc
    16381 gcccagcgtc tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat ctcgctggat
    16441 tacccggaga tcaccatcga agacgcttac gcggtgcagc gtgaatgggt tcgcctgaaa
    16501 atcgccgaag gtcgcacgct gaaaggccac aaaatcggcc tgacctcgaa agcgatgcag
    16561 gcaagctcgc agatcagcga accggattac ggcgcgctgc tggatgacat gttcttccac
    16621 gatggcagcg atatcccgac cgatcgcttt atcgtgccgc gcattgaagt ggagctggct
    16681 ttcgtgctgg caaaaccgct gcgtgggcca aactgcacgc tgttcgacgt ctacaacgcc
    16741 acggactacg tgatcccggc gctggagctg atcgacgctc gctgccacaa catcgatccg
    16801 gaaacccagc gcccgcgcaa agtgttcgac accatttctg ataacgccgc caacgccggg
    16861 gtgatcctcg gcggtcgtcc gatcaaacca gacgaactgg atctgcgttg gatctccgcc
    16921 ctgatgtatc gcaatggcgt gattgaagaa accggcgtcg ccgctggcgt gctgaatcat
    16981 ccggcaaacg gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt acaactggaa
    17041 gccgggcaaa tcattctcgg tggttcgttc acccgcccgg ttccggcgcg taagggcgac
    17101 accttccacg tcgattacgg caacatgggt tccattagct gccgctttgt ttaaggagag
    17161 aacgatggaa aacagcttta aagcggcgct gaaagcaggc cgtccgcaga tcggattatg
    17221 gctggggctg agtagcagct acagcgcaga gttactggcc ggagcaggat tcgactggtt
    17281 gttgatcgac ggtgagcaca cgccgaataa cgtgcaaacc gtgctcaccc agctacaggc
    17341 gattgcgccc taccccagcc agccggtagt acgtccgtcg tggaacgatc cggtgcaaat
    17401 caaacaactg ctggacgtcg gcacacaaac cttgctggtg ccgatggtac aaaacgctga
    17461 cgaagcccgt gaagcggtac gcgccacccg ttatcccccc gccggtattc gcggtgtggg
    17521 cagtgcgctg gcccgcgcct cgcgctggaa tcgtattcct gattacctgc aaaaagccaa
    17581 cgatcaaatg tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga acttaccgca
    17641 gattctggac gtggaaggcg tcgacggcgt gtttatcggc ccggcggatc tgagcgccga
    17701 tatgggttat gccggtaatc cgcagcaccc ggaagtacag gccgccattg agcaggcgat
    17761 cgtgcagatc cgcgaagcgg gcaaagcgcc ggggatcctg atcgccaatg agcaactggc
    17821 aaaacgctat ctggaactgg gcgcgctgtt tgtcgccgtc ggcgttgaca ccaccctgct
    17881 cgcccgcgcc gccgaagcgc tggcagcacg gtttggtgcg caggccaccg ccgtgaagcc
    17941 cggcgtgtat taatgccgga tgcgctgcgc ttatccggcc tacactcgca ccaaaccgta
    18001 ggccggataa ggcgtttacg ccgcatccgg caaaaagccg taccaaatcg cggaccggat
    18061 aaggcgttac gccgcatccg ccataaacct tgtcgtaccc tacaaaaatc ccattagagg
    18121 aagaaaaatg agcgacacct cacctgccat accggagagt atcgatccgg cgaatcagca
    18181 taaagcgctg actgccggac aacaggcggt tattaagaag ctatttcgcc gcctgagcgt
    18241 ctttctgttc gtgctgttta tcttctcgtt ccttgatcgc atcaacatcg gctttgccgg
    18301 actcacgatg ggacgcgacc tcggtctgag cgccaccatg tttggcctcg ctaccaccct
    18361 gttctatgcc gcttatgtca tcttcggcat tcccagcaac attatgctga gtattgtcgg
    18421 tgcgcggcgc tggatcgcca ccatcatggt gctctggggc atcgcctcta ctgccaccat
    18481 gtttgccact ggccccacca gcttgtacgt actgcgtata ctggttggca ttaccgaagc
    18541 cggctttctg cctggtattc tgctgtattt aaccttctgg tttccggcct atttccgcgc
    18601 ccgtgccaac gccttgttta tggtagcaat gccggtaacg acagcgttgg gctcgatcgt
    18661 ttccggctac attttgtcgc tggatggcgt catggcatta aaaggctggc agtggctgtt
    18721 tttgctggaa ggcttcccgt cggtattact cggcgtcatg gtgtggttct ggcttgatga
    18781 ctcaccggac aaagctaagt ggctgacgaa agaagacaaa aaatgcctgc aagagatgat
    18841 ggataacgat cgtctgacgc tggttcagcc agagggagcc atcagccacc acgccatgca
    18901 acaacgcagt atgtggcggg agatcttcac tccggtggtg atgatgtata ccctggcgta
    18961 tttctgcctg accaacacac ttagtgcgat cagcatctgg acaccgcaga tcctgcaaag
    19021 ttttaatcag ggcagcagta atatcaccat cggcatgctg gccgccgtac cgcagatttg
    19081 taccattctc gggatggtct actggagccg tcactcagat cgccgccagg aacgaaggca
    19141 tcacaccgcc cttccttatt tgttcgctgc cgcaggttgg ttactggctt cggcaactga
    19201 tcacaacatg atccagatgc tggggatcat tatggcttcg accggatcat tcagcgcaat
    19261 ggcgattttc tggacaacac cggatcagtc catcagcctg cgggcacgag cgatcggtat
    19321 tgcggtgatc aacgccactg gcaacattgg ttcagcgtta agtccgttta tgatcggctg
    19381 gttgaaagat ttgaccggca gctttaacag tggattgtgg tttgttgccg cgctgctggt
    19441 gattggtgcg gggattatct gggcgattcc aatgcagtcc tcccgtccgc gagcgaccct
    19501 gtaaggaacg acgatgtgtg accgtcagat tgccaatatt gatatcagca aagagtacga
    19561 tgaaagcctg ggcacggacg atgtgcatta tcagtcgttc gcccgcatgg cggcattttt
    19621 tggccgccat atgctgccac atcgccacga acagtacttt cagatgcatt tcctcaatag
    19681 cggacagatt gagctacagc ttgacgatca tcgctactcg gtggaagcgc ccctgtttgt
    19741 cctgacgccg ccgtca
//
LOCUS       DAZOXR010000069        19407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZOXR010000069 DAZOXR010000000
VERSION     DAZOXR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.071"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHUNN_002133"
     CDS             <1..245
                     /locus_tag="ACHUNN_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0576504.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUNN_002134"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUNN_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0576505.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUNN_002135"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUNN_002135"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR0576506.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUNN_002136"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUNN_002136"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR0576507.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHUNN_002137"
     CDS             3423..4061
                     /locus_tag="ACHUNN_002137"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0576508.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHUNN_002138"
     CDS             4066..4842
                     /locus_tag="ACHUNN_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR0576509.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHUNN_002139"
     CDS             4931..6295
                     /locus_tag="ACHUNN_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR0576510.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUNN_002140"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUNN_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0576511.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUNN_002141"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUNN_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0576512.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUNN_002142"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUNN_002142"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0576513.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUNN_002143"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUNN_002143"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0576514.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUNN_002144"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUNN_002144"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0576515.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUNN_002145"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUNN_002145"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0576516.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUNN_002146"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUNN_002146"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0576517.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUNN_002147"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUNN_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0576518.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHUNN_002148"
     CDS             complement(12844..13167)
                     /locus_tag="ACHUNN_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0576519.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUNN_002149"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUNN_002149"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0576520.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUNN_002150"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUNN_002150"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0576521.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUNN_002151"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUNN_002151"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0576522.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHUNN_002152"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHUNN_002152"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0576523.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHUNN_002153"
     CDS             complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHUNN_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR0576524.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHUNN_002154"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHUNN_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="ACHUNN_002155"
     CDS             complement(18926..>19407)
                     /gene="cas6e"
                     /locus_tag="ACHUNN_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0576525.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4592 a         5230 c         4847 g         4738 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgtgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagc
//
LOCUS       DAZOXR010000070        18844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZOXR010000070 DAZOXR010000000
VERSION     DAZOXR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.072"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..103)
                     /locus_tag="ACHUNN_002156"
     CDS             complement(<1..103)
                     /locus_tag="ACHUNN_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576526.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHUNN_002157"
     CDS             complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHUNN_002157"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0576527.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHUNN_002158"
     CDS             complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHUNN_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0576528.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHUNN_002159"
     CDS             complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHUNN_002159"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0576529.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHUNN_002160"
     CDS             complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHUNN_002160"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0576530.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHUNN_002161"
     CDS             complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHUNN_002161"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0576531.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHUNN_002162"
     CDS             complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHUNN_002162"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0576532.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="ACHUNN_002163"
     CDS             complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="ACHUNN_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0576533.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9873..10451
                     /locus_tag="ACHUNN_002164"
     CDS             9873..10451
                     /locus_tag="ACHUNN_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0576534.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10495..11970
                     /gene="ampG"
                     /locus_tag="ACHUNN_002165"
     CDS             10495..11970
                     /gene="ampG"
                     /locus_tag="ACHUNN_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0576535.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12431..13378
                     /gene="cyoA"
                     /locus_tag="ACHUNN_002166"
     CDS             12431..13378
                     /gene="cyoA"
                     /locus_tag="ACHUNN_002166"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR0576536.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13400..15391
                     /gene="cyoB"
                     /locus_tag="ACHUNN_002167"
     CDS             13400..15391
                     /gene="cyoB"
                     /locus_tag="ACHUNN_002167"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR0576537.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15381..15995
                     /locus_tag="ACHUNN_002168"
     CDS             15381..15995
                     /locus_tag="ACHUNN_002168"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0576538.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15995..16324
                     /locus_tag="ACHUNN_002169"
     CDS             15995..16324
                     /locus_tag="ACHUNN_002169"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0576539.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16336..17226
                     /gene="cyoE"
                     /locus_tag="ACHUNN_002170"
     CDS             16336..17226
                     /gene="cyoE"
                     /locus_tag="ACHUNN_002170"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0576540.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17411..17722
                     /locus_tag="ACHUNN_002171"
     CDS             17411..17722
                     /locus_tag="ACHUNN_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0576541.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17706..18248
                     /locus_tag="ACHUNN_002172"
     CDS             17706..18248
                     /locus_tag="ACHUNN_002172"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0576542.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18304..>18844)
                     /locus_tag="ACHUNN_002173"
     CDS             complement(18304..>18844)
                     /locus_tag="ACHUNN_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0576543.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4955 c         4580 g         5093 t
ORIGIN      
        1 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
       61 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
      121 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
      181 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
      241 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
      301 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
      361 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
      421 gaaccacatt accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca
      481 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
      541 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc ccctttgccg gctttcaaat
      601 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
      661 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
      721 tgatgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
      781 gcgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
      841 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
      901 cagcttgctc tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct
      961 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
     1021 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
     1081 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
     1141 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
     1201 cagagataat atcagcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
     1261 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
     1321 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
     1381 cgctaaccgg ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt
     1441 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
     1501 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
     1561 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
     1621 caccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
     1681 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
     1741 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
     1801 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
     1861 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
     1921 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
     1981 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
     2041 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
     2101 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
     2161 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
     2221 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
     2281 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
     2341 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
     2401 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
     2461 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
     2521 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
     2581 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
     2641 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
     2701 cccgatccgc catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
     2761 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
     2821 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
     2881 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
     2941 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
     3001 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
     3061 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
     3121 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
     3181 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
     3241 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
     3301 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
     3361 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
     3421 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
     3481 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
     3541 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
     3601 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
     3661 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
     3721 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
     3781 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
     3841 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
     3901 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
     3961 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
     4021 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
     4081 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
     4141 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
     4201 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
     4261 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
     4321 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
     4381 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
     4441 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
     4501 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
     4561 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
     4621 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
     4681 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
     4741 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
     4801 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
     4861 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
     4921 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
     4981 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
     5041 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
     5101 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
     5161 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
     5221 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
     5281 aattaaaggg gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat
     5341 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
     5401 ccatcaatta ccgactcgtc gataaccact ttttcgacgt cttccatgga cggcagatcg
     5461 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
     5521 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
     5581 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
     5641 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
     5701 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
     5761 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
     5821 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
     5881 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
     5941 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct
     6001 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
     6061 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
     6121 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
     6181 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
     6241 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
     6301 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
     6361 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
     6421 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
     6481 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
     6541 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
     6601 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
     6661 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg
     6721 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
     6781 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
     6841 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
     6901 cacgggcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
     6961 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcagcaaga
     7021 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
     7081 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
     7141 gatagatatc tttttctggg ttttccgctt ctaggaacag catctgcgcc acaatcaggt
     7201 tcgccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
     7261 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
     7321 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
     7381 aattgaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg
     7441 tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca
     7501 ccgaaagagg cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc
     7561 tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg
     7621 gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc
     7681 ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt
     7741 gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag
     7801 gcctttcacg cgctcttcgt cagctttcag ttcgttggtg cggataactt cgcccagcag
     7861 caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag
     7921 agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc
     7981 gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc
     8041 ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac
     8101 ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc
     8161 agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc
     8221 tttacctttc agattttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc
     8281 ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc
     8341 catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc
     8401 ggtgaagtcg atggttacgc ggtcttctgc ttcaacggcg ccgtcttttt ctttccaggt
     8461 cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac
     8521 gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc
     8581 aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg
     8641 tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag
     8701 gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg
     8761 cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct
     8821 tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc
     8881 ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca
     8941 actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa
     9001 tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc
     9061 ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt
     9121 cccgggcaat tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg
     9181 gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc
     9241 aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt
     9301 cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag
     9361 tagagagttc ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac
     9421 ccgtaaaacg atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat
     9481 tgtgacgata gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt
     9541 tttcttctat ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca
     9601 tccatccctt taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt
     9661 taggtttcgt cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac
     9721 acaggcctcg catcagcgtt tcttttacca ggggtgatta tttcgccaac atgatgaatt
     9781 tacatgcctt acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc
     9841 aaacacgctt aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag
     9901 ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga
     9961 cgattacact gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg
    10021 ccgatcagcg taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc
    10081 tgaccgcctc ccgagatctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg
    10141 cgcgtggtta catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac
    10201 tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat cgcgaccaaa gcggatatcg
    10261 ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca
    10321 acgttgaagg tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc
    10381 tgaccgatac catcgctgat atgtctcagg acaccagcat ccacgaattc atcaagcaga
    10441 acgcgcgtta atttctgccc tctggcccgg tgcaaaccgg gcctgtagac gcccatgtcc
    10501 agtcaatatt tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt
    10561 gcttccgggc taccgctcgc cctgacatcc ggtaccttac aggcctggat gacggtcgag
    10621 aatattgatc tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa
    10681 ttcctctggt caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt
    10741 tggctgctcg ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa
    10801 cctggcaccc aactccgctg gatggcggca ctggcggtgg tgatcgcttt ttgctcggcc
    10861 tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt
    10921 gcgggcgcgg caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggctta
    10981 gccctgtggc tggcagataa atggctgggc tggcagggca tgtactggtt gatggcggcg
    11041 ctgttgatcc cttgtattat cgcgacgttg cttgcaccag aaccgaccga caccattcct
    11101 gtgccaaaaa cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat
    11161 aatgcctggc ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg
    11221 agcctgacaa ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg
    11281 gttaacaaaa cgcttggctt actggcgacc attgtgggcg cattgtacgg tgggattttg
    11341 atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct
    11401 aacgctggtt actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc
    11461 gtctttttcg aaaacctctg tggcgggatg ggcacatcag cctttgtcgc actgttaatg
    11521 acgctgtgta ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct
    11581 gtaggacgag tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg
    11641 acattctatc tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc
    11701 cgccagacgc ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg
    11761 acaggttatg cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg
    11821 ttactgctgt tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg
    11881 ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat
    11941 ctggctctac gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca
    12001 ctcgccgtaa ttattacggc taaataaaca tcagtatcgc ttaataatat ttgttctttt
    12061 gtgcggctta gcgtttggaa attattgacg caatttatga attctatttt ttcttacacg
    12121 attcagctaa tgagtcttta ttttcccccc acccaattgt caccataatg ataattattt
    12181 gttaaacaat tgttttattc aagattggtt ataccaattg cccgtccaaa ctccttctgc
    12241 actccccttt cgttataacg cccttttgca acagcttctt aaaatcaact tgatatgttt
    12301 tgcaacatat gtaacctgac agccaaatcc aagtaacagg aatttaatca tgtttacagt
    12361 aatgtaacct ccccgtaaaa tgcccacaca ctttaaacgc caccagatcc cgtggaattg
    12421 aggtcgttaa atgagactca ggaaatacaa taaaagtttg ggatggttgt cattatttgc
    12481 aggcactgta ttgctcagtg gctgtaattc tgcgctgtta gatcccaaag gacagattgg
    12541 tctggagcaa cgttcactga tactgacggc atttggcctg atgttgattg tcgttattcc
    12601 cgcaatcttg atggctgttg gtttcgcctg gaagtatcgt gcgagcaata aagatgctaa
    12661 gtacagcccg aactggtcac actccaataa agtggaagct gtggtctgga cggtacctat
    12721 cttaatcatc atcttccttg cggtactgac ctggaaaacc actcacgctc ttgagcctag
    12781 caagccgctg gcacacgacg agaagcccat taccatcgaa gtggtttcca tggactggaa
    12841 atggttcttc atctacccgg aacagggcat tgcaaccgtg aatgaaatcg ctttcccggc
    12901 gaacactccg gtgtacttca aagtgacctc caactccgtg atgaactcct tcttcattcc
    12961 gcgtctgggt agccagattt atgccatggc cggtatgcag actcgcctgc atctgatcgc
    13021 caacgaaccc ggtacttatg acggtatctc cgccagctac agcgggccgg gcttctcagg
    13081 catgaagttc aaagctattg caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa
    13141 agcgaaacag tcgccgaaca ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc
    13201 tagcgaatac aaccaggtgg aatatttctc caacgtgaaa ccagacttgt ttgccgatgt
    13261 gattaacaag tttatggctc acggtaagag catggacatg acccagccag aaggtgagca
    13321 cagtgcacac gaaggtatgg aaggcatgga catgagccac gcggaatccg cccattaaag
    13381 gggttgagga agaataaaga tgttcggaaa attatcactt gatgcagtcc cgttccatga
    13441 acctatcgtc atggttacga tcgctggcat tattttggga ggtctggcgc tcgttggcct
    13501 gatcacttac ttcggtaagt ggacctacct gtggaaagag tggctgacct ccgtcgacca
    13561 taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg atgttgctgc gtggttttgc
    13621 tgacgccatt atgatgcgta gccagcaggc tcttgcctcg gcgggcgaag cgggcttcct
    13681 gccacctcac cactacgatc agatcttcac cgcgcacggc gtgattatga tcttcttcgt
    13741 ggcgatgcct ttcgttatcg gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg
    13801 tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg tttactgttg ttggtgtgat
    13861 tctggttaac gtttctctcg gcgtgggcga atttgcgcag accggctggc tggcctatcc
    13921 accgctatcg ggaatagagt acagtccggg agtcggtgtc gattactgga tatggagtct
    13981 ccagctatcc ggtataggta cgacgcttac cggtatcaac ttcttcgtta ccattctgaa
    14041 gatgcgcgca ccgggcatga ccatgttcaa gatgccagta tttacctggg catcactgtg
    14101 cgcgaacgta ctgattattg cttccttccc aattctgacg gttaccgtcg cgttgttgac
    14161 cctggatcgc tatctgggca cccatttctt taccaacgat atgggtggca acatgatgat
    14221 gtacatcaac ctgatttggg cctggggcca cccggaagtt tacatcctga tcctgcctgt
    14281 tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac
    14341 ctcgctggta tgggcaaccg tctgtatcac cgtgctgtcg ttcatcgttt ggctgcacca
    14401 cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc tttggtatca ccacaatgat
    14461 tatcgccatc ccgaccgggg tgaagatctt caactggctg ttcaccatgt atcagggccg
    14521 catcgtgttc cattctgcga tgctgtggac catcggtttt atcgtcactt tctcggtggg
    14581 cgggatgact ggcgtgctgc tggcagtacc gggcgcggac ttcgttctgc ataacagcct
    14641 gttcctgatt gcgcacttcc ataacgttat catcggcggc gtggtcttcg gctgcttcgc
    14701 agggatgacc tactggtggc ctaaagcgtt cggtttcaaa ctgaatgaaa cctggggtaa
    14761 acgcgcgttc tggttctgga tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct
    14821 gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt gacccgcagt tccacaccat
    14881 gctgatgatt gcagctagcg gtgcagtact gattgcgctg ggtattctct gcctcgttat
    14941 tcagatgtac gtttctattc gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg
    15001 gggtggccgt acgctggagt gggcaacctc ttccccgcct ccgttctata actttgccgt
    15061 tgtgccgcac gttcacgagc gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta
    15121 taaaaagcct gaccactatg aagaaattca tatgccgaaa aacagcggtg caggtatcgt
    15181 cattgcagct ttctccacca tcttcggttt cgccatgatc tggcatatct ggtggctggc
    15241 gattgttggc ttcgcaggca tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt
    15301 ggattactac gtgccggtgg cagaaatcga aaaactggaa aaccagcatt tcgatgagat
    15361 tactaaggca gggctgaaaa atggcaactg atactttgac gcacgcgact gcccacgcgc
    15421 acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt cggattttgg atctacctga
    15481 tgagcgactg cattctgttc tcaatcttgt ttgctaccta tgccgttctg gtgaacggca
    15541 ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc gttcgttctg gttgaaactt
    15601 tcttgctgtt gttcagctcc atcacctacg gcatggcggc tatcgccatg tacaaaaaca
    15661 acaaaagcca ggttatctcc tggctggcgt tgacctggtt gtttggtgcc ggatttatcg
    15721 ggatggaaat ctacgaattc catcacctga ttgttaacgg catgggtccg gatcgcagcg
    15781 gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg tctgcacgtc acttccggtc
    15841 ttatctggat ggcggtgctg atggtacaaa tcgctcgtcg cggcctgacc agcactaacc
    15901 gtacccgcat catgtgcctg agcctgttct ggcacttcct ggatgtggtt tggatctgtg
    15961 tgttcactgt tgtttatctg atgggggcga tgtaatgagt cattctaccg atcacagcgg
    16021 cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt atcctgtcga tcattctgac
    16081 ggtgattccg ttctggatgg tgatgacagg agctgcctct ccggccgtaa ttctgggaac
    16141 aatcctggca atggcagtgg tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa
    16201 taccaaatca gatgaaggct ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc
    16261 tatcctggtt gtaggctcca tctggattat gtggaacctc aactacaaca tgatgatgca
    16321 ctaagagcgg cggttatgat gtttaagcaa tacctgcaag taacgaaacc aggcatcatc
    16381 tttggcaacc tgatctcggt gattggggga ttcctgctgg cctcaaaggg cagcattgat
    16441 tatcccctgt ttatctacac gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg
    16501 tttaacaact acatcgacag ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg
    16561 ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt acgccacctt gctaggcttt
    16621 gctggcttta tgctgctgtg gtttggcgcg aatccgctgg cctgctggct gggggtgatg
    16681 ggctttgtgg tttatgtcgg cgtttatagc ctgtacatga aacgccactc ggtctacggc
    16741 acattgattg gttcgctctc cggcgctgcg ccgccggtga ttggctactg tgcagtaacc
    16801 ggcgagttcg atagcggcgc agcgatcctg ctggctatct tcagcctgtg gcagatgcct
    16861 cactcctatg ccatcgccat tttccgcttt aaggattatc aggcggcaaa cattccggta
    16921 ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc
    16981 tttgccgttg ccacgctgat gctctctctt ggcggttacg ctgggtataa atatctggtg
    17041 gtcgccgcgg cggtgagcgt ctggtggtta ggtatggccc tgcgcggtta taaagttgct
    17101 gatgacagga tctgggcgcg caagctgttc ggcttctcta tcatcgccat cacagccctc
    17161 tcggtgatga tgtccgttga ttttatggtg ccggactcgc atacgctgct ggctgctgtt
    17221 tggtaacaca acctctctat taaaaaggtg ctacggcacc ttttttattg tctgagatca
    17281 gagatacggc taataaccgt atgtcttgac agagtgagca gatgaacata aattaatcat
    17341 acggttgata gccgtattat gtatgagatc gactccctac gatttcattc agttaatcaa
    17401 agaggtcata atgcctggaa aaacagccac tctacccgat gtcgataaaa cgctgaaaaa
    17461 tgcgcgggtc gaactcaaaa ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca
    17521 agccgttggg gtagatttaa gcgcttttat tttgagcgct gctatggaac gcgccgaaag
    17581 tgtgcttgat aaccaacgtc gccgtgagct ttcgaatcaa agctgggaac tgatgaacca
    17641 actcatcgct gaacctgctc aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa
    17701 cagcgatggt cgacaagcat gaagagatta ctctgcccat agtcctctcc tgtaattatc
    17761 agtctgatat tacttatcct gggcaaaaac agtttgattg cggtaaccct gttatcgata
    17821 aatttgtacg cgcatcgcta aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac
    17881 ttattgacag acaaagtggt gaactgatcg gcatctgtac ttttacggca tattcgctgg
    17941 aaaaacaacg cgtttctggc gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca
    18001 gattagtcat gttgggggta gcacggaagt atcaaaagcg gggctttggt caggacctac
    18061 tatgtgattt ttttgaacat gtaaaaataa ttcaccaggc attaccaatt aaaggggttt
    18121 atcttgatgc tgaccctgcc gccattaatt tttatgctcg tctcggcttt gttcagcttt
    18181 cagcgacacc aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg
    18241 cggcttagat tattatcgcc acccgccagg gcaagttttg ccttagccgg gggctgagca
    18301 gcgttaccac ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat
    18361 ggttgccgtt gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc
    18421 cggtttataa agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa
    18481 gctaatattc agccggttta aaccgaaagg cgcgaagcca accgtttggt taatctcctt
    18541 accggatggg cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt
    18601 ggtggtggcg aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatctcgcat
    18661 cccagaacgg gtaaccgtaa tcacgggtaa cgtatcgtca accgtttttc tgtcaatcat
    18721 gtcttttcga caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc
    18781 aagatattcc ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacataat
    18841 gccc
//
LOCUS       DAZOXR010000071        18836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZOXR010000071 DAZOXR010000000
VERSION     DAZOXR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.073"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(81..2072)
                     /gene="tkt"
                     /locus_tag="ACHUNN_002174"
     CDS             complement(81..2072)
                     /gene="tkt"
                     /locus_tag="ACHUNN_002174"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0576544.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2350..3108
                     /gene="loiP"
                     /locus_tag="ACHUNN_002175"
                     /gene_synonym="yggG"
     CDS             2350..3108
                     /gene="loiP"
                     /locus_tag="ACHUNN_002175"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0576545.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3251..4171)
                     /gene="speB"
                     /locus_tag="ACHUNN_002176"
     CDS             complement(3251..4171)
                     /gene="speB"
                     /locus_tag="ACHUNN_002176"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0576546.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4309..6285)
                     /gene="speA"
                     /locus_tag="ACHUNN_002177"
     CDS             complement(4309..6285)
                     /gene="speA"
                     /locus_tag="ACHUNN_002177"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0576547.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6294..6425)
                     /gene="yqgB"
                     /locus_tag="ACHUNN_002178"
     CDS             complement(6294..6425)
                     /gene="yqgB"
                     /locus_tag="ACHUNN_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0576548.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6519..6662)
                     /gene="yqgG"
                     /locus_tag="ACHUNN_002179"
     CDS             complement(6519..6662)
                     /gene="yqgG"
                     /locus_tag="ACHUNN_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0576549.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6773..7024)
                     /gene="yqgD"
                     /locus_tag="ACHUNN_002180"
     CDS             complement(6773..7024)
                     /gene="yqgD"
                     /locus_tag="ACHUNN_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0576550.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7080..8234
                     /gene="metK"
                     /locus_tag="ACHUNN_002181"
     CDS             7080..8234
                     /gene="metK"
                     /locus_tag="ACHUNN_002181"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0576551.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            8670..10064
                     /gene="galP"
                     /locus_tag="ACHUNN_002182"
     CDS             8670..10064
                     /gene="galP"
                     /locus_tag="ACHUNN_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0576552.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            10141..10638
                     /locus_tag="ACHUNN_002183"
     CDS             10141..10638
                     /locus_tag="ACHUNN_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0576553.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            10733..11440
                     /gene="endA"
                     /locus_tag="ACHUNN_002184"
     CDS             10733..11440
                     /gene="endA"
                     /locus_tag="ACHUNN_002184"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0576554.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            11520..12251
                     /gene="rsmE"
                     /locus_tag="ACHUNN_002185"
     CDS             11520..12251
                     /gene="rsmE"
                     /locus_tag="ACHUNN_002185"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0576555.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            12264..13214
                     /gene="gshB"
                     /locus_tag="ACHUNN_002186"
     CDS             12264..13214
                     /gene="gshB"
                     /locus_tag="ACHUNN_002186"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0576556.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            13323..13886
                     /locus_tag="ACHUNN_002187"
     CDS             13323..13886
                     /locus_tag="ACHUNN_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0576557.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            13886..14302
                     /gene="ruvX"
                     /locus_tag="ACHUNN_002188"
     CDS             13886..14302
                     /gene="ruvX"
                     /locus_tag="ACHUNN_002188"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0576558.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(14477..15457)
                     /locus_tag="ACHUNN_002189"
     CDS             complement(14477..15457)
                     /locus_tag="ACHUNN_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0576559.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            15475..16179
                     /gene="yggS"
                     /locus_tag="ACHUNN_002190"
     CDS             15475..16179
                     /gene="yggS"
                     /locus_tag="ACHUNN_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0576560.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16197..16763
                     /gene="yggT"
                     /locus_tag="ACHUNN_002191"
     CDS             16197..16763
                     /gene="yggT"
                     /locus_tag="ACHUNN_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0576561.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            16760..17050
                     /gene="yggU"
                     /locus_tag="ACHUNN_002192"
     CDS             16760..17050
                     /gene="yggU"
                     /locus_tag="ACHUNN_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0576562.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            17058..17651
                     /locus_tag="ACHUNN_002193"
     CDS             17058..17651
                     /locus_tag="ACHUNN_002193"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0576563.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            17644..18780
                     /gene="hemW"
                     /locus_tag="ACHUNN_002194"
     CDS             17644..18780
                     /gene="hemW"
                     /locus_tag="ACHUNN_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0576564.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         4484 a         5017 c         4922 g         4413 t
ORIGIN      
        1 tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg ccgaagcgac
       61 ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac gttgtcgacg
      121 gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa ggtggtcata
      181 ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat acccgcttct
      241 accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc cgcatcctgc
      301 ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc ttcggcagtc
      361 agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat gaagatcagt
      421 tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt tgccagttgc
      481 tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc ggtcggaccg
      541 tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc acacggacgc
      601 catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg ctggtgagtc
      661 gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg acgctgtttc
      721 atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag gaaggtggag
      781 gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt cataccgaac
      841 tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc tttagaacca
      901 gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa ttccggcaac
      961 agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat tttcgccgga
     1021 ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga cggcatttcg
     1081 cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc gtaagccgca
     1141 aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc ccactgcgca
     1201 tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc gcgggtcaga
     1261 gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc tttgttcggg
     1321 gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt cactgcgcgc
     1381 gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc gcgaataacg
     1441 tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc ttcaacgtga
     1501 ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag cttcagcgta
     1561 cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc gcccatgaag
     1621 gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc cgccagcgtt
     1681 ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg accggtggtg
     1741 gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga gtgcagctga
     1801 cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag caggctgtag
     1861 atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc ccaggacgga
     1921 ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc agccataccc
     1981 ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct cagcgcacga
     2041 atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat gatgaagggc
     2101 acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt tgccaacaat
     2161 ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag aaatctctgg
     2221 atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc ttatactgag
     2281 actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac cttgtgcgga
     2341 agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac ggtactgact
     2401 ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc ttttcaggct
     2461 tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca ggagatggac
     2521 agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac aactattgcc
     2581 aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta tatggcgaag
     2641 gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg gctgatggat
     2701 atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca cgtggcgtta
     2761 ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg agtagctgcg
     2821 gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga tctgggcgag
     2881 aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga ttattcttac
     2941 gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt tgaaaaactg
     3001 gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc atccgccgaa
     3061 cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag cctggtggtg
     3121 tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca catttgttac
     3181 cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg ggtggcatcg
     3241 catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc agcgccagcg
     3301 tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact tccactacgt
     3361 ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg cgatcggagg
     3421 tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc aggcagtcga
     3481 tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg gcgataacgt
     3541 catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg ttgtctttat
     3601 caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga ccttctttcg
     3661 gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag gtatcggtgt
     3721 gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca cgcagcagcg
     3781 gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca gccagcagct
     3841 tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag gcatatacca
     3901 gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac gggaagcggt
     3961 tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg tggcgaccac
     4021 ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag tctgcatcgc
     4081 tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg gaaaccagtg
     4141 agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa agtcgatata
     4201 aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc acaataaatt
     4261 gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt actcatcttc
     4321 aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt gttgcagttc
     4381 agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg ttttcggatc
     4441 gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca gttctacttc
     4501 tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga acaggttgtg
     4561 catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca tcggcggatt
     4621 ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat caatatagtg
     4681 gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac gttccggcac
     4741 ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc atgcgtccgg
     4801 cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt cctgcagttc
     4861 gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca cttcatggca
     4921 catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg tgccggaaga
     4981 gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga
     5041 acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt gcagcgcgcg
     5101 cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca cgccgatgat
     5161 attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt
     5221 cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga tgttgttggc
     5281 gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc cttcatagtc
     5341 cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct tgtgcagttc
     5401 cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat tcgccatctg
     5461 cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt cacgcagggt
     5521 ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt ccccgccgga
     5581 ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca gacgaggaac
     5641 gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct tctcaatgac
     5701 cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat attcgcggtc
     5761 tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca gtactgccat
     5821 caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa tcagggactc
     5881 aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat cgccgttata
     5941 gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag
     6001 gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac gagttttcac
     6061 taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca cgctgatgtg
     6121 gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt aagtacgcag
     6181 catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta gtacaccgtg
     6241 ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga acctcaaatt
     6301 attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga taacaaccca
     6361 taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac cggtttcttt
     6421 ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg aaaccacgag
     6481 aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta accagctctt
     6541 gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc agagatgcag
     6601 agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag acaacggttc
     6661 attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc aaaactctga
     6721 tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg
     6781 cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata cgcagaatgc
     6841 cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac gattcaaaaa
     6901 aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta atccatccat
     6961 accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta ggatccgcta
     7021 ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg atattaaata
     7081 tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac aaaattgctg
     7141 accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg
     7201 cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc accaccagcg
     7261 cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc tatgtgcatt
     7321 ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc aaacagtctc
     7381 ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg ggtgaccagg
     7441 gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca cctatcacct
     7501 atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc
     7561 tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa atcgttggta
     7621 tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa tcgctgcaag
     7681 aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg acttctgcca
     7741 ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg ggtgactgcg
     7801 gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt cacggtggcg
     7861 gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt
     7921 atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt caggtttcct
     7981 acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt actgagaaag
     8041 tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc ccatacggtc
     8101 tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca tacggtcact
     8161 ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg
     8221 ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc tggccttttt
     8281 cttttacctc accgcggtgg ataggcattc acgccgcatc cggcaaaaag ctgcccgcac
     8341 aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac tgaaaccgat
     8401 tacaccaata acaccagcca aatatttgta atattttcat attattattc agttttcaca
     8461 gttgttacat ttcttttcag taaagtctta attgcagata acagcgttta atctatgatg
     8521 atataactca attattttca tgcacttaaa ccataactaa gataaatgtt agtgtaagcg
     8581 attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat cttaattcac
     8641 aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag gggcggtcaa
     8701 acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta ctctttggcc
     8761 tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc cagattactt
     8821 cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc ggtgcggtgg
     8881 gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc ggcgcaattt
     8941 tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta ctgattcttt
     9001 cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg ctgtacctct
     9061 ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag ttgatgatca
     9121 ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc ggtgcatggc
     9181 gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt gtcttcttcc
     9241 tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc gaacgcgtgc
     9301 tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa atccgtgaaa
     9361 gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac ttccgccgcg
     9421 cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg aacgtcatca
     9481 tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccaca gagcaaatgt
     9541 gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca atcggccttg
     9601 ttgaccgctg gggacgaaaa ccaacgctaa cgctgggctt cctggtgatg gctgctggca
     9661 tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg cagtatttcg
     9721 ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt ccgctgattt
     9781 gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc acctgctcca
     9841 ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc atgctcaaca
     9901 cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg tttatcctgc
     9961 tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt gaacgtaacc
    10021 tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct caggcttcct
    10081 cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc ctctgccgct
    10141 atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgcgg cctgcgggaa
    10201 aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa actctcttac
    10261 acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat ccgcctcaat
    10321 cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc gcacgaactg
    10381 gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa agagtggaag
    10441 tggatgatgg aaagcgtgct gggtgttccc gcccgtcgta cgcatcagtt cgaactgcaa
    10501 tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca gcttaccgta
    10561 cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca ctgcggtgaa
    10621 cagctggttg cgaaataacc atctgaacta tcaggaactt tcctgatctg gctgattgca
    10681 taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag tgatgtaccg
    10741 ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg cgttggccga
    10801 aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg ctgacgcgcc
    10861 cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg ttgttgatct
    10921 gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg tagagtggga
    10981 acacgtcgtt cccgcctggc agttcggtca ccagcgccag tgctggcagg acggtggacg
    11041 taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgt ataacctgca
    11101 gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc agtggaatgg
    11161 cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa aagctgccga
    11221 accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc gcgaccaata
    11281 caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca agatgtatcc
    11341 ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag gcaatcataa
    11401 cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag cgggattctt
    11461 tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga tttttaacta
    11521 tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac atcgcgcttt
    11581 gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg caggcgttgc
    11641 aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc agcaaaaaaa
    11701 gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg ctgcatattc
    11761 acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag aaatcgatcg
    11821 aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt aaactggata
    11881 gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct gcctgtgagc
    11941 agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa gcctggtgtg
    12001 cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac agcatcaata
    12061 cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc ggtttatcgg
    12121 cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg ggacctcgcg
    12181 ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta cgatttggcg
    12241 atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac cccatcgcaa
    12301 acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag cgtcgtggtt
    12361 acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa gcccgcgccc
    12421 atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc gtcggtgaac
    12481 aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg ccgtttgata
    12541 ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg acgctgatcg
    12601 ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc tggttctctg
    12661 acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg ttctgggaga
    12721 aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg attttccgcg
    12781 tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag aatggcactc
    12841 gctactgcat ggcacaaaat tacctgccag ccattaaaga tggcgacaaa cgcgtgctgg
    12901 tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg ggcgaaaccc
    12961 gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa agtgactgga
    13021 aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt gttggtctgg
    13081 atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt attcgtgaga
    13141 ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc gaagcacgtt
    13201 tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga gtgacagcgc
    13261 ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca gaacctctga
    13321 caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat ccgattttcc
    13381 gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg atcatcgtca
    13441 acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag atcacgccgg
    13501 agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt ccgctggctg
    13561 aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc attcgcattt
    13621 cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc accgataaac
    13681 aaccgtctga cgtattggtg gctctaggtt atgcctcctg ggaaaaaggt caactggaac
    13741 aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt ctgttcaaaa
    13801 cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat attctcacca
    13861 tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg acttcggcac
    13921 caaaagcatt ggcgtagcgg tcggccaacg cattaccggc actgctcgcc ctttgcctgc
    13981 aattaaagca caggacggta cgccggactg gaacctcatc gaacgtttac tgaaagagtg
    14041 gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg agcaaccatt
    14101 gactgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg ttgaagtaaa
    14161 gctccatgac gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg aacagggcgg
    14221 ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta ttctcgaaag
    14281 ctatttcgag cagggatatt aaggcgattt aaaagcctgg cggatagtcg ttaccgccat
    14341 cttgccggat gcggcgtgaa cgcctatccg gcctacatgt ggcagcgatt gtaggtctga
    14401 taagacgcgc aaacgtctca tcagacgttt attgcctttg taggcccgat aagcttgcac
    14461 atcgggcatg gcaacgtcac aaacgccctt cccccactcg ctgctgaaaa ctctgctgaa
    14521 acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg tgggttttcc
    14581 cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac agcgccacgc
    14641 gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa ctacctgcca
    14701 gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt cgctcaactg
    14761 cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt tctgccgccg
    14821 tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc acatcaggat
    14881 cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa ccaatttccc
    14941 gcttctggat caaacatcgc tggctggcat agatatattc aacaggatct tccagcgtca
    15001 gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc gtggtagatt
    15061 tgctgctccc cgtcgccccc gtcaccagaa tcaggccatt ctcactcttg agtaattccg
    15121 gcaataccgt tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt aacagccgta
    15181 acgccagcga aatgccatgc cgttgcgcaa atgcgctgcc acgcaatcgc tggttttccg
    15241 ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc tgatcgtcat
    15301 ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca gcttccatta
    15361 gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc gagacgttat
    15421 gctttacact aagggccaca atttcttcca tattcatact aagatcctcg gaaaatgaac
    15481 gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc aacgcgttgc
    15541 ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc
    15601 atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt tcaggaaggg
    15661 gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg gcattttatt
    15721 ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg gtgtcatacc
    15781 atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga acttccccct
    15841 cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg gattcaactg
    15901 gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg tctgcgcggg
    15961 ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa
    16021 atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac gctctctctg
    16081 ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt tcgtatcggc
    16141 actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg aacgccatga
    16201 atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg ctgttattac
    16261 gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag tttgtagtga
    16321 aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg ggccccattg
    16381 acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc gtgctgttta
    16441 aagtggtgac cttcctgcca atcatctgga ttgctggttt actgattctg ctgaaaacca
    16501 tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg gtaagccagg
    16561 gtcgtagtcc gattgaatac gtgctgattc agctggccga tccgctgctg cgcccgattc
    16621 gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc gttctgctgc
    16681 tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac atgctgctgc
    16741 cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc tggttttacg
    16801 gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg gcgacgaagt
    16861 taaagtcgcc attaccgcgc cgccggttga cggccagggc aacagtcatc tggtgaagtt
    16921 tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg
    16981 ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa tcgtggcgtt
    17041 aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc
    17101 gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa acagacctcg
    17161 gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt ctgaaagcgc
    17221 gctatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt ctggcggtag
    17281 atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg
    17341 atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc
    17401 aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg actccgctgg
    17461 tatgccacgg cagctggccg ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg
    17521 gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg
    17581 aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg gacgctttac
    17641 gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa
    17701 tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca cgacgattat
    17761 gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg ccgtgaagta
    17821 aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc gatgcaaacg
    17881 ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat tactatggaa
    17941 gcgaaccctg gaacggtaga agccgatcgc tttgtcgatt atcagcgtgc tggtgtgaac
    18001 cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact tgggcgtatt
    18061 catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg gctgcgtagc
    18121 tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc gcttggcgat
    18181 ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact gaccatcgaa
    18241 cccaatacgc tgtttggttc gcgaccaccg gtgctgccgg acgatgacgc gctgtgggat
    18301 attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata tgaaacttcc
    18361 gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg atttggtgac
    18421 tacatcggta ttggctgcgg cgcgcacggc aaagtcacct tcccggatgg gcgcattctg
    18481 cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct ggaaagccag
    18541 cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt ccgtctgctg
    18601 gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga tgtgattcgc
    18661 ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga ttactggcag
    18721 ataaccgagc acggtaaatt attcttaaac tcacttcttg aattattcct cgctgagtaa
    18781 tcattaatgc gcctgcgggc gcattaatca cttctttccc ccccgttaat taactt
//
LOCUS       DAZOXR010000072        18523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZOXR010000072 DAZOXR010000000
VERSION     DAZOXR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.074"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHUNN_002195"
     CDS             <1..1710
                     /locus_tag="ACHUNN_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR0576565.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHUNN_002196"
     CDS             1711..3360
                     /locus_tag="ACHUNN_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0576566.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHUNN_002197"
     CDS             3365..4141
                     /locus_tag="ACHUNN_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0576567.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUNN_002198"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUNN_002198"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR0576568.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHUNN_002199"
     CDS             complement(5430..6752)
                     /locus_tag="ACHUNN_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR0576569.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUNN_002200"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUNN_002200"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR0576570.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUNN_002201"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUNN_002201"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR0576571.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUNN_002202"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUNN_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR0576572.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUNN_002203"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUNN_002203"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR0576573.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUNN_002204"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUNN_002204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0576574.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUNN_002205"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUNN_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0576575.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUNN_002206"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUNN_002206"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0576576.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAZOXR010000073        18214 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZOXR010000073 DAZOXR010000000
VERSION     DAZOXR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.075"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHUNN_002207"
     CDS             15..1091
                     /locus_tag="ACHUNN_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0576577.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHUNN_002208"
     CDS             complement(1484..1825)
                     /locus_tag="ACHUNN_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0576578.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUNN_002209"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUNN_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR0576579.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUNN_002210"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUNN_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0576580.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHUNN_002211"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHUNN_002211"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0576581.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUNN_002212"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUNN_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR0576582.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUNN_002213"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUNN_002213"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0576583.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUNN_002214"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUNN_002214"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0576584.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHUNN_002215"
     CDS             complement(7167..7826)
                     /locus_tag="ACHUNN_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0576585.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUNN_002216"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUNN_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0576586.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUNN_002217"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUNN_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR0576587.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHUNN_002218"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHUNN_002218"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0576588.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUNN_002219"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUNN_002219"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0576589.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUNN_002220"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUNN_002220"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0576590.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUNN_002221"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUNN_002221"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0576591.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUNN_002222"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUNN_002222"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0576592.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUNN_002223"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUNN_002223"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0576593.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHUNN_002224"
     CDS             complement(17270..18112)
                     /locus_tag="ACHUNN_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR0576594.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4671 a         4575 c         4392 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAZOXR010000074        18042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZOXR010000074 DAZOXR010000000
VERSION     DAZOXR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.076"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            262..567
                     /locus_tag="ACHUNN_002225"
     CDS             262..567
                     /locus_tag="ACHUNN_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576595.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            674..1318
                     /gene="gfcB"
                     /locus_tag="ACHUNN_002226"
     CDS             674..1318
                     /gene="gfcB"
                     /locus_tag="ACHUNN_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0576596.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1315..2061
                     /locus_tag="ACHUNN_002227"
     CDS             1315..2061
                     /locus_tag="ACHUNN_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0576597.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2061..4157
                     /locus_tag="ACHUNN_002228"
     CDS             2061..4157
                     /locus_tag="ACHUNN_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0576598.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4203..5342
                     /locus_tag="ACHUNN_002229"
     CDS             4203..5342
                     /locus_tag="ACHUNN_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0576599.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5330..5776
                     /gene="etp"
                     /locus_tag="ACHUNN_002230"
                     /gene_synonym="yccY"
     CDS             5330..5776
                     /gene="etp"
                     /locus_tag="ACHUNN_002230"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0576600.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5796..7976
                     /gene="etk"
                     /locus_tag="ACHUNN_002231"
     CDS             5796..7976
                     /gene="etk"
                     /locus_tag="ACHUNN_002231"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR0576601.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8091..9389)
                     /gene="appA"
                     /locus_tag="ACHUNN_002232"
     CDS             complement(8091..9389)
                     /gene="appA"
                     /locus_tag="ACHUNN_002232"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0576602.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9469..9561)
                     /gene="cbdX"
                     /locus_tag="ACHUNN_002233"
     CDS             complement(9469..9561)
                     /gene="cbdX"
                     /locus_tag="ACHUNN_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0576603.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9574..10710)
                     /gene="appB"
                     /locus_tag="ACHUNN_002234"
     CDS             complement(9574..10710)
                     /gene="appB"
                     /locus_tag="ACHUNN_002234"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0576604.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10722..12266)
                     /gene="appC"
                     /locus_tag="ACHUNN_002235"
     CDS             complement(10722..12266)
                     /gene="appC"
                     /locus_tag="ACHUNN_002235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0576605.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12400..13257)
                     /locus_tag="ACHUNN_002236"
     CDS             complement(12400..13257)
                     /locus_tag="ACHUNN_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0576606.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13314..13652)
                     /gene="hyaE"
                     /locus_tag="ACHUNN_002237"
     CDS             complement(13314..13652)
                     /gene="hyaE"
                     /locus_tag="ACHUNN_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0576607.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(13649..14236)
                     /gene="hyaD"
                     /locus_tag="ACHUNN_002238"
     CDS             complement(13649..14236)
                     /gene="hyaD"
                     /locus_tag="ACHUNN_002238"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0576608.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14233..14939)
                     /gene="hyaC"
                     /locus_tag="ACHUNN_002239"
                     /pseudo
     CDS             complement(14233..14939)
                     /gene="hyaC"
                     /locus_tag="ACHUNN_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(14958..16751)
                     /gene="hyaB"
                     /locus_tag="ACHUNN_002240"
     CDS             complement(14958..16751)
                     /gene="hyaB"
                     /locus_tag="ACHUNN_002240"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0576609.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16748..17866)
                     /gene="hyaA"
                     /locus_tag="ACHUNN_002241"
     CDS             complement(16748..17866)
                     /gene="hyaA"
                     /locus_tag="ACHUNN_002241"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0576610.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4402 a         4812 c         4695 g         4133 t
ORIGIN      
        1 caataattaa tagctttcgt aacaatttcc ttatctttaa tttgtatttt gctaagattt
       61 atcttatgct cacaagtcgt atttccagag gggatcgtta ataatcttat atttccctct
      121 gcgcaccttt cctacccgca gtatcagtat atagtgcaag cggtatgagg aaagggagat
      181 ttcacaagga tgttatactc aaagtagaca atcctgatct caagcgtacg tattgtccat
      241 gcgatgaata aaggaaaagt tatgaaacac aagctttctg caatccttat ggccttcatg
      301 ttgacgacgc cagctgcgtt tgctgcctct gaagcaacaa acgggaccga ggcaacgacg
      361 ggtactactg gcaccacaac gaccactacc ggcgcaacca cgactgctac taccactggt
      421 ggcgtggctg ctggcgctgt cggcacggcg acagttgtcg gtgtggcaac ggctgttggt
      481 gtggcgacgt tggcagttgt tgcagcaaat gacagtggcg atggcggttc tcataacacc
      541 tcaaccacca cgtccactac acgctaatac tgctttaacg cgtcgaaatt aaagttaaat
      601 gatacccggt tgcgatattc gtaccgggct ttagagattt gcattttttt aattaacctt
      661 tcaaggatta aaggtgcgcc ctcttatttt atcgattttc gcactatttc ttgcgggatg
      721 tacgcacagc cagcaaagta tggtcgatac atttcgcgcc agccttttcg ataatcagga
      781 tatcaccgta gcggatcagc agatccaggc gttgccttat tccacgatgt atttacgcct
      841 taatgaaggg caacgaatct ttgtggtact gggatatata gaacaagaac aaagcaaatg
      901 gttatcccag gataacgcca tgctggttac ccacaatgga cgtcttttaa aaaccgtcaa
      961 acttaataat aatctgctgg aagtgacaaa ttccgggcag gaccctctgc gtaacgcgct
     1021 ggcaataaaa gatggcagcc gctggacgcg cgatattctc tggagtgaag acaaccattt
     1081 tcgctctgcg accctgagtt ctactttttc ctttgctgga ttagagacgc tgaatattgc
     1141 gggtcgcaat gtgctgtgta atgtctggca ggaagaggtg acttccacgc ggccagaaaa
     1201 acagtggcaa aacacattct gggtcgattc ggctactggc caggttcgtc aaagtcggca
     1261 aatgttaggc gcaggggtta ttcccgtaga aatgacgttt cttaaacccg caccatgaat
     1321 aaattacagt cgtatttcat tgccagcgta ctttacgtaa tgacacccca tgcctttgcg
     1381 caaggaacgg tgactattta tctgcctggc gaacaacaga cactttccgt tggccccatg
     1441 gaaaatgttg tccagttggt gacacaaccg caactgcgcg atcgcctctg gtggcctggt
     1501 gccttgctga ccgactctgc ggcgaaagct aaagcgctta aagactacca acatgtcatg
     1561 gcacagcttg cttcctggga agcagaagct gacgacgatg tggcggcaac gattaaatcc
     1621 gtgcgccagc agttactcaa tctcaatatc actggtcgct taccggtcaa actggaccct
     1681 gattttgtac gagtggatga gaacagcaat cccccgttgg tcggggacta tacgctctat
     1741 accgtacagc gtccggtgac tattactctg ctgggtgcgg tttctggggc cgggcaattg
     1801 ccctggcagg caggccgtag cgttaccgat tatctgcaag atcacccccg ccttgcaggc
     1861 gcggacaaga ataacgttat ggtgattacg cctgagggtg agactgtcgt cgcccctgtt
     1921 gccctgtgga ataaaagaca tgtggaacca ccgcctggca gccagctttg gttgggcttt
     1981 tctgcgcatg tactgcccga aaaatatgct gatttaaatg accagattgt gtctgtttta
     2041 acgcaacgtg ttcctgactg atgaagaaga attcttatct tttaagctgc ctggccattg
     2101 ccgtctccag tgcctgtcat gctgaagtat taacctaccc ggatccgctg ggttcgtcgc
     2161 aatcagactt tggcggcaca ggattgttgc agatgccaaa tgcgcgcatc gcaccggaag
     2221 gtgaattcag cgtcaactac cgggataacg atcaataccg gttctactcc acctccgtgg
     2281 cgctgttccc atggctggaa ggcaccattc gttatacgga tgtgcgcaca cgcaaatata
     2341 gccagtggga agatttcagc ggcgatcagt catacaaaga caaatcattc gattttaaac
     2401 ttcgcctgtg ggaagaaggt tactggctac cgcaagtggc gtttggtaaa cgtgatattg
     2461 ctggtacggg tctgtttgac ggtgagtatc tggtggccag caagcaagcg gggccatttg
     2521 atttcaccct cgggatggca tggggctacg ccggtaatgc gggcaatatt accaacccgt
     2581 tttgccgggt gagcgataaa tattgtcatc gcgcagagtc tcacgatgcg ggcgatatca
     2641 gctttagcga tatctttcgt ggcccggctt ccatctttgg cggcattgag tatcaaacgc
     2701 cgtggaatcc cctgcgtctg aaactcgaat acgatggcaa caattaccag aatgatttcg
     2761 ctggcaaact gcctcaggca agccatttca acgtcggcgc agtttatcgc gctgccagct
     2821 gggcagatct caacctgagt tatgaacgcg gtaacacgtt gatgtttggc ttcacgttac
     2881 ggaccaattt caacgatctg cgccctgccc tgcgcgatac gccaaaaccg gcatatcaac
     2941 ctgcgcctga atctgaagga ttgcagtaca ccacagtagc gaaccaactt accgcgctga
     3001 agtacaacgc aggttttgaa gcaccggaaa ttcagctgcg cgataagacg ctgtatatgt
     3061 ctggtcaaca atacaaatac cgtgattctc gcgaagcggt cgatcgtgcc aaccggattc
     3121 tggtgaataa cctgccgcaa ggcgttgaga agattagcgt gacgcaaaag cgcgagcata
     3181 tggcgatggt gactaccgaa accgacgtag ccagcctgcg caaacagctg gctggtacag
     3241 cgcctggtca atcagagcaa cttcaacaac aacgtgttga agcagaagat ctttctgcct
     3301 ttggtcgggg ctaccgtatt cgtgaagatc gctttagcta ctctttcaac ccaacacttt
     3361 cacagtcgct gggcggcccg gaagatttct atatgttcca gctggggctg atgtccagtg
     3421 cccgctactg gtttaccgac cacctgctgc ttgatggcgg tattttcacc aatatttaca
     3481 acaactacga caagtttaag tcttcgctgt tgcccgcgga ctctaccctg ccccgcgtgc
     3541 gcacgcatat ccgtgattac gttcgcaatg acgtttatct caacaacttg caggcgaact
     3601 actttgccga cttaggcaat ggtttctatg gccaggtgta tggcggttat ctggaaacga
     3661 tgtacgccgg tgtcggttcc gagctgcttt atcgcccgct agatgccagc tgggcgctgg
     3721 gtgtggacgt taactacgtg aagcaacgtg actgggacaa catgatgcgc ttcaccgatt
     3781 attccacgcc aactggtttc gtgacggctt actggaaccc gccgacgctc aatggcgtac
     3841 tgatgaaact tagcgttggg caatatctgg caaaagataa aggggcaacg atcgacgtcg
     3901 ccaaacgctt tgacagcggc gtggcggtag gggtatgggc ggcaatcagt aacgtatcta
     3961 aagatgacta cggcgaaggc ggctttagta aaggttttta tatctcgatt ccgttcgact
     4021 tgatgaccat tggacctaac cgcaaccgcg cggtggtttc gtggacacca ttgacgcgtg
     4081 atggtggaca aatgctgtca cgcaaatacc agctctatcc aatgacggca gagcgagaag
     4141 taccggttgg acaataacct gacgtaattc ataacattac attgactgat aaatataaac
     4201 caatgaaaaa gaacattttt aagttttcgg tactgacatt ggctgttctg tctttgacag
     4261 catgtaccct tgttcctggt cagaatttat cgaccagtaa caaggatgtc attgaactac
     4321 ctgacaatca gtatgacctg gacaaaatgg tcaatatcta tcccgtcacg ccaggattga
     4381 ttgatcagtt gcgcgccaaa ccgattatgt cgcaggctaa cccggagctg gagcagcaaa
     4441 tcgcaaacta tgaatatcgt atcgggattg gtgatgtgtt gatggttacg gtctgggatc
     4501 acccggaact aaccaccccc gcagggcaat accgtagcgc cagtgatacc ggcaactggg
     4561 ttaacgccga tggtgcgatt ttctacccct atattggtcg cctgaaagtg gccgggaaaa
     4621 cacttaccca ggtacgcaac gagattacag cgcgtctcga ttcagtcatt gaaagcccgc
     4681 aagtcgatgt cagcgtcgcg gcgttccgat cgcaaaaagc gtacgtcacc ggcgaagtca
     4741 gcaaatcggg tcagcagccg attaccaata ttcctctgac catcatggat gcgattaacg
     4801 ccgcgggcgg tctgaccgca gatgcagact ggcgcaacgt ggtgctcacc cagaatggcg
     4861 taaagaccaa agtgaacctc tatgcgctga tgcagcgtgg cgacttgcgc cagaacaaac
     4921 tgctgcaccc gggcgatatt ttatttattc cgcgcaacga tgatctgaaa gtcttcgtga
     4981 tgggtgaagt gggtaaacag agcacgctga aaatggaccg cagcggtatg accctggcgg
     5041 aagcgctggg taacgctgag ggtatgaatc aggatgtcgc tgatgcgacg gggatttttg
     5101 ttattcgtgc aacgcaaaac aagcagaatg gcaaaatcgc caatatttat cagctgaatg
     5161 cgaaggacgc atcggcaatg atactcggaa cggaattcca gcttgaacct tacgacattg
     5221 tttatgtcac taccgcgccg ctggctcgtt ggaaccgtgt gatttctctg ctggtaccga
     5281 cgatttccgg ggtgcatgac ctgacagaga cttcacggtg gattcaaaca tggcccaact
     5341 aaaatttaac tcaatcctgg tggtttgtac cggcaacatt tgccgttcgc ccattggcga
     5401 gcgcttgctg cgtaaacgtc tgccgggcgt aaaagtcaaa tcggcaggtg ttcatggtct
     5461 gataaaacac cctgcagatg cgacagcggc agatgttgcc gctaatcatg gcgtatctct
     5521 tgaaggacat gccggacgta agctcactgc tgagatggca cgaaattacg atctgattct
     5581 ggcaatggag tcggaacata ttgctcaggt taccgcaatt gctcccgagg tccgcgggaa
     5641 gacaatgctg tttgggcaat ggctggaaca gaaagagatc ccggatccct atcgtaaaag
     5701 tcaggacgca tttgaacatg tctacggtat gttggagcgc gccagtcagg aatgggcgaa
     5761 gcgcctcagc cgataatttg agtttataaa atacgatgac aactaaaaat atgaatacgc
     5821 caccaggcag cactcaggaa aatgagatcg atctgcttcg tctggtcggc gagttatggg
     5881 atcaccgtaa gtttattatc agcgtgaccg cgttattcac gctgatcgct gtcgcttact
     5941 cgctgttaag cacaccaatt tatcaggcag atactctggt ccaggttgag caaaaacagg
     6001 gcaacgccat tctcagcggc ctgagcgata tgatccctaa ctcatcgccc gagtctgcac
     6061 cggagatcca actgctgcaa tcgcgcatga ttctcggtaa aaccattgct gaactgaatc
     6121 tgcgcgacat agttgagcag aagtattttc cgattgtggg tcgcggctgg gcgagattaa
     6181 ccaaagaaaa accaggtgag ctggcgatca gctggatgca tattccacaa ctgaatggtc
     6241 aggatcagca actgacactc acggttgggg aaaacggcca ctatacactg gaaggtgaag
     6301 agttcaccgt caatggtatg gtcggccagc gtctggaaaa agatggcgtt gcgctgacta
     6361 tcgcggacat taaggccaaa ccaggaacac agtttgtcct gagccagcgt accgaactgg
     6421 aagcgattaa cgcattgcag gaaaccttta ccgttagcga acgcagtaaa gaaagcggga
     6481 tgctggaact taccatgact ggtgatgatc cccagttgat tactcgtatt ctgaacagca
     6541 tcgctaacaa ctatttgcaa cagaatatcg ctcgccaggc ggcgcaggat tcacaaagcc
     6601 ttgaattctt acagcgccag ttacctgaag tgcgcagcga gctggaccag gcggaagaaa
     6661 aactcaacgt ttatcgccag cagcgcgatt cggttgacct taacctggaa gccaaagccg
     6721 ttcttgagca gattgtgaac gttgataatc aactcaatga gctgaccttc cgcgaggcag
     6781 agatctccca gctgtataag aaagatcacc caacttatcg tgcgctgctg gaaaaacgcc
     6841 agacgctgga gcaagaacgc aaacgcctga ataagcgggt atcggcaatg ccttccaccc
     6901 aacaggaagt gttgcgttta agtcgtgacg tagaagcggg ccgtgcggta tatctgcaat
     6961 tacttaaccg ccagcaggag ttgagtattt cgaaatccag tgccattggt aacgtgcgga
     7021 ttatcgaccc ggcagtcact cagccgcagc cagtgaaacc gaaaaaagcg ttgaatgtgg
     7081 tgcttggttt tattcttggc ctgtttattt ctgtgggtgc cgtgctggcg cgtgcgatgt
     7141 tgcgtcgtgg tgtagaagcc ccggaacaac tggaagagca cggcatcagc gtttatgcca
     7201 ctatcccaat gtccgagtgg ctggataaac gcacccgtct gcgtaagaaa aatttatttt
     7261 ctaatcagca gcgccatcgt actaaaaata tccccttcct ggcggtggat aacccggcgg
     7321 attctgctgt ggaagccgta cgtgcgctac gaaccagtct gcatttcgct atgatggaga
     7381 cggagaataa cattctgatg atcaccggtg cgacgccaga cagtggtaaa acgtttgtca
     7441 gttcaactct ggcagcggtg atcgcccagt ccgatcaaaa agtgttattt attgatgccg
     7501 acttacgccg tggttattcg cataacctgt ttaccgtgag taatgaacat ggcttgtcgg
     7561 aatatctggc aggtaaagat gagctcaaca aagtgatcca gcattttggc aaaggaggct
     7621 ttgatgtgat tactcgcggt caggtgccac ctaacccgtc tgaactgctg atgcgcgatc
     7681 ggatgcgtca attactggaa tgggcgaacg accattacga tctggtgatt gtcgatacgc
     7741 cgccgatgct ggcggtgagt gatgccgcgg tcgtggggcg ttctgttggc accagcctgc
     7801 tggttgcgcg ttttggcttg aacaccgcca aagaggtgag tttgtcaatg cagcgtctgg
     7861 aacaggcagg cgtcaatatt aaaggcgcta tcctcaatgg tgtgattaaa cgcgccagca
     7921 ccgcttacag ttacggctat aactattacg gttatagtta ctccgagaaa gagtaaaact
     7981 gaaagtggcc ggatgcggcg tgaacgcctt ttccggtcta cgcattagac agttcttcgt
     8041 ttgtcatcag cctcagagca ttcaggtaac tgaatgctct tttttatgca ttacaaactg
     8101 cacgccggta tgcgtgcttc attcacgatt tgcgtaaaac ctgccaacga acacatgccc
     8161 tgcgcatttc gctcttcaca tcctgccagg gtcagtttca cctctccggg cggcgtattt
     8221 aatgacagcg gcgttttatc acgcatctgc tgtaaagtct ggaagaccag cgaaacctga
     8281 atccactggc tgttatcgct tagccgacgc cagcgttcaa acaccagttc accacctggc
     8341 ggcgtgttat ccggctgacc gggaagcgtc cagttgagct ccagtgcgcc gccgagattt
     8401 gccagattag tatcgtgtcc ggcgataaac agcactgaag tgggtaatgt cacaccatac
     8461 gcctgttttt gcggtggatg gggcgtcaac gctgtcttga tcaaatctaa taacggggtg
     8521 gcgcggctgc gggcaacctc tggcgtgcgt tgtagcaaat aaaattgcgc gttatgcaaa
     8581 cttagcaagg tgttccactg gtgtgaatcg gtgatccttc cccaccccgg ctccggcatt
     8641 ccctgtgctt gttgcaggag aaatatctcc gtcagcattg atgcgaggct taccgcaccg
     8701 gttaatgaga cattgtcggc gctcaccttg agttccgatg gtaatgcctg cgttaatgaa
     8761 cagctttcgt cctgtttctc acgtttaagg cacaagtttg attgcggaaa attaagcacc
     8821 cgttccagtt cgcgaaacgc cgtttgccga tgcccggtaa agtcagcaat tgaccctcct
     8881 gccctgctga ggatcgcgtc agtcacgttc gcgttatcca gttggcaaac gccagttttt
     8941 agaggattaa ataacggatc gggactggac gtatcagcct gggtatgtac ggttattgca
     9001 cagtcaggtg ccagcccggc ggcgaaggct tcgcctgttt tacgggtacg ctcgtcgaca
     9061 tcagcaataa tcgcgacctg accagactgc gggcagccct ttttcgccag caatccgtcg
     9121 gctaccagac gctggcgttg gtaatgtccg agataggcga ttagctcacc accgcgcggt
     9181 gtcagccaac ccagttttac cggccaggtt ggccatgcgt ctggggtgac atcctgcatc
     9241 agttgcgtgg ccttggttgg agcacgcaca ccatgacgac tgacaatcac cacactttcc
     9301 agcttcagct ccggctcact ctgagcgaat gcagattgcg gggttaacgg aatcagaaga
     9361 gataaaaatg ggattaagat cgctttcatc gatatgtttc cgcttttcat atctgacatt
     9421 attgattgcc tgatgcgatg ctaatgcgcc gcatcaggcc tacgttcgtt aacttttcag
     9481 acgcgggtcc agccatacca acacaagggt ggagagcgaa cacatcaaca aaatgccgac
     9541 gaaccaaagt aaataccaca ttgttcctgc tccttagtac aactcgtttt cgttacggcg
     9601 gagagtttct gttgtcatgc gtccccacat tttgtagtag ctccagagag tgtagagcaa
     9661 cacaatgggc aaaaatatca gcacgattac caacataatg ctcagcgtca gctgactgga
     9721 agtgctgtcc cacaacgtca ggctggagat tggactcacg cttgacggca tgacaaaggg
     9781 gaacagcgtg atgcctgccg tgaaaatcac gccaaattgc atcaatgacg ccattaaaaa
     9841 tccccaaccc gggcgaccac gataaatcgc catcaccgtc agcaatgggc agaagaaccc
     9901 cagcagcggg aagatccaca aaacgggcga ttcgacgaaa ttattcatcc aggcaccagg
     9961 tagcactgcc accagtttca ttaacggatt ggaaggaccg ttagcatcct gggcgagcag
    10021 tacaaagcca tcaataccga cccacagcca gtaacccgcc agcaaaaagc acagcatcac
    10081 caacagtgca gcgcgtttgg tggccagctg tgaacgcaga tgaatcacac caacagtttt
    10141 cagttgtaac cagacgccac cttgcaaaat caccatccca aggctgagca atccgcacaa
    10201 taaagggaat ggcgtcagca gttgccagaa gctgccgaga tactccacgc gtaattgcgg
    10261 tgtgaaggca aacggcacgc cgagcaacaa gttgccgaag gcgataccga agactaccgg
    10321 cggcaccaga ctgccgatga ccagaccggc gtcccacatt ttacgccagc gtgcatcggc
    10381 gatttttccg cgataatcaa aggccagcgg gcggaagaac agtgagcaca gcaccaggat
    10441 catcgccaca taaaagccgg aaaacgccgc tgcatacact ctgggccagg cggcaaataa
    10501 tgccccacca gcgaggatca accagacctg gttgccttcc cagtgtgcac caacgctgtt
    10561 tatcactatc cggcgttcat catcattacg cgccaccagc ggcagcagac agccgatccc
    10621 catgtcaaat ccgtcggaga tcataaagac caccaggatc acgccaatca gcagccacca
    10681 gatgaagcgc aatgtttcat aatcaaacat gattttcccc tttacccctg ttgctgcgtc
    10741 ggttgttcac tctgcatcgc gctcggcccc agacgggcat atttctgcat caggtagact
    10801 tcggcgatta agaacagggt gtaaagccct acgatcatga tcagtgagaa agccagttgt
    10861 cctgtggtta aagcggagtg cgcggagtat gtcggtaaga tgtcctgtat cgcccacggc
    10921 tgacgaccaa actcggtcat aaaccacccg gcttcaatcg caatccacgg caacggcaaa
    10981 ctccagagcg ccattttcag cacccagcga tgctggtcga ttttgccacg cagcgtctgg
    11041 acaagcgcaa tcagcatcac cagtagcggc agggaaccac agcccaccat gatgcggaaa
    11101 ctccagaata ccggcgcaac ctgaggtatc gcgccacgca tcgccgcctg gtactgtgcg
    11161 gctgtgacat gattcatatc cggcgcatag cgggagagca acatgccgta gcccaggtca
    11221 ccttccagtt cccggaatgc ctgcaacaca tgcggctcac gattgccttg cgatatttcc
    11281 tgcattaaca gccaggccat acgtccgcgt tgcaagcgtg ggtaggtttc agccatcaaa
    11341 tttttcagac ccggcacggt tttatctaat gagtgagtgg cgaggatccc taacagcgcg
    11401 ggaattttga tggcaaaggc gttacgctct tgatcctgtt ccggccaggc aaccacatgg
    11461 aacggtgcag gagcaggttc cgtttgccac tccccttcca tcgccgccag ttttaccggt
    11521 tgtacttgcg cgacttcata cgcagaactg tctccgagtt gtagcgtgcc gataatcgcc
    11581 agagtaccga agacggaacc gatggcaaac gagcgtaatg caacatcgcg ctcccgtccg
    11641 cgcagtaaat accaggcgct gatcgccata ataaacatgg ccccggtcac gtagcccgcc
    11701 attacggtgt gcacaaattt cacctggctg accgggttaa agaccagctc gctgaagctg
    11761 gtcatctcca tacgtagggt gtcgatatca aaatgcgcac cggtcgggta ttgcatccaa
    11821 ccgttggcat tcaatatcca caatgcagag agatttgaac cgaacgccac cagccacgtc
    11881 accagcaggt gctggtattt attcagccgc tgccagccga agaagaaaag cccgacaaag
    11941 gtggattcga ggaagaaggc cattaatgct tccatcgcca gcggtgcgcc aaaaatatcg
    12001 cccacatagt tggaatagaa tgaccagtta gtaccaaact gaaactccat ggtcaggccg
    12061 gtagccacgc caagagcaaa gttgataccg aagagcttac cccagaagcg cgtcatatcg
    12121 cggtagattg ttttgccggt gaccacgtaa atggtttcca tgatagccag caaaaaaatc
    12181 agccccaggg taaggggtac aaataaaaag tgatacagcg cggtcagagc aaactgccag
    12241 cgcgataaat caatgacatc ccacatgcgc actcctgtag gcgaagagag aaaaataacc
    12301 cgataatgtt agatctccct ctttatcctg aaactctacg accagaggta ttcgccgatt
    12361 gcgatattca taagactgaa agcgatactt accgtctttt tacgtcggtg cagcttccgc
    12421 cagccactga catacctcgc taagccgctg cgcagagtcg accaaatctt ctggcgctgc
    12481 cagcaccact tccggtattg ggcagatttc ataactttcc agtaaaggac cttttaaggt
    12541 gtccgtacag cgtaaatgcc agacatggcg taaccccgtg gcgttgatat agctctcgcc
    12601 atagccaatg gtacgaatct gaatatttcc cggcccacag agacgtgaga gaaaaagccg
    12661 atcagcctcg ctgatgggta attgcgtcag attaatgctg tgcggctgcg cgtcggggtt
    12721 acgtacatgc gccagtaact catgcgccaa cggtaggcca ttcatcaggc catcaatggg
    12781 cggcggtaac agcgaatctg tcggcaagag attttgcgtg gccgcctgcc acaacgccag
    12841 cggcgcgcag ccggcctcca gtttgtcctc cagcaacttt tcgccgcgac gtttgcgcac
    12901 ccgccataat ccgcagaaga tcgcctcctg tatttcactt tcgctgtcgt cagcctgctg
    12961 aatgcgcact gacacttccc cttccccgag aagcgtattg ataaagtgac gatcgtcggc
    13021 gttgagattt aacagatcga ctgccagcac ctcgcccaac ggcggctggc gttgacttag
    13081 ttgatgttgt aactcgttta acagcgcaat cacctgcggg ctgtgagcac attgctccag
    13141 ggccgccata ctcggttcat cattcacctg acagctgatc ggcagtggat tcatgctgaa
    13201 actgtcatcg ttcggttgcg ttcctggccc cagcagatgg aaaaaagttt cgctcatgag
    13261 gcacgctcct gttgtggttc gacaagccca cgcatcaggt ttatcagttc cgctcacggg
    13321 tgaataccat tcagcacacc gcgatagttt ccgccggtaa acactaatgt ggcgggaaag
    13381 cgaaagacgc caaagcgatc tccgatggct tcgctctgtt caaggtcagc aatcgccacc
    13441 tgccatgtgt agccgggaaa ctcgcgcagt aattcgccaa tcattaccgg attatcgctg
    13501 acctctggcg tgcgtttcgg gtcactgctt aataacacca cgccgtctgg cgcttgcgta
    13561 agccagtcgt caagacggaa ttcactaacc ggcgtccagc cgcgcgccag cattcgttgc
    13621 cacaacgcat caaatggcgt gtcgttgctc atccctgctc ctccagtgtc atcctgtact
    13681 ggcgcaagcg aacgccttcg taattttcca tcgacagaca gtcataattg agacagcgcg
    13741 attcattagc cggttgcggc acaattcccc atgcagccag ctgcgccagc gccgcctgtt
    13801 ccgctgcggg cagttgctcc cgtgccagtt cgctcaggct gccgccgtag tcgtcgagca
    13861 ttgcgggttg cagaccgacg agggcaatat gtgctggcag atgtccgcgg atatctgcca
    13921 gcgccagcac ttcggagaaa ctgttctggt gcaggctcat tttcttcgcg ctgagataag
    13981 ccggaatacg ttctccggca taggttcgca gcgttccagg ttccagcccg tagtcaatgg
    14041 catcgagaat caacagatgg ctggcgcttt cgacataccc cagcaagttc agtccctgag
    14101 taccgccatc gacaatctcc acatactcgg gccagtggta atgggcatac aaccgttccg
    14161 ccacccgcac gccgaaacct tcatcggccc acagcaggtt gcccagcccc attaccacaa
    14221 cgcgttgctc gctcatgaac gctccttgtt acttattttg ccaaatttgt ggctacggta
    14281 gccgttgacc atggtggaga tcaccgtgtc gtcggacatg atgtcttcac gcagcgccat
    14341 atagacatga ccgatcacaa acgcgccaat cagccacatc cccagccgat gccagctgtg
    14401 aatgtccatt gagttgcctc ccgtccagta gaaaaattcc accacataac ggaacggcgc
    14461 aaaaatagcg tactggctgt gttcgctgta cagcgcaaag ccggtgatga tcataaagac
    14521 cgacatcagg aaatagccga acatcgccgc ctgggcgatg gggttatggc ctatatcggc
    14581 actcggacgt tttgccagaa cagataccag cggatttcat accacacgcc ctgccaccag
    14641 cttttacgcc ataccggcac gataaacagc tcgcgggagt atcgattgcc aacaaaagcc
    14701 cagtagatcc gcatcagcaa gaccacggta aaaaccatcc cggcgctgaa gtgaattaac
    14761 ctgatgtagc ccatatagaa cagatacgtc gcctcgccgc tgacggaagg tagcggcttg
    14821 ccgataaagt atccggtgac catcaacacc gccatgcata acaccgtcag ccagtgccag
    14881 atgcggactg gcgcttcaaa gacatagtgg ctgacaacgt tgtcgctttt ctgttgcatg
    14941 atgattctcc ttcgctgtta acgcacctgc acggagatca gctcgctacc gtcgtcgccc
    15001 agcacgtgtg ttgaacaggc gaggcacggg tcaaagctgt gcagagtacg caggatctcc
    15061 agcggttgct cggggatcgc cattttggtg ttcatcagcg ccgcttcata agcgccaatc
    15121 tgccctttgg gatcgcgcgg gctggtgttc caggtggtcg gcaccacgca ctggtagaga
    15181 tcaatcttgc catcgcgaat ggcggcccag tggcctaacg ccccgcgcgg cgcttcggta
    15241 aagccgacgc cacggcactc tgtcggccag gttgcaggtt cccatttttc cgtggaggcg
    15301 gtggcaagat tgccgttttt caggttagtc atcagcttgt tgaagaaata ttgcaactta
    15361 cctgcggccc actgtgcttc gtgcgcgcgg cacaaaatgc ggcccaacgt tgactggata
    15421 ccggaaagcg gcaggttcag cgccgacatc atgcgatcga ccgactcaac ggtcgcagca
    15481 tcgcctttgt gataagcgat taacgtgcgt gccagcggcc ccacttccat cgcgttaccg
    15541 cgccagcgcg gcgctttgat ccacgagtag cgttcctgtt cattcagctg ctgaatgttg
    15601 gtatcgctgc ctttgacatc gccggggttg taccacgggt cggtaatgcc atcgaatgga
    15661 tgacgcccga cctgatcgtt gggataacga taccaggcgt ggtcgacaaa ctcctgcacc
    15721 tgctgcggat caaccaaatc cactggcagc acattgttga agtcgccgtt aatcaccgcg
    15781 ccgccaggca tcagcagact tttctcacca aagtcgttgg caatatccgg gaacgcaccg
    15841 tagctgagga cacatttatc agaaagaccc gtgccgattt cgctccacgg tttgttgaac
    15901 tgaccgatgg ctaaggcgtc ggggatcatc acgttgttaa tgaagtccgc cgtgcgggtg
    15961 ataatcgact gcaccaggtt caggcgttcc atattgaccg ccccgaccgc gccgctttcg
    16021 tcaatgttaa tggcgcaagg catcccgccg acaatccagt ttggatgcgg gtttttaccg
    16081 ccaaagaccg cgtggatttt gacaatttca cgctggaaat cgagagcttc gagatagtgg
    16141 gcaaagccca tcaggttagc ttctggcggc agtttgtact gcgggtgccc ccagtagcca
    16201 ttgcggaaga tccccaactg cccgccttca acaaattttt tcaggcggtt ttgtacgtcg
    16261 aagaaatagc caggggatga tttcggccaa gaggagagac tttgcgccag ttcggaggtt
    16321 ttccgcgggt cggctttcag cgcatctaac acatcgatcc agtccatccc ggcaagctga
    16381 tagaagtgca ccagatgatc gtggcaccag agcgttgcca gcataatgtt gcggatgata
    16441 ttggcgttgt ccggcacttt aataccgata gcatcttcga tggcgtaaac cgaagccagg
    16501 gcgtgtacgc cagtacagac gccgcagata cgttcaacga acgcccacgc atcgcgcggg
    16561 tcgcgccctt gtaggatgat ctccagcccg cgaaacatgg tgccgcagga gacggcattg
    16621 gtgatcacat tctgatcgtt aatattcact tcgcagcgca tgtggccttc gatgcgcgta
    16681 atcgggtcga ccaccaggcg gcgtccggca ttattgatgg tgtatccctg agtttcgtac
    16741 tgagtgctca tgcctgttta tcctcattgc ctggctgatg ttcggtttct gtaggttgct
    16801 ggttatgacg tctgcgctgg tcaacggcgc tggcgactgc gtgcacacca acagccgctg
    16861 ccaccacacc aagcgcggtt aaaccgacgg tatcggcggt ggaatgagta cccatttgcg
    16921 gaatatcgac cacgcggctg tagaacgaac cgcaatccca gaaaccattt tccgcacagc
    16981 ccaggcagcc gtgaccagac tggattggga aagaaacgcc atcattccag cgtgtggagg
    17041 aacaggcgtt ataggtggta ggccctttgc agcccatttt gtacaggcag taacctttgc
    17101 gggcagcgtc atcatcccaa ctctggacga actctccggc gtcgaagtgg gcgcggcgat
    17161 agcatttatc gtggattcgc tgaccataga acatcagcgg acggcccatt ctgtcgacat
    17221 ctggcaagcg atcaaaggtc accatgtaag taatgatggc gctcatcaca tccgggatcg
    17281 gcgggcagcc aggtactttg ataatgggtt tgtcggtgat gactttgtcg ataggcgttg
    17341 cctgcgtcgg attgggccac gcggcctgca cgcagcccca ggacgcgcag gttccccagg
    17401 cgataatcgc gctggctccg gcagcggcac gtttgagttt ctcaataaac ggtcgaccgc
    17461 tgctgataca gaacatcccc tgctcgccca gcggcggatt accttctact gcgaggatat
    17521 atttgccatt gtattgcgtg atgatgtctt cgaagacttc ttccgcctgg gttccggcgg
    17581 cagccatcaa agtatcgtca taatcgaggg aaatcaggga aaggatgacg tccttcgcca
    17641 gtgggtgagc ggagcggata aaagattcgg tacagcaggt gcattccaga ccgtggatcc
    17701 ataccaccgg aatgcggggt ttgttctcca gcgcccaggc aatctttggt gccattcccg
    17761 cgcctaatcc cagcgacgtg gcagccagac tacaatattt gagaaagctg cgccgggtaa
    17821 cgccctgacg ccgcatggcc tggtaaaatg tttcctcgtt attcatatcg cacgtctctt
    17881 ctccttgcga caccggcagg acaccgggcg cttatggtcg gcaagcgacg tccctgtagc
    17941 aaggcaaacc atcgcttcac gtgagatgct gaaaacgaaa gctcatcctt ctgcactggc
    18001 gcacgtcgcc agaaagtatt gttaataaag cgtagtgaaa ct
//
LOCUS       DAZOXR010000075        17821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZOXR010000075 DAZOXR010000000
VERSION     DAZOXR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.077"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            94..772
                     /locus_tag="ACHUNN_002242"
                     /pseudo
     CDS             94..772
                     /locus_tag="ACHUNN_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            816..5666
                     /locus_tag="ACHUNN_002243"
     CDS             816..5666
                     /locus_tag="ACHUNN_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR0576611.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6034..7689
                     /gene="lldP"
                     /locus_tag="ACHUNN_002244"
     CDS             6034..7689
                     /gene="lldP"
                     /locus_tag="ACHUNN_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0576612.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7689..8465
                     /gene="lldR"
                     /locus_tag="ACHUNN_002245"
     CDS             7689..8465
                     /gene="lldR"
                     /locus_tag="ACHUNN_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0576613.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8462..9652
                     /gene="lldD"
                     /locus_tag="ACHUNN_002246"
     CDS             8462..9652
                     /gene="lldD"
                     /locus_tag="ACHUNN_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0576614.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9848..10321
                     /gene="trmL"
                     /locus_tag="ACHUNN_002247"
     CDS             9848..10321
                     /gene="trmL"
                     /locus_tag="ACHUNN_002247"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0576615.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10374..11195)
                     /gene="cysE"
                     /locus_tag="ACHUNN_002248"
     CDS             complement(10374..11195)
                     /gene="cysE"
                     /locus_tag="ACHUNN_002248"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0576616.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11275..12294)
                     /gene="gpsA"
                     /locus_tag="ACHUNN_002249"
     CDS             complement(11275..12294)
                     /gene="gpsA"
                     /locus_tag="ACHUNN_002249"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0576617.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12294..12761)
                     /gene="secB"
                     /locus_tag="ACHUNN_002250"
     CDS             complement(12294..12761)
                     /gene="secB"
                     /locus_tag="ACHUNN_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0576618.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12824..13075)
                     /gene="grxC"
                     /locus_tag="ACHUNN_002251"
     CDS             complement(12824..13075)
                     /gene="grxC"
                     /locus_tag="ACHUNN_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0576619.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13217..13648)
                     /locus_tag="ACHUNN_002252"
     CDS             complement(13217..13648)
                     /locus_tag="ACHUNN_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0576620.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13893..15437
                     /gene="gpmM"
                     /locus_tag="ACHUNN_002253"
                     /gene_synonym="pgmI"
     CDS             13893..15437
                     /gene="gpmM"
                     /locus_tag="ACHUNN_002253"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0576621.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15471..16730
                     /gene="envC"
                     /locus_tag="ACHUNN_002254"
     CDS             15471..16730
                     /gene="envC"
                     /locus_tag="ACHUNN_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0576622.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16734..17693
                     /locus_tag="ACHUNN_002255"
     CDS             16734..17693
                     /locus_tag="ACHUNN_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0576623.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17680..17821)
                     /locus_tag="ACHUNN_002256"
                     /pseudo
     CDS             complement(17680..17821)
                     /locus_tag="ACHUNN_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4461 a         4618 c         4669 g         4073 t
ORIGIN      
        1 ctgtcattaa tgtaaagcaa catagatcac caataataca atggacaact ctaccggcat
       61 tgatcgctgg tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt
      121 gctgtcactg tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag
      181 ccagaaagta gtttattgaa acaacaagaa gaacattaaa attgatctac ttgaaaaaca
      241 acaagcacag ctaaagagtc aactggaaac tattcaaaaa caacaaactg gcataataag
      301 cagtacaaag actttaactc acgtaattaa atccgttaaa gatcaacaaa acacttttat
      361 ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaatg gttcggtggc
      421 tttagcgggt cgagtgttat ccattgacgc cacagagaat ggcagtgtta ttcatatttc
      481 actggtcaac ttattaagta cacctatctc aaatattggt tttaatgcga catggggtgg
      541 tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc
      601 aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa
      661 gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca
      721 gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat aaatgcagtt
      781 agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt aaagttatct
      841 ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga
      901 aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt
      961 ttggggcatt ggcgaatgcc gggaatgaca acggccaggg tgttgattac ggtagtggat
     1021 cagctggcga cagctgggtt gctataggca aaggggcgaa agcaaatact tttatgaaca
     1081 ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa tatagctctg
     1141 ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa
     1201 tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg ggccaatact
     1261 caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct atgggggact
     1321 cttccaaagc ggaaggagca aacgccattg ccctgggaaa tgccactaaa gctactgaga
     1381 ttatgagtat tgctcttggc gacaccgcca atgcgtcaaa agcgtattca atggcgctgg
     1441 gagcaagtag cgtcgcatct gaagaaaacg caattgccct ggggcgtagc agtgtagcta
     1501 gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg aacgctattg
     1561 cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat aatgcgaagg
     1621 caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa gtcaatacta
     1681 tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata ggccagggca
     1741 acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg agaggcttaa
     1801 acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt gcgcttggta
     1861 gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc attgcggatt
     1921 tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa
     1981 atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt tatgccatta
     2041 gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat gacggtactg
     2101 ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg gctgcgctcg
     2161 ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac agcgctgctc
     2221 atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc acgatttcag
     2281 cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac gatgtcgaag
     2341 ccaacaccgc caatatcgct actaatacca gcaacattgc cacgaataca gcaagtattg
     2401 ccaccaatac caccaatatc accaacctga cggattccgt tggtgacctt caggctgatg
     2461 ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag gataccacca
     2521 gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat gctgttaacg
     2581 gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat atcgccaata
     2641 acacttccaa tattgccact aacaccacca acatctctaa tctgactgag acggtgacta
     2701 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg gcagctcatg
     2761 gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg actggcagca
     2821 ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg acgaatacca
     2881 ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg
     2941 gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct catggcaaca
     3001 ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc agttccgatg
     3061 ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac ttcggcggca
     3121 atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa
     3181 attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc acgtctctcg
     3241 gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac ggtactaatg
     3301 gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc agttctgacg
     3361 cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg ctggggggtg
     3421 gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt gctaatgctg
     3481 attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac gacgctctgc
     3541 tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga aaagataaaa
     3601 ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga
     3661 ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg ggtggtgacg
     3721 cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg aacgccgagt
     3781 acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccctgctg tgggatgaga
     3841 ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc agcatcatca
     3901 ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac ggttctcagt
     3961 tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag ctcgctggta
     4021 acaccgacgc aacctatatc gaagaaaatg gcgctggtat caactacgtt cgtactaacg
     4081 acaacgattt agcctttaac gatgcaagcg cctctggtgt cggcgctaca gctgtaggtt
     4141 ataacgctgt cgcgtctggt gccagcagcg tagccattgg tcagaacagc agcagcaccg
     4201 ttgataccgg tattgcgctg ggtagcagct ccgtttccag ccgtgtgatt gccaaaggtt
     4261 ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg ttatgacacc actgacggcg
     4321 aactgctagg tgcattgtca attggtgatg acggtaaata ccgccaaatc atcaacgtag
     4381 ctgatggttc agaagcccat gacgccgtta cggttcgcca gttgcagaac gctattggag
     4441 cggtcgccac tacgccaacc aagtactttc acgccaactc aacggcagaa gactcactgg
     4501 ccgttggtga agactcgctg gcaatgggtg cgaaaactgt cgttaatggt aatgcaggta
     4561 ttggtatcgg tttgaacact ctggttctag ctgatgcgat caacggcatt gctatcggca
     4621 gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg taacggttct cagaccaccc
     4681 gtggtgcaca gactggctac accgcctaca acatggacgc accgcagaac tctgtaggtg
     4741 agttttctgt cggtagtgaa gacggtcaac gtcagatcac aaacgtcgca gctggttcag
     4801 cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc gtagcgcaaa
     4861 atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact cgcgttacta
     4921 atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac ttcaagacca
     4981 acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt ggttctggtt
     5041 ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca gaggaagaaa
     5101 atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta gctgccggtg
     5161 ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca ggcggcgtgc
     5221 gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc ggtggtggca
     5281 acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac gacgcggtga
     5341 actacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat cagcggatgg
     5401 ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt atcgcttctg
     5461 caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg gcttctattg
     5521 gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca
     5581 atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa tactccgccg
     5641 cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta agggcaaacg
     5701 ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgccccggg tcaacaatct
     5761 aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc cccacaagaa
     5821 ttggccctac caattcttcg cttatctgac ctctggttca caattcccca attaaaactc
     5881 acatcaatat tgccaataca taacatttag ttaaccattc attgtcatta tccctacaca
     5941 acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca acagactcat
     6001 tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca aaactacgat
     6061 cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct gtttttcttc
     6121 tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtagcaatc
     6181 gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg
     6241 gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc agcagtgttc
     6301 gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc tattctttcg
     6361 ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt
     6421 gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct ggtcggcctg
     6481 ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc gccagtggca
     6541 tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat cgacagcttt
     6601 gagattggcc agatggtggg gcggcagcta ccgtttatga ccattatcgt gctgttctgg
     6661 atcatggcga ttatggacgg ctggcgcggt atcaaggaga cgtggcctgc ggtcgtggtt
     6721 gcgggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg gccggagctg
     6781 ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct caaacgctgg
     6841 cagccagtgc gcgtattccg ttttggtgat ttgggggcgt caaagattga tatgacgctg
     6901 gcccacaccg gttacactgc gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg
     6961 acagctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc atcgggtggt
     7021 gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact ggttgcccgt
     7081 atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt tgactggttc
     7141 tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg gctgaagatg
     7201 aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc tctacccatc
     7261 tactccatcg gcatggtgct ggcgttcgcc tttatctcga actattccgg actgtcatca
     7321 acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc gccgttcctc
     7381 ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct gttcgccgcg
     7441 ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt tgccgccaat
     7501 accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat cgcctgtgcg
     7561 gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg
     7621 atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt aacgtggatg
     7681 attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg
     7741 cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg
     7801 cgatgcaact cggcgtatca cgtaattcac tgcgcgaggc gctggcaaaa ctggtgagcg
     7861 aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt catgacacat
     7921 ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat ccggattaca
     7981 gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg catgcggcaa
     8041 tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca acgctaagtg
     8101 aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct
     8161 cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctcag
     8221 tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa ctgaccgaac
     8281 aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa
     8341 tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa gatcaggctc
     8401 gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg gagaaaaacg
     8461 catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc tgccgccgtt
     8521 cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga
     8581 agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg acttaagcct
     8641 ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac cggtgggttt
     8701 gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg
     8761 cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg cgccagccat
     8821 caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta tgcgtaacgc
     8881 gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg atatgccgac
     8941 accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg cggcaatgcg
     9001 ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg
     9061 tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac tggaagatta
     9121 catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc ttgagtggat
     9181 ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg aagatgcgcg
     9241 cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg gccgccagct
     9301 ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga
     9361 tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc gtatgattgc
     9421 gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg caacagcggg
     9481 ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac
     9541 gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc aggggctggg
     9601 taaagagttg cctacggcac tggctccgat ggcgaaaggg aatgcggcat agtcgtttgc
     9661 gccccctcac cctaaccctc tccctcaggg agaggggacc gttcggcgct gtatgtactc
     9721 cctcactctg aaacgacacc gcactctttt tttctccctc gcccctccgg ggagggccgg
     9781 ggtgagggga aaaggccgca cggttcacca attttctgct atcctgcccc cgcactaagg
     9841 gggcagtatg ctaaacatcg tactttacga accagaaatt ccgccaaata ctggcaacat
     9901 catccgtctt tgcgctaata ccggctttcg tctgcatatc atcgaaccga tgggatttgc
     9961 ctgggacgat aagcgcctgc gccgcgcggg gctggactat cacgagttta ccgccgttac
    10021 gcgtcatcat gactatcgcg cgttcctcga agcagaaaat ccccagcgcc tgtttgccct
    10081 caccacgaaa ggtacgcctg ctcacagcgc cgtaagctat caggatggcg attatctgat
    10141 gttcggcccg gaaacacgcg gcctgccagc gagcattctt gatgccctgc ccgctgaaca
    10201 aaaaattcgc attccgatgg tgccggacag ccgcagcatg aatctgtcca atgcggtgtc
    10261 ggtagtggtg tatgaagcct ggcggcagct gggatatccg ggagcgttgt tgagagatta
    10321 gttactgtat gccggataga tgattacatc gcatccggca cgatcacagg acattagatc
    10381 ccatccccat actcaaatgt atggttaata ccgttgaaat gctggtccat atccattgat
    10441 ggcttatcgc tgtctggttt accgacaata cgagccggaa cgccagcggc ggttgtatgc
    10501 ggcggcaccg gttgcagcac cacggaacct gcgccaatct tcgcgccgcg accaacttca
    10561 atattgccga ggattttcgc gcccgcgcca atcatcacac cttcacgaat tttcgggtga
    10621 cggtcaccac cagatttacc cgtaccgcca agcgtcacag attgcagaat cgatacgtcg
    10681 ttttcaatta ccgccgtttc acccacaacg atgcccgtgg cgtggtcgag cataatgcca
    10741 cggccaattt ttgctgccgg gtgaatatcg acctggaacg tcacagaaac ctggttttgc
    10801 agaaagattg ccaatgcgcg acgcccctga ttccacaacc agtgaccgat gcgataggcc
    10861 tgcaaggcat gaaaaccctt caggtataac aacggggttg agtatttatc gactgccggg
    10921 tcgcgggtac gcaccgcctg aatatcacag gccgcagagg cgatcatttc cgggtcagcg
    10981 gcgtaggctt cttccaccac ttcacggata gcaatagcag gcataattgg cgatgacagc
    11041 ttgttcgcca gcatgtagct cagtgcacta ccaaggtttt cgtgcttgag tagcgtcgcg
    11101 tggtaaaaac tggccagcat tggctcacag tccgccagcg ttctggcttc ggctttaata
    11161 ttgttccaga caatttccag ttcttcacac gacattgctt actccacacg atgagataat
    11221 gaccggcccg ttctgcgcgg gccgggtcat agcggtaaca aaggttccct ggggttagtg
    11281 gctgctgcgc tcgtccttgc gtgcacgacc taataacgtc aatgctgcct cgcgcgcgtt
    11341 ttttccgcaa tataatactt gataaatttc ctcggttatt ggcatttcta cgccgaagcg
    11401 atgcgccagt tcgcggactt ctttcgtatt gcggtagcct tccaccacct gaccaatctt
    11461 ctcctgcgcg ctttgtacat ccatgccctg accgagcatc atgccaaaac ggcggttacg
    11521 cgactggttg tcggtacagg taagcaccag atcgcccagc cccgccatgc ccataaaggt
    11581 ggcaggatcg gcacccagcg ccgcgccaag acgcgacatt tcagccagcc cacgggtgat
    11641 cagtgccgta cgcgcattcg caccaaaacc gataccgtcg gacatccccg cgccaatggc
    11701 aatgacgttt ttcaccgcac cgccaagctg cacaccgatg aaatccgggt tgctgtaaac
    11761 gcggaaactt ttgccacagt gcagcaattg ttggagatca tcggcaaagg tctggtcggt
    11821 agaggccaac gaaatagccg tcggtaaacc tgccgccagt tctttcgcaa acgttgggcc
    11881 agagataacc gccagcggaa tttgatcgcc taaggcctca cgcgccacgt cctgtaacag
    11941 acgtccggtt tccgcttcca gccctttggt cgcccacacc agacgcgcat caggacgcat
    12001 cagcggttta atctggcgca gcacttcacc aaagacatgg ctgggtacga cgacgagaat
    12061 attacggctg gctgccagcg cagtggcgag atcgctttca agatggagcg tatcgggaaa
    12121 aggcacatcg gggagaaacg cggcgttaca gcggtcgcgt tcaagcgttg cgatatgttc
    12181 agggtcatgg ccccagagga caacctcgtg gccatttctt gccagggtaa tggcaagagc
    12241 ggtgccgtac gagccggcac cgatcacagt cattgaagca ttacgttggt tcatcaggca
    12301 tcctgatgtt cttcagtacc ttcgccagcc tgctgctgca aatagttcat gaacagcgca
    12361 tcgaagttaa tcggcgcaag gttcagttgc gggaatgtac cgcgggatac cacgctggtg
    12421 atgcactcac gagcatacgg gaacagaatg ttcgggcagt atgcgcccag gcaatgtgcc
    12481 atctgggtgc cttcgatacc cgcgatggag aaaataccgc cctgctgaac ttcacacagg
    12541 aacgcggttt cttcgcccag ggaggccgtt acggtaacac gcagtaccac ttcgtatacg
    12601 tcatctgcca gttgggaaga tgccgtatcc agatcaagtt taacttctgg ttgccaatct
    12661 ttctggaaaa cgtgcggcgc gttcggcgct tcgaaagaga tatccttggt ataaatacgt
    12721 tggatctgga aagtcatttc agtgttgttt tgttctgaca tgtgtagaaa acccttaagt
    12781 gttgtcctta aatactgcgc aatgtaatgc caacgttcac acgttatttc agcaggggat
    12841 ccagtccacc acgtgcatcc aatgcataca agtcatcaca gccgccaatg tgctgtgcgt
    12901 caataaaaat ctggggaacc gtggtgcgac cgctgcgttt gatcatctct tcacgcttgg
    12961 cggcattgcc atcgatcggc aactcctgaa aactcacgcc cttgctgctc agcagtgctt
    13021 ttgcacgatg gcaatacggg caggtttctt tggtatagat ttcaacattg gccataattt
    13081 agctcctgtt tttttaccct gaaatttcat gttgcagggc ggctgcaaaa tggagtgtcc
    13141 ccagaagctt ataacaataa gtcgctgggg gcctaattgc agctaacggc ctgcatcatg
    13201 aaagacgaca ggtaaattat ttgccgcgca ccagaggcaa gttttcgcca gcccagccag
    13261 caacgccctc tttcagcacg aatacttgcg caaaaccagc tttcgtcagc gcgtttgcag
    13321 gctcctggca ctgcatgcca gaaccgtcta ccacgataac cggtttgtct ttgtgcttct
    13381 caagctcacc aacattgttg gctttgattt cgctcggcaa cagattgata gaacctgcga
    13441 tatggccttt acggaagtca tcacgctgac gtaaatccac aaccacagcg tcttctttgt
    13501 tgatcagacg cgtagcttca ccacgagtaa tcaccttcac tttcgaggtc aggctcttaa
    13561 acgtagtcac aagaaccgcc accagtaacg cgatccaggc gatactcagt atgggatgac
    13621 ggccaacaaa ttgcataatt tcttgcatgg ggggtaacaa ctcccgacgt agtgattaaa
    13681 aaaaccagga aaggagtata cctgcgcggt gcggcaaata cagccagcgc gttaactgga
    13741 atgcaatttt gcggggcacg acgaaaaaaa agcgcaaatc tggcatcggc ttgccgcgag
    13801 cggtcgtata ttttgatctt cagaggctat tttatcgatt cagctgtagt aaaattacgc
    13861 aaattttgac tcttgagtat gaggttgtcg caatgtcggt ttctaaaaaa cctatggtac
    13921 tggtgattct ggatggatat ggctatcgtg aagaacagca ggataacgcc atttttagtg
    13981 ctaaaacccc ggtaatggat gcactgtggg ccaatcgtcc gcataccctg atcgacgctt
    14041 ccggtctgga agtcggtctg cctgaccgtc agatgggtaa ctccgaagta ggccacgtta
    14101 acctgggtgc cggccgcatc gtgtatcagg acctgactcg tctggacgtt gaaatcaaag
    14161 atcgcgcttt ctttgctaat ccagtgctga ctggcgcggt agataaagcg aaaaacgcag
    14221 gtaaagcagt acacattatg ggtctgctct ccgcaggcgg cgtacacagc cacgaagatc
    14281 acatcatggc gatggtagaa ctggcagctg aacgcggcgc agaaaaaatc tacctgcacg
    14341 cattccttga cggtcgcgac accccgccgc gcagtgctga atcctcgctg aaaaaatttg
    14401 aagagaaatt tgctgcgctg ggtaaaggcc gcgtagcgtc catcattggt cgttactacg
    14461 cgatggaccg cgataaccgt tgggatcgcg ttgaaaaagc ctatgacctg ctgactctgg
    14521 cgcagggtga gtttcaggcc gatactgccg ttgctggttt gcaggcagct tatgctcgcg
    14581 acgaaaacga cgagttcgtg aaagcgaccg ttattcgtgc agaaggtcag ccagatgcgg
    14641 caatggaaga cggcgatgcg ctgattttca tgaacttccg tgctgaccgc gcgcgtgaaa
    14701 tcactcgtgc attcgtgaac gctgatttcg atggcttcgc gcgtaagaaa gtggttaacg
    14761 tcgatttcgt gatgctgacc gaatacgccg ctgacatcaa aactgcggtt gcttacccac
    14821 ccgcttccct ggttaacacc ttcggcgagt ggatggcgaa aaacgacaaa actcagttgc
    14881 gtatttccga aaccgaaaaa tatgcccacg ttactttctt cttcaacggt ggcgtagaag
    14941 agtcgttcaa aggcgaagat cgcattctga tcaactcacc gaaagtggct acctacgatc
    15001 tgcaaccgga aatgagctcc gcagagctga ccgaaaaact ggttgcggcc atcaagagcg
    15061 gcaaatacga caccattatc tgtaactatc cgaacggcga catggtaggt cacaccgggg
    15121 tgatggaagc ggcggttaaa gcggttgaag cactggatca ctgcgtggaa gaagtcgcga
    15181 aagcggttga gtccgtgggt ggacaactgc tgatcaccgc tgaccacggt aacgctgaac
    15241 agatgcgcga tccggcaacg ggtcaggcac acacggcaca caccaacctg ccagttccgc
    15301 tgatttacgt tggtgataag aacgtgaaag cggttgcagg cggcaaactt tctgacatcg
    15361 cgccgaccat gttgtcgctg atgggtatgg aaatcccgca agagatgact ggtaagccgc
    15421 tgttcatcgt ggaataatcc ctccccatga ggggaaaggc gattaatacc atgacacggg
    15481 ccgtgaaacc gcgcaggttt gcaatcaggc ccatcatcta cgccagcgtt cttagcgctg
    15541 gcgtattgtt gtgcgccttt tccgcccacg cggatgagcg tgaccaactc aaatctattc
    15601 aggccgatat cgccacaaaa gagcgcgcgg tacgccaaaa gcaacaacaa cgcgcaagcc
    15661 tgctcgcaca attgaaaaag caggaagaag cgatctctga agccacccgt aagctgcgcg
    15721 aaacgcaaaa cacgctcaat caactcaata aacagattga tgagatgaac gcgtcgattg
    15781 ccaaactgga gcagcaaaaa gccgcccagg agcgcagcct cgccgcacaa ctggatgccg
    15841 cattccgtca gggcgagcat accggtattc agctaattct cagcggtgaa gaaagccagc
    15901 gtggacagcg tttacaggct tatttcggct atctcaacca ggcgcgacaa gaaaccattg
    15961 cccagttgaa gcaaacgcgt gaagaagtcg ccatgcagcg tgctgaactg gaagagaaac
    16021 aaagcgagca acaaacgctg ttatatgaac agcgcgccca acaggcgaaa ctgactcagg
    16081 ctctgaacga gcgtaaaaag acgctggcgg ggctggaatc ttccatccag caaggtcagc
    16141 agcagctaag tgaactgcgt gccaacgaat cccgtctgcg taacagtatt gcccgtgcgg
    16201 aagccgcggc gaaagcgcgt gctgaacgag aagcgcgtga ggctcaggca gttcgcgaca
    16261 ggcagaaaga agcgacgcgc aaaggcacca cctacaaacc gaccgaaagc gaaaaatcgc
    16321 tgatgtcccg aactggtggc ctgggtgcgc cgcgtggtca ggcattctgg ccagttcgtg
    16381 gccctacgct gcatcgctat ggcgagcagc tacagggtga attacgctgg aaaggaatgg
    16441 ttatcggtgc ctctgaaggt actgaagtta aagcaattgc cgacggtcgg gtgattctgg
    16501 ctgactggct gcaaggctac ggtctggtgg tggtggttga gcacggtaaa ggcgatatga
    16561 gtctttacgg ctataatcaa agcgcgctgg tgagcgttgg ttcgcaggtt cgtgcgggcc
    16621 agccaattgc actggtgggc agcagtggcg gtcagggcag accttcactc tatttcgaaa
    16681 ttcgccgcca gggtcaggcc gtcaatccac agccgtggtt gggaagataa gttttgtttc
    16741 catttcgtcg taacgttctt gcatttgccg ctctgttggc gctctcctcc cctgtaattg
    16801 ctggcaaact tgccatcgtc attgatgatt ttgggtatcg cccgaacaac gaaaaccagg
    16861 tgctggcgat gccttccgct atctccgtcg ctgtattacc cgattcaccg cacgccagag
    16921 agatggcgac caaagcgcat aacagcggtc acgaagtgtt gattcatctc ccgatggcac
    16981 cgttgagtaa acagccgctg gagaaaaata cgctgcgccc ggagatgagc agcgacgaaa
    17041 ttgagcgcat tattcgtagt gcggtcaata acgtgcccta tgccgtgggg atcaataacc
    17101 acatgggcag caagatgacc tctaacctgt ttggtatgca gaaagtgatg caggcgctgg
    17161 agcgttataa tctttacttc ctcgacagcg taaccattgg taatacccag gcgatgcgcg
    17221 ccgcgcaagg cactggcgtg aaggtgatca aacggaaggt gttcctcgac gattcacaga
    17281 acgaagcgga catccgtgtg caattcaatc gcgcaattga cctagcgcgt cgcaacggat
    17341 caaccattgc cattgggcat cctcaccctt caacggtacg cgtgttgcaa cagatggttt
    17401 ataacctgcc gccagacatt acgctggtga aagccagcag cttgctgaat gaaccgcagg
    17461 ttgatacgtc tacaccgccg aaaaacactg tgcccgacac accgcgtaat ccgttccgtg
    17521 gcgtgaagct gtgcaaaccg aagaaaccga tagaacctgt ttatgctaat cgcttctttg
    17581 aagtattaag cgaaagcatc agccagagca cgctgatcgt ttacttccag catcagtggc
    17641 aaggctgggg caaacagcct gaagcggcga agcttaacgc tagcgtaaat taaaggcgct
    17701 acgcgtaatg cgatggtgcg accgcgtttt gtcgcgccat tgccataagc ggaaagacta
    17761 cagtaacgcc tgataaccca cctttacgct gcgcacattg gtaatcaggc gcttatacat
    17821 a
//
LOCUS       DAZOXR010000076        17777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZOXR010000076 DAZOXR010000000
VERSION     DAZOXR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.078"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(47..1021)
                     /gene="cysB"
                     /locus_tag="ACHUNN_002257"
     CDS             complement(47..1021)
                     /gene="cysB"
                     /locus_tag="ACHUNN_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0576624.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1231..3828)
                     /gene="topA"
                     /locus_tag="ACHUNN_002258"
     CDS             complement(1231..3828)
                     /gene="topA"
                     /locus_tag="ACHUNN_002258"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0576625.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4208..4459
                     /locus_tag="ACHUNN_002259"
     CDS             4208..4459
                     /locus_tag="ACHUNN_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0576626.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="ACHUNN_002260"
     CDS             complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="ACHUNN_002260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0576627.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5764..6522
                     /locus_tag="ACHUNN_002261"
     CDS             5764..6522
                     /locus_tag="ACHUNN_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0576628.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6519..7109
                     /gene="cobO"
                     /locus_tag="ACHUNN_002262"
     CDS             6519..7109
                     /gene="cobO"
                     /locus_tag="ACHUNN_002262"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0576629.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7149..8021)
                     /gene="rluB"
                     /locus_tag="ACHUNN_002263"
     CDS             complement(7149..8021)
                     /gene="rluB"
                     /locus_tag="ACHUNN_002263"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0576630.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8234..9348)
                     /locus_tag="ACHUNN_002264"
                     /pseudo
     CDS             complement(8234..9348)
                     /locus_tag="ACHUNN_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9376..9996)
                     /locus_tag="ACHUNN_002265"
     CDS             complement(9376..9996)
                     /locus_tag="ACHUNN_002265"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0576631.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9993..10874)
                     /gene="rnm"
                     /locus_tag="ACHUNN_002266"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(9993..10874)
                     /gene="rnm"
                     /locus_tag="ACHUNN_002266"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0576632.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11012..11056
                     /gene="trpL"
                     /locus_tag="ACHUNN_002267"
     CDS             11012..11056
                     /gene="trpL"
                     /locus_tag="ACHUNN_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0576633.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11033..11126
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11148..12710
                     /gene="trpE"
                     /locus_tag="ACHUNN_002268"
     CDS             11148..12710
                     /gene="trpE"
                     /locus_tag="ACHUNN_002268"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0576634.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12710..14305
                     /gene="trpD"
                     /locus_tag="ACHUNN_002269"
     CDS             12710..14305
                     /gene="trpD"
                     /locus_tag="ACHUNN_002269"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0576635.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14306..15667
                     /gene="trpCF"
                     /locus_tag="ACHUNN_002270"
     CDS             14306..15667
                     /gene="trpCF"
                     /locus_tag="ACHUNN_002270"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0576636.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15679..16872
                     /gene="trpB"
                     /locus_tag="ACHUNN_002271"
     CDS             15679..16872
                     /gene="trpB"
                     /locus_tag="ACHUNN_002271"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0576637.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16872..17678
                     /gene="trpA"
                     /locus_tag="ACHUNN_002272"
     CDS             16872..17678
                     /gene="trpA"
                     /locus_tag="ACHUNN_002272"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0576638.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4246 a         4725 c         4453 g         4353 t
ORIGIN      
        1 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
       61 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      121 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      181 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      241 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      301 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      361 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      421 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      481 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      541 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagtgaaa tcagcattgc
      601 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      661 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      721 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      781 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
      841 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
      901 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
      961 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1021 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1081 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1141 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1201 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1261 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1321 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1381 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1441 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1501 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1561 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1621 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1681 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1741 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1801 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     1861 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     1921 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     1981 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2041 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2101 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2161 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2221 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2281 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2341 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2401 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2461 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2521 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2581 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2641 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2701 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2761 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     2821 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     2881 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     2941 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3001 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3061 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3121 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3181 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3241 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3301 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3361 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3421 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3481 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3541 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3601 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3661 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3721 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3781 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     3841 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     3901 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     3961 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4021 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4081 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4141 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4201 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4261 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4321 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4381 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4441 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4501 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4561 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4621 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4681 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4741 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4801 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     4861 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     4921 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     4981 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5041 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5101 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5161 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5221 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5281 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5341 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5401 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5461 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5521 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5581 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5641 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5701 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5761 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     5821 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     5881 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     5941 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6001 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6061 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6121 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6181 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttgggcg tcagggacga
     6241 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6301 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6361 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6421 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6481 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6541 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6601 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6661 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6721 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6781 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     6841 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     6901 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     6961 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7021 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7081 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7141 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7201 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7261 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7321 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7381 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7441 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7501 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7561 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7621 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7681 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7741 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7801 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     7861 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     7921 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     7981 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8041 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8101 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8161 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8221 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8281 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8341 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8401 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8461 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8521 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8581 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8641 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8701 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8761 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     8821 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     8881 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     8941 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9001 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9061 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccaccag gctgcatcca
     9121 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9181 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9241 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9301 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9361 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9421 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9481 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9541 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9601 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9661 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9721 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9781 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
     9841 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
     9901 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
     9961 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10021 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10081 agttcaatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10141 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10201 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10261 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10321 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10381 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10441 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10501 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10561 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10621 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10681 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10741 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10801 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    10861 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    10921 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    10981 tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11041 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11101 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11161 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11221 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11281 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11341 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11401 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11461 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11521 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11581 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11641 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11701 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11761 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    11821 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    11881 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    11941 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12001 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12061 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12121 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12181 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12241 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12301 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12361 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12421 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12481 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12541 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12601 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12661 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12721 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12781 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    12841 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    12901 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    12961 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13021 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13081 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13141 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13201 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13261 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13321 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13381 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13441 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13501 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13561 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gctgcctgtg ggctgaaagt
    13621 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13681 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13741 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13801 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    13861 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    13921 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    13981 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14041 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14101 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14161 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14221 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14281 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14341 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14401 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14461 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14521 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14581 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14641 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14701 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14761 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    14821 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    14881 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    14941 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15001 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15061 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15121 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15181 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15241 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15301 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15361 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15421 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15481 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15541 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15601 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15661 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15721 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15781 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    15841 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    15901 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    15961 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16021 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16081 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16141 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16201 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16261 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16321 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16381 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16441 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16501 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16561 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16621 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16681 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16741 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16801 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    16861 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    16921 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    16981 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17041 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17101 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17161 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17221 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17281 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17341 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17401 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17461 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17521 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17581 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17641 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc cccctgccgc cagttccgct
    17701 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17761 ctttttaccg tttcgct
//
LOCUS       DAZOXR010000077        17448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZOXR010000077 DAZOXR010000000
VERSION     DAZOXR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.079"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /locus_tag="ACHUNN_002273"
     CDS             complement(<1..502)
                     /locus_tag="ACHUNN_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0576639.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="ACHUNN_002274"
     tRNA            complement(688..772)
                     /locus_tag="ACHUNN_002274"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="ACHUNN_002275"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="ACHUNN_002275"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0576640.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHUNN_002276"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHUNN_002276"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0576641.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="ACHUNN_002277"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="ACHUNN_002277"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR0576642.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="ACHUNN_002278"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="ACHUNN_002278"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR0576643.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="ACHUNN_002279"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="ACHUNN_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR0576644.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="ACHUNN_002280"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="ACHUNN_002280"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR0576645.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="ACHUNN_002281"
     CDS             7117..8619
                     /locus_tag="ACHUNN_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0576646.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHUNN_002282"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHUNN_002282"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0576647.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHUNN_002283"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHUNN_002283"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0576648.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHUNN_002284"
     CDS             complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHUNN_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0576649.1"
                     /translation="MSIIILATISTTQENK"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="ACHUNN_002285"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="ACHUNN_002285"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0576650.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="ACHUNN_002286"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="ACHUNN_002286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0576651.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="ACHUNN_002287"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="ACHUNN_002287"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0576652.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="ACHUNN_002288"
     CDS             complement(16186..17382)
                     /locus_tag="ACHUNN_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR0576653.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4337 c         4453 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagcatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAZOXR010000078        17342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZOXR010000078 DAZOXR010000000
VERSION     DAZOXR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.080"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            34..663
                     /locus_tag="ACHUNN_002289"
     CDS             34..663
                     /locus_tag="ACHUNN_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0576654.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUNN_002290"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUNN_002290"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0576655.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUNN_002291"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUNN_002291"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0576656.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="ACHUNN_002292"
     CDS             complement(3031..3228)
                     /locus_tag="ACHUNN_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0576657.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUNN_002293"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUNN_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0576658.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUNN_002294"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUNN_002294"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0576659.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUNN_002295"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUNN_002295"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0576660.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUNN_002296"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUNN_002296"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0576661.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUNN_002297"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUNN_002297"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0576662.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUNN_002298"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUNN_002298"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR0576663.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUNN_002299"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUNN_002299"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0576664.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUNN_002300"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUNN_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0576665.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUNN_002301"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUNN_002301"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0576666.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUNN_002302"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUNN_002302"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0576667.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUNN_002303"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUNN_002303"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0576668.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUNN_002304"
     CDS             complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUNN_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0576669.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4065 a         4938 c         4517 g         3822 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agtccaatag gggaaaatct
    17281 ccacggcgct cactttacca gttggtgaga attatccgaa gctgaagttt gtaaattcct
    17341 tc
//
LOCUS       DAZOXR010000079        16997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZOXR010000079 DAZOXR010000000
VERSION     DAZOXR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.081"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHUNN_002305"
     CDS             <1..266
                     /locus_tag="ACHUNN_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0576670.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHUNN_002306"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHUNN_002306"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0576671.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUNN_002307"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUNN_002307"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0576672.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHUNN_002308"
     CDS             complement(1570..3270)
                     /locus_tag="ACHUNN_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR0576673.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQEQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHUNN_002309"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHUNN_002309"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0576674.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHUNN_002310"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHUNN_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR0576675.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="ACHUNN_002311"
     CDS             4828..6600
                     /locus_tag="ACHUNN_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0576676.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHUNN_002312"
     CDS             6593..8374
                     /locus_tag="ACHUNN_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0576677.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUNN_002313"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUNN_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0576678.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUNN_002314"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUNN_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0576679.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUNN_002315"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUNN_002315"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0576680.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHUNN_002316"
     CDS             11336..11908
                     /locus_tag="ACHUNN_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0576681.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHUNN_002317"
     CDS             complement(11939..12250)
                     /locus_tag="ACHUNN_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0576682.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHUNN_002318"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUNN_002318"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHUNN_002319"
     CDS             12629..12982
                     /locus_tag="ACHUNN_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0576683.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUNN_002320"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUNN_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHUNN_002321"
     CDS             complement(14736..15206)
                     /locus_tag="ACHUNN_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0576684.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUNN_002322"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUNN_002322"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR0576685.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHUNN_002323"
     CDS             complement(16044..16262)
                     /locus_tag="ACHUNN_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0576686.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUNN_002324"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUNN_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0576687.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         4056 a         4121 c         4552 g         4268 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc tcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataatt
//
LOCUS       DAZOXR010000080        16857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZOXR010000080 DAZOXR010000000
VERSION     DAZOXR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.082"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHUNN_002325"
     CDS             92..1132
                     /locus_tag="ACHUNN_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0576688.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUNN_002326"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUNN_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0576689.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUNN_002327"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUNN_002327"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0576690.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHUNN_002328"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHUNN_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0576691.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUNN_002329"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUNN_002329"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0576692.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUNN_002330"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUNN_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0576693.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="ACHUNN_002331"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="ACHUNN_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHUNN_002332"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHUNN_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0576694.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="ACHUNN_002333"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="ACHUNN_002333"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0576695.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="ACHUNN_002334"
     CDS             complement(11776..12612)
                     /gene="kduI"
                     /locus_tag="ACHUNN_002334"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0576696.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12899..14080)
                     /locus_tag="ACHUNN_002335"
     CDS             complement(12899..14080)
                     /locus_tag="ACHUNN_002335"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0576697.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14335..15564
                     /locus_tag="ACHUNN_002336"
     CDS             14335..15564
                     /locus_tag="ACHUNN_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0576698.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16024..16656
                     /locus_tag="ACHUNN_002337"
     CDS             16024..16656
                     /locus_tag="ACHUNN_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0576699.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4449 a         4422 c         3895 g         4091 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccactatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat catcaaagac
    11821 ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg
    11881 gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc
    11941 ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata
    12001 aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt tacctggagc
    12061 cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga caaaatattt
    12121 gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc
    12181 cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt
    12241 tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata
    12301 cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg taccggcacc
    12361 gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt
    12421 accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat
    12481 tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa
    12541 aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt ggatgctctg
    12601 tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga
    12661 aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta
    12721 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    12781 aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc
    12841 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    12901 actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca
    12961 gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    13021 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    13081 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    13141 cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    13201 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    13261 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    13321 ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg
    13381 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat
    13441 caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc
    13501 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    13561 actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    13621 cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa
    13681 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    13741 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    13801 ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta ttaggaagac
    13861 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    13921 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    13981 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga
    14041 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    14101 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    14161 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    14221 caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata cttaacctgg
    14281 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    14341 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    14401 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    14461 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    14521 ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca ctactatggc
    14581 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    14641 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    14701 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt
    14761 ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc
    14821 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    14881 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    14941 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    15001 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    15061 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    15121 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    15181 gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    15241 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    15301 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    15361 attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta tgcgatcagc
    15421 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    15481 gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    15541 gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt
    15601 gaaccattcc atttttcata atattcattt gttattattc cgccctatca cttctctcgt
    15661 catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    15721 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    15781 cgatatagat attctcatac catgagatag ttatctggac cacgttcgac catgcaagat
    15841 taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa tgtcttaatc
    15901 agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaagat
    15961 gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt
    16021 aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc
    16081 caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg tttttgtatg
    16141 tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt aacaaaaaac
    16201 cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt tttacgtcta
    16261 ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat agttttagtc
    16321 agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac cgcaatatct
    16381 gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag acaactattt
    16441 attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga attcaccata
    16501 atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct ggatatggat
    16561 atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg taaaataaac
    16621 gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag ccagaatcat
    16681 actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc agtaacccca
    16741 agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca ttacgaaata
    16801 tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta aagtttt
//
LOCUS       DAZOXR010000081        16850 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZOXR010000081 DAZOXR010000000
VERSION     DAZOXR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.083"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            298..1281
                     /locus_tag="ACHUNN_002338"
                     /pseudo
     CDS             298..1281
                     /locus_tag="ACHUNN_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1597..2799)
                     /locus_tag="ACHUNN_002339"
     CDS             complement(1597..2799)
                     /locus_tag="ACHUNN_002339"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0576700.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3031..4530
                     /gene="pitA"
                     /locus_tag="ACHUNN_002340"
     CDS             3031..4530
                     /gene="pitA"
                     /locus_tag="ACHUNN_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0576701.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4774..5109)
                     /gene="uspB"
                     /locus_tag="ACHUNN_002341"
     CDS             complement(4774..5109)
                     /gene="uspB"
                     /locus_tag="ACHUNN_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0576702.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5500..5934
                     /gene="uspA"
                     /locus_tag="ACHUNN_002342"
     CDS             5500..5934
                     /gene="uspA"
                     /locus_tag="ACHUNN_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0576703.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6033..6086)
                     /locus_tag="ACHUNN_002343"
     CDS             complement(6033..6086)
                     /locus_tag="ACHUNN_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576704.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6252..7721
                     /gene="dtpB"
                     /locus_tag="ACHUNN_002344"
     CDS             6252..7721
                     /gene="dtpB"
                     /locus_tag="ACHUNN_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR0576705.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7770..8522)
                     /gene="rsmJ"
                     /locus_tag="ACHUNN_002345"
     CDS             complement(7770..8522)
                     /gene="rsmJ"
                     /locus_tag="ACHUNN_002345"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0576706.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8530..10572)
                     /gene="prlC"
                     /locus_tag="ACHUNN_002346"
     CDS             complement(8530..10572)
                     /gene="prlC"
                     /locus_tag="ACHUNN_002346"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0576707.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10775..11617
                     /gene="rlmJ"
                     /locus_tag="ACHUNN_002347"
     CDS             10775..11617
                     /gene="rlmJ"
                     /locus_tag="ACHUNN_002347"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0576708.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11689..13041
                     /gene="gorA"
                     /locus_tag="ACHUNN_002348"
     CDS             11689..13041
                     /gene="gorA"
                     /locus_tag="ACHUNN_002348"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0576709.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13095..13178)
                     /gene="dinQ"
                     /locus_tag="ACHUNN_002349"
     CDS             complement(13095..13178)
                     /gene="dinQ"
                     /locus_tag="ACHUNN_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0576710.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13206..13379
                     /locus_tag="ACHUNN_002350"
     CDS             13206..13379
                     /locus_tag="ACHUNN_002350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576711.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13918..14271
                     /gene="arsR"
                     /locus_tag="ACHUNN_002351"
     CDS             13918..14271
                     /gene="arsR"
                     /locus_tag="ACHUNN_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0576712.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14325..15614
                     /gene="arsB"
                     /locus_tag="ACHUNN_002352"
     CDS             14325..15614
                     /gene="arsB"
                     /locus_tag="ACHUNN_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0576713.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15627..16052
                     /gene="arsC"
                     /locus_tag="ACHUNN_002353"
     CDS             15627..16052
                     /gene="arsC"
                     /locus_tag="ACHUNN_002353"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0576714.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16186..16482)
                     /locus_tag="ACHUNN_002354"
     CDS             complement(16186..16482)
                     /locus_tag="ACHUNN_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0576715.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16589..>16850
                     /locus_tag="ACHUNN_002355"
     CDS             16589..>16850
                     /locus_tag="ACHUNN_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0576716.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3888 a         4376 c         4273 g         4313 t
ORIGIN      
        1 gattttaagc accgagacta tccggtcttt tactaaataa tggaaaacaa tccatccctg
       61 gaagataatt gtaaaaaaaa caacagaata agaattaatt ttaggataaa tatttattta
      121 tcatggcctt ttaaccggta aaaaatggca ctcggcacag taaaccccgc tatagttatg
      181 ctatgcattt gtctttaatg atatctcgtc gttatattaa caggctgaaa ttatcattaa
      241 tgcacatttc attattagca gggatacctg aggagtttaa agtgaacata tatatcgggt
      301 ggctttgtgc tggaaagttc agggcaaagc gagtatttcg tcgcgggtca tgtgctgatt
      361 tctctggcgg ccatatgcct ggcattattc actaccgcat ttattatcat ttcgcagctc
      421 acgcgcggcg ttaatacgtt ttacaataca ttgttcccca ttattggcta tgcggggtca
      481 attatcacca tgatatgggg ttgggcactg ttagcaggca atgatgtgat ggcagacgag
      541 tttgtcgccg gccatgttat tttcggcgtt ggtatgattg ccgcctgtgt atcgacggtg
      601 gcagcgtcat ccggtcactt tctgctcatt cccaaaaatg cagcggggag caagagcgat
      661 ggaacgccgg tacaggctta ttcttcatta atcggtaatt gcctcattgc cgttcccgtt
      721 ttactcaccc tgctcggttt catttggtct attacgctgt tacgtagtgc tgacataact
      781 ccgcattatg tcgcgggtca cgtattgctt gggttaaccg caatctgtgc ctgtctaatt
      841 ggccttgttg ccacaattgt ccatcaaaca cgtaatacgt tttcaactaa agaacactgg
      901 ctgtggtgtt attgggttat ttttctcggc tcaatcacgg tactgcaggg gatatacgtc
      961 ttagtcagtt ccgatgcaag cgcccgactg gctcccggca ttattcttat ttgcctcgga
     1021 atgatctgtt acagcatatt ctcaaaagtc tggctactgg cactggtctg gagacgtacc
     1081 tgttcgttag ccaacagaat accgatgata cccgtcttca cctgcctgtt ttgccttttc
     1141 ctggcatcat ttcttgcgga aatggcgcag accgacatgg gatattttat tccttcgcga
     1201 gttctggtcg gtttgggagc ggtatgcttt acgttgttct caatcgtttc aatattagaa
     1261 gcgggttctg ctaaaaaata attgcaacgt accggataaa accagcgttg accatttgcg
     1321 taacgctggt ttttcttagg catcatgaaa taacgcagca ttaatgcata gtggttaagg
     1381 ataaaaaaac aacaaagtta ctgtcttttt cagcctgttc atattttata aagatctggc
     1441 cactgggtga gttttcaatc acctttcctt ccaccttata ttaagcctgg agggtttcag
     1501 ttcacgggct cattagaaaa taatcacaag atagccacta tcaaaattag ctcattttta
     1561 atgcggctct aataattttc atctttaggt aacaggtcag gacgacttcg ctgcaatcaa
     1621 atcctgcgca caagcccacg cactcgacca cgcccactgg aagttatagc cccccagcca
     1681 gccggtaacg tccatcactt cgccgatgaa gtacagccca ggcactttgc gcgcttccat
     1741 cgtccgtgaa gagagttcgt tggtgtccac gccgccgagc gtcacttcgg cagtgcgata
     1801 gccttcagtg ccgttgggtt gtacacgcca gtcggtcaat gtgctaatca gtgcctgttg
     1861 gtcacgcacg ttgagctgtt ttagcgaaac attcgggatt tgcccgagtt gctgtaagcg
     1921 ttcaaccaac cgcttcggta gatgaaccgc cagtgtgttt ttcaggctct gattcggatg
     1981 tgcgttacgc tgctcattca ggaaggtttc gagatccaca tccggtagca gattgatact
     2041 gacaaattca cccggttgcc agtagctgga aatctgcaac accgccggtc cagacaagcc
     2101 gcgatgggtg aagagtaagt tctcacggaa aacgatgccg ttttcagcgg taatcacgga
     2161 aggcaccgcc acgcccgcca gcacctgtaa ctcttcgagc aacggtttat gcagagtgaa
     2221 tggcaccaga cccgcacggg tcggcagcac gttgaggcca aattgttcgg caatcttata
     2281 accaaacggc gacgcgccca gccccggcat tgacagccca ccggtcgcca tgaccagctt
     2341 ttcgcaaccg acagtcatgc cgttcagatc aagcgtgaag cctgtttcat cctccgccac
     2401 actcagcact tcgctacgca atctgaaggt cacattgccc ttctcgcact catccaccag
     2461 catgtcgaca atctgctgcg cggagtcatc gcagaagagt tgccctaacg ttttctcgtg
     2521 ccatgcgatg ccgtgtttat tgaccagatc aatgaaatcc cactgggtaa aacgtgcgag
     2581 tgcagactta caaaaatgcg gattctggct cagataagcg cctggttcga cataaaggtt
     2641 ggtaaagttg cagcgcccac cgccagacat aaggattttg cgccctggtt ttttaccatt
     2701 atcgatcagc agaacccggc gtcctgcctg acctgccagc gcagaacaga acatacccgc
     2761 cgcaccagcg cctataataa tggcatcaaa cctttccacg ttgcgctcct cttagaaaaa
     2821 acgggcgtga attgtaaaga ttcctcagtg gtcgcaccag catcaatatt actaaaagga
     2881 agtatttgcc tgaattatat aagataatta ttttttgagt gaaatccata caggaggcaa
     2941 atcaaaaaaa gtctatattt cactttgccc gcgccgcgaa agtcactgat aatgcgccgc
     3001 gttcatgtcc tcaaaatggc gtaacgtcct atgctacatt tgtttgctgg cctggatttg
     3061 cataccgggc tgttattatt gcttgcactg gcttttgtgc tgttctacga agccatcaat
     3121 ggtttccatg acacagccaa cgccgtggca accgttatct atacccgcgc gatgcgttct
     3181 cagctcgccg tggttatggc ggcggtgttc aactttttgg gtgttttgct gggtggtctg
     3241 agtgttgcct atgccattgt gcatatgctg ccgacggatc tgctgcttaa tatgggatcg
     3301 tctcatggcc ttgccatggt gttctctatg ttgctggcgg cgattatctg gaacctgggt
     3361 acctggtact ttggtttacc tgcatccagc tctcatacgc tgattggcgc gatcatcggg
     3421 attggtttaa ccaatgcgtt gatgaccggg acgtcagtgg tggatgcact caatatcccg
     3481 aaagtattaa gtattttcgg ttctctgatc gtttccccta ttgtcggcct ggtgtttgct
     3541 ggcggtctga ttttcttgct gcgtcgctac tggagcggca ccaagaaacg cgcccgtatc
     3601 cacctgaccc cagcggagcg tgaaaagaaa gacggcaaga aaaagccgcc gttctggaca
     3661 cgtatcgcgc tgatcctttc cgctatcggc gtggcgtttt cgcacggcgc gaacgatggt
     3721 cagaaaggca ttggtctggt tatgttggta ttgattggcg tcgcgccagc aggcttcgtg
     3781 gtgaacatga atgccactgg ctacgaaatc acccgtaccc gtgatgccat caacaacgtc
     3841 gaagcttact ttgagcagca tcctgcgctg ctcaaacagg ctaccggtgc tgatcagtta
     3901 gtaccggctc cggaagctgg cgcaacgcaa cctgcggagt tccactgcca tccgtcgaat
     3961 accattaacg cgctcaaccg cctgaaaggt atgttgacca ccgatgtgga aagctacgac
     4021 aagctgtcgc ttgatcaacg tagccagatg cgccgcatta tgctgtgcgt ttctgacact
     4081 atcgacaaag tggtgaagat gcctggcgtg agtgctgacg atcagcgcct gttgaagaaa
     4141 ctgaagtccg acatgcttag caccatcgag tatgcaccgg tgtggatcat catggcggtc
     4201 gcgctggcgt taggtatcgg tacgatgatt ggctggcgcc gtgtggcaac gactatcggt
     4261 gagaaaatcg gtaagaaagg catgacctac gctcagggga tgtctgccca gatgacggcg
     4321 gcagtgtcta tcggcctggc gagttatacc gggatgccgg tttccactac tcacgtactc
     4381 tcctcttctg tcgcggggac gatggtggta gatggcggcg gcttacagcg taaaaccgta
     4441 accagtattc tgatggcctg ggtgtttacc cttccggctg cggtactgct ttccggcggg
     4501 ctgtactggc tctccttgca gttcctgtaa tcgtacgcac caaaacgagc gggtcagctg
     4561 gcccgcttca gattgtgaca tagtgcgctt tgttcatgcc ggatgcggcg tgaacgcctt
     4621 atccggccta caaaaacatg caaattcaat agattgcaga gattatgtag gcctgataag
     4681 cgtagcgcat caggcaattt tgcgtttgta atcagtctcg agcgggtcag tttactggcc
     4741 cgcttttttt atgcgttatt attgctgaag tgcttaatgc caaatcatca atgcaatcag
     4801 gctgaccacc accagaccac acaatgcgct ggtcagaata aactgccgac gcacccgctc
     4861 acagcggcga ataaattcat catcgtgatg atcgcgataa cgttgggcat agatatacca
     4921 aacgagacgc acctgtttgt tgggttggcc atgtgaggta aaaaagcccc ctccatcaac
     4981 atattgatag agcaatggat cgcagttacg cagtaccact aacaacgcgc gtagtgatga
     5041 gaaatagcgc gccatgttaa caatgcaaac gacacataaa gcccaaaata atgcgacggt
     5101 gcttatcata cctcctcccc ggcgacctgc ccgcggagtt ccaccccggg gctaccgctc
     5161 ccgatacgct gccaatcagt taacaccagg tcctggagaa accgcttttg tggtgaccaa
     5221 catccgagcg gctctataga tagtgtagga gatcaggttg ttttttttcc agaaggttaa
     5281 ccactatcaa tatattcatg tcgaaaattt gtttatctaa cgggtaagca aggcggattg
     5341 acggatcatc cgggtcgcta taaggtaagg atggtcttaa cactgaatct ttacggctgg
     5401 gttagccccg cgcacgtagt tcgcaggacg cgggtgacgt aacggcacaa gaaacgctag
     5461 ctggccagtc atcgacaact ttatggaagg agtaacacta tggcttataa acacattctc
     5521 atcgcggtcg atctctcccc ggaaagcaaa gttctggtag agaaagcagt ctctatggct
     5581 cgcccctaca atgcgaaagt ttctctgatc cacgtagatg taaactactc tgacctatac
     5641 accgggctta ttgatgtgaa tctgggtgat atgcagaaac gcatctctga agagacacat
     5701 catgcgctga ccgagctttc cactaatgca ggctacccaa tcactgaaac cctgagcggc
     5761 agcggcgacc tgggccaggt tctggtcgat gcaatcaaga aatacgatat ggatctggtg
     5821 gtttgtggtc accaccagga cttctggagc aaactaatgt cttccgcacg tcagctgatc
     5881 aacaccgttc acgttgatat gctgattgtt ccgctgcgcg acgaagaaga ataatcatcc
     5941 ccccacgacg tgttcctgaa cgcccgcata tgcgggcgtt ttgctttttg gcgcgccttg
     6001 ttacctgatg cagcgtaaac cccttatctg gcctacgatc tgcgtacgca atcaaaatct
     6061 cctgccaata caacatttaa cactatcata ttttccatca ttagtgtgat cagctggtta
     6121 ttttctgttg taatagtgta ttaatctatt caccgcatca atattaagaa tctctgacag
     6181 atgtaaactt tttcgcgcgt tatcccttac gcgttcatac ttttcaggat ggtattggaa
     6241 ggttaataaa tatgaataca acaacaccca tggggatgct gcagcaacct cgcccatttt
     6301 tcatgatctt ttttgtcgag ttatgggagc gattcggcta ctacggcgtg cagggcgtac
     6361 tggcggtttt cttcgttaaa cagcttggat tctcgcaaga gcaggctttt gtcacttttg
     6421 gtgcttttgc tgcgctggtc tatggcctca tttccattgg cggctatgtc ggcgaccacc
     6481 tgctggggac caaacgcacc attgttctcg gagcacttgt gctggcgatt ggctacttca
     6541 tgaccggcat gtcgctactt aagcctgacc tgattttcat cgccctgggg actatcgctg
     6601 tcggtaacgg cctgtttaaa gctaacccag ccagcttgct ttcgaagtgc tatccgccga
     6661 aagatccgcg gcttgatggc gcattcaccc tgttctatat gtcgatcaat atcggctcgt
     6721 tgatagcgtt atcgctggcc cctgtgatcg ctgatagata cggttattca gtcacctaca
     6781 acctgtgcgg ggcggggtta attatcgcat tactggttta catcgcctgt cgtggaatgg
     6841 tgaaagacat tggttctgaa cccgacttca agccgatgag cttcagcaaa ctgttgtacg
     6901 tattacttgg cagcgtggtg atgatcttcg tatgtgcatg gctgatgcac aacgtagaag
     6961 tcgccaatct ggtgctgatt gttctctcca tcgtcgtcac catcatcttc tttcgtcagg
     7021 cattcaagct ggataaaacc gggcgcaata aaatgtttgt cgcctttgtc ctgatgctcg
     7081 aagcggtggt gttttacatt ctctacgccc agatgccaac gtcgctgaac ttctttgcca
     7141 tcaacaacgt gcatcatgaa attctcggct tttccataaa cccggtcagc ttccaggcgc
     7201 ttaacccgtt ctgggtggta ctcgccagcc caatactggc aggcatttac acgcatctgg
     7261 gtaacaaagg caaagacctc tcgatgccga tgaaatttac tctcggcatg tttatgtgct
     7321 cactgggctt tttgacggcg gcagctgcgg gaatgtggtt tgcggatgca caagggctga
     7381 catcgccatg gtttatcgtg ctggtgtatt tattccagag cctgggtgaa ttgtttatta
     7441 gcgcccttgg cctggcgatg attgctgccc tggtgccgca gcatttgatg ggctttattc
     7501 tcgggatgtg gttcctgacg caggctgccg cattcttgct gggcggctat gtggcaacat
     7561 ttaccgcagt gccggacaac attaccgatc cgcttgagac gttacccgtc tataccaacg
     7621 tgtttggtaa gattggcctg gttacgctgg gcgttgcagt agtgatgctg ttgatggtgc
     7681 cgtggctgaa acgcatgatt gcgacgccgg aaagccatta attattcttg cagaaagcag
     7741 ggtagcgtta tcgctaccct gctttacttt tacacgggcg tacctgcata gatatcaaag
     7801 cgatgccctt tagtcacaac cgcgtttggc gtggcgacat tcgctagcgg tggcgcgtag
     7861 tccgggcgct ttactaccac gcgtttggtc gccagcaagc gtgcaggctc cagcaatcca
     7921 tcggcatcaa gatccggccc caccagcgac tgaaagacac gcatctcttt tttcaccagc
     7981 gcgcttttct gcttatgcgg gaacatcggg tcgagataga ccacctgcgg gcgtggggta
     8041 atatcggtta gcgccgtcag gctggaggcg tgaattaact gcaaacgctc ctgcaaccag
     8101 ccgccgattt ccgcatccgc ataaccacgc gccaggccgt cgtcgagcag cgcggcaaca
     8161 actggattac gctccagcat ccgcacgcgg caaccgactg aagccagcac aaaggcatcg
     8221 cgccccagtc ctgcagtggc atccaccaca tccggcaaat aatcgccttt aatgcccacc
     8281 gctttcgcca ccgcctcacc gcgaccaccg ccgaatttgc gtcggtgcgc catcgctccg
     8341 ccaacaaaat caacaaagat gccgccaagt tttggctcat cacgcttgcg caattccaga
     8401 tgttccggcg ttaacaccag cgccatcagg ttgtcttcat cgtgctccag cccccagcgg
     8461 gccgccagaa cagataaggc gccgtctccg gcgcctgttt catcaattaa gcagattttc
     8521 actgaatgat cagcccttaa tgccgtaatg ctccagcatc gcatccagct gcggttcacg
     8581 accacggaag cgtttgaaca gatccatcgg ctcttctgaa ccgccacggc tcagaatgtt
     8641 gtcgaggaac gactgcccgg tttcacggtt gaaaatgccc tcttcctcaa agcgcgagaa
     8701 agcatctgcc gccagtacgt cagcccacag gtagctgtag taacctgccg cataaccacc
     8761 ggcgaaaata tggctgaaag cgtgcgggaa acggccccag gacggagatg gcaccacggc
     8821 aaccagtttc ttgatttctg ccagagtttc gaggattttt gccccctgat ccgggcggaa
     8881 ctcggcatga aggcggaaat caaacaggcc gaactccagc tgacgcagaa taaacagcgc
     8941 cgcctggtag ttcttcgccg ccagcatttt atccagcaac tctttcggca gcggttcgcc
     9001 ggtttcatag tgaccagaga taaacgccag cgcctccggc tcccagcacc agttttccat
     9061 aaactgactc ggcagttcga ccgcatccca cggcacaccg ctgataccgg aaacaccagc
     9121 ggtttcgatg cgggtcagca tatggtgcag gccgtgaccg aactcgtgga acagggtgat
     9181 cacttcgtcg tgagtgaaca gcgccggttt accatttacc gggcggttga agttacaagt
     9241 caaatacgcg accggttttt gcagagaacc atcagcttta cgcatctggc ctacgcagtc
     9301 atccatccac gccccgccgc gcttgttttc acgggcatac agatcgaggt agaagctgcc
     9361 gcgcagttcg ttattttcgt catacagttc gaagaaacgt acatccggat gccagacatc
     9421 aacctcttta cgctctttag cggtgatgcc gtaaatacgt ttcaccactt caaacaggcc
     9481 gttaaccgat ttgttttccg ggaagtacgg acgcagctgc tcgtcgctga tgctgtagag
     9541 gtgctgtttc tgtttttcgc tgtagtaagc gatatcccac ggctgcaact catcgacacc
     9601 aaattcggct ttagcgaagg cacgcagttg ctccagctct ttttcgcctt gcggacgcgc
     9661 gcgttttgcc agatcggtta agaaatccag cacctgctgc gggttttctg ccattttagt
     9721 ggcaagggat ttaaaggcgt agttttcaaa gcccagcagt tgcgccagtt cgtgacgcag
     9781 cgcgaggatc tcttccatca ccttgctgtt atcccactta ccggcgttcg ggccttgatc
     9841 ggaggcgcgg gtgctgtaag cgcgatacat ctcttcgcgc agagcctggt tgtcgcagta
     9901 ggtcattacc ggcaggtagc ttgggatatc cagcgtcagc aggtagcctt ccagctcttt
     9961 cgcttcggcc tgggcttttg ccgcagccag cgcgctttct ggcatccccg ccagccccgc
    10021 ttcgtcggta acgagtttgg tccagcccat cgtcgcatcg aggacgttgt tgctgtactg
    10081 gttgcccagt tcagaaagac gggtagcaat ttcgccgtaa cgctgctgtt tctctttcgg
    10141 cagacctatg ccagagagtt caaaatcacg cagggcgtta tcaaccgctt tcttctgcgc
    10201 cgtgttcagc gtggcgtaat gatcgccatc gcgcaggtcg cggtacgctt tatacagccc
    10261 ttcatgttgc cctacccagg tgctgtattc cgacagcagc ggcagggttt gctcgtaggc
    10321 ttcacgcagt tccgggctat ttttcaccga gttcaggtgg ctgaccgggg agaagatacg
    10381 ccccaacaca tcgtccactt ccgccaacgg ctggcagaga ttttcccagg tgtacggtgc
    10441 cccttgcgct actacgcgcg ccacattttc gcggcaatcg ttcagcgcct gagtcacggc
    10501 tggaacgaca tgttccggga gaattttaga aaacggaggc aattcaaagg gagtcagtaa
    10561 cggattcgtc attcgcgcag tcctggttaa agaggttaag gaagcgctca acaggcgctt
    10621 tacataatgt gtgtagcatg gggttaagtg tagtgaattt caatgagaaa cgttacgctt
    10681 tcgcggcggc ggcctctttt cggtatactg tcctgatacg cttttgtgcg ccccgaatac
    10741 gggccgattt ttacttaccg gaacaccttt acccatgctc agttatcgcc acagctttca
    10801 cgctggcaac cacgccgacg tccttaaaca taccgttcag agcctgatca tcgagtcgct
    10861 gaaagagaaa gataaaccgt ttctctatct cgatacccac gcaggggccg ggcgttatca
    10921 gttaggcagc gaacatgccg agcgtaccgg cgaatatctc gaaggtatcg cccgtatctg
    10981 gcagcaggac gatttgcccg cagaactgga ggcgtacatc aatgtggtaa aacacttcaa
    11041 ccgtagcggt cagttgcgtt actaccccgg gtcgccgttg attgctcgcc agctactgcg
    11101 cgaacaagac agcctgcaac tgaccgaact gcacccgagc gattacccgt tgctgcgttc
    11161 tgaatttcag aaagacagcc gtgcgcgcgt cgaaaaagcc gacggtttcc agcagcttaa
    11221 ggccaaactt ccgccggttt cccgccgtgg tttaatcctt atcgacccgc cgtatgaaat
    11281 gaaaaccgac tatcaggcgg tggtcagcgg gatagcagaa ggttacaaac gtttcgccac
    11341 tggtacttac gcactgtggt atccggtggt gctgcgtcag caaattaagc gcatgatcca
    11401 cgatctggaa gcgaccggta ttcgcaaaat tctgcaaatt gaactggcgg tactgccaga
    11461 cagcgatcgc cgtggcatga ccgcttccgg catgattgtg attaacccgc cgtggaaact
    11521 ggaacagcag atgaataacg tgctgccgtg gctgcacagc aaactggttc cggcaggcac
    11581 cgggcacgcc accgtaagct ggatcgtgcc ggagtaattg cagccattgc tggcacctat
    11641 tacgtctcgc gctacaatcg cggtaatcaa cgataaggac actctgtcat gactaaacac
    11701 tatgattaca tcgccatcgg cggcggcagc ggcggtatcg cctccatcaa ccgcgcggct
    11761 atgtacggcc agaaatgcgc actgattgaa gccaaagagc tgggcggcac ctgcgtgaac
    11821 gtcggctgcg tgccgaaaaa agtgatgtgg cacgcggcgc aaatccgtga agcgatccat
    11881 atgtacggcc ctgattatgg tttcgatacc accatcaaca agttcaactg ggaaacgttg
    11941 atcgccagcc gtaccgccta tatcgaccgt attcatactt cctatgaaaa cgtgctcggt
    12001 aaaaataacg ttgatgtaat caaaggcttt gcccgcttcg ttgatgccaa aacgctggag
    12061 gtaaacggcg aaaccatcac ggccgatcat attctgatcg ccacaggcgg tcgtccgagc
    12121 cacccggata ttccgggcgt ggaatacggt attgattctg atggcttctt cgcccttcct
    12181 gctttgccag agcgcgtggc ggttgttggc gcgggttaca tcgccgttga gctggcgggc
    12241 gtgattaacg gcctcggcgc gaaaacgcat ctgtttgtgc gtaaacatgc gccgctgcgc
    12301 agcttcgacc cgatgatttc cgaaacgctg gtcgaagtga tgaacgccga aggcccgcag
    12361 ctgcacacca acgccatccc gaaagcggta gtgaaaaatg ccgatggtag cctgacgctg
    12421 gagctggaag atggtcgcag tgaaacggtg gattgcctga tttgggcgat tggtcgcgag
    12481 cctgccaatg acaacatcaa cctagaagcc gctggcgtta aaactaacga aaaaggctat
    12541 atcgtcgtcg ataaatatca aaacaccaat attgaaggta tttacgcggt gggcgataac
    12601 acgggtgcag tggaactgac accggtagca gttgcagcgg gtcgccgtct ctctgaacgc
    12661 ctgtttaata acaagccgga tgagcatctg gattacagca acattccgac cgtggtcttc
    12721 agccatccgc cgattggtac tgttggttta acggaaccgc aggcgcgcga gcagtacggc
    12781 gacgatcagg tgaaagtgta taaatcctct ttcaccgcga tgtataccgc cgtcaccact
    12841 caccgccagc cgtgccgcat gaagctggtg tgcgttgggc cagaagagaa gattgtcggt
    12901 attcacggca ttggctttgg tatggacgaa atgttgcagg gcttcgcggt ggcgctgaag
    12961 atgggcgcaa ccaaaaaaga cttcgacaat accgtcgcca ttcacccaac ggcggcagaa
    13021 gagttcgtga ccatgcgcta aatgttaaag ggctaagagt agtgtgctct tagcccttaa
    13081 ttacgtttcc gctatcagtt cagaagctga agcagaaagc ggatcagttc cagcagcgca
    13141 attaacgctc caagaacgat gattgcttta tcaatcaccc gttttctcca tgcgatggag
    13201 tgagaatgca tccgcttact catccactgc ctgtcacggc gcatgtctca ttgttagatg
    13261 acgactatct cactccggcc agagcatcag ttaacggcac cacccgtact tctgaccagg
    13321 actttgaaag cgtttatgcg cattaccaga gtgaaaatgc ctcagagcta actggataat
    13381 catacagtac attcaggtta taaaaccagc atgtccttgc aatagtttca gtatggtatt
    13441 agcattgatg cgttagatga tgactatctc actccagcca gagccaccaa ctcagggctg
    13501 aagagtaaaa aaaccgacgc aaagtcggtt tttttacatc cggattcgga caaggcttaa
    13561 tatgacgatg acccagtgaa agtatataaa tcgtcattgc gatatatacc gaagtgcccc
    13621 ctccgccagc tgaagaaatc gctaattctt gcaatgttag ccactggcta atagtattga
    13681 accgttagat gtgggcattc tcactccagc cagagccacc aactcaggct gaagagtaaa
    13741 aaaccgacgc aaagtcggtt tttttacgtc ctgattcaaa ccgcctttca aatgaatagc
    13801 caactcaaaa ttcacaccta ttaccttcct ctgcacttac acattcgtta agtcatatat
    13861 gtttttgact tatccgcttc gaagagagac actacctgcc acaatcagga gcgcaatatg
    13921 tcatttctgt tacccatcca attgttcaaa attcttgctg atgaaacccg tctgggcatc
    13981 gttttactgc tcagcgagct gggagagtta tgcgtctgcg atctttgtac tgccctcgat
    14041 cagtcgcagc ccaagatctc ccgccacctg gcattgcttc gtgaaagcgg gctattgctg
    14101 gaccgcaagc aaggtaagtg ggttcattac cgcttatcac cgcatattcc agcatgggcg
    14161 gcgaaaatta ttgatgaggc ctggcgatgt gaacaggaaa aggttcaggc gattgtccgc
    14221 aacctggctc gacaaaactg ttccggggac agtaagaaca tttgcagtta aaaatttagc
    14281 taaacacata tgaattttca gatgtgtttt atccgggagg cattatgtta ctggcaggcg
    14341 ctatctttgt cctgaccatc gtattggtta tctggcagcc gaaaggttta ggcatcggct
    14401 ggagtgcaac gctcggcgca gtactggcgt tagttacggg cgtggttcat ccgggtgata
    14461 ttccggtggt gtggaatatc gtctggaacg cgacggctgc gtttatcgcc gtcattatca
    14521 tcagcctgct gctggatgag tccggctttt ttgaatgggc ggcgctgcac gtctcacgct
    14581 ggggtaatgg tcgtggtcgc ttgctgttta cctggattgt cctgctcggt gctgccgttg
    14641 ccgccctgtt tgccaatgat ggcgcggcgc ttattttgac gccgattgtc atcgccatgc
    14701 tgctggcttt aggattcagt aaaggcacga cgctggcgtt cgtgatggcg gccggattca
    14761 ttgccgatac cgccagcctg ccgcttattg tctccaacct ggtgaatatc gtttccgctg
    14821 atttctttgg cctcggcttt cgcgaatacg cctcggtgat ggtgccggtg gatatcgccg
    14881 cgattgttgc cacgctggtg atgctacatc tctattttcg caaagatatt ccgcagaact
    14941 acgatatggc gctgctgaaa tctcccgcag aagcgatcaa agatcctgct acgttcaaaa
    15001 ctggctgggt tgttttactg cttctgctgg tgggattttt cgtcctggaa ccgctcggca
    15061 ttccggtgag cgccattgca gctgtgggcg cgctgatatt atttgtcgtc gctaaacgcg
    15121 gtcatgcgat taatacgggt aaagtcctgc gcggtgcccc ctggcagatt gtcatcttct
    15181 cgctcggcat gtatctggtg gtttatggcc tgcgcaatgc cggattaacg gaatatcttt
    15241 ctggcgtact caacgtgctg gcggataacg gcctgtgggc cgcgacgctc ggcaccggat
    15301 tcctcaccgc cttcctctct tctattatga acaatatgcc gacggtactg gttggcgcgt
    15361 tgtccattga tggcagcacg gcatctggcg ttatcaaaga agcgatggtt tatgccaatg
    15421 tgattggctg cgatttggga ccgaaaatta ccccaattgg tagcctggct acgctactct
    15481 ggctgcacgt actttcgcag aagaatatga ctatcagctg gggatattac ttccgtacag
    15541 ggattatcat gaccctgcct gtgctgtttg tgacgctggc tgcgctggcg ctacgtctct
    15601 ctttcacttt gtaatgagat actgatatga gcaacattac catttatcac aacccggcct
    15661 gcggcacgtc gcgtaatacg ctggagatga tccgcaacag cggcacagaa ccgaccatta
    15721 tccattatct ggaaactccg ccaacgcgcg atgaactggt caaactcatt gccgatatgg
    15781 ggatttccgt acgcgcgctg ctgcgtaaaa acgtcgaacc gtatgaggag ctgggccttg
    15841 cggaagataa atttactgac gatcggttaa tcgactttat gcttcagcac ccgattctga
    15901 ttaatcgccc gattgtggtg acgccgctgg gaactcgcct gtgccgccct tcagaagtgg
    15961 tgctggaaat tctgccagat gcgcaaaaag gcgcattctc caaggaagat ggcgagaaag
    16021 tggttgatga agcgggtaag cgcctgaaat aaagcggcga tatccactgc cgatagttgg
    16081 taaagtgcgc tttgtttatg tcggatgcga cgctaacgcg tcttatccga cctacaaaac
    16141 cgtgcaaatt caatatatgg catgaaatat gtagagcgta gcgcatcagg caattttgct
    16201 atttacgcag cactcctcgc gtaagaattc aattatcgct tccagcattg cgtattgcga
    16261 aacacacatc agcgtccgac tctcacggtt ttgtttgacc agtcctaccg ataccagtgc
    16321 tgatatatgg tggctgagtg tggagccagg tatcccaagc tgcttttgca actcaccgac
    16381 cggcaaccct tgttccccgg ctttgacaag atgtttaaat atgaacaaac gggtcgggtg
    16441 acctagctct ttcagcgctt tagcgacttc ttccaactgc atatgacacc cctctgtctg
    16501 gttgatattt cgataatacc agaaatatct tgatctgaat ttcatttcga ttattctgga
    16561 aatatagatt tatccaatga ggttacgtat gagtagttgg cttgcgatgc tgcaagatgc
    16621 cgcagagatg tttgtgtttc tcgccgtcga gctttcgttg ctgtttatag tgattagtgc
    16681 cggtgtcagc ctgataagac aaaaggtgcc agaccataaa atccagcaga tgatgggggc
    16741 cagaaaaggg agaggctatc tcctggctgc tctgttggga gccgttaccc cgttctgtag
    16801 ttgctcgaca atacccatgt tacgtggatt gttgtcagcg aaagccgggt
//
LOCUS       DAZOXR010000082        16564 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZOXR010000082 DAZOXR010000000
VERSION     DAZOXR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.084"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(133..285)
                     /locus_tag="ACHUNN_002356"
     CDS             complement(133..285)
                     /locus_tag="ACHUNN_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0576717.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(550..1284)
                     /gene="cysH"
                     /locus_tag="ACHUNN_002357"
     CDS             complement(550..1284)
                     /gene="cysH"
                     /locus_tag="ACHUNN_002357"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0576718.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1359..3071)
                     /gene="cysI"
                     /locus_tag="ACHUNN_002358"
     CDS             complement(1359..3071)
                     /gene="cysI"
                     /locus_tag="ACHUNN_002358"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0576719.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3071..4870)
                     /gene="cysJ"
                     /locus_tag="ACHUNN_002359"
     CDS             complement(3071..4870)
                     /gene="cysJ"
                     /locus_tag="ACHUNN_002359"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0576720.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5189..5551
                     /gene="queD"
                     /locus_tag="ACHUNN_002360"
     CDS             5189..5551
                     /gene="queD"
                     /locus_tag="ACHUNN_002360"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0576721.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5629..6900
                     /locus_tag="ACHUNN_002361"
     CDS             5629..6900
                     /locus_tag="ACHUNN_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0576722.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6891..7151
                     /locus_tag="ACHUNN_002362"
     CDS             6891..7151
                     /locus_tag="ACHUNN_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0576723.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7168..7743
                     /locus_tag="ACHUNN_002363"
     CDS             7168..7743
                     /locus_tag="ACHUNN_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR0576724.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7891..8751)
                     /locus_tag="ACHUNN_002364"
     CDS             complement(7891..8751)
                     /locus_tag="ACHUNN_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0576725.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8748..9527)
                     /locus_tag="ACHUNN_002365"
     CDS             complement(8748..9527)
                     /locus_tag="ACHUNN_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0576726.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9505..10914)
                     /locus_tag="ACHUNN_002366"
     CDS             complement(9505..10914)
                     /locus_tag="ACHUNN_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0576727.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10936..12390)
                     /locus_tag="ACHUNN_002367"
     CDS             complement(10936..12390)
                     /locus_tag="ACHUNN_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0576728.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12460..13276)
                     /locus_tag="ACHUNN_002368"
                     /pseudo
     CDS             complement(12460..13276)
                     /locus_tag="ACHUNN_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13594..14871
                     /locus_tag="ACHUNN_002369"
     CDS             13594..14871
                     /locus_tag="ACHUNN_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR0576729.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14898..16383
                     /locus_tag="ACHUNN_002370"
                     /pseudo
     CDS             14898..16383
                     /locus_tag="ACHUNN_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3940 a         4359 c         4166 g         4099 t
ORIGIN      
        1 taattttcat ccgtaatata tatgaataat aaacataggc ataatattca cataaataat
       61 ataaattaaa aaagggtgca ggagaccaaa ccatcacaat tcagtacgcg gggttttacc
      121 cccgcgtggt ggctacttct tcgattcgta agcgagaact gccttaaact ccataccacg
      181 ttctctgata ctcaactcac acaacgagtc gcctaccatc agcgtgaatc ccagtactgt
      241 aaaacacagc actatgattg ccacaagggc atattttgtc agcatgtctt gcctccctgc
      301 aaagaagaga ctaacattcg aattgtttgg gttcgaacgc tggcctcagg ttgatagaaa
      361 tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg cttaacgcca gacagcctca
      421 agcacccgac gccattctat acctgataat tcttccagcg gcgttcacgt ttaaaatcag
      481 gaatactgct cgcaaaataa aaacgccccc atcatttctg acagaggcgt ttaatttatc
      541 cggcaatatt tacccttcgt gcaatccaca ttcccttttt aagccaaaga aacgtgtttc
      601 ttcttccgac atgccaggtt cccatttacg ggttgtatgg gtatcaccga ccgataaata
      661 tccttcatcc cataatgggt gatatttcag gccatgtttt tgcaggtact gataaatagt
      721 tcggttatcc cagtcgataa tcggcagcac tttaaatacg ccacgctgaa ttgccagcac
      781 cggtaaattg gcgcgactgc cggattgttc acggcgcagg ccagcaaacc aggtttgcgc
      841 gttaagttct ttcagagccc ggttcatcgg ttcgactttg ttgatgtcat tgtacttttc
      901 aatgccttca acgccctgct cccacagttt tccgtagcgt gcttcctgcc aggctgcgct
      961 ttcggtagca cggtacactt tcaggttgag cttgagtttg tccgttaact cgtcaataaa
     1021 gcggtaggtt tccgggaaca agtaacccgt atcggtgagg atcaccggaa tatccgggcg
     1081 aatttgattc accagatgca ggctcaccgc cgcctgaatg ccaaagctgg aagaaagtac
     1141 atattcaccg ggcagattat ccagcgccca ggctacgcgg ccttcagcgt ccagtttttc
     1201 cagttcggcg ttagtttccg ccagcgccag aatacgatct accttcggca gttcgttcag
     1261 ggcgtttaga tcgagtttgg acatagattc ctcactgttt gccttgcctg atgcgacgct
     1321 cacgcgttct tatcaggcct acaagaccgg gctgatggtt aatcccacaa atcgcgcgcc
     1381 ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa agtcgccgaa gccttcaccc
     1441 gcttcgcgct ctttcgccca gcgccctatc agttcatcaa gcgacgccag gatttccggc
     1501 tcggtgatgt tttctttata catccgtggg atacgtgtcc caatgcggtt gccgccaaga
     1561 tgcaggttgt agcgacccgg cgctttaccc accaggccca cttccgccag catcgcgcga
     1621 ccacaaccgt tcgggcagcc tgttacacgc atcacgatat gctcatcgct gacaccatgt
     1681 ttcgccatta aattatcgat gttgtcgata aaagacggca ggaaacgctc tgcttccgcc
     1741 atcgccagcg ggcaagtcgg gaatgacacg caggccatcg agttttcacg ctgcggcgtg
     1801 acggcattca ttaacccgct ctctttggcg atcttctcga tcttcgcttt ctcgctttcc
     1861 ggtacaccgg tgatgatcag attctggttc gccgtaatgc ggaaatcgcc tttgtggatc
     1921 ttcgcgatct ccagcaggcc ggttttcagc ggacgccccg gataatcaag gatgcgacca
     1981 ttttcgataa acagcgtcag gtgccagtta tcatcaatgc ccttaaccca gccaatacga
     2041 tcgcctcgtc cggtgaactc atatggacgg atcggttcaa atttgatccc cgcgcgacgc
     2101 tccacttccg ctttaaacgt ctcaaccccc acgcgctcca gcgtgtattt ggttttggca
     2161 tttttacgat cggttcggtt accccagtca cgctgagttg tcacgacggc ttcggccacc
     2221 gccagcgtat gctccagcgg cagatagcca aactcgctcg ccgtgcgggc gtaggttttc
     2281 ttgttgccgt gttcgataga aagcccaccg cccaccagca ggttgaagcc taccagcttg
     2341 ccgttttcgg cgattgccac gaagttcatg tcgttggcat gcagatcgat atcgttctgc
     2401 ggcgggatca ctaccgtggt tttgaactta cgcggcaggt aggtctgacc aaggatcggt
     2461 tcttcatcag tagtggcgac tttttcctgg tcgagccaga tctccgcata cgcgcgggta
     2521 cgaggcaaca gatgctcaga aatcttcttc gcccactcgt acgcttccgc gtgcagctgc
     2581 gactcgtaag ggttcgaggt gcagagtacg ttacggttca tgtcgttagc tgtcgccagc
     2641 gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt tgacgttctt tttcagaatg
     2701 ccgtggaact gaaacgtctg gcggttggtc aggcgaatgc tgccatagat ggtgttttca
     2761 ccggcaaatt tgtcgatcgc ctgccactgt ttagtggtaa tcaccccacc cggcagacga
     2821 cagcgaagca gcatcgcgtg gcgcggctcc agcttctgtt cagcacgttc ggcacggatg
     2881 tcgcggtcat cctgctgata catgccgtgg aagcggatca gcaggaagtt gtcgccctta
     2941 aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg tgccgcgcag gtagttgctt
     3001 tcaagcttca tgcgctcggc gtctgtcagt tttccttcga ccactaaagg ccctggatgt
     3061 ttttcgctca ttagtagaca tctcgctgat aacggcgctc tacgcgcagc tcacttaaaa
     3121 attcatccgc cgcttcggtg tccatgccac caaattcggc aatcacttcc agaagtgcct
     3181 gctcaacgtc tttcgccatg cgattagcgt cgccgcagac ataaatgtgg gcaccgtcat
     3241 tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt gtcttgtaca taaacttttt
     3301 ctttttggtc gcgcgaccag gcaagatcga tacgcgtcag cacgccctct ttgacgtagc
     3361 gctgccactc cacctggtag aggaaatctt ccgtaaagtg cgggttgcca aagaacagcc
     3421 agtttttacc cggcgcttcg tcggcggcgc gttgctgcat aaaggcacgg aacggcgcga
     3481 tgccggtacc ggggccaatc ataatcactg gcgtttgcgg gttagcgggc aggcggaaat
     3541 tgtcgttatg ttcgataaat acgcggactt cgccctcttc ttccacgcga tccgcgagga
     3601 agctggaggc accaccggca cgggcgcggc cttccacgtc gtaacgcacc acaccaacgg
     3661 tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc gatggaatac aggcgcgggg
     3721 tcagcgggcg cagcaggtta atcagcgctt cggcatccag ttgcgccgga gagaaacgca
     3781 ccatgtcgac aatcggcgtg gtcgcggcgt aatgctgtaa cttcgcttta tcgcccacca
     3841 gcggcagcag cgtttcgctg cgggtaagcg tggcatagtt ctcaacaatg ttggcggtgt
     3901 tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag aggcaacgtt ttgccctcga
     3961 cggtgacagg ttcatcgcct ttcagccaca gcagttcgac aagttctttc accagtgccg
     4021 ggtcgttctg ataccagaca cccagcgcgt cacccggctg gtaacgcagg cctgaatcgc
     4081 ctaagtcaat ttcgatatga cgaacgtctt tttcagagtt acgcccggta attttctggt
     4141 taaccgaaag gctcgccacc agcggcgcgt ctttgctgta cgggctggtg tggatttcat
     4201 ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac aggcgcacgc gatttaagcg
     4261 catcaaccac gcgggcgcgc cactcgctgg cagcagcctg gtattcaaca tcggcatcga
     4321 cacggtcgag caggcgttca ccacccagtt ccgccagctt gctgtcgaaa tctttcccgg
     4381 actggcagaa aaattcataa gagctatcgc cgaggctaaa cacggcaaac gcggtgtttt
     4441 ccagctttgg cgcttttttg gagaacagga acttatgcag cgcgacggct tcttccggcg
     4501 gttccccttc cccttgcgtt gacgtcacta cgatgagcag tttttcgctg gcgatttgtt
     4561 tgaatttata gtcgcccgcg ttcaccagct taacgttcag ttttgctgct aataaatcat
     4621 cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg cgaggcggag ataatagtta
     4681 tacccggcat ttctgcggct ggcgctggcg tcgctgcaag cgcagcaggc tgctgattga
     4741 gtacgcccca gaaatagcca gaaacccagg caagctgggt aggcgttaaa tcggtcgtgg
     4801 ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag caacgcggaa ggtgggacct
     4861 gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt ttctgcgccc tgtcagcgcc
     4921 cataaaacag aagagaagat aaggttaacg gggtgaacgg tgtggattaa agacgggata
     4981 gcgataacta ataaccaaat cgactaacct gttttagcaa tagtctttaa caacaaaata
     5041 gattaaccaa cccaatgaaa aacaaatgaa tttagccaat cattaagata aatcagcgat
     5101 tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag cctttccggt actctacggc
     5161 ggttttatcg tcctgtagag aaattatgat gtccaccacg ttatttaaag atttcacctt
     5221 cgaagccgct caccgcttac cacacgtccc ggaagggcat aaatgtggtc gcctgcacgg
     5281 gcattccttt atggtgcgac tggaaattac cggggaagtc gatccgcata cgggctggat
     5341 tatcgatttc gctgaactga aagcggcgtt taagccaact tatgagcgcc tcgatcacca
     5401 ttatctcaat gatattccag gtctggaaaa cccaaccagc gaagttttag caaaatggat
     5461 ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg gtgatggtaa aagaaacctg
     5521 caccgcaggg tgtatctatc gcggcgaatg ataagagtgt gtcggcggtc aatttccctt
     5581 aagtaacgct atgttagggt gttgtgttct ggatatctgg ggcatgacat ggaagacgac
     5641 tgcgacatta ttattattgg tgccggtatt gcaggcaccg cttgcgcgtt acgctgcgcg
     5701 cgagcgggtt tatccgtttt gttactggaa cgcgctgaaa tccccggcag caaaaatctt
     5761 tccggcgggc ggttatatac ccatgcactc gcggaactcc tcccgcaatt tcatctgacc
     5821 gcgcctcttg aacgacgcat cactcacgaa agcctttccc tgttaacgcc cgatggcgta
     5881 acgacgtttt ccagcttaca gcccggcggt gaatcctgga gtgtattacg tgcacggttc
     5941 gatccgtggc tggttgccga agccgaaaaa gaaggtgtcg aatgcatccc cggagcgacg
     6001 gtggatgcac tgtatgaaga aaacggcaga gtctgtggcg ttatttgtgg tgacgatatt
     6061 ctccgcgccc gttatgtggt gctggcagaa ggtgccaaca gcgtcctggc tgaacgtcac
     6121 gggttagtga ctcgtcctgc tggcgaagcg atggcgttgg ggatcaaaga agtgctgtcg
     6181 ctggaaacat ccgctattga agaacgtttt catctggaga ataacgaagg cgcagcgttg
     6241 ctgttcagcg gcggaatctg tgatgactta cccggcggcg catttcttta tactaatcaa
     6301 caaacgctct cgttagggat tgtttgcccg ctctcttccc ttacgcaaag tcgtgttccg
     6361 gcaagcgagc tgctgactcg ctttaaagcg catccggcag tgcgcccgct tatcaaaaac
     6421 acggaatcac tggagtatgg tgcgcatctg gagccagaag gtggcttgca cagtatgccg
     6481 gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg cgttgcgcag ttgcgtcaat
     6541 accggaattt ccgtgcgcgg catggatatg gcgctgactg gcgcacaggc ggcggcacaa
     6601 acgctaataa gcgcctgcca gcaccgcgag ccgcaaaatc tgtttgcgct ttatcatcac
     6661 aacgtagagc gcagcctgct atgggatgtt ctacaacgtt atcagcatgt tccggcgctt
     6721 ttgcaacgcc ctggctggta tcgggcgtgg cctgcgttaa tgcaggatat ttcccgcgat
     6781 ttatgggatc agggtgataa acctgttcca ccgctgcgcc agttattctg gcgtcattta
     6841 cgtcgtcatg gcctgtggca tctggcgggc gatgttatca ggagtctgcg atgtctgtag
     6901 cccataatct ctggcgcgtt gctgatgcgc cgcacattgt tccggctgac tccgttgagc
     6961 gtcagatggt acaacggttg attaacgcct gtccggcagg tcttttttcg ctcacaccgg
     7021 aaggtgactt acgcgttgat tatcacggct gcctggagtg tggcacctgc cgtttgctgt
     7081 gcgacgaatc cacactacaa cagtggcgct atccgccttc cggattcggc atcacctacc
     7141 gctttggata aataataagg ataatttatg cccctcttac acctactccg ccagaatccg
     7201 gtgattgctg ccgttaaaga taatgccagc ctgcaactgg ctatcgattc tgaatgccag
     7261 tttatttccg tgttgtacgg caatatctgc accatcagta atatcgttaa aaagattaag
     7321 aacgccggga aatatgcttt tattcatgtt gatttgctgg aaggcgcgtc aaataaagaa
     7381 gtcgttattc agtttttgaa actggtgacc gaggcggacg gcattatcag taccaaagcc
     7441 tcaatgttga aagcggcaag agcggaaggt tttttctgta ttcatcgcct gtttattgtt
     7501 gattcaattt cgtttcacaa cattgataag caagttgcgc aatcgaatcc ggattgtatt
     7561 gagatcctgc caggcggtat gcccaaagtg ctgggctggg tgacagagaa aatccgccaa
     7621 ccgctgattg ccggtgggct ggtgtgcgat gaagaagatg cgcgtaatgc gattaacgcg
     7681 ggtgtcgtgg cgctttccac cacgaatacc ggggtctgga cgttagcgaa aaaattactt
     7741 tgacgggata atcgtaacca attgaatttg gtttgatttg taggccgcac gccacatccg
     7801 acattcagcg cctgatgcga cgcttgacgc gtcttatcag gcctacaagt ccccaggcca
     7861 gaaccgtagg caggatcatc cggcaacacg ttattggcaa tctgcgtgaa tgtttgtcac
     7921 cagtgcctcc agcaccactt tccagtcatc aaccacgcca acatctgcct gtgaaaatac
     7981 cgcagcgctg gcgtcatgat taatcgccac cacaaacttg ctattgcgta cgcccgccat
     8041 taacgctgct gcgccagaag cgccaacaac aatacacacc tcaggtgcca gcagatgtcc
     8101 ggaaatacca atcacttttt cagcatcaac gccaccgttc atcactcgcg cccgactgta
     8161 gcccacttcg gctcccagct tttctgccag catagcgatt tcctggttat ctgcctctcc
     8221 gccctgcccc accaccagca cccgtctggc ctcagctaat gggtcgcgag taacattttt
     8281 cagatcctct gtgctaacca gccagtccgg aagcgcaccc gggacaatgt tcagttgttg
     8341 cattccagag ggcaacgtgg cgtttttcgc tgctcccgcc tgacgcgcca gggaaaggca
     8401 tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca tttccccagt gggatttgcg
     8461 cacgcttacg gttggaatat ctaacgaagt cacctggcag atgcttgcac catgtaaacg
     8521 ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc ggcggaaaga gaacaacgtc
     8581 cgcaggcgtt cgctgccact gttccaccaa cgcatccagc acctgttccg ccactacagg
     8641 ctgaggttca atctgccagt gcgccagggt acaaccgctg aaatcctgcg ccgccagcca
     8701 gcttgctatt gcggcattct cctgatttat tgtcacaatt gcgatattca tggctgcatc
     8761 ctttggcgta aatagtcctg ccatagcttt tgagcttttt ctgctaccgt ttgcccatca
     8821 atcagcgtcg ctccgcgtcg ttgctctgct cttgccagtt gcagacactg catcgctggc
     8881 atttcggcag caacagtttt acggataatt tctgctttcc ccgcagccat tcgctggcgc
     8941 attcccggta ctggcaaggc cacttcaccg cactgacgca cggcaatcac cgcaggcaag
     9001 cgaacccggc aacaacgcaa cccatgttca gtacgctgtt cgagggtgat aaacagcgcg
     9061 tcgagagtga aacgctccac ctgggtgaag cagggccagc ccagcatttc cgccagcaaa
     9121 aatggcgttt gcccattctg cccttcgctg ctttggcagc cggtaattat cagatccagc
     9181 ggattctgat gctgccattc ggcaatatgg cgagcgacaa attccggcgc aaagcgcaga
     9241 tctgccgccg tctccagcaa tacagcttcc tcaaacccaa gagccatgag atagcgcagc
     9301 cagtgcaatg cccgttcatc ccccatgctc aacgcggtta aagacatcgg cgtaccgttt
     9361 tttctctgcg ccaacagcag cgcggcagcg gcctgttcat cagcaccgag taaacttcgc
     9421 agtagcgaaa tatccggtcc gcttttaccc tgagccgccg cctgccactc tttttccgcc
     9481 agcattccgg catccggttc ggctttaaac gctaacagaa tgttcattcg cacctcctac
     9541 atttcccgcc gccaccagcg ggagggcttt agtttctggt gcccatagcc aggtcacaac
     9601 aaaatcaacc aacaacactg tcgccaggag caataaggtg acttgcattc cccactgcgc
     9661 cagcacccac ggcagcaggc cagtacttac cgccgcgcca agtcgactca tggcggtggc
     9721 aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt tccgcaggca aaatgcccac
     9781 cagattactg actgccgaaa tggtggtgct gaagagaaca aaaagcagca gcgttaatga
     9841 actgccggaa ggcaaacagg ccatcactac cagcgttgcc gccagcagca aaaaacttcc
     9901 cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact aatcccagca gcgcgcccac
     9961 aattaacaac gcattaagca tcaggctggc agtcagcgca tcttccagac caatagtctg
    10021 ggcgatagtt ggcagccagg tataaatcac aaaccatggg attacgaggc agacaaagaa
    10081 gacgctgtta aacgccgtgc gccgccagta acgcgaagag aacaaggttt tgatgtgttt
    10141 atgggtcgcc gttaccactt catcgcccag taaaacatgg ggaccaaaat agcgatgcac
    10201 gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc cagcgtggtg attctggcgt
    10261 tccccagcgt aataacgtaa tcaacaacgc gggcagagct gccgatgcca gtagccagcg
    10321 ccaggcctcc gggttttcgg aaataaagtg atgtccggca atacttgcca gcacatagcc
    10381 tacggtccac accacgctga atgcgcccag caaaataccg cgatggcggc gcggggaaaa
    10441 ttcagccagc aaggtgtgac ctactgaata atcgcctccc agaccaatgc caatcaaaat
    10501 gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat tgtaaaaacg aagcaagcgt
    10561 aatcagcaaa aagctgaagg tgaagatttt ttgccgacca atatggtcgg agatccaccc
    10621 aagaaccagg ctgccaagga acaaaccaag gagcgccgag ccgccgatca ttcccgccat
    10681 aaacggcgtc agttgcatgg cgggcgtaag ctgaataatg gcgtaaccaa tgacgccgag
    10741 aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca gcaatgcggc agtgaaaacg
    10801 gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc atctttaccc cttcattgtc
    10861 cggtaataag aacggcttct cgcctgagaa gccgaaaagg caacgccccg tcatcgcgga
    10921 acgctgcctg acacattatt tttcaatcgg atagatagta cccgtattca taatgccatt
    10981 aggatcgaac tgctttttca gcccttccag caacgcccac gcgctgccgt gttccagttt
    11041 gctccagtga acgcgatgtt taccgatacc gtggtggtgc accatcgaac cgccgaggcg
    11101 aatggtttct tcacagatga tcttgttgag cggattgtgg tacttgtcga tttcctcttc
    11161 cggcttacag tcaacgacgt tgtaatcgta gacgaagtac atgttggtgc cgttctgata
    11221 gctatgagag gaatgaccgc ccagcatggt gatgtcgtcg gcgtgcggga actcagtacg
    11281 aatacggtta ataacgcttt cgtagatttc gtggatgcag ctccagcagc cggacacttc
    11341 agtggtaaag cccatgttgc cggttttgag gatctgcaca cgttcggcag ccactttatc
    11401 cggtccccag ttcaggttgt taaaccaggt ttcgatcagc ttgctgtcca cgcgctggca
    11461 ttgcgggtag cgggcaacga tttccgcaat cccttcgccc gtcgccttcg caatgcgagg
    11521 gttaccttca gccataaaga tcagcacgca ttttccgtct gcaaaatggg tgaagtgttg
    11581 ggtgccatct tcagcgtcat acaaacgagc gatcgacgga cgataccctt ccaccatgac
    11641 ttcacgcagg atgttgaagt cggttttcat gtcttccagg atatagccgt agaagaggtt
    11701 gttttccggg gtgaatttaa agattttcac tgttacttca gtgatatagc aaagtgcacc
    11761 ttcgttaccg atgatgatgt ggcgaatgtc cgggccagcc gcgcggcgtg gcacgttttt
    11821 aatgcgtgtg acggtgccat cagccaatac tgcttccaga ccaacgacca tatcttcgat
    11881 tgcgccgtag agtgtggaga actgcccgat actgcgggtt gctaccaagc cgcccatctg
    11941 cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa cctttttcac gcaacgcatt
    12001 ttccagcacc tccagcggaa ccccacattg cgccgtcgcc tgcatattct caatatcaat
    12061 attaatgatt tgattcatgg cggagccgtc aagcaccacc gagttttcca caacggtttc
    12121 cagcccacct tcagtggcgg aagcgccggt acgcggcaca ccgttaattt tgtgcgcatt
    12181 cataaaattc agcacacggg atacttgctc tgtggaaccg agttttacga ccgctgccgg
    12241 aatcggcaaa gtataaatgc catgaatatc cggaaattta cgaaaacggt caatactgtt
    12301 cttctttaat actgtttcat cggtaattac gcgatctgca ccaacaattt cctttagttg
    12361 gtcgacaatc gctgcgcgag ataaagacat agtaattcct tcctgataat aaaaatggaa
    12421 taatcattaa taagagaaaa cagaaaccac cgttatggat tagcgcacta aataaccacc
    12481 attgaccact aataaatgcc cattgacata atttgaagcc ggacttgcga ggaatacagc
    12541 tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat caagaacgcg
    12601 ctgattggtt tctggattac tgcgtgtcgc cagcgtaata tctgttgcat aatagccagg
    12661 agcaatacca tttacctgaa tattatattg acctagttca tcacaataag ctttggtgaa
    12721 tccggcaaga gcatgtttag tggcagaata tgcaggtgac cattgtccac ctaagtaaga
    12781 gaacaatgaa cagatattaa taattttgcc gcttttctgc gggatcataa tttttgcagc
    12841 ttcatagctt aactcgaatg cggcggtcag gttcacatca atcatcggat cccagtcggc
    12901 acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttta ccagaatatc
    12961 aactgtaccg aaacgctcac agcaagcagc gataatcttc tgcggcgcgc cttctgcggt
    13021 gatatccacc tgcatgaagt ccacctcaac accctgtttt tcaatcattt cctttgtttc
    13081 gccgttatct ttcaatcatt tcctttgttt cgccgttatc tttgacgaaa ctaggaataa
    13141 agatatttgc gccagctttg gccaacgcca tggcaaatgc ctggcctaaa ccgctattcc
    13201 caccggtaac aattgcggtt ttacctttca gtgagaaaaa atccattgag aacgcattga
    13261 gagattcgat tgacatagtt agctccaatt tccgtaaggc aaaaaaaaga aaggtaactt
    13321 cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat aatttttagc
    13381 ataacggcat aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag
    13441 ttttaaaaag cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct
    13501 tattgcaatt actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct
    13561 cttttttttg tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg
    13621 ataaccctcg cgataattag ttttagcggc ggcgttagtt ttgacctggc ttatttacgt
    13681 tatatttttc aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta
    13741 ataatgagta cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc
    13801 gataaatttt cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt
    13861 ctgttaatgg caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg
    13921 ataacgacga ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt gctgggcgat
    13981 catagtgagc aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg
    14041 tcgctggcgg tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc
    14101 ctgaaaacag tcattatcat ctacagtgtg gtttacatct tgttggggat tctgtgctgg
    14161 ttttttgtta gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc
    14221 cagcttagcg acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg
    14281 atttttggtg tcttcacaat ctacgccatt ctgagttact ccaccaacta tctgaccgaa
    14341 atgtacggca tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa caaaatcttc
    14401 cgcgcgctgt gcggcccact tggaggcatt atcaccacct acagtaaagt gaaatcccct
    14461 acccgcgtga ttcaaatcct ttccatcatc ggtttgctgg cgttgacagc cctgctcgtc
    14521 acgaactcta acccccaatc ggtcgcgatg gggattggcc tgattttact gctgggattc
    14581 acctgttacg cctcacgcgg gctgtactgg gcctgccccg gcgaagcgag aacaccgtct
    14641 tacattatgg gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc
    14701 gtttacccga ttatcggcca ctggcaagac accctgcccg ctgcagaagc ctaccgcaat
    14761 atgtggctga tgggcatggc ggcgctcggc atggtgattg tctttacctt tttgctgttc
    14821 caaaaaattc gtactgctga tagcgccccc gcaatagcta gcagcaagta agccaggaaa
    14881 attacaggag aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga
    14941 gcacaaaagt ggtgatgtac gatctggaag gtaatgtggt ttgcgaaggt aaaggcttat
    15001 tacagccgat gcacacgcct gatgccgata ctgcagaaca tcctgacgac gatttatggg
    15061 catcattatg ttttgcaggt cacgatttga tgagccagtt tgccgggaat aaagaagata
    15121 ttgttggcat cggcctgggc tccatccgtt gctgccgtgc gttattgaaa gccgacggca
    15181 cgccagctgc gccgttgatt agctggcagg atgcacgcgt tacacgccct tacgaacata
    15241 cgaatcctga cgtggcgtat gtaacctctt tttcgggtta tctgacgcat cgcttaaccg
    15301 gcgaatttaa agataatatc gccaactatt ttggtcagtg gccggtggat tataagacct
    15361 gggcatggag cgaagatgcc gcagtcatgg agaagtttaa tatcccccgt catatgctgt
    15421 ttgatgtgca aatgccaggc actgttcttg ggcatatcac acctcaagcc gcgctggcga
    15481 cgcatttccc ggcaggactg ccggttgttt gtaccaccag tgataaaccg gtagaagctc
    15541 tgggagccgg attactggat gatgaaactg cggtaatttc tcttggcact tacatcgcat
    15601 tgatgatgaa cggcaaagcc ttgccgaaag atccggtggc gtactggccg attatgtctt
    15661 ctattccgca aacattgctg tatgaaggtt acggtattcg caaaggcatg tggacggtga
    15721 gctggctgcg cgacatgtta ggcgagtcgt taattcagga tgccagggcg caggatcttt
    15781 caccggaaga tttactcaac aagaaagcct ctagtgtgcc acctggctgt aatgggctga
    15841 tgacggtgct ggactggctg accaatccgt gggaaccgta caaacgcggg attatgatcg
    15901 gctttgattc cagcatggat tacgcatgga tatatcgttc gatattggaa agcgtggcgc
    15961 tgacgctgaa gaacaattac gacaatatgt gtaatgaaat gaatcacttt gcgaaacatg
    16021 taatcattac tggcggcggt tcgaacagga tctgtttatg cagatttttg ccgacgtgtt
    16081 caaccttccg gcacgccgta acgccattaa cggttgtgca agcctggggg cagcgattaa
    16141 tacaggggta ggtctggggc tatacccgga ttacgcaacg gctgtcgata aaatggttcg
    16201 cgtgaaagat atctttatac cgattgagag caatgccaaa cgctacgacg cgatgaataa
    16261 aggcattttc aaagacctaa ccaaacatac tgatgtgatc ctgaaaaaat cgtatgaagt
    16321 gatgcatggg gaattgggga attggggaat gcggattcga tccagagctg gtcgaatgcg
    16381 tgaataaggt aggccgggcg gaaaactgcc cggcctgaac atacctgaat ggttatcccc
    16441 gctggcgcgg ggaacataag ttcgacaact taaaaaatga taacaagtcc ggtttatccc
    16501 cgctgatgca gggaacataa taaaaacttt aactagttcg gtttatccct gtttttacaa
    16561 ggaa
//
LOCUS       DAZOXR010000083        16555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZOXR010000083 DAZOXR010000000
VERSION     DAZOXR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.085"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHUNN_002371"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHUNN_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR0576730.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACHUNN_002372"
     CDS             complement(1899..2636)
                     /locus_tag="ACHUNN_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576731.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHUNN_002373"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHUNN_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR0576732.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACHUNN_002374"
     CDS             complement(3584..4093)
                     /gene="traS"
                     /locus_tag="ACHUNN_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR0576733.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACHUNN_002375"
     CDS             complement(4090..6915)
                     /gene="traG"
                     /locus_tag="ACHUNN_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR0576734.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACHUNN_002376"
     CDS             complement(6912..8285)
                     /gene="traH"
                     /locus_tag="ACHUNN_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR0576735.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACHUNN_002377"
     CDS             complement(8272..8664)
                     /gene="trbF"
                     /locus_tag="ACHUNN_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR0576736.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACHUNN_002378"
     CDS             complement(8645..8992)
                     /gene="trbJ"
                     /locus_tag="ACHUNN_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR0576737.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACHUNN_002379"
     CDS             complement(8922..9467)
                     /gene="trbB"
                     /locus_tag="ACHUNN_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR0576738.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACHUNN_002380"
     CDS             complement(9454..9738)
                     /gene="traQ"
                     /locus_tag="ACHUNN_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR0576739.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9819..10154
                     /locus_tag="ACHUNN_002381"
     CDS             9819..10154
                     /locus_tag="ACHUNN_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR0576740.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACHUNN_002382"
     CDS             complement(10135..10476)
                     /gene="trbA"
                     /locus_tag="ACHUNN_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR0576741.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACHUNN_002383"
     CDS             complement(10490..11233)
                     /gene="traF"
                     /locus_tag="ACHUNN_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR0576742.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11226..11483)
                     /locus_tag="ACHUNN_002384"
     CDS             complement(11226..11483)
                     /locus_tag="ACHUNN_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR0576743.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACHUNN_002385"
     CDS             complement(11510..13414)
                     /gene="traN"
                     /locus_tag="ACHUNN_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR0576744.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(13650..14030)
                     /locus_tag="ACHUNN_002386"
     CDS             complement(13650..14030)
                     /locus_tag="ACHUNN_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576745.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACHUNN_002387"
     CDS             complement(14027..14665)
                     /gene="trbC"
                     /locus_tag="ACHUNN_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR0576746.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14674..14979)
                     /locus_tag="ACHUNN_002388"
     CDS             complement(14674..14979)
                     /locus_tag="ACHUNN_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576747.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACHUNN_002389"
     CDS             complement(15009..16001)
                     /gene="traU"
                     /locus_tag="ACHUNN_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR0576748.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15998..>16555)
                     /gene="traW"
                     /locus_tag="ACHUNN_002390"
     CDS             complement(15998..>16555)
                     /gene="traW"
                     /locus_tag="ACHUNN_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR0576749.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRVFARQGEVMNPLQYVPFNQTLYFI
                     NGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQNGVLCQRLGIDQV
                     PARVSAVPGDRFLKVEFIPAEEGRK"
BASE COUNT         4156 a         4443 c         3779 g         4177 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaggcaac ctccggttcg aagaggctgg ccatctggcg
       61 accttctgct tcccttgcag caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgtc tgatatttca gtgagagttt
      181 tactgccgga tacggtccgg gcagagtgac ataacaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctggcgctc catctcctta ccggtcgata ccccgtcacg
      301 cacggggtca gcaccgtagg aatactgcag actggctttc aggtgctctt tctcaccaat
      361 ttcaccggca gcaaactctg caatctgatg gctcggggag cggaaaaagg cacgggtatt
      421 cagcacatca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagcctcc gaacttacgg gcttccggca ggatctccac
      541 caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggaaaccac
      661 cggcttcagg gaggcatgag tgtcggcatt tgacgagata aacagccagc cgtttttctg
      721 atcttcccgg acaccccgca tccagtcacg tatggtgaag ggttcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgatc cctgccagaa
     1021 cgggtcctct ttcgttccca tcgggatcag cgtgttcgcg acattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcaggat
     1141 cttatcgatg gagggatcat agtaactttt aacaaactca cacgaacggt cataaatcac
     1201 caccatgtcg ccacgtttac gggcatagtt ggcaagacgt cgaataacct ctgacttacc
     1261 cgtcgacact gttccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 atcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     1501 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttgcaggc
     2581 ttctcagtaa attgattatt ctcaccaata tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccca caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaacagc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctgcatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggtttaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcatttttt ttgttttcat ccagaatatc cttttaacta acagactctt
     3601 ttttgaacag acctattact gagactattg cagagagatt ataacgattt atatctatag
     3661 tataaataaa ggaaataatt acaaacagag ttattgataa aaacccaaat aagaacgtat
     3721 gagcaaagga aactattgaa aaaacaatca caaacgacaa gatataagtt ttgttcttat
     3781 catacataaa agatataact ttcgactcat ctctgaattt tttagggatg gcaagataca
     3841 acatcattcc attagtaatg gcaacaaaaa ggataaaacc cattacgcca ctaaaacata
     3901 tatcaaatcc agcatctcgg atatcaactt tatctgccag caaacgaaga aatgtcggcc
     3961 ataataaaac ccataaaaac acatacaccc ctggttttat gacttgtaat aatggcggaa
     4021 tattaactcc ctctacagag agtacatcct ttagtttctg tgtctccgtt tttatatcat
     4081 agtgagtgat cataatggcc ccttcagtct atccttttta tcatcaacca tttttttcac
     4141 agcctcttct gatggctcag gtattatctt ttgactttct cgctcttcat tatatttatt
     4201 attctgagat aatgcctctg ttttatgatg attttgcagt tcagaatact gccctcttac
     4261 aatattttcc tcgccacgga tactgttctg agtatctccg atattgccac gatattctgt
     4321 gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct gcgttctttg
     4381 ctcaataatg gcctgatgtc cctgatggtc agcaataata tcctgactgc caccacccga
     4441 aggtacgctc tccattcctt taccaatatc gccacgggat tcccgccagg cattatcaac
     4501 accaggctgt acctgttcct gcacaaaaaa ccaggccatc gcccgacgac gttctgcaat
     4561 ctccggcgaa ctggtatttg tcagaatagc ttctgcatcc tgcggtgcgc gtttcatcac
     4621 atactgtgca aactgttgcg acaggtcttc actcatctgc ccgctcatgc tttcagtacg
     4681 ggaggccatt tccgcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
     4741 ctgtttcgct gagttcaggg ctgcagacaa ctggtccacc cgggagtcag cattattgtc
     4801 agtatggctg ccggactcac tgaccttgcg actggtaaag taatcgctgg cttctttaaa
     4861 gtccttactg gctttagcgt caatatcatg gcgagcatca tgggtagctc tgctgccact
     4921 acttgcagaa tgtgaatcat catccgttcg atcaagactt cccttaaacc cgcctctgac
     4981 accaacccca ccaccaacac caagaattcc aggtttaatc ccccattcag catgagcatc
     5041 tgcatatccg ccatacgaga acttagaacc tgttgatgcc agttcctgag tcgcctgctc
     5101 attactgata ttatgcgcct tcgcatagct ttccactgca ctgcgcatac ggctggccat
     5161 catggagtcc tgtgcactca tggtgctgtc agcgccaccg gtgacagaat cactgctccc
     5221 ccggttagaa ccaaactgac tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
     5281 atgcagggca ctttctgctc tgttcgacgc ctcccgggcc atttcctgtt gcgccgctgc
     5341 aatctgacgc gttgcattga taccgaccgg taaacgggac atcgctccgc ttgcatccat
     5401 caccatatta ccgtcacggg tctgtgtggc agttgcgcca ctgccggtct ggtacatcat
     5461 ctgaccaaac gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tttccgtctg
     5521 catgttgccg taggagtaat taccgtcaac cacactgcct gctgcactgg ccgtcggact
     5581 gatagaggaa gaggcgaagt ggctgtacac gctggaaaaa cctgcgccaa ggccttttac
     5641 catcatccat gacaacgggg ggatcatggc tgaaagatac ccggcagtag aggccaggtc
     5701 agagtatttc agctgtatct gagagagttc agacagcacg accggcgcac cattcatctt
     5761 cgcatagaat gtcatggcac tgttcagaat ggcatacaac aacggccagg tctgcagcca
     5821 catcagggca aacacatagc ctttcaggac ggacagtgtc agtttgttga agacagctgc
     5881 cagtaccaga agcgggaaaa taccaatggc aattcccgtc aggatagtct gtaccattgg
     5941 caggtttcgc attgtcacat gaccgataga gacatgcgcc agacgttgct tctccatcga
     6001 tgacgtggtg gccagattca ccaggctggc ggtgtcacca ttacgggcag cattactggt
     6061 gataccctct ttcagggcat ttattgtgac gttctggcgc ataatctggc tggcagactg
     6121 actggagccg tagaaataac tgtaactgtc actgaccagt tgtctgaata acaggtccgg
     6181 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctcccgtctt
     6241 tgtatcgaga ttcagcctgt ctttcagcgt gaccgacgca tccttacagg ttatgaaatt
     6301 attattgttg tcataaacgc cccgtagcgg gctgggtcgg gaaaaaatca gcgtgtaggg
     6361 gtcagcggag gccatcagat cttccagcgt gtatttatga ttcaggtaaa tatcacccag
     6421 cacgcagttc tggacgtagt cctggaacag attgatgatt tccggattcc gggacaggaa
     6481 atcggtgctt ttcacaatca gattcgcccc gaacagcatg cctgtcttgc tgtaggtgac
     6541 gctgtccggt tgggtgaata tcatctcgta gctgaccacc atcgcatgcc cgatacgggt
     6601 cgtaagagaa agtggcatcg ccagaccgac cggaacatta tccacccgat ggactttgac
     6661 caggtcactg ttatcaataa tctgcaccga tgtgcggaca ttaaccagca ggctgataag
     6721 cacaaacacg gccacccagc ccagcaaatc catcacgtta tgccgctgta cccacataaa
     6781 agccacagca acaacagaca gggtgagcgc gattttttca attgaatccc aggtccgggt
     6841 actcataaat gcagcaatgg cgttcaggtt attccgcaac cactcaccac cggcaatcac
     6901 ataaacttca ttcacagtga attccctccg aagtgatagt tgttctggta acggctgagc
     6961 atacgggcgg aaagctgctg acgcatgtag ttcatctgac gttcgacaac cagcagcgca
     7021 tcctgctgga cctgtacctg actctggaaa atggcaatct gttgctgtgc ctgattcaga
     7081 ttctccagaa cattgtccat cacagcctgc ggatagttcc cggtggcaat catggcccgg
     7141 gcctgctgaa gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc
     7201 agctggtaaa cgacgctgtt ggacacaccg agcatctgcg ggtcaatcag atatttaaaa
     7261 acgggaatgg tggtgctgga aataaacccc tgttcccggg cgtcaagcgg agtgtcactc
     7321 actgctttat tctgaatact ggtgagcagt tttgtaatct gtgacttcag ggctttatcc
     7381 cgggcgatgg tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag
     7441 tggtaaattt ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta
     7501 cgcgccggca ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc
     7561 atgataaatt ctttcaactc tttgttgccg tcaaacattc tgtttttttt cagcgcctcc
     7621 catatgatat tctggttttt cagcacccgc tccttgtcct tgtcgctggc tttgtcacga
     7681 acagatgagg actgccctcc gaccgtgcac ccctgacgag atgcggccca gtcggcgaag
     7741 atattgcttt cactgccaat gtcctgacaa actttctgct gagattcctg cgtctgaggg
     7801 aacagccccc ccacaagccc ctgtgccacc tgacaggaac tgaggttcat actgttaatg
     7861 tcacttgcca ttttctgcag gaagtctttt gcggttttta tttccggcac cgttgtctgc
     7921 agggcaaggt caaaaaagta accggcagca ttgctcataa tctgcttaac aaaacgctgc
     7981 aactgatcgc cattaataaa actgaacgag ccgagatagg cgtcgatgcc gccacatccg
     8041 gcattgatat ccggcagcgt catggaaatc agctggatat ttttgacctg ggtgcgggca
     8101 tacagggagc caccataggc atacccggcg gcctgcccct gccagacgcc cggctgtgtg
     8161 gtattggagg cgaagcccag cttgttaaag aactgattca tatcgctgtt cacatccgct
     8221 gacgctgcgg gtgtgaccgt cagtaaagcg gcacaaagaa ccagaagagg tttaatgcga
     8281 agcattattg ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa
     8341 tatccggaca cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa
     8401 accagctgca agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata
     8461 acgaaaatta tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc
     8521 atgcataacg ttccttacaa tcaatccggc agcaaagaaa accacaaaaa gccagaagta
     8581 tttttttgca ttatcacaac cacgttcaat ctccctttca atttgtttga ttcccggttc
     8641 tgtatcattt attttttctt tcatcataag ttccctctga tatttctgat gattcatgca
     8701 gacaggtcat gcggctgtaa tatatttcag acaccgaaat ccatcccaca attgccccca
     8761 caaaaaacac aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc
     8821 tggcgggata aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg
     8881 tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc acctttttcc
     8941 tctccgtata tctgcagaac ggtatccatc cgcgccataa aactggcggc atccgttgcc
     9001 ccctgcaaaa gcggtaatgc ctcaagtgtg ttgacgttga ccaggaaggt ggtcggtgtg
     9061 gccaccggga tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc
     9121 ggaaaggcag tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc
     9181 tgtgccagct gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata
     9241 aacagcacca ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag
     9301 cggggagccg gcttttctgt tctggctgat gtgtctgctg ccggttgtgc aggctgcacg
     9361 gccattccct gcggattcca gagtcgttca atttcatccc gggtggaggc ctgcacacct
     9421 gtggccatca tcaacaaaat gaccggcagt aatttagtga gagacatgtc cgccctcctg
     9481 ctgcccttcc atcatggcct gttgcctggc ttccagtgcc tcacgctctt ccaggctgac
     9541 acgtgcaatc catgcatcca gcacctggta tgcgccgaac agcacaagga tggccgcaat
     9601 caactccgcc agaaaaaaag ccatccaggg tttgctgtaa acaagacggg cgacgatatg
     9661 aaaccagaca cccagggaaa atacccacat tcccgtaata tcaagccggg gtaaagagaa
     9721 tctgcgttta cttatcatgt taaactccgg ttattattta catgagagta ttcatgtctg
     9781 taatattcgc gtttgtgtga tattaatcag aggtacaaat gaaaaagtta tcttttaaag
     9841 aaaaactgga aatagtgaga aacatcataa gagagtcatt atcaggtaat gcggcttttg
     9901 ctttactggt gtattcatta ttgcactccc tccccattaa aacctttgct gatttatttg
     9961 tgacgatact gttatttata gcgattatcg ctgttgtgat atggctattt ctaatcatct
    10021 atatttattt ttgtgagtta ttcagaagtc actggatagc agtctggttt atcctctggt
    10081 ctgtggtaat caacctcact attggatttg gcatttatga caaattagca ccagtcattt
    10141 cactaatgaa ataataaaaa accacatcac tccagaccat atggctgaca acataataca
    10201 aacaaacatc cttctgaaac tgtcaaactt cgtgataacc ggtatggtac tgactgccat
    10261 tgaaacaacc ggtacggcac actgattcat aaccgcacca caaagaaaca agaaattatc
    10321 tggcagcata catacactta tgacatttac tacaaagaag acaaacgagc tcagcagata
    10381 aactccttta ttatttggtc ctgtaaagaa ataaccaaca acccccacga taaacaggct
    10441 ggcacctgta aaaaaacaca aatcccctgt atgcatatat aacctttggt taaaaattcg
    10501 gtttaaaatc ttcagaaacg ttcaggaact gttttgccag gtcgtcctgt gaaataaagc
    10561 cgtatgataa cgggcgaaca ctgccctgtt tcgggtcaac cagcatcatg gccgggaaat
    10621 atttcacacc gaggcgctgc gcctgcccct ggtcagtccg ggaatccggc aacagcggat
    10681 taatcacgcc atccacggaa acgggaataa cactcagacc atacgtatcc cggaagccat
    10741 taatgacctg cgccagttgc ccgtcgatgg ggtcctgccc ccggtaaaaa aacatgatgc
    10801 cgtagtgttc agccagtttc gcaatggcct gtcgctgctg cgcctggtct gccgccagct
    10861 ggttccgtac cgtgccgttg taatggctgt actgcaggtt atagtccagt tcgggatgtg
    10921 ccagcatggc ctttttagca ctcatggtga aaagcccggc ctgctgagtc cagtaattct
    10981 gcagccggaa atatttaaca aaattatcca cgccgggata cagaatggct tcgtacagcg
    11041 cccgctttgt tgccgtctgc agcgcggcca gtttctgcat aatatccggc tcctgacggg
    11101 gggctgcagg tacaggttta ttttcttttt ccttcggatt tattttctcg ttataccact
    11161 gccagcctgc atctttccct gacgccggaa aaataaagca gcagagtaac agtggcagta
    11221 atgctttatt catcttcatt cctttcatca ttttttcttt catcatcttt ctgcaggcca
    11281 gtcagggcag aaaaacaaaa atacagaata aacaggagca caccggagta aaggataacg
    11341 aaagcagcag caagtatccc gtctgccgta gtttctttta ccgtatccca cgaccagata
    11401 attaccggag aaatgacaat cgctgtaatc gaaagccgga taagaaaatc aacaagacga
    11461 ttagaagtga atataacttt catcatttcc cccggttatt ttccatacct tactgtcctg
    11521 cctgtttcag ctggtcagca atctgctctt tcactttctg cgtcagaacg ccactgtccg
    11581 ggattttctg gttattcatc aggtcttcgt agaagttagt gaaatccagt ctgtcgaact
    11641 gaattttctg cagctcatca accgtaatcc cccgacagtc aggatgcttc gcactgccaa
    11701 acccgatacg caactgcccg ttacgcccct gctgctggac tatctgcgcc agcttcgaat
    11761 caaactggca gtagctgcgc tttttctgca gacagacacc cagcactttc cgggagcaga
    11821 actcgcccac actcaccgtc agtttgttgt cctttgcctt tcccagcgcc ttttcttcac
    11881 tgttgcactt cgccagcccc acatcctgac cccagccgcc atccttacag cagttactga
    11941 agccggctgc ggccttacgg cagaatttcg cctcacccgt gaaagccctg acatccacac
    12001 cattcagggc ggcaacatcc ttacctgcgg cggcaagcgc agccagctgt gatactgctt
    12061 caccaaaatc actgtttttt ccgctctgtg ccttatcgca ttcaccgtca aggcagaaga
    12121 catcaccacc acagaccatc acttttcctg atgttctgga ctcgcaggag tacgtggcgt
    12181 actcatgcag acaggttccc tcatcggaat aaaacgcgca ctgacgtgag gccagcgtac
    12241 aggccggatt atcgacatat ttctgacagg tgccgttatc tgcggactga gtgacatatt
    12301 tatcccggta tgcccagcat gcctgtgtga tgttccaggg tttaccttcc atcactccgg
    12361 ttttcgtacc acccggctct gaacactcag tgcctgtcag cacgccatcc gctttattaa
    12421 acggacaact ctccacccac tccacccggg gaacccattc tttatcacgg accttcatcc
    12481 tgagtttcag cgtaaaggtg gaagcaccac tgacaagcga ttcatagact ttcctgtcac
    12541 cattcccgtg cgggaggcaa ttaccgttag cagtacagcc actaccggtc agaacctgcc
    12601 cctgtgtcac ctgcaaacct gtcgccccgg acagggggta agtatccgac tgcccgtttg
    12661 ccaggccgac attaaaggtt gtattcatga aggtataacg ggagttcagg aagaaaaaag
    12721 cggcgtatac agacaatgat gcactttcca ctgtaccggt taccggagcc gtgacactga
    12781 acaccagttt cagcccgttc atactgaacc ggaactgtga gtgcggtatc gtcacttcac
    12841 gatattcatc cgtgtatttc cagtccccgg tgatactggc agtccgtgtg cagtactcct
    12901 ccaccatcgt gtcccgctca caggtatagt ttgtgaattc actgcggttt atctcctgcg
    12961 cactgcactg ctgtccggta tttcccacaa tgctgtcagc cctgttcacc acatccctgc
    13021 cggtctgaat gaacggtgca tccggtgaca gaatgtcttt cggcttgttc atgaaggact
    13081 ctgtgatggt ttttccggtt tcaccggtcg cccattccgt ggtgccgtca tttttcaggc
    13141 cgccatcccc gccggcagtc acgccaccgt aatattttgt ctcgtccgga ttcgcgttat
    13201 agccggggat actctcctgt ggcttaaagc cctgaatact gctgcttccc tgccctttga
    13261 tctggtgagc aaaatcagaa ccggcccggt aatcactgtt gctgtcagca aacacagaaa
    13321 agctcagaaa aaaaacaatt gcacaaagat tgcacggctt tacggaataa ttaaaattca
    13381 gagatctgaa aaaggtattt atcttttctt tcataacctg aaccatactg gagaatgaag
    13441 tgatgtcatc tcaacttcag aactgtgttg attgtggtaa agcattaagt cgaacagcca
    13501 caacgtgcgg gaattgcagc tcaacggacc cttttggaaa aaaacggcgc agcgatcgct
    13561 tttaccagtg gtttgtaggg ctgtttatta cggctattat tattgctggc ggattatggt
    13621 acatggggct tcttgacgta ccgggattac tactcagata acgacttcag taacgatatc
    13681 agtgttctga aatcggcaca taacgagtga cgacaatacc ggttatacca gtaatgaggg
    13741 gtgtcgggct gaagcctgaa acagagctga aacttactgt taaaagcccc cttcaccgac
    13801 catccccatg ccagacgtgg tattgctgat tcagcatcct gccgcagtcg ctgctcaaga
    13861 agggcagcca tttttaccgg acaggcaaag gcaatataag gaggcagctt tgtaacaggg
    13921 aaaccatgtc cctgacagga agcatatgtg cggaagccgt gaatgttcat cgcggccacc
    13981 agggcacgga tctccggatc aatctgataa tacccggcag gtaaagtcat ttttgtttat
    14041 ctcccgggtt attcagcaac gcccgagcca cctgccggca gtcccccgtg gcggccactt
    14101 tctccagcgc ctggccgacc cgcaggttac ctcggataat gtcgtatccc tgactgcaga
    14161 acaccaccag tgccggtaca ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct
    14221 gaacaccatc agtcgcgccg tctttcacca gggacagcac ggcttcagcg gtggtcttca
    14281 ggtcattatt caccatgccc cgcagtgtgg cgggtatacc gtagtgccgg gtttcaccca
    14341 gcatccgttt cagcccctct tcgggaatgg aaaacgacac aaaatacagg gcaccctgcc
    14401 ggggtttccc gtcctgcgaa gcctgctgtt tacgaaccag ctcatccaga aaatggttat
    14461 ctgaacgctg aagggggttt tccagcacct gtttctccgc ccaggctttc agctgatggt
    14521 caggtttctc tcgcagttgt ctgcttaaat tttcctgctg cttcaggaac tggcggtttt
    14581 caggtgtgtt cacattttct gaagcatgaa cagcaagatg catccctgct gtcagagcaa
    14641 aaagtgcaaa tattttccgg ttcatgatat ttctcacttt ctgctattta caggcatgaa
    14701 ctcatcagaa aaaagataat tgagaaaaac agccataaac gcacatgtaa atgtcacctt
    14761 cagatataat ctgacaagtg tcacttgttc tgatatatct gccttcttcc attcatgaat
    14821 aatttcagcg agaaaactgt caggtgcaaa ataaaataat ataacgggca ccattacaag
    14881 accaaaagga aataatttta atgttctgct tatcagcctg agcagcacag cacttatttt
    14941 cctgaggtgc tgaatatatt tactgtccat tgttgccatt ttcatttcct cttattcatt
    15001 aaaatgattt acaggaatac acagttacgt ttacgccaca tcaggtagcc gaagtttttc
    15061 tttgtgttgg gcggattttt tcccgtctcc cagcgcatca cactgcgccc gaacggatgg
    15121 cactgcccgc tgtccggata catattcacc atctgataac gccagcgttc tttgggcagg
    15181 attggggacg gatattcatt acagacggcg ttatttttcc cgatggtttc cataatcatg
    15241 ccctgacggt gtagtttgaa cgccatgcgt tcactgacca gcagggagga ctgcaacgga
    15301 ctggactcat tactcaccca gccattgaac gggtacatac ttccctgcga accggcacac
    15361 cagaacagaa catcgagagg catattaaag gcgctggcaa tggcatctgc tgcacaggcc
    15421 ccctgtgcta tcggattggc aaagatgaca gcttccggat tgagaatggt ggtcaggctg
    15481 ctgtccgtcc aggtggggtc aatttcagaa agataagcga tatccaggtc accaccttcc
    15541 agacagccca gcgatgtgat gatgttcagc cagtacgtca gcgggtattt gtaccagtga
    15601 acgtgataga acgccccgtt cacctgcttc tcatcctttt tcgccgttcc ctgtgccgtt
    15661 ttaccaaaag ccggcaggct gaagcccagg ttaaccatgc atcccggtga ccgggtgacg
    15721 tccgtcagcg ccatcggctc ccagtaacca atggccagcc cgatacgcct gaacagcggc
    15781 ggcggtgccg gacaaatctg aatgggcatc gacgggttcg ccgtgtcggg gaccttaccc
    15841 tgactgactt tgacactgcc cagcgagagc gggaaaatac agctccagca gatatccgtg
    15901 atcgggttta caaaacgccc ctcacaggca gaatccgcag acgctgcagg gacatgaccg
    15961 gcgagaagga ataacatcag cagccacagg cttcgcttca ttttctgccc tcctctgccg
    16021 gaataaattc caccttcagg aagcgatcgc cgggaacggc gctcacccgt gccgggacct
    16081 gatcaatgcc gaggcgctgg cagagcacac cgttctggtc aaagtaaaca cggctgtcca
    16141 gagacttctg catctccggg atactgccct gcacgaggat aattttgctc tccagtgtgg
    16201 gcggcgtctg gcgtttcatc caggccacct gcgccggatc atcgccattg atgaaataca
    16261 gcgtctggtt aaaaggcaca tactgcagcg gattcatcac ttcaccctga cgggcaaaca
    16321 cccgcccttc gttatcccgg atatccgcag ccagtctgac ggacgggtca aacaaccggc
    16381 tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg gggccgcaga ctgttgcgga
    16441 tcacccgctc cttaaaggca tccattttcc ggcccatctc accggactgc tccagtgccg
    16501 tcaggcgctg catgatcacc gtcagcatgt ccggctcctt taccggccac agatc
//
LOCUS       DAZOXR010000084        16514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZOXR010000084 DAZOXR010000000
VERSION     DAZOXR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.086"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUNN_002391"
     CDS             <1..188
                     /locus_tag="ACHUNN_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576750.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHUNN_002392"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHUNN_002392"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0576751.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUNN_002393"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUNN_002393"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0576752.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUNN_002394"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUNN_002394"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0576753.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUNN_002395"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUNN_002395"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0576754.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUNN_002396"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUNN_002396"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0576755.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUNN_002397"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUNN_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0576756.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHUNN_002398"
     CDS             7142..7351
                     /locus_tag="ACHUNN_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0576757.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUNN_002399"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUNN_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0576758.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8226..8480
                     /gene="yjbL"
                     /locus_tag="ACHUNN_002400"
     CDS             8226..8480
                     /gene="yjbL"
                     /locus_tag="ACHUNN_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0576759.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8504..9211
                     /locus_tag="ACHUNN_002401"
     CDS             8504..9211
                     /locus_tag="ACHUNN_002401"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0576760.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9574..10611
                     /gene="dusA"
                     /locus_tag="ACHUNN_002402"
     CDS             9574..10611
                     /gene="dusA"
                     /locus_tag="ACHUNN_002402"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0576761.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10745..10987
                     /gene="pspG"
                     /locus_tag="ACHUNN_002403"
     CDS             10745..10987
                     /gene="pspG"
                     /locus_tag="ACHUNN_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0576762.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11153..12136)
                     /locus_tag="ACHUNN_002404"
     CDS             complement(11153..12136)
                     /locus_tag="ACHUNN_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0576763.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12219..13634
                     /gene="dnaB"
                     /locus_tag="ACHUNN_002405"
     CDS             12219..13634
                     /gene="dnaB"
                     /locus_tag="ACHUNN_002405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0576764.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13687..14766
                     /gene="alr"
                     /locus_tag="ACHUNN_002406"
     CDS             13687..14766
                     /gene="alr"
                     /locus_tag="ACHUNN_002406"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0576765.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15019..16212
                     /gene="tyrB"
                     /locus_tag="ACHUNN_002407"
     CDS             15019..16212
                     /gene="tyrB"
                     /locus_tag="ACHUNN_002407"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0576766.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3940 a         3930 c         4355 g         4289 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcactaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacggt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc aagttaactc
     8101 gctatttatt taactgggtt taaacgcata cctgtagttc agaaattatg agcgattttt
     8161 gatagtgacc agatggcaaa agaattataa ggaggacacg gtacgacaaa aaaggtagat
     8221 gaatcatgtt gaagataata cccggtgcaa ctggatactt taataaaaca ctcaattcaa
     8281 atcaattcga taatgaagat gcaatcaaag ataaattaga taatagaggt tcaatcaaag
     8341 gtaaattaaa taatatatat ggtaaatcta ttgattatgc agcgctgcgt catcgggata
     8401 taattattgc aaaaatagat ttgtttattc aacgaatcac gcataattta tggcatgcac
     8461 gtaaaaaaat gtgtttttaa ttgagcaaat aaatgatctc aaaatgtggg tcaataagta
     8521 tattgacgat tgcactgatg aggatttaaa cgatcgtgac tttattgcat cagttgttga
     8581 ccgggctatt tttcatttcg cgattaatag tatatgtaat cctggggata ataaagatgc
     8641 gactcccatt gaacaatgta cttttgatgt agaaactaag aatggccttc cctccacggt
     8701 tcagctattt tatgaggaat ctaaggataa tgaaccttta gcgaatatac attttcaagc
     8761 aataggttct ggttttttaa cgtttgttaa tgcctgccag gaacatgatg acaacagctt
     8821 aaaattattt gcttcactgt taatttcact ttcatattct agtgcctacg cagatttatc
     8881 agaaacagtg tatattaatg aaaataatga gagctacctg aaagctcagt ttgaaaaatt
     8941 atctcaacgt gatatgaaga agtacctggg agagatgaag cgtctggctg atgggggaga
     9001 aatgaatttt gatggctatc tggataagat gtcacatctg gtgaatgaag gaacgctcga
     9061 tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa ttaattagct tcgcgaagtc
     9121 gtttgaccca acctcaaagg aagagattaa aatacttaca gacacttcta aattaattta
     9181 tgatttgttc ggggttaaat cggagaaata atatgtgaag ttcttcgata gtatggaagg
     9241 cattatataa aaggacccaa tatttattgg gttctttttt ctctatcaat gctattagca
     9301 gggagatata tcaccagagt ttaatgtgtg attttttatt tatcgtcgaa cctggattgt
     9361 ttatcattgg ccttaacaaa gttaacggct aataagatta tttccatcac ttcgtgagag
     9421 cttcatgcct tgagaggatc tcaattttct tttgcaatga gacaggcgct tcctgttgtt
     9481 atggtatagt accccgctat tgagcctcct gaatagtgat gctgaataac ataaccccat
     9541 gatatatcga taaaataatc tctacatttg aaaatgcacg gtaattctga aatgcaaaaa
     9601 atcaaccaaa ccagcgcaat gcctgaaaaa actgacgttc actggagtgg tcggtttagc
     9661 gttgcaccaa tgctcgactg gacggacaga cattgccgct atttcttgcg tctgctttcc
     9721 cgcaatacgt tgctgtatac cgaaatggtg accacagggg cgattattca cggtaaaggt
     9781 gattacctgg cgtacagtga agaagaacat ccggtagcgt tgcaactcgg cggtagcgat
     9841 ccggcggcgc tggcacagtg tgcgaagctg gcagaagcgc gtggatatga tgagatcaac
     9901 ctgaatgtcg gctgcccgtc tgaccgggtg cagaacggca tgtttggtgc gtgtctgatg
     9961 ggtaatgcgc agctggttgc cgactgcgtg aaagcgatgc gcgatgtggt gtcgattcca
    10021 gtgacggtga aaacgcgtat tggcatcgat gaccaggaca gctatgaatt tctctgcgat
    10081 ttcatcaaca ccgtttccgg caaaggcgag tgtgagatgt ttatcatcca cgcacgtaaa
    10141 gcctggcttt cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg
    10201 cgtgtgtatc aactgaagcg tgactttccg catctgacaa tgtcgattaa cggtggtatc
    10261 aagtcgctgg aagaggccaa agcacacctg caacatatgg atggcgtgat ggtcgggcgc
    10321 gaggcgtatc agaatccggg tattctggcg gcggtagacc gggagatctt tggttcctcg
    10381 gataccgatg ccgatccggt ggcggtagtg cgcgccatgt atccgtacat tgagcgtgaa
    10441 ctcagccagg ggacgtatct cggccatatt acccggcata tgttgggctt gttccagggt
    10501 attcctggcg cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa agcgggtgca
    10561 gacattaatg tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc
    10621 aaaaagtagt caaattcacc acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc
    10681 aatagattat ttttggcatg attcttgtaa tgccagcaag agatttcata tttgggagag
    10741 catcatgctg gaactacttt ttgtgattgg cttttttgtc atgctgatgg tcaccggcgt
    10801 ttcgttgctg ggcattatcg ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg
    10861 tatgctggca ttgatgatta agttgctgcc gtggttacta ctggcgattg cggtggtgtg
    10921 ggttattaag gcgattaaag caccaaaagt gccgaaatat cagcgttatg accgctggcg
    10981 ttactaaggg attgtgcgga tgatcacaac ctaaggtttt atccttagaa caaaatagga
    11041 attgataatc aaatctgtca ctattgcgcc tctaacagat tcatcgtgct gtaccctaca
    11101 tacagccgaa ctataaaaag aaagggcttc ccaggtggaa gccctatttc ttttatggaa
    11161 tcaacaggct ggaaccttgc gtcgcccggc tttccagaat ctcatgcgca cgctgcgcat
    11221 ccttcagcgg atatttctgc tgctcggcga catcgacctt aatcacaccg ctggcaatca
    11281 aagagaacag ttcattactg gcctcggtta attcctcccg cgtggtgata tagccttgca
    11341 gggaagggcg tgtcacatac aacgagcctt tttgattgag aatgcctaag ttcacaccgg
    11401 taaccgcacc tgatgagttg ccaaaactga ccattaagcc gcggcgttgc aggcaatcca
    11461 gcgaccgttc ccaggtgtct ctgcccacgg aatcgtacac cacgcgcact ttcttaccgc
    11521 cggtgatctc ttttaaccgc tcgaccagat cctcttcacg atagttaata acctgccacg
    11581 cgcccgcttt tagcgcgctc tgcgcttttt gcgcggttcc tacggtgccg ataagtttcg
    11641 cgcccagggc ttttgcccac tggcaggcaa ttaagccaac gccgccagcc gctgcgtgga
    11701 acaggaactg ttcatcgggt ttaatttcat aggttttgcg cagcagataa taaaccgtta
    11761 agcctttcag gaaggatgcc gcagcttgct caaaagaaat tgccgcaggc agaatcgccg
    11821 ctttatccgc attaatgtta tgcacagagc tgtaagcgcc taacgccgac tgcgcataga
    11881 ctacacgatc gcctgcctta atatgcttta caccactgcc gactttactc acgatgcctg
    11941 ccgcttcggt acctaatccg ctgggtagtg atggcggcgg gtaaaggccg ctgcggatat
    12001 acgtgtcgat aaaattgatg ccgatggctt tattttcgac ctggatttca ttctctgccg
    12061 gatcggcagg agtgaactct acggcttgaa gtacttccgg gccaccgtgc ttgtgaaatt
    12121 caattcgtgt tgccatgtgt cctccagaac gtatcgtcag ggtctgcttc atatggtaaa
    12181 gtttcgaccc attctttatc tcggtaactc cattcactat ggcaggaaat aaacccttca
    12241 acaaacagca ggctgaaccc cgcgaacgcg atccacaagt tgccgggctg aaagtgcctc
    12301 cgcactcgat cgaagcggag cagtcggtgt tgggcggttt aatgctggat aacgaacgct
    12361 gggacgatgt agccgagcgt gtggtggcag acgattttta cacccgccca caccgccata
    12421 tctttactga aatggcgcgt ttgcaggaaa gcggtagtcc tatcgatctg attacccttg
    12481 cggaatcgct ggaacgccag gggcaactcg atagcgtcgg tggttttgct tatctggcag
    12541 agctgtcaaa aaatacgcca agtgcggcga acatcagtgc ttatgctgac atcgtgcgtg
    12601 aacgtgccgt tgtccgtgag atgatctcgg ttgcgaatga gattgctgaa gccggttttg
    12661 atccgcaggg gcgtaccagc gaagatctgc tggaccttgc tgaatcccgc gtctttaaaa
    12721 ttgccgaaag tcgtgcaaac aaagacgaag ggccgaagaa catcgccgat gtgctcgacg
    12781 caaccgtggc gcgtattgag cagttgtttc agcagccaca cgatggcgtt accggagtaa
    12841 acaccggtta tgacgatctc aacaaaaaaa ccgctggctt gcagccgtcg gatttgatca
    12901 tcgtcgccgc gcgtccgtcg atgggtaaaa caacatttgc gatgaacctc gtcgaaaacg
    12961 cggcgatgtt gcaggataaa ccagtactta tcttctcgct ggagatgcct tcagaacaga
    13021 ttatgatgcg ttctctggcg tcgctgtcgc gcgttgacca gactaaaatc cgtaccgggc
    13081 agctcgatga tgaagactgg gcgcgcattt ccggcaccat gggtattttg ctcgaaaaac
    13141 gcaatatcta tatcgatgac tcctccggcc tgacgccaac ggaagtgcgt tcccgcgcac
    13201 gccgtattgc ccgtgaacac ggcggcatcg ggcttatcat gatcgactac ctgcaactga
    13261 tgcgcgtacc ggcgctttcc gataaccgta cgctggaaat tgcagaaatc tctcgctcgc
    13321 tgaaagcact ggcgaaagaa ctgaacgtgc cggtggtggc gctgtcccag ttgaaccgtt
    13381 ctctggaaca acgtgccgac aaacgcccgg tcaactccga cctgcgtgaa tctggctcta
    13441 tcgagcagga tgcggacttg attatgttta tctatcgtga tgaggtgtat cacgaaaaca
    13501 gtgatttaaa aggcatcgcg gaaattatta tcggtaaaca acgtaacggc ccaatcggga
    13561 cggtacgcct gacctttaac ggtcaatggt cgcgcttcga caactatgcg gggccgcagt
    13621 acgacgacga ataataatta ttttatgaat taggtaatta aagcaaacac ttatcaagga
    13681 acacaaatgc aagcggcaac tgttgtgatt aaccgccgcg ctctgcgaca caacctgcaa
    13741 cgtcttcgtg aactggcccc tgccagtaaa atggttgcgg tggtgaaagc gaacgcttat
    13801 ggtcacggtc ttcttgagac cgcgcgaacg ctccccgatg ctgacgcctt tggcgtagcc
    13861 cgtctcgaag aagctctgcg actgcgtgcg gggggaatca ccaaacctgt actgttactc
    13921 gaaggctttt ttgatgccag agatctgccg acgatttctg cgcaacattt tcataccgcc
    13981 gtgcataacg aagaacagct ggctgcgctg gaagaggcta gcctggacga gccggttacc
    14041 gtctggatga aactcgatac cggtatgcac cgtctgggcg taaggccgga acaggctgag
    14101 gcgttttatc atcgcctgac acagtgcaaa aacgttcgtc agccggtgaa tatcgtcagc
    14161 cattttgcgc gcgcggatga accaaaatgt ggcgcaaccg agaaacaact cgctatcttt
    14221 aatacctttt gcgaaggcaa acctggtcaa cgttccattg ccgcgtcggg tggcattctg
    14281 ctgtggccac aatcgcattt tgactgggtg cgcccgggca tcattcttta tggcgtctcg
    14341 ccgctggaag atcgctccat cggtgccgat tttggctgtc agccagtgat gtcactaacc
    14401 tccagcctga ttgccgtgcg tgagcataaa gccggagagc ctgttggtta tggtggaacc
    14461 tgggtaagcg aacgtgatac ccgtcttggc gtagtcgcga tgggctatgg cgatggttat
    14521 ccgcgcgccg ctccatccgg tacgccagtg ctggtgaacg gtcgcgaagt accgattgtc
    14581 ggacgcgtgg cgatggatat gatctgcgta gacttaggtc cacaggcgca ggacaaagcc
    14641 ggggatccgg tcattttatg gggcgaaggt ttgcccgtag aacgtatcgc tgaaatgaca
    14701 aaagtgagcg cttacgaact tattacgcgc ctgacttcaa gggtcgcgat gaaatacgtg
    14761 gattaatcgt tctgtaatat ttgattgtct gtgccggatg cggcgtgaat gccttatccg
    14821 gccaataaaa tcctaaaaat tcaataagtt gatgttcttt catgctctta taaaggtcgt
    14881 gcctctggcg gatgttcgtt tgtcatgagt ctcactctgt tgctaattgc cgttcgctcc
    14941 tgaacatcca ctcgatcttc gccttcttcc ggtttattgt gttttaacca cctgcccgta
    15001 aacctggaga accatcgcgt gtttcaaaaa gttgacgcct acgctggcga cccgattctt
    15061 acgcttatgg agcgttttaa agaagaccct cgcagcgaca aagtgaattt aagtatcggt
    15121 ctgtactaca acgaagacgg aattattcca caactgaaag ccgtggcgga ggcggaagcg
    15181 cgcctgaatg cgcagcccca tggcgcttcg ctttatttac cgatggaagg gcttaacagc
    15241 tatcgccatg ccattgcgcc gctgctgttt ggtgcggacc atccggtact gcaacaacag
    15301 cgcgtagcaa ccattcaaac ccttggcggc tcaggggcat tgaaagtggg cgcggatttc
    15361 ctgaaacgct acttcccgga atcaggcgtc tgggtcagcg atcctacctg ggaaaaccac
    15421 gtagcaatat tcgccggggc tggattcgaa gtgagtactt acccctggta tgacgaagcg
    15481 actaacagcg tgcgctttaa tgacctgttg gcgacgctga aaacattacc tgcccgcagt
    15541 attgtgttgc tgcatccatg ttgccacaac ccaacgggtg ccgatctcac taatgatcag
    15601 tgggatgcgg tgattgaaat tctcaaagcc cgcgagctta tcccattcct cgatattgcc
    15661 tatcaaggat ttagtgccgg tatggaagag gatgcctacg ctattcgcgc cattgccagc
    15721 gctggattac ccgctctggt gagcaattcg ttctcgaaaa ttttctccct ttacggcgag
    15781 cgcgtcggcg gactttctgt tctgtgtgaa gatgccgaag ccgctggccg cgtactgggg
    15841 caattgaaag caacagttcg ccgcaactac tccagcccgc cgaattttgg tgcgcaggtg
    15901 gtggctgcag tgctgaatga cgaggcattg aaagccagct ggctggcgga agtagaagag
    15961 atgcgtactc gcattctggc aatgcgtcag gaattggtga aggtattaag cacagagatg
    16021 ccagaacgca atttcgatta tctgcttaat cagcgcggca tgttcagtta taccggttta
    16081 agtgccgctc aggttgaccg actacgtgaa gaatttggtg tctatctcat cgccagcggt
    16141 cgcatgtgtg tcgccgggtt aaatacggca aatgtacaac gtgtggcaaa ggcgtttgct
    16201 gcggtgatgt aatgcaggaa agcaggctgg agttacccag cctgcagtga aattaaactg
    16261 tcgtcgcttt cactatttct ttgtagatga tttttttgat gccgttgttc tacgtgagag
    16321 ataataaaac gttgtcagtt cttttattgt taagtttatc ccaattatct ggaattcctt
    16381 tccctatttt ttcgtgtgga gtaataattt ctgcacatct aaccattatt aaatttaata
    16441 ataagtatcg ggtttgttac gatagaatat ttctatatcc tcaagaatat actttgcctt
    16501 attgcctgtg actt
//
LOCUS       DAZOXR010000085        16450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZOXR010000085 DAZOXR010000000
VERSION     DAZOXR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.087"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            326..826
                     /gene="tssB"
                     /locus_tag="ACHUNN_002408"
     CDS             326..826
                     /gene="tssB"
                     /locus_tag="ACHUNN_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR0576767.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            861..1085
                     /locus_tag="ACHUNN_002409"
     CDS             861..1085
                     /locus_tag="ACHUNN_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576768.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1136..2611
                     /gene="tssC"
                     /locus_tag="ACHUNN_002410"
                     /pseudo
     CDS             1136..2611
                     /gene="tssC"
                     /locus_tag="ACHUNN_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2618..3031
                     /gene="tssE"
                     /locus_tag="ACHUNN_002411"
     CDS             2618..3031
                     /gene="tssE"
                     /locus_tag="ACHUNN_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0576769.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3035..4885
                     /gene="tssF"
                     /locus_tag="ACHUNN_002412"
     CDS             3035..4885
                     /gene="tssF"
                     /locus_tag="ACHUNN_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0576770.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4849..5931
                     /gene="tssG"
                     /locus_tag="ACHUNN_002413"
     CDS             4849..5931
                     /gene="tssG"
                     /locus_tag="ACHUNN_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR0576771.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5956..7236
                     /gene="tagH"
                     /locus_tag="ACHUNN_002414"
     CDS             5956..7236
                     /gene="tagH"
                     /locus_tag="ACHUNN_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR0576772.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7233..7757
                     /gene="tssJ"
                     /locus_tag="ACHUNN_002415"
     CDS             7233..7757
                     /gene="tssJ"
                     /locus_tag="ACHUNN_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR0576773.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7760..9089
                     /gene="tssK"
                     /locus_tag="ACHUNN_002416"
                     /pseudo
     CDS             7760..9089
                     /gene="tssK"
                     /locus_tag="ACHUNN_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            9094..9855
                     /gene="icmH"
                     /locus_tag="ACHUNN_002417"
     CDS             9094..9855
                     /gene="icmH"
                     /locus_tag="ACHUNN_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR0576774.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9864..12647
                     /gene="tssH"
                     /locus_tag="ACHUNN_002418"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             9864..12647
                     /gene="tssH"
                     /locus_tag="ACHUNN_002418"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12644..13387
                     /gene="vasI"
                     /locus_tag="ACHUNN_002419"
     CDS             12644..13387
                     /gene="vasI"
                     /locus_tag="ACHUNN_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR0576775.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13392..14804
                     /gene="tssA"
                     /locus_tag="ACHUNN_002420"
     CDS             13392..14804
                     /gene="tssA"
                     /locus_tag="ACHUNN_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR0576776.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14823..>16450
                     /gene="tssM"
                     /locus_tag="ACHUNN_002421"
     CDS             14823..>16450
                     /gene="tssM"
                     /locus_tag="ACHUNN_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFR0576777.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         3782 a         4546 c         4554 g         3568 t
ORIGIN      
        1 caagcaatca ttagattagc ttatttttaa tgaaattatt atatacttca ataagttaca
       61 aaaaaactct atatttaaat tattgccata atacacctga agcgaattgc acaggttcac
      121 acaatctatt taacgcttgc agaatacccc tctttacgat cgataaaaag tccttttctt
      181 atttattttg aagcgtaaaa tgcggggtga tgaaggagtg gtgattttat tttcaccgcc
      241 attagggcgg cattgccgat atttttttgc ggtacaaata tgccgtgttc atggcctgta
      301 cgagataaca tgacggatca cttttatgag caaaaaattt gaaggcagcg tcgcgccccg
      361 cgagcgtatt aatatatcgt atgtcccaaa aacagatggc cagacggctg aagtggaact
      421 tccgcttaac atgctcgttg tcggcgatac cggaaatacg caggaaacat ccccactgga
      481 tgaacgtcag gcagtttccg ttaacaagca caatttcggt gcagtgatgg ctgaagcagc
      541 cattggcctc aattttactg tccccgccac gctgaaggga agcacaacag acgatgaact
      601 gaatgtggcg ctgaatatca aatcactgga tgatttttca ccagatagcg tggcccggca
      661 ggttccggaa gtgaataaat tgctggaact gcgtgaagca ctgactgcac tgaaggggcc
      721 gatgggtaac cttccggcat tccgtaccca gctccaggca ttgctggaaa atgaagagtc
      781 ccgcgagcag ttgctcaaag aaatcggtct ggtgagcaat aaataaccac cactatttca
      841 ttaaacaggg ttatccaggc atgacggcac acaaaaatat ctccgggaca taccacctct
      901 ctgttgcaga cattctgcag gtggtttacc aggtatgttt cagcccgtcc gttgaaatca
      961 accaggatgg tgtggctgcg ctgattacaa cactggatcg ccgtatcagt gatctattgg
     1021 atgagattat ccatttttgt gagtttcagc agagcgcgtc gcactggcag cgcgtacttc
     1081 actgatggcg aaaagccggt ttcagaaaca cagctaaaac agaaggattt tcagtatgtc
     1141 tttacaggaa gaggaactcg tttccagtca tgccgggcag cctgagcagg catcgtcgct
     1201 gcttgatcaa atcatggcac agacccgtat tcagcccggt tcggaaggtt atgacgttgc
     1261 cctccagggt gtgacagcct ttattgccag catcctgcaa tccactgcat ctgccgaacc
     1321 ggtcaataaa ctggccgtgg acagcatgat tgcagatatt gatgaacgca tcagccgcca
     1381 gatggatgtc atcatccatg cgcctgcctt tcagcaggtc gagtcgttct ggcgctcact
     1441 gaaaaccatg gtggatcgcg ttgatttccg tgaaaacatc aaggtcaacg tgcttcatgt
     1501 caccaagcag gaacttctgg aagactttga gtttgcgcca gaaattattc agtccggatt
     1561 ttataagcac gtttattcgt ccggtttcgg acagtttggc ggtaaaccca tcgctgccgt
     1621 gctgggcgct tatgagttca aaaataccgc gcctgacatg aaactgctgc aatacgtcag
     1681 tgccgtgggc gcaatggcgc acgcaccgtt cctgtcctcc gtttccccgg agtttatggg
     1741 gctgaactcg tggaccgaac tgcctaatat caaagatctt tatgccatct ttgaaggtcc
     1801 ggcctacacc aaatggcgcg ccctgcgtga ctcggaagat tcgcgctatt tagggcttac
     1861 ggcaccccgc ttcctgcttc gccagcccta ttcaccaaca gataaccctg ttaagaactt
     1921 taactattac gaagatgtca gccagaacca cgaagattat ctgtggggta atacggcctg
     1981 gatgctggca tgcaatatcg cagacagttt tgccaaatac cgctgatgtc caaacattat
     2041 cggtccgcaa agcggcggcg cagtgaaaga tctgccgatg catctgttcg aaacgatggg
     2101 gcagattcag gccaaaatcc caaccgaggt gctggtcacc gatcgccgcg aatttgaact
     2161 ggctgaagag ggttttatca cccttaccat gcgtaaagac tctgataatg cagccttttt
     2221 ctctgcaaac tcggtacaaa aaccgaagca ctttccggga aaagatgcag aaaccaacta
     2281 taagctgggt acgcagcttc cttatctctt catcatcaat cggttagcgc actacatcaa
     2341 ggtgttgcag cgtgaacaac tggggtcatg gaaagaaagg agcgacttag aaagagagct
     2401 taatacctgg atccgtcagt atgttgccga ccaggaaaac ccgcctgctg acgtgcgcag
     2461 ccgcaaacct ctgcgcgctg ccaaagttga ggttatggat gtagaaggcg aacccggctg
     2521 gtaccaggtt gcgctaagcg tgagacctca ttttaaattt atgggggcaa attttgagct
     2581 ttccctggtt ggccggttag acagggagta acacaccatg ataccggatc gggaaagaac
     2641 cacaggttct ctttttgagc gtatggaagc ctcttcggcc agaaaccggc agggaggcag
     2701 tattcattcg ctcaggcaat ccattcgcca gaacctacga aacattctta atacccggtc
     2761 cggtagttgc cggggtgcgc ctgagctggg cattgatgaa ccggaagggg ctgaaaactt
     2821 ccgcgaatcc atgagtcggg ccatcgagca atgtatagaa cgctatgaac cccggatctc
     2881 acacgctgaa gtgcaggcag tggtttcatc cgcctccagc ccgctggata tgacgtttca
     2941 tattacagcc tgggtgacat ttaacgaaac gcatgaggtg cttgaatttg atatggcacc
     3001 gaatggcagc cagcattacc gggtggactg acaaatggaa tttgaagaac gctatttccg
     3061 ggaagaactc gattacctgc gccagcttag caagctgctg gcaacggaaa aaccccatct
     3121 ggcccgcttc ctggccgaaa aagatgcaga tccggatatt gaacgcctgc tggaaggggt
     3181 ggcttttctt accggcaatc tccgccagaa aattgaggat gaattcccag aactgacgca
     3241 cgggcttatt aagatgctat ggcctaatta cctgcgtccg gttccggcaa tgacccttat
     3301 tgaatatacg ccggatatgg ataagtcttc tgtaccggtg ttaatccccc gtaatgagca
     3361 gtttacaacc aacgccgggg aaatcagagt tgatgaagtg ctgccctctg atgctaaaaa
     3421 ggaggagccg cctccctgta ccttcaccct ctgccgggat atctggctgc tgcccgttcg
     3481 cctggagcag attgaaaacc gcagtacgac ccgtaatggt gttatcaaca tcaccttttc
     3541 ggtcgcaccg ggaacggact tccgcacgct ggatctgaac aaacttcgct tctggctcgg
     3601 caatgacgac aactataccc gtgaccagct ttatttatgg ttctgcgaat acttgcaggg
     3661 tgccgacctg actgtgggtg aacagcatat tcgcctgcct gagtttatgc taaaagctgt
     3721 cggttttgag ccgcaggatg ccatgctgcc ctggccgaaa aacgtccaca gcggctaccg
     3781 gatccttcag gagtatttct gttaccccga tgcgtttctc ttttttgatc tttgtggttg
     3841 tccggctttg cctgacggat tgcaggcgga attctttacc ctgcaactgc gtttttcgcg
     3901 ccctttgccc gtggacatcc ggctgcgccg cgattccctg cgcctgtatt gcgcacctgc
     3961 cattaattta tttatccacc atgcagaagc catcacgctg gacaaccggc gggcagacta
     4021 tccgctggtt cccagccgcc attacccaca acattacgat gtattttccg ttaacagtgt
     4081 ggtgagccag gtccaggata tgttcaggaa aaaagatctg gggcgtcctg tttcgacgca
     4141 ggccgcgcgc cagtggccag cctttgaaag tttcagccat cagatagaat acagccggaa
     4201 gcgggaagtg gtgtactggc atcaccggac caaaacatcc ctgttccatc gcggctttga
     4261 tcataccctt gcctttatac atgctgatgg cagttatccg tcagacgaat ctctgctcag
     4321 taatgaagtg gtttcggtat cgctgacctg taccaaccgt gagcttccgt cacaaattcg
     4381 ttccggcgat atcaccggca caaccggaaa aaatgcggct gttgcttcat ttcgcaacat
     4441 tacccgcccg acgcaaccac tctggccggt cattgatggc agcctgcact ggcccctact
     4501 ctccgccatg aacctgaatt atctgtcatt actggatacg gacgcgctga agcaggtcat
     4561 cgccaacttt gatcgccacg caatccatca tccgcagacg gcacggctgt cacaacaaaa
     4621 gctggatgcc attgagcgtc tggagacccg ccccgttgat cgcctgttta cgggtattcc
     4681 cgtccggggg ctggcttcca cgctgtatct gcacccggag ccgtttgtct gtgaagggga
     4741 aatgtatctg ctcggtacgg tgctttcgca ttttctgtcg ctgtacgcca gcgttaactc
     4801 attccacatg ctgaccgttg tgaacacaga aagccaggag acatggaaat ggacggaaag
     4861 aatcgggcag catcctctta tctgagtccg ggaaatcccc cggcagataa agagcagaat
     4921 gatccgctgg cacaggtttt ccataatgcc tgctcttaca atttttttgc gatggcggag
     4981 ctgttgcacc gcctggcaaa gggtgaaaag gggacgccag aattatccct gcgcgacgat
     5041 ccggcacagg aaaccctgcg tttcagcgcc gatgccagcc ttgccttccc gtgcagtgat
     5101 atcagtgcgc tgaaacggga tacgtcaggg gcattcagga tgaccaccac ctttatgggg
     5161 ttacagggga gccagtcgcc tctccccggt tattacctcg ctcacctggc ctggaaagcg
     5221 gtacatgaac aaagtccggt aggtgatttt ctggatatgt tcagccaccg cctgacccag
     5281 tttgtctggc atatctggcg taagtaccgc tatcacatca gttttcgtaa cggcggtgtg
     5341 gatgcgttct cgcagcgcat gtactccctt gtcgggctgg gccaccgcca gcttcgcgat
     5401 aagctggcga ttaatcacag caaaatgctg gcgtattccg ggatcctggc aaatccggga
     5461 cgatccccgg aaattatctg tggcctggtg tcccactgct ttgatttgtc agaagtgaca
     5521 ctgcaaaact ggcaacgtcg caaggtggat attgaaccgg atcagcaaaa tagtctgggg
     5581 agctattctc ttaaaaatgg cgaaaaactg gcgggaagat cggtactggg caactttgtt
     5641 ctgggtacac gagtgcccga tctcagcgga aaattccagc tcagtattac cagcctgacc
     5701 cgaaaacagt tcctctcttt tctgccgtcc ggcgaaaact tcctgccact gacgatgttc
     5761 gtgtccttca tcctgcgcga tcagcttgcc tgggacttac atctggggct ggcgccggag
     5821 caggtgggcg caatgcgcct gggcgataac aaaagtgcgc tgctgggctg gaccagcttc
     5881 ctcggcacac cggaagaacg accctcagtc accatcaggg tacggtcgta aattatgata
     5941 atcacggagc agaatatgcc ggaagagaaa ctacagacgc tttcattgca ggtcattaac
     6001 ggcagtgagc tggaaagcgg gcgggcggcg cgctgtctgt tcacacagca gggaaatgtc
     6061 ggccatggcc ccgaatgcca ctggtcggta caggatcgtc agcagagcat tccggcccag
     6121 gcttttaccg ttatcctgca cgatggcaca ttttgtctgc gcccgcagac ggcacaactg
     6181 tggctgaatc aggcaaaagt cacagcaaca tcagacctaa tacagttgcg ccagggcgat
     6241 gagatccaga tcggacggct gatggtgagg gttcatctga accggggaga tattccccat
     6301 tacgatgagg aaatggccac tcccgaaacc atcgttacca atcgcgatat gctcacggat
     6361 accctgctat caacggaggg tgcgccacac tatccgggaa tgactcaccg gcaccagctt
     6421 gcagacaccg tggtaaacgg tttttctgcc gatccactcc aggctcttca gtccgaaagc
     6481 ctgattacca cgggcgatcc gctttcaggc attgcggctg tccggccatc ggcaccgctg
     6541 tccgatccgg caagtaatgg ggggatcaat actccgttta tggatctgcc gcccatttat
     6601 gccagccccg gcgatcgtaa tgacgacgtt tctgcggcag aaatggcaca acgccacgtt
     6661 gcggtcaccc ccttactgcg cggtcttggc ggctcgctta ccatgagcaa ttccgacgat
     6721 gcggatgatt ttctggagga ggccggacga acgttacagg ccgcaataaa aggtctgctc
     6781 gatttgcagc agcagcgtaa cagcctctca gacaaacatt tgcgcccgct ggaagataac
     6841 ccgctgcgcc tgaacatgga ttacgccacc gcgctcgacg taatgtttgc cgaaggtaaa
     6901 agcccggtac atctggcggc tcccgccgcc gtcagtgaaa gcctgcgcaa tgtccgccac
     6961 cacgaagaag ctaaccgggc agcgattgtg gagtcgcttc gtgtcctgct ggatgctttc
     7021 tcaccacaaa atctgctgcg ccgctttgtg cagtaccgcc gcagccatga actgcgccag
     7081 ccgctggatg atgccggagc atggcaaatg tacagccatt attacgaaga actggcctcc
     7141 gatcgccagc aggggtttga gatgctgttt aacgaggtct acgcccaggt ctatgaccgg
     7201 gtgcttcgtg aaaaacagcg ggagccggaa gcatgaacag gaaggcgttt ctggcctgcg
     7261 tgctgatgtg cgtcatatta accggctgcg aaacggcgaa aaaaatcagt gaggtgatca
     7321 aaaacccgga tatccaggtc gggagcctga aggagcaacc ttcggaaata accgtcaccc
     7381 tgctgaccga accggacacc aataccaatg cggaaggtga atcagccgcc gtggatgtgc
     7441 aactggtgta cctgactgac gactcaaaat tgctggcagc cgactatgac cagatagcca
     7501 gcacgccgct accggatgtg ctgggtaaaa actatatcga ccaccaggat ttcaacctgt
     7561 tgccggacac catcaaaacc ctgccgccgg taaagctgga tgagaaaacg cagtttattg
     7621 gtgtcgtggc ctatttctcc gatgaccagg ccactgaatg gaaacaaatc gagacggtgg
     7681 aaggaatcgg ccaccattac cgcctgctgg tgcatgtacg tcaaagcagc atcgaaatga
     7741 aaaaagagga tgaataatta tggcaaccac ccgcaacaag gtgatgtggc aggaagggat
     7801 gctgatgcgt ccacaccatt tccagcagca gcagcgttac aacgactacc tggataacca
     7861 gcgtttccgg gccatgaatg atttatcctg gggatttacc gaactcaccc tcaacaatga
     7921 actgctggcg cagggtaaga tcatgattga cagcgcgtca ggcacactgc ccgacggcac
     7981 cgtcttttct atccccgacc aggacgcact gcccgatccg ctgcacccgc aaaattttcc
     8041 ggacgaaaga agccgcaata tctacctcgc tctgccggtc gccagtgatg tgagaaatga
     8101 aatcagcgac ggacggcgaa tcgggcgtta ccggctgaat tatgccgatg tccgggattt
     8161 gcattcagaa gaaggtgacg cgcgaacatt gacgctgggt caactgacgc cgcgcattat
     8221 gagcggtgca gaagatatga gcgcctatat tacgctcccg ctttgccgta tcagcgatcg
     8281 ccgccgatgg ctccctgacg ttggatgacg attttatccc ctcctgccag aatattcagg
     8341 tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg ggccattggc ggacgggcaa
     8401 gcgatctggc aaaccgtatt ggctctccgg cgcagagcgg catcgcggat gtggcggaat
     8461 ttatgatgtt gcagttactt aaccgtaacc agacccggtt tacccatcac gcccgtcgat
     8521 cccagctcca cccggaagat ttctaccttg atcttgccgg gttgctgggt gaactgatga
     8581 cctttaccga gccgtcgcgc ctgccctgcc cgcttgatgt gtatgatcat catgacctga
     8641 ccaaaatatt taaaacgctg ttaccggaag tcaaacgggc gctgcatacc gtactgtcgc
     8701 caagagccgt caatctgccg ctgcatctgc gtgacggtat ctggcaggcc gatatccatg
     8761 acacagaact gctgcaatct gccacctttg tgctggccgt ggcggcaaac atgccggtcg
     8821 atcagatcca gcgccagttt atccagcagt cgaaaatttc ctcgccggaa aaaatccgca
     8881 atatggtcag tgtgcagatc cccggtattc cattgcgtgc ccttatggtg gctccccgcc
     8941 agcttcctta ccattccggg ttcagctatt tcgaactcga caagagtggc caggcctgga
     9001 cagaaatggc tgccgccggg gccgttgcac tgcatgtttc cggcagtttc ccggatctga
     9061 acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact gtctctgcca
     9121 ccatttaccg gacacgacga aaaaagccag cgcaactacc atctcgcgct tcgtggcaac
     9181 agcctgaatc cgatgatcga cgcggccacg ccgctactcg gtatggtaat gcgcctttca
     9241 accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac agatgttcag
     9301 gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat ctcgtttcgc
     9361 tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc aaacaagaac
     9421 gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg tggcgagaaa
     9481 gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga tctgctggaa
     9541 tttctctgga tctgtttttc tctgggattt cgcgggcgct acaaagtggc agcacaggat
     9601 cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa actgcgcggc
     9661 gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg ttatcagctc
     9721 atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct ggcgttgttt
     9781 tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca ccagctaaac
     9841 aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg gtaaaacggc
     9901 tgaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg agtcagcagg
     9961 cagcggaaat taccgtcagc catgtgctca ttcagatgct cgccatgcca cgcagtgacc
    10021 tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag gcgctgacgg
    10081 tagagaacta cacaaccgcc cgttctgcgg acagctaccc ggcgttttcc ccgatgctgg
    10141 ttgagtggct taaagagggc tagctgctgg cgtcggctga gatgcagcac agcgaactgc
    10201 gcggcggcgt gttgctgctg gccctgctgc attcgccgct gcgttatata ccgcctgctg
    10261 ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt gtgcagtgga
    10321 cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca ggcacaatga
    10381 cggacgcctc tgacaccctg cttgcccgct atgccaaaaa catgaccgca gacgcccgta
    10441 acggcaggct tgacccggta ctgtgccgcg accacgaaat cgacctgatg atcgacattc
    10501 tctgccgccg ccgtaaaaac aacccagtgg tggtgggcga agcgggcgtg ggcaaaagcg
    10561 cactgattga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac aagctgaaaa
    10621 acaccgatat catgaccctt gacttgggcg cattgcaggc cggggcgtcg gtgaagggtg
    10681 aattcgaaaa acgtttcaaa gggctgatgg cggaggtcat ttcctccccg gtgccggtca
    10741 ttctgtttat cgacgaagca cataccctga ttggcgcggg caaccagcag ggcgggctgg
    10801 atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga gctgaaaacc atcgccgcca
    10861 ccacctggag cgagtacaaa aaatacttcg aaaaagatgc cgccctgtcg cgccgcttcc
    10921 agttggtgaa ggtcagcgaa cccaacgctg ccgaagccac cattattctg cgcggtctgt
    10981 cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg caggccgctg
    11041 cgacattaag cgagcgttat ctctccgggc gtcagttacc ggacaaagcg attgatgtgc
    11101 tggataccgc ctgcgcccgt gtggccatca acctgtcgtc gccgccgaag caaatctcgg
    11161 cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag cgcgagcttc
    11221 gcatcggact gcgtaccgac acatcacgga tgaccgaggt gctggtgcag tatgatgaaa
    11281 cgctgacggc gctggatgaa ctggaagcgg cctggcacca gcagcagacg ctggtccggg
    11341 agattatcgc gctgcgccag cagttactgg gcgtggcaga ggacgatgcg gcgccgttgc
    11401 cggacgcaga taccgtggag gatacgcagc cagagtcaga gtcagagtca gagtcagagt
    11461 cagagcagga taataccggt gccgaaccgg ctgatgaggc cgacagagaa caaccggaag
    11521 agaccgctga aacagtttcc ccggtacagc ggctggccca gctcactgcc gaactggacg
    11581 ccctgcataa cgaccggttg ctggtctccc cgcacgtcga taaaaaacag attgcggcgg
    11641 tgattgccga atggaccggc gtgccgctca accgcctgtc gcagaatgaa atgtcggtca
    11701 tcaccgacct gacgaaatgg ctgggtgaca ccatcaaagg ccaggacctg gcgattgcca
    11761 gcctgcataa gcatctactg accgcacgcg ccgacctgcg tcgtccggga cgcccgctcg
    11821 gtgcgttcct gctggctggc cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg
    11881 cagagctgct ctacggcggt cgccagtacc tgaccaccat caatatgtcc gaattccagg
    11941 agaaacacac cgtctcgcgg ctgattggtt cgcctccggg ctacgttggc tacggtgaag
    12001 gcggcgtact gaccgaagcg attcgccaga aaccctactc ggtagtactg ctcgatgaag
    12061 tggaaaaagc gcacccggat gtgctcaacc tgttctacca ggcgttcgat aagggcgaaa
    12121 tggcagacgg tgaaggccgc ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca
    12181 acctcggcta ccaggtaata gtcgagcatg ccgatgaccc ggaaaccatg caggaagtac
    12241 agtatccggt gctggccgac ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc
    12301 cgtatctgcc gctgtcgaaa gagacgctcg ccaccattat cgccggaaaa ctggcccgtc
    12361 tggataacgt gctgcgcagt cgctttggtg tagaagtggt cattgaaccg gaagtgacgg
    12421 acgaaatcat gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca
    12481 tcgatggcga catgctaccg ccgctctcgc tgctgctgtt gcagaaaatg gcggctaaca
    12541 cggcgattgc ccggattcgg ttgtcggcag tggacggcgc atttacggca gacgtggaag
    12601 atgctcagaa cgacgagtcc gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt
    12661 tttccggcag acaggatggt ttatcgcggg cctgatgacg ggcttacccg ccactgccgc
    12721 gcccgcagaa gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc
    12781 ggatgcgaca gccacgttac aggccatgca gtcctgtcgg cgggagtccg cggcgctgga
    12841 gcggcttgac tgctatgacc gcctgctggc accgctgtcc ccgtcagggt ttgacggggc
    12901 gctggtcaaa gccggttttg tgggcgaggc ctggacccgc gccacagagc aggaaaaacg
    12961 ccgcgagggc aacaccacag aactgctggt gacgcaggtg ccgggggagc gcccgacggt
    13021 ggtgataacc accccggcta tcggtcatgt tccgccacgc ccggtgctga tgttcagttg
    13081 cgtggacaat atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat
    13141 cgccgtcact ctgaatgccg acaaccgggc attgcgcagc cactggtttg tgcgcgagaa
    13201 cggcaccctg ctggagtcca gccgggggtt atccgggatt gatgaaatta agcaactgtt
    13261 cggcgcaaaa acgctgacgg tggataccgg cgcagacaat gccgccggaa agctgacctt
    13321 taacattgac gggctggcaa gggcgattgc tcccctgcgc gatgcctgcc actgggcggg
    13381 agaataaaaa tatggcaatg gacttacgcg atccgaatgt ctggatatcg cacctgctgg
    13441 aaaacctgcc ggaagagaaa ctggcatcgg cgctgaaaga tgacaacccg aactgggagt
    13501 atatcgacgg cgaaatcgtc aagctgggat cgctggccca cagtcaactg gatatcccgg
    13561 aactccagcg ccgggggctg gtcattctgg cttcggagag taaggatttt cgtctgctgg
    13621 cgcatctgct aagaaccctg caacacgccg gtgatcctca tctcgcattg cgcctgctgg
    13681 cgctgtatgt ggagcattac tgggcggtgg ctgcgccgca gaatatggcg cataaaaaac
    13741 gctttgccag ccaggtcatt aaacgctttg aaacaggtat tgaagttttt tcacagaacg
    13801 ccgccacagc gcagcgcgat gcgctgtcag gggaactggc aaaactggcg caatgctggc
    13861 agtcacataa cgccccggaa ctggcacagg ctactgatga tctttttgcc ctgtaccagc
    13921 gtgcgtttaa tcgtgcggca ccggctcctg tccccactcc ggcggcctcc ggtagttcac
    13981 cacagaccac cgccacgtct gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa
    14041 tcgtcatcga cagtcacgac gacaaagcct ggcgcgacac gctgttaaaa gtggcggcca
    14101 ttttatgtga acgccagccg gattcaccgc agggctaccg tctgcgccgc catgcgcttt
    14161 ggcagaacat caccagtacg ccccaggcgg aaagcgatag ccgtactcca ctggctgcgg
    14221 tctccgccga tatggtggca gattaccatg ctcagctcgg tagtgccgat atggcgctgt
    14281 ggcagcaggt ggagaaaagc gtactgctgg ctccttactg gctggatggt cactgccttt
    14341 ctgcacagac ggcgctgcgt ctgggttaca aacaggtggc agacgccatc cacgatgagg
    14401 tcatccgctt ccttgagcgt ctgccccagc ttaccgggct gctgtttaat gaccacacac
    14461 cgtttatcag tgagcagacg aaacaatggc tggcagcttc gcccgacgcc aaagttgcac
    14521 cggttgcgca aatcggtgag gaatcgaagg cagccagagc ctgttttgct gagcagggtc
    14581 tggaggcggc gctgcgatat ctggacatgc tacccgaagg cgatccccgc gatcagtttc
    14641 accgccagta ccttgccgca cagttgacgg aagaggcggg gctggtacag cttgcgcagc
    14701 agcagtaccg gatgttgttc aggatgggat tgcagatgat ggtggcagac tgggagccgt
    14761 ccctgcttga gcagctagaa caaaaattca cggcagaaca ataaaagaga acaggagttt
    14821 ttgtgttcag attaccaaca ccccgactac tcagcggact caaatcagcc ctgcgaccgg
    14881 cgatgccccg gtttaaagtc tctgctttct ggctgctgat actggcgtgg atttttctgc
    14941 tggtgtggat ctggtggaaa ggcccgatgt ggacgctgta tgaagaacag tggctcaaac
    15001 cactggcgaa ccgctggctg gcaacggcgg cgtgggggat tattgccctg gtgtggctta
    15061 ccgtccgggt gatgaagcgc ctgcaacagc ttgaaaaaat gcaaaagcaa cagcgcgagg
    15121 aagccgttga tccgctcagc gtggaactga acgcccagca gcgttatctt gaccgctggc
    15181 tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct gtggcagttg ccgtggtata
    15241 tggtcatcgg cccggcgggc agtggcaaaa cgacactgct gcgcgagggg tttccgtccg
    15301 acattattta tgccccggag ggcgcacgcg gcgcagaaca acgcctgtac ctcacgcccc
    15361 atgtcggtaa acaggcggtg atctttgata tcgacggcac actctgcgct cccgctgatg
    15421 cggatatcct gcatcgccgt ctgtgggaac atgccctcgg ctggctgaaa gaaaagcgcg
    15481 cgcgccagcc gctgaacggg attattctga cactcgattt acccgatctg ctgaccgcag
    15541 acaaacgccg ccgcgagcat ctgttacaga cgctgcgcag ccgcttacag gatatacgcc
    15601 agcatcttca ctgccagtta ccggtttacg tggtactgac ccggcttgat ttattacagg
    15661 gttttgccgc cctgttccag tcactcaaca gacaggatcg cgatgcgatt ctgggcgtca
    15721 cgttcacccg ccgtgcccat gaaaatgatg actggcgaac agagttgaat gctttctggc
    15781 agacatgggt ggatcgaatg aatctggcgt tgccggatct gatggtcgct cagactcaca
    15841 cccgcgcgtc tttattcagt ttttcccgcc agatgcaggg aagccgtgaa ccgctggtgt
    15901 cactgcttga gggtctgctt gatggcgaaa atatgaacgt gatgctgcgt ggtgtctatc
    15961 tcacctcttc gcttcagcgt ggacagatgg atgatatatt cacccagtct gccgcccgcc
    16021 agtaccggct gggcaataac ccactggcgt cctggcccct ggtggacacc gcgccttatt
    16081 tcacccgcag cctgttcccg caggcattac tcgcagagcc taatctggca acagagagcc
    16141 gcgcctggct gatacgttcc cgtcgccgcc tgacggtttt ctctgccaca ggcggcgtgg
    16201 cagcactgct gctcatcacc ggctggcatc actattacaa cggtaactat cagtccggca
    16261 tcaccgtgct taagcaggcc aaagccttta tggacgtgcc gcctccgcag ggggaagatg
    16321 actttggcaa cctgcaactg ccgctcctga acccggtacg cgatgccaca ctggcctatg
    16381 gcgactgggg cgaccgcagc cgtctggccg atatgggact gtaccaggga cgacgtatcg
    16441 ggccttatgt
//
LOCUS       DAZOXR010000086        16224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZOXR010000086 DAZOXR010000000
VERSION     DAZOXR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.088"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="ACHUNN_002422"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="ACHUNN_002422"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR0576778.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="ACHUNN_002423"
     CDS             complement(2362..2802)
                     /locus_tag="ACHUNN_002423"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0576779.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUNN_002424"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUNN_002424"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0576780.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="ACHUNN_002425"
     CDS             3520..4218
                     /locus_tag="ACHUNN_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0576781.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="ACHUNN_002426"
     CDS             4447..5655
                     /locus_tag="ACHUNN_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0576782.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUNN_002427"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUNN_002427"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0576783.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="ACHUNN_002428"
     tRNA            7762..7838
                     /locus_tag="ACHUNN_002428"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            8583..10190
                     /gene="dppA"
                     /locus_tag="ACHUNN_002429"
     CDS             8583..10190
                     /gene="dppA"
                     /locus_tag="ACHUNN_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0576784.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10498..11517
                     /gene="dppB"
                     /locus_tag="ACHUNN_002430"
     CDS             10498..11517
                     /gene="dppB"
                     /locus_tag="ACHUNN_002430"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0576785.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11527..12429
                     /gene="dppC"
                     /locus_tag="ACHUNN_002431"
     CDS             11527..12429
                     /gene="dppC"
                     /locus_tag="ACHUNN_002431"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0576786.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12440..13423
                     /gene="dppD"
                     /locus_tag="ACHUNN_002432"
     CDS             12440..13423
                     /gene="dppD"
                     /locus_tag="ACHUNN_002432"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0576787.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13420..14424
                     /gene="dppF"
                     /locus_tag="ACHUNN_002433"
     CDS             13420..14424
                     /gene="dppF"
                     /locus_tag="ACHUNN_002433"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0576788.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14454..15725)
                     /locus_tag="ACHUNN_002434"
     CDS             complement(14454..15725)
                     /locus_tag="ACHUNN_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0576789.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3856 a         4043 c         4355 g         3970 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcaacg
     7861 agcaggcttt tttgcatttg tagctcatga ggatgagaac ctccgggggc agggaggttc
     7921 gactcgacgt aggcctgata agacgcgcca gcgtcgcatc aggcaacggc accgcattta
     7981 ttgtcggatg cggcgcgacc gccttattcg accaacacac cacacatttt cctgccaact
     8041 ttgcgcccca tatctcacta tccattaacg gatttgtgac agaaaccatt gtcttttttt
     8101 atatttgctt aaatcttttt ttgcgttact tatggctggc gctggcattt tccgctgtgc
     8161 gttttagcgt tcatggtatt aatcgctcag atatccacct tttcctcaga tttttttcat
     8221 taaatgcata aaacttaatc accaaatgtt gctaaatatt aagctgattg ttctggttat
     8281 gttggagtag cacaaatttc tctgctgcaa atagctgttt gaagtttttt taatctttgt
     8341 ttgtggattc tcaccgttgg tgtaaatccc ggttggctgt attgacgttt tcacattctg
     8401 ttgacagatt gtaggtcacg aggggcattt tatggaggat ccgcactgtt acactgatgt
     8461 taattagtac ggcatcccca cctcataacg ttgacccgac cgggcaaaaa acaaaaaagg
     8521 tcaggcagcg acaacccact gcaaagggtt aaaacaacaa acatcacaat tggagcagaa
     8581 taatgcgtat ttccttgaaa aagtcaggga tgctgaagct tggtctcagc ctggtggcta
     8641 tgaccgtcgc agcaagtgtt caggctaaaa ctctggttta ttgctcagaa ggatctccgg
     8701 aagggtttaa cccgcagctg tttacctccg gcaccaccta tgacgcctct tccgtaccgc
     8761 tttataaccg cctggttgaa tttaaaatcg gcaccactga agtgatcccg ggcctcgctg
     8821 aaaagtggga agtcagcgaa gacggtaaaa cctatacctt ccatctgcgt aaaggtgtga
     8881 agtggcacga caataaagaa ttcaaaccga cgcgtgaact gaacgccgat gacgtggtgt
     8941 tctcgttcga ccgtcagaaa aacgcgcaaa acccgtacca taaagtctct ggcggcagct
     9001 acgaatactt cgaaggcatg ggcttgccgg agctgatcag tgaagtgaaa aaggtggacg
     9061 acaacacagt tcagttcgta ctgactcgcc cggaagcgcc gttcctcgct gatctggcaa
     9121 tggacttcgc ctctattctg tcaaaagaat atgctgatgc gatgatgaaa gccggtacac
     9181 cggaaaaact ggacctcaac ccaatcggaa ccggtccgtt ccagttacag cagtaccaaa
     9241 aagattcccg tatccgctac aaagcgtttg atggctactg gggcaccaaa ccgcagatcg
     9301 atacgctggt tttctctatt acccctgatg cttccgtgcg ttacgcgaaa ttgcagaaga
     9361 atgaatgcca ggtgatgccg tacccgaacc cggcagacat cgctcgcatg aagcaggata
     9421 aatccatcaa cctgatggaa atgccggggc tgaacgtcgg ctacctctcc tataacgtgc
     9481 agaaaaaacc gctggatgac gtgaaagttc gccaggcact gacctacgcg gtgaacaaag
     9541 acgcgatcat caaagcggtt tatcagggcg cgggtgtatc agcgaaaaac ctgatcccgc
     9601 caaccatgtg gggctataac gacgacgttc aggattacac ctacgatcct gaaaaagcga
     9661 aagccttgct gaaagaagcg ggtctggaaa aaggtttctc catcgacctg tgggcgatgc
     9721 cggtacaacg tccgtataac ccgaacgctc gccgcatggc ggagatgatt caggcagact
     9781 gggcgaaagt gggcgtgcag gccaaaatcg tcacctacga atggggtgag tacctcaagc
     9841 gtgcgaaaga tggcgaacat cagacggtaa tgatgggctg gactggcgat aacggggacc
     9901 cggataactt cttcgccacc ctgttcagct gcgccgcctc tgaacaaggt tctaactact
     9961 caaaatggtg ctacaaaccg tttgaagatc tgattcaacc ggcgcgtgct accgacgacc
    10021 ataacaaacg cgttgaactg tacaagcaag cgcaggtggt gatgcacgat cagactccgg
    10081 cactgatcat cgctcactcc accgtgtttg aaccggtacg caaagaagtc aaaggctatg
    10141 tggttgatcc attaggcaaa catcacttcg aaaacgtctc tatcgaataa ttaaaagcca
    10201 tacaagactg atggcaaagg caaaactgcc tgatgtgctc cgcttatcag gcctacgaaa
    10261 attctgcaat gtattgaatt tgcacgattt tgtaggccgg ataaggcgtt aacgccgcat
    10321 ccggcataaa caaagcgcac tttgtcaaca atctgtatac ccggtggcga tgtgcctctg
    10381 cgtgcagcgc catccggcag caatacttca ttccctgtcc gataagggcg ggaattgatt
    10441 tgtgagcaat acagacacgc agttccaggc tgcgggtcac tatagagaat ccgggttatg
    10501 ttgcagttta ttctccgacg tttgggactc gtcatcccca cgtttatcgg tattaccctt
    10561 ctcacatttg cctttgtcca catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa
    10621 cgcgggatct cccctgagcg tcacgcgcag ctgctggctg aactcggctt agataaaccg
    10681 atgtggcagc agtatctcca ttacatttgg ggcgtaatgc acggcgatct aggcatttca
    10741 atgaaaagcc gaattccagt atgggaagag ttcgtgccgc gctttcaggc cacgctggaa
    10801 cttggcgtct gcgcgatgat ttttgctacg gcagtcggta ttccggttgg tgtgctggct
    10861 gcggttaaac gcggttccat tttcgatcac actgcagttg gcctggcgct gaccggctac
    10921 tcgatgccga tcttctggtg gggcatgatg ctgatcatgc tggtttcggt gcactggaac
    10981 ctgacgcccg tctccggtcg cgtgagcgat atggtgttcc tcgatgactc caatccgtta
    11041 accggtttta tgctgatcga caccgccatc tggggtgaag atggcaactt tatcgacgcc
    11101 gttgcccata tgatcttacc ggctattgtg ctgggtacta tcccgctggc ggttatcgtt
    11161 cgtatgacgc gctcttcgat gctggaagtg ctgggtgaag attacatccg caccgcgcgc
    11221 gccaaagggt tgacccgcat gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg
    11281 ccggtagtga ccgttatcgg cctgcaggtg ggaacattgc tggcgggggc gattctgacc
    11341 gaaaccatct tctcgtggcc cggtctggga cgctggttga ttgacgcact gcaacgccgc
    11401 gactatccgg tagtgcaggg cggcgtattg ctggtggcga cgatgattat cctcgtcaac
    11461 ctgctggtcg atctgctgta cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg
    11521 gacatcatgt cacaggttac tgaaaataaa gtgattagcg caccggtgcc gatgaccccg
    11581 ttacaggagt tctggcacta ttttaaacgc aacaaaggcg cggttgtcgg gctggtttac
    11641 atcgtcatcg tgctgtttat cacgatcttt gccaactgga ttgcgcctta taacccggcg
    11701 gatcagttcc gcgatgcgct gctcgccccg ccagcctggc aggaaggcgg cagcatggcg
    11761 cacttgctgg gcaccgatga cgtaggccgt gatgtgctgt cgcggctgat gtacggcgcg
    11821 cgcttgtcgc tgctggttgg ctgtctggtg gtggtgttat cgctgattat gggcgttatt
    11881 ctcggcctga tcgccggtta cttcggcggc ctggtcgata acatcatcat gcgcgtggtc
    11941 gatatcatgc tggcgctgcc aagtctgctg ttggcactgg tgctggtggc gattttcggc
    12001 ccgtcgattg gtaacgccgc gctggcactg accttcgttg ccttgccgca ctatgtgcgc
    12061 ttaacccgcg ccgccgtgct ggtggaggtg aatcgcgatt acgttaccgc gtctcgcgtg
    12121 gcaggtgccg gggcgatgcg tcagatgttt attaacatct tcccgaactg cctggcgccg
    12181 ctgattgttc aggcgtcgct cggtttctct aacgccattc tcgatatggc cgcccttggc
    12241 ttcctcggca tgggagcgca gccgccaaca ccagagtggg gcaccatgct ctccgacgtg
    12301 ttgcagttcg cgcaaagcgc ctggtgggtc gtgaccttcc cgggtctggc gatcctgctg
    12361 acggtgctgg catttaacct gatgggtgac ggtctgcgtg acgcgctcga tcccaaactg
    12421 aagcagtaag aggttcgaga tggcgttatt aaatgtagat aaattatcgg tgcatttcgg
    12481 cgacgaaagc gcaccgttcc gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga
    12541 agtggtcggg attgtgggtg agtctggctc cggtaagtcg gtcagttcac tggcgattat
    12601 ggggctgatt gattatccgg gccgcgtaat ggcggaaaag ctggtgttta acggtcagga
    12661 tttgcagcgt atatcagaaa aagagcgccg caacctggtg ggtgccgaag tggcgatgat
    12721 cttccaggac ccgatgacca gccttaaccc gtgctacacc gtgggtttcc agatcatgga
    12781 agcgattaag gtgcatcagg gcggtaataa gagcactcgc cgtcagcggg cgattgacct
    12841 gctgaatcag gtcggtattc ccgatccggc atcgcgtctg gatgtttacc cgcatcagct
    12901 ttccggcggc atgagccagc gcgtgatgat cgccatggcg attgcctgtc ggccaaaact
    12961 gctgattgcc gatgagccga ccactgcgct ggacgtgacc attcaggcgc aaatcatcga
    13021 actgttgctg gagctacagc agaaagagaa catggcgctg gtgttaatta cccatgatct
    13081 ggcgctggtg gcggaagctg cgcataaaat cattgtgatg tatgcaggcc aggtggtgga
    13141 aacgggcgat gcgcacgcca tcttccatgc gccgcgtcac ccgtatactc aggcattgct
    13201 gcgtgcgctg ccagaatttg ctcaggacaa agaacgtctg gcgtcgttgc caggtgtcgt
    13261 tcccggcaag tacgaccgcc cgaacggctg cctgcttaac ccgcgctgcc cctatgccac
    13321 tgacagatgt cgcgctgaag aaccggcgct gaatatgctc gctgacgggc gccagtccaa
    13381 atgccattac ccacttgatg atgccgggag gccgacacta tgagtacgca agaggccacc
    13441 ctgcaacaac cgctgttgca ggctatcgac ctgaaaaaac attatccggt gaagaaaggc
    13501 atgttcgcgc cggaacgtct ggttaaagcg ctggatggcg tttcgtttaa ccttgaacgt
    13561 ggcaaaacgc tggcagtagt gggcgaatct ggctgcggta aatcgaccct cggtcggttg
    13621 ctgacgatga ttgaaacgcc caccggtggc gagctgtatt accaggggca ggatctgctt
    13681 aagcacgatc cgcaggcgca gaagctgcgt cggcagaaaa tccagatcgt cttccagaac
    13741 ccttacggtt cgctgaatcc gcgtaaaaaa gtcgggcaaa ttcttgaaga gccgctgctg
    13801 atcaacacca gcttaagcaa agaacagcgt cgggaaaaag ccctgtcgat gatggcgaaa
    13861 gtcggcctga aaaccgagca ctatgaccgc tatccgcata tgttctccgg cggtcagcgt
    13921 cagcgtatcg ccatcgcccg tggtctgatg ctcgacccgg atgtggtgat tgccgatgaa
    13981 ccggtttccg cgctggatgt ttcagtgcgc gcgcaggtgc tgaatctgat gatggatttg
    14041 cagcaggagt tggggctgtc ttatgtcttt atctcccacg acctgtcggt ggtggagcac
    14101 attgctgatg aagtgatggt gatgtacctg ggccgctgcg tggagaaggg aacgaaagac
    14161 caaatcttca ataacccgcg ccatccgtac actcaggcgc tactttccgc gacgccgcgc
    14221 ctgaacccgg acgatcgccg cgagcgcatc aagctcagcg gtgaactacc aagcccactg
    14281 aatccaccgc cgggttgcgc cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc
    14341 cagttgcagc cgcagctaaa agactacggc ggtcaactgg tagcttgttt tgctgttgat
    14401 caggatgaaa atccgcagcg ttaactcaca aaaccggggc catgcgctcc ggtttactca
    14461 atcagcttca acagtggcga caagcatagc aaaatgccgt acagcagaat cagccaggct
    14521 ttaaatccgc gaagtttttc cagttgtgcg actttataga tcaggaaaaa cggaatgagg
    14581 cacgacacaa taccatataa cgggcttccc aactgaaaga acaccagcac cgatacacga
    14641 aacgaaaccc aaatcgtcaa cgtaatgacg ataaaagcgc agatcgccag agtcagcacg
    14701 cgtgagttaa ttttcttggt atcaataatt cggcttaaca gattgagaat aatgccttta
    14761 atggcctcgt ggaaaccgag ataaatgcca aagaatgcgg tcagtacggc aaagatatta
    14821 agcaccgtag aggtgatatg aatgatatgc ccagggatca cctgcgcggc cagcgccagt
    14881 gctgagatat tttgttcaaa ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac
    14941 gaaaaggcaa aaaacaggat caccgcgatg agcgtgatat aactaattcg gtgggtacgc
    15001 agcgcgagcc gggttgccag tactttatcc gcttcccgtt tacgataggc aatattcatt
    15061 gggttaagta cctgaataaa tactgcagaa aagaagcaaa atggaatggt aagcaagaca
    15121 tcgcggaaaa agacggaggc ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg
    15181 atcatcgcaa aaccgaacac gacaataatc cctactttga ccaccaccat tggcccggaa
    15241 atcttaaaca gtaatcgttc accaccagac gcaatcgcta ccagtacggc gaaaatagcg
    15301 actttataaa gtagagattg tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac
    15361 gaggcgctgt cgaaaaccac ggagagagag tagataaaaa tcccgtggat aatcatcaaa
    15421 aagtagataa ccccgaggaa aattccccag ttcttcccca ggtaatgact gataatatcg
    15481 gtgtagtcat tacaggaatc gctttcagaa agggttttta aataaatgtc ctgcactacc
    15541 caggtggcag gataagcaat gatcgctgcg gtaataaata cccaaattcc cttcaagcca
    15601 atttgtactg gcatcagcac ggttccggca ccaatcgcca tgcctatgca taataaaacc
    15661 cagccgaagt cgtagcgtgt aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc
    15721 tgcataaagt cctgtccaga aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc
    15781 ttgcgcaaca ggtggagaag tcgaatacgc agtatgtaaa aaatgaggca acagattcac
    15841 tggggaggtc gcgaggaggc gcaattctac aaaattgcgc atttcactac cagtctggag
    15901 tcagcccgga tatgaactgc aactattgca aataaagaaa ttaagctgga aggcatcgcg
    15961 caatgaggaa gattgccggg tgctgacagt aaaaagcaat cgtaaaatca gcggcaagcc
    16021 gaaagggttc cggtgaggcg caatgttgcg ggggctttat ccctggtggc attggttgct
    16081 ggagagagaa aacccccgca cgttgcaggt atgtacctga caacataacg ggggctaatc
    16141 ttgaacctag accactcaag aatagccgcg aaacgttgtc attacaacac aggcggctat
    16201 atgacgctcg cagagctggg catg
//
LOCUS       DAZOXR010000087        15963 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZOXR010000087 DAZOXR010000000
VERSION     DAZOXR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.089"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUNN_002435"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUNN_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0576790.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUNN_002436"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUNN_002436"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0576791.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUNN_002437"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUNN_002437"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR0576792.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUNN_002438"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUNN_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUNN_002439"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUNN_002439"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0576793.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUNN_002440"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUNN_002440"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR0576794.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUNN_002441"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUNN_002441"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0576795.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHUNN_002442"
     CDS             8229..8939
                     /locus_tag="ACHUNN_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0576796.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUNN_002443"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUNN_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0576797.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHUNN_002444"
     CDS             9636..10343
                     /locus_tag="ACHUNN_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0576798.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHUNN_002445"
     CDS             10343..10891
                     /locus_tag="ACHUNN_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0576799.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHUNN_002446"
     CDS             10901..12067
                     /locus_tag="ACHUNN_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0576800.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHUNN_002447"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHUNN_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHUNN_002448"
     CDS             13576..14229
                     /locus_tag="ACHUNN_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0576801.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUNN_002449"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUNN_002449"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR0576802.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15963)
                     /locus_tag="ACHUNN_002450"
     CDS             complement(15612..>15963)
                     /locus_tag="ACHUNN_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0576803.1"
                     /translation="LFYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAA
                     KHQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTR
                     VWSGYLTLKSLQRQ"
BASE COUNT         3701 a         4345 c         4163 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 aga
//
LOCUS       DAZOXR010000088        15703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZOXR010000088 DAZOXR010000000
VERSION     DAZOXR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.090"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(75..794)
                     /gene="deoD"
                     /locus_tag="ACHUNN_002451"
     CDS             complement(75..794)
                     /gene="deoD"
                     /locus_tag="ACHUNN_002451"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0576804.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(874..2097)
                     /gene="deoB"
                     /locus_tag="ACHUNN_002452"
     CDS             complement(874..2097)
                     /gene="deoB"
                     /locus_tag="ACHUNN_002452"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0576805.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(2149..3471)
                     /gene="deoA"
                     /locus_tag="ACHUNN_002453"
     CDS             complement(2149..3471)
                     /gene="deoA"
                     /locus_tag="ACHUNN_002453"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0576806.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(3598..4377)
                     /gene="deoC"
                     /locus_tag="ACHUNN_002454"
     CDS             complement(3598..4377)
                     /gene="deoC"
                     /locus_tag="ACHUNN_002454"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0576807.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            4635..6185
                     /locus_tag="ACHUNN_002455"
     CDS             4635..6185
                     /locus_tag="ACHUNN_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0576808.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            6157..7020
                     /locus_tag="ACHUNN_002456"
     CDS             6157..7020
                     /locus_tag="ACHUNN_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0576809.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(7536..8318)
                     /locus_tag="ACHUNN_002457"
     CDS             complement(7536..8318)
                     /locus_tag="ACHUNN_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0576810.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8315..9388)
                     /locus_tag="ACHUNN_002458"
     CDS             complement(8315..9388)
                     /locus_tag="ACHUNN_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0576811.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(9510..9671)
                     /locus_tag="ACHUNN_002459"
     CDS             complement(9510..9671)
                     /locus_tag="ACHUNN_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0576812.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(9798..10403)
                     /gene="osmY"
                     /locus_tag="ACHUNN_002460"
     CDS             complement(9798..10403)
                     /gene="osmY"
                     /locus_tag="ACHUNN_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0576813.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(10796..12385)
                     /gene="prfC"
                     /locus_tag="ACHUNN_002461"
     CDS             complement(10796..12385)
                     /gene="prfC"
                     /locus_tag="ACHUNN_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0576814.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(12476..13153)
                     /gene="yjjG"
                     /locus_tag="ACHUNN_002462"
     CDS             complement(12476..13153)
                     /gene="yjjG"
                     /locus_tag="ACHUNN_002462"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0576815.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(13168..13614)
                     /gene="rimI"
                     /locus_tag="ACHUNN_002463"
     CDS             complement(13168..13614)
                     /gene="rimI"
                     /locus_tag="ACHUNN_002463"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0576816.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(13583..13996)
                     /gene="holD"
                     /locus_tag="ACHUNN_002464"
     CDS             complement(13583..13996)
                     /gene="holD"
                     /locus_tag="ACHUNN_002464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0576817.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            14099..15130
                     /gene="rsmC"
                     /locus_tag="ACHUNN_002465"
     CDS             14099..15130
                     /gene="rsmC"
                     /locus_tag="ACHUNN_002465"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0576818.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            15398..15484
                     /locus_tag="ACHUNN_002466"
     tRNA            15398..15484
                     /locus_tag="ACHUNN_002466"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15432..15434,aa:Leu,seq:cag)
     gene            15513..15599
                     /locus_tag="ACHUNN_002467"
     tRNA            15513..15599
                     /locus_tag="ACHUNN_002467"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15547..15549,aa:Leu,seq:cag)
BASE COUNT         3621 a         4256 c         4125 g         3701 t
ORIGIN      
        1 tgaatagata ctaaaaagcc ggagcagtcc cccactccgg cttaacacga caatcgcctt
       61 tcagcgaaac acaattactc tttatcgccc agcagaacgg attccagtgc gattttgatc
      121 atgtcgttga aggtggtctg acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg
      181 tcagacacag tgcagatggt cagggctttc gcgccaaatt ctgcagcgac gccgtagata
      241 ccagccgctt ccatttccac gccgaggatg ccgtattttt ccatcacgtc gaacatttcg
      301 ccgtccggag agtagaacag gtcagcggag aacaggttac caacgcgagc atcaatgccc
      361 agtgctttag ccgcgtctac cgcgttacgc accatgtcaa agtcagcgat agcggcaaag
      421 tcatgatctt taaaacggat gcggttaact ttggaatcgg tgcaggcacc cataccgata
      481 acgacgtcgc gcagttttac gtgcggcaga actgcgccac aggaacccac gcggataatt
      541 ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga tggagcagga cgggataccc
      601 ataccgtgac ccattacgga aattttgcgg cctttgtaag taccggtgaa gcccagcata
      661 ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag tttcagcaat atacttcgca
      721 cgcagcgggt cgcctggcat caaaactacg tcagcgaaat cgcccatttc tgcattaatg
      781 tgtggggtag ccattgtttt atccttatca acatgttttc accctctccc tgagggagag
      841 ggccggggtg agggcatcaa gccgcaccca ttatcagaac atggctttgc catattccat
      901 atcagaagta ccaaaatatt ttgccagagt ctggccgata tccgcgaagg tttcacggtg
      961 acccagtgag cccggtttta ctttcgggcc gtaaaccagt accggaatgt gttcacgcgt
     1021 gtggtcagta ccggtccagg tcggatcaca accgtggtca gcagtgagga tcaggatgtc
     1081 gtcatcgcgc agcagagaca tcagctccgg cagacggcgg tcgaacagct ccagacccac
     1141 agcataaccg gcgacgtcgc gacggtggcc ccaggaagag tcgaagtcaa cgaagttggt
     1201 gaagacgatg gtgttatcac ccgcttcttt catctctttg atggtggcgt caaacagcgc
     1261 gtccaggcca gttgctttca ctttcttggt gataccgcag ttggcgtaga tgtctgcaat
     1321 tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc agtttctgca gcacggtcgg
     1381 tgctggcggc tcaacagcca ggtcgtgacg gttaccggta cgctggaagt taccggcttt
     1441 gtcaccgata aacggacgag cgataacacg accgatgttg tagccgccgt tggtcagctc
     1501 ttcacgggcg atttcgcaca gttcgtagag tttatccagg ccaaaggttt cttcgtggca
     1561 ggcaatctgg aacacagagt cagcggaggt atagaaaatc ggcttgccgg ttttcatgtg
     1621 ctcttcgccc agctgatcca gaatgaccgt accggaagag tggcagttac cgaggtaacc
     1681 cggcagatta gcgcgttcaa ccagtttatc cagcagctct tgcgggaagc tgttttcgtg
     1741 atcggagaaa tatccccact caaatagaac cgggacaccg gctatttccc agtgaccaga
     1801 cggggtatct ttaccggatg acatttcgtg cgcccatgcg tacgcgccga taacttcagc
     1861 gttgccgtcc attcccgccg gaatgaaacc ggtagaacct tcgtgtgctt tcgccagccc
     1921 cagacgggtc agatttggca gattgagcgg acctttacga ccgttatcag cttcgccttt
     1981 ggcacaagct tctgcgatat gacccagggt gtcagccccg acgtcaccaa agcgttctgc
     2041 atcttctgta gcgccgatgc cgaatgagtc cagcaccata ataaatgcac gtttcatatg
     2101 ttctccgtac tcagtgcttc aaatttaaat gatcagatca gtataccgtt attcgctgat
     2161 acggcgatag acagttggtg tgctttccgg tgctttatcg gcaagtttaa ttgccgcttt
     2221 caccgctttc gccgcttcct gccagctgtt ttcgtctttc gcgtggataa ccgccagcgg
     2281 acgctgaccg tctacctggt cgcccaaacg cgccatatca gtaaagccga cgctgtaatc
     2341 aatggtgtca gatgcctgac gacgaccgcc gcccattgca accactgcca tccccagcgc
     2401 gcgggtatcc atttcactga caaaaccttc ggtatcagca tagactgctt tcgtcagcat
     2461 cgctgtcggc agatacttcg cgtagttctc aacgaagtcg gttgggcctt tttgtgccgc
     2521 taccatacga ccaaagactt ctgccgcttt accgttgtcc agcactgctt gcaatttcgc
     2581 gcgcgcttcg gcgtcatctt tcgccagttt gccggagata agcatctcca cgcacagcgc
     2641 catcgtgaca tcaaacagac gcgggttacg atactcaccc gtcaggaact gcaccgcttc
     2701 acgaacttca accgcgttac ctgcactgga ggccagtacc tgattcatgt cggtgagcag
     2761 cgcggtggtg cgcacgccag cgccgttagc cacgccaaca atcgcttcgg caagggcttc
     2821 agagagttcg taggtcggca taaacgcgcc gctacccact ttcacgtcca tcaccagcgc
     2881 gtccagacct tccgcaagtt tcttcgccag aatagaggcg gtgatcagcg ggatggagtc
     2941 cacggttgcg gtaatatcac gggtcgcgta gaaacgttta tccgccggag ccagtgagct
     3001 ggtctgaccg ataatcgcca cgccgacgtc tttaataatt tcgcggaaac ggttgtcatc
     3061 cgggaaaatg tcgaagccag ggatggattc cagtttgtcg agcgtaccgc cagtatgacc
     3121 gaggccgcga ccagagatca tcggaatata gccgccgcag gctgcgacca tcggccccaa
     3181 catcagcgaa gtcacatcgc cgacgccacc agtggaatgt ttatcaacaa tcgggccatt
     3241 cagatgcagg cttttccagt cgagaacggt tcctgaatct cgcatcgcca tggtcagcga
     3301 gacacgctca ggcattgtca tatcgtggaa gaaaatggtc atcgcgaggg cggcaatctg
     3361 cccttcggag atagtgttgt cgcgaatgcc gttgataaag aaacgaattt cttcatcgct
     3421 cagtgcatga ccatcacgtt ttttacgaat aatttcttgt gcgagaaaca aggtaacctc
     3481 ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc tacgccttat ccggcctacg
     3541 ggtagatacg tctcgtaggc ctgataagcg cagcgcatca ggcgtaaagc atcttactta
     3601 gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct ttcaacaggc ttgccagcag
     3661 gctggaagca ccaaagcggt agtgacgcgc atctgcccag tcagcaccga acagttcatc
     3721 tgcgatggcg agatatttct gcgcatcttc cgcagtacgc acgccgcccg ctggtttgaa
     3781 accaacggtt ttttctacgc ccatatcacg gatcacttcc atcatgatgc gcgcgctttc
     3841 cggcgtcgcg ttcacagcca ctttaccggt agaggttttg atgaagtccg cacccgcttt
     3901 gatggagatt tcagacgctt tacggatcag cgcttcgtct ttcagttcgc cggtttcgat
     3961 gatcactttc agcagtacat tcgctgccgc gcaagcctct ttacaggctt tcaccaggtc
     4021 aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac gggaacacaa cgtcaacttc
     4081 atcagcaccg taggcgattg ccgcacgggt ttctgccagc gcgatttcga tgtcatcgtt
     4141 accgtgtggg aagttggtta ccgtagcgat tcggatttcc ggggtgccct gctctttcag
     4201 cgttttgcga gcaatcggga taaagcgagg atagatacag atagcggcgg tattgccgac
     4261 cggggttttg gcctgatgac acagagcaat tactttctcg tcggtgtcgt cgtcattcag
     4321 ggtggtcagg tccatcaatt tcagtgcacg caggctgctt gctttcagat cagtcatttc
     4381 attctcctgg cttgtcgcca ataaaattca ccttgcgagt ttgttagtat tctaacatct
     4441 actccgcaac acacttcgat atacatcaca attaaggaaa tctacttaca agtttgcatc
     4501 actgtaatgc gatctggttc aaataattca ctttcaaatg aatgcgtcag tggtggcaaa
     4561 cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa tggcgcatca ccttcggcat
     4621 aaaaggaaaa gaagatgccc acttctcatg aaaatgcact gcaacaacgt tgccagcaaa
     4681 ttgtcaccag cccagtgctt agcccggagc agaagcgcca ttttctggca ctggaagcag
     4741 aaaacaatct gccttatccg cagcttcctg ccgaagcccg ccgcgcgctg gatgagggtg
     4801 taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc gcgctatgtc ttacccgatt
     4861 acgcccgttt tctggcgaac ggttccgaat ggctggagct ggaaggcgcg aaagatcttg
     4921 atgacgcact ctctctgctg accattcttt accaccacgt accgtcggtc acatcgatgc
     4981 cggtctacct ggggcaactg gatgcgttgt tgcaaccata tgttagaatt ctaacacaag
     5041 acgagatcga tgttcgaata aaacgtttct ggcgttacct cgacagaacc ctgccagacg
     5101 cctttatgca cgccaatatc ggcccgtctg attcgcccat tacccgcgcg atcttacgtg
     5161 cagacgcaga gttgaagcag gtttcaccga acctgacctt tatctacgat cctgaaatca
     5221 cccctgatga cctgctgctg gaagtggcga agaacatctg tgaatgtagc aaaccgcaca
     5281 tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gggctacggg attgtgagct
     5341 gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt aacctgaaag
     5401 ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta ccgcactact
     5461 gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa caatcacact
     5521 tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt tttgtgccaa
     5581 tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtaagaaa gaagggattg
     5641 ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg ttatcgcatc agcgcgcaac
     5701 tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc gccatgttac
     5761 acgcacagtc ggggagcagt tccgatatcg gcaccacgcc gggcgcgcgt ttgccgtatg
     5821 gtgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat gcttattatt
     5881 attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaat ccgcaggcgc
     5941 tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttact gccaatgtca
     6001 gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat ttagaaaaat
     6061 atcgcgccga aggttcacgc accaacacca cctggctggg cgaagaagcc gcacgcaaca
     6121 ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg cgctttagtc
     6181 agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc tctgtttttg
     6241 cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg acgttgcaat
     6301 gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt tgacggcaaa
     6361 gtggtgtgga acgctgtggt ttgcgagcag tgtgatacct gcctgaagag gtgtccgcaa
     6421 catgccacgc caatggcgca atccatgagc gtggacgaag tgcttagcca tgtccgcaaa
     6481 gcggtgctgt ttattgaagg gataacggta agcggcggcg aagccacgac ccaactgccg
     6541 tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca cctcacctgc
     6601 ctggtggaca gtaacggcat gttgagcgaa accggctggg aaaaattact cccggtgtgt
     6661 gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat gtcatcaaca cttaaccgga
     6721 cgcgataatc agcagattaa gcgcagcatc tgtttgcttg cagagcgcgg caagctggcg
     6781 gaactgcgtt tgctggtaat tccagaccag gtggattatt tgcatcacat cgatgagctg
     6841 gcgacgttta tcaagagact tggcgatgtt ccggttcgcc tgaatgcgtt tcatgcccac
     6901 ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt tgagccgttg
     6961 gctgatgcgt taaaggtgcg cggggtgagc cggttgatat ttccggcgct ctatttgtga
     7021 catgccggat acggcctacg gttcggcaca gacttgtagg catgataaga cgcgacaagc
     7081 gtcgcatcag gcattatgcg ccgactgccg gatgcggcgt gaacgcctta tccggcctac
     7141 ggttcggcac agacttgtag gcatgataag acgcgtcagc gtcgcatcag gcattgtgcg
     7201 ccaactgccg gatgcggcgt gaacgcctta tccggcctac ggttcggcac agacttgtag
     7261 gcatgataag acgcgtcaag cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg
     7321 tgaacgcctt atccggccta cggttcggca caaaccagta ggcatgataa gacgcgtcaa
     7381 gcgtcgcatc aggcattgtg cgccaactgc cggatgcggc gtgaacgcct tatccggcct
     7441 acggttcggc acaaacctgt aggcatgata agacgcgtta agcgtcgcat caggcattat
     7501 gcgccgactg ccggatgctg cctacgaagg gtaggctacc cactaacgct aaacaacgca
     7561 tacgtattat taagcaacac ttcggcaatc tcatcttctg gttccgggcg caactcgcaa
     7621 agcacgtcaa acacccgcac agcctgttcc ggtcggttag gctgcccctg aaagccgttg
     7681 agcggcatat ccggcgcgtc ggtttccagc aacaacgatg ccagcggtaa ttttgcgatg
     7741 acatcgcggg ttttactggc gcgtggatag gtgatagtac cgcctacgcc gattttgtag
     7801 cccagctgta caaaccgctc ggcctgttgc aggctgccgg aaaaaccgtg aaccacgcca
     7861 gtgcacggta aatcgtgacg tttaagatgc atcgccagtt tgtcgtgtat acgccgggaa
     7921 tgaaggatca ccggcagatc gtagcgtttc gccagtttca gctgttcgtc gagtaaccac
     7981 tgctgcctct caaattgcgg atcgtcgccg aagagatcca gaccgatctc ccccaccgcc
     8041 accaccttcg ccggacgcct ttccagcgcc tgctgtagct gatcaagaga cacatcgcta
     8101 tgtttttcca acatccccgg atgcaacccc aatgcggcat acagcggttg atatttttcc
     8161 gctaacgcct gcacgcgggc aaaattttcc gcctctgtcg ccggaacaat gatcttgcct
     8221 acgcccgctt gtgccgcgcg ttgcaggctg gcctcttcat cgccactaaa cggcgggaaa
     8281 tcaaaatggc agtgggtatc gataaaacgg caaatcaagc cagatcctca ttgttaaatg
     8341 tcgggtcgtt cgcctgcgga gcatcgctca cttcggcaac cagcgtatcg ttggcgactg
     8401 gcgcaggcgg aataacgatc gattccggcg tcaccactgg caccagatgg cgggtaagcg
     8461 gcgctttttc agttaatagc ttgccaaccg tggcgaggaa ataacgaccg cataaacgcc
     8521 caagtttata gtcttcacgc agcgccggaa tccgactgcc aagcgcgata ctatgtaatg
     8581 gcttcggcgg ataaatttcg aatatccgca gcttgcccgg tggtttctca ataaattgct
     8641 gaatgtcacg atagctggtt tcatgatgct gcaccagatt gaccagcggc tgcaggctac
     8701 tgtcacccag ccagcgttcc atgcgtttga accactgcgg cgtgtagtac atttgtgacg
     8761 gcacagtgcg aatgacgacc aacgttttag cgccctgcct tgccgcctct ttaaccggaa
     8821 tcgcatcact gatcccgcca tccaggtagt taatgccttc cagcgacact ccgctacgat
     8881 aaaagccagg tatcgcactg gaggcgcgaa tcacatccag ccagttttgt ttggttggta
     8941 aaaagtaatt aggcgcgtag tcatcctgac gacaggcgca catataaaac gatttgccgc
     9001 tgtcaaacaa ccgcgcggcg gtatccattt gcaacggcat ctggcttgcg gtggcctcca
     9061 ccagccagtc gagatcgata agatttcctc cacggacaaa gcgcaatgga tcgaaaaatt
     9121 cgcgttttgt ggtatagcgc atgatgactt tgcgcgcgta accgggctga ttgcagataa
     9181 acgccgagag gttctgcgcc ccggcagatg tgccgagata aagatcgaaa ggattaaact
     9241 gcgcgcgcat aaactcatcc agcacgccag ccgtgaaaat tccacgctgt ccgccgcctt
     9301 cgcacaccag agctattcgt ccaggctgga acggccttag cgacaacggc gcaatattac
     9361 caagcgttac aggtattcgc tgccccacct ctgccttcct gtttttgttg tgaaagaatg
     9421 acaaagtaac gcaatttatt attcgctaaa accgcaaaag ccagtccgct ggactggctt
     9481 tggataaaat tttcttaatg gtctgaaatc tagggtcgtt ttcggcccat gaacaaactc
     9541 accaggaaca gaataatccc gacgacaaag acaattttag ctgcgcctgc agcggtaccg
     9601 gccagaccac caaacccaag tgcggcggcg attaacgcga taaccagaaa tatgatgccc
     9661 caacgaaaca taagcgtctc ctttaccata gttaatgtca ccgcttgata tgagcgtgaa
     9721 aatcactcaa tgcgatattt ttagtgtggt gcacatcgcg cctcccgaca aagttcggga
     9781 ggacgaatta cgacgaatta cttagttttc agatcatttt taacgctttt cacaccatct
     9841 accgctttgg cgatactttc agcacggtcg ctctgtgcct gggaatcgac ggtaccggag
     9901 agctgaacca cgccgtcggt ggtttcaact ttcacatgac gggaagggac gatatcgtcc
     9961 gccagcagtt tggctttgat ttcactggtg gtggcggtgt cacccgcgta gcccttcaca
    10021 gagccttctt tagcgtcgcg aacgtgcagt ttgtcgctga cagaggtcac cccttcaacg
    10081 cctttcgcca ctttcactgc ctcttcggcc tgagcctggc tttcaacgaa accgctcagg
    10141 gtcacgactt tttgatcggt tttgacagag atatcggtgc tcttgatgtt gtcatgatcc
    10201 accagcgccg ccttcacttt cgcggtgatg gcgctgtcat ccatgaaatt accgacttta
    10261 ttcatagagc tatcgacttt ttgccctgcg ctttcattgg tagtctgtgc gttgttttcc
    10321 gcgtaggcag agccggtcgc gacggcagag gtcaacatta cagccagcag agttttcgaa
    10381 atcttcagtc ttgtcatagt catcgattta ttcctgtatg tttgctcgta atttgagcct
    10441 ggcaacacga ggttgcattg ctgaataggg agagacttca ccctctacag aagtcaatgg
    10501 tcgccatcac aaaagcgatg ggtgatgaat aacgaccatt acagcctctg aatcagttat
    10561 taatatcggt agaatgacaa tcgacggctt tagatactga tatctacgca ttgaacggta
    10621 tttaacgccg tcagaaatgt cattactttg ttaaatatag atcacaattt tgaaaccgct
    10681 cgggatatca gcgaaaacat aagcaaaagt gaatgtttta agaacattcc gtaagcggct
    10741 aataaggaag ggaaattgac agggcgcagc ggctaccgcg ccctggaggc aagaattaat
    10801 gctcgcgggt ctggtggaac tgaacgtcgg gataacgttc ctgcgccagg cgcaggttga
    10861 ccatgctggt agcgatgtaa gcgaggttat cgccgccatc aagcgccagt tggctttcgt
    10921 tcttacgctt gaactcttcg aatttcttcg cgtccgcaca ttctacccag cgggcagtgg
    10981 caacgttgac tgattcatat actgcttcaa cgctgtattc gctcttcagg cgcgatacca
    11041 ccacatcaaa ctgcagcaca ccaacagcac caacgatcaa atcgttgttg gagatcggac
    11101 ggaacacctg caccgcgccc tcttcggaaa gctgtaccag ccctttgagc agctgttttt
    11161 gcttcagcgg atctttcagg cggatacgac ggaacagttc tggtgcgaag ttcggaatac
    11221 cggtgaactt catcatctca ccctgggtaa aggtgtcgcc gatctgaata gtgccgtggt
    11281 tgtgcaggcc gaggatatcg cccggatacg cttcttcaac gtgcgaacgg tcgcccgcca
    11341 taaaggtcag cgcgtcggag ataaccacat ctttcgcagt acgcacctgg cgcagcttca
    11401 tgcctttttc atatttaccg gatactacgc gcataaacgc cacgcggtcg cggtgtttcg
    11461 ggtccatgtt ggcctgaatt ttaaatacga agccggtaaa tttgtcttcg ctcgcctcta
    11521 cggtacgggt atcagtctga cgcggcatcg gcgcaggagc ccactccacc aggccatcca
    11581 gcatatgatc aacgccgaag ttacccagcg cagtaccgaa gaataccgga gtgatttcgc
    11641 ccgcaaggaa caattctttg tcgaactcgt tagacgcgcc tttcaccagt tccagttcgt
    11701 cacgtagctg ctgtgccaga tcttcaccaa ccgcagcgtc gagatccggg ttattcagcc
    11761 ctttaacaat acggacttcc tgaatcgtgt ggcctttacc gctctgatag agataggttt
    11821 cgtctttata aaggtggtaa acgcctttaa acagcttgcc gcagccaatc ggccaggtga
    11881 tcggtgcgca gccgattttc agctcgttct caacttcatc gagcagttcc atcgggtcgc
    11941 ggatatcacg gtcaagtttg ttcataaagg tgaggatcgg cgtgtcgcgc agacgggtaa
    12001 cttccatcag cttacgggta cgatcttcaa caccttttgc agcgtcgata accatcaggc
    12061 agcagtccac cgccgtcagg gtacgatagg tatcttccga gaagtcttcg tgccccgggg
    12121 tgtcgagcag gttaaccagg caatcgtgat acggaaactg catcacagac gtagtaatgg
    12181 agatcccacg ctgcttttcc atctccatcc agtccgactt agcgtgctgg ttggaaccac
    12241 ggccttttac tgtaccggcg gtctgaatgg cctgtccgaa cagcagcacc ttctcggtga
    12301 tggtagtctt accggcatcc gggtgagaaa taatggcaaa agtgcggcgc ttcgccacct
    12361 cttgcaaata aggagacaac gtcataattc tttcttcttg agtaagcggc atcgcgccgc
    12421 gcttgttgaa aacgaaaaat tgcggctatt ttacccatca acggggggga ggcaatcagt
    12481 gtttacacag gagctgctcc agttcgtgca acgaagaaac ggtccaggtg ggcgcgatgc
    12541 cttctggttg ctcgcgattg tgcgcattca gccagcaggt cgcaagcccg gcgttgatgc
    12601 caccgagaat atcggactcg gcagtgtcgc caaccatcag cacgcgtgaa cggtcaggat
    12661 tgcccgcctg ttccagcgca taatcgaaaa ttttcttatt cggtttggca acgccaactt
    12721 cttcggaaat caccagcaaa tcgaagtaat cacgcaggcc cgtgcgttcc agacgcactt
    12781 gctgcaaggc gctaaagccg ttggtgatga tgccgatttt ggcgttgcca cgaatggcgt
    12841 taagcagaga aaccgcgccc ggcagcggcg tgcagatttc cgccatcgca ttaataaagg
    12901 catcgttaag caagcctgct tcaactttca gccgttcggc ccagctctca aaacgcccgt
    12961 gctgaagctg taatgaagtg atcgcgccgt tttgataatc cacccacagt ggcttgttaa
    13021 cggcctgata gtcctgaaaa tcttcagcgg taaaggtgac gctgtaatca agaaacatcc
    13081 gctgcaggcc ggtgaatgag tcaaaggtaa acagcgtttc atcggcatca aagaaaatcc
    13141 agtcccactt cattattcca ccttgtctta catactgatt ggcaacgcca tgatgatggc
    13201 gtcttcgcga ccgtccgtgg tggggtagta attgcggcga atcgtcgcct cgttaaagcc
    13261 taaactttcg tacagggcaa tggcggcagc atttgaggca cggacttcca gccatagtgt
    13321 cgccacgccg cgtttttcca gttcgtcgat cagatgttcc agcagcaccc ttcccaatcc
    13381 ctgacgctga tagtcaggat cgaccgcaat attgaacaat gtagcttcat ccagcaccac
    13441 ttgcgtaatc gcaaacgccg ccattttgcc gttttgcgtt aactgaaagt tgagataacg
    13501 ctcgccctgg ttgctggcaa aggttttttc actccacgga aaggcgtggg cgcgttgttc
    13561 aatgtggtaa gccgctggta aatcagtcgt ttcgagggaa gaaatcgtgt tcatatgtgc
    13621 aaatctgttg ccataacgcg gcgcgtgccg ttgggtttgc ccgtaattcg gtgagcgccg
    13681 gtgatgccac ctgagcgcct tccagtgata gcggttcgtc agtacccaac cgccagctgt
    13741 tgcagcgact gccttgcggc agcatcgcga ttttttctgg cgtcagttgc agcacttgat
    13801 cggggctgac ggttaatgcg cgcagaacat cgctcactaa aggatcggtc agggcgggaa
    13861 gatcgtttgc caccatcacc agacggacgt gtgccgggat cgcaatggca atctccccct
    13921 gcaacgcgcc agggcgacgc agcgaccact gggtaatgcc cagttgctgt aactgccagt
    13981 ctcgtcggga tgtcatagcg aatcgctcct gttgtcaggg gcgcaaatat agcaaattcg
    14041 tcgataccgc gccaacatat ggctataatc gccgccagta tcaattgagg agcattccat
    14101 gtctgcattt accccggcaa gtgaagtctt gctgcgtcac agtgatgatt tcgaacaaag
    14161 ccgtattctg tttgccggag acttacagga tgacctgccc gcgcgtttag ataccgcggc
    14221 cagccgtgct catacccagc aattccacca ctggcaggta ttaagccgcc agatggggga
    14281 taacgcccgt ttcagtctgg tcgccacggt ggatgacgtc gcagattgcg atatgctgat
    14341 ttactactgg ccgaagaaca aaccggaagc ccagttccag ttgatgaatt tactttctct
    14401 gctgccagtg gggacagata tttttgtcgt tggcgagaac cgcagcggcg tgcgcagcgc
    14461 cgagcagatg ctggcagatt atgcgccgtt gaataaagtc gacagcgctc gtcgctgtgg
    14521 cctctatttt ggtcgtctgg aaaaacagcc ggtatttgat gccgataaat tctggggcga
    14581 atacagcgtc gatggcctga cggtcaaaac gctgcctggc gtgtttagcc gcgacggtct
    14641 ggatgtcggt agccagttgc tgctctcgac gttaactccg cacacgaaag gtaaagtgct
    14701 ggatgtcggc tgtggcgcgg gggtgctttc agttgccttt gcgcgccatt cgccgaaaat
    14761 tcgtctcacc ttgtgcgatg tctctgcgcc agcggtagaa gccagccgcg caacacttgc
    14821 ggccaactgt gttgaaggtg aagtctttgc cagcaacgtc ttttccgagg tgaaaggtcg
    14881 ttttgatatg atcatctcca acccgccgtt ccacgatggg atgcaaacca gcctggatgc
    14941 ggcgcaaacg ctgattcgcg gcgcggtgcg tcatcttaat agcggcggcg agctgcgaat
    15001 tgtagcgaac gccttcctgc cttacccgga cgtgctggat gagacatttg gcttccatga
    15061 agtgattgcg caaaccgggc gtttcaaggt gtatcgcgcc attatgaccc gccaggcgaa
    15121 gaaaggttaa ttatctcgcc gaataccgtg tcggatgcgg cgtgaacgcc ttatccgacc
    15181 tacaaaacct gcacgttagc ccttcgtagg ccagataaga cgcgccagcg tcgcatctgg
    15241 catctgcccc cgcatgccta ccaaacctgc acgctagttt cctgatggac atttttccag
    15301 caattacacc tccggcgata attaactatt gacgaaaagc tgaaaaccac tagaatgcgc
    15361 ctccgtggta gcaattcttt ttaagaattg atggtatgcg aaggtggcgg aattggtaga
    15421 cgcgctagct tcaggtgtta gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc
    15481 accaaaaacc acgttgatat tgctcgcact gggcgaaggt ggcggaattg gtagacgcgc
    15541 tagcttcagg tgttagtgtt cttacggacg tgggggttca agtctccccc ctcgcaccaa
    15601 acgaggcgat atcaaaaaaa gtaagatgac tgtgcgaagg tggcggaatt ggtagacgcg
    15661 ctagcttcag gtgttagtgt ccttacggac gtgggggttc aag
//
LOCUS       DAZOXR010000089        15659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZOXR010000089 DAZOXR010000000
VERSION     DAZOXR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.091"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            130..465
                     /locus_tag="ACHUNN_002468"
     CDS             130..465
                     /locus_tag="ACHUNN_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0576819.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            623..1066
                     /gene="yjdN"
                     /locus_tag="ACHUNN_002469"
                     /pseudo
     CDS             623..1066
                     /gene="yjdN"
                     /locus_tag="ACHUNN_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            1199..1987
                     /gene="phnC"
                     /locus_tag="ACHUNN_002470"
     CDS             1199..1987
                     /gene="phnC"
                     /locus_tag="ACHUNN_002470"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0576820.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2012..3028
                     /gene="phnD"
                     /locus_tag="ACHUNN_002471"
     CDS             2012..3028
                     /gene="phnD"
                     /locus_tag="ACHUNN_002471"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0576821.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3154..3933
                     /gene="phnE"
                     /locus_tag="ACHUNN_002472"
     CDS             3154..3933
                     /gene="phnE"
                     /locus_tag="ACHUNN_002472"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR0576822.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3954..4679
                     /gene="phnF"
                     /locus_tag="ACHUNN_002473"
     CDS             3954..4679
                     /gene="phnF"
                     /locus_tag="ACHUNN_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR0576823.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4680..5132
                     /gene="phnG"
                     /locus_tag="ACHUNN_002474"
     CDS             4680..5132
                     /gene="phnG"
                     /locus_tag="ACHUNN_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR0576824.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5129..5713
                     /gene="phnH"
                     /locus_tag="ACHUNN_002475"
     CDS             5129..5713
                     /gene="phnH"
                     /locus_tag="ACHUNN_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR0576825.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5713..6777
                     /gene="phnI"
                     /locus_tag="ACHUNN_002476"
     CDS             5713..6777
                     /gene="phnI"
                     /locus_tag="ACHUNN_002476"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR0576826.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6770..7615
                     /gene="phnJ"
                     /locus_tag="ACHUNN_002477"
     CDS             6770..7615
                     /gene="phnJ"
                     /locus_tag="ACHUNN_002477"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR0576827.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7612..8370
                     /gene="phnK"
                     /locus_tag="ACHUNN_002478"
     CDS             7612..8370
                     /gene="phnK"
                     /locus_tag="ACHUNN_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR0576828.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8481..9161
                     /gene="phnL"
                     /locus_tag="ACHUNN_002479"
     CDS             8481..9161
                     /gene="phnL"
                     /locus_tag="ACHUNN_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR0576829.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            9158..10294
                     /gene="phnM"
                     /locus_tag="ACHUNN_002480"
     CDS             9158..10294
                     /gene="phnM"
                     /locus_tag="ACHUNN_002480"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR0576830.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10294..10851
                     /gene="phnN"
                     /locus_tag="ACHUNN_002481"
     CDS             10294..10851
                     /gene="phnN"
                     /locus_tag="ACHUNN_002481"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR0576831.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10838..11272
                     /gene="phnO"
                     /locus_tag="ACHUNN_002482"
     CDS             10838..11272
                     /gene="phnO"
                     /locus_tag="ACHUNN_002482"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR0576832.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11274..12032
                     /gene="phnP"
                     /locus_tag="ACHUNN_002483"
     CDS             11274..12032
                     /gene="phnP"
                     /locus_tag="ACHUNN_002483"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR0576833.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12179..12508)
                     /gene="yjdP"
                     /locus_tag="ACHUNN_002484"
     CDS             complement(12179..12508)
                     /gene="yjdP"
                     /locus_tag="ACHUNN_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR0576834.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12577..12894)
                     /locus_tag="ACHUNN_002485"
     CDS             complement(12577..12894)
                     /locus_tag="ACHUNN_002485"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR0576835.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13147..15132
                     /gene="yjcS"
                     /locus_tag="ACHUNN_002486"
     CDS             13147..15132
                     /gene="yjcS"
                     /locus_tag="ACHUNN_002486"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR0576836.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            15341..15616
                     /locus_tag="ACHUNN_002487"
     CDS             15341..15616
                     /locus_tag="ACHUNN_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR0576837.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         3556 a         4590 c         4286 g         3227 t
ORIGIN      
        1 gaaaacgatc gtcgtctaaa atcagcagta cccccgacaa actcagggat tttgtgtata
       61 attgcggcct ttttcggcaa tctgccgttt tttggcgctt ttgccctgct gacttttgag
      121 gaaatccaca tgtcattacc acactgccca aaatgcaact ccgaatacac ttacgaagat
      181 aacggcatgt acatctgccc ggaatgtgcc tacgaatgga acgacgcaga acctgcacag
      241 gaaagcgacg agctgatcgt taaagatgct aacggcaatc tgctggctga cggcgacagc
      301 gttaccatca ttaaagatct gaaggtgaaa ggtagctctt cgatgctgaa aattggcacc
      361 aaagtgaaaa acatccgcct ggttgaaggc gaccataaca tcgattgcaa aatcgacggt
      421 tttggtccga tgaaactgaa atctgagttt gtgaaaaaga actgattgta ttgtgatcgg
      481 taagccggat aaggcgctcg cgccgcatcc ggcaacgatg ccgactgcct gatgcgacgc
      541 ttgcgcgtct tatcaggcag cctctcatct gtataaattt cgaactacac ttaattggct
      601 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      661 tgccgacgca attgcttatt atcaacgtac gttgggcgct gaactgctct ataaaatcag
      721 cttcggcgaa atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca
      781 atttcccgat accgccatcg cccacgccaa tgtgcgcatt gccggaagcg acatcatgat
      841 gagcgatgcc atgccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc
      901 gcaacaggtc gaagaaggaa aacgctggtt tgacaatctt gccgctaacg gaaaaatcga
      961 aatggcctgg caggaaactt tctaggcgca tggctttggc aaagtcaccg ataaatttgg
     1021 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataacccg ccgggaggcc
     1081 cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt
     1141 aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1201 gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aggcgctgca
     1261 tgcggttgct ctgaacattc atcacggtga aatggtggct ctgcttgggc cgtcgggttc
     1321 cggcaaatcc acccttttac gtcacttaag cggtttgatt accggcgata aatccgccgg
     1381 cagccatatc gagctgctgg gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat
     1441 ccgcaaaagc cgcgccaaca ccggctacat cttccaacaa ttcaacctgg tgaaccgcct
     1501 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg
     1561 ttttagctgg tttacccgcg agcagaaaca acgcgcgtta caggcgctga cccgcgttgg
     1621 catggtgcat tttgcccatc aacgcgtttc caccctctcc ggcggacagc agcagcgtgt
     1681 ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc
     1741 ctcgctggac ccggaatccg cccgcatcgt gatggacacc ctgcgcgaca tcaatcagaa
     1801 cgacggcatc accgtggtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     1861 acgcatcgtc gccctgcgcc aggggcacgt tttctacgac ggcagcagcc aacagtttga
     1921 taacgaacgt tttgaccatc tctaccgcag cattaatcgc atcgaagaga acgcgaaagc
     1981 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2041 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga
     2101 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaatg
     2161 gacgccattc ttacaggata tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc
     2221 cccagactac gcaggcatta tccagggaat gcgcttcaat aaagtggata tcgcctggta
     2281 cggcaacctg tcggcaatgg aagcggtgga tcgcgccaac ggccaggtct tcgcccagac
     2341 ggtcgcggcg gatggatcgc caggttactg gagcgtgttg atcgtcaaca aagatagtcc
     2401 gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga
     2461 tcctaactcc acctctggct tcctcgtccc cggttactac gtctttgcca aaaacaatat
     2521 ctccgccagc gactttaagc gcaccgtcaa cgccgggcat gaaaccaacg cgctggccgt
     2581 cgccaacaag caggtggatg ttgccaccaa caacaccgaa aacctcgaca agctgaaaac
     2641 ctccgcgcca gagaagctga aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg
     2701 cgatccgatc gtctggcgca agaatctctc cgaaaccacc aaagacaaga tctacgactt
     2761 ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc
     2821 accgtttcgc gcctccagcg acctgcaact ggtgccgatt cgccagctcg cactgtttaa
     2881 agagattcag ggcgtaaaaa gcaataacgg actgaatgag caggacaagc tggcgaaaac
     2941 caccgagatt caggcgcagc tggatgacct ggaccgcctg aacaacgcgt taagcgcgat
     3001 gagttcggtg agtaaagcgg tgcagtaatg tgctcgatcg gtcccctctc ccttgaggga
     3061 gagggttagg gtgaggggga aatgcgcacg ctgatgccct caccccggcc ctctcccaca
     3121 gggagaggga gaaaacatta aacggagcca aacatgcaaa ccatcaccat cgccccaccc
     3181 aagcgcagct ggttctcgct tctgagctgg gccattgttc tcgccgtgtt ggtcgtctcg
     3241 tggcagggcg cggaaatggc cccgcttacg ctgatcaaag acggcggcaa catggcgacc
     3301 ttcgccgccg acttcttccc gcccgatttc agccagtggc aggattacct caccgaaatg
     3361 gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cagtggttct ctccatcccc
     3421 ttcggcctga tgagcgccga aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc
     3481 ctgatggacg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc
     3541 gtcggtctcg gaccgttcgc tggcgtgctg gcgctattta ttcacaccac cggcgtgctc
     3601 tcgaaactgc tttccgaagc ggtggaagcg attgaacccg gcccggtgga aggcattcgc
     3661 gccaccggcg ccaacaagct ggaagagatc ctttacggcg tgctgccaca ggtaatgccg
     3721 ctgctgatct cctactccct ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc
     3781 ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg aagcgattcg cggtttccag
     3841 ttccaacaaa cctgcgccct gatggtgctt atcatcgtca cggtcagcct gctggatttc
     3901 ctctctcaac ggttgcgtaa gcactttatc tgataagtga ggcattgata tctatgcact
     3961 tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca aaacttgagc
     4021 aggagcttcg tcaacactat cgctgcggcg actatcttcc cgccgagcag caactggcgg
     4081 cgcgctttga ggtaaaccgc cacaccctgc gccgcgccat cgaccaactg gtggaaaaag
     4141 gctgggtaca gcgccgtcag ggcatcggcg tgctggtgct gatgcgcccg ttcgattacc
     4201 cgctcaacgc ccaggcgcgt tttagccaga acctgctgga ccagggcagc catcccacca
     4261 gcgaaaaact gctgtcggta ttgcgccccg cgtccggtca cgttgctgac gcgctgggga
     4321 ttaccgaggg tgagaacgtt atccatttgc gcaccctgcg tcgcgtcaac ggcgtcgcgc
     4381 tctgtttaat cgaccactac tttgcggacc tcaccctctg gccgacactg caacgtttcg
     4441 acagcggttc gctgcacgat tttctgcgcg agcaaaccgg aattgcgctg cgccgcagcc
     4501 agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg ccagcgcctt gaaatcccga
     4561 atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg
     4621 cggagtactc cgtcagcctg acgcgcgccg acatgattga attcactatg gagcactgaa
     4681 tgcacgcaga taccgcgacc cgccagcact ggatgtccgt actggcgcac agccaaccgg
     4741 ctgaactggc agcccgcctg aacgcgctga acatcaccgc cgactatgaa gtgatccgca
     4801 ctgccgaaac tggcctggtg cagattcagg cgcgaatggg cggtaccggc gaacgctttt
     4861 ttgccggcga cgccacgctg acccgtgccg ccgtacgcct gactgacggc accctcggct
     4921 acagctgggt gctggggcgt gataaacagc acgccgaacg ctgcgcgctg attgatgcgc
     4981 tgatgcagca atctcgccac tttcaaaact tatcagaaac ccttattgcc ccgctggacg
     5041 ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt gaacgccagc cgggtcgact
     5101 tctttacgat ggttcgcgga gacaacgcat gaccctggaa accgctttta tgcttcccgt
     5161 gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc atgagcgagc cgggcgtgat
     5221 tgtcgccctg tatcagctca aacgcggctg gcagccgctg aatatcgcca ccaccagcgt
     5281 gctgctgacg ctggccgata acgacacgcc ggtgtggctt gccacgccat taagtaacga
     5341 tatcgtcaat cagagcctgc gttttcatac caacgcgccg ctggtcagcc agccggaaca
     5401 ggcgaccttc gcggtgacgg atgaggcgat ttccagcgaa cagctcaacg ccctttccac
     5461 cgacaccgcc gttgcgccgg aagcgggcgc gacgctgatt ttacaggtcg ccagcctgag
     5521 cggcggacgc atgttgcgtc tcaccggcgc gggcattgcc gaagaacgaa tgatcgctcc
     5581 gcagctgcca gagtgcattc tgcacgaact caccgagcgc ccgcacccgt tcccgctcgg
     5641 catcgaccta atcctgacct gcggcgagcg cctgctggct atcccgcgaa ccactcatgt
     5701 ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaaa aggcgatcga cgccgcccac
     5761 gccctgcaag agagccgacg ccggggcgat accgatttgc ccgaactgag cgtcgcccag
     5821 attgaacagc agcttaacct cgcggtggat cgcgtgatga cagaaggcgg cattgccgac
     5881 cgcgaactgg cggcgctggc gctgaaacag gccagcggcg ataacgtcga agcgattttc
     5941 ctgctacgcg cctaccgcac cacgctgacg aaactggcgg taagcgagcc gctcgacacc
     6001 acggagatgc gtctcgaacg gcgtatttca gcggtttata aagatattcc cggcggccag
     6061 ctgcttggcc caacctacga ctacacccat cgcctgctcg atttcactct gctggcaaac
     6121 ggcgaagcgc cgacgctgac caccgccgac agcgaacaac agccgtcgcc gcacgttttc
     6181 agcctgctgg cgcgtcaggg gctggcgaag tttgaagagg atagcggagc acagccggat
     6241 gacatcaccc gcacgccgcc ggtttacccc tgttcacgtt cttcccgttt gcagcagtta
     6301 atgcgcggcg acgaaggcta tttgctggcg ctggcttact ccacccagcg cggttacggg
     6361 cgcaaccacc cgttcgcggg cgagatccgc agtggttaca tcgacgtgtc gattgtgccg
     6421 gaagagctgg gatttgcggt aaacgtcggc gaactgctga tgaccgagtg tgaaatggtc
     6481 aacggtttta tcgacccgcc ggatgagccg ccgcacttca cgcgcggcta cgggctggta
     6541 ttcggcatga gcgagcgcaa agcgatggca atggcgctgg ttgaccgtgc gttgcaagcc
     6601 ccggaatacg gcgagcacgc gacaggcccg gcgcaggatg aagagttcgt gctggcgcat
     6661 gccgacaacg tcgaagccgc aggctttgtc tcgcacctca aactcccgca ctacgtcgat
     6721 ttccaggccg aactggagct actcaaacgt ctgcaacagg agcagaacca tggctaatct
     6781 gagcggctac aactttgcct acctcgacga gcagaccaaa cgcatgatcc gccgcgccat
     6841 cttaaaagcg gtggcgatcc ccggttatca ggtaccgttt ggcgggcgcg agatgccgat
     6901 gccgtacggc tggggaactg gtggtatcca gctcaccgcc agcgtgattg gcgaaagcga
     6961 cgtgttgaag gtgattgacc agggtgcgga tgacaccacc aacgccgtgt cgattcgcaa
     7021 cttctttaag cgcgtgaccg gggtaaacac cactgaatgt acggacgatg cgacgcttat
     7081 ccagacgcgt caccgcatcc ccgaaacgcc gctgaccgaa gatcagatca ttatcttcca
     7141 ggtgccaatc ccggaaccgc tgcgctttat cgagccgcgc gaaacggaga cccgcactat
     7201 gcacgcgctg gaggagtacg gcgtgatgca ggtgaaactg tatgaagata tcgcccgctt
     7261 cggtcatatc gccaccacct atgcttatcc ggtgaaggtg aacgggcgct acgtgatgga
     7321 cccgtcgccg atcccgaaat tcgataaccc gaaaatggac atgatgcccg ccctgcagct
     7381 gttcggcgca gggcgcgaga agcgcatcta tgcggtgccg ccgtttaccc gcgtggaaag
     7441 tctcgatttc gacgatcacc cgttcaccgt tcagcagtgg gatgagccat gcgccatctg
     7501 cggatcgacc cacagctatc ttgatgaagt ggtgctggat gacgccggaa accgcatgtt
     7561 tgtctgctcc gataccgatt attgccgcca acagagcgag gcgaaaagcc aatgaatcaa
     7621 ccgttacttt cggtcaataa cctgacccac ctttacgcgc cgggcaaagg ctttagcgat
     7681 gtctcttttg atttatggcc gggggaagtg ctgggcattg tcggggaatc cggctccggg
     7741 aagaccacgc tgctgaagtc gatctccgcg cgtctgacgc cgcagcaggg ggagattcac
     7801 tatgaaaacc gttccctcta tgcaatgagc gaggccgacc gccgtcgcct gctgcgcacc
     7861 gaatggggcg tggtgcatca gcatccgctc gacggcctgc gccgccaggt gtcggcaggc
     7921 ggcaatatcg gcgagcggct gatggcgacc ggggcgcgac attacggcga tattcgcgct
     7981 accgcgcaga agtggctgga agaggtggag attcccgcca accgtatcga cgacctgccg
     8041 accacctttt ccggcggtat gcagcagcgt ttgcagattg cccgcaacct ggtgacgcat
     8101 ccgaagctgg tgtttatgga tgaaccgacc ggcgggctgg atgtgtcggt gcaggcccgc
     8161 ctgctcgacc tgctgcgcgg actggtggtg gagctgaacc tcgcggtagt gattgtcacc
     8221 cacgatttag gcgtcgcccg cctgctggcg gaccgtttgc tggtgatgaa gcaggggcaa
     8281 gtggtggaga gtgggttaac cgaccgcgtg ctcgacgacc cgcatcatcc gtatacacag
     8341 ctgctggtgt catcggtttt gcagaattga gccggtgccg gatgcggcgt aaacgcctta
     8401 tccggcctac aaatgcgctc cccgtaggtc ggataagacg cgtcagcgtc gcatccgaca
     8461 cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt cagtaaaacc ttcatcctgc
     8521 accagcaaaa cggcgtgcgc ctgcccgtcc tcaaccgcgc ctcgctcatc gtcaacgcgg
     8581 gcgaatgcgt ggtgctccac ggccattccg gcagcggaaa atcaaccctg ctacgctcgc
     8641 tgtacgccaa ctatctgccc gacgaagggc aaatccagat caaacacggt gacgaatggg
     8701 tcgatctggt caccgcgcca gcgcgcaaag tggtggagat ccgcaaaacc accgttggct
     8761 gggtgagcca gtttctgcgc gtcatcccgc gtatttcggc gctggaagtg gtgatgcagc
     8821 cgctactcga taccggcgta ccgcgtgaag cctgcgccgc taaagccgcg cgtctgctca
     8881 cccgcctgaa cgtgccggaa cgcctgtggc atctggcgcc atcgaccttc tccggcggcg
     8941 aacagcagcg cgtcaacatc gcccgcggct ttatcgtcga ctaccccatt ctgctgctcg
     9001 acgaacctac cgcctcgctg gacgccaaaa acagcgccgc cgtagtcgag ctgattcacg
     9061 aagccaaagc ccgtggcgcg gccatcgtcg gcatcttcca cgacgaagcc gtacgtaatg
     9121 acgttgccga ccgcctgcac ccaatggaag cctcttcatg attatcaata acgtcaaatt
     9181 ggtgctggaa aacgaagtgg tacacggttc actggaggtg caggacggcg aaatccgcgc
     9241 ctttgccgaa agccagagcc gcctgccgga ggcgatggac ggcgaaggcg gctggctgct
     9301 gccggggctg attgagctgc ataccgataa tctggataaa ttcttcaccc cgcgcccgaa
     9361 agttgactgg cctgcccact cggcgatgag cagccacgac gcgctgatgg tggcgagcgg
     9421 catcaccacc gtactggatg ccgtggcaat tggcgacgtg cgcgacggcg gcgatcggct
     9481 ggtgaatctg gagaagatga tcaacgccat cgaagagacg cagaaacgcg gcgtcaaccg
     9541 cgccgagcac cgtctgcatc tgcgctgcga actgccgcat cacaccacgc tgccgctgtt
     9601 tgaaaaactg gtgcagcgcg agccggtgac gctggtgtcg ctgatggacc actcgccggg
     9661 ccagcgccag ttcgccaatc gcgagaagta tcgcgaatat tatcagggca aatactccct
     9721 cactgatgcg cagatgcagc agtacgaaga agagcaactg gcgctcgccg cacgctggtc
     9781 gcagccgaat cgcgaatcca tcgccgccct gtgccgcgcg cgaaaaattg cgcttgccag
     9841 ccacgatgac gccacccacg cccacgttgc tgaatctcac cagcttggca gcgtgatcgc
     9901 cgaatttccc accacgttcg aagcggcgga agcctcgcgc aagcatggca tgaacgtgct
     9961 gatgggcgcg ccgaatattg tgcgcggcgg ctcgcactcc ggcaacgtgg cggccagtga
    10021 actggcgcag cttggcctgc tggatatcct ctcttccgac tactaccccg ccagcctgct
    10081 cgatgcggca tttcgcgtcg ccgatgacga gagcaaccgc tttacgctgc cgcaggcggt
    10141 gaagctggtg actaaaaatc cagcgcaggc gcttaatctc caggatcgcg gggtgattgg
    10201 cgagggcaaa cgcgcggact tggtgctggc gcatcgcaag ggcaatcaca ttcatatcga
    10261 ccacgtctgg cgtcagggta aaagggtgtt ctgatgatgg gaaaactgat ttggttaatg
    10321 gggccgtccg gctccgggaa agatagcctg ctggcggaac tccgcctgcg ggaacaaact
    10381 cagctactgg tggcgcatcg ctacatcacg cgcgacgcca gcgccggaag cgaaaaccat
    10441 atcgctctga gcgagcagga gttttttacc cgcgcggggc agaacctact ggccttaagc
    10501 tggcacgcta acggtctgta ttatggcgtc ggcgtcgaga tcgacctctg gctgcacgcc
    10561 ggattcgacg tgctggtcaa cggctcacgc gcccatctgc cgcaggcgcg ggcgcgctat
    10621 caatcggcgc tgctgcccgt ctgtttacag gtttcgccgg agatcctccg ccagcgcctg
    10681 gaaaaccgtg gccgtgaaaa cgccagtgaa attaacgccc gcctggcgcg cgccgcccgc
    10741 tatactccac aggattgcca tacgctcaac aatgacggca gcctgcgcca gtcggtcgac
    10801 acgctgctga cgctgattca tcagaaggag aaacaccatg cctgcttgtg agcttcgccc
    10861 ggccacgcag tatgataccg acgcggttta cgcgctgatt tgtgagctaa aacaggcgga
    10921 gtttgaccac cacgcatttc gcgtgggctt tagcgccaat ctgcgcgacc cgaacatgcg
    10981 ctatcatctg gcgctgctgg atggcgaggt tgtcggcatg atcggcctgc atttgcagtt
    11041 tcatctgcat catgtcaact ggatcggcga aattcaggaa ctggtagtga tgccgcaggc
    11101 gcgcggcctg aacgtcggta gcaagttgct ggcgtgggca gaagaagaag cccgccaggc
    11161 cggggccgaa atgaccgagc tttcgaccaa cgtgaagcgc cacgacgcgc accgtttcta
    11221 tctgcgagaa ggctacgagc agagtcactt ccgcttcacc aaggcgctgt aacatgagtc
    11281 tgaccctcac gctcaccggc accggcggcg cacagggcgt tccggcatgg ggctgcgagt
    11341 gcgcggcctg cgaccgagcg cggcgctcgc cgcagtatcg ccgccaaccg tgcagcggcg
    11401 tagtgaagtt taacgaggcg atcaccctga tcgacgccgg gctgcacgat ctcaccgatc
    11461 gctggtctcc cggatcgttc cagcagtttt tgctgacgca ttatcatatg gatcacgtcc
    11521 aggggctgtt tccgctgcgc tggggcgtgg gcgatccgat cccgatctac ggcccgccgg
    11581 atgaacaggg ctgcgacgat ctgtttaaac atcccgggct gcttgatttc agccacacgg
    11641 tggaaccgtt tgtggtgttt gatttgcagg ggttgcaggt cacgccgctg ccgcttaacc
    11701 actcaaaact cactttcggc tatttactgg aaacggcgca cagccgggtg gcgtggctgt
    11761 ctgacaccgc tggcttgccg gaaaaaacgc tgaaattttt acgcaataat cagccgcagg
    11821 taatggtgat ggattgcagt cacccgccgc gcgcggatgc accgcgtaat cactgtgatt
    11881 taaataccgt gcttgcgctg aatcaggtta tccgctcgcc acgggtgatt ctgacccata
    11941 tcagccacca gtttgatgcg tggctgatgg aaaacgcact accgtcaggg tttgaggtgg
    12001 ggtttgatgg gatggagatt ggggtggcgt gatgagaggg aatgtgcgcg ctggccccct
    12061 caccctaacc ctctccccag aggggcgagg ggaccgattg tgctcaatat tgaatattgc
    12121 gctcgttttc tccctctccc cattggggtg aggggcgatg cctgctccat acccaacctc
    12181 atcgcccata ctcatcttcc attctccgct cttcatcctc cagttgccga cgctcctgat
    12241 caagctggcg ctggcgatcg tccagctgcc tgcggcgatc ttcaaactgg cggcggcggt
    12301 cgtcatattg tctgcgccga tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat
    12361 cttcatagtc tcgcccacgg tcagggttat aagcgtcatt aatcgcctgc tgaatattac
    12421 caatagtatc gtcgataata tcagcctggg ccggaacggt ggacagcgtg agcagggtga
    12481 ataaaagaaa tagcggaaag cgtttcatta gccaacctca aaaagaaact ctatccacat
    12541 tagtcattac tcatccatgc aagtagtgga tgaatctcaa tttctccgct gctctattgc
    12601 cgtaatcgcc tccacgcgtt gttgatgacg accgccttcg tactgtgcgc ccagccacgc
    12661 atccacaatc atttttgcca gttcgaggcc aaccactcgt gaaccaaaag ccagtgcaac
    12721 ttcactggca taatgcggat aatcagtgcg ctctgacgac ctggtgtctt tatcaatcac
    12781 ctcactcatt gaccatgaat aaaaggctgg cacagcaacg cagaatcctg cggtgccagc
    12841 gacatgaccg cccattgctc cggtacgttt tttaaaaaga aacaccggtt gcattgataa
    12901 attgtgctca gtatttgccg aagtacaata tatataataa atatataaaa cacctcatgt
    12961 tatttacaga gataataaat tcgcgtaatt ttccctcttc agcacaaatg cctaataaaa
    13021 tggatcgttc agctcacact tccacttatt aaagtcgcaa attcccaatt atgataaccc
    13081 cagctttaaa tgacgtattt tgcatggatt acatatttac gacataaatg ataaatggag
    13141 atttacatga ataactctcg gttattccgt ttgagcagaa ttgttattgc gttaactgcc
    13201 gccagcggca tgatggtaaa taccgctaac gcgaaagagg aagcgaaagc cgccactcaa
    13261 tatacccaac aggttaatca gaattacgcc aaatcattac cgtttagcga tcgtcaggat
    13321 tttgacgacg ctcagcgtgg atttatcgct ccgctgctgg atgaaggtat tctgcgcgat
    13381 gctaatggca aaccatatta tcgcggggaa gattataaat ttgatatcaa tgcgcccgcg
    13441 cccgaaaccg ttaaccctag cctgtggcgt cagtcgcagt taaacggtat ttccggcctg
    13501 tttaaagtca ccgacagaat gtaccaggtt cgcggtcagg atatctccaa catcaccttt
    13561 atcgaaggtg ataccgggat tattgtcatc gacccgctgg tgacgccacc gagcgcaaaa
    13621 gccgcccttg acctttactt ccaaaatcgc ccgcaaaaac cgattgttgc ggttatttat
    13681 acccacagcc ataccgacca ctatggcggc gtgaaaggca ttatctctga agctgatgtg
    13741 aaatccggta aagtgcaggt tatcgcccct gctggcttta tggacgaagc catcagcgaa
    13801 aacgtactgg cgggtaatat tatgagccgc cgtgcacttt actcctacgg cctgctgctg
    13861 gcgcataacc ctcagggtaa catcggcaat ggtcttggcg taacgctggc atcgggctac
    13921 ccgagcatca tcgcaccgaa caaaaccatc accaaaaccg gtgagaagat gattatcgac
    13981 ggcctggagt ttgacttcct gatgacccca ggtagcgaag caccagccga aatgcacttc
    14041 tatattccgg ccctgaaagc gctgtgtacc gccgagaacg ccacgcatac cctgcacaac
    14101 ttctacactc tgcgcggcgc gaaaacccgc gacaccagca agtggaccga gtatctgaac
    14161 gaaacgctgg atatgtgggg taacgacgcg gaagtcctgt ttatgccgca cacctggccg
    14221 gtctggggca ataagcatat caatgattat attggtaaat atcgcgatac tatcaagtac
    14281 attcacgacc agaccctgca cctggcgaac cagggctaca ccatgaatga aatcggcgac
    14341 atgattaaac tgccgcctgc acttgccaat aactgggcca gccgtggcta ttacggttct
    14401 gtcagccaca acgcccgcgc ggtgtataac ttctatcttg gctattacga cggtaacccg
    14461 gctaacctgc atccgtatgg tcaggtggag atgggtaaac gttacgtgca ggcgctgggc
    14521 ggttctgccc gtgtcatcaa cctggcgcaa gaagcgaaca agcaaggtga ttaccgctgg
    14581 tcggcagaac tgctgaaaca ggtgattgcc gccaacccgg gtgaccaggt cgcgaagaat
    14641 ctgcaagcga ataactttga acagctgggc tatcaggccg agtccgccac ctggcgcggt
    14701 ttctacctga ccggcgcgaa agagctgcgc gaaggggtgc ataagttcag ccacggcacc
    14761 atcggttccc cggacaccat tcgcgggatg tcggtcgaaa tgctgttcga ctttatgtcc
    14821 gttcgcctcg atagcgcgaa agccgcgggt aaaaatatca gcctgaactt caatatgagc
    14881 aatggcgata acctcaacct gacgctgaac gatagcgtgc ttaactaccg taaaacactg
    14941 caatcccaag ctgacgcctc tttctacatc agccgtgaag atctgcacgc cgtgctgacc
    15001 gggcaagcca aaatggcgga tctggtaaaa gcgaagaaag ccaaaattat tggcaatggc
    15061 gcgaaactgg aagaaattat cgcctgtctg gataatttcg atttgtgggt gaatatcgta
    15121 accccaaatt aatgtcactc gcttttgggg gcgctgaagc ccccaaaagc atttatttta
    15181 aaaatatata cagccaatta ataaataaaa tatataagtt atgaatgcgg tgactgaata
    15241 aagctatttt tattcatcga gaaaatagcc atactgacgc cttcaatgga tattgatatt
    15301 cactacagat attgttcgca gcagacagga tgtatttaat atgccaacag ttctctctcg
    15361 catggcgatg caactcaaaa aaacagcctg gataattccc gtcttcatgg tttcgggatg
    15421 ctcattatct ccggcaatcc cggtgatcgg tgcttattat cccagctggt ttttctgcgc
    15481 cattgccagc cttattttga tgctcatcac gaggcgagtt attcagcggg caaatatcaa
    15541 tctggcattt gtcggaatta tttataccgc cctttttgct ctctacgcca tgctgttctg
    15601 gctggcattt ttctaatcta tttgagacgc gctcatggaa agtacgccga aaaaagctc
//
LOCUS       DAZOXR010000090        15501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZOXR010000090 DAZOXR010000000
VERSION     DAZOXR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.092"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            372..530
                     /locus_tag="ACHUNN_002488"
                     /pseudo
     CDS             372..530
                     /locus_tag="ACHUNN_002488"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            568..855
                     /locus_tag="ACHUNN_002489"
     CDS             568..855
                     /locus_tag="ACHUNN_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0576838.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1297..1830
                     /locus_tag="ACHUNN_002490"
     CDS             1297..1830
                     /locus_tag="ACHUNN_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR0576839.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2084..2767
                     /locus_tag="ACHUNN_002491"
     CDS             2084..2767
                     /locus_tag="ACHUNN_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFR0576840.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2940..3491
                     /locus_tag="ACHUNN_002492"
     CDS             2940..3491
                     /locus_tag="ACHUNN_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576841.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            3653..3907
                     /locus_tag="ACHUNN_002493"
     CDS             3653..3907
                     /locus_tag="ACHUNN_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR0576842.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            4074..4370
                     /locus_tag="ACHUNN_002494"
     CDS             4074..4370
                     /locus_tag="ACHUNN_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HFR0576843.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILVSPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQCEVPNETHKNVVIENSIKSQLI"
     gene            4367..4960
                     /locus_tag="ACHUNN_002495"
     CDS             4367..4960
                     /locus_tag="ACHUNN_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFR0576844.1"
                     /translation="MKNNNVPLYLWLLWCTLCLYITTPWQYLRYPYTDNLSLPYMLYF
                     CVVVVICIFPLLFRCVLLIVRSASKYDFTFFVKKKKGSKKSTIHLSPYADTRCPKSMR
                     KHWDVLLSLISKAINNDEIIVMHSHLFTPARINKLIKRLESYDLSFSVITYPRKTSLY
                     EKLSIPFIYLLFQWSFPHLHSDGMTVVIRPLQPPHTN"
     gene            4994..6061
                     /gene="pilL"
                     /locus_tag="ACHUNN_002496"
     CDS             4994..6061
                     /gene="pilL"
                     /locus_tag="ACHUNN_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HFR0576845.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            6061..6498
                     /gene="pilM"
                     /locus_tag="ACHUNN_002497"
     CDS             6061..6498
                     /gene="pilM"
                     /locus_tag="ACHUNN_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR0576846.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            6512..8194
                     /locus_tag="ACHUNN_002498"
     CDS             6512..8194
                     /locus_tag="ACHUNN_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR0576847.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            8187..9482
                     /gene="pilO2"
                     /locus_tag="ACHUNN_002499"
     CDS             8187..9482
                     /gene="pilO2"
                     /locus_tag="ACHUNN_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR0576848.1"
                     /translation="MADEDINPVVLLEDPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGKISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFGVIPDFNLKDGARQVSVTRPLPSLPGRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDEFFPLLQDTGVRLSNIHFELNGGTFSYSSEGHIYASK"
     gene            9469..9921
                     /gene="pilP"
                     /locus_tag="ACHUNN_002500"
     CDS             9469..9921
                     /gene="pilP"
                     /locus_tag="ACHUNN_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR0576849.1"
                     /translation="MRPSKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTAPLPSVSEQTAPKNRKELPIIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            9932..11485
                     /locus_tag="ACHUNN_002501"
     CDS             9932..11485
                     /locus_tag="ACHUNN_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR0576850.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PGINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWETRAPELRDD
                     ERDYLERHCNKDSLCSTDNIWFRNPHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            11498..12595
                     /locus_tag="ACHUNN_002502"
     CDS             11498..12595
                     /locus_tag="ACHUNN_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR0576851.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTQLIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGNF"
     gene            12613..13227
                     /locus_tag="ACHUNN_002503"
     CDS             12613..13227
                     /locus_tag="ACHUNN_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR0576852.1"
                     /translation="MLVENINATLTGNNKKNEPHDKGWGILEQGTIALVVLFVIVVVL
                     GSLYALRTRTNVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGAVPSGM
                     TVQGDKTSGTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTG
                     LTNGITLNSTAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            13237..13797
                     /locus_tag="ACHUNN_002504"
     CDS             13237..13797
                     /locus_tag="ACHUNN_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0576853.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            13782..14438
                     /locus_tag="ACHUNN_002505"
     CDS             13782..14438
                     /locus_tag="ACHUNN_002505"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0576854.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            14438..>15501
                     /locus_tag="ACHUNN_002506"
     CDS             14438..>15501
                     /locus_tag="ACHUNN_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR0576855.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT         4341 a         3745 c         3503 g         3912 t
ORIGIN      
        1 agatcattcg atcgtaaata gaataattct tagatgataa aaccatttgt tacacaaccc
       61 gccaattgtg taacaacagc ctctacaaga gcatatttgt tcatatgttc gtcagaactg
      121 cggattgtct ggacgagggt cagacaccgt ttcgtgcata tggaccagca tgttcgaacg
      181 ccacaaagca gaagcatcat gcctggatcg cttcggcgcg gagcgttaag ttttgacgct
      241 tttttgtatg ttttttaagc tgttagttga ctcggtgcgt tatactaagt accctttact
      301 ggtcacaggc aaaactgtga tcgggcgtag gaacccgcgt agtcatcgtc ggcgcagaag
      361 ttaatttctt catttggcgt cgttttttgc cataccataa agacaggaac ttgtatttct
      421 tgtcgtcatc tatggtagct caggcggggc aggcattagc ctggccggtt tccgatgacg
      481 ccggtattcc tacccccgtc tgggctatca ccaatgagcg taggaactcg gtgatagcgt
      541 ttttagttgt catcggagac cgttgctatg tcacctcaaa tgatacagaa actcccggcc
      601 atcactctgt tggaaggcat gtttcccgag ctatcaacaa accagcttaa agtctgcgtt
      661 ttttacgcaa tgggtgttcc ttacgacgcc attgctcaga actgtcggct ttctcccgaa
      721 acggtacgaa cctacctgaa gcgaagcctg aagaacttaa atctggaagg atatgatgca
      781 cttcgctctg ctgtgctgat gcgaacgttc gttttcatga ttagcaatac agctaaagaa
      841 aacgaaaaaa tgtgaacagg tgtccccatt aaaggggaca ctttcatgga aattgcattt
      901 tgcactttac aggcgatccc aaattgataa acataaacac caagcacaag ttactttaat
      961 aaagcaaatc acttcagacg gtgtcccagt tatttgtatc tggctcatgc ccgatatatg
     1021 gcgaccttac tctgaactga cgtgctgatg ttttatttcg cgtatcacaa tactgtgcca
     1081 ctgtattgct gatattagtg cttagccgtt ctttatctgt gagttcgcca aatcaccgat
     1141 acagaacgta ctagagtctg ctggttatat gcctgtcaga actgcagttt atccagatat
     1201 gggctggaca ctgcttcaga catatgattt agcatattcg cctgacatcg caaaatcgtc
     1261 catcatggat cggttttgcg cggagcgttg aacttcgtga atatagaaca cctgaataac
     1321 cggaactggt atcttgcgca atataataca gccggcaaaa acagagaatc tttattctca
     1381 tggctgaatg agcaaaacgt cgtaccatgg actccattaa taacaagaaa gataagacgg
     1441 gcagactcca ggtgctgtta ccgtgaaaga atattcgcta tttttccagg gtactttttt
     1501 attctggcta attttgacat tcacccagta tcagcattac gtcgacatag cgccttcatt
     1561 gatttcgtga agtttggcgg tgaaataaaa cccgtcaata aagatattgt tgatggatta
     1621 atgaagattt acccggaccc tgtgctaaat ccgggagcca gggaagagct gaatgcggct
     1681 tccagcatat ggctgacaaa agcacaatat cagtatctcc tcagaatgga aaatactctc
     1741 cagcctgagt cgaggatatc cctgctcctg gaactggtat caaatgccga acatcacggc
     1801 tttatggagc gccttgtaaa catcccatag ccagtatttc ccggccttcg ggccgggctt
     1861 ttttcaattc cagagaatat tccggagtga agcctgcacg aagccatccc caccatggac
     1921 tccccctgtc ttttgatgga accgtgtatt tcttaatggc gaaagaaata aaaggcttca
     1981 ccccagagta ttgattcgtt ttatcaaagg ccggcccgtg ccatcacgtg tggcggagag
     2041 aacggatacc ggtcttcttt tcatctcaca gtaggacatt cgcatgacca cgacagaaca
     2101 tcccgtgcgc cagttgctct ggtgtgcact gaatgccctg aaaacggcac aggaaaacca
     2161 caatataacc agtgaaaccg gattacggca ttacctgctt gaatggcttt ctggtgccag
     2221 cagacatcct gagttccgta gtctccctgg cgaaatcatg gcgttaaaag cattgaccga
     2281 aaaagatcgc aatattccca tcatcggaac gctgaatacg ctctttctca gctctgccac
     2341 tgtcggggaa tgtccgttgt ttcgttttcg ggctgcactg ggtaaattac agaaagcagg
     2401 ctggcgcaca tatgtctgcc catggcctga gagggtgttc aatgaatcca ttgaacgggc
     2461 ctgttccggg aaacgtcatc tgctacaact aagtcgcacc gaagagtgtt ttctaccgac
     2521 aggcgatatg agggcaccag ttacgttgca gctgatcact ccactacacc gcaaaactat
     2581 gcagcttctg gaaacggcag agttaatatt ctccgacgaa ggatttcagg tggtgagagg
     2641 atacgaacat caattcggga tcgaacgcag agaaaccctc tttcacaccc tatttatcgg
     2701 gctgcccagc ctgccagaag atgtctgggg tgcaacacag gagcagacga ctatcacatt
     2761 acactgagga tgtgtaatcc ccggcatacc ccgtttccag tcttcgcagc ctgtaccgcc
     2821 cttcctgccg tttccgacat tctttctgtc actactcgct tgtgccattt tttcgcaagc
     2881 ggcccccatc gacatgacgg tgcgagtctt tctttttacc ctgagaggaa attaacagca
     2941 tgaaaaaaca accactactc tggatgacga tagccgccgc actgctgtcc ggctgcacag
     3001 gccacaggga acatcacccg caggtgacat tcacggtcga taccgtgcga acagacacaa
     3061 cggccagcaa cccaatctgc ctggcggaaa aatacggaag ccttcttaat gagcgccatc
     3121 cggacctgtt tgccggtgct caggggagta tttcacgcag tgcatttttt aatgacagcc
     3181 tgacgctgac acctgaaacg ctccacggga tgatacagaa tacgccctgt gttgaatcac
     3241 tggtaacgtc cgtaggtccg gataccttca ctgcagatgt ctctcccggt gtcagaatgc
     3301 aggtatcacc ggatattcac ggtgcgcccg gaaatatcac tctgcgactc tcgaccacag
     3361 accagaaacg aaaacagact gttgaacaaa gcatttctct cagaccggaa cagtcccttg
     3421 tcgtgaatgg attcaccgga gacggagctg gcgaaaagcg cgtcgttctc atcacacctc
     3481 acgtgcgtta attcccaaca caacccgttt cccgtcttcg cagcctgtac cgcccttcct
     3541 gccgtttccg acattctttc tgtcactact cgcttgtgcc gttttttcgc aaccggtacg
     3601 tgcagttatg acggtacagc tgcttctttt cccgttccgg aaggagtctt tgatgccaca
     3661 gcaacatcct ggacgactgc aaatactggt cgtcgacgca cactgcaaac gcaggctgtt
     3721 ttcaacgaaa acaccgacag accccgatga gcttgcccga cgtttctgca cgccggataa
     3781 ctgtctcgtg gtggtactac gcgacaaccg atttctgttc cgactggagc gtgctcccgg
     3841 cagccactgc cgctggcaca agggcagcag ctcccgccat cagcatctac aggactggct
     3901 gtcgtaaaca caacaaaaac aaaagacctc gtcaaaacag gtgggggccg tgttcttttt
     3961 agttaaatta accatcgcga ttgcgattgt ctgtgtacac cttttaacga ttttaattta
     4021 gccgtttgtt gccatcttta ttacctctct cccttttatc atggagatta acgatgttta
     4081 ccatgttctt tctagttaaa ttaaccatcg cgattgtgat tgtctgtgga atcctttctc
     4141 cgattttagt ttcaccacat aacaaactgt ccacactttt ttggaagata ccaaacgcgt
     4201 tctggatttt cctgatttgc atatactttg caatcgccat ggcggtgtta atagaaagca
     4261 gtgaacagac acgcatccgc cgccaacaat atttccaaca gtgcgaagtg ccaaatgaga
     4321 cacataaaaa tgtggtgata gaaaacagta ttaaaagtca actaatatga aaaataataa
     4381 cgtaccatta tatctatggt tgctttggtg tacgctttgt ctgtacatca ctaccccatg
     4441 gcaatactta cgttatccat acactgacaa cttatcattg ccttatatgt tatatttctg
     4501 tgtggtggtt gtgatttgca ttttcccact acttttcaga tgtgtacttc ttattgtaag
     4561 gtctgcatct aaatacgact tcacattctt tgtgaagaag aaaaagggaa gcaagaaatc
     4621 aactatacat cttagcccct atgctgacac acgttgcccg aagtcaatga ggaaacactg
     4681 ggatgtattg ctttctctga tatcaaaagc aattaataat gatgaaatca ttgtcatgca
     4741 ttcacatctc tttactccag caagaataaa taaacttata aagagactgg aatcttatga
     4801 tttgagtttt tcagttatta catacccacg gaaaacaagt ctgtacgaaa aattatcaat
     4861 tccttttata tatctcttat ttcagtggtc tttcccgcat ttgcattcgg acggaatgac
     4921 agtcgtcatt cgtcctctcc agccgcctca caccaattaa caaacacgta tatacgaaca
     4981 cggagtctcc gttatgaaaa aaaactgtct gacacttata ctgccgtcat tattgtccgg
     5041 ctgtgccgga atttccggca gcaataacgt ggcaccatca gatacgcctg ctctggtttt
     5101 tgttgacgga caaatcagtg aaagtgctgg cgttctggca cttacgcaac aacgtctttc
     5161 tccacctaaa ataactcccc ccccgagagt cagcgtgcca gagacagtaa aaacagcaca
     5221 gcctgttagc gaatcccgac cgttaccttc accaacaata agtcagaatt tctcccggtt
     5281 aatcattaca ggcaagcctg gtagcgcccc tgtaacacta tcgccgacgg cccgtaaccg
     5341 gaccgtcagt cagtggataa aaacattact accggaagga tggaaattca gacaagagaa
     5401 tgcagccacg cccaagctga ataccaaact ggtttcatgg tctgccaatg atcagtggac
     5461 ccgttctctg aaccggttgc tggaagaaca acacctatgg ggccatctgg actgggataa
     5521 taaaacgttg accgtcacaa catcagctgc tgcaccggca caggacacgg caaccactat
     5581 ctcgccagca gccatggtaa ccacatccac ttcccctgaa tctccaacaa cagcaaatag
     5641 ccagaacaaa ccacgaaatc cattcagagg aaacagcgta tcttcgtcaa cccccgccgc
     5701 caccccaact ccaaccggct ccacagtcaa gtctatcccg ctcatgaccg ggacgcctgt
     5761 caaaccggta tctcagggta aagagtggcg tgcgccggca ggcaccacat taagagagaa
     5821 cattattaag tgggcagaag aaacaaaatg cgaaagcatg gcatcaacgc actggatggt
     5881 catatggcca ctttctgtaa ccgattaccg tctggatgcg cctttaatgt tccggggatc
     5941 atttgaatca gccatggagc aggtatttga gttatatcgg actgcgcaaa aacctttata
     6001 cgcacaggct tctcgcatgc agtgcatcat tacagtttct gatacaaggg atggtcgcta
     6061 atgggatggc tggtcatggc cgtcggcctg accattctga ttatcactgg tagctaccag
     6121 aatcagaaga tgtctgaaac cacaaacgcc cagcaatatg caagtgcgtc tgtatgggca
     6181 agtcagatac taatgattgc taacagaatc aatgatatca gatacgtatc aggccagcag
     6241 gacggagtaa tttcctcaga taaactggct ctccctgtca ctccggactc ccgaattaaa
     6301 catcagttac aacaggggcg tctgtgggta tggatgcctg aacaaccagg acttgttgaa
     6361 actttgcgtt caaaaagtcg tggttctgca ctaataggga tcttccaaaa cgggcaactc
     6421 acctggctat caggaactgc aaccggtctg acacccccgg caggcattac tgctggttct
     6481 gtcgtctacg ttaactaatc tggatatatt catgaaaaaa tcacaccagc gttcaatgaa
     6541 gctggcggtg ctcccctgca tgatcgccgt cgctctttcc atctccggat gtactttcag
     6601 cgaaatcaac aaaatgcaga aaaaagcaca ggaagactca gcacatgcac gggaaaaggt
     6661 atcagccctt tcggcccgta aatcgcaggc tctcacctgg ctcgataatc aatggataaa
     6721 ccctgttccg gtcgctcagg tatcaagaga gaaaaaacaa acagctccgg cctgctacat
     6781 cacgcaggca agaaaaggag agatcactct gcaggaactg gggcaacgta ttactgccgt
     6841 atgcggcatc cctgtaatca tcacgcctga cgcagccaat tcaactcttg aaggaggcgc
     6901 tacccgccag atgacaggaa cactaccagc accagatgaa aatggacgtc taccgttaag
     6961 cagcctgggc agcacaacaa tgactacctc cactcagcca ttaacgctga ataacctcat
     7021 gtggcaggga gatatcaatg gtcttctgga tctgatggcc agccggagtg gtctgtactg
     7081 gcgcatggat aatggtcgga ttgtattcta tctgactgaa accagaacgt atccacttca
     7141 tatgctgaac accaaaacca gcagcagttc cagtgtcagc tctggctcaa caagcacaat
     7201 gggggcaaca ggaggccagg ataactcagc atccggtgat gcaacgtcct ctcagagcac
     7261 aaccgttggt caggaatacg atctgtatga agacatccgg aaaactattg aagcaatgct
     7321 gacaccagaa aaaggccgtt actggttatc tgcatcgagc tcaacgctaa cagtcactga
     7381 tactcctgct gtccaggaag ccgtcgcacg atatgtggac gaacaaaaca gtattatgaa
     7441 ccgccaggta gccctgaacg tacaggttct gagcgtcagc aataccagaa acgagcagtt
     7501 cggtctggac tggaaccttg tttacaaatc gctacattcc gccggagcaa cgttgaacaa
     7561 tgcaagcgga gattttacag gcgctacatc tgcaggcgta tcaattctgg atacggcaac
     7621 agggaatgcc gccaaattca gcggttccag ccttctgatt aaagcactga gtgaacaagg
     7681 cgatgtcagc gttgtaactt cacaagaaag cactgtcaca aacctgacgc cggtacctat
     7741 ccagatggca gatcagacag tttacgtcgc ccagtcagca acaactacga ctacggatgt
     7801 aggagcaaca acaacattaa caccgggcat gatcaccact ggattcaata tgaccctact
     7861 gcctttaatt cagaaaacag gcaatctcca gttgcagatg aactttaatc tgtctgatcc
     7921 cccaactatc cgtagcttta cgtcaaaaga cggaaacagt tacatcgaaa tgccatatac
     7981 caaactgcgt tcactgagcc agaaggtcaa tctgaaagaa gggcaatcac ttgtcgttac
     8041 tggtttcgat cagaacaata cgacgacaag taaagccggt acgtttacgc cagcaaatcc
     8101 attatttggt ggttcacaaa ccgggaaaaa tgaacgcagc acgcttgtga tcatcattac
     8161 cccgactttc ccgtcaggag gcaataatgg ctgatgaaga tatcaacccg gtagtgcttc
     8221 tcgaagatcc taaagtaaat catcgcgtgt gggctgcctg tcttaaatgg actcctgtcg
     8281 taaaaaaaca gcgcgtcccc tcacatcata aacacaagtc tcatgtaaaa cccagacggc
     8341 ttacctccct gaaagtgact gtaggcagca gaaattcccg tggaaagatc agccgcctta
     8401 ccggcacagg aatactgacc aggccagaac gcaatcatta tttttccctg gcactggctt
     8461 tctgctcatg ggtcagaaat ggatacggtg tcttccggta cagcgataag gagctcttat
     8521 tccttgcttc cattaatggc cagcctgccg ttatggctga cttatcagga aatgatgccg
     8581 acgttgccca aaaggtcagc ctttttctgg cgatgaacga agagcccccc gaaaaatggc
     8641 aggttgtatc gtctctggaa catcctgata actgggaaag tattattacc cggttatcat
     8701 cagcagacct ccgccgttgc aaacttactg tcggcaatcg cagcaaattc acactgcctg
     8761 ccgtactgtt tttagtagca gccggcgcag gcactgtatt ctggatgact cagccagagc
     8821 cggatgtcgt accgacgccc gaggagatag ctgctcgggc aagattgcag tttaaaaagc
     8881 ccgatccacc gccagaactc ccgcatccat gggcatcaca acctgtaatc agtgattttc
     8941 tgaaagcctg cgcggacctt cgaaaaccgt caccggtcgc actggaaggc tggaaactta
     9001 ccggcggaac atgtactcct gagacattca cactgattta cgaacgacag cctggaggaa
     9061 ccattgaggg ttttctggca agaagtaaag aaatctttgg tgttattcct gactttaacc
     9121 ttaaagatgg tgcacgccag gtttctgtaa cccgtccact gccgtccctg cccggcaggg
     9181 atgaggctgt cccggccccc tcagaacaac tgatgcgggt gttcacgtgg tttcagaaaa
     9241 aacagttaac accggccatt aatgaaatag ccataccgga gccattacca ggcaacgatg
     9301 gtgaaccagc ccctgttcag aaatggaaag aatatcagtt cagtttatca actcctgtaa
     9361 atcctgatga gtttttccct ttacttcagg ataccggcgt ccgtctgagc aatatccatt
     9421 ttgaattaaa cggcggtact ttcagttatt cctcagaagg tcatatatat gcgtccaagt
     9481 aaattactca ttattccttc catatttttc ttctctggtt tttcatttgc cacaacacaa
     9541 cctcttgtca caataggaga acttgaagca caacaaaacc ggaatattct tttacaggca
     9601 aaagttcagg gtgcacagtt acagaaacaa cttgaagaat cagatgttac ttcatcatct
     9661 gaaactgttt caggtttctc tggtgtgacc gcccccttgc catcagtttc agagcaaacg
     9721 gcaccgaaaa acagaaaaga attgcccatc attatggaaa taaacggcaa agataaacgt
     9781 cttcatgccg ttcttcgcat ggctgatgga cgtcagacaa gcgtcaccac tggcagccag
     9841 cttccaggta catccgtcac ggtgaagtcg atctcattgt ctggcgtcac gttaagtgat
     9901 ggcactaccc ttactttctg agtaacgtga tatggataaa tataatctga aggatgctct
     9961 cctttttata tcccggggag acacccatga aattcttata gaaacaaacc agcggacaag
    10021 gcctgacgta caaagtaatc tccaggaact gctaaaatta tatccgggta tcaacccaaa
    10081 agtagtctcg ttgtcggagt tacaggaagc aggtcaggat gaaaaaaaca gaggcccgaa
    10141 agaacgaatt attcatctga aagatcttgc tgatgtcagt gaaagtgaga agaaagttct
    10201 atcttatttt gaaacggcca gaaaacttgg tgcttcagat attcattttt taatttctga
    10261 gtctattttc aaagttcgca tgagaatatt cggtgaactg cagactgtag atgaagatca
    10321 accagcactg ggctattcac tttgcgcaac agccattctg tcaatggctg atgtcactga
    10381 aaccagtttc ttccctcaaa gggaacagga tgcgcgcctg tccccacaat taatgcgaaa
    10441 aataggaata tttggtgcac gctacagtca cagacctacg ggagacggtc tgattgcagt
    10501 tatgcgcctg atacccgatg acggagataa agttcccacg tttaaacaac tgggctttat
    10561 cccggaacaa atccggttgc tgaacataat gctgcgccgc ccggaaggaa aaatagtcct
    10621 gtcaggtcca accggttccg gtaaatcgac aacgttacgc agcgcctgcc gtgtatatct
    10681 tgacgataat caggggcgac acctgctaac aatcgaagat ccgctggaag gacagatact
    10741 gggagcaaca cagactccga ttatctgcga caaatccgac gaagatgccg tcaaactggc
    10801 atggagtcgg gctatttcat cggcaatgcg acttgaccct gatgccatca tggaagggga
    10861 aatgcgcgat cttatctcga tgatgtcaac cacctacgcg gcacagaccg ggcacatagt
    10921 tctgacgaca ttacatacaa actcagctct gggtataccg gagcgaatga ttacgatggg
    10981 tatgaatgcc gatcttattt gcgatgcgca attactgatt gggatgatca gccagcgcct
    11041 tgtaccaact ctgtgtccct cctgccgtat tccatgggaa acgcgagcac ctgaacttag
    11101 agacgacgag cgtgattacc ttgagcggca ctgcaataaa gattcactct gcagtacaga
    11161 taatatctgg ttccgaaatc ctcacggatg ctctgaatgt aatcacgacg taatcattaa
    11221 cggcagaaag cgcggtgaaa taggtaaagg cctcaccggc aggactgtaa ttgctgaggt
    11281 gatagaacct gataaccgct tatttcagat tctgaaaaca cgcggcaaag ttgcagcacg
    11341 taaatactgg ctggaaaaca tgaaaggcat cagtcgtgta gagcatcttc ttcgccgtat
    11401 caatgaaggt ctggtagacc ctcttgaagc cgacagaatc atccctctgg acgaagacga
    11461 gcgcctcagc atcgatgatg tctaacagga gaacaacatg cgtgaaatga atttcagtca
    11521 acggctgagg cgtttcattg tcagaaaaac gttcagcgcc ccctacaggg tgcagtttta
    11581 cgaagccctg cgttttctgc tggaaaataa acagccatta aagaccgcac tggaacagat
    11641 gcgggatgcc tggacagact ttggacgaaa atggcatccc tttgctgaac tggccacaga
    11701 ctgcattgag tctcttcgtg aaaacagtgg cgaaaactct ctggaatata ctcttagttt
    11761 atgggttcct caggaagagg ccgcagtaat cagcgccggg ataagaagtg gttcaattgt
    11821 ggatgcactg caattcgcca ccactcttac agatgcaaaa gaacaaatcc atcaggctat
    11881 atggcaaatg gctatttatc cggtgggatt gttaatcatg atgacgggga cattatatgt
    11941 tctgaataca cagctaatac ctgaactgag caaaataagc tcccccgatt catggagtgg
    12001 tgctcttggt tttctttatg gattatctgt ttttgtcgat aattacggcg ctatatgtgc
    12061 cgttcttttt gcagtgatca caggtttaat atcatggtct ctgccaaact ggaaaagccc
    12121 tgacagtgtt cgtacttttg ccgacaagat aatgccatgg agtatttatc aggatataca
    12181 gggagcaaca tttctcctga atatggctgc acttctgaaa gccaaaatga cgacgctcaa
    12241 ctcactaaac attcttcagg agtttgcatc accatggtta agcacacgtc ttgacagcat
    12301 tatttaccgc gtccgccagg gggatcatct tggactggct ttgcgtcagt gcggttatca
    12361 gttcccatcc agggaagcag ccaacttcct gtctttatta cagggagatg gcgcaacaga
    12421 gctaatcagc aattatggtc aacgatggct tagtcaaaca cttcaacgtg taaagaaacg
    12481 ggcaaacgtg attcgtttaa ttatgctgat atttcttgtc atgtctctca tgttactggt
    12541 ttttgccatt atggatattc agtctattag tgacaacagc atgggtaact tttaaacaaa
    12601 acgaggatat ttatgttagt agaaaatatt aatgccactt taactggaaa taataagaag
    12661 aatgagcctc atgataaagg atggggaatt ttggagcagg gaacaatagc gctcgttgtt
    12721 ctttttgtta tagtcgttgt tctgggtagt ttgtatgcgt tacgtacaag aaccaatgtg
    12781 gcaactgaaa ccgcaaatat acaaacaatt atcacaagtg cccagagcct gctaaaaggt
    12841 agcgatggat atacatttac cagcagtgcc aaaatgacag gtgccctcat tcagatgggg
    12901 gccgttccct ctgggatgac agtacagggc gacaaaacat ctggtacagc aacgctctat
    12961 aacgcatggg gcggtgctgt aactgttgcc ccggcttcaa catcagggtt caacaacggt
    13021 tttaccgtta cctatgataa agtcccacag gatgcctgta tccagattgc cacacggatc
    13081 agtaaaactg gtttaactaa cggcattacc ctgaacagta ctgctcacag tgatggaaaa
    13141 gtcactactg aagaagccag tacgcaatgc aaggcagata atggcagcac tggtacaaac
    13201 aaacttatct tcaccatcaa tggttaagga gttttaatgt cagggggtac agtacccctc
    13261 agacttattt cattaatcct gcttttttct cttattccac ccgcccgagc ttcatacgta
    13321 gcccccaaac agatctggac caacaagtgg gacaattgct ttgccgctgc cggcgcgcgc
    13381 tatcaaattg aaccattatt attgaaggct atttctgccg gggaatcttc gttgaggccg
    13441 ggcgcaatta atataaataa ggacaaaaaa acaggtaaag ccagtagcac ggattatggg
    13501 ttgatgcaaa taaactcaac ccatattcca aagctgataa atatgggcgt aataaaaaag
    13561 tctgaggatt taatcaccaa accatgcctt aatatccata taggaagctg gatactggca
    13621 agacattttc agatatgtgg cgtcagctgg aattgccttg ggtcatacaa tgccggattc
    13681 cgtaaggaca gacatgaaac aagagagcaa tatgccaata aaatatggag aatttatcgt
    13741 gacatgaaag ggatatgtct tcccggacag ggaggcaggc aatgcagaca atcgtaggca
    13801 taagtctatc ccctgtttat atactgccat tgatgttcac attttccatt attggacgaa
    13861 ctctgctatt cagagttcgt tattttttat ctgatacggg acatctctgg tataagacac
    13921 accctgctgt tctgtcagga atatggctat acagtatagc tgtggcgtta attattttat
    13981 catcatcacc actgctctac cgtattcatg ctgtccttat tctttcgttc atactgcaaa
    14041 tggcggtaac ggatgcactg acaggtttat taccaggaac attcacccgg cgttttttaa
    14101 ttgcaggaat gctatcacaa atcacgactg atatatggtg gttccgtaca acagaatttg
    14161 ccactgctgc aattgtctta ttctgccttc ataaattagt taaccgccat cgtcttaata
    14221 ttggcactgg agacctgtgg cttattgcag gtattaccgc atggtcaggg ttatataacg
    14281 ccatatggtg tgtgttgctg ggcacaggag gatttgtctt atggcattca acatggtgca
    14341 taaaagggca taaagaaggc cctctgggtc catggctatg ttttggccat gtccttttgc
    14401 tgctggataa tctttatcaa ccactatggg taatctgatg aaaaaatatg accgaggctg
    14461 ggcatcgctg gaaactggcg cggctttact gattgttatg ctcctgattg cctggggagc
    14521 gggcatatgg caggactata ttcaaacaaa aggatggcaa actgaagcac gtcttgtcag
    14581 caactggacc agtgcggccc gctcttatat agggaaaaac tacacgactc tccagggcag
    14641 tagtaccaca acaactcctg ccgttatcac gacaaccatg ctgaaaaata ccggcttttt
    14701 gtccagcggg tttactgaga caaacagcga ggggcagcgg ttacaggcat atgtggttcg
    14761 aaacgcccaa aacccggaat tactacaggc aatggttgta tccagtggtg gcacacctta
    14821 tccagtgaaa gcacttatcc agatggctaa ggatattacc actggtcttg gtggatatat
    14881 ccaggacggc aaaacagcca caggtgcatt acgttcctgg tcagtagctt taagtaatta
    14941 tggtgccaaa agcggtaacg ggcatattgc cgtattgtta tcgacagatg aacttagtgg
    15001 tgcagctgag gacactgatc gtctttacag attccaggtc aatggtcgcc ctgacttaaa
    15061 caaaatgcac acggccattg atatgggatc aaataacctg aataacgttg gggcagtaaa
    15121 tgcccagaca ggtaatttca gcggcaatgt gaatggtgta aatggcactt tcagcggtca
    15181 ggtaaaaggc aatagcggaa actttgacgt aaatgtcacc gctggcggtg atatcagaag
    15241 taataatggt tggttaatta ctcgtaacag taaaggctgg ctcaatgaaa ctcacggtgg
    15301 cggattttat atgtccgatg gatcatgggt tcgaagtgta aacaacaagg gcatctatac
    15361 cggcggtcag gtgaaaggcg gtactgttcg ggctgatggt cgcctttata ctggtgaata
    15421 cttacaactg gaaagaactg ccgttgctgg cgcatcatgt tcgcctaacg gccttgtagg
    15481 ccgcgataat acaggggcaa t
//
LOCUS       DAZOXR010000091        15407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZOXR010000091 DAZOXR010000000
VERSION     DAZOXR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.093"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(359..1555)
                     /gene="hemY"
                     /locus_tag="ACHUNN_002507"
     CDS             complement(359..1555)
                     /gene="hemY"
                     /locus_tag="ACHUNN_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0576856.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1558..2769)
                     /gene="hemX"
                     /locus_tag="ACHUNN_002508"
     CDS             complement(1558..2769)
                     /gene="hemX"
                     /locus_tag="ACHUNN_002508"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0576857.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHUNN_002509"
     CDS             complement(2791..3531)
                     /gene="hemD"
                     /locus_tag="ACHUNN_002509"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0576858.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHUNN_002510"
     CDS             complement(3528..4469)
                     /gene="hemC"
                     /locus_tag="ACHUNN_002510"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0576859.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHUNN_002511"
     CDS             4856..7402
                     /gene="cyaA"
                     /locus_tag="ACHUNN_002511"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0576860.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHUNN_002512"
     CDS             complement(7442..7762)
                     /gene="cyaY"
                     /locus_tag="ACHUNN_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0576861.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7924..8066)
                     /locus_tag="ACHUNN_002513"
                     /pseudo
     CDS             complement(7924..8066)
                     /locus_tag="ACHUNN_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8309..8512
                     /locus_tag="ACHUNN_002514"
     CDS             8309..8512
                     /locus_tag="ACHUNN_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0576862.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8549..9373
                     /gene="dapF"
                     /locus_tag="ACHUNN_002515"
     CDS             8549..9373
                     /gene="dapF"
                     /locus_tag="ACHUNN_002515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0576863.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9370..10077
                     /locus_tag="ACHUNN_002516"
     CDS             9370..10077
                     /locus_tag="ACHUNN_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR0576864.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10074..10970
                     /gene="xerC"
                     /locus_tag="ACHUNN_002517"
     CDS             10074..10970
                     /gene="xerC"
                     /locus_tag="ACHUNN_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0576865.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10970..11686
                     /gene="yigB"
                     /locus_tag="ACHUNN_002518"
     CDS             10970..11686
                     /gene="yigB"
                     /locus_tag="ACHUNN_002518"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0576866.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11770..13932
                     /gene="uvrD"
                     /locus_tag="ACHUNN_002519"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11770..13932
                     /gene="uvrD"
                     /locus_tag="ACHUNN_002519"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0576867.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13987..14889)
                     /locus_tag="ACHUNN_002520"
     CDS             complement(13987..14889)
                     /locus_tag="ACHUNN_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR0576868.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(14972..>15407)
                     /locus_tag="ACHUNN_002521"
     CDS             complement(14972..>15407)
                     /locus_tag="ACHUNN_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0576869.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3514 a         4036 c         4150 g         3707 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagca aagaggatta cagaattatc tcataacaag
       61 tgttaaggga tgttatttcc cgattctctg tggcataata aacgggtaga tgctcattcc
      121 atctcttatg ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt
      181 gcttatcgtc cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg
      241 agtgaagcac ccactgttgt catacagacc tgttttaacg cctgctccgt aataagagca
      301 ggcgtttttt tatgcatcag aaaggtcccg gaggtgcttg cctccgggtg agaaggaact
      361 actgtggtgg gttgttctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt
      421 cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg
      481 gacgttgttt cagcgctgcg cggaaggcga gcgatgcttc ctgccattct ccgtgcttca
      541 tcagtgactg gcccagtgtg ctccacaaca gcgggcgatc gccgacgttt ttgatctgct
      601 ggcgcagcac tttttccagc tgttccggat tgttggtttt cagtcgagga atcggcagca
      661 gtaggcgatc gtcgtactgg cgtttcaggc catcgatgat aatttgctgg gcagtatcat
      721 gatcgtcaca ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg
      781 ttttccggct ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg
      841 cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac
      901 caacatgggc tttcgccatt gatgggatga tatccagcag agaactccat gcacctgtgc
      961 ggatatacgc ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca
     1021 gcttatccac gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac
     1081 gggtgatttc taccggaatg gtgtcattgc cggccagctc tgctgcgcgt tccagatgtt
     1141 ggttggcgcg tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca
     1201 ccaccggttg ttccgcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat
     1261 cgccttccgc cagtttcagc agcgcctgtt cggtctgctt acgggcacgg cgacgcttac
     1321 gtccgacaaa ccacccacgg gtgtgtgcgc ctgtgcggaa gatccgccgc agtagccact
     1381 caatggcaaa cagcactacc atcgccagaa tcaaaataat cgccaggccc gtgacgctgg
     1441 tttcgatatt gtagttgtca gtctggatca gcacgtaacc ctgatgaccg gcaatcatcg
     1501 ggccaaccac tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta
     1561 ttctccttgc ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc
     1621 aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt gtgccagcag
     1681 gttacgcacg cgggtctgca tcagtttttc cagcatcgcc tggctttgca gggtttccgg
     1741 aagatccatc gagatatttt gctggcttaa ctggtccacc tcgtcgagga acgctttggt
     1801 ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct ccagcgcctg
     1861 gcgataagtc tcttcctgat gacgcggtac agcttgtgct gcgaccagca ggcgagagcg
     1921 aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg cggtgtcatc
     1981 acgacggcga atcgtaatga agttgtccat aaagttctgc cagctttttt gcagattgat
     2041 acgccattcg ctgatggaac tggaaagctc ttcaccgtct gaatccatcg gcgaaccatc
     2101 gctgtcatta tcggccagac gcaggttatc tacctgattt gaaagctgat taagcttaag
     2161 gatgatgccg tcataatcca cctgcgatac tgcagaaagg ctggcgatat catcggtaat
     2221 tgcccgacga acggtaatca gactcgggtc attcatatcc gccaggctgg cgtctgcact
     2281 tttcagcaac gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag
     2341 tttcaccaga aaatcggcct gggccagcag ccaggtttta gcatcgctgc cggaaatggt
     2401 ggcgaccttt tgctggactt catccagctg ttttgccagc gtttcttgct gacgattcgc
     2461 ctgttcaagc tgcgtagcct gttgcttaat aatgccttcc agttcggctt tttggctctc
     2521 ctgggctttt tgcaatgccg tcagttggtt agccagggca tcgctggtgg cggtctgatt
     2581 gacggcctgt tgtttacccc agccatacaa accgacgccc gccgccagag caatagcgat
     2641 agccgccgcg ctgagaatca atgcggtatt gttcttactc tttttttctg ttgcgacagg
     2701 ttgtgacgtg gtgtccacgg cctccctagt ctcttcaacc acggcggagg ttttttcttg
     2761 ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt
     2821 ggcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga gtttcgccaa
     2881 acgctcactg acgaccaata gtcgacagtg tagtaaccag tgctcacgat accattgtgg
     2941 gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg tcgtcacctc
     3001 gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc atcgttgata
     3061 acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa ttagctcacg
     3121 accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta attcaggtaa
     3181 ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct gtccgcttac
     3241 ggtatgtagt gccagtgcgg tggtgcgtcc aatggcgaaa taattaggta gtcggggcca
     3301 tttacgatct tgctgatgta gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc
     3361 aaacaacaga tcgctctccc ccagcgctgc cagttgatca gcaagttgcg gtaattgttg
     3421 acccggagaa aactcaatca gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg
     3481 gctcactaac tcttctccag cgggagacgg gcgggtgaca aggatactca tgccggggcg
     3541 tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc
     3601 agcgaaatcc ccatttgttt ggcattttgc ggcgcaccgc ggcgttcacc gcgaataatc
     3661 tgcgaaccgt ccggcgcgcc gaccagcgca cgcagccaga tttcgccatc aataagctcg
     3721 gcgtagctac caattggcac ctgacatccg ccttcgagac gggtattcat ggcgcgttct
     3781 gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc gcgagtgcgc
     3841 gtatcatcaa ggcggcattc aataccgact gcaccttgtc ctaccgccgg aagagaaatc
     3901 tcgggtggta acgcggcgcg aatgcgggac tccagaccta aacgttttag tccggctacg
     3961 gcaagaatga tggcatcgta ttcgccgtta tctagtttgc tcaggcgagt gccgacgttg
     4021 ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca ctggcgacgt
     4081 aaactggacg tcccgacgat actgcctgct ggtaacgcat ccagactgtc atagttattg
     4141 gacacaaagg catcgcgagg atcttcacgc tcacaaatag tgaccagtcc cagaccttgc
     4201 gggaattcaa ccggcacatc tttcattgaa tgtacggcga tatcggcgcg attttcgagg
     4261 agcgcgactt ccagctcttt tacaaataag ccttttccgc ctactttcgc cagcggcgta
     4321 tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag gcccggatgg
     4381 ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt
     4441 gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat ccgtggtcca
     4501 tcctaacatc cttgccagag tgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa
     4561 ccgtaaaatg aggtctggca gtggatcctg acagacgttt cacgctgtcg taataaggaa
     4621 tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta cggtcaatca
     4681 gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact aaaaaaacca
     4741 ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg ccgcgtgtta
     4801 ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat acgtcttgta
     4861 cctctatatt gagactctga aacagagact ggatgccata aatcaattgc gtgtggatcg
     4921 cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc cgacattgtt
     4981 gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca aaggcatttg
     5041 cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg
     5101 aatgtcagta caggatccac cgaaaggtga gcttccaatt actggtgtat acaccatggg
     5161 cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg tctgtcatca
     5221 atcctggctc gatagcgaag agcgccagtt actacaacgt aaatgcagcc tgctggaaag
     5281 ctgggccgcc tcgctgggtg tggaagtcag cttcttcctg attgatgaaa accgctttcg
     5341 tcataatgaa agcggcagcc tggggggcga agattgtggc tccacccagc atatactgct
     5401 gcttgacgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat
     5461 ggtgccgtgc gacgaagaag agcattacga cgactatgtg atgacgcttt acgcgcaggg
     5521 cgtgctgacg ccaaatgaat ggctggatct cggtggctta agctcgcttt ctgctgaaga
     5581 gtactttggt gccagccttt ggcagctcta caagagtatc gattccccat acaaagcggt
     5641 gctgaaaaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac gtctgctggc
     5701 gaaagatatc aaacagcgtt tgcacgacgg cgagattgta tcgtttggcc tcgatccata
     5761 ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt tcacccgtct
     5821 ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa aaactcagcc gtgaacgcgc
     5881 ctgtgtaggc tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg gttgggacga
     5941 agctcgtctg gcaatgctcg ataaccgtgc taactggaag attgatcagg tgcgtgaggc
     6001 gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgatcc gctttgcgcg
     6061 tcgcaataac cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga cgcgtaagct
     6121 gtatgccgcg tttgaagcat taccaggtaa agtgacgctg gtaaacccgc agatttcacc
     6181 cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccgggccggg ctaaccgttc
     6241 aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc atcagccgct
     6301 ggaatataac cgttacctga ataaactggt ggcgtgggca tggtttaacg gtctgctgac
     6361 ctcgcgcacc cgtttgtata ttaaaggtaa cggcattgtc gatttgccta agttgcagga
     6421 gatggtcgcc gacgtgtcgc accatttccc gctgcgctta cctgcaccga caccgaaggc
     6481 gctctacagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg aatatgaccc
     6541 gacagcggcg ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg atgtcttcag
     6601 ctttggcgag aatcaaaatt gcctggtagg tagcgttgac ctgctgtacc gcaactcgtg
     6661 gaacgaagtg cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag ccctgaaaac
     6721 tattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag tcttctgtta
     6781 tagccagcat ctgcgcgggt taatccgtac tcgcgtgcag caactggttt ctgagtgtat
     6841 tgaactgcgc ctttccagca cccgccagga aaccgggcgt ttcaaggcgc tgcgcgtttc
     6901 tggtcaaacc tggggcttgt tcttcgaacg cctgaatgta tcggtacaga agctggagaa
     6961 cgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcctgt cagtgcaggt
     7021 tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgctagtg aagggatcat
     7081 ccagttcttc ttcgaagaaa cgcaagacga gaatggcttt aatatctaca ttcttgacga
     7141 aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc aaagaggaac tggtgcgtga
     7201 cgtcagtcgc ttctactcgt catcgcacga ccgcttcacc tacggctcaa gcttcatcaa
     7261 cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac aggtgattcc
     7321 gttccgcaca aaatctatcg gtaacatgcc gcctgccaat caggatcacg atacgccgct
     7381 attacagcaa tatttttcgt gatgaacgtg ccggaaagcg aggcttatcc ggcatgcaat
     7441 cttagcggaa actgactgtt tcacccgcct gctgcgtcgc cgcctgttcc agcaaatccc
     7501 agaaggtttc gccgctgcga tcgcaaatcc actcatcgcc tttcagatca aaatggtagc
     7561 cgccttgttt ggttgccagc catacctggt gcagcggttc ctggcggttg ataatgattt
     7621 tgctgccgtt ttcaaaggta atggtcagca cgccgccgtt gatttcgcag tcgatatcgc
     7681 tgtcgccatc ccagtcgtcc aggcgttctt caatggtcag ccagagttga tcagccaggc
     7741 gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg gcagtatagc
     7801 ggcatggggt cagggcttca aagtttgcac ctctgcggct gcgttccggc acgattcatc
     7861 cgtcaccgga ataatgatgt ctctgtgtag cgaaagattt gtctcttcat tagggcgcag
     7921 ttacaccacg cctttccctg tttctggtaa acattatgat caggtttacc gagcgagcat
     7981 cctcacgctg acggaactaa aaaagacaac aaacaaacca cattgcgata gtgcatagag
     8041 ccatcctggc gcgaggtgcc gatcacgaaa ctaccagcaa aacataaatc cccacgagta
     8101 agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg ctaaacgctt
     8161 gcttttacgt cttctcctgc gatgatagaa agcagaaagc agaaagcaga aagcagaaag
     8221 cagaaagcag aaagcagaaa gcagaaagca gaaagcagaa agcagaaagc agaaagcaga
     8281 aagcgatgaa ctttacaggc aatccataat gaaaaacgtg tttaaggcac tcactgtatt
     8341 acttactctc ttcagcctga cgggctgcgg tctgaaaggt ccgctctatt tcccgcctgc
     8401 agataaaaac gcaccgccgc cgaccaaacc ggtagagacg caaacgcaat ccacggtgcc
     8461 ggataaaaac gatcgcgcca ctggcgatgg tccatcccag gtgaattact aaaagtcagt
     8521 ttctgtaccc gcgtgattgg agtaaatgat gcagttctcg aaaatgcatg gccttggcaa
     8581 cgattttatg gtcgtcgacg cggtaacgca gaatgtcttt ttttcaccgg agctgattcg
     8641 tcgcctggct gatcggcacc tgggggtagg gtttgaccaa ctgctggtgg ttgagccgcc
     8701 gtatgatcct gaactggatt ttcactatcg cattttcaat gctgatggca gtgaagtggc
     8761 gcagtgcggc aacggtgcgc gctgctttgc ccgttttgtg cgtctgaaag gactgaccaa
     8821 taagcgtgat atccgcgtca gcaccgccaa cgggcggatg gttctgaccg tcaccgatga
     8881 tgatctggtc cgcgtaaata tgggcgaacc caacttcgaa ccttccgccg tgccgtttcg
     8941 cgctaacaaa gcggaaaaga cctatattat gcgcgccgcc gagcagacaa tcttatgcgg
     9001 cgtggtgtcg atgggaaatc cgcattgcgt gattcaggtc gatgatgtcg ataccgcggc
     9061 ggtagaaacg cttggtcctg ttctggaaag ccacgagcgt tttccggagc gcgccaatat
     9121 cggttttatg caagtggtta agcgcgagca tattcgttta cgcgtttatg agcgtggggc
     9181 aggagaaacc caggcctgcg gcagcggcgc gtgtgcggcg gttgcagtag ggattcagca
     9241 aggtttgctg gccgaagaag tacgcgtgga actccccggc ggtcgtcttg atatcgcctg
     9301 gaaaggtccg ggtcacccgt tatatatgac tggcccggcg gtacatgtct acgacggatt
     9361 tattcatcta tgaagcaacc aggggaagaa ctgcaggaaa cactcacgga gcttgatgac
     9421 cgggcggttg tcgattatct gattaaaaat cctgagtttt ttatccgtaa tgcgcgcgca
     9481 gtagaagcga tacgtgtgcc gcatccggta cgcggcaccg tttcgttggt cgagtggcac
     9541 atggcccgcg cacgtaatca tattcatgtt ctggaagaga acatggcgct gttgatggaa
     9601 caggctatcg ccaacgaagg cctgttttat cgcctactct acctgcagcg cagtctcacc
     9661 gccgccagca gtctcgacga tatgctgatg cgctttcacc gctgggcgcg cgatctcggc
     9721 ctggcaggtg cgagtctgcg cctgtttccg gatcgctggc gcttaggtgc gccgtcgaac
     9781 cacactcatc tggcattaag ccgtcagtct ttcgaaccgc tgcgtattca gcgtttgggg
     9841 caggaacagc actatcttgg gccgcttaac ggaccagagc tgctggtggt gctaccggaa
     9901 gcgaaagcgg tgggatcggt ggcgatgtcg atgctgggaa gcgatgctga tttgggtgtc
     9961 gtgctgttta ccagtcgcga tgccagtcac tatcaacaag ggcagggaac gcagttactt
    10021 catgaaattg cgctgatgtt gccggagctt ctggagcgtt ggattgaacg cgtatgaccg
    10081 atttacacac cgatgtagaa cgctacctac gttatctgag cgtggagcgc cagcttagcc
    10141 cgataaccct gcttaactac cagcgtcagc ttgaggcgat catcaatttt gccagcgaaa
    10201 acggcctgca aagctggcag caatgtgatg tgacgatggt gcgcaatttt gctgtacgca
    10261 gtcgccgtaa agggctggga gcagcaagcc tcgcgttacg gctttctgcg ctacgtagct
    10321 tttttgactg gctggtcagc cagaacgaac tcaaagctaa cccggcgaaa ggtgtttcgg
    10381 caccgaaagc gccgcgtcat ctgccgaaaa atatcgacgt cgacgatatg aatcggctgc
    10441 tggacattga tatcaacgat cccctcgctg tacgcgaccg tgcaatgctg gaagtgatgt
    10501 acggcgcggg tctgcgtctt tccgagctgg tggggctgga tatcaaacac ctcgacctgg
    10561 agtctggtga agtgtgggta atggggaaag gcagcaaaga gcgccgcctg ccgattggtc
    10621 gcaacgctgt ggcgtggatt gagcactggc ttgatttgcg cgacctgttt ggtagcgaag
    10681 acgacgcgct ttttctgtcg aaactgggca agcgtatctc cgcgcgtaat gtgcagaaac
    10741 gctttgccga atggggcata aaacaagggc tgaataatca cgttcatccg cataaattac
    10801 gtcactcgtt cgccacgcat atgctggagt cgagcggcga tcttcgtggt gtgcaggagc
    10861 tgctgggtca tgccaacctc tccaccacgc aaatctatac tcatcttgat tttcaacacc
    10921 ttgcctcggt gtacgatgcg gcgcatccac gcgccaaacg ggggaaataa tgcgttttta
    10981 ccggcctttg gggcgcatct cggcgctcac ctttgacctg gatgataccc tttacgataa
    11041 ccgtccggtg attttgcgca ccgagcgaga ggcgcttacc tttgtgcaaa attatcatcc
    11101 ggcgctgcgc agcttccaga atgaagatct gcaacgcctg cgccaggcgg tacgggaagc
    11161 ggaacccgag atttatcacg acgtgacgcg ttggcgtttt cgttcgattg aacaagcgat
    11221 gctcgacgcc gggctgagtg ccgaagaagc cagtgcaggc gcacacgcag caatgattaa
    11281 ctttgccaaa tggcgcagcc gaatcgacgt cccgcagcaa actcacgaca ccttaaaaca
    11341 gctggcgaag aaatggccgc tggtggcgat caccaacggt aacgcccagc cggaactgtt
    11401 tggtttgggg gattattttg agtttgtgct gcgcgctggc ccgcatgggc gctcaaaacc
    11461 gttcagcgat atgtactttt tggctgcgga aaaactcaac gtgccgatcg gcgagatctt
    11521 acatgttggg gacgatctca ccactgacgt gggtggggca attcgcagcg gaatgcaggc
    11581 ttgttggatc agaccggaaa atggcgatct gatgcaaacc tgggacagcc gtttactgcc
    11641 gcatctggaa atttcccggt tggcatctct gacctcgctg atataatcag taaatctgta
    11701 tatataccca gctttttggc ggagggcgtt gcgcttctcc gcccaaccta tttttacgcg
    11761 gcggtgccaa tggacgtttc ttacctgctc gacagcctta atgacaaaca gcgcgaaacg
    11821 gtggccgcgc cacgcagcaa ccttctggtg ctggcgggcg cgggcagtgg taagacgcgc
    11881 gtactggtgc atcgtatcgc ctggctgatg agcgtggaaa actgctcgcc atactcgatt
    11941 atggctgtga cgtttaccaa caaagcggcg gcggagatgc gtcatcgtat cgggcaactg
    12001 atgggcacca gccagggcgg catgtgggtc ggcaccttcc acgggctggc gcaccgtttg
    12061 ctgcgtgcgc accatatgga cgccaatcta ccgcaggatt tccagatcct cgacagtgaa
    12121 gaccagctac gcctgcttaa gcgtctgatc aaagccatga acctcgacga gaagcagtgg
    12181 ccgccgcgcc aggcaatgtg gtacatcaac agccagaaag atgaaggcct gcgtccgcat
    12241 catattcaaa gctacggtaa tccggtggag cagacctggc agaaggtgta tcaggcgtat
    12301 caggaagcgt gcgatcgtgc gggcctggtg gacttcgccg agctgctgct gcgcgctcac
    12361 gagttgtggc ttaacaagcc gcatatcctg caacactacc gcgaacgttt taccaacatc
    12421 ctggtggacg aattccagga taccaacaac attcagtacg cgtggatccg cctgctggcg
    12481 ggcgataccg gcaaagtgat gatcgttggc gatgacgacc agtcaatcta cggctggcgc
    12541 ggggcgcagg tagagaatat tcagcgtttc ctcaatgatt tccccggtgc tgaaactatc
    12601 cgtctggagc agaactaccg ctctaccagc aatattctga gcgccgcgaa cgccctgatt
    12661 gaaaacaaca acgggcgtct gggtaaaaaa ctgtggaccg atggcgcgga cggtgagcct
    12721 atttccctct attgcgcttt taacgaactc gacgaagcgc gttttgtggt taaccgcatc
    12781 aaaacttggc aggacaacgg cggggcgctt gccgagtgcg ccattctcta ccgcagtaac
    12841 gcccagtcgc gtgtactgga agaagcactg ttgcaggcca gtatgccgta ccgtatttac
    12901 ggcgggatgc gcttcttcga acgccaggaa atcaaagacg cgctctcgta tctgcgtctg
    12961 attgccaacc gtaacgacga cgcggcgttt gagcgcgtgg tgaatacgcc aacgcggggt
    13021 attggtgacc ggacgctgga cgtggtacgt cagacatcgc gcgatcgcca gttaacactc
    13081 tggcaggcat gtcgtgaact gttgcaggaa aaagccctcg ccgggcgagc tgccagcgcc
    13141 ttgcagcgat ttatggaatt aatcgatgcc ttagcgcaag aaactgccga tatgccgctg
    13201 catgtacaga ctgaccgggt aattaaagac tccggcctgc gtaccatgta cgagcaggag
    13261 aagggcgaaa aaggtcagac gcgtatcgaa aacttagagg aactggtgac ggcaacgcgc
    13321 cagttcagct acaacgaaga agacgaagat ttaatgccgc tgcaggcgtt cctctcccat
    13381 gcggcactgg aagcaggtga agggcaggcg gatacttggc aggatgcggt gcagttgatg
    13441 acgctacact cggcgaaagg gctggagttc ccgcaggtgt ttatcgtcgg tatggaagag
    13501 ggcatgttcc caagccagat gtcgctggat gaaggcggac gtctggaaga agaacgccgt
    13561 ctggcctacg ttggcgtaac ccgtgcgatg cagaaactga cgctgaccta cgcggaaact
    13621 cgccgtctgt atggcaaaga ggtttaccat cgcccgtcgc gctttatcgg cgagctgccg
    13681 gaagagtgtg tggaagaggt gcgcctgcgc gccacggtaa gccgcccggt tagccatcag
    13741 cggatgggta cgccgatggt cgagaacgac agcggctaca aactcggcca gcgcgtgcgc
    13801 cacgctaagt ttggtgaagg caccatcgtc aatatggaag gcagcggtga acatagccgt
    13861 ttgcaggtgg cattccaggg ccagggaatc aaatggctgg tggcggctta cgcccggctg
    13921 gagacggtgt aatttacaac tccgttgcag gatgcggtgc gccacaccgc atcctgcatc
    13981 agcaccttac ttagcgcact tcttcacatc aatattccag tcagcaccag acgcaacttg
    14041 atgtttctgc gcgaaattat ccatattgag cgccacggaa acattcaata agctgtttag
    14101 atacaccatc ggctggcctt ccggcacatc gccgaagctt gcgacatacg gcattttccc
    14161 tgcgtatttt tgctgggaac cttcggaaat ggttacacac aacgtgtcgt tgagtttgat
    14221 tcctgcctga ttcagcaact cgtcgctgat attgctccag acgttgccgt actgaatatc
    14281 gagaatcggg atattgccct tcacttcacc ttttgttgct ttggctttct gataagaaag
    14341 ttcgacaact tttgcaggaa gctctggccc cacctgttcg aacgtgatcg ctccggaagc
    14401 caggcgtgca ccggtgtagg catacacgtc acgaccgtgg aaggtatatg acttttcgga
    14461 acctttcagg cggttggttt tttcatcgat ttgccggacg ctctcaatac ccagcgattc
    14521 cgccaccaaa gtgagcgtac cgttatctgg tgaaacgaag tattgaccgt ttttggtttt
    14581 tagcactacc gatttacggt ctgtaccaac accaggatcg accacactga caaataccga
    14641 accttgcggc cagtaactgg cggtctgata caggcggtaa gcgccttccc agatgttata
    14701 cggtggaatt tcgtgtgtta aatcaaatat tttaagatta tggtcaacac cgaatgcgac
    14761 ccctttcatc gccgagacgg caccatcttt caggctaaaa tcagtttgta aaatcaaagc
    14821 gttatccgca taagcggatg atgatcccag acacgttaat aaaaataacg ttgcgagcgt
    14881 ttttttcatt tttgtttccc ttcatttatc tgatgatttt tcagctgtgt agccagcccc
    14941 agtttgtctg gcctcctcga gtgtacaatg cttaaccttt cctctcgacg gaaatcatgg
    15001 tgacaaaagg atagcgttgc cacgggattg ccccgccttt catatacata tgtgaaatcg
    15061 tgccatcaag ataaagcagc tgctcaacgt tcagtttcgc tttggcataa caggcaaaat
    15121 cataaaaatt tgtcgcctgc tggctcaaca aaaacacggc attcccatgt ttattaatcc
    15181 caacaccgtt acgaatttta cgtgaggcga cgttgggatg aatacgcgga ttaattacac
    15241 tgttttccat caacattggc cctgactgca ccgcaaactg aatctcttta ctggttttga
    15301 aggcatccag acgaacgatg tcgactttat ctcccgcgac ataaaacacg ccgccaggac
    15361 ggataaagaa attcccttca cctgaagcga gatttaacgc caccttc
//
LOCUS       DAZOXR010000092        15407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZOXR010000092 DAZOXR010000000
VERSION     DAZOXR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.094"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..454
                     /locus_tag="ACHUNN_002522"
     CDS             <1..454
                     /locus_tag="ACHUNN_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0576870.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="ACHUNN_002523"
                     /pseudo
     CDS             670..759
                     /locus_tag="ACHUNN_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUNN_002524"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUNN_002524"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0576871.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUNN_002525"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUNN_002525"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0576872.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUNN_002526"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUNN_002526"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0576873.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUNN_002527"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUNN_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0576874.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="ACHUNN_002528"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="ACHUNN_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0576875.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="ACHUNN_002529"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="ACHUNN_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0576876.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="ACHUNN_002530"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="ACHUNN_002530"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0576877.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="ACHUNN_002531"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="ACHUNN_002531"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0576878.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="ACHUNN_002532"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="ACHUNN_002532"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0576879.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="ACHUNN_002533"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="ACHUNN_002533"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0576880.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUNN_002534"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUNN_002534"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0576881.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUNN_002535"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUNN_002535"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0576882.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="ACHUNN_002536"
     CDS             13056..13436
                     /locus_tag="ACHUNN_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0576883.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUNN_002537"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUNN_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0576884.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUNN_002538"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUNN_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0576885.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15407)
                     /locus_tag="ACHUNN_002539"
     CDS             complement(15245..>15407)
                     /locus_tag="ACHUNN_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0576886.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4044 c         3851 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcag
//
LOCUS       DAZOXR010000093        15337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZOXR010000093 DAZOXR010000000
VERSION     DAZOXR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.095"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUNN_002540"
     CDS             <1..1015
                     /locus_tag="ACHUNN_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0576887.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUNN_002541"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUNN_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR0576888.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUNN_002542"
     CDS             1797..1904
                     /locus_tag="ACHUNN_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0576889.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUNN_002543"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUNN_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0576890.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUNN_002544"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUNN_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0576891.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHUNN_002545"
                     /pseudo
     CDS             complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHUNN_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHUNN_002546"
     CDS             complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHUNN_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0576892.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHUNN_002547"
     CDS             complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHUNN_002547"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0576893.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8365..10080
                     /gene="dld"
                     /locus_tag="ACHUNN_002548"
     CDS             8365..10080
                     /gene="dld"
                     /locus_tag="ACHUNN_002548"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0576894.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHUNN_002549"
     CDS             complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHUNN_002549"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0576895.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11225..11812)
                     /locus_tag="ACHUNN_002550"
     CDS             complement(11225..11812)
                     /locus_tag="ACHUNN_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0576896.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11982..12560
                     /locus_tag="ACHUNN_002551"
     CDS             11982..12560
                     /locus_tag="ACHUNN_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0576897.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12690..13451)
                     /locus_tag="ACHUNN_002552"
     CDS             complement(12690..13451)
                     /locus_tag="ACHUNN_002552"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0576898.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHUNN_002553"
     CDS             complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHUNN_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR0576899.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15164..15247
                     /gene="yohP"
                     /locus_tag="ACHUNN_002554"
     CDS             15164..15247
                     /gene="yohP"
                     /locus_tag="ACHUNN_002554"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR0576900.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3746 a         4173 c         3835 g         3583 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgcagtg
     3781 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca
     3841 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     3901 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcgtcc
     3961 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     4021 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
     4081 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     4141 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     4201 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
     4261 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
     4321 catcactaca ggccagcaat gccagcgccg cggccagcca gaaaccactg ccgaggctgg
     4381 tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc cgcctttcct gctccccaca
     4441 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg
     4501 gaataaaaca ggctgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
     4561 gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtaactga
     4621 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
     4681 tacgtagata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
     4741 ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt
     4801 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactctttca
     4861 gcaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacgcct ttcggatcgc
     4921 ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat
     4981 taacgccaga agtttgctgg gcagcggctt taatcgttac cgccgtgtcg ccgccagcca
     5041 gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc tttttcaaac gcgggtaacg
     5101 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctctttca
     5161 gataacgact caggtcggca agcgaagtga gtttgttttt ctctgccaca tcctgacgca
     5221 cggcgatggt ccaggtgtta ttcgctggtg cgggcgtcag ccagattaac ttgttttgct
     5281 ctgcatcgag ttttttgact ttctcgtaac cttgctgcgc gtttttccat gccgcatcgt
     5341 tttcatcttt aaagaagaaa gcgccattac cggtatattc cggatagata tccagttcac
     5401 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
     5461 gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat
     5521 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
     5581 acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
     5641 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag ttagcctgag
     5701 cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg cccggcgttt
     5761 cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt
     5821 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa
     5881 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
     5941 aggttcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
     6001 gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
     6061 cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta ctaccgtggc
     6121 cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt
     6181 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
     6241 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
     6301 cttgtcggca ttatacggac gaccggtatt cagatggctg tagtacaccg ggatctgccc
     6361 gacagaacgc gggaaggaca ttggcagctt gccggacggg ttgtaatcgc caaacaatac
     6421 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc
     6481 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
     6541 tttaccggtg gctttcagcg ccgtaatcaa gtcacgttgg ctttgcggaa tagtgatatc
     6601 ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag ccaccaccac
     6661 atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc
     6721 gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac
     6781 gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt taatcccggt
     6841 cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcacg
     6901 tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt
     6961 ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgcg cttctttacg
     7021 gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct
     7081 gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc
     7141 gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
     7201 atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc
     7261 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt
     7321 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt
     7381 ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
     7441 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
     7501 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
     7561 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat
     7621 tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
     7681 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
     7741 agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag
     7801 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
     7861 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
     7921 ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg
     7981 gttatccgga ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt
     8041 cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa
     8101 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
     8161 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
     8221 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
     8281 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
     8341 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
     8401 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
     8461 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
     8521 ggctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt
     8581 ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat
     8641 gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag
     8701 ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa
     8761 ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc
     8821 ggcggtattt gtaacaactc cggcggctcg ctggtgcaac gtggcccggc gtataccgaa
     8881 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg
     8941 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa
     9001 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
     9061 cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg gttatttgaa
     9121 tcttctggtt gcgccgggaa gctggcggtc tttgctgtac gtctagatac cttcgaagcg
     9181 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
     9241 cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatacaccgg
     9301 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
     9361 ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag
     9421 aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg
     9481 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg
     9541 ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat cgtggctggt ggattatttc
     9601 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta
     9661 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
     9721 gaagacattc tggcgttgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat
     9781 ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc
     9841 tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa
     9901 cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
     9961 catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga tccgaccaac
    10021 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
    10081 aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg tgccgtctgc
    10141 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgctggaaca
    10201 agcatcactt taccagtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
    10261 tatttgccaa acgcatccat cactaccagc gctaccggtt tattattgat aaccgtacgc
    10321 atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg aatattccag
    10381 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
    10441 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
    10501 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
    10561 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
    10621 ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc gcggttttct
    10681 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
    10741 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
    10801 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    10861 ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag atcaacaatc
    10921 atcgcgctac cggaggcaat ttcaggttgt gaagcggtgg tagcggctgc cgttttcgca
    10981 acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    11041 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg caccgccgga
    11101 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    11161 gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
    11221 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg agcagcactt
    11281 ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct tcctctttgt
    11341 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
    11401 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa
    11461 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    11521 gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca gccagcataa
    11581 cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    11641 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    11701 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    11761 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    11821 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    11881 ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag
    11941 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
    12001 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    12061 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    12121 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcggtttg
    12181 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    12241 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    12301 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    12361 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    12421 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    12481 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    12541 agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca
    12601 tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc aaaattttac
    12661 ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac
    12721 ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc ctctgagcac
    12781 agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa
    12841 ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc
    12901 ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgttttg
    12961 gttaacccgc cgagcgcatg tttagcggct gtgtaagcgc tggcatccgg cagcggcgta
    13021 tgttcatgta ccgacgtaat gttgatgata cgaccgccct gcccttgttt caccatctgg
    13081 cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    13141 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    13201 agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc accttctggc
    13261 agtttaccga gctccagctg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    13321 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccctgc
    13381 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aataatcgca
    13441 acctgtgcca tcaagttctc cacttaacgc tgaataaacg ttaagtatag aaggcgcata
    13501 tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatagaag catccagcca
    13561 ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc tggcttcgct
    13621 gacgcgggaa ccaacaatga tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg
    13681 caaggcatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg
    13741 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    13801 agacaagttg ctttcggctt ttgcgatatt gagattggcg ttaagacgac cactatcgaa
    13861 aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac ggaacagatc
    13921 gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc
    13981 cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa
    14041 atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc ttgccacttt
    14101 cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa
    14161 ccgtgcctca ataattttca ttttccccgc gacatcgttg agctgctgcc gggttttgct
    14221 ggcattaata tcggtttcca caccttcaac tgaagaagta atcccgttct gatatagctg
    14281 gcgatcggtc gcgataatgg tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa
    14341 cgccgcctgg gtttgccact cccagtacag gcgggctacg ctgccagcca gcaattggcg
    14401 ggtttgctcg cgttccgccg cccgtgcttt aaccgtaccc aggcgggcag taacctccgc
    14461 ccgattcttt ccccagatat cgagatgcca gcccgccgtt aagccaaaag taccgttggt
    14521 gtaccacggg ccggtcgtac ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc
    14581 ttctgccgac attttttgcc gctccatatc cgccgaaaag tcgatctgcg gaccatcctg
    14641 agtggcaact gccttcgcct gggcttcagc tagctgaatg cgctgttcag ccacctgcat
    14701 atccggtgcg ttctgtagtg cattgttaat taatgaagtg agctgattat cgtgatactc
    14761 cagccaccat tggctgtctg gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac
    14821 ttgtgcagcg ggcgtttgct ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc
    14881 ggccagcatc agtaacagcg gaaaacaggc gatggctgga taaaaggaat cacgattcat
    14941 gggggaataa tcaggtaaga aacggtgcgc ggagattacc gtgtgttgcg atatattttt
    15001 tagtttcgcg tggcaataca tcagtagcaa taaaacgaca tacccagaaa aatatagact
    15061 aagtaaatga tatcttccga tttatcttaa tcgtttatgg ataacggcaa agggcttcgt
    15121 tttttcctat acttattcag cactcacaaa taaaggaacg ccaatgaaaa ttatactctg
    15181 ggctgtattg attattttcc tgattgggct actggtggtg actggcgtat ttaagatgat
    15241 attttaaaat taattaatgt catcaggtcc gaaaataacg agaatatttc agtctctcat
    15301 cctgttgcgc tcctgtcatg tgcattgctt catataa
//
LOCUS       DAZOXR010000094        15337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZOXR010000094 DAZOXR010000000
VERSION     DAZOXR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.096"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            99..851
                     /locus_tag="ACHUNN_002555"
                     /pseudo
     CDS             99..851
                     /locus_tag="ACHUNN_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(892..1824)
                     /locus_tag="ACHUNN_002556"
     CDS             complement(892..1824)
                     /locus_tag="ACHUNN_002556"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0576901.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2188..4974
                     /gene="polA"
                     /locus_tag="ACHUNN_002557"
     CDS             2188..4974
                     /gene="polA"
                     /locus_tag="ACHUNN_002557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0576902.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHUNN_002558"
     CDS             complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHUNN_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0576903.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6285..6451
                     /locus_tag="ACHUNN_002559"
                     /pseudo
     CDS             6285..6451
                     /locus_tag="ACHUNN_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6570..7079
                     /gene="yihI"
                     /locus_tag="ACHUNN_002560"
     CDS             6570..7079
                     /gene="yihI"
                     /locus_tag="ACHUNN_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0576904.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7268..8641
                     /gene="hemN"
                     /locus_tag="ACHUNN_002561"
     CDS             7268..8641
                     /gene="hemN"
                     /locus_tag="ACHUNN_002561"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0576905.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8831..8941)
                     /locus_tag="ACHUNN_002562"
     CDS             complement(8831..8941)
                     /locus_tag="ACHUNN_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0576906.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHUNN_002563"
     CDS             complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHUNN_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0576907.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHUNN_002564"
     CDS             complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHUNN_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0576908.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHUNN_002565"
     CDS             complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHUNN_002565"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0576909.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13479..15302
                     /gene="typA"
                     /locus_tag="ACHUNN_002566"
     CDS             13479..15302
                     /gene="typA"
                     /locus_tag="ACHUNN_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0576910.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4004 a         3829 c         3937 g         3567 t
ORIGIN      
        1 cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac
       61 accatcatac actaaatcag taagttggca gcatcaccag cgagtcgaaa tttaatcaga
      121 ttaacattat caatcacggg gaagacgttg cccaaatcga tgctttcgtt gcaaaaacca
      181 tgctggatcg cgttaaagac aaagattata tcaatgtcaa tctgacctac gaacttgata
      241 agttaacaaa agggaatcag caactcggta gtggtgagtg gtcattgatt gctgaatcta
      301 ttgatccctc agcagtgcgc caatttatca tccagtataa cattgctatg cagaagcagc
      361 ttgctgtaca ccctgagtta gcaaacgatg aagttgctct gcaagaagtg aatgctgcat
      421 tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc gaccattaaa caaccggtaa
      481 gatggaagaa cgcactcggc gaactaaatg ccaatctgga tatcagtatt gccgacccag
      541 ccaaatcttc atcatccaca aacaaagata tcaaattgct caattttgat gtgaagttac
      601 cgcttaatgt cgtcacagaa accgcaaaac agcttaattt atctgaagga atggatgcgg
      661 aaaaagcgca aaagcaggct gataaacaaa tcagcgggat gatgacattg ggtcagatgt
      721 ttcagttaat cacgattgac aacaataccg cctcgctgca gctgcgttat acaccgggta
      781 aagttgtgtt taacggacag gagatgagcg aagaagaatt tatgtctcgt gccggacgtt
      841 ttgttcatta aaatactcac tcgcctctgc tcattcagag gcgagtaaaa cttacttatt
      901 ctgacgctgt gatgacatga gagacgtcag aagccggtct ttttcctgcc acaatgagtt
      961 cagccattgc tgaaaatggc gcttaaagct tttgtcattg atgtagtcgc catgtaactc
     1021 atcggcaata ggctgtaaat ccacatgtac gacaatccgc gtcagtttac cgcttaacat
     1081 atcgaagaac ggctgacggt tattgtccgg ataacacagc gtaacattaa gcagtttatc
     1141 gaattgttta cccagtacat ttagcgccat cgcaatgcct gccgcctttg gtggcaacaa
     1201 gttttgaaaa gtggagtggg tttgctgatg tttttcttgc gtgaaacggg agccttcaac
     1261 gaaattgaca atggtggtgg gatgcaggcg aaacttttca caagaacgcc gagtggtttc
     1321 aacatcttta ccacgtcgct ccggatgacg taataaataa gcgcgggaat aacgcttcat
     1381 aaacggcata tccagcgccc agcacgccag gccaagaaat ggcacccagg ccagctgttg
     1441 cttgaggaaa tatttattca tcggaatgtg cttacgaaac agcacgcaca gtacgacaat
     1501 atctgcccaa ctacggtggt tacaaataag cagataccag ttcttcttac ttaacccttc
     1561 cagcccgtga acttcccatt gcaggtgtgg gttcagatgc agtaataccg ccagaccttc
     1621 acaccagcaa tacatcataa aatcacaaaa acgcgaaacc tttcgccaaa tgactggcac
     1681 aggtagcaat agttttacaa tcccggcaat gatgatcggg acggaacaaa aaatagtgac
     1741 caaaatggtc aatacgatac tcagcaaaag ggttatcgca gcgagtattc tcgtcataat
     1801 gaatttattc aaaaggttag ccatagacag tgcgctaaag aagcgcaagg cgcagatttt
     1861 agcagaaaac gacccaaata acggatgatc cttaaggaga aaaataatcc atatctatcc
     1921 acattagaaa aaatcccatt atctcaatta ttagggatgg ctttattttt aactgcatga
     1981 aaaacaaaga caaacatcat gctgtaaaaa gcatgataat aaattaaaag cgatgtaaat
     2041 aatttatgca caaagttatc cacatgacga tttgcgagcg atccagaaga tctacaaaag
     2101 attttcacga aaagcggtga aaaactcatg ttttcatcct gtctgtggca tcctttaccc
     2161 ataatctgat aaacaggcac ggacattatg gttcagatcc cccaaaatcc acttatcctt
     2221 gtagatggtt catcttatct ttatcgcgca tatcacgcgt ttcccccgct gactaacagc
     2281 gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca tgctgcgcag tctgatcatg
     2341 caatataaac cgacgcatgc agcggtggtc tttgacgcca agggaaaaac ctttcgtgat
     2401 gaactgtttg aacattacaa atcacatcgc ccgccaatgc cggacgatct gcgagcacaa
     2461 atcgaacccc tgcacgcgat ggttaaagcg atgggactgc cgctgctggc ggtttctggc
     2521 gtagaagcgg acgacgttat cggtactctg gcgcgcgaag ccgaaaaagc cgggcgtccg
     2581 gtgctgatca gcactggcga taaagatatg gcgcagctgg tgacgccaaa tattacgctt
     2641 atcaacacca tgacgaatac catcctcgga ccggaagagg tggtgaataa gtacggcgtg
     2701 ccgccagaac tgatcatcga tttcctggcg ctgatgggtg actcctccga taacattcct
     2761 ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc tgcaaggtct tggcgggctg
     2821 gatacgctgt atgccgagcc agaaaaaatt gctgggttga gcttccgtgg cgcgaaaaca
     2881 atggcagcga agctcgagca aaacaaagaa gttgcttatc tctcatacca gctggcgacg
     2941 attaaaaccg acgttgaact ggagctgacc tgtgaacaac tggaagtgca gcaaccggca
     3001 gcggaagagt tgttggggct gttcaaaaag tatgagttca aacgctggac tgctgatgtc
     3061 gaagcgggca aatggttaca ggccaaaggg gcaaaaccag ccgcgaagcc gcaggaaacc
     3121 agtgttgcag acgaagcgcc agaagtgacg gcaacggtga tttcttatga caactacgtc
     3181 accatccttg atgaagaaac actgaaagcg tggattgcga agctggaaaa agcgccggta
     3241 tttgcatttg ataccgaaac cgacagcctt gataacatct ctgctaacct ggtcgggctt
     3301 tcttttgcta tcgagccagg cgtagcggca tatattccgg ttgctcatga ttatcttgat
     3361 gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc taaaaccgct gctggaagat
     3421 gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc gcggtattct ggcgaattac
     3481 ggcattgagc tgcgtgggat tgcgtttgat accatgctgg agtcctacat tctcaatagc
     3541 gttgccgggc gtcacgatat ggacagcctc gcggaacgtt ggttgaagca caaaactatc
     3601 acttttgaag agattgcggg taaaggcaaa aatcaactga cctttaacca gattgccctc
     3661 gaagaggccg gacgttacgc cgccgaagat gcggatgtca ccttgcagtt gcatctgaaa
     3721 atgtggccag atctgcaaaa acacaaaggg ccgttgaacg tcttcgagaa tatcgaaatg
     3781 ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg tgaagatcga tccgaaagtg
     3841 ctgcacaatc attctgaaga gctcaccctt cgtctggctg agctggaaaa gaaagcgcat
     3901 gaaattgcag gtgaggaatt taacctttct tccaccaagc agttacaaac cattctgttt
     3961 gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg gcgcgccgtc aacgtcggaa
     4021 gaggtactgg aagaactggc gctggactat ccgttgccaa aagtgattct ggagtatcgt
     4081 ggtctggcga agctgaaatc gacctacacc gacaagctgc cgctgatgat caacccgaaa
     4141 accgggcgtg tgcatacctc ttatcaccag gcagtaactg caacgggacg tttatcgtca
     4201 accgatccta acctgcaaaa cattccggtg cgtaacgaag aaggtcgtcg tatccgccag
     4261 gcgtttattg cgccagagga ttatgtgatt gtctcagcgg actactcgca gattgaactg
     4321 cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga ccgcattcgc ggaaggaaaa
     4381 gatatccacc gggcaacggc ggcagaagtg tttggtttgc cactggaaac cgtcaccagc
     4441 gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga tttatggcat gagtgctttc
     4501 ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc agaagtacat ggacctttac
     4561 ttcgaacgct accctggcgt gctggagtat atggaacgca cccgtgctca ggcgaaagag
     4621 cagggctacg ttgaaacgct ggacggacgc cgtctgtatc tgccggatat caaatccagc
     4681 aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta acgcgccaat gcagggaacc
     4741 gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg cgtggttaca ggctgagcaa
     4801 ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg tatttgaagt tcataaagat
     4861 gatgttgatg ccgtcgcgaa gcagattcat caactgatgg aaaactgtac ccgtctggat
     4921 gtgccgttgc tggtggaagt ggggagtggc gaaaactggg atcaggcgca ctaagattcg
     4981 cctgaacatg cccttttttc gtaagtaagc aacataagct gtcacgtttt gtgatggcta
     5041 ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa agtgctttct gaactgaaca
     5101 aaaaagagta aagttagtcg cgtagggtac agaggtaaga tgttctatct ttcagacctt
     5161 ttacttcacg taatcggatt tggctgaata ttttagccgc cccagtcagt aatgactggg
     5221 gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg atgcgctatg tttatcaggc
     5281 caacggtaga attgtaatct attgaattta cgggccggat acgccacatc cggcacaagc
     5341 attaaggcaa gaaaattatt cgccgtcctg cgtttcttct acaggctgca tctcgctaaa
     5401 ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt ttcttcaacg aagaaaacgt
     5461 ttcaacctgc acatcaccgt taaacgccag tacagcttca cgcaccatat tcaattgcgc
     5521 tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc agcaccagaa cggcgatatt
     5581 gctgtctacc gcccactcaa tcatctgctg atccaaatct ttcagcggat ggcgaatatc
     5641 cattagcacc accagacctt gcaggctctg acgtttttcg aggtattcgc cgagcgcacg
     5701 ctgccatttg cgcttcatct cttccgggac ttccgcataa ccgtacccag gcaagtcaac
     5761 cagacgcttg ccgtcagcca cttcaaacag gttgataagc tgggtgcgcc ctggggtttt
     5821 tgaggtacga gccaggcttt tctggttagt cagcgtgttc agcgcgctgg atttacctgc
     5881 gttggaacgg cctgcaaaag ccacttcaat tccggtatcg gaaggtaggt ggcgaatatc
     5941 aggcgcactc atcacaaaat gcgtctgttg ataattcaaa ttagtcaaag cggtcgtctc
     6001 cgtcagtcaa agctttgtgg cgattatacc tgaaccataa taaaaggcgg atttttcggc
     6061 gtgagcgttg taagtaaaag ccatacgctt tgtgagacat tgccgatagt ctttatgcga
     6121 aatagcagag aaaattctgc gatgcatgtc aaataagcta tataaatcag tgaattgctt
     6181 ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt tgattgtttg tttaaagcaa
     6241 aggcgtaaag tagcacccat agagcgagga cgctaacagg aacaatgact caggatgagg
     6301 gtcaggagcg ccaggaggcg aagacagagg attgtcagga agacaaacgt ccggagacgt
     6361 aattaaacgg aaatggaatc aacacggatt gttccggcta aaggaaaaac agggtgtgtt
     6421 ggcggcctgc aaggattgta agacccgtta agggttatga gtcaggaaaa aaggcgacag
     6481 agtaatctgt cgcctttttt ctttgcttgc tttctgttag attccgccgc aaatctatac
     6541 tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc atcttcaaac tcacgcagca
     6601 aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct ggatcaggaa gctcgtgatc
     6661 gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag ccgcgcagcg ggcggaaaca
     6721 ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc acgtattggc agtaaaaccc
     6781 ctattccatt gggcgtggct gaaaaagtca ccaaacagca caaaccgaag agtgagaaac
     6841 ctatgctttc accgcaggcg gagttggaat tactggaaac ggatgagcgt ctggatgcgc
     6901 tgctggaacg tctggaagca ggcgaaaccc tgagcgccga agagcaatcc tgggtggatg
     6961 ctaaactgga tcgtattgat gagttgatgc agaaactcgg cctctcttat gacgatgacg
     7021 aagaagagga agaagacgag aagcaagaag acatgatgcg tctgctgcgg ggtaactaac
     7081 ggattgccac cgtgggcctt cccgttctgc tgataaccct tccggttata tgttatctgg
     7141 tgtggttatt cgttaaacta cagcggttgt cgcggcgaca aaagtggctg cgcaaccggc
     7201 tgatgacccg aaacgggcat ccgccggtac gccgtagccg ccagagacgc catcggaagg
     7261 agtgagcatg tctgtacagc aaatcgactg ggatctggcc ctgatccaga aatataacta
     7321 ttccgggcca cgatacacct cgtacccgac cgcgctggag ttttcagaag acttcggcga
     7381 acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt ccattatctc tctacgtaca
     7441 tatcccgttc tgccataagc tttgttactt ctgcggttgc aataagattg ttactcgcca
     7501 gcagcacaag gccgatcagt atctggacgc gctggagcaa gaaatcgtcc atcgtgcacc
     7561 gctgttcgcc gggcgtcacg tcagccaatt gcactggggc ggcggaacgc cgacgtatct
     7621 gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc gaaaacttcc agttcaatgc
     7681 cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc gaactggatg tactcgatca
     7741 tttacgcgcc gaaggcttta atcgcctgag catgggcgtg caggacttca acaaagaagt
     7801 gcaacgtctg gttaaccgcg agcaggatga agagttcatc tttgcactgc ttaaccatgc
     7861 gcgtgagatt ggttttacct ccaccaacat cgacctgatt tacggcctgc cgaaacagac
     7921 gccggagagt ttcgccttta ccctgaaacg tgtggcggag ctgaaccccg atcgtctgag
     7981 tgtctttaac tacgcgcatc tgccgaccat ttttgctgct cagcgcaaaa tcaaagatgc
     8041 tgacctgccg agtccgcagc aaaaactcga tatcctgcag gaaaccatcg ccttcctgac
     8101 gcaatcgggc tatcagttta tcggtatgga tcactttgcc cgtccggatg acgagctggc
     8161 ggtggcccag cgtgaaggcg tgctgcatcg taacttccag ggctacacca ctcagggcga
     8221 taccgatctg ctggggatgg gcgtttccgc catcagcatg attggcgact gctacgcgca
     8281 gaaccagaaa gagttgaagc agtactatca gcaagtggat gaacaaggca atgctctgtg
     8341 gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc gatgtgatta agtcgctcat
     8401 ctgcaacttc cgtctggatt acgcccctat tgagaaacag tgggatttgc acttcgctga
     8461 ttactttgcg gaagatctca agctgctcgc cccgttagca aaagatgggc tggtggatgt
     8521 ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg ctgatccgca acatttgcat
     8581 gtgctttgat acctatctgc gccagaaagc gcggatgcag cagttctcac gggtgattta
     8641 aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa gcgccatcaa tgtgctgaga
     8701 acaagattgc ctgatgcgct acgcttatca ggcctacaaa acctgttgaa aatattgctt
     8761 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagagca cttcatcagc
     8821 aatctgaaac tcaactaaac agcccaatca ttgcggcaca cagcacggca gcaacggcag
     8881 tttgcggtgt gtggctgtca actgcgccac tcgataccag attaattatt gattccagca
     8941 taatgactct ccccgttttc cgggcaagat catactgaac ttatcggaac agtaaagcgt
     9001 aaaataccag caatttgcgc tcaataatca atctttacac acaagctgtg aatcactcca
     9061 tccccagctc ttttaactta cgcgtcaggg tgttgcggcc ccagccgagt agccgcgccg
     9121 cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt cagtaacgtc cgctccagct
     9181 ctggctgcgc ttcggaaagc agattttgat gaccggaacg cagcgctctg tctgcccact
     9241 gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga agtactctcc gcaaccgttg
     9301 attcaaacag ttcgccgggc aaatcctgaa tcaacacttc ctgcccggcg gccatcaccg
     9361 ttagccagcg gcaggtgttt tccagctggc gcacgttgcc tggccacgcc agacgcgtca
     9421 gagcagcttc ggtttccgga tgcagtaact tcgcttctac gcccagttcg cgcgcggcaa
     9481 cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg acgttcgcgc agcggcggca
     9541 gatgaacgcg gataacgttc aggcggtgga acagatcctc acggaactta ccttcctgca
     9601 ctcgctgttc gagattctgg tgagtggcag cgataatacg cacatccact ttcaccggcg
     9661 catagccgcc aacgcggtaa aactgaccgt ctgccagcac gcgcagcaaa cgcgtctgca
     9721 catccagcgg catatcacca atttcgtcga ggaataatgt accgccatcg gcctgttcaa
     9781 aacgcccctg acgaatggta ttcgcgccag taaacgcgcc tttctcgtgg ccaaacagtt
     9841 ctgattcgat caaatctttt gggatagctg ccatattcag cgcgataaac ggcgctttgg
     9901 cgcgcggact gtggcgatgc agggcatgag cgaccagttc tttaccggtg ccggattcgc
     9961 cgttaatcag cacgctaata gaagaacgcg aaagccgacc gataatacgg aacacgtcct
    10021 gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc gttaagctga atattacgcg
    10081 gctgctgctg ttcctggtaa tgactgatag cgcgctcaac cagcgccact gcttcgtcga
    10141 tatcaaacgg tttgggcaga taatcaaacg ccccttgttg ataggcgctg acggcagcat
    10201 ccagatcgga atgtgcggtc ataatgatga ccggaagcat tggatggcgc tgtttaatct
    10261 gcttgagcag cgccagcccg tccattcccg gcatacggat atctgaaagc agcacatccg
    10321 gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt ctcaaatgtc gtacaggtta
    10381 aacccgctcc agcgagcgca cgttcaagca cccaacggat ggaactatcg tcatcgacta
    10441 cccagactat ccctcgttgc ataaacgtca cctttatttc ctgataggca ggtaaaccga
    10501 gaactcggta tgacctggcc aactggtaaa ttcaattttg cctgaatgct gatcaatcaa
    10561 attacgggcg atggataagc caagcccggt gccaccttcg cggccgctga ccatcgggta
    10621 aaacagcgta tcctgcaaat gaggcggaat gcccggcccg ttatcttcca catcaatccg
    10681 cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga aacgcggtgc gggtacgcag
    10741 aatgatttca ccgccttccg gccccagcgc ctgtagcgca ttgcgcacaa tattcagcaa
    10801 gacctgttca atttgatccg ggtcgtgcgc cagttccggc aggctggggt cgtaatcacg
    10861 aatcaaccgc acgttgtccg gcagttccat cgacaccagc gtcaccacgc gttcagctac
    10921 tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc ggccccaaca gacggtcgac
    10981 cagatttcgc agccggtccg cctgttcgat aatcactttg gtatattcga gtagtgatgg
    11041 gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt aaacctccaa gcggattttt
    11101 aatctcatgt gccaggccgc gcactaaatc acgggcagca acctgctggg cgtgctgtag
    11161 ctgttcctga cttaagcggc gctggttatc catcggagcc atctccagca ggatcatgcc
    11221 gtccggcata cgctgggccg tcacagaaag gatatgcgag cgcccgtcga tgaccagcgt
    11281 cacttcgtta tcggtaaaac cttgccccgc ctccagactt tcttgcatca gctcgatatt
    11341 taatgagaag tagctcaaca gttccggtaa cggtgtacca aacaatttgc gggagctttg
    11401 ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc gccagattgt catcaattaa
    11461 caaaatactg ttaatcagcg agttgaggat ctgcccagca tcgggctgcg tgcctgttgc
    11521 cataaagcag tctcctgaac aggttgcacc attttagtgc attatagctt tttacggata
    11581 aaaagcgcga agcatcagag aattgacgga gaaaaaagcc catgcagaga tgggctgaaa
    11641 gtttccacgg caactaaatc ccggcgttgt tgcgccgggt agtacaactt caactattag
    11701 acgctgtagt acagctcaaa ctctaccgga tgcggagtca tacgcacgcg gtcatcttct
    11761 tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag tgaacacgcc accggctttc
    11821 aggaactcgc ggtccagatc cagttcgttc agtgcttctt ccagagagcc tgcaacctgt
    11881 gggatctctt tcgcttcttc tggcggcagg tcatacaggt ttttgtccat ggcttcgccc
    11941 ggatggatct tgttcttgat accatcaaga ccggccatca gcagggcagc aaagcacagg
    12001 tacgggttag ctgccggatc cgggaaacgt acttcgatac gacgtgcttt cggagaagaa
    12061 accaccggaa tacggataga cgcagaacgg ttacgcgcag agtaagccag cattaccggt
    12121 gcttcatagc ccgggaccag acgcttataa gagttggtgg tcgggtttgc cagggcgtta
    12181 atcgctttag cgtgtttgat tacgccgcca atgtagtaca gcgcctgctc agacagacct
    12241 gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag acagagacat gtggcagtgc
    12301 ataccggagc cgttatcacc gaacatcggt tttggcataa aggtcgcggt tttaccgaag
    12361 cggtgcgcta cgttgtgcac aacatatttg tagatctgaa tttcgtcagc ttttttggtc
    12421 atagtattga agcgggtagc cacttcgttc tgaccagcag tcgctacttc gtggtgatgg
    12481 gcttcaacca ccagacccat ctgttccatc accagacaca tttcagaacg aatatcctga
    12541 gccgagtcta ccggtggaac cgggaagtaa ccgcctttca ctgccggacg gtgacctttg
    12601 ttaccacctt cgtattgggt ggaggagttc catgcgcctt cgatatcgtc gatagcaacg
    12661 tgggaaccgg agatagatga tccgaaacgg atgtcatcga acaggaagaa ttcaggttct
    12721 ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca ggtaatcttc ggcgcgcttc
    12781 gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc caggttcaag gatgtcgcaa
    12841 cggataatca gggtggagtc ggcgaagaac gggtcaatca ctgcggtgga tgcgtctggc
    12901 atcagcacca tgtcggactc gttaatgcct ttccagccgc caatcgagga gccgtcaaac
    12961 attttgcctt cttcgaagaa ttcagcattc acctgatgag cagggatagt gacgtgctgt
    13021 tctttacctt tagtatcggt gaagcgcaaa tcaacaaact tcacttcgtg ctcgttcagc
    13081 atcgtcagta cgtgttcagc ggacatactt taactctcct ggattggtca tggtcgtcgt
    13141 ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa aaaagataaa gcgaaatctg
    13201 tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg caccatcgtg caaaagggct
    13261 gcaccacgat gtgaatgttg caccaatata gtgcttcaat ggaaacatta agcaccatgt
    13321 tggtgcaatg acctttggat aacccttttt atgctccgtg aaagcgatca caaagggact
    13381 ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt tgtagtgcga ttaatccgtg
    13441 tacaataacg cgctatttct aatgcctgag gcaaagttgt gatcgaaaaa ttgcgtaata
    13501 tcgccatcat cgcgcacgta gaccatggta aaaccaccct ggtagacaag ctgctccaac
    13561 aatccggtac gttcgactct cgtgccgaaa cccaagagcg cgtgatggac tccaacgatt
    13621 tggagaaaga gcgtgggatt accatcctcg cgaaaaacac cgctatcaaa tggaatgatt
    13681 accgtatcaa catcgttgat accccggggc acgccgactt cggtggtgaa gttgaacgtg
    13741 taatgtccat ggtagactca gtgctgctgg tggttgacgc atttgacggc ccgatgccgc
    13801 aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct gaagccgatt gttgttatca
    13861 acaaagttga ccgccctggc gcgcgtcctg attgggttgt ggatcaggta ttcgatctgt
    13921 tcgttaacct cgacgcgacc gacgagcagc tggacttccc gatcgtttac gcttctgcgc
    13981 tgaacggtat cgcgggtctg gaccacgaag atatggcgga agacatgacc ccgctgtacc
    14041 aggcgattgt tgaccacgtt cctgcgccgg acgttgacct tgacggtccg ttccagatgc
    14101 agatttctca gctcgattac aacagctatg ttggcgttat cggcattggc cgcatcaagc
    14161 gcggtaaagt gaagccgaac cagcaggtca ctatcatcga tagcgaaggc aaaacccgca
    14221 acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga acgtatcgaa accgatctgg
    14281 cggaagctgg cgatatcgtg gcgatcacgg gccttggcga actgaacatt tctgacaccg
    14341 tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc cgttgatgag ccgaccgttt
    14401 ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa agaaggtaag ttcgtaacgt
    14461 ctcgtcagat cctggatcgt ctgaacaaag aactggtaca caacgttgcg ctgcgcgtag
    14521 aagaaaccga agacgccgat gcgttccgcg tttctggtcg tggcgaactg cacctgtctg
    14581 ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc ggtatcccgt ccaaaagtaa
    14641 tcttccgtga aatcgacggt cgtaaacaag agccgtatga aaacgtgact ctggacgttg
    14701 aagaacagca tcagggttct gtaatgcagg cgctgggcga acgtaaaggc gacctgaaaa
    14761 acatgaatcc agacggtaaa ggccgcgtac gtctcgacta cgtgatccca agccgtggtc
    14821 tgattggctt ccgttctgag ttcatgacca tgacttccgg tactggtctg ctgtactcca
    14881 ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg tcagcgtcag aacggcgtac
    14941 tgatctctaa cggtcagggt aaagcggtcg cgttcgcact gttcggtctg caggaccgcg
    15001 gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg tcagattatc ggtattcaca
    15061 gccgctctaa cgacctgact gtaaactgcc tgaccggtaa gaaactgacc aacatgcgtg
    15121 cttccggtac tgacgaagct gttgttctgg ttccgcctat ccgcatgact ctggaacaag
    15181 ctctggagtt catcgatgat gacgaactgg tagaagtgac tccgacctct atccgtattc
    15241 gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa ccgcgcaccg aaagacgatt
    15301 aatttcgttt tttagttata aaaaacctgc catcgct
//
LOCUS       DAZOXR010000095        15179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZOXR010000095 DAZOXR010000000
VERSION     DAZOXR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.097"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHUNN_002567"
     CDS             <1..153
                     /locus_tag="ACHUNN_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0576911.1"
                     /translation="LRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
     gene            182..346
                     /locus_tag="ACHUNN_002568"
                     /pseudo
     CDS             182..346
                     /locus_tag="ACHUNN_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            complement(384..1445)
                     /locus_tag="ACHUNN_002569"
     CDS             complement(384..1445)
                     /locus_tag="ACHUNN_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0576912.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1658..3121
                     /gene="bepA"
                     /locus_tag="ACHUNN_002570"
     CDS             1658..3121
                     /gene="bepA"
                     /locus_tag="ACHUNN_002570"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0576913.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            3142..3501
                     /gene="arsC"
                     /locus_tag="ACHUNN_002571"
     CDS             3142..3501
                     /gene="arsC"
                     /locus_tag="ACHUNN_002571"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0576914.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3639..4340)
                     /locus_tag="ACHUNN_002572"
     CDS             complement(3639..4340)
                     /locus_tag="ACHUNN_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0576915.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="ACHUNN_002573"
     CDS             complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="ACHUNN_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0576916.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5810..6436)
                     /gene="upp"
                     /locus_tag="ACHUNN_002574"
     CDS             complement(5810..6436)
                     /gene="upp"
                     /locus_tag="ACHUNN_002574"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0576917.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6761..7798
                     /gene="purM"
                     /locus_tag="ACHUNN_002575"
     CDS             6761..7798
                     /gene="purM"
                     /locus_tag="ACHUNN_002575"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0576918.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7798..8436
                     /gene="purN"
                     /locus_tag="ACHUNN_002576"
     CDS             7798..8436
                     /gene="purN"
                     /locus_tag="ACHUNN_002576"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0576919.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8607..10673
                     /gene="ppk1"
                     /locus_tag="ACHUNN_002577"
     CDS             8607..10673
                     /gene="ppk1"
                     /locus_tag="ACHUNN_002577"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0576920.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10678..12219
                     /gene="ppx"
                     /locus_tag="ACHUNN_002578"
     CDS             10678..12219
                     /gene="ppx"
                     /locus_tag="ACHUNN_002578"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0576921.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12258..14501)
                     /gene="pdeF"
                     /locus_tag="ACHUNN_002579"
     CDS             complement(12258..14501)
                     /gene="pdeF"
                     /locus_tag="ACHUNN_002579"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0576922.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14683..14835)
                     /locus_tag="ACHUNN_002580"
     CDS             complement(14683..14835)
                     /locus_tag="ACHUNN_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576923.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14853..15044
                     /locus_tag="ACHUNN_002581"
     CDS             14853..15044
                     /locus_tag="ACHUNN_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0576924.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         4008 a         3877 c         3741 g         3553 t
ORIGIN      
        1 ctcagggatc cgctctccag ggcttactac acccgcaaaa tgagtcaggg aaaacgacac
       61 aatcaggcgc ttatcgccct ggcgagacga cgctgcgacg ttctgttcgc catgatgcgc
      121 gacgggactt tttatacccc gcaggggtca taacatgctt gacaacttaa taggggcacc
      181 ccccttctgg caactggcgc acagtagcgc agacaatttt ccggcactga cggtaagcca
      241 ttttattacc gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct
      301 ggtgagtatg tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc
      361 gaaacagttc gtcgggctga agattattct tgcgctattt gcccatcggg ccaggcgtgg
      421 atcacggctt tgatcagcgt cgccaatgga atagcgaaga atacgcccca gaatccccac
      481 aaaccaccga agatcaccac cgataaaata atcaccagcg gatgcaggtt aaccgcttcg
      541 gagaacaaca ccggtactaa cagattaccg tccagcgcct gaataatcag atacactgcg
      601 aagcagctcc agaattccgt gcctgcacca aactggaata gcgccacgcc aaccaccgga
      661 atggtcacca caaatgcgcc aatgtacgga ataagtaccg agaagccgac cagcaccgcc
      721 agcagcagcg aatagttcag cccaaagagc aagaacccca gccaagtggc gatccccacc
      781 acgatcatct ccagcacttt gccgcggata tagttggtga tttgttgatt catctccttc
      841 cacacctgtc ctgccagtcc acggttgcgc ggcagcaccc gacgaacggc gttcagcatc
      901 tgctctttgt ctttcagcag gaagaagacc atcaatggta ccagcaccag atagacggca
      961 atggtcagca aaccgaccag cgaggcgagg gaaattttca ccaccgaatc gcccatggtc
     1021 agcatccgac tgcgcatatt ttcggccatt gcatcaataa tgcccgcgtc cattaatgcc
     1081 ggatagcggc gcggcaacgt ggcggcaaag tcagaaagct tattgagcat ccccggcata
     1141 tcgcggatca agtagatgcc ctgttgccag gcgataggca gtaccacgaa cgccatcagt
     1201 agcaatatac cgacgaaaac caccaataca atcgacgtcg cccagcggcg ggagcagcca
     1261 atagattgca ggcgcacggt tggccattcc agcaaatagg ccagcacaat agccaccagc
     1321 aacggagcaa gcaggccact aaagaaaaag ataatgccaa atccggcaac taaaataacc
     1381 agcaaggcaa tcgcttccgg gtcgctaaaa cggcggcgat accattgcat caacatttcg
     1441 agcataaaga ccttccctga acctcaagag cgggattgcg atccgcaatt gtatcgaaat
     1501 gtcacaaaaa agacttcgct ttttatgacg gattcaggaa actgaaaagt catttgagtg
     1561 ggctaatctt cgccgttaca ctcaaaggcg gcgcggtggg aacaatattt cacagtatcg
     1621 gtcaaatgac tatcttcaga aatacaggat agaggttatg ttcaggcagt tgaaaaaaaa
     1681 cctggttgca accctcattg ctgctatgac cattggtcag gtagccccgg cgtttgccga
     1741 cagcgcagac accttgccgg atatgggaac ctccgcagga agcacgcttt ccattggtca
     1801 ggaaatgcag atgggcgact attatgtccg ccagctacgc ggcagcgcgc cgttaattaa
     1861 tgacccgctg ttaacgcaat atattaattc gctggggatg cgtctggttt cgcatgccaa
     1921 ttcggttaag acaccgtttc atttttttct gatcaacaac gacgaaatta acgcctttgc
     1981 tttctttggc ggcaacgtgg tgctgcactc tgccctgttc cgttattccg ataacgaaag
     2041 tcaactggct tcagttatgg cgcacgaaat ctcccacgtc acccaacgtc acctggcgcg
     2101 agcgatggaa gatcagcagc gcagcgcgcc gctgacctgg gtcggcgcgt taggttctat
     2161 tttactggcg atggccagtc cgcaggcggg gatggcggcg ctgaccggta cactggcggg
     2221 aacgcgtcag gggatgatca gtttcaccca gcaaaatgaa caggaagcgg accgcattgg
     2281 tattcaggtg ctgcaacgct cgggattcga tccgcaggcg atgccaacct tcctcgaaaa
     2341 attactcgat caggcgcgtt actcctcgcg cccgccggaa attttattga ctcacccgtt
     2401 gccggaaagt cgtctggcag atgcccgcaa ccgtgctaat cagatgcgcc cgatggtggt
     2461 gcagtcgtcg gaagatttct atctggcgaa agcgcgcaca ctggggatgt ataattccgg
     2521 acgtaaccag ctcaccagtg atttgctgga tgaatgggcg aaaggaaacg ttcgtcagca
     2581 acgagcggcg caatatggtc gtgctttaca ggcgatggaa gccaataaat acgacgaggc
     2641 gcgtaaaacg ctgcaaccgt tactggcggc agaacctggc aacgcatggt atctcgatct
     2701 ggctaccgat atcgatcttg ggcaaaacaa agccaatgag gcaatcaatc gcctgaaaaa
     2761 tgcccgtgat ttgcgcacca atccggtgtt gcagctcaac ctggcaaacg cttatctgca
     2821 aggcggtcaa ccacaagaag cggccaatat tctgaatcgc tacaccttta ataataaaga
     2881 tgacagcaac ggctgggatt tactggcaca ggcggaagcc gcgctaaata accgcgatca
     2941 ggagctggct gcgcgagcag aaggttatgc gctcgccgga cgactcgatc aggccatttc
     3001 gctgttgagt agcgccagtt cgcaggtgaa attaggcagc cagcaacaag cgcgttacga
     3061 tgcgcgcatc gaccagttgc gccagctgca ggaacgcttt aagccttata ccaagatgta
     3121 attcgcatta aaggaagaac aatgaccaaa caggtaaaaa tctaccataa cccacgctgt
     3181 tcaaagagcc gggaaacgct gaacctgctg aaaaaaaacg gcgtggagcc agaagtggta
     3241 ctctaccttg agacacccgc cgatgcggca acgctgcgcg atttgctgaa aatgctgggg
     3301 atgaatagcg cccgtgaatt gatgcgccag aaagaggatc tttataaaga actgaacctg
     3361 gctgacagtt cgcttagcga agaggcgctg attcaggcaa tggtggataa tccgaagctg
     3421 atggagcgcc cgattgtggt cgcgaatggc aaagcgcgga ttggtcgtcc accggagcag
     3481 gtactggaaa tcgtgggtta agatgtgccc catcgctagt tgaagcacat tgccggatgc
     3541 gacgcttgcc atgtcttatc cggcctacga atactatgga ttaacttcgc gtagttcgga
     3601 taaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc
     3661 acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac
     3721 gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct
     3781 tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca
     3841 tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gtcgcgacgc gagatccggt
     3901 aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaatcg tgttttgccc
     3961 gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca
     4021 atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc
     4081 ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac
     4141 aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga
     4201 tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcagccag taaagaggag
     4261 ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag
     4321 agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt
     4381 accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt
     4441 gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactaat
     4501 cagtttgaag atcaaactta agccgatacc gacgatggtc gccagcgcca tacctttcag
     4561 ctccgccgcg ccaatgttta ccttcgcgcc actgacaccg atgatcaaaa tcacggaagt
     4621 caggatcagg ttctgtgctt tgttgtaatc cacttttgat tcgatcaaaa cacgaatacc
     4681 ggaagcaccg atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat
     4741 catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc
     4801 cccaccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc
     4861 gtaagtggta tttggcgtag agccaaagaa gccggagatc acagtcgaca ggccgttagc
     4921 aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt
     4981 tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag
     5041 aatggcaaac cactcgaagc gcggcgtata gagggttggc agcgcaaacc agtgagcatt
     5101 aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac
     5161 gccaattaaa atcgggataa ttgccaggaa accacggaac agcacggaac ctaaaaccgt
     5221 gaccgccagg gtggtaatag agatgatgat ggttttggag tctggcgttt gcccttcagc
     5281 cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac
     5341 gattgcgccc attgccgcag gtggaaacag cacgtccagc cagccggtcc ccgctttctt
     5401 cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac
     5461 ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag
     5521 ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa
     5581 taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa
     5641 catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact
     5701 caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt
     5761 actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac
     5821 caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc
     5881 cctggtcaat cgatgcggta tacagttcga cgtccgggtg cgctttttcc agcgcagcga
     5941 taccttctgg cgcagctacc agcaccagaa ctttgatgct gctgcagccc gcttttttca
     6001 gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg
     6061 ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt
     6121 cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacattt tccagcacac
     6181 cgtccatcat ccccagaccg gcacgcagaa ttggcacaac ggtaattttc ttacctttga
     6241 tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt
     6301 cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct
     6361 tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga
     6421 cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgacggat
     6481 tatacctcct ttcttcaagg cggcaatatt cttttcgttg actttagtca aaatgataac
     6541 ggtttgagat aaagttattt tatattcaaa tggttatgaa agaagattat tccatccgaa
     6601 aactaacctt tgccctggca caagtcttct ttcgccgcgc gtctggggaa aagacgtgca
     6661 aaaaggttgt gcaaagcagt ctcgaaaacg tttgctttcc ctgttagaat tgcgccgaat
     6721 tttatttttc taccgcaagt aacgcgtggg gacccaagca gtgaccgata aaacctctct
     6781 tagctacaaa gatgccggtg ttgatattga cgcgggtaat gctctggttg gaagaatcaa
     6841 aggcgtagtg aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc
     6901 gctgtgtgca ttgccgcaaa aatatcgtga acccgtgctg gtttccggca ctgacggcgt
     6961 aggtaccaag ctgcgtctgg caatggactt aaaacgtcac gacaccattg gtattgatct
     7021 ggtcgccatg tgcgttaatg acctggtggt gcaaggtgca gaaccgctgt ttttcctcga
     7081 ctattacgca accggaaaac tggatgttga taccgcttca gcggtgatca gcggtatcgc
     7141 ggaaggatgt ctgcaatcgg gctgttcact ggtgggtggc gaaacggcag aaatgccggg
     7201 gatgtatcac ggtgaggatt acgatgtcgc gggtttctgc gttggcgtgg tagaaaaatc
     7261 agaaatcatc gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag
     7321 cggtccgcac tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga
     7381 tccgcaaacc accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg
     7441 catttacgtg aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca
     7501 cctgaccggc ggcggcttct gggaaaacat tccgcgcgta ttgccagata atactcaggc
     7561 agtgattgat gaatcttcct ggcagtggcc ggaagtgttc aactggctgc aaacggctgg
     7621 taacgttgag cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc
     7681 cctgcctgct ccggaagtgg acaaagccct cgccctgcta aatgccaacg gtgaaaacgc
     7741 gtggaaaatc ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataatg
     7801 aatattgtgg tgcttatttc cggcaacgga agtaatttac aggcaattat tgacgcctgt
     7861 aaaaccaaca aaattaaagg caccatacgg gcagttttca gcaataaggc cgacgcgttc
     7921 ggccttgaac gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt
     7981 gacagtcgtg aagcctatga ccgggagttg attcatgaaa tcgacatgta cgcacccgat
     8041 gtggtcgtgc tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc
     8101 gggcgtttgc tgaacattca cccttctctg ctgccgaaat atcccggatt acacacccat
     8161 cgtcaggcgc tggaaaatgg tgatgaagag cacggtacat cggtgcattt cgtcaccgat
     8221 gaactggacg gtggcccggt tattttacag gcgaaagtcc cggtatttgc tggtgatacg
     8281 gaagatgaca tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc actggtgatt
     8341 agctggtttg ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa
     8401 cgtctgccgc cgcagggcta cgctgccgac gagtaatgcc ccgtagttaa agcgccagct
     8461 ctgccgctgg cgtttttcaa ttcacctgta aatcgcaagc tccagcagtt tttttccccc
     8521 ttttctggca tagttggaca tctgccaata ttgctcgcca taatatccag gcagtgtccc
     8581 gtgaataaaa cggagtaaaa gtggtaatgg gtcaggaaaa gctatacatc gaaaaagagc
     8641 tcagttggtt atcgttcaat gaacgcgtgc ttcaggaagc ggcggacaaa tctaacccgc
     8701 tgattgaaag gatgcgtttc ctggggatct attccaataa ccttgatgag ttctataaag
     8761 tccgcttcgc tgaactgaag cgacgcatca ttattagcga agaacaaggc tccaactctc
     8821 attcccgcca tttactgggc aaaattcagt cccgggtgct gaaagccgat caggaattcg
     8881 acggcctcta caacgagctg ctgctggaga tggcgcgtaa ccagatcttc ctgattaatg
     8941 aacgccagct ctccgtcaat caacaaaact ggctgcgtca ttattttaag cagtatctgc
     9001 gtcagcacat tacgccgatt ttaatcaatc ctgacactga cttagtgcag ttcctgaaag
     9061 atgattacac ctatctggcg gtggaaatta tccgtggcga taccatccgt tacgcgctgc
     9121 tggagatccc atcagataaa gtgccgcgct ttgtgaattt accgccagaa gcaccgcgtc
     9181 gacgcaagcc gatgattctt ctggataaca ttctgcgtta ctgccttgat gatattttca
     9241 aaggcttctt tgattatgac gcgctgaatg cctattcaat gaagatgacc cgcgatgccg
     9301 aatacgattt agtgcatgag atggaagcca gcctgatgga gttgatgtct tccagtctca
     9361 agcagcgttt aactgctgag ccggtgcgtt ttgtttatca gcgcgatatg cccaatgcgc
     9421 tggttgaagt gttacgcgaa aaactgacta tttcccgcta cgactccatc gtccccggcg
     9481 gtcgttatca taattttaaa gactttatta atttccccaa tgtcggcaaa gccaatctgg
     9541 tgaacaaacc actgccgcgt ttacgccata tttggtttga taaagcccag ttccgcaatg
     9601 gttttgatgc cattcgcgaa cgcgatgtgt tgctctatta tccttatcac acctttgagc
     9661 atgtgctgga actgctgcgt caggcttcgt tcgacccgag cgtactggcg attaaaatta
     9721 acatttaccg tgtggcaaaa gattcacgta tcatcgactc gatgatccac gctgcgcaca
     9781 acggcaagaa agtgaccgtg gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca
     9841 ttcactgggc gaagcgcctg accgaagcag gcgtgcacgt tatcttctct gcgccggggc
     9901 tgaaaattca cgccaaactg ttcctgattt cacgtaaaga aaacggtgaa gtggtccgtt
     9961 acgcacacat cgggaccggg aactttaacg aaaaaaccgc gcgtctttat actgactatt
    10021 cgttgctgac cgcagatgcg cgtatcacca acgaagtacg gcgggtattt aactttattg
    10081 aaaacccata ccgcccggtg acatttgatt atttaatggt gtcaccgcaa aactctcgcc
    10141 gtctgttata tgaaatggta gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagtg
    10201 gtatcaccct gaagctaaat aaccttgtcg ataaaggcct ggttgatcgt ctgtatgtgg
    10261 cctccagctc cggcgtaccg gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca
    10321 atctggaagg cattagcgac aacattcgtg ccatcagtat tgttgaccgt taccttgaac
    10381 atgaccgggt ttatattttt gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg
    10441 actggatgac gcgcaatatt gattatcgta ttgaagtggc gacaccgctg ctcgatccgc
    10501 gcctgaagca gcgggtgctg gacatcatcg acatattgtt cagcgatacg gtcaaagctc
    10561 gttatatcga taaagaactc agtaatcgct acgttccccg cggcaatcgc cgcaaagtac
    10621 gggcgcagtt ggcgatttat gactacatca aatcactcga acaacctgaa taaccctatg
    10681 ccaatacacg ataaatcccc tcgtccgcag gagtttgctg cggtcgatct tggttcaaac
    10741 agttttcaca tggtcatagc ccgtgtggta gatggtgcca tgcagattat tggccgcctg
    10801 aaacagcggg tgcatctggc ggacggcctg gggccagata atatgttgag tgaagaggca
    10861 atgacgcgcg gtttgaactg tctgtcgctg tttgccgaac ggctacaagg gttttctcct
    10921 gccagcgtct gtatagtcgg cacccatacg ctgcgtcagg cgctgaacgc cactgacttt
    10981 ctgaaacgcg cggaaaaggt cattccctac ccgattgaaa ttatttccgg taatgaagaa
    11041 gcccgtctga tttttatggg cgtggaacat acccaaccgg agaaaggtcg caaactggtt
    11101 attgatattg gcggcggatc tacggaactg gtgattggtg aaaatttcga accgattctc
    11161 gttgaaagcc gccggatggg ttgtgtcagc tttgcccagc tttacttccc tggcggggtc
    11221 atcaataaag agaattttca gcgcgctcgt atggcggcag cacaaaaact ggaaacttta
    11281 acctggcaat tccgtattca gggctggaac gtggcaatgg gcgcttccgg taccataaaa
    11341 gccgcccatg aagtgttaat ggaaatgggc gagaaagacg ggataattac cccggaacgt
    11401 ctggaaaaac tggtaaaaga agttttacgt caccgtaatt tcgcatcgct gagtttaccg
    11461 ggtctttccg aagagcggaa aacagtcttc gttcctggac tggcgatttt atgcggtgtg
    11521 tttgatgctt tagccatccg tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta
    11581 ctgtacgaaa tggaaggacg tttccgtcat caggatgtgc gtagtcgcac cgccagcagc
    11641 ctcgccaacc agtatcacat cgacagcgaa caggcccgac gggtgctgga taccactatg
    11701 caaatgtacg aacagtggcg ggaacagcaa ccgaagctgg cgcatccgca actggaggcg
    11761 ctactgcgat gggccgccat gctgcatgag gtcgggttga atatcaacca cagcggtttg
    11821 catcgccact ccgcttatat tctgcaaaac agtgacttgc cgggttttaa tcaggaacag
    11881 cagctgatga tggcgacact ggtgcgctat caccgtaaag cgattaaact cgacgatctg
    11941 ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac tgattcagct attgcgcctt
    12001 ggcgtattac tcaacaatca acgtcaggca accaccacac cgccaacatt gacactgatt
    12061 actgatgaca gtcactggac actgcgtttc ccgcatgact ggtttagtca gaatgcgttg
    12121 gtactgcttg atctggaaaa ggagcaagaa tactgggaag gcgtggctgg ctggcggttg
    12181 aaaattgaag aagaaagtac acctgaaatc gccgcttaat gctttgcggg ccgacgagaa
    12241 atgtcggccc gcattattca ggcactttcg cgaatgggtt cgatttcatt caacgtatca
    12301 attaacggct gcggcttacc aataagataa ccctgcatat aatcgatccc caaagagagc
    12361 accgcctcgc ggatctcttc gttttcaacg tactctgcca ctacccgcat tttcttcatt
    12421 cgcgccagat ggcaaatcga cgccacaatc tgataatcca gactatttga cacaatattg
    12481 cggataaagc tgccgtcaat tttaagcaga tcggcattca catttttaag ccgtgcatag
    12541 ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc agcctaattc ctgaagatgc
    12601 tgcaaggtga tttgcgcctg cttaacattg gtcagagcat tactttcggt gacttcaaaa
    12661 ataagttgcc acgcttcaat ctgatattta gccagcaact gactgacttc aacaggaaaa
    12721 cgagcctgac ataccgaggt tggagacaga ttaatagcaa aacggtgagc gggcatcttc
    12781 gctctgtttt cagccataaa ttgcagcgta tgctcaatga cccacatgtc gatactcgac
    12841 gataaaccaa attcgtgcgc gaccggcaag aagctatcgg ggacgatcag ttcatcattc
    12901 tcacctttca tgcgcagaag aatttcatgg taaacatcac cacgcatacc ggtaatcggc
    12961 tgggccatca ggaaaaaatg gttgtgttcc agcgcctgct gtagtcgatt catcatcgcg
    13021 actttatctt tcaattcgcg ttgcaaatac attgccccgc gacgctgcat attttccggg
    13081 gcgttggtca cgatggaaag ttcggcgacc gtatttagct ctcccagcag caggtagata
    13141 tgattcactg gcgagcgcac atagcagtaa ctgacgccaa tctgcggttg catcggcatc
    13201 ccatcccaaa agaaacgaaa ttgcttgaga tgactatcca gtgcggtaat gcgctcctgg
    13261 tgcgattctg tattcagtcg cagcgcgaga tcgttacccg aaagctgata aacatcttca
    13321 cccggttcca gcaagggtga cagccagtga gaaagttttt gcttgtattg aatccgcagc
    13381 atgatgccat agttcttaac cagcatttcc atgccaggga tgcgtaaata acaaagcgca
    13441 gaccagggag catcacgcaa cgcgcgattc agggcgcgaa catttggcag atgaaccacc
    13501 gggtccatat acgcaagccg ctgaatgcgt ctgactaccg ctctctgacg ggttgccagt
    13561 acagccatat aattgacaat aaaagagaat accagataac tggaggaggt tatggtcagc
    13621 tgcgtggtat agccaggata aatgggaatg taattttgat agctgtggat gctgatcatc
    13681 aacacgaccg cccagagcag cgaaatcagc ttataaccat agcgcatcgc tccccacatc
    13741 atcaggggca gcaataatga caaggtataa ttggtgctaa aaattgtgct tttttcattt
    13801 aacggcatgc ataacagcaa caataaagca cctaatgcca gtagccagag cgcgaactct
    13861 tttttggtga ctttggcatc aacctgctgt tttaattgcg aataatagct acgtaaataa
    13921 aatggatttc gcactacccg aatgatgaag tagcacagcg ggacaccgat cagattaccc
    13981 accagtaagg cctgataatt gattaaagtc ccgaggttaa aaggcatgac accgaccaga
    14041 ttttctctgc tcgccagtaa tcctacaaat gcagcaaact ggaaaagtat cagaaacagc
    14101 gttgcaggaa acacaatctg ccagaatata cgctgggaaa ttaaacgggt atcaccatgt
    14161 gaaacgttgt tacgccgggg agtaaagacc ctgtaaccgc cccaacagag aatgataatg
    14221 ataataaaat tagccgttaa tgatagcgtt tcgtaaaaac ccagctctgc atatttgcgg
    14281 acaaaaatcc ctaaggcgat ccctggcaat gccgcccagc tgaaaaacat catcatgctg
    14341 atcatcagtg ccagaggcag ataaaagaga aaaacctcac cggaagaaat atgcgcgaaa
    14401 gtattaatgt gggcgaaaat gggcaagatt aaagaaggca ggaacagcgg aagcccccac
    14461 catttatcac gcatttttat ataagttgca ttcagtttca tagatgctca gcagaatccc
    14521 ccacatcctg aaggaggtgt attcagacag gcatcccacc tgacttcgaa tgatgattat
    14581 tcatcactat agagagcatt gattctaagt gtcatatgaa agttccaatt gatatatatc
    14641 aaacaaaata accctgatta atgaattatt acgtttatca tgttaattca tcattattac
    14701 atcatcattg taaataatta aattaacttc cataacatta aaatatgtat ccactgacgt
    14761 ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc
    14821 cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac
    14881 gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct
    14941 ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag
    15001 ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttcttcac
    15061 tttcacagaa cataacggca cttcgctgtc ggatgctttt gctctttggg attatcaaag
    15121 cggcagatat tctttcatct taaattttac gtctttgtcc tgactgatgt ttatcctgt
//
LOCUS       DAZOXR010000096        14965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZOXR010000096 DAZOXR010000000
VERSION     DAZOXR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.098"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUNN_002582"
     CDS             <1..196
                     /locus_tag="ACHUNN_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576925.1"
                     /translation="KAILTDGLFALAQKKMPDATLRVLYSHICQIQQRIRFPQLAHFH
                     TCPPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUNN_002583"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUNN_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0576926.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="ACHUNN_002584"
     CDS             complement(1608..1931)
                     /locus_tag="ACHUNN_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0576927.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUNN_002585"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUNN_002585"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0576928.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUNN_002586"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUNN_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0576929.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUNN_002587"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUNN_002587"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0576930.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUNN_002588"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUNN_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0576931.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="ACHUNN_002589"
     CDS             7531..8568
                     /locus_tag="ACHUNN_002589"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0576932.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUNN_002590"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUNN_002590"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0576933.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUNN_002591"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUNN_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0576934.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUNN_002592"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUNN_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0576935.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="ACHUNN_002593"
     CDS             10739..11620
                     /locus_tag="ACHUNN_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0576936.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUNN_002594"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUNN_002594"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0576937.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUNN_002595"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUNN_002595"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0576938.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="ACHUNN_002596"
     CDS             complement(14017..>14965)
                     /locus_tag="ACHUNN_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR0576939.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3785 c         3921 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgccc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAZOXR010000097        14788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZOXR010000097 DAZOXR010000000
VERSION     DAZOXR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.099"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHUNN_002597"
     CDS             complement(<1..181)
                     /locus_tag="ACHUNN_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576940.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFI"
     gene            259..1353
                     /gene="ddlA"
                     /locus_tag="ACHUNN_002598"
     CDS             259..1353
                     /gene="ddlA"
                     /locus_tag="ACHUNN_002598"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0576941.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(1377..1589)
                     /locus_tag="ACHUNN_002599"
     CDS             complement(1377..1589)
                     /locus_tag="ACHUNN_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0576942.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1849..2157
                     /locus_tag="ACHUNN_002600"
     CDS             1849..2157
                     /locus_tag="ACHUNN_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0576943.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2216..3310)
                     /gene="yaiW"
                     /locus_tag="ACHUNN_002601"
     CDS             complement(2216..3310)
                     /gene="yaiW"
                     /locus_tag="ACHUNN_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR0576944.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3323..4543)
                     /gene="sbmA"
                     /locus_tag="ACHUNN_002602"
     CDS             complement(3323..4543)
                     /gene="sbmA"
                     /locus_tag="ACHUNN_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0576945.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4895..6052
                     /gene="ampH"
                     /locus_tag="ACHUNN_002603"
     CDS             4895..6052
                     /gene="ampH"
                     /locus_tag="ACHUNN_002603"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0576946.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6053..6676)
                     /gene="iprA"
                     /locus_tag="ACHUNN_002604"
     CDS             complement(6053..6676)
                     /gene="iprA"
                     /locus_tag="ACHUNN_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR0576947.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6764..9646)
                     /locus_tag="ACHUNN_002605"
     CDS             complement(6764..9646)
                     /locus_tag="ACHUNN_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0576948.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITNGGVLDVNTNMNDISSVFAT
                     DTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMNSD
                     GHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYIDD
                     TKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQ
                     DTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNA
                     KWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSA
                     TMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMK
                     VDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTE
                     GSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            10172..11146
                     /gene="hemB"
                     /locus_tag="ACHUNN_002606"
     CDS             10172..11146
                     /gene="hemB"
                     /locus_tag="ACHUNN_002606"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0576949.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11252..12103)
                     /gene="tauD"
                     /locus_tag="ACHUNN_002607"
     CDS             complement(11252..12103)
                     /gene="tauD"
                     /locus_tag="ACHUNN_002607"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0576950.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(12100..12927)
                     /gene="tauC"
                     /locus_tag="ACHUNN_002608"
     CDS             complement(12100..12927)
                     /gene="tauC"
                     /locus_tag="ACHUNN_002608"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0576951.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(12924..13691)
                     /gene="tauB"
                     /locus_tag="ACHUNN_002609"
     CDS             complement(12924..13691)
                     /gene="tauB"
                     /locus_tag="ACHUNN_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0576952.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13704..14666)
                     /gene="tauA"
                     /locus_tag="ACHUNN_002610"
     CDS             complement(13704..14666)
                     /gene="tauA"
                     /locus_tag="ACHUNN_002610"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0576953.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         3716 a         3838 c         3603 g         3631 t
ORIGIN      
        1 ttatgaaata tattctcttc gttaacttat aattaagaga aaaaaactca ccgcattgcc
       61 atttaagcca ggaataaatt agcttcccgt tcatcatttt tgtgatctcg cgcacatctt
      121 tgagataacg cactttcttc accagtacgt tgcacgcttt gtcagcagaa gattgcaaca
      181 atctttcaga aggccgcgaa tcccccgttt tgtcgtggta atgtatgcac tttgtgttag
      241 acgggatagg tttttaagat ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca
      301 gcggaacatg aagtgtctct gcaatcggca aaaaacattg tcgatgccat tgataaaagt
      361 cgcttcgacg ttgtgctgct gggcattgat aaacaagggc aatggcacgt cagcgatgcc
      421 agcaattatc tgctaaatgc agacgatcct gcccatattg cgttgcgccc ttcggcgacc
      481 agccttgcgc aggtgccagg taaacatgag catcagctta tcgacgcgca aaacggtcag
      541 ccgttgccga cggtggatgt cattttcccg attgtccacg gtacgctggg cgaagatggt
      601 tccttgcagg gaatgctgcg ggtcgccaat ttaccgtttg taggttctga tgttctggct
      661 tcagcagcct gtatggataa agatgtcacc aaacgtctgc tacgcgatgc cgggctgaac
      721 attgcgccat ttattaccct gacgcgcgct aatcgtcaca acatcagttt tgccgaagtg
      781 gagtctaaac tggggttacc actgtttgta aaaccggcta atcagggctc ttctgttggt
      841 gtcagcaaag taaccagtga agaacagtac gcaattgccg tcgatctggc gttcgagttc
      901 gatcataaag tgatcgttga gcaagggatc aaaggtcgtg agatcgaatg cgcagttctg
      961 ggcaacgaca atccgcaagc cagcacctgt ggcgagatcg tactcaccag cgatttctat
     1021 gcctacgaca ccaagtacat tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt
     1081 gcaccagaga tcaacgataa gatccgggcg attgccgttc aggcttatca gacgttggga
     1141 tgcgcaggca tggcgcgtgt agacgtgttt ttaaccccag agaacgaagt ggtgatcaac
     1201 gagatcaaca cactgcctgg cttcaccaac atcagtatgt atccgaagct gtggcaagcc
     1261 agcggtctgg gttacaccga tctgatcaca cgtttgattg agctggcgct gaagcgtcac
     1321 gctgcggata acgcattgaa agccacaatg taatatttta acgcccgata atattcttat
     1381 tgggcgtttt cagtttcttc atcgcgacga cgaataatca accccgccag ccagaaacta
     1441 atcacccacg tcaccagacc gacggcatag gtctgccaac cttttgcttc aaatcccagt
     1501 aaccccacca cgccattaag aatgaatata aggccaatgg cgaacgcata gtagtgccag
     1561 tcacggcgga tttttacagg cagattcatg gcagctccgt taacgaaaaa caacatcctt
     1621 gcatattctg taacaaccgt ctgtggtgta gacaacattt tcgtaattgt taataaattt
     1681 aacttaaggt tgccattttg taactcacaa cgaagattca cgcttcggga atattcccaa
     1741 catcaattta ccatgattct gatattgaca atctaaatga cctgtcagac ttgccataag
     1801 ccgctggtat cacaccagaa gagaagcaga tgacttccgg aggtttttat ggctgatttc
     1861 accctgtcaa aatcgctgtt tagcggaaaa tatcgcaatg cctcttcaac gcctggcaac
     1921 attgcctatg cgttgtttgt gctgttttgc ttttgggctg gggcgcaatt gctgaacctg
     1981 ttagtgcatg cgcccggcgt ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca
     2041 cgggtggaaa ttggtttagg tgtcggcacc attttcgggc tgatcccgtt tttagtaggc
     2101 tgcctcattt ttgcagtggt ggcgctatgg ctgcactggc gacatcgccg ccagtaagat
     2161 ttacgttgcc agatacggcc taaaacgcct tatctggcat gacattcctg tttgatcact
     2221 gtttcataca acgcgcccgc cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcaggt
     2281 tgcgggtgat tttcgggctt tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt
     2341 tacccgtttt cgcttcggca agttgataaa ctttcttgta cagcgccgtc tcctcaaaag
     2401 agaagctgtc acctttctct aactgacggc gaatttcgct gtcgctcatc cccagttttc
     2461 ccgccagttt gcgcgttgcc agttcggttt tcccgggttc tttactgtcg taacgaatta
     2521 aatcaccgtc cagtgccagc ttcacgccgc tggccttact gactgcgttc tgaaacgcgg
     2581 cattacggct ggcgtaccag ccagcgttaa aatcagcaaa gcggtatatc ggtgcgctat
     2641 agctggcggg ataattcagt aaatggtaag taccaaacca caacccgccg cggcgactga
     2701 agacttcctg acgaactgta ccgtccattt tccacggata ccctttggta tgctgttcgg
     2761 caaaagcaat gctgacctgc atcggcccac cggtgcgcac cggattaagc gaaccaaaca
     2821 gcgtctgccc cattggtacc atgctgatca gatcgtcaaa aatcgcgctc aattgctttt
     2881 ctgtacgcac cgaatctaac cgttcgctat aacttttgcc gtttggcgat ttgattttta
     2941 gcgcggtatg aaccagaaaa gcaggaatgt gcatccgttc agcacgacgg tcaatctctt
     3001 gccaggcgat cttgcttaag cccggaacgg ccggatctgc ctgataattt gactcctgct
     3061 gcgccaccgc cagcaccgag cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa
     3121 aggtgatggc gagatctttc gcccaggcat cgcggtcttt cacgctcgca ggcatcttct
     3181 gccgcacgac gctcgctaca tctatcgctt tttcgccctt tttcaacggt tgcggagcct
     3241 gactactaca ccccgctaaa accagaaccg ccaacaatga cagcgaagat agatgattga
     3301 cgcgcgacat tacttctcct ttttagctca aggtatgggt tacttcctga attttgtcac
     3361 catccagctc atgttcaaag ctgcgcagac gtttgtagat agacatcaac tcaaccagtg
     3421 tggtccatga gttaatcagg tactggaaag caccgcgaac ctgaccaaaa acgttggtaa
     3481 tctgcgtcat caggccgagc gtaatcgtac cggcaacaat tgacggaaac agcaagaaca
     3541 aaccgaaaac gttatcgacc tgcaaataga gaatgcgggc gatgttgaaa tacatatagt
     3601 gaaaatagag gcgaaaatag tttttccgta cggcgctaaa cagctcgcgt accgtaggcg
     3661 gcgtcgcgcg cgtggcatcg tcttcaccat aaaccagctc tttacggtag gcggcctcta
     3721 ctcgctggtt tttaaactcc agccccggca gtttgatccc taccactgcc agccatccgg
     3781 tccccatcag cgaccagacg atagcggcaa tcaccagacc atacggaatg tgcccgataa
     3841 tcggcagctc cggcacatgc gcggagagcg ttaccagcac cggcaggaag gcgatcaacg
     3901 tcatgatggc gttgataaaa ctgacgccca tattctccag cgttgaagca aaacgcatgg
     3961 tgtcttcctg cacacgctgt gcggcacctt cgatatgacg cagttgttgc cagttcgcca
     4021 tgtaatattc gttcatcgcc gtacgccagc ggaacacata gtgactgaca aagaagttgt
     4081 tcagtacact gatcaccacc gcgatcagcg caatccccag aaagacgccc acttcgcggt
     4141 aaaattgttc gatagtgact ttatgcggcg aacttagcgc ggtttgaatc agatcataga
     4201 acggtgcata ccaggcgttg acggcgaccc cgacttccac caaaaaccag gtgacgaaga
     4261 tgatcagtgc agtaccgagt attgaccagt attgccaacg gtgcgggctg tagataaacc
     4321 agaaaaatgc aaaaagtcct acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca
     4381 acgaccagaa acgcgcggcg ctaatcggga tctgcccgga agcgccggtg atacgcgcca
     4441 cccagtctcc cccaccggct tgccagaaga taacggcaat caatgcccaa acaaaggccg
     4501 agagaaaaaa cgctcccggc tttgggaaaa aagacttaaa catatgcact cctgctaact
     4561 tcttatcgtt ttaacaactg ctgtgtatta accgcatgac cactacaaag cgtcagccaa
     4621 atgaaaccgc ccattgtgac ccatagcaag gcgcttagtt cgccagcctg accataagaa
     4681 ttgtttcgac ggatttcaca aaccaaagat tgtttacata agcggattaa cacgcgcagg
     4741 atcacaaatg caggcattgg tgagcaggaa aaagatcgtg tttacgcgcc gttacgtttt
     4801 taacactatt attgcactaa ttcgcccttt gcaatccatc aatgagtagt ataaatacgc
     4861 tcagttacct tcattcaatc tatggacacc accgttgaaa cgtagtctgc ttttttctgc
     4921 cgtgctgtgt gcggcgtcat tgacctccgt ccatgcggcg cagccgatca ctgaaccgga
     4981 gtttgcctct gatattgtcg atcgttatgc cgatcatatt ttttacggca gcggtgcaac
     5041 ggggatggcg ctggttgtta tcgacggtaa ccagcgcgtc tttcgtagtt atggcgaaac
     5101 gcgacctggt aataacgttc gcccgcagct ggattcggtc gtgcgtattg cttccctcac
     5161 caaactaatg accagtgaaa tgctggtgaa attgctcgac caggggaccg taaagttaaa
     5221 cgatccgcta agtaaatatg ctccgccagg cgcacgcgtg ccgacctaca atgggacgcc
     5281 cattacccta gtgaatctgg caacccatac cagcgccctg ccccgtgaac agcccggtgg
     5341 cgcggcacat cgtccggtgt ttgtctggcc aacgcgcgag caacgctgga aatacctttc
     5401 tacggcgaag ctgaaagccg cgccaggcag tcaggcggcg tactctaacc ttgcgttcga
     5461 cctgctggca gatgcactgg cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga
     5521 gcagattacc cgtccattgg ggatgaaaga caccacctac accccctcac cggatcagtg
     5581 ccgccgttta atggttgctg agcgcggtgc cagtccgtgc aataacacgc tggcggcaat
     5641 tggcagcggc ggcgtctatt ccacgcctgg cgatatgatg cgctggatgc agcagtattt
     5701 gtcatctgat ttttatcagc gcagtaacca ggcagatcgc atgcagacgt tgatttatca
     5761 gcgcgcgcag tttacgaaag tgattggcat ggatgtcccc ggcaaagcgg atgcgcttgg
     5821 tttaggctgg gtatatatgg ccccgaaaga gggtcgtccg gggattattc agaagacagg
     5881 cggtggtggc ggtttcatta cctatatggc gatgatcccg cagaagaata ttggcgcgtt
     5941 tgtggtggtc actcgttcgc cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga
     6001 tctggtgacc gagctaagcg ggaataaacc gttggttatc cccgcatcct gattaatact
     6061 ctgaaggcaa acggttgata gcgaccagtt tgcctttatt catttcgata tagccgcctt
     6121 tacgcaaagc agcgagaact tctgcgacga cagaacgcga tatgcgtgta cgttgatgga
     6181 tatagttcat cacaccaata cgtgatcgca attcttcatt ccagtcaatc attgataata
     6241 atgttgcgcg gatttgttcg taggaattat gcccaatgag ctgcacgtcg cgtaattcca
     6301 gaattctatt ttgccaagct aaccagtaaa aagcgtctcg ccagagttga ttttgttcaa
     6361 taagcgtaat ggtttgtttg gctggtagat gatatcccgt acaatttcct tctgatatta
     6421 atttgtatgg tatatcgttt ttcattaaac catcagccag ccccataata taaggggcct
     6481 gggtaatacc gataagtacg ttttcttctc tacgcagaga gataactccc tctaaaataa
     6541 cgaaagtatc ttcgttattg acatcactgg aaaatataac ttgcttttca ttattaaact
     6601 cgaagcgcgt accgtatctg gacaaacatt tatcgagctt accaaattcc tgaagaggtt
     6661 taactacaga taacatttgc gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca
     6721 ttatttagat taaattacca gtatttcttc ggagtgaaga atattaccag gtatatttaa
     6781 cacccacgtt cgcggaccag tcttgatcta cgtcaccacc accgaggtag ttagcatcgg
     6841 tataggcgct gaagttcttg gtgaagctga actgagtacc cagaccaaca cgtaccgcag
     6901 acccttcagt accgttatcg atggaatcac cgttcacatc gttatcgttg ttagagtcgt
     6961 cgtagacgta agccagtttg aagtacggag tcagagcctg atcttcgctg taggtgaagg
     7021 tataacctgc atctacaccc agttcataac gcatgctgtc gtaagactga ccgtcaactt
     7081 tcatgtcgtt gctcagctgg tagtcatcac cagactggaa cagaccagaa acgctgccgt
     7141 aaggagtcac gtaaccagca tcacccagtt tgaagtcgta accggctttc aagccgaagc
     7201 cccaagcgtc ggagttggtg ctaccgtcaa cgtaagtacc gttgctcatg gttgcagaca
     7261 ggtcgttgtt gaagtgagag tagctcaagc taccatcaac aaagacgttg ttcgcgaagt
     7321 gagcagaaga gtagatgtag gcagtctggc tgtcttgatc tacctgacca gaacggtcat
     7381 tcatgtcacc tttagcgaag cctgcagccg caccgacgat ccacttagcg ttgttaccgt
     7441 caactttggt atcaacaccg accatgatgc cgttaacatc ctgatcatag ttgatggtgc
     7501 cgttgtcgcc gttgaagtta ccaccgaagt agcttaccca tgcgccgccg ttatcagcca
     7561 ggccatgacg agagttggtc agacgagtac caacggtgtc ttgttccagg ttccagatat
     7621 tggtgttcgc agacgggatg ctcagcgcca tgttagcgta gtcggtcagc tccatctgtt
     7681 gcagaacaac ggtgttaccg cgctgttcag cctgataggt gtatgcaccc aggtcagctt
     7741 tgttagcagc agagaaggtc gcgttggttt tggtatcatc gatatagatg agctgcttac
     7801 ctgcgtaatc agcaatcgca ccagcgccag tggagttgtc gatacgaact ttgtagttac
     7861 cggttgcagc tacaacgttg tctgcagagc tgttgtcaac ttcgtcgcca ttgttaatgt
     7921 cgttgttacc attaacggtc aagtgaccat cagagttcat cgcaataaca ccataaccgt
     7981 agttagcttt gctggtgtcg ttggtacgat cgttgacgag gtctgcattc aggatgtaat
     8041 cattactatg aatgtcaaac acacctgcgt aaacattacc gttgttatct ttaacgttgc
     8101 cactggtcag ttccagagta tcagttgcaa atacgctgga gatgtcattc atgttggtgt
     8161 ttacatccag cacaccaccg ttagtaatgg tattggcata cagcggtgca tatgcccaag
     8221 ctgaagcagt atcttcaccg aagttaacgg ttgagaaggt atcaatggtc aggtgatcgg
     8281 tgttaacttc accgttaact tccattgaag aaccaccgtt cagagtgatg gtgtcagaag
     8341 tcagagaaga atctgcactc acgttaacct gagaaccgct gttgatcgcc agggtatcga
     8401 tcagggaagt tttcgtggtt tcccaatcag aaccgttgtt cagaacaaca tcgaatacgc
     8461 cgctctggaa tgcgttagcg ttatcaacac gattccagcc atcttcagag taagaagcgt
     8521 acgggttcag gctgtttggt gcaacgtcgt agaaggcttc gtcagcaaca acaacgtcat
     8581 aatcaatatt cgctgaacca acccatttgg tgttgttcag agtcaggttc aggtgatcga
     8641 cgttctgatc gtcagttgcc caaccgccat gagtgtaagc agtggtagag ttgtcaatca
     8701 ggtgacccgt cgcgtcaaag ttagcgttcc aggtactgtc aaatacaacg tcaccaagca
     8761 actgagagtt gttgaacgta acgttgttga ccatacgata atcagaagtg tcatcatcga
     8821 tgaaggctaa cgcaacatca tcagcaggag atgcgccatt gccgtagtca cttggttcaa
     8881 cagaattgcc gaaatgacca cgatctgatt gttcgtcttc agtccatgaa ccagaagtta
     8941 cagtagagtc attaacaacg atgttgttgt cccactgatg accttcggta ttgtaggtcg
     9001 cattaccgag gtcgtgttga accaaagtaa tacctgcaac atgggagttg ttgctgatgt
     9061 tgatgttact ttcaacatcc agagtcacag cggtaccaag accagcggtt accaaacgat
     9121 cataatctgt cttagatgtt ttaccgtctg catccagata agtatcggtg aagtagaagg
     9181 cgtcaaaatc atcgtcaata gtagaattag cgatgttcag agtgaagatg tcgccatcaa
     9241 cccagtcgtg gccagtataa tcgctaccgt tggtccagac gtagtcgcca taaccaacct
     9301 ggtaagatgt aaccatacca tgaacaattg agttggagat gttcagtgta ttaccggtat
     9361 ctgtgccata atcgtggttc agataataag tagaaattac accatttaca gtgtcgccgc
     9421 tgaaagtggt atcaaagtaa ccatcagctg cgtggtcata tacataacca ccgtaagtca
     9481 gataatcgtg atcaatttta gtgctgtcac cccaattgta gatggtggct gcgtcatcgt
     9541 gatacccgaa ggtgttataa gtagctgcat tagcctgaga ggtgatagcc agagtgcaag
     9601 ccaatgctaa ttgtgatact acaagtttct ttttccagga gtgcatttgt catccctcct
     9661 cagggacgta ataagttgat ccatcaatta tcaatgcata gaaaacatat tatgtttcgc
     9721 gctaacgatt atgctgcgtt acagacgaat ggttcaatta ttctttcccc taagtccgaa
     9781 tgtggacata tataatttat ggagaaaaac acgcatcaaa ttgataaaag gacgatattt
     9841 caacatgttg cagataatgt tatttttgat acatacgcgt tataaatatc aaaaacacaa
     9901 accagataaa tatcttatca atatacaaca aagtttgaat tattcaattt atagcagcac
     9961 aaccaaacgc caaaaaaaat aataaccaag agctataaat aaagattaaa aatattttca
    10021 gtgaatatga aattattgct atttctatta caaagatgtc ctggtctctc tttcattctc
    10081 ttcttgtcaa catcacgaca actttcgtaa aacactcctg ccccgcttca ggtatactgt
    10141 gcaccttaat tccacaaaca tcaggcagac catgacagac ttaatccaac gccctcgtcg
    10201 cctacgcaaa tctcctgcgc tgcgcgctat gtttgaagag acaacactta gccttaacga
    10261 cctggtgttg ccgatctttg ttgaagaaga aattgacgac tacaaagccg ttgaagccat
    10321 gccaggcgtg atgcgcattc cagagaaaca tctggcacgc gaaattgaac gcatcgccaa
    10381 cgccggtatc cgttccgtaa tgacttttgg catctctcac cataccgatg aaaccggcag
    10441 cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg cgcatctgca agcagactgt
    10501 gccagaaatg atcgtcatgt cagacacttg tttctgtgaa tacacttctc acggtcactg
    10561 cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact ctggaaaatt taggcaagca
    10621 agccgtggtt gcagctgctg caggcgcaga cttcatcgcc ccttccgccg cgatggacgg
    10681 ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc tttaaagata cggcgattat
    10741 gtcgtattcg accaagttcg cctcttcctt ttatggcccg ttccgtgaag ctgccggaag
    10801 cgcattaaaa ggcgaccgca aaagctatca gatgaaccca atgaaccgtc gcgaggcgat
    10861 tcgtgaatca ctgctggatg aagcccaggg cgcagactgc ctgatggtta aacctgctgg
    10921 agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact gaattgccga ttggcgcgta
    10981 tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg ctggcgggtg ctatagatga
    11041 agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt gcgggtgcgg atctgatttt
    11101 cagctacttt gcgctggatt tggctgagaa gaagattctg cgttaatttt tttcctctca
    11161 gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc cctcacccta accctctccc
    11221 caagggggcg agggaaccgt ccgcactcgt atcaccccgc ccgataaaac ggtttatccc
    11281 caaggatcgt cgcccgatgc ataatccgcc gctgtggcag gtaatcggca ttggcatagt
    11341 gctgagtcac gcggttatcc caaatcgcaa tatcattcgg ttgccagcgc caacgcacct
    11401 gaaactccgg tttagtgata tgggcgaata aaaagcctaa caaggcttcg ctctctttct
    11461 cgctgacatc gacaattcgc gtagtaaagc cttcattcac aaacagcgcc tgtttaccgc
    11521 tcaccggatg cgttcgcacc accggatgta gcaacggagg atttttcgcg accgcctcgc
    11581 gccagcgttg atgttcctcc tcggttttgc ggtatttgta ttccgggaac gatttacgga
    11641 aatcatgctc cgcacgcagc ccgctcagca gctggcggaa gggaacagag agcgcctcat
    11701 aggccgcaat accgctggtc cagagcgtat caccgccggt cgaaggtaac tctttagctg
    11761 ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac atcggtatgc cagttgtcgt
    11821 tatccggcgg attatcgtta tgggtatcca gcacgatgat ctcgtcaacc ccttcggcat
    11881 gagggtaaac agggtgaata tgcagttcgc caaaacgctg ggccagcgcg cgttgctgct
    11941 gcggcgtaat agcttgatcg cgcagaaata ccacctgata gcgcagcacc gcatggtaaa
    12001 gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc ggcacccgaa atttgtgcgc
    12061 cgatatacgg ccccagcggg gtaatactca gacgttcact cattgtactt ctccatgcca
    12121 gggcgtcaga cggcgctgta acgcgcgcag acccagttct aaaagaaagg cgataatcgc
    12181 aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga aattcacccg ctgactgaac
    12241 cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc gctaccagcg tagaccagcc
    12301 cacacccaga ccaatgcgta atccggtgag gatttccggc agcgcgccgg gcaaaatgac
    12361 aaaccacagc acctgcgcac ggctggcacc cagcgactgg gcggcacgaa tgcgaacctg
    12421 ctgcgcgctt ttcacccccg ccagcaccga catcgccacc ggtgcaaaaa tcgctaaata
    12481 gatcaataag atcttcgagg tttcaccaat accaaaccag atcaccatca gcggcaaata
    12541 agccagcggc ggcaccggac gataaagttc gattaccgga tccagaatgc cgcgcaccgt
    12601 cgggcttagc cccatggcga tcccgaccgg aataccgatc accaccgccg cgagtagcgc
    12661 cagcacaatg cgcgtcaggc tggctgccag atgctgccac agcgtggcgt ccataaatcc
    12721 ctgcggtccg gcaatggtga gcagttttgc cagtacttgt tgcggcggcg gcaaaaacag
    12781 cgggctaatc agttgcagcg ccgccaccgt ccaccataca gtgagtaaaa ccgctaacgt
    12841 gccaatgctt aaggtcacct gacgcgagag cggccagcgc catttcagcc gccgcgaatg
    12901 cagtttttca ttaatgagca cactcatgag aacgcctccc gttgctcaaa tacgcggctt
    12961 aaaacatatt cgcgcatggc gatgaattgt ggatcggact tgatgctgcg gctcgactct
    13021 cccgcaacaa agcggcgagc aaagttgagc ggcagccgct ccagcacacg gccagggccg
    13081 gatgaaagca gaaccagttc agtcgccata aacaccgctt cttctatatc gtgggtaatc
    13141 aacagcacct gcttgcccgt ctcctgccag agtttcagca gcagagtttg catctggtcg
    13201 cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta ataacagctg gggattcgcc
    13261 gccagcgcgc gggcaatccc cacccgctga cgttgaccac cggaaagctg ccagatatag
    13321 cgtttttctg cgccttccag ccccactttt ttcagcatct ggtgcgcgat ttccagtcgc
    13381 tgcattttct ctatacctgc caattgcagg ccgaacgcca cgttgtcctg tacattgcgc
    13441 cacggtagta gcccttcatt ctgaaaaacc accccgcgct ctgctcccgg tccctcaata
    13501 cgcttacccg ccagttgaat gctgccatgc tgataaggca caaaaccggc aatcagattc
    13561 agcagggtgg ttttaccgca gccggacggc cccagcacca ccagtagctc gccgctttcc
    13621 agcgtcaggt tgatatcttc cagtgccggt ttgccgccat aatcggcata gagatgagag
    13681 atttgcagca tctgcgcctc cttttattgc acgaagcgcg aggtaacgta ctggctgtaa
    13741 tcattcgcta cagccgggac cttgccctgc tctttcaaaa actgcgcggt gtcgatgatt
    13801 gctttgttca ccggtccggt cagttctgcc gtttgttgct gcggcgtcag ataggtattc
    13861 cccttcacca gccccggaac gtcaccttcc ggcacgccgc ttaaacgcgc cagtttgctg
    13921 atgttttccg gctgtttcag ccacgcgtct gggttagcaa tgtacggttg ctgagcatcg
    13981 atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt tctcggcaaa atctttgcgc
    14041 accacccaga cgtccagcgt tggcgcaccc cactgcccga cctgttcaga atcggtcaac
    14101 accttgccgt ctttttccag ggcgttaacc gccggtgccc agacataagc accatcaata
    14161 tcaccccgct gccatgcggc gataatcgcg ggcggctgca ggttcacaat ctccacttgc
    14221 cctggtttaa tcccccagtg tttcagcgcc gccagcaggc tgtagtgggt ggtggagata
    14281 aacggtacgg caatgcgttt gccaatcaaa tcttcgggtt tgctgatagt tttctttacc
    14341 accagcgctt cggagttacc cagttttgac gccagcaaga agacttcaat cggcacctgt
    14401 tggctggctg caaccgctaa tgggctggaa ccgaggttgc cgatttgcac gtcgccagaa
    14461 gccagcgccc gcacgatgct ggctccgctg tcaaacttac gccagtccac gctcgccccg
    14521 ctctctttag caaaggtgtt gtcggcctga gccactttcg ccggttcggc tgaggtttga
    14581 tacgccacgg tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag tgcggcaaga
    14641 agtgtgttac gcgatgaaat tgccatgatt gtctgctccc ctgtcttgtt atgggagcag
    14701 tattcaggaa taaaaacatt cattaaaaga attagtcgtt atcgcacaga tgattttatt
    14761 cttagcaaaa aaacggcaaa ggaaagat
//
LOCUS       DAZOXR010000098        14762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZOXR010000098 DAZOXR010000000
VERSION     DAZOXR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.100"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            198..848
                     /locus_tag="ACHUNN_002611"
     CDS             198..848
                     /locus_tag="ACHUNN_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576954.1"
                     /translation="MANIIISKKSIIEAASIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKAGKCVYSVADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGMGSAKAGTQQYRFIKNDFYNYLADAFKA"
     gene            898..1101
                     /locus_tag="ACHUNN_002612"
     CDS             898..1101
                     /locus_tag="ACHUNN_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576955.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELINDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLHNSI"
     gene            1122..1349
                     /locus_tag="ACHUNN_002613"
     CDS             1122..1349
                     /locus_tag="ACHUNN_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576956.1"
                     /translation="MVIFISGVNINNHTLVYDIAGLAGYALSSEVVDETTFKININDV
                     EHRARAGINEADVTLMLQEFLNAGFNIRLEK"
     gene            complement(1968..2450)
                     /locus_tag="ACHUNN_002614"
     CDS             complement(1968..2450)
                     /locus_tag="ACHUNN_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576957.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(2801..3175)
                     /locus_tag="ACHUNN_002615"
     CDS             complement(2801..3175)
                     /locus_tag="ACHUNN_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576958.1"
                     /translation="MGLDIYIETQPKNDLNNDASRKKVAYFRKFNALFGWMERNVGEV
                     KNCELLELTMNDICALKAHLMHMNESNCEEYLPTCEGFFFGSQEYDEGYWHDVGELKK
                     LVEELIKNHDFHNNRLTFCAWW"
     gene            complement(3294..3689)
                     /locus_tag="ACHUNN_002616"
     CDS             complement(3294..3689)
                     /locus_tag="ACHUNN_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576959.1"
                     /translation="MSVKRENLTVDVYYASESAEGKNVAKITVVTYNTETGTEVQAST
                     IVRKGDTSGGEYEAQYQSIFDATDPLLLKIESYFRKVNKEVFETMMNMVNTVFASSLN
                     TNTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            complement(3815..4126)
                     /locus_tag="ACHUNN_002617"
     CDS             complement(3815..4126)
                     /locus_tag="ACHUNN_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576960.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            complement(4281..4610)
                     /locus_tag="ACHUNN_002618"
     CDS             complement(4281..4610)
                     /locus_tag="ACHUNN_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576961.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     DFLAIFA"
     gene            complement(4749..4964)
                     /locus_tag="ACHUNN_002619"
     CDS             complement(4749..4964)
                     /locus_tag="ACHUNN_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576962.1"
                     /translation="MKMPVTVEVWGVDSLAECLDAVGPELYRKLWSFVPAEGESPKGK
                     DIWHLLSEDEKRELVGAVHSEFPDDQD"
     gene            complement(6537..6722)
                     /locus_tag="ACHUNN_002620"
     CDS             complement(6537..6722)
                     /locus_tag="ACHUNN_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576963.1"
                     /translation="MLKEDSYLLKALEMSGVEPTVKNAHDIYLQFSTELKQLINKYGV
                     NDSSVKAMIDDELDHLI"
     gene            complement(6759..7058)
                     /locus_tag="ACHUNN_002621"
     CDS             complement(6759..7058)
                     /locus_tag="ACHUNN_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576964.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIEYGCI
                     VHAGLQDDDWKDELRRYGISEGAIANIQKVLDVGFNSVHFDCGAPVVEGLECWQW"
     gene            complement(7220..9253)
                     /gene="metG"
                     /locus_tag="ACHUNN_002622"
     CDS             complement(7220..9253)
                     /gene="metG"
                     /locus_tag="ACHUNN_002622"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0576965.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKCMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLTSELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADEP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            9411..10511
                     /gene="apbC"
                     /locus_tag="ACHUNN_002623"
     CDS             9411..10511
                     /gene="apbC"
                     /locus_tag="ACHUNN_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0576966.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(10549..10938)
                     /locus_tag="ACHUNN_002624"
     CDS             complement(10549..10938)
                     /locus_tag="ACHUNN_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFR0576967.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKSPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(11111..11635)
                     /locus_tag="ACHUNN_002625"
     CDS             complement(11111..11635)
                     /locus_tag="ACHUNN_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR0576968.1"
                     /translation="MTKFGKLEAHLLTRNYRYDVHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRAHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGESLVKSFGKGFVAPDLDEL
                     QPNEVSHLIFSHSQ"
     gene            complement(11649..12503)
                     /locus_tag="ACHUNN_002626"
     CDS             complement(11649..12503)
                     /locus_tag="ACHUNN_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576969.1"
                     /translation="MMHKSVAGEFQKEVDNTTVLLDDILSILALLETGDWSEHCTKTE
                     LGGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSNRVE
                     KVDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWGECTRAAY
                     VRAKDNGELTRVVTNHPNNELKDHQIRELVNELRDIAVTYHGTQQLREKIARAVNNSL
                     KGGQSQPKRIRITHIGENGTEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLRAMEK
                     YGDKWEVL"
     gene            complement(12500..12721)
                     /locus_tag="ACHUNN_002627"
     CDS             complement(12500..12721)
                     /locus_tag="ACHUNN_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576970.1"
                     /translation="MNKAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRCYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            complement(12714..13289)
                     /locus_tag="ACHUNN_002628"
     CDS             complement(12714..13289)
                     /locus_tag="ACHUNN_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0576971.1"
                     /translation="MTTITDKKQYPGEQYLNELITNIEFAARAPVEVVRAMAAELQKR
                     READSAEPASNHEELPLDYLQGQKDGLEWAAQLAEANHPQTGDWLYDDPLDLAKAIRK
                     GPDIPEFAGPHPVTPDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGITFR
                     RGDVTIKPGNGIEQATHWMPLPEPPQESKSE"
     gene            complement(13291..13677)
                     /locus_tag="ACHUNN_002629"
     CDS             complement(13291..13677)
                     /locus_tag="ACHUNN_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576972.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPVPVVPEEKPMPNPLSMYA
                     VDAVAAIAEVRGWNACRAAMLQGKGE"
     gene            complement(13688..13951)
                     /locus_tag="ACHUNN_002630"
     CDS             complement(13688..13951)
                     /locus_tag="ACHUNN_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576973.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDERILFPSSVESTAG"
     gene            complement(13953..14474)
                     /locus_tag="ACHUNN_002631"
     CDS             complement(13953..14474)
                     /locus_tag="ACHUNN_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576974.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGKVMIFPAQPLTTLTPEQVADITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIRIKGE"
     gene            complement(14471..>14762)
                     /locus_tag="ACHUNN_002632"
     CDS             complement(14471..>14762)
                     /locus_tag="ACHUNN_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFR0576975.1"
                     /translation="KAFHILERLEALATEEEISPEKLVEFSRVILRRKNDMERLTSGA
                     PSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGEKEGSA"
BASE COUNT         3768 a         3505 c         3301 g         4188 t
ORIGIN      
        1 agccggaatc gattatttat ttagggaaca ccctcgacga tctaacattt atttctagga
       61 ctttcatccc tgcaaaaaaa cttaaaaaaa cacttgcaat cttttttcgc gtatcgataa
      121 ttgaacgcat caggaacgag tgcaaaacac tttctttctt gagttaaatt ttaatattta
      181 tactaaggaa taaaatcatg gctaacatta tcatttctaa aaagtccatc attgaagctg
      241 catctattgt atccgacgaa ctgcgcgaaa aagcagatct ggcaactcaa acatataacg
      301 aacattataa aaatggtacg cacactaaag cagacaaagc aaatatgcaa gctgcgacca
      361 ctaaacttgc ttacttcatc aacaacgtcg taaacgctgt agaagacgaa aaattatgct
      421 ctgttttcta ctatgcgatt aaagcaagca aacaagcgcc agaagtattt tttcgtgacg
      481 ctatgactaa tagttattca ctggaaaaac tggtttatct ggttaaatca attaaagctg
      541 gaaaatgtgt ttattccgtc gctgatatgt ctggatctcg tgtattcgct ttaatcgata
      601 tgattaacga cgagatcgac acgttcacga atggcgctgt tttcgattta atgaatgaag
      661 ccaaaaaagc gtgtgaaatt aaattggacg ctggctatac tcaagctaac cagctaatca
      721 atctttgcga acgtctcgga cttgttgaga aagtcaaagg aatgggaagc gccaaagctg
      781 gtacacagca atatcgcttt atcaagaatg atttctacaa ttatctagct gacgctttca
      841 aagcgtaatt aatggaatca agcgcccact atgggcgctt taaaggagct ttaaaaaatg
      901 gtcagctatg acaagatccg cgcggaatat cgcgcaaagt atcgcgctta taaactagaa
      961 ttaatcaatg atttaatcgc gcaacgcgac caattaaatt ttacgttttc tgatttgctt
     1021 aacagcaaac gagattgcaa gcgtaaacgt gaatacttac gtttaagcgc attaatcgga
     1081 aaacttcata attctattta atttttttaa ggagcttaat catggttatt ttcatctctg
     1141 gcgtaaatat caataatcat actcttgttt atgatattgc tggtttagct ggatatgctc
     1201 taagttctga agtggttgat gaaacaacgt ttaaaataaa catcaatgac gtagaacata
     1261 gagcgcgcgc aggaatcaat gaagcagatg ttactttaat gcttcaagaa tttttaaacg
     1321 ctggttttaa tattcgctta gaaaaataaa aacaatagcg cccatagtgg gcgctttttt
     1381 cgtttccaat actcccacca taacgcgcca atgatggcgc gttttttatt gtctttgact
     1441 cactccaaca acataaaaat aagcgccaaa ataacgccat agacgcgctt ttatatcctt
     1501 gccagtacat acccattact taacacatta acgcgcttaa aacgcgttat attgcgctat
     1561 agcgcatagt taatcattgg cttttagtct tgctatgtga tccgcgttta tttgtcggcg
     1621 cggatcggca ttttgttttg ttccgtatcc gctcgcgtat tatgtgcgcg tgatttttca
     1681 cataatcaca ccacttaatc acgtatgcga ttacgctacg aaagaaatct ggatgtctca
     1741 ggcgacgaaa gtcatcataa ttttccccgc tcacctgtcc gacaaccgct ggttggattc
     1801 caccagcttc ccgatgtttt ttctacataa aggcgaatgc agccgtttcc cgaaaaaatc
     1861 ctggccgttc cccgtcggtt catgaatacg ttcctcgccg tttctgaaaa tttccctgcg
     1921 gcatctggtg gctatagaga aagggccgtt actggccctc ttctcagtta cacgccatca
     1981 aggatgtgga tgcggttgct tgcgtataca ttcagcataa agttagcgca aaacagtttc
     2041 catgtatcaa cgccagcggt atacgtaatg tttttgcatt taattgcatt gttggcgatc
     2101 cgcatcccct gcgatactgc ttcatcgtcg gaaaatttga acgacgattg agttttaatc
     2161 cagacagaaa tctgcgtagc gaactcaatc agcttggact ggcagaatcg cccggagcgc
     2221 accggaaaga cgaacgttcc gaatccagaa tttaccacat acgctttctc aaatacccgc
     2281 gagtaacgac gattgccaat gatgtcgcgt gcaataatgc atttttcttg tgctgacagt
     2341 tctaccgtct cattgtcgcg ccatgcacca agtactcgtt tttcaatgtc agagaacgtt
     2401 acagcgatat tgccatgtgc gggtgcgttt acagtagcga taaaattcat gattaattcc
     2461 ttatcgtaaa taacaaattg ttttcttgtt ggcgttaatt atcgttgtac gaataaggcg
     2521 tcaaagtgga aagttgcggt agccggacgg gaacaggtgg gtttgtcggt tgcctggagg
     2581 taagaggttg gtgtttttag cctgcgggaa acaggatggt catttaaggc caccagcagt
     2641 ggtggcctta ataattatcg accgattacg ctaaggatct tttcctcaac tgatttgttg
     2701 ttgcgactaa actgccttgc gtatctgatg acagaagagg cgttttgctt tctgactatc
     2761 tcggctcttt ccttcaggcg tttcttgaaa tcgcccatat ttaccaccag gcgcagaagg
     2821 tcagtctgtt attgtgaaag tcgtggttct taatcagctc ttccacaagt tttttcaact
     2881 cccccacgtc atgccagtac ccttcatcgt actcctgact gccgaagaaa aaaccttcac
     2941 aggtaggtaa gtactcttcg cagttacttt cgttcatgtg catcaaatga gctttcagag
     3001 cacaaatgtc attcatcgtt aattctaaaa gctcacaatt tttgacttca cctacgttgc
     3061 gttccatcca cccaaagaga gcattgaact tacggaagta agcaaccttc tttctggatg
     3121 catcgttatt cagatcgttt tttggttgcg tctcaatgta gatatcaagt cccatgatgg
     3181 tttccttatc aagttgcttt cagtgaaagc attttcacaa atcagataag gcagaaaaca
     3241 aattgttatc gggcataaga aaatggcgcg gtttacgcgc cattcaaaga ggattaagcg
     3301 aatacgcttt cagggattaa ggtgtcagca ggaatgccgg aggtaatgcg cagaccatac
     3361 tgaccaatcc aggtagtgtt agtgttcaga ctggaagcga atacggtgtt aaccatattc
     3421 atcatggttt caaacacctc tttattcacc ttacggaagt aactttcaat tttcaacaac
     3481 aacgggtccg ttgcatcaaa aatggactga tattgcgcct catattcccc gcctgatgta
     3541 tctcctttac gcacaatagt acttgcctga acttcagttc cagtttcggt gttgtacgta
     3601 acgacggtga ttttagctac attcttacct tcggcgcttt ctgaagcata gtagacatca
     3661 actgtcaggt tttctcgttt aacgctcata tcactctcca tagtgtcttg gttatttttc
     3721 tattttatag acataggtat gtacttacaa tacaaaaaac cccgtaagga tggcggggct
     3781 gtcgtactgt cgactaatta ccacttgcac attatcaggc tattgccatc actgatgctc
     3841 tctgaatctc ttgctgggcc acgcggttaa cttccatcaa agccagttcg agctgatgtt
     3901 ccggccacat tctttgaaat gccatccagc cttttccacg ctggcgacga acattcatta
     3961 cataacgagt aaaggtgttt ttatcgaaag acaccgctgt ttcacggaac agacgaatag
     4021 aggttccatt ggcaacgatg tccagcaaag ttaattgagc aaccagtgca ggcttctctt
     4081 ttctcttctg gtctttgtcc agaccaagaa ccaatgattt tttcatcaca actccgtaaa
     4141 caacttgttt tctcaatggc gtaaataata ccagtaagaa aacggctaca aagcatattg
     4201 atcaggcgca tgagcgatat gcaggttctt ccctgcggct acagatgaag ttaagccagc
     4261 aatgctggct ttggttgggc ttacgcgaat atcgcaagga agtcctgaac gtcccaatgc
     4321 tggacgaggt gaagatagac cttagaaatc acgtcatttt cgctatcgca tgtgaagtag
     4381 tacttctggc tgcggttgta tgtgtccaca caaaattcgc tcatatccat aaagtcagca
     4441 tcttcaccca tgtcgcaaat catcaaagca ggtgcgtcat cacgagtcat cagctccagc
     4501 acccatcttg aattaatcag agtggaagac caaccttcca gctcataaaa ctggtcaaac
     4561 tctttttgag tcaatttctt aatttggttg atgtcgatat ttgcaaacat aaagcaatcc
     4621 ttaaacatgt tgttttctga attacgtaaa caataccagt aagaaaacgg ccacaaagta
     4681 tatcgttacg gtgctgcgct gccccgaggt taatagcgac aaaagccacc gtgctggtgg
     4741 cctcactctt aatcttggtc gtccgggaac tcgctgtgta ccgcgcccac cagctcccgc
     4801 ttttcatctt cactcagcag gtgccagata tccttccctt tgggcgattc cccttctgcc
     4861 gggacgaacg accacaactt gcggtacaac tccggcccca ctgcatccag acactcagcc
     4921 agggaatcca cgccccacac ctcaaccgtc acaggcatct taatcatctc ttcatcctca
     4981 cttcgtttct ataacgtcta aatatgctgt ttacttgttg gtgttattat cgcaataaga
     5041 taaaggcgaa aaaggatttt atgtcgggaa agccaggctg ggcagtgaat atccgggaac
     5101 gacacgagag ccagtattta cgatattctg ctctacgcgc gccgcggggc tggtgggtag
     5161 aataatcctc tccccgaaaa cctactgaaa tcgattgtga cccgctggct gtctggcaca
     5221 attacgctgt atctggcatt cagagggaat ccagcggtaa gcaagtcagc ctgcacgtga
     5281 gcgcctctct tcttcccgca cattacaggt gggtagctgc aactctctct ctcgctatac
     5341 cggtacgccg ccgttccaga accggcatcg agccttttcc cgaaatccac acaggcaact
     5401 cgaccgtttc cctgaaaatc cccagccgtt cccccgaagg caacccagcc gtttctgtag
     5461 ctggatgcaa ccttttccct gtatggaccg aaggctgacc gtagggggtg ttacggggga
     5521 taaaatcagg agttgaataa atacgaaaaa ggaggcgacc cccctccttc cctctccact
     5581 tatacatacc tggcttcttc gctatacatg ttgtgtttta tctgtctcaa cctctctata
     5641 ggcgttaatg ctcactcctt ccagaatgtt ctatacggcg actatttctg ttggtgaatt
     5701 gggtgtgttg ttttctctgt cggtgtatgt ggtttcgtta gttttgcggt ggttcttcta
     5761 tgggggtgtt ctcgtcgttt tggtgattgt tcttctccgt gtatggagta atggcaggtg
     5821 ggcgattgct ttcgttattg tgtttgagtt gtttgtctgg agtgtgtgaa ggcttttgta
     5881 gttattcgtg tattgggtaa tggcgtttcc cgtgattgtt atgtttcgtg tcttttggga
     5941 ttgagagaaa ccttgcggtt acaggcttcg ttagggtttc agcggttcag gtgtttcttc
     6001 gctgtttggg taatggcgtg tcgtgggtat cgcgcgtatg aatgttttcg gattgcctgt
     6061 gaaagttagg ttggttgtcc ggtagcctgg ggagaagagg tgggtctttt cggtagcctg
     6121 ccataaagag gttggttgtt ttgggtaatg gcatatgtgt tatttggttg ttttgttatg
     6181 cctgaaaata agttgtttat atggttgtgt aacgcaacgg gagcgatttt gagcgtgtgt
     6241 ttttgttggt agtgtgttga gtcgtctgtg gtgaaatttg gacgctgtgg cgctatctgc
     6301 tgcgtagaat tacgggtata agaagtgatg tgtgataacg tcaatttttt agaccaaatc
     6361 gggagaaagc gttgatgttt cgttgatctg ttatttagtt gttttcttat tggtataaac
     6421 aaccataacc tattgatatt taatgatgtg attgctggta tgggagggta gtagtggcga
     6481 tcaacgggtt cttatagaaa agaatcaaat tgtgaccgcc attatcaacg gtttgattag
     6541 atgagatggt ctaattcatc atctatcatg gctttgacgc tggagtcgtt cactccatat
     6601 ttatttatga gctgtttcag ctctgtcgaa aactgaaggt agatatcgtg agcgtttttc
     6661 actgtaggtt caacgccact catttcaagc gcctttagta gatatgagtc ttctttaagc
     6721 ataaattctc cgttatctag tatgaagcga ttgccggatt accattgcca gcactcaagc
     6781 ccttcaacga ccggtgcgcc acaatcaaaa tgtacagagt taaagcctac atcaagtact
     6841 ttctgaatgt tcgcaatcgc accctcggat ataccatatc gacgcaactc atctttccag
     6901 tcatcgtcct gtaacccggc gtggacaatg caaccgtact caatttgtga aacccaatcc
     6961 agctcattgc ttgcgatcat gaatcggcga gcatcttcct ctgatgtatg agatgaactg
     7021 atcaatgcca cctggtaggc ttgggtaatg ttcaacattt caactactcc aaaacaagtt
     7081 gttttcttat gtggcttatt atctcaatca acaaaaggta gaaaacaaat tgttttaggg
     7141 cttgtgtaaa tcaggaggga ttgtgtctat atgaaaatgc ccggcgtgtt gccgggcatt
     7201 gcgaagagag gttgtttaat tacttaacct ggtggccagg tttagcacca gcatccgggc
     7261 ttaacaggaa gatatctttc ccgccaggac cggcagccat caccatgcct tcagagatac
     7321 cgaagcgcat tttacgtggt gccaggttag ccaccataat ggtgtgacga ccaatcagtg
     7381 cctgtggatc tggataagcg gaacggatgc ctgagaagac attacgcttt tcaccaccga
     7441 gatccagcgt caggcgaagc agtttgtcag aaccttcgac aaactccgcg ttttcaatca
     7501 gcgccacacg caggtcaact ttggcgaagt cgtcaaaggt gatggtttcc tgaatcggtt
     7561 cgtcagccag tgggccagtt accggtgcag ctgtggcttt cacttcttct ttggatgctt
     7621 ccaccagtgc ttccacctgt ttcatatcga tacggttata cagtgccttg aacggattca
     7681 ctttatggcc cagaagcggt tgctggatac catcccaggt cagttctgta ttcaggaatg
     7741 cttctgcacg ttcagtcagt ttcggcagta ccggtttcag gtacgtcatc agcacgcgga
     7801 acaggttaat gcccatagag caaatcgcct gcagatctgc atcgcgacct tcctgcttcg
     7861 ccaccaccca cggagcctgt tcatcgacat agcgattagc caggtcagcc aatgccatga
     7921 tttcacggat agctttacca aattcgcggc tttcccatgc ttcgccaatc acttcagcgg
     7981 catcagtaaa ggttttgtac agttgcgggt cagccagttc gcttgtcagc acgccgtcaa
     8041 aacgcttgtt gataaagcct gcattacggg aggccaggtt aaccactttg ttcacgatat
     8101 cggcattcac gcgctgaacg aaatcttcca ggttgagatc gatatcatca atgcgcgaag
     8161 agagtttcgc agtgtagtag taacgcaggc tgtcagcgtc aaaatgattc agccaggtgc
     8221 tggctttaat aaaggtgccg cgagacttgg acatcttcgc gccgttcacc gtcacatagc
     8281 cgtgaacaaa caggttggtc ggcttgcgga agttgctgcc ttccagcatg gcaggccaga
     8341 acagactgtg gaagtaaacg atatctttgc cgatgaagtg gtacagctcg gcggtggagt
     8401 ctttcttcca gtattcgtcg aagcttacgc tgtcgccgcg cttgtcgcac agattcttga
     8461 aagaacccat gtagccaatc ggcgcgtcca gccagacgta gaaatatttg cccggcgcgt
     8521 tcggaatttc aaaaccgaag taaggggcgt cgcgggagat atcccactgt tgcaggccag
     8581 attcaaacca ctcctgcatt ttatttgcca cctgctcctg caacgcaccg ctgcgggtcc
     8641 atgcctgcaa catttcgctg aaagagggca gatcaaagaa gaagtgttca gaatcacgca
     8701 ttaccggcgt agcgccagaa accaccgatt tcggctcgat cagttcagtc gggctgtagg
     8761 tcgcgccgca gacttcgcag ttatcgccgt attgatccgg ggatttacat ttcgggcagg
     8821 tgccttttac aaaacggtcc ggcaggaaca tgcatttttc cggatcgtac agctgtgaga
     8881 tggtgcggtt tttaataaaa ccgttttctt tcaggcgaga gtagataagc tcggacaact
     8941 gacggttctc ttcgctgtgc gtcgagtgat agttgtcata gctgatgtta aagcctgcga
     9001 aatcagtctg atgttcctga ctcatttcgc caatcatctg ttccggtgtg ataccaagct
     9061 gctgtgcttt cagcatgatt ggcgtaccgt gggcatcgtc ggcacagatg aaattaacct
     9121 cgtggccgcg cattcgctgg taacgaaccc agacatcagc ctggatgtgc tccagcatat
     9181 ggccgaggtg gattgagccg ttagcgtacg gcagcgcgca cgtcaccaga attttcttcg
     9241 cgacttgggt catagtaggc attacttctt tgtagtgaaa aggggcttga tagtaacaaa
     9301 atggccttat gtctgccatg tgataacagc atttctcata aatgattaaa tgtcgtagct
     9361 ggagtacact acaaggcgac aattgcgcaa attaaaataa aggagacggg atgagcgagt
     9421 ccaaatcgcc ggatgccctg agagcaatgg tagccggtac gctggctaat tttcagcacc
     9481 caaccctgaa acacaacttg actacgctta aagcgttaca ccatgttgcg ttgatggatg
     9541 acacactgca tgtcgaacta atcatgcctt tcgtgtggaa taagcctttt gaagacctga
     9601 aagagcaatg tagtggtgat ctgctccgta tcactggcgc aaaggctatt gactggaagc
     9661 tgtcgtacaa cattgccacg cttaagcgcg tcaaaaacca accaggcatt aatggcgtta
     9721 agaacattat cgccgtcagc tcaggcaagg gcggcgtggg taaatcctcc acagcagtaa
     9781 acttggcact ggctttggct gctgaaggtg cgaaagttgg tattttggat gccgatatct
     9841 atggtccatc aattccaacc atgctgggcg cggaaaacca acgtccaacc tcaccggacg
     9901 gtactcacat ggcacctatc atgtctcatg gcctggcaac caactctatt ggttatctgg
     9961 tcaccgatga caatgcaatg gtgtggcgtg gaccgatggc gagcaaggcg ctgatgcaga
    10021 tgttgcagga aacactgtgg cctgatctgg actatctcgt tctcgatatg ccaccgggca
    10081 ctggtgatat tcaattgacg ctggcgcaga acattcctgt aactggtgcg gttgtggtaa
    10141 ctacgccgca agacatcgcg ctgatcgatg cgaagaaagg cattgtgatg ttcgaaaaag
    10201 tcgaagtgcc ggtactgggt atcgttgaaa acatgagtgt gcatatttgc agtaactgcg
    10261 gtcatcacga gccaattttt ggcaccggtg gagcacagaa actggccgag aaatacaaca
    10321 cgcagttgtt aggccaaatg ccacttcata tctctcttcg tgaagatctg gataacggaa
    10381 caccaaccgt tattagtcgt ccagatagcg aatttacggc tatttatcgt gacctcgcag
    10441 atcgcgttgc tgcccaaatg tattggcaag gtgaagtaat acctggcgaa atcgcattcc
    10501 gcgcagtgtg ataaaaaggc ggtgcattgc accgcctaat cagttctcct acatcacagg
    10561 acaatcatca aactcgccac ttcttgcgtc gttgataatg tgagtgatca caccaaacac
    10621 ggcattgctg cccgtgcatc catcgtcatc ctttggcaat gcttccttct taccggttct
    10681 taaatcctcc agatgctgac gtggagactt ccggtatctt tttacgcgat attccccatc
    10741 cagagcgcat acaagcagag aaccatcaac cggggtaagt gaggaatcaa ccaccagcaa
    10801 tgcaccctgt aatattccct cacgatgatg actatcagct gctctcatga agtaggtcgc
    10861 tgaaggatgt ctaattatct gctgatcaag agaaatacgg ctctcagcat aatccgccgc
    10921 aggagaaggg aagcccatag cgttttgccc tcaatactgt tcatttacac agtatacatg
    10981 aaagaagctc actcagtgca actgatcggt acatagcacc agacaaagga acgttatttt
    11041 ctcgaacaag aaaaggtaga aaacaaattg tttaaggcca caactagtgg ccttaatgga
    11101 gaagggatgc ttattggcta tggctaaaaa tcaaatgaga cacttcattt ggttgtagct
    11161 cgtcaagatc cggggccaca aaaccttttc cgaaagactt cacaagtgat tcaccagccg
    11221 catcattgtc acctattacg cgaaagtcat acggcagcat tgccagttga cgacgtaatc
    11281 ctgctgagaa agtcgaacct aacagcgccc acgaatttcc gcccgcgttg tgcactgctg
    11341 cacttttaaa aatcgactca gttatccata tcggtccatc ttttaaagga agataggttc
    11401 cccatagtag ctgtctgccg tgcgctcgtg tgaaataccg ggccatcttc ggattgctat
    11461 gctgcttagg agcgcctggt ctgtatgtct gatacccggc caacatccca tcaaacctgt
    11521 atagagggaa tgtaacaact ccttcgcttt catcgatcca ctgtctatat ggatgcacat
    11581 catagcggta gttacgagtt agcaaatgag cttccagctt tccaaattta gtcataactt
    11641 cgcatccctc ataacacttc ccatttatca ccatatttct ccatagctct tagccaataa
    11701 acatggccac taggcatagt gatgtgaggc tcgccaaact cttctccatg tacatcaaag
    11761 atgtccccaa cagaaaattg ggggatttct gttccgtttt cacctatatg tgtaattcgt
    11821 atacgtttcg gttgggattg tccacctttg agcgagttgt tcactgctct ggcaattttc
    11881 tcccgcaatt gctgcgttcc gtgatacgta accgcaatat cccgcaactc attcactagt
    11941 tctcgaattt ggtgatcttt aagctcatta tttgggtgat tggtaacaac tctggttaat
    12001 tccccattgt cttttgctct tacgtatgca gctcttgtgc attcgcccca tgtattaccg
    12061 cctacattaa cttcgtagat taggctggac ttatctgtca attcggaaca aatacgggcc
    12121 gtggtctggc aactagagca ttcgcagtta tcgcggaaac cgtggtctac tggcttcggt
    12181 ttaacatcat caactttttc taccctattg gaatttaaat acttcgaagc cttgctatgc
    12241 caatcgctgg ttttaggatc ttgtccatcc atcaaacgcc aggcttcagc tattaactca
    12301 taactagtga ctgtagcttc ctgcgcatca ccaataagtc gtgtaatctc tctttcaagc
    12361 cgaccgccta actccgtttt agtgcaatgt tctgaccaat cgccagtctc aagaagcgcg
    12421 agaatgctta aaatatcgtc caatagaacg gtagtattat cgacttcctt ctgaaactca
    12481 ccggctactg atttatgcat cattcgtcat cctcatccga gtcatcctca tcacaggatg
    12541 agagtagtgg gttagtcatt cgccctactt gagtggcgta gcaacgccga cagagattgc
    12601 gcagcacact gtatatttcg aacatctcgg ttcgttcatc gccaacatca agctcagatg
    12661 ctatagcgtg gcattcagtc gcgagagccg atattttctg aaataactct gctttattca
    12721 ctcttcgact cctgcggagg ttctggtagc ggcatccagt gagttgcttg ctcaatacca
    12781 ttacccggct taatcgttac atctccgcgc cgaaaggtga ttccggtata gcgtgcggag
    12841 catattagcg gttcaaccag agagctatcg aaattcaccg aaataagcac gttctggccc
    12901 ttttcaggca ttcgctcact acagcttatc caaccatccg gagttaccgg atggggtcca
    12961 gcgaactcgg gaatgtcagg accttttctg atagctttag ccagatccag cgggtcatcg
    13021 taaagccagt cgccagtttg tgggtgattt gcttctgcaa gctgcgcagc ccattcaaga
    13081 ccatcttttt gaccttggag ataatcaagc ggcaactctt catgattact tgcaggttct
    13141 gcactatcag cttcgcgccg cttctgtagc tctgctgcca tcgctctcac gacttcaact
    13201 ggtgcccttg cagcaaactc tatgttggtg atcagctcat taagatattg ctcgcctgga
    13261 tactgtttct tatcggttat agtggtcata tcactctcct tttccctgaa gcatagcagc
    13321 gcggcaggca ttccagcctc tcacctctgc aatagcggca acagcatcaa ccgcgtacat
    13381 gctaagagga ttaggcattg gtttttcttc cggtactact ggaacgggtg gagcggcgta
    13441 gacctcaata attccattat caataggcca ttctccatcc ttgaggtagt cacttgtgcc
    13501 gtcaacttgc tgttctgcaa tgtggaatgc acctattggt tttgcttcca gcgatgccag
    13561 agcaattcgt gccagttctt ccgcttcttc tgctggcagt acaacgttgc taccaggtcc
    13621 gtatgtttcg cgccactgtt tgattgtcag tagtcgctct ttggtaatag tggtcatgtg
    13681 ttactcctta acccgcagtg ctttcaactg atgaggggaa caaaatcctt tcatcaaatc
    13741 cggcattcat atcatggaca gcaacacacc aatccatcga cgaacgatta tcaagagcct
    13801 ccatgatttc atccatgcgg cgcaggtcat acaggtaaat gcttttatcg ccaatggtgt
    13861 aaaaaccaat ttttttcggt gatgggcagc gatcaagaac gtcctgtaat tcgttcaacc
    13921 atgcccgttc tttttttgtt aaagttgcca tatcactctc ctttgatgcg aatgcctgct
    13981 gcaatgctgt ttatgatgct gtcagtgcat ggagtagaaa gctgggcatc tccagcaatt
    14041 ttcatgacct caacatccgc atatcgaata ccgaggtgta tcagaccggc tatacctgac
    14101 ttaagtcgaa cattttccat aaacagatct ttttcccgct gtttagcggc ttccaactcc
    14161 atcgacaatt tttccaactg ctctttatgc ttcttgtatt cctgatacgc gtgccaagac
    14221 tgacctttgc gcacactatc agtgatatca gcaacctgct ccggtgttag cgtggtcagt
    14281 ggctgtgctg gaaaaatcat cactttcccg gcatcccaat caaaacccgc ctgaattgac
    14341 tgaacctcaa ctgatggtgt tgaaccgatg cgaccaggcg aatgaacaac gatcgttaca
    14401 tccatatcgc gacgatggct gtggttgttg gacaaaatac gattcaccaa ctcagaaaat
    14461 ttggaaaatt tcatgctgat ccctctttct ctccccggat aattctgttg cacacatcca
    14521 cacactcgtc gcagatgaaa acggcgtccc cagcaattaa ctttttgacg gcgtactggg
    14581 atttgttgca gaagctgcaa caaagtgttc gcctgactga taaggatgga gcgccagatg
    14641 tcagccgctc catatcgttc ttgcgacgca atatcacacg gctgaactca accagtttct
    14701 ccggggatat ctcctcctcc gtagcaagcg cctctaatcg ttcgagtatg tggaaggctt
    14761 tt
//
LOCUS       DAZOXR010000099        14369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZOXR010000099 DAZOXR010000000
VERSION     DAZOXR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.101"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHUNN_002633"
     CDS             complement(<1..696)
                     /locus_tag="ACHUNN_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0576976.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHUNN_002634"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHUNN_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0576977.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUNN_002635"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUNN_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0576978.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUNN_002636"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUNN_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0576979.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUNN_002637"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUNN_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0576980.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUNN_002638"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUNN_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0576981.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUNN_002639"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUNN_002639"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0576982.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUNN_002640"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUNN_002640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0576983.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUNN_002641"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUNN_002641"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0576984.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHUNN_002642"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHUNN_002642"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0576985.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHUNN_002643"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHUNN_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0576986.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUNN_002644"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUNN_002644"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0576987.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUNN_002645"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUNN_002645"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0576988.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUNN_002646"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUNN_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0576989.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUNN_002647"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUNN_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR0576990.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="ACHUNN_002648"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="ACHUNN_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="ACHUNN_002649"
     CDS             complement(12845..14032)
                     /locus_tag="ACHUNN_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0576991.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14369)
                     /locus_tag="ACHUNN_002650"
     CDS             complement(14032..>14369)
                     /locus_tag="ACHUNN_002650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0576992.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3606 c         3708 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttac
//
LOCUS       DAZOXR010000100        14366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZOXR010000100 DAZOXR010000000
VERSION     DAZOXR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.102"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHUNN_002651"
     CDS             <1..228
                     /locus_tag="ACHUNN_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0576993.1"
                     /translation="LYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAE
                     LLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            384..1919
                     /gene="gadC"
                     /locus_tag="ACHUNN_002652"
     CDS             384..1919
                     /gene="gadC"
                     /locus_tag="ACHUNN_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0576994.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2050..3369
                     /locus_tag="ACHUNN_002653"
     CDS             2050..3369
                     /locus_tag="ACHUNN_002653"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0576995.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3741..5123
                     /gene="dosC"
                     /locus_tag="ACHUNN_002654"
     CDS             3741..5123
                     /gene="dosC"
                     /locus_tag="ACHUNN_002654"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0576996.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5148..7547
                     /gene="dosP"
                     /locus_tag="ACHUNN_002655"
                     /gene_synonym="pdeO"
     CDS             5148..7547
                     /gene="dosP"
                     /locus_tag="ACHUNN_002655"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR0576997.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7806..8387
                     /gene="ddpX"
                     /locus_tag="ACHUNN_002656"
     CDS             7806..8387
                     /gene="ddpX"
                     /locus_tag="ACHUNN_002656"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR0576998.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8401..9951
                     /locus_tag="ACHUNN_002657"
     CDS             8401..9951
                     /locus_tag="ACHUNN_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0576999.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9953..10975
                     /locus_tag="ACHUNN_002658"
     CDS             9953..10975
                     /locus_tag="ACHUNN_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0577000.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10972..11868
                     /gene="ddpC"
                     /locus_tag="ACHUNN_002659"
     CDS             10972..11868
                     /gene="ddpC"
                     /locus_tag="ACHUNN_002659"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0577001.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11865..12851
                     /locus_tag="ACHUNN_002660"
     CDS             11865..12851
                     /locus_tag="ACHUNN_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0577002.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12880..13770
                     /locus_tag="ACHUNN_002661"
     CDS             12880..13770
                     /locus_tag="ACHUNN_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR0577003.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13826..14257)
                     /gene="osmC"
                     /locus_tag="ACHUNN_002662"
     CDS             complement(13826..14257)
                     /gene="osmC"
                     /locus_tag="ACHUNN_002662"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0577004.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3517 a         3570 c         3653 g         3626 t
ORIGIN      
        1 ctgtacgacc tctctgaacg tctgcgtctg cgcggctggc aggttccggc cttcactctc
       61 ggcggtgaag ccaccgacat cgtggtgatg cgcattatgt gtcgtcgcgg cttcgaaatg
      121 gactttgctg aactgttgct ggaagactac aaagcctccc tgaaatatct cagcgatcac
      181 ccgaaactgc agggtattgc ccagcagaac agctttaaac atacctgata acgtttaacg
      241 gtaacggtgt cccgaaacga acccgtttcg ggataatttc caaagtctgt tcactggcat
      301 tagcaacgga aaatattgtt ctgaatacgc ttcagaacaa aacaggtgcg gttccgacag
      361 gaataccgtt ttagggggat aatatggcta catcagtaca gacaggtaaa gctaagcagc
      421 tcacattact cggctttttt gccataacgg catcgatggt aatggctgtt tatgaatacc
      481 ctaccttcgc aacatcgggc ttttcattag tcttcttcct gctattaggc gggattttat
      541 ggtttattcc cgtgggactt tgtgctgcgg aaatggccac cgtcgacggc tgggaagaag
      601 gtggtgtctt cgcctgggta tcaaatactc tggggccgag atggggattt gcagcgatct
      661 catttggcta tctgcaaatc gccattggtt ttattccgat gctctatttc gtgttagggg
      721 cactctccta catcctgaaa tggccagcgc tgaatgaaga ccccattacc aaaactattg
      781 cagcactcat cattctttgg gcgctggcat taacgcagtt tggtggcacg aaatacacgg
      841 cacgaattgc taaagttggc ttcttcgccg gtatcctgtt acctgcattt attttgatcg
      901 cattagcggc tatttatctg cactccggtg cccccgttgc tatcgaaatg gattcaaaga
      961 ccttcttccc tgacttctct aaagtgggca ccctggttgt atttgttgcc ttcattttga
     1021 gttatatggg cgtagaagca tccgcaaccc acgtcaatga aatgagcaac ccagggcgcg
     1081 actatccgtt ggctatgtta ctgctgatgg tagcggcaat ctgcttaagc tctgttggtg
     1141 gtttgtctat tgcgatggtc attccgggta atgaaatcaa cctctccgca ggggtaatgc
     1201 aaacctttac cgttctgatg tcccatgtgg caccggaaat tgagtggacg gttcgcgtga
     1261 tctccgcact gctgttgctg ggtgttctgg cggaaatcgc ctcctggatt gttggtcctt
     1321 ctcgcgggat gtatgtcaca gcgcagaaaa acctgctgcc agcggcattc gctaaaatga
     1381 acaaaaatgg cgtaccggta acgctggtca tttcacagct ggtgattact tctatcgcgt
     1441 tgatcatcct caccaatacc ggtggcggta acaacatgtc cttcctgatc gcactggcgc
     1501 tgacggtggt gatttatctg tgtgcttatt tcatgctgtt tattggctac attgtgttgg
     1561 ttcttaaaca tcctgactta aaacgcacat ttaatatccc tggtggtaaa ggggtgaaac
     1621 tggtcgtggc tattgtcggt ctgctgactt caattatggc gtttattgtt tccttcctgc
     1681 cgccggataa catccagggt gactctaccg atatgtatgt tgaattactg gtcgttagtt
     1741 tcctggtggt acttgccctg ccctttattc tctatgctgt tcatgatcgt aaaggcaaag
     1801 cgaataccgg cgtcactctg gagccaatca acagtcagaa cgcaccaaaa ggtcacttct
     1861 tcctgcaccc gcgtgcacgt tcaccacact atattgtgat gaatgacaag aaacactaac
     1921 catgtctgaa tggttataag acaagggagc gataattcat cgctcccttt ttcgtgctcg
     1981 ctgtcgtatt gacttaaccg ggataagtac gagaatgagc gcacatctgt ttaccggaaa
     2041 ccagcatata tggatatctg ctcccgaaac gagaaattag cgattagaag accagcgata
     2101 ctggttgcac tggcactttt actgtgtagt tgtaaaagca cgcctccaga gtccatggtg
     2161 acaccaccag caggttcaaa gccaccagcc acgacgcaac aatcgtcaca accgatgcgt
     2221 ggcatctggc tggccacggt gtctcgcctc gactggccac cggtttcctc ggttaacatt
     2281 agtaacccca ccagccgggc ccgtgtacaa caacaggcga tgatcgacaa actggatcat
     2341 ctgcaacgtc tcggtataaa cacggtcttt ttccaggtta agccggacgg taccgccctg
     2401 tggccatcga aaattttgcc atggtccgat cttatgaccg gtaagattgg tgaaaatccg
     2461 ggttacgatc cgctgcaatt tatgctcgac gaagcccaca agcgtgggat gaaagtacac
     2521 gcctggttta acccctatcg cgtatcggtt aatacgaagc ccggtactat cagggaactg
     2581 aatagcactc tgtctcaaca accggcgagc gtctatgtgc aacaccgtga ctggatcaga
     2641 acgtctggcg atcgctttgt cctcgacccg ggcatccctg aggttcagga ctggatcaca
     2701 tcaatagtcg cagaagtggt ttcccgctat ccggtagatg gcgtgcagtt tgacgactat
     2761 ttctatactg agtcaccggg ttcacggcta aatgataacg aaacgtaccg caaatacgga
     2821 ggcgcatttg cgtcaaaagc agactggcgg cgcaacaata ctcagcagtt aattgcaaaa
     2881 gtatcgcaca ccattaaaag cattaagccg ggagtcgaat ttggcgttag cccggcaggc
     2941 gtgtggcgta accgatcaca cgatccgctc ggttccgata cccgaggcgc ggcagcctat
     3001 gacgaatcct acgctgacac ccgtcgatgg gtggaacaag gattgctgga ttacattgct
     3061 ccccaaattt actggccgtt ctcacggagt gccgcgcgtt atgacgtgtt ggcaaaatgg
     3121 tgggcggatg tcgttaaacc gactaggacc cgcctgtata tcggtatcgc cttctataaa
     3181 gtgggtgaac cttcaaagat agagccagac tggatgatta acggcggcgt accggaactg
     3241 aaaaagcagc tcgatcttaa cgatgcggta ccagaaatta gcggcaccat cttgttccgt
     3301 gaggactatc tgaataaacc gcagactcaa caagcggtca gctatctgca aagtcgttgg
     3361 ggcagttaaa ctcccatgct tactttgtaa caagccggtg ctttacccac cggcttgtta
     3421 caccttgtga aatatcccac tagtaatcat gcttacataa gtcaaattaa ccacgcaatt
     3481 aaccacactt agttaaacag ctataaatcc ctaataaaaa tgtatgaata tgaaaaacca
     3541 tacgatattc gctgtcaccg aaaaataatt acgatgggct atactcttct gaagaacagt
     3601 agcgtagatt gaaactgtcc taagtcgctt atttcttttc agtatatctt catatttcag
     3661 gagaatatgg tgcagttagt caatctcgca cgttgcgttt tgattccgtc tgatttccca
     3721 gccttataag ggtgtgaatt atggagatgt attttaaaag aatgaaagat gagtggaccg
     3781 gacttgtcga acaggcagat ccactcattc gtgctaaagc cgcggaaatt gccgttgcgc
     3841 atgctcatta tctgagtatc gagttttatc gaattgtccg catcgacccg catgccgaag
     3901 aattcttgag taatgaacaa gttgagcggc agttgaaaag tgcgatggaa cgctggatta
     3961 ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct aatacaaatc cagcataccg
     4021 tcgcggaagt gcatgcccgc ataggaattc cggtagaaat tgtcgagatg gggtttcggg
     4081 tgctgaaaaa aatcctctat ccggtaatct tctcttcgga ttattccgcc gcagaaaaac
     4141 ttcaggtcta tcatttctcg attaacagta ttgatatcgc gatggaagtg atgacccgcg
     4201 cgtttacctt tagtgacagt agtgcctcaa aggaagatga aaattatcgt atcttctcgt
     4261 tactggaaaa cgccgaagaa gaaaaagaac ggcaaatagc ctcaatactt tcatgggaaa
     4321 tagatattat ctataaaatc ctgctggatt ctgatttagg cagtagtttg ccattaagcc
     4381 aggctgattt tggcctgtgg tttaaccata aaggtcgaca ttattttagt ggcattgccg
     4441 aagtaggcca tatctcccgt ctcattcagg atttcgacgg tattttcaat caaaccatgc
     4501 gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa atttttatta cagataagaa
     4561 ataccgtatc gcagattatt accttgctgc gtgaattgtt tgaagaagta tcgcgccacg
     4621 aagtcggtat ggatgtacta acgaaattac ttaaccgccg tttcctgccg actatcttca
     4681 aacgcgaaat tgcccatgcc aaccggaccg gtacaccgct gtcagtgctg attattgacg
     4741 ttgataaatt caaagagatc aacgatacgt ggggccataa cactggcgat gaaattctgc
     4801 gtaaagtctc tcaggccttt tatgacaacg tccacagtag tgattatgtt tttcgctacg
     4861 ggggcgatga atttatcatt gttttgactg aagcttctga aaacgaaacg ttacgtaccg
     4921 cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa agccgcaaac ggcgaggata
     4981 ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc
     5041 tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga aaccgtgttg
     5101 aactctggaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg
     5161 atgcggaaaa tgccgccgat ggcatttttt tccccgccct tgaacaaaat atgatgggcg
     5221 cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca gagaagctct
     5281 gggggtacaa acgtgaagaa gtcattggca ataacattga tatgctgatt ccacgggatt
     5341 tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga aggtggtaaa gcgcgcgtcg
     5401 aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc tggacccgtt
     5461 ttgcgctatc gaaagtgagc gccgagggga aagtttatta cctggcgctg gtacgggatg
     5521 ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc gttgaccatc
     5581 tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat
     5641 ttaccgaaat gtttggttac tgcattagcg aagccagcgg tatgcagccc gatacactcc
     5701 tgaacattcc tgaattccct gccgataacc gcattcgttt acaacagttg ctatggaaaa
     5761 ccgcccgcga tcaggacgaa tttctgctgt tgacacgcac cggtgaaaaa atctggatta
     5821 aagcctctat cagcccggtt tatgacgtgc tcgcgcatct gcagaacctg gtaatgacct
     5881 tctcggatat caccgaagaa cggcagatcc gccagcttga aggcaatatt ctcgccgcca
     5941 tgtgcagcag cccgccattt catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg
     6001 ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa cgggatgccg atacactggg
     6061 cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc
     6121 agcgtgatgg tgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga gcagaaacca
     6181 gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg gcgctggaac
     6241 aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccgatg accggtctgc
     6301 caaatcgcaa taacctgcac aattacctcg atgaccttgt cgacaaagcc gtctctccgg
     6361 tggtgtatct catcggtgtt gaccatattc aggatgtgat tgatagcctt ggctatgcgt
     6421 gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc aaaccggatc
     6481 agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt gagcctggaa aacgacgtca
     6541 gtaacattac ccaaatcgcc gatgagctac ggaatgtggt cagcaagccg ataatgattg
     6601 acgataaacc attcccgcta accttgagta ttggcatcag ctacgacgtg ggtaaaaacc
     6661 gcgattacct gctctccact gctcacaatg caatggatta tattcgcaag aatggcggta
     6721 acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt ttggttttag
     6781 gcgcagcgct gaaagaagcg attagcaata accaactgaa actggtttac cagccgcaaa
     6841 tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct tgctcgctgg cacgatcccc
     6901 agcatggtca tgtaccccct tcacggttta ttcctctcgc agaagagatt ggtgaaatcg
     6961 aaaatattgg gcgctgggtc atcgcggaag cttgccgtca gttagcagaa tggcgtagcc
     7021 agaatattca tatcccggcg ttatccgtga acttgtcggc gctgcacttt cgcagtaatc
     7081 agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg tattgacggc caccagctga
     7141 cggtagaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt aagcgcattc
     7201 agatcctgcg cgatatgggc gtgggcttat cggtagatga ttttggcacg ggcttttccg
     7261 gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgacaaa agttttgtcg
     7321 atcgttgtct gacggaaaaa cgcatccttg ccttacttga agccattacc agcattgggc
     7381 aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac caaagagcaa tttgagatgc
     7441 tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc ctacccgccg
     7501 aggaaattcc aggctggatg agcagcgtgt taccgctgaa aatctgacaa attcctctcg
     7561 cccgcactcg cgggttttca tttaacgtga cgctgtcact taaatactgt gatttcagct
     7621 acgattccgg gagattcctt ccttaacttc ccctcttgca ccaaaactgc actacgctgc
     7681 acaaatgttg atcaaaagtt aaaaaatata actttaagcc agcattctgg cgcttatccc
     7741 ggcatggcat gagatctgca taagcggaaa gcgcagcaat ttttgtctta atacaggaac
     7801 cgtttatgtc ggataccacc gaactggttg atttagccgt gatcttccct gatctggaga
     7861 tcgaattgaa atacgcctgc gctgataaca tcacaggtaa agctatttac cagcaagcgc
     7921 gttgtctgtt acacaaggat gcgattaccg cgctggcgaa aagtatcagt atcgcccagc
     7981 tgtcaggctt acaactggtg atttacgatg cgtatcgccc acaacaagca caggcgatgt
     8041 tgtggcaagc ctgcccagac ccgcaatatg ttgttgatgt gacggtcggt tctaatcaca
     8101 gccgtggcac ggcgatcgac ctgacgcttc gtgatgagca cgggaacatc ctggatatgg
     8161 gcgcagggtt cgatgaaatg cacgagcgct cccatgccta tcacccttcc gtcccgcccg
     8221 ccgctcagcg caatcgcctg ttgctgaatg cgataatgac tggtggcggt tttgtcggta
     8281 tctccagcga atggtggcac ttcgaattac cccaggcagc gagttaccct ctgctcgctg
     8341 atcaattctc ttgttttata tcccctggca cacagcacgt cagttaatca ggaaactgtc
     8401 atgaagagat cgatatcgtt tcgtcccaca ttgctcgcgc tcgtccttgc cacaactttc
     8461 ccggttgcgc acgccgccgt accgaaagat atgctggtga ttggtaaggc cgccgatcca
     8521 caaaccctcg acccggcggt aacaatagat aataacgact ggacagtgac ctacccgtct
     8581 tatcagcggc tggttcagta caaaacggac ggtgataaag gctcaaccga cgttgaaggc
     8641 gatctggcaa gtagctggaa agcgtctgac gatcaaaaag agtggacgtt caccctgaag
     8701 gacaatgcta aatttgccga tggcacacct gtcactgccg aagcagtaaa actctctttt
     8761 gagcggttac taaaaatcgg ccaggggcca gcagaagcat ttcccaaaga tttaaagatt
     8821 gatactcccg acgaacatac ggtgaagttt acccttagcc aaccattcgc accgttcctc
     8881 tacacgctag cgaatgacgg tgcatccatt atcaatccgg cggtcttaaa ggaacatgca
     8941 gcggatgatg ctcgcggctt cctcgcgcaa aataccgccg gttccggacc atttatgctg
     9001 aaaagcgggc aaaaaggtca gcaattagtt ctggtgccaa atccgcatta ccccggcaat
     9061 aaaccgaact ttaagcgagt atcggtaaaa attatcggtg aaagtgcctc ccgtcgcctg
     9121 cagctctccc gtggcgacat tgacattgcc gatgcgctgc cggtggatca actcaacgcc
     9181 ctgaagcagg aaaataaagt caatgtggca gagtatccgt cactgcgcgt tacctatctg
     9241 tatctgaata acagcaaagc gcctcttaat caggcggatc tgcgtcgggc catttcctgg
     9301 tctaccgatt atcagggcat ggttaacggc attctgagtg gtaacggaaa acagatgcgc
     9361 ggcccgattc cggaaggcat gtggggctac gatgcgacgg caatgcaata caaccatgac
     9421 gaaacgaaag ccaaagccga atgggataaa gtgacgagca aacccaccag cctgacgttt
     9481 ctctactccg ataacgatcc gaactgggag cctattgctc tggcgacaca atccagtctc
     9541 aacaagctgg gcatcaatgt gaagctggaa aagctggcga acgccaccat gcgcgacaga
     9601 gtgggtaaag gtgattacga cattgcgatt ggcaactgga gtccggattt tgccgacccg
     9661 tatatgttta tgaattactg gtttgagtca gacaaaaaag gtctgccggg taaccgctcg
     9721 ttctatgaaa acagtgaggt cgataagtta ctgcgcaatg cgcttgcgac caccgaccag
     9781 acgcagcgta cccgggacta ccagcaggca cagaaaatcg tcattgatga cgctgcttat
     9841 gtgtacctgt tccagaaaaa ctaccaactg gcgatgaaca aagaggtgaa aggctttgtg
     9901 ttcaatccca tgctggaaca ggtcttcaat atcaatacca tgagtaaata acgtgacctt
     9961 ctggagtatt ttacgccaac gctgctgggg gctggtgctc gtggtggcgg gcgtctgcgt
    10021 gattacgttt attatctcgc atctgatccc tggcgatccg gcgcggttac tggcgggtga
    10081 ccgcgccagc gatgctatcg tggaaaatat tcgccagcaa ctgggactgg accagccact
    10141 gtatgtacag ttttaccgct acttcagcga cctgtttcat ggtgacctgg gaacatccat
    10201 tcgtaccggg cgtccggtgc tggaagagtt gcgtatattt ttcccggcga cgctggaact
    10261 ggctttttgt gccctgctgc tggcactcct gattggcatc ccgctgggca tactctctgc
    10321 agtctggcga aatcgctggc tggatcatct ggtgcgaata atggccatta ccggaatctc
    10381 cacacctgcg ttctggcttg gactgggcgt cattgtgctg ttttatggtc atctgcaaat
    10441 tcttcccggc ggcggaaggc ttgatgactg gctggatcca ccaacgcacg ttaccggctt
    10501 ttatctgctc gatgcgctgc ttgaaggcaa cggtgaagtc ttcttcaatg cgttgcaaca
    10561 tctcatctta ccggcattaa cgctggcgtt cgttcacctg ggaattgtcg ctcgccagat
    10621 ccgctcagcg atgctggaac aattgagtga agactacatt cgtaccgccc gggccagcgg
    10681 cttgcccggc tggtatatcg ttttatgtta tgcgctaccc aatgcgttga tcccatcgat
    10741 taccgtattg ggtctggcgc tgggcgattt gttgtatggc gcagtgctca ccgaaaccgt
    10801 ttttgcctgg cccggaatgg gtgcatgggt agtaacatca atacaggcgc tcgacttccc
    10861 ggcagtgatg ggctttgccg tcgtggtttc atttgcttat gtgctggtca acctggtggt
    10921 ggatttgctc tatttgtgga ttgatccgcg tatcggacgt ggaggtggtg aatgatgcta
    10981 agcgaggaaa cgtccgccgt acgcccacaa aaacaaacgc gatttaacgg tgcaaaactg
    11041 gtatggatgc tgaaaggcag tccgctcacc gtgaccggcg cagtcatcat tgtattaatg
    11101 ctattgatga tgattttttc accgtggctt gcgacgcatg atcccaacgc cattgattta
    11161 accgcccgcc ttttgccgcc ttctgcggcg cactggtttg gcaccgatga agtgggacgc
    11221 gatctgttca gccgcgtact ggtcggcagt cagcaatcaa ttctcgccgg attagtcgtg
    11281 gttgccattg cgggtatgat tggttcgcta ctcggatgtc tatccggtgt gcttggcgga
    11341 cgcgcagacg ccatcatcat gcgcatcatg gacattatgc tgtcgattcc ttcgttggta
    11401 ctgacaatgg cactggctgc cgctctcggg ccgagtttat ttaacgccat gctggcgatt
    11461 gctattgtgc gaattccctt ttatgtgcgc ctggcgcggg ggcaagcatt agttgtacgc
    11521 cagtatacct atgttcaggc ggcgaaaacc tttggtgcgt cgcgttggca tctgatcagc
    11581 tggcatattt tacgtaactc cctgccgccg ctgatcgtgc aggcatcgct ggatatcggt
    11641 agcgcgattt taatggccgc cacgttggga tttattggcc tgggtgctca acaaccgagt
    11701 gctgaatggg gcgcgatggt agctaatggt cgcaactatg tactcgatca gtggtggtat
    11761 tgcgcatttc cgggggcagc gattttgctt accgccgtcg ggtttaatct ctttggtgat
    11821 ggtattcgcg atctgcttga cccgaaagca ggaggaaagc agtcatgacc caacccgttc
    11881 tggacattca acagctgcat ttgagtttcc ccggttttaa cggttatgtt cacgtgctca
    11941 acaatgtgtc cttgcagatt aaccgcggtg aaattgtcgg tctggtggga gaatccggct
    12001 caggtaaatc agtcaccgca atgctgatta tgcgtctgct accgacgggc agttattgcg
    12061 tacatcgggg gcagatttca ctgctgggag acgatgttct taacgctcgg gagaaacagc
    12121 ttcgtcagtg gcgcggcgca cgagtggcga tgatctttca ggaaccgatg accgccctca
    12181 atccgacacg tcgaataggt cttcagatga tggacgtgat ccgccatcat caaccaataa
    12241 gtcgtcggga agccagagct aaagcgatta ccctgctgga agagatgcaa atcccggatg
    12301 ccgtggaagt tatgtcgcgc tatccgtttg agctttcagg tggtatgcgc cagcgggtaa
    12361 tgattgcgct ggcattctcc tgcgagccgc aattgattat tgccgacgaa ccgactacgg
    12421 cgctggacgt cacggtacag ttgcaggtac tgcgtttgct taaacataaa gctcgcgcca
    12481 gtggaactgc ggtactgttc atcagccatg atatggccgt ggtgtcgcaa ctgtgcgata
    12541 gcgtttacgt gatgtatgcc ggaagcgtaa ttgaaagcgg cgtgacggca gacgttatcc
    12601 atcatccccg gcatccgtat accattggtt tgctgcaatg cgcaccagaa catggagtac
    12661 cacgccagcc attacccgcc attccaggga cggtaccaaa cctcacccat ttgcctgacg
    12721 gctgcgcttt tcgcgatcgt tgctatgcgg caggtgcaca gtgtgaaaac gtcccggcgc
    12781 tgacagcgtg tggtgacaac aaccagcgct gcgcctgttg gtatccgcag caggaggtca
    12841 taagtatctg acacgttatt aacgttacgc gacgtccata tcaatttccc ggcccgtaaa
    12901 agctggcttg gtaaaactac ggaacatgtt catgcgatta atggtattga tttacagatc
    12961 cgccgtggtg aaaccttagg gatcgtcggt gagtcaggct gcggcaaaag caccctcgca
    13021 cagcttttaa tgggtatgct gcaaccgagc cacgggcaat acatccgttc aggatcacaa
    13081 cgcattatgc agatggtgtt tcaggacccg ctctcttcgc ttaatccgcg cttatcggtg
    13141 tggcgcatca tcacagaacc gctctggata gctaagcgta gtagtgaaca acagcggcga
    13201 gcgttggcaa aggagttggc tgtgcaggtg ggtattcgtc cggagtatct cgaccgcctg
    13261 cctcatgcgt tctccggcgg gcagcggcaa cgcatcgcca ttgccagagc actctcttcg
    13321 cagcctgacg tgattgtgct tgatgagcca acctcggcgc tggatatctc cgtgcaggcg
    13381 cagatcctca atttactggt aacgctacag gaaaatcacg ggctgactta tgtgctgatt
    13441 tcacacaatg tctcggtgat acgtcatatg agcgatcggg tggcggtgat gtatctcggg
    13501 cagattgtag aactgggcga cgcgcagcag gtgctgacgg cacctgcaca tccatacacc
    13561 cgattattgc tggattccct ccccgccatt gataaaccgc tggaggaaga atgggcatta
    13621 tgtaaaacgg atctgccggg aaaccgcacg ttgccacaag gctgtttttt ctacgaacgt
    13681 tgccctctgg caacccacgg atgtgaagtc cggcaatcat tagcaataag ggaggacgga
    13741 cgtgagctcc ggtgctggcg ggcgctgtag agatgctaac aacgcatcag gcattcacgc
    13801 gcctgacgcg tcatccggca atgctttacg atttcaactg gtaatccagc gtaatttccg
    13861 ctttcagtac ctgagaaacc gggcatcctg cttttgcttt ctggatgatg ccgtcaaagg
    13921 tagaggcatc aatacccggc accgcaactt cactcttcag tgcgattttc gtaatcgcaa
    13981 aatcggcatc cactttatcc agcgacacat cggcggtggt atcaatcgat gttggcgtga
    14041 atcccgcttc ccccagcatt aatgaaagcg ccattgagaa acatgcggca tgcgctgcgc
    14101 caatcagttc ttcagggttg gttccttttt cgccttcaaa acgcgtgtta aatccatacg
    14161 gctgttggtt cagcacgcca ctctgggtgg atactgttcc cttcccgcgt ttgatatcgc
    14221 cttcccagtg tgcctgacct ttcttatgga ttgtcattgt tgctctcctg tgggcttaaa
    14281 aaggaaatta tagactacgt gagaaacagc acgaggataa gcaggatatt ccgaataaaa
    14341 tacggcgaaa atattacttc tgcact
//
LOCUS       DAZOXR010000101        14312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZOXR010000101 DAZOXR010000000
VERSION     DAZOXR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.103"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUNN_002663"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUNN_002663"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0577005.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHUNN_002664"
     CDS             2273..2662
                     /locus_tag="ACHUNN_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0577006.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUNN_002665"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUNN_002665"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0577007.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUNN_002666"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUNN_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0577008.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHUNN_002667"
     CDS             complement(4321..4521)
                     /locus_tag="ACHUNN_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0577009.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHUNN_002668"
     CDS             4687..5232
                     /locus_tag="ACHUNN_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0577010.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUNN_002669"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUNN_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUNN_002670"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUNN_002670"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0577011.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHUNN_002671"
     CDS             complement(6529..7353)
                     /locus_tag="ACHUNN_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0577012.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUNN_002672"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUNN_002672"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0577013.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUNN_002673"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUNN_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0577014.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUNN_002674"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUNN_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0577015.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUNN_002675"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUNN_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0577016.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHUNN_002676"
     CDS             complement(11316..11969)
                     /locus_tag="ACHUNN_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0577017.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUNN_002677"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUNN_002677"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0577018.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUNN_002678"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUNN_002678"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0577019.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            14120..>14312
                     /locus_tag="ACHUNN_002679"
     rRNA            14120..>14312
                     /locus_tag="ACHUNN_002679"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3496 a         3603 c         3801 g         3412 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aggtttaatt
    14101 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
    14161 gcaggcctaa cacatgcaag tcgaacggta acaggaaaca gcttgctgtt tcgctgacga
    14221 gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa
    14281 acggtagcta ataccgcata atgtcgcaag ac
//
LOCUS       DAZOXR010000102        14306 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZOXR010000102 DAZOXR010000000
VERSION     DAZOXR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.104"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="ACHUNN_002680"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="ACHUNN_002680"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0577020.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHUNN_002681"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHUNN_002681"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0577021.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHUNN_002682"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHUNN_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR0577022.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="ACHUNN_002683"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="ACHUNN_002683"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0577023.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="ACHUNN_002684"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="ACHUNN_002684"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0577024.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHUNN_002685"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHUNN_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0577025.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHUNN_002686"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHUNN_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0577026.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHUNN_002687"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHUNN_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0577027.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="ACHUNN_002688"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="ACHUNN_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0577028.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="ACHUNN_002689"
     CDS             complement(13987..>14306)
                     /locus_tag="ACHUNN_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0577029.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAZOXR010000103        14299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZOXR010000103 DAZOXR010000000
VERSION     DAZOXR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.105"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            42..398
                     /locus_tag="ACHUNN_002690"
     CDS             42..398
                     /locus_tag="ACHUNN_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0577030.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(449..1345)
                     /gene="yhaJ"
                     /locus_tag="ACHUNN_002691"
                     /pseudo
     CDS             complement(449..1345)
                     /gene="yhaJ"
                     /locus_tag="ACHUNN_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1450..2151
                     /locus_tag="ACHUNN_002692"
     CDS             1450..2151
                     /locus_tag="ACHUNN_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0577031.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2174..2338
                     /gene="yhaL"
                     /locus_tag="ACHUNN_002693"
     CDS             2174..2338
                     /gene="yhaL"
                     /locus_tag="ACHUNN_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0577032.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2472..3782)
                     /gene="cyuA"
                     /locus_tag="ACHUNN_002694"
     CDS             complement(2472..3782)
                     /gene="cyuA"
                     /locus_tag="ACHUNN_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0577033.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3810..5141)
                     /locus_tag="ACHUNN_002695"
     CDS             complement(3810..5141)
                     /locus_tag="ACHUNN_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0577034.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5416..6780)
                     /gene="tdcG"
                     /locus_tag="ACHUNN_002696"
     CDS             complement(5416..6780)
                     /gene="tdcG"
                     /locus_tag="ACHUNN_002696"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0577035.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6852..7241)
                     /locus_tag="ACHUNN_002697"
     CDS             complement(6852..7241)
                     /locus_tag="ACHUNN_002697"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0577036.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7255..9549)
                     /gene="tdcE"
                     /locus_tag="ACHUNN_002698"
     CDS             complement(7255..9549)
                     /gene="tdcE"
                     /locus_tag="ACHUNN_002698"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0577037.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9583..10791)
                     /gene="tdcD"
                     /locus_tag="ACHUNN_002699"
     CDS             complement(9583..10791)
                     /gene="tdcD"
                     /locus_tag="ACHUNN_002699"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0577038.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10817..12148)
                     /gene="tdcC"
                     /locus_tag="ACHUNN_002700"
     CDS             complement(10817..12148)
                     /gene="tdcC"
                     /locus_tag="ACHUNN_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0577039.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12170..13159)
                     /gene="tdcB"
                     /locus_tag="ACHUNN_002701"
     CDS             complement(12170..13159)
                     /gene="tdcB"
                     /locus_tag="ACHUNN_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0577040.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13258..14196)
                     /gene="tdcA"
                     /locus_tag="ACHUNN_002702"
     CDS             complement(13258..14196)
                     /gene="tdcA"
                     /locus_tag="ACHUNN_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0577041.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3685 a         3839 c         3345 g         3430 t
ORIGIN      
        1 cggaatagac tttcgtcact tttaattaaa gggatgtttt tatgcagtgg tatttatccg
       61 tattaaaaaa ttatgttggt ttctctggtc gcgcacgtcg taaagagtac tggatgttta
      121 ctctgattaa cgccatcgtc ggcgctatta tcaatgtcat tcaattgatt ttaggtctgg
      181 agcttccata tctgtctatg ctttatttgc tggcaacctt ccttcccgtc cttgcgcttg
      241 ctatccgtcg cttacacgat accgaccgtt caggtgcctg ggccttgctc ttttttgttc
      301 cgtttattgg ctggctcgtg cttctggttt ttttctgcac agaaggtact tctggcagca
      361 atcgttacgg aaacgatccg aagtttggtt caaattaatc ttagaattgg ggcgatattt
      421 cgcccctttt tattaacaat aataaatatt attttccgct aaaaagtttg ggaatttccc
      481 gcagacacca ggatttcgct tcccccatac tgtcacgacg ccaggccata ataatatcga
      541 tctcactggt cgattccggg ctgacgacac gcaaccgccc ttccgcaata tctttttcga
      601 ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga cgtttatctt
      661 caatcgtgct caccgttaag cgcggctgtt tgtccagcag ttgtacggtc aacaccgggc
      721 gctcacgagc ggtatccgct accgcaattc cacgatattt cacgcgcgtc acttcagata
      781 acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg ttcattaacg
      841 tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc ggcgcgataa
      901 caatatccgc ccgcccctgc tccagtcgtt cctacgcccc cgccagcact tcggtgatga
      961 ttgccagttg ggtattggct tttgccgcca gtttgtcgat taacgggaaa aaggcaggtg
     1021 tcggtaccag cgcttcggtc acaatggtga gatgcgtttc ccaaccgcgc gcgagggctt
     1081 ccgcatcggt agtcagttta tctgcggctt ccagcaaaac gcgccctcgc tccagcaaca
     1141 tcctcccgac attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc accacatcca
     1201 gctcttcttc cagtttttgc atggtgtagc taagtgcgga aggcacgcgt cccagctcat
     1261 ccgccgccgc cgcaaaactg ccccggcgat cgatcgcatc cataacccgt agtgcttcca
     1321 gcgttaatgc cctttctttg gccatttcgt tctcattcag gaaatttgaa cataccgggc
     1381 agaatatctg gctaacaatg tagcgtccag ccccttacca taaaaggaag taaagagagg
     1441 tcaagaatta tgattactac ccgaactgcc aggcagtgtg gacaagcaga ctacggatgg
     1501 ttgcaggccc ggtatacttt ttcctttgga cactacttcg acccgaaatt gttaggctat
     1561 gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag gtgccgcctt tcagccgcga
     1621 acctatccca aagtcgatat tttaaatgtg attctggatg gagaagcaga gtatcgcgac
     1681 agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt tgctgctctc tacccagccg
     1741 ggtgtcagct acagcgaaca caatctcagc aaagacaaac cgttaacacg aatgcagctt
     1801 tggctggacg cctgcccgca gcgagagaat ccgctgattc aaaaactggc gcttaatatg
     1861 ggcaagcagc aattaatcgc ctcgccagag ggtacgatgg gaagcctgca attacgccag
     1921 caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt ccagttgcat
     1981 gggccacgcg cgtatttgca atcgattcac gggaaatttc atgcgcttac gcatcatgaa
     2041 gagaaagcgg cgctgacctg cggtgatggg gcgtttattc gtgacgaggc taacattacg
     2101 ctggttgccg attccccact gcgcgctttg ctgatagatt tgcctgtcta gttgttttta
     2161 cagggagatg atgatgagta aaaaatcggc caaaaagcgc cagccagtga agcccgtggt
     2221 ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat gaagagatgt tgagcgagct
     2281 ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta ccgcgtaaag
     2341 acaggttgcc ggatgcggcg aaaacgcctt atccggccta caaaatccaa caacattaac
     2401 gaattaaaca acccgtaggc cggataaggt gcgccagcat cgcatccggc acaatactca
     2461 aaacctggcg tttatctggc cttgctcgcc ataatctcga taatctgccg atccgtttgc
     2521 tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg actgctcaac atcatgcgcc
     2581 acgatccctt cattgccggt cacggcggta tcatccagcg ccattaacac cgctttccac
     2641 gcagccgaag cactggtcga aaccttcatc gcgcagctgt tcgacgcacc atcgcaaatc
     2701 atgccgctga catcgccgat catactgctg atcgccatcg agatagtttc ataacgccca
     2761 tccaccagcc atgccatccc ggcggcggcc cccattgctg cggtcgttgc ggcacaaagt
     2821 gcagacaaac gcggtaactg gttatggatg taaattgcgc tcaaatgaga aagcatcagc
     2881 gcacgcgcca gtcgttcatc atccgctccg aagtgttctg ctacaaccac cacaggcatt
     2941 gttgcggtga tcccctggtt acccgagccg gagttactca tagccggaag cgtagcgccg
     3001 cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga gagatctttc
     3061 gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc caatatgcag cccccactta
     3121 ccgctcaaac cttcctgcga taacgcacaa tttagcttcg cggaatcgag aataaagcgg
     3181 atcgccgcaa acgggacttc attgacgaac ttcaggatct cagccagcgt cgttctggaa
     3241 agcaccgtca gcggagactc ttgctcgccc tctgccacac acgcctgctg ggtaaacacc
     3301 acaccattgt gcgtctcaat acgcacaatg ttggtatgcc cgccgacgat ggtgacacac
     3361 gcccacttct caccgttcca gactttggcg cgtgagaaga ggatttcatt gcaaggttcc
     3421 tggatcttaa cggagacttt ccccgccgcc agcagtgctt tggcatcggc aattgcctgc
     3481 gcagttgcgt ctttcagcac ttccagcccg gcgttggcat ttccacctaa cgcccccagc
     3541 gccgccgcaa tcggcagccc caccattccc gtgccgggaa cggtgacgcc cagaccgttc
     3601 ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc cagttctgcc
     3661 gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacatcccag cgccggtttt
     3721 acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt cgaatcaaac
     3781 atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacgg agaaacacaa
     3841 agcaacaaac cagtgacgat aatcaggtac agagacatcc ctttgtattt gtgcaatgcc
     3901 ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa aatcgggcta
     3961 cagatagagg tgaagctcaa caccggtgcg ttcagtacga tagcgctcca ggccagcaaa
     4021 atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt taatcttctc ggcaggcatc
     4081 ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc taaatagacg
     4141 ccaaagaatg cagtcatgac agcaaagata ttgagaatga cgctgaccac tttcacccac
     4201 gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca gcgcggaaat attctgctcg
     4261 taggctttta ccgcttcgtc atgccccatc gccagcgtga acgacacggc gtagaaaaag
     4321 acggtgacaa acaaaatgcc aaacgcgata ttcattgccc gcaatgcttt atgccgcgcc
     4381 acttcaatag atttttcccg cgagcgataa gagatcacca tcgggcttaa cgtctggata
     4441 aacaaaatcg acgtcagggt aaacggcagc gtaataatgg cgtttttcac cagcagcccc
     4501 agcggcggta gcgaaccgac gttatacaga tgccacattc ccaccatcga cacgcccagc
     4561 gccgcgacca ccagcagctt ggtcagcacc atgccggtcg aaattttgaa taacaatttc
     4621 tcgccgcgtg aagagatcgc caccagaatg caaatcagta ccagaccata aaagggactg
     4681 tctgacagca acccttccgt cacgccgaag gtatgcagat aggaagcact atcgttggtg
     4741 atggcggtgg aatagacgaa catccaaatc accagcatta cgaaatagag cgcacctaac
     4801 aggatgcccc agtttttacc taaataaccg ctaatgacac tcgggtaatc tttacattct
     4861 ggtgattctg ccagcgtatt aataaacaac cgctgaaaca gatacattgc cgggtaacca
     4921 atcaccgatg agagcaaaaa tacccacaat cccatcaaac cgacctgcac cgggagaaaa
     4981 acaatccccg cgccaatcgc catcccgata ctcataatca cccagccggt gtcagtactg
     5041 tcgaatttga tcgcttctcg ccactcgctc tcactcattc cagcacgacc cgccggggtc
     5101 gaagcgtctg caatgacgcc tttattcgat gcgatttcca taatttctcg ctcaatattt
     5161 tgtagggctt attatttttt tccgagccgc atcaaggcga tatgcggtct acgtttttgc
     5221 aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc agagaaaatc ttcacaatca
     5281 agactttgaa ataacggaga tggataagaa ttttctacta aattaatcgc aagagaatat
     5341 tcattctcta tttgcggcag atcacaaaaa aaaggtgcac atttgtgcac ccaaggatga
     5401 aagctgacag caatgtcagc cgcagaccac tttaatggcc agtcctccgc gtgatgtttc
     5461 gcggtattta tcgttcatat ctttgccggt ttcatacatc gtctcgatca ctttatcgag
     5521 tgaaacacgc ggtgccgagg tgcggcgcat cgccatccac gcggcgttta ctgctttcac
     5581 ggcattaatg gcattacgtt caatgcacgg gatttgtacc tgtccggcaa ccggatcgca
     5641 ggtcagccca aggttatgct ccatcgcgat ttccgccgca ttgcatacct gcgccggact
     5701 gccgcccagt agttcagtta accctgccgc cgccattgaa caggccacgc caatctcccc
     5761 ctgacagccg acctccgcgc cagagatgga ggcgttcatt ttatacagcg cgccaatagc
     5821 ccccgctgcc agaaaatagc gggcaattga ccgctcgttt accggacgac ggaacttatc
     5881 gtaataagcc agcactgccg gaataatgcc gcacgcaccg ttagtcggtg ccgttaccac
     5941 gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg tacatgttga tccagtcgat
     6001 gacattcatc ggatcgttag agatgttatc gctagaaacc agctgacgac gcagcgctac
     6061 ggcacgacgc ggcacattga gcggaccagg cagcacgcct tcagtgttca tcccgcgttc
     6121 aataccgtca tgcatcactt gccagatacg ggcaaatcct gcgtcaattt ccgctttgct
     6181 gcgcagcgcc agctcgttgt gcatcatcag gccagatata gacaggccgt tgtaatcaca
     6241 cattttcagc agttcacctg ctgagtggaa atcgtaaggt acggacgttt cgacatcgtg
     6301 cgacaggccg aagtgttctt cttcgacaat aaacccgccg ccgacagagt aataggtttt
     6361 acttaatagc gcttcctgtc ccttccaggc agtgatccgc attccgttct catggcgagg
     6421 caacatttcg ggatggaaga taatgttctt tgctacagga aaatcaacaa tatgcgcacc
     6481 agatgccact ggcagccgcc cgctgcgcgt tactaactct ataaatgcag ggatctcatc
     6541 aatgacaaca tcctgcggac tgtttcctgc cagtcccatg ataatggcga catccgtggc
     6601 atggcctttg cccgtcagtg acaacgaccc atacagatcg accacaatat ggctcgtcgc
     6661 ggttaataag ccgctacttt ccagccgatc aataaaactt tttccggcat tcattggccc
     6721 cacggtatgc gaactggagg gaccaatccc aattttgaaa atatcgaatg cactaatcat
     6781 atccacaccc tcggattgcc gttcagtgaa gtggagcgga acgaccttgc gaccgtcccg
     6841 ctcacgaggc tttacgcact acgtactgcg atagcttcaa tttccagctt cacatctttc
     6901 ggcaaacgcg caacctgcac acagctccgg gtcgggtagg tcgcctgatg ctcatcgaag
     6961 aactgcttat agacttcatt gatggtggca aaatcattca gatcggtgat gaacacggtc
     7021 atcttgatga tatcgcccac gctcagcccg gcagcaacca cgatcgcttt gacgttttcg
     7081 aggcttaaac acgcctgatc ttgcacatca gccgggatct caccggtctg cgggcaaacc
     7141 ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa cgccctgaac ataagggccg
     7201 attgcgcctg gggcacgttg cgtttcgata atctttttca tacatcctcc ggcgtcagag
     7261 cgcctgggta aaagtacgtg aaataacatc ctgttgctgt tcacgggtca gtgcgttgaa
     7321 gcgcacggcg tagccagaga cacggattgt caggttagga tatttttccg ggtgctcgat
     7381 ggcatccagc agcatttccc gattcattac gttgacgttg aggtgttgac cgccttcgac
     7441 atccgcttcg tggtggaaat acccatccag caggccgaca aggttggttt tacgtactgg
     7501 atcttctttg cccagcgccg caggaacgat tgagaaggtg tacgagatcc catctttggc
     7561 gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc acggcacctt tgcggtcacg
     7621 accatgcatc gggttagcgc ccggcgcgaa cggtgttccg gcgcgacgac cgtccggcgt
     7681 attaccggtt ttctggccgt acaccacgtt agaagtgata gtcagaatcg actgggtagg
     7741 gacggcgttg cgataggttg gcagcgcttt aattttcttc ataaagcgtt caaccaggtc
     7801 gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac tgcggatatt caccgtcgat
     7861 ttcaaagtcc accgccaggc cgttttcgtc acggattggt ttcacgcggg catatttgat
     7921 ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg ccgcatgcca tagtgcgata
     7981 gacatcacga tcgtgcagcg ccatcagcga agcttcgtag ctgtacttgt cgtgcatgta
     8041 gtgaatgata ttcagcgcgc tgatgtactg caccgccagc cagtccatga agtgatcgag
     8101 gctgtccatc actttgtcgt aatccagcac gtcgtccatc agcggtgctg ttttcggccc
     8161 gacctgaatc ttcagcttct cgtccacccc gccgttaatt gcgtacagca gtgttttcgc
     8221 caggttagcg cgtgcaccaa agaactgcat ttgcttacca atcaccattg ggctgacgca
     8281 acaagcaatt gcgtaatcgt cgctgttgaa gtcagtacgc atcagatcgt cgttttcata
     8341 ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat tttttgaagg caatcggtaa
     8401 ttcttccgac caaagaatgg tcaggttagg ttccggtgcc ggccccatag tgtgcagggt
     8461 gtgcaaatag cggaaggagt ttttggtcac cagcgtacga ccgtccagcc ccatcccgcc
     8521 gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc gaatcaaatt ccggcgtacg
     8581 caagaagcgc accatacgga tcttcataat gaagtgatcg atcagttcct gtgcctgctg
     8641 ctcattgagt acgcctgctt taaagtcgcg ctcaatgtag atatcgagga acgacgccgt
     8701 gcgccccagc gacatcgcgc cgccgttctg tgatttcact gccgccagat aagcgaagta
     8761 gagccactgc accgcttcct gcgcattctg cgccgggcga gagatatcaa agccatattt
     8821 cgccgccatt tcctgaatct gcaacagcgc atgacgatgc tctgccagct cctcacgcag
     8881 acggatggtg gcttccagat cttcgccttt ttccagacgg gactggagat cggcaaattg
     8941 cagttcgcgt tcacgcacca gataactgat gccatacagc gctacgcggc gatagtcacc
     9001 gataatgcgc ccacggccat agccatctgg taaaccggtc agcacgccag atttacggca
     9061 gcgcagcata tccggtgagt aaacatcaaa tacgccctgg ttatgggttt tacgcagatc
     9121 ggtaaacaga tattcaaatt cactgtccat ttcacggcca taggcgtgga atgaactttt
     9181 aatcatatta atgccaccga acggatgcag cgcgcgcttt aatggcgcat ccgtttgcag
     9241 accaacaatt ttttccagcg gctggttaat atatcccgca tcatgagcgg taattgtggt
     9301 ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg ttttcgatac ggatgccttc
     9361 cattactttt tcccacaatt ccgtggtggc aggcgtcgct tcggcgagga aagattcatc
     9421 gccttcatac ggtgtatagt tatgttgaat aaaatcgcgg acattaattt cgtttttcca
     9481 gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc ttatcgctgg tatcaatatc
     9541 tatcttcatg aaaaataatc tctctacaat acttcaacta aattatgcaa attctgcggg
     9601 cgcgttaact ttgcctaaat gaatggcatc caaagcaatc attttttctt cgttagtcgg
     9661 aataacggca caaatgacac gcgcattttc actggaaaca attcgctctc caaaagagtt
     9721 agagcgatta ttcatttctg tatcaatcac tacgcctaat acagccaaat gttccatgac
     9781 cagacgacga attaaacttg aattctctcc tattccgccg gtgaatataa ttccatccag
     9841 gcgacgtaat gaagctgcgt gtccggcaat atgacgggca attcggtgaa caaaggtttt
     9901 aattgccagt tgcgcgcgtt cgtgaccttc atgccaggct ttttccagaa cacgtaaatc
     9961 cgaagaaaga ccggaaatac ctaataatcc cgactcttta ttcactacgc gttccaggtc
    10021 acccaggctc tggttggttt ggctggcgac ccaggacatc gcaccaaagt cgacatcgcc
    10081 actgcgggtt cccatcatca agccttccag cggcgtcatt cccattgaag tatcaacact
    10141 ctgaccgttg cgaaccgcgc agattgacgc gccattgcca agatgcgcca caaccaggcc
    10201 ggagtcatct tccgccagat tcagcagaga atgtgcgcgc tgggaaacat agcggtgcga
    10261 cgtgccgtgg aaaccataac ggcgtacacc taactcttca taatatttcc acggcaggcc
    10321 gtataaataa gcttccggag ccatcgtctg gtggaaactg gtatcaaata ccgccacctg
    10381 agttacgccc ggaaataatt gctgcgccga ttcaatacca cttaaattgg cgtaattatg
    10441 caggggtgcc agtggagaaa cgcgacggat attatcaatg acttcatcgg taataatggc
    10501 ggactcggta aaaatactgc cgccgtgagc gatgcggtgg ccaattaagg ccacactgtc
    10561 atttaaattc cgtttttcca gttcaaatgc aattgccttc aatgcacctt cgtagctgtg
    10621 gtgagccagc ggtgctggct ctcccccatt tacggataag aatgcatttt ccgagttaat
    10681 accgtcggca atacctgaca ttaatacttc acagtcgctg gcatcgagca cggaaaactt
    10741 aatcgaagac gaaccacagt taataaccaa aacaaccgga aattcattca tctcttttct
    10801 catcctgagt tacggattaa aacagtttgt atacgatgtt caggatggtc agcagaccaa
    10861 tcacggtaac aaacacgtta tccagacgac cacggtattt cgccagagac ggcgctttac
    10921 ggatggcata catcggcaac aggcacagca gggatgcgat aatcggtgcg cccatggctt
    10981 caatcaggtc gaggatgttc gggttggcgt aggcaacaac ccaggtggag cccatgatga
    11041 agatcatgct gatagtgttc agtttaccca gcgacacttt agttttgtcg cctttataac
    11101 caaacttcag gaccaggcca ttcagacctt ccagcgttcc cagatagtga ccgaagaaag
    11161 atttgaagat agccacgagt gcgatgatgg aagccgcata ttccagtgta atcgcgaacg
    11221 ttgttttggt accggtcatg gacgcaaagt ggttagccag ataagaaagc actggaatat
    11281 tctgcgcttt ggcttccgcc atgttggccg gagacagcgt aaataggcag ctaaaggcaa
    11341 agaacatcac cactgcaacc atcagcatgc tggcacgaga aatgatttgg gaacatttac
    11401 gttcggtgaa gtcgcgaccg aagtctttct catactcttc acgtttagaa accacgaagg
    11461 aagagacgat tggcgagaaa ttaaaggaga aaaccatgat ggaaatcccc agccagacgg
    11521 tgatcaggat accgtcatga ccggttaacg acagcgaacc gaggtcaacc tggtcgataa
    11581 ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag caccaggctg gcgataaacg
    11641 gccataccag gtagctcatc actttaacca tcagatcctt accaaaccag atgacgaaag
    11701 ccatcagcag cagcaggaac agcgccacaa agccgcgatt cagcggtgca aagccgagct
    11761 ggttttccca gaacgtcata aaggtattgg taatagtaac gccataaatc cacagcagtg
    11821 ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc gccagtttta ccaaaatgct
    11881 cttccaccgt ttccgtaatg ttgccggaag ggttagagcc agaaagacat agacgcgcca
    11941 gcgcccggtg gcaataaaat gcgatggggt atgccaatac caacatcaga agaatcggga
    12001 tcagtccgcc aaaacctgcg cggataggga agaacagcac cccggcgccg attgccgtac
    12061 caaacaagcc taacgtccat gtggtatctg atttacgcca ggacgattgt tttgtctggc
    12121 tggatacaat gctatctgaa gtactcatat cctatcctca acgaattaat taagcgtcaa
    12181 cgaaaccggt gatttgagag acgcgagaaa gatcgatatt gccgccggaa ataatactga
    12241 cggtttttct gttttgaata tattggtcta atttaccgct taataatgca gcacatgcca
    12301 gagcgcctgc gccttcggtg acgactttat tgcgctgaat taaggcaatc atactgtttc
    12361 tgatttcgtc ttcgctgacc agcacgatgt catcgactaa ttcacgaacg atttcgtaag
    12421 ttaaattacc cgggcgggag acatcacaac catccgccag ggtgccggta gttcggtgcg
    12481 tggttatttc tccggagtgg aaagaagccg ccatgccgtg aacgttttca gactgtacgc
    12541 caataacacg aatggtcggg ttaatagatt taattgccac cgcaatacca gcaattaaac
    12601 cgccaccacc aattggcaca atcacgttat cgacatcata gagatcttcc ataatttcca
    12661 gaccaatcgt tccctggcca gcaatcactt tcggatcatc gtaaggtggg ataaaaatac
    12721 ggccttccat ttcgacaatt tcgctcactt tagcgatagt gtcgttgaag ttatcaccat
    12781 gcagaacgac ttctgcggag tagtcgcacg ttgccgctac tttggatttt ggcgcacctt
    12841 ttggcatcac cactttaccg tcgataccca gcatcgcgca ggagagcgaa accccttgcg
    12901 catggttgcc cgcagaacag gccaccacgc ctttgcgttt ttccgcatcg gtcagtgaac
    12961 ttaatttatt aaatgcgcca cgaattttaa atgaacccgt acgctgcata ttttcaaact
    13021 tcaggaatat ttcacctttg caacgttcac taaaatagtt ggagcgaggc atgcctgttt
    13081 tataaattcg cccagccagt cgttgtttcg cttcaataat atcatcaata gcaatcggca
    13141 gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa ttaaatatgt aggtaaccgt
    13201 aaccgacacc tgcaaaacag acaggtggat tatttatgtt taaataaaaa caaataacta
    13261 accaacttct attaattgcc ttcgtctaca cccattataa gatgaatact ctttggctaa
    13321 ttccaccaaa accgatgctg cttttttaat acgataattt ttcgaccata ccgcggcata
    13381 ttgtgccaca ggtaatgttt cttcaaccgg aatagtaata aattgattag aaccaaaagg
    13441 tgacgtcata tcacaaggaa ttacagttaa gaaatcagca ttgagaacaa gattataaat
    13501 tgtcacgact gagtcggttt taacgatgtt ttcaatactg atgccatttc tttgtaacgt
    13561 agtaagcagt tcgctgtagt accccatatt agtttgtggc aacacccact gttcgttctt
    13621 caacgactcc agcgtggtgg tgccggtgca tgttcgggac ttactggcta ccagcacaaa
    13681 ctcggactcg aacagcggct caacatgtaa atcctgaagc ttcatttctg cacttaatgt
    13741 accaatcgca aaatccagtc gaccgtcgcg gattgccggt aagaacgaag acagttgcgc
    13801 ttcatacata gaaacctgcg ctttcgggaa cacctctttg aacttgttga tcatccctga
    13861 cataaaagta aaaccaatca atgaaggaaa accaaatgag acttccacca ccgcctcaga
    13921 agacataccg cttatctcat taaccatatt tttcatttca cgggtaatgg attcggaacg
    13981 ggagagtaac aattgaccgg caggtgttaa tgttacaccg gtatttttcc gcaccactaa
    14041 ttccacacca aaataatctt caatatcgtt aatgatttta ctgacggccg gttgagttaa
    14101 ccctaattct tttgcagccg agccgataga gccacttcta atgacttcct gaaagactac
    14161 caggtgctgc gttttcggaa gaagaatagt gctcataacg acctacgtta attacctcat
    14221 tgacggcatg aagtgtatca aaatgaaatg aacaggatat gtgcgaccac tcacaaatta
    14281 actttcaata ctttccagg
//
LOCUS       DAZOXR010000104        14107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZOXR010000104 DAZOXR010000000
VERSION     DAZOXR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.106"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(13..1371)
                     /gene="hprS"
                     /locus_tag="ACHUNN_002703"
     CDS             complement(13..1371)
                     /gene="hprS"
                     /locus_tag="ACHUNN_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0577042.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="ACHUNN_002704"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="ACHUNN_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0577043.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="ACHUNN_002705"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="ACHUNN_002705"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0577044.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="ACHUNN_002706"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="ACHUNN_002706"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR0577045.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="ACHUNN_002707"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="ACHUNN_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0577046.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="ACHUNN_002708"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="ACHUNN_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0577047.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="ACHUNN_002709"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="ACHUNN_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="ACHUNN_002710"
     tRNA            complement(6687..6776)
                     /locus_tag="ACHUNN_002710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="ACHUNN_002711"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="ACHUNN_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0577048.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="ACHUNN_002712"
     tRNA            7768..7843
                     /locus_tag="ACHUNN_002712"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="ACHUNN_002713"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="ACHUNN_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3990 a         3353 c         3171 g         3593 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta atagcagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DAZOXR010000105        14012 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZOXR010000105 DAZOXR010000000
VERSION     DAZOXR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.107"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..592)
                     /locus_tag="ACHUNN_002714"
                     /pseudo
     CDS             complement(1..592)
                     /locus_tag="ACHUNN_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
     gene            complement(749..2929)
                     /locus_tag="ACHUNN_002715"
     CDS             complement(749..2929)
                     /locus_tag="ACHUNN_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0577049.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(3028..3234)
                     /locus_tag="ACHUNN_002716"
     CDS             complement(3028..3234)
                     /locus_tag="ACHUNN_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577050.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            complement(3259..3969)
                     /locus_tag="ACHUNN_002717"
     CDS             complement(3259..3969)
                     /locus_tag="ACHUNN_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0577051.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            complement(4445..4750)
                     /locus_tag="ACHUNN_002718"
     CDS             complement(4445..4750)
                     /locus_tag="ACHUNN_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577052.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            complement(4897..5112)
                     /locus_tag="ACHUNN_002719"
     CDS             complement(4897..5112)
                     /locus_tag="ACHUNN_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577053.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            complement(5333..5623)
                     /locus_tag="ACHUNN_002720"
     CDS             complement(5333..5623)
                     /locus_tag="ACHUNN_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577054.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            complement(5947..6243)
                     /locus_tag="ACHUNN_002721"
     CDS             complement(5947..6243)
                     /locus_tag="ACHUNN_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577055.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            complement(6243..7196)
                     /locus_tag="ACHUNN_002722"
     CDS             complement(6243..7196)
                     /locus_tag="ACHUNN_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0577056.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            complement(7579..8241)
                     /locus_tag="ACHUNN_002723"
     CDS             complement(7579..8241)
                     /locus_tag="ACHUNN_002723"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR0577057.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            8757..9326
                     /locus_tag="ACHUNN_002724"
     CDS             8757..9326
                     /locus_tag="ACHUNN_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0577058.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            10054..10227
                     /locus_tag="ACHUNN_002725"
     CDS             10054..10227
                     /locus_tag="ACHUNN_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0577059.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(10485..11813)
                     /gene="rutG"
                     /locus_tag="ACHUNN_002726"
     CDS             complement(10485..11813)
                     /gene="rutG"
                     /locus_tag="ACHUNN_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0577060.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(11834..12328)
                     /gene="rutF"
                     /locus_tag="ACHUNN_002727"
     CDS             complement(11834..12328)
                     /gene="rutF"
                     /locus_tag="ACHUNN_002727"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0577061.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(12339..12929)
                     /locus_tag="ACHUNN_002728"
     CDS             complement(12339..12929)
                     /locus_tag="ACHUNN_002728"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0577062.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(12939..13739)
                     /gene="rutD"
                     /locus_tag="ACHUNN_002729"
     CDS             complement(12939..13739)
                     /gene="rutD"
                     /locus_tag="ACHUNN_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0577063.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(13747..>14012)
                     /gene="rutC"
                     /locus_tag="ACHUNN_002730"
     CDS             complement(13747..>14012)
                     /gene="rutC"
                     /locus_tag="ACHUNN_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0577064.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         3844 a         3255 c         2896 g         4017 t
ORIGIN      
        1 ccaagcctta ttacatttaa tctgagcatc gagaatatca tttctgatag ctggagcgta
       61 accggataca tcgctaatat tcattaactc tttgcacgat agcataggcg cgttattgcg
      121 ttcagtggtc acgttacctg cattcttctt actggtcaca ccaccgtcat ttgtggtagc
      181 cgttttgcca gtggctgctg aaatatcagt ttgacctacg attgctttaa agttaatttc
      241 ctgaaaagtt agctggatac gaagaccgtt agagatagta acatctttag aggctttaaa
      301 gttggtaata atggaatcgt caattttaat tccatcttta cagataacag aaataatctg
      361 cttctggtca cgccagtttt ccagagtgtc aatgaagttt tctactaatt gaccctgacg
      421 agttaataat aagcttcctt cataattgca tgttctgtgt agtcacctgc atagggctat
      481 caaattccat gttttcagtt gctgataagg ttaagaaggc atctacattg tctcttaagt
      541 ggaaatagat gccatctttg ccactgtaac gaagctgatt agtgtaaatc attaaattct
      601 cctttgattt caaaggctta gtgtatttta atcattttta accaagcctt tgatatatat
      661 gtattttctc tgttaagcgt gagctacgtg aaaaataatt tttggtgtgg gtattaactt
      721 tgctttattc acgtagaaca ccgttaaatc attcttcctc tggatagctt gaacttaaga
      781 gcatatcctg cgtgaactgg tcattagctg attgattgaa agtaaagaaa gatggttcat
      841 ctggtcgaac gacaacttga ccaccaagtg aaactttcac tggctcctga atacgcttac
      901 tcaaagcact aatttcatcc ctcagaccct gaatatctgc actcgttaat gctgaatctt
      961 gcttaatgtt gtttaactct ggatgattga atcttgcaat attcattgtg gcattgttaa
     1021 acaattcacc cattgaacta aaaattctac tccatccttc gttaatcata ttcggtttat
     1081 tagggtcacc agaatacacg ggattaaagt cactacccac acctacccca gacggatgca
     1141 tattagcgtt tttgactact tcctgattca taaaatccag tgctttcttc aatgcatata
     1201 aaccaagagt gacgcctctg atttgtggaa tcattgaagc taatgctaac gctgtttcat
     1261 aaccgtcatc agcataacca attgcaagag cactacctac accaccagct acacctttca
     1321 gttttccacc acctttacca gtagctacct gaccagccac ctgattagct cttgtaccta
     1381 acgttgtgat tgctcttgtc aggttcataa cccctgtagt agcactgaat agcgtctttg
     1441 cagataacgc tatagcaaga cctttgacga agttgataaa accatcaccg acttcatcaa
     1501 gtagttttcg tttagtgtca tcaaggctgt aatagaactt cttagtctgt aaaatccagc
     1561 cttcaatggt gttattgaat acgcgaatat catctacacc agctttaagc atgtcgataa
     1621 gtccaccgat agcatcacca gcgatagaag aactcatccc catttcacta agcaggttac
     1681 tcaggctgga taagaagccg tttagctcat cctgtgaacc tgagaagatt ttcaccatcg
     1741 tatcatccca attgtttgct aacttaccta aagctgtctg agtgctctta gtgacttcag
     1801 cccagccacc cgctttatca atcatcaagc ccatctgttt agcgacttta gggagagtat
     1861 cagcggaaag taccttcccg tttttcatgg ctgcatcaag ctctgtagca gtcataccca
     1921 atgcttcagc gaatagctta aatgcaccag ccatacggtc acctaactgc ccacgtaatt
     1981 cctcagcctg aatcttgccc ttgctcgcca tctgctcaaa tgctctcaaa atgccgttta
     2041 gctcatcgtt agaaagcccc attaagcgac tcatagccgt tgcattacca aatagctcct
     2101 gtgtctgttc ctgtgacata gaaccctgag cacctgctgc aaacttagcg tagcttgata
     2161 gcccttcaga gatattaaga cctgtcttgt ctgctaaatt tcgaataaat acctcagctt
     2221 cagatgcttt atcaccaagt acaaacttag cggctctctg tgcggaatct cgtttaagac
     2281 ctgcattcat aatggccttg taagcgttaa gagcacccat cacagagtaa aatgcaccag
     2341 ataatttaat cgcattgctt gcaagattat taaatgcgtc gcttgtattg ttaatgctgg
     2401 cggtctttgt ctgtgacctg agtttattca acgtttcacg aagagattga tttaattttg
     2461 caagttcagc gttaaacttt tcacttccaa tattaccagc cttaccgctt aagctgtaat
     2521 actgtgcttt aaacatttta gccatcagcg gattaatatc accaagctta gacatattca
     2581 cgctatgcgc tgctgtgatt gctctgtttc cgctaattcg tggttgcctt gattgttttg
     2641 gaggattaac agtgtcatta aatctggtgg caaactgaaa tgcttttgtg cttcttgctc
     2701 tctgctcaat cttgtcaaag gtcttattaa acttcctttc aagctgccct aaagaacttg
     2761 tcagattgct gtcaagttgc ttggtatcga ccttaaacgc tctattcaaa ttgcgttcaa
     2821 ttctttgtgc tgactgcaaa gctgcctttt caactctttg caagccctta ataacgtttt
     2881 cactgaaact aagttcaacg ataaaactat caactgtgaa tttgctcatt catgtttcac
     2941 ctctttaagt gttcatatac gaaaaaggga aagactaccg atatcactcg ataatctctc
     3001 ccgtagattt tcacccaacc aatcttgtta cctaaccaga taataattac catcttcacc
     3061 aacaatctga caatcacttg gtaatttatc aagacaccac ttagctattt tcctgattct
     3121 attcttttgc ttagaggagg tgaatgattt gattcgtttt ggtttgttgg gttgttcttt
     3181 caatcgttca ctaattgctt taataagctc atagcgttct gttaaatcaa tcattcacca
     3241 ccacctttta attattaatt aaatagcctt tgcatatttg ctagaaatct tcgtaagctg
     3301 ctcaatatca gcctgcatag caatcttctt agcatcttca tattctttca caagctcttt
     3361 catagcctga agctctcttg agagatacaa cttaacatta tcaagttgct tgttacgtga
     3421 tgccaagatg ttctgtctag caatttcagc gagttcttca tcgctcttat tagtcatgta
     3481 aataaactga ccagcacctc ttgatgcctt aaccatcttc acaattcgat gtccagcagg
     3541 aagactacta atcacggctg cgacgttttc ttgaaaagta ccaaaaccat cattatcttc
     3601 gtattggtaa tagacaaatg gtttctgtga caattcatcc tgaatctcaa attctgacgg
     3661 ctcaatagct gattgcatca aggtaacttt gctgtaagcg tcatctaatc taaggcgctg
     3721 ttctttcagc agttggtaat atttcttttc aagctcaagt aatgcttcac tcttgcaaat
     3781 atcaggcatc tcttgaggct caggagacac aaagatggtt gtttttggtg acatctttag
     3841 cgattgctca agctcaatac gcttgtcatg ctctttacgc tgaatagtga tagctggatg
     3901 ttcagtctgt ggcgaccagt cactgaagct atcaatcact ttgccattaa tgtaaatatc
     3961 atttgtcatt gcttttcctc ttctaagcgg aagtgtttca gtttttaaaa agcacacctg
     4021 acagcctatc taaacttaga agagacttaa aaggcggttt gccaagtatg ctttttaaaa
     4081 gctgaatagc cctagataat ttcttataaa gggccattca taaggtgaag aaattaaaac
     4141 atctaaagga ttcaaaaagg attcaaacga atgaacaaga gtacttttcg agatgacctc
     4201 tcagactaac ctgaaaggtc atgtagcgaa aagcattgca cctttcagaa taactatcat
     4261 tgatgtagaa tgattaatag tttaattcag gaagagttgc tcttccctat taccatcatc
     4321 tcatgcttaa ggttatttgt caacagcttt ttaacagatt ggtaacatca attcttattt
     4381 accgtattac ctacccagca accaacaaat aaccctaaag tcatccagag aataacgagt
     4441 gtcattacat cacctcgctg taagggttta attcattctg ataggtcaag aatacattca
     4501 gcttcaataa ttgctcaggg cttaaatcaa gtcgattgca ctcttcaatg ataaagcgct
     4561 tattgtatgg gtctgttact tccagcttgt gaacatagtc accaagttta atttcgttga
     4621 ttatatgctt gtcaatatac gttgacgctt caaaacaatg catgaaatag atattaaagc
     4681 ccgacctgtt caggtcaatc aaccaattca tggtttcttg gtcaaccata atttgcattg
     4741 ggtaattcat ttttaaaatc cttcctgtat cgcattctaa gacgttttaa cccctttttt
     4801 agaccactta aggcgattcc ctaagttacc ctgattaggg gcttaaaaac gtttctggtg
     4861 cgtctggtaa agcctgagag atatgttttg ctgtttttag ctcaagtctg agactaatag
     4921 ccccatctgg tgaccgtagt cttttaacca gctttgctta accggagtct tcagagaata
     4981 gttaggggct gacaagtgaa gatagatatc atctttgccg tggatacttc cccatccttc
     5041 gtataactca agcaagtttt caaaataacg aaatgcttta ttagtctgaa gttgtactgt
     5101 gatagttttc atcaagcacc ccattcagaa ataagcttaa ttccctgctc aagctgttca
     5161 gggtcgttat agtcgagtat taccagtggc tttaattcgt gtttaagctg gtggatttga
     5221 gagggtttta atcgtttgtt tgctttgagg gtcacatctt caaattcagc atctgctaaa
     5281 ttctgtctga ttaggtggcg gccaagtgtg gaatattgga acctataaca cattaaacct
     5341 ccaatctatc cgccagttga tggaccttag cgcttaacac ctgaatcatt gagcaacctg
     5401 ctactgcgga ctgtgctttg agtagttcca gttggcccgt tgtgattctg atggccttag
     5461 aaccctctgc gacgccctta acgttatcga aagggctaag gtttaccgac tcttttaaaa
     5521 tctccccaac aagcgctgtg tgcttcatat cgggatattt ggctttgatt gctgccagtc
     5581 gttcaaatgc ctctggcaaa aggcgcgtaa ctgtgtgagt cattgtaaaa atctccttaa
     5641 atctcttact catactacaa tcttaacatc tatttaacat attgtcagta cctcatttac
     5701 atgatactat ggttgtaggg gctgatacag gtgctgtttc ctttcacaaa aggaaaatta
     5761 atgcatgatt ttttcacagc aattattgaa tctcatcgtt ttagctcgtt gggctttgcg
     5821 gtatttattg tgctattggt gttttgcgtt atgaatcgag tcattacaaa aaagacaaag
     5881 cttacaattc gacttaaact tcttcgtgtt gagatagaga tttcagtaaa taaatagctg
     5941 gtggccttac cggcactttc ccttgataat attggtggcc ctcagattac cggggtcacc
     6001 gtcttttaat cgtagctggt ggactgaatc ccataaatcg taatgataca gcaccgcaaa
     6061 cttcaaagcg tcaatcttta aatctgtcac atcatcgacg aaataataat ccctcccttc
     6121 tttcttcggt cgaatccggt aaacttcccc gtgagtatct ttcatcaagt aaccttcacg
     6181 agaaatgctg taacgtctga gtaaatagtc acggtatttt ctctcgccat tataagtttt
     6241 cattaaaaca ttcctccacc ataaacacgt ctgactttaa ctttttcttt ctttggctcg
     6301 gtgtgagttt tcttttttgg tgtaaaagta aaatcatcgt cactatcatc aaagtcatcg
     6361 aggtcactaa ccttgttctt tagcttcgca tttctgaatc tttctttaat ttctgcttta
     6421 cgctgttcac gtttcaattc ctcttcaggg tcttcttcat catcaaggtc aacatccggt
     6481 aattcatcat cagtggcggg gcactgtgtg tttttatggt cactactagc aatcacatca
     6541 gagattttat cttccttttt aacgacctct ttagcagctt cactatcaag gtcatctaag
     6601 tcagatgttt taacagtctc tttaatagcc tcttcaatac caagagaatc acattcatgt
     6661 gaatcacaga gcgaagctcc cttcttaaaa tattcttctt caaagtcatc ttctttattg
     6721 ttattaataa gttctttctt gttattactg ttattatacg tgtcaaagat tttgactgac
     6781 tgagtaaaaa attttgactc agtgggtaaa gaattttgac tcagtgggtc atctttcttg
     6841 actgactgag taactttaga ctccagcttt gcctttctgg cttccttagc tgtaagctct
     6901 gttaaaacct ccggtacttc atcaagtgaa taaactttaa taacacactg attgcttgca
     6961 acgttagttt tcttgattaa accatgtgcc tcaagttgtt tcatacatgc aatcacggat
     7021 gttcttgtga ttccagcata ctgaccaaga tgagcatatg aaggggtata atattgcccc
     7081 ttatcgtgat aaaacttttg ttcattcaac atgacagtgt aaataatacg gtcggcagct
     7141 ttaacgccat tagtcatctg ataaatacgg tcatcaaaca tgtggaattt ttccattata
     7201 ttttcctttt taaaaataaa acaaagaaag ggctgaccac gtatagtgac caaccccaga
     7261 gggagtgaat taattagaag atggactgat aaaggaactc agaagcctga gcgattacgc
     7321 gagtattaac agcaaaagga aaagccttga ttgaataaga ggccgtgaaa atctgtcaat
     7381 accctgacgc ctcccaacgt tgtaacctct taatgttctg agttctttta tcaccccata
     7441 taaaaactat aacagatgtg attataaatt gcaacaatta tttcacagaa taattactga
     7501 ttggtcattt attgttcaca gggatacttc ttaatatctg aatcacctat cttgtaaatg
     7561 accatattta tgagaatttt agctcaacct ttgagcaatc ctgtatgacg tgctcagagg
     7621 cattctgaga gcatctgcaa tgcacttaat gggttttcct tgcctgtata gttttatgaa
     7681 gctgtcgagc gtctcaggtg ggtaaatggt gggtcttcct ctcaagcctc ctctgttaat
     7741 gatggttaac ggtaatagcg tgtcaccaca gcaaaccgtc tgtgtaattt cgtgcttacg
     7801 tggttctttt ttaaaaacaa cgactgcact aggaaacggt gaacgaatag taagcccgtt
     7861 atggcctgct ggtggaaggt atttaactgc acgatgaaga atacggattt catgacagtg
     7921 gttattgctg taaatgtgcc taagccagta gctggtgttg gctctatgcg gtatcaggaa
     7981 agctactaaa tccgcttcag acgcttttaa gaggaacgac ggaatatcgc tgtacggcag
     8041 gttacagaag accttttcac taacccatga ttgacggctt atatcattgg tgaatcgtgg
     8101 taacagcgtg tcatgctcag ttgcagcacc atctagcgta aaaccaaatt cacggtgtaa
     8161 gttcttaaat aacaacaatg gagttctcca gtaatcagaa cccccatgat gtttcccgat
     8221 taaaatatgc tcaattgaca taagaaataa aaaattttat tgcaatcact cttgacaagg
     8281 tttactctct tttaagataa gagatactta acaggctttc taaacggata tcctttctgg
     8341 aatatctatc tcttatctta aaagtcttta cagatacttt aaagatatct gtattaaatc
     8401 tgtacagtaa cttttcagaa tctgaaaatt tttataaaat cgtagtagcc cctcttcaaa
     8461 ttccccttct aaaaatcttc gaaaatatct gacagatgtc tgtagctgct aagtacccgt
     8521 cacaccagct atgtgacccc ctttgcaaaa aagccaaata tcattgactt tctcggtgaa
     8581 ttgctataaa ggcatctatc taggtcttta actgtataaa tacacatgac aacaaacgcc
     8641 cgttaagtgt ctatataaaa aggtagctgt agctatagac aacaattgtg ataattgatt
     8701 taatggctat agacaagcaa gacagacctt tgttgactat atagagagat aagaacatgc
     8761 gtacattcct ttatgctcgt gtatcagttg atgacctcac cacacagaac caagtagaag
     8821 ctgttaaacg tgctggttat gaagtcagac cttcacgagt aattgaggaa gttattagtg
     8881 gggctacacc agctatggac agaccacaat ttgtaaagct ggtggataag ctggaagaag
     8941 gtgattgtct tgtagtgacc aagattgacc gcttaggaag ggataacatt gacgtacaga
     9001 agaccgtcac aatgttactt ggtatgggtg ttaagctggt ctgtctagat ttgcctgtcc
     9061 aagacctttc aagtgctgaa ggtaaactta ttcttcaact attcagtacc tttgcagagt
     9121 ttgaacttaa ccgcatcaga gagcgaacca gagagggctt agaacgagct aaagcacaag
     9181 gtaaggtgtt gggtcgtcct gtagctaaaa acactacaga ggccgtttta gagcataagg
     9241 ctaagggatt gtcacaaagt caggttgcaa agcttctgaa tctctctgtg agaacggtta
     9301 gccgtcactg gacaaagaca ctgtaaaaaa aacttagtag catcttcata gcctccttca
     9361 tgggggcttt tttgtgtctg taacatatgg tgaccaaaca tctatatatc gttatatagc
     9421 cctgtatata gcactatata tagtgtctgg agagggacga ctaacaccct gcatagttcc
     9481 ctgaaaaggg gcttcacaga ttctctatat atttcaataa gttaggataa aaagggacta
     9541 taccgatact gcatagaatc tgtgtaggat tgcatagtca taggggttgg aacaagccca
     9601 actacacctg ataaagcctg ttattaacag gtagttgaat gtacgtgttt agctatgtaa
     9661 aagcaccaga actttagcca tttctaacca ctcctcgtgt tatctctatt ccgtagcgat
     9721 tcgctaccac ttatttaaag ataagacgtc cttttcagag tgcaaatttc acaaccactt
     9781 atttgattta taacaacttt cacaagcacg taattttgtc gcaaaatgac acatttttat
     9841 ctcatcgcgt ttttttaatc ataagagcgg cttatggata attattggag atgatatcta
     9901 ttctcgctaa gaagctgttg caggatatta ccaaacgcgg gtctacccgc gtcagttcac
     9961 taagcttagt cccacgtagc gaaaatatgg cagccgccat acgccgcgtt aattctatgc
    10021 aatatgatgt ctatacccag acggaggtca gtaatggcaa accatcgagg cggttccggc
    10081 aattttgcag aagaccgcga aagagcatca gaagcaggta aaaaaggtgg acagcacagc
    10141 gggggtaatt tcaagaatga cccgcagcgc gcatctgaag caggtaaaaa aggtggcaag
    10201 agcagtcacg gcaaaagcga caactagccg ggctaatcaa tgacgaatgc atttttgtct
    10261 gtagctcgtc aaaaagccat caccgccggt tacccggtgg ttgatactga tgacaaatgt
    10321 aagcttgcct gatgcgcgat gcttatcagg cctaccagaa gattgcaata tattgaattt
    10381 gcactgtttt gtaggccgga taaggcgttt acgccgcatc cggcatgaac aatgcgtacg
    10441 ttgtcaacaa tctgcaccgc cgggtaaccc cggcggtttt ctgtttatgg ctcctgatga
    10501 acaacttctg gcggtggaac gtcaaccaac cttctactta acaacgcatt gagtaaaatc
    10561 gcgccaaagg tggctgtacc gatccctccc aacgtaaaac cgcccagcgt gagagcaaaa
    10621 tcacccgcgc ccagcactaa ggttactgcg accataatca aattaccgtt ctggcttaaa
    10681 tcgacacggt tttgtaccca gatccttgcg cctgcgacgg caatcagccc gaacacaaca
    10741 attgatgcac caccaatcac cgcggccgga atggtatgga tcagcgcgcc aaatttcggc
    10801 gaaaagccca acagcatggc gatgacggca gcagcaacaa acaccagcgt cgagtagact
    10861 tttgtcacgg ccatcacacc gatattttca gcataggtgg tgacgccgct accgccgaca
    10921 gagccggaaa gcatcgttgc cagaccatca ccgacgaatg ctcgccccat atacgggtcc
    10981 atattgcgtc cggtcatccc ggcgaccgcc ttgagatgac ctaagttttc cgccaccaga
    11041 atcaccgcca cgggcgcaat cagcatcatt gcctgactat taaaagcagg agtggaaaaa
    11101 tgtggcaaac cgaaccaggc agcatggctg acgagagtaa aatcgacggc ttttcccagc
    11161 cctaaaacgt tggtcatcac gccatacagc agacaggcga caattaaacc tacgagaatc
    11221 aataaccgct ggatcatgcc acgggtaaac accgccacca gcccaataca cagcaccgtc
    11281 attaccgcca tccagctatc aaaggctgaa gccgatacac ttttcactgc gataggcgct
    11341 aagttcaggc caatcgccat caccaccgca cccgtcacca ccggcggcat tagtcgttca
    11401 atccagcgtg taccaatttt catcaccacc aggccaatga cggtataaac cagcccacag
    11461 gcgataatcc cgcccagtgc gatgctgata ttcgggttaa tgccctgacc gttaaagccc
    11521 gtcgcggcaa tcaccacgcc gacaaaagcc gcgctggagc cgagataact ggggacgcgc
    11581 ccgccggtaa taaagaaaaa cagtaacgtg ccgatccccg acattaaaat ggaaagattg
    11641 ggatccagcc ccatcagaat cggcattaac accgtcgcgc caaacatcgc caccgcgtgt
    11701 tgaacgccca ttactgccgt ctgagcaaac ggcaatcgtt catccggcgc gaccacgccg
    11761 ctctctgtag aggtcgattt taactgccag tgaggaaaac cgaacattgc catcagctgt
    11821 ctccttaagg aggttaacaa gcagggcgca tcagcgcgtg ataactgcga tcgaaccaca
    11881 ccagcccgta gggtgtggtg tgacgatgaa tcgcttcgat ggcgcaaaac agaatgtcgt
    11941 gggtgccgac gctcaccacc tggctgatgc ggcagtcaaa cgaaaccaga gcctcttcca
    12001 gttgcgggca tccggtcaca cccgtctgcc agcgggcggc ggcaaagcgg tgttccatgg
    12061 gcgttttgcc gccaaaaaga tttgaaagcg gctcctgccc ggcgctcagt gtatttacac
    12121 acagcgttcg attttcattg aataccggcc agacggacgc cccacgattc aggcacacca
    12181 gtaatgtggg cggcgtgtcg gtcacgctgc agacggcgct ggcggtgaac ccggcgcgcc
    12241 cggatggacc gtccgtggtg atgatattga ccgccgcgcc catacaggac atcgcatcgc
    12301 gaaaggtttg ttgatcaact atgttcatag tttgctcctt acaacagccc gcaggcttct
    12361 tcaaaggaca gacgtggcag gcgcgcaaaa agtttgctgc tatcgccata gccgatatta
    12421 atcagcagat tgctcttcag cgtgctgccc gcaaaaaagg cgtcgtcaac gtgttgacgg
    12481 tcaaaacccg acatcgggcc ggtatccagt cccagcgcac ggcaagcgac gatcagatag
    12541 gccgcctgca tggagctgtt gcgaaacgct gtttcttcgg caagttgtgg gctggaggta
    12601 aaccaactgc gggcatcacc gtggggaaac agtagtggta accgttcata aaattcactg
    12661 tcccaggcaa cgatagcggt gacgggcgcg gtcagggttt tttgtagatt gccgctggaa
    12721 agcgccgggt gcagacgttc ttttccttct acagtgcgga taaacacgat ccgtgccgga
    12781 gaacagttag ctgatgtcgg cccccatttt atcggggcat aaagctcccg taacgtctca
    12841 tcgctgacgg gtgtctcccg ccagccgttg tgagtgcggg catcggtgaa cagggtgcta
    12901 agcgcacctg ggctaacggc ttcgttcata gcaattcctt acagggcggc ttcacggtga
    12961 tgtaacaggc tggcaagccc gttgagtaac agagcattaa acgtttcggg gtcggtcacg
    13021 ttgcaggcgt gtccgccata gcgcatcacc attttctggc tatcgggcag ggcggcatga
    13081 agctcactgg aacatgctga tggcaccagc agatcatcac tggcgcagat gatttgcacc
    13141 gggcagcgga tgcgatccgc atggtgacta aagtcagcgc gtttgagggc gttaagtcga
    13201 cgcagtaaat tatttttgcc ctgaaaatgc gccagtgcca gcgcgtcttc tgcctcaagg
    13261 cgaggtgcgc gggccgccat ccagtcggcg ggatagagga acaacggctg cgcttccacc
    13321 catgcctgcg cgccgccgct atacagtaat cgttcgcgaa cctgaaaaca gcggcgcgta
    13381 tgggcgttta ttcgtagcca gccgttaacg ctgatcagca cagttaccga cgcgggataa
    13441 tccagcgcca gctgcattcc caccagcgca ccgagcgcat ggccgaccac cgcgtaatgc
    13501 tcaatccctg cggctaccag cgcctgatgc agttccgctg ccatctgggc gatactgtaa
    13561 tcttctgcca gcgtgtcggg attattgccg gtgccgcgct ggtcgtaaca gactacctga
    13621 tactcctgct ccagcaccgc cagttgcggt aaccagtaac tgccgctacc gccaagaccc
    13681 gaaatcaaca ccactacggg cgcatcagca taagggggag gtgagagtga aagtttcatc
    13741 gcggcctcac ttggcgatat gcgcaattgt ggcgatttcc accagcgcgt caggttttac
    13801 cagtccgcac tgaatgcaga atcgcgccgg tttatcaccc ggaaaaaact cggcgtagat
    13861 ttcgttaatc gcggcgtaat ttttccagtc ggtaataaag atgctgttga aggtcacatc
    13921 cgccatcgtg ccacccgccg tctcgatcac cttgcggata gtttccagaa cgtggcgggt
    13981 ttgcgccttt gggtcatcgg caaacagcac gt
//
LOCUS       DAZOXR010000106        13903 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZOXR010000106 DAZOXR010000000
VERSION     DAZOXR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.108"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="ACHUNN_002731"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="ACHUNN_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0577065.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="ACHUNN_002732"
     CDS             complement(1550..1723)
                     /locus_tag="ACHUNN_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0577066.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="ACHUNN_002733"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="ACHUNN_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0577067.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="ACHUNN_002734"
     CDS             3361..3690
                     /locus_tag="ACHUNN_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0577068.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="ACHUNN_002735"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="ACHUNN_002735"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0577069.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="ACHUNN_002736"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="ACHUNN_002736"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0577070.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="ACHUNN_002737"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="ACHUNN_002737"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0577071.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="ACHUNN_002738"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="ACHUNN_002738"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0577072.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="ACHUNN_002739"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="ACHUNN_002739"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0577073.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="ACHUNN_002740"
     CDS             9613..9909
                     /locus_tag="ACHUNN_002740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577074.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="ACHUNN_002741"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="ACHUNN_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0577075.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="ACHUNN_002742"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="ACHUNN_002742"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0577076.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3437 c         3230 g         3578 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAZOXR010000107        13868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZOXR010000107 DAZOXR010000000
VERSION     DAZOXR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.109"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            76..399
                     /locus_tag="ACHUNN_002743"
     CDS             76..399
                     /locus_tag="ACHUNN_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577077.1"
                     /translation="MANDVGGVTHDQVERIAKIVAREVVGKLGKEIREEIVHEVHEQL
                     KNYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIISKVTSFIITALILGLAAYGV
                     KNGLQ"
     gene            413..1105
                     /locus_tag="ACHUNN_002744"
     CDS             413..1105
                     /locus_tag="ACHUNN_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577078.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDTYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMA"
     gene            1107..1358
                     /locus_tag="ACHUNN_002745"
     CDS             1107..1358
                     /locus_tag="ACHUNN_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577079.1"
                     /translation="MLGSLMIKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVDVKNETLDELRRKDASTVHHELHNKWLRD"
     gene            1684..2082
                     /locus_tag="ACHUNN_002746"
     CDS             1684..2082
                     /locus_tag="ACHUNN_002746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577080.1"
                     /translation="MHTALNASRSIQTIRDCTKMIASVPLRHWNDSLQIREMNKPGML
                     KDQRFIVNLKSDVEEGKFTIKLFAEMMVLNNRLRFLVKPEKQFPDVVRCEQDAFYEIE
                     SKIAKFIKDKYRVDVKIAKLDSSTDMRLAN"
     gene            2083..2859
                     /locus_tag="ACHUNN_002747"
     CDS             2083..2859
                     /locus_tag="ACHUNN_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577081.1"
                     /translation="MSPSFIAEYSKVLSDFPECEAREKIRVGYMVNSDFSVLRLTEWS
                     LIGSNYDYCLTVSFVGDPDADWLQEHAMDLDEQGHSEEEIVHSVRELVGDEQEDDDGI
                     QIARFVYKRFQFRSQKGSHLGVQIKGAFVTPSKEQKGLARRVYNFLLNWHDHVVCDDH
                     QTVYGARIWAVGMLLVGRVQIYDNLNKEFIDVLVEGGIGQNGVKPWDALLLNSEAQLR
                     HWNPMAVSIDPSSQILTIISKEDRHIEVGVTTFDARSTNH"
     gene            3022..3690
                     /locus_tag="ACHUNN_002748"
     CDS             3022..3690
                     /locus_tag="ACHUNN_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0577082.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            3696..4049
                     /locus_tag="ACHUNN_002749"
     CDS             3696..4049
                     /locus_tag="ACHUNN_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577083.1"
                     /translation="MKMGDLAKRKESDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(4110..5168)
                     /locus_tag="ACHUNN_002750"
     CDS             complement(4110..5168)
                     /locus_tag="ACHUNN_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577084.1"
                     /translation="MRMNRLPGYGLPELAFWPQPKYERNKWSMFCLKLRNDGTLAWYR
                     RYVDRGMPNHAVDDDYDNYPEARKAALELNKNVSFDIEKLPLSMFQKKSLRLKVDKAL
                     KAKSRLMDEEYIMLNEAIKAHANDPRVTLNELIINPDYETLRQPLFDVLSEMPYLHFV
                     FLPKFQVSLQLVAPNTWECSYNNKAKTAKICYQERIARGFGLSGAAHWGKTKATIRSM
                     LLPRANRLLQLASVKRMLDEALRNGQKVLVVGNFVFWFEDKNQVGWSVKVASESETTS
                     RGNTLWKEGTIISKNHGRIVVLPYTKENGKHVKGYTKNAPNDGNAIPRHKDEYVELPF
                     EILDGDLMFSLFGELNYE"
     gene            5458..6198
                     /locus_tag="ACHUNN_002751"
     CDS             5458..6198
                     /locus_tag="ACHUNN_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577085.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELVEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILECLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            6242..7582
                     /locus_tag="ACHUNN_002752"
     CDS             6242..7582
                     /locus_tag="ACHUNN_002752"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0577086.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            7730..8845
                     /locus_tag="ACHUNN_002753"
     CDS             7730..8845
                     /locus_tag="ACHUNN_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0577087.1"
                     /translation="MSELKELIAELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGGATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            8921..9697
                     /locus_tag="ACHUNN_002754"
     CDS             8921..9697
                     /locus_tag="ACHUNN_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577088.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRSALVVFISKENPLSLKVQVGAWLDGRVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAWKVVRIA
                     NELPMIEQEAKSYATNHVTELLAPYRLESNQRKMISWGSW"
     gene            9753..10235
                     /locus_tag="ACHUNN_002755"
     CDS             9753..10235
                     /locus_tag="ACHUNN_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577089.1"
                     /translation="MAVVVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTLKRKRDSVTINSMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEVKQQ
                     "
     gene            10232..11554
                     /locus_tag="ACHUNN_002756"
     CDS             10232..11554
                     /locus_tag="ACHUNN_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFR0577090.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESIPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            11551..11766
                     /locus_tag="ACHUNN_002757"
     CDS             11551..11766
                     /locus_tag="ACHUNN_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577091.1"
                     /translation="MIVLSKREKETLHEISKWPEFPQYWKPKTRANLERLGLVANISE
                     TWCSANYQLTDKGKVLLQQLVESGVLK"
     gene            11912..12202
                     /locus_tag="ACHUNN_002758"
     CDS             11912..12202
                     /locus_tag="ACHUNN_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFR0577092.1"
                     /translation="MSNLTSWDWWLATYFLAVGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            12962..13036
                     /locus_tag="ACHUNN_002759"
     tRNA            12962..13036
                     /locus_tag="ACHUNN_002759"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12994..12996,aa:Asn,seq:gtt)
     gene            13042..13128
                     /locus_tag="ACHUNN_002760"
     tRNA            13042..13128
                     /locus_tag="ACHUNN_002760"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13076..13078,aa:Tyr,seq:gta)
     gene            13133..13208
                     /locus_tag="ACHUNN_002761"
     tRNA            13133..13208
                     /locus_tag="ACHUNN_002761"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13166..13168,aa:Thr,seq:tgt)
     gene            13699..13785
                     /locus_tag="ACHUNN_002762"
     tRNA            13699..13785
                     /locus_tag="ACHUNN_002762"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13731..13733,aa:Ser,seq:tga)
BASE COUNT         4001 a         2933 c         3337 g         3597 t
ORIGIN      
        1 ttaaatttca gttgatattt aaattacata aaattatgta aggggatatt aaaatagatt
       61 gctcatggag gaagcatggc taatgacgta ggaggcgtca ctcatgatca ggttgagaga
      121 atagctaaaa ttgtcgccag agaagttgtt ggtaaattag gaaaagaaat cagagaggag
      181 atagttcatg aggttcatga gcagttgaaa aactattttg gtgacatgac tccggctcaa
      241 cacagtattc aacattccaa tctggataaa ctacttaata ggttagattc gatctcaagc
      301 ggcttctttg gaggcattat ttctaaagtg acgtcgttta ttataaccgc actaatttta
      361 ggattagccg catatggcgt aaaaaatgga ctgcaataac aggagaacaa ggatgaagac
      421 tccgagaggc attcgtaata ataaccccgg taatcttgat aaaggatcac cgtggcaagg
      481 tctggttgcg aatccagacg aacctcgctt ttgcacgttt aaagaccctg tttggggtat
      541 tcgtgctctg gcagtgactc taattaccta ccacgataaa cgtcgcgcca aagacggctc
      601 aagtattgat accattcgcg aagttattga acgttgggca ccgccgaatg aaaacaacac
      661 tgacacctac attaatgagg tgtctaaagc tgttggtgta accgcagaca tgatcatcga
      721 tctgcatgat tacgacatcc ttcgaccttt ggttgaggca atcattcgcc atgagaatgg
      781 gcgaggtccg ctaaaaacgc tgaacacctg gtatgcggca gaagttattg aggaaggtct
      841 gcgtcgagct ggagtcgtta agccggtgaa aaccgtgaag gctgttcctg taactaaaga
      901 aacagcaggc gcaactgtta cagcaggtat tggtctggcg cagctggccg atgttatgcc
      961 gcaggtttcc gctgctatgg ataaagcaca aggtcatatc tctagtgggg atacagtacg
     1021 catcatcttc ggtattgcca ctattgttgt ggcaggattc attgcctggt cgcaggtaag
     1081 aaaacaccag aaagggatgg cctaatatgc taggtagcct gatgataaag ctaaaagttg
     1141 ctttgattac tctggctgcc gttcttttcg ttctggtcgg cgcttacacg atgggcggaa
     1201 atgcggcgcg acgagcaatg gaagagaagg caaaacagga agacaggaaa cgacttcaaa
     1261 gcacagtgga tgtcaaaaat gaaacacttg atgagttacg gcgcaaagat gcttctactg
     1321 ttcatcatga gttgcacaat aagtggttgc gtgattaagc ctcaatcctc tggtgtgctt
     1381 ttctgcgatg cggctacacc gctatacatc agccgtgatg atctcatgac cgaagagact
     1441 gaaagggaag tactttttca caatatgata ggggagcgat tgtgtggatg gggcagaaaa
     1501 gtgccataat aaaagcggaa ttttctaaca taaaatttgc atattattag agcacaaggt
     1561 agatttgaag catggatgtc aagtaaatca taccttgttt taataaatcg acgcatactc
     1621 gtagcctcta aaccgacaag gtcgccgcta tgacataaag ttattaccgg ctaaggtgga
     1681 aacatgcata ctgcgcttaa cgcatctcgt tctattcaga cgataagaga ctgtaccaag
     1741 atgatcgcat cggttccgtt gcggcattgg aatgactcgc tccaaattcg tgaaatgaac
     1801 aaaccgggta tgcttaagga tcagcgtttc atcgttaacc ttaaaagtga cgttgaagaa
     1861 ggcaagttta ccatcaaatt gtttgcagaa atgatggtgc tcaacaatcg gttacgtttt
     1921 cttgttaagc cagaaaaaca gtttcccgat gttgtgcgtt gtgaacagga tgctttctat
     1981 gagattgaaa gcaaaatagc taaatttatc aaagataaat accgcgtaga tgttaaaata
     2041 gcgaaactgg attcatctac ggatatgcgg cttgctaact aaatgtcacc atcattcata
     2101 gcagagtatt caaaagtact gtctgatttc cctgaatgtg aagcaagaga gaaaatcaga
     2161 gttggctaca tggttaactc tgatttttct gttttacggc tcacagagtg gtccttgatc
     2221 gggtctaatt atgattactg tttgacagtg tcatttgtag gcgaccctga tgcagattgg
     2281 cttcaagaac atgcaatgga tcttgacgag caggggcatt cagaagaaga gatagttcat
     2341 agtgttcgtg aattagttgg tgatgagcaa gaagatgatg acggcattca gatcgccagg
     2401 ttcgtctaca aacgattcca gtttcgctca caaaaaggct ctcaccttgg ggtccagatt
     2461 aaaggggcat ttgtaacccc ttcaaaggaa caaaagggac ttgctagaag agtttacaat
     2521 tttcttctca attggcatga ccatgtagta tgtgatgacc atcagacggt ttacggtgcc
     2581 agaatttggg ctgtaggtat gcttttggtc ggtcgtgtcc aaatctatga caatttaaat
     2641 aaagaattta tagatgtcct tgttgaaggt ggtattgggc agaatggcgt caaaccgtgg
     2701 gatgcgctgc tattgaacag cgaggctcag ttacgtcact ggaaccctat ggctgtcagc
     2761 atagacccat ctagtcagat tctcaccatc atctccaaag aagaccgcca cattgaagtg
     2821 ggcgttacaa catttgatgc caggtctacc aaccactaat tcctaaaccc gctacggcgg
     2881 gttttttgtt attaagtcct taccaaatct atgtacctat cattaaacct ttacacagca
     2941 gccgtaggca tttaggctat atcacatata agaaaacaag ttgtttcaga caataataat
     3001 agacgcaaag gaaactctcc aatgaccaag atctttgtgg ttggcggcac aaagggcggg
     3061 cctggcaaat ccaccgttgc ccagcaaatt gccgtttgcc tgaaagtcaa aaagaagaag
     3121 aaggtttata ttaccgatat agatattcag cgcacgacaa cgagctggtg tgaagaccgt
     3181 cgacagaacg aagaccttga gctgattcct tttgcatacg ttcaggatga catcattaag
     3241 cacctaaaat cgcttcaggg tagagctgag tttgtagtgg tagatgctgg tggcttcgac
     3301 tccgaaattc agcgacaagc gatgctgatg gctgacgtta tcattatccc gctgcgtcct
     3361 aagcgtcgtg atttgaaatc tttacgtgac atcgatccta ttatcgacaa tgttcgcaat
     3421 gtaaacgata aagtgaaggt ccgcgcggtc atgaaccagt gcccggcttt gccatcacaa
     3481 gtgtctcgca ttctggcggc taaagagatt gttgagacgt ttggcatcga gtctgcgcca
     3541 gtcaatctat ataaccgcaa cgtctatgat gatgcggaag agtctggtcg ttctatcttt
     3601 gaaatgaccg gtagcgagcg cgacaaaaag gcggaagccg agtttgaaga atttgtagat
     3661 tatctgttga gtctggagga agaagaataa tgtccatgaa aatgggtgac ctagcaaagc
     3721 gcaaagagtc tgatgcaccg gctaagaaca caactccttt gcgccaacca gtcagaccac
     3781 agggacgccc gactcgtggc aaagagaaaa ttaaaagccg cacaatgtca ctggaggacg
     3841 aatacttcga actgctggag atgatgaagt tcatccctcg cttcgagaaa ttcacccgct
     3901 ctgatgtgat tcgtgctgct attttccatc tggcagagaa gtcaccgcag gaaatcgagg
     3961 acatcgtgaa gcttaacgag gcgatcactg ctgccgatgt cacgatgcgt accgatgaaa
     4021 ttaaacgcga gttaatgaag aaaggttgat ataaatctct aaggctggtc tgcattggac
     4081 agccttagaa atatttcgtt ctatgaaagc tattcgtagt taagctcacc gaacagactg
     4141 aacatcaagt caccatccaa aatttcaaag ggcaactcaa catactcatc cttatgtctc
     4201 ggaattgcat tgccgtcgtt aggggcattc ttggtataac cttttacatg tttaccgttt
     4261 tcttttgtat acggcagcac gacaattcga ccgtgatttt ttgaaatgat agttccttct
     4321 ttccagagcg tattgcctct tgaggtggtt tcgctttcgc tagcaacttt aacactccaa
     4381 cctacctgat ttttgtcctc aaaccagaaa acaaagttgc caacaaccag taccttttgc
     4441 ccattcctga gagcttcatc cagcattctt tttacgctgg cgagttgcag taaccggtta
     4501 gcacgcggca gaagcattga gcgaatagtc gcttttgttt ttccccaatg tgcagcacct
     4561 gataaaccaa aacctctggc tatgcgttct tggtagcaga tcttcgctgt cttagctttg
     4621 ttgttatatg aacattccca agtgttagga gcaacgagtt gaagtgaaac ttggaatttg
     4681 ggaagaaaaa caaaatgtaa gtaaggcatc tcgcttagca cgtcaaaaag gggttgacgc
     4741 aaagtttcgt aatctgggtt aattattaac tcatttaatg tcactcgcgg atcgttggcg
     4801 tgtgccttaa ttgcttcgtt gagcattata tactcttcat ccatgagacg tgatttggct
     4861 ttgagtgctt tgtcaacttt caaacgcaat gacttctttt gaaacataga tagaggaagt
     4921 ttttcaatgt caaagctcac gtttttattc agctctaatg ccgcttttct tgcttcagga
     4981 tagttatcgt agtcgtcatc aacagcgtgg tttggcatac cacgatcaac atatcgccga
     5041 taccaagcaa gggttccatc attacggagt ttcagacaga acatcgacca tttattccgt
     5101 tcatatttag gctgaggcca aaaagccagt tcgggaaggc catatcctgg aagtctattc
     5161 atgcgcatgg tgacaatatc cacgaggcta aaacactaat tctaacatga tctcccttga
     5221 taaatctgtc aatgacatag catctggagt tggagtcaaa ttatgttgtt cttggtatcc
     5281 attaggagcc tgtttctgta tataaataat aagtaactta ttaaatatat acggaagcag
     5341 gtcttttaaa agacaccacc agaacaactc ccttccgttt tcacttccaa aaactgacac
     5401 cagtcgctat catccgtcca ttgtgataag taagtaacta cctaccaggt gagccacatg
     5461 agccaaatct ttttcgatac catcgacaac gaccagtacg acttcatgac agagtggaat
     5521 accgctgtta tggacaagtg ggtcgctgaa aacattggtt tgtcgcgctg taaagacgag
     5581 gctgaactct tcgagacgaa gtggtttgat taccgagaca tgcatcctct tatggccacc
     5641 tgtctgttta cggaggcata caaacgtcag tactcaaata tcatgctgac gcacggtcgc
     5701 gaacactttg aaacagctcc gttcaccacc gggttaaaac gcctgcctta tcaggagttg
     5761 tcgactgcca ataaaacgtc tctatggaaa gcacgccagt ttgctgaccg ctattgctgc
     5821 tcatacgact actttatctc caccgttctt tccgcagctg cacgacggct gtgggacaag
     5881 ctgccgcgcc cacagcattt gtggcagcca gagctggttg agatattcga agagaaatta
     5941 gccagacgcg caacaacccg tctggatgac tctcttgtta gctttaagca tatgggagac
     6001 atgcagttca acccgattca ggaaagctat tttgagtgga ttctggagtg tttgcgcacc
     6061 atccctcgca gcaagcgcat acgcgcaatt ttctccgcta tctggctaat ggaaatcgtt
     6121 ccagagcgcc ttatttccgc ccactttcca gaagaactgg aagaagcacg gcggtttatt
     6181 gatcccctat ctaattaact aatactagaa aacaatttgt ttaaaaaaca aaggaaagca
     6241 catgacagaa ctttgccata caggacgcgg gctgtccgaa gagtttgatg aagatttcca
     6301 gaacagactg acggcatatt tttgtcgtga tcacgagttt cttactcgtg cgggagatct
     6361 ggttgtgcct agccaatttg ccaatgcggc caatgccata ttggttaata tggtttcggg
     6421 ctattaccgt atgtacaaga gcgcgccctc ttcatctgca attctggata tgcttaagcg
     6481 tgcgaaacgc gataagacta tccgtgagga actattcgcc gatgttgttg ctgcgtttaa
     6541 gcgcattctt gcagaaaaat tgtccgatac ctcgtacatg gttgaccagg tatcaacctt
     6601 cgcaaaaagt gtagcgtttg atgatgctct gattaaggct gctgaactga aagagaaagg
     6661 cgactttcag ggagcgatgg caatcatggc taaggttcag cagattggat cgaacgaagc
     6721 gaccggaatc tatgactact acacctccgc aagtgagcga ttgaaagcgc gtgaatatga
     6781 ggcttcagag gagtatgtgc caaacagcat tacaactgga ctccctctgc tcgataggtt
     6841 gctgtaccaa aaaggctggg cgaagcgtga aatggtgctc ttcatggggt tcgctaaatc
     6901 cggtaaatcg accgcaatgg gtgagttttc cataaacgca acgcttgctg gctacaatgt
     6961 tctgtatctc tcgctggagg ttcacaccac cattttatcc gaccgttttg atgcaagatt
     7021 gtcggagaca gaaatgtcca agctggtgga acggcgcgat gaggttcatc gtaagttggc
     7081 agagttggga gccacgaagg ggattggtag tttgtgggtg gttgagcgtc cgtcaggaag
     7141 tatgtcaccg gcagatctgg accgtatgct taacagcatg aaagccaacg gcatggtgcc
     7201 tgacatggtt gttgtcgact acgcagattt gatgcgtgcc agttatgacc ttcgtgatga
     7261 tcgcgccaac attcgtagta tctacaccga tttacgtgct ctttatgaca agcataacgt
     7321 tgctgggatc acggcatcgc agacaaaccg tgaaggtggc gcgtcagaag ttgccacaat
     7381 gatgcacgct gccgacaaca tcgaaaaagt acgtattgct gacctggtaa taacgatcaa
     7441 caaaaccgaa gaagaagaag cgaaaggaga ggctcgtctc tactttgctg gttcacgtaa
     7501 ccagcaggga gggatcagca ttcgcgttaa acaaaacctc gaacaaatgc gcttcattga
     7561 gcgaatctta gacgttacct aaaaaataag cgtggagaac acctccacgc ttgattcatt
     7621 ggtgaaacaa cttttctttt gccaaaccac aaaagaaaaa cacatgagcc tttatgttat
     7681 atcaacattt agattggtca caatattgcc tgttaaaagt ggaattatcg tgagcgagct
     7741 gaaagagcta attgccgaat tagattttga acaatggttg gatactgaag gtatcgttta
     7801 tcgacgtgga ggcgtgagta ctcgcggtcg tgaagtgaat atcaaggagt gtccggtatg
     7861 cggcagctcc aactggaagg tatatttcaa tctgaccagt ggcgtcggca aatgcttcgc
     7921 tggtgatcat cccgaagaga ttcagttcaa taagctggtc ttcctcaagc actacagcgg
     7981 taaatcacga cgacagttcg aggaatatgt gcagaacgct cttctttccc aggggtgggc
     8041 accaaagaaa gaagagctag tgcttgcaag cacagtcgag ttagaggggc cagttgcact
     8101 ccctcgtcat tacgagcttc ctatagatgg ccgtcttcca gattatctgg ttgaacgaaa
     8161 catatcacct gaaatggcaa agtattttga cctacgatac tgcgtcgaag gcaagcacgc
     8221 ttatgtagat ccgtatacag atcaggtaaa agggcagata ttcgatatgc gaatactgat
     8281 accggtttac gatctggatg gggtaatgaa gacatttcag ggacgagaca ttaccggtac
     8341 agcagaacgc cgctacctct ttcctatgca gcttccagct tcaggtaaat ttctctacaa
     8401 cggccataat gcagtcggca aacagactgt agttgtctgt gagggtgcgt tcgatgttat
     8461 gggggtcaaa cgagctattt tcgacgaaga aacattacgt gattacgtgg agccaatagg
     8521 aacgttcggg atgcatctat ctggcaacac cactcaggat gcagaagatc agttgggcgc
     8581 gttcctgacg ctcaaggcgc gtggattacg taatgtgatc atgatgtggg atagtgaaaa
     8641 gcaagctata cgcaacacga tggccgcagc caggcgactg accagtatcg gtcttaatgt
     8701 caaagttgcg tgtttgggtg aggaaggact cgacccgggc ggtgcaacgc cagaacaaat
     8761 tatcaaagcc tattatcggg caaaaccgta taccaaacag ttggagttgc aaagcaaggt
     8821 tttgggcatt aaggctctat cataacaatc acctttaaaa taagtagatg attacttatc
     8881 tttctgtgag aatactttca tctgttagct aggagttggt atgaaagacg aaattcagaa
     8941 attagcctgc gacatcattg ataaaactgg tttagaaatc agcgagagca atcgactaga
     9001 catcattgaa aaagcggtaa aaacagcaat ggatcatatc gccactcgtt tggtcgagat
     9061 cccgctaccg gggctacctt atctgaaggt taagttacac gtatggggtg aaccttcttg
     9121 tgcacgacgt tctgcattag ttgtttttat tagcaaagaa aacccgctca gtcttaaagt
     9181 gcaggttgga gcatggcttg atggcagagt gatctacaca aataccgttt tttgtctttc
     9241 aaacgacgaa actattgaag cggccattca agaagcagtt ctagcaatgc gcagcagcgg
     9301 tttgatgaag aataactacg aagagtactt gcgttcgata agtggtgaaa agacattatc
     9361 tctgaaagca gatttcgtta ccccgaaaaa tctgttggaa gtcttgctta ataaaggggc
     9421 taatgatgcc gtaaatgtaa tcagagagag tgagtatgcg tctctttgcg acatgtgcaa
     9481 aagccagttg gatctggtgc atatcgttat tgatgctggg aaggcatgtg atggcgtaat
     9541 ggcggaattt gcttggaagg tggtcaggat tgctaacgaa ttaccgatga tagagcaaga
     9601 ggctaaatca tacgccacca atcatgtcac agagcttctt gccccctatc gcttagaaag
     9661 caatcagcgc aagatgatta gctggggaag ttggtaatct ctccgcgcgt cgttttttac
     9721 gcaaataacg ataggtaagt acaagattat ttatggcggt agttgtgaaa gctgatttgt
     9781 caaaaatccc ctctatttca ggaaataatg gttattcact tcgttgtgag gaagtaaaga
     9841 taaacggtga gtcggcatat tgcagctact ccgtttgcca gcacaccatt cttgccttca
     9901 aagaaaaccg tcttcctcga acttcattcc agtcgtgcgc aaccgctatc aaagcaggca
     9961 aatgcaaggc gttaaaaatg atggttgaag agattcgtaa aggagaatct ctgtatttcg
    10021 aagatatgac cgcgctcatt aaggaggttg aagaacggaa taaacaagcc agaactttaa
    10081 aacgaaaacg tgacagtgta acgattaata gcatggttaa gaagagcacc acatcacaaa
    10141 cagcgatcac tgacgtgtat gcggcgttgc ttgaagaaac aacaaaagaa acacatgagc
    10201 aaatcgatca acatatggag gtaaaacaac aatgaaaaag ttgatcgcac ttaagcataa
    10261 gctggacgaa atgaaagcta tgggaaccaa tgcaaaaaaa gaggcattgg ccaacatgga
    10321 tgacttcgag caaagcatgg tttcattgat gctcaaccct ttcatccgtt ttggggtaaa
    10381 gaaatacaaa gtggcagagc cgcttagtga gtccatccca agtgacgaaa aagccattga
    10441 tgtactgaat aagctggcct ctcgcgagct aacggggaac gcagcaatag cagctgttga
    10501 gtctatcgtg gcgtcaatgt gcgccgatgg gcaggacgtg ttccgtcgtt tcctcttaaa
    10561 agacccgaaa gcaggtgttg ggattagcct atgcaacaag gtttttgaaa atcccattcc
    10621 gaaattcgag gtgcagctgg cgtcaccgta taaagaaaaa ggcgacaaat accccttcaa
    10681 gccaaatcct aaagcaaaat ggccgatgat tggcagtctt aagctcgatg gtttgcgagt
    10741 aatttgcgag gttattgttg acgaggaaga ggttaacttc ctttctcgta ctggtaatcc
    10801 aatcacgtct ctcgatcacc taaagccagc aatgctagaa ttaggcaaac tttcaggcca
    10861 caaacacatc ttcttcgatg gtgaaggaac tgccggttca tttaaccagt ccgtatctgc
    10921 attgcgcaaa aagaacgtgc aggcaattgg cgctatttat catgttttcg acttcttcct
    10981 accggaatgg cgagcacagg ctaaatccaa agagtatgca aagacaggta tgaagctgaa
    11041 agagcgcctg gctatgctgg tggcgttgtt taaaaacgat cgcagtgaag gctacacaca
    11101 agacattcac ctgcatccgt tctatatcat ccatagccac gaagacttca tcgaacgctt
    11161 catgaaacgc ctggacgata acgaagaagg ggagatgggc aaagatccga actctgttta
    11221 cgagtttaaa cgtacccgca gctggtggaa gttaaaagac gaagattcag aagatggtga
    11281 aattatcgat tttgagccgg gcgacccgga ctctggtttt gccaacacgc ttggaaaaat
    11341 agttattcgt cttgaaaacg gcgtcatcgt tcgtgcgagc ggcattaagc ataaatatct
    11401 ggacgagatc tggaacaaca aagagaagta ccgtggtcgt attgtcgagg ttcattgtca
    11461 cgagaaaaca cctgatggca gcttacgcca cccacgactg aaatggccgc gttgcttacg
    11521 cgataccgaa gatcgaatcg gagataaaga atgatcgtat taagtaaacg ggagaaagaa
    11581 acgcttcatg aaataagtaa gtggccggag ttccctcagt actggaagcc taaaacgcga
    11641 gctaatttag agcgtttagg gttggttgca aacatttcgg aaacgtggtg ctcagccaac
    11701 taccagttaa ctgataaagg gaaagtattg ctacagcaat tagtagaatc aggagtgtta
    11761 aaatgattcc atacatctca ttagctttta ttggtggctt ccttatcggc ttcggcatct
    11821 gtcgtgattt aattaagcag gaacttaaaa ccaaaacact gtgcatcgga aagcgtgtgt
    11881 atcgggtagt tcatgaaaca aaggttagaa aatgagcaat ttaacttctt gggactggtg
    11941 gttggccacc tatttcttag cggtcggagt cgcattcgcc ttttacgtag gtcagttagt
    12001 cgtaaaactg ttgctgatta aatttgctag tcataaacgt atcgatgatg gtctgtggcg
    12061 tcttggcacc ctggtggaaa ctcgctacgg gcaacttaag gagaacgaaa ccattactat
    12121 ccaagcgaaa cgattcactg ccaccatcac aagaacacct agtcgtagag tggccttgat
    12181 caaaaaagtc acaaccgaat aaaaacatat cgataagtat ttacttactt atcctttgtg
    12241 tataagatga ctttgttttc gttgagatgc gactgtttga acttaaatac aagtgcaaac
    12301 gaagaagtct atctggcagt agcctaataa gccaaacacc agcgaggtca gtttccagcc
    12361 tcgttaccga aatgggacac actgagcgag tgtgattgca gaacgcagga gggaacactc
    12421 atgttccctc cgatgaagta acagaatggg cggtcggtat attttcaact ccatatgact
    12481 cccggattct tagccactga ccgcccattc tgttacgtca ttttgttcaa ttatgtcgtt
    12541 tatactgggt taaaaagcgg cgacgtagcc cggctggtat ggttagccag cacacaacgt
    12601 tgaggccatt acatttttat caattctaag gttctattca cagagatacc ggcgagcgtt
    12661 gatatgtaac atgttgggca aacattcaat cggagtagtg gcctcaacgt tgtgaagaca
    12721 ggattgttgt gtaggtttaa ccacttttcc acaacaaatg attccataca tcacattgta
    12781 taaattacaa agtaggtgct gtcctcagaa acatcatcta cttaaagatt ttgccttcta
    12841 ttgagagaag tcgaaagcgt ctgacactaa cgaaaagtgc aagtagcggt gcgtttcctg
    12901 gcagaaacta acaccgccgc gattggcact gttgagtaat aaatactggc agtgccgaat
    12961 tgatggtgta gctcagcggt agagcagttg gctgttaacc aattggtcgg tggttcgaat
    13021 ccacccacca tcgccaattt aggggagtta gtccgtagag gtagcggggt agactgtaaa
    13081 tctactgtca ttgcgactcg ggtggttcga ctctatcact ccccaccaaa ttgccgattt
    13141 agctcagttg gtagagcagt cgctttgtaa gcgaatggtc atcggttcga tcccgttaat
    13201 cggcaccaac ataatattga gaacattggc gtaacggggt catatcccaa tctatgaata
    13261 aatgttgcgt tgccgcgtga caaccagtgt tctcaacatt gtggtgaatg cacaggctga
    13321 tgtgccgcaa ctacagtagt gcgcgctttg cggggcttgc tacaaccctg tgtcggagat
    13381 cagcgccgac catcacaatt tggctctctt gcaacagcat aacgctgaaa tatgtccaac
    13441 ctggtgcggc ctgttcaccc gccgttagct ccacgaaacg gagcgcacaa caggtaagaa
    13501 cattttcaga gttcgactct ctgagttgcc attcgtggtg gcacgcgaga cgtgaatgct
    13561 ctttccgttg tggttgatgc gcagactgat gcgcggaaag aaatgcccca gcgacacacg
    13621 aggcgagttc acacactgca atgcagtcag tagccggggt cgctaagccg gagatcagta
    13681 ccggtaacca caacgactgg agagtaggga gcatggtgct caagcggtct tgaaaaccgt
    13741 cccattgcgc aagcgatgat ggttcgattc cattactctc cgccagacac agcgttgagc
    13801 ggtttggcct tttaatcaac tcgattaaga ctccgctaac ataaaccaga ccgctcaacg
    13861 ctgtgtgt
//
LOCUS       DAZOXR010000108        13808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZOXR010000108 DAZOXR010000000
VERSION     DAZOXR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.110"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUNN_002763"
     CDS             <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUNN_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0577093.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="ACHUNN_002764"
     CDS             complement(984..1337)
                     /locus_tag="ACHUNN_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0577094.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUNN_002765"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUNN_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0577095.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="ACHUNN_002766"
     CDS             complement(3165..3458)
                     /locus_tag="ACHUNN_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0577096.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUNN_002767"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUNN_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0577097.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /locus_tag="ACHUNN_002768"
     CDS             complement(5012..5482)
                     /locus_tag="ACHUNN_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0577098.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUNN_002769"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUNN_002769"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0577099.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUNN_002770"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUNN_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0577100.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUNN_002771"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUNN_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0577101.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUNN_002772"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUNN_002772"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0577102.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="ACHUNN_002773"
     CDS             10838..11413
                     /locus_tag="ACHUNN_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0577103.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="ACHUNN_002774"
     tRNA            11520..11595
                     /locus_tag="ACHUNN_002774"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            11838..11952
                     /locus_tag="ACHUNN_002775"
                     /pseudo
     CDS             11838..11952
                     /locus_tag="ACHUNN_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12212..12511
                     /locus_tag="ACHUNN_002776"
     CDS             12212..12511
                     /locus_tag="ACHUNN_002776"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR0577104.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUNN_002777"
     CDS             complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUNN_002777"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR0577105.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUNN_002778"
     CDS             12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUNN_002778"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR0577106.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
BASE COUNT         3418 a         3496 c         3313 g         3581 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttccca
//
LOCUS       DAZOXR010000109        13807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZOXR010000109 DAZOXR010000000
VERSION     DAZOXR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.111"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUNN_002779"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUNN_002779"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR0577107.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUNN_002780"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUNN_002780"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0577108.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUNN_002781"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUNN_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0577109.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUNN_002782"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUNN_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0577110.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="ACHUNN_002783"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="ACHUNN_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUNN_002784"
     CDS             complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUNN_002784"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0577111.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUNN_002785"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUNN_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0577112.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUNN_002786"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUNN_002786"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0577113.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUNN_002787"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUNN_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR0577114.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUNN_002788"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUNN_002788"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0577115.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUNN_002789"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUNN_002789"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR0577116.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUNN_002790"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUNN_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUNN_002791"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUNN_002791"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0577117.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUNN_002792"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUNN_002792"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0577118.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUNN_002793"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUNN_002793"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0577119.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAZOXR010000110        13794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZOXR010000110 DAZOXR010000000
VERSION     DAZOXR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.112"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..147)
                     /locus_tag="ACHUNN_002794"
     CDS             complement(<1..147)
                     /locus_tag="ACHUNN_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR0577120.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="ACHUNN_002795"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="ACHUNN_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="ACHUNN_002796"
                     /pseudo
     CDS             476..1021
                     /locus_tag="ACHUNN_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHUNN_002797"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHUNN_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0577121.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="ACHUNN_002798"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="ACHUNN_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0577122.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="ACHUNN_002799"
     CDS             3028..3201
                     /locus_tag="ACHUNN_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577123.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            3227..3295
                     /gene="yncP"
                     /locus_tag="ACHUNN_002800"
     CDS             3227..3295
                     /gene="yncP"
                     /locus_tag="ACHUNN_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0577124.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHUNN_002801"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHUNN_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0577125.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="ACHUNN_002802"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="ACHUNN_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0577126.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="ACHUNN_002803"
     CDS             complement(5499..6422)
                     /locus_tag="ACHUNN_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0577127.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="ACHUNN_002804"
     CDS             6639..7982
                     /locus_tag="ACHUNN_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0577128.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="ACHUNN_002805"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="ACHUNN_002805"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0577129.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="ACHUNN_002806"
     CDS             10002..10226
                     /locus_tag="ACHUNN_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0577130.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="ACHUNN_002807"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="ACHUNN_002807"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0577131.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHUNN_002808"
     CDS             complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHUNN_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0577132.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="ACHUNN_002809"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="ACHUNN_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0577133.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="ACHUNN_002810"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="ACHUNN_002810"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0577134.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3483 g         3574 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAZOXR010000111        13774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZOXR010000111 DAZOXR010000000
VERSION     DAZOXR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.113"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHUNN_002811"
     CDS             <1..254
                     /locus_tag="ACHUNN_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001648036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577135.1"
                     /translation="NISQYVLDSCYKSGEKHPITICSAGETSTWWEDIWHEDMIAQSR
                     NNESYFWDGDKPLIDSLHKELQKNNRLKPNSELDMPLIG"
     gene            472..1680
                     /gene="trbA"
                     /locus_tag="ACHUNN_002812"
     CDS             472..1680
                     /gene="trbA"
                     /locus_tag="ACHUNN_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR0577136.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYTSCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPKKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            1699..2769
                     /gene="trbB"
                     /locus_tag="ACHUNN_002813"
     CDS             1699..2769
                     /gene="trbB"
                     /locus_tag="ACHUNN_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HFR0577137.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            2762..5053
                     /gene="trbC"
                     /locus_tag="ACHUNN_002814"
     CDS             2762..5053
                     /gene="trbC"
                     /locus_tag="ACHUNN_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR0577138.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(5090..7789)
                     /gene="nikB"
                     /locus_tag="ACHUNN_002815"
     CDS             complement(5090..7789)
                     /gene="nikB"
                     /locus_tag="ACHUNN_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HFR0577139.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRNERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(7800..8132)
                     /gene="mobA"
                     /locus_tag="ACHUNN_002816"
     CDS             complement(7800..8132)
                     /gene="mobA"
                     /locus_tag="ACHUNN_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0577140.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            8365..8700
                     /locus_tag="ACHUNN_002817"
     CDS             8365..8700
                     /locus_tag="ACHUNN_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR0577141.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTTQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKTSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(8786..9634)
                     /locus_tag="ACHUNN_002818"
     CDS             complement(8786..9634)
                     /locus_tag="ACHUNN_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0577142.1"
                     /translation="MNQTLPTTDLNTAGTTDVIPSVAIDRIITQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNACKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENMLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            9983..10180
                     /locus_tag="ACHUNN_002819"
     CDS             9983..10180
                     /locus_tag="ACHUNN_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577143.1"
                     /translation="MQGAPHPARLKKNDGTTMACGGHWHTTPAQVNTEKTARHLQPKK
                     VKFLHFFTLIYCVDLMIIMIK"
     gene            10214..10465
                     /locus_tag="ACHUNN_002820"
     CDS             10214..10465
                     /locus_tag="ACHUNN_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0577144.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(10496..10741)
                     /locus_tag="ACHUNN_002821"
     CDS             complement(10496..10741)
                     /locus_tag="ACHUNN_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577145.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFGEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(10741..11655)
                     /locus_tag="ACHUNN_002822"
     CDS             complement(10741..11655)
                     /locus_tag="ACHUNN_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0577146.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGALEKALAIAC
                     QLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(11720..11986)
                     /locus_tag="ACHUNN_002823"
     CDS             complement(11720..11986)
                     /locus_tag="ACHUNN_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577147.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(12080..12514)
                     /locus_tag="ACHUNN_002824"
     CDS             complement(12080..12514)
                     /locus_tag="ACHUNN_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR0577148.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(13243..13743)
                     /locus_tag="ACHUNN_002825"
     CDS             complement(13243..13743)
                     /locus_tag="ACHUNN_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFR0577149.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         3393 a         3677 c         3450 g         3254 t
ORIGIN      
        1 ataacattag ccaatatgta ctggattcct gttataagag cggagaaaag caccccataa
       61 ctatatgtag tgcaggtgag acaagtacct ggtgggaaga catatggcat gaagacatga
      121 tagctcaatc gaggaataat gagtcctact tctgggatgg tgacaaacct cttattgata
      181 gccttcataa agaacttcaa aaaaataaca gattaaagcc aaacagtgag ttagatatgc
      241 cactaattgg ttaaacacca attaaagatt atctagctaa aacaacatcg tacacgcata
      301 ctgcaaagcc cttggtgagt taatagaaga attactaagg gcttataaaa aatatatacc
      361 cccacattgg tgacacatta aaatattttt tgttgatgtg ttaccagcct gacccaaaaa
      421 caatttttaa tctttccgtt cgatttaaat tccagaaatg gagactttgt catgtcttac
      481 aatcgacagc ctgtcgcaga agatcctatg cagatatggg gcgctgttgg tgtccttctt
      541 atcctgctgc tattcgtcat atggttattc ttgccggaag tggtttacac cagctgcctg
      601 attcttcata ccttatgggg actggtcgac tggggaccat ttcataacta tgcagcacca
      661 cgatacaacc tgcttgccat gaccggtaac aacgccgcca acataagcta tagccagtgg
      721 gttaacgtca tggaacagac cataggtatt ctgtggatgt acctcttacc tgtgacactc
      781 tggtgtttgt gggaatggta tcaacatccg ggacaaagcc gtttcacccg cagaccagta
      841 gatattaccc gactgccaca catctttgct tcgttatccc ctgctatcgc ccctgtactg
      901 gcagatggtg atccgaaaaa actctttcat ggtggtaaac gacctgaacg gcgggttgct
      961 ctcaccccgg aagcatttgt cgaacagcat actctcatca ccaatatgca gctcgatgtt
     1021 gcagctgcaa gacgatgttt catggcccag ttgggtaaac ctttaacgtc ctggaaagac
     1081 atggcaccac atgaaaaagc gctctttgcc atatttggcc ttcagtattt cctggatgat
     1141 cggaaggcag cattaaagct catggataca ctgaacctct catgcagaat taaaagcaaa
     1201 cgggacagcg ggaaattctg tacccctgtt tattcattgg ccaaatcagc tttccagaga
     1261 gtgattaaaa gcaacggagc acagcaatgg ctgaaacaac atcgctacgt ccgcagtggg
     1321 ctggtctggc tgtatgcaca tgacctgcgg cttacgccac ccaactggat ttggctgaaa
     1381 ggcgtggaca gaacgctgtt ttatgcatta caccgcgcca ataccacgaa aggatttatt
     1441 gaaggcgctg gcgtggtggc cgttgcacgt gcagaggccg aagccatgcg ttttggtctt
     1501 ccctgccctg aaccctgtgt tgatgaagcg gtggaaggct tacggcggga tatgctcagt
     1561 ctcggtctta tctgggatga accacaacca gatcgcgacc ggaaacgccg catcctgacc
     1621 aactggtccc tcaccgacga catcctgccc cgcacacctg ctacagacaa cgaattctga
     1681 ttatccggag aactgaaaat gacaatcgag tactttgcaa cacgcattgc aaaacatatt
     1741 tccgtgcagg caatctggcc ggatggcagg acagaaatta ttgccgtcct tccacgggat
     1801 gccctgtcag ggctggtaac acgaaataat caactgattc atatagcctt tgacgccatg
     1861 ggcacccgta acagagaaac cgtcctgatt gataaacagg agcataacgc tgaccagata
     1921 cttacagcta ttgattcctg cctgcgcctt gaatatctga tggaacgccg cttcagcaga
     1981 acacctttgt tccgtggcat aatcgccagc gttgtgctgt tcgtaatggc aacgattggc
     2041 ttctctctgt tccgctatgt tgatcgtgta ttctgggatg atacaacgcc tgaagcagta
     2101 cagacagcag gtgagccacg actgctaccc cctcacctga atcacactgt gccattaaat
     2161 gaaggcattc aactcccggt tcccccgaaa aaagacgtcc aggtcccgga gaaaacaatc
     2221 acaagcaaaa atccggaagc cgcagctgcg cgacataatc tggctactgt tctgaagcgg
     2281 aacgccgatc ggggcatgtt taccatcaac ctgtcatccg gtcatgaacg aaccctgttc
     2341 gcatttctcg atcctgcctg tcctaactgc agacttctgg agccggcctt aaaacgcctg
     2401 gccagcgact tcaatgtcgt tatctacccg gtttcagtta ttggtggaga ggaatccacc
     2461 gaccgtgtgg caccactgct gtgtgaaaaa gatgctcaga aacgtgctgc cggctggcac
     2521 cgactgtatt ccgcagataa cggaatgatg acgccatcag aagaaacaac gcctgcagat
     2581 gaaacatgcc tgaaagcagc cagagctgcg attgatgtta acaatgtcgc cttccgtaag
     2641 ttcggatttg ccggtacccc atgggtgctg tccgacaccg gctggcatct gcctacagga
     2701 attctgcagg aaactggcac gcttaattta ttcctgaaaa ccaccgacag cgagtctggc
     2761 catgagtgaa catcgcgtta accccgagct tttacaccgt acagcctggg gaaatcctgt
     2821 ctggaatgca ctccagagtc tcaatatcta tggcttttgc ctggtggcaa gtcttgtggc
     2881 cagttttatc tggccacttg cattaccggc atgtctgttg ttcacactga taacaatgct
     2941 ggtatttagt ctgcagcgat ggcgctgccc tcttcgtatg cccatgacgc tggaatgtgc
     3001 tgacccgtca caggacagaa tgattaaaag aagtcttttc agcttctggc caaccctgtt
     3061 ccagtatgag gtcattctgg aatcaccggc cagcggcata ttctatgttg gttatcaacg
     3121 cgtcagggat attggcaggg agttatggct gagtatggac gatctgaccc gccatataat
     3181 gtttttcgcc accaccggtg gcggtaaaac ggaaaccatc tttgcctggg cgattaaccc
     3241 actttgctgg gcaagaggat tcacccttgt cgatggcaag gcacagaatg atacggcaag
     3301 aacgatctgg tatctggcca gacgctttgg acgtgaagac gatgtggaag tcattaactt
     3361 catgaacgga ggaaaatccc gcagtgaaat cattctgagt ggtgaaaaaa cgcgtcctca
     3421 gtcaaatacc tggaatccct tctgttacag cactgaggca ttcacagcgg agacaatgca
     3481 gtcaatgctg ccccagaacg tccagggggg cgagtggcag tcccgggcca ttgccatgaa
     3541 taaggcactg gtatttggta ctaaattctg gtgtgtgcgc gaaggcaaaa caatgtcgct
     3601 gcagatgctc cgtgaacata tgactctcga agggatggcc aaactttact gtcgtggcct
     3661 cgatgatcag tggccggaag aagctatcgc accgttacgt aactaccttc aggacgttcc
     3721 cggtttcgac ctgtcgctgg tcaggacacc atcagcctgg acggaagaac ccagaaaaca
     3781 acatgcttat ctctccgggc agttttctga aactttcagc acattcacgg aagcattcgg
     3841 ggatattttt gctgaagatt ccggtgatat cgatatccgt gacagcattc acagcgacag
     3901 gattctgatg gtaatgatcc ccgctctgga tacatcagcg catacaacgt ctgctcttgg
     3961 acgaatgttt atcacccaga aaagtatgat tctcgcccgt gatttgggct accgcctcga
     4021 aggaactgat tccgatgcac tggaagtcaa aaaatataag gggcgtttcc cttatctgtg
     4081 tttccttgat gaagtggggg catactatac ggaccgtatt gctgtggaag ccactcaggt
     4141 gcgttcactg gattttgctc tgattttgat ggcacaggac caggaacgta tagaaggaca
     4201 gacaacagcc acgaatactg caacactgat gcagaacacg gggacaaaat ttgccggcag
     4261 gattgtcagc gagggtagta cggccagaac gctgaaaagc gccgccggtg aagaagccag
     4321 ggcgaggatg aacaacctgc agcgtcagga cggcatattt ggtgaatcat ggattgacag
     4381 cccacaaatc agcatcctga tggaaagcaa gataaatgtt caggagttga ttgaacttca
     4441 tcctggcgag ttcttttcta ttttccgggg agaaaccgta ccatcagcat cattcttcat
     4501 ccctgacgat gagaaaagct gcagcagcga tcccgttgtt ataaacaggt atatcagcgt
     4561 ggatgcgcca cgcctggacc gtctccgccg tcttgttccc cgtacagctc aacgtcgaat
     4621 cccgtcaccg gaaaatgtca gtgccatcat tggcgtcctg actgcgaaac cctccagaaa
     4681 aagacgaaaa attcgcacgg agccgcacac gatcgtggat acattccagc aacgcattgc
     4741 aggacgtcag gcggctatgg caatgctgga agagtacgac acggatatca acgcacgtga
     4801 aagtgccttg tgggaaacag cggtgaatac gcttaaaacg accacacggg aagagcgacg
     4861 aattcgctat atcacgctga acaggccaga actacctgaa acaaaagagg aaaatcagat
     4921 atcagtgaga gcagaaagag caggtataaa cctgttaacg cttccacagg acaataacca
     4981 cccgacaggg cgtcctgtaa acgggtttca tcataaaaaa accaaccgtc cggactggga
     5041 cggaatgtac tgaacagtgg ccgcgccgga ttctggcgcg gccttttttt taacctggag
     5101 aaggcggttc ccagcgttta tcctgctccg gcggaggcag tgatgcgtca tcgttgctgt
     5161 tcgccgcgat ataatcgcgg tagtagcgtt cacttccttc acggtgatga tccacgtccg
     5221 gtcgcgtaat cctgtagtct tcgtgaccgg tacggcctgt cacattatgc caggcaacca
     5281 tttcagcaaa gacctggtta aattgtttgt cgtttccttc tggttcaaaa cccatgcgtg
     5341 gatcacgccc gaataatacc ggtgcaatca aatccagttt atctgccagc gcattgcggt
     5401 catggtgatt acggaaaaca accctgtcac cataatccgt acagacaaac tctcccgtcc
     5461 gacgatcacg gaaacgaaca ctcccgttct tctgcaaacg ggcctcgaga tcaccggtat
     5521 taccgtatac cggcgttccg cccggttcac aaagcacgac acagcggtct gttgtcgttg
     5581 tgcgtgactt atctttacgg cgatcgcgat aatcccagcc tctcagctgc gatacagcag
     5641 ccctgtctcc ctgagcagcc tgaatttcga cccactgacg gtaagagggt ggataccact
     5701 tcccgtcagc aataagtttc tgccgctcat tcctgatgtc ttctttcagc ctgatcaatg
     5761 cctgcattcg ctgaacttct gcaatgtgat aatgcagctt acgtaatgcc ggatcatcgt
     5821 actgcgcacg aatgtgtgac ttccgcagac ggcatgcctg atgaatttcc cgacaacgtt
     5881 ccccataacg cagatctggt ttacgccatt gttcacgcca tgccagatag cgggcacgca
     5941 ggtcagcacg ggcctcagct ctggcttcac gtcgctgccg gcgcagcatt gggtcacgtt
     6001 tactgctgac cccgtacctg tcactgacag ccagctccgg gttatagcga ctttcaggtt
     6061 gcaccctgtc aaacaaatct gctggggcac tcacaaaagg tccggcctgt ggctctgcac
     6121 gccccagagt cagatcagga tgaatgctgc tggccttcac cggcgtttgc tcatgattaa
     6181 aggcatcaac gacaacaagc ccgtgatgtt gtttctgtag catcagtccc tgttgtgcaa
     6241 acaactcatg acaacgctgc caggtaaact caccctgtgt gatcatttct tcaatctgag
     6301 ggctacaatg gctgatgctg tatccccaca gactttcttt gtcagaaaac agttcgcgtt
     6361 tgatagcccc ctgcggcccc gtacctgtac gcatacggtg ccattcttta tcggtcgcag
     6421 gacagctctc catacgacga ccacggaagc gttctgttgg cgtaacctgg aggaaaatat
     6481 ctgttggtac aggctgccag ccaccatcga gttgattcac attgtcgagc gtcagcagtg
     6541 accatacgct gtcagcacga acgggagtct ggttatgatc ataaccgtca cagataacca
     6601 ggtgaccatg ctgaacacgc atccataatc ccgcttcagc cagtgtgcgg tgaatagcct
     6661 ggcagttttc cagccgtcct tcccgtgcca tttccggcag acgttctcca agatgacgac
     6721 aggcatactg ctgcagactt tctgtttcag cgattttttc cgggcgaaca tcagctgcaa
     6781 tgaagtccgg attatagcgt cctggtgctt ccacctggct gaaaatgtct tttggtactg
     6841 gcgtgtagtc acccattttt ttcatgagtt tttctgcaca ccaggaggga ccaaacgagt
     6901 caagctgtac accttccctg ttgcgatccc agccatccat caccagaaat tttccgtcca
     6961 tctgagacag gtacagacca tcttccgcaa gacgacgatg cagggataac cagtcattta
     7021 caggttcact gcgtaaacca gcgaccgctg tctgcgcgac atactcgcga aaagtttgtt
     7081 tttttccgcg tgtccaagcg ttctggcgat cacgttcaac ggcagttttg cgaacgatac
     7141 gatttcccgg cgcatggacc cagcaaccgt tatccggagc aaaaccatgc ttcagttcaa
     7201 gctcacggca ggcacgactg agtttttcct gactccatga taatcggttc agataacccg
     7261 tttcagggtg tacccggtta acagccacat ggacgtgcag attatctgta tcagtatgca
     7321 cggcagagac atactgatgg tcggcaaggc cgagtgattt cagcgtatga cgtacactgt
     7381 cataaatctg ttccggacgc gggctttcat gtgactgcca ggaaaggatg tagtgaaaaa
     7441 caggatcagt gtcatctttt gcatagtgtg cctgccgggc aatgtactcc atatctgcag
     7501 cggcagtttc aagagaagta cagttatgaa agcaggtgac accataaaag ttgacccatt
     7561 cgcagccgtc cttcatgaca tcaaccagcg ccacgaatga ctcatttcga agacgtgtcg
     7621 cataatcaac aaggcgactg aaacggcttc ggtgcggttg ttcagcctgc gaagaagaag
     7681 ataaatccag ttcctcatcg gtcatgtcat cccggacaga gacgtaggat accaggtctt
     7741 cgaacgaaga cttgccgtcc cttctctttt tcggaatgac tgcattcacc ggagaatcct
     7801 caaccttcca ttaactgttt acgcaatgta tttgtaagtt cggttatggc aacaagcact
     7861 tctgcgtatt ccttgtcacc ggttcttttt ccctgaacaa acagatgctt ctgcatcctt
     7921 cccagtctca ttaactcctt cagaaaagca tcatctgtcc gggagttaat tcgcttattc
     7981 agtgctgcat ttcggatgta agcagataca gtaagtccgg aatcttcagc attcttacag
     8041 atagtttcat cctcatccgg tgagaaccgg accgctctca ggatcgtctt ttgccgtctt
     8101 tcactaccac ttctttgacg tttatcactc acggtcagct ccttactggg gtgtcggggc
     8161 gaagccctga ccaggaggca attgtataat cgcgcgtgcg cggttataca attgcacatc
     8221 ctgtcccgtt tttcaggcca ttatagcctg ttatttttgt gcatcatagt cttatgcaga
     8281 cggcagaggc agggcgcagc cctgccagga gtcagttttt gatgataaaa tcaggcttca
     8341 acattaaacg gagagaaagt gccaatggct tctaaaaaat tttactcaga tgatgacatc
     8401 cagctggcaa aagctgcttt atcggaattg ccagatctca ccactcaacg gaaaacattg
     8461 cgtgattttc ttgatgccat tcgtgatgac attattgtcc tggtaaggac aaagggttat
     8521 acactggcag atgttcgtga cacgctcaaa aatgcagggt atgaagtagg agagaaggca
     8581 cttcgtgaca ttattcggga agcagaatcg aaaaaaacat cccgccgttc ttcctctaaa
     8641 acagcaccta aaaagacaga ttcaggcagg aaagatggca tcgatatgaa caacaattga
     8701 tgtgacaatg tcacatcatg ttctgagatt atgatgatta cagaaaaaaa agccccggca
     8761 taaataccag ggcttttctg tttaatcaga ctcgtggcgg caatgcacca ggataatgcc
     8821 gtgcaataat atcattcagt ttttcaacaa ggtcaggacg cttaaagaca atgtgtacgg
     8881 tccctttctg gtagccacgc accgtaaaat attccccttc taacagctta ccgacgttgt
     8941 cgccgttccc gagaaattca ccatactgcg ccccgatgct gctccggaag tcaggaacgt
     9001 tgcgcccctc cagcagataa aaaggacgta ccaggtcatc cagtgcctcc cgtccgctgc
     9061 agtccagcgt cacacgtccg ttactccagc ggtacagcat gttttcaata atgattctct
     9121 tgcccagacg gcagggatta ttggttctgt aatcccatga caatttccgg tagacatcaa
     9181 tcagtccctg ttcaaatgtc tgcatcttgc aggcattcag atggcggaat gttgccagta
     9241 cattgtccag ggtgatttca ggacaggtgt cactcatcag ctggctattc cactcatcac
     9301 gctggcatga actcatgaag gtgtacatgc ccgtatctgt catcaggcgg tcccagattt
     9361 tccggtcggc aagacgggtg atattccttt tcgcttcttc cggtttatct atgcgacgaa
     9421 cggaatcagt cacgcagtct tcaaacccgt aaagaaaaat atcacctgac gcatcgagca
     9481 gaatcttgcg cgctgtcgcc aggcattcca tcgcctgcat gaacagtgca ataccttcgt
     9541 tacgctgcgt gatgatgcgg tcgatagcca cagacggaat aacatctgtc gtgccggcag
     9601 tattcaggtc agtagtgggt aaagtctgat tcatcacatt gtctcctcat cgtcacgact
     9661 gccgggtcag ccaggtcatt tcccggagga gcgaagctcc ttctgccgtc cgggtcttcg
     9721 gggatttccc cgcagtggac ctttccgacg ggggacgctc accacgtcac ggggcagttg
     9781 tccgggggcg gacgcaaaag tttttgacgc ctgcggcaaa aagttttgtg gctggtacat
     9841 ctctcccctg gacgactgaa acgggatgtg gtcgtgtatg tacccccgga aagtccgtct
     9901 gcggagaaca ggccgcagtg cggcggcaga caggcagaga cagctgcgcc atcaggcgca
     9961 gtggccttac acaccgtcag gtgtgcaggg agcgccgcat ccggcgcgac tgaagaaaaa
    10021 cgacggcaca acaatggcat gtggtggtca ctggcacacc actcccgcac aggtgaacac
    10081 agagaaaacc gcacgacatt tacaaccaaa aaaagtgaaa tttcttcact tttttacact
    10141 tatttattgc gttgatttga tgattataat gataaaataa agtgaagttt tttcactttt
    10201 tcaaaggtgt tttatgaccg gttgggaact gcgaatctgg cgaaaaagca tgctctggtc
    10261 acgcgaaaaa gcggccagag aatttggcgt gacgcagaga acatggcacg cctgggaaaa
    10321 tgcggaacag gtggacgtca ccgtctggag gaccacccag gctctttcag tccgtgacct
    10381 gctgcctcac atgcagggga tgcgtaaagc agatatcatc agacgactgg aaaatgaact
    10441 gggggaaacg gcagaggatg tgtgagcgtg attcacagcc tggctgaatg ccaggctact
    10501 gctcatcagg aaacagcttt ttcagttctt caccggaaag tcccgtgacc tcctgaatcc
    10561 gttccggcgg ccagcctata tcccgaagcc ggcaggctat ttcccgcgcc acttcaagag
    10621 ccccttgttt aaaaccctcg ccgaaacctt tctgtataag acgttctgca atcgtcatgc
    10681 tgtttgctct gctcctgctg acaaaaaagc ccggcatttt gccgggcttt gtggttctca
    10741 tcagtgagtg attttcttca gttcgtcatc ggaaagtccg gtagcctgct taatctgctc
    10801 cggcgtcatc cccattttct gaagctggca cgcaatagcc agggcttttt caagcgctcc
    10861 ttccatgcgt ccttccatcc tgccttcctg gcgacctttt tgctcaagac gttctgcgat
    10921 agtcatcagt ttatcctcat gctgcggcag acgctcagcc agcgcccgca taaattccgg
    10981 tgatacagag tcccctgcgt taacgatgta atgaatcaga acacgcacct gctcgtctga
    11041 aatttccacc atgacctgcg ggagtttgtc cagcagctcc atcatgtcac gatggcggat
    11101 atgcttcatc agcagcgtca gtgcggccat ccggcgatgg ttcatgattt cattgtcatc
    11161 aacgacagtg atatcaacca gcggaaacgg ctttgtgtat attttagccg caagtgccgg
    11221 attctcaaaa cagtccagcc agttcatgct gtacggatac gggctgcgct caccatgata
    11281 gaacagcacc ggaatcacca gtggcaacgt tttgtgctgc tccagatgtc tctgcatcgc
    11341 cgccacactg taacgtaaca tgcggaaagg catgtacaga ttttcggtgc tctggtgctc
    11401 gatgagcgta taaacatagc catcttcacc cccggtggtt ttcacgctcc agaggatatc
    11461 gctggcgtac tgatgcaggt ccggctcaac aaaggtggcg ggttccagct tcagcgtgga
    11521 caggtcgcac aactgccggt actccgccgg aagatgcagt tccagaaaat ctctggcgac
    11581 gtcgggattc gccaggaacg accggaacgc ggcatcatgc ggcgtgggcg ttgtggtgtt
    11641 tttcttctta ctcacggttc ctcctggtga gatgtctgaa cgtatcctgc cgcctgcaca
    11701 ggcagacggc aagggttaat cagtggcaga cccgctcaat ttcaagcgcg cggagcaggt
    11761 ggccccattc gctgactttc atagcgggga tacgcacccg cttctgctca ctgcagcgtc
    11821 tggcgatatg gccgctcatc gccaggacgt ccgggttaat ctcatcctgt gccagctcct
    11881 gacgtctgaa gcgggtatgg cggtgcaggg gtttattctc ccgcaggcac tcttcacgca
    11941 gacgctttac atactcggcg gttgcgaaga taattgatgg tctcatggca taatctccat
    12001 agatagtgat tgagatgccg gacttcctgg cggggaagcg ccggcggaga gaggcagggt
    12061 ttatcgccct gccttttttt catttcggac agcgacgcag tgccagcgcg agcgctttca
    12121 tcttccagcc ttccggcgtg cggtacatca ccttccagta gccgtcgatg aacacataat
    12181 ggaagcgcac ctcttcagtg ctttcagtgg caatagcctc cctgaaagaa cggtactcac
    12241 gcgcctcact gtccatccgg aaaccactgt cacgtccgta ataagccgtc acaccgtcca
    12301 ccgggttctg aagcgtatgc ccggcgggtt tgtcgcaacg gggaccgagg caacggatat
    12361 caccgtgttc aaccagggct tcggcctgct gaccggtggc atagtgggta tgcaggatgc
    12421 gaccgttcca ggtcggctcc ccgtgcaggt ggcagtaaac ggtacggaac accccgtcgc
    12481 ggcaaaaaac ggaaatcgtg ctgctgactg tcatgaaaac gtctccttct gctgaatcag
    12541 gtcgccgggc cgaccggttg ctccccggag gggcgaaagc cccttctgcc gtccacttct
    12601 tcggggtttc tccccgcagt ggacctttcc gatggggcgt ccgtcaccac gtcacggggc
    12661 agctgtccgg gggcggacgc aaaatttttt gccgggttta tggctaaaaa ttttgtggac
    12721 ggtatatctt cccctggact gctgaaacgg gatgtggtta ctccagcccc cggaaagtcc
    12781 acagcgggaa acaggccgca ggggtggcgg cggaagggtt gactgcgcca tcaggcgcag
    12841 tggccttaca caccgtcagg tgtgcaggga gcgccgtttc cggcgcgaca ggggcggcgc
    12901 gaccggaacg gtcgctgcaa gcctggtttt tcagacatcg gaccgtagcg gcagcccgaa
    12961 accgcgccag cgggttcggc ggagactggc gggtgcgcca gcagctgcct gtcggagtcg
    13021 ggcctgacga ccggatgccg ccaggcagcc gggagccgaa ggtgtccccg cctccggaaa
    13081 tcagaacaac agaaacgaca taacagaaaa atgcctcaca ccatgacggg caggaagggc
    13141 agcgccgcag gcgcggccct cctgcatttc aggcaggcgt aagcctgcca gggttttccg
    13201 gcggcctggc aggccatacc cccgccggag cgggggagta aatcaccgac ggaacacatg
    13261 accgtcaata aaggtgaagg agtccaggaa taaatcccgc gcatacgcct catagtcaaa
    13321 atagagcgcc accgtctccg gcacgtcagc cagcaggccg gtatcactga cgaactcaac
    13381 cgcaaaagcc tcctcactgt cagcctcgcc ccaccaggca tcgcggaagg tgtcaaaatc
    13441 cgtctcaccg gtatcatcca cccagagacg ataagcctct tcgcagcctt catcccgtgc
    13501 ccggcggaag ccttcaaccc aggcccagtt gatatggcat tcagaggcca tattccccgg
    13561 gaatccctca taatcctgaa acatcagttc aggatcggct tcatcctggt gaagagcacg
    13621 gcaggcggcg aaaaagtcgc gctcatcatc aaacgtggtc aggtcaaacc agcgtccggc
    13681 gatgcttcca cagttgtatt tgtgccaggt tccaacgtat acagcaggtg caacaacaga
    13741 cattcccgaa tcctccatag tcacatgttc tgcg
//
LOCUS       DAZOXR010000112        13592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZOXR010000112 DAZOXR010000000
VERSION     DAZOXR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.114"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(36..119)
                     /locus_tag="ACHUNN_002826"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="ACHUNN_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUNN_002827"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUNN_002827"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR0577150.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUNN_002828"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUNN_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR0577151.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUNN_002829"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUNN_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0577152.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUNN_002830"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUNN_002830"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0577153.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUNN_002831"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUNN_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0577154.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="ACHUNN_002832"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="ACHUNN_002832"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0577155.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUNN_002833"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUNN_002833"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0577156.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="ACHUNN_002834"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="ACHUNN_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUNN_002835"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUNN_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0577157.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="ACHUNN_002836"
     CDS             complement(12425..12664)
                     /locus_tag="ACHUNN_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0577158.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUNN_002837"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUNN_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0577159.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="ACHUNN_002838"
     CDS             13041..13328
                     /locus_tag="ACHUNN_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0577160.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3543 a         3333 c         3484 g         3232 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    13501 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    13561 tttctctctt gatttttttg ctcattcatc aa
//
LOCUS       DAZOXR010000113        13512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZOXR010000113 DAZOXR010000000
VERSION     DAZOXR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.115"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            7..1773
                     /gene="adeD"
                     /locus_tag="ACHUNN_002839"
     CDS             7..1773
                     /gene="adeD"
                     /locus_tag="ACHUNN_002839"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0577161.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(1818..3209)
                     /gene="uhpT"
                     /locus_tag="ACHUNN_002840"
     CDS             complement(1818..3209)
                     /gene="uhpT"
                     /locus_tag="ACHUNN_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0577162.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3347..4666)
                     /gene="uhpC"
                     /locus_tag="ACHUNN_002841"
     CDS             complement(3347..4666)
                     /gene="uhpC"
                     /locus_tag="ACHUNN_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0577163.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4676..6178)
                     /gene="uhpB"
                     /locus_tag="ACHUNN_002842"
     CDS             complement(4676..6178)
                     /gene="uhpB"
                     /locus_tag="ACHUNN_002842"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0577164.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6178..6768)
                     /gene="uhpA"
                     /locus_tag="ACHUNN_002843"
     CDS             complement(6178..6768)
                     /gene="uhpA"
                     /locus_tag="ACHUNN_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0577165.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6843..7133)
                     /gene="ilvN"
                     /locus_tag="ACHUNN_002844"
     CDS             complement(6843..7133)
                     /gene="ilvN"
                     /locus_tag="ACHUNN_002844"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0577166.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7137..8825)
                     /gene="ilvB"
                     /locus_tag="ACHUNN_002845"
     CDS             complement(7137..8825)
                     /gene="ilvB"
                     /locus_tag="ACHUNN_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0577167.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8931..9029)
                     /gene="ivbL"
                     /locus_tag="ACHUNN_002846"
     CDS             complement(8931..9029)
                     /gene="ivbL"
                     /locus_tag="ACHUNN_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0577168.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9594..9683
                     /gene="tisB"
                     /locus_tag="ACHUNN_002847"
     CDS             9594..9683
                     /gene="tisB"
                     /locus_tag="ACHUNN_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0577169.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9807..9881)
                     /gene="ysdE"
                     /locus_tag="ACHUNN_002848"
     CDS             complement(9807..9881)
                     /gene="ysdE"
                     /locus_tag="ACHUNN_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0577170.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9963..11147
                     /gene="emrD"
                     /locus_tag="ACHUNN_002849"
     CDS             9963..11147
                     /gene="emrD"
                     /locus_tag="ACHUNN_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0577171.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11155..11652)
                     /locus_tag="ACHUNN_002850"
     CDS             complement(11155..11652)
                     /locus_tag="ACHUNN_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0577172.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11649..12011)
                     /locus_tag="ACHUNN_002851"
     CDS             complement(11649..12011)
                     /locus_tag="ACHUNN_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0577173.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12001..12348)
                     /locus_tag="ACHUNN_002852"
     CDS             complement(12001..12348)
                     /locus_tag="ACHUNN_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0577174.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12457..12906
                     /gene="yidI"
                     /locus_tag="ACHUNN_002853"
     CDS             12457..12906
                     /gene="yidI"
                     /locus_tag="ACHUNN_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0577175.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12953..13111)
                     /locus_tag="ACHUNN_002854"
                     /pseudo
     CDS             complement(12953..13111)
                     /locus_tag="ACHUNN_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13155..>13512)
                     /locus_tag="ACHUNN_002855"
     CDS             complement(13155..>13512)
                     /locus_tag="ACHUNN_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0577176.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3186 a         3775 c         3533 g         3018 t
ORIGIN      
        1 aaaaatatga ataattctat taaccataaa tttcatcaca ttagccgggc tgaataccag
       61 gaattgttag ccgtttcccg tggcgacgct gttgccgatt atattattga taatgtctct
      121 attctcgacc tgatcaatgg cggagaaatt tccggcccaa ttgtgattaa aggacgttac
      181 attgccggtg ttggcgcaga atacgctgat gctccggctt tgcagcggat tgatgctcgc
      241 ggcgcaacgg cggtgccagg gtttattgat gctcacctgc atattgaatc cagcatgatg
      301 acgccggtca cttttgaaac cgctaccctg ccgcgcggcc tgacgaccgt tatttgcgac
      361 cctcatgaaa tcgtcaacgt gatgggagaa gccggattcg cctggtttgc ccgctgtgcc
      421 gaacaggcaa ggcaaaacca gtacttacag gtcagctctt gcgtacccgc cctggaaggc
      481 tgcgatgtta acggtgccag ttttaccctt gaacagatgc tcgcctggcg ggaccatccg
      541 caggttaccg gccttgcaga aatgatggac taccctggcg taattagcgg gcagaatgcg
      601 ctgctcgata aactggatgc atttcgccac ctgacgctgg acggtcactg cccgggtttg
      661 gggggtaaag aacttaacgc ctatattact gcgggtattg aaaactgcca cgaaagttat
      721 cagctggaag aaggacgccg gaaattacaa ctcggcatgt cgttgatgat ccgcgaaggg
      781 tccgctgccc gcaatctcaa cgcgctggca ccgttgatca acgaatttaa cagcccgcaa
      841 tgcatgctct gtaccgatga ccgtaacccg tgggagatcg cccatgaagg acacatcgat
      901 gccttaattc gccgcctgat cgaacaacac aatgtgccgc tgcatgtggc atatcgcgtc
      961 gccagctggt cgacggcgcg ccactttggt ctgaatcacc tcggcttact ggcacccggc
     1021 aagcaggccg atatcgtcct gttgagcgat gcgcgtaagg tcacggtgca gcaggtactg
     1081 gtgaaaggcg agccgattga tgcgcaaacc ttacaggcgg aagagtcggc gagactggca
     1141 caatccgctc cgccatatgg caacaccatt gcccgccagc cagtttccgc cagcgacttt
     1201 gccctgcaat ttacgcccgg aaaacgctat cgggtcattg acgtcatcca taacgaattg
     1261 attacgcact cccactccag cgtctacagc gaaaatggtt ttgatcgcga tgatgtgtgc
     1321 tttattgccg tacttgagcg ttacgggcaa cggctggctc cggcttgtgg tttgcttggc
     1381 ggctttggac tgaatgaagg tgcgctggct gcgacggtca gccatgacag ccataatatt
     1441 gtggtgatcg gtcgcagtgc cgaagagatg gcgctggcgg tcaatcaggt gattcaggat
     1501 ggcggcgggc tgtgcgtggt acgtaacggc caggtccaaa gtcatctgcc gttgcccatt
     1561 gccggcctga tgagcaccga cacggcgcag tcgctggcgg aacaaattga cgccttgaaa
     1621 gccgccgccc gtgaatgcgg tccgttaccc gatgagccgt ttattcagat ggcgtttctt
     1681 tctctgccag tgatccccgc gctaaaacta accagtcagg ggctatttga tgtcgagaag
     1741 tttgccttca ctacgctgga agtcacggaa taataaaaaa gcccggcgtc atgccgggca
     1801 aaagtcacca gttacgttta tgccactgtc aactgctgaa tttttttctc gcggcggatt
     1861 ttgcgttctt ccatcaccgc cactatcgcc atcaggcaga tacaaccaat cgcggcgata
     1921 tcaagtgcgg cgaaggtgcc tgcccagccg gtaaggccga ataccggcgt cccatcggca
     1981 atcattccca gacctaactt ggcaaagctg tcaccaatca ggtaagcaaa ggtgccttta
     2041 ataccatcgg cagcgccaat cgctttttta ggtacaaagc caacagcagc cacaccaatc
     2101 aacaattgcg ggccaaagac caggaaaccc aacgcaaaga gagaagccag atagatatat
     2161 tcgttactgg catgttgata cacaccgagc gtggcgataa tcagcgccag cgcgatgcag
     2221 gccaccaggc cacggcgacc gttcgccagg tcagagagcc agccccacag cagcgtaccg
     2281 accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg ccaccgcttt agagagtttc
     2341 agttcctgga acgcgtatac ggttgaccac tggtcgatac caatacgtac cacatagagg
     2401 aaaatgttgg cgaagcacag cagccagatc actttgtttt tcagcacata ctcaacaaag
     2461 atctgccact tggtcatatc ggtagattct gtctctttgt cctcttcgct gatctcctcg
     2521 ccgaacagtt cttcagcttt gccgaggcca taagattccg gggagtcgct gccgtaacgc
     2581 aggccgataa aaccgacaat cagcgcgata atcgacggga agataaacat gccgatgaca
     2641 tggccatcga acaggtaatt tgccccgaac agcgccacgc ctgctgcgcc tgcaccgcca
     2701 aggttgtgag aaatattcca gaaaccgagg aatgtcccgc gtttacgacg cggcgtccat
     2761 ttggtgatgg tggagtaact gcacgaaccg ccggtactct ggaaaaagcc gcttaaggcg
     2821 tagaaggcaa tcatcaggaa caggctaacc gagccgctgc ccatactggc actgaagccc
     2881 agcatacaaa tggcagacag gatcagcatg aacggcagga attgtttggt gtttttgccg
     2941 tcggcgtagt aggaaaccag cgttttaccc acgccataag tgatggagaa acccaggccg
     3001 atcatcccca gctgcgtcat gctcaacccg taggtcgaga tcatgtcgtt ctgcgcgatg
     3061 ttaaagttct tgcgaatcag gtacatcgtc aggtagccga taaagaccac caggtaggat
     3121 tgcatgaacg gtttgaacca cattttgcgc cgcacttcga gcggaaggtc cagggtcggc
     3181 ttgcgaacct ggtttaagaa agccagcatg ggttactcct gaaatgaata cctgcgtgag
     3241 gcatgcattg taaaaatcag cagcaaatca ggcctgagat agcgtccagg caaaacctgg
     3301 aaaatttctt agttttgccg gatatgaagt gaaaaggtga gatgcatcac gcttcgcgcg
     3361 gtgtctgggc gttcaaaaag ggcagtaaca gcagtgcgga aatcccggcg gcgatagaga
     3421 taaccacaaa aaatccgctc cagtgccagg tatcgagtac tttcgccagc ggccaaccag
     3481 caagcgacgc ccccagataa gcaaacaagc cgacaaaccc cgtcgccgcc cctgccgcct
     3541 ctttgtggga acactctgcc gccgccatac cgattaacat ctgtgggcca aagacaaaaa
     3601 aaccaatggt gaagaagcag gttgcctgca tcacgtagct ggcaaatggc atcagccaca
     3661 gggagccgac tgaaagcaaa attccggcgg cgaaaatcaa attcatcggc cctcggttgc
     3721 cgttaaacaa tttgtccgag ccccaacccg ctaccagcgc accgataaat ccacccagtt
     3781 caaacatcgt cactgccgta ttcgccgtga ccagatcgac gcccagcgtc tcggacatat
     3841 acaaattgcc ccagtcgttg atcgccgccc ggaccacata gaccagcaca tagcaaaacg
     3901 aaagcagcca gatatacgga ttcagcaaca catatttggt gaggatctct ttacgcgtca
     3961 gccctgcccc ttcttgttgt tgagctatct ccagcgcgtc gtgtcgccat tcaccgaccg
     4021 ccggtaaacc taacgcctgc gggcgatcgc gcagccgcca gcagagaaaa attcccacga
     4081 cgatcgccat acaaccagca atcatcatcc cggcacgcca gccgtaatgt agtgcagccg
     4141 ctgccatcac aatgggaatg agtgcgccgc cgacgttatg cgccgtgttc cataatgccc
     4201 accaaccacc gcgctcggta cgtgaatacc aggccgttaa cagacgcgca cacaccggtg
     4261 aaccccagcc ctggaaaaag gcgttcagca cccagagtac ggcaaacgcc catagcgacg
     4321 tcgagaagcc aaacagaatg ttgataatgc ccgtggcgat aagccctatc cccataaaat
     4381 aacgggcatt tgagcgatcg ctgacaatgc cggagacaaa cttcgacacg ccataggtaa
     4441 tgtaaaacag ggtcgctaac aggccgatat cgctacggct gagcacgccg ttagcaagga
     4501 tttctggtac ggcggcgtta aaacttttcc gcgtgaagta aaacagcgcg taacccagcc
     4561 agatggtcag cagaatatgc cgacgccagt agcgatagcg ggcatcaatt tcatatttat
     4621 cagccattaa tggcgcatcg gcaggcgctt tcagaaacgg caacatcatc aaaccttaga
     4681 catagcgttg aggtagagaa acgctgacac gcgtgccgtg cagacaggaa atgtgtaatg
     4741 tgccacccag cgccgttacg cgctcgcgca ttccggtgag gccaaaacct tgttgcccgg
     4801 aacccggcgg caaaccgctg ccatcgtctt caataaccag catcaaccgt tcatcctgct
     4861 gccagccttg cagggtgacc gcgctggcat cagcatgttt cacaatgttg ttcagccctt
     4921 cctggcagac acgaaacagc gtcacgcgct ggttttcgct taacgctgat tcatcgattc
     4981 gccattcgag atggctgaca ataccgcgcc cttccagctc catttcccgc atcagtgagc
     5041 ggatggcctg ctccagggtg agatcatcca actggcgcgg acgtaaccga cccaacaaac
     5101 ggcgcaccgc gtcgtaaacg cccagcgata gttgttcgat gagttgcccg ctctgcttca
     5161 cgctggcgtt atctgccgcc agccgctgaa caatgcccgc ctgagtacga atagcagtga
     5221 tggtctggcc gatatcatca tgcagctcac gcgccacatc acggcgcacg ctctcttcgg
     5281 tttccagcaa ccgttcagcc agatgctgat tgcgcgccag ttccttttgc agcgactggt
     5341 taagttcacg caaccgctgg atgccagcgc caagcaacaa ccctgtcaga ctttgcacca
     5401 gcagcgagag caataaatcc accggatgat cgcgccaggt ttgactggcg atcagcgcga
     5461 tggcgttcat caacgtcgca atcagcgccc cttgccaacc atagtgccag gccagcgcga
     5521 taatcggcag caccagacag aatggcgtaa agcgcgacag ttcgtccggc aatcccaact
     5581 ggagccagag actgataaca aacagcagca agtaccagac cagatgtcgc ccgcgccagt
     5641 tgattggctg agaaaccagt gacgggccga gcggcagcca ggtgttattg gcgagatagt
     5701 gccagaacac cagacatatc ggggccagcg tcaggccgcc agttaaagtc agcaacagcg
     5761 cattccacga ctctttgccg tgccaaagcc agggcagcga ctgcaacaac gccgccgccg
     5821 ttaacgccgc cccctgtaac agcaaggtgc gccagtcacg ctgatggcga tagcgcgaga
     5881 tcagcgctac gggcagtaac gtcagtaaac taccgatcat caataacgga aaatgggtta
     5941 aaccgaccgc ctgcgttagc cagtaaatca gcagccactc cgcacccagc aatacgggcc
     6001 agtatccgcg cgggcattgc agcattagcc ccagacgcag accaaacgga aataacagca
     6061 ccgccatatc agggcgctca accagatgca ggctgatact ccacaggcaa aaccatgcgg
     6121 cagagaagat aaaaaagcag gcaataacgg taattaagcg ggagaacaac gtcttcatca
     6181 ccagccatca aacatgcggc gcgccagctc tacgtcgtta ctgacgccca gtttttccat
     6241 cagattggcg cgatggacgt gtaccgtttt cggtgacaag cccagttcgg cggcaatctc
     6301 tttcaccgcc attccttgcg ccagtttttc cgccacctgg cgttcacgtt tggttagcgg
     6361 gtcctgacga ccggatgcca gtttaatggc aatatccggc gtcagataac agccacccgt
     6421 ggcaaccgta tgcaccgcag caatgagttc atccgggcta cagcgtttgg aaagaaagcc
     6481 gcgtgccccc gcgttaagcg cctgctcaac cagcgcagga ctgtcgtgaa cggagagcat
     6541 aatcgtcgcc atacctttcg gcagctggct tagcagctcc agaccggaga tatcgggcat
     6601 ggagatatcg caaatacaca cctgcacacc gcgccccggc agccccgcca gcgcctcgcg
     6661 ccccgaacca aactcggcaa ctacctgcaa atcaggttcc agccccagca gctgcgcaaa
     6721 gccggagcgg acgatgaggt gatcgtctat aagggcaacg gtgatcatgg tcttgtcctg
     6781 gcgggtaaaa aaaaacgcgc ttaccttaac gataagcgcg atgttgttca agccttgagc
     6841 ggttactgaa aaaacaccgc gatcttgtta aacatcgtcg gatcggactg attacgctgc
     6901 actttcacga catcttccag cttatcgatt tggcttatca tctgctccag acgctggtcg
     6961 tcattgacca gtagccagat atggcttttg tcgctgtcct gaatcggcag acaaagaatg
     7021 ccttcaacgt taaaagcgcg gcgggcaaaa aggccacaaa cgtgggtcat tacgcccgga
     7081 tggttgcgaa cggtgagctc cagaattacg ttgtcatgag ttgtgttttg catggcttat
     7141 tcccccacca tttcagtatt cgccgcacct ggcggcacca tcgggtaaac tttttcttcg
     7201 gcatcaatgc gcacatggat cagcgccggg ctagggcgat tgatgatttc ctgcaatgca
     7261 gcctgcggat cggcttcgtt attcaaatca caggtttcga ggccgaatcc ggcggcaatc
     7321 tgcataaagt tgattttgcc cggataggtg gcggcaaaaa cgccttgctc gtagaacaga
     7381 ctctgttgct gatgcaccag ccccagcgct tcgttgttca tcagaatgat tttgacatcc
     7441 agctgatttt cactggcggt cgccatctcc tgaatattca tcatcaggct gccgtcgccg
     7501 gagaaacaca acactttgcg atccgggttc gccagcgccg cgccaatcgc cgcaggcagg
     7561 ccaaaaccca tcgtgcccag cccaccggag gtcagccact ggcgtgggcg attgagcgga
     7621 taagcttgcg cggtccacat ctgatgctga cccacatcgg tggtgataat cgcattgtca
     7681 tcgacacagg cggcaacggc gttgatcagg ccgtaatggc ttaatggatc gcacgctttc
     7741 gggattggac acgggaattc gcgctgcaaa tccgctacca actggtgcca ctctgcacgc
     7801 ggttgcgctt ccacctgcgg gatcaattgc gccagcacgt catcaacatc cgcctgaatc
     7861 gccacatgcg gctgcttgat tttacccagc tctgcgcgat cgatatcgac atgaataatt
     7921 ttggcattcg gacagaactg ctcggttttg ccaatcgccc ggtcatcaaa acgcgcaccg
     7981 agcacaatca acaaatccgc ctcctgcaaa atatagttgg tactgcgcac gccgtgcatc
     8041 cccagcatac ccagcgacaa cggatgcgct tttggcagca tgcccagcgc cattaaagtc
     8101 atggtggtag gcagttgcgc tttctccgcc agttcacgca cccgcgcagg cgcattgatc
     8161 acaccaccac ccagataaag caccgggcgt ttggcagcgt taatcattgt agctacgtca
     8221 cgaatgcttt cttcgctaaa ggcgggtgca gcggcttttt ctgccaccgc gggctgagct
     8281 tcaatctcaa aaaccgccgt ttgtacatcc ttaggaatgt ctatccacac cgggccaggg
     8341 cggcctgatt gcgcaatgcg gaaggcatcg ctcatgacct gcgggagttc ttcgatatgt
     8401 ctgaccagat agttgtgttt ggtgatgggg atagagatgc cgtaggtgtc gacttcctgg
     8461 aaggcgtcgg tgccgatcat cgaggcggga acctgaccag tgatgcaaat cagcgggatg
     8521 gagtccagcc gcgcatcggc aatggcggtc accaggttag tcgcacccgg tccgctacag
     8581 gccatacaga ccgccggttt accgtcggtg cgcgccattc cctgagcgat aaagcccgcg
     8641 ccctgttcat gacgggccag aatatggcgg atttgcgtgc tttggcttaa ggcatcgtaa
     8701 acaggcagga tagaaccgcc cggaatgcct gtcacaatct taatgccctg ctgttccagg
     8761 aaatgaacga taaattctgc gccggtaaag cgcttacgcg tcaatgttgt gcccgaactt
     8821 gccatgctcc agtccttttc ttctgggccg actttccggg aggtgcttaa acgaaaaacc
     8881 ccgcccggtt tgcgccgacg gggttttgga atcgtgtgtt gttccagtcc ctacggcgca
     8941 ttgccgacga ccaccaccac acgcacgacg accactgcgg cggatggcgc agttggtagt
     9001 agttttgcgt tgaacatgga agtagtcatt ggggaccttg ttggttttgt gtttaacaat
     9061 atttatacaa gcacagcttt acaggggaga caatggaaaa tttttcagca agggaaaatt
     9121 gaggggttga tcacgttttg tactgaattg cagataacaa aaaaccccgc cggagcgagg
     9181 tttcgtcagt cgcccgcggc tggtaaccgc aaagcacact gtattatgtc aacacagaaa
     9241 gtatacgtgt tccgcgcaga acgcgcaatt tcggcacgaa ttttgacgta tttagtgcat
     9301 agttgagtat cgatcacagt ttgcgttttg tccaaatatt gctgtttatt tatacagtaa
     9361 acttctataa tatcactgta cgcaatgtgt tatgcggggg ccgcatcgtt acccggcgca
     9421 ctaagtcctg gctgaaacgg gtggtgccgt cagcgcctta accccgcgtg agcacactgt
     9481 gttatgtcaa caagcacaaa gtttctcctt gagataccgc gtgcacaaca gctggcaaca
     9541 ggcagcggaa aggtacgtca gctggcagtg ctcctgaacc acaggagacg cgtatgagcc
     9601 tggtggatat cgccattctt atcctcaaac tcattgttgc agcactgcaa ctgcttgatg
     9661 ctgttctgaa atacctgaag taattcagat tcaagtcgca ccaaagggga gcgggtaacc
     9721 gctccccttt tatatttagc gtgcgggttg gtgtcggatg cgatgctgac gtatcttatc
     9781 cgccctacca tctctcccgg caacatttat tgccgctttt gtttacatat tctgccgcta
     9841 aacaactccc cattcctggc gtatatctgg ctaacattca tcaatgtgat agattcctct
     9901 cccgcattta tgggaatgcg tagtgactta ctctaattat ttttataaaa gcatccgtga
     9961 taatgaaaag gcatagaaac gtcaatttgt tattgatgtt ggtattactc gtggccgtcg
    10021 gtcagatggc gcaaaccatt tatattccag ctattgccga tatggcgcgc gatctcaacg
    10081 tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct gctgacttac ggtgtctcac
    10141 agctgtttta tggcccgatt tccgaccgtg tgggtcgccg accggtgatc ctcgtcggaa
    10201 tgtccatttt tatgctggca acgctggtcg cggtcacgac ctccagtttg acggtattga
    10261 ttgccgccag cgcgatgcag gggatgggca ccggcgttgg cggcgtaatg gcgcgtactt
    10321 tgccgcgaga tttatatgaa cggacacagt tgcgccatgc taacagcctg ttaaacatgg
    10381 ggattctcgt cagtccgttg ctcgcaccgc taatcggcgg tctgctggat acgatgtgga
    10441 actggcgcgc ctgttatctc tttttgttgg ttctttgtgc tggtgtgacc ttcagtatgg
    10501 cccgctggat gccggaaacg cgtccggtcg atgcaccgcg cacgcgcctg cttaccagtt
    10561 ataaaacgct tttcggtaac agcggtttta actgttattt gctgatgctg attggcggtc
    10621 tggccgggat tgccgccttt gaagcctgct ccggcgtgct gatgggcgcg gtgttagggc
    10681 tgagcagtat gacggtcagt attttgttta ttctgccgat tccagcagcg ttttttggcg
    10741 catggtttgc cggacgtccc aataaacgct tctccacgtt aatgtggcag tcggttatct
    10801 gctgcctgct ggctggcttg ctgatgtgga tccccgactg gtttggcgtg atgaatgtct
    10861 ggacgctgct cgttcccgcc gcgctgttct ttttcggtgc cgggatgctg tttcctctgg
    10921 cgaccagcgg cgcgatggag ccgttcccct tcctggcggg cacggctggc gcgctggtcg
    10981 gcggtctgca aaacattggt tccggcgtgc tggcgtcgct ctctgcgatg ttgccgcaaa
    11041 ccggtcaggg cagcctaggg ttgttgatga ccttaatggg attgttgatc gtgctgtgct
    11101 ggctaccgct ggcgacgcgg atgtcgcatc aggggcagcc cgtttaagcg cacgtcaccg
    11161 cagcatcgtc atcagctcca tgggagaacg atgctgcttt atcagatcac gcatcacccg
    11221 catatgcggt gcggagtaag aataaaacgc ctgatagccc gcacaaagca cgctgtctgg
    11281 cgtctcctgc cgagcgtgcg ggcacaggcg atgccagtcg cacgcctggc agtcacagcg
    11341 cggttgcggg ttttcgctgc cacaccattt ttgtaaggtg gcgtcaaaaa gctcaatcga
    11401 gatacgactg atatctgccc gcacccaaca ctcgaacagg gcaattaaaa atttccccca
    11461 cagctctgct gtcaccgaag cggccgtcag atcgccccgg tcatcaaatt caagataggg
    11521 aatgagctgt acggagcgga tgccccgctg tttgaaattg tgataaaaat cggcaggttg
    11581 cagagcatcc ttgtactcta caaccagttt atgcctgtcc tcttcagcat caatgacctg
    11641 acttcctgtc atgcgactct ctcaataaat acgataagtt gatgtatatg cgttacagca
    11701 aacagtattg cgagagataa caccgcgagg gagatcaaaa atttgtcacg gactacgtga
    11761 aattgggaaa aatcgctatt cgtgacatcc attaaattgc gattacgggt gtagtgccag
    11821 aggatcagcg ccacgatggc gagaatgcca atcgaaatcc agaataacat gcccgcctgg
    11881 tgccagttgt gtttgatagc caacgccatc aacgcgccgt agcccagcat ggtacgaaac
    11941 cacgccagtg atgtgcgctc cggctgcagt cctgggtcgg caatgcgtct ggctttgcgg
    12001 ctatccggca tacaacacca gtcccataac gatcaccgcc acgaccatca atattaagct
    12061 aatgattaat aagctgttgg tgtagggcag gtcctctttc agacgcatcg ctttttcatt
    12121 gcgtaaccag cgcaggtagc catacattgc taacccgccg gaaaacagac acaacaacaa
    12181 cgccagcagt tcacgaatga cgggcgtggc gaaatctggc gcaagttgat caagcccaac
    12241 ccccgccgcc agaaatccta gtgcggtacg gatccacgcc agaaaagtac gctcattagc
    12301 cagcgagaag cggtaatcgg gcgcttctcc aaggcgggaa attttcattg ttgctccttt
    12361 gcaatttcac taactctcta cctttagcgg ttttttttaa gaatttcgtc cgcccgtttc
    12421 ctgatatgct tttgccatcc gctacaagga ggtcagatgg gtatcatagc gcaaaataaa
    12481 atcagttcat tgggcatgct gttcggtgcc atcgctttaa tgatggggat tatccacttt
    12541 tcattcggcc ctttttccgc accaccgcca acgcttgaaa gcatcgtggc ggacaaaacg
    12601 gctgaaatta aaagaggtct tctcgccgga ataaaaggcg aaaaaattac caccgtggag
    12661 aaaaaagagg acgtagatgt cgataaaatc ctcgatcaaa gcggtatcgc actggccatt
    12721 gctgcgctac tttgcgcatt tgttggcggt atgcgtaagg aaaatcgctg gggcattcgc
    12781 ggcgcgctgg tttttggcgg cggaacgctg gctttccata ccttgctttt tggtatcggc
    12841 atcgtgtgca gcattttgct gatattcctt atcttttcct tcctgaccgg cggttcgctg
    12901 gtttaagcag cctgaataat gcccgccggg tgatcatcag cgggcattat cgtcagaact
    12961 tctgtttttt ctcttccacc ttcacccctt gtgtcggtag gccggtgtca tagtcacgta
    13021 ccaccggcga atagccatct tgtgggcgtg gacgaaatgc ccccatccag cgcggccgtg
    13081 catctttacg ccacggacgc aggctccatt gttgacatcc tccacgccct gaaggacgtg
    13141 gacccctgct acgttcaggc tgtcgcctga atcatttcgg tgggttcctg cttcaacgag
    13201 cggcctgact gcaccatctc tccacaggca agaacggcgt gccccgccgc taaaatgtta
    13261 cgagcgccat ttacatcggc gttcgctgta tatccacata cctggcatct gaatttactt
    13321 tgtgacaggc gattttcttt cgctgtatga ccacagcaca cgcaacgctg gcttgtgtac
    13381 gctggcggaa cagcaagcac ctgaccacca cgccagagct gcttgtacgc aagctggcgg
    13441 cgcatttcat accagccctg atccagtatc gaacggttta aacctgattt tgcccggaca
    13501 ttgcgccccg gc
//
LOCUS       DAZOXR010000114        13374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZOXR010000114 DAZOXR010000000
VERSION     DAZOXR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.116"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            488..1003
                     /locus_tag="ACHUNN_002856"
     CDS             488..1003
                     /locus_tag="ACHUNN_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0577177.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1221..1949
                     /gene="artP"
                     /locus_tag="ACHUNN_002857"
     CDS             1221..1949
                     /gene="artP"
                     /locus_tag="ACHUNN_002857"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0577178.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1967..2698
                     /gene="artI"
                     /locus_tag="ACHUNN_002858"
     CDS             1967..2698
                     /gene="artI"
                     /locus_tag="ACHUNN_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0577179.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2705..3421
                     /gene="artQ"
                     /locus_tag="ACHUNN_002859"
     CDS             2705..3421
                     /gene="artQ"
                     /locus_tag="ACHUNN_002859"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0577180.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3421..4089
                     /gene="artM"
                     /locus_tag="ACHUNN_002860"
     CDS             3421..4089
                     /gene="artM"
                     /locus_tag="ACHUNN_002860"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0577181.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4381..5112
                     /gene="artJ"
                     /locus_tag="ACHUNN_002861"
     CDS             4381..5112
                     /gene="artJ"
                     /locus_tag="ACHUNN_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0577182.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5287..6414)
                     /gene="rlmC"
                     /locus_tag="ACHUNN_002862"
     CDS             complement(5287..6414)
                     /gene="rlmC"
                     /locus_tag="ACHUNN_002862"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0577183.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6455..6943)
                     /locus_tag="ACHUNN_002863"
     CDS             complement(6455..6943)
                     /locus_tag="ACHUNN_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0577184.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(7003..7848)
                     /gene="potI"
                     /locus_tag="ACHUNN_002864"
     CDS             complement(7003..7848)
                     /gene="potI"
                     /locus_tag="ACHUNN_002864"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0577185.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7845..8798)
                     /gene="potH"
                     /locus_tag="ACHUNN_002865"
     CDS             complement(7845..8798)
                     /gene="potH"
                     /locus_tag="ACHUNN_002865"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0577186.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8808..9941)
                     /gene="potG"
                     /locus_tag="ACHUNN_002866"
     CDS             complement(8808..9941)
                     /gene="potG"
                     /locus_tag="ACHUNN_002866"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0577187.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10036..11148)
                     /gene="potF"
                     /locus_tag="ACHUNN_002867"
     CDS             complement(10036..11148)
                     /gene="potF"
                     /locus_tag="ACHUNN_002867"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0577188.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11499..11975)
                     /locus_tag="ACHUNN_002868"
     CDS             complement(11499..11975)
                     /locus_tag="ACHUNN_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0577189.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12063..12965)
                     /gene="rimK"
                     /locus_tag="ACHUNN_002869"
     CDS             complement(12063..12965)
                     /gene="rimK"
                     /locus_tag="ACHUNN_002869"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0577190.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13026..>13374)
                     /gene="nfsA"
                     /locus_tag="ACHUNN_002870"
     CDS             complement(13026..>13374)
                     /gene="nfsA"
                     /locus_tag="ACHUNN_002870"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0577191.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         3394 a         3553 c         3281 g         3146 t
ORIGIN      
        1 gtagcataaa taatattatc gagagtcaaa gctgggacta tcaaatttcg ctcattttca
       61 aaaatacaac gtaattttgg caatcatctt aatcgcagga tctcttatcg catgtgaaaa
      121 attttgtaat gattagacaa taaccgcgtt catttatcac gcgttataaa tgtgaaactg
      181 gcttcttgca gatgctctta acctctacag gaggatttat accaccattt cagaactatc
      241 atatttacct taaatgatca tttcagccat ataacacgct caacctcaca aatggtttac
      301 actgtctatt gtttgggtaa tttaagtaag tcagctacga gcaaccttga gtggatagcc
      361 agcaatggac tcaaacccaa tcaggtgttc agattgtttc agaattgtat taaaaacaat
      421 gtattaaata atctgatagc gtttggtcgt agtgtgacgc agacagcaag ctaataagga
      481 aatgattatg cgctaccgtt cactatctct acttattcca tgtgcactgc tgctgagtgc
      541 ctgtaccacc gtcacgccag cttataaaga taacggtccc cgcaccggtt cctgtgtcca
      601 gggggggcct gacagcgtgg ctcaacaatt ctatgactac cgcattcaac atcgcagcaa
      661 tgacattacc gccctgcgcc cctatttgag tgacaaactg gcaacccttc ttagcgatgc
      721 cagccgcgat aatagtcatc gtgaattgtt aagcagcgat ccgttctcca gtcgaaccac
      781 cttgccagat agcgcccacg ttgccagtgc atcgactatc cctaatcgtg atgcccgtaa
      841 cattccattg cgcgtagatc tcaaacaggg tgaccagggt tggcaggatg aagtgttaat
      901 gattcaggaa ggccagtgtt gggtcatcga cgatgtgcgc tacttaggcg gcagcgttca
      961 tgccacggcg ggtacgctcc gtcagtccat cgagaatcgc taacgccttg atcaaccaat
     1021 aaaccccgta aattgtccgg catttcgtga ggaatgccga catttatgct cgccgaccac
     1081 cgcccccgtt attttgtgct atgtttattg aataatgcgc tttgctttta acttttaaag
     1141 cagaaatatt gcataattat tctgtcaaag gtactatctt cgacctcaat agctatcaga
     1201 ctgccagtat acgagtgtca atgagtattc aattaaacgg cattaattgc ttctacggcg
     1261 cgcatcaggc gctgttcgat atcacgctgg attgcccaca gggcgaaacg ctggtgttac
     1321 ttggccccag cggcgcgggt aaaagctcgc tgctgcgtgt actcaatctg cttgagatgc
     1381 cacgctccgg tacgctcaac attgcaggca accatttcga tttcaccaaa acaccctctg
     1441 acaaagcgat tcgcgatttg cgtcgtaacg ttggcatggt gtttcagcaa tacaacctgt
     1501 ggccgcatct gaccgtgcag caaaacctga ttgaagcgcc ctgccgtgta ctggggttga
     1561 gtaaagatca ggcgctggcc cgtgcagaaa aactgctgga acgtctgcgt ctcaaacctt
     1621 atagcgatcg ttacccgctg catctttctg gtggtcagca gcagcgtgtt gctattgccc
     1681 gtgcgttgat gatggaaccg caggtactgc tgttcgatga accgaccgcc gcactggacc
     1741 cggaaattac ggcacaaatc gtcagcatca ttcgtgagct ggcagaaacg aatattaccc
     1801 aggtgatcgt cacccacgaa gttgaagtgg cgcgtaaaac cgccagccga gtggtgtata
     1861 tggaaaatgg tcatatcgta gaacaaggcg acgcgagctg ctttaccgag ccgcaaaccg
     1921 aagcatttaa aaactatctc tctcactaag attcgggata acaacaatga aaaaagttct
     1981 gattgccgcg ttaattgcag gttttagtct ttccgccaca gctgccgaaa ccattcgttt
     2041 tgctaccgaa gcatcctatc ctccgtttga atcgattgat gcaaacaacc agatcgttgg
     2101 ttttgacgtc gacctggcac aagcgctgtg taaagagatt gatgcaacct gcactttctc
     2161 taaccaggcg tttgacagcc tgatcccaag cctgaaattc cgtcgcgtag aagccgtgat
     2221 ggcgggcatg gatatcactc cggagcgtga aaagcaggtg ctgtttacca ccccgtacta
     2281 tgacaactct gccctgtttg tgggtcagca aggcaaatac accagtgttg atcagctgaa
     2341 aggcaaaaaa gtcggcgtac agaacgggac gacacaccag aaattcatta tggataagca
     2401 cccggaaatc actaccgttc cgtatgacag ctaccagaac gcaaaactgg atctgcaaaa
     2461 cgggcgtatc gacggcgtct tcggtgacac cgcagtggtc actgagtggc tgaaagataa
     2521 cccgaaactg gcggcggtgg gcgacaaagt gaccgataaa gattacttcg gcactggcct
     2581 cggcatcgcg gtacgtcagg gcaacactga gctgcagcag aaactcaaca ctgcgctgga
     2641 aaaagtgaag aaagatggca cttacgaaac catctacaac aaatggttcc agaagtaatt
     2701 cctgatgaat gaattttttc ctttagcaag cgccgccggg atgaccgtcg gccttgccgt
     2761 ttgtgcattg attgtcgggc tggcgctggc gatgttcttt gcggtatggg agtcggcaaa
     2821 atggcgtcct gtcgcgtggg caggttcagc gctggtaacc attctgcgtg gcctgccaga
     2881 aattctggtg gtgctgttta tctattttgg ctcctcgcag ctgctgctga cgctttcgga
     2941 tggcttcact atcaatcttg ggttcgtgca gatcccagtg cagatggaca ttgagaactt
     3001 cgacgttagc ccgttccttt gtggtgtcat cgctctgtca ctgctgtatg ccgcctatgc
     3061 ctcgcaaacg cttcggggcg cgttgaaagc ggtgccggtg ggtcagtggg agtctggtca
     3121 ggcgctgggg ctgtcgaaat cggctatctt tttccgtctg gtgatgccgc agatgtggcg
     3181 tcatgcgctg cctggcctcg gtaaccagtg gctggtgctg ctgaaagata ccgcgctggt
     3241 cagtttgatt agtgtgaatg atttaatgct gcaaacaaaa agcatcgcta ctcgtaccca
     3301 ggaaccattt acctggtaca ttgtggcggc ggcgatttac ctggtgatca ccctgctcag
     3361 tcagtacatt ctcaaacgca ttgacctgcg cgcgacacgt tttgagcgga ggcccagcta
     3421 atgtttgagt atttacccga actgatgaaa gggctacaca ccagcctgac gctaaccgtt
     3481 gcctcgctga ttgtggcact gattctggca ttgattttta ccatcatcct gacgctgaaa
     3541 acgccggtgc tggtgtggct ggtgcggggt tatatcacgc tgtttaccgg tacgccgctg
     3601 ctggtgcaga tcttcctgat ttattacggg ccgggccagt ttccgacgtt gcaggagtat
     3661 ccggcactgt ggcatttgtt gtcagaaccg tggttatgtg cgctgattgc gttatcgctg
     3721 aacagtgcgg cgtataccac gcagctgttt tacggtgcaa ttcgtgcgat cccagaaggt
     3781 cagtggcagt cctgtagcgc cctgggaatg agcaaaaaag atacgctggc gctcctgctg
     3841 ccgtatgcct ttaaacgctc gctctcttct tattccaacg aagtggtgct ggtgttcaaa
     3901 agtacctctc tggcttacac cattacgctg atggaagtga tgggatacag ccagttgttg
     3961 tacggacgca cctacgatgt aatggtgttc ggtgcggcag ggattattta cctggtcgtc
     4021 aacggcctgc tgacgctgat gatgcgtctg atcgagcgca aagcactggc attcgaacgg
     4081 cggaattaat gtagctgcgt acattcgccg ggggggtttg taggtctgat aagacgcgcc
     4141 agcgtcgcat cagacatcag cacggtgcct gtgccggatg cggcgtggac gccttatccg
     4201 gcctacatag tgcataaatt cttataatga agacggacaa ctcactaagt tatccgtctt
     4261 tttttatttc atttaaatta tttaatcata tttattgcat ataaattcac ttgatggcat
     4321 tgttatccca tgccgcagac acggccaaaa atcataagat tgacagacgg gagttccatc
     4381 atgaaaaagt tagttcttgc cgctttactt gcttccttta ctttcggtgc ttctgccgca
     4441 gagaaaatca attttggcgt ttcagccacc tatccaccct ttgaatctat aggtgctaat
     4501 aatgagattg tcggctttga tatcgatctg gcaaaagcct tgtgcaaaca aatgcaggca
     4561 gaatgtactt ttactaatca cgcgttcgac agcctgatcc cgtcactgaa attcagaaaa
     4621 tatgactccg taatctccgg tatggatatc acgcccgagc gtagcaagca ggtatcgttt
     4681 accacgccct actatgaaaa ctcagccgtc gtgattgcca aaaaagatac ctacaaaacg
     4741 tttgccgatc tgaaaggcaa acgtattggg atggaaaacg gcaccacgca ccagaaatat
     4801 attcaggatc agcaccccga agtgaaaact gtctcctatg acagttatca gaatgccttt
     4861 atcgatctga aaaatggtcg tattgatggg gtatttggtg acacagcggt ggtaaacgaa
     4921 tggctgaaaa ccaatccgca actgggcgtt gctactgaaa aagtgaccga tccgcaatac
     4981 ttcggcaccg gtctgggcat cgctgtacgt ccggataaca aagctctgct ggaaaaactg
     5041 aataacgcgc tggcagcaat taaagctgac ggtacatatc agaaaatcaa tgaccagtgg
     5101 ttcccgcagt aattgagact gatgacaaaa gtaagattgc ctgatgcgct gcgcttatct
     5161 ggcccacatc aactctgcaa tgtattgatt tagcatgatt ttgtaggccg gataaggcgt
     5221 acacgccgca tccggcatga acaaagcgca cctgatcagc aatctgaagc cgcacctgcg
     5281 gcttttttat tgcttcacca gcagcgtcat cacttcatag tgcgcggtgt gcgggaacat
     5341 atcaaaaagc tgtacccgtt cgatgcggta acctggcagt tcgcggatat ctttcgccat
     5401 ggtttgggcg ttacagctgg agtagataat aaaacgcggt gccatcgttg agagataatc
     5461 acacagcggt ttaccaatgc cgcggcgcgg cgggttaacc agcaccagct ccggcacatc
     5521 cccctgagcg gtggcaaact gagtggagtc cagcgcctga aattgcaaac gcgttaagcc
     5581 cagttcagcg gctgactgct ttgcacaggc aatggcctct gatgcaattt cgatcccggt
     5641 taactgcatg tcaggcgtcg cgcagtgtaa accaaagcca cccacaccgc agaacagatc
     5701 ccacatatgt ttaactggca gctgccgtac ccagtcgcgc gcggtagcgt acagctggct
     5761 ggcgaccgcc ggattagtct ggaagaaact ttgcggacgg atccacagcg gtacgtcatt
     5821 aaaacgctct gccagcgcct gttgttcagt caggtagatc tccgtctccc cttccataat
     5881 cgccatatgt accggctgaa tattgacggt aataactttc agttgcggta gttgttcctg
     5941 taaccacggc agcgccttac gcagttgtgc cagtttggtt tcagaacgca gtacaaagcg
     6001 cagcatcatg ccgccatcgc tctggctttc agtcagcaga atgtatttca gttcgccacg
     6061 cttacgcgcc acgttgtagg gcgttaaccc cgcacgggcg ataaacggtt ttagcgccgc
     6121 aaaaacgggc gcaaaagagg caggataaag cgggcagtca caaaggtctt ccggcgtgcc
     6181 atcgcgatgc agcataccga gcagtggttt ttcaacgcta ccactcacca ccattttggc
     6241 tttattacga aacccttgtt ccgggcctga caccggcgcg caccattcct caaccggaaa
     6301 atcggcgagc agatttttaa gatcggcggt tttagcggag agttgctctg gaatcggctg
     6361 cgttatccac tgacaggaac gacagcgacc cgcgtcgtaa agtgcgcact gcatacattg
     6421 accttcacat catcaggggc gacgattata cacattattg caactggaag aaccgccgac
     6481 tggtcgaggg aacgaacagc agcatcagga tgagcatatc cggcagcttc tgcagcatca
     6541 ggctatggaa gatttcacgt tttgattcac cgggaatgct gaacagctcc ggataaccgt
     6601 accccagcga agccgcccac aagtaacttg cggcggtgat ttgtgtcagc agatatagcc
     6661 agcgcgccca gcgacgccct ttcaccagtg aaaacgcaca ccagatctca atgaaaacca
     6721 gcaccagact gcttaaaaag accagcgtta aactccaggt ttgtacgctg cgatgaatga
     6781 actcgccaat accgcgcacg cccagcgtat tgaaaatcat cagcacgtcg aggccacgga
     6841 tcataataat ggcgagcgcc gccacctgca ccagcgcagg cacattcagg cgagcatgag
     6901 atgaagatgt tttcttaaaa aatcccaacg tttcgtcttc catgaaaaca atgccgcggc
     6961 atgcgcggca ttatgtagcc aggttggcaa attttagtgt cttcagccat gtcttgcacg
     7021 ctggatatcg cgtatccgct gtttttctgc gcgagccatc agataccagg cgataaatcc
     7081 gacaattccg accgcaccga gaattaacgt tgcaagggcg ttgatttccg gattcactcc
     7141 catccgcacg ctggaaaaga ccagcatcgg taacgtggtg gctcccggcc cggaaacaaa
     7201 gctggcgatc accagatcat caagcgacaa agtaaaagcc agtaaccagc cagaaatgat
     7261 cgcgggcatg atcatcggta gcgtaatgac gaaaaacact ttcagcggcg tcgcaccgag
     7321 atccatcgct gcttcttcta tcgagcgatc cagttcccgc aatcttgaag aaataacgac
     7381 cgccacataa gccgtacaaa aagtgacatg cgccagccag atggtgagca taccgcggtc
     7441 cgcaggccag ccaatggcat gagcaagcgc gacgaataat aacaacagcg acaagcccgt
     7501 gatgccatct ggcatcacca gcggcgcggt gatcataaag gcaaaaccat ttgatccgcg
     7561 aaacctgcca aaacgcacca gcaccaccgc cgcaatagtc ccgaggatcg ccgccgccgt
     7621 tgccgcacag gcagcaattg tcaggcttaa accaaccgca ctcatcatcg cgtcatcgcg
     7681 caataactca ccataccagc gcgttgacca gccggcccac accgtcacca gcttcgagct
     7741 gttaaacgaa tagataacca gcatcagcat tggcgcgtag agaaaggtaa agcccagcag
     7801 caaaatcaca atccgccagg gcgaacgaac taccggtaaa ttattcatcc gtgttctccc
     7861 acgctttttt gctggtgttt gtggaaccac attatcggca caattagcag cagcaacatg
     7921 atgatcgcta ccgccgaggc caccggccag tcgcggttat tgaaaaactc ttgccataac
     7981 acgcgcccga tcatgatgct gtccgggcca ccgagcagtt ccgggatcac aaactcgccc
     8041 acagccggga taaacaccag catcgatccg gcaataatcc cacctttagt cagcggcacg
     8101 atcacagtaa agaacgtttt cagcggtcgt gcaccgagat ccagcgctgc ttccaccagc
     8161 gaataatcaa tacgaatcaa cgcggtataa atcggcagta ccataaacgg cacgtaagcg
     8221 taaacaatgc cgatataaac ggccagattg gtatgcagaa tggtcagcgg ttgatcgata
     8281 acccccagcc acagcagaaa attattcagc acaccgttgt tttttaatat tcccatccag
     8341 gcataaacgc ggatcagaaa cgaggtccac gacggcagga tcaccagtag taataaaata
     8401 ttacgggtcg aaggcttact gtgcgccacc gcccacgcca gcggatagcc gatcagtaaa
     8461 cagcaaaatg tcgaaatcgc cgccacctgt aacgactgga gataagcatc aaaatagagc
     8521 ggatcgtcgg tcagttgcag aaaattaccg agattaagag tgatggaaag ttgcccgtca
     8581 gcccactcca tcagttcggt ataaggtgga atagcgcgcg ccatctctcc caggcttatt
     8641 ttaaagacaa tcagaaatgg cagcagaaac agcaagatca accagatata tagcaacgca
     8701 atgaccagtt tgcgcccatg cttcatttgc agctgcgaca accacagctt aaatccgccc
     8761 ggcggtttcg actgggcagc aggttcaagt gtactcatct ttgctcctta aaccgtcagc
     8821 accacacagc tgtccacttc ccagcacaaa cgcacttcgt cgccccaggt cggtaaccct
     8881 ttacgatgac gatgggcgtt ttgtagctgg gcgctgatca tctgcccact tttcagtcga
     8941 acgtgataca ccgaaagatc gccgagatag gcaatgtgta tcacctcccc caccgcgaag
     9001 ttacaaccat tggcgggcgg ctcttcgcaa agcatgattt tttccgggcg cagcgctacg
     9061 tgtaccggca cgttatcgac cactgaagca tccgcatcga ctttcagtgg atgcaccagc
     9121 cccggcgaat caagcaccag gccatcttcc tgacgctctt tgagtacacc ttcaaagaca
     9181 ttaaccgaac cgataaattc agcgctgtag cgggtagttg gatgctcgta gatctcttcc
     9241 ggctcgccaa tctggacaaa tttcccgcga ttcataatgg cgatacgccc cgccatggtc
     9301 atcgcctctt cctgatcgtg ggtgaccatc acacaagtca caccgacgcg ctccagaata
     9361 tccaccactt caagctgcat ccggtcacgc agctttttat ccagcgcgcc catcggctca
     9421 tcgagcagta atagtttcgg gcgtttggca aggcttcggg ccagggccac acgctgtcgc
     9481 tgaccaccgg aaagctgatg cggtttgcgt ttggcgaact cctgcatgtg caccaggccg
     9541 agcatctcat ggacccgact ggcaatttcc gctttcggta gtttgtcctg tttcaggcca
     9601 aaagcgatgt tctgttccac ggtcatatgc ggaaacagcg cgtaagactg aaacatcata
     9661 ttgatggggc gcaggtaagg cggcacctgt gacaaatcga cgccatcgag cattatctgc
     9721 ccggcagaag gttgttcaaa acctgccagc atacgcagca gcgtggactt gccacagccg
     9781 gatgcgccca gcagcgcgaa gatttcacct ttgtagatgg tcaggctgac atcatcgacc
     9841 gcatgctgac catcgtagga tttggtgagg ttgcggattt ctaatagcgg cgtcagcgcc
     9901 ttacgggttt tcgcctgcgg gcgagggata gcgtcattca cggctgctct ccggcattga
     9961 atgtgtgcaa gcgcaccatg atggtgcgaa ttgccggctg gtgtggtgcg cccgcccggc
    10021 atctaaggct gcggattatt ttccgctctt cactttggtc cacgcgcggg tgcgcacacg
    10081 gtcgattttc ggatcctgta ctttcagagt gaatagcttc gcacgaacgt ccgccggagg
    10141 ataaataccc gggttctcac ggacttccgc actcaccagc ggcgtggctg ctttattggc
    10201 gttggcatag aacacatggt cggaaatatg cgctactaca tccgggcgca gcaggtaatt
    10261 caggaactga taggcttcgt ctttgttttt ggcatccgca ggcatggcga atacatcaaa
    10321 gaacgccatc gccccttctt ttggaatcga gaacgagaca ttcactccat tcttcgcttc
    10381 cttcgcgcgg tttgacgcct gccagacatc acctgcccag ccgatagcga cgcaaatatc
    10441 gccgtttgcc aggtcgttaa tgtattgaga tgaatggaaa taacgaatgt tcgggcgcag
    10501 ctttaacagc agatcggttg ccggtccggt gtaatcatcc gctttagtgc tgttgggatc
    10561 tttgccgaga taattcaaca cggtagcaaa aacttcttct ggcgcatcca ggaaagagac
    10621 accgcagctt ttcagttttt ccagattttc aggtttgagg atcaagtccc agctatcgac
    10681 gggcgcgttt tcgcccagca cagctttaac tttatcaacg ttatagccaa tcccggtcgt
    10741 cgcccacata tagggcatag caaatttatt gtcgggatcg tgtttggcga ccagcttcag
    10801 cagctccggg tcgagattct tccactccgg caatttgctt ttgtccagcg gctggaaaac
    10861 tcccgcagtc aactggcgtt caagaaagct ggcagatgga accaccaagt caaagccggt
    10921 gctacccgcc attaatttgc cttccagtac ttcgttagag tcgaaaacat cgtagacgac
    10981 tttaataccg gtttcttttt caaaattggc caccgtgtcc ggggcgatat aatcagacca
    11041 gttataaatg tggagtgttt tttgttcagc cgcgagcgtg ccgacagaga cggccatcag
    11101 agcacccgca gccagacccg aaagccattt tttatttaag gcggtcatat ctctttcctt
    11161 ctgaaagttc gttaacaaac gaacaaaagt tgtcacacta aaagatatgc aataaacgtg
    11221 catatttttt atcggcgggg tgtaagaaaa gagaactctc tcccggccag cactgctcgc
    11281 tatataaaag catcgcaaga ataactttag ccagggtttg agactatagc tcggattaaa
    11341 aaacggtatt taacaaattt ttatgcgtat ttgctctatg tgataaagcc aaatcgacac
    11401 aagagaagtg aaataatctt taagaaaagg ttaaaaacag cagataattt tgcagcgcgc
    11461 agccactgca ccagcgactg cgtgctcgaa aggagttatc agtggaggaa atagttttcg
    11521 gtaacgttgt tttgcccttc atcgtcagtc ggtaacagca gttgatgggc attagcttca
    11581 agaatgacca tcgaaatctg ctcttcgctc tgacgcacga accatgcaaa ctgctcatag
    11641 gttacgccct gcataacgga taaagactgg caaaccacca gctttggcag attatcatcc
    11701 tgcatatcaa gaaatgcttt cacggtcagc gaactggcat tgatggcaga taaatccgcc
    11761 gccagcggca atacggctga aggtcggact tccgccatgg cagaaaacag gatcgtgtta
    11821 tcgatcagat cgattttggc atcaaagaca ccgtcgaaat tctgcatatg gggcagatgc
    11881 agagcctgac agttatcaca ttcaaaaaaa ctcatcccca ggtcatcgag ccattgacgc
    11941 agcgtatcca gaccaggaac gaccagcgat gtcataattt tgtacaacct ctttcgataa
    12001 aaagaccggc acagcttacg caaaaagcgc aggcaaaacc atgatcagta atgtgattgc
    12061 gactaaccac ccgttttcag gcaatattct gtcgtagcgt ggcgttcgat ccagcggatc
    12121 attttacccg cgatgtcgat accagtggtt ttttctattc cttccagccc cggcgacgca
    12181 ttcacttcca tcaccaacgg cccgcgatta gcacgcagaa tatccacacc agcaacgtcc
    12241 agcgccatcg ttcgcgcggc ttttatcgcg atttcacgct cctgtggtgt gatactggcg
    12301 acgcttgccg cgccgccacg atgcaaattg gaacgaaaat cgccctcttt cgcccgccgt
    12361 tcaatcgcag cgaccacttc atcgccaaca accagacagc ggatatcgca cccttgcgcc
    12421 tctttgatat attcctgcac cagaatatgc gcgttcagac cgcggaaagc gtcaatcacg
    12481 ctttccgccg cctgacgcgt ctccgccagc acgacgccaa ttccctgcgt gccttcaacc
    12541 aacttgacca ccagcggcgc accaccgacc atgtcgatta aatcgctggt atcatccggc
    12601 gaatgcgcaa tgcccgtgac aggcaggtcg atgccctgac gcgccagcag ttgcatggaa
    12661 cgcaatttgt cacgcgcccg ggcaatggcg accgactcat tgagcggata gctccccagc
    12721 atctcgaact ggcgcagtgc cgccgtccca taaaaggtaa tggcggtgcc aatacgcggg
    12781 atcactgcgt caaaatgggg taacttgcgg cctttgtagt gaatagaaga cgccgcagga
    12841 tttatgttca tgtagcaaga aagcggatca agaatttcaa ccaggtgacc gcgctgtata
    12901 gcggcttcac gcagccgctt acacgaatag agcgttccat cccgggacaa tatggcaatt
    12961 ttcaccctgc acctctctgt cagacctggt caaaagcccg cgtatcatac atcgacgtgg
    13021 cggttttagc gcgtcgccca accctgtttg tgcaaataat ccagaataaa tgggcggctt
    13081 tctttaatga ttgttcggcg gatatgatcg ctccaggtat cccggcgatt attgctgcca
    13141 cgggtgaggt aatattccgc cagttgctcg tcatactgcg ccagtgcgtc tttatccagc
    13201 ggttgatagc tgttttcatg caccaaaatg gaggccggta aacgcggctt gagatccgga
    13261 ttatccgcag gccagccaag gcacagccca aacagcggca gaacatgctg cggtaatttc
    13321 agcagtttcg tcaccgcttc aatattattg cgcaggccgc cgatatatac cccg
//
LOCUS       DAZOXR010000115        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZOXR010000115 DAZOXR010000000
VERSION     DAZOXR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.117"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHUNN_002871"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHUNN_002871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0577192.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUNN_002872"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUNN_002872"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0577193.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUNN_002873"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUNN_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0577194.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUNN_002874"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUNN_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0577195.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHUNN_002875"
     CDS             complement(4036..5568)
                     /locus_tag="ACHUNN_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0577196.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACHGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUNN_002876"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUNN_002876"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0577197.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUNN_002877"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUNN_002877"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0577198.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHUNN_002878"
     CDS             complement(8663..10399)
                     /locus_tag="ACHUNN_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0577199.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUNN_002879"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUNN_002879"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0577200.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUNN_002880"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUNN_002880"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0577201.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHUNN_002881"
     CDS             complement(12121..12855)
                     /locus_tag="ACHUNN_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0577202.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHUNN_002882"
     CDS             13056..>13260
                     /locus_tag="ACHUNN_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0577203.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3365 a         3370 c         3489 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatggcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZOXR010000116        13258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZOXR010000116 DAZOXR010000000
VERSION     DAZOXR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.118"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..571)
                     /locus_tag="ACHUNN_002883"
     CDS             complement(<1..571)
                     /locus_tag="ACHUNN_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFR0577204.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYA"
     gene            complement(763..1146)
                     /gene="traM"
                     /locus_tag="ACHUNN_002884"
     CDS             complement(763..1146)
                     /gene="traM"
                     /locus_tag="ACHUNN_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR0577205.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            1467..2069
                     /locus_tag="ACHUNN_002885"
     CDS             1467..2069
                     /locus_tag="ACHUNN_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR0577206.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(2366..3187)
                     /locus_tag="ACHUNN_002886"
     CDS             complement(2366..3187)
                     /locus_tag="ACHUNN_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR0577207.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(3298..3594)
                     /locus_tag="ACHUNN_002887"
     CDS             complement(3298..3594)
                     /locus_tag="ACHUNN_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577208.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            3894..4190
                     /locus_tag="ACHUNN_002888"
                     /pseudo
     CDS             3894..4190
                     /locus_tag="ACHUNN_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4509..4634)
                     /locus_tag="ACHUNN_002889"
     CDS             complement(4509..4634)
                     /locus_tag="ACHUNN_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR0577209.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(4576..4725)
                     /gene="mok"
                     /locus_tag="ACHUNN_002890"
                     /pseudo
     CDS             complement(4576..4725)
                     /gene="mok"
                     /locus_tag="ACHUNN_002890"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(4904..5666)
                     /locus_tag="ACHUNN_002891"
                     /pseudo
     CDS             complement(4904..5666)
                     /locus_tag="ACHUNN_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(5663..6097)
                     /gene="psiB"
                     /locus_tag="ACHUNN_002892"
     CDS             complement(5663..6097)
                     /gene="psiB"
                     /locus_tag="ACHUNN_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0577210.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(6152..8110)
                     /locus_tag="ACHUNN_002893"
     CDS             complement(6152..8110)
                     /locus_tag="ACHUNN_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0577211.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(8174..8407)
                     /locus_tag="ACHUNN_002894"
     CDS             complement(8174..8407)
                     /locus_tag="ACHUNN_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR0577212.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(8463..8984)
                     /locus_tag="ACHUNN_002895"
     CDS             complement(8463..8984)
                     /locus_tag="ACHUNN_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0577213.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            9283..9726
                     /locus_tag="ACHUNN_002896"
                     /pseudo
     CDS             9283..9726
                     /locus_tag="ACHUNN_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9812..10375)
                     /locus_tag="ACHUNN_002897"
     CDS             complement(9812..10375)
                     /locus_tag="ACHUNN_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0577214.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(10422..11783)
                     /locus_tag="ACHUNN_002898"
     CDS             complement(10422..11783)
                     /locus_tag="ACHUNN_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR0577215.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(11835..12065)
                     /locus_tag="ACHUNN_002899"
     CDS             complement(11835..12065)
                     /locus_tag="ACHUNN_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577216.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(12289..12408)
                     /locus_tag="ACHUNN_002900"
                     /pseudo
     CDS             complement(12289..12408)
                     /locus_tag="ACHUNN_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT         3048 a         3733 c         3327 g         3150 t
ORIGIN      
        1 tagcatatcg caaccactca tccctgtctg agaaaccata gtgtttttta aaattcataa
       61 atattttttt agattggtca acagagatat ccattctctg agaagaatac tcatgactat
      121 aacccaaaag atacggtatt atcttattaa agcgatattt gtcaggttca caaccaaact
      181 ccttgccctt tataaaaaag ccactaacta cgtatctttc aaagaatctc cagacaaaca
      241 aattcaagtt attcacttcc gtactttcaa ccaccacatc ccatgataca tcattcaata
      301 ttacaccatt aataatactc actccattcg aggacgagaa agcatcaact tcagcagtat
      361 gaaataaaat ttgcgtatca atatcaatac cagaaaacca tatttcagaa tcactattct
      421 gacactttat taagtgtaaa aaaacagagt ttgagtctat gaattttcct gactctgtgc
      481 ggacacagac tggatatttt gcggataaaa taatttccgg caggcctgat tgacgtatga
      541 gtgctctttc tctagtatcc atcgcacaca taggaacctc ctcaaaggat tctatggaca
      601 gtcgatgcag ggagggacaa gctccctgca tcggcgattt tcaagttcaa attttaacgt
      661 ggcattaacc acgtaattaa tatactaatt attctgatta ttgtcatcag tttgaaccat
      721 taaaaagtct gatatttgaa caaagtcaaa atttatttcc ctttattcat catcattttt
      781 tggaaagaac ctttccatct cagccgacac cttctctctg atatcttcaa ccatattggc
      841 atattcaaac tttggatttc ctgagacatg aggactgaga gactcaatac ccaaaatttt
      901 cgctactgat gactgtgttt ttacaacgca ttcaagaagt agtttattaa actcggcctg
      961 attaaatgca gactctttgc gctccatctg agcctcatat acacgaagac ccagttcaag
     1021 aagcattgaa gccgttgctg aaaaactgac atctttttct ctcgccccct cctgtcgacg
     1081 tttttcaata atcgcgttta ttttttcata agcatcattg ctgatataca gattcacctt
     1141 agccattaga tattaccttt tttaaacata aaacaaagtt aatttcgaaa cactagaatt
     1201 catagtgcaa acagggacgc accgctagca gcgcccctga cggtatccta taaaaaaaca
     1261 caccgcgccg ctagcagcac ccctaatata aaataatgtt ttttataaaa atagtcagta
     1321 ccacccctac aaagcggtgt cggcgcgttg ctgtagctgc gttaacgacg ctgctttaaa
     1381 aaaatcagat ttaaacaata taaatccaca aatacaactc aatgatatta aagataaatc
     1441 agcaaaaact tgtttttgcg tagtgtgtgg tgcttttggt ggtgagaacc accaacctgt
     1501 tgagcctttt tgtggagtgg gttaaattat ttacggataa agtcaccaga ggtggaaaaa
     1561 tgaaaaaatg gatgttagca atctgcctga tgtttataaa tgggatctgc gaagccgccg
     1621 attgctttga tcttgcaggt cgggattaca aaatagaccc ggatttactg agagcgatat
     1681 catggaaaga atcccgttac cgggttaatg ccatcggtat taatccggta acgggatatg
     1741 gcagcggact gatgcaggta gattcccagc attttaacga actggcccgc tatggaatta
     1801 agccggaaca tctgacaaca gatccctgta tgaacattta taccggtgct tattatctgg
     1861 caatagcctt taaaaaatgg ggcgtctcct gggaggccgt tggtgcatac aatgccggat
     1921 tcaggaagac cgaacgccag aaccagagac gtcttgccta cgcatcagag gtttaccgga
     1981 tttatacctg gataaagagc agtaaaggca tccgggttcc gaccacgaag aaatcacttt
     2041 cccaaattaa cagtgtgcag aaaaattaat attgtataca ttgtcaaata ccggttatgc
     2101 cgcgacagcg gcatggaagg ccacacacaa aaatatcata tgagtgataa tgctgactgg
     2161 ccgaagcccc gcaaagcggg atcccatctt atatatatcc ggtaactgcg cagcctcctg
     2221 ccagaccatt tttcgcgggc acaatattta cagactaccg aaaagccagt tgatgaaatt
     2281 gaggcatatc agacgacaca gcaatgccgc aggatgataa tataagtaaa gccccgaaaa
     2341 atttttcggg gctttactta tattatcatg acttccactc cctgaactgt tcggcaatca
     2401 tccataatgc tttgttgatt cggatatcac cgtcaatgcc tgtaatggct cttgttctgg
     2461 tatttttacc ggaggcactt cgccccgata atccaccttt tatcatattc tcctgaaccc
     2521 gctgccaggt tgtccagaga tcgttctgtt tatcctccca acggcgggga gtaattattt
     2581 gttctggcgt cactggcgtt ttattttcat cttcgtacct ggccatcaat gcagctctgc
     2641 caaataaatg ttgttcgtca ctgttaagat gtatctcttt cattgtctcc atattttcag
     2701 tgactttatc aaagacaccg agcacttcat acgctccctc aataacctgc ccgacaatat
     2761 cacctttatg tggaacgcgg atttcgccaa aattattccc acacaccagg ccatttgtgc
     2821 agacaaaacg aaaaattcct gggatcatct gataactgga tgaaccatca tgtgaattaa
     2881 gcaggataat ttcaggaact tctttcccgt taatatgccc ttccctgcga agacgtaaca
     2941 tatgtttact gtattcgcgg cgtcccaaat cacgaacccg actctgacag gcaaagaatg
     3001 gctggaaacc ttcatcacgt aacttattga tgatattgat ggttgggata tatgtataac
     3061 gctcactccg ggattcatgt ttatcaccag aaaagacaga tggaacaaac tgcattaatt
     3121 cgtcatccgt taacgggcgc tcacggcgaa tagaattata tcgaccaaaa cgggaagcta
     3181 atcgcataac agtaactcct ttatgaaggc ataaaattaa taaaagtttt ttcgcttaat
     3241 atcatccccc ctcggttatc gttaacaaaa agacaacaat tccgagggga aggaccatca
     3301 aatcaggtca gttcgggact gaccaacctg ccgagtgaaa agcagtaaca gcgcgatcaa
     3361 ctcatcagcg ttttttctga caagaggagt atgattctcc aggctatcca gtaagtcatg
     3421 cagctcatta ttgacctggt ggagcacctt aaagagtgca ccagaatccc ggcagctctg
     3481 atatgcaaga tttgcctgaa cagcacgatt ctgcatgatc ccgatttgtg cgattgcgtc
     3541 tttctgctga tcgcctgttg gctgataagt ggtgttaaaa gaaaatgcgc tcataattcc
     3601 tcctgtatct gatgttttca gcccccatca tctgttgagt ttcgggagcc gcggttttga
     3661 cgttttgcgg ggacctgcct tttgttccct ccggtattca ccatcacctg tttgagaacc
     3721 ttcgccgcaa ggggcatcac tgccacggcc tgacgcacct gtcctgggca gaacgcaaaa
     3781 ttttttaccg ggttttgggt aaaaaatttt gtgggctgtt catttaccct ggacagtcag
     3841 tgcggcaggt tgtggctgtc tatgcccccg gcgacagggg cttaaacagg tgaatggcac
     3901 cggacagaac aacgggcagg gacccgccgc aacgacgtga agcgcggttc gcgaaacggc
     3961 gttgctcctt acatcccggc gtcttttgcc gggatgccgg gagcgcaggc cacggcgcga
     4021 caagggtggc gcgaccgttc cggtcgctgc aaacccgggt ttttgcgtca gggatggaag
     4081 cccgccaggg cggagacagc ttcgctggct ccgttcacga cagcccgccc cgccaggggg
     4141 acgcccggaa aaggcataag caggagaaca acaaaacaga caataatcag ggcagcagta
     4201 atcagagtca gagcagtaat cagagtcaga gcaggaggaa aaagagaata atcaaaggga
     4261 aggtgatgtg atttctggta agtcataaac agagaaggcg ggggaaaaga acgggcgccg
     4321 caggcgcacg ttcatccccg ttcccggagg gaaacggcct tcctggcagt ctggttgttc
     4381 atgcccgtac aatgccagca aggagaaagg ggccaccggc gaaccagcag cccctttata
     4441 aaggcgcttc agtgcagtca gaccagcatc agccctgaaa aggcgggcct gcgcccgcct
     4501 ccaggttgct acttaccgga ttcgtaagcc atgaaagccg ccacctccct gtgtccgtct
     4561 ctgtaacgaa tctcgcacag cgattttcgt gtcagataag tgaatatcaa cagtgtgaga
     4621 cacacgatca acacacacca gacaagggaa cttcgtggta gtttcatggc cttcttctcc
     4681 ttgcgcaaag cgcggtaaga ggctatcctg atgtggacta gacataggga tgcctcgtgg
     4741 tggttaatga aaattaactt actacggggc tatcttcttt ctgccacaca acacggtaac
     4801 aaaccacctt cacgtcatga ggcagaaagc ctcaagcgcc gggcacatca tagcccatat
     4861 ccctgcacgc tgaccacact cacttttcct gaaaataacc cgctcattca ggctgtttac
     4921 gggtaatctg tgtgattgtt gccgcatcac gccgcctccc ggagtttgtt cggcaccatc
     4981 caccgctcca tctcacggac agcgtgaccg gcctctcttg ctgcaagact ggcttccgca
     5041 ataacctgcc agaacggcat atcctgccac tgccagcgtt ccattccggc cagtgacgga
     5101 aaacgctcac cccactgcca gaacagggtt tccaggtcat gctctgccac ccggtcactc
     5161 cagcgtgcat accagcttcc gactgtttcc ggcgaatgga atgccagttc gatttcagcc
     5221 tgagcgacca gcgcctggta cgcacgggct ttctgctctg cctccctcgc ctgctggcgg
     5281 ctgtacttct gcatgaccag cgcggattta tggcgactgc gttccctgac acgaaaccgc
     5341 accgacggga acaagcgttc tgctgcatcg cctggaagag gaaggaggca gacctcgccc
     5401 tgtgtttcaa tcagcacatc gatggcatac agccactgaa gacggctgcc gtgcagttct
     5461 tctgcggtca gggacggcat gaaaaatcgc aggtcagaca gcgatatcct gcggcttccg
     5521 ttcagacagc ggaaaaacgc ccccgcatac ggatattccg ccagcgtgtt tccctggtgg
     5581 cggcgttttt ctgtctccgc caccgcccgc catgcggcct gttgctctgc gctgagggga
     5641 accagtgcct gtgaacgtgc gctcatgcct gacctccttc cgccatcagg atatggatga
     5701 tgtcactgac gctgtacccc tgcgcgtcca gtgacgccgc cagtgccagt gaatggctga
     5761 cggcttccgg tgcaaaccgc cgctgtacct gaaccaccgc aaacggctca ccaccggcat
     5821 tcaccagaac cagcacccag acctgagaga catcgcccgg tgaacataac gccaggtgaa
     5881 aacgttcgtg ggcaggcgtg aaacggacct ggacggggaa aaatccgcca aactcgctgc
     5941 cgtactcgcc gttcatcgtc cagttgtccg gcgcatccag ttcccgcatc acggcgtgtg
     6001 tcagctcacg acgttcatca cttccggcag cccggatatc ttcatattcc tgtgcgctca
     6061 tggtctgtaa tacattcagg gtcagttcag ttttcatcac ggtatctccc ttgctgccgc
     6121 cccgtctccg gcggggcggt gtggttgttc atcaggcggc atcagccagg ttatcggtgc
     6181 gttcagtggt atctgtctgt ggatgtggtg cacacatcca gccaggcacc cagcggttgt
     6241 ctttcatatg gtgctctgca tgttcagccg catcaccttt cttcatcttc tccgcgtccc
     6301 gtgcggcacc ggacagccct gcctcattca gggctgcgat aatctgcggc tttttcaggt
     6361 gtccgaagaa gtttgctttt gtcggctgcc accagtcgcg catgtgaaag ccgatggcgc
     6421 tttccagcgt gtcaagcgga ctgcgtgacg tgtgaccaca ctcacgggtc tggacaccgt
     6481 taaggctgca tgcggtgcag aaactgagca gggataacag cacctcctga ctgagtgaca
     6541 ggaatgtcgt catgtcccgt gaccatccct ccggtaacag ggcagcaaga cgggcttttt
     6601 ctgccatcag cgccatgaat gcggcacctt ccttccccga tggcgcatcg ctggtcagcg
     6661 aataatgttt acattccagg ctgatttgtg ctggtttact gtacgctccg ctgccaaaca
     6721 cattcaggca gagcgtccat gccagcagtg tcagggattt gtccggttgc tgcatcagtg
     6781 ctgcctggac tgccagcgtg cgttcggaag acattttcgt cagcagtggc agactgattt
     6841 cctccactga cgctttctcc tgcacctgtt ccgtacggtc agcgtcgtca gccacgtcat
     6901 cctcactgcg caactgcaca ccacgctgga cacacacgtt gccataacgc caggacacca
     6961 ccactccacc tccggcacgc atctccggcg tccacgccct gaccttcgcc atgcagcgca
     7021 tcagcttcat ttctgcttcc agcaggtcag attcctcaca ctggttttcc agcgcgtcgt
     7081 aacgcgtcat cagttcgttc agacgttctt cttccgcctc cgtcagcacc gcttccggct
     7141 ccggcagaca gcggtatgtt ccggcatcct cacggcactc accgacaggc tccatgcgtc
     7201 cggcgcacca ttcccagcct tcggcttccc gaaggaactc tgcgacagcc tggagttttt
     7261 ccagcagggc ggcatcgagc gccacgcagt ccacatagcc accttcgtcg tcgctgaaca
     7321 aatcggtgcg cagttcgtct ggcgagaagg catcagcccc cacgaagcgg aatttactgt
     7381 tccccgccac cgccacttca ctttcggtca ccagacgacg aatggtctgt acttccggtt
     7441 taccgcccca tcccgactga caggcggctt caaacacctg cacctgacgc gcggtgtcgt
     7501 tctccagcgc cagcgcctga cagtgttcgg tggtgatgcg gtcttctgcc agcgcatcga
     7561 ggatgacagg cgcaaggtca gccagtttca gcattcgctg aacgtggcgg ggtgaatagc
     7621 ccagcaaatc accgatttgt gcaggtgttt tgccttcctg cgccattgcg cggaatccgg
     7681 caatctgttc ggcagggtgc atatcccgac gctgaccgtt ttcagtcatt gatgcagccg
     7741 tcgccagctc ctgcggaata accttcacgc gtacaggcca gtcagccgga aggatgccac
     7801 gctctgccag catgttgagt gctgccagtc ggcgaccacc tgcggcgaca ccgtggcggt
     7861 caccaggcag ggcatgaaca accagattct gcaacaggcc aacgccctta atggactccg
     7921 ccagttcgct gacggactct gcggaatacg gcaccgtgcg cacattcagc ggtgatttaa
     7981 tcagcgaggc cagcgacacg ctcacttcct ccgtctgctc cagcagggca gacaggactg
     8041 tttcctgcgt ttttgcaggt ttacggctgg atttacgctc cgtttttgtc ttagactctg
     8101 taactgacat atatttctcc tttgaccggg atggttgtat gtctgccgga cgcgttcccg
     8161 tccggcacct ctcttactta agaatgccga aattccggat gacatggttc atccagtacc
     8221 cggcaccgtc ctcaaaattc caggtacgcc agaccaggcc actgttattg cggacaacca
     8281 ggcgaaaatg cgttccctga tcatcctcga taaagacatt ccggtactgt gcggcaacgg
     8341 cttcagcctg ttcgcgggta aaggagccgt caggcagccc ctgaaatatt ctgaaatatt
     8401 cgctcatcct catatctccg gtgatgcccc gcactggacg gggcggttgt cactgtcttt
     8461 cgttagaacg gaatgtcgtc atcgaagtca tacgcgggtt cagacggctg ctgaggctcc
     8521 ggctgcgcgg ccttacgtcc acggcctttt gttttcgcgc tccctttttt cgtcgtgtca
     8581 gcactctgtg gctgaccatt ctgctgaggc tgcggttgcg cctgagcgtt ctgctgtggt
     8641 gcactcccca gcatctgcat ggtgcccgtg gttttcacaa ggatttcagt gacgtaacgg
     8701 gtaatacctt tatcttccca gctacgggtt cggagttgcc cctcgatgta gacctgcgcg
     8761 cccttgcgca gatattcacc tgccacttcc gcgagcttgc cgaacagcac cacgcgatgc
     8821 cattctgtct gctcccgcat ctcccccgtc tgtttgtcac gccagctttc tgacgtggcc
     8881 acctgcaggt ttgccactgc gcccccgttg gggatgtaac ggacttccgg atcttttccc
     8941 agacgaccga caagaatgac cttgttaatg ccacgaactg ccatgatgtg tctccttctg
     9001 ttgatgtttt cagcccccat catctgttga gtttcgggag ccgcggtttt gacgttttgc
     9061 ggggacctgc tctttgttcc ctccggtatt caccatcacc tgtttgagaa ccttcgccgc
     9121 atggggcatc actgccacgg cctgacgcac ctgtcctggg cagaacgcaa aattttttac
     9181 cgggttttgg gtaaaaaatt ttgtgggctg ttcatttacc ctggacaggt gcggcaggtt
     9241 gtggctgtct atgcccccgg cgacaggggc ttaaacaggt gaatggcacc ggacagaaca
     9301 acgggcaggg acccgccgca acgacgtgaa gcgcggtttg cgaaacggcg ttgctcctta
     9361 catcccggcg tcttttgccg ggatgccggg agcgcaggcc acggcgcgac aagggtggcg
     9421 cgaccgttcc ggtcgctgca aacccgggtt tttgcgtcag ggatggaagc ccgccagggc
     9481 ggagacagct ccgctggctc cgttcacgac agcccgcccc gccaggggga cgcccggaaa
     9541 aggcataagc aggagaacaa caaaacagac agtaatcaga gcagcagaaa aaagaagaac
     9601 agtagcagta gcagtagcag tagcagtagc aggaacaaca acatcatcag taacaggcaa
     9661 atgacaggtc tgaaaagaca gaagcccaca gcagaacgtc cgcgtaatga aaggggcggc
     9721 gcgtaagcgc ggcccctttc agcgccaccg gcgcgcggtc ttttcccggc gcgggcagac
     9781 gggcatttat cctcccgcct gcccggattc atcaggcacg cagacgaata atcatcgtcg
     9841 gaacacgggt ataagcaaat gtgccgcacg gcagcacctc gcggacatca gaaaacggta
     9901 acagcttctc ctgctggcgc ggcccgttca gacagacggc aaccagaacg ccccccggac
     9961 gcaacaggga aaaagcccgc aggatatgcc ggatatcctg cccgtgactg aacggcggat
    10021 tcataatgat ccggctgtaa tactgcaccg gctgccattc catgaagtcg ccacactgta
    10081 ccctgacacc gttaaagttt tcccgcagat accggaccag cccgctgtta atctccaccg
    10141 catcacacat ggcctccggc gcggtatccc gaatcgcccg cagtatggcc ccggtgccgg
    10201 cagagggttc cagaatgtga tcacggttgc tgatttccgc cagcgtcacc aggcgatcac
    10261 agaccggggc aggagtcaca aaaagctgag attcagcccg gatttcagca accggcgcgg
    10321 cgttatccag ccttgcgcgc atcagccgga tacgttctct taactgactg tgcatgtcat
    10381 tatctccgtc atcccccggg gatttcccgg ggggatatac cttatttctg cgggatttcc
    10441 gtgattttca tgtcggtgat atacacattc accagacgga aattattgtc cattgtgcgg
    10501 cggtagcggt atgccccgtg gtcttctgct tcttccacac tgcgcacggc cttacagtcc
    10561 cggggcaggg cggcccactg cgcctttgtc atttcccgga aaccttcccc cggatagttg
    10621 actaccggcg gacgcttcgc ggcctgactg gcgacggcag cctcttctgc cgatggtgct
    10681 ttgtagtccg tgatgcgatc gggcgtcact ttcatcgtgc cgctgtaccc gagaaaactg
    10741 taatttggcg ttgtgactga actcaccgcc ccgttgcttt tgttcacgcg gatgatagtc
    10801 agccactcgc cccggctgaa aacctgtccg cccggctcaa aatcctgtgt ccgggtaacc
    10861 acgccgccgc tttcgtccag catggcacgc tcatagatca ggcggttctg atagtggtta
    10921 acccagcgtt gctgatgctg aatctgccgt tcatgacagc ggatcgcaat ttcgcgggcc
    10981 tgctccgtgg tgatgatgtc atcatccagg gccgaccata acgaccggct cccttcatac
    11041 tggctttttt ctgccgggcg cggatacgta tccagcggga aacaggcgct gatatggtca
    11101 tgactgctga tgagttttgc catattcaga tccagcgatt cagcccgcca catcgtcaga
    11161 tatttctgcg actgtgcgat ggtcttttca gccttgcgca aatccgcttc gatttttttg
    11221 atacgacgcc agcgaacgtc cgggcgttct ttatacttcg cgtgaagcag tgccgaccgc
    11281 gcccgctcct cccagtattc cgcacgttca aagagcatca cggcacgttt catgccgttt
    11341 tcaatacgct gcgcatcacg acgggcgcgg cgttcgctgt gatgccccac aagaatgggc
    11401 tgaccgggcg ggatcatcgc ggccagtctt tccacttcat caagtgcctg tgcggattca
    11461 ctggcccgct ttccgctgta tccggtaaac cgctccgccc gtgcttcctg acgttcagcg
    11521 agcgtgctgt cttcatcctc aatctctccg gcaagtgaga ggagcacgtc ttcccggccc
    11581 ggtgtccagg ccggcgcgac aaacagcgcc tgtttgggtg cccagcgaaa accggcatca
    11641 tgcacttttt tatacgtctc cgggtcaaga cgtgacgcgg catacaggcg cagtttgttg
    11701 tcatccggtg agtaggtcgc gcggtacatc tgcacagggc tggcggcttc cggggcggtc
    11761 atggtgattg ctgctaatgt cataaaatgt tctccttccg tctgttgccg cccctcgcgg
    11821 ggcggctcac ctcatcagtt aaaagcgcgt ttcagttcga gtgtgcggag cacctgcccg
    11881 aaagccgtgt ttttcatggc ggggatgtgt acaccacgcc cccggtgaaa actccgggct
    11941 atgtgtcgcc cgaaggcttt caacacgggg tcaatttccg gcgtggggat gtcttcgcgt
    12001 ctgaaacggg gatgacggcg gggctttaca gccgctgcgg aaggtgaaac aatgcttaca
    12061 gtcataaatg gattccttat gacaaattgg ttaacaatgc cgtcaaaatt ggtcgcacaa
    12121 ttcttacggc gtggattaag caggtgcctg acctgctttt ttcatatctg catctgacgc
    12181 tttccggtgt tttcctcctt ctgttcctgc gttctcatta cctgtcagcc ggatgactga
    12241 cagaacaaat aaatgcgtat tgccggagcc tgaaataaat cctgattttt aaagaacatc
    12301 acccgttgag tttcgggagc cgcggttttg acgtttcgcg ggaacctgct gctgtattca
    12361 ctgtggtatg aacttcaccc gtttgagaac cttcgccgca aggggcatca ctgccacggc
    12421 ctgacgcacc tgtcctgggc agaacgcaaa attttttacc gggttttggg taaaaaattt
    12481 tgtgggctgt tcatttaccc tggacagtca gtgcggcagg ttgtggctgt atcagccccc
    12541 ggcgacaggg actcatacgg gtgtgtggca cccggcaggc aatgagcagg ggcccgccgc
    12601 aacgacggag agcgcgtgcg cgaaacggcg ttgcccctta catcccggcg gccttttgcc
    12661 gggatgccgg gagcgcaggc cacggcgcga cagaggcggc gcgaccggaa cggtcgctgc
    12721 aagccagggg ttttcacgca tcggaccgta gcggcagccc gaaaccgcgc cagcgggttc
    12781 ggcggagact ggcaggtgcg ccagcagctg cctgtcggag tcgggcctga cgaccggatg
    12841 ccgccaggca gccgggagcc gaaggtgcgc ccggcagaca gaacaacact gcagtagtca
    12901 gaatcagagc agtagataaa ggaacaacaa catcaggaac agttccataa cataagaaag
    12961 aaacactcct gagtcagaca catcacagac gggaagggta acgggcgacg cgccagcgtg
    13021 gcccgcttcc cttccggcag gcgtcagcct gccaggggtt ttcgcgcggg ctgacaggcg
    13081 gcctccaccg cctgcccctg tatcacctgt ttatgctgcc ctgaaggatg tagtgaccgc
    13141 cgaggaatac agcctgctga ttcgccacaa aatcgcagat ttcatcaaca atctgcgcag
    13201 tggtgagcgg tttctgcccc atttcacgac gccgcgcatt aatcacggct ttgtaatt
//
LOCUS       DAZOXR010000117        13077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZOXR010000117 DAZOXR010000000
VERSION     DAZOXR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.119"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(368..1156)
                     /gene="phoH"
                     /locus_tag="ACHUNN_002901"
     CDS             complement(368..1156)
                     /gene="phoH"
                     /locus_tag="ACHUNN_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0577217.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1777..3048)
                     /gene="efeB"
                     /locus_tag="ACHUNN_002902"
     CDS             complement(1777..3048)
                     /gene="efeB"
                     /locus_tag="ACHUNN_002902"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0577218.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3054..4181)
                     /gene="efeO"
                     /locus_tag="ACHUNN_002903"
     CDS             complement(3054..4181)
                     /gene="efeO"
                     /locus_tag="ACHUNN_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0577219.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4239..5069)
                     /gene="efeU"
                     /locus_tag="ACHUNN_002904"
     CDS             complement(4239..5069)
                     /gene="efeU"
                     /locus_tag="ACHUNN_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0577220.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5735..7243)
                     /gene="putP"
                     /locus_tag="ACHUNN_002905"
     CDS             complement(5735..7243)
                     /gene="putP"
                     /locus_tag="ACHUNN_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR0577221.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            7665..11627
                     /gene="putA"
                     /locus_tag="ACHUNN_002906"
     CDS             7665..11627
                     /gene="putA"
                     /locus_tag="ACHUNN_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0577222.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11667..12305)
                     /gene="rutR"
                     /locus_tag="ACHUNN_002907"
     CDS             complement(11667..12305)
                     /gene="rutR"
                     /locus_tag="ACHUNN_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0577223.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            12593..>13077
                     /locus_tag="ACHUNN_002908"
     CDS             12593..>13077
                     /locus_tag="ACHUNN_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0577224.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         3183 a         3608 c         3292 g         2994 t
ORIGIN      
        1 ataatattat ccctaaagaa taaatcagag gaatatattc ggtgttaaac agtcagtcaa
       61 agtatagccc attgttgatt aaacctcata agcaaaattg gcaccagatc tcctgtacag
      121 gttgaatggc cgaaaaaaga taggcagatg ctcatttttt tggcttctgg aagaaaaact
      181 ctccgcgctg gcagtattac actggtctga tgctatgacg aaaaatgcct ggtaagagta
      241 tctaaaccag actccagttc agcttatcaa cacgctcaaa gtcgcccccc tgaacgctaa
      301 cctgaagtga aaatctgttg aaataaaaca aagcccggtt cgcccgggct ctgcaccgat
      361 aacacactta actgtaggca tgcagcgtac gttggcaaag tgccgaacgt acgcagtcct
      421 ctttaccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc agcgcgtcac
      481 ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa
      541 ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca gtcacattct
      601 gcgcctcgtc aagaatgacg actgcatttt caaaggtacg tccacgcata taggcgaacg
      661 gcgcaatttc caccttccct atttccggtc gcaggcagta ctgcataaag gaagccccta
      721 agcgccggac cagcacgtcg tagaccgggc gaaaataggg agcaaacttt tctgcgatat
      781 ctccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg acgataatcc
      841 tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc caagtttttc
      901 cgcccccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc ttcagatagt
      961 gcaattgcgc ttcatttcgc gcgaggatgg gcgaagtatc gcgactgtcg cgggccatac
     1021 caatggcttc tacgccgccc atctgcacaa gcgaggtgac cgattcttct tcacgctgct
     1081 tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct ttgatcactg
     1141 ctttttgtct tcccatagag agcaccttga gttgtttgta ttcatcacac gcgccgttgg
     1201 cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca tagtttgctt
     1261 ttggataaaa tgccgaaaaa cggctacgcg caccgtttac ggcgtcggta acacatgaaa
     1321 agaaaggatg aggttgaaaa tgcaaagtga cgagatgact accggaggag aaaactccgc
     1381 gagtggtggc gcgttgatta tctaaaacat gtccagtaca ggacgttacc atccgcgatc
     1441 tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa gtacaacaga
     1501 tctcgcattt attgcaacaa tatatttact tatatttaac tataaaacac tatttcagtg
     1561 acattagttt ctactggaaa gatgacagag tgatgacagt gatgaaaaaa gctgtgtgct
     1621 ttcagcagga tttgcatcag gcatcagtgc acgatcgccg gatgcggcgt gaacgcctta
     1681 tccagcctac caaacccgca ctgattgtag gcatgataag acgcgcaagc gtcgcatcag
     1741 gcatctgcgc acaaatgcct tatccgccta aaaacattaa acccgcaata acgcgcttcc
     1801 gagataatcg ttcgcgtcct tcactcccgg cagcgaaaaa aaataaccgc cgccgatagg
     1861 tttaacgtat tcctccagcg cttcgccatt gagccttttt tgtactgtca ggaagccttt
     1921 ttccagatcg tgttggtagc agacaaacag caaccccata tccagttgtc cggagttggt
     1981 gacccccaac gaataactgt agccgcgacg cagcatcagg ctggactcac tctccgccgt
     2041 gcggggattc gccagccgga tatggctgtc cagcgcaatc accttccctt ccgggtcgct
     2101 ggcgtaatca ggcacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc
     2161 acggccaaaa atcgtctgct gttctttcag cggcgttctg tcccaaaatt ccactcgaaa
     2221 ctgaatcaag cgtactgcct gatagctgcc accgattgtc cacgcaggct cctgctgatc
     2281 tgccgttacc cacaccactt tttgcatcaa cttatcattc tggctatcgg gattggcagt
     2341 gccgtctttg aaacccagca aattaatcgg cgtctctttg cctttactac gcgacacgtg
     2401 atcggaaata aacccttccc gcttccagcg cacactgagc aaatccggcg tgtgtttgat
     2461 gatatcgcgc agcgcatgga taaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac
     2521 atcaccatga cataacgccg catccagcga gtcgttgggg aaacgcgtca tcttctgcag
     2581 cttttttggc atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga
     2641 taacgtgatg gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg
     2701 tggcaggcgc ggatttggcg tttctggtgc tgctccgccc tgagtcagaa aagcaaaacg
     2761 ctgagtcaac aagcgaaaca accgctcaag atcggcttta tcgctggcaa gcacatcaaa
     2821 cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata
     2881 aaacggctgt ttctcattgc gtgcatccgg tgaaagcgtg cccggtgcac tttgcgtttt
     2941 ttgtgcatga gcgaccggac aacttcccgc cagcgccagc gcaccaatcc ctttcagtaa
     3001 acgtcggcgt gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc
     3061 agtcccagca caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc
     3121 agtgcattcc ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg
     3181 tatttcgcca gaatggtatc gaccttttta aagttggcat cgacttttgc cagcagttcc
     3241 gggttggctt tttgcagttg tggacgcagc aaatcgacaa ttttctgcga gccttcaacg
     3301 ttagcctgga aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctaattttg
     3361 ctggctgcca cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa
     3421 gccagttcac tgatgcgttt ttgcaaatcg accacatctg tataaagctg ctcagcgtac
     3481 tgatccatcc ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca
     3541 gtgaattttg ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca
     3601 tccagatcgg agaacagttc agcaatcggt tcaatgcgct catagtgctg gcgcgtcggc
     3661 gcatacagtg ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg
     3721 gtgtcggtca ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca
     3781 ccaccaagac ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg
     3841 atcaacttcc ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt
     3901 aaattcgccg tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc
     3961 atcacgcctt tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac
     4021 tgtgttttcc cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc
     4081 actttgacct gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc
     4141 acgctcaact gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa
     4201 agtatgttgt aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc
     4261 gcccctgcgc gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag
     4321 acggcgactt cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg
     4381 ccaaacagcg agtgagttga gagcactgcg ctcatatcga aggcgatttc ctgaaagtgg
     4441 ttccacaatc cggcttcatg aaatgcgcga atggcaccag cagccagccc tgcggcgacg
     4501 aagagaataa acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc
     4561 cagtagagca ggaagcctag caccacggca gtagcaagac cgagcattgc acccagcggc
     4621 ggccagatcc cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct
     4681 tcccttgcaa cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc
     4741 tgcaatgcgc tatcgactgc ctgctccagt tgtactttga cgttgcgcga cactttgcgc
     4801 atccagaaaa ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac
     4861 agttcctgtt ctttttgcgg aaattcgccg gtggtttcgt taatgaagat acccaagccc
     4921 aggcacaacg cagcggcaag caacacgcca atccacatca cgccaatcca tcggcctcgc
     4981 tgggtacgct taagatagct ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg
     5041 cgcaacataa tgagaaacgg aacaaacatg ccacccaccc ttaaacgttt ttctgagtca
     5101 atccatgcaa agaaaagtaa atcgcagtga tagcgattat cattacggca aagaaaaata
     5161 caagcggaat gtttataaaa tgatgttgga atgtaaacaa agagaggtaa taccagggga
     5221 tataaacgat tattccaggt acaaagatgg gattgtttac aacgagccat gtgttcttgc
     5281 cagatgccgc gtaaacgcct tatccagccg acaaaactgt ccagactcaa tgcatcacag
     5341 cttccatgta aaactgataa gcgggtgtat caagcaattt cgtccatccg cgattatcaa
     5401 atctaacttt ttgcccggaa cataataaca gcgcgggtgt tggtggcgtc aaaatcgctg
     5461 aaacgttccc cgaaggccgg ggcagcccac atggatgtgg gctgagggcg cgtcttacag
     5521 gacgttacct cgcgcccgac ccggtagccg aaggggataa gctgaaggta ccgcgcagcg
     5581 gcgattttgt tcgccagagc ccgggggtgc agggggcggc ggcgactggc cgccccctgc
     5641 gcgctccctg caccattcaa tttatatggc gaggcacatt aagggagcgc aacccgctgc
     5701 tgaagccaaa caaaaccgcc gcaggctaag tcccttagtt ttcctgcaac cgtgacggcg
     5761 gagccgaatg atagtgcgca tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg
     5821 ctttacccag caaactaaac actacaatcc cgatactgcc gaagatgaag cccggaataa
     5881 tttcgtacag tcccagccag ccgaactgct tccagacgat aactgtcagc gcaccgatga
     5941 tcatccccgc cagcgcaccg ttgcgcgtca tgcgtgacca catcaccgag aacagcacca
     6001 ccggaccaaa cgccgcgcca aagcctgccc acgcgtagct cactaagccc agcacgcggt
     6061 tttccgggtt tgccgccagc gcaatcgcca ccagcgccac caccagcacc atcacacgtc
     6121 ctacccacac cagctctttc tggctggcct gtttacgcag aaacgctttg tataaatctt
     6181 cggtaatcgc actggagcac accagcagct ggcaacttaa ggttgacatt accgccgcca
     6241 gaatcgccga cagcagaatc ccggcaatcc acgggttaaa cagaatttgc gccagttcga
     6301 taaacacgcg ttcagcgttc tggtttaccg cacctgctac cgccggatgc tcgttaaagt
     6361 aagcgatacc aaagaagcca acagccaccg cccctgccag gcaaaggatc atccaggtca
     6421 tactgatacg acgcgcatgg acaatgctgt ggtgagaatc tgccgccata aaacgcgcca
     6481 ggatgtgcgg ctgcccgaag taacccagcc cccaacccat caatgagata atggcaacaa
     6541 agttcagccc tttgagcata tccacgtttt cgatgctctt ttgtttgatc acttccagcg
     6601 agtcaccaaa gccaccgaca ctgataatga cgataaccgg cgtcaggatc agggcaaaaa
     6661 tcatcaggct ggcctgtaca gtgtcagtcc agctcaccgc gaggaaaccg ccaataaagg
     6721 tgtaaaggat cgtcgacgca gctcccgcca acagagccgt ttcgtaactc atgccaaagg
     6781 tactttcaaa cagacgcgcg cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca
     6841 gcaaaataac cagcgcggag ataatgcgca aaatgcggct tttatcttca aaacgcccgg
     6901 tgaaataatc cggcaatgtt aaggcgttat tattgtattc ggtatgcaca cgcaaccgcc
     6961 cggccaccag cttccagttg atccacgcgc ctaatgtcag gccaatggcg atccagcttt
     7021 cggaaatccc ggaaagaaaa accgcgccag gcaaccccat taacagccag ccgctcatat
     7081 ccgacgcacc cgccgataat gccgtcacga atggcccaag actacgaccg cccagaatat
     7141 agtcgtcaaa gtttttcgtt gatcgccagg cgataaaccc aatcaatatc atgccaaaga
     7201 tatagacaca aaatgtcacc aacatcggtg tgctaatagc catctaaagt ctccaaaaaa
     7261 ttattatcgg caatgtcgaa acttgccgtt atatctgcca ccggaacggg gtaacagagt
     7321 ttatgtttta ccagggcgac cgtatcctgc cggaagcgct ggttattcac aatcgattta
     7381 acacaccatt tacattaaat tttagtgctc agcgacacta tttttcatca ggttgcactc
     7441 tctcacattt tttgcggttg cacctttcaa aaaggttaac tgccgcagag aaaaagtctg
     7501 agttattttt taatcactgt catatcgatt tcttttatta acatttcatt catttttaag
     7561 cttgctacgc aagtcacatt taacatggtt gcacaaagtt gcaacatcat ggatatttca
     7621 cgataacgtt aagttgcacc tttcagaaca acaggagtaa tggcatggga accaccacca
     7681 tgggggttaa gctggacgac gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg
     7741 atcgcacacc acactggtta attaagcagg cgattttttc ttatctcgaa caactggaaa
     7801 acagcgatac tctgccggag ctacctgcgc tgctttctgg cgcggccaat gagagcgatg
     7861 aagcaccgac tccggcagag gaaccacacc agccattcct cgactttgcc gagcaaatat
     7921 tgccccagtc ggtttcccgc gccgcgatca ccgcggccta tcgccgcccg gaaaccgaag
     7981 cggtttctat gctgctggaa caagcccgcc tgccgcagcc agttgctgaa caggcgcaca
     8041 aactggcgta tcaactggcc gataaactgc gtaatcaaaa aaatgccagt ggtcgtgcag
     8101 gaatggtcca ggggttattg caggagtttt cgctgtcatc gcaggaaggc gtggcgctga
     8161 tgtgtctggc agaagcgttg ttgcgtattc ccgacaaagc cacccgcgac gcgttaattc
     8221 gcgacaaaat cagcaacggt aactggcagt cacacattgg tcgtagcccg tcactgtttg
     8281 ttaatgccgc tacctggggg ctgctgttta ctggcaaact ggtttccacc cataacgaag
     8341 ccagcctctc ccgctcgctg aaccgcatta tcggtaaaag cggtgaaccg ctgatccgca
     8401 aaggtgtgga tatggcgatg cgcctgatgg gcgagcagtt cgtcacgggt gaaaccatcg
     8461 cggaagcgtt agccaatgcc cgcaagctgg aagagaaagg tttccgttac tcttacgata
     8521 tgctgggcga agctgcactg actgccgcag acgcacaggc gtatatggtt tcctatcagc
     8581 aggcgattca cgccattggt aaagcgtcta acggtcgtgg catctatgaa ggtccgggca
     8641 tttcaatcaa actgtcggcg ctgcatccgc gctacagccg cgcccagtat gaccgggtaa
     8701 tggaagagct ttacccgcgt ctgaaatcac tcaccctgct ggcgcgtcag tatgatattg
     8761 gtattaacat cgatgccgaa gaggccgatc gtctggagat ctccctcgat ctgctggaaa
     8821 atctctgttt cgagccggaa ctggcaggct ggaacggcat cggttttgtt attcaggctt
     8881 atcaaaaacg ctgcccgttg gtgatcgatt acctggttga tctcgccacc cgcagccgtc
     8941 gccgtctgat gattcgcctg gtgaaaggcg cgtactggga tagtgaaatc aagcgtgcgc
     9001 agatggacgg ccttgaaggc tatccggttt atacccgcaa ggtgtatacc gacgtttctt
     9061 atctcgcctg tgcgaaaaag ctgctggcgg tgccgaatct aatctacccg cagttcgcga
     9121 cgcacaacgc ccatacgctg gcagcgattt atcaactggc aggtcagaac tactatccgg
     9181 gtcagtacga gttccagtgc ctgcatggta tgggcgagcc actgtacgag caggtcaccg
     9241 ggaaagttgc cgacggtaaa cttaaccgac cgtgtcgtat ttatgccccg gttggcacac
     9301 atgaaacgct gctggcgtat ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt
     9361 ttgttaaccg tattgccgac acctctttgc cactggatga actggtcgcc gatccggtca
     9421 ctgctgtaga aaaactggcg caacaggaag ggcaaactgg attaccgcat ccgaaaattc
     9481 ccctgccgcg cgatctttac ggtcacgggc gcgacaactc ggcagggctg gatctcgcta
     9541 acgaacaccg tctggcctcg ctctcctctg ccctgctcaa tagtgcactg caaaaatggc
     9601 aggccttgcc aatgctggaa caaccggtag cggcaggtga gatgtcgccc gttattaacc
     9661 ctgcggaacc gaaagatatt gtgggctttg tgcgtgaagc cacgccacgt gaagtagaac
     9721 aggcgctgga aagtgcggtt aataacgcgc caatctggtt tgccacgcct ccggctgaac
     9781 gcgcggcgat tttgcatcgc gctgccgtgc taatggaaag ccagatgcag caactgattg
     9841 gtattctggt gcgtgaagcc ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag
     9901 cggtcgattt tctccactac tacgccggac aggtgcggga tgatttcgct aacgaaaccc
     9961 accgtccatt agggcctgtg gtgtgtatca gtccgtggaa cttcccgctg gctattttca
    10021 ccgggcagat cgctgccgca ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac
    10081 aaacgccgct gattgccgcg caagggatcg ccattttgct ggaagcgggt gtaccccctg
    10141 gcgtggtgca attgctgcca ggtcaggggg aaaccgtggg cgcgcaactg acgggtgatg
    10201 atcgcgtgcg cggggtgatg tttaccggtt caaccgaagt cgctacgtta ctgcagcgca
    10261 atatcgccag ccgcctggac gctcagggtc gccctattcc gctcatcgct gaaaccggcg
    10321 gcatgaacgc gatgattgtc gattcttcag cactgaccga acaagtcgtc gtggatgtgc
    10381 tggcctcggc gttcgacagt gcgggtcagc gttgttcggc actgcgcgtg ctgtgcctgc
    10441 aagatgagat tgccgaccac acgttgaaaa tgctgcgcgg tgcaatggcc gaatgccgga
    10501 tgggtaatcc gggtcgcctg accaccgata tcggtccggt gattgatagc gaagcgaaag
    10561 ccaatattga gcgccatatt cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg
    10621 tgcgggaaaa cagcgaagat gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc
    10681 tgatcgaact ggatgacttt gccgaattgc acaaagaggt ctttggtccg gtgctgcatg
    10741 tggtgcgtta caaccgtaac cagctaccag agctgatcga gcagattaac gcttccggct
    10801 atggtctgac gcttggtgtc catacgcgta ttgatgaaac catcgcccag gtcactggct
    10861 cggcccatgt tggtaacctg tatgttaacc gtaatatggt gggcgcagtg gttggtgtgc
    10921 agccgttcgg cggcgaaggg ttgtccggta ccgggccgaa agcaggcggt ccgctctatc
    10981 tctaccgtct gctggcgaat cgcccggaaa gtgcgctggc agtgacgctc gcgcgtcagg
    11041 atgcagagta tccggtcgat gcacagttga aagccgcatt gactcagccg ctaaatgcac
    11101 tgcgggaatg ggcagcaaat cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc
    11161 tggcgcaggc aggaacacaa cgattgctgc cggggccgac gggtgaacgc aacacctgga
    11221 cgctgctgcc gcgtgagcgc gtgttgtgta ttgccgatga tgagcaggat gcgctgactc
    11281 agctcgccgc cgtgctggcg gtgggcagcc aggtactgtg gccggatgac gcgctgcatc
    11341 gtcagctagt aaaggcattg ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg
    11401 aaaatataac cgctcaaccc tttgatgcgg tgatcttcca cggtgattcg gatcagcttc
    11461 gcgcattgtg tgaagccgtt gctgcgcggg atggcgcaat tgtttcggtg cagggttttg
    11521 cccgtggcga aagcaatatc cttctggaac ggctgtatat cgaacgttcg ctgagtgtga
    11581 ataccgctgc cgctggcggt aacgccagtt taatgacgat cggttaaaca ggtagccgga
    11641 ggatgttaca acctcctccg gcatctttaa cgtggtcgaa tcccctcaat aataatccgc
    11701 tgcacgtttt caaccgtttg attgaaaaat atctcatcgc gcaacgtcgc gcctgtcacc
    11761 gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg aagcccaaat catgaaaatc
    11821 aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga cccaaccggc aatcagcgca
    11881 gatttctcat caattaatgc cttcaaatcg cccgtcagtt catccattaa cagcggcgcg
    11941 cctgccagca tctccataca gaacaggcgc gaagcctgcg gataatcgcg ggagacttcc
    12001 agcttcagac ggatgtactc ttttatcgcc gccagcggag cgaaatcttc acgaaacgct
    12061 tttaacggtg ccagccagat atcgagaatc tgccgcagca cggcaatata cagcgcctct
    12121 tttgacggaa agtaatacag cagattggtt tttgaaacac ccgccaactc tgcgatctgc
    12181 tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc
    12241 gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc
    12301 gtcatgcgct ctcccctctt gtagattggc aacaggatag caaaaccccc gcccggcctc
    12361 gaccttctgc actctcatcg cgcacagatg catgttttat gtgcaactgt tttgaccgtt
    12421 tagtccactt tttatcagat attttagata acgtaatatt aacatattaa taacattaac
    12481 tttttaaaaa ctggcatccg ctttgcaaac aagccaatac gcggctgaaa aggatatgca
    12541 ggatgcagcg ccccgtctga ctttcacttt acgagacgaa gagaggttga tgatgaaaat
    12601 tggcgtattc gtacctattg gcaacaacgg ctggctcatt tcgacccacg cgccgcagta
    12661 catgccgacc tttgaactga ataaagccat tgtgcagaaa gcggagcatt accatttcga
    12721 tttcgccctg tcgatgatca aactgcgtgg ctttggcggc aaaactgagt tctgggatca
    12781 caaccttgag tcgttcacct tgatggcggg tctggcagcc gtgacctcgc gcattcagat
    12841 ttacgccact gctgccacct taacgttacc tccggcaatc gtcgcccgta tggccgcaac
    12901 catcgactcc atctctggcg ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc
    12961 cgtgtatgag cagatgggta tctggcctgg cgatgactat ttctcccgtc gttacgacta
    13021 tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc acggggaaaa gcgattt
//
LOCUS       DAZOXR010000118        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZOXR010000118 DAZOXR010000000
VERSION     DAZOXR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.120"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHUNN_002909"
     CDS             <1..191
                     /locus_tag="ACHUNN_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577225.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHUNN_002910"
     CDS             191..1393
                     /locus_tag="ACHUNN_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR0577226.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUNN_002911"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUNN_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0577227.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHUNN_002912"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHUNN_002912"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0577228.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUNN_002913"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUNN_002913"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0577229.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUNN_002914"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUNN_002914"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0577230.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUNN_002915"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUNN_002915"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0577231.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUNN_002916"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUNN_002916"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0577232.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUNN_002917"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUNN_002917"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0577233.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZOXR010000119        12881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZOXR010000119 DAZOXR010000000
VERSION     DAZOXR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.121"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUNN_002918"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUNN_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0577234.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHUNN_002919"
     CDS             1598..4654
                     /locus_tag="ACHUNN_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0577235.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHUNN_002920"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHUNN_002920"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0577236.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHUNN_002921"
     CDS             5161..5349
                     /locus_tag="ACHUNN_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0577237.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUNN_002922"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUNN_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0577238.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUNN_002923"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUNN_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0577239.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASELGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUNN_002924"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUNN_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0577240.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUNN_002925"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUNN_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0577241.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUNN_002926"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUNN_002926"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0577242.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUNN_002927"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUNN_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0577243.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUNN_002928"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUNN_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0577244.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUNN_002929"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUNN_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0577245.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3219 a         3194 c         3472 g         2996 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 aatcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acttggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt g
//
LOCUS       DAZOXR010000120        12801 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZOXR010000120 DAZOXR010000000
VERSION     DAZOXR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.122"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(175..1062)
                     /gene="rarD"
                     /locus_tag="ACHUNN_002930"
     CDS             complement(175..1062)
                     /gene="rarD"
                     /locus_tag="ACHUNN_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0577246.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1114..1581)
                     /gene="yigI"
                     /locus_tag="ACHUNN_002931"
     CDS             complement(1114..1581)
                     /gene="yigI"
                     /locus_tag="ACHUNN_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0577247.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1746..2615
                     /gene="pldA"
                     /locus_tag="ACHUNN_002932"
     CDS             1746..2615
                     /gene="pldA"
                     /locus_tag="ACHUNN_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0577248.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2748..4577
                     /gene="recQ"
                     /locus_tag="ACHUNN_002933"
     CDS             2748..4577
                     /gene="recQ"
                     /locus_tag="ACHUNN_002933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0577249.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4641..5258
                     /gene="rhtC"
                     /locus_tag="ACHUNN_002934"
     CDS             4641..5258
                     /gene="rhtC"
                     /locus_tag="ACHUNN_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0577250.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5320..5940)
                     /gene="rhtB"
                     /locus_tag="ACHUNN_002935"
     CDS             complement(5320..5940)
                     /gene="rhtB"
                     /locus_tag="ACHUNN_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0577251.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6051..7073
                     /gene="pldB"
                     /locus_tag="ACHUNN_002936"
     CDS             6051..7073
                     /gene="pldB"
                     /locus_tag="ACHUNN_002936"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0577252.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7081..7881
                     /gene="yigL"
                     /locus_tag="ACHUNN_002937"
     CDS             7081..7881
                     /gene="yigL"
                     /locus_tag="ACHUNN_002937"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0577253.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7957..8856
                     /gene="bioP"
                     /locus_tag="ACHUNN_002938"
     CDS             7957..8856
                     /gene="bioP"
                     /locus_tag="ACHUNN_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0577254.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8744..9697)
                     /gene="metR"
                     /locus_tag="ACHUNN_002939"
     CDS             complement(8744..9697)
                     /gene="metR"
                     /locus_tag="ACHUNN_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0577255.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9933..12194
                     /gene="metE"
                     /locus_tag="ACHUNN_002940"
     CDS             9933..12194
                     /gene="metE"
                     /locus_tag="ACHUNN_002940"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0577256.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12316..12438)
                     /locus_tag="ACHUNN_002941"
     CDS             complement(12316..12438)
                     /locus_tag="ACHUNN_002941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577257.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12479..12724)
                     /locus_tag="ACHUNN_002942"
     CDS             complement(12479..12724)
                     /locus_tag="ACHUNN_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR0577258.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3010 a         3225 c         3430 g         3136 t
ORIGIN      
        1 ggttttgtca aatccttggt agatatacga tgtctctagg atggagtaag tactggggag
       61 ttttgggggt atttcctgta acaaatattg ttctttttct tatcttaatt tttttcaaaa
      121 aaagataaat aagtcagtgg gtaggctctt taaacggcct acccatgtta attcttaact
      181 ttttctacgc tgcgtataaa tcgcatccat cacaaaaatt gccagcgcca cccaaataaa
      241 ggcgaaagtc accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag
      301 gaacatcagc gtcgggccaa tgtactggaa aaagcctaac gttgagagac gcaagcgcgt
      361 ggcagcggcg gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag
      421 taaattcagc gacatcgggt tttgccccat atggctggtt gagctgtcgg caatagcaaa
      481 caggtaaatt gccgccacgg gcagcagcca catggtttcg attaacatgc cggtttgcgc
      541 ttcaacggca atcttcttgc gtaccagacc gtagaaggca aaactaaatg ccagtcccag
      601 cgcgataata ggtagcgaac caaaagtcca cagctggact aacacgccac atatcgccag
      661 aatcaccgcc agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac
      721 aatgttcacc agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt
      781 caccgcccag ataaacagta gccagttgcc accaatcagc acggcagaga ctgccagcat
      841 aaaaattttc tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct
      901 catcagcacc accataaaga aaaacgacca gatcacgcga tgcgtcagga tttcatcggc
      961 gggcacgtag taaatcaact tgaagtacgc tggcgctata ccccaaataa aataagcggc
     1021 aagagcgagt aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta
     1081 attggtaaca gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc
     1141 actggcaata taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt
     1201 gcctgcacgc aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata
     1261 atcaacgcga agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact
     1321 gatggtttcg tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag
     1381 cgccgacgca atgaccccgc cgtgcaaaat gctttgcgcc cagttgccca ccatcattgg
     1441 ctgattttta aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa
     1501 tgcgcggtta aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc
     1561 agcggtcagt acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt
     1621 tatgcttctt tgttgttggt ttctacttta ggaagggata actaacggct atggagttaa
     1681 gtatgtaaaa tagcccgcag aaaaatattc accttatcaa taattcgtta cggagaacac
     1741 gaccgatgcg gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat
     1801 atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta
     1861 tcgccaatat gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact
     1921 acctcattta cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg
     1981 cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc
     2041 gtgggatttt aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc
     2101 aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt
     2161 tcctcggttt tgccaccgat tactgttttg caggttggac gctgcgcgat gtggagatgg
     2221 ggtataacca cgactctaac gggcgttccg acccgacctc ccgcagctgg aaccgccttt
     2281 atactcgcct gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg
     2341 tgggtaatac tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa
     2401 tcggctatca cctcggtgat gcggtgttga gtgcgaaagg acagtacaac tggaataccg
     2461 gttacggcgg cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata
     2521 ctcaggttta cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg
     2581 tcggtgtagg agttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc
     2641 tggaacccag catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca
     2701 ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg ggggaatgtg gcgcaggcgg
     2761 aagtgttgaa tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac
     2821 agtttcgccc cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg
     2881 tcgtcatgcc caccggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa
     2941 acggccttac cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac
     3001 tgcaagccaa cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gagcagcaac
     3061 ttgaagtgat gacaggctgc cgcaccgggc aaattcgtct gctttatatc gccccggaac
     3121 gcctgatgct ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg
     3181 ttgatgaagc gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc
     3241 ttggtcagtt acgccagcgt ttcccgaccc tgccgtttat ggcgctgact gccacagccg
     3301 acgacaccac gcgccaggat atcgtgcgct tgctggggct gaacgatccg ctgattcaaa
     3361 tcagcagttt tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg
     3421 atcagttgat gcgctacgtg caggaacagc gcggtaagtc cggcattatc tactgcaaca
     3481 gccgggcgaa agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg
     3541 cctatcatgc cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg
     3601 atgacctgca aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg
     3661 ttcgcttcgt ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg
     3721 gtcgcgccgg gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata
     3781 tggcgtggct gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatattgagc
     3841 gccacaaact caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat
     3901 tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg
     3961 atccgccgaa acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc
     4021 gtgtgaatca gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc
     4081 agcgtatccg cgactatggt catgacaaac tgaaagtcta tggcatgggc cgtgataaaa
     4141 gccatgaaca ttgggtgagc gtgatccgcc agctgattca cctcggcctg gtgacgcaaa
     4201 atattgccca gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg
     4261 aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga
     4321 aatcgttcgg cggcaactat gatcgtaaac tgttcgccaa attacgcaaa ctgcgtaaat
     4381 cgattgccga tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg
     4441 agatggctga acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga
     4501 tgcgcaagct ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg
     4561 gcgacgacga agagtagtca gcagcataaa aaagtgccag gatgaagact ccgtaaacgt
     4621 ttcccccgcg agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg
     4681 tggcgcttat gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt
     4741 cccgtaaaga agcgatgatg ggcgtgctgg gcattacctg cggtgtaatg gtttgggctg
     4801 ggattgcgtt gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctta
     4861 tggttggcgg tggcctgtat ctctgctgga tgggttacca gatgctgcgc ggcgcgctga
     4921 aaaaagaggc ggtttctgca cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt
     4981 tcctgaaagg tttactgacc aatctcgcta atccgaaagc gattatctac tttggctcgg
     5041 tgttctcatt gtttgtcggt gataacgttg gcactaccgc gcgctggggc atttttgcgc
     5101 tgatcattgt cgaaacgctg gcgtggttta tcgtcgttgc cagcctgttt gccctgccgc
     5161 aaatgcgccg tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat
     5221 ttgccggatt tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta
     5281 agtcggataa ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc
     5341 taacagcgct cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg
     5401 ctttggtcct ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac
     5461 aataatatcg accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg
     5521 ttgcggcatg atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt
     5581 gagattcaca aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga
     5641 tttaaggtca attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc
     5701 cgcgcctgcc cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa
     5761 ccccacgcca accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc
     5821 caccgcgccg cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc
     5881 tggcgacagg cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat
     5941 gatgaactcc cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc
     6001 aggcacgcta aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc
     6061 agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga
     6121 ctgatttctg gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc
     6181 gctttgtccg ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta
     6241 ttgagagcta cgtaaaatat gcggaactgg cctatgacct gttccatttg gggtttgatg
     6301 tcttaatcat cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc
     6361 tcgggcatgt taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg
     6421 aggttcagcc cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga
     6481 tctccacatt atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc
     6541 caatgtttgg gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg
     6601 ccgaagcgca tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt
     6661 tgccgtttgc tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct
     6721 tctatgctga tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa
     6781 gtattctggc tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc
     6841 tcttgcaggc tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac
     6901 tccgcaccgc cgcgggccat cctgtcgaag gaggacgacc gttggtaatt aaaggtgctt
     6961 accatgagat cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccattgttg
     7021 attttttcaa caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt
     7081 atgtaccagg ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta
     7141 tccccttacg ccaaagaaac cctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt
     7201 gcgaccggtc gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gatcaagtcg
     7261 tacatgatca cctccaacgg cgcgcgcgtt cacgatctgg atggcaatct gatttttgct
     7321 cataacctgg atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatctggac
     7381 atcattacta acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag
     7441 atgcgctttt ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag
     7501 ccggaaggcg tcagcaaagt gttcttcacc tgcgattccc atgaacaact gctgccgctg
     7561 gagcaggcga ttaacgctcg ttggggcgat cgcgtcaacg ttagtttctc taccttaacc
     7621 tgtctggaag tgatggcggg cggcgtttca aaaggccatg cgctggaagc ggtggcgaag
     7681 aaactgggct acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgatgccgaa
     7741 atgctgtcga tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac
     7801 cttcatcccg agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg
     7861 cgtaaactct atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc
     7921 tacaatggat actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg
     7981 attctgtggg ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc
     8041 tattttgcgg tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt
     8101 acccgtggca atagcctgaa aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc
     8161 ggcgtgatgt atatgctgag tttccgcgct tatctctacc tgacggtctc cgaattgttg
     8221 ctgttcaccg tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc
     8281 cgtctgcgct ggggctatgc ttttagcgcc ttgctggcgg tgattggtgc cgggattatt
     8341 cgctatgatc aggttaccga ccatttctgg actggcttgt tgctggtgca actctccaat
     8401 atcacttttg ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg
     8461 cagcataacg cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg
     8521 ttcttgctgg gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg
     8581 tttcttggcg tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag
     8641 gtggacgccg gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggta
     8701 aacctggcta tctggcacca acagccgcac tggccaacgt tcattacagg cgcgctggtg
     8761 atcctggcct cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca
     8821 gatgatcgca ggcgtgattg cgcgctgagc gaataaatgc ttctgtaatc ggctgacgct
     8881 gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg
     8941 tcaccaccag accttggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac
     9001 ccatccgcgc ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg
     9061 ggctgacgcc tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg
     9121 gataaatcaa cagcgtctcg ctggcgagat cttccggtgt aattcgcgtt ttcgccgcca
     9181 gtggatggtc aggtgctaac accagacgca cttcatagtc gaacatcggc gaataatgca
     9241 ggccactgcg cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg
     9301 caggctgcgg gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct
     9361 tatggaaatt ttctaacgcg ggcgtcagcc actgaataca gctatggcac tcaatggcaa
     9421 tgcgcagacg cgtctgctgc ggttcattgc aggcttgcag tgcctggctg atttgcggca
     9481 gcacctggtt tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc
     9541 tcttacgcac aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca
     9601 gggcggattg cgtctgatgc aacgtcgccg cagcggctgc gagagagccg cagttccgca
     9661 acgcttgtag cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga
     9721 ataatttgcg cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac
     9781 ggctaaaatc cttcgtcttt taaatttatg gtgcgttggc tgcgtttctc caccccggtc
     9841 acttacttca gtaagctccc ggggatgaat aaacttgccg ccttccctaa attcaaaatc
     9901 cataggattt acatataatt agaggaagaa aaatgacaat attgaatcac accctcggtt
     9961 tccctcgcgt tggcctgcgt cgcgagctga aaaaagcgca agaaagttat tgggcgggga
    10021 actccacgcg tgaagaactg ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc
    10081 aacaaaagca agcgggtatc gacctgctgc cggtgggcga ttttgcctgg tacgatcatg
    10141 tactgaccac cagtttgctg ctgggtaatg ttccgccacg tcatcagaac aaagacggtt
    10201 cggtagatat cgacaccctg ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg
    10261 cggcggcagc ggaaatgacc aaatggttta acaccaacta tcactacatg gtgccggagt
    10321 tcgttaaagg ccaacagttc aaactgacct ggacgcagct gctggaggaa gtggacgagg
    10381 cgctggcgct gggccacaac gttaagcctg tgctgctggg gccggttact tacctgtggc
    10441 tggggaaagt gaaaggtgaa cagtttgacc gccttagcct gctgaacgac attctgccgg
    10501 tttatcagca agtgctggca gaactggcga aacgcggcat cgagtgggta cagattgatg
    10561 aacccgcgct ggtactggaa ctaccacagg cgtggctgga cgcatacaaa cccgcttacg
    10621 acgcgctcca gggacaggtg aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa
    10681 atctcgacac gattactgcg ctgcctgttc agggtctgca tgttgatctc gtacatggta
    10741 aagatgacgt tgctgaactg cacaagcgcc tgccttctga ctggttgctg tctgcgggtc
    10801 tgatcaatgg tcgtaacgtc tggcgcgccg atcttaccga gaaatatgcg caaattaagg
    10861 acattgtcgg caaacgtgat ttgtgggtgg catcttcctg ctcgttgctg cacagcccca
    10921 tcgacctgag cgtggaaacg cgtcttgatg cagaagtgaa aagctggttt gccttcgccc
    10981 tacaaaaatg ccatgaacta gcactgctgc gcgatgcgct gaacagtggt gacacggcag
    11041 ctctggcaga gtggagcgcc ccaattcagg cgcgtcgtca ctctactcgt gtacataatc
    11101 cggcggtaga aaagcgtctg gcggcgatca ccgcccagga cagccagcgt gcgaatgtct
    11161 atgaagtgcg tgctgaagcc cagcgtgcac gttttaaact gccagcgtgg ccgaccacca
    11221 cgattggttc cttcccgcaa accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg
    11281 gcaatctcga cgccaataac taccgcacgg gcattgcgga acatatcagg caggccattg
    11341 ttgaacagga acgtttggga ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca
    11401 tggtggaata ctttggcgag cacctcgacg ggtttgtctt tacgcaaaac ggttgggtac
    11461 agagctacgg ttcccgctgc gtgaagccac cgattgttat tggtgacgtt agtcgcccgg
    11521 caccgattac cgtggagtgg gcgaagtatg cgcaatctct gactgataaa ccggtgaaag
    11581 ggatgctgac tggcccggtg actattctct gctggtcgtt cccgcgtgaa gatgtcagcc
    11641 gtgaaaccat cgccaaacag attgcgctgg cgctgcgtga tgaagtggcc gatctggaag
    11701 ccgctggaat tggcatcatc cagattgacg aaccggctct gcgcgaaggt ttaccgctgc
    11761 gtcgtagcga ctgggatgcg tatctccagt ggggcgtaga ggccttccgt atcaacgccg
    11821 ccgtggcgaa agatgacaca caaatccaca ctcacatgtg ttattgcgag ttcaacgaca
    11881 tcatggattc gattgcggca ctggacgcag acgtcatcac catcgaaacc tcgcgttctg
    11941 acatggagtt gctggagtcg ttcgaagagt ttgattatcc gaatgaaatc ggtccaggcg
    12001 tctatgacat tcactcgcca aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga
    12061 aagcggcaaa acgcattccg gcagagcgcc tgtgggttaa cccggactgt ggcctgaaaa
    12121 cgcgcggctg gccagaaacc cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt
    12181 tgcgtcgggg atgatgaata ttaacgagtc actgtaaatt gcagtacgat gaaagaggca
    12241 aaactgcctg atactaggcc tacataattt atgcaattta ttgaattttt gggttttgtt
    12301 ggccggacaa ggcgttcacg ccgcatccgg catgaacaac gcgcactttg ccaataatct
    12361 gatggcttcc atcaccggaa gccattttgc tatctgcgcc cgttcagata cagaaataac
    12421 cgaatcattt ttacgcaaaa tataatcctg agcgatttgt tcagtaatca acgctacgtt
    12481 atttattctc acttttcacg aaattgtatc ccttttcagt aacgtggtag tgaggcatag
    12541 acttatgtcc gctatcctca ataataacca aaccctcgtt agctaatttg ttaacaatgt
    12601 taacaacatt accgaatcct ggaatttgcc ttatggatag tgtgcgatcg agattcatcc
    12661 agttccagtt tatatctttc tcagccataa ttttcagtac gttaatttca gtttcagtta
    12721 tcatagtcaa gtgcatcctg gtggtataag tcattaagtt ctcccttaag tttataatct
    12781 tctagagtag cagtatgcgt a
//
LOCUS       DAZOXR010000121        12762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZOXR010000121 DAZOXR010000000
VERSION     DAZOXR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.123"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            140..643
                     /locus_tag="ACHUNN_002943"
     CDS             140..643
                     /locus_tag="ACHUNN_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0577259.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHUNN_002944"
     CDS             complement(722..973)
                     /gene="yecJ"
                     /locus_tag="ACHUNN_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0577260.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHUNN_002945"
     CDS             complement(1088..1174)
                     /gene="azuC"
                     /locus_tag="ACHUNN_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0577261.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1437..1760
                     /locus_tag="ACHUNN_002946"
     CDS             1437..1760
                     /locus_tag="ACHUNN_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0577262.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1931..2428
                     /gene="ftnA"
                     /locus_tag="ACHUNN_002947"
     CDS             1931..2428
                     /gene="ftnA"
                     /locus_tag="ACHUNN_002947"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0577263.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2466..2705)
                     /locus_tag="ACHUNN_002948"
     CDS             complement(2466..2705)
                     /locus_tag="ACHUNN_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0577264.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            2896..4107
                     /gene="tyrP"
                     /locus_tag="ACHUNN_002949"
     CDS             2896..4107
                     /gene="tyrP"
                     /locus_tag="ACHUNN_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0577265.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4169..4834)
                     /gene="yecA"
                     /locus_tag="ACHUNN_002950"
     CDS             complement(4169..4834)
                     /gene="yecA"
                     /locus_tag="ACHUNN_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0577266.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5030..5116)
                     /locus_tag="ACHUNN_002951"
     tRNA            complement(5030..5116)
                     /locus_tag="ACHUNN_002951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5080..5082),aa:Leu,seq:taa)
     gene            complement(5129..5202)
                     /locus_tag="ACHUNN_002952"
     tRNA            complement(5129..5202)
                     /locus_tag="ACHUNN_002952"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5168..5170),aa:Cys,seq:gca)
     gene            complement(5257..5332)
                     /locus_tag="ACHUNN_002953"
     tRNA            complement(5257..5332)
                     /locus_tag="ACHUNN_002953"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5297..5299),aa:Gly,seq:gcc)
     gene            complement(5484..6032)
                     /gene="pgsA"
                     /locus_tag="ACHUNN_002954"
     CDS             complement(5484..6032)
                     /gene="pgsA"
                     /locus_tag="ACHUNN_002954"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0577267.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6089..7921)
                     /gene="uvrC"
                     /locus_tag="ACHUNN_002955"
     CDS             complement(6089..7921)
                     /gene="uvrC"
                     /locus_tag="ACHUNN_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0577268.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7918..8574)
                     /gene="uvrY"
                     /locus_tag="ACHUNN_002956"
     CDS             complement(7918..8574)
                     /gene="uvrY"
                     /locus_tag="ACHUNN_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0577269.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8870..9046
                     /gene="yecU"
                     /locus_tag="ACHUNN_002957"
     CDS             8870..9046
                     /gene="yecU"
                     /locus_tag="ACHUNN_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0577270.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9033..9257
                     /gene="yecF"
                     /locus_tag="ACHUNN_002958"
     CDS             9033..9257
                     /gene="yecF"
                     /locus_tag="ACHUNN_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0577271.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9324..10046)
                     /gene="sdiA"
                     /locus_tag="ACHUNN_002959"
     CDS             complement(9324..10046)
                     /gene="sdiA"
                     /locus_tag="ACHUNN_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR0577272.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10276..11028)
                     /gene="tcyN"
                     /locus_tag="ACHUNN_002960"
     CDS             complement(10276..11028)
                     /gene="tcyN"
                     /locus_tag="ACHUNN_002960"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0577273.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(11025..11693)
                     /gene="tcyL"
                     /locus_tag="ACHUNN_002961"
     CDS             complement(11025..11693)
                     /gene="tcyL"
                     /locus_tag="ACHUNN_002961"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0577274.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11708..12694)
                     /gene="dcyD"
                     /locus_tag="ACHUNN_002962"
     CDS             complement(11708..12694)
                     /gene="dcyD"
                     /locus_tag="ACHUNN_002962"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0577275.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         3186 a         3269 c         2984 g         3323 t
ORIGIN      
        1 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
       61 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      121 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      181 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      241 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      301 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      361 tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      421 cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga
      481 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gtctgttgct
      541 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
      601 cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat
      661 gcattgcgcc gatgaaggtt ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt
      721 tttaatggga ttcaccctgt ggggtaaact tgagttcaat aagcgcgatg gctttttgga
      781 ttgcccgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttca
      841 gctgactgga cattttttca cgaacttcaa cgggcgcgat tacatcgaga acatccagaa
      901 tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca
      961 gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca
     1021 catcttcagc gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccggagcagg
     1081 ttttttgtta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat
     1141 tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac
     1201 aggtgttatt gtctttgcgc ttataatatg acaaccatca caaaaatcaa tctttatgtg
     1261 atacaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaaa aacagccccc
     1321 ctatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt ttccggccat
     1381 tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc tggaccatga
     1441 gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccaag
     1501 cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat gaccaggctt
     1561 ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc
     1621 agcaggaagg ctatacccac gcggtcccct ttggtcagcc agtaggcaac tgtagccttt
     1681 ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg
     1741 ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg attaaagaaa
     1801 tagcggtaat gcgttttatt cccattcgta tttttaataa ttgaagttta tattttacct
     1861 tttgcaaata ataaaataac aaattatagt gacgccacag caacaaatat aacctttgtg
     1921 gagcactatc atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga
     1981 actgtactct tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga
     2041 aggtgctgcc gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct
     2101 gtttgattac ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt
     2161 tgctgaatat tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat
     2221 cacccagaaa attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt
     2281 taatttcctg cagtggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat
     2341 tattgataaa ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga
     2401 actctctacc ctcgacacac aaaactaatg ctctccggcg gcaggtttac tgccgccgtt
     2461 tcaaatcagt gacggcaaat cttactctga tcggtcgaaa aaccgtcttt cgaaggtata
     2521 aatttgcctt ttgctgccag aaacgctacc agctctcccg ctgtcatccc ttctgccgag
     2581 caggtgtgaa aacgtgcctg ttcaccaaaa cgcgctttaa tcgcagcttc cagactggca
     2641 tgcgtatatt gctcgcctga ttcaatcatc atatttaaca cttcatgacc gtgaatagag
     2701 tccatcgttc ctcctcaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg
     2761 ccagcgcagg actgaatgct ttttattgta catttatatt tacactatat gtaacgtcgg
     2821 tttgacgaag cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc
     2881 aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg tttttatcgt ggcgggaacc
     2941 acaattggcg caggcatgct ggcaatgccg ctggctgcgg ccggtgttgg ttttagcgtt
     3001 acgttaatct tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg
     3061 gaggtgtacc agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg
     3121 ggacgctacg gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact
     3181 gcggcataca tcagcggtgt cggtgaattg ttggcctcca gcatcagcga ctggacaggt
     3241 atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt
     3301 tgtgttggaa catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt
     3361 tttctggtgg tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc
     3421 ctgccgttgc aacaggggct ggctctgtca gcaatcccgg tgatttttac ctcatttggt
     3481 tttcacggta gcgtgccgag tattgtcagc tatatggatg gcaacgttcg taagctacgc
     3541 tgggtgttta taaccggtag tgcgatcccc ctggtggcat atattttctg gcaggtggca
     3601 acgcttggca gcattgattc aacaaccttt atgggattgc tggctaatca tgctggatta
     3661 aacgggctgt tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg
     3721 catttatttg ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt aggcttattt
     3781 gattatctgg ctgatttatt tcagcgttca aataccgttg gtggacggtt gcaaactggt
     3841 gcaattacct ttctgccgcc gttggcgttt gcactgtttt atccacgagg atttgtgatg
     3901 gcgctgggtt acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg
     3961 acctggcaaa gcagaaagca caatcctcag gcgggttacc gggtcaaagg tggtcgtccg
     4021 gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg
     4081 gcagggttgt taccagaagt ggggtgatca gatagcctca aattccttat tgggtgccag
     4141 aattaacgct gacacccaat ttggcctctt aatgcaggca gcactgctta aatttcttac
     4201 cactaccgca cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct
     4261 gaacagcttt ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc
     4321 gaatggcatc tacgctctct tcaaacgctt ctggcgacat cttttctacc cgctcgaagt
     4381 tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg
     4441 ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg
     4501 tcagctcgct gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt
     4561 cgttcagacg ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca
     4621 tctctttctc tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc
     4681 actgttccgg ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct
     4741 ccgccacatc aaggatggcg tggttagtgt tgtatttggt cagaatatcg tccagccatt
     4801 ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc
     4861 gccagcacct atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg
     4921 tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg
     4981 tggtttcacg acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga
     5041 gcgggacttg aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg
     5101 attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag
     5161 acggatttgc aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc
     5221 agccagacat ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg agactcgaac
     5281 tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc gcattcatca
     5341 agcaatcagt taatcacttg attttattat cgtctggcaa tcagtgccgc cgttcgatgc
     5401 gttgcattct acttacctgg cgcgatgagt caacgatatt tttcaccact tttgatcgtt
     5461 tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa
     5521 tattgcaaca ttgaccacag agtcagtacc gcagccacaa agaaaagtgc aataccggcg
     5581 tactcaaccc aaatgttcgg acgccacagc agccatgcca acgccaccat ctgggcagtg
     5641 gttttcactt tcccaatcca ggagacagcc acgctactgc gtttacccaa ctccgccatc
     5701 cattcgcgta gcgcagaaat aataatttca cgggcgatca tcgttgctgc cggtaaggtc
     5761 acccaccagc tgtggtaatg ctcggttacc agcaccatgg cgatagccac gagaacttta
     5821 tctgctacag ggtcaaggaa agcaccaaac cgggtactct ggttccagcg gcgtgccaga
     5881 aaaccatcga accagtcagt caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc
     5941 gaccaggtga caggcagata aaagaccaat acaaagaatg ggataaggat gacacggaac
     6001 agtgtaagca acgtagggat attaaattgc ataatgacgg gtaactatct gttgtcagta
     6061 agattacccc tatgttgcta cagagacatc aatgtttcaa cgaccagaag atcttttctg
     6121 ccagaccttg cgaaataccc ggcacttttg caatttcctc gacgctggcg ttacgtaaac
     6181 cttgcaaacc gcccatatat ttcaacaaca tttgccgacg ttttggcccg acgccttcaa
     6241 tggtttccag ggaactggta tttttgacct tcgcccgttt tttacggtgc ccgccaatcg
     6301 cgtgatcatg tgattcatcg cgaatatgct ggataacatg cagcgcggga gaatctggcg
     6361 gcaaactgaa tccctcacct tccggctcaa agaacagcgt ttccagccca gccttacgat
     6421 ctgctccttt ggcaacgcca agtagcagcg gatgattttt atcccatgag acatccagtt
     6481 cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata aggatcacat
     6541 ccgggatctt actgtcgtca atggctttac cataacgccg acgcagcacc tgattcatcg
     6601 ccgcataatc atcgcccggc gtgatgcctg taatgttata gcgccgatac tccgcacgca
     6661 gcgggccgtt agcatcaaac accacacagg aagcgacggt ttgttcgccc atggtatggc
     6721 tgatgtcaaa gcactccatc cgcttcactt ccggcaattt caacacgctg gcaagcgcgg
     6781 tcagtcgctg gtgaacggta gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat
     6841 tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg aggtttggtt tgaacattaa
     6901 tcttgcgtcc cgccagttct gaaagggaat cggcgagcag cgttttatcg ctaagattaa
     6961 aatcgagcag gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa tagaactggc
     7021 ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc
     7081 tgcggctgcc gagcactttg ccctgacgaa tgaacaatac gtggacacaa gccatgcccg
     7141 catcgaacgc cacaccaata acgtcgaggt cgtcgccggt attggaaacg aattgttttt
     7201 cggtgacgcg tcgcaccgcc tgaatttggt cgcgaatacg tgcagcttct tcaaactcca
     7261 gattctggct ggcagtttcc atacgactaa tgagttgcgt aagcacctga tcatctttgc
     7321 cagacaaaaa caggcgcaca tactcaacct gctgagcgta ttcttcttca ctcaccagtc
     7381 cttcaacgca cggtcccaga cagcgcccta tctgatattg cagacacgga cgcgagcgat
     7441 tgcgataaac actattttcg cactggcgaa tggggaaaat cttttgcagt agcgccagtg
     7501 tttcacgtac ggcatagcca ttcgggaacg ggccgaaata ttcacctttg gcatgcttcg
     7561 caccacgatg catcgccaga cgcgggtggg tatcgccact caggaagata aaaggataag
     7621 atttatcatc gcgtagcaaa acgttgtaac gcggctgata gagtttgatg tagttgtgtt
     7681 ccagcaacag cgcttcggtt tctgtatgcg taaccgttac atcaatttgc tggatctggg
     7741 cgaccagcgc ttcggttttg cgcgaagcga ggttgctgcg gaaatagctg gaaagccgtt
     7801 ttttcaggtc tttcgctttg ccgacataga taaccgtacc accagcatcg tacatgcgat
     7861 aaacgcctgg ctggcaggtt acggttttta aaaacgcttt tgcgtcaaac tgatcactca
     7921 ctgacttgat aatgtctccg cattacacag accatggcga attgccaggt gagtcagctc
     7981 aacatcgcca tgaatgttta gtttactgaa catacgatag cggtagctgt tcaccgtttt
     8041 cggactgaga ttgagctgtt ctgagatctc attgaccttc tggcccttgg tgatcatcag
     8101 cataatctgc aattcacgtt cagacaaact ggcaaatggg ctttctgttt tttctggttc
     8161 gatctggctt aacgccattt gttgagcgat atcagaagca atgtaacgct gccctgaata
     8221 gacagaacga atcgcactca cgacttcctg cggagccgcg cctttgctga ggtagcccgc
     8281 agcaccggcc tgcatgactt tcgctggtaa agggttttct gtatggacgg taagcatgat
     8341 gattttgaca tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc
     8401 cggcatactc atgtccatta gcaccacgtc aacggcattt gtccggcacc acttaacggc
     8461 gtcttcaccg cacgatgcct caccgacgac ttttataccc tttatatctt ccagaatgcg
     8521 tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt agaacgttga tcaaaggaat
     8581 atctccagaa atagggataa cgctactgat agttagtcat tcgtatatta gcggttttta
     8641 ttgcaacttt gaaacgttaa aaatggtggg tattcgattt ttctctcgtt tttgtaaatt
     8701 cgattgtcca cagttatggt cacgcccgtc accaacggct tccgggatcg tttcacagta
     8761 agcccggcga tgaaatgtga caaaaattga ctttattcag caaaaatgaa aatcagcccg
     8821 ctgaatatgt aacattaatt aacccgaaca atacgggcaa aaaacattca tgataaaaat
     8881 atttattggt cattatatta acgcatttaa tagcattgtt gacgtcacgc tcaaaaaaca
     8941 accactgcta tttttagcaa agcttatggt atactccgcc gccttaacat ttttcaccgc
     9001 aaattttcat tgcaacatga cgaggaaaat aaatgagtac gcctgatttt tctactgccg
     9061 agaataatca agaactggca aatgaagtct cctgcctgaa agcgatgctg acgctgatgc
     9121 tacaggcgat gggacaagct gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg
     9181 cgctgatcga agacgaaacc caggctgcag tattttccaa aacggttaag caaattaaac
     9241 aagcctaccg tcagtaatat aaaaccggct gagagcgtgc ctttcagccg atttttgcat
     9301 ctggcacgca gaaagaaaag cgatcaaatt aagccagtag cggccgcgta acaggccacc
     9361 tgggtcttat ttggtgcatt gatttttttc tgcatgtttt tctgatggaa attgaccgta
     9421 ttctcagaga ttgacaaaat catcgctatc tctgctgatg tcttcccttc cgccgtccat
     9481 ttcagaattt ctttttcgcg cttgctgaaa ttcatctctg gcgtcatcac tatttcatca
     9541 tttaaacgca tcagagccat cagactttcg cgcaccagta actgcatttt taattgcagt
     9601 tcatcactaa gaatgggtat ttcgcgcgtg ctgcaacggg aaaaggacaa aaagcccagc
     9661 gcccgatttg gcagcattaa atactgagtg acaccgcggc gtaaaccatg tgcgtgcgcg
     9721 gcttcccata atggctgtgc ttcgctgaat aagtcatcat tccacattaa atggccctga
     9781 ctaaagtttt cagggttcag cacaggatca attgcgagaa aattttttgc ctgataataa
     9841 ctaacccacg actcagggta attggtgtag aaagccactt taggtcgcgt gaatggtacc
     9901 gggtggcgaa cacataacga atagtaatcg tactccagct gctgagcctg aagctctatt
     9961 tcatggtaga cctcttctgc ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc
    10021 cagctgaaaa aatccgtatc ctgcatagta aaccgcaacg cccctgagag tgagtatcat
    10081 ttataatgat aattcaaact atcacataaa taagatttat atataattta tattattcag
    10141 gcaatgaatt acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat
    10201 ttgttaagca gttttatcac gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg
    10261 gctgattttt tcttattatt gcagtagaaa cttctcgagg aactggcggg tgcgaggctg
    10321 ctggggatca gcaaataacg cttttgctgc cccctgctcg actatccgcc cctggtccat
    10381 aaaaatcgcc cggtccgcaa catcccgggc aaagctcatt tcgtgcgtca caatcaccat
    10441 cgtgcgcttt tcctgcgcca gctggcggat tgtgttcagg acttcgccca ccagctctgg
    10501 atccagcgct gacgttggct cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg
    10561 cgcaatcgct acacgctgct gttgaccgcc agacaaacga cgtggatagc tggtttcttt
    10621 accggccagc ccaactttcg ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg
    10681 ttcgcctttg acgatcaccg gcccttcaat aatgttttcc agcaccgtac gatgcggaaa
    10741 caaattaaag ttctggaaga caaacccgac gtgctgacgc aactggcgga tcagcgattt
    10801 ttgctgactt aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tcgttcccgc
    10861 ttccggttgt tccagcagat ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg
    10921 accgataatt gccaccactt cgccaggctt tacctcgagg tcgataccgt gcagcaccgt
    10981 ctgaccgtga aattttttca ccaggttctt aacttcaatg gcactcattt tggttctctc
    11041 tcctggcgat taagttgatt ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg
    11101 atccagtaga tgagcgaagc cgccaggtac atggtgaaca cctccagcgt gcgcgaggta
    11161 atcaactgcg cctgacggaa cagctccggc acctggattg tcgcggccag cgacgtatcc
    11221 tttaccaggc tgataaagct gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc
    11281 aaaatcgcgc gacgcatagt ctgccatggc gtcataccaa tactggcagc tgcttcccac
    11341 tgacctttat caatagaaga aatggcggcg cgcagtgttt cggcggcata ggcggcagtg
    11401 ttcagcgaca gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgcggc
    11461 aggccgtaat agatcataaa cagttgtgcg atgagcggtg taccacgaaa aatagagata
    11521 taaaaacgcg ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata
    11581 aacccgagca gtaagccaaa aaacatgccg ccgatactga gttgcagcgt atacccagcc
    11641 cctttcagca ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt
    11701 cccggagcta aacgtgggga tgataggcga acagcgcagg cgcaccgccg gtatgaataa
    11761 acagaatcgg cccttcatct ttgaagcgtt tctgactgat acagccaatc agccccgcca
    11821 tcgcttttcc ggtatacaca ggatcaagca gaatgccttc cagccgcgcc agcaatttca
    11881 ctgcttccat gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga
    11941 gtataatttc cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa
    12001 ccactttcgg caactgatcg gcaacggaac gcgacacggt cacgccaatc agttcgcttt
    12061 caggcatcag gtgttccagc ccaacagcca gtccggcgtg agttccggca ctgcccgatg
    12121 cgactaccac cgacgaaata ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac
    12181 tctccacata acctagcgcg ccaagagcat tagaaccgcc aaccggaatg acatacgggc
    12241 gaaagccttg tgcttcgact cgcgtcgcca gctcttccag ttgggcattg ggatcggtca
    12301 gtgcttcgca catttcaatc tgggtattga acagatccag caacaagcga ttgccgttgg
    12361 ttaaatagtt ttctgcggtt gtgccaatag gattttccag cagcgccaca cagtgcagac
    12421 cgagtttcgc cgcaactgcg gcagtctgcc gcacatggtt agactggatc accccggcag
    12481 taatcagcgt atcggcacct tcgcgcagag catctgccgc gagaaattct agcttacgta
    12541 atttattgcc gcccattgcc atgggggtga cgtcatcccg tttgatgaaa atttcccgtc
    12601 ctagataatc agaaaagcgc ggcagatatt cgagcggcgt tggcgcgccg ataaactcca
    12661 accgtggaaa acgggttaaa ttatgcagtg gcataacagc ctccgatgta tgttgttgtg
    12721 attttcttat tatgcacgct gaaaacgcgt aaataaaaaa gg
//
LOCUS       DAZOXR010000122        12731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZOXR010000122 DAZOXR010000000
VERSION     DAZOXR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.124"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUNN_002963"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUNN_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0577276.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUNN_002964"
     CDS             433..621
                     /locus_tag="ACHUNN_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0577277.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUNN_002965"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUNN_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0577278.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUNN_002966"
     CDS             816..989
                     /locus_tag="ACHUNN_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0577279.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUNN_002967"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUNN_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUNN_002968"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUNN_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR0577280.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUNN_002969"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUNN_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0577281.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUNN_002970"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUNN_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0577282.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUNN_002971"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUNN_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0577283.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUNN_002972"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUNN_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUNN_002973"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUNN_002973"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0577284.1"
                     /translation="MFSRELDDEQLTQIASAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUNN_002974"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUNN_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0577285.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUNN_002975"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUNN_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0577286.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..>12731
                     /locus_tag="ACHUNN_002976"
     CDS             12531..>12731
                     /locus_tag="ACHUNN_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577287.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECE"
BASE COUNT         3104 a         3236 c         3315 g         3076 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga a
//
LOCUS       DAZOXR010000123        12538 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZOXR010000123 DAZOXR010000000
VERSION     DAZOXR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.125"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            13..2202
                     /gene="fhuA"
                     /locus_tag="ACHUNN_002977"
     CDS             13..2202
                     /gene="fhuA"
                     /locus_tag="ACHUNN_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0577288.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            2253..3050
                     /gene="fhuC"
                     /locus_tag="ACHUNN_002978"
     CDS             2253..3050
                     /gene="fhuC"
                     /locus_tag="ACHUNN_002978"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0577289.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            3050..3940
                     /gene="fhuD"
                     /locus_tag="ACHUNN_002979"
     CDS             3050..3940
                     /gene="fhuD"
                     /locus_tag="ACHUNN_002979"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0577290.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            3937..5919
                     /gene="fhuB"
                     /locus_tag="ACHUNN_002980"
     CDS             3937..5919
                     /gene="fhuB"
                     /locus_tag="ACHUNN_002980"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0577291.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(6077..7357)
                     /gene="hemL"
                     /locus_tag="ACHUNN_002981"
     CDS             complement(6077..7357)
                     /gene="hemL"
                     /locus_tag="ACHUNN_002981"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0577292.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            7523..7570
                     /gene="yadX"
                     /locus_tag="ACHUNN_002982"
     CDS             7523..7570
                     /gene="yadX"
                     /locus_tag="ACHUNN_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0577293.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            7582..9003
                     /gene="clcA"
                     /locus_tag="ACHUNN_002983"
     CDS             7582..9003
                     /gene="clcA"
                     /locus_tag="ACHUNN_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0577294.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            9027..9092
                     /gene="yadW"
                     /locus_tag="ACHUNN_002984"
     CDS             9027..9092
                     /gene="yadW"
                     /locus_tag="ACHUNN_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0577295.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            9085..9429
                     /gene="erpA"
                     /locus_tag="ACHUNN_002985"
     CDS             9085..9429
                     /gene="erpA"
                     /locus_tag="ACHUNN_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0577296.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(9476..10099)
                     /locus_tag="ACHUNN_002986"
     CDS             complement(9476..10099)
                     /locus_tag="ACHUNN_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0577297.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(10137..10937)
                     /gene="btuF"
                     /locus_tag="ACHUNN_002987"
     CDS             complement(10137..10937)
                     /gene="btuF"
                     /locus_tag="ACHUNN_002987"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0577298.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(10930..11628)
                     /gene="mtnN"
                     /locus_tag="ACHUNN_002988"
     CDS             complement(10930..11628)
                     /gene="mtnN"
                     /locus_tag="ACHUNN_002988"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0577299.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            11712..>12538
                     /gene="dgt"
                     /locus_tag="ACHUNN_002989"
     CDS             11712..>12538
                     /gene="dgt"
                     /locus_tag="ACHUNN_002989"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0577300.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         2796 a         3153 c         3477 g         3112 t
ORIGIN      
        1 agagatatac caatggcgcg ttccaaaact gctcagccaa aacactcact gcgtaaaatc
       61 gcagttgtag tagccacagc ggttagcggc atgtctgttt atgcacaggc agcggttgaa
      121 ccgaaagaag acactatcac cgttaccgct gcacctgcgc cgcaagaaag cgcatggggg
      181 cctgctgcaa ctattgcggc gaagcactct gctactgcga ctaaaacgga tacaccaatt
      241 gaaaaaacgc cgcagtctat ttcggttgtg acaaatgaag agatgcagat gcgtcaattt
      301 cagtctgtaa aagaagcatt aggttataca cctggcgtta cggttagcag tcgtggtgct
      361 tctaatacat atgattttgt tatcattcgt ggtttctcat ctgttggtct gaatcaaaat
      421 aattaccttg atggactaaa acttcagggc aacttttata acgatgctgt gattgatcct
      481 tacatgctcg agagggttga actgatgcgt gggccgacgt ctgttctcta tggaaaaagt
      541 aatcctggtg ggattatttc tatggtgagt aagcgtccga ccactgaacc cctgaaagaa
      601 attcagttta aaatggggac ggataatctg tttcagaccg gattcgattt cagtgatgca
      661 ctggatgata acggtgagtt ctcttaccgt ttgacgggcc tcgctcgctc aacaaacgaa
      721 cagcagaaaa actctgaatc tcagcgttat accattgctc catcattctc atggcgtcca
      781 gacgacaaaa ccaattttac tttcctgtcc tatttccaga atgaaccaga aacgggttat
      841 tacggttggt tgccgaaaga ggggaccgtt gagccattgc ctaatggtaa gcgtctaccg
      901 actgacttca atgaaggtgc atcgaataat acatactccc gtaaccagaa aatggtggga
      961 tatagttttg aacatggttt taatgacacc ttcaccgtgc gtcagaatct gcgtttcagt
     1021 gaaatgaaaa cctcacagaa aagtgtttat ggcacaggga ttgctggtga tggtcatacc
     1081 ctaaaccgcg ggacagtggt ggataatgag cgtctacaaa actttagcgt tgatacccaa
     1141 cttgaaagta agtttgctac aggtgacgtc gagcatactt tgctgacagg ggtagacttc
     1201 atgcggatgc gtaatgatat caatgccagc tttggatccg caccatccat cgatctttat
     1261 aacaaatatc accctgaata ctttgcattt ggtagcgcag agccatacca gatgaatgaa
     1321 agcaaacaaa caggtattta tgttcaggat caggcggaat ggaataaatg ggtattcact
     1381 ctggggggac gctacgattg gtctaagcaa gcgaccactg ttcgtgaaaa ctcttatacg
     1441 ccgactgaag gttatattga gcgcaatgat catcagttca cctggcgcgg tggtgtaaat
     1501 tacttattcg ataatggtat ttcaccttac tttagctata gccagtcctt tgaaccgagt
     1561 gctttcgatc tgtggagcaa cccgcgcatt tcctataagc catcgaaagg tgaacagtat
     1621 gaagctggcg taaaatatgt tccgaatgat atgccggtcg ttgttacggg cgcagtctat
     1681 caattgacga aaacaaataa cctgacagca gacccaacaa acccattagc gcaagtccca
     1741 gcaggtgaga ttcgcgctcg tggtgtggaa cttgaagcaa aagcggcgtt aaatgccaat
     1801 attaacttga cggcttctta tacctacacc gatgcggaat acaccaaaga caccaatctc
     1861 aaaggtaaaa ctccagaaca agtaccggag catatggcat ctctctgggg ggattatacc
     1921 ttcaatgaag ggccgctttc tggtttaaca ttgggaacag gtggtcgttt tattggttcc
     1981 agctatggtg atccggcaaa cacttttaaa gtgggtagca cagctgtaat ggatgctgtt
     2041 gtaaaatatg atctggcacg ctttggtatg gcgggatcca gccttgctgt gaacgtcaac
     2101 aatttgctcg atcgtgagta tgttgccagt tgcttccaga cttatggctg cttctggggc
     2161 gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct aatttctctt ttgggacacg
     2221 gatttccgtg cccatttcac aagttggctg ttatgcagga atacacgaat cattccgata
     2281 ccacttttgc actgcgtaat atctcctttc gtgtgcccgg gcgcacgctt ttgcatccgc
     2341 tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat tggtcacaac ggttctggta
     2401 aatccactct gctcaaaatg cttggtcgtc atcagcctcc gtcagaaggg gagattcttc
     2461 ttgatgccca gccgctggaa agctggagca gcaaagcgtt tgcccgcaaa gtggcttatt
     2521 tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg tgaactggtg gcgattggtc
     2581 gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc agatcgcgaa aaggtcgagg
     2641 aagctatctc gctggttggc ttaaaaccgc tggcgcatcg gctggtcgat agtctctctg
     2701 gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc gcaggatagc cgttgtctgt
     2761 tgctcgacga accgacttcg gcgctggata tcgcccacca ggttgatgtg ctggcgctgg
     2821 tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc cgtgttgcac gatatcaata
     2881 tggcggcacg ctactgtgat tatctggtcg ccctgcgcag tggtgaaatg attgctcagg
     2941 gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat gatttatggc atcccgatgg
     3001 gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt tgtttattga tgagcggctt
     3061 acctcttatt tcgcgccgtc gactgttaac ggcgatggcg ctttctccgt tgttatggca
     3121 gatgaatacc gcccacgcgg cggctattga ccccaatcgt attgtggcgc tggagtggtt
     3181 gccggtggaa ttactgctgg cgctcggcat cgtgccttac ggcgtggcgg ataccatcaa
     3241 ctatcgcctg tgggtcagcg aaccaccatt gccggactca gtgattgacg tcggtttgcg
     3301 tacagaacct aaccttgaac tgctgaccga aatgaaaccg tcgtttatgg tctggtcggc
     3361 aggatatggc ccttcatcag aaatgctggc acgtattgcg ccgggtcgcg gatttaactt
     3421 cagtgacggc aaacagccgt tggcgatggc gcgtaaatcg ctgacggaaa tggcagattt
     3481 acttaacctg caaagcgcag cggaaacgca tttagcgcaa tacgaagact ttatccgcag
     3541 catgaaaccc cgctttgtga agcgtggtgc acgcccgtta ctgctgacga cgcttatcga
     3601 tccgcgccat atgctggtct tcggtccaaa cagcttgttc caggaaattc tcgatgagta
     3661 cggcatccca aatgcctggc aaggggaaac caacttctgg ggcagtaccg ccgtcagtat
     3721 cgatcgtctg gcagcgtata aagacgttga tgtgctctgt tttgatcacg acaacagcaa
     3781 agacatggat gcgctaatgg caaccccgct gtggcaggcc atgccgtttg tccgcgccgg
     3841 acgctttcag cgcgtacctg cagtctggtt ttatggtgcg acactctcgg caatgcactt
     3901 tgtgcgcgtt ctggataacg ccatcggagg taaagcgtga gtaaacgaat tgcgcttttt
     3961 ccggcgttat tgctggcgct gttagtgatt gtcgctacgg cgctcacctg gatgaacttc
     4021 tcgcaggcgc tgccgcgtag ccagtgggcg caggctgcct ggtcgccaga catcgatgtc
     4081 atcgagcaga tgatttttca ctacagcttg ttgccgcgtc tggcgatttc gctgctggtg
     4141 ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag tgctgcgtaa cccgctggcg
     4201 gagccgacga cgcttggcgt tgctacaggc gcgcaactgg gaattaccgt caccacactt
     4261 tgggcgatcc caggtgctat ggcgagccag tttgccgcac tggtgggcgc ttgtgtcgtt
     4321 ggcttaatcg tctttggcgt cgcgtggggg aaacggcttt cgccggttac gcttattctc
     4381 gcggggctgg tggtgagcct ttattgcggg gcaattaatc agctactggt tatcttccat
     4441 cacgaccagc tgcaaagcat gttcctgtgg agcaccggaa cgctgacgca aaccgactgg
     4501 ggcggcgttg agcgtttatg gccgcagttg ctgggcggcg tgatgttgac gttattgcta
     4561 cttcgcccgt taaccctgat ggggcttgat gatggcgtgg cgcgcaatct cgggctggcc
     4621 ttgtcgctcg cgcgtctggc ggcgctgtcg ctggcgatag tcatcagtgc gctgctggtg
     4681 aatgcggtgg ggattatcgg ctttatcggt ttgttcgcgc cgctgctggc gaaaatgctg
     4741 ggggcgcggc gtttgttgcc acggttgatg ctggcgtcgc tgattggggc gctgatcctc
     4801 tggctttccg atcaaatcat cctctggctg actcgcgtgt ggatggaagt gtccaccggt
     4861 tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc tgttgccgcg tttacgcagc
     4921 attagcgcgc cggatatgaa ggtcaacgat cgtgtcgcgg ctgaacgcca acatgtgctg
     4981 gcgtttgccc tcgcgggcgg cgtgctgctg ttgatggctg tggtggtggc gctgtcgttt
     5041 ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt tgctcgagga tttgatgccc
     5101 tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg tcatgctggc ggtggcgggc
     5161 tgtattattc agcggctgac cggaaacccg atggcaagcc cggaagtgct ggggattagc
     5221 tctggcgcgg cgtttggcgt ggtgttaatg ctgtttctgg tgccgggtaa tgcctttggc
     5281 tggctgttac ccgcgggcag tatcggggcg gcggtgacgc tgttgatcat tatgatcgcc
     5341 gccggccgcg gtggattttc cccacaccgt atgttactgg cggggatggc gttaagtacc
     5401 gcattcacca tgcttttgat gatgttgcag gcgagcggtg acccgcgaat ggcgcaagtg
     5461 ctgacctgga tttccggttc gacctacaac gcgaccgatg cgcaggtctg gcgtaccgga
     5521 attgtgatgg tgattttgct ggcgattacc ccgctgtacc gccgctggct gaccatttta
     5581 ccgctgggtg gtgataccgc acgagccgta ggaatggcgc tgacgccgac gcgaattgcg
     5641 ctgctgctgt tagcggcttg cctgacggcg accgcgacga tgactattgg accgttgagt
     5701 tttgttggtt taatggcacc gcatattgcg cggatgatgg gctttcgacg gacgatgcca
     5761 cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg tattcgctga ctggtgtggg
     5821 cggatggtgc tgtttccatt ccagatcccg gcggggctgc tgtcgacctt tatcggcgcg
     5881 ccatatttta tctatttgct gagaaagcag agccgttaat ttttccatat aacggccctg
     5941 tacgcctgga cggataaagc gtttacgcga caacggatgc ccgatgcgac gctggcgcgt
     6001 cttatcgggc ctacaaacgg ccccgaatcg taggtcggat aaggcgttca cgccgcatcc
     6061 gacaaaccat gctggatcac aacttcgcaa acacccgacg tgcagcatcg atggtgttat
     6121 tgatatcttc catgctgtgc gccacggaca taaagcccgc ttcaaacgct gacggtgcca
     6181 ggtaaacacc ttcgtccagc atcatatgga agaaacgctt aaagcgttcc acgtcacagg
     6241 ccatcacatc ctgatagcac gtcacggact cggcgtcggt aaagaaaata ccgaacatgc
     6301 cgccaacgtg gttaacgacc agcggaattc cggcttcttc tgccgcttcc agcagacctt
     6361 ctgccagacg tgttgtcagc tcatccaacg tttcgtgaac gcccggttgc gcgacttcat
     6421 tcagacaggc gaaaccggct gccatcgcaa ttgggttacc ggaaagcgta cccgcctgat
     6481 agaccggacc cgtcggggcc agcgcatcca ttacatcacg acgaccaccg aatgcgccta
     6541 ccggcattcc accgccgatg attttgccca ggcaggtgag atccggttcc acaccgtaat
     6601 aatcctgtgc gccagccagt gccacgcgga agccggtcat tacttcatcg atgatcagca
     6661 atgcgccaaa ttcgtcgcac agcgcacgca gacctggcag gaagtctggc agcggtggaa
     6721 cacagttcat attgcctgcc accggctcga tgataataca ggcaatctct tgcgggtatt
     6781 gctcaaacgc ggcgcgtaca gaagccagat cgttataagt acaggttaag gtatgtttgg
     6841 cgaaatctgc cggaacgccc ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg
     6901 ctttcaccag caggcagtca gcgtgaccgt ggtaacaccc ttcaaattta atgattttgt
     6961 cgcgaccggt aaaaccacgg gccagacgga tggcgctcat cgtcgcctcg gtgccggagt
     7021 tcaccatgcg caccatatcc atggtcggga ccaattcggt caccagttgc gccattttca
     7081 cttccatttc ggttggtgca ccaaagctta aaccacgctc ggcggcttca atcacggcat
     7141 tgcggattgc cggatggtta tggcccagca ccatcggccc ccaggaaccg acataatcga
     7201 tataggcttt gccatcaaca tcgtacagat aagcgccgtc cgctttttcg ataaacagtg
     7261 gagtgccgcc cacgccagta aaggcgcgaa caggggagtt cacaccgcca gggatcagct
     7321 cgcgcgctgc gctgtaaaga ttttcagact tactcatgga gggttcctga ttcgtagaaa
     7381 aagtgaatgg ctgctattct atgttattca taacaagtta aatactcgtc aaacatcagg
     7441 ctgcttgtac tggtgcaatc ctgatttctt agagtataaa agttttgtgc atttgaaaca
     7501 ttacgctttg caaaggattt tcatggaacg tgcgagtaaa atgccgtcat cttatttgta
     7561 tgaccaataa gtgatcattg gatgaaaact gatactccct ctttagaaac accgcaggcc
     7621 gcgcgcctgc gacgcagaca actgattcgc caacttcttg agcgcgataa aaccccgtta
     7681 gccattttgt ttatggcggc agtcgtcggc acgcttgttg ggctggcagc ggttgctttt
     7741 gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca tactgctgat
     7801 aattatccgc ttctgttaac cgtcgctttt ctctgttcgg cggtgctggc gatgtttggc
     7861 tactttttgg tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa
     7921 ggggcgctgg aagatcaacg tcccgttcgc tggtggcgtg tattgccggt gaagttcttt
     7981 ggcgggctgg ggacactcgg cggaggcatg gtgttggggc gcgaagggcc aaccgtgcag
     8041 atcggcggta acattggccg tatggtgctt gatattttcc gcctgaaagg tgacgaagct
     8101 cgccatacgc tgctggcaac cggtgctgct gcggggctgg ctgcggcctt taacgcgccg
     8161 ctggcgggta ttttgtttat tatcgaagag atgcgtccgc agtttcgcta tacgttaatt
     8221 tcgataaaag cggtatttat tggcgtcatt atgtcgacca ttatgtaccg gatttttaat
     8281 catgaagttg cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg
     8341 ctttatctga tcctcggtat tatttttggc attttcggcc ctatttttaa taaatgggtg
     8401 ctggggatgc aggatttgct gcaccgtgtg cacggcggca atattaccaa atgggtactg
     8461 atgggcggtg cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg
     8521 ggcggcggtt ttaacctgat tcctatcgct accgcgggga atttcagcat gggaatgctg
     8581 gtgtttatct tcgtcgctcg ggtcattacc accttactct gcttctcttc cggcgcgccg
     8641 ggcggtattt ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg
     8701 gttgccgttg agctgtttcc gcaatatcac cttgaggcgg ggacgtttgc tattgccgga
     8761 atgggggcat tactggcggc atctattcgc gcgccgttaa cggggatcat tctggttctg
     8821 gagatgaccg ataactacca gctcattttg ccaatgatta ttaccggtct tggcgcaaca
     8881 ctattagcgc aatttaccgg cgggaaaccg ctatactcgg cgattcttgc gcgcacgctg
     8941 gcaaaacagg aagctgagca actggcgcga agcaaggccg catcagccag cgagaatact
     9001 tgaacgaaat accagggtat tagataatgg cgattattat tgggttagaa tttgcccaat
     9061 tgccgatgtc gtttggagca aaatatgagt gatgacgtag cactgccgct ggagtttacc
     9121 gacgcagcag ccaacaaagt taaaagcctg atcgctgacg aagataaccc gaatctgaaa
     9181 ttacgcgtgt atatcaccgg tggcggttgc agcggcttcc agtatggttt cacctttgat
     9241 gatcaggtga acgaaggcga tatgaccatc gaaaaacagg gcgttggctt ggtggttgat
     9301 ccgatgagcc tgcaatatct ggtcggcggt tccgttgact ataccgaagg actggaaggt
     9361 tctcgtttca tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt
     9421 agcatctaat ctgttgttca tgtgccggat aaactatccg gcgcatcttc tctcttcaac
     9481 gcccattctc atccagcgca aacgtcggta gcttaagatg ccaacgaata gccgccagcc
     9541 gaatcaatag cgtcacgacc atgcccatca tactggctgt ttccagtggt acggaaaatg
     9601 tgtaataagc cgtagcgtgg acaataccgc cgataataca ggcagttgcg tagatttctg
     9661 tacgtaaaat catggggatc tcgcgggcca gtacatcacg aatgatcccg ccgccaacgc
     9721 cagtaatgac gaccatacaa accgcgatta acggaccgac ttccgcatta aaggctttat
     9781 tcacgccaat gccgacaaac accgccagac caacggcgtc cagcaccggc aacatccatt
     9841 ttggtaagcg tcttggctgg cgcaccagca cgatggtcag catgctggtt accattgcaa
     9901 cgaccagatc ggtgggatct ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc
     9961 gaattgtccc gccgcctaca gcggtaacca cgcccagtac cagaacacca aaagggtcca
    10021 tacgcaattt tccggctaac aaaacgccgg agatggcaaa taccgctgtg ccgactatat
    10081 ccagccaata gacgagcatt gttcaatccc cactgagcac ctaaaaaggt caggcgctaa
    10141 tctacctgtg aaagcgcatt acagagctgt tgtgcagcga ggataatacg tgggcttgca
    10201 cgttcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc accccagtat
    10261 tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg tggcgagcgt
    10321 gctaacacct gttcgcggct aacttgcggc cagggaaccc ggctgtcttt aaagatgttt
    10381 tctccgccac aaacttcgag tacctggttt tgaatcgact cttttccact ggtaaatggc
    10441 ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata ttgcgctttc
    10501 aattgcgcgt actgatccag cagggattgc gcggcttgtt cggccttatc cggttgcgga
    10561 ctccaggggg ccaattgacg taacgcattg gcaatttgtt caatgcttgt cgcatcgacc
    10621 cacatcactt ttattcccag cgaagccagc tggtcaacct gccgctcggc attacctcca
    10681 cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc gttccagatt catcccctgc
    10741 caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata gctgctgacc
    10801 ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg
    10861 acgcgcggcg cggcgttgag ccacagtggc gcaagaaaag acagggcgac cagcgccctg
    10921 aacagtgact tagccatgtg ccagtttctg caccagtgac tcaaccatca ggctggactg
    10981 tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat cggccacgtc
    11041 ggagatggcg cgtacgacaa caaacgggac gttgaaattg tggcagacat gggcgattgc
    11101 cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt tcgccagacc
    11161 aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag cgttaagatt
    11221 cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt taaagcctgc
    11281 cggacagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg cgtcgtgata
    11341 acgcgcttcg tccgagacaa cgatatcgcc cactttcagc gttggtgcca ggccaccggc
    11401 agaaccggtg ttaataatca catctggctt gcagtgttcc aacagcagag tggcacccag
    11461 cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc cattcagttg
    11521 gccggtatag atttcgcaac cgccgagact gatagtttga cggttttcga ttttgtcacg
    11581 cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag atttactcgc
    11641 gataagcccg atttgaaagc atagtttacc atgcgcttac ggggaagcgt atttctcacg
    11701 cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat cgtcgttacc
    11761 gttcaccgca gggcgttaaa accgaacatg agatcctgcg gatcttcgag agcgatcgcg
    11821 ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacccag gtttttccac
    11881 tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg
    11941 gacgctacat cgccaaagaa attttaagcc gtctgaaaga gcttaaattg ctggaagcat
    12001 acggcctgga tgaactgacc ggcccctttg aaagcattgt tgagatgtca tgcctgatgc
    12061 acgatatcgg caatccgccg tttggtcatt ttggcgaagc agcgataaat gactggtttc
    12121 gccaacgttt gcacccggaa gatgccgaaa gccagcctct gactgacgat cgctgcagcg
    12181 tggcggcact acgtttacgg gacggggaag aacagcttaa cgagctgcgg cgcaagattc
    12241 gtcaggactt atgtcatttt gaggggaatg cacaaggcat tcgtctggtg catacattga
    12301 tgcggatgaa tctcacttgg gcacaggttg gcggtatttt aaaatatacc cgtccggcgt
    12361 ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg ggttattatc
    12421 tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg ctttacagtc
    12481 gttttccatt aacgtggatt atggaagctg ccgacgacat ctcctattgt gtggcaga
//
LOCUS       DAZOXR010000124        12359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZOXR010000124 DAZOXR010000000
VERSION     DAZOXR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.126"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHUNN_002990"
     CDS             complement(<1..330)
                     /locus_tag="ACHUNN_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR0577301.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUNN_002991"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUNN_002991"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0577302.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUNN_002992"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUNN_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0577303.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUNN_002993"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUNN_002993"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0577304.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUNN_002994"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUNN_002994"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0577305.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUNN_002995"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUNN_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0577306.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUNN_002996"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUNN_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0577307.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUNN_002997"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUNN_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0577308.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUNN_002998"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUNN_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0577309.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUNN_002999"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUNN_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0577310.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUNN_003000"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUNN_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0577311.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUNN_003001"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUNN_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0577312.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUNN_003002"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUNN_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0577313.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAZOXR010000125        12088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZOXR010000125 DAZOXR010000000
VERSION     DAZOXR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.127"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            245..1354
                     /gene="livK"
                     /locus_tag="ACHUNN_003003"
     CDS             245..1354
                     /gene="livK"
                     /locus_tag="ACHUNN_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0577314.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1402..2328
                     /gene="livH"
                     /locus_tag="ACHUNN_003004"
     CDS             1402..2328
                     /gene="livH"
                     /locus_tag="ACHUNN_003004"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0577315.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2325..3602
                     /gene="livM"
                     /locus_tag="ACHUNN_003005"
     CDS             2325..3602
                     /gene="livM"
                     /locus_tag="ACHUNN_003005"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0577316.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3599..4366
                     /gene="livG"
                     /locus_tag="ACHUNN_003006"
     CDS             3599..4366
                     /gene="livG"
                     /locus_tag="ACHUNN_003006"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0577317.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4368..5081
                     /gene="livF"
                     /locus_tag="ACHUNN_003007"
     CDS             4368..5081
                     /gene="livF"
                     /locus_tag="ACHUNN_003007"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0577318.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5365..6429
                     /gene="ugpB"
                     /locus_tag="ACHUNN_003008"
                     /pseudo
     CDS             5365..6429
                     /gene="ugpB"
                     /locus_tag="ACHUNN_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            6527..7414
                     /gene="ugpA"
                     /locus_tag="ACHUNN_003009"
     CDS             6527..7414
                     /gene="ugpA"
                     /locus_tag="ACHUNN_003009"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0577319.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7411..8256
                     /gene="ugpE"
                     /locus_tag="ACHUNN_003010"
     CDS             7411..8256
                     /gene="ugpE"
                     /locus_tag="ACHUNN_003010"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0577320.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8258..9328
                     /gene="ugpC"
                     /locus_tag="ACHUNN_003011"
     CDS             8258..9328
                     /gene="ugpC"
                     /locus_tag="ACHUNN_003011"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR0577321.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9325..10068
                     /gene="ugpQ"
                     /locus_tag="ACHUNN_003012"
     CDS             9325..10068
                     /gene="ugpQ"
                     /locus_tag="ACHUNN_003012"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0577322.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10055..10495)
                     /locus_tag="ACHUNN_003013"
     CDS             complement(10055..10495)
                     /locus_tag="ACHUNN_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0577323.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10615..>12088
                     /gene="ggt"
                     /locus_tag="ACHUNN_003014"
     CDS             10615..>12088
                     /gene="ggt"
                     /locus_tag="ACHUNN_003014"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0577324.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         2630 a         3103 c         3477 g         2878 t
ORIGIN      
        1 cgccctttgt gccgttattt tatgctgaca aaggcacttt tttctgttta tctatcaata
       61 aattcagaat attatctgtt cttaatcgac tgaaaaatag ggattttaat cactgttatc
      121 acaaaatact gcgctaaacc cttaatcaga caggcaaaaa cagtgcagta taaaaaaaga
      181 acagtctgat ttgttaacac ataaaaacaa agcaacacaa catcacgaat ggggattttt
      241 gactatgaaa cggaatgcga aaactatcat cgcagggatg attgcactgg caatttcaca
      301 caccgctatg gctgacgata ttaaagtcgc cgttgtcggc gcgatgtccg gcccgattgc
      361 ccagtggggc gatatggaat ttaacggcgc gcgtcaggcg attaaagaca ttaatgccaa
      421 agggggaatt aagggcgaca agctggttgg cgtggaatat gacgacgcct gcgacccgaa
      481 acaagccgtt gcggtcgcca acaaaatcgt taatgacggc attaaatacg ttattggtca
      541 tctgtgttct tcttctaccc aacctgcatc agatatctac gaagacgaag gtattctgat
      601 gatctcgccg ggagcgacca acccggagct gacccaacgc ggttatcaac acattatgcg
      661 tactgccggg ctggactctt cccaggggcc aacggcggca aaatacattc ttgagacggt
      721 gaagccccag cgcatcgcca tcattcacga caaacaacag tatggcgaag ggctggcgcg
      781 ttcggtgcag gacgggctga aagcggctaa cgccaacgtc gtcttcttcg acggtattac
      841 cgccggggag aaagatttct ccgcgctgat cgcccgcctg aaaaaagaaa acatcgactt
      901 cgtttactac ggcggttact acccggaaat ggggcagatg ctgcgccagg cccgttccgt
      961 tggcctgaaa actcagttta tggggccgga aggtgtgggt aacgcatcat tgtcgaatat
     1021 tgctggcgat gctgccgaag gcatgttggt cactatgcca aaacgctatg accaggatcc
     1081 ggcaaaccag ggcatcgttg atgcgctgaa agcagacaag aaagatccgt ccgggcctta
     1141 tgtctggatc acctacgcgg cggtgcaatc tctggcgact gcccttgagc gtactggcag
     1201 cgatgagccg ctggcgctgg tgaaagattt aaaagctaac ggtgcaaaca ccgtgattgg
     1261 gccgctgaac tgggatgaaa aaggcgatct taagggattt gattttggtg tcttccagtg
     1321 gcacgccgat ggttcatcca cggcagccaa gtgatcatcc caccgcccgt gaaatgcggg
     1381 cgggtttaga aaggttacct tatgtctgag cagtttttat atttcttgca gcagatgttt
     1441 aacggcgtca cgctgggcag tacctacgcg ctgatagcca tcggctacac catggtttac
     1501 ggcattatcg gcatgatcaa cttcgcccac ggcgaggttt acatgattgg tagctatgtc
     1561 tcctttatga tcatcgccgc actgatgatg atgggcattg ataccggctg gctgctggtg
     1621 gcggcgggat tcgtcggcgc aatcgtcatt gccagcgcct acggctggag tatcgaacgg
     1681 gtagcctatc gcccggtgcg taactctaag cgcctgattg cgctcatctc ggcaatcggc
     1741 atgtccatct tcctgcaaaa ctacgttagc ctgaccgaag gttcgcgtga cgtggcgctg
     1801 cccagtctgt ttaacggtca gtgggtggtg gggcatagcg aaaacttctc tgcctctatt
     1861 accaccatgc aggcagtgat ctggattgtt actttcctcg ccatgctggc gctgacgatt
     1921 ttcattcgtt attcccgtat ggggcgcgcc tgccgtgcct gcgcggaaga tctgaaaatg
     1981 gcgagcctgc ttggcatcaa caccgaccgg gtgattgctt tgacctttgt tattggcgcg
     2041 gcgatggcgg cagtggctgg cgtactgctc ggtcagttct acggcgtcat taacccctat
     2101 atcggcttta tggccgggat gaaagccttt accgctgcgg tgctcggtgg gattggcagc
     2161 attccagggg cgatgattgg cgggctgatc ctggggattg cggaggcgct ctcttctgcc
     2221 tatctgagta cggaatataa agatgtggtc tcattcgccc tgctgattct ggtgctgctg
     2281 gtgatgccga ccggtattct gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata
     2341 ttgcaatggc actgctctct gccgcgatgt tctttgtcct cgctggcgtc tttatgggcg
     2401 tgcaactgga gctggatggc accaaactgg tggtcgacag cgcttcggat gtccgttggc
     2461 agtgggtgtt tatcggcacg gcggtggtct ttttcttcca gcttttgcga cctgctttcc
     2521 agaaagggct aaaaagcgtt tccgggccga agtttattct gcccgcgatt gatggctcca
     2581 cggtgaagca gaaactgttc ctcgtggcgc tgctggtgct tgcggtgtcg tggccgttta
     2641 tggtgtcacg tgggacggtg gatatcgcca ctctgaccat gatctacatt atcctcggtc
     2701 tcgggctgaa tgtggttgtt ggtctttctg gtctgctggt gctggggtac ggcggttttt
     2761 acgccatcgg cgcttacact tttgcgctgc tcaatcacta ttacggtctg gggttctgga
     2821 cctgtttgcc gattgccgga ctgatggcag cggcggcggg cttcctgctt ggttttccgg
     2881 tgttgcgtct gcgcggtgat tatctggcga tcgttaccct cggtttcggc gaaattgtgc
     2941 gcatattgct gctcaataac accgaaatta ccggcggccc gaacggtatc agtcagatcc
     3001 cgaaaccgac tttcttcggc cttgagttca gccgtaccgc ccgtgaaggc ggttgggaca
     3061 cgttcagtaa tttctttggc ctgaaatacg atccctccga tcgcgtcatc ttcctgtatc
     3121 tggtggcgtt gctgctggtg gtgctaagcc tgtttgtcat taaccgcctg ctgcggatgc
     3181 cgctggggcg tgcgtgggaa gcgttgcgtg aagatgagat cgcctgccgt tcgctgggct
     3241 taagcccgcg tcgtatcaag ctgactgcct ttaccatcag tgccgcgttt gccggttttg
     3301 ccggaacgct gtttgctgcg cgccagggct ttgtcagccc ggaatccttc acctttgctg
     3361 aatcggcgtt tgtactggcg atagtggtgc tcggcggtat gggatcgcag tttgcggtga
     3421 ttctggcggc aattttgctg gtggtatcgc gcgaattgat gcgtgatttc aacgaataca
     3481 gcatgttaat gctcggtggt ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc
     3541 tgcccatgac gcgcccgcaa ctgaagctga aaaacggcgc agcgaaagga gagcaggcat
     3601 gagtcagcca ttattatctg tgaacggcct gatgatgcgc ttcggcggcc tgctggcggt
     3661 gaacaacgtc aatcttgaac tgtacccgca ggagatcgtc tcgttaatcg gccctaacgg
     3721 tgccggaaaa accacggttt ttaactgtct gaccggattc tacaaaccca ccggcggcac
     3781 cattttactg cgcaatcagc acctggaagg tttaccgggg cagcaaattg cccgcatggg
     3841 cgtggtgcgc accttccagc atgtgcgtct gttccgtgaa atgacggtaa ttgaaaacct
     3901 gctggtggcg cagcatcaac aactgaaaac cgggctgttc tctggcctgt tgaaaacgcc
     3961 ttctttccgt cgcgcccaga gcgaagcgct cgaccgcgcc gccacctggc ttgagcgcat
     4021 tggtttgctg gaacacgcca accgtcaggc gagtaacctg gcctatggtg accaacgccg
     4081 tctggagatt gcccgctgca tggtgacgca gccggagatt ttaatgctcg acgaaccggc
     4141 ggcaggtctt aacccaaaag aaaccaaaga gctggatgag ctgattgccg aactgcgcaa
     4201 tcatcacaac accactatct tgttgattga acacgatatg aagctggtga tggggatttc
     4261 agaccgaatt tatgtggtca atcaggggac gccactggca aacggtacgc cggagcaaat
     4321 ccgtaataac ccggacgtga tccgtgccta tttaggtgag gcataagatg gaaaaagtca
     4381 tgttgtcctt tgacaaagtc agcgcccact acggcaaaat ccaggcgctg catgaggtga
     4441 gcctgcatat caatcagggc gagattgtca ccctcattgg cgcgaacggg gcggggaaaa
     4501 ccaccttgct cggcacgtta tgcggcgatc cgcgcgctac cagcgggcga attgtgtttg
     4561 atgataaaga cattaccgac tggcagacgg cgaaaatcat gcgcgaagcg gtagcgattg
     4621 tcccggaagg gcgtcgcgtc ttctcgcgga tgacggtgga agagaacctg gcgatgggcg
     4681 gcttttttgc cgaacgcgac cagttccagg agcgcataaa gtgggtgtat gaactgttcc
     4741 cacgtctgca tgagcgccgt gttcagcggg caggcaccat gtccggcggt gagcaacaaa
     4801 tgctggcgat tggtcgcgcg ctgatgagca acccgcgttt gctgctactt gatgagccat
     4861 cgcttggtct tgcgccgatt atcatccagc aaattttcga caccatcgag cagctgcgcg
     4921 agcaggggat gactatcttc ctcgtcgagc agaacgccaa ccaggcgcta aagctggcgg
     4981 atcgcggcta cgtgctggaa aacggccatg tggtgctctc cgatactggt gatgcactac
     5041 tggcgaatga agcggtgaga agtgcgtatt taggcgggta ataacacgtt gattgataag
     5101 gagtcaaaag actcctttga gacaggtgac aaatgtaaaa ttgcctgatg cgctgcgctt
     5161 atcaggccta ctgggtgagt tgcaatatat tgaatttgca cgatcttgta ggccggataa
     5221 ggcgtttacg ccgcatccgg catgaaacga tgagtaatct gtagagtttg attcagacct
     5281 tctatttttc cgcttatccg tgccccatct cccattttcc ctcacccacg ccgtcaccgc
     5341 cttgtcatct ttctgacacc ttacttgcag gtttatgaag ttggcaccgc caccatgatg
     5401 gcatcgaaag ccattaaacc ggtatatgac gtgtttaaag aggcagggat tcaattcgat
     5461 gagtcgcagt ttgtgccgac ggtttccggt tactactccg acagcaaaac gggccactta
     5521 ctctcccagc cattcaacag ctcgactccc gttctctatt acaacaaaga cgccttcaag
     5581 aaagcggggt tagacccgga acagccgccg aaaacctggc aggatctggc ggactcttcc
     5641 gcaaaactga aagcctccgg tatgaagtgc ggctacgcca gcggctggca gggctggatc
     5701 caactggaaa actttagcgc ctggaacggt ctgccgtttg ccagcaaaaa caacggcttt
     5761 gacggcacgg acgcggtgct ggagttcaac aagccggagc aggtgaaaca cattgccatg
     5821 ctcgaagaga tgaacaagaa gggcgacttc agctacgtcg gtcgtaagga tgaatccacc
     5881 gagaagttct ataacggtga ttgcgcgatg accaccgcct cttccggttc tctcgctaat
     5941 attcgtgagt acgccaaatt taactatggc gtaggcatga tgccttacga cgccgatgcg
     6001 aaagatgcac cgcaaaacgc cattatcggc ggggccagcc tgtgggtgat gcagggcaaa
     6061 aataaagaaa cgtataccgg cgtggcgaag ttcctcgact tcctcgcgaa gccagaaaac
     6121 gctgccgagt ggcatcagaa aaccggttat ctgccaatca ccaaagcagc gtatgacctg
     6181 acccgtgagc agggctttta tgagaaaaac ccaggggcgg ataccgcgac gcgtcagatg
     6241 ctgaataagc cgccgttgcc gttcaccaaa gggctgcgtc tgggcaacat gccgcagatc
     6301 cgcgtgattg tggatgaaga gctggagagc gtgtggaccg gtaagaagac accacagcag
     6361 gcactggata ccgccgttga gcgtggaaat cagttgctgc gccgctttga gaaatcgacg
     6421 aagtcttaat cagtgtaatg tcggatgcgt ttcgcttatc tgacctggca tcgcgtgtag
     6481 gccggataag cgaagcgcat ccggcacagt tcaggaatta accgtaatgt catcatcccg
     6541 tccggtgttc cgctcgcgct ggctgcctta tctgctggtc gcgccgcagc tcatcatcac
     6601 cgttatcttt tttatctggc ctgcgggcga agcgttgtgg tactcgcggc aaagcgtcga
     6661 tccgtttggt ttctccagcc agtttgtcgg tctggataac ttcgtcacgc tgttccatga
     6721 cagctactat ctagactcct tctggacgac gataaaattc agcacctttg tcaccgtcag
     6781 cggtttgctg gtatcgctgt tctttgcggc gctggtggag tacatcgtgc gcggcagccg
     6841 tttctatcaa accttgatgc tgctgccgta tgccgtggct cccgccgttg ccgccgtatt
     6901 gtggatcttc ctgtttaacc ccggtcgcgg gctgatcacc cattttctcg ctgagttcgg
     6961 ctacgactgg aaccacgcgc aaaacagcgg tcaggcaatg tttctggtgg tgtttgcctc
     7021 ggtgtggaag caaatcagct acaactttct gttcttctat gccgcgctgc actccattcc
     7081 ccgttcgttg attgaagccg cagctattga cggcgcaggg ccgattcgcc gcttctttaa
     7141 gattgctctg ccgcttatcg ctccggtgag tttcttcctg ctggtggtga acctggtgta
     7201 cgccttcttc gacaccttcc cggtgatcga cgccgccacg tccggcgggc cggttcaggc
     7261 caccactacg ctgatttata agatctaccg cgaaggcttt accggactgg atctggcttc
     7321 gtctgccgcg cagtcggtgg tgttgatgtt cctcgtcatc gtgctgacgg tggtgcagtt
     7381 ccgctatgtt gaaagcaagg tgcgttacca atgattgaga accgtccgtg gctgacgata
     7441 ttcagccata ccatgctgat cctcgggatc gcggtgatcc tcttcccgct gtacgtggcg
     7501 tttgtcgcgg cgacgctgga taaacaggcc gtctatgccg cgccgatgac gctcatcccc
     7561 ggcacacatc tgctggaaaa tatccacaac atctgggtta acggggtagg cacgaatagc
     7621 gcgccgttct ggcggatgtt gcttaacagc tttgtgatgg cgttcagcat tacgctcggc
     7681 aaaattaccg tctcgatgct ctcggcattt gccattgtct ggtttcgttt tccgctacgt
     7741 aacctcttct tctggatgat ttttatcacc ctgatgctgc cggttgaagt acgtatcttc
     7801 ccgacggtgg aagtcatcgc caacctgaaa atgctcgaca gctacgccgg tttaacgctg
     7861 ccgctgatgg cctcggcgac cgccacgttt ctgttccgcc agttctttat gaccctaccg
     7921 gatgaattgg tggaagccgc gcggatcgac ggcgcgtcac caatgcgttt cttttgcgac
     7981 atcgtttttc cgttgtccaa aactaatctg gcggcgctgt ttgtgatcac ctttatctac
     8041 ggctggaatc agtatttgtg gccgttgttg attattaccg acgttgatct cggcaccacc
     8101 gtggcaggga tcaaagggat gatcgctaca ggcgaaggca ccacggaatg gaactcggta
     8161 atggcggcga tgttgttaac gcttatccct ccggtggtga ttgttttagt gatgcagcgt
     8221 gccttcgtgc gcggcctggt cgatagtgag aaataagatg gcaggactga aattacaggc
     8281 agtaaccaaa agctgggatg gcaaaaccca ggtcattaaa ccgctgaccc ttgatgtggc
     8341 ggatggcgaa tttatcgtga tggtcgggcc gtctggctgc gggaaatcga cgctgctgcg
     8401 catggttgcc gggctggagc gggtgacaga aggcgatatc tggatcaacg accagcgcgt
     8461 gaccgaaatg gagccgaaag atcgcgggat tgcgatggtg ttccagaatt acgcgcttta
     8521 tccgcatatg agcgtagagg agaacatggc gtgggggctg aaaattcgcg gcatgggcaa
     8581 gcagcaaatt gccgagcgcg ttaaagaagc ggcgcgcatt ctggaactgg acggtctgct
     8641 taagcgtcgt ccgcgtgagc tatccggcgg tcagcgccag cgtgtggcga tgggccgcgc
     8701 gattgtgcgc gatccggcgg tgttcttgtt tgatgagccg ctctctaacc tcgatgccaa
     8761 gctgcgcgtg cagatgcgtc ttgaactgca acagttgcac cgtcgcctga aaacgacttc
     8821 actctacgtt actcacgatc aggttgaagc gatgacgctc gcccagcgag taatggtgat
     8881 gaacggtggc gttgccgaac agattggcac accagttgaa gtctacgaaa agcccgccag
     8941 cctgtttgtg gcgagtttta tcggcagccc ggcgatgaac ctgctgacag gccgcgtgaa
     9001 taatgaaggc acgcatttcg aactggacgg cggtatcgtg ctaccgctaa acggtggcta
     9061 ccgtcagtat gccgggcgta aaatgactct cggcattcgc ccggaacata tagcgctaag
     9121 ctcgcaggca gaaggtggtg tgccgctggt gatggacacg ctggagatcc tcggcgcaga
     9181 taacctggcg cacggacgct ggggcgagca gaagctggtg gtgcgactgg cgcatcagga
     9241 gcgcccgacg gcaggcagca cgctgtggct gcatttggcg gaaaatcagc tgcatctttt
     9301 tgatggtgaa acaggacaac gagtatgagt aactggcctt atccccgcat cgtcgctcat
     9361 cgtggcggcg gtaagctggc cccggaaaac accctggcgg caatcgacgt cggggcaaaa
     9421 tacggtcata agatgatcga atttgacgcg aagttatcga aagatggcga gatcttcctg
     9481 ctccatgacg acaatctcga acgcaccagc aacggctggg gcgtcgcggg tgaactgaac
     9541 tggcaggatt tactgcgcgt ggatgcgggc agttggtaca gcaaagcgtt taaaggtgag
     9601 ccgctgccgt tgctttcgca ggtggcggaa cgctgtcgcg aacacgggat gatggcgaat
     9661 atcgaaatca aacccaccac tggcactgga ccattaacgg gcaaaatggt ggcgctggcg
     9721 gcacgccaac tgtgggccgg tatgacgccg ccgctgctgt catcgtttga gattgatgct
     9781 ttagaagctg cacaacaggc ggcaccggaa ctgccgcgtg gtttattgct tgatgagtgg
     9841 cgcgacgact ggcgcgaact gaccgcgcgg ctgggctgcg tctctattca tctcaatcat
     9901 aagttactcg ataaagcgcg agtgatgcag ttgaaagacg ccggattacg gattctggtt
     9961 tataccgtca ataaacccca gcgcgcggca gagttgctgc gctggggcgt ggattgcatc
    10021 tgtaccgatg cgattgacgt gattggtccg aactttacgg cccaatagtt ttcaacggaa
    10081 tgtcaggctg cggcgtgctg ggctgattca gcatgtcgcc gtttctcctt tccggcagca
    10141 tatgctgctg attcaacgaa ctatccggat tacggttgct gtttaacatc ccgccgttgg
    10201 tgttgggcag catttgctgc cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc
    10261 gctgagaatt attgtttatc tggttttcta aatgctgctg ttgcaactga gtttgcgttt
    10321 tcagttgctg attcagcatc cctttttgct ggatttgctg agtctgcatc tgggtttgca
    10381 tccgctgctg gctgggtatc tgataccccg gctggttagg gttgttcaga gtattaatgg
    10441 gctgtgcaaa gccgacaaac ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt
    10501 atcctcctct gaagatatcc tttaagttta ctcgcttccc gacaaaacga tgattaattc
    10561 agagttatat accaggctta gctggggttg ccccttaatc tctggagaat aacgatgata
    10621 aaaccgacgt ttttacgccg ggtggccatt gctgctctgc tctcaggaag ttgttttagc
    10681 gccgccgccg cgcctcctgc gccgcccgtc tcgtatggtg tggaggaaga tgtcttccac
    10741 ccggtacgcg cgaaacaggg aatggtagcg tctgtggacg ccactgccac tcaggtgggg
    10801 gtggatatcc tcaaggaggg cgggaatgcc gttgatgccg ccgtggcggt gggctacgcg
    10861 ctggcggtaa cgcatccgca ggcagggaat ctgggcggtg gtggttttat gttaatccgc
    10921 tcgaaaaatg gcaataccac ggctatcgat ttccgcgaaa tggcacccgc caaagcgacc
    10981 cgcgatatgt tcctcgatga tcagggcaac ccggacagca aaaaatcact cacttcgcat
    11041 ctggcttccg gcacaccggg tacggtagca ggtttctcgc tggcgctgga taaatacggc
    11101 accatgccgc tgaacaaagt cgtgcagccc gcgtttaaac tggcacgcga tggttttatc
    11161 gttaacgacg cgctggctga cgatctcaaa acctacggta gcgaagtgtt gccgaatcac
    11221 gaaaacagta aagctatctt ctggaaagag ggcgagccgc tgaaaaaggg cgacacgctg
    11281 gtgcaggcga acctggcaaa gagcctggag atgattgctg aaaacggccc ggacgaattc
    11341 tataaaggca cgattgcgga acagatcgcc caggagatgc agaaaaacgg tggcttgatc
    11401 actaaagaag atttagcggc ctataaagcg gtcgaacgca ctccgataag cggcgattat
    11461 cgcgggtatc aggtttactc catgccaccg ccatcctccg gcgggatcca tatcgtacaa
    11521 atcctcaata ttctggaaaa cttcgatatg aagaaatacg gctttggcag cgccgatgcg
    11581 atgcaaatca tggcagaagc ggagaaatac gcctacgccg accgctcgga atatcttggc
    11641 gacccggatt ttgtcaaagt cccgtggcag gcactgacca ataaagccta tgccaaatcc
    11701 attgccgatc aaattgatat caacaaagcg aagccgtcca gtgagattcg ccctggcaag
    11761 cttgcgcctt atgagagtaa tcaaactacc cattactctg tagtggataa agacggtaac
    11821 gcggtggcgg tgacctatac gctgaacacc accttcggta cgggcattgt cgcgggcgag
    11881 agcggtattc tgcttaataa tcagatggat gatttctccg ccaaaccggg cgtaccgaac
    11941 atttacgggc tggtgggcgg tgatgccaac gccgtcgggc cgaataaacg tccgctgtca
    12001 tcgatgtcac caaccattgt ggtgaaagac ggtaaaactt ggctggttac cggtagtcct
    12061 ggcgggagtc ggattattac caccgtgc
//
LOCUS       DAZOXR010000126        12038 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZOXR010000126 DAZOXR010000000
VERSION     DAZOXR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.128"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHUNN_003015"
     CDS             <1..323
                     /locus_tag="ACHUNN_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577325.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHUNN_003016"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHUNN_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHUNN_003017"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHUNN_003017"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0577326.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="ACHUNN_003018"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="ACHUNN_003018"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0577327.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHUNN_003019"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHUNN_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0577328.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHUNN_003020"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHUNN_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0577329.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHUNN_003021"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHUNN_003021"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0577330.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="ACHUNN_003022"
     CDS             8424..8927
                     /locus_tag="ACHUNN_003022"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0577331.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="ACHUNN_003023"
     CDS             9004..9687
                     /locus_tag="ACHUNN_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0577332.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="ACHUNN_003024"
     CDS             9766..10752
                     /locus_tag="ACHUNN_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0577333.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="ACHUNN_003025"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="ACHUNN_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0577334.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2857 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcatt
//
LOCUS       DAZOXR010000127        11959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZOXR010000127 DAZOXR010000000
VERSION     DAZOXR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.129"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1759
                     /locus_tag="ACHUNN_003026"
     CDS             <1..1759
                     /locus_tag="ACHUNN_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0577335.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2095..3072
                     /gene="glaH"
                     /locus_tag="ACHUNN_003027"
     CDS             2095..3072
                     /gene="glaH"
                     /locus_tag="ACHUNN_003027"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0577336.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3092..4360
                     /gene="lhgO"
                     /locus_tag="ACHUNN_003028"
     CDS             3092..4360
                     /gene="lhgO"
                     /locus_tag="ACHUNN_003028"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0577337.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4383..5831
                     /gene="gabD"
                     /locus_tag="ACHUNN_003029"
     CDS             4383..5831
                     /gene="gabD"
                     /locus_tag="ACHUNN_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0577338.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5845..7125
                     /gene="gabT"
                     /locus_tag="ACHUNN_003030"
     CDS             5845..7125
                     /gene="gabT"
                     /locus_tag="ACHUNN_003030"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR0577339.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7436..8836
                     /gene="gabP"
                     /locus_tag="ACHUNN_003031"
     CDS             7436..8836
                     /gene="gabP"
                     /locus_tag="ACHUNN_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0577340.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8857..9519
                     /gene="csiR"
                     /locus_tag="ACHUNN_003032"
     CDS             8857..9519
                     /gene="csiR"
                     /locus_tag="ACHUNN_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0577341.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9520..9969)
                     /gene="kbp"
                     /locus_tag="ACHUNN_003033"
     CDS             complement(9520..9969)
                     /gene="kbp"
                     /locus_tag="ACHUNN_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0577342.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10053..10211)
                     /locus_tag="ACHUNN_003034"
     CDS             complement(10053..10211)
                     /locus_tag="ACHUNN_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0577343.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10394..10693
                     /locus_tag="ACHUNN_003035"
     CDS             10394..10693
                     /locus_tag="ACHUNN_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0577344.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10703..11227
                     /gene="ygaP"
                     /locus_tag="ACHUNN_003036"
     CDS             10703..11227
                     /gene="ygaP"
                     /locus_tag="ACHUNN_003036"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0577345.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11274..11678)
                     /gene="stpA"
                     /locus_tag="ACHUNN_003037"
     CDS             complement(11274..11678)
                     /gene="stpA"
                     /locus_tag="ACHUNN_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0577346.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2984 a         2947 c         3078 g         2950 t
ORIGIN      
        1 tgtatataat attaacgtta cgtcaccttc cggacagcaa gaaacattta aaatactaat
       61 caatccagaa catctacggc agacacttga gcgtaaatct cttactgctg ttcagaaatc
      121 acaatgtgaa atcatcaccc ctaaaaaacc cggcgaagcg attcttcatg cttttaatgc
      181 cacctaccag caaatcaggg aaaatatgtc tgagtttgca cgttgccatt atgggtatat
      241 acaaatccct ccagtgacaa ctttccgcgc cgacggacca gaaactcccg aagaagaaaa
      301 gggttactgg tttcatgctt atcaacccga agatctttgt accatccata atccaatggg
      361 agatttgcag gattttattg cattggttaa agatgctaaa aaatttggta tcgatatcat
      421 tcctgattat acctttaact ttatgggaat tgggggtagt ggtaaaaatg acctggatta
      481 tccctctgct gatatacgag cgaagatcag taaagatata gaaggtggta tccctggcta
      541 ttggcaaggt caggttttga ttccattcat taaagatcca gtaacaaaag aacgtaaaca
      601 aatccatcca gaagatatac atctcactgc aaaagacttc gaagcaagta aagataacat
      661 ctctaaggat gaatgggaaa acctccatgc attaaaagaa aagcgtttaa atggaattcc
      721 taaaacaaca cccaaaagtg accaggttat tatgttgcaa aatcaatacg ttcgtgaaat
      781 gcgaaaatat ggcgtacgag gtttacgtta tgatgcggca aaacactcaa aacatgaaca
      841 aatagaaaga tcaataaccc caccgcttaa aaattataat gagcggttac acaatactaa
      901 cttatttaac ccaaaatatc ataaaaaagc cgttatgaat tacatggaat atctggtaac
      961 ttgtcagttg gatgaacaac aaatgtcatc actgctttat gaaagagatg atttaagcgc
     1021 cattgatttt tcattgctca tgaagacgat aaaagccttt tcattcgggg gagatctcct
     1081 aacccttgca tcaaaaccgg gttccacaat ctcaagcatc ccatcagaaa gacggatatt
     1141 gattaacatt aaccacgatt ttcctaacaa tggcaatctt ttcaatgact ttctatttaa
     1201 ccatcaacaa gatgaacaat tagcaatggc atatatggcc gctctcccgt tcagcagacc
     1261 tttagtttac tgggatggcc aggtattaaa atcaacgact gaaattaaaa attatgatgg
     1321 gtccacgcgt gtcggtggtg aggcgtggct taataaaggt tgctctacct atcagcagct
     1381 ctacaatgaa ttccacgcat tatatataga taaagcagga atatggagcg catttgaggg
     1441 tgtatttgca actaaaaacg ttctagcctt tagtcgtggg gattctgtga acattaatca
     1501 ctctcctcat gatggactag ttataataaa taaaggaaac gaagaagttg aaggtacctg
     1561 gcctaacaaa ttgcaacctg gaatatataa aaacatgggg agtaatagcg ttaacattat
     1621 tattaataat acccgaaaaa ttatcccccc tggtaaagta tttacgctta gaggcggaac
     1681 tctaaatatc aatattcctg ggcgtagcgc tcttctttta gggaaaacag gagaaccgcc
     1741 gaactatctc tatttataat ttaatgttat atctgccccg ataaaacggg gcagataata
     1801 tgtttagttt actaacggtc attttgcagt gaagccattt actgtttttt atcgaccaga
     1861 taatctgttc tctaatgtta actcccccta acctgttgct ttagttattc atttcctgtc
     1921 tcactttgcc ttaatactct acgttaaatg ttactaattt gttgcttttg atcacaataa
     1981 gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg tagatatttt tagattatgg
     2041 ctacgaaatg agcatcgcca tgtcacccta catctcataa gaggatcgct tctgatgaat
     2101 gcactgaccg ccgtacaaaa taacgctgtc gattcaggcc aggactatag cggattcact
     2161 ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca ccttcaccga acagacgacc
     2221 aaacagtttc tcgagcaggt tgccgaatgg cccgtacagg cgctggagta caaatcgttt
     2281 ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg cgaatcagct gcaaccattg
     2341 ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt tgatcaatgc ggtgggtatc
     2401 gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa cggcggtagc gcatctgatt
     2461 ggtcgctcaa atttcgatgc gatgagcggt cagtattacg cgcgtttcgt ggtgaaaaat
     2521 gtcgataact cagacagcta tctgcgtcag ccgcaccgcg tgatggaact gcacaacgac
     2581 ggcacgtatg tcgaagagat caccgattac gtgctgatga tgaaaatcga tgagcaaaac
     2641 atgcagggtg gaaattcgtt gctgctgcat ctcgatgact gggaacatct ggaccactat
     2701 ttccgccatc cactggcgcg tcgcccgatg cgctttgccg cgccgccgag caaaaacgtc
     2761 agcaaagatg ttttccatcc agtgttcgac gttgatcaac agggtcgccc ggtgatgcgc
     2821 tatatcgacc agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt gagcgagctt
     2881 tccgatgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt tggcaaattc
     2941 ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc gcacccggat
     3001 ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca ccactaccag
     3061 acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta ttggcggcgg
     3121 catcatcggc atgtcgaccg ccatgcaact gattgatgtt tatccggatg cccgcattgc
     3181 gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca gcggcgtgat
     3241 ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc tggcggggaa
     3301 ccgcgccacc aaagcctttt gcgatcaaaa cggcattcgc tatgacaact gcggcaagat
     3361 gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc gccttatggg aacgcacagc
     3421 ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa ctgcgcgagc gcgaaccgaa
     3481 tatcaccggg cttggtggca tttttgtgcc gtccagcggc attgtcagct accgcgaagt
     3541 cacagcggca atggcaaaaa tcttccaggc cagaggcggc gagattattt ataacgccga
     3601 agtcagcgcc ctcagtaagc ataaaaacgg cgtggtgata cgcacccgtc agggcggtga
     3661 atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc tggtgaaaat
     3721 gctcggcctc gaaccgggct ttatcatctg cccgttccgt ggtgagtatt tccgccttgc
     3781 gccggagcat aaccagattg ttaaccacct gatttacccc attcccgacc ccgcgatgcc
     3841 atttttgggc gtgcatctca cccgcatgat cgacggcagc gtgaccgtcg ggccaaacgc
     3901 ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta gtgacacgct
     3961 ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa aaccatctac gctcaggact
     4021 gggcgagatg aaaaactcgc tgtgcaaaag cggctacctg cggctggtgc aaaagtattg
     4081 tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg cgcaggcagt
     4141 atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac gcacgatcca
     4201 cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg cgcatattgt
     4261 cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc tgcgagcggc
     4321 acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa caggacgtaa
     4381 caatgaaact taacgacagt aacttattcc gccagcaggc gttgattaac ggggaatggc
     4441 tggacgccaa caatggcggg atcatcgacg tcaccaatcc ggcgaacggc gacaagctgg
     4501 gtagcgtgcc gaaaatgggc gctgatgaaa cccgcgccgc tatcgacgcc gccaaccgcg
     4561 ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caacattctg cgcaactggt
     4621 tcaatttgat gatggagcat caggacgatt tagcgcgcct gatgaccctc gaacagggca
     4681 aaccactggc cgaagcgaaa ggcgaaatca gctacgctgc ctcttttatt gagtggtttg
     4741 ccgaagaagg caaacgtatt tatggcgata ccattcccgg tcatcaggcc gataagcgcc
     4801 tgattgttat caagcagccg attggcgtta ccgcagctat cacgccgtgg aacttcccgg
     4861 cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggctgcacc atggtgctga
     4921 agcccgccag tcagacaccg ttctctgcgc tggcgctggc ggagctggcg attcgcgcgg
     4981 gcattccggc tggcgtgttt aacgtagtca ccggttcggc aggcgcggtc ggtaatgaac
     5041 tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ttcgaccgaa attggccgcc
     5101 agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg ggcggcaacg
     5161 cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc gcgctggcct
     5221 cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac gtgcaggacg
     5281 gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt gagcaaactg cacatcggcg
     5341 acgggctgga taaaggcgtc accatcgggc cgctgatcga tgaaaaagcg gtagcaaaag
     5401 tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc ggcggtaaag
     5461 cggatgaacg cggcggcaac ttcttccagc cgaccattct ggtggacgtg ccggccaacg
     5521 ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc gctgttccgc tttaaagatg
     5581 aagccgatgt gattgcgcaa gccaatgaca ccgagtttgg ccttgccgcc tatttctacg
     5641 cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct ggagtacggc atcgtcggca
     5701 tcaataccgg cattatttcc aatgaagtgg ccccgttcgg cggcatcaaa gcctcgggtc
     5761 tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa tatatgtgca
     5821 tcggtcttta actggagaat gcgaatgaac agcaataaag agttaatgca gcgccgcagt
     5881 caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg cgcggaaaac
     5941 tgccgggtgt gggacgttga aggccgtgag tatcttgatt tcgcgggcgg gattgcggtg
     6001 ctcaataccg ggcacctgca tccgaaggtg gtggccgcag tggaagcgca gttgaaaaaa
     6061 ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct gtgcgagatt
     6121 atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac gaccggttcc
     6181 gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag cggcaccatc
     6241 gcttttagcg gcgcgtatca cgggcgcacg cattacacgc tggcgctgac cggcaaggtg
     6301 aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc gctttatcct
     6361 tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccaccg gatcttcaaa
     6421 aatgatgccg cgccggaaga tatcgccgcc atcgtgattg agccggttca gggcgaaggc
     6481 ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac gcgctctgtg tgacgagcac
     6541 gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg cacgctgttt
     6601 gcgatggagc agatgggcgt ggcgccggat cttaccacct ttgcgaaatc gatcgcaggc
     6661 ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga tggacgctgt tgcaccgggc
     6721 ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggctgcgct ggaagtgttg
     6781 aaggtgttcg agcatgaaaa tctgctgcaa aaagccaacg atctggggca gaagctaaaa
     6841 gacggattgc tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg cgggctggga
     6901 gcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga cgcaaaactc
     6961 accgccgaga tcgtggcacg cgcccgcgat aagggcctga ttcttctctc ctgcggcccg
     7021 tattacaacg tgctgcgcat ccttgtaccg ctcaccattg aagacgctca gatccgtcag
     7081 ggtctggaga tcatcagcca gtgttttgct gaggcgaagc agtagctatg tccgtttgta
     7141 agagttaacc gttgctaagg gaatgataac gatgcagtaa aaaataataa cgatgaggcg
     7201 aacgacaaag ttaaaaacga ctgcggacag tcccctcgcc cctctggaga gagggttagg
     7261 gtgaggggta aaacacgcgg ctaaaccctc ccctggccct ctccctaagg gagagggaga
     7321 aaaccctccc gctttcgttt ttaagatttt gttgaccgtc tcaattaaac gcgtgacccg
     7381 gcgcgggaaa tgtcggggcg ctctcccaag tgacacactt tcgagaggat tcaggatggg
     7441 gcaatcatcg caaccacatg agttaggcgg cgggctgaaa tcacgccacg tcaccatgtt
     7501 gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga
     7561 agcgggcccg gcggtattac tggcctatct gttcgccggg ctactggtgg ttatgattat
     7621 gcggatgttg gcggaaatgg cggttgccac gcccgatacc ggttcgtttt ccacctatgc
     7681 cgataaagcc attggtcgct gggcgggcta taccatcggc tggttgtact ggtggttttg
     7741 ggtactggtt atcccgctgg aagccaatat cgccgctatg atcctgcact cgtgggttcc
     7801 aggcattccc atctggttat tttccctcgt cattaccctt gccttaactg gcagtaattt
     7861 attaagcgtt aaaaactacg gcgaatttga gttctggctg gcgctgtgca aagtcatcgc
     7921 tatcctggcc tttattttcc ttggtgcagt cgcaattagc ggtttttacc cttatgccga
     7981 agtgagcggg atctcaagat tgtgggatag cggcggcttt atgcccaacg gtttcggtgc
     8041 ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcaccat
     8101 tgccgccgcg gaatccgaca cgccggaaaa acatattgtc cgcgccacca actcggttat
     8161 ctggcgtatt tctatcttct atttgtgctc tatttttgtc gtcgtggcat taataccgtg
     8221 gaatatgccg gggctgaaag ccgttggatc ttatcgctcg gttctggaat tgctcaatat
     8281 tccccatgcg aaattaatca tggactgtgt gatattactt tccgtaacca gttgcctgaa
     8341 ctcggccctg tataccgcct caaggatgct ctactcctta agccgtcgcg gtgatgctcc
     8401 cgcggtaatg ggtaaaatca accgcagtaa aaccccgtac gtggcggtgt tactctccac
     8461 cggcgcggca ttcttaacgg tggtggtgaa ctattacgcg cctgcgaagg tatttaaatt
     8521 tctgatcgac agctccggcg ctatcgccct gctggtttat ttagtcatcg ccgtttcaca
     8581 gttgcggatg cgcaaaattc tgcgagcgga aggaagcgaa attcgcttgc gaatgtggct
     8641 ttatccctgg ctcacctggc tggtcatcgg ctttattacc tttgtgttgg tagtgatgct
     8701 attccgtccg gcgcaacagt tagaagtgat ctccaccggc ttattagcga tagggattat
     8761 ctgtaccgtg ccaattatgg ctcgctggaa aaagctggta ttgtggcaaa aaacacccgt
     8821 tcataatacg cgctgataat gatcaggagt cacaccatga ccattacgtc tctggatggc
     8881 tatcgctggc tgaagaacga tattattcgc ggtaattttc aaccggatga aaaattacgg
     8941 atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc cgttgcggga agcactttct
     9001 caactggtgg cggaacggct ggtcacggtg gtgaatcaaa aagggtatcg ggtggcgtct
     9061 atgtcagagc aggagctgct cgatattttc gatgcccgcg ccaatatgga agcgatgtta
     9121 gtgagtctgg cgattgcccg cggtggcgat gagtgggagg cagacgttct cgcaaaagcg
     9181 catctgctca gtaagctgga agcctgtgac gccagcgaga aaatgcttga tgagtgggat
     9241 ctgcgtcatc aggcgtttca tacagcaatt gtggcgggct gtggttctca ctatttgctg
     9301 caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc gatttatctg gctgcggcga
     9361 acggtgcttt cggtagaaat gctggaggat aaacacgatc agcaccagac cctgactgcg
     9421 gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa tgcgccagca tttactgacg
     9481 ccaattccca ttatccagca ggcgatggct ggcaattaac tactcttccg gaatacgcaa
     9541 cacttgcccc ggataaattt tatccgggct ttttagcatc ggtttattcg cttcgaagat
     9601 tttattgtac agattagcgt taccgtagac ctgtttggaa atggcactca gagtgtcgcc
     9661 agacttaacg gtataaaact ggctggcagt ggctggtgtc gccgttttca cctgatcatc
     9721 aacactggca ataccggaaa tattccccac cgcaacaagg attttctcct tcgcctcctg
     9781 actcaggccg tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt tcactttatc
     9841 agcatcaggt ataccggttt tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt
     9901 atcgtgctga cctgtaaccg cgtcccagag tttttctccg gcatctttca caaaattgaa
     9961 cagacccata gctacctcat tcgttaacgg aaacactcag aaagtgtagc agaggaacgc
    10021 ggcgcgttaa cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg
    10081 tgaatcaaac cggggatata cccaagcagt gtcagcagaa tattaataat gaacgcccaa
    10141 ccgaaccctt taccgagcag cacgccgagc ggcggtagaa tgatggtgat gacgattctc
    10201 cagaaaccca tatgtactcc ctataagaaa attactcatt gtttaaaaag agattttatc
    10261 tcttaagcgt agtaatttta gcggagcctg ccagtttcag cttacgcttt tactctcatt
    10321 tacgctgctg aggctggtat atgttgcatt tttagtttag agcttactta atttagaaaa
    10381 tacttaaata aatatgactg aactcgcgca attacaggcc agtgccgaac aggcagcggc
    10441 cttattgaaa gcaatgagcc atcctaaacg attgctgatt ctgtgcatgc ttagcggttc
    10501 ccccggcacc agcgcgggag agctgacgcg cattaccgga ctgagtgcct ctgcgacatc
    10561 acagcatctc gctcgtatgc gggacgaagg gcttatcgac agccaacggg atgcccaacg
    10621 cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt
    10681 ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga
    10741 attaatcgca cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga
    10801 acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc
    10861 taaattacgt cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa
    10921 cgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat
    10981 tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt
    11041 aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta
    11101 taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc
    11161 aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt ggaaccaacg
    11221 agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc
    11281 aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg
    11341 ccctgaccgg tccaggtttt agtttcaccg ttaacatcgg tgaatttata tttcgccgga
    11401 cgcggctggc gttttttacc agcgcgtggt gcagcagcag agctattacc caataactct
    11461 tccgggttaa ttccgtcagc cttcatcagc tccagccagg tgctaatttt ttcctgacgc
    11521 tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg
    11581 aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg
    11641 gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc
    11701 caaaacaaaa aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa
    11761 tttaatctat ttacagtctt gcgcgagatt atttcagctg ttattttctg aaactctcag
    11821 aataatttca agattatata ttagccattt tgatgagtaa tttagattat tggtgaacag
    11881 aacggcgctc tctgctcgct aattccgcaa gcctgacaaa accgaagtga ataaaaatgc
    11941 aatataacag aataaaaga
//
LOCUS       DAZOXR010000128        11911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZOXR010000128 DAZOXR010000000
VERSION     DAZOXR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.130"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            29..595
                     /locus_tag="ACHUNN_003038"
     CDS             29..595
                     /locus_tag="ACHUNN_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0577347.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            751..2783
                     /locus_tag="ACHUNN_003039"
                     /pseudo
     CDS             751..2783
                     /locus_tag="ACHUNN_003039"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2760..3104
                     /locus_tag="ACHUNN_003040"
     CDS             2760..3104
                     /locus_tag="ACHUNN_003040"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR0577348.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3119..3850
                     /locus_tag="ACHUNN_003041"
     CDS             3119..3850
                     /locus_tag="ACHUNN_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0577349.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3847..4908
                     /locus_tag="ACHUNN_003042"
     CDS             3847..4908
                     /locus_tag="ACHUNN_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0577350.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5060..6106
                     /locus_tag="ACHUNN_003043"
     CDS             5060..6106
                     /locus_tag="ACHUNN_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0577351.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6103..6894)
                     /gene="nei"
                     /locus_tag="ACHUNN_003044"
     CDS             complement(6103..6894)
                     /gene="nei"
                     /locus_tag="ACHUNN_003044"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0577352.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6930..7664)
                     /gene="pxpA"
                     /locus_tag="ACHUNN_003045"
     CDS             complement(6930..7664)
                     /gene="pxpA"
                     /locus_tag="ACHUNN_003045"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0577353.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7654..8586)
                     /gene="pxpC"
                     /locus_tag="ACHUNN_003046"
     CDS             complement(7654..8586)
                     /gene="pxpC"
                     /locus_tag="ACHUNN_003046"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR0577354.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8580..9236)
                     /gene="pxpB"
                     /locus_tag="ACHUNN_003047"
     CDS             complement(8580..9236)
                     /gene="pxpB"
                     /locus_tag="ACHUNN_003047"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0577355.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9259..10002)
                     /gene="ybgI"
                     /locus_tag="ACHUNN_003048"
     CDS             complement(9259..10002)
                     /gene="ybgI"
                     /locus_tag="ACHUNN_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0577356.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10273..11754
                     /gene="dtpD"
                     /locus_tag="ACHUNN_003049"
     CDS             10273..11754
                     /gene="dtpD"
                     /locus_tag="ACHUNN_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0577357.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2835 a         3178 c         2929 g         2969 t
ORIGIN      
        1 tttttctttt ctgttaaagg actcagttat gtttaaagga caaaaaacat tggccgcact
       61 ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat ccggtgaaat
      121 tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg atattgataa
      181 agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc acagcgatgc
      241 cgttgctgtc gacctgcgct tagtcaactg tgatctggaa aactccagca acggttccgg
      301 tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca ccggcgcaga
      361 tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac gtttaatgaa
      421 taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc tggctccgac
      481 ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc aggctacacc
      541 tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata aataagtatt
      601 actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga caccgtgaat
      661 atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc attggcggtc
      721 gaattcaacc ttaatgttct cgataaatcg atgcgcgacc gcattgatat ttcattatta
      781 aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt aaataataac
      841 caaatcagta acgggcaaaa gattaactgg cacaaaaatg acgataaaac cattccgtgc
      901 atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca gtcgttacca
      961 ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa
     1021 gccaatcagc aactgaatat tagtattccg caagcctggc tggcgtggca ctcagaaaac
     1081 tggaccccac cctccacatg gaaagaaggt gtcgccggta tcctgatgga ttacaacttg
     1141 tttgccagca gctaccgccc acaggacggc agcagcagca ctaacctgaa cgcctacggt
     1201 accaccggaa ttaacgccgg ggcatggcgc ttacgtagtg attatcagtt gaatcagact
     1261 gatagcgatg ataaccatga acagtcaggc ggaatatcgc gcacctatct ttttcgtcca
     1321 ttaccgcaat taggctctaa attaaccctc ggcgaaacgg attttagttc caatattttc
     1381 gacggttttt cttataccgg cgcggcactg gcaagtgacg agcgaatgtt gccatgggaa
     1441 ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac ggtgacgatc
     1501 agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt tatcattgac
     1561 gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga agatggtcgg
     1621 gtgaacaatt tccaggtttc ggcagcatcg acgcccttcc tgactcgtca gggacaggtt
     1681 cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa
     1741 acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct gtacggcggc
     1801 ctgctgcttt ctggtgatga ctaccattct gccgcaatgg gtattgggca aaatatgctg
     1861 tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta gccattttga tactcagcag
     1921 gacgagcggg gcttaagcta ccgttttaat tacagcaaac aagtggatgc cactaacagc
     1981 acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta cgccaactat
     2041 ctggatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat cagcttattg
     2101 tgggccaacc gattacccca ctaaacctca atctttacgc caacctgcta catcaaacct
     2161 ggtggaatgc agacgcctcc acgaccgcca acatcacggc aggttttaat gttgatattg
     2221 gtgactggag agatatctct atttcgacgt cattcaatac aacccattac gaagataaag
     2281 atcacgacaa ccagatttac ctgtcgattt cgctcccctt cggtaacggt ggtcgggttg
     2341 gctatgacat gcaaaacagt agccacagca ccacacaccg catgtcgtgg aacgatacgc
     2401 tggatgaacg taatagctgg ggcatgtctg ccggactgca atccgaccgt cctgacaatg
     2461 gagcccaggt gagcggtaac tatcagcacc tgagttcagc gggtgagtgg gatatttctg
     2521 gtacctatgc cgccaatgat tacagttccg tcagcagcag ctggagcggt tctttcaccg
     2581 caacccaata tggtgcagcg tttcatcgcc gcagctccac caatgaacca cgcctgatgg
     2641 tcagcaccga tggcgtggca gatattccgg ttcagggcaa tctcgactac accaaccatt
     2701 ttggcattgc ggtggtgccg ttgatttcca gttaccagcc ttccaccgtg gcggtgaaca
     2761 tgaatgactt acccgacggc gtaacagttg cagaaaacgt tatcaaagaa acgtggattg
     2821 aaggcgcgat aggttacaaa tcactggctt cccgttccgg taaagacgtt aacgtcatca
     2881 ttcgcaacgc cagcggtcag tttcctcccc tcggagcgga tatccgccag gatgacagcg
     2941 gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa
     3001 atcaaaagtt taccgtggtc tggggtgata gccagcattg ctcgctccat cttcctgaac
     3061 atatggaaga caccgcaaat cgcctgattt taccttgtca ttaattaagg aaatgaaaat
     3121 gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca gccacgctgc
     3181 actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta ccagtctgcg
     3241 ggtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg aaaatgaaaa
     3301 aggggagaaa agcgatgatc ttctggtcgc cctgccgccg atacaacgtc tggaaccgaa
     3361 agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac cgggcgatcg
     3421 cgaaacgcta tttttctaca atatgcgtga aattcctccc gccccggaaa aaaacagcga
     3481 ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtgttctggc gaccggctgc
     3541 attacgcaag aaagcaggag aaaaggtcga actgcagtta caggtcagcc agcagggcaa
     3601 ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctacc tgggacgaaa
     3661 tgaaaaaggc gttctccccg gcttcaaaac cgtgatggtt gcgcctttta gtacggtcaa
     3721 cacaaacacc ggaaattata gcggcagcca gttttatctc ggttatatgg atgattacgg
     3781 cgcgttacgc atgacgacgc tcaactgcag cggacaatgc catttacaag ccgtggaggc
     3841 gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctgttatt tctgccgttg
     3901 aagtctgcgc ttgccctgaa ctgctacttt ggttcatccg gtggctcggt agaaaaatca
     3961 gaagcgattc aaccttttgc tgtaccaggc aatgccaaac tcggcgataa gatttgggaa
     4021 tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa ttttgaaagt
     4081 gagcatgttt atgcctgggt aaacccctac ccgggggtgc aggatcgcta ttatcaactc
     4141 ggcgtaactt acaatggcgt tgattatgac gccaaccagg gaaaaagccg catcgacact
     4201 aaccagtgta ttgacagtaa gaacattgac atttataccc ctgagcagat tatcgcgatg
     4261 ggatggcaga acaaaatttg ctcgggtgat cccgctaata ttcacatgtc gcgtaccttt
     4321 ctcgctcgca tgcggttata cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt
     4381 acgcttagcg actatatcgt cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct
     4441 actgcccaaa acctgaaata tcatattact ggtctggaaa acattcgcgt gctggattgc
     4501 agcgtcaatt tttccatttc accggaaaca caggtgattg attttggtaa atttaatttg
     4561 ctggatattc gtcgccacac aatgtcgaaa acattcagta ttaagacgac aaaatcacaa
     4621 aatgaccaat gcaccgacgg atttaaagtc agttcctcct tttataccga agaaacgttg
     4681 gttgaagaag ataaagcatt gttgattggt aatggtctga agctacgttt gctggatgaa
     4741 aacgcctcgc cttatacctt caataaatat gctgaatacg ccgatttcac cagcgacatg
     4801 ttggtctacg aaaaaaccta tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag
     4861 gctgggccct tcgatacagt ggtgcttttt aaaattaact ataactgatt tgccatgatg
     4921 ccctttgcag ccggaaaggg catctctttc agaaaattgc tgattttccc cttttccttg
     4981 attgctgtta ttttttcctg gcccggcttt ctttatgctg tatcgataca ccaaagagaa
     5041 aataatgagg gagcgaagga tgccagtttt gcagtgggga atgttatgtg tgctatcact
     5101 tctcctttct attggtttcc tcgcggtaca tctcccggcg gcgctattac ttgggccgat
     5161 gatcgccggg atcatcttca gtatgcgcgg aattactctg caacttcccc gctccgcttt
     5221 tctcgccgcg caggccattc ttggctgcat gattgcgcaa aacctcactg gttcgattct
     5281 caccacgctg gccgccaact ggccgatcgt gcttgctatt ttactggtga cgctgctttc
     5341 cagcgccatc gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggtgc
     5401 ctggggttct tctccaggcg gcgcagcggc aatggtcgct atggcgcaag attacggcgc
     5461 agatattcgc ctggttgcat ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc
     5521 tgttctggtc acccgcatga tgctgggtga taacgctgaa gcggttaacc agcaaatcgt
     5581 ctggttcccg ccagtgagca ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg
     5641 tacggcggga tgcatgctgc gtcttccttc cggcacgatg ctcatcccga tgctggcggg
     5701 cgcagtgctc cagtctggtc agctcatcac catcgaacta ccggaatggc tgctggcgat
     5761 ggcgtatatg gcaattggct ggcggattgg tcttggtttc gataaacaaa tcttactgcg
     5821 ggcactacgc ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc
     5881 gggtatggcg tgggggctga cccggtttat gcacattgat tttatgactg cctacctcgc
     5941 caccagtccc ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat
     6001 ggcgcttatt atggcgatgc aaaccctgcg cctgttcagt attttgctga cggggccagc
     6061 cattgcgcgg tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct ggcagccagg
     6121 gcaccagtaa aacgggcgag atgacagcgt ggttttctca atgatgctgc cacagcgttc
     6181 gcacggttcg ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatgcttatt
     6241 ctcatccacc agccccctcg tagcgtagga aaatcgagga atatccagta acgcgtgtgc
     6301 gagtgcatcc agttgcgccg cattgagatc tttcgcttta tgatttccag tcaacccaac
     6361 ctgccagagg atctccaccc gcaaataatt gccaagccca gccagaaacg cctgatcgag
     6421 cagtaatcct gcaaactgac ggttacgaaa gcgcggtgac aataaccgtt cctttaccac
     6481 ctccggcgtc agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt
     6541 ggtcagttgt tccggcctca acatctcaat atccgaggcg ctataaagca gaatggtttt
     6601 gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc
     6661 aaccacgcgc cagacgccgt aaagctgatt atggctgtag agcgttaagt cgttggaaaa
     6721 atgagttaac aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagttg
     6781 tgattgataa gtttttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt
     6841 gatcgccgcc tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat
     6901 atcctttttt cattctttat taatcggggt tatgctgcga caacaatctc cttttcggca
     6961 aatgtagagc gtagtcggcg ggcgaacgcc agtgcgtgct cgccgtcgcc atgcaggcag
     7021 actgtttgcg ccgtgaccgt tgcccattcg ccggtgatac ttttgactct gccgtgttgc
     7081 accatctcta gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc gcctcgctga
     7141 cttcgcggca ccagcgagcc gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc
     7201 gttgtcaggc catattgctt gcctgcacga atcagctcgc ttcctgccag cccaacgaga
     7261 atcaatgccg gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcttct
     7321 ttcgccgcct ggttgtacaa catgccgtgc ggtttgacat gacgcattac gccgccttgc
     7381 gcacgggcaa tcgttgccag cgcgccaatt tgatacagcg tctgggcgta aacggtttct
     7441 ggcggcagct gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg
     7501 ccaatcgcga caccattttt tattgcttca cgcacgcaag cctgcatgat ttgcgcatcg
     7561 cctgcatgaa atccacaggc aatattggca gaggaaacca gcgttaatag ctctgcgtcg
     7621 ctggcgcagc cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacac
     7681 taattgttcg aaataacgtt gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca
     7741 ctggacaaaa tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc
     7801 aatgatacag gcaatacgcg ggtaaccatc ggtggtctgt gcgtcgttca tcaacacaat
     7861 cggctgcccg ttatgtggca cctgcaccac gcccggtaac aaaccgtgag ataacagttc
     7921 gcgatcggtg gtgcgtttta aaatttgccc ctgtaagcga tagcccatgc ggttactttg
     7981 cgagctaagc tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg
     8041 atattccggc cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg
     8101 cgcttccata aaatcacgct tcgatttgcc aatcgggagt cggtcaccat ccttcagtaa
     8161 acggccttcc agcccgccaa tccccacttt gagatcggtg ctgcatgacc ccattaccgg
     8221 cggaacatca ataccacccg cgaccgccag ataactgcgc atcccgtgct gcgggcgttt
     8281 aagcgttaaa cgctggcctg ctttcatcgg caatcgccag ccggtccaga cggcattatc
     8341 atccagccgc gcttcgcaac cggcacccgt cagagcaaac cacccatcag tttcgaactc
     8401 aaccgttaac tgaccgagcg tgatctccag cgcgggggca ttggcgtcat tacccaccag
     8461 caggttagca atgcgtaacg caggcatatc cagtgcgccg cagtggctga ttcccgactg
     8521 gcgaaaaccg tgacgaccgc catcctgcac agtggtatac atgcccgcac gaataatctt
     8581 cagcatactc cctccttctg cggtacaaag cgcacgctgt ctcccggacg taataagatg
     8641 ggttcgtcac gcgccggatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca
     8701 cccggcgttg ccagcggata aacaccagtc tgcggcccgc cgatcccgac agaacctgcc
     8761 ggaacgagca agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg
     8821 agatacggga agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc
     8881 aattcaacaa cctgtttttc gctaaacccg caatgcgccg cgaccaccgc caaatccggt
     8941 ccgcctgcac caccgtaaac caccggaatt tcaataaagc gagactcagg ttccagggcc
     9001 tcgctctcct cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga
     9061 ttacgcaaaa tcaccgtgat attgttcatg ccgggaatgg cttcaaccac attcggcata
     9121 tccaccagac gctgcgccag tcgccagatc cgtttctggc tagccagcgt caccggcggt
     9181 tccagttcca gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc
     9241 gatccctctg attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc
     9301 ggtattttca ttcagccact cgctcaatgc gcgaatacca ccacgttcag tggcatggtg
     9361 acctgcagca taaaaatgca acccctgctc gcgggctgaa tgaatggtct gttcagaaac
     9421 ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc
     9481 gccgcccgtg caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa
     9541 cggcttacgt cccagacgcg cttcaatcca ggaggctagt tccagtcccg gcacaggcat
     9601 ggtcagctct ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa
     9661 cgccgccagc tgtgcgttat tgcccagctc aggatgtacg tcaagcggca aatgccagcc
     9721 atacaggttg atatcattcg ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat
     9781 gaccggagac tctcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag
     9841 acgcactgcc tcatcgagca aagcctggct ggcggtgaca ccggtaacaa ttttttgcac
     9901 cgtctctttg ccttctacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt
     9961 cagtttttcg ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt
    10021 ctgatagcta caccgttaac atagcgttac ctcgcctgtt cgtcgatagc caatcttttg
    10081 acatattaag aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca
    10141 gctaatgtat atttatgcaa tcagacagca tattgcataa ataatcagat ctgtatcaaa
    10201 gttttaactt taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgcagtcaat
    10261 tgtggaatta ttatgaataa acacgcatca caaccgcgca ctatttacta tgtcgttgcg
    10321 ctgcaaatct gggaatattt tagcttttac ggcatgcgtg ccctgctgat tctctatctc
    10381 accaatcaac taaaatacaa cgatactcac gcctacgagt tatttagcgc ctactgttcg
    10441 ctggtgtatg tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc
    10501 atggcggtga tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt
    10561 gagatccatc cgtcattcct ctatctgtcc ctggcgatta tcgtttgtgg ctatggtctg
    10621 tttaaatcca atgtcagttg cctgctcggc gagctgtatg agccaaccga tccgcgtcgt
    10681 gatggcggtt tctcgctgat gtatgcagcg ggtaacgtgg ggtctattat cgcacctatc
    10741 gcctgtggtt acgcccagga agagtacagc tgggcgatgg gctttggcct ggcggcggtg
    10801 ggtatgatcg cgggtctggt cattttctta tgtggcaatc gtcatttcac acatacccgc
    10861 ggcgttaata aaaaagtact gcgtgcaaca aactttctcc tgcctaactg gggatggctg
    10921 ctggttctgc tggtggcaac gcctgcgctg attaccgtac tgttctggaa agagtggtcg
    10981 gtatacgcct taattgtcgc gactattatt ggcctgggtg tactggcaaa aatttatcgc
    11041 aaagcagaaa accagaaaca gcgtaaggag ctggggctga ttgtgacgct caccttcttc
    11101 agtatgttgt tctgggcctt cgcacaacag ggcggcagct caattagcct ttatatcgac
    11161 cgcttcgtta accgcgatat gtttggttat accgttccga ctgctatgtt ccagtcgatt
    11221 aatgccttcg cagttatgct atgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc
    11281 gcaggtaatc gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc
    11341 gccggattct gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct
    11401 ctgccactga tggtgttagg cctcgcggtg atgggctttg cggaactgtt tatcgacccg
    11461 gttgccatgt cgcaaattac gcgcattgaa attccgggcg tgaccggcgt attaaccggc
    11521 atctacatgc tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag
    11581 acatcgcagg cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt
    11641 gaagtatttg atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt
    11701 tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc ataataggcc
    11761 agcaccgatg cctgatgcga cgcttgacgc gtcttatcag gccggtgatg ctgccaactt
    11821 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct
    11881 gtccctcctg ttcagctact gacggggggg t
//
LOCUS       DAZOXR010000129        11880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZOXR010000129 DAZOXR010000000
VERSION     DAZOXR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.131"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..290
                     /locus_tag="ACHUNN_003050"
     CDS             <1..290
                     /locus_tag="ACHUNN_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0577358.1"
                     /translation="QQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGI
                     SESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            290..5560
                     /gene="traI"
                     /locus_tag="ACHUNN_003051"
     CDS             290..5560
                     /gene="traI"
                     /locus_tag="ACHUNN_003051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR0577359.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5580..6326
                     /gene="traX"
                     /locus_tag="ACHUNN_003052"
     CDS             5580..6326
                     /gene="traX"
                     /locus_tag="ACHUNN_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR0577360.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6381..6941
                     /locus_tag="ACHUNN_003053"
     CDS             6381..6941
                     /locus_tag="ACHUNN_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0577361.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7073..7285
                     /locus_tag="ACHUNN_003054"
     CDS             7073..7285
                     /locus_tag="ACHUNN_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0577362.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7531..7992
                     /locus_tag="ACHUNN_003055"
     CDS             7531..7992
                     /locus_tag="ACHUNN_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0577363.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8038..8247
                     /gene="hha"
                     /locus_tag="ACHUNN_003056"
     CDS             8038..8247
                     /gene="hha"
                     /locus_tag="ACHUNN_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR0577364.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            8285..8875
                     /locus_tag="ACHUNN_003057"
     CDS             8285..8875
                     /locus_tag="ACHUNN_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0577365.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            9030..9503
                     /locus_tag="ACHUNN_003058"
     CDS             9030..9503
                     /locus_tag="ACHUNN_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577366.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            9750..9899
                     /locus_tag="ACHUNN_003059"
     CDS             9750..9899
                     /locus_tag="ACHUNN_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0577367.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            10184..10432
                     /locus_tag="ACHUNN_003060"
     CDS             10184..10432
                     /locus_tag="ACHUNN_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0577368.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            10677..10751
                     /gene="tap"
                     /locus_tag="ACHUNN_003061"
     CDS             10677..10751
                     /gene="tap"
                     /locus_tag="ACHUNN_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0577369.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10744..11601
                     /gene="repA"
                     /locus_tag="ACHUNN_003062"
     CDS             10744..11601
                     /gene="repA"
                     /locus_tag="ACHUNN_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0577370.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT         2904 a         2839 c         3641 g         2496 t
ORIGIN      
        1 cacaacagcc agtgtcttct gtcatcaatg ataagaagtc agatgcaggt gtgagtgttc
       61 cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac
      121 tgccacccgg gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc
      181 aacaggaaaa tcatccggac atccagcagc atatgcagcg tcgtgaagag gtgaacatta
      241 atgtgcaccg ggagcgcggg gaggatgttg agccgggaga tgatttctga tgttgagttt
      301 cagcgttgtt aaatcggcag gcagtgccgg aaactattac accgacaagg ataattacta
      361 tgtgctgggc agcatgggag aacgctgggc cggtcagggg gctgaacagc ttgggctgca
      421 gggcagcgtc gataaggatg tttttacccg tcttctggag ggcaggctgc cggacggggc
      481 ggatttaagc cgcatgcagg atggcagtaa taagcatcgt cccggctacg acctgacctt
      541 ctccgccccc aaaagtgtct ccatgatggc catgctcggg ggggataaac gtcttattga
      601 tgcacataac caggccgtgg attttgctgt ccgtcaggtg gaggcgctgg cttccacacg
      661 ggtgatgacg gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt
      721 taaccatgac accagtcgcg atcaggaccc acagttacac acgcatgtgg tggtggctaa
      781 tgtcacgcag cataacggcg agtggaagac actgagcagt gacaaagtgg ggaaaaccgg
      841 attttccgag aacgtgctcg cgaaccggat tgcgttcggg aaaatatatc agagtgagtt
      901 acgtcagcgc gttgaggcgc tgggttatga aaccgaagtg gtcggtaagc acggtatgtg
      961 ggagatgccg ggcgtaccgg tggaggcctt ttccagtcgt tcacaggcta tcagggaggc
     1021 cgtcggtgaa gacgcctcgc tgaaatcccg ggatgtggcg gcactggata cgcgaaaatc
     1081 caaacagcac gtcgatccgg aaatcagaat ggctgaatgg atgcagacgc tgaaggaaac
     1141 cgggttcgac atccgggcat atcgtgacgc ggcggatcag cgtgcggaga tccgcacgca
     1201 ggcgcccggg cctgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggcgat
     1261 tgccggatta agtgaacgaa aagtgcagtt cacgtatacg gacgtgctgg ccaggacggt
     1321 cggcatactg ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc
     1381 catcagccgt gagcagctta tccccctcga ccgtgagaag gggctgttca catcaggaat
     1441 tcatgtgctc gatgagctgt ccgtccgggc actcagtcgt gacatcatga aacagaaccg
     1501 ggtgaccgta catccggaga aaagtgtccc ccggacggct ggttacagcg atgcggtcag
     1561 tgttctggca caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg
     1621 gcagcgtgag cgggtggctg aactggtcat gatggcccgg gaacaggggc gggaggtgca
     1681 gattatcgct gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg
     1741 tgaactgata accggacgtc gtcagctgct ggaaggcatg gccttcacgc cgggcagtac
     1801 cgttatcgtc gaccagggtg aaaaactctc cctgaaagag acgttaaccc tgctggatgg
     1861 tgccgcacgt cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg
     1921 cagtgcgctg atggccatga aggatgccgg ggtgaacaca tatcgctggc agggcggaga
     1981 acagcgaccg gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc
     2041 cggagatttt gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggggt
     2101 acgggaacag gccatactga cacaggccat tcgcagtgag ctgaaaacgc agggcgtgct
     2161 cggacaccct gaggtgacca tgactgcact gtcaccggtc tggctggaca gccggagccg
     2221 ttatctgcgg gatatgtacc gaccggggat ggtgatggag cagtggaacc cggagacacg
     2281 cagtcatgac cgctatgtta tcgaccgggt gacggcgcag agtcacagcc tgaccctgcg
     2341 ggatgcgcag ggcgaaacgc aggtggtgcg tatttcctcc ctggacagca gctggtcgct
     2401 gttccggccg gaaaaaatgc cggtggcaga cggcgagcga ctgagggtga cagggaaaat
     2461 tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc
     2521 gatgacggtt gttgtgccgg ggcgggctga accggccacc ctgcctgtgg ctgattcacc
     2581 gttcacggca ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagtga
     2641 cagtgcgacg gtgtttgcct ccgtcacaca gatggcaatg gacaacgcca ccctgaacgg
     2701 tctggcccgc agcggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc
     2761 ggaaaaactc gcccgccatc cgtcctttac ggtggtttct gagcagataa aggcgcgtgc
     2821 cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc
     2881 acagcaggcc attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat
     2941 ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttactgaact
     3001 gggaggggaa atcaatgcgc agataaaacg cggtgactta ctgtatgtgg atgtggcaaa
     3061 aggctatggc acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct
     3121 tcgccatatt ctcgaaggca aggaggcggt cacgccgctg atggagagag tacccggcga
     3181 actcatggag acgttaacgt cgggacagcg tgccgccacc cgtatgatac tggaaacgtc
     3241 cgaccgtttc acggtggtac aggggtatgc cggtgtgggt aagaccactc agttccgggc
     3301 ggtgatgtca gccgtgaaca tgctgccgga gagtgagcgt ccccgggttg tggggctggg
     3361 gcccacgcac cgtgcggtcg gtgagatgcg cagcgccggc gtggatgcac agacactggc
     3421 gtcctttctg catgacacgc agctgcagca gcgcagcgga gaaacgccgg atttcagcaa
     3481 cacgctgttt ctgctcgatg agagctcaat ggtgggcaat accgacatgg cacgggcata
     3541 cgccctgatt gcggccggtg gcggtcgtgc tgtggccagc ggtgacacgg accagctgca
     3601 ggccatcgcg cccggtcagc ctttccgtct ccagcagacg cgcagtgctg ccgatgtggc
     3661 catcatgaag gagattgtgc gtcagacgcc ggaactgcgg gaggcggtat acagcctgat
     3721 taaccgggat gtggaaaggg cactgtccgg gcttgagagt gtgaaaccgt ctcaggtgcc
     3781 acgtctggag ggcgcatggg caccggagca ctccgtgacg gagttcagtc acagccagga
     3841 agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa ggcgaggctt tcccggatat
     3901 ccccatgaca ctgtatgagg ccattgtccg cgactatacc ggcaggacac cggaagcacg
     3961 ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg cgcgtactga acagcatgat
     4021 tcatgatgca cgggaaaagg ccggtgagct gggcaaagag caggtcatgg tgcctgtcct
     4081 gaacacagcg aatatacgtg acggagaact gcgtcgtctc tccacctggg agaaaaaccc
     4141 ggatgccctt gccctggtgg atagtgtgta tcaccggatt gccggtatca gcaaggatga
     4201 cgggctgata accctgcagg atgcggaagg taacacgcgg ctgatttcgc cccgggaggc
     4261 ggtggctgaa ggtgtcacac tgtacacccc ggacaaaatc cgggtgggga ccggcgaccg
     4321 gatgcgcttc acgaagagtg accgggagcg cggttatgtg gccaacagcg tctggacggt
     4381 gacagcggtt tccggtgaca gtgtcacgct gtcggacgga cagcagaccc gggtgattcg
     4441 ccccggtcag gagcgggcag agcaacatat tgacctggcc tatgccatca ctgcccacgg
     4501 tgcgcagggg gcgagtgaaa cctttgccat cgcgcttgaa ggcacggaag gtaaccggaa
     4561 acagatggcc ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca
     4621 ggtgtacacc gataaccgtc agggctggac ggatgccatt aacaatgccg tacagaaagg
     4681 aactgcccac gatgtgctgg agccgaaacc ggaccgggag gtcatgaatg cgcagcggct
     4741 gttcagtacg gcacgggaac tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc
     4801 ggggctggcc gggggagaca gtccggcacg gtttattgca ccggggcgca aatatccgca
     4861 gccgtatgtg gcactgcctg cttttgaccg taacggcaag tccgccggta tctggctgaa
     4921 cccgctgacc acggatgacg gaaacgggct gcggggattc agcggtgaag gacgggtgaa
     4981 aggcagcggg gatgcgcagt ttgtggccct gcagggcagc cgtaacggcg agagcctgct
     5041 ggctgataat atgcaggatg gtgtccggat tgcccgtgat aaccccgaca gcggtgtggt
     5101 ggtgagaatc gccggtgaag gtcgtccgtg gaatcccggt gccatcaccg gtggtcgcgt
     5161 gtggggggat atcccggaca acagcgttca gccgggagcc ggaaatggcg aaccggtcac
     5221 ggcagaggta ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg
     5281 cgcagatgaa attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac
     5341 agagcaggct gtcagggaga ttgccggaca ggagcgtgac cggactgcca cttctgaacg
     5401 ggaagccgcg ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg
     5461 ggaggttgcc cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt
     5521 tcgtgacctg cagaaagaga aaaccctggg cggagactga tacaggaaga taaacgctga
     5581 tgacaacgga taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct
     5641 ggttgcagtc atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac
     5701 tggtgctgat ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt
     5761 ttctggccgg gcgaggagcc tttccgctgt ttgccctggt gtgggggctg aatctgtccc
     5821 gtcatgcgca tatccggcaa ccggccatta accggctgtg gggatggggg attattgccc
     5881 agttcgcata ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg
     5941 cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg
     6001 ccattcttct gatggccctg tgggggcctt tgtccggcac cagttacggc attgccgggc
     6061 tgctgatgct ggcagtcagc caccggctgt accgggcgga agacagagcg gaacgtctgg
     6121 cgctggtcgc ctgtctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     6181 cggcggtggc tggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     6241 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     6301 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     6361 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgaa
     6421 aaacggaagg ggagacgcct gtccgcaggc ggaaaaccat catcaatgtc accacgccac
     6481 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcggagctgg
     6541 cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta tctgaacctg ccctcgctgg
     6601 atgaggccgt gaacaccctg aaaccctggt ggccgggatt atttgacggt gaaacgcccc
     6661 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     6721 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     6781 tgtgtgccat gaaagccggt gcctgccggt atgatacgga agggtatgtg acggagcata
     6841 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaaca
     6901 ggataaaggc agagcttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     6961 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg tacccgaaag gaagccaatt
     7021 cagtagatta tttaacacca taatactgac gggttaaaga ggagtgataa agatgaatgg
     7081 attcagaaac agttcacgga acggtcaggt ctggcgctgg cagcgtgccg gaagccgggc
     7141 cgttattctg gaggtcagtg gacgctggat ggaagcggca gaagcatggc gacgggctgc
     7201 cggtgttgcg ccccggacag actggcagca gtttgcccga aaaagggctg aacactgcca
     7261 ccggcgcagc cggggaaggg tgtaacgaaa aaagcagcct tcattactcc agggattcag
     7321 aaggctgaat aatgcatgag atttgctttt tttaatatcc ggtcgggcag tatgaccgga
     7381 cagttctgag tagcacagag ttttttccgt attaagtcgt gattttatat tttgtgatta
     7441 atttcacaaa ataaggtgtt attcagtgtg tgctgcgata ttcaggatta cctgaaatac
     7501 aggtgccatt tatctgatat ggagaataac atgaggaaat atattccact ggtattattt
     7561 atcttttcat ggccggtatt gtgtgcagat attcatggac gggttgttcg tgtactggac
     7621 ggtgatacca tcgaggtcat ggactcacgg aaggcagtgc ggatcaggct ggtaaatatt
     7681 gatgcaccgg aaaagaaaca ggattatggc cggtggtcaa cggacatgat gaaatcactg
     7741 gttgcaggaa aaacggttac cgtcacatat tttcagcgag atcgttatgg tcgtattctc
     7801 gggcaggttt atgcaccaga cggaatgaat gttaatcagt ttatggtccg ggccggggct
     7861 gcctgggtat atgagcagta caacacagat cccgtgctgc ctgtccttca gaacgaagcc
     7921 agacaacaaa aacgtgggct ctggtcagat gctgatccgg ttcccccctg gatatggaga
     7981 caccgtaaat aaagcgatgg caatcttatc attcatctgt tctcagcggg aataaaaatg
     8041 gcgaaaacaa aacaggaatg gctgtatcag ttacggcgat gttcatcagt caatacgctg
     8101 gaaaaaatta ttcataaaaa cagagacagt ttattaaaca gtgaacgtga atcatttaat
     8161 tcagcagcag accaccggtt agcggaatta atcaccggaa agttatatga caggatacca
     8221 aaagagatat ggaaatatgt aaggtaataa ttcagaataa ttaattacca ggagcagata
     8281 aacaatgaag ctgattattt ttattctcat tgttctgatt attgcagcgt tacttatccg
     8341 gattatcctc aaaagtgtaa atcagcattc tcctttgctg atgcagttgc atgcggcagg
     8401 tatacggacc ggtgatgcag agcgaatact gtccggcggt gaatactggc aacgtcagaa
     8461 gacgctgctg acagaaaggg aagtcagttt tatgaaagga ctgttcagaa ttgtggatat
     8521 gaagcggtgg tatctgtgtc cgcaggtacg ggtcgcggat atcgtccagc tgaacgggaa
     8581 tatccggcca cgatcacgcc agtggtggca gttattcagg atggtgtctc agtggcatgt
     8641 tgatgtggtt atcgttgaac ggcgttcgtt cagtattgtt gcagcagtag agctggatga
     8701 tgccagccat ttacgaccgg aacgccggcg ccgggatatt cttctggaag aggttctgag
     8761 gcaggctggt attccgttgc tcagaagcca cgatgccaga aaactgctgc agatgaccgg
     8821 agaatggctg aatacaacag gggctgatca gcagtccccg gaacatcgta gctgacgcct
     8881 tcgcgttgct cagttgtcca accccggaaa cgggaaaaag caagttttcc ccgctcccgg
     8941 cgtttctgta ataaaaagcg ggaaatggtg gtcacagagt aaattccgga accacctgta
     9001 cccaaacttt atataataat ggtgaatata tgaaatatga tgatgtatca gaattgatga
     9061 ttaagcgtga acacaatatt cttattgttg ggaactctgc ctcccacatt gataagtttt
     9121 ttaaggggta ttctatacca ggttctaaat attatgattt cactcagatt aacagtgatt
     9181 cagatgtaga gagaaatgag gatgctgtct cctttatcag ggatgcaatg aatagtgaag
     9241 agcagacaat catttttaat tgtgctgggt ggcctgattt aggtggtgga tcagcagtat
     9301 cacagtttgc aatgatggtc agaaaatttg gtaaacaact tattgtcgct gtaagtgaaa
     9361 aggatgccat caaattaaaa gacaactttg atattattgg gatgttgtca tatggtaaag
     9421 aaaattttat tgccatgagt catacgaaaa acgagttaac tggaggaaaa cgtcggtacc
     9481 ggattaaaaa tggatgtaaa taaaaagcat acttattggc tggagtgacg ccttcgtgct
     9541 tctgtagtaa aaagcgggca atgcaggtaa tagtcagcaa cagggaatgt ggtattatcg
     9601 cggcgggtgt ctgagccttt ctggttcagg caagacgcag gtaccagaaa tgcaaagacc
     9661 ccacttgtta atccattaac tcgtgaggtc tgcatgaagt acattgcact actgattgta
     9721 gcctcttcct tgcagagagg tcaaagttta tgacgaagta tgcccttatc gggttgctcg
     9781 ccgtgtgcgc cacggtgttg tgtttttcac tgatattcag ggaacggtta tgtgagctga
     9841 atattcacag gggaaataca gtggtgcagg taactctggc ctacgaagca cggaagtaaa
     9901 ctgccgggcg gggacggaag tccccgcttt ccggaagtgt gaggtatttc aggggcagac
     9961 acccgacatg ccagaaacag ccggtcccgc ccggggccgg ctcccgggtt aaggcatttc
    10021 ctgttttttc agccatttcg caacaaaaat cacattaaac aacagtaatc cccgttgatt
    10081 tgtgcgccaa cacagatctt cgtcacaatt ctcaagtcgc tgatttcaaa aaactgtagt
    10141 atcctctgcg aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc agacagaaaa
    10201 tgcagtgact tcctcatcag gtacaaagcg agcatacaga aaaggtaatc ctctgacgct
    10261 tgcagaacgt cagcaggcat ccctggcaag aaaacgagct acacataaag aactcagagt
    10321 gttcattcct gctgcgttaa aagcgcaatt gcaggtgatg tgtgaagctg agggcgttac
    10381 ccaggctgag atgattgccg aactgataaa gcagaagagt gcttttagct aactaaaatg
    10441 tagcgacatc acattcttgc ctgtttctgg tgtgcgtgat agattgctga tcgtttaagg
    10501 aattttgtgg ctggccacgc cgtaaggtgg cagggaactg gttctgatgt ggatttacag
    10561 gagccagaaa agtgaaaacc ccgataatct tcgacaagtt tggcgactga gaagattacc
    10621 ggggccatct aaaaaccgta tagccaacaa ttcagctatg cggggagtat agttatatgc
    10681 ccggaaaagt tcaagacttc tttctgtgct cactccttct gcgcattgta agtgcaggat
    10741 ggtgtgactg atcttcaaca aacgtattac cgccaggtaa agaacccgaa tccggtgttt
    10801 acaccccgta aaggtgccgg aacgctgaag ttctgcgaaa aactgatgga aaaggcggtg
    10861 ggcttcactt cccgttttga tttcgccatt catgtggcgc atgcccgttc gcgtggtctg
    10921 cgtcgacgca tgccaccggt gctgcgtcga cgggctattg atgcgctcct gcaggggctg
    10981 tgtttccact atgacccgct ggccaaccgc gtccagtgct ccatcaccac gctggccatt
    11041 gagtgcggac tggcgacgga gtctgctgcc ggaaaactct ccatcacccg tgccacccgg
    11101 gccctgacgt tcctgtcaga gctgggactg attacctacc agacggaata tgacccgctt
    11161 atcgggtgct acattccgac cgatatcacg ttcacatctg cactgtttgc tgccctcgat
    11221 gtatcagagg aggcagcggc cgccgcgcgc cgcagccgtg tggtatggga aaacaaacaa
    11281 cgcaaaaagc aggggctgga taccctgggc atggatgaac tgatagcgaa agcctggcgt
    11341 tttgttcgtg agcgttttcg cagttatcag acagagctta agtcccgggg aataaagcgt
    11401 gcccgtgcgc gtcgtgatgc ggacagggaa cgtcaggata ttgtcaccct ggtgaaacgg
    11461 cagctgacgc gcgaaatcgc ggaagggcgc ttcactgcca atcgtgaggc ggtaaaacgc
    11521 gaagttgagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
    11581 ctggccacag cttccccctg aaagtgacct cctctgaata atccggcccg cgccggaggc
    11641 attcgcacgc ctgaagcccg ccggtgcaca aaaaaacagc gtcgcatgca aaaaacaatc
    11701 tcatcatcca ccttctggag catccgattc cccctgtttt taatacaaaa tacgcctcag
    11761 cgacggggaa ttttgcttat ccacatttaa ctgcaaggga cttccccata aggttacaac
    11821 cgttcatgtc ataaagcgcc agccgccagt cttacagggt gcaatgtatc ttttaaacac
//
LOCUS       DAZOXR010000130        11816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZOXR010000130 DAZOXR010000000
VERSION     DAZOXR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.132"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUNN_003063"
     CDS             <1..389
                     /locus_tag="ACHUNN_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0577371.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            complement(588..938)
                     /locus_tag="ACHUNN_003064"
                     /pseudo
     CDS             complement(588..938)
                     /locus_tag="ACHUNN_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHUNN_003065"
     CDS             complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHUNN_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0577372.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHUNN_003066"
     CDS             2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHUNN_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR0577373.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2916..3704
                     /locus_tag="ACHUNN_003067"
     CDS             2916..3704
                     /locus_tag="ACHUNN_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR0577374.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3910..4257
                     /locus_tag="ACHUNN_003068"
     CDS             3910..4257
                     /locus_tag="ACHUNN_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0577375.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4251..5090
                     /gene="sul1"
                     /locus_tag="ACHUNN_003069"
     CDS             4251..5090
                     /gene="sul1"
                     /locus_tag="ACHUNN_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0577376.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5218..5421
                     /locus_tag="ACHUNN_003070"
     CDS             5218..5421
                     /locus_tag="ACHUNN_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577377.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5577..6782
                     /gene="chrA"
                     /locus_tag="ACHUNN_003071"
     CDS             5577..6782
                     /gene="chrA"
                     /locus_tag="ACHUNN_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR0577378.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6793..7098
                     /locus_tag="ACHUNN_003072"
     CDS             6793..7098
                     /locus_tag="ACHUNN_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR0577379.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7325..8089
                     /locus_tag="ACHUNN_003073"
     CDS             7325..8089
                     /locus_tag="ACHUNN_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR0577380.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHUNN_003074"
     CDS             complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHUNN_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR0577381.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHUNN_003075"
                     /gene_synonym="mrx"
     CDS             complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHUNN_003075"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR0577382.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10401..11306)
                     /locus_tag="ACHUNN_003076"
     CDS             complement(10401..11306)
                     /locus_tag="ACHUNN_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR0577383.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11428..>11816)
                     /locus_tag="ACHUNN_003077"
     CDS             complement(11428..>11816)
                     /locus_tag="ACHUNN_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0577384.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT         2490 a         3391 c         3450 g         2485 t
ORIGIN      
        1 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
       61 tgaagaagtg gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg
      121 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
      181 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca
      241 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
      301 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
      361 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
      421 cgctaacttt gcaacagtgc ccccaatgcg ctgtccagtc atggaattcc ctgcaaaatg
      481 tcagggaaga ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat
      541 cctgacgcaa tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc
      601 ggcgaatcac aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg
      661 aagcgctgat agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg
      721 aaggcggcat cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg
      781 gcgttcatgg tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc
      841 ggcctggcga acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg
      901 cagtcgggtg tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg
      961 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
     1021 gacgggctgc tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga
     1081 tgcggcaccg atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc
     1141 ctacctctca ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc
     1201 aactttcagc acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc
     1261 ctgcacggtt cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag
     1321 ggtgtgcggt gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg
     1381 ctgaaaggtc tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga
     1441 atgcgtgtgc tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt
     1501 ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca
     1561 ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat
     1621 caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc
     1681 cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag
     1741 ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg
     1801 ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg
     1861 cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg
     1921 cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct
     1981 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2041 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     2101 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2161 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2221 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2281 taaaacaaag ttagccatta agggagttaa attgaaaata tcattgattt ctgcagtgtc
     2341 agaaaatggc gtaatcggta gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact
     2401 actctttaaa gcgctcacat ataatcaatg gctccttgtc ggaagaaaaa catttgactc
     2461 tatgggtgtt cttccaaatc gcaaatatgc agtagtgtca aagaacggaa tttcaagctc
     2521 aaatgaaaac gtcctagttt ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt
     2581 tacagatcat gtatatgtct ctggcggggg tcaaatctat aatagcctta ttgaaaaagc
     2641 agatataatt catttgtcta ctgttcacgt tgaagtcgaa ggtgatatca aattccctat
     2701 aatgcctgag aatttcaatt tggtttttga acagtttttt atgtctaata taaattatac
     2761 ataccagatt tggaaaaaag gctaacaatg cgttgcagca ccagtcgctt cgctccttgg
     2821 acagctttta agtcgcgtct ttgtggtttt gctgcgcaaa agtattccac aaagccgcaa
     2881 cttaaaagct gccgctgaac ttaacgttag gcatcatggg tgaatttttc cctgcacaag
     2941 ttttcaagca gctgtcccac gctcgcgcgg tgatcgagcg ccatctggct gcgacactgg
     3001 acacaatcca cctgttcgga tctgcgatcg atggagggct gaagccggac agcgacatag
     3061 acttgctcgt gaccgtcagc gccgcaccta acgattcgct ccggcaggcg ctaatgctcg
     3121 atttgctgaa agtctcatca ccgccaggcg atggcggaac atggcgaccg ctggagctaa
     3181 ctgttgtcgc tcgaagcgaa gtagtgcctt ggcgctatcc ggcgcggcgt gagcttcagt
     3241 tcggtgagtg gctccgccac gacatccttt ccggaacgtt cgagcctgcc gttctggatc
     3301 acgatcttgc gattttgctg accaaggcga ggcaacacag ccttgcgctt ctaggcccat
     3361 ccgcagccac gtttttcgag ccggtgccga aggagcattt ctccaaggcg cttttcgaca
     3421 ctattgccca gtggaatgca gagtcggatt ggaagggtga cgagcggaac gtcgttcttg
     3481 ctcttgctcg catttggtac agcgcttcaa ctggtctcat tgctcctaag gacgttgctg
     3541 ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc cctcatctgc aaggcacgcg
     3601 cggcgtacct gggtagcgag gacgacgacc tagcaatgcg cgtcgaagag acggccgcgt
     3661 tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa
     3721 gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg gcgttcaagc ggacgggctg
     3781 cgcccgccgc tcaactatgc gttagatgca ctaagcacat aattgctcac agccaaacta
     3841 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     3901 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     3961 acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata
     4021 atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt
     4081 gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg
     4141 cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc
     4201 tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg
     4261 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
     4321 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
     4381 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
     4441 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
     4501 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
     4561 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
     4621 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
     4681 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
     4741 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
     4801 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
     4861 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
     4921 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
     4981 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     5041 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt
     5101 ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg
     5161 ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg
     5221 gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg
     5281 cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc
     5341 gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct cctagggatc
     5401 gcctcagaaa acggaaaata aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc
     5461 cacagctaaa actgcgtagt acacatagag gtattttttt ctagatttat atcgaatttc
     5521 gttatcgaat ttcgcttatt attgatcaac tcgttgcaga tcatgaaggc gaaagtatga
     5581 cgaataaccc taccgatgac agcagaccat ggtcggtctt tcttattttt ctgcggcttg
     5641 gattgacatc ttttggcggc cccattgcgc acttgggcta cttccgcgcc gaatttgtca
     5701 cacggcggcg ctggctctcc gaacggagct atgctgactt ggtcgcgctt tgtcagttct
     5761 tgccagggcc tgcaagcagc caggtcggca tagcggtagg actgtctcgg gctggataca
     5821 gcggggcgct ggctgcttgg gctggcttca cgctgccgtc tgccatagcc ttgatccttt
     5881 ttgcgctcgg catctccagc tatggcgatt acgtctcgca gggcgcgttg catggcttaa
     5941 aagtggtggc tgtggccgtg gtcgctcaag cagtatgggg catggcgcgt aacctatgca
     6001 cggatgggct gcgagtcacc atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt
     6061 ccgcgtgggg acaggttggc gtgattgcta tcgcaggcat cgcaggccgg ttattgttca
     6121 agccagcgaa agttgttgag catgaccccc tacctatcac ggtcagtcac cgggccggcg
     6181 tgctttggct ctcgctgttc tttgtcttgc tgattggcct gccggtgttg gccgaactga
     6241 tgccaagtca aaccatggca atggtggatt ccttctatcg tgtcggatca ctggtgttcg
     6301 gcggtggtca cgttgtgctg ccattactgc aagccgaagt ggtgccctcc ggctgggtca
     6361 acaatgaatc ctttctcgcg gggtacgggg cagctcaagc ggtgcccggc cctttgttca
     6421 cgttcgccgc gtttcttggt gcctcgatga acaccgcccc gtcgggctgg atcggcggca
     6481 ttgtgtgtct gctggctatc ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat
     6541 tttgggagcg tttgcgccgc aatacaggca tccaagctgc gctggccggg atcaatgccg
     6601 ctgtagtcgg cttgctgctg gccgcgctgt atcagcctgt atggactagc gccatctttc
     6661 agccgcaaga cttcggcttg gcattagttg cccttgtcgc acttatgttc tggaagctcc
     6721 cgccgtggct ggttgtcctc ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt
     6781 gaggcacaaa aaatgactga caaagacctc tacggcggtt tgatccgcct gcacatcctt
     6841 caccatgcag ccgaggaacc tgtctttggg ctggggatca tcgaagagct acgccgacac
     6901 ggctacgaga tgagcgctgg caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc
     6961 tatctgacct cacgccacga acgcaccggg cgacgtgaac ggcgtgttta tgacatcact
     7021 gaacaaggca gaactgcact tgctgatgcg aaaacaaagg tcaaggagct gttcggagag
     7081 ttggtagaag gtggttgagt ttttgcttct atgtcaggtt gagctatacc ctaactggat
     7141 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
     7201 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
     7261 gtgggctctg ttgcaaaaat cgtgaagctt gagcatgctt ggcggagatt ggacggacgg
     7321 aacgatgacg gatttcaagt ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg
     7381 ctggtattgt cgctatccga tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg
     7441 catttcggtc gaccatacga cgatctatcg ctgggtccag tgctacgccc cggagatgga
     7501 gaagcggctg cgctggttct ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac
     7561 ctacgtcaag gtgcggggca agtggaccta cctgtaccgg gcagtcgaca agcggggcga
     7621 cacgatcgat ttctacctgt cgccgacccg cagcgccaag gcagcgaagc ggttcctggg
     7681 caaggccctg cgaggcctga agcactggga aaagcctgcc acgctcaata ccgacaaagc
     7741 gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac
     7801 ggcccaccgg caggtgaagt atctcaataa cgtgatcgag gccgatcacg gaaagctcaa
     7861 gatactgatc aagccggtgc gcggtttcaa atcgatcccc acggcctatg ccacgatcaa
     7921 gggattcgaa gtcatgcgag ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc
     7981 cggcatcagg ggcgaggtgc gccttgtgga gagagctttt ggcattgggc cctcggcgct
     8041 gacggaggcc atgggcatgc tcaaccacca tttcgcagca gccgcctgat cggcgcagag
     8101 cgacagccta cctctgactg ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct
     8161 cggtgatggt ggagtgaagc caacccgcaa tcgggttatg aatctgcatc gcgattcgca
     8221 atcagctgtc tcttgagcat gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc
     8281 tacaccaact tgtccaactc atccgccaca ccgctcccca ccacctgcga tggccaggtt
     8341 gtttgctggg cgcattggcg gagtatcgtc tcgacccggg acacccacac cggcacaacc
     8401 ttacgggagt gattcactgt caaagaatcg gcccggtgct ctgacgcaag tatcgggatg
     8461 gtcaccattt gtaagccgta gacctgagtg gtgatcaaga cttcgatacc accgaccgta
     8521 ccggtactaa tcgacgacgg tcgtgttcgt cgcctgccgc agggactctg cacacctccg
     8581 tttacgcatg tgcctggagg agttggaaat cgtcgtgttc gggaaacatt aaacacagga
     8641 tggcagcgat ctgagccagc acatgatcag ctagctcacc atccggatcg acggcccact
     8701 gcatcgtcgc gccagcgatg accgagtgca ggagcaactc agctgccgca ggagcacctg
     8761 ggggcagtcg cttgcggatc ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc
     8821 gtagctccgg cacctggagc tcgtaccagg agatgagata gttcaccgag aagtcgttgc
     8881 gagtgttcat gctccgaacg agcacctgca aaaattccca gagcccttgc ggccctgcgc
     8941 ctatcggtat cgcattcagg taatgccgca cctgctcgac gccgcgctcc atcatcctca
     9001 ccagcagcgt atcgcggttg gtgaagcgct ggattaacgc tgcgcgggag agccccacct
     9061 cctttgctac tccgctgagc gtgaactcta tgggaccgca acgcttcagc actacggtgg
     9121 cggcctcgag tacctcgtca tcggacttga gcttggggcg gggcatcagt gttcaccttc
     9181 tgtatgggtt ggggcggagg ctgtggctgc cgccgccatt gtagcaaatt gaagacggag
     9241 cgagagtaga gccacgagcc ccgcaaacac ggccgataca acgaggccag ggagcggacc
     9301 agcaaggtcg acaaacgcgc cggccgcaag cataaccatg ggcgaggctg acagcatcac
     9361 cgccgagacc gtgccgagta cccggccgag aagttctggc ggcgtgcggt tgtagatggc
     9421 agcgttgaga atgggagaga ctgagccggt cagcagtccc acgagcgcgc ccaacaacat
     9481 cagcaccggc acgcctggca actgtgaaag cagaagcgag cccaccgcag agccacaaaa
     9541 tgccaccgcc agccagttct gcgctgatat ccgggcgccg accgacgcat gaatggcaat
     9601 gccaaggaga ccaccagccc ccatcattga ggagaacagc ccgagctctg ctacttggcg
     9661 tcctgcatct acaaacagcg caggcatgat gacgctgccg ttggcgccaa cgatgcccac
     9721 gaagatcatc actataccaa agagagggcg cagcaggggt tcgctccaga gaaaagcgac
     9781 gccggcgcgc atggagagag tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag
     9841 cacccacgcg ccgagcagac ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg
     9901 cgcagcgcca agcaggccga ttgcggcccc cccaagggcc gggccaccta gaatcgcgac
     9961 gttcccgatc accgctttca gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc
    10021 gagtttgggc agctcactgt cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag
    10081 caccacgcaa gcgatcagcc cagtgttcga gagggcgcca acggcatcga gcagtgggat
    10141 gctcgccatg gccacgccgc ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa
    10201 ccgatcgacg aggccaccac caacccacgc gccgatgatg gtcgcgatga cgctgctagc
    10261 ggccgtggcg cccgcccagg ccgcgctctt tgtatgagac aggacgaacc atggaagcgc
    10321 gagggccgcc accgcgttgc cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag
    10381 cgggctatat cgacgttcgc tcattccgct gcggcgagct gcgccttcgc cgcagcgagg
    10441 tactcttcgt tacccgagtc gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg
    10501 cgctccgcga tgtggtgggc gagccgcggc cacacccggc caccggccgc ttcatacgtg
    10561 aggaggagct tcgcgagccc ctcttcacca aagaccataa ggtgcgcggc catgtcgatg
    10621 gcagggtcat caacgcgggc ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg
    10681 tcgatgagca catggcccac gtagagatcg ccatgcacca ccacggagaa atctggccac
    10741 gacgaatcgt cgtcgagcca gcgctgccac cggtggaggc gcttgtcgtt caccacgaac
    10801 tcgcgtcgga cgcggtcaac gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg
    10861 atgagcatcc ccgcatccac ggcggcggaa atggggacgg catgcagggc ggcgagcgcg
    10921 gtcgcgaagc tctccgcgaa gacctccgag tcctgcggca cgacccagtc gggcgtggac
    10981 gaaccaggct ggatgaccat cgcagtcgag tcttcgagca tgggataggc aacgagctcg
    11041 gcgttggcca cgcgccagtc cggcaccgcg aacggcaggc gattcttgag cattgccagc
    11101 acccgcgcct ctggttcgac cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc
    11161 caccgacgtc catcgtcgac ggtggcgatc acgatcctat agtcgagccc aagctcattg
    11221 acagtcagcg ggccatggag cttgagccca tgtcgggctg caagtgcgta cagttgggag
    11281 gtatcggcgg tcgtgactac ggtcatgatt cactcctgag ggcttgacgg gtttagccac
    11341 ctaaatgtaa cagtcacgtc ggttatattc aatccggcac tgttgcaaag ttagcgatga
    11401 ggcagccttt tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat
    11461 ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat
    11521 cacctcaata cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc
    11581 gccgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat
    11641 ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg
    11701 accataggcg ggcgctttat ccgtgttgat gaatcgcggg atctgccact tcttcacgtt
    11761 gttgaggatt ttacccagaa accggtatgc agctttgctg ttacgacggg aggaga
//
LOCUS       DAZOXR010000131        11807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZOXR010000131 DAZOXR010000000
VERSION     DAZOXR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.133"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..487
                     /locus_tag="ACHUNN_003078"
     CDS             <1..487
                     /locus_tag="ACHUNN_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0577385.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUNN_003079"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUNN_003079"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0577386.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUNN_003080"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUNN_003080"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0577387.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUNN_003081"
     CDS             complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUNN_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0577388.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUNN_003082"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUNN_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0577389.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUNN_003083"
     CDS             complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUNN_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0577390.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUNN_003084"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUNN_003084"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR0577391.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUNN_003085"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUNN_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR0577392.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="ACHUNN_003086"
     CDS             complement(6838..7746)
                     /locus_tag="ACHUNN_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0577393.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUNN_003087"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUNN_003087"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR0577394.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUNN_003088"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUNN_003088"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR0577395.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11807
                     /locus_tag="ACHUNN_003089"
     CDS             10714..>11807
                     /locus_tag="ACHUNN_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0577396.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3005 a         2999 c         2927 g         2876 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg
    11521 ctacttttta ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat
    11581 ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt
    11641 ctttttttct ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag
    11701 gcggatttac cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg
    11761 gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacat
//
LOCUS       DAZOXR010000132        11780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZOXR010000132 DAZOXR010000000
VERSION     DAZOXR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.134"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..612)
                     /locus_tag="ACHUNN_003090"
     CDS             complement(<1..612)
                     /locus_tag="ACHUNN_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0577397.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRF"
     gene            complement(735..2381)
                     /gene="fumB"
                     /locus_tag="ACHUNN_003091"
     CDS             complement(735..2381)
                     /gene="fumB"
                     /locus_tag="ACHUNN_003091"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0577398.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="ACHUNN_003092"
     CDS             complement(2459..3799)
                     /gene="dcuB"
                     /locus_tag="ACHUNN_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0577399.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="ACHUNN_003093"
     CDS             complement(4370..5089)
                     /gene="dcuR"
                     /locus_tag="ACHUNN_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0577400.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5086..6717)
                     /locus_tag="ACHUNN_003094"
     CDS             complement(5086..6717)
                     /locus_tag="ACHUNN_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0577401.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6898..7128
                     /gene="yjdI"
                     /locus_tag="ACHUNN_003095"
     CDS             6898..7128
                     /gene="yjdI"
                     /locus_tag="ACHUNN_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0577402.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7140..7412
                     /locus_tag="ACHUNN_003096"
     CDS             7140..7412
                     /locus_tag="ACHUNN_003096"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0577403.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7639..7935
                     /gene="ghoS"
                     /locus_tag="ACHUNN_003097"
     CDS             7639..7935
                     /gene="ghoS"
                     /locus_tag="ACHUNN_003097"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0577404.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7963..8136
                     /gene="ghoT"
                     /locus_tag="ACHUNN_003098"
     CDS             7963..8136
                     /gene="ghoT"
                     /locus_tag="ACHUNN_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR0577405.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="ACHUNN_003099"
     CDS             complement(8255..9772)
                     /gene="lysS"
                     /locus_tag="ACHUNN_003099"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0577406.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="ACHUNN_003100"
     CDS             complement(10009..11466)
                     /gene="dtpC"
                     /locus_tag="ACHUNN_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR0577407.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11525..>11780)
                     /locus_tag="ACHUNN_003101"
     CDS             complement(11525..>11780)
                     /locus_tag="ACHUNN_003101"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0577408.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3045 a         2856 c         2892 g         2987 t
ORIGIN      
        1 aaagcgccgt agctggcggc aatgaaagcg agcgaggaat atcaccgtcg ttaatgcgcc
       61 aagcagcgcg cagaacatat cggattgcgt gtcccactgg tcgccctggg tgccgagaaa
      121 atcatccgct ccctggccca ttgccagcgc cgcccaccac tcgattaatt catacatcgc
      181 gcttatcgcc agcgccacgc agcagactag gaacgccacc attttacgtc cgcgcacgta
      241 catcccgcgc acgagaattt ctcgtgccac cagtgcaggc accagcccct ggaaaaaatg
      301 ccccagcttg tcatacggat tacggctcaa ccccagccat tcctgcacct caaaaccgat
      361 ggggactttc gcgtaggtgt attgcccgcc gaccatcagg atgatggcgt gaaagaaaat
      421 gagcgtatag agcagcggcg ttaacggata acgtttagcg gtggcaagca gtagcggcac
      481 gacaataatc accggtgtca cttccatcag ccaggtgagt ttgtcatggg ccgaaatgcc
      541 ggtataaatc aggattaacg ttagcgccag tgcgccggtg ttaagaatta acggtttgag
      601 tgtgcgggtc atggtgagtt cgcaagtcag ggaaaatact gactattcac cagcaagcat
      661 gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc tggcagcatg ctgccaggcg
      721 ctgggccgaa gaggttactt agtgcagttc gcgcactgtt tgttgacgat ttgctggaag
      781 aagtcgttac ctttgtcatc gaccaggata aacgccggga aatcttctac ttcgattttc
      841 cagatagctt ccatacccag ctccggataa gcgacgcatt ccagatgctt gatgctctgc
      901 tgcgccagta ccgccgccgg accgccgatg ctaccgaggt agaagccgcc gtgtttatga
      961 cacgcgtcgg taacctgctg gctgcggttg cctttcgcca gcatgatcat gctgccgccg
     1021 tgggattgca gcagatccac ataggagtcc atacgacctg cggtggttgg gccaagtgaa
     1081 cctgatggat aaccggcagg agttttcgcc ggacccgcgt agtagatcgg gtgatctttg
     1141 atgtactgcg gaagttcttt accggcgtca atcagctctt tcagcttggc gtgtgcaata
     1201 tctcgaccca caataatggt gccagtgagc gacaaacgag tggaaaccgg atattgcgaa
     1261 agctgggcga ggatctcttt catcgggcgg ttaaggtcga ccttcaccgc ttcgccttca
     1321 ccggcctgac gcagttcttg tggaatgtac tgacctggat tgtgttccag tttttcgatc
     1381 cagatacctt cgcggttgat tttcgcttta atgttacggt cagcggagca ggagacgccc
     1441 atgcctaccg ggcaggatgc gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg
     1501 aagtatttac cgccaaactg cgcgccaaga ccgagtttct gggcctcttc cagcagttcc
     1561 tgttccagct ggacatcgcg gaacgcctga ccatgttcgt tcccttccgt cggcagttca
     1621 tcgtaatagt gagcgcttgc taatttgacg gttttcaggt tggtttccgc agacgtaccg
     1681 ccaatcacaa acgcgatatg gtacggcggg caggctgcag taccgagggt acgcattttc
     1741 tcgacgagga agtttttcag tttgccggga gtcagcaggg ctttggtttc ctggtagaga
     1801 tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca
     1861 tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc cggtgttgac ctctttgtac
     1921 atgtccagcg ccgcgttttg cgagtagcgc aggttatctt cgatataggt gttatagacg
     1981 cctttcgaca gcgcttcttc atcaccgcca ccggtccaca cgcgctggcc ttttttaccg
     2041 acgatgatcg cggtgccggt atcctggcag gtcggcagca cgcctttggc ggcgatttcg
     2101 gagtttctta agaattgcag tgccacgtac ttgtcgtttt cgctggcttc tggatcgtga
     2161 agaatagccg caacctgttt ctggtgtgcc ggacggagca tgaaagaagc gtcgtgaaag
     2221 gcttgctgcg ccagcagggt cagggcttct ggttccactt tcaggatggt ttcgccgtcg
     2281 aagtcggcaa cgctaacgta atcggaagtg agtagatagt attcggtatt gtctttcccc
     2341 atcgggaaag gtgcctggta gataaagggt ttgtttgaca tagcttccag cctgtaactc
     2401 tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt
     2461 ataagaaccc gtacatcgcg gcgaagatcc agccgaagac gcacgatacg ctcacaccaa
     2521 tcaaccccgg cagaataaag ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg
     2581 aacggtcaaa ctgaatcgct gccagatcgc tcggataagt cggcaggatg taataaccgt
     2641 agcaagccgg tgctgaagcc acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg
     2701 gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa cttggaaacc agcagcagaa
     2761 caatggcata ggcccacgga tactctttca ccatttcacc cagtacgccc tgaatttcag
     2821 acatatgcgc accgaacatg gtttctgcca tccatgcgat accgtacacc gccacgatgg
     2881 cgatcatacc ggaacggaag acttcgtttt ttgagataga cgcgggattg gttttggtca
     2941 ggataataat cagcgccccg gtcagcagca taaacatctg aataaccagt accatcgaca
     3001 gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc accaagaagg gcgactacag
     3061 cgattgcccc gaggaaaatc cacattgcca gccagttgct tttcggcagt tttttgtcca
     3121 gcagcgtcgc ggtatcaccg taaacatact cacggttttc cggtacggag atgaatttct
     3181 ggaactcttc gtctttatcc agatctttac cacggaacca gctgaagata ccgatcgcca
     3241 ggataccgat taacgtcgat ggaatggtga ttgccagcag gtcgaggaac tcaagatggc
     3301 gaccatcaaa ggtgacatta cccagcattg caaccagaga cacgaccgca accgacaccg
     3361 gactggcgat gatccccatc tgtgcaccga tagaacttgc cgccatcgga cgttccggac
     3421 ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag aatggtgtaa accacatgac
     3481 ccgtaccgca aagaatggtc agcgtacagg tcacaaacgg cgcgacaatt gagacatatt
     3541 tcgggttgcg gcgcagcagc ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag
     3601 cttgcaaggt cgccgatgcc gccaccaccg caatgataac cagcatgaca tcaaccggtg
     3661 gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac cagaccgata ccgcctaata
     3721 aacccagcgc gataccaccc tttctggcac cataaaacag acatatcagt attatgataa
     3781 gttggatagt aaataacatg tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa
     3841 tagatcacag tcacgttctg ttttgtatga actgttttca gagcctgact aaatatccgt
     3901 ttggtcgtta ccggctttag caaatacctc acagtgaata ttggctgatt aaagcagcgg
     3961 caaatttatg cactgtgata agcggctttt tcagagagaa agactctctg gcgatgggtt
     4021 tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg ttatcttatt gatatttatc
     4081 ggtctatttt ttgttctcag ggcagatgtc attaggttta tagattaatc tgatctaccc
     4141 atttgtgggt aaaaatacac ataatgcggg tgacataata gttaattaac ttttgttagc
     4201 gttttgaaat taaaaacacc gttcacctga agagatatta atttttagcg atgatgcagg
     4261 gataattata tttgatctgg cacaagtttt actgatgaag gatgtaactt gtgccagggg
     4321 tattttgcat tacggtaata attattactt acagataacc agcgatccgt tattggcaat
     4381 attgtttcag tagtgagtag tgttctgcct gaatacggta acggtaaact ggacgccccg
     4441 tgacgccata atggatactg gtgaacaaga tgtggcagtt gaccagccag atgaggtatt
     4501 tacggcagga aacacgcgaa atgttaacct cgttggctag ctcgtcggtt gaaaattcat
     4561 agtcctgatg cgcgtcaatc cactggcaca gtgtgcgtaa cgtctgcggc gttaagcctt
     4621 ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc gtggattagc tgatcaagct
     4681 cggcctggtc ataatactga tgtttttcca gcgccatttt cttttgccgc cagtcggtga
     4741 gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag gtaatccacg acaccgtaat
     4801 gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga gatgacaatc acatcacttt
     4861 tgcaacgcgc gttatgcagg acaggcagta aatcgagccc gttctctttt tgcatataga
     4921 tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa gataatctct ttggctttct
     4981 ccagcgtcga ggctgttcca cagcattgaa agcctgggat ttgtgctacg tatcggcgat
     5041 tcagctccgc gaccattgcg tcgtcatcga taattaatac attgatcatc tgttcgacct
     5101 ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa atcccgggtt ccgattccac
     5161 ggcgatgctg ccgccgagat tttctacctg ttgtttgaca agtgctaaac cgacgcctcg
     5221 ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat
     5281 ccccggtcca tcatcgttaa cttcgcagtg cagccagccg tgacggtagt gcaatgttac
     5341 gctaatttcg ccaccgggtt ccggccctaa tgcttccagc gcgttttcta tcagatttcc
     5401 caatgtggta atcagcgtcg cgacctggtc ctcactgccg ctgtctggca gctggctttc
     5461 actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcga ttaatcttgc tgattaaaaa
     5521 accagcgata accggagatt tgatcttacc cagcagagag ccaatctctt cctgatagtt
     5581 attggctgtt tttagaatgt aatcttccaa ctgcttataa ctcttcagat gcaataatcc
     5641 gagaatcacg tgcaatttat tcataaattc gtgggatcgt tcacgaagtg cgtcggcata
     5701 gttgaccaga ccgtcgagtc gctgcatcag tttacgtact tcggttttgt ccctgaaggt
     5761 tgaaatggca ccgatgataa cgccattact gcgcaccgga acggtgttga tcagtaatag
     5821 ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga
     5881 gacatctacc acctgtgacc atgagtggct gagcgtcgac agtttctcat cgtcctgcga
     5941 cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc
     6001 gtccacggca acgacgcctt ctttgataga ctgcaacatg gcctggcgtt gctcaaacag
     6061 cgtggagatt tcgtagggtt ccaggccgaa aaggattttt ttcagtacct taaccagaat
     6121 gcaggtgcca atcagtccga ccagcatgcc aaataatacc gaccagataa tgctccagcg
     6181 actgtcattg atttgttggg tcacgcggct taactcaagg ccgatcgcca ccacgccaat
     6241 ttgtttatga ttttcatcgt agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc
     6301 gcgattgata gcgacatttt ctttgccatt cagcgcttta aggatgtcat cacctttaaa
     6361 tggctgacca atacgctggg cttcaggatg cgagtagcga agactttgca tatcggtaac
     6421 gacaataaac agcagatcgt tgcgtttgcg cacggcttcc gcgatggcct ggatgccgct
     6481 ctcctgcggt tttttctgca agccctgacg gatttccggc gagttggcga gggtacgcgc
     6541 cactgccagt gccttgttgg ctagcccgtc tcgcgtcata tcactgattt gcgagaagta
     6601 aatcagatgc accaccaata gcaccgagaa cagtaccgca ctgaccatta agatcactgt
     6661 ggtactcaat ttcatcggac gtttgcgtaa catgcggtag ggcaatgaat gtctcatcag
     6721 cttccttgtg tgacaaattt cttaaacatt atctctgatg aggtgggtaa ttcaaaggga
     6781 gtaagaatga ttggctatat aggggaagag attctggcaa cggaaactgc cagtgctgta
     6841 tgaagattcc agggctatgc ttatagcgat aatcatactg atgagagagg gaaagtcatg
     6901 gatcaggcgc tactggacga gggttatcgc tgttataccg gcgaaaagat cgatgtctat
     6961 ttcaacactg cgatatgtca gcattctggc aattgcgtac gtggcaacgg caagttattt
     7021 aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg acgtcgccac tgtggttaaa
     7081 gtgattgata cgtgcccgag cggcgcgctg aaataccgtc ataaataagc gagggtaaaa
     7141 tggaaatacg cgaaggccac aataaatttt acattaatga cgaacaaggc aagcaaatcg
     7201 ctgaaattgt ctttgtgccg actggagaga atttagcgat tatcgaacat accgatgtcg
     7261 atgaaagcct gaaagggcag gggattggta aacagctggt tgcaaaagtc gtggaaaaaa
     7321 tgcgtcggga aaaacgaaaa attatcccac tatgcccatt tgcgaaacac gaatttgata
     7381 aaacgcggga gtatgatgat attcgcagtt gatgggagag tacagagtca cgatattttt
     7441 catgctctcc gcggagtgat gcaggagagc atctgaaggg tagggggatg cacaaagaat
     7501 gggcagagag cgcgtttttt tgtcccaagt catccccttt actgagcaaa aaaaagaata
     7561 tctcctatat gagaatcatc aatcggggtt atcaagtttt gcgtccccag agcattcaat
     7621 attgatagga gtcatattat ggaaggtaaa aacaagttca atacttatgt tgtttctttt
     7681 gattatccat catcttattc ctcagtgttc ttaaggttaa gatcattgat gtatgatatg
     7741 aatttctctt ctatcgtggc tgatgaatat gggataccac gacaattgaa tgaaaattcc
     7801 ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag atttaatcag gcttaaatgc
     7861 ttagacttac cggatattga ttttgacctc aacattatga cagttgatga ctatttccgt
     7921 cagttttaca agtagccagg aaggataaga agaacataaa aaatggcact attctctaaa
     7981 atattaattt tttatgtgat tggtgtgaac atatcctttg tcattatctg gtttatctca
     8041 catgagaaaa cacatattcg tttacttagt gcattcctgg tcggaataac ctggccaatg
     8101 agtctgcctg tggcattact tttttctctc ttttaggagc tgtagttgag tggacccgct
     8161 ggttatcgtg aaacgaagcg tctctcctca aaactggatt tgcggaacga cgaacgaagc
     8221 agtcgcctga ctgcttcgtt cattaaagtg aaatttattt ctgtgggcgc atcgccggga
     8281 agagaataac gtcgcgaata gtatgactgt ttgtaaacag cataatcatt cggtcgatac
     8341 caatacccag accagcggtt ggcggcagac catattccag cgcagttacg taatcttcgt
     8401 catagaacat ggcttcgtcg tcacctgcag ctttagcgtt aacctgttcc tggaaacgtt
     8461 cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt accgatttca cgaccaccga
     8521 tgaagaattc aaaacggtcg gtgatttccg ggttaacatc attacggcgc gccagcggtg
     8581 acacttctgc cggatattcc gtaataaagg ttggctgaat cagatgtgct tctgccactt
     8641 catcaaagat ctccgtgata atacgtccca gcccccagct tttctctacc gtaattccga
     8701 tagattcagc taatgcttta gcagcatcaa aattatccag gtcggccata tcggtttctg
     8761 gacgatattt tttgattgct tcgcgcatgg tgagtttttc aaacggtttg ccgaaatcaa
     8821 acacatgctc gccataagtg actttagtgg tacccagaac ctcttgtgcc agggtgcgga
     8881 acagtgactc tgtcagttca atcaaatcgt ggtaatccgc atacgccatg tagagttcca
     8941 tcattgtgaa ctcaggatta tggcgaacag aaataccttc attacggaag ttacggttga
     9001 tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt cagatacagc tccggcgcga
     9061 tacgcagata catatctaaa tccagagcat tatgatgggt aataaacggg cgagcagatg
     9121 ccccacctgg aattacctgc atcatcgggg tttctacttc cataaagccg cgcgcgacca
     9181 tgaactgacg gatagcggcc agaatttttg aacggacaac aaacgtttga cgggatttat
     9241 cgttagcgat gaggtccaga taacgttgac gataacggac ttcctgatcc tgcagaccat
     9301 ggaatttatc tggtaaagga cgtagtgctt tagtcagcag gcgcaactca gtacagtgaa
     9361 tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg ggcaccgata atgtcaccca
     9421 gatcccattt tttaaactga tcgttataaa caccttctgg caggctatct cttgcaacgt
     9481 acagttgaat acggccaccg acatcctgca acgttacaaa ggaggctttc cccatgatac
     9541 gacgggtcat cattcggcca gcaaccgaga cttcaatgtt taaggattcc agttcctggt
     9601 tattcttcgc atcaaactct tcgtgcaact ggtcagaggt atggtcgcgg cgaaaatcat
     9661 tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt ttcgcggcga tttctcagtt
     9721 catcgttaaa atcaatagcc tcattggctc cccgtgtttc ttgttcagac attttggttc
     9781 ctctaaatcc agctttcaat ttagcgtaga taaagagaca gatcggtaca ctgtattatc
     9841 tgcctcgact attaataact cagtttatct tatcgaaact aacgcttacc tcaagttgat
     9901 atcactaata aataaccatc aaaatggttt aaatgactta ttaatctcga tttactagtg
     9961 tgattaactg aaagttaatt tattcataac caattgtttt tacgacagtt aatcgttgct
    10021 ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg gtagcaaagg ccaatacgac
    10081 gatgatcgcg acacaagcca acgtccactc tcccatctga gaaaagaatc gctggtaagc
    10141 tgcaattacc ataccgctaa tttgcgactc tgtcgtctgc tgtgccacga cgcctgccag
    10201 ccagttggcg accgcgcctg ttgccagcat ataaataccg gttaatacgc cagacatttt
    10261 cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag agttctgcaa agcccattag
    10321 cgccaacccg gatatcatca cgcccattga cgcttgaccg tcagccgctg catgtcgggc
    10381 atcaaatgcc aacaacataa agccacaagc catcagcagt aagccaaagg caaacttcag
    10441 ccagacgcgc aatgttgagt tgccgcggct ttctggcgac gccagccagg ccagtacaac
    10501 cccagcgagc atcactgcaa ttgcattcac cgactggaat agtgctgtag gtacttcaat
    10561 attgaatgcc tgacgattca caaagcgatc gataaacaag ctgatggtac tgccgccctg
    10621 ttgtgccagt acccagaaca acgtcccgac aaacatcaac aatacaattt gccaaagagc
    10681 acggcggtgt tcggggaatt tgatcatcat gcgggcaatg atttgtgcgg caatgaggca
    10741 aacgatcgcc agcaaatatc cggaccagtc gttctccagc agcagggtaa aaaatactgg
    10801 ggctaaacag agcatcacca ctaaccagct ccatactggt aaggcaaatt tgacgctagt
    10861 gagcgctttt ttatccatac tacgcgtgga ctggaaatga cgatgaccgc ttaagaaaat
    10921 caacaaaccg ataaacatgc cgccacccgc aagggcaaag ccaacatgcc atccatacca
    10981 ctgagcagcc aggccgcagg cgattggggc ggcgatagaa ccgatattgc ccgcagcata
    11041 cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg tcgtagagct cgccaagcaa
    11101 acagctgatg tttgatttga ataaaccgta gccacaaata atgattgcca gcgccagata
    11161 caggctaaag gttgaatttg tatcaatgcc cagcaccaca tggccaaggg tcattaacag
    11221 cgcgccggca atcactgcag tgcggttgcc gagcaggcgg tcggcaagcc agccgccgag
    11281 aataggggta acgtaaacca gagaagcata tgcgctgaac aggctgatgg catggttatc
    11341 atcaaaacca agctgatggg tgagatagag aatgagtaag gcacgcatgc cgtaaaaact
    11401 gaagtactcc cagatttgga tcgccacgat atagtatatc gcgcgcggct gtgagggtgt
    11461 tttcatgtgt tctccttatg agcaaaaaag ggaagtggca agccacttcc cttgtacgag
    11521 ctaattattt tttgctttct tctttcaata ccttaacggt atagcggcca tcagcctgac
    11581 ggtatgcacc gtgaatatcg gtttcaaatc ccggatagtg agtgccgatt tcacacagca
    11641 tctgcaggaa ctccagaacc ggacggcttt cttcggtgat catttcaccc ggcattacca
    11701 gaggaactcc cggcggatac ggaaggatca tattggcgtt aatacgacct accatttcgt
    11761 cgaggtaaac ttcttcggtc
//
LOCUS       DAZOXR010000133        11705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZOXR010000133 DAZOXR010000000
VERSION     DAZOXR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.135"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            305..1492
                     /gene="ygeW"
                     /locus_tag="ACHUNN_003102"
     CDS             305..1492
                     /gene="ygeW"
                     /locus_tag="ACHUNN_003102"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR0577409.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            1550..2746
                     /gene="dpaL"
                     /locus_tag="ACHUNN_003103"
     CDS             1550..2746
                     /gene="dpaL"
                     /locus_tag="ACHUNN_003103"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR0577410.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            2804..4015
                     /locus_tag="ACHUNN_003104"
     CDS             2804..4015
                     /locus_tag="ACHUNN_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR0577411.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            4068..5452
                     /gene="hyuA"
                     /locus_tag="ACHUNN_003105"
                     /pseudo
     CDS             4068..5452
                     /gene="hyuA"
                     /locus_tag="ACHUNN_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            5500..6432
                     /gene="arcC"
                     /locus_tag="ACHUNN_003106"
     CDS             5500..6432
                     /gene="arcC"
                     /locus_tag="ACHUNN_003106"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0577412.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6473..8098)
                     /gene="yqeB"
                     /locus_tag="ACHUNN_003107"
     CDS             complement(6473..8098)
                     /gene="yqeB"
                     /locus_tag="ACHUNN_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR0577413.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(8146..8916)
                     /gene="yqeC"
                     /locus_tag="ACHUNN_003108"
     CDS             complement(8146..8916)
                     /gene="yqeC"
                     /locus_tag="ACHUNN_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR0577414.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            9019..9597
                     /gene="mocA"
                     /locus_tag="ACHUNN_003109"
     CDS             9019..9597
                     /gene="mocA"
                     /locus_tag="ACHUNN_003109"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR0577415.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            9919..>11705
                     /gene="ygfK"
                     /locus_tag="ACHUNN_003110"
     CDS             9919..>11705
                     /gene="ygfK"
                     /locus_tag="ACHUNN_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR0577416.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         3058 a         3077 c         2819 g         2751 t
ORIGIN      
        1 gcggtgagtt tatcaaactg aagagagata gcctgcccct ttatcttatt tctgatactt
       61 agcagaaaat aaataacgcg ataaaaaaag ccaaacgttt tcgtatttta caaacaacca
      121 gaagctggca tcaatttgtg aacaacccca cacattatcc gtcaaattag tcttttgcag
      181 ccgcgcggat aattctggca cacttattgt tagtcccagg tatagcaatg aaaacaccaa
      241 tcactttggc aagtcacagt gaaataaacc actttgcctg tcattccact accgggactg
      301 tatgatgaaa actgttaatg agctgattaa ggatatcaat tcgctgacct ctcaccttca
      361 cgagaaagat tttttgttaa cgtgggaaca gacgccagat gaactgaaac aagtactgga
      421 cgttgccgca gcattaaaag cactgcgtgc tgaaaacatc tcaaccaaag tctttaatag
      481 tggattaggt atttccgtat tccgcgacaa ctccacccgt acccgcttct cttatgcttc
      541 cgcgcttaac ctgctcggcc ttgcacaaca agatctcgac gaaggcaaat cacaaatcgc
      601 tcacggcgaa accgtgcgtg aaaccgccaa tatgatctcc ttctgcgccg acgctattgg
      661 tattcgcgac gatatgtatc tgggcgcagg caacgcctat atgcgtgaag ttggcgctgc
      721 acttgatgac ggttacaagc agggtgtact gccacagcgt ccggctttag tgaacctgca
      781 atgcgatatt gaccacccga ctcagtcaat ggctgacctc gcgtggttac gtgaacactt
      841 tggttcactg gaaaacctga aaggtaaaaa aatcgccatg acctgggcct actctccaag
      901 ctatggcaaa ccgctctctg taccacaagg catcatcggt ctgatgactc gcttcggtat
      961 ggatgtcacc ttggcccatc cggaaggcta cgacctgatc ccggatgtgg ttgaagtggc
     1021 gaaaaacaat gctaaagcct ccggtggtag cttccgtcag gtcaccagca tggaagaagc
     1081 cttcaaagac gcagacatcg tttatccgaa gtcatgggca ccttacaaag tgatggaaga
     1141 gcgtactgaa ttgctgcgtg cgaacgacca cgaaggctta aaagcactgg aaaaacagtg
     1201 tctggcacag aacgcgcaac acaaagactg gcattgtact gaagagatga tggaactgac
     1261 ccgtgatggc gaagccctgt acatgcactg cctgccagct gatatcagcg gcgtatcctg
     1321 caaagaaggt gaagtgactg aaggcgtatt cgaaaaatac cgtatcgcta cctacaaaga
     1381 agccagctgg aagccttata tcatcgccgc gatgatcctg tcccgtaaat acgccaaacc
     1441 aggtgcactg ctcgagcaac tgctgaaaga agcgcaagaa cgcgtgaaat aacccctcgg
     1501 gccgatctgc tggtcggccc tctatttcca gaggccaaaa ggataggata tgtccgtttt
     1561 ctcattgaag attgatatcg ccgataacaa atttttcaac ggcgaaacat caccgctctt
     1621 ttcgcaaagc caggccaaac tggcgcgcca gttccaccag aaaatagctg gttatcgccc
     1681 aacaccgctt tgtgcgctgg acgatctcgc aaaccttttt ggtgtgaaga aaattctcgt
     1741 caaagacgaa tcaaaacgat tcggtctgaa cgccttcaaa atgcttggcg gtgcgtacgc
     1801 catcgctcaa ttattgtgtg aaaaatatca tcttgatatc gaaacgctgt catttgagca
     1861 cctgaaaaat gccatcggcg aaaaaatgac tttcgcgacc accaccgacg gcaaccacgg
     1921 gcgcggtgtg gcgtgggcag cacagcaact cggacagaat gcggtgattt acatgccgaa
     1981 aggttctgct caggaacgcg ttgacgccat tctgaacctc ggtgccgagt gcatcgtcac
     2041 ggatatgaac tatgacgata ccgttcgcct gaccatgcaa cacgcgcagc agcacggctg
     2101 ggaagtggta caggacacgg catgggaagg ttacaccaaa atcccaacct ggatcatgca
     2161 aggctacgca accctggcag atgaagccgt cgagcaaatg cgtgaaatgg gcgtaacccc
     2221 gacgcacgtt ctgctgcaag ccggtgtcgg agcaatggcc ggtggtgtgc tgggttatct
     2281 ggtcgacgtc tacagcccgc aaaatctgca cagcattatt gttgaacctg acaaagctga
     2341 ctgtatttat cgctccggcg tcaaaggcga catcgtcaac gttggcggtg atatggccac
     2401 catcatggcg ggcctggcct gtggcgaacc taacccgctg ggctgggaaa tcctacgtaa
     2461 ctgcgccacc caattcatct cctgccagga cagcgttgcc gcattaggta tgcgcgtgct
     2521 gggtaatccg tacggcaacg acccgcgcat catctccggt gaatccggcg ctgtcggttt
     2581 gggcgttctc gcagcggttc attatcaccc gcaacatcaa agcctgatgg aaaaactggc
     2641 gctggacaaa gatgccgtag tgctggttat cagcactgaa ggcgacaccg acgtgaagca
     2701 ctaccgcgaa gttgtctggg aaggcaaaca cgctgtagca ccttaatcac cctattggaa
     2761 ctactccctg caaaacgggg agtaaaaaat ctggagaaaa ataatggcta agaatattcc
     2821 attcaaactg attcttgaaa aagcaaaaga ttaccaggcg gacatgactc gcttcctgcg
     2881 cgacatggtt gctattccca gtgaaagctg cgacgaaaaa cgcgtagtac atcgtattaa
     2941 agaagagatg gaaaaagtcg gcttcgataa agttgaaatc gacccgatgg gcaacgttct
     3001 cggttatatc ggccacggcc cgcgtctggt ggcaatggac gctcatatcg ataccgtcgg
     3061 cattggcaac atcaaaaact gggacttcga tccgtacgaa ggcatggaaa ctgatgagct
     3121 aatcggtggt cgcggtactt ccgaccagga aggcggcatg gcatctatgg tttatgccgg
     3181 taaaatcatt aaagacctcg gtctggaaga tgaatatacc ctgctggtta ccggtactgt
     3241 gcaggaagaa gactgcgacg gtctgtgctg gcagtacatt attgaacaat ccggcattcg
     3301 cccggaattt gtggtcagta ccgaaccaac cgactgccag atataccgtg gtcagcgcgg
     3361 tcgtatggaa attcgtattg atgttcaggg tgttagctgc cacggttctg cgccagaacg
     3421 cggtgacaac gccattttca aaatgggtcc gattcttggc gaattacaag aactctccca
     3481 acgtctgggt tatgacgaat tcctcggcaa aggcaccctc accgtttctg aaatcttctt
     3541 cacatcccca agccgttgcg ctgtagcaga tagctgcgcc gtctctattg accgccgtct
     3601 gacctggggc gaaacctggg aaggcgcgct ggacgaaatc cgcgccctgc ctgcagtaca
     3661 gaaagctaac gcggttgttt ctatgtacaa ctacgaccgt ccgtcctgga ctggcctggt
     3721 ttacccaacc gaatgctact tcccgacctg gaaagtggaa gaagatcact tcaccgttaa
     3781 agcactggtg aatgcctacg aagggctgtt tggcaaagcg ccggttgttg ataagtggac
     3841 cttctcaact aacggcgtat ctatcatggg ccgtcacggc attccggtga tcggctttgg
     3901 cccgggtaaa gaacctgaag cgcatgcacc taacgaaaaa acctggaaat ctcacctggt
     3961 gacctgtgcc gcgatgtacg ctgcaatccc gttaagctgg ctggcaacag agtaattact
     4021 cttcacttat tcccctccgg ttcgccggag ggtttttgga gtttgctatg cgcgtattga
     4081 tcaaaaacgg cactgtcgtt aacgcagatg gacaagccaa acaggatttg ctgattgaaa
     4141 gcgggattgt tcgccagttg ggcaacaata tttcgccgca gctcccgtat gaagaaattg
     4201 atgccactgg ctgttacgtt ttccctggcg gcgtggatgt ccatacgcat ttcaatattg
     4261 atgtcggcat cgcgcgcagt tgtgatgatt ttttaccggt acccgcgcag ctgcgtgtgg
     4321 cggtacaaca accattattg accatatggg atttggccca aacggctgtc ggttacgcca
     4381 tcaactggag gtttatcgtg gttatgccgc ccataaagcg gtcatcgatt acagctttca
     4441 cggtgtgatc cagcacatta atcacgcaat cctcgacgaa atcccgatga tggtcgagga
     4501 aggactgagc agttttaaac tctatttaac ctatcaatac aaactcaacg atgacgaggt
     4561 tttgcaggca ttacgccgct tgcatgaatc cggcgcgctg accaccgtgc acccggaaaa
     4621 tgatgcggct atcgccagca agcgggcgga atttatcgcc gcagggttaa ccgcgccgcg
     4681 ctatcatgcc ttgagtcgcc ctctggaatg cgaagcggaa gccatcgccc gcatgattaa
     4741 cctggcacaa attgccggta acgccccgct ctatatcgtg cacctgtcta acggcttagg
     4801 tctggattat ctgcgtcttg cccgtgcgaa tcaccagcca gtctgggttg aaacctgccc
     4861 acaatatctc ctgttggacg aacgcagtta cgatacagaa gatggcatga agttcattct
     4921 tagcccaccg ctgcgtaacg tacgcgagca ggacaaactg tggtgtggca tcagcgatgg
     4981 tgcgattgac gtggtggcga ccgatcactg caccttctcg atggctcaac gcctgcaaat
     5041 ttctaaaggc gatttcagtc gctgcccaaa tggcttaccc ggtgtggaaa accgcatgca
     5101 gttactgttt tccagtggcg tgatgacggg acgtataaca ccggaacgct ttgttgaatt
     5161 aaccagcgca atgcccgcca ggctgtttgg cctgtggccg caaaaaggat tattagcgcc
     5221 cggttccgat ggcgacgtgg tgattatcga cccacgtcag agccaacaaa ttcagcatcg
     5281 ccatctccac gacaacgccg actactcgcc atgggagggt tttacctgtc agggcgcgat
     5341 tgtcagaacc ttatcccgtg gtgaaacgat tttctgtgac ggcaccttta caggcaaagc
     5401 cgggcgaggt cgtttcctgc gacgcaaacc gtttgtccct cccgtgctct aaccactgtc
     5461 agggatctat acacaatcaa gaatgatgag tgaggaaaaa tgagtaagaa aattgttctc
     5521 gccctgggcg ggaatgcgct gggcgatgac ctggccggac aaatgaaagc ggtaaaaatt
     5581 acttctcagg caattgttga tttaattgcc caaggacatg aagttatcgt cactcatggt
     5641 aacgggccgc aggtgggcat gattaaccaa gcctttgaag ctgccgcaaa aactgaagca
     5701 cactcgccaa tgctgccgat gtctgtctgt gttgccttaa gccagggtta tattggttac
     5761 gatctacaaa acgcgttaag agaagaactg ctttctcggg gcattaataa acctgtagca
     5821 acgctggtta ctcaggtcga agtcgacgct aacgatccgg cattcctcaa cccgaccaag
     5881 ccgatcggtt cgttctttac cgagcaagaa gcagagcaac tgacaaaaca gggttacact
     5941 ctgaaagaag atgctggtcg cggctatcgc cgtgttgttg cctcgccaaa accggttgat
     6001 atcattgaaa aagaaacggt taaagctctg gtagatgcag gccaggtggt gatcgccgtt
     6061 ggcggtggtg gtatccctgt tattcgtgaa ggtaaccatc tgcgtggtgc cagcgcggtt
     6121 atcgataaag actgggccag cgcccgttta gcagaaatga ttgatgccga tatgctgatc
     6181 attctgactg cggtagaaaa agtggccatt aacttcggta aagagaatga acagtggctc
     6241 gatcgcctgt cattaagtga tgctgaacgc tttatcgaag aaggtcattt tgcgaaaggt
     6301 tctatgctgc ctaaagtgga agctgccgcc tcgtttgttc gctctcgcgc aggtcgtgaa
     6361 gcactgatta ccgtattgag caaagcgaaa gaagggattg aaggcaaaac cggaacggtg
     6421 atttgtcagt aattttaagg ccgggtgggt aataccatcc ggccattttc atttaagcca
     6481 cttccagcac ttcttttgtc gctttcacgc cctgatgcat caacatcatt aacgcctcaa
     6541 gtacgccgcc gccaatcgcc cgggctttat cagaaacgct ggtgaaatca gcggtttcac
     6601 cacgaggatc gatatcacca attttaaaac caccaaccac tgccaggccg tcgttcaaca
     6661 agccacgcac catccccgtc aacggtgctt taatttcatg ctcaccaatc caggcaatca
     6721 catcgccctc tttcaccaga tcgcctaatt tcacgttgga tcgcataatg cctgcagcag
     6781 gagcacggat cacccgtcgg gtggtatgcc ccataatatt gccaggaaca tcggtattct
     6841 cctgcgcaca accagagtaa atcacctgac cgagccagtg cccgcgattt gtttcaatta
     6901 ccgcatgaca atccttccct gcagtaaagc ccggcccaag cgcgattgtt actggtgcca
     6961 tatctgcccg cgttcccaaa ttctgtttcg ccagaatagc gtccaccacg caaagcggtt
     7021 tcagttcatc aagcaatgaa caggcgggat ctaccatcac agggatgaat ccgcgttcgg
     7081 taagtttcat cgcttccgca gagctggttg ccaggcgagc agtgacgcct tcgaccgtca
     7141 tttcgccatc gaacacggcc tgggcaaacg ccacggtaca acgaatcact gtcggttttt
     7201 ccacttccag catgatcact ttaaaacccg catgatacag acgtagcgcc acaccactgg
     7261 cgatatctcc cgcaccgcga atcaccacca gctggtgtcc ggaaggatga gaaggtttca
     7321 tcatcagccc acccggcgcg ttatttttca cccataatat ttctgccagc acgctgatgg
     7381 cgatctcctg cggcgtttcc gcacctatgt tgtaaccaac gggcgcatgt aaacgggcaa
     7441 tatgttcttc agccacgcct ttctcacgca attgacgcag gaaaagctga accttgcggc
     7501 gacttgccag caaccccaac catgcaatgg gctgttcaat gagtttgtcg agggcttcac
     7561 gatcctgatt attcgtggca atcaggacaa aattatcagg gcgaatatcc agttcttcca
     7621 ctgccgcacc aaacgactca gcatggagaa gcgtggttga tggtgggaat agttcaggat
     7681 tgaggctttc gcgataaata tcggcaacgg caatatcaaa tcctaatagc gccgcactct
     7741 gggctatcgc ccggttgaca tgccccgcgc caatcaacac cagacgtgga cgcataccat
     7801 ggacgctgat aaatactgac attgcacctc cacaatctga cccgacagca tccgcaccgt
     7861 tacgagccat acgtccatgg aataatcgcg gcttacgttc ctgcaatgcc tgaagtgact
     7921 cttcaatcac cttccgctca accattccgc caccaattgt accgacgata gaaccatcgg
     7981 cgcgcactaa catttgtgca gaatggcggg gagtcgagcc tcggctatca acaatttgcg
     8041 ccatcgcaaa cggacaattt tgctcttcga gttttgcagc ctctgtgaaa atattcatac
     8101 caacctcaat ggatatcctt tcagtaaccc ggaatacccg ggccgctaat tcacaaatct
     8161 gcgcgcgatt gcaggatgtt cttgtatatc gcccagccag attgcctcta cgtcgtgttg
     8221 ttgcagcggt tgaaggagct cgctttgtgc aatcgcattc tcacattgag aaaaacggtt
     8281 aatgaaccag actcgccgac aaccttgcgg tacgttttta aacgccccct gagggtggcg
     8341 aaccagcgca acgagatcgc tcagttgcaa ggttgcatca ggtgttaacc cggtaatatc
     8401 agcaaactgc gaccagcgat ggacattttc tgtgctcact ttcgcgccca aagtatgtcc
     8461 tcccatcaca gcaatcacgc agcaactgct tttgggtatg caaggttcgt gctcatcagg
     8521 cgcttttaac ggcattccac gcgagccatc tgcttcaatg agaattacgt cacactctgg
     8581 tcgttgcacc agtgcatcaa tcgcttctgg cgtaaatccc tgcacttttc cctggttcgc
     8641 tttccagctg tgaaaacaaa atgaaatggg ggatgtaaga gacgcatgag gaagcatggc
     8701 gggatcacga cagaaaacca cgggccagtg agacgttggc ataaacatat gtgtcgtcgt
     8761 cgtgattaac acacgcctgc cgctagcctg gagcagttct gccagccaaa aaagcaagct
     8821 ggttttcccc ccagcaccaa caacagaaat cacagtggga cgtttctgcg cacctaaatc
     8881 aatgactaat gccgatgggt caactatact tttcacgagt ctttatgacc tctctgggat
     8941 aaattatccc caacttaatc catcaggaag taacgcaatt atcaggcgtt attagcccct
     9001 ataaataatg gaaccactat gtcagccatc gactgtataa ttaccgctgc cggattatca
     9061 tcaagaatgg ggcaatggaa aatgatgttc ccctgggaac agggaacaat tcttgataca
     9121 agtatcaaaa atgcgttgca gttttgtagc cgaattattt tagtcaccgg ctatcgtggt
     9181 aatgaactgc acgaacgtta tgcgaaccag agcaatatca ctattattca caacccagat
     9241 tatgcgcagg gtttactaac atccgtcaag gccgcagtac ccgcagtgca aacagaacat
     9301 tgttttctca cccacggtga catgccaacc ctcaccatcg atatttttag aaaaatctgg
     9361 tcgttacgaa atgatggcgc aatactgcca ctccataatg gcatccccgg ccatccgatt
     9421 ttagtgtcaa aaccatgcct gatgcaggca atccagcgac ccaatgtcac caatatgcgt
     9481 caggcactcc ttatgggaga tcattattcc gtcgaaatag aaaatgcaga aataatttta
     9541 gatattgata ctccggacga ctttattaca gcgcaaaaaa ggtatactaa aatttagaaa
     9601 atgtagttaa acgattgcgt tcaaatattt aatctctcca gcactcttta tcataatgat
     9661 aaggaatcac tgattttgag aaaagagaaa aagtgataaa tgggctgaca ataataattt
     9721 tcaggagtga ttttaattct catttggcac ctgtgagttc attcacaaaa agcctttaag
     9781 tttcgagcca cctcacattt ttttatattt cccgccaaac ctggcaagag tggtgcgatt
     9841 gttgctctat ccccctaaac caccggattt ctcaacaccg gtcactcaat gatatctgta
     9901 taagctaagg agagggttat gggggatatt atgcgtccca ttccgtttga ggaacttttg
     9961 acgcgcatat ttgatgaata ccaacaacaa cgctcaatct ttggtattcc cgagcaacag
    10021 ttttactcac ctgtaaaagg taaaactgtt agcgtcttcg gtgaaacctg tgccactccc
    10081 gtcggccctg ccgctggccc gcacacgcag ctcgcgcaaa atattgtcac ttcctggctg
    10141 actggcggac gcttcatcga actaaaaacc gtccaaattc ttgaccgcct ggagctggaa
    10201 aagccctgta ttgatgccga agacgaatgc tttaacaccg aatggtctac cgaatttacc
    10261 ttgcttaaag cctgggatga atacctcaaa gcctggtttg ccctgcacct tctcgaagcg
    10321 atgttacagc cttctgattc cggtaaatcg ttcatcttta atatgagcat cggttacaac
    10381 ctcgaaggta ttaagcaacc gccgatgcaa cagttcatcg acaatatgat ggacgcatct
    10441 gaccatccga aattcgctca atatcgcgat acgctgaata aattactcca ggatgacgca
    10501 tttttagccc gccacggatt gcaggaaaaa cgcgaaaact tgcaagcctt acccgctcgc
    10561 atccccacca gtatggtgca gggcgtcccc ctctccacca tgcacggctg tcctccgcat
    10621 gaaatcgaag ccatttgccg ctacatgctg gaagaaaaag ggctcaacac ctttgtcaaa
    10681 cttaacccga ccttactggg gtacgcgcgt gttcgtgaga tcctcgatgt ctgcggtttc
    10741 ggttacatag gcttaaaaga agagtcattt gatcacgacc tcaagctgac gcaagcactg
    10801 gaaatgctgg agcgcctgat ggtactggca aaagaaaaat cactcggctt tggcgtaaaa
    10861 ctgactaaca ctctcggcac catcaacaat aaaggcgcac tgcctggtga agagatgtat
    10921 atgtcaggcc gtgcgctgtt cccgctctcc atcaatgttg cagcagttct ctctcgcgcc
    10981 tttgacggca aactgcccat ttcttattcc ggtggtgcca gtcagctgac tatccgcgat
    11041 atttttgata caggtattcg ccctattact atggcaaccg acctgctgaa acctggcggc
    11101 tatctgcgct taagtgcctg catgcgcgag ctggaaggct ccgacgcctg gggacttgac
    11161 catgttgacg tcgaacgact gaacagactg gcagcagacg cgttaaccat ggaatacacc
    11221 cagaaacact ggaagccaga agagcgtatt gaagtggcag aagacctgcc gctgaccgat
    11281 tgctacgttg ccccctgtgt tactgcctgc gctatcaagc aagatattcc ggaatacatc
    11341 cgtctgcttg gcgaacaccg ctatgccgac gcgctggaac tcatctacca acgcaacgcc
    11401 ctacctgcca ttaccggtca tatttgcgat caccagtgcc aatacaactg tacccgcctg
    11461 gattacgaca gtgcgctgaa tatccgcgaa ctgaaaaaag tcgcgctgga aaaaggttgg
    11521 gatgaatata agcaacgctg gcacaaaccc gccggttctg gttcacgcca tccggttgcc
    11581 gtgattggtg caggtccggc gggtctggca gcaggttact tccttgccag agcgggccat
    11641 ccggttacgc tgtttgaacg cgaagccaat gctggcggcg tggtgaaaaa tatcattcct
    11701 cagtt
//
LOCUS       DAZOXR010000134        11565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZOXR010000134 DAZOXR010000000
VERSION     DAZOXR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.136"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            174..845
                     /locus_tag="ACHUNN_003111"
     CDS             174..845
                     /locus_tag="ACHUNN_003111"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR0577417.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            855..2051
                     /locus_tag="ACHUNN_003112"
                     /pseudo
     CDS             855..2051
                     /locus_tag="ACHUNN_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2051..2611
                     /locus_tag="ACHUNN_003113"
     CDS             2051..2611
                     /locus_tag="ACHUNN_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0577418.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2613..3386
                     /locus_tag="ACHUNN_003114"
     CDS             2613..3386
                     /locus_tag="ACHUNN_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR0577419.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3574..3849
                     /gene="frmR"
                     /locus_tag="ACHUNN_003115"
     CDS             3574..3849
                     /gene="frmR"
                     /locus_tag="ACHUNN_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR0577420.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3884..4993
                     /gene="frmA"
                     /locus_tag="ACHUNN_003116"
     CDS             3884..4993
                     /gene="frmA"
                     /locus_tag="ACHUNN_003116"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR0577421.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5086..5919
                     /gene="fghA"
                     /locus_tag="ACHUNN_003117"
     CDS             5086..5919
                     /gene="fghA"
                     /locus_tag="ACHUNN_003117"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0577422.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6145..6684)
                     /locus_tag="ACHUNN_003118"
     CDS             complement(6145..6684)
                     /locus_tag="ACHUNN_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR0577423.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6786..7997)
                     /gene="mhpT"
                     /locus_tag="ACHUNN_003119"
     CDS             complement(6786..7997)
                     /gene="mhpT"
                     /locus_tag="ACHUNN_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR0577424.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8284..9297)
                     /gene="mhpE"
                     /locus_tag="ACHUNN_003120"
     CDS             complement(8284..9297)
                     /gene="mhpE"
                     /locus_tag="ACHUNN_003120"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR0577425.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9294..10244)
                     /gene="mhpF"
                     /locus_tag="ACHUNN_003121"
     CDS             complement(9294..10244)
                     /gene="mhpF"
                     /locus_tag="ACHUNN_003121"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR0577426.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10241..11050)
                     /gene="mhpD"
                     /locus_tag="ACHUNN_003122"
     CDS             complement(10241..11050)
                     /gene="mhpD"
                     /locus_tag="ACHUNN_003122"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR0577427.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11060..>11565)
                     /gene="mhpC"
                     /locus_tag="ACHUNN_003123"
     CDS             complement(11060..>11565)
                     /gene="mhpC"
                     /locus_tag="ACHUNN_003123"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0577428.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2783 a         3119 c         2887 g         2776 t
ORIGIN      
        1 atatgaattg cataaaaaca ttacccagtc gaacctccgg gtctgaaagc ccttgctttt
       61 cagttattag caacatttaa catttataaa ttaacatctg gctgcgccag agattacgtc
      121 tctgagtgca tcgttgtgct tgtagtataa taaataaaat ggagtgtcat taagtggata
      181 aggttttaga ttcagccctc ctttcttcgg caaataaaag aaagggtatc cttgctattg
      241 gcgcacatcc tgacgatata gaattaggct gtggcgcatc gcttgctcgt cttgcgcaaa
      301 aaggaattta tatcgcagca gtggtgatga ctaccggcaa ctctggtaca gatggaataa
      361 tcgatcgcca tgaagaatcg cgcaacgccc taaagatatt agggtgcaac caaactattc
      421 atcttaattt tgctgacacc cgcgctcatt tacagctcaa tgatatgatt tccgccctgg
      481 aagacatcat taaaaatcaa attccttctg atgttgaaat catgcgggta tataccatgc
      541 atgatgccga ccgccatcag gatcatctcg ctgtttatca agcttcaatg gttgcctgcc
      601 gcactattcc acaaattctc ggctacgaaa ccccaagtac ctggctttca tttatgcctc
      661 aggtttttga atccgttaaa gaagaatatt tcacagtcaa acttgctgca ttaaaaaaac
      721 ataaaagcca ggaacgacgt gattatatgc gtcatgatcg cctgcgtgca gttgcacaat
      781 ttcgcgggca acaggtcaat agcgatctgg gtgaaggctt tgtaattcat aaaatgattc
      841 tttgagcaaa ttgcatgaaa acctggatat ttatctgtat gtccatagca atgttgctat
      901 ggtttttaag tacgctaaga cgtaaaccca gtcaaaagaa aggctgtatt gacgccatta
      961 tacctgcgta taacgaaggc ccgtgtctgg cgcagtcact ggataatcta ctgcgtaacc
     1021 cttatttttg ccgggtaatt tgcgttaacg acggctccac ggacaatacc gaagcggtca
     1081 tggcggaagt caaacgcaaa tggggcgacc gctttgttgc cgtcacgtaa aaaaataccg
     1141 gtaaaggtgg tgcgctgatg aatggcctca attatgccac ctgcgaccag gtttttttaa
     1201 gtgatgccga cacctatgtt ccgcccgatc aagacggaat gggctatatg ctggcagaaa
     1261 ttaagcgcgg tgctgatgcc gtaggcggca ttccctctac tgcgttgaaa ggcgcggggc
     1321 tgttaccgca catccgcgcg accgtaaagt tgccgatgat tgttatgaag cgcacgctac
     1381 agcagctcct gggcggcgca ccgtttatta tcagcggtgc ctgcggaatg ttccgtactg
     1441 atgtattgcg taagttcggt ttctcggatc gtactaaagt cgaagacctt gatctcacct
     1501 ggacattagt ggcaaacggc taccgtattc ggcaggcgaa tcgctgcatc gtatacccac
     1561 aggaatgcaa cagtccgcgt gaggagtggc gtcgctggcg gcgttggatt gtgggctacg
     1621 cggtctgtat gcgcctgcat aaaaggcttt tatttagccg cttcggtatc ttcagtatat
     1681 ttcctatgct gttggttgtg atttatggcg ttgggattta tctcactacc tggtttaatg
     1741 aattcatcac caccgggccg catggtgtgg tgttggcaat gtttccgctt atctggatcg
     1801 gcgtagtttg tgttattggt gcttttagcg cctggtttca tcgttgctgg ttgttggtgc
     1861 ctttagcgcc gctttccgtt gtgtatgtat tattagctta tgccatctgg attatctatg
     1921 gacttattgc cttttttact ggacgcgaac ctcagcgcga caaacccacc cgctattccg
     1981 cactggtgga agcgtcaacc gcttattccc aaccttctgt cacaggaact gaaaaactat
     2041 ctgaagctta atgttaaaga gaaaaattat tcagattgca gaccaggtaa ttattgatga
     2101 aactgcaggt gaagttgtta tcggcgcgaa tacccgtatt tgtcatggtg ccgttattca
     2161 gggtccggta gtgattggcg caaactgcct gataggtaat tatgcgttta ttcgtcctgg
     2221 cacaataatc agcaatggtg taaaaattgg ttttgccacc gaaattaaaa atgcggttat
     2281 tgaagcggaa gcaacgattg gtccgcaatg ttatattgcc gactcggtag ttgcaaacca
     2341 ggcatatttg ggcgcacaag tacgtaccag taatcatcgt ctggatgaac agcctgtgtc
     2401 tgttcgaact ccagagggaa ttatcgctac cggatgcgat aaattaggtt gttatatcgg
     2461 gcagcgttca cgccttggcg tacaagttat tattttgcct gggcgaatta tttccccgaa
     2521 cacgcaactt ggcccgcgcg tgattgtaga acgtaattta cctaccggaa cttattcact
     2581 ccgacaagaa cttatccgta caggagatta aaatgataaa acggacgtta ttagcggcgg
     2641 ccatttttag cgcattgccc gcttatgccg ggttaacttc cattaccgcg ggctacgatt
     2701 ttaccgatta ttctggcgat catggcaacc gtaatttagc gtatgctgaa ctggtggcga
     2761 aagttgaaaa cgcaacgctg ctttttaatc tttcccaggg gcgtcgtgat tatgaaactg
     2821 aacatttcaa tgccactcgc ggtcagggtg cggtctggta taaatggaat aactggctga
     2881 caacccgaac gggtattgcc tttgcggata atacgccggt ctttgcccgc caggattttc
     2941 gtcaggatat taacctggcc ctgttgccaa aaacgctttt cacgaccggt tatcgctaca
     3001 ctaaatatta cgatgatgtc gaagtcgatg cctggcaagg cggcgtatca ctctatactg
     3061 gcccggtgat caccagctac cgctataccc attatgactc cagcgatgca ggtggtagtt
     3121 atagcaatat gatttccgtg cgtctgaatg acccgcgcgg cactggttat acgcaactat
     3181 ggctaagccg cggaacaggc gcttacacct atgactggac gccagaaaca cgctacggca
     3241 gcatgaagag cgtcagtttg caacgtattc aaccgctgac tgagcaactt aatctcgggc
     3301 tgacggcagg taaagtgtgg tacgacaccc caaccgatga ttataacggt ctgcaacttg
     3361 cagcccatct gacctggaaa ttctgattgc ttctgccgcc cgctatccgg ggcggccttc
     3421 cctgccgatt agccccactc cctttcctcg ttgttttccg accacattca ccggataaat
     3481 tttattctcc agcgttatat actataggga ggtatgcatt gacatatagc atacctccct
     3541 atagtatatt gcgtgcagat gatgaggtgc gaaatgccca gtactccgga agagaagaaa
     3601 aaggtcctta ctcgagttcg tcgtattcgg gggcagattg atgctctgga acggtcgctg
     3661 gagggtgatg ccgaatgccg tgccatactc caacagatcg ctgccgttcg gggcgcggct
     3721 aacgggctga tggcagaagt gcttgaaagc catatccggg aaacgtttga ccgaaatgac
     3781 tgctacagcc gcgaagtcag ccaatccgtt gacgacacta ttgaactggt tcgagcctat
     3841 cttaaatagc tgaatctatt accatattga ggaagagcga gagatgaaat cacgtgctgc
     3901 cgttgcattt gctcccggta aaccgctgga aatcgttgaa attgacgttg caccaccgaa
     3961 aaaaggtgaa gtgctgatta aagtcaccca taccggcgtt tgccataccg acgcatttac
     4021 cctctccggg gatgacccgg aaggtgtatt cccggtggtt ctcggtcacg aaggggccgg
     4081 cgttgtggtt gaagtcggtg aaggcgtaac cagcgtcaaa cctggcgacc atgtgatccc
     4141 gctttacacc gcggagtgcg gcgagtgtga gttctgtcgt tctggcaaaa ctaacctctg
     4201 tgttgcggtt cgcgaaaccc agggtaaagg cttgatgcca gacggcacca cccgtttttc
     4261 ttacaacggg cagccgcttt atcactacat ggggtgctct acattcagtg aatacaccgt
     4321 ggtcgcggaa gtgtctctgg ccaaaattaa tccagaagca aaccatgaac acgtctgcct
     4381 gctgggctgt ggcgtgacca ccggtattgg cgcggtgcac aacacagcta aagtccagcc
     4441 aggtgattct gttgccgtgt ttggtcttgg cgcgattggt ctggcagtgg ttcagggcgc
     4501 gcgtcaggcg aaagcgggac ggattatcgc tatcgatacc aacccgaaga aattcgatct
     4561 ggcgcgtcgc ttcggtgcta ccgactgcat taacccgaat gactacgaca aaccgattaa
     4621 agatgtcctg ctggatatca acaaatgggg tatcgaccat acctttgaat gcatcggtaa
     4681 cgtcaacgtg atgcgtgcgg cgctggaaag tgcgcaccgc ggctggggtc agtcggtgat
     4741 catcggggtg gcaggttctg gtcaggaaat ctccacccgt ccattccagt tggtcactgg
     4801 tcgcgtatgg aaaggttccg cgtttggcgg cgtgaaaggt cgttcccagt taccgggtat
     4861 ggttgaagat gcgatgaaag gtgatatcga tctggaaccg tttgtcacgc ataccatgag
     4921 ccttgatgaa attaatgacg ccttcgacct gatgcatgaa ggcaaatcca ttcgaaccgt
     4981 aattcgttac tgatttcccg caggtatacc ccgtccactt cagacggggt tcttaatact
     5041 ctccccgggc agccgtccgg gggattaacc gtgagataat gactgatgga actcattgaa
     5101 aaacatgcca gctttggcgg ctggcaaaat gtgtatcggc attattccca atcactgaaa
     5161 tgtgaaatga atgtcggcgt ctatctccca ccaaaagccg cgaatgaaaa attgccggtg
     5221 ctgtactggc tttcaggcct gacctgcaac gagcagaatt tcattactaa atcggggatg
     5281 cagcgttacg cggctgagca caacattatt gttgttgcgc cggacaccag tccgcgaggc
     5341 agtcatgtcg cagatgccga ccgttacgat ctcgggcaag gtgccgggtt ttacctgaac
     5401 gcaacgcaag cgccgtggaa tgaacattac aaaatgtatg actatatccg caacgagctg
     5461 ccggatttag tgatgcaaca ttttccggca acgaccagaa agtctatctc tggtcattct
     5521 atgggcgggc tgggcgcgct ggtgctggcg ttacgtaacc cagatgaata tgtcagcgtc
     5581 tcggcgtttt cgcccattgt ctccccatcg caagtaccgt ggggacagca agcctttgcc
     5641 gcatatcttg gtgaaaataa agatgcctgg ttggattacg atccggtgag tcttatttca
     5701 caaggtcaac gcgttgcgga aatcatggtt gatcaggggt tgagtgatga tttttacgcg
     5761 gaacagctgc ggactccaaa tcttgaaaag atctgccagg agatgaatat caagacgtta
     5821 atccgttatc acgagggtta cgaccacagc tattattttg tctccagttt tattggcgag
     5881 catattgcct accacgccaa taaactgaat atgcgttgat aatagtgcac gactgccgga
     5941 tgcggcgtga acgccttatc cggcctacac ttcgcccgta aaccgtaggc ctgataagac
     6001 gcgcaaagcg tcgcatcagg catggcgcac gactgccgga tgcggcgtga acgccttatc
     6061 cggtctacac ttcgcccata aaccgtaggc ctgataagac gcgcatagcg tcgcatcagg
     6121 catctgcgca cgacttatct atgtttacca catcaaatca tcaggcactt tgaagtcggc
     6181 atacggatcg tcttcatcct gctcttcggc actgagcgcg ctgtgcaaca caatactgct
     6241 ggcatcacgc tgagcaattt tatcggcaac actggcggga ataatggtgt attcaccttc
     6301 gctattgtta tcgaccaaca agcgggcaat cgccagacga ccattaatca actgcgcctg
     6361 tgtgagctta tcgacaaaaa tcttcttaat cagattgccg tcagtgaagt taaaaccaat
     6421 atcgccattg gcaatggtga ttcggttcat ttcaatgagc tgcttcacct gggctttata
     6481 ttctttcgcc aacgccgctt gtttttgctg ttcgctaagc tgtttatcac gctcaagctg
     6541 tgccttttta ttttcttcta ccgcagcccg agcttcacgc gcctgaacgc gcgatttttt
     6601 cgccgttctc tcgaccttcg ccgctttttt gctggtgact aatcctgctt tgagcaactg
     6661 ctcttgtaag gtaagttttg ccatctttgt ttccaaaccc gctgaataat ttttagcgag
     6721 tatacctgta aacgccgtgg gtgtattggg ggcaagtccc tttcgcccct gcttaataag
     6781 gcgcgtcagg catcggcaca cggctgcatt cttgatctcc gactcatcaa aataaacacc
     6841 gccaacccag caacaagaat acccggtgca gaagcggcca ttacgccgac cgtgccagtg
     6901 cctaatgcca gcattttccc ggccagtaac ggaccgctca tagcccccag acgccctacc
     6961 gccacggctg ttcccacacc tgttgcgcgg atctgcgaac tgtaaaacaa cagtgccagg
     7021 gcatacaaaa cgctttgccc acctgtcgca aacaaccccg cgacaaatcc cgccagcaac
     7081 ataccgttaa atgacgacac cgttccaagc gccagcagcg aagctaacat gccgctataa
     7141 atcagtagcg acatggttac tggacgcagc ttatccatca atgcgcccaa cattaacgtc
     7201 ccgcttgccg cccccatttg cagggcaaac atcacccctg ccgcctgcga tggctggaat
     7261 ccttgctcca ccaaaagtag cggtagccag ttgatcaaca tataaaccac cagcagagtg
     7321 aagaaataac acaaccacag cagcagcgtc gcggttgccg tttctggcgc aaataaggca
     7381 cgcagtggtg gcgcagcctg tttttcgcca gcgaaaaccg ctgactccgg cagccagcgc
     7441 attaatagcg gcaccagaat caacggcacc acaccaccta cccaaaacac cgtttgccat
     7501 gctaagtttg cccccgcgaa acccagtgtc gccgccagcg ccgcgccaat gggaacaccg
     7561 caatacatca ggctcactgc cgtcccacga aaacgtggac ccgcggcttc agatgtcagg
     7621 gcgataagat tcggcaacgc cgcccccagc ccgacaccgg tcatcagccg cgcaaagacc
     7681 agtgagggga aatcccaggc aatcgccgtt gccagtgaga acaaaccaaa cagcgcaact
     7741 gagccaatca aaatacgctt acgaccataa cggtctgcca gcatcccgcc aaccaacgcg
     7801 ccgggtagca aaccgagtat tccggcgcta aatatccagc ccatttgcat tttatcgagt
     7861 gcgaaagcct gggcgatgcc acccgccgca atgccagccg cctgaagatc cagcccttcc
     7921 atcagagcga ccaaaaaaca aagcccgatg gtcagcatca ggcgggatga agatgatgaa
     7981 ggggtacgag tcgacataag atgaacctca ttgcgagtgc aggtgaatcg cgactgatac
     8041 attgccagcc gcgtaatcag aactatttaa tgactgccta cgggcgaaat gtagcgaaat
     8101 gtaggccgga taaggcgttc acgccgcatc cggcagtcgt gcgcagatgc ctgatgcgac
     8161 gctaacgcgt cttatcagac ctacaaattc tcacgccatc cgtaggccgg acaaggcgtt
     8221 cacgccgcat ccggctgtcg tgctgggatg ggcatacaaa atgcacgtta tcggcagtca
     8281 tcattatttg ttgttgcgca gatccagcgc cacgtcgacg atcatatcct cctggccgcc
     8341 aaccatccgg cgtttgccca gctcaacgag aatatccacc gcacttaagc cataacgcgc
     8401 cgccgccgtt tcacagtgac gcaggaagct cgagtaaaca ccagcgtatc ccagcgccag
     8461 cgtttcgcga tcgactcgta ccggacgatc ctgcaacgga cgcaccaggt cgtcggcggc
     8521 atccattaac gcatagagat cggtcccatg ctgccagccc agtttatccg cggcggcaat
     8581 aaacacttcc agcggtgcgt tacctgcgcc cgcgcccatt cccgcgaggc tggcgtcgat
     8641 tcggtcgcag ccctcttcca ccgccgcgat agagttcgcc acgccaagac tcaggttatg
     8701 gtgagcgtgc atgccggttt gcgtttctgg tttcaacact gctttcaggg cgcggaaacg
     8761 gtcacggata tcgctcatgt tcatcgcacc gccagaatcc accacataaa tacaggtcgc
     8821 accgtagcct tccatcagct ttgcctgctt ggcgagattc tccggcgtgg tcatatggct
     8881 catcatcaga aaaccaacgg tgtccattcc gagctcgcgg gcatactgaa tatgctgggc
     8941 ggaaacatca gcttcggtac agtgcgttgc cacacgaacc acccgcgcgc cagcctgcca
     9001 ggcatttttc agatcgtgaa tagtgccgat tcctggcagc aacaacgtcg cgattttggc
     9061 gtgcctcacc acatccgccg ccgcttcaat ccattcaagg tcgctatgtg cgccgaaacc
     9121 atagttaaag ctggaacctt gcaaaccgtc gccgtgggcc acttcaatcg aatccacgcg
     9181 ggcatcgtcc agtgctttgg caatctggcg aacgttttcc agcgaatact gatgacgaat
     9241 ggcgtgcata ccgtcacgca atgtgacgtc cgagatataa agttttttac cgttcatgcc
     9301 gcttctcctg ccttgcgcgc cagtgactgg gccatttttt ccgctgtcgc cagcgcactg
     9361 gaagtcataa tatcgaggtt gcccgcatag gcaggcagat aatgcgctgc gccttcgact
     9421 tccagccaga ccgctgtttt cagtccggag aattgcccca cgcccggtaa attgaccggt
     9481 ttatcctgcg ggataacttc aaactgcacg cgctgtttca ggcgataacc cggtacgtaa
     9541 gcctgcaccg cctcagccat ttcattgatt gaggcttcga tatcatcttg tgaagcttcg
     9601 tcgctcaata catacaccgt gtcacgcatc atcaacggtg gctctgctgg gttaagcaca
     9661 ataatcgcct tcccttttgc cgcgccgccc accacttcaa tggctcggga agtggtttcc
     9721 gtaaattcat cgatattggc acgcgtgcca ggtccggcag atttactggc gatagaagcg
     9781 ataatttcgg cgtaatgaac acgcgccacg cgtgaaactg ccgccaccat tggaatggtg
     9841 gcctggccgc cgcaggtgac catgttgacg ttcagttgat cgacgttcgc ctcgaggtta
     9901 accaccggca cgcagtaagg gccgatggca gcaggcgtca ggtcaattaa gcgaatatcc
     9961 ggtttcgctt cgcgtaaagc ggcatcgttt ttcacatgcg cgcccgcgct ggtcgcatca
    10021 aatacaatgt cgatatcagc aaattcaggc atgttcatca gcccgatcac cccttcatgg
    10081 gtggtggcga cgcccatacg tctggcacgc gccagaccgt cggactgagg atcaatgccg
    10141 accatcaccg ccatctccag atgctgaccg tgacgcaaaa ttttaatcat cagatcggtg
    10201 ccaatgttgc cagaaccgat aatggcgact ttacgcttac tcatgacaga cttccttttg
    10261 gggctgcgct tgaaaatgtc gcagcaactg aacctatgcc ttcaatatgg gcttcaaaac
    10321 gatcgcccgc attcaccgcc accatcgggc ctaatgcccc ggtaagaatg atatctccgg
    10381 cgcgcagcgg ttcacccaga ctggccattt tgcgtgccag ccagacggcg gcattaagcg
    10441 gatgtcccag gcattcgctg ccgcgcccgc tggaaacctc ttcgttatta cgcgtcatct
    10501 tcatggcgca gtttttcagg tctaaccccg ccggacgttg cgccggaccg ccgatgacat
    10561 acaccccgca ggaagcgtta tctgccacgg tatcgacaaa ctgaatcgac cagtcgcgaa
    10621 tgcggctccc caccacttcc agcgccggaa gtacccattc aatggcgtta tacagttcgt
    10681 cgaaggtgat atcagttgcg ggtaaatcgc ggttcaacac cagtgcgatc tccgcttcaa
    10741 tgcggggttg cagaacacgg gaaaaaggaa tgatttcgtt atcgccataa cacatgtcgg
    10801 caaataacgt cccaaaatcc ggttgatcaa cgcccagttg ttgttgcact ttcggatgcg
    10861 tcaggcccac tttacgccct accacgcgac gcccctgcac aacgtcatat tgcacattta
    10921 tgtgctgaat ggcgtaggcc gcttcagcgt gatcgatacc aatcagatcg cgcagcggcg
    10981 caatcgcttc gccctgctct gcggcgcggc gtaaatccgc cgccagttgc tcaagagtat
    11041 gcttcgtcat gaccattcct taagggcgtg cgaggaaatt cagcaccaat tgattgaaag
    11101 cgtcggcatg ttcccactgc gcccagtgac cacagtcgcg gaagatatgc agttcagaac
    11161 cggcaatgcc ggacagcaga cgcagacccg catccatcgg cacaaagcgg tcgttgcgcc
    11221 cccagacaat cagggtttgc gctttgattt ccgccagacg tgggccaaaa tccgggaact
    11281 gtttcggatt agcttccagg ctcttaacga agttttccag gtgatcgcgg cgcgacagca
    11341 tattattcag gcgcgcttca aacagggcgt cggtcaaatc gctggtatcg aaaacgaaga
    11401 tatccatcat cagcttcagg ttttcgatag tcggctgacg ataaagctga ttcagtcgct
    11461 taataccttc ggttggcatc ggcgtaaaca aactcatgcc gcccgtaccg ccgcccatca
    11521 gcaccagttt gccgacgcgc tccggccagt taagggtgaa cgcca
//
LOCUS       DAZOXR010000135        11561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZOXR010000135 DAZOXR010000000
VERSION     DAZOXR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.137"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            41..253
                     /locus_tag="ACHUNN_003124"
     CDS             41..253
                     /locus_tag="ACHUNN_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577429.1"
                     /translation="MKTTISSLITLEIGHVEKLADECVADILTELPNEQIQVGMHDAT
                     AFIFLLNNKRFTLINTGSGSLAVRTC"
     gene            352..2460
                     /locus_tag="ACHUNN_003125"
     CDS             352..2460
                     /locus_tag="ACHUNN_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFR0577430.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSRSLG
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSALDNHKSIESES
                     HSKHKHDNNDSDDSDNSESSETIFDDTENDKEVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASSYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVSSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            2556..3791
                     /locus_tag="ACHUNN_003126"
     CDS             2556..3791
                     /locus_tag="ACHUNN_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0577431.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKDAEINMLTNA
                     GIVDEYAEKMVKFAGIVRDGYEEHNISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            3972..7490
                     /gene="dnaE"
                     /locus_tag="ACHUNN_003127"
     CDS             3972..7490
                     /gene="dnaE"
                     /locus_tag="ACHUNN_003127"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0577432.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRDKLAKESKGCMESLERGRNYSFTALIK
                     NEQGYRDICELMTVANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITPGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDTISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTMKTVEEALEYGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNSKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKS"
     gene            7616..8047
                     /locus_tag="ACHUNN_003128"
     CDS             7616..8047
                     /locus_tag="ACHUNN_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577433.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDDARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            8165..9193
                     /locus_tag="ACHUNN_003129"
     CDS             8165..9193
                     /locus_tag="ACHUNN_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFR0577434.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVAAASATEDEGLDD
                     LLRELDSL"
     gene            9254..10198
                     /locus_tag="ACHUNN_003130"
     CDS             9254..10198
                     /locus_tag="ACHUNN_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFR0577435.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVASIAPQPIVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            10198..10464
                     /locus_tag="ACHUNN_003131"
     CDS             10198..10464
                     /locus_tag="ACHUNN_003131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577436.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            10467..11540
                     /locus_tag="ACHUNN_003132"
     CDS             10467..11540
                     /locus_tag="ACHUNN_003132"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0577437.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
BASE COUNT         3350 a         2647 c         2786 g         2778 t
ORIGIN      
        1 gtactcccta ccatatttta agttagaatg gattgattga atgaaaacaa ctatttccag
       61 cctaatcact cttgagatcg gacacgttga aaaattagct gatgaatgtg tagctgacat
      121 tctcaccgaa ttaccaaatg agcagattca ggttggtatg catgacgcaa cagcctttat
      181 attcctactt aataacaaac gcttcacgct tatcaatacc ggctctggct ctttagccgt
      241 cagaacctgt taacccctct gctacctgcg cgaatggctt agttccctgt tcgcgcagtg
      301 ctacattaaa cacactagta aataatttgt tttcataaca aaggattagc catgtctaaa
      361 aaacgttcca tcaaagaggt tcaggacttc cgtgacagtg taaaacgagt agtcgctctc
      421 ctttcaggta aaaacatccc tgttgcagaa cgaggagacg acgcttatgt acgctataac
      481 gatgatggag agccaattct cgtaaacatc ccatcaattc cggataacgc aacaccggca
      541 ttgatgaatg ctgtgcgcgg atttctcgat catgaggttg ctcacatttt gtttaccgat
      601 attcgtgtct ccaacaaaat gagagaaaaa ggacgcgttc cttcccggtc actagggaat
      661 gcattagaag acgtgttcat cgagcgaaaa atggggcagg tctttaacgg aacgagacgt
      721 aatctgatgg caactcagcg ccttataatc gaaaaagtct ttaaaccaaa ggcttcagag
      781 gctattgctt attgtggcaa agatcagcgc gcgctttttc taaacttctt tctctgtccg
      841 gttgtaagag cctgggatgg ccaagcaccg ttcgtagatt tcatggatga atactggcct
      901 gtcattgaga aaccaatttc attattaaaa gaacatggta tcgatgtggc cgtgcgtaac
      961 atgtcttgca ccgaggattg tgtaaaggtg gctgcgacca tagctaagat cctcaaagac
     1021 actgaaagtg aaagcaaagg taaggagtca gctccgggaa aaacttccga tccttcagac
     1081 gctgaccaga cggatgcctc tggagaaaac aatgaagaca acgaagatca tgagacaccc
     1141 tcagcgttag ataatcacaa atctatcgaa tcagaatcac acagtaagca caaacatgat
     1201 aataacgaca gtgatgattc agataattct gaatcatcag aaacaatatt cgatgataca
     1261 gaaaatgata aagaggtatc agattctgat gcttctgata acgcggcgtc agaatcatta
     1321 accgctgacc acgaaaaaag aaaaacgaca gaagacggct cttcagatat tccaactccg
     1381 tcaaaaatga gtctggaaga ggctttagag gagctggata gcatagaaga tgaagtcgga
     1441 ggcatgacag aagatgctct atccgaaacg attaaaagcg agttaacaga aagctcgaaa
     1501 agcgaataca ggccatacaa tcgctcatac gacttcatcg gctcgattga tcaggcagaa
     1561 gcccatatca aacggcttat taaaacattc tccgatattg atttaggagg atatccaatc
     1621 agccgctatc gcatcgttcc tgaaggcaac cagctcttcg acaaatatat tgaaaagcat
     1681 ctttcgtcag gtgtttcgtc gacgctggca aaagacctgg agcgagcaat agcaagcaga
     1741 aacagagttc agtttatacc gggccagcgt cgggggcgca ttcatggttc tagtatctac
     1801 agattagcaa tgaatgatga tcgcgtgttt cgtaaaaaag aagaatctaa agccgttaac
     1861 gcctgtgttc aacaagtgat tgatttatca ggttcaatga gtggtataac gatacaattg
     1921 gctcttgcaa gttcatatac catcgccgat gcccttgatc gaataaatgt tcccaacatt
     1981 atcaccggct tcactacatt tggtagtcat atggcggcag gagaacttaa ggctgtcaag
     2041 tatgagttct ctcgctttga atctttaatg ctacctatca tcaaaaattg gaatgaaaag
     2101 gtaagttctc gcgaagttcg ctcacgtatg gggtgcgtag gctacacatt cccacttctt
     2161 aataacgtgg atggtgaaag catagccagc cttgcatcgt tattttccgg tcgcatggag
     2221 gacaggaaga tcatgcttgt tctgagtgat ggcgcgccgt gggctgttgg gagaggtttt
     2281 gacgctcatt tgcgttcggt tgcgaagcaa attgaaacgc agactgacat tgatttgatg
     2341 gcaattggca tcatgactga cgcaccggag agattttact caaatcatgc cctggtaacg
     2401 agcgttgata gtcttggttc atctgtagtt actgaactat ctcgtatcat tttgaagtga
     2461 ttagaatagc cttaatgata agtaatcact tacgatattt gatggtatat ttatataaga
     2521 agttgaaagc tcattagaaa acaaaggaaa aacgcatgac tactactgca ctgcaaaatg
     2581 aaaaaaatcc ttctgattac cttgtttgca agtggtgcgg aaaatcattt cactatttta
     2641 agtcccatgt agccaatggt aattgcgagg gcattcctga gtcagtaaaa gatgccgatc
     2701 ctgacaccgt actgaaaatg tacacaacac agtttccaga tgagccaacg ctatcgaaaa
     2761 aggcacttga tgcaattcaa gctaaacgtg ccgagcaaaa aagcgaaatg gccaaatctt
     2821 ctggcttgac cagtagccct ggctacacag gcacagttga gtacaagaca gatctggtcg
     2881 cagctcacga actgctaaac gtaacggtgg aagaactcgg aacaaaacgt gggacgccgc
     2941 tcatggttag cgtcaacgtc aatacgccgt atccagagtt cgttcccgaa gtgaagaaag
     3001 gctacgtata tggcgacttc gaactgatca aagatatttt catgatgctt gaacttggca
     3061 tacctggcta tttgtggggt catgcaggaa caggcaaatc gtcattgcct acacagctat
     3121 gtgctttgct caatcgtccg ttgatccgtg cccaacatac agcatcaatg gaagaggcac
     3181 atgttacggg gcaaattctg gcgcgtgatg gctctacgta tttcgaacct ggcttgcttg
     3241 cgctcgcaat gaagcatggc tgggtttacc tcgcggatga atacgacttt gcgtttccac
     3301 aaattcttgg cgtgtatcag ccagtgctgg aaggtgaagc gttggtcatc aaagaggcaa
     3361 ctccagaatg gcgtcgcatt actccgcatg aacggtttgc tttcattggc actggcaaca
     3421 cgaacggatc tggtgatgaa accggcttgt accagggtac aaacatccag aacgccgcga
     3481 acttttcgcg ttttggcatc gtttcgaatg tgaaatacat gagcaaagac gcagagatca
     3541 acatgttgac aaatgcaggc atcgtggatg aatacgctga aaagatggtt aagtttgccg
     3601 gtatcgttcg cgatggatac gaagaacaca atatcagcca accgattggg cctcgtgagc
     3661 ttttgttgtc ggccaagatt ggaatgatgc gaggcgattt tgtgacaggt attgagcgtt
     3721 ctttcattaa caaactccct tcagcttctg cacaagcggc tcgtgaagtt gttcaaaaaa
     3781 tatttggttg atcgtgcgta aaggatgttt cggctctctt atcgctgctt ctgaaactgg
     3841 taaggcttgt ctggagtgtc cagacaagcc ttattgtcac caatcagcaa aagaagttgc
     3901 gatttcgatg catgggaagt tcgtaggctt cccaaatgac aaaatcaaaa aaaccagaaa
     3961 ggtaaaaaca catgaaggca ctgatggttc gaactgactt ctcacttggg gagtcggctc
     4021 taaaagcaga aaacgcggtg aagattgcca gagaagctgg ctacaccgct gtaatttcag
     4081 cagatagcat gaatattgcg agtgttattc cactacaacg tgccgctggt gacgacatgg
     4141 cggttatttg tggtgtgaaa ctaaacatcg ttgatgatcc cacatacgag caccgggata
     4201 aacttgctaa agaatctaag ggatgtatgg aatcattaga gcgaggacgt aactactcgt
     4261 ttaccgctct aattaaaaat gagcaaggat atcgcgacat ctgcgaacta atgacggtgg
     4321 ccaacacacg agaacagttc tactttgtac cgcgtctctc gctcgaacag ttggtttcta
     4381 catatgccaa aggcaacatc atcctgctca cttccgacat cggtagcgtg ttccaacgca
     4441 acgattttgc aaaaatcata agcacactga ttacaccggg tggaaaagac aacttctata
     4501 gtgtggttta tccgcaccct accccattct acgaccagat taacgtccgg gcgatgaaag
     4561 tcgccagcgc attgaaaata gagccagtgg cgttctatcc cgcttattac gaatcgatcg
     4621 acgatgcaga cattaaagac attgcgcaca tggttacgaa caacataaaa atcgaccagc
     4681 cgcatcgtct gcgtatcccc caccagcgag ataacgccgt caatggtcgc cgccatctcc
     4741 ttgaggcgct taaagccttc tccgttcgca tggatgtgcc ggtaacagct gcaatggcct
     4801 caacaacgca ggataccatt atcgatgcct gcacatggcg ctggcatgaa ttgccaccag
     4861 cactgcccaa gatggcagac gacgaacctg caacgctgat gaaactggct gttgcagggc
     4921 tgcgtaaacg tcttaccaca aaagagtttg gatacacacc acctgcttct gagaacaggg
     4981 tttatgttga gcggctaaag tacgaaatgg acacgctgac tcgcctggga ttctgtggtt
     5041 acttcctgat ggtgcgcgat ctgatgaatc atagtcgtga aactggcatt cctgtcgggc
     5101 ctggtcgtgg ttcctccgct ggatctctgg tggcatggtg cataggcata accaacgtcg
     5161 acccaatccg tcacggtctt ctgtttgaac gtttcatcaa ccctgagcgt ctcgacttgc
     5221 cggatgcgga tttggacttc agccaggcac gtcgccatga agtgatcgag tatctgaatg
     5281 aacgctacgg cgaagattac gttgcaggca ttccgaactt cacctacctg ggcgcagcct
     5341 ctgcactacg tgataccgct cgtatttatg gtgtggagtc cgcagatatg gcggtatcaa
     5401 aagaactgaa gaacgtcgag gatgatagcc ttccattgga agagctgcgc gaacaactgg
     5461 caagtctcga caaatacgca acaaaatatc ctgatgcatt caatgcagcc tgcaagttac
     5521 aaagccttat gcgtggcttt ggtagacatg cggcagggat gatcgtagca ggtgttcctc
     5581 tgacagaacg tacaccggtt gagcgccgtg gtgacgcgcg ttgtatcgca tttgacaagc
     5641 gttactgcga ggctatgggc ctaattaagc tggacgtgct tggcctggca actctcgatt
     5701 tgctcgatag tgcaaaacgc tacattaaag agaacacagg tgaagatatc aatcttgata
     5761 ccatttctct tgaagatcgc aaggtgctgg atggttttgc tgctgggtac actcaaggtg
     5821 ttttccagct tgaatcaggc ccaatgcgca agctgcttaa agatctgggc ggtggcattg
     5881 agccaatgag ctttaaaacc gttgtcgcca cgactgcgct cttccggccg ggaccaattc
     5941 agtcaggcat gttggatgat tatgtttctg tcgccaaagg ctttatgacg ccggaatcat
     6001 tacaccccgt tcttgatgaa cttaccgcag aaacaaatgg cgtgattctc tatcaggaac
     6061 agacgatgaa cgcgactcga ttgcttgctg gcttcacaat ggctgaagct gacggcgttc
     6121 gtaaagcgat aggcaaaaaa gacatggaaa aaatgaagag catgggcgag aagttcatcg
     6181 ttcaggctca agctggctgg atagacgttg agctggaaga tggcactaca cagcgcattc
     6241 accgtgcgga acattttaaa tgcgaagacg gaactatgaa aactgtcgaa gaggcacttg
     6301 agtacggcgc aaaactacct ataaacgcag tacgcgttac agcgtcacat ccagggctat
     6361 cagagatgaa agcgaaggag atctggaccg cattcgaaaa gaatggtgcc taccagttca
     6421 acaaatcaca ctctgttgct tactccttaa tcagttatca atctatgtgg ttgaaaactc
     6481 attatcccgc agagtttttc gctgctgctc tcactattct tggcgaagat aaacaccagg
     6541 ggctggttaa agatgcgctg acctatggta ttcgcgtatt gccaccagac gttaacgtgt
     6601 catctaaccg aattgagatc cgcacgcttg aagatggcag ccaggtgctg tatgcgccct
     6661 tctctgctgt gaaaggttgt tctgagaatg gctgccaggc catcatgaga gcgcgagaaa
     6721 aagttggcgg caaattcgag tcactggcgc aattcgaaga agctgtcgag aaacgtgcct
     6781 gtaatagtcg agtgcgcgaa tcactgcaaa aagtaggggc gtttgcatcc atcgagccag
     6841 gcagtctgcc agcaactgat ccagaacgtc tccgcgacca agcggaattg atgggaaatc
     6901 ttgtcataga cgcagttaaa gcctcacgtc cgtttgagat gaactccaaa cgttcggctg
     6961 aaattaacgt acttatgacg cggatggcgg ctgaaatggg cttaggtgat gaactaatcc
     7021 gccccagcat tggtattaag ccgaaaatca tgatcattct ggacaatgca aacggcaatg
     7081 atgctcgaac cggttacttc atggagaacg gatacgacga ttttaaggcc aagctgttga
     7141 cggctggaga tttgcgcatg ggcgatctct atgtcacagg cgtttgcaaa aaggtgaaag
     7201 acaaagaaaa ggactacacc aaagacgaga tcggccagtt caccgacttt atgcgtgaag
     7261 agatcaatct ggtacgtccg acctatgtgc tgacgtgtgg cagtcgggca acggcattgt
     7321 ttaacaacaa aaacaagcca tccgatttag tcggtaggaa ggagtatctt ccagaccttg
     7381 acgttacagt cttttacggg tttaacccca acattcttta cttcagacct gaagagggag
     7441 aacgactaga agcgatatta gccgacgtag cagaaactat taagtcgtga tccaataaac
     7501 atggcctcta tggccatgtt ttctcttatc ccctcatatc ccctatcctc gaactccttg
     7561 ccttatcatc acaatgatat aatcaatata agatgataag taaaaaggaa agcacatgat
     7621 caccgatatt tacgagaaaa taatgtctga tctcgagttt gaccgagaca atctggagga
     7681 agtctggcgt agacaacccc gccttttaat ggagtatggc tcaaaactcg ctcatgcaga
     7741 aagaagtgtc gcagaggcaa aacttaacct tgaagctgtt gaagcaaagc tatacgacac
     7801 agagcgtaag aacttgagta tgaacggcat taagttcaac gagtctgtac tggacgctaa
     7861 ggttaaaacg aacccacagt atctatctaa acgtcagaag ttggatgacg cacggcacat
     7921 agcagacata tacaaacatg ctgtcgccgc cttttcgcat cgccgagaca tgatcgttca
     7981 ggcgtcaaag atggccatcg ttgaattaga gcgattaggc tctgaacgct ttattactcc
     8041 ccgttgattt ttgatagata ataagtaagt actgatctat catttaacag ctcgaaagag
     8101 ccacgaatga acgaaagccc aacgcgcata gcgccatcgg ccaaatcaca acaaggagaa
     8161 acacatgtct aagacattac ttgatttgct taacaaaact cgtgaagaca ttgccgccaa
     8221 acgtggcaac aacgttgatc tgactcgctt aaaagacggc gtcaactata tccgcatctt
     8281 cccgaataaa gacgacccaa acggtaagtt cttccagact ttcggtatgc actacgttaa
     8341 gtatcagaac gaggaaggta aagaagcaac caacgcttat atttgtgagc aacatactca
     8401 cggtcgcgct tgtcagctat gcgaaatggt gatggaaggt cgcgctcgtc acaagggtaa
     8461 caaagcaatg gaagaacgca tcggtcaaat gcgtgccact cctcgctacc tggtcaacgg
     8521 cattctttct gctcgtgagg atttcgcaga tgctgagaaa tgccaattaa tcgagctgcc
     8581 gtctactgta ttcgatgata tctgcaaagc aatcaccgaa gacatcgctg atgatatcgg
     8641 caatccactg agcaaagagg aaggctacgc attcctgatt aaacgtactg gctctggtcg
     8701 cgataccaaa tatgacgtct cgcctaagcg taaagtctac aaaggcgata tcgaagataa
     8761 attctggaac acccagcatg atctgatcgc atacgcaaat caggctgatg aaactcgtct
     8821 tctgtcgaca gttcgcacta tgggtcgtct gattggcatc gctgcaccaa ctgccgcagc
     8881 atctgcacca gcaatttcct caaccgcgaa aacatcggct gcggcactac ctggatttgg
     8941 ctctgtcact ggtcatacag aaggagcgac ggctgtagca accgcgcaca caccggcttc
     9001 tgaaccaacc agtctggttg atgaagaaat cctccgtgcc gttgaaactg aatttaaacc
     9061 agaggcaagt tccgctgccg ttgccgtatc agtcaaagag tctgaagcag tcgcagcgac
     9121 atctgtagca gccgcatctg cgacggaaga tgaaggtctg gatgacctac tgagagagct
     9181 ggactctctg taatcccatt acgtgaccag taaggcgtct acggacgcct tactttttgg
     9241 aaggaatgta ccggtgaatt atctcttcgt agatggcaat agcctgggtt attaccacca
     9301 acaatctgac aaattgcaca acggcgaaat ggaagtacag gctgctttcg gctttgttaa
     9361 gaacgtccgt cgttatgcct ccatcctcca tgcccgacct atgattcttt gggatggatt
     9421 tagtgacaag cgtcgcgact tttacccgga ctacaaagca aatcgcgacg acgatcctga
     9481 tatgaaaaag atgaaggaag gctttgctat ccagaagcca tacatcctca aaatgatgac
     9541 cgcgcttgga gttacccaac tcattgcaaa agatgcagaa gcggatgatc tggccgggct
     9601 gctggtagcc agcattgcac cgcagccaat tgttgaacac atctatctgt taacaggcga
     9661 tagcgactgg cttcagttag ttcgcgaaaa cgtaagctgg gtaagcctgc gcgaagacgc
     9721 caaaaacaag caggttaatt ttgagcaatt tgcggagctg acaggattcg ctactcctcg
     9781 cgcatttttg gaagcaaaag cattacaagg cgataaatcg gacaacatta gcggtgttgg
     9841 tggcattggt gctggcggtg cgaaagagct gctgcatgaa tggggaagtg tcgcaacgat
     9901 ggtacgcggc atcaacgacg gctcaatcgt ggttgacaaa ggacgccata agaccgcctt
     9961 caacaaacta gcgaagaatg ccttcaacga gaaaacaggc tgtcgaatgc tcgaagcgtt
    10021 caagagaaac atcacgctaa tgaacctgat tgagacgaag tttccgccta ccgaaatcga
    10081 aacaatcaaa ggcaatcgtg acgtgaaagc attcgagcaa ctgtgctacg agctgaattt
    10141 ccgttcgttc cttgaagacc ttgaagtgtt tgttcttcca ttcgaaaggt attgctaatg
    10201 cttaaatcga ttattaatgg cgctacaacc acccctaccc aactggcaaa agagattgtc
    10261 ttttatcacg gtgagtacgc tgtcatcgca ctgccgtcaa ttctaggcgc tgccggaatg
    10321 aaagcgacag atcgcgagtt tggattagtc agcgagcagg tcgtaaaaat cctcgctcgt
    10381 gtatccagac tccttaacca cgatgcgatt gtattcgacg aatccgccgc tttaaaacga
    10441 atcaacgaaa caaaaggagc ctgatcatgg caaaaggaaa atccgcactg gcacttgctc
    10501 tgaaaaagaa aatcggtagc aacgacgaaa ttcagaaagt aactcattgg attgacacag
    10561 gctttcctcc gttaaacaaa gcgatttccg gtcgttacga tggcggtttc ccatgtggtc
    10621 gtatcgtaga agtattcggg ccaccaagcg cggggaaaac ctttttggca acggcagcaa
    10681 tgatctccgc acagaaacaa gatggtctgg cggtattcct cgatcatgag aacagttttg
    10741 acgttggcct ggctgtagcc aatggtttga atgcagatga ggatgacggt cagtgggtat
    10801 acaagcagcc tgataccttc gaagactctg tagagttgat cggcacaatt cttaaattgg
    10861 tacgtgatga agagcttatt cctgaatcag cacctatctg tatcgtggct gactcacttg
    10921 cgtctatggt tccgaactcc aaagccgaga agttcgaaaa gatggctgaa ggcactgcca
    10981 aagacaaaga tcagctaaac atgaacgaca atacggcact ggctcgtgcg acaagtgcga
    11041 acttccctac tctggctttg tgggcacgca aatacaacgc ctgcatcatc ttcttgaatc
    11101 aggttcgcac aaaaatcggt gtaatgtttg gcgaccctac tacgtctcca ggtggagatt
    11161 caccgaagtt ctacgcttct gtacgtatcc gtcttggtgc atcggtgatg aaggatggta
    11221 aagagaagat cggccaggac gtaggcgcag aatgcatcaa aaacaaagtt gcaccaccgt
    11281 atggcaaatg cacctggaaa ttctacttcg atcctactcg tggcctcgac gttatcgaat
    11341 cgctcgtcga gtacatgctg gaagaaggat acctgccaaa gaacgccagc gggcgagttg
    11401 aaattggtga caagaaatac accaaatcgc agatcgtcga gatgtatcgg gagaagccac
    11461 tggctgaaat cattgcggct ttgcaggcaa tcgacgaccg aagagcaaaa gacaacccca
    11521 ccgagtcagt agaagagtaa acacaaggcg tccacaggac g
//
LOCUS       DAZOXR010000136        11544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZOXR010000136 DAZOXR010000000
VERSION     DAZOXR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.138"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHUNN_003133"
     CDS             <1..279
                     /locus_tag="ACHUNN_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0577438.1"
                     /translation="NNVKVAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAY
                     INDKSIEILLLSCFAASDQKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            490..813
                     /locus_tag="ACHUNN_003134"
     CDS             490..813
                     /locus_tag="ACHUNN_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0577439.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            810..1640
                     /gene="amiD"
                     /locus_tag="ACHUNN_003135"
     CDS             810..1640
                     /gene="amiD"
                     /locus_tag="ACHUNN_003135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0577440.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1637..2650)
                     /locus_tag="ACHUNN_003136"
     CDS             complement(1637..2650)
                     /locus_tag="ACHUNN_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0577441.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2749..4179)
                     /locus_tag="ACHUNN_003137"
     CDS             complement(2749..4179)
                     /locus_tag="ACHUNN_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0577442.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4190..5191)
                     /gene="ltaE"
                     /locus_tag="ACHUNN_003138"
     CDS             complement(4190..5191)
                     /gene="ltaE"
                     /locus_tag="ACHUNN_003138"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0577443.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5228..6946)
                     /gene="poxB"
                     /locus_tag="ACHUNN_003139"
     CDS             complement(5228..6946)
                     /gene="poxB"
                     /locus_tag="ACHUNN_003139"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0577444.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7079..8047)
                     /gene="hcr"
                     /locus_tag="ACHUNN_003140"
     CDS             complement(7079..8047)
                     /gene="hcr"
                     /locus_tag="ACHUNN_003140"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0577445.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8059..9711)
                     /gene="hcp"
                     /locus_tag="ACHUNN_003141"
                     /gene_synonym="priS"
     CDS             complement(8059..9711)
                     /gene="hcp"
                     /locus_tag="ACHUNN_003141"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0577446.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9855..10754)
                     /gene="lysO"
                     /locus_tag="ACHUNN_003142"
     CDS             complement(9855..10754)
                     /gene="lysO"
                     /locus_tag="ACHUNN_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0577447.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11249..>11544)
                     /locus_tag="ACHUNN_003143"
     CDS             complement(11249..>11544)
                     /locus_tag="ACHUNN_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR0577448.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         2808 a         3062 c         3063 g         2611 t
ORIGIN      
        1 aataacgtga aggttgctgc ggatgctttc cttgaaatta ttttctctct taagaataaa
       61 tattgcatta aactattaag cgctcaggaa cgggcgttta tttacgaatt tgcaagaatg
      121 atcttcgctt atattaatga caaaagcatt gagatcttat tactttcttg ttttgcggcg
      181 tctgaccaaa aagcaatcca gaggtatata cctcagtcac aagatgggga agactttagg
      241 agtcatttac aatataagtt gcctctttcc gctcattaat gttgttggtg caacataata
      301 agacaggtaa gcggtaccct ataatgaaac caatatcggg ttatgcaacg gataatcgta
      361 aagacttaag tgggtgtttt tgacacaatg agatgatttc ctaaactgat caaaaccttc
      421 ttcccaacat ggcatatcag ttcgataatg ctttagagat cgaaggctta acaaacaagg
      481 agatcgatca tgcaattttc tacaacccca actctggaag gccagaccat cgttgaatat
      541 tgcggtgtgg tgaccggcga agcgatttta ggtgccaata ttttccgtga tttctttgcc
      601 ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg aaaaagaact gcgtaaagca
      661 cgggagatcg cctttgagga attaggctcc caggcgcggg cgctgggggc cgatgccgtc
      721 gtcggtattg atatcgacta cgaaacggtc gggcaaaacg gcagtatgct gatggttagc
      781 gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt cttctggctg gtcgctgccg
      841 ctctgttatt ggcagggtgt gcaggcgaaa aaggcattgt cgagaaagag ggatatcagc
      901 ttgatacccg acgccaggcg caggcggcgt atccgcgcat taaagtgctg gtgatccact
      961 acaccgcaga tgattttgat agctcgctgg cgacactgac cgataagcag gtcagctcgc
     1021 attatctggt ccctgcggta ccaccgcgat acaacggtaa accgcgcatc tggcaactgg
     1081 tgccggaaca agaactggcc tggcatgcgg ggattagcgc ctggcgcggg gcaacgcgcc
     1141 ttaacgacac ctctattggc attgagctgg aaaaccgtgg ctggcaaaaa tcggccggag
     1201 tgaaatattt tgccccgttt gaaccgacac agattcaggc gcttattcca ctggcgaaag
     1261 atattattgc ccgttatcac atcaagccgg aaaacgtagt ggcacatgcg gatatcgcac
     1321 cgcagcgcaa agacgatccg gggccattat ttccctggca gcaactggcg cagcagggga
     1381 ttggtgcctg gccggatgcg cagcgggtta acttttacct tgccgggcgc gcgccgcaca
     1441 caccggtaga taccgcgtca ttgctggagc ttttggcgcg ctacggttat gacgttaaac
     1501 ctgatatgac accgcgcgag cagcggcgcg tgattatggc attccagatg catttccgcc
     1561 cgacgttata taacggcgaa gcggatgcag aaactcaggc gattgccgaa gcattgctgg
     1621 agaaatacgg gcaggattag cgcggcagtt ttccgtggtc gcgtagccag gcggcagttt
     1681 tctcgatacc ttcatctagg gtgatgatcg gctgataacc taactcttcc tgcgcacgcg
     1741 taatatccag cgtaaagtca aaattcaact tggagacgcc gtagtgggtc agcggcggct
     1801 cttttgctga cttgcggcct aaacgctcca tgctgcgggc gatcatatcc agcatcgggt
     1861 aggggacgga acgaatacga cagtcaatat tcaactcgtc gatcagcttc tgcacgatgc
     1921 tgcgcagtgt gcgatgctcg ccgttggtga tattgtacac acgcccggaa ggtagcttat
     1981 cgcaggcttc ctggcttgcc agccacattg cgtgcacggt attttcatag taggtcatat
     2041 cgaccagcgc actgccgcca tgcggtaaca gaatactgcc gtagtggtgc atcatatgcg
     2101 ccagacgggg aataaagact ttatcgtgcg gtccgaacag actttgtggg cgcagaatag
     2161 taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat cacttcttcg ctggctgctt
     2221 tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa atcttcttta atatcgcgat
     2281 ggtggtgata atcgaagtac agggaggggg aagagatatg aataaagtta cgcacacccc
     2341 aggcgacagc ccattcaccc aggcggcgag tggcgcgaac gttagccaga tcgaaagcct
     2401 gttgtgtccc ccagggtgag gtaaagctgg agcagtgcca cagcgtatca atgcccgcga
     2461 gcatcacttt agcttgtgat gaaaccagct cggtcagatc cgccggaaca aactctgcgc
     2521 ccattttttc cagcaatttg cccattgcct cgttgcgacc ggtcgctcgc acgctgatgc
     2581 ctttctggca taaaaactct accgcgtttc gacctaagcc gctggtggcg ccggtaacca
     2641 gtaccttcat atcaatccac tgttgttgag aaaatgacgt gcgcattctt ccgtgatttc
     2701 ccccatgatg caatgggaaa catgaaagaa taacgcaggt tttgtcgatt aatctgtgct
     2761 ttgttctgcc agtctggcga tttgttttgc cattccgcga aaaataaaca gatgcgcggg
     2821 gatcatcaat aaccagtaaa acagccccgg cataccgtgc ggatgccaga aagcgcggac
     2881 atcgatagta cgatagtcgc ctttatcttc caggctaaaa cacagtcgtc ccagccccga
     2941 cgctttcatg ccaaataaca acgtaagttg tttttccggt tcaacgacaa tcactttcca
     3001 gctatccacc gcatcgccag tctgtaaata ttcgcgctcc gggcggcctt tcgccagctt
     3061 atgaccgatc gcgcggtcca tcaacgcccg tgtctgccac aaaatattgc caaagaaata
     3121 acgctcttta ccgccgattt ggttcactac ctgccataaa gcagcaaggc tggcggacgt
     3181 tttaacggta aaccccgcct gtttggcaaa ataaccgtac tccggtcgcc agcgggcaaa
     3241 ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt ttttcctcct ctttcaacgt
     3301 gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg atgagtgcac gtagcgcggt
     3361 atcatccgcc agcagatcgt gtttcagccc ctgaatcaac gccctggcgg tggtgggcgg
     3421 tacggaagta atcacattga gaaaccacac cgaaatccag cgggtgggga gggggatggg
     3481 gatcaaccag cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt gctgataact
     3541 gagcacctct ggtccggcgg cttcgaagat gcggtgttcg ctggccggat gatctaacaa
     3601 cgccaccaga tagtgcagca agttttccag cgcgatgggc gtggtgcgtg aacgtaccca
     3661 gcgtggcggc gttaacactg gcaggttgta gaccatatcg cgcatgactt cgaacgccgc
     3721 tgaacctgcg ccaacgataa ttccggcccg aagttcggtc acaggtacat tcgcttcacg
     3781 aagaatgtcc gccgtagcct gacgagcacg cagatgatcc gactgctcat gtggcggggc
     3841 ctgcaacgaa ctgagaaaga ttaattgctt aactggtact tcacgtagcg catcgcggac
     3901 gttgagagcc acctggcgct cctgagcgat aaaatcgccg ccttcgccca tgctgtgcac
     3961 cagaaaatag acggtatcga tatcctgcaa cagggccgga aggttatccg gccagctgag
     4021 atcgacttta tggcaactga cgtttgccag ttgcagcttt gcaagcctgt cgacatgacg
     4081 tgccgccgcc aggatctgat gcccttgctg gctgagtgtg cgcaccagat gctgaccaat
     4141 gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg tctctctcct taacgcgcca
     4201 ggaatgcgcg ccagtgggcg gcgacttccg ccagttgttc gcgcgagacg tcaagatgcg
     4261 tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt tctcgctttc atgtattcgc
     4321 ctaacgcggc agcattttct tccccgacgc gaacaaacag catattagtg tcctgacgca
     4381 tcacatccgc gcctgcttca cgcagttgct ccgccatcca ggcggcgttg tcgtgatctt
     4441 cctgcaaccg tgcgacgtta tttttcaggg catacatccc ggcggcagcc agaatcccgg
     4501 actgacgcat cccgccaccg gccattttcc gccagcggat ggcacgttta atgtaatcac
     4561 gattaccgac gagtaatgaa ccgactggcg tcccaagacc tttcgacagg caaatggtga
     4621 acgaatcaca atattgcgtg atctctttca gttcgcagcc gtaagccacc acggcattaa
     4681 agatgcgcgc accgtcaaca tgcagcgcca gattgcgctt gcgggtaaat tcccatgctt
     4741 ctttcaggta ttcacgcggc agcactttgc cgttgtgggt gttttctaga ctgagtaatt
     4801 tggtgcgggc gaaatggata tcgtcgggtt tgattttcat cgccacttta tccagcggta
     4861 gcgtgccgtc ggcagccgcg tcgatgggtt gcggttgaat actgcccagc accgctgcgc
     4921 caccggcttc aaacagataa ttatgcgcgg cctgaccgac aatatactct tcgccgcgtt
     4981 cgcagtgact gagcagagcg accaggttag cctgggtgcc ggtcggcaga aaaatggcgg
     5041 cttctttacc ggaaagctct gcggcgtagt cctgcagagc attaacggta gggtcgtctc
     5101 cgtaaacgtc gtccccaacc ggggcggcca tcattgcttc gagcatggcg cggctcggtc
     5161 gggtaacggt atcactgcgt aaatcaatca tggcatgtcc ttattatgac gggaaatgcc
     5221 acccttttta ccttagccag ttcgttttcg ccagttcgat cacttcatcg ccgcgtccgc
     5281 tgatgattgc gcgcaacata tacaagctaa atcctttggc ctgttcgagt ttgatctgcg
     5341 gtggaatggc taactcttct ttggcgacca ccacatccac cagcaccgga ccgtcgatgg
     5401 agaaggcgcg ttgcagggct tcatcaactt cagacgcttt ttctacacgg atacccgtaa
     5461 tgccgcacgc ttcggcaatg cgggcaaagt ttgtgtcgtg tagttcggtg ccgtcagtca
     5521 aatagccacc agctttcatc tccatcgcca caaagcccag cacgctgttg ttaaagacga
     5581 caattttcac tggcagtttc atctgcacta ctgagaggaa atcgcccatc aacatgctaa
     5641 aaccgccatc gccgcacatg gcgaccacct gacgttctgg ctctgtcgcc tgcgcaccca
     5701 gcgcctgcgg catggcgtta gccatcgaac cgtggttaaa cgaacctaac aggcgacgct
     5761 tgccgttcat ttttagataa cgtgccgccc acaccgttgg cgtaccaacg tcacaggtga
     5821 aaatagcgtc atcggcggca aaatgactaa tttgctgcgc cagatattgc gggtgaatgg
     5881 ctttctcgct cggtttagct aaatcgtcca gccctttgcg ggcgtcgcgg taatcttcca
     5941 gcgctttatc cagaaacttg cgatcggctt tttcttccac caatggaagc aatgcacgca
     6001 gagtcgactt gatatcgccg accagtgcca tatccacctt gctgtgagcg ccgatgctgg
     6061 ctgggttgat atcaatctga atgattttgg catcggtcgg gtagaaggcg cggtagggaa
     6121 attgcgtgcc gagtagcact aacgtgtcgg cgttcatcat ggtatggaaa cctgacgaga
     6181 agccgattaa cccggtcatt ccaacatcat acggattatc gtattcgaca tgttctttac
     6241 cgcgcagggc atgaacaata ggcgctttaa ttttcccggc aaactcaact aactctttat
     6301 gcgcccccgc gcagccgctg ccacacatca gggcgatatt gctggaataa cgcagcagtt
     6361 gcgccagttt gcgtaactct tcttcttccg gcgtcacgac tggttgtggc gcatgatacc
     6421 agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac gtcgcctggt aacacgacaa
     6481 ccgaaacgcc acggttaagc accgctttgc gcatggcaat cgccagtact tgtgggatct
     6541 gctccgggct ggaaaccagc tcgcaatagt gactacattc gcggaatagc tcttgtgggt
     6601 gggtttcctg gaaatagccg ctgccaattt cgctggaggg aatatgagcg gcaatcgcca
     6661 gtaccggaac gtgattgcgg tggcaatcga acaggccgtt gattaagtgc aggttgccgg
     6721 ggccgcacga tccggcgcag accgccagtt ctccgctaag ttgtgcttca gcgccagcgg
     6781 caaaggccgc cacttcttcg tggcgggtgg acatccactc gatggtgccc atgcgattaa
     6841 gactgtcact aagaccgttc agagagtcgc ctgtgactcc ccagatgcgt ttcacccctg
     6901 ccgattcgag tgttttggcg atataagctg caaccgtttg tttcatggtt ctccatctcc
     6961 tgaatgtgat aacggtaaca agtttagttc atctgacgga gggggaaggg atgggagaga
     7021 aaggaggcac taacggttaa atagcccgat gaaaggaata tcatcgggca taaggcgatt
     7081 atgcgagaac caaatccccc tgcggatggc aggagcaggc cagtacgtaa ccttcagcga
     7141 tttcggcgtc ggtcagcgtc attgtactgc tcaccgtata ttcaccggaa accacttttg
     7201 tcttacagca gccgcaaaca cccgcacggc aggcagcgac aaccggaacg ttattgcttt
     7261 ccagcgcctc cagtagcgtg gtgccaaccg gggcgtaaaa ttctcgtgcc ggttgcagtt
     7321 tggtgaattt cagaccgctg gtcgccgctt ctgctactgg agtgaagaat ttctctttaa
     7381 agaaacgcgt cacgccgagc gctttcactt cctgctctac ccaatccata tacggagccg
     7441 ggccgcaggt catcacggta cgtgaagcta agtcaggtac acctgccagc agttcgcgag
     7501 tgagacgacc agcgataaag ccttcggtaa cgttattttc tgccaccagc gttaccggat
     7561 agttacgcca ctcatcggcg aaaataacat cctgcggcgt acgcacgttg tagatcaccc
     7621 gcacatcggc ctgtggacgg ttcttcgcaa gccagcgacg catcgacata atcggcgtga
     7681 cgccgcagcc tgccgccagc aacaggaatt tatcttctgc tttatcgtcg caggtaaatt
     7741 cccccatcgc gtccgaaagc cagagataat caccgcgttt tacatcgcgc gtcagccact
     7801 gggagccgac accgtcatca atccgccgca cggtcagggt gatatattca ctcacgcctg
     7861 gcgtggagga aatggtgtaa gcacgcagcg tttccgctga gtttcgcacg ctgaccagtg
     7921 catattgccc ggcgcgatat gggtagtaat cgtggcaaat cagggaaatc gtccacacat
     7981 ccggcgtttc ttgcgtaatg tgatgaacct gcatccgcca cgggcattga ttcgttggca
     8041 tcgtcatcga caaactcctt acgcgctcaa cagttgcttc atgtcttctt caacagtggt
     8101 gatagaacgc aggccgaatt tctcgttcag caccgccagc aggtctggtg tcaggaaacc
     8161 aggtgcagtc gggccggtga cgatattttt cacgcccaga gaaagcagcg tcagcagaat
     8221 gacgatcgct ttctgttcaa accaggagag caccagcgac agcggcagat cgttcacacc
     8281 acagcccagt ttctctgcca gagtgacagc cagaataatc gctgagtaag catcgttaca
     8341 ttgacctgca tctaccagac gcggcagacc ttcgatatcg ccaaactcaa gtttgttaaa
     8401 gcgatattta ccacaggcga gggtcaggat caggcagtca tccggcacgc tggtggcgaa
     8461 atcggtgaag tagtggcgct cgccgcgtgc gccgtcacag ccaccaagca ggaagatatg
     8521 acgcagtttt tcacggctca ccagatcaat cagcgtatca gcagcgccaa gcagcgtctg
     8581 gcgaccaaaa cccacggtga taaggtgcgg aatttcgctg tacgggaagc ctgccatctg
     8641 ttgcgcctgg gtgataaccg cagagaaatc atcaccgtcc agatgacgca cgccaggcca
     8701 gccaacaatg ctgcgggtcc agatacgatc gtcataagcg cctacggttg ggtcgatgat
     8761 gcagttcgag gtcatcacga tggggccagg gaaacgagcg aactccactt gctgattctg
     8821 ccagccgctg ccgtagttac cgaccagatg cttgaattta cgcagctccg ggtagccatg
     8881 cgcaggcagc atttcgccgt gggtgtagac attaacgccc gtgccttcgg tctgttccag
     8941 caggttgtag agatctttga gatcgtgacc ggaaatcaga atgcatttac ccgccgtcgc
     9001 tttgacgttg acctgggttg gcgtcgggtg accgtattta ccggtttcgc ctgcatccag
     9061 aatgctcatc actttgaagt tcatctggcc gatttccatt gaacactcaa gaagcgcgtt
     9121 catatcggca ggccaggtcc ccagccacgc catgatttta tggtactggg cataaatatc
     9181 gttgtcgtat tgaccgagaa catgcgcgtg ttccatatag gccgccgcac ctttcaggcc
     9241 atacaggcac agcagacgca ggccgagaat gttttcgcca atcgccgctt tatctttgtt
     9301 aggggtaaat tctgctgcct gacgttgcag ctcgccgaga tcatcgctca ccagttgcag
     9361 gtcagccatc gggttatcga cgcgcgcgtt ggcatctaca gccaggcatt gtgctttcag
     9421 cgcctcgcgc aaggcaatcg cttcacgagc gtagccgaca atacgcggag aatcgaagtt
     9481 aacgttggtc agggttgaga aaaaggcacg tggcgcgaag ctgtcaacat cgtggttgat
     9541 gatgccgtat tcacacgctt ttaccgccca ggcagaaagc ccttgcagcg ccgcgatgag
     9601 taaatcctga aggtcagaag tttccgccgt tttaccacac atcccctgcg cgtatgagca
     9661 gccgtttcct gccggagtac ggatagtttg ttcacattgc acacaaaaca tgatcacacc
     9721 ttttaaagtt atatttaata tacatgttta aggttaagac gcttaacgcg gggataaaag
     9781 ggatttttca tgcaacttta agggagattg atttagcgca attttggcgg cagggatcta
     9841 ccgccagaga ggtattacgc agagaaaaag gcgatgagga tcggcactaa caggctaaga
     9901 ataaaaccgt gaacaattgc cgccgggacc atatccagcc cgccagtacg ttgaagaacg
     9961 ggcagggtga aatccattga tgtggcaccg cataagccca gtgcagtaga gcggctgcgg
    10021 cgaatcagcc cagggatcaa cataatagca atcagttcac gggccagatc attaaaaaac
    10081 gccgcgctcc cgattaccgg accaaaagat tcggtcaata aaataccgga aagagaatac
    10141 cagccgaaac cggaggccat tgccagcgcg gtattgatgg ggagatcaag aataaaggcg
    10201 ttaattaaac caccaattaa tgaactgaca accaccacca cggcgacaat cattccccgg
    10261 cgattaagga caatctgctt taaggtcatg ccattattgc gcaactgaat accaacgagg
    10321 aaaagtagca aaattaacgt gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt
    10381 ccactcagac caatggcaaa accaatcact actacgccgc acagttttag cgactccagc
    10441 gccatcgcaa tacgcgacgg gagtttttct tgctgatggt ggttgcgcca cggcaggcct
    10501 cgctccagcc acatcagggc ggcaatatta cacagtaaaa taacggtaat actgacggca
    10561 gaataatgca gaatcgccaa caggttactg gcgaggttat cgagaaacgc cagactgata
    10621 cccataaaaa agagaataag gtaaaccatc cagcttaata gctgattaat aacttttaac
    10681 gcagcttgtt ggcgaagcgg aatgaggtaa cctacaatca ggggaaccag aatgattaac
    10741 agcccagaaa acatgaaaac ccagtccttg caaagatgaa gtcgaaatgc gcgatgacac
    10801 actactgaaa gcggaaggac gagtaaagtt gcaattaaaa ggaaatgtta tgcataagga
    10861 gcagtagagt attcgttttc atttaaagat attcttgcgc tttaattaca aactgcaccg
    10921 atgttggtgg cgtcaaaatc gccgaggcgt tccctgaagg ccggggcagc ccacatggat
    10981 gtgggctgag ggcgcgtttt acagggatgt tacctcgcgc ccgacccggt agccgtaagg
    11041 gataagtcga gggcaccgcg cagcggcgat tttgttcgcc agagcccggg ggggcagggg
    11101 gcggcggcga ttggccgccc cctgcgcgct ccttgcgcca gtggcaatat gtggcttagc
    11161 tcatgaaagg agcgcaacaa gatgatgaat caacatataa caacatctta aaaaaaggcc
    11221 tgacattacg ccaggccttc tgcgttaatt aatcacgctt ttccagcagg gtccggtaaa
    11281 tcagaccacc gataatgccg ccgacaattg gcaccaccca gaagaaccac agttgttcta
    11341 atgcccagcc gccctggaag atagcaaccg cggtgctgcg cgccgggtta acagaagtgt
    11401 tagtcaccgg aatactaatt aagtgaatca gggttaaggc cagaccaata gcgatcggcg
    11461 caaaacctgc cggcgcgaat ttgtcggttg cgccgtggat caccaacagg aaacctgcac
    11521 tcaataccag ttcaactacc agcg
//
LOCUS       DAZOXR010000137        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZOXR010000137 DAZOXR010000000
VERSION     DAZOXR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.139"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUNN_003144"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUNN_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0577449.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUNN_003145"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUNN_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0577450.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUNN_003146"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUNN_003146"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0577451.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUNN_003147"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUNN_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0577452.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHUNN_003148"
     CDS             complement(3928..5214)
                     /locus_tag="ACHUNN_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0577453.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHUNN_003149"
     CDS             complement(5235..6026)
                     /locus_tag="ACHUNN_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0577454.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUNN_003150"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUNN_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0577455.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUNN_003151"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUNN_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0577456.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUNN_003152"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUNN_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0577457.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUNN_003153"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUNN_003153"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0577458.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUNN_003154"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUNN_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0577459.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHUNN_003155"
     CDS             complement(9882..10364)
                     /locus_tag="ACHUNN_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0577460.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUNN_003156"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUNN_003156"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR0577461.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZOXR010000138        11355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZOXR010000138 DAZOXR010000000
VERSION     DAZOXR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.140"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUNN_003157"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUNN_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR0577462.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUNN_003158"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUNN_003158"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0577463.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="ACHUNN_003159"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="ACHUNN_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0577464.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUNN_003160"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUNN_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0577465.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUNN_003161"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUNN_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0577466.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUNN_003162"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUNN_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0577467.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUNN_003163"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUNN_003163"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0577468.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUNN_003164"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUNN_003164"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0577469.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUNN_003165"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUNN_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0577470.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUNN_003166"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUNN_003166"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0577471.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUNN_003167"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUNN_003167"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0577472.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUNN_003168"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUNN_003168"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0577473.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="ACHUNN_003169"
     CDS             10898..>11355
                     /locus_tag="ACHUNN_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0577474.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3055 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAZOXR010000139        11304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZOXR010000139 DAZOXR010000000
VERSION     DAZOXR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.141"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHUNN_003170"
     CDS             <1..208
                     /locus_tag="ACHUNN_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0577475.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="ACHUNN_003171"
     CDS             300..2984
                     /locus_tag="ACHUNN_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0577476.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="ACHUNN_003172"
     CDS             2981..4069
                     /locus_tag="ACHUNN_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0577477.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHUNN_003173"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHUNN_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHUNN_003174"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHUNN_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHUNN_003175"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHUNN_003175"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0577478.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="ACHUNN_003176"
     CDS             8928..9479
                     /locus_tag="ACHUNN_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0577479.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHUNN_003177"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHUNN_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0577480.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2966 a         2772 c         2934 g         2632 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
    11041 ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
    11101 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
    11161 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
    11221 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccgattaca
    11281 taattaatag ctcacctttg tctg
//
LOCUS       DAZOXR010000140        11242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZOXR010000140 DAZOXR010000000
VERSION     DAZOXR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.142"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            3..308
                     /locus_tag="ACHUNN_003178"
     CDS             3..308
                     /locus_tag="ACHUNN_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0577481.1"
                     /translation="MLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="ACHUNN_003179"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="ACHUNN_003179"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0577482.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUNN_003180"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUNN_003180"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0577483.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUNN_003181"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUNN_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0577484.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUNN_003182"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUNN_003182"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0577485.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUNN_003183"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUNN_003183"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0577486.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUNN_003184"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUNN_003184"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0577487.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUNN_003185"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUNN_003185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0577488.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUNN_003186"
     CDS             complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUNN_003186"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0577489.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUNN_003187"
     CDS             complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUNN_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0577490.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUNN_003188"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUNN_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0577491.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11242
                     /locus_tag="ACHUNN_003189"
                     /pseudo
     CDS             10353..11242
                     /locus_tag="ACHUNN_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2648 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAZOXR010000141        11234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZOXR010000141 DAZOXR010000000
VERSION     DAZOXR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.143"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHUNN_003190"
     CDS             53..352
                     /locus_tag="ACHUNN_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0577492.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHUNN_003191"
     CDS             complement(472..651)
                     /locus_tag="ACHUNN_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0577493.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHUNN_003192"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHUNN_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0577494.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHUNN_003193"
     CDS             2090..2668
                     /locus_tag="ACHUNN_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0577495.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUNN_003194"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUNN_003194"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0577496.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHUNN_003195"
     CDS             4347..5945
                     /locus_tag="ACHUNN_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0577497.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUNN_003196"
     CDS             complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUNN_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0577498.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUNN_003197"
     CDS             complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUNN_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0577499.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7962..8933
                     /gene="manX"
                     /locus_tag="ACHUNN_003198"
     CDS             7962..8933
                     /gene="manX"
                     /locus_tag="ACHUNN_003198"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0577500.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8996..9796
                     /gene="manY"
                     /locus_tag="ACHUNN_003199"
     CDS             8996..9796
                     /gene="manY"
                     /locus_tag="ACHUNN_003199"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0577501.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUNN_003200"
     CDS             9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUNN_003200"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0577502.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10715..11173
                     /locus_tag="ACHUNN_003201"
     CDS             10715..11173
                     /locus_tag="ACHUNN_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0577503.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2762 a         2826 c         2833 g         2813 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggg ttatcggcat cgtgctgaac attggcctga
     6721 cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc
     6781 gactggtggc gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag
     6841 aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc
     6901 agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg
     6961 ttaacgaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc
     7021 gccgccatca tcaccggcag atggttaacc atccctactg cagtaattac cgcatccaac
     7081 gagaagacgg cgtcaaggat gacaatctgt gtgacgacca cccagaaact ggcgtagcct
     7141 ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct
     7201 ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa
     7261 tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc
     7321 cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt
     7381 ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt
     7441 tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg
     7501 acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa
     7561 aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca ggcgttaaaa ggcctgatgc
     7621 tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta
     7681 aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca
     7741 aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac
     7801 ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg
     7861 tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaaatacat
     7921 ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt gctattgtta
     7981 taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg ctgttaggcg
     8041 agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa acgctgattg
     8101 aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg tttctcgttg
     8161 atacatgggg aggcagcccg ttcaatgctg ccagccgcat tgtcgtcgac aaagagcatt
     8221 atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg gctcgtgatg
     8281 atgacccaag ctttgatgaa ctggttgcgc tggcagtaga aacaggccgt gaaggcgtga
     8341 aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc acccgctgcc gcagcaccaa
     8401 aagcggctcc aactccggca aaaccaatgg ggccaaacga ctacatggtt attggccttg
     8461 cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc aaagaaacca
     8521 atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt cgtaagacac
     8581 tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt gccaaaatga
     8641 ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt aatgctgtta tttaccaacc
     8701 caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt aacgtcggtg
     8761 gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt gatgaaaaag
     8821 atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga gctggaagtc cgtaaggttt
     8881 ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag taacgtattg
     8941 tgttgattat cactcagttt tcacacttaa gtcttacgta aacaggagaa gtacaatgga
     9001 gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag gtatgggatc
     9061 aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg tgggtatcgt
     9121 tcttggggat atgaaaaccg gtattattat cggtggtacg ctggaaatga tcgcgctggg
     9181 ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcttcta tcatttctac
     9241 cattctggtt atcgcaggtc atcagagcat tggtgcaggt atcgcactgg caatccctct
     9301 ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg ctttccagca
     9361 cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga tccacgtttc
     9421 ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg cgctgtctgt
     9481 tggtaccagc gaagtacaga acatgctgaa tgcgattccg gaagtggtga ccaatggtct
     9541 gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca acatgatgcg
     9601 tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat tcaccaactt
     9661 taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca tccaacttag
     9721 cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca acgatctcga
     9781 taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta ccaccgagaa
     9841 aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct tccagggttc
     9901 atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac cggcaattcg
     9961 tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc acctggagtt
    10021 ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg cgctggaaga
    10081 acagcgtgct aatggcgcag agatcgacga cggtgctatc aacggtatca aagtcggttt
    10141 gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac gtccggtatt
    10201 tgcagcactg ggtgccggta tcgcgatgag cggcagcctg ttaggtccgc tgctgttctt
    10261 catcctgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt atggttactc
    10321 caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac tgacggaagg
    10381 ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt aacaagtgga cacatgtcaa
    10441 catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg ttactactgt
    10501 ccagactatt ctggaccagt taatgccagg cctggtacca ctgctgctga cctttgcttg
    10561 tatgtggcta ctgcgcaaaa aagttaaccc gctgtggatc atcgttggct tcttcgtcat
    10621 cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta cactaccggg
    10681 gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc tggtactgat
    10741 tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca tgccccgccg
    10801 taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg atagcgttat
    10861 cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg gtgcgttaat
    10921 aacgacatgg ttattaagcg cactggctct gatgggtttt tatatattct ggatccgcgt
    10981 tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga ttgaatatag
    11041 ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat tagaacagcg
    11101 tcggctgtta atccgcgttc gaaatatcga cgatctggaa aaaatttata agcttctcgt
    11161 ttcaactcaa taagttatga atttagccaa agctatgttt agtgtatttt taataatcag
    11221 acatagctta ggct
//
LOCUS       DAZOXR010000142        11139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZOXR010000142 DAZOXR010000000
VERSION     DAZOXR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.144"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            57..983
                     /locus_tag="ACHUNN_003202"
     CDS             57..983
                     /locus_tag="ACHUNN_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR0577504.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(933..2126)
                     /locus_tag="ACHUNN_003203"
     CDS             complement(933..2126)
                     /locus_tag="ACHUNN_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0577505.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2262..3986
                     /gene="iucA"
                     /locus_tag="ACHUNN_003204"
     CDS             2262..3986
                     /gene="iucA"
                     /locus_tag="ACHUNN_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0577506.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3987..4934
                     /gene="iucB"
                     /locus_tag="ACHUNN_003205"
     CDS             3987..4934
                     /gene="iucB"
                     /locus_tag="ACHUNN_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0577507.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4934..6676
                     /gene="iucC"
                     /locus_tag="ACHUNN_003206"
     CDS             4934..6676
                     /gene="iucC"
                     /locus_tag="ACHUNN_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0577508.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6673..8010
                     /gene="iucD"
                     /locus_tag="ACHUNN_003207"
     CDS             6673..8010
                     /gene="iucD"
                     /locus_tag="ACHUNN_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0577509.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8016..10211
                     /gene="iutA"
                     /locus_tag="ACHUNN_003208"
     CDS             8016..10211
                     /gene="iutA"
                     /locus_tag="ACHUNN_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0577510.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10762..10905)
                     /locus_tag="ACHUNN_003209"
     CDS             complement(10762..10905)
                     /locus_tag="ACHUNN_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577511.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2799 a         2787 c         2882 g         2671 t
ORIGIN      
        1 cacaagttga aataatagta ttatattgaa aagacagaga ttatttcaga ggtgttatgg
       61 aaaactgggt tattcaggag ctaaaatcac tggatatagg ggactcaaga cttgataaaa
      121 gagtaaaaca tatcctcaat agcctcagtc gttcaccaga agaaagtatt cctgtttcct
      181 gccgtacctg gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg
      241 ctaataaaat tatggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa
      301 gagttttagt tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag
      361 gagttggtca caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca
      421 tcagtgagtc aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat
      481 taaaaaccag taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg
      541 tcagtgaaaa agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc
      601 gccaatgccc tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc
      661 tgtttgaacg cgcagagcag actcccggaa taaaagctga ctggcttgtc agaatgaaat
      721 ttaataacag agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa
      781 gaatcatgga gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc
      841 agcaagccag aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc
      901 tgatttactc cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata
      961 aatacagaca caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt
     1021 gcgacacctg ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg
     1081 tgactggtag cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt
     1141 tgataagcgg gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac
     1201 aaccatagct gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc
     1261 aggagcaggg ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc
     1321 gcagctactg cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc
     1381 gtgacgaact ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca
     1441 gccagtaata ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg
     1501 cactccggtg tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc
     1561 actggtgctg ccatcagtag ccccagtggc gccagaggat gaatggccag cattgctgcc
     1621 gcgcacagcg gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg
     1681 gccatgcgat tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt
     1741 gccgacacgg tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct
     1801 gttgttatgg cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg
     1861 ccatatcctc ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt
     1921 ccccaccacg gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat
     1981 acagacagac tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc
     2041 tgtccgatac cgagaagccc ggcactgaac gcaagtggcc agcaggacag tgttttttgg
     2101 ggcgtatctt cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt
     2161 atttgtaaaa ttaatttatc tgacaataac atttattgtt gataatgaga atcattatca
     2221 acataattgt tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa
     2281 aaccagccac agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag
     2341 actggaaact gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat
     2401 cgctccattt cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg
     2461 cacgtctggt cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta
     2521 ttattgataa gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc
     2581 agcgcgtgct ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg
     2641 ccgctctgcg tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac
     2701 acgctttcca ccctgcgcct aagtctcatg aaccgtttaa ccgacgggag gctgaacgct
     2761 acctgcctga tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa
     2821 tcgccggtga aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga
     2881 atgcgcctca gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt
     2941 ggcaggggga atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga
     3001 ttaaagactt aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct
     3061 actgtgccac cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca
     3121 tccgtactct gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa
     3181 ccgacggctg gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg
     3241 gctgggccgg gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc
     3301 gtgaaaatct gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc
     3361 aggccgcacc tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc
     3421 gcctcggtat cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag
     3481 tgctaaagcc gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc
     3541 aaaatattct tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc
     3601 agggcagcgc ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg
     3661 cggaaaatat tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta
     3721 actccacttt tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc
     3781 tgatggctcg tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct
     3841 gtctcaacta cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc
     3901 tgaacgatca taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta
     3961 acccgctgca ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg
     4021 gactgcgctg tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg
     4081 cggtgttgca ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga
     4141 tatttatcgc cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg
     4201 agccacaggc gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct
     4261 tctggcagtt accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt
     4321 ttgatgcaga gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt
     4381 accgccgtta cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt
     4441 ctgatgcaga acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc
     4501 aaagtggctc actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg
     4561 cattcccgct gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg
     4621 cggcggaaga ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc
     4681 tgctggttgg tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc
     4741 tgacacatta cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca
     4801 ccgataacca gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt
     4861 tcgacttccc acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg
     4921 aggtcggtct gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa
     4981 aatgttgtct gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca
     5041 ctactgcatt aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg
     5101 ctggctgtgg attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac
     5161 gctgctgatg cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat
     5221 gcaggatttg tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact
     5281 gagtgccagt gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca
     5341 tcctaaattc gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata
     5401 tgcgccagag tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat
     5461 gatctggcgc tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc
     5521 gcaggagttt acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct
     5581 accgctgccg gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga
     5641 ttttgtcgaa ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca
     5701 gtcgctgcgt accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct
     5761 gaccatctac aacacctcat gctaccgggg aatccctggc agatatatcg ctgccggacc
     5821 actggcttca cgctggctgc aacaggtttt tgcgaccgac gccaccctgg tgcaaagcgg
     5881 cgcagtgatc cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct
     5941 tgcccgggct ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg
     6001 ccgctggctg aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga
     6061 aaacaatcag ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg
     6121 gcttacgcaa ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg
     6181 tgtcgcgctt attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca
     6241 gcgtgttctg ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga
     6301 aatggactct ttgcctcagg aggtgcgtga tgccacatcc cgtctgagtg cggactactt
     6361 aatccatgat ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat
     6421 ggttcgtctt ggcgtacctg aaaggcgatt ttatcaactg ctggcagcag tgttgagtga
     6481 ttacatgaac aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc
     6541 acaaatcatt cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg
     6601 cagtcgcatg ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca
     6661 ggaatatgaa tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat
     6721 ctcagcattg ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa
     6781 catcctcatt tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc
     6841 tttctgaaag atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat
     6901 ctggtgaagc acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt
     6961 gaagagtttt ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt
     7021 cataccgttg aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt
     7081 cggggagaat attttgcccg caatatctgc cttggtacag gaaaacaacc ttatttacca
     7141 ccctgtgtga agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg
     7201 ccggacctga gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg
     7261 ttccttaatg cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg
     7321 cgtaataatt ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa
     7381 tatatttcag gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag
     7441 aaaatgacat cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac
     7501 caccgttttg aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta
     7561 acaaccctgg aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag
     7621 ggcagagaga gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg
     7681 ccacaaatac ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa
     7741 gtgcgtgatg acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc
     7801 aacgccagca tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga
     7861 tctgcacgta ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc
     7921 gcactgattc agtggcgcag cggcagccgg aaaaaaccgc agccggaggc tgcttcttta
     7981 actcactata cagcaaatat tcaggaataa cgatgatgcg caaaaagtat atgccccggg
     8041 ctcttggtcc gctgcttctt gtcgtgctgt caccagctgt cgcccagcaa aacgatgata
     8101 atgagatcat agtgtctgcc agccgcagca atcgaactgt agcggagatg gcgcaaacca
     8161 cctgggttat cgaaaatgcc gaactggagc agcagattca gggcggtaaa gagctgaaag
     8221 acgcactggc tcagttaatc cccggccttg atgtcagcag ccagagtcga accaactacg
     8281 gtatgaacat gcgtggccgc ccgctggttg tcctgattga cggtgtgcgc ctcaactctt
     8341 cacgttccga cagccgacaa ctggactctg tcgatccttt taatatcgac catattgaag
     8401 tgatctccgg cgcgacggcc ctgtacggtg gcgggagtac cggagggttg atcaacatcg
     8461 tgaccaaaaa aggtcagccg gaaaccatga tggagtttga ggctggcaca aaaagtggct
     8521 ttaacagcag taaagatcac gatgagcgca ttgccggtgc tgtctccggc ggaaatgacc
     8581 atatctccgg acgtctttcc gtggcatatc agaaatttgg cggctggttt gacggtaacg
     8641 gcgatgccac cctgcttgat aacacccaga ccggcctgca gcactccaat cggctggaca
     8701 tcatgggaac cggtacgctg aacatcgatg aatcccggca gcttcaactg ataacgcagt
     8761 actataaaag tcagggagac gacaattacg ggcttaatct cgggaaaggc ttttccgcca
     8821 tcagcgggag cagcacacca tacgtcagta aggggctgaa ttctgaccgc attcccggca
     8881 ctgagcggca tttgatcagc ctgcagtact ctgacagtga tttcctggga caggaactgg
     8941 tcggtcaggt ttactaccgc gatgagtcgt tgcggtacta cccgttcccg acggtaaatg
     9001 cgaataaaca ggcgacagct ttctcctcgt cacagcagga taccgaccag tacggcatga
     9061 aactgactct gaacagccaa ctgatggacg gctggcaaat cacctggggg ctggatgctg
     9121 agcatgagcg ctttacctcc aaccagatgt tcttcgatct ggctcaggca agtgcttccg
     9181 gagggctgaa caaccataag atttacacca ccgggcgcta tccgtcatat gacatcacca
     9241 atctggcggc cttcctgcaa tccagctatg acattaatga tatttttacc gttagcggtg
     9301 gcgtacgcta tcagtatact gagaacaggg tagatgattt catcgactac acgcagcaac
     9361 agaagattgc tgccgggaag gcgatatctg ccgacgccat tcctggtggt tcggtagatt
     9421 acgataactt tctgttcaat gctggtctgc tgatgcacat caccgaacgt cagcaggcat
     9481 ggttcaattt ttcccagggg gtggcattgc cggatccggg gaaatattat ggtcgcggca
     9541 tctatggtgc agcagtgaac ggccatcttt ccctgacaaa gagcgtgaac gtcagcgaca
     9601 gtaagctgga aggcgtgaaa gtcgattctt atgaactggg ctggcgcttt accggtgaca
     9661 acctgcggac tcaaatcgcg gcatattact cgctttccaa taagagcgtg gaaaggaata
     9721 aagatctgac catcagtgtg aaggacgaca ggcgccgtat ttacggcgtg gaaggtgcgg
     9781 tggacttcct gatcccggat actgactgga gtaccggtgt gaacttcaat gtgctgaaaa
     9841 ccgagtcgaa agtgaacggt caatggcaaa aatatgacgt gaaggaatca agtccatcga
     9901 aagcgacagc ttacattaac tgggcgccgg aaccgtggag tctgcgtgta cagagcacca
     9961 cttctttcga cgtaagcgat gcagagggta acgatattaa tggttacact accgtcgatt
    10021 ttatcagtag ttggcagctt ccggtgggaa cactcagctt cagcgttgag aacctcttcg
    10081 accgtgacta taccactgtc tggggacagc gtgcacctct gtactactgc ccgggttacg
    10141 gccctgcttc actgtacgac tacaaaggcc ggggccgaac ctttggtctg aactactcag
    10201 tgctgttctg acggggatgc ttttgcaaca agggtatgct gtaaacaaca tggtccttgg
    10261 tggccatgtt gtttacagcc agtataatat acagccttaa caagatattc cgtctccgga
    10321 atggactgtt caccactgaa taacattatt tctgttttat ggatctttga ctgaaattta
    10381 tcgcatatcc tcatttcatt gtccgtctga aaactaacct tacaagtaag cgtcgtctca
    10441 gcaccgtctg gcagatcctg aaattcctga gaggatgatc tgcgcccaat atcctgagcc
    10501 ggaactttgc tgcaatgaag gccaacgaaa aatgggggag tatgttatag agtacttgct
    10561 acagggtaaa aagaggtacc tgtcgctggt tctccgttca gtttaaccgg gagattttct
    10621 cctgcgcctg tcataaagtc cggtaatgga gattgttaat acgatggtgc acgatgcatt
    10681 gtcggtgccc ggcccggagg atgccccgta actacatacg gatcagggtt ggcaatatcg
    10741 aatggcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat gccacacaaa
    10801 ggaaacggtc tgaataacgc agtgatggag aacttcttcg gcacactgca aaatcgcatc
    10861 gtggtcgatc gctaattcgc cgtgaatcac tgaacgatgt tccatgcgaa cagatattcg
    10921 atcatatcga aatattaaac attacccctc cccgcattgg gcaaaaaaaa aacaaaaaac
    10981 gatcaattag tattgattca cataatctct catcattaat gagaacattg tcacatattt
    11041 acaatataga actgttactt ttagtaagct gttagtaacc aactgttact aataatttta
    11101 attcttaata ctttgttcat tcaataaatg agttgagag
//
LOCUS       DAZOXR010000143        11082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZOXR010000143 DAZOXR010000000
VERSION     DAZOXR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.145"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1225
                     /gene="narU"
                     /locus_tag="ACHUNN_003210"
     CDS             <1..1225
                     /gene="narU"
                     /locus_tag="ACHUNN_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0577512.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1307..5047
                     /gene="narZ"
                     /locus_tag="ACHUNN_003211"
     CDS             1307..5047
                     /gene="narZ"
                     /locus_tag="ACHUNN_003211"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR0577513.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5044..6588
                     /gene="narH"
                     /locus_tag="ACHUNN_003212"
     CDS             5044..6588
                     /gene="narH"
                     /locus_tag="ACHUNN_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0577514.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6588..7283
                     /gene="narW"
                     /locus_tag="ACHUNN_003213"
     CDS             6588..7283
                     /gene="narW"
                     /locus_tag="ACHUNN_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0577515.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7280..7960
                     /gene="narI"
                     /locus_tag="ACHUNN_003214"
     CDS             7280..7960
                     /gene="narI"
                     /locus_tag="ACHUNN_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0577516.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8039..8932
                     /locus_tag="ACHUNN_003215"
     CDS             8039..8932
                     /locus_tag="ACHUNN_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR0577517.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="ACHUNN_003216"
     CDS             complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="ACHUNN_003216"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR0577518.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10046..10615
                     /locus_tag="ACHUNN_003217"
     CDS             10046..10615
                     /locus_tag="ACHUNN_003217"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR0577519.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="ACHUNN_003218"
     CDS             complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="ACHUNN_003218"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR0577520.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10946..>11082)
                     /locus_tag="ACHUNN_003219"
     CDS             complement(10946..>11082)
                     /locus_tag="ACHUNN_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0577521.1"
                     /translation="GIRHIAINILTNDKVFKAGLRRKMRKAAMGRNYLASVLTGSGLS
                     "
BASE COUNT         2577 a         2892 c         2969 g         2644 t
ORIGIN      
        1 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
       61 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      121 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
      181 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
      241 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
      301 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
      361 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct
      421 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
      481 gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga tgaatgatat
      541 cgccagttca cgcgcctcaa ttgacgacca gctccctgtc ttacaacgcc tgcatctctg
      601 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
      661 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
      721 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata aattcggcgg
      781 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
      841 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
      901 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
      961 ccagataacc atttatcggg tgaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1021 agccatcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1081 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1141 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     1201 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     1261 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     1321 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     1381 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     1441 tgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     1501 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacca
     1561 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag
     1621 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgtgaagc
     1681 tctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     1741 gtgcttaagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     1801 acttaaccaa ctgattgccg ccgctaacgt ctggaccatc aaaatctatg gtccggatcg
     1861 cgttgccggt ttctcgccga tcccggcgat gtcgatggtg tcatacgctg ccggaacgcg
     1921 ttatctgtcg ctgttaggcg gcacctgttt aagcttctac gactggtatt gcgacctgcc
     1981 gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg ccggaatctg ccgactggta
     2041 taactccagt tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2101 ccacttcttt accgaagtac gttacaaagg caccaaaact atcgccatta cccctgacta
     2161 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     2221 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     2281 ctactttatc aactactgcc gccgctatag cgacatgccg atgctggtaa tgctggaacc
     2341 tcgcgacgat ggtagctacg tacccgggcg catgatccgc gcgtctgacc tggtagatgg
     2401 actaggcgaa agcaataatc cgcagtggaa aaccgtggcg gttaataccg caggtgaatt
     2461 ggtagtgccg aatggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     2521 atccattgcc gccggtaagg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     2581 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt
     2641 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     2701 caacacctgt ccggtggtca gcgtttatga tttggtgctg gcgaattacg gcctcgatcg
     2761 cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     2821 ctggggtgag caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgcgaatt
     2881 tgccgatacc gcccataaaa cgcatggacg ctcaatgatt atcctcggcg ctggcgtcaa
     2941 ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga tcttctgcgg
     3001 ttgtgtcggg caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc
     3061 acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgcccac cgcgccagat
     3121 gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     3181 tcaggagtta ctttcaccgc ttgccgatgc cagtaagtac agcggtcatc tgattgattt
     3241 caacgttcgc gccgaacgca tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     3301 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     3361 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca
     3421 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     3481 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     3541 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg aaggcaagct
     3601 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     3661 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     3721 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     3781 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     3841 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     3901 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     3961 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4021 gatggacaaa cttggcaatg gtggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4081 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4141 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     4201 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     4261 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     4321 aattatctcc agccccacat ggtccggtct ggaaagcgat cacgtctcct ataatgcggg
     4381 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     4441 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     4501 cgacacccgt agcgtcagtg agatgcgcca gatcccgcca aacggcttcc cggaaaaagc
     4561 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     4621 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg
     4681 agaactgacc attgtcgata acgactgggt ggaagtcttt aacgccaacg gcgcgctgac
     4741 ggctcgtgcg gtggtcagcc aacgtgtacc gccgggtatg accatgatgt atcacgccca
     4801 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     4861 ctcggtcacc cgtgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc
     4921 ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg
     4981 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5041 aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc tcgacaaatg tatcggttgc
     5101 catacctgtt cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca
     5161 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     5221 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatacgccc gcgtctgggc
     5281 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     5341 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     5401 atccctactg ctcgcccgcg ttcgctgatt gacggtaagc ggatggacaa agtgatctgg
     5461 gggccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5521 ttcgaggcca tgcaaaagga gatgtacggg cagtttaaaa acaccttcat gatgtacctg
     5581 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     5641 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     5701 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     5761 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     5821 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt
     5881 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     5941 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6001 gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6061 ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg
     6121 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     6181 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     6241 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     6301 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     6361 gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg
     6421 accagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     6481 tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag tagccgtatc
     6541 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     6601 aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg
     6661 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     6721 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     6781 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     6841 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     6901 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     6961 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7021 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7081 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7141 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     7201 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca
     7261 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     7321 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta
     7381 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     7441 tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc
     7501 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     7561 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcagtct
     7621 gacaaatcag cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt
     7681 gctgatccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     7741 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     7801 gtcagagatg ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac
     7861 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     7921 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     7981 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8041 gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8101 cggtgttgtt ttccccgccg ataatcttag cgaagcacaa atgcagctta tcgcccgcga
     8161 gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     8221 ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     8281 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg
     8341 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     8401 tacgccgggc tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt
     8461 gcatcttact gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca
     8521 ttctaaagtg atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct
     8581 taatgcgctg accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat
     8641 ccgtccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg gtattgtgga
     8701 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     8761 attgccccac gatggcaacg tgttgtgtgt taaaggccat caggggcgcg cattggggcg
     8821 tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     8881 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     8941 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9001 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9061 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9121 accaaactgt tcctgcatca cggcatataa cgacgccaca tccggtagat ttcgctgctc
     9181 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc
     9241 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
     9301 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat cgaagcggta
     9361 catcggctgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
     9421 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
     9481 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt ccagttccac
     9541 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
     9601 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
     9661 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
     9721 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
     9781 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
     9841 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
     9901 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
     9961 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10021 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10081 cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg agcaatccca
    10141 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac ctccacgcgt
    10201 ggcaattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    10261 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc
    10321 ggtgcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    10381 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    10441 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    10501 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    10561 tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcag gttaagcatt
    10621 acatgctgta accgggtttc tttatcaaag cctccatttg gggctcgatt tcggcatccc
    10681 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    10741 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    10801 gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg cggcatgatt acgctccttt
    10861 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca tgtgtccggt aaagcgttta
    10921 tacgacaata atgcccaggg caagattacg aaagcccgct ccccgtaagg actgacgcca
    10981 ggtagtttct gcccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11041 tatcattcgt caaaatatta atagcaatgt gccgtatccc tg
//
LOCUS       DAZOXR010000144        11044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZOXR010000144 DAZOXR010000000
VERSION     DAZOXR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.146"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            203..931
                     /gene="gadW"
                     /locus_tag="ACHUNN_003220"
     CDS             203..931
                     /gene="gadW"
                     /locus_tag="ACHUNN_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0577522.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1294..4407)
                     /gene="mdtF"
                     /locus_tag="ACHUNN_003221"
     CDS             complement(1294..4407)
                     /gene="mdtF"
                     /locus_tag="ACHUNN_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR0577523.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4432..5541)
                     /gene="mdtE"
                     /locus_tag="ACHUNN_003222"
     CDS             complement(4432..5541)
                     /gene="mdtE"
                     /locus_tag="ACHUNN_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR0577524.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5649..5927
                     /locus_tag="ACHUNN_003223"
     CDS             5649..5927
                     /locus_tag="ACHUNN_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577525.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5928..6455)
                     /gene="gadE"
                     /locus_tag="ACHUNN_003224"
     CDS             complement(5928..6455)
                     /gene="gadE"
                     /locus_tag="ACHUNN_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0577526.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7254..7826)
                     /gene="hdeD"
                     /locus_tag="ACHUNN_003225"
     CDS             complement(7254..7826)
                     /gene="hdeD"
                     /locus_tag="ACHUNN_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0577527.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            8081..8413
                     /gene="hdeA"
                     /locus_tag="ACHUNN_003226"
     CDS             8081..8413
                     /gene="hdeA"
                     /locus_tag="ACHUNN_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0577528.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8529..8855
                     /gene="hdeB"
                     /locus_tag="ACHUNN_003227"
     CDS             8529..8855
                     /gene="hdeB"
                     /locus_tag="ACHUNN_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0577529.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8919..9566
                     /locus_tag="ACHUNN_003228"
     CDS             8919..9566
                     /locus_tag="ACHUNN_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR0577530.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9608..10138)
                     /locus_tag="ACHUNN_003229"
     CDS             complement(9608..10138)
                     /locus_tag="ACHUNN_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0577531.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(10294..10860)
                     /gene="slp"
                     /locus_tag="ACHUNN_003230"
     CDS             complement(10294..10860)
                     /gene="slp"
                     /locus_tag="ACHUNN_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0577532.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3024 a         2500 c         2531 g         2989 t
ORIGIN      
        1 ttataaacat aagctatacg ctgtgcgaaa atatattcgt gctgcattta cttattatca
       61 attaactgtt atgcaaaact actttgtgga taaattttgg tcctaccaaa tctggcagtt
      121 ttttcgctaa gaaacagtct ggcaacattt cattagtata ctgaaattga aataatcgca
      181 gtatgaaata taagggataa tcatgactca tgtctgctcg gtgatcctca ttcgtcgttc
      241 attcgatatt tatcatgaac agcataaaat atcgctgcat aacgagagta tcgtgctgct
      301 ggagaaaaat ttggcagacg attttgcgtt ttgttcaccg gatacgcgac gactggatat
      361 cgatgagctg acagtttgcc attacttaca aaatattcgt cagctaccac gcaatttagg
      421 gttacatagc aaagaccgtc tattaattaa ccagtcaccc cccatgccgc tggtgacggc
      481 gatttttgat agcttcaatg aatccggggt aaattcaccg atactgagca atatgctcta
      541 cctttcctgt ttatcgatgt tttctcataa gaaagaactg atccccttac ttttcaatag
      601 catcagcact gtttcaggaa aagttgaacg ccttattagc tttgatatcg ccaaacgttg
      661 gtatctgcgc gatatcgcgg aaagaatgta taccagcgag agtctcataa aaaaaaagtt
      721 gcaggatgaa aatacctgtt tcagtaaaat attactcgct tccaggatgt cgatggccag
      781 acgattactc gagttacgtc aaattcctct gcatactatt gcagaaaaat gtggctatag
      841 cagtacgtcg tactttataa acacatttcg acaatattat ggtgtaacgc cacatcagtt
      901 tgcgcaacat tcgccaggta ccttttcctg acatattttg catttgaata ttggtcagga
      961 tctcacacct gcttcatgtg aaactcttcc ctgatgattt ctgccgggct accggctagt
     1021 tctctttcgc agtttgccag gcggtatagg tattttcagg aggaaaagac tcggcatgtt
     1081 tgggattatt aagctgacaa ttcatacgat tacgggaatg tgggtcagta ttgtgctgtt
     1141 caaattgatg acgaatggct ggagtggttt ctactttcag tgctgtgtac taagcctggt
     1201 attcctgacg gttagctggt tgttgagtgg tgaatggtta gcaggaaaga gtaaggctga
     1261 accttcacgt tcaaccttac tctcatttac acgttacgct tttttaaagc gggcaaagag
     1321 atgttccact acaacgaaaa agaccggaac gaagtaaatt gccagcactg ttgcggcaaa
     1381 catcccgccc atcacgccgg tacctaccgc gttttgcgcg ccagaaccgg caccatgact
     1441 gataaccagc ggcagcacgc cgagaataaa ggcgagagag gtcatcagga ttgggcgtaa
     1501 acgcatccgc gccgcttcga tgattgcctc tatcggcgtt ttcccttctt tctgcatcat
     1561 ctcaacggca aattcgacaa tcaggatggc gttcttggcg gaaagcccga tggtggtcag
     1621 caaaccaacc tggaagtaga cgtcattact taagccgcgc agatcggtgg ccagtaatgc
     1681 gccaacgacg cctaacggaa caaccaacat caccgagaac ggaattgacc agctctcata
     1741 gagtgcggcg agggcgagga acaccacgac cagtgaaatg gcatacagtg caggagcctg
     1801 gtttgaggat aacgcttcct gatacgacag tccggtccat gagtagccga cgcctgccgg
     1861 aagtttagcg accaggtctg ccataaattt catagcgtca ccggtacttt tcccggccgc
     1921 cgcttcacct aaaatctcca ttgacgggat gccgttgtag cgttccaatc gcggtgaacc
     1981 ataggtccat tcggtagacg agtaggcaga aagcggtgcc atcgtgccag aggcgttgcg
     2041 tacataccat tggttgatgt tatccggcaa catacggaac ggcgtgcctg cctggacata
     2101 cacttttttc acccgcccct ggttgaggaa gtcgttcacg tagctgctgc cgaaggcggt
     2161 ggaaattgtc tggttgatat cagacagcgc cacacccatt gcctcagctt tcgcggcgtt
     2221 gacgttcact ttgaacatcg gcgtatcttc caggccgttc gggcgtaccc cggtgacctg
     2281 gtcaggcgat tgcgctgcca gtgataacag ctcgtttcgt gcctgggtta gtttttcgtg
     2341 ccccaggtta ccgttgtcca gcagttccat atcaaaacct gacgcggtac ccagttcagc
     2401 caccgcgggt aagttgaacg ggaagacgac ggctttattg atactgctta acgcaatcat
     2461 tgcccgctga atgatcgcgg taaccgagtt ttcctcaccg acacgttcag accacggctt
     2521 gagactgata aacgccaggc cgttgttttg cccctggccg ctgaagccaa agccgccaac
     2581 ggtaaacacc gactgaacat tatctttctc ttttgtaaga taataatccg tcacctgttg
     2641 cagcactttc gtggtgttaa ccatggtggc accggaaggt aactgcgcgg tggtcataaa
     2701 taccccctga tcctcttctg gtaagaaaga ggtcggtgta cgcaggaaca gcaccgccat
     2761 cccggcgcaa atcagcaggt agaccaccat gtagcgaccg gtacaacgca acagcgagcg
     2821 ggtgctatcg gtatagtgct gagttgattt ttcaaacagc gtgttgaagc gtgcgaacag
     2881 ggcgttaggt ttgtgaccgc cttccggcgc ggctttcaga atggtggcgc acagggcagg
     2941 ggtcaggctc attgccacaa atactgaaag cagcatggag gagatcagcg tgatggagaa
     3001 ctggcggtag atctccccgg ttgcgccgct cataaaggcc atcggcataa acactgcgga
     3061 aagaacaaca gcaataccga ccagcgcacg ttggatctgc cccatcgatt tatgcgtcgc
     3121 ttccttcggt ggtagcttat cttccgcaat gacacgctcg acgttctcca ccaccacgat
     3181 ggcgtcatcc accagtaacc ctatcgccag caccatcccg aacatagtta acgtgttgat
     3241 ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga ataaccaccg gtacggcaat
     3301 cgtcgggatg attgtggcac ggaaattctg caaaaacaga tacatgacca ggaagacgag
     3361 gatgatagcc tcaaccagtg ttttaaaaac ttcctgaata gaaatttcga taaacggcgt
     3421 ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa taggctgata agcggttcag
     3481 ctcctctttg actgcccgcg aggtatccag ggcgtttgct ccggtagcca gtttgatggc
     3541 gatcccggca gcaggtttgc cgttatagcg cgccacggtg gaataatctt ccgctccaag
     3601 ttcgacgcgg gcgacatcac gcagcagcac ttgcgaacca tcttgctgaa ctttcaacag
     3661 gattttgcca aattcttccg gcgtttgcag acgcgtctgc acaatgatcg aggcgtttag
     3721 ttgctggtct gccgcctgtg gcatgccacc cagttgacca ccggaaatct ggttgttttg
     3781 caccttaatc tgggaaataa catcggaagg taccagattg tatttattga gtttttgcgg
     3841 gtccagccag atacgcatgg cgtactcgga accaaagagc tgtacgctac caacgcccgc
     3901 ggtacggctt agcgggtctt tgatattaga cgctacatag tccgcgatat cgtactggtt
     3961 gaggctgccg ttatcagaaa taaacgccgc taccatcaga atattactgc tcgacttatc
     4021 aacgctaatc ccctgctgct gcaccgcttc aggtaatgaa ggcatagcga gttgcagttt
     4081 attttgcacc tgaacctgtg cgatatcagg agatgtccca gtctcgaagg tcagagtgat
     4141 agaggcatta cccgccgcat cactggttga agacatgtac atcaggccat caagcccatt
     4201 catattttgc tcaatcacct gagtgaccga gtcttctacc gtttgcgcat cggcacctgg
     4261 ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc ggatactgcg caaccggtaa
     4321 gttcatgatc gccagaccac ctgcaagcat cataataatg gcaagtaccc aggcaaaaac
     4381 cgggcgatca ataaaatagt tagccatgaa agtcccctta agcctgcaac gttattgttt
     4441 cgattcggtg ctggcgtttt cctggctgga ggaaattgct cgtgctttga tacccggacg
     4501 aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc tgcaagccag aggtgacaac
     4561 ccactggtcg ccgatggctt tgctggcttc aatttcgcgt agctgcacaa catcgtcttt
     4621 atccagaatg agcgccgttg ctttaccctg ggcgttgtga gtgacgcctt cctgcggcac
     4681 cagtaataca ttctggcggc taccttcatc cactaatgcc gtgacgtaca tgccaggcag
     4741 caagtcacca tttgggttgg ggaaaatcgc ccgtaacgtc acggagcccg tggtttcatc
     4801 caccgtcggg tcggagaatt tcagtgtgcc ggtctggctg tagcgtttac cattttccag
     4861 attgagctgt actggcgtac tgccctgaac ctgtttgatt tgcccactgg cgacctcttc
     4921 tttcatgcgc aagaaatcct gcaccgactg cgtgagatcg acataaatcg ggtccagacg
     4981 ttgtacggta accagcgaat ctgcctgatt agcggtaacg agtgcgccga cggtcaccga
     5041 cgatttcccg ctgacgcccg taatcggcga ggtgacattc gcgtattgca gattgatcgt
     5101 cgcctgttca acagccgctt tggcgacggt gacattggct tctgcttcat tcagctgggt
     5161 gcgcgcggtg tcgtaatcct gacggctaac gtagttggtc ttcagcaacg atgcctggcg
     5221 gttaaaggtg atgcgggcat tgctggcggt agagagcgct ttcgccagcg agcctttggc
     5281 ggagtttagc tcggcctgta aaggtgcagg atcaatctga tacagcgaat cgccctggtt
     5341 cactttatcg ccttcgataa agttgcgttt aatgataata ccgcccacct ggggacgtat
     5401 ctcggcaact tcataaggaa cggttctacc gggcaattcg ctcaacacat tgaccgaacc
     5461 gggggagagt gtgacgacac cgacctcagg cgtcatggcg gcggcgtttt ccgccgattt
     5521 gtcatcgcag gcggtgagca tcgcgccgca gaataacaac ggtattaaca gctttcttct
     5581 tctgttcatt ttagtccctg aaaattcttg agaaataacg gttttttaaa ttgcgcgcga
     5641 ggagagatat gagggattta cagaccagcg gcatagtggg gttgtcagcc agtaaggtgg
     5701 gatacaggca cagtgatcga catggtgagg tcaacgacaa aaaaaatgtt gccgttctgc
     5761 caacagttcc tgccagcatt cgggcaacga agggttctac tggtggatac acatacctgg
     5821 ggaataaagt cgttttagcc tctcctttag taatcaccgg gggtaatgaa atcagcatct
     5881 gcatccctcg tcatgccagc catcaatttc agttgcttat gtcctgacta aaaataagat
     5941 gtgataccca gggtgacgat gtcgctcata cgtcttagtt tcagcttgta cataatgttg
     6001 aatttctgaa tctttaatgt ttgttgcgaa atattgagcg tcgacgtgat attgctttca
     6061 tttttatttt tcagaatcag ttgcataatt ttgaactgat tatgtgaaaa acataattgt
     6121 gaattcttat ggggcaagtg tttaccataa gtaatctcaa gaagattttt gatactttct
     6181 ttgcggctaa caaaataaat aggcgcttta gcttttagtt tactgatgtg ataaggtgcc
     6241 aaaacaataa ttctctcggc atctaatttc tccagaaatt taatcgcttc ttcatcaagg
     6301 atatgattgt gataggtatc aatgataacc ttgaagggtt tattgcctac ttttttgatc
     6361 tctgacaggg aattgatttt tatcatttcg tgattatctt tcaactgcca aaagccctgt
     6421 aaaagaaaag aatctttcgt catgagaaaa atcataactt gctccttagc cgttatcgtt
     6481 tcgaacatgt catccttttc attatttaca tccttgtccg aatcgttgtt caatatagta
     6541 aacgcctata agaactttac ccttcgtgaa ctaatacgtc tattaacact aatcaatact
     6601 tcagatttat ccatcacgca ctccatatat tgatcctaag taagattcct ggttgttatc
     6661 agcttgtaaa tagtaaacaa aaagttaacg tttgcggatt actgcccaag aataagttat
     6721 caataaccag tttgtgatct ctgaagaata ttactaaagt taaaatgtaa tccgatttaa
     6781 atatcgagtc tccttgtttc gacttaagct ggcaattgga ttgccagctt tcttttaacg
     6841 cattgaaatt acctgagtaa ataacgcatt catgtcttca ggtttttata tagcgtctgg
     6901 agacacggat atttatgcaa tgtaatcaac gccagttgca aaaaaatgaa cccggattac
     6961 ttatttaacc gcccatagca tcaacgctta cggaactctt tgttcacttt cccacagcac
     7021 ttttaaaaca ttaaacctga ctacggaaaa tatcagccat gataatgttt gaaatggcta
     7081 atttgccatg gagtgaaaaa aattagatga aattcagtag gttgaaataa tcactagcag
     7141 gtaattattt caatgatagt gcgcaattga tctacaacac tccgtagcgg agagagtatt
     7201 aatcggatca tagtcatatc aagtgactat gatccgggtg acaaccggga taattattgc
     7261 tgcttaacga acaaactggc gaagctgaac aggctggcgg cgctaaatat cagttcaatt
     7321 cccaccagtg tggaaaccag cgttacagac accatcggcg ttgcaccaag gaatatccag
     7381 gcaatgacga tatccagcac accaataacg agctgtagcc agctgccttt cattgaacgc
     7441 tgacgatacc aactcatcag gcgaataacc cctgcaacac agaacaaacc ggcaataaat
     7501 gccgcaatgg caaaaatgcc cagctccggt gcgcggatga agaaatagcc gatcaataaa
     7561 taggcgactg cgacgaggaa accggataat accggccaga aattatgact gcggttgctg
     7621 aataacccga caataagcgc aatgcccgag cagattaata atgcacccac tactgtgctt
     7681 aaaatatcgc cagagacgaa cgggaaactg atacacagca acccgacgat aaacagtagc
     7741 acggcaataa actggattgc tctgcgatgt tttttaagca tctccagatc aaacttcaaa
     7801 attgttgcct tatctatata taacatagaa ccaccctata aaattaagaa gaaaatcccc
     7861 tgctatcaat ctatgccaaa aacgcgtcta agaatgtagt cgatttaata aaaatttcct
     7921 aattacagta tctgatgcat ctgtaactca ttgtattgaa ataaaaatat ctgattttga
     7981 tattttatat caacatgaca tatacagaaa accaggttat aacctcagtg tcgaaattga
     8041 ttcgtgacgg ctctttcact ttatagttga ggatattacg atgaaaaaag tattaggcgt
     8101 tattcttggt ggtctgcttc ttctgccagt tgtgagcaat gcagcggatg cgcaaaaagc
     8161 agctgataac aaaaaaccgg tcaactcctg gacctgtgaa gatttcctgg ctgtggacga
     8221 atccttccag ccaactgcag ttggttttgc tgaagcgctg aacaacaaag ataaaccaga
     8281 agatgcggtt ttagatgttc agggtattgc aaccgtaacc ccagctatcg ttcaggcttg
     8341 tactcaggat aaacaagcca actttaaaga taaagttaaa ggcgaatggg acaaaattaa
     8401 gaaagatatg taattccggg aatgcgttac atcgtacttc cttgcatatt gaacaggccg
     8461 gaatatcttc tttaaaagca gctattcctc ctgttcatat ataatctcta tattgaatgg
     8521 gttacaaaat gaatatttca tctctccgta aagcgtttat ttttatgggc gctgtagcgg
     8581 ctttgtcact ggtgaacgca caatctgcgc tggcagccaa tgaatccgct aaagatatga
     8641 cctgccagga atttattgat ctgaatccaa aagcaatgac cccggttgca tggtggatgc
     8701 tgcatgaaga aacagtatat aaaggtggcg ataccgttac tttaaatgaa accgatctca
     8761 ctcaaattcc taaagtgatc gaatactgta agaaaaaccc gcagaaaaat ttgtatacct
     8821 tcaaaaatca agcatctaat gacttgccga attaatgagg tacaagtaaa aaggagtagc
     8881 aagttgagcc atcttgctgc tcctttttgc atttttatat gacagcagaa tttattatac
     8941 gtcttatact cgcggcaatt gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca
     9001 aaggagcagg gttacgcaca catgtattaa ttggcatggg aagcgccctg tttatgattg
     9061 tttcgaaata tggttttgct gacgtgctgt ctttagatca cgtcggactc gaccccagcc
     9121 gtattgctgc tcaggtggtg acgggcgtcg ggtttatcgg tgcaggtaac attctcgttc
     9181 gtaaccaaaa tattgtaggt ctgacgacgg cagcggatat ctgggtgacc gccgccatag
     9241 gtatggttat tggcagcggt atgtacgaac tgggtattta tggttcagtg atgaccttgt
     9301 tggtgctgga agtcttccat caattaacct tccgcctgat gaacaaaaat taccatttac
     9361 agctgacatt agtgaacggt aatatggtat caatgctcga ctggtttaag cagcaaaaaa
     9421 taaaaactga tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc gccattgata
     9481 tccagcttca tgccacaacg tcgatagaag acctgttgcg attattaaaa ggtatggcag
     9541 gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg tagccagact caccgtaagc
     9601 ctgaaattca caccagataa tcaatatgct gatggcgtac cagctcattg atccgcttaa
     9661 gattgaggat catcatgata ttgtgtttat ggcagtagaa cgtttttagc gatattttca
     9721 gtttctccag aatatcttca gttgtcattt gattcgcaat gcagctcaga acctgatgtt
     9781 gcttgcgcga aagtgacaca ttcgcttcag gcacagagca gtaactgcca ttgatgagtg
     9841 cgacaaaatc aatgatagtg atagtccgcg gaacaaaggt caccggtact ttccccagac
     9901 agcgtttaat attaaagggg gagaaaacaa tgacatggac aggttttaat cgttcaattt
     9961 gagtgagaaa attagaatga aatacattat tcattaatgt atcaataatg acgaaggcat
    10021 tctgatgcaa cattacgtcg atctcttctt cgttctgtat atgaacgaca ttacctttac
    10081 tcagaatgtt tttcatcgcg gtgaagaaca tcgtatccct ggtaattata agaaacataa
    10141 ccgactcctg gtgcggacat taactttata atcaatttgc caaaggtaat taacaggaca
    10201 gggcttataa taataatagt tagcttactg tatattaatt ttatgtaata catgatgcag
    10261 atgctgagaa agcatctgca tctttcggtg gtgttatttg accagctcag gtgttacctg
    10321 actcaccgca ttggtgtagt aaggcgcacc ccagcccggt tccggccaga atgcaccata
    10381 gccgtaatcc cacaggttat aggtggtatt aaccacttct ctcaaatgcc acacctggat
    10441 gccctgcata ttcacttcca ggaagttata cgggacttta ttgataaagc caggttgttc
    10501 accctgaatg gtaccgagga tggtaacaaa gtgattacga tagttcactg ggtcaaggaa
    10561 gccgctttgt ctggcgagca gtcggccctg atagttggct tcaatatcag gtttcgcata
    10621 gctatccaac ggtaatacag cgatttctaa caacgtatcc gttttgccat tgataacgtt
    10681 gataaccttc ccaccaaagc gcgcttgttg accaacatat aaccccggct ggttatgaac
    10741 agcaacaaaa cttttttgaa tatcaggttg gttattgcct ttgatatttt gcggaattga
    10801 actacatgcg gcaagcaaaa atgaaaggct gaggatgagt gcaccttttg tcatgttcat
    10861 gttactatcc ttatcaacaa atattaaaac cataatagat atttctttat agttttcatc
    10921 tgattctgag gcgatatgcc tgatagtaac gtaaacctgt ctgatgttta tatccctttt
    10981 gtttagttca attccattga ttttggggcg tggcatgagt atccatcgcc ccataatata
    11041 atcc
//
LOCUS       DAZOXR010000145        11003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZOXR010000145 DAZOXR010000000
VERSION     DAZOXR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.148"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..280)
                     /locus_tag="ACHUNN_003231"
     CDS             complement(<1..280)
                     /locus_tag="ACHUNN_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0577533.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(447..1187)
                     /locus_tag="ACHUNN_003232"
     CDS             complement(447..1187)
                     /locus_tag="ACHUNN_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0577534.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHUNN_003233"
     CDS             complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHUNN_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0577535.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHUNN_003234"
     CDS             complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHUNN_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0577536.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            3110..3208
                     /locus_tag="ACHUNN_003235"
                     /pseudo
     CDS             3110..3208
                     /locus_tag="ACHUNN_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHUNN_003236"
     CDS             complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHUNN_003236"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0577537.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHUNN_003237"
     CDS             complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHUNN_003237"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0577538.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHUNN_003238"
     CDS             complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHUNN_003238"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0577539.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7170..7601
                     /locus_tag="ACHUNN_003239"
     CDS             7170..7601
                     /locus_tag="ACHUNN_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR0577540.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7624..8202
                     /locus_tag="ACHUNN_003240"
     CDS             7624..8202
                     /locus_tag="ACHUNN_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0577541.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8203..8910
                     /locus_tag="ACHUNN_003241"
     CDS             8203..8910
                     /locus_tag="ACHUNN_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR0577542.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8898..9575
                     /locus_tag="ACHUNN_003242"
     CDS             8898..9575
                     /locus_tag="ACHUNN_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0577543.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9569..10246
                     /locus_tag="ACHUNN_003243"
     CDS             9569..10246
                     /locus_tag="ACHUNN_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0577544.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10218..10931)
                     /locus_tag="ACHUNN_003244"
     CDS             complement(10218..10931)
                     /locus_tag="ACHUNN_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0577545.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10928..>11003)
                     /locus_tag="ACHUNN_003245"
     CDS             complement(10928..>11003)
                     /locus_tag="ACHUNN_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0577546.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2920 c         2740 g         2716 t
ORIGIN      
        1 ctcgtgaacg acgccctgag ttatggattt cattatcaat cgcagaaagt cgttgataaa
       61 ccgggaacag tttttgatac agttcctgac cggctttatt gaggctgata ttattatctt
      121 tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc ggcaatgctt ttaccaaatg
      181 gcgaagccgt catatggatt ttttctgccg cccgggcaat attattggtt tgcgctaata
      241 aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat cccccctccc cgcatccttg
      301 tccgcttaac cgtttcagtc agcctatcct tgatgaaacc gcgagcaaag ataggtgatt
      361 acgtcatggt tttacagaaa attacagaaa aaggaggcaa tatcgggtaa aggcattagc
      421 ccgacgaata cgtcgggcta caagtattat tgtgctgcag gtgttttagc gggttgttga
      481 tccacaggtt ctaactggaa gaccacatcg acctgatcat caaactgaat agcggcctgc
      541 tcgtaagttt cctgggcaga caccggcgcg gcatcggctt tcatcatccg caccattggg
      601 ctgggctgat agttggaaac atggtagcgc acgctatata ccggccccag tttacgatga
      661 aagccgttcg ccagttcctg cgcctgatga atcgcgttat caatcgctgc cttacgcgct
      721 ttgtctttat aggcatccgg ctgcgccacg cccagcgaca cagaacgaat ttcgttaaga
      781 cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt ctaactgacg gagcgtgact
      841 tccaccgttc tcacagcgcg gtagcctttc aggatacttt taccatcctg ataatcataa
      901 tctggctggg tgcgtaagtt cgctgagctg atatcttttt tcgcgatctg attgagttca
      961 aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt tcttggctgt agcggcatcc
     1021 ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt ctggcaccgc atccacactt
     1081 gccgtaccgg aggtgacaat atgcggtcca tccggcaatt cgttagcctg cgctgccatc
     1141 ccgctaatac ccattaatgc cgccagggcg ataactttga acttcacgtt agtctctccg
     1201 tgttaattga cttgcccaaa attcaggacc tctggaggca agcttagcgc ctctgtttta
     1261 tttttccatc agatagtgcc taactgaaca aggcttgtgc atgagcaata ccgtctctcg
     1321 ccagctgcaa ggctataaac cacatgacac atcccactac cagattgata atgcgctgtg
     1381 attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag aatagccaga ccaaagaacc
     1441 acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg ttttggttcg acgtcaagct
     1501 gcccgccaag gctgcccagt acaacaaaag tatccaggta aacatgcgga ttcagccagg
     1561 tcactgccag catggtggcg ataattttcc atctgccttg cttcaggact tcggcactag
     1621 ctaactcaat attactactc atcgctgttt taaaagcgcc aaaaccatac cacagcaaga
     1681 aggctacgcc gccccaggtg accagcgcca gcaaccacgg cgactgcatc aataacgcgc
     1741 tgccaccaaa aatcccggcg caaatcagga ccaaatcgct gatagcacaa agtaaggcaa
     1801 tcataatgtg gtactgacga cgaatgccct gattcatcac aaaagcattt tgtggaccga
     1861 gcggcagaat catagctgcc ccaagagcaa taccttgaaa gtaataagaa aacacgttgg
     1921 atattccgaa ttaagtatct gatgcagaga ctatatcgcg ggattgttat tagcggaaat
     1981 tgataatatt aattggcaat cagaataact aataagagag aggccagaga gtattatctg
     2041 gcctcagcgt tgattacgca gctttgtctt ctttcacccg cttaaagttc acatccattt
     2101 gcgggtacgg gaagctgata ccggcggcat caaattcacg tttaatacgc tccagcacat
     2161 cccagtacac gttttgcaga tcgccgctgt tgctccagac gcggaccacg aaattaatcg
     2221 acgatgcacc aagttcgttc agacgcacag tcatttcgcg atctttcaaa atgcgatctt
     2281 cagactggat aatattggtc aggatctgct taacctgatc gatatcggaa tcatacgcca
     2341 cgccaataat aaattcgtta cggcgaactg gctcgcgaga gaagttaata atatttccgg
     2401 caataatttt accgttcgga ataacgataa ttttaccgtc tgcagtacgc atggtggtgg
     2461 agaaaatctg cacactcagc acagtaccgg ctacgccgcc gagatcaaca tattctccgg
     2521 cacggaacgg gcggaacatg acaagtaaca cgccagcggc caggttagaa agtgacccct
     2581 gcaaagccag accaacagct aagcctgcgg caccgagtac agcaatgact gacgctgttt
     2641 gtacacccac gcgtcccagg gcagcgatta gcgtaaaggc gataataccg taacggacta
     2701 atgcagaaag aaaatcagca acagtggcat cgattttacg ggagatcatc aggcgattca
     2761 ccgcgttgga aatcatccgc gcgataatca aaccaacgat gatgatcgcg agtgccgcca
     2821 cgatgtttac tgcataactt agcagcagcg cctggttagc taccagccag cttcccgcgc
     2881 cgtttatgct atcgacaaca ttcaaatctt ccattcaata ttccttttca actgactcaa
     2941 aatggagaaa tacactccgc cgccttatga cgggcagtct gacaagggta aacaataaag
     3001 cataattttg ccaaatagat cacagatttc tcatctgaat caccttgctt ttggcgaaaa
     3061 aatccccgca gagcggggct cagacagata tcaatgtgcg ctttgtcatg ccggatgcgg
     3121 cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca atatattgca ggatacgtgt
     3181 aggcctgata agcgtagcgc atcaggcaat tttgcttttg gcgaaaaaaa gacccgcaga
     3241 gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg ttcagttcct
     3301 ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg
     3361 gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac tgcccctgca
     3421 gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat
     3481 cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt tcctgagcag
     3541 tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct
     3601 tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg ttacccggct
     3661 tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg ctgattttgc
     3721 tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca
     3781 tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag
     3841 tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg ttctcttgca
     3901 gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg gtagctgcga
     3961 agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt
     4021 ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg
     4081 caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac
     4141 cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag
     4201 cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg
     4261 cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc
     4321 caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat
     4381 actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
     4441 tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag
     4501 tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc ctgccctgtg
     4561 attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac tttaccttcc
     4621 acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc agcaatgccg
     4681 aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc ttcgctgtct
     4741 gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc gaacacgcca
     4801 accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag ttcctgagcg
     4861 gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac agatttcagg
     4921 gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg aaccgggatg
     4981 ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta cagggattta
     5041 cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc aacaatcagc
     5101 tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac tttagaacca
     5161 ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag cgcgtccagt
     5221 tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat accgtgagta
     5281 gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc acacagtgca
     5341 gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa gcgaacgttt
     5401 tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc tttaaccaga
     5461 cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag agagaattct
     5521 tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac tttcgcgcct
     5581 tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc gctggttact
     5641 ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt cccagcaaga
     5701 tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc taaaagtttt
     5761 gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg tcgtgtcgag
     5821 cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga tcaggtgtgc
     5881 gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt ggttaaaatc
     5941 tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc
     6001 tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa
     6061 atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa agcgatcgtt
     6121 aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga
     6181 ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc
     6241 gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa
     6301 caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg
     6361 ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga gatcgttact
     6421 gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt cgccatgctc
     6481 gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg
     6541 gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa caaaaagttg
     6601 atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa
     6661 atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcccg
     6721 gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat
     6781 cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag
     6841 agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac tgattggtta
     6901 attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa
     6961 aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt ttcagccccg
     7021 cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt
     7081 tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc
     7141 gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttccc
     7201 ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc cagctacacg
     7261 cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc ggtagcctga
     7321 ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac ggtacattcg
     7381 aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat ctctgcgttt
     7441 ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca
     7501 cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa ccttgcgcgc
     7561 tttctggtta cgacgagttg catatttccc ccgttaaata actactttca aagaggtttt
     7621 cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat atctatcgcc
     7681 atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt tctcgactcc
     7741 atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct gaccggctta
     7801 ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt tgcacccgtc
     7861 gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt tcgcaccctg
     7921 ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg
     7981 ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt cgttgcctgg
     8041 atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacagtgat aggcgcgaac
     8101 ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca gctgccgatt
     8161 cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc atttacctca
     8221 ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca gaccattttg
     8281 ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac gcggcggcag
     8341 gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct ggtacacggt
     8401 ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg gacgcatgct
     8461 attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg gatgcagtac
     8521 gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc aggcgtcgct
     8581 tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt agcgattatt
     8641 catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca gcgtttacgt
     8701 gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct ggctgtccgt
     8761 ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac gttgtgggca
     8821 ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct gatgctaaca
     8881 gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg acacactcaa
     8941 cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc gccatctttg
     9001 gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg tatccggact
     9061 ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt gggcatctcc
     9121 cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat ttgcagctct
     9181 ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg
     9241 ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag tgccagccgt
     9301 tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg gtcatcgcct
     9361 gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt cgcctgacgc
     9421 cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa cgtggttcgc
     9481 tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag caagcgtggc
     9541 aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat cttatcgctg
     9601 gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac aaggtgaatg
     9661 gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag taatggcagg
     9721 tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga aaaacctgaa
     9781 aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa accaactttt
     9841 tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga
     9901 tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag acactgccaa
     9961 ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg cctgcctgga
    10021 agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg acgaaaattg
    10081 gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga gcgtggtagc
    10141 aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc gactggaaga
    10201 tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt catcattaag
    10261 gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc gtcggtacgg
    10321 tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg ccccaccaga
    10381 cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca aaattgcgca
    10441 agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat gacgagtggt
    10501 gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta ctgcggccac
    10561 gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac gtcaaatgtc
    10621 tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt gtaatggtga
    10681 aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg cacgagatat
    10741 tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag aaaaaccacc
    10801 gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa cggtttatgc
    10861 acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt caccgttaat
    10921 tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc agcgccgttt
    10981 tgaccattcc gctaaaacgc gcc
//
LOCUS       DAZOXR010000146        10973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZOXR010000146 DAZOXR010000000
VERSION     DAZOXR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.149"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            67..828
                     /gene="adiY"
                     /locus_tag="ACHUNN_003246"
     CDS             67..828
                     /gene="adiY"
                     /locus_tag="ACHUNN_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0577547.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            965..2302
                     /gene="adiC"
                     /locus_tag="ACHUNN_003247"
     CDS             965..2302
                     /gene="adiC"
                     /locus_tag="ACHUNN_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0577548.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2406..4049
                     /gene="eptA"
                     /locus_tag="ACHUNN_003248"
     CDS             2406..4049
                     /gene="eptA"
                     /locus_tag="ACHUNN_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0577549.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4046..4714
                     /gene="basR"
                     /locus_tag="ACHUNN_003249"
     CDS             4046..4714
                     /gene="basR"
                     /locus_tag="ACHUNN_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0577550.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4715..5815
                     /gene="pmrB"
                     /locus_tag="ACHUNN_003250"
     CDS             4715..5815
                     /gene="pmrB"
                     /locus_tag="ACHUNN_003250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0577551.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5791..5880)
                     /gene="pmrR"
                     /locus_tag="ACHUNN_003251"
     CDS             complement(5791..5880)
                     /gene="pmrR"
                     /locus_tag="ACHUNN_003251"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0577552.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5992..7494)
                     /gene="proP"
                     /locus_tag="ACHUNN_003252"
     CDS             complement(5992..7494)
                     /gene="proP"
                     /locus_tag="ACHUNN_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0577553.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7758..8624)
                     /locus_tag="ACHUNN_003253"
     CDS             complement(7758..8624)
                     /locus_tag="ACHUNN_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0577554.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8621..10849)
                     /gene="crfC"
                     /locus_tag="ACHUNN_003254"
     CDS             complement(8621..10849)
                     /gene="crfC"
                     /locus_tag="ACHUNN_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0577555.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2740 a         2869 c         2691 g         2673 t
ORIGIN      
        1 accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc actcaggagt
       61 acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga aaaagatgtc
      121 tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat ggcgttatta
      181 aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt tgctcatatt
      241 agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc gcaaatacca
      301 gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc agacgtcttt
      361 cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa ggaacgaaca
      421 cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt cctttcatta
      481 atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat tgaaagcgat
      541 attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag cccaagtctg
      601 ttaaagaaaa agctgaaaag cgaaaacacc agttatagcc aaataatcac cacctgccgc
      661 atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca ggtatcacag
      721 tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt ctacggtatg
      781 acgccgctgc attatgttag tcagcacaga gaacgaactg tcgcctgatt tttaacctta
      841 acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa ttcaactatc
      901 gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc gcaggagttt
      961 aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca ccctgatggt
     1021 gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg cctccactgg
     1081 cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc tctcgatggt
     1141 atacgccaaa atgtcgttcc tcgatccaag tcctggtggt tcttacgctt acgcccgccg
     1201 ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg cctgctggat
     1261 cggcaatatc gctatggtgg tcattggcgt aggatattta agttacttct tcccgattct
     1321 gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct tcgtcctgct
     1381 gaacattgtc ggtccgaaaa tgatcacccg tgtgcaggcc gttgccaccg tgctggcgct
     1441 gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg aaacctatat
     1501 ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta cccttaacgt
     1561 tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg tggtgaaaaa
     1621 cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg ccgtttgcta
     1681 tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc gcgtttctgc
     1741 ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg ccattgtttc
     1801 cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc tggcgggtca
     1861 aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc gtgtaaataa
     1921 agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca tcttccagct
     1981 cagtagcatt tcgccaaacg caaccaaaga gttcggtctg gtatcttccg tgtcggtcat
     2041 ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg gccacggtca
     2101 ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc tctactgcat
     2161 ctgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca ccctgatggt
     2221 catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt atcccttaga
     2281 tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc caaccttaag
     2341 aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc gatggaaaca
     2401 ccgtgatgtt gaagcgccta ctaaaaagcc cctctttgaa tttactcgcc tggctattgt
     2461 tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag gtgttgcagg
     2521 cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg gtcgtcgctt
     2581 tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat cgaccactgg
     2641 cctgcctgtt tattctggtt ggcgcggctg cacaatattt cataatgact tacggcatcg
     2701 tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa agttatgcgc
     2761 tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct gcgctgattg
     2821 cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt ttccgtggag
     2881 ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt tataaagact
     2941 acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc tctaacagca
     3001 ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctaccg ctggtgcgaa
     3061 ttggtgaaga cgcccaccgc aacccgttaa tgcagaacga aaaacgtaaa aatttgacca
     3121 tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc tacccgcgtg
     3181 aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc gcatcttgcg
     3241 gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt gagcattaca
     3301 aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcaa gcgggcatca
     3361 acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc gtgcctcacc
     3421 agaacgtcac cgcgctgaat ctgcctggtc agtgcatcaa cggcgaatgc tatgacgaag
     3481 tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc gtgattgtct
     3541 tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg cagttcagga
     3601 aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag caactggtga
     3661 acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg attaatctgc
     3721 tgaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac cacggtgaat
     3781 cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc ccggatagcc
     3841 aaaaacaggt gccgatgctg ctgtggctgt cggaggatta tcaaaaacgg tatcaggttg
     3901 accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac aatttattct
     3961 ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggctgcg gatgatgttc
     4021 tgcaaacttg caggagagtg agtgaatgaa aattctgatt gttgaagacg atacgctgtt
     4081 attgcaggga ctgattctgg cggcgcaaac cgaaggctac acgtgcgatg gcgtaacaac
     4141 cgcgcggatg gcggaacaaa gccttgaggc cggtcattac agcctggtgg tactggattt
     4201 agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga aaaaatacac
     4261 cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg ccgggctgga
     4321 tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac acgcccgtat
     4381 ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg ttggcaatct
     4441 gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga ttctgacgcc
     4501 caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg tacatcggga
     4561 aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca ccctggaagt
     4621 gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg tgcgcggctt
     4681 tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt ttctgcgccg
     4741 accaatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt tggtgtttga
     4801 gctgatcagc atcttctggc tatggcatga aagtaccgag cagattcagc tgtttgagca
     4861 ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc gcgaggcggt
     4921 cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta tctgctatca
     4981 ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg aagcgcgcac
     5041 cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg aagcggtggt
     5101 ttcggcgttg aacgatctgg tcagtcgcct gacgagcacg ctggataacg aaaggttatt
     5161 taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt tgcatctgga
     5221 actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc ttgatcagat
     5281 gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat tttcttccgg
     5341 taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg acgaactcag
     5401 taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg ccgacatcac
     5461 cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag aaaacgccca
     5521 tcgttacagc ccacaaggca gcaacattat gattaagctg caagaagatg gcggagcggt
     5581 catggccgtt gaagatgaag gaccgggtat tgatgagagt aaatgcgggg agttgagtaa
     5641 agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa gtattgtcag
     5701 ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag agacttccgg
     5761 cacgcgggcc tgggtacggc tgaagaaaga tcagtacgta gcaaaccaga tataaagaaa
     5821 gctgctgacc accagcacac tgaacacggt agttaaactt tcataaacac ggtttttcat
     5881 tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag tgaaccttaa
     5941 gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag cttattcatc
     6001 aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg caatctcgtg
     6061 gtcaatatca tcgattttct gctcgatatt atcgtaatgc tcgacgagaa tttccttcgc
     6121 ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg ccgtctcttt
     6181 catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat aggcaggcat
     6241 catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca gaccggcaac
     6301 cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg tcgggaacat
     6361 cgctggcaag gtagaagcca taacgcccgt aaagcagtta aggatcaccg ccagcatcag
     6421 taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg ccgggatcgc
     6481 caacacaaac agggcaacac taccaagtag cacaaacgga cgacggccaa aacggtcact
     6541 cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga taataatcag
     6601 caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca tataggtcag
     6661 caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca acaggctgcg
     6721 ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca aaccttcgcg
     6781 gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt ccagcgcatg
     6841 gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa tacgccagcc
     6901 ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca cgcccgcacc
     6961 cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc cacgtttacg
     7021 gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccgc cgaccgagaa
     7081 accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa tcgtgtcgta
     7141 ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag tgatagcgag
     7201 gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagcccgc caagcggtcg
     7261 aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc tggggtcagc
     7321 ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac caaaatcgaa
     7381 ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt taccgtcatc
     7441 aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca gcatagcttt
     7501 cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg gccgcaaaag
     7561 gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg cttacaccct
     7621 ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt aatgaccttt
     7681 ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt ttaggactca
     7741 ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac cattgccccc
     7801 accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgactgtttg ccagcgttgg
     7861 gtcactgacc agcaaacggg ctgtttatct gccgtggacc actttcccag ccaacttaaa
     7921 aaatcagcat gctcccctcc cgaagaggca aaccactgat ggtaatagtg acgcgtggtc
     7981 ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt tctcgcgcaa atcacccagt
     8041 aacaacacca ccgcttgcgg gcttcccaga cggcaggcat cgccaaatgc accccagcgc
     8101 aactcctcca gaacgataaa acagcgccca ggcaacgacc aggcgttata cgccccagca
     8161 cgccagcggg taaaaatttg ctcgctctga tcacctgcct gcaccagtag gcctcgttgc
     8221 gttaacgcct tttccttcca gcgacaacgg gctgaaaacc actcatacaa ggcggcaatt
     8281 tgctggggga gctccgggaa agcagagtgc tggcggtgat ccattttttg cagcagcgcc
     8341 agatgctgcg cgatctgcgt cagcgccaga tgaccgggat ccagcatcga cgccagtttt
     8401 tccaccattg tcagtcgcag gctaagcggc gcatttaaca tgccgccgtt cgtccatgtc
     8461 tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt cacgaaactg ctgctgatgg
     8521 gcttgtggaa ggcgggcatc atcgccctgc gccagatcca ctaaaaagcg gggataaacc
     8581 gactccagca cgcgaccggg gccgtccagt aacgtcttgg tcaatatcgt tctgccgtga
     8641 aaagtgtttg aatatcatcg cgtaacagct gggcgtcggt gtaaatccag ccgtgagtca
     8701 tcacagtctg ctgcaattgc tgctgcatca gcctgaccac cgattcattt tgttgacgca
     8761 gagccaggct ttcgcgtaaa cgcgtctgta attccgtcaa acatgaagcg aactcagcgc
     8821 aaaaagtatt catgcctgcc gtaacagatt catcgacctg ctctgccaga actttagcca
     8881 tttgttggca ataaagatcg acttctgcgc ttaaagctcg ttgcaacaca ctgtaatcaa
     8941 ccgtctctgt cggggatttc tcatttcccc gtccccagtc gggctgattc aaccagcgcg
     9001 aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc gtccgttgca tcctggcgcg
     9061 aaatgacttc actgaacagc tggcgagtgt tgacgtgggg aactactccg tgaaaaacag
     9121 gaaaatgaac cccaggacga aaccctgact cgctcaattc cattttgact tgttgctcaa
     9181 tgggccgaat aacatcggtt aacacccggc aaagggtgga ttcaagctcg gcaaaacgca
     9241 gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa cagtgtctca cagcaggtac
     9301 gaatctcgct taacggctcc gaatcatcct gaaacaaggc ggctaactgc gcattcagcg
     9361 catcttgctg ttgacgcaga aagtggttgg cggaggtcag ggccagctcg atttcatgtt
     9421 taatctcgcc gctcacctgc gcctgattga gttgcaatag ctgcaaactt tcttcgacct
     9481 gatggatatt ttgccgcaat tgttcacaag cgacgtttaa cccgtgcgca cgaaaatcca
     9541 ggtattcccg cgcctgctgc gcgtaattca acagtttatg cgcagcagat cgcagagcat
     9601 acaacgaggc gttagcgtaa gcggcatgaa gcaacgcctg aattggctgg gcgaacagcg
     9661 aatcttccca caactgatcg gcagcatgac gaatatgttc gaggtccgcc agatcggcat
     9721 gacgccagcg cctgccgagc gcggcatggg caaaatcttc cacccagcgt tgttgctctg
     9781 gcgctggtaa cttaccgttg ttggctaact catggcgcgc tcgatttgcc aagtagcccc
     9841 acatcgacga caccggaaat atctgctgtg gcgtaataca gcctttcatc agcgtcccgg
     9901 aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg atcctgttga tcgaacttat
     9961 tgaccagcac atacagtggc accgattgcc ccaccgccaa aatcgcctca cggacctctt
    10021 catcggagat cgatttcagt tgcgtataat ccagcaccgc cagtaccgcc gaggcgcgtg
    10081 ccagctgctg gttaagcatt ttttgcagat gcggttgccc ggcttcattt ggcccggggg
    10141 tatccagtaa cgtcaactga ccgggataac tctccagccc cgccagatgg acaaactcca
    10201 cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga aaaaggaaaa tcgacgtcca
    10261 gcgccttcgc cagtcgcact aaatcattca aacttttcag acaatgaaaa ataggctggg
    10321 cacccagata atatttttcg aaagcgacgc cattttcgat ccgctgcata agcgcacgca
    10381 tatctttatc tatttccagc acatcggtca gatgcttaat atcgcaatca cgcaggcgct
    10441 gttgtaattg ttgaattaaa caatcgattg gcgcgacatg tgaaaaatgc agtaccggtt
    10501 ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc agtcattggg cgattacgat
    10561 taggcagaac ctccgtacca acaatggcat taatggtggt tgatttccct gctttcatag
    10621 taccgacaat cgccagcacc atttccagtc gggaaatttt actcaactca ttattcagca
    10681 tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa agccagttgt ggcgcgtcgt
    10741 ctccgggtac agagagaggc attttttcca gtaactgcaa ctgttggcga gaaagctgta
    10801 acaggcgttc agcctcctga cttaactcat acagggtctg tgtgtacata gaaaattctt
    10861 ccttaaagca aattttgtta cttttattta gccagattgt ttttgagttc tgtttgagtt
    10921 ctgtttttgg cttttataat tactgcaaga aataatttta tatttagtgt gtt
//
LOCUS       DAZOXR010000147        10930 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZOXR010000147 DAZOXR010000000
VERSION     DAZOXR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.150"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            47..319
                     /gene="rcnR"
                     /locus_tag="ACHUNN_003255"
                     /pseudo
     CDS             47..319
                     /gene="rcnR"
                     /locus_tag="ACHUNN_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      411..507
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            542..1330
                     /gene="thiM"
                     /locus_tag="ACHUNN_003256"
     CDS             542..1330
                     /gene="thiM"
                     /locus_tag="ACHUNN_003256"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0577556.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            1327..2127
                     /gene="thiD"
                     /locus_tag="ACHUNN_003257"
     CDS             1327..2127
                     /gene="thiD"
                     /locus_tag="ACHUNN_003257"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0577557.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            2192..3010
                     /locus_tag="ACHUNN_003258"
     CDS             2192..3010
                     /locus_tag="ACHUNN_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR0577558.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            3062..3808
                     /locus_tag="ACHUNN_003259"
     CDS             3062..3808
                     /locus_tag="ACHUNN_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0577559.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(3782..4747)
                     /locus_tag="ACHUNN_003260"
     CDS             complement(3782..4747)
                     /locus_tag="ACHUNN_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0577560.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4744..5748)
                     /locus_tag="ACHUNN_003261"
     CDS             complement(4744..5748)
                     /locus_tag="ACHUNN_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR0577561.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(5745..7022)
                     /locus_tag="ACHUNN_003262"
     CDS             complement(5745..7022)
                     /locus_tag="ACHUNN_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR0577562.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            7279..8331
                     /gene="fbaB"
                     /locus_tag="ACHUNN_003263"
     CDS             7279..8331
                     /gene="fbaB"
                     /locus_tag="ACHUNN_003263"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0577563.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            8650..9504
                     /gene="gatY"
                     /locus_tag="ACHUNN_003264"
     CDS             8650..9504
                     /gene="gatY"
                     /locus_tag="ACHUNN_003264"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0577564.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            9533..10790
                     /gene="gatZ"
                     /locus_tag="ACHUNN_003265"
                     /pseudo
     CDS             9533..10790
                     /gene="gatZ"
                     /locus_tag="ACHUNN_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            10800..>10930
                     /locus_tag="ACHUNN_003266"
     CDS             10800..>10930
                     /locus_tag="ACHUNN_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0577565.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         2775 a         2768 c         2854 g         2533 t
ORIGIN      
        1 taatagtgct atgatatttt catattcttg ttaaccaggt gttgccatgt ctcatacaat
       61 ccgtgataaa cagaaactga aagcgcgtgc cagtaagatt cagggctagg tcgtggcgct
      121 caagaaaatg ctcgacgagc cgcatgaatg cgctgcagtt ttacaacaga ttactgctat
      181 ccgtggcgcg gtaaacggtc tgatgcggga agtgattaaa ggtcatctga cggaacacat
      241 cgttcaccag ggggatgagc taaaacgtga agaagatctg gatgtcgttc tgaaggtgct
      301 ggattcatat atcaaataat ttattaacgc gattgtaaaa ctgccgtttt tcctcgttta
      361 caacgcgtgc gctggacatt accatcctcc tctgcgattt atcatcgcaa ccaaacgact
      421 cggggtgccc ttctgcgtga aggctgagaa atacccgtat cacctgatct ggataatgcc
      481 agcgtaggga agtcacggac caccaggtca ttgcttcttc acgttatggc aggagcaaac
      541 tatgcaagtc gacctgctgg gttcagcgca atctgcgcac gcgttacacc tttttcacca
      601 acattcccct cttgtgcact gcatgaccaa tgatgtggtg caaaccttta ccgctaatac
      661 cttgctggcg ctcggtgcat cgccagcgat ggttatcgaa accgaagagg ccagtcagtt
      721 tgcggctatc gccagtgcct tgttgattaa cgttggcaca ctgacgcagc cacgcgctca
      781 ggcgatgcgt gctgccgttg agcaagcaaa aagctctcaa acaccctgga cgcttgatcc
      841 agtagcggtt ggtgcgctcg attatcgccg ccatttttgt catgaacttt tatcttttaa
      901 accggcagcg atacgtggta atgcttcgga aatcatggca ttagctggca ttgctaatgg
      961 tggacgggga gtggatacca ctgacgccgc agctaacgcg atacccgctg cacaaacact
     1021 ggcacgggaa actggcgcaa tcgtcgtggt cactggcgag gtggattatg ttaccgatgg
     1081 ccatcgcgcc gttggcattc acggtggcga tccgttaatg accaaagtgg taggaactgg
     1141 ctgtgcatta tcggcggttg tcgctgcctg ctgtgcgtta ccaggcgata cgctggaaaa
     1201 tgtcgcatct gcctgtcact ggatgaaaca agccggagag cgcgcagtcg ccagaagcga
     1261 ggggctaggc agttttgttc cacatttcct tgatgcgctc tggcaattga cgcaggaggt
     1321 gcaggcatga aacgaattaa cgctctgacg attgccggta ctgatccgag tggtggtgcg
     1381 gggattcagg ccgatcttaa aaccttctcg gcacttggcg cttatggttg ctcagttatt
     1441 actgcactgg tggcgcaaaa tacccgtggc gtacagtcgg tgtatcgcat tgagcctgat
     1501 tttgtcgccg cccagctcga ttcggtgttc agcgatgtgc gaatcgatac cactaaaatc
     1561 ggtatgttgg cggaaaccga tattgttgaa gcggtggcag aacggttgca acgttatcag
     1621 atccaaaacg tggtactcga caccgttatg ctggcaaaaa gcggcgaccc actgctttca
     1681 ccttcggcag ttgccacgct acgcagtcga ttattgccac aggtttcatt aataacgcca
     1741 aacttgcccg aagcagccgc cttgctcgac gcgccacacg cgcgcaccga acaggaaatg
     1801 ctggaacaag ggcgatcgct gttggcgatg ggctgtggcg cagtgctaat gaaaggtggt
     1861 catctggatg atgagcaaag cccggactgg ctgtttaccc gcgagggtga acaacggttt
     1921 accgcaccgc gcattatgac caaaaacacc cacggtactg gttgtacact ctctgcggcg
     1981 ttggctgcac tacgcccgcg ccatacaaac tgggctgaca ccgtacagga ggcaaaaagc
     2041 tggctttcat cggcgttagc ccaggccgac acgctggaag ttggtcacgg tattggtccg
     2101 gttcaccact tccacgcctg gtggtgagca ccgtactata gctgctttgg ttattgtcag
     2161 cctttggcac tgacattcag ggatgaaacc aatgcagcta agaattacca gtcgaaaaaa
     2221 actcaccgct ctattatgcg ctcttgggct aatttccatc gttgcaattt atccgcgtca
     2281 gacggtgaat tttttctatt cgacagcagt tcagattaca gactacatcc acttctacgg
     2341 ttatcgcccg gttaaatctt tcgctattcg tattcctgcc agttacacca ttcacggaat
     2401 agatgtttcg cgctggcagg agcggatcga ctggcagcgt gtggcaaaaa tgcgcgataa
     2461 cggtattcgc ttacaatttg cctttattaa ggcgacggaa ggcgaaaagc tggtggaccc
     2521 ctatttttcg cgtaactggc aactaagccg cgaaaatggc ttgctgcgcg gggcatatca
     2581 ttatttctcc ccgtcggtag cagcaccggt tcaggtgcga ttatttctgc aaacggtaga
     2641 tttctcacaa ggcgatttcc ctgcggtact ggacgtagaa gagcgaggaa aattatcggc
     2701 aaaagaatta cgcaagaggg taagtcagtg gctaaaaatg gtcgaaaaaa gtacggggaa
     2761 aaagccgatt atttactcag gagccgtttt ttatcacacg aatctggcgg gctatttcaa
     2821 tgaatatccg tggtgggtgg ctcactacta tcaacgtcgt ccggacaatg acggcatggc
     2881 ctggcgcttc tggcagcatt ccgaccgtgg acaggtagat ggcatcaatg gtccggtgga
     2941 ttttaatgtg tttaatggca cggtggagga gttgcaggga ttcgttgatg ggattaaaga
     3001 aacgccttaa ataagaagca gtgatatact cccaccagac tccctggcag gaaaaagaca
     3061 aatggaacaa gcgcataccc agctgattgc ccaactgaac gaacgtattt tagcggcgga
     3121 taacacgccg ctttatatta agtttgccga aacggtaaaa aatgccgtgc gcagcggggt
     3181 gctggagcat ggcaatattt tgcccggtga gcgtgattta agtcagttaa ccggcgtttc
     3241 gcgcattacg gtgcgcaagg cgatgcaggc gctggaagaa gagggcgtgg tgacgcgttc
     3301 gcgcggttac ggcacgcaga tcaacaacat cttcgaatac tcgttgaaag aagcgcgagg
     3361 tttttctcag caggtggtgt tgcgcgggaa aaagcccgat acgctatggg ttaacaagcg
     3421 tgtcgtgaaa tgtcccgaag aagtcgcgca gcagctggcg gtcgaagcag gaagtgatgt
     3481 ctttttgctt aagcgtattc gctatgtcga tgaagaagcg gtatcgattg aggaatcgtg
     3541 ggttccggcg catttaattc atgatgttga tgccatcggg atttcacttt atgactattt
     3601 ccgcagccag cacatttacc cacaacgtac gcgttcccgc gttagcgccc ggatgccgga
     3661 tgccgagttt cagtcacata ttcagttaga tagcaaaata ccggtgctgg tgatcaagca
     3721 agtggcgctt gaccaacagc aacggcctat tgagtacagc attagccact gtcgcagcga
     3781 tctatacgtt tttgtgtgcg aggagtagtt cctcgcgcgt tggcgcacaa tcaccgcccc
     3841 gatgcccgac aacccacgac gccactgcat tgcccagtaa tacggcatcc gccagtggca
     3901 gaccagaggc cagcccggca agtacgccac ctgcatgact gtcgcccgcc ccaatggtgt
     3961 ctacaacttg cgttggaaat gccggaatgc agccagaagc gtcgttgctg aaataccatg
     4021 cgccttcttt atcgagacga acgatcaacg gcgcggcaaa tttctcctgc cattgctcgc
     4081 caagtgttgt tatctctgcg gataaagcaa aacgttcggc ggcaatctca gcctcttgac
     4141 gattgagcga cactaaaggt cgacaggcca tgatccgcgc cagtaatgca tccgggatat
     4201 cgccaatacg tgggccaaaa tcgataaacg gcgtcacgtc ttgcagctct tccagccatt
     4261 ccactaacaa ttcgccgcag ggcgaggcca gttgataacc ggaaaaatag agcaggctgc
     4321 caggcgcaac ggttaatcgc gccagccact ggcgattcca ctgattttcc acaccgctga
     4381 atgacataaa agtgcgttcg ccatccggct caaccagcgc cagacaccag ccgttatcac
     4441 cttcggcgtt atcgatcagg ctgattaagc cctcttttgc catccggttg cgaataatct
     4501 ccgcccacac gccctgaccg agcggcaagg cattacccgc ttcgatgccg aggcgcttta
     4561 acgccacggc aatattcagt gcgcagccgc caacgttaac gctctgctgt ttcagttcga
     4621 tatcgcaccc acgccagggg agggcataag cgtcggcgat cacgtcaatg accgccgcgc
     4681 cgacgaccat caccggctga cgcgtggtta attcaggcag cagcgtgtgt aatcgagcgc
     4741 cgctcatacc gcctcccgtt gttgacgata tttcgccagc gctgtggcat agcggttgaa
     4801 atcaagctga tttaccgcat ccagttccgc ctttaacgca ggatcgatag cgttaacgcc
     4861 atgcaacgcg ccgcaaattg ccgtcgccat agcaccgatg gtgtctgtgt cgccgccaag
     4921 gttagcgcac aggacggcgc agcgattcgg gtcggtttgt gctagttcaa ccagcgcaat
     4981 ggcgcacgga acggactcaa tagtgctggt acctgcgcca acgacctggt aaagctgttc
     5041 gctggcggat tcggtgccgt cggcattgcg cacaattttc agcgcaatct ccagccgcgc
     5101 cgccagtgag gcgctgaacg tagtgatgcg tttttgctgt gcatgtcgcg caattgaagg
     5161 cagggaatca acaatcgctg accagctttc tccgtcaatg gcacgagaaa tcgcccatgc
     5221 gatgactacc gcgcctgcaa ccgccagatc ggatttatgg gtcgggctgg acgccagcgc
     5281 tacatcatca ataaaggaat caacatcacg cgccggaagc aaacaaccta atggcgagac
     5341 gcgcatcgct gcgccgttgg tcacgccgtt gttttccagt tcagcaacgg gtttaccgtc
     5401 gcgaatggcg ttaagcgcaa tcttcgaggt cggacctagt acgtttttgt taaaggcgtc
     5461 gaaacgcagc gcccagtcga gaatattacg cccaatcaga tccggatcga tcttgccttc
     5521 acgttccagt aacgcatccg ccagacacag cgccatcgag gtatcgtcgg tgaattcggc
     5581 gcggttaaaa taacaggccg cgttattctc ctttggtcca ggaagaaaac ggtcaatcca
     5641 gccaaagtgt gctttaacgc ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc
     5701 ccctaacgcc tgcccataaa gagcaccgag aatacgttct gttttcattt aacttcccct
     5761 tgtgtcaacg caatatcgcg atcatcgacc ttgatagccg taatttcgtt gtcggattcg
     5821 cggaaaaaaa tcatgaacag cacggcgata atggcaatca tcaccgcgcc gaaagtccac
     5881 atcccggacc agttgaaagt cagtccgttt accggttcct gataagcgaa catcttttcc
     5941 atcatcacac cgccaagacg atagccgagc aaactgccga agccctggca gcagagcgtg
     6001 atcagcccct gcgcagcggt acgcatatgc acgggggctt ttttatcgac atagatgtaa
     6061 gcggtaacgt agtaaaaatc gtaacttacg ccgtgaagca aaataccgag gaacagtaac
     6121 gcgtaggtga aatattcatc cgtactaccg taaataaaga agccatagcg gatcgcagcg
     6181 gtgaccagac caagcaataa tacctttttg ataccaaagc gtttagtgaa aaacggcaat
     6241 gccagcataa agaagatttc agagaactgg ccgagcgtca tccagccggt ggcgtttttc
     6301 atgccaactt cggtcagata accgttggca aagatgtaat agaacgccag tggcatcgca
     6361 aacaggaatg aacagaagaa aaagacgagg aagtttttat cgcgcagcag gatcagcgca
     6421 tccaggccga gcatgacttt aatatccatt ttgccggtgc tttttggtgg cgtgtcgggc
     6481 aggaaaaacg caaacacacc gagcagagca gaacttccgg cggtaatcag cagcgggatg
     6541 ttagtcggtg agatatcggc ataccccagt atttgcggca agaaaccaca tgccagaccg
     6601 gaggcaatcc agccgatagt gcccatcaca cgaatgcgcg ggaaatcacg ctccacatcc
     6661 ggcacgttgg caaaagcgat gctgttagtc agcgcaatgg tcggcatata ggttagagag
     6721 taagccagca gtaacggaaa gaacccggca aaagtggtct gttgcgcagc gaaatacatc
     6781 agcagcgcgc ctgcgaacat caataccgcc agcacttttt gcgccgagaa aaagcggtca
     6841 gtgatggagc caaccagaat tggcgacagg atcgccgcaa tggcggtaca ggcatacgac
     6901 cagccaattt ctccggcact aaaaccgctt ttacttaacc acaaccacaa tggcacaaac
     6961 cacgcgcccc agataaacca ttcaacaaac atcatgaacg acagctttgc tgttgttttc
     7021 atcttttaat tccttcatga caggtaatgt aaggtacgcg atgacaatac cacttaacaa
     7081 taccttttaa atacctttgc ggtgaaaact tgaccgcgct aacatttttt ctgatgaatc
     7141 gagccaacag aaaacgctga aaaaacatcc aaaagatgga aaaactcgcc cgtaacgacc
     7201 aggattatga ttgcccgcaa aaggcgggcc aggacaatcc atagccgata tccaatcgga
     7261 atttacggga gcatagtaat gacagatatt gcgcagttgc ttggcaaaga cgccgacaac
     7321 cttttacagc accgttgtat gacaattcct tctgaccagc tttatctccc cggacatgac
     7381 tacgtagacc gcgtaatgat tgacaataat cgcccgccag cggtgttacg taatatgcag
     7441 acgttgtaca acaccgggcg tctggctggc acaggatatc tttctattct gccggttgac
     7501 cagggcgttg agcactctgc cggagcttca tttgctgcta acccgctcta ctttgacccg
     7561 aaaaacattg ttgaactggc gatcgaagcg ggctgtaact gtgtggcgtc aacttacggc
     7621 gtgttggcgt cggtatcgcg gcgctatgcg catcgcattc catttctcgt caaacttaat
     7681 cacaacgaga cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc cagcgtggag
     7741 caggccttca acatgggcgc ggtggcggtt ggtacgacta tctattttgg ctcggaagag
     7801 tcacgtcgcc agattgaaga aatttctgcg gcctttgaac gcgcgcacga gctgggcatg
     7861 gtgaccgtgc tgtgggccta tttgcgaaac tccgccttta agaaagatgg cgttgattac
     7921 catgtttccg ccgacctgac cggtcaggca aaccatctgg cggcaaccat cggtgcagat
     7981 atcgtcaaac aaaaaatggc ggaaaataac ggcggctata aagcaattaa ttacggttac
     8041 accgacgatc gtgtttatag caaattgacc agcgaaaacc cgattgatct ggtgcgttat
     8101 cagttagcta actgctatat gggccgggct gggttgataa actccggcgg tgctgcgggc
     8161 ggtgaaactg acctcagtga tgcagtgcgt actgcggtta tcaacaaacg cgctggcgga
     8221 atggggctga ttcttggacg taaagcgttc aagaaatcga tggctgacgg cgtgaaactg
     8281 attaacgccg tgcaggacgt ttatctcgat agcaaaatta ctattgcctg atacgcctca
     8341 tacgaacagc accaccccgt aggacggata aggcgtttac actgcatccg gcaacctatg
     8401 cctgatgcga cgcggaagcg tcttatcatg cctacatagc actgccacgt atgtcggaaa
     8461 aggcgtttac accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg
     8521 gaggccgttt ttttgccttt cactcctcga acagttttca tattgtcgtt ttgtgatcgt
     8581 tatctcgaca tttaaaaaca aatcatttca ttatattttg aaatcgaaaa caagcaacag
     8641 gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca gcgcggcggt
     8701 tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt ggtggaaacc
     8761 gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt cacttatgct
     8821 ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca ccatccactg
     8881 gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt tcgttctggc
     8941 gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat ttcacgggtc
     9001 aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaagcgga gctggggcaa
     9061 cttggcggcc aggaagatga tgtgcaagtc aatgaagccg atgtttttta taccaacccc
     9121 gctcaggcgc gtgaatttgc cgaggcaacc ggaattgatt ccctggcggt cgccatcggc
     9181 acggctcatg ggatgtatgc cagcgcaccg gcgcttgatt tttctcgact ggagaacatt
     9241 cgccagtggg tgaacttacc gctggtgctg catggcgcgt cagggttatc gactaaggat
     9301 attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga gctgaaaaat
     9361 gccttctcgc aggcgttaaa aaattacctg accgagcacc ctgaagcgac cgatccccgg
     9421 gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt gattgccgat
     9481 tgtggctgcg agggcagggc ataacgcacc tgccatttaa caaggaaaaa acatgaaaac
     9541 gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct gttctgccca
     9601 tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca aagtgctgat
     9661 tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga caccggcaga
     9721 ctttcgcgaa tttgttttta cgattgccga taaagtcgga tttgcacgcg aacgcattat
     9781 tctcggcggc gatcatctgg ggccaaactg ctggcagcaa gaaaatgcgg atgcggcgat
     9841 ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct ggcttcagta aaattcatct
     9901 tgatgcgtca atgtcctgcg cgggggatcc cataccgtta gcaccagaaa cggttgcgga
     9961 acgagctgct ttgctttgct gcggaaagtg tggcgacaga ttgccagcgt gagcaactga
    10021 gctatgtcat tggcactgaa gttccggttc cgggcggtga ggccagcgcc attcagtcag
    10081 tacacatcac ccatgttgaa gatgccgcca atactttacg tacgcatcaa aaggccttta
    10141 ttgcccgtgg gctgacagag gcgttaacac gcgtgattgc catcgtggtg cagccgggcg
    10201 tggaatttga tcacagcaat attatccatt atcagccgca ggaagcgcag ccgctggcgc
    10261 aatggataga aaacacccga atggtttatg aagcacattc taccgattac cagacccgga
    10321 cggcttattg ggaattagtc cgcgatcact ttgcaatatt gaaagtcggt cccgcattaa
    10381 cctttgcttt acgcgaggcg atatttgcac tggcacaaat tgagcaggaa cttatcgccc
    10441 ctgaaaatcg cagcggttgc ctggcggtaa ttgaagaagt gatgctcgac gaaccgcaat
    10501 actggaaaaa atattatcgt acgggtttta acgattcatt actggatatt cgttacagcc
    10561 tgtcggatcg tattcgttat tactggccgc atagtcggat taaaaatagc gtcgaaacga
    10621 tgatggtgaa tcttgaaggc gtggacatcc cactgggcat gattagtcag tatcttccca
    10681 aacaatttga acgcattcag tccggggaat tatcagcaat accgcatcag ctgattatgg
    10741 ataaaattta tgatgttttg cgcgcctatc gctacggctg tgcggaataa ggacggtata
    10801 tgactaacct gtttgttcgt agcggaattt cttttgtcga tcgtagcgaa gttttaaccc
    10861 atatcggtaa tgagatgctc gccaaaggtg tggttcatga tacctggcca caggcattaa
    10921 ttgccagaga
//
LOCUS       DAZOXR010000148        10718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZOXR010000148 DAZOXR010000000
VERSION     DAZOXR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.151"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..1093
                     /gene="dacC"
                     /locus_tag="ACHUNN_003267"
                     /pseudo
     CDS             1..1093
                     /gene="dacC"
                     /locus_tag="ACHUNN_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="ACHUNN_003268"
     CDS             complement(1140..1898)
                     /gene="deoR"
                     /locus_tag="ACHUNN_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0577566.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="ACHUNN_003269"
     CDS             complement(1956..2552)
                     /gene="ybjG"
                     /locus_tag="ACHUNN_003269"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0577567.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2837..4064
                     /gene="mdfA"
                     /locus_tag="ACHUNN_003270"
                     /pseudo
     CDS             2837..4064
                     /gene="mdfA"
                     /locus_tag="ACHUNN_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4105..4389)
                     /gene="ybjH"
                     /locus_tag="ACHUNN_003271"
     CDS             complement(4105..4389)
                     /gene="ybjH"
                     /locus_tag="ACHUNN_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0577568.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="ACHUNN_003272"
     CDS             complement(4475..5290)
                     /gene="ybjI"
                     /locus_tag="ACHUNN_003272"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0577569.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5290..6498)
                     /locus_tag="ACHUNN_003273"
     CDS             complement(5290..6498)
                     /locus_tag="ACHUNN_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0577570.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6582..7118
                     /gene="rcdA"
                     /locus_tag="ACHUNN_003274"
     CDS             6582..7118
                     /gene="rcdA"
                     /locus_tag="ACHUNN_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0577571.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7293..8978)
                     /locus_tag="ACHUNN_003275"
     CDS             complement(7293..8978)
                     /locus_tag="ACHUNN_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0577572.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9248..9625
                     /locus_tag="ACHUNN_003276"
     CDS             9248..9625
                     /locus_tag="ACHUNN_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0577573.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="ACHUNN_003277"
     CDS             complement(9655..9912)
                     /gene="grxA"
                     /locus_tag="ACHUNN_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0577574.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10072..10359
                     /locus_tag="ACHUNN_003278"
     CDS             10072..10359
                     /locus_tag="ACHUNN_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0577575.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10343..>10718
                     /gene="nfsA"
                     /locus_tag="ACHUNN_003279"
     CDS             10343..>10718
                     /gene="nfsA"
                     /locus_tag="ACHUNN_003279"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0577576.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2650 c         2715 g         2588 t
ORIGIN      
        1 gatgcgcgtg catggatttt aatggattac gccagcggta aagtgctggc agaaggcaac
       61 gcggatgaga aactggatcc cgcgagcctg actaaaatca tgaccagcta tgtggttggg
      121 caggcgctta aggccgataa gattaaactc accgatatgg tgacggtcgg taaagatgcc
      181 tgggcgacgg gaaatccggc actgcgtggt tcatcggtaa tgttcctcaa accgggcgat
      241 caggtttcgg tggcagactt gaacaaaggt gtgattatcc agtccggtaa tgacgcctgt
      301 attgcgctgg ccgattacgt tgccgggagc caggagtcat ttattggttt gatgaatggt
      361 tatgccaaaa aaactgggtc tgaccaacac taccttccag acggtgcacg gtctggatgc
      421 gccggggcag ttcagtaccg cgcgcgatat ggcattgctg ggtaaagcat tgatccacga
      481 tgtgccggaa gagtacgcca ttcataaaga gaaagagttc accttcaaca aaattcgtca
      541 gcctaaccgt aaccgtctgc tgtggagcag caatctgaat gttgatggca tgaagacagg
      601 aaccacggca ggtgcgggat ataatctggt tgcttcggct acccagggtg atatgcgttt
      661 aatctccgta gtgctggggg cgaaaactga ccgtatccgt tttaatgagt ctgagaaatt
      721 attgacctgg ggtttccgct tctttgaaac tgtgacgcca attaaacctg atgccacctt
      781 tgtgactcag cgcgtctggt ttggtgataa gagcgaagtg aatctcgggg caggcgaagc
      841 gggctccgtg accataccgc gtgggcagct gaaaaacctg aaggcgagct acacgttaac
      901 ggaaccgcag cttaccgcac cgctgaaaaa aggtcaggtt gtcgggacca ttgatttcca
      961 gcttaacggt aaatccattg agcagcgtcc gctgatcgtg atggaaaatg tggaagaggg
     1021 cggattcttt ggtcggatgt gggatttcgt gatgatgaaa ttccatcagt ggttcggcag
     1081 ctggttctct taatcttctg ataaccggat ggcgcgaaac gtcatccggt tatacgtcat
     1141 taatacatca acttaatacg gtgcgtctgc gcgtacttca catactcatc ttccgggcaa
     1201 caatcactca ccacaatatc aaagcgtttc aggtcaccca tgcgcgccgg acgcaccttg
     1261 ccaaatttac tgtggtcgac aaccagcaca tgcttttgcg ccatcgacat ggcccagtgt
     1321 tttaccggca actcttcaag attaaaacag gtagcgcctt tactgacatg cacgcccgcc
     1381 gcggaataaa aagcgatatc cgggcaaaaa ttattcaacg tttgctgaaa atcgatgggg
     1441 ttgaaaatgg cgttgctggc gtgaaattca ccaccgcaca gaaatgcgcg gcaatggggt
     1501 ttctctttca gcgccagaaa ggtatttagc gaataacaaa cggcggtaaa agggatttca
     1561 ttatcaatcg cttcaataat ccacggcgtg gtggtgccac agtcaaaaaa gagggtctga
     1621 tcgggttcta ccagtgtcgc agccagtttt gccgcccggc gtttttcttc caccaggcgg
     1681 gatttttgat cgcttaacag gtaatggctg gcactgcgcg gttccagaac aatatagccg
     1741 ccgagcaaaa cgacgggcgc actgtggttg ttcagatcgc gacgaatcgt catctccgaa
     1801 accccaagca gggcggcggc gtctttaaga tgtaacttat cgctgcgttt taattcttgc
     1861 agcagctgcc cgatacgctc ttcgcgacgt gtttccataa tccctctgaa tagttattga
     1921 agcgagccgc tcaatactac actttttagc agagatcagt cacgcaccca gcctttgcgg
     1981 atcggtaatg caaaacagac gcgataccac gattgcagac gttgatagag tttatgcccc
     2041 atcgcttgcc agataatctg ggcactaagg cagccaatca tacctgccag caatccaccg
     2101 agcatatcca gcggccagtg gacgccaaga taaacgcgcg accaggcaat gacgacggcc
     2161 agaaccatta aaagtgagcc ggaccacagg cgatgccagc ataaaaatgc cagtgcaaag
     2221 gtgaaaatca ccgtaccgtg atcgctcggg aatgagtcat ccgccgcatg atgcaggaag
     2281 ttatagccga tattttcgac aaagggtcgg tcgtgcggaa aaagatgtcc catcgtccag
     2341 gacacaaaca ggctgaccgc cagcgcgata gcgattttta tcaccagttg ccgttgtgct
     2401 gtaagccccc acaaccaaag taccgcggcc agcaacggca ccacggtaat caaatcttta
     2461 gcaataaaaa tcgccaacga gatcatccac ggagccgagt ctggcgtcgc gttaataaga
     2521 gagaatagag agagattcaa attttccagc ataacttccc gacgcaaagt gattaaaagg
     2581 gagccaatac aggcaagtcg ttgagaataa agtgcaggtt aaactgggta aagcggcatc
     2641 gtcttatttc cctcaagcgg cctgtttacg gtgggtgatt gtaacgggca taggttaaat
     2701 aaaacttaaa gaaagcgtag ctatactcgc aataatgtaa gaatgtgctt aaccgtggtt
     2761 tcagctacaa aattcgcttt ctcgttagct gcgcttttat taaactctgc gcgattatta
     2821 ttggcgaaga aattgcatgc aaaataaatt agctttcggt gccaggcttg gacgtcaggc
     2881 gttacttttc cctctctgtc tggtgcttta cgaattttca acctatatcg gcaacgatat
     2941 gatccaaccc ggtatgttgg ccgtggtgga acaatatcag gcgggcattg attgggttcc
     3001 tacttcgatg accgcgtatc tggcgggcgg gatgttttta caatggctcc tggggccgct
     3061 gtcggatcgt attggtcgcc gtccggtgat gctggcgggt gtggtgtggt ttatcgtcac
     3121 ctgtctggca atattactgg cgcaaaacat tgaacaattc accctgttgc gcttcttgca
     3181 gggcataagc ctctgtttca ttggcgctgt gggatacgcc gcaattcagg aatccttcga
     3241 agaggcggtt tgtatcaaga tcaccgcgct gatggcgaac gtggcgctga ttgctccgct
     3301 acttggtccg ctggtgggcg cggcgtggat ccatgtgctg ccctgggagg ggatgtttgt
     3361 tttgtttgcc gcattggcag cgatctcctt tttcggtctg caacgagcta tgcctgaaac
     3421 cgccacgcgt ataggcgaga aactgtcact gaaagaactc ggtcgtgact ataagctggt
     3481 gctgaagaac ggccgctttg tggcgggggc gctggcgctg ggattcgtta gcctgccatt
     3541 gctggcgtgg atcgcccagt cgccgattat catcattacc ggggagcagt tgagcagcta
     3601 tgaatatggc ttgctgcaag tgcctatttt cggggcgtta attgcgggta acttgctgtt
     3661 agcgcgtctg acctcgcgcc gcaccgtacg tgataattat gggcggctgg ccgattatga
     3721 ttggtctgtt ggtcgctgct gcggcaacgg ttatctcatc gcacgcgtat ttatggatga
     3781 ctgccgggtt aagtatttat gctttcggta ttggtctggc gaatgcggga ctggtgcgat
     3841 taaccctgtt tgccagcgat atgagtaaag gtacggtttc tgcggcgatg ggaatgctgc
     3901 aaatgctgat ctttaccgtt ggtattgaaa tcagcaaaca tgcctggctg aacgggggca
     3961 acggacagtt taatctcttc aaccttgtca acggaatttt gtggctgtcg ctgatggtta
     4021 tctttttaaa agataaacag atgggaaatt ctcacgaagg gtaaaaaaat gcctgactgc
     4081 tttgtgcgat caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac
     4141 catcccttca cctacgctgg caagatggcg agcataagga tgcgggcggt aagccggagc
     4201 tggcgcagga gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac
     4261 gctgactgaa tcaggaacta caaccacttt gtatccactc ggcacatcga cggtgacact
     4321 ttgcgccatc gccacaccag taaagcccat taaaagcgcg cccaacagta gacaattttt
     4381 cataatcatt tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca
     4441 ttcaggtatt gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt
     4501 taagcacttt atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt
     4561 ttgccgctgc gacgaccgcg ctgccggcat tttccattgc aaaactaaag cctgcctgac
     4621 gcagcatctc aatatcgtta ccgccatcgc caaagaccac cacttcgctg tcgtctattc
     4681 cccataattt ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat
     4741 cgatgctgcc gttgccggtg tggactggca ccataatatc gccgatggcc tcatgtaatg
     4801 ctttttgtac ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaaaatat
     4861 cttctaagtt gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tctgccaccg
     4921 ttttcatggc atcgtcatat tttttgagcg tataggcgct attttttccg caggcaataa
     4981 tttccacttc cgggcgcgtc agcaaatgtt ccacgacagt agtaaaggca tccttcgaca
     5041 gctcgccatt aaaaacatct ttgccttcac tcactaccca gccgccgttt tcagccacaa
     5101 aggcaatttc attagcaatt tcagggaaga aagagagcaa ctgataatat tgattcccgc
     5161 tggcgaccac aaagcgaatg ccttgtgctt tcatttgctg atactgagcc ataaaccgct
     5221 cacggttata ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt
     5281 taatgctcat caactattct ccatcgccgt ccgcgttttg gtatcgggtt tggcgacggc
     5341 tttcgcgaca atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa
     5401 tccataatgt tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc
     5461 ggtcgttgct accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga
     5521 aatggtcagc gggaagccca gcgaggcacc cagtccccag agtacaacag acaccccagc
     5581 gacccaggcg ctatcgacaa aaataatcag cccaataccc aacgccccca ttagtgcact
     5641 ggcccgaacc acggcaacgc gactgtaacg gtcgatgaac caaccgccag tgaagcgtcc
     5701 aacggtcatc cctagggtaa aaccggcata aatcagcgag ccggaagtag ggctaaaacc
     5761 gtgaccgtca accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat
     5821 cgccagcact acaacaccaa tcaacagcaa ctggatatcg cgataaaaag gaatgccttt
     5881 ttcgccatgc tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat
     5941 ggcgatataa ataggcgcga tacctaccag cgccgccaat aaaatgtgca ccgttgccgg
     6001 aacgccaaag gccgtcagtg ccatcccgac accagcgcct gccagcgtgc ctaggctata
     6061 aaaaccgtgc atcatcggca aaaccgtttt attcatttct cgctcaacgg cggcaccttc
     6121 aacgtttatc gccacttccg cagaaccaaa acttgcccca aagacgccga gaccaacggc
     6181 aaagagcagg ggcgatgtca gccagagtgc cagacttaat atcatcatcc cgatcaatgc
     6241 gcaggacatc gtgaccagga tgacattacg tgtcccaaag cgtttcacta accacgccga
     6301 gcagagaata ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc
     6361 gatcgagaca gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa
     6421 caggcctggc aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg
     6481 tgaagaattt acggtcatag atcacgtcaa aataagaaga gggaagacaa cattagcaag
     6541 gttgtgtaca tttgtacaca attgcagaca gaggaaatga catgcgtcgc gctaacgatc
     6601 cgcaacggcg agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac
     6661 atgctgttac gcaccgcaaa attgctaccc ttgccggggt accgttggga tcgatgacct
     6721 actatttttc aggaattgat gagttgttgc tggaggcgtt cagcagtttt actgagatta
     6781 tgtcccggca atatcaggca ttttttagcg atgttagtga tgctcaaggc gcatgccagg
     6841 ctatcaccga tatgatctac agctcacagg ttgcaacgcc ggataatatg gagctgatgt
     6901 accagctcta cgcgctggct agccgaaaac cgctattaaa aacggtaatg caaaactgga
     6961 tgcagcgcag tcagcaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg
     7021 atgcgtttat tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcgcgcg
     7081 aggagatttt gaggatggtt gagagggttg cagggtagta gataaatttt agataacaaa
     7141 aaacccatca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc
     7201 aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc
     7261 aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg
     7321 atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cgtaggtgcc cgcatagccc
     7381 agcgcaggga tgttactgcg agctgtatca ctgatgatct ccattgccgg cgcgcaggtg
     7441 cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg
     7501 aacaagaaac agataaccac gggcacaaga ctgacaatta atccggcaat caacatctga
     7561 ccgccaatcg cgcccaggcc gttattaata ccgctaccgg cgctcagacc aacgcctgcc
     7621 ataaacacca tcaagccgaa ctctttcacc atgcttaatg caccttgcgg aatgtaaccg
     7681 aaggtcgggt ggttagcacg cataaagccc agcataattc cggcgaataa caacccggca
     7741 gcgttcccca tgccgaaact gaatgtgctg aactggaagg tgatcatccc gatcatcagc
     7801 ccaataacaa agaaggcgca aaatgccagc aggtcagtga cctggctgtg aatcgagata
     7861 aagccgatgc gatcggcgat ggtttttacg cgacgggcat caccgctgac ttgtaaaacg
     7921 tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct acgaatgacg
     7981 cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt acctacagcg
     8041 ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg
     8101 aaaacttctt taccgttacg gaagctggga tcgagtcggg catgggcgtc gggatagcct
     8161 accaacgcta tttcatcgcc catttgtagc acggcatcac cgtctggatt tgccagaatc
     8221 ccgttacgtc gaatacgttc aatgtagcag ccggtttgtc gataaatacc cagttcacgc
     8281 agatttttgc cgtcggtcca ggccaccagc tccgggccga cgcgataggc gcggatcacc
     8341 ggtaaataaa ccttacggtt ggcatcagtg tccaggccac gttcgcgggc gatttgctgg
     8401 gcgctggtct gtaagtcctg atgctgcaat ttcggcaagt aacgcgcacc aacaatcaaa
     8461 ctcaccagac cgattaaata ggttaaggca tacccgaggc tcagattatc cagtgccagt
     8521 gagagctgcc tgctttccat gccggaatga cgcagtgtat cgccagcacc gaccagaacc
     8581 ggtgtcgacg tcatagagcc tgctaacata ccggccgtca ggccaatatc ccagccaaac
     8641 agcttaccta accctaaggc gatcaccagc gcactgccaa ccatcaccag tgctaacatt
     8701 aggtaatttt tcccatcgcg aaaaaaaatg gaaaaaaagt tcggtccggc ttcgaccccg
     8761 acgcagaaaa taaacagcat aaagccaaga ttaagcgcat cggtgttaat gctgaaatgt
     8821 tgttggccta ataacagcga tacgactaaa acgccaatgg aattacccag ttggatcgaa
     8881 ccaagtcgta actttccgag acatagccca agcgtgagga ccacaaataa taacagaatg
     8941 taattcccat ttaacaattc ggcgacgttt atattcacgg aggctaactt cttgtttact
     9001 agtaagctgt tgaaagaaat ggtaatttac gataatgttt tttaccagaa ttcagggcgc
     9061 agattcattc agcgcaccta aacgatagta aagtaacaat atattttact agtgtaatca
     9121 cattaggtat caacggctat atgaattgcg ttggcctata ttagcatgga atgcgaagcg
     9181 gctttatctt actgaacgcc acactggcga aaaatgtgtt cgatagacgc agtgtcagga
     9241 ggaacgagtg aaacataaac aacgttgggc gggggcaatc tgctgttttg tcctcttcat
     9301 tgtggtgtgc ctttttctgg cgacgcacat gaaaggcgct tttcgggctg ccgggcatcc
     9361 tgaaatcggc ttgctgtttt tcattcttcc tggagcagtt gccagtttct tttcacagcg
     9421 tagagaagtc ctgaaacctc tatttggcgc aatgctggcg gcaccctgtt caatgctcat
     9481 tatgcggctg tttttttcac cgacgcgctc attctggcaa gagctggcat ggttactaag
     9541 cgcggtgttc tggtgtgcgc tgggggcact gtgtttctta tttatcagta gtttgtttaa
     9601 accacagcac agaaaaaatc agtaaagccc tcaacgcgag ggcttgtcag acgatcaggc
     9661 gtccagattt tctttcaccc atgcagcaaa atcggtatag ccgccgatat gttgctgatc
     9721 gacaaaaatc tgcggcacgg tttctacggg tttacctgcc ttttgttgta gatcttcttt
     9781 agtgatccct tccgcacgaa tatctacata ctgatactga aaatcatcgc gttcattgct
     9841 caatttctca gccagatctt ttgcacgcac acagtaaggg caacccgaac gaccaaaaat
     9901 aacggtttgc attatttctc tcctcataga tttatgcctg taatgatcac gctaaaatgt
     9961 attcgctgaa agtaggttta acctgttgca ttaattgcta aaagctataa ctgttaaaca
    10021 caatacagtg aaaagtttta gactgaaggc tcactttgca gagggaagcg tatgcgcgcg
    10081 atcggtaaat tgcctaaagg cgtgttgata ctggaattta tcggaatgat gctactggcg
    10141 gtggcgctgc tgtcggtaag cgactccctg tcgctgcctg agccattttc tcggccagaa
    10201 gtgcagattc tgatgatttt tctcggtgtt ttgctcatgc ttcccgctgc ggtggtggtt
    10261 attcttcagg tggcaaaacg tcttgcccca cagctgatga accgtccacc gcaatattca
    10321 cgttcagaaa gagaaaaaga taatgacgcc aaccattgaa cttatttgtg gccatcgctc
    10381 cattcgccat ttcactgatg aacccatttc cgaagcgcag tgtgaggcga ttattaacag
    10441 cgcccgtgcg acgtccagtt ccagtttttt gcagtgtagt agcattattc gcattaccga
    10501 caaagcatta cgtgaagaac tggtgacgct gaccggcggg caaaaacacg tagcgcaagc
    10561 ggcggagttc tgggtgttct gtgccgactt taaccgccat ttacagatct gtccggatgc
    10621 tcagctcggc ctggcggaac aactgttgct cggtgtcgtt gatacggcaa tgatggcgca
    10681 gaatgcatta accgcagcgg aatcgctggg attgggcg
//
LOCUS       DAZOXR010000149        10687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZOXR010000149 DAZOXR010000000
VERSION     DAZOXR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.152"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUNN_003280"
     CDS             complement(<1..784)
                     /locus_tag="ACHUNN_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0577577.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUNN_003281"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUNN_003281"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0577578.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUNN_003282"
     CDS             1860..2747
                     /locus_tag="ACHUNN_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0577579.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUNN_003283"
     CDS             2780..3790
                     /locus_tag="ACHUNN_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR0577580.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUNN_003284"
     CDS             3860..5293
                     /locus_tag="ACHUNN_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0577581.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUNN_003285"
     CDS             5392..6741
                     /locus_tag="ACHUNN_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0577582.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUNN_003286"
     CDS             6731..7618
                     /locus_tag="ACHUNN_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0577583.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUNN_003287"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUNN_003287"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0577584.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUNN_003288"
     CDS             8352..9224
                     /locus_tag="ACHUNN_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0577585.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUNN_003289"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUNN_003289"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0577586.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUNN_003290"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUNN_003290"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0577587.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2533 c         2855 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccggg taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAZOXR010000150        10591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZOXR010000150 DAZOXR010000000
VERSION     DAZOXR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.153"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUNN_003291"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUNN_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0577588.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="ACHUNN_003292"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="ACHUNN_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0577589.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="ACHUNN_003293"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="ACHUNN_003293"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0577590.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="ACHUNN_003294"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="ACHUNN_003294"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0577591.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="ACHUNN_003295"
     CDS             complement(5254..6294)
                     /locus_tag="ACHUNN_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0577592.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="ACHUNN_003296"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="ACHUNN_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0577593.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUNN_003297"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUNN_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0577594.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="ACHUNN_003298"
     CDS             7229..7669
                     /locus_tag="ACHUNN_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0577595.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUNN_003299"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUNN_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0577596.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUNN_003300"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUNN_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0577597.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10591
                     /gene="rsxC"
                     /locus_tag="ACHUNN_003301"
     CDS             8898..>10591
                     /gene="rsxC"
                     /locus_tag="ACHUNN_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0577598.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2429 a         2736 c         2703 g         2723 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc g
//
LOCUS       DAZOXR010000151        10430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZOXR010000151 DAZOXR010000000
VERSION     DAZOXR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.154"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUNN_003302"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUNN_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0577599.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUNN_003303"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUNN_003303"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0577600.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUNN_003304"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUNN_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR0577601.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUNN_003305"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUNN_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0577602.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUNN_003306"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUNN_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUNN_003307"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUNN_003307"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0577603.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUNN_003308"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUNN_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0577604.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUNN_003309"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUNN_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0577605.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUNN_003310"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUNN_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0577606.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2550 a         2516 c         2754 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAZOXR010000152        10346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZOXR010000152 DAZOXR010000000
VERSION     DAZOXR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.155"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            25..600
                     /gene="ssuE"
                     /locus_tag="ACHUNN_003311"
     CDS             25..600
                     /gene="ssuE"
                     /locus_tag="ACHUNN_003311"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0577607.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            593..1552
                     /gene="ssuA"
                     /locus_tag="ACHUNN_003312"
     CDS             593..1552
                     /gene="ssuA"
                     /locus_tag="ACHUNN_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0577608.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1549..2696
                     /gene="ssuD"
                     /locus_tag="ACHUNN_003313"
                     /pseudo
     CDS             1549..2696
                     /gene="ssuD"
                     /locus_tag="ACHUNN_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2708..3499
                     /gene="ssuC"
                     /locus_tag="ACHUNN_003314"
     CDS             2708..3499
                     /gene="ssuC"
                     /locus_tag="ACHUNN_003314"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0577609.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3496..4263
                     /gene="ssuB"
                     /locus_tag="ACHUNN_003315"
     CDS             3496..4263
                     /gene="ssuB"
                     /locus_tag="ACHUNN_003315"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0577610.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4470..7082)
                     /gene="pepN"
                     /locus_tag="ACHUNN_003316"
     CDS             complement(4470..7082)
                     /gene="pepN"
                     /locus_tag="ACHUNN_003316"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0577611.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7348..8550
                     /gene="pncB"
                     /locus_tag="ACHUNN_003317"
     CDS             7348..8550
                     /gene="pncB"
                     /locus_tag="ACHUNN_003317"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0577612.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8723..10123
                     /gene="asnS"
                     /locus_tag="ACHUNN_003318"
     CDS             8723..10123
                     /gene="asnS"
                     /locus_tag="ACHUNN_003318"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0577613.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2314 a         2677 c         2805 g         2550 t
ORIGIN      
        1 aacaattggt tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt
       61 ccttctcgct ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctggatgta
      121 gaggtttatc actggaatct gcaaaacttc gccccggaag atctgcttta cgctcgtttc
      181 gatagtccgg cactcaagac cttcaccgaa cagctgcaac aggctgatgg actgatagtc
      241 gctacacctg tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg
      301 ccagagcgcg ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg tacggtggct
      361 catctgctgg cggtcgatta tgcccttaag ccagttttaa gtgcgatgaa agcccaggag
      421 ctcctgcacg gcgtgtttgc cgatgactca caagttattg attaccatca caaaccccag
      481 ttcacgccaa atctgcaaac ccgtcttgat accgcgctag aaactttctg gcaggcattg
      541 caccgccgcg atgttcaggt tcctgacctt ctgtctctgc gaggtaatgt ccatgcgtaa
      601 catcattaaa ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga
      661 atcctcgcct gaagcgttac gtataggcta tcagaaaggc agtattggta tggtattggc
      721 aaaaagccac cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt
      781 ccccgcgggt ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac
      841 cggggatatt ccgccaatct ttgcccaggc tgccggggct gatttgatat atgtgggcgt
      901 cgagccaccg aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac
      961 cgtagccgat cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct
     1021 tttactgcgt gcactgcgtc aggccggact taagtttact gacatccagc ccacttatct
     1081 gacgccagct gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg
     1141 ggatccctac tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac
     1201 cgatctcaat caaactggat cgttttatct ggcagctcgc ccctatgcag aaaaaaacgg
     1261 cgcttttatt cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca
     1321 gcgcgagcaa agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc
     1381 ctcgtatctg gatcatcgtc ctcccaccac catcaaaccg gtgagcgctg aagttgccgc
     1441 cttacagcag caaacggcag atctgtttta tgaaaaccgt ctggtgtcga aaaaagttga
     1501 tattcgccag cgcatctggc agcccactca cctggaagga aaacaattat gagtctgaat
     1561 atgttctggt ttttaccgac ccatggtgac gggcattatc ttggaacgga agaaggttca
     1621 cgcccggttg atcacggcta tctgcaacaa attgcgcaag cggcggatcg tctgggctat
     1681 accggtgtgc tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt ggcggcgtcg
     1741 atgatcccgg tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca
     1801 cctaccgttg ccgctcgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg
     1861 tttaatctgg tcacaggcag cgatccacaa gagttggcag gcgacggcgt gttccttgat
     1921 catagcgagc gctacgaagc ctcggcggaa tttacccagg tctggcgggg cgcttattgc
     1981 tgggggaaag cgtcgatttc aacggtaaac atattcatgt gcggggagca aaactgctct
     2041 tcccgccgat tcaacagccg tatcctccgc tttactttgg cggatcgtca gatgtcgccc
     2101 aggagctggc ggcagaacaa gttgatctct acctcacctg gggcgaaccg ccagagctgg
     2161 taaaagagaa gatcgaacaa gtgcgggcga aagctgccgc gcatggacgc aaaattcgtt
     2221 tcggtattcg tctgcatgtg attgttcgtg aaactaacga cgaagcgtgg caggccgccg
     2281 agcggttaat ctcgcatctt gatgatgaaa ctatcgccaa agcacaggcc gcattcgccc
     2341 ggacggattc cgtaggacaa cagcgaatgg cggcgctaca taacggcaag cgcgacaatc
     2401 tggagatcag ccccaattta tgggcgggcg ttggcttagt gcgcggcggt gccggaacgg
     2461 cgctggtggg cgatggtcct acggtcgccg cgcgaatcaa cgaatacgcc gcgcttggca
     2521 tcgacagctt tgtgctttcg ggctacccgc atctggaaga agcgtatcgg gttggcgagt
     2581 tgctgttccc acatctggat gtcgccattc cggaaattcc ccagccgcag ccgctgaatc
     2641 cgcaaggcga agcggtggcg aatgatttta tcccccgtaa agtcgcgcaa agctaaggaa
     2701 cacgatgatg gcaacgccag tgaagaagtg gttattgcgc gttgccccct ggtttttacc
     2761 ggtgggcatc gtggcggtgt ggcaactggc ctcctcggtt ggctggctgt cgacgcgtat
     2821 tttgccttca ccggaaggag tggtgacggc gttctggacg ctctccgcca gcggcgaatt
     2881 gtggcagcat ctggcgatca gttcctggcg ggcgctgatt ggcttttcaa ttggcggatc
     2941 gctgggcttg attctgggac tgattagcgg gctgtcacgc tggggagaac ggctgctgga
     3001 tacctcaatt cagatgttgc gcaacgtacc gcatctggcg ctgatcccgc tggtgatttt
     3061 gtggtttggc attgatgaat ccgcaaaaat ctttctggtg gcgctcggca cgctattccc
     3121 catttatatc aacacctggc atggcatccg taatatcgat cgcggtctgg tggaaatggc
     3181 gcgtagctat ggattatccg gcataccgct gtttatccat gtgatcctgc ctggtgccct
     3241 gccctcaatt atggtcggcg tgcgttttgc gttgggcctg atgtggctga cgctgattgt
     3301 tgccgaaacc atttctgcca attcaggcat tggttatctg gcgatgaatg cgcgggagtt
     3361 tttgcaaacg gacgtggtgg tagtcgctat tattctttac gccctgctcg gcaaacttgc
     3421 tgacgtcagc gcgcagctgt tagagcgcct ctggttgcgc tggaacccgg cttatcattt
     3481 gaaggaggcc acggtatgaa tactgctcgt ctgaaccagg gcacaccatt gttgctcaat
     3541 gcagtaagca aacattacgc ggaaaatatc gtcctgaacc aactggattt acatattccg
     3601 gcaggtcagt ttgtggcggt ggtgggccgc agcggtggtg gcaaaagtac cctgctgcgc
     3661 ctgctggcag gtctggaaac gccaaccgca ggcgatgtgt tagcgggcac cacaccgctg
     3721 gctgagattc aggaagacac gcgaatgatg tttcaggatg cgcgtctgct gccgtggaaa
     3781 tcggtgattg ataacgttgg gttaggcctt aaaggccagt ggcgcgatgc cgcgcgtcgg
     3841 gcgttggctg cagtaggact ggagaatcgc gccggggaat ggcctgccgc actttctggc
     3901 gggcagaaac agcgtgtggc gctggcaaga gcgttgattc atcgaccggg tttattgttg
     3961 cttgatgaac cgctcggggc actggacgcc ttaacgcgac tcgagatgca ggatttgatt
     4021 gtgtcacttt ggcagcagca cggctttacc gtactgttgg tgacgcatga tgtgagcgaa
     4081 gcggtagcaa tggctgaccg ggtgttgtta attgaagagg gaaaaattgg tctggatttg
     4141 acagtggata tcccccgtcc gcgtcgatta gggtcggtgc gattagcgga gctggaagcg
     4201 gaagtattgc aacgggtaat gcggcgtggg cattctgaac aactgattcg gcgtcatggt
     4261 taatatgccg gatgtggcgc acaaaaccag cattttcaac ggacttgtag gcctgataag
     4321 acacgtcaag catcgcatca ggcagttggg cacggctgcc ggatgcggcg tgaacgcctt
     4381 atccagccta caaaaccagc aatttcaacg gagttgtagg cctgataaga cgcgtcaagc
     4441 gtcgcatcag gcattttggc tcaaaagtat caagccagtg ctttggtgat cttctcgtac
     4501 agatcgccag agagattttc cagccctttc aactgttcca gcgccgcgcg cattttctcc
     4561 tgacgtttgg catcataacg tttcaggcga atcagcggtt caatcagacg tgaagctacc
     4621 tgcgggttac ggctgttgag gtcggtaagc atttcctcca ggaactggta accgctgcca
     4681 tcttcggcat ggaacgctgc cgggttgctg cccgcaaacg caccaatcaa cgaacgaata
     4741 cggttcgggt tgctcatggt aaatgagcga tgctgcaaca gaccacgcac cgtctccagc
     4801 acattcgccg ccgggctggt ggcttgcagg ataaaccatt tatccatcac cagaccgtcc
     4861 tgatgccact tatcgtcgta ctcctgcatc agcgcgtcac ggcaaggcag ctgtgcagca
     4921 accgccgcag aaagcgctgc cagcgcatcg gtcatattgt ttgcttcatg gtattgcttg
     4981 ctcaccagca cgtcagccag atgcgtttca ccaaaagcaa ggaagcgcag gcaggcattt
     5041 cgcagagtgc gttttgcaat atcttcatgc tcaacacggt actcgctctg gtaattcgcg
     5101 ttgtaaatag ccagcagctc atccgccagt tcagtcgcca gagtacgagt gagtgcttcg
     5161 cgtacttcgg caatagcaat cgggtcgatg atatcgaaca gttcagccat ttcattgaca
     5221 gaaggcagcg tcaggatttc tgccgccagc gccggatcaa tcttctcatc gagcagcacc
     5281 gcgcggaaag cgtcagccac atgtaccggc agagacagcg gctgcccttg ctgatgacgg
     5341 gcgacgttca gcttgatgta ggttgccagc aaactttgcg ccgcatccca acgggagaaa
     5401 tcattacgcg catgacgcat caggaaggtc agttgctgat cactccactt atattccagt
     5461 ttcactggcg cagagaattc gcacagcagc gcaggcaccg gctggaagta gacattatca
     5521 aaaacaaagg tctgttccgc ctgggtgacg ttcagcacgg aattcaccgg atgaccgcct
     5581 ttctgcaacg ggatcacttt gccttcgttg tcatacaatt cgatggcaaa cggaatatgc
     5641 agcggctgtt tttctgcctg atccggcgtg gctggcgtgc gctggctgat ggtcagggtg
     5701 tactgctcag tttccggatt gtagtcgtct ttgacggtca caatcggcgt accggactgg
     5761 ctgtaccaac ggcggaaatg ggagagatca acattcgacg catcttccat cgcctgcaca
     5821 aagtcgtcac aggtcgctgc gctaccatca tgacgctcga aatagagttg catccctttc
     5881 tggaagtttt cttcgcccag cagggtgtgg atcatacgga tcacttccgc gcccttctcg
     5941 taaacggtca gggtgtagaa gttgttcatc tcaatgacca tgtccgggcg gattgggtgc
     6001 gccatcgggc tggcgtcttc cgcaaactgc aatccgcgca tggtgcgcac attattgatg
     6061 cggttaactg cgcgggaacc aaggtcagag ctgaattcct gatcgcggaa gacggttaaa
     6121 ccttctttca ggctgagctg gaaccagtcg cggcaggtca ctcggttacc ggtccagtta
     6181 tggaaatatt catggccgat aacgcgttca atatcgaggt aatctttgtc ggtggcggtg
     6241 tcggtgcggg ccagcacata tttggagtta aagatattca gacccttatt ctccattgcg
     6301 cccatattga agaaatccac cgcgacgatc atatagatgt cgaggtcata ctccaggccg
     6361 aagcgttctt catcccattt cattgagttt ttcagcgagg tcatcgccca cggcgcgcga
     6421 tcaaggttgc cgcgatcgac gtacagctcc agcgccactt cgcgaccaga acgcgtggta
     6481 aaggtgtcgc gcagtacatc aaagtcgcct gccaccagcg caaacaggta gcacggtttc
     6541 gggaacgggt cctgccactg tacccaatgg cgtccgtttt ccagttcacc ttgcgcaacg
     6601 cggttaccgt tggaaagcag gaagggatat ttggttttat cggcaataat tttggtggta
     6661 aaacgcgcca gcacgtccgg gcggtcgaga taatacgtaa tatggcggaa accttcggct
     6721 tcacactggg tgcaaagcgc atcgcctgac tgataaagcc cttccagcgc ggtattcgcc
     6781 gccgggctta tttcattaac gatcttaagc gtaaaatgct ccggcaaatt actgatgacc
     6841 agtgcgccct cttcttcttt ccaggcggtc cacggctcat cattaatatg aacagaaacc
     6901 agtttgaggt cttcgccgtt gagacgaagt ggagcatctg atgcaccatg acggacagcc
     6961 tggctgaccg cggtaacgac cgtcttttgc gcgtcgaggt caaaggtcaa gtcaatatca
     7021 gtaatctggt aatctggcgc acgataatcg tgacggtatt tggcttgtgg ctgttgagtc
     7081 ataaataacc tttagcatct tttatagagt ctggtgttca gtctattcct gttgcgtaaa
     7141 tcgcgctatg cagaatcttc atcttttcag gtacaaacgc ctttattgct acatttttat
     7201 aacatacacc gcgtaatgcc atcgaccaga aaggtggcat atggtgtgat cggggttcaa
     7261 taaattgcga aacaaggtat actccagcag ttcctgaaga tgtttattgt actaaacgct
     7321 cctgtacgag gacgctactg cgcacctatg acacaattcg cttctcctgt tctgcactcg
     7381 ttgctggata cagatgctta taagttgcat atgcagcaag ccgtgtttca tcactattac
     7441 gatgtgcatg tcgcggcgga gtttcgttgc cgaggtgacg atctgctggg tatttatgcc
     7501 gatgctattc gtgaacagat tcaggcgatg cagcacctgc gcctgcagga tgatgaatat
     7561 cagtggctct ctgccctgcc tttctttaag gccgactatc ttaactggtt acgcgagttc
     7621 cgctttaacc cggaacaagt caccgtgtcc aacgataatg gcaagctgga tattcgctta
     7681 agcggtccgt ggcgtgaagt catcctctgg gaagttcctt tgctggcggt tatcagtgaa
     7741 atggtacatc gctatcgctc accgcaggcc gacgttgcgc aagccctcga cacgctggaa
     7801 agcaaattag tcgacttctc ggcgttaacc gccggtcttg atatgtcgcg cttccatctg
     7861 atggattttg gcacccgtcg ccgtttttct cgcgaagtac aagaaaccat cgttaagcgt
     7921 ctgcaacagg aatcctggtt cgtgggcacc agcaactacg atctggcgcg tcggctttcc
     7981 ctcacgccga tgggaacaca ggcacacgaa tggttccagg cacatcagca aatcagcccg
     8041 gatctagcca acagccagcg agctgcactt gctgcctggc tggaagagta tcccgaccaa
     8101 cttggcattg cattaaccga ctgcatcact atggatgctt tcctgcgtga tttcggtgtc
     8161 gagttcgcta gtcggtatca gggcctgcgt catgactctg gcgacccggt tgaatggggt
     8221 gaaaaagcca ttgcacatta tgaaaagctg ggaattgatc cacagagtaa aacgctggtt
     8281 ttctctgaca atctggattt acgcaaagcg gttgagctat accgccactt ctcttcccgc
     8341 gtgcaattaa gttttggtat tgggactcgc ctgacctgcg atatccccca ggtaaaaccc
     8401 ctgaatattg tgattaagtt ggtagagtgt aacggtaaac cggtggcgaa actttctgac
     8461 agccctggca aaactatctg ccatgataaa gcgtttgttc gggcgctgcg caaagcgttc
     8521 gaccttccgc atattaaaaa agccagttaa tatcatcagg gagctaatcg gctccctttt
     8581 tttaccttta attccgaaat ctttcgctgc atttgcgaat tctgcttgtc tgattgcaga
     8641 tgccaggtaa cataggtatc ccccccccca tttacgggtg gacaagtgtt tattttttcc
     8701 gactattaac agagagaata ttatgagcgt tgtgcctgta gccgacgtac tccagggccg
     8761 cgtagccgtt gacagcgaag tcaccgtacg cggatgggta cgtacccgcc gagattcaaa
     8821 agctggcatc tccttcctcg ccgtttatga cggttcctgc tttgatcctg tacaggctgt
     8881 catcaataat tctctgccca attacaatga agacgtcctg cgtctgacca ccggctgctc
     8941 ggtcattgtg acgggtaaag tcgtggcatc gccgggccag gggcaacaat ttgaaattca
     9001 ggccagcaag gttgaagttg ctggttgggt tgaagatcca gacacttacc cgatggcggc
     9061 aaaacgccac agcattgagt atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct
     9121 gattggtgcc gtcgcgcgcg ttcgccatac gctggcgcag gcgctgcatc gcttctttaa
     9181 cgagcaggga ttcttctggg tttcaacgcc actgattacc gcatctgata ccgaaggtgc
     9241 aggcgaaatg ttccgcgttt ctacgctgga tctggaaaac ctgccgcgta acgatcaggg
     9301 caaagtggat ttcgacaaag acttctttgg taaagagtct ttcctgaccg tatctggcca
     9361 gttgaacggc gaaacctacg cttgcgcatt atccaaaatt tataccttcg gcccgacttt
     9421 ccgtgctgaa aactccaaca ccagccgtca cctggcggaa ttctggatgc tggagccgga
     9481 agtggcgttt gctaacctga acgatatcgc gggtctggct gaagccatgc tgaaatatgt
     9541 cttcaaagcg gttctcgaag aacgcgcaga cgacatgaaa ttcttcgctg aacgcgtaga
     9601 taaagatgcc gtttcacgtc tggaacgctt cattgaggcc gatttcgcgc aggtggatta
     9661 caccgacgca gtgaccattc tcgaaaactg cggcaggaag tttgaaaacc cggtttactg
     9721 gggcgtcgat ctctcttctg agcatgagcg ttatctggcg gaagaacact ttaaagcacc
     9781 ggtagtggtt aaaaactatc cgaaagatat taaagcgttc tatatgcgcc tcaacgaaga
     9841 cggtaaaacc gttgcggcta tggacgttct ggctccgggc atcggtgaga tcattggtgg
     9901 ctcccagcgt gaagaacgtc tggacgttct ggacgagcgt atgctggaaa tgggcctgaa
     9961 taaagaagat tactggtggt atcgcgatct gcgtcgctac ggtactgttc cgcattcagg
    10021 tttcggtctt ggttttgaac gcctgattgc ttacgtaact ggcgtgcaaa acgtgcgtga
    10081 tgtgattccg ttcccacgta ctccgcgcaa cgccagcttc tgattttctt cctcaaagag
    10141 ccagcgcctg ctggctcttt tttatccttt ctgtcaagtt atctgtttgt taagtcaagc
    10201 aatctatttg caaccccgcc ataacgatca tcctgttacg gaatattaca ttgcaacatt
    10261 tacgcacaaa aaataacccg cattcttatt gcggattagt tttttcttag ctgatagcac
    10321 aattttcata ctattttttg gcattc
//
LOCUS       DAZOXR010000153        10287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZOXR010000153 DAZOXR010000000
VERSION     DAZOXR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.156"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            202..549
                     /locus_tag="ACHUNN_003319"
     CDS             202..549
                     /locus_tag="ACHUNN_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR0577614.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(569..1159)
                     /locus_tag="ACHUNN_003320"
     CDS             complement(569..1159)
                     /locus_tag="ACHUNN_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577615.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLQAEQKRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(1159..1416)
                     /locus_tag="ACHUNN_003321"
     CDS             complement(1159..1416)
                     /locus_tag="ACHUNN_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577616.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            1985..2398
                     /locus_tag="ACHUNN_003322"
     CDS             1985..2398
                     /locus_tag="ACHUNN_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577617.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2400..3179
                     /locus_tag="ACHUNN_003323"
     CDS             2400..3179
                     /locus_tag="ACHUNN_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0577618.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLSPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            3359..4003
                     /locus_tag="ACHUNN_003324"
     CDS             3359..4003
                     /locus_tag="ACHUNN_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFR0577619.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            4090..4398
                     /locus_tag="ACHUNN_003325"
     CDS             4090..4398
                     /locus_tag="ACHUNN_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFR0577620.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            4812..5792
                     /locus_tag="ACHUNN_003326"
     CDS             4812..5792
                     /locus_tag="ACHUNN_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR0577621.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            5785..6201
                     /locus_tag="ACHUNN_003327"
     CDS             5785..6201
                     /locus_tag="ACHUNN_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0577622.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(6203..7477)
                     /locus_tag="ACHUNN_003328"
     CDS             complement(6203..7477)
                     /locus_tag="ACHUNN_003328"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR0577623.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(7477..7914)
                     /gene="umuD"
                     /locus_tag="ACHUNN_003329"
     CDS             complement(7477..7914)
                     /gene="umuD"
                     /locus_tag="ACHUNN_003329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0577624.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(7911..8159)
                     /locus_tag="ACHUNN_003330"
     CDS             complement(7911..8159)
                     /locus_tag="ACHUNN_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0577625.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            8553..9479
                     /locus_tag="ACHUNN_003331"
     CDS             8553..9479
                     /locus_tag="ACHUNN_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0577626.1"
                     /translation="MPKWCSNRMYFSGEPAKIAEIKRLASGAVTPFYRRATNEGIQLF
                     LAGSAGLLQTTEDVRFEPCPGLTAAGRGVLSPENITFTRWLKHLQDGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLS
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            9476..9787
                     /locus_tag="ACHUNN_003332"
     CDS             9476..9787
                     /locus_tag="ACHUNN_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577627.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            9863..>10287
                     /locus_tag="ACHUNN_003333"
     CDS             9863..>10287
                     /locus_tag="ACHUNN_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFR0577628.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNR"
BASE COUNT         2361 a         2540 c         2691 g         2695 t
ORIGIN      
        1 atgacatata cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat
       61 attttctgtt catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat
      121 gacaacataa ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca
      181 tcatggaacg gaggtgctcc tgtgaccttt gagcagaaaa aagcccgggc cattgccctg
      241 atggacagca aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg
      301 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacgctgttg
      361 atggggagct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
      421 tcaggaatgg tggcaggtga ggccattatt ataagtatca cgggtggttt tttgttcgga
      481 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accggctgcc accatgggat
      541 gatgtgtaaa ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc
      601 gaacctctat tcctgcgtca tgacaggcct gcagccactg cgccacttcc agcggatcgc
      661 cctcccggcg taccaccctg ccttctttat tccataactg cagacaggtg ctgccgtcga
      721 gacgcaccac aaaatcccca cggcaggcct gatacgtcat gccgttcagc tctaccgcca
      781 gatcagtggc agaagtgccg tctgcgggca gacctgctgc cgggaccagc ggcagtacga
      841 cgacttctgc cgcacgctgt tctgcccgga ggcgcttctg ttctgcctgc agcagaggct
      901 gtgccacggc ccggccggcg tccgtcagct ccactgccag ctgcagattc ggggctcgca
      961 gggtgcgcag ccagccggca tcctccaggc gacggcagga ggcgcgtagg tttggcccgt
     1021 agaccggggc ttccccgctt cgctccagca cccgttctat gtcccgcgtc agcacggggc
     1081 ctggtcgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc agtggcgacg
     1141 gtcggcggct ctgggtcatt acctgtcatc ctccggccag atgaggtttc tgaggcgctg
     1201 aaagtcggcg tcaatgagct cctgtctctt tatggcattc attgaggcat agccatttat
     1261 ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc agagataaat
     1321 cgcgccctgg ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc gggtcagttg
     1381 ttcatagctg agtgaaaacg tcatttcctg atccatcctg cacgctccat ctgataatgt
     1441 tctgacaggt aaaaatcata tcctgcatga tttgtgtgct cctgatttct gataatatca
     1501 gatattgttt aatttttaat gatgaaaatc atatctggta tgattttatg tgaactgctt
     1561 aattatcata tcatttgccc gtttcccgcc gtgcagatcc ggtttccggc ggttacggat
     1621 cttcctctcc ccccacacaa cgccacctcc cgtcagcaca acatgtggtg ccggattcag
     1681 ctgctgatga cactatatgt tgtgtcatct ccctgacctg tgatgcgtcg cgcaggggcg
     1741 gaaaacagcg atatgattat ttcctcagcg tggtacactt ccggaaagtt gtgatattcc
     1801 ggaaagtcgg atctgacgga aacgactctc cggtaattta acggcgtggt tatatggatg
     1861 cttgttatca tggtgatgat gataacggca tgatgttatc tgacggcgtg acggtaaggg
     1921 cagtgatgat ggatgacgtt atcgcatgac cgtccctgcc cggaaaagaa aaaaggagtc
     1981 acccatgttt tttattgaga atgaaggtca ggctgtcgcc ggaacggatt actggcagtc
     2041 tgtacaggcg caggccggat atgtctacct cagctggaat gccggcgcag ccaggctgct
     2101 tgtcccggat gcggcaaaac atttactcag ggagatgcgg ggggctgagt acgtcatcat
     2161 cagtaaggga acgctgcatg gccgcgatgc gctggaactg gtatttgaag acggcagcga
     2221 tgcgccgttt gtgatccaca tgctgagtga gcagtgcgat cgcctgctcc ccgaaaacaa
     2281 ccagggaggg ggttttgttg tcaccgtctg gacgcgtggc ggtaaccagc tccgttatcc
     2341 gggaaaatac cgggttgtgg aaaacctgcc cgacgtttcc ccgtggagtg aacactgata
     2401 tgcagcacct gccggcaccg atccaccatg cccgggatgc tgctcagctt cctgttgcca
     2461 tcgattatcc ggcagcgctg gcactccgcc agatgtcgat ggttcatgat gaactgccaa
     2521 aatacctgct ggcccctgaa gtgagcgccc tgctccatta cgtcccggat ctgcgccgca
     2581 agatgctgct ggccacactg tggaacaccg gtgcgcgcat taatgaagca ctggcgctga
     2641 cgcgggggga tttttcgctt tcgcctccgt atccgtttgt gcagctggcc actctgaagc
     2701 agcggaccga aaaagccgcc aggacggcag gaagaatgcc cgccggtcag cagactcacc
     2761 ggctggttcc gctctccgac gcctggtacg tcagccagct gcagacgatg gtggccacac
     2821 tgaaaatccc catggaacgg cgtaataaac gaacaggcag gacagagaaa gcgcggatct
     2881 gggaagtgac ggacagaacg gtcaggacct ggattgggga ggcggttgcc gccgctgccg
     2941 ctgatggtgt gacgttctct gtcccggtca cgccacatac gttccgccat tcctatgcga
     3001 tgcacatgct gtatgccggt ataccgctga aggtcctgca gagtctgatg gggcataagt
     3061 ccatcagctc aacggaggtc tacacgaagg tgtttgcact ggatgtggct gcacggcacc
     3121 gggtgcagtt ttcgatgcct gagtccgatg cggtgtctat gctcaaaaga attccataag
     3181 ctctgacttt tttaaatccc tttcggatat ctcatggata tattatcggt atttcatatt
     3241 ctgttgacat gcaggaccgg tatagagtaa aaaaacatct attgagtacc tcttggatat
     3301 ccgaaaggtg tcttatgggg tctttatggg tattacatgt ggattctgga gatacactat
     3361 gcctgttatt gctaatacgc atcccaaagg tggtgtgggt aagacaactt catccgtaaa
     3421 cattgttggt gaaatgaagt ctgatacagt tgatctggat actcataccg ggctttctat
     3481 cattctgggg ctgagacctg aaggaaaaga aatttccgtg aaagtaccca aaacagtgga
     3541 tgagttaatc gaaattatga ctccctacaa gaacagcgat aagacgttac ttattgactg
     3601 tgggggattt gactcagatc ttacccgtac tgcgattgcc tttgcagact gcgttattgt
     3661 cccttcaaaa gactccctga ctgaacgtat tggtttgatg cattttgatg gagtactgga
     3721 cgaaatcagc tctatcatgg ggaccgatat aactgctcac ctgtatctct gtaaagttaa
     3781 tcctaacaag aagaaattcc ctaagcttga tgcaatcctg ccatcgttca aacatctgaa
     3841 gctgatgaag agccgcattt ctgctcgtgc agaatttgag gatgtcattg agactgggat
     3901 gggaatcact gagtctgttc atgggcgcta ttcggcagga ggtaaggaag ttattgccct
     3961 gatcgaagaa atcaatcatt tgattgaaaa taacaaacag taaggtgtgt ctattgggta
     4021 tccatatagt atcctttggg cttttaatgg atatctttta agtatctaac aaacatcctg
     4081 ggttatctca tgagcaaagc gaaatttagt gcacaggctg aaaaactgaa aaacctggtg
     4141 gattctgcca gattacaaac ttcttcttct gccgtaacag caacagcccc gtctcagcca
     4201 aatgaaggtg cagaggaggt ttcaggtcgt cccgtggctg ccaggacgaa ggctaagaat
     4261 cttaaggcca taccgctgtc gtattttgaa gctcatgcgg aacttaaagc gaccagtaaa
     4321 acatctctgg attttagctc atatattatt gaggctataa gagagaagct tgaacgtgat
     4381 ggtgcaatag gtcaataaac tatcggatac cttaagggta tctgatgtgt gtcttttaaa
     4441 tgcgttatgg ggattcttca cgtacctaaa gagtccccat tagacatctt tctgtttatg
     4501 gtgacatgat tcgtcactgc tttcctttcc tctcagtttc accatttcat tccttttccc
     4561 ttaacctgaa ccatgatgtc cacgatgttt ttatattgca tttatgtatc acatataacc
     4621 ttcataatgt ttttgtatct atgatttcct ctggtcttaa agttgtgtgt acaatgtaca
     4681 tgcaatttat cgtgttttta tgttgcattt gtcctttcag aaagaccgtt ttttcattat
     4741 aattgaatat acattgcatg tatgaagcat gtttgttttt tctatttgtt tatgagcaac
     4801 ggagactggt tatgaaaatc tttattgatg atggttctac caacattaaa cttgcctggt
     4861 tggaggatgg tgacgtgaaa acgttaatca gccctaacag ctttaaaccg gaatggtctt
     4921 ttagtttact ggatgatgct gctccggcta attacgagat cgacggcgag aagttttcat
     4981 ttgatccatt aagtgctgat gctgtcgtaa cgactgaaac acgctatcag tacagtgatg
     5041 tgaatgttgt ggccattcag catgcgctac agcagaccgg cctgaaagcg cagcctgttg
     5101 atgtcatcgt cacattgccg atctctgaat accttgacgc caacaatcag aaaaataagc
     5161 aaaacattga aagaaagaaa aaaaatgtga tgcgtgaagt acgggtgcag gggagtgatg
     5221 catttgttat tcgcagtgtt tccgttcttc ctgaaagtat tcccgctggt ttcagtgttc
     5281 ttgcggggct tgaggatgat gaatcccttt tgatcgtaga tctcggtggt acaactcttg
     5341 atgtgtcgca tgttcgtagc aaaatgacag ggattacaaa aacctggtgt gatccgaata
     5401 tcggcgtgtc cctgattaca tccggggtta aggaacagat ggctgtacat gctaatacaa
     5461 gagtgagctc attccaggct gacaatatca ttgtgcaccg taatgaacct gactaccttt
     5521 cccgtcgcat ttataatgct gaacagcgtg aatctatcat taatgttatc aatgaacggc
     5581 agaagttgct tattaaacga gttaatgatg tcatcagccg tttcaccgac tatacacatg
     5641 taatgtgtgt tggtggtggg gctgaaattg tggccgaggc tgtgaagaac ctgactaagg
     5701 ttcctgatga gcgcttttat ctgagctcat ccccgcagtt tgatttagtc atgggaatga
     5761 taaaaatgaa aggtggtgtg actaatgagt gatgaaaata aatccagacg ctgctcattt
     5821 gaacttttcc cggatgaacg gacaggagat aagatagcag atgagctcat tgcaaatgaa
     5881 aaactgaagg agcggggacg tttcatgcgc gcgatgttag ttactggtgc ggcgtttgca
     5941 gctatagata aacgtcttcc tttgctgata tcggagttac tgacagaaaa tacgactctg
     6001 gatgatatta ataaggtgat ttccagcgtg ataccaggtg ctttttcggt tgaaaagaaa
     6061 ttgcttgaat tactggaaaa acaatctggt ctccatacgt ctgtggattg cagtactcct
     6121 cttacagagc agagtctgtc gagaaatgac ggtgaagacc agaccagaag aaacgctgaa
     6181 aatatgttcg gagatgactg aactaaccca gcctggccag gggaatatct cgccatttag
     6241 ttgtataaca ctgactcaac atttcccgtt tcatttgcca ttcaggggcg gttcctcgcc
     6301 ccgcaaacca cactttccct tttcccgact ggttcagttc atcgagtgtt ttcattaact
     6361 tttcactgtt tttacggggc tggatttcat caaataatcc cggctgcgct atacccgatg
     6421 gtgtgaaatc agccagcatg actcctgcct tcatatagcg gtacccttca cgccagacat
     6481 ggtttaaggc tctgcatgcg gcggcaataa tgtcccggct gtcctgtgtg ggcaatggaa
     6541 gcttttccac agcggcattg ctgtaacagg gttcttttac tgcaaagggg gatgtccgta
     6601 caaatgtcgt cacctgccgg caatactgac gctccccacg tagtttctct gcggcccgct
     6661 ctgcatactg aacaacagcc tggtgcatgg catctttgtc tgtgattcgt tcaccaaaac
     6721 tgcgactaca gacaatctgc tgttttgccg gtggtgcttc ttccagggat atgcaggact
     6781 cgccgttgag ttcgcgtacc gtacgctcaa gaatgacgct gaagtttttc cggatgaatg
     6841 ccgtgttagc ctgcgccagc tgcagtgctg tgttaatacc cagcgcattc agcttttccg
     6901 tcagtctgcg tcctactccc cagacctcac caactggctg cagccccagt agcttcaagg
     6961 tccgattacg gttttctgcc gtcagcgcga ccacaccgga aaactgtggc cattgctttg
     7021 ttgcccactg tgcactttta gccagcgttt ttgtaggcgc aatgcccacc cccatggtga
     7081 gtcctgtcca gctctttacc tgttccctga gctgatgacc aaaaacctcc ggagagatgc
     7141 aatgatttat cccccgcaaa tcaatgaaca tttcatcaat tgagtagggc tcaactgcgg
     7201 gagaaagcga ctccagaaca gccataaccc gttggctcat gctgtggtac agcgcataat
     7261 tgctggaaaa tacatgtatt ttcttctcca ggcgcatctg tctcacctga aaccagggct
     7321 gccccattct gatgccaagg gcttttgcct ccgggctgcg cgcgatcaca cagccatcgt
     7381 tattgctgag tacgatgacc ggttcgttgc gaaggtccgg gcggaaaact ttttcacatg
     7441 aggcgtagaa actgttgata tcagccagtg caaacatcag cgtaactccc gcgtcctgtg
     7501 tatcacgtga gtgacaacac caaaaataca gatgttttcc ggatacagtg tgcggaactc
     7561 cgggctgtct gaaaccggct ccagtgccgg gcgtgggcgc aacagcagtc gtttgacggt
     7621 gaactcaccg tcgatctcag cgataacgat gtccccgtgt tgtggttttt cggccctgtc
     7681 cactaccagc agatcaccat tctgcacgcc agcctggttc atcgattcac cgctggcgcg
     7741 cagaaagaag gtggctgcag gtctgctgat gcaatagctg ttcagatcca gttcctgctc
     7801 agcataatca gtggcaggcg aaggaaaacc ggcctggcaa cgatcggcaa acaacggtcg
     7861 cacgtaactg tcatcgcctg aagggtcagc cggacggtga taaacggtac tcatgctgca
     7921 ggcagccagc tgtcgtcctg ccagatttct tccagcagag acaggatacg ttctttatcc
     7981 ttgtcgttgc gggctcctgt gacagataca gacggggcgc tgccctggtc tatacgcagc
     8041 cagatttccg ggtagtgtgg tttcagacgt tgttcgattt cattctgtag cgcctgaagt
     8101 gtgcctgatt tgaggttttt tgtgctctgg cggtcgaaaa gaatttcaat gcggatcatg
     8161 ttctcatctc ccttttcttt tgctgtatgt atatacagta ttctaatgac tgaatccaca
     8221 cacagtcaaa tgtcattatt gtgactaaaa aacgctcaat tctctgagag tgtggctgta
     8281 tgcctgctgt ggactgctgt gtccagctta tccacagcat tttgcgcacg gttctgtgaa
     8341 taaaatacct ggttacccag gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt
     8401 gtgtcgctgt cgccggcctc ctcacccggt cacgtttcgt cgttcctcct ccacgcgctg
     8461 cggcttcggg gccgcacctg cattcgtatg cggtcgcccg gttacaggtg cggcacggcc
     8521 tgatggaggc cgtatgtgag aggagaattc ccatgccaaa atggtgctcg aatcgtatgt
     8581 atttttctgg tgaaccggca aagattgctg agattaaacg gctggccagc ggtgcagtca
     8641 caccgtttta tcgccgcgcc acaaatgaag gtattcagct gtttctggcc ggaagtgccg
     8701 gacttctgca gaccactgaa gatgtgcggt ttgaaccatg ccccggactg acggctgccg
     8761 gacgtggtgt tttatcgccg gagaatatca cgttcacccg ctggctgaag cacctgcagg
     8821 acggtgtact gctggatgag caaaactgcc tgatgctgca tgaactctgg ctgcagagcg
     8881 gtactggaca gcgtcgctgg gaagggttac cggatgatgt cagggaaact atcactgtgc
     8941 atttcaccgc aaaaagaggt gactggtgtg acatctgggg taacgaggat gtatcggtgt
     9001 ggtggaaccg tctgtgtgac aacgtactgt cggaaaaaac catgccgttt gacctgctga
     9061 cggttttgcc gacccgcctg gatattgaag tgaatggctt taacggcggt gttctgaacg
     9121 gtgttccttc tgcatatcac tggtatacag aacggtatgg cgtgaagtgg ccttgtgggt
     9181 atgacctgaa tatcagcagt cagggggaaa acttcattca ggtagatttc gacacgccgt
     9241 ggtgtcagcc ggaaagcgac gttgttgcag aattaagccg ccgtttcggc tgcacgctgg
     9301 agcactggta cgccgaacag ggctgcaatt tctgtggctg gcagttgtat gagcgcggag
     9361 agctcgttga tgtgctgtgg ggggaactgg aatggtcttc cccgacagat gacgatgagc
     9421 tgccggaagt caccggacct gcgtggatag tcgacaatgt ggcgcattat ggcggatgaa
     9481 gtatgacgga gacgggcggg caaccgcccg tttcttttcc ggtaaaggat gtcgccgtgc
     9541 tcctcttttt actgcgccgg ctgacgcggc gcggcataaa cgctgcctgt ggtcagtgtc
     9601 ccgcgtccgg cgggcacggg acgggcgctt ttaccgctcc cggctggcgt cggtgacagt
     9661 gtacgccagc ccgtcgccct tttctgatga acgcccttca agccgctttc gcggcatagc
     9721 cttgccgtca aaaagacgac ggctgcggta ttccacggtc ggcctgaccc gttaccggac
     9781 gcggtgaaca gcccacaggc agcggggaac gggcaccaca ggggtgccct cccggtgccc
     9841 tctgtaagag aaggagtttt gtatgtcccg ttttgtcctc ggtaactgca tcgatgttat
     9901 ggcccgtatc cctgataacg ccattgattt catcctcacc gacccgccgt atctcgtcgg
     9961 tttccgtgac cgttccgggc gcaccattgc tggcgataaa accgacgaat ggctgcaacc
    10021 ggcctgtaat gagatgtacc gcgtgctgaa aaaagacgcg ctgatggtga gcttctatgg
    10081 ctggaaccgc gtcgatcgct ttatgtctgc ctggaaaaat gcgggattca gcgttgttgg
    10141 tcacctggtc ttcaccaaaa actacacatc gaaggccgca tatgtgggct atcgccacga
    10201 atgcgcctac atcctggcaa aaggccgtcc acgtctgcca caaaacccgc tgccggacgt
    10261 gctgggctgg aaatattcgg gcaatcg
//
LOCUS       DAZOXR010000154        10161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZOXR010000154 DAZOXR010000000
VERSION     DAZOXR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.158"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..452)
                     /gene="folK"
                     /locus_tag="ACHUNN_003334"
     CDS             complement(<1..452)
                     /gene="folK"
                     /locus_tag="ACHUNN_003334"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0577629.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHT"
     gene            complement(449..1846)
                     /gene="pcnB"
                     /locus_tag="ACHUNN_003335"
     CDS             complement(449..1846)
                     /gene="pcnB"
                     /locus_tag="ACHUNN_003335"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0577630.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1906..2802)
                     /gene="gluQRS"
                     /locus_tag="ACHUNN_003336"
     CDS             complement(1906..2802)
                     /gene="gluQRS"
                     /locus_tag="ACHUNN_003336"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0577631.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2869..3324)
                     /gene="dksA"
                     /locus_tag="ACHUNN_003337"
     CDS             complement(2869..3324)
                     /gene="dksA"
                     /locus_tag="ACHUNN_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0577632.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3502..4206)
                     /gene="sfsA"
                     /locus_tag="ACHUNN_003338"
     CDS             complement(3502..4206)
                     /gene="sfsA"
                     /locus_tag="ACHUNN_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0577633.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4221..4751)
                     /gene="thpR"
                     /locus_tag="ACHUNN_003339"
     CDS             complement(4221..4751)
                     /gene="thpR"
                     /locus_tag="ACHUNN_003339"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0577634.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4825..7254
                     /gene="hrpB"
                     /locus_tag="ACHUNN_003340"
     CDS             4825..7254
                     /gene="hrpB"
                     /locus_tag="ACHUNN_003340"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0577635.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7450..9984
                     /gene="mrcB"
                     /locus_tag="ACHUNN_003341"
     CDS             7450..9984
                     /gene="mrcB"
                     /locus_tag="ACHUNN_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0577636.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
BASE COUNT         2340 a         2632 c         2878 g         2311 t
ORIGIN      
        1 cttgtatgta agacttcacg caacgtctcc ccatcaggaa acgccagctc cggtgcaatc
       61 tcaaacagcg gccacagcat aaatccacga ttcttcatat cgtagtgcgg aacggtcagg
      121 cgttcggtat ttatcacttc attaccaaac agcatgatat cgagatccag cgtgcgtggt
      181 ccccagcgtt cagctttacg aacacgaccc tgttgcagtt ctatgcgctg tgtatggttg
      241 agtaactctt ccggggcgag cgaagtttca agcgccacgg cggcgttgag gtaatcgggt
      301 tgatcttgcg gccccagcgg tggagtgcgg taaaacgaag aaacggcaag aatgtggctt
      361 tcagggatat cgcctaatgc tttcagggca gcattgacct gctccagcgg agaggccaga
      421 ttgctgccta tggcaatata cgccactgtc atgcggtacc ctcacgacgc ggtgcgcgtt
      481 tgcgtggacg acgagtacgg cgacgcggtg acggctcttc atccaactcg ttaagcatcc
      541 ctttttgatc tggtggcgcg gaaacctgga actcacccca ccatttcacc agacgctgca
      601 gttcagcgtt acgttcaact tcagctcgca aggccaacag gtcataagcc gcacggaact
      661 taggatgctc cagcagtttc catgcgcgtt taccctgacg tcgggacata cgcaactgca
      721 actgccagat atcgcgggtt aacgtcgtca ggcgtttcgg gattgccagt gaacggcagg
      781 cttcgtccag cacgtcgttc atcgccagcg cgaaagcgtc gtgataggtc aggccgcttt
      841 cctgggcgat cttctgtgcc gtctccagca gtgggtacca gaacatggcg gcaaacaaga
      901 acgccgggtt cacgcgcata tcgttatgga tacgcgtatc ggtattcttc agcacctgtt
      961 caatgatccg ctccatcggg ctgtcgccat tttccgtgaa gtagcgggta atggtcggga
     1021 acagcggctg gaacagatga tattcacaca acagcttata ggtttcgtaa ccgtagcccg
     1081 cttgtagcag tttaagcgat tcttcaaaca ggcgtgccgg tgggatatcg ttcagcaggg
     1141 tagcgaggcg agggatcggt tctgcggttt ccgggctgat gcgcataccc aatttggcgg
     1201 caaaacgtac cgcgcgcagc atccgtaccg gatcttcacg gtagcgcgtt tccgggttac
     1261 caatcagacg gataacgccg tccttcagat ccttcatacc gccaacgtaa tcacggacgg
     1321 taaaatccgc tacgctgtaa tagaggctgt tgatagtgaa atcgcggcgc tgggcgtctt
     1381 cttcgatgga gccgaaaatg ttgtcgcgca gcaacatgcc gttttgcccg cgttgggagg
     1441 tcgtgcggtc gctgacgtta ccttcgtggt gtccacggaa ggtcgcaact tcgataatct
     1501 ccgggccaaa cattacatga gccagacgga aacggcgacc caccaggcgg cagttacgga
     1561 acagtttgcg cacctgctca ggcgtggcgt tagtggttac gtcaaaatct ttcggctttt
     1621 tgccaagtaa caggtcgcgc acgccgccgc caaccagcca ggcttcgtat cccgctttgt
     1681 tgagcctgta cattaccttc agggcatttt cactgatatc tttgcgggaa atagcatgct
     1741 gctcacgcgg gatcaccgtc acctgtggac gggcgactgc ctgttcagcc tcgctttcct
     1801 cgcggcttag caccttgcgg caaaaattag cgactcgggt aaaaatagta cacctcggta
     1861 gtgtcaaaca tcattcagga caaaaaaata gcggctaatc atagctcagc atgacgcatt
     1921 tgagaatgtt gaatttacaa ttgccgactc gggcacggcg gtcagcgtcc agtttttgac
     1981 tgctgactga aggatttgtt cgacgctgaa atcctgccag tgtgcttctg cctgctgacc
     2041 cagaaattga agtgccgcga ttagtaccgg acgtggatcg cctttcggca acgcaggcgc
     2101 atgattctgc ttggaaagtt tagcgccttg tggattaagc gccagcggca gatgaatgta
     2161 atctggcact ttccagccaa aaagctggta cagcgagatt tgccttactg ttggttcaat
     2221 cagatcagcc ccacgcacta tttctgtaac gccctggaaa tgatcatcaa ccacaacagc
     2281 caggttgtag gcgaacaacc catcacggcg atgaatgata aaatcttccc gtgccagttt
     2341 ttcgtcggcg tgaataatgc cgcgcagctg gtcagtaaat tgcgtgaccg gatgctgctg
     2401 gcggatacgc actgcggcat tgtctggtcc atgatgcaac acccggcaat gaccgtcgta
     2461 aataccgcca atgctttgaa tacgcgcacg cgtacaggtg cagtaataac ttagtccttg
     2521 ttcatgtaac caggcgagtg cttcacgata ggcgtcgtga cgttgcgatt gccagagaac
     2581 atcgccgtcc cagtgcagac cgtaatgttc cagctggcgc aggatagttt ctgcggcacc
     2641 gggaacttca cgaggcgggt cgatatcttc tatgcgtacc agccagcgac cttgccgggc
     2701 gcgagcctgc aaatagctgc cgagcgcggc gatcagagag ccaaaatgaa gctcgccgga
     2761 aggagagggg gcgaagcggc caatatactg tgtgtctgtc atctctttga acaaaaaata
     2821 aggcgggagc atttcccgcc tgtggtaaac gtgatggaac ggctgtaatt agccagccat
     2881 ctgtttttcg cgaatttcag ccagcgtttt gcagtcgatg cacagatcgg ctgtcgggcg
     2941 cgcttccaga cggcgaatac caatttcaac accgcaggat tcgcagtagc cgaaatcttc
     3001 gtcttccact tttttcagcg tcttctcgat ctttttgatc agcttacgct cgcgatcgcg
     3061 gttacgcagt tcgaggctga actcttcttc ctgggctgca cggtctaccg ggtccgggaa
     3121 gttggctgct tcatcctgca tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt
     3181 acgccatgct tccagaatac gacggaagtg cgccagctgg gcttcattca tatactcttc
     3241 gcccggcttc tcctgatatg gttccacccc agcgatggcg agaatactca gggacgatgt
     3301 tttacggttt tgcccttctt gcatgttgct tctccttaac acgcactatc gatccccatg
     3361 ttcgggggaa aaatgaggcc gctataaata gcagatgctt ttccggatag caattatcta
     3421 aacgtaacac ttgacaactg tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa
     3481 attaaccagt tacttacttt actacaatgt aaccgacagt gattttttaa gagccatgcc
     3541 ttcagcagaa atttccgctt tgtaagccag aatttctacc cccctctgtt gagcttctga
     3601 caatagttgc gcgtatttct catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga
     3661 atgcagcacg gcgaaaaaga taaccgcacg ctggccttca gccgctacgt tcatcaactc
     3721 ccgaaggtgt ttctgacctc gttcagtgac cgcatcggga aaatatccct gttcgttctc
     3781 cgctaacgta accgatttca cttcaatata gcagtctgga cgcgaatccg cctgcaacat
     3841 aaagtcaata cggctgcgtt ctgcgccgta ttttacttcg cttttcagcg agctatagcc
     3901 tgacagttct gaaattgatt cattaaggat agcctctttc gtcaacctgt tagcccaaag
     3961 cgtattgacg caaataaatg cgccgctctg gctttgagtt agttcccagg tgtgtgggta
     4021 tttccgtttg gtgttgtctg aagtcgaata ccagacggta tcgccacgcg ttgcacaacc
     4081 ggtcatcgca cccgtattcg ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac
     4141 atcggctaaa aaacgtttgt aacgctgaat tagcgtcgcg cgctgtagag ggggagagaa
     4201 ttccattgcg acaatccttg ttattgcgtt agcgcccagc gttttagcgg cgtgtagcgt
     4261 gtgcgtccac gggcaaacga ggaggcgtaa agggtgaact ccgtcaccgc ataagaccag
     4321 ttaaaacctg gcggcgggat tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt
     4381 ggatgaaacg gacgattgct ttgaaaacaa ccgctgcggg cagcctgtga acggagcata
     4441 ttcgccagct ggattaagcc gcgtggcggc tgacgcatcc ctaaccacac cacacgcgaa
     4501 cgcagccatt gtccggcgtc atcaagcgtg agtgtgaaac caggttgacg aatccgtccg
     4561 gctaaaagag aaagcgcctt ctctttctcg gcgctcactt cgcctaaaaa tgccagagtc
     4621 agatgcaaat tatcggcggc gaccggacgt cccgcctcag gtgggaagtg tgtggcgcgc
     4681 cagtggataa tctgttcgcg gatttctgca ggtaagtcga tagcaaagaa cagacgttgc
     4741 ggttcagaca tgtgaggcac tcggttatga attaccgcga tgctacaatg tggcgcgaag
     4801 aatgttaacc ctctggagcg ttttgtgtcg tcgttgcccg ttgctgccgt cttacctgaa
     4861 ttacttaccg ctctcgattg tgcgccgcag gtattattaa gtgcgccgac cggggccggg
     4921 aaatcaacct ggctgccgct gcaactgctg gcgcatcccg gcattaacgg gaaaattatc
     4981 ctgctggagc cgcgtcgtct ggcggcacgt aacgtcgcgc aacggctggc ggagctgctt
     5041 aacgaaaagc caggcgatac cgttggctac cggatgcgtg cgcaaaactg cgtcgggcca
     5101 aatacccgtc tggaagtagt taccgaaggc gtgctgacgc ggatgatcca gcgtgacccg
     5161 gagttgagcg gtgttggatt ggtgatcctt gatgaatttc atgagcgcag cttgcaggcg
     5221 gatttggcgt tggcgctgtt actcgatgtg caacaaggtc tgcgtgatga ccttaaactg
     5281 ctgattatgt cggctacgct ggataacgac cgcttgcagc aaatgctgcc agaagcgccc
     5341 gtcgtcatct cagaagggcg ctcgtttccg gttgaacgcc attatttacc gctgcccgcg
     5401 catcagcgtt ttgacgaagc cgttgcggta gccaccgccg aaatgctgcg tcaggaaagc
     5461 ggatcattac tgttattttt acctggcgtc ggagaaattc agcgtgtgca ggaacaactg
     5521 gcttcgcgca tcggcagtga tgtgttgctc tgcccgctgt atggcgcgtt gtcgctgaac
     5581 gatcagcgaa aagcgatcct cccggcaccg caagggatgc gcaaagtggt gctggcgacc
     5641 aatattgctg aaaccagttt aaccattgaa ggtattcgtc tggtggtgga ttgtgcccag
     5701 gagcgtgtgg cgcgttttga gccgcgcacg gggcttacgc gactgattac tcaacgcgtt
     5761 agccaggcat ccatgacgca gcgtgccggg cgcgccgggc gtctggagcc gggtatctgc
     5821 ctgcatttaa tcgccaaaga acaagcagaa cgcgctgccg cgcaaagtga accggagatc
     5881 ttacaaagcg atctttccgg tttgctgatg gaattactgc aatggggatg cagcgatccg
     5941 gcgcagatga gctggctgga tcaaccgcca gtagtgaatc taatggccgc gaaacgtctg
     6001 ttacaaatgc tgggggcgct ggacggtgaa cggcttagtg cgcaagggca aaaaatggca
     6061 gcgctgggta acgatccgcg tttagcggca atgctggtta gcgcgaagaa cgacgacgaa
     6121 gctgctaccg cggcaaaaat tgccgccatt ctcgaagagc cgccactgat gggcaatagt
     6181 gacctgggcg tggcgttttc gcgcaatcaa ccagcctggc agcaacgtag tcagcaactg
     6241 ttaaaacgct taaacgtacg cggcggtgag gcagacagtt cgcttatcgc gccgctactt
     6301 gccggggcgt ttgccgatcg cattgctcat cgccgtgggc aagatggacg ctatcaactg
     6361 gcaaacggca tgggagcgat gctcgatgcc gacgacgcgc taagccgcca cgaatggttg
     6421 atcgcaccgt tattattgca gggcagcgcc tcgccggatg cgcggatttt actggcgcta
     6481 ccggtcgata ttgatgaatt agtacaacgc tgtccgcagc tggtgcagca atctgacacc
     6541 gtggaatggg atgacgcgca aggtacgctg aaagcctggc gtcggctaca aatcggtcag
     6601 ttgacggtga aagtgcagcc gctggcgaaa ccgtcagaag acgagttgca tcaggcgatg
     6661 cttaatggca tccgtgataa aggtttaagc gtgctcaact ggacggcgga agcggaacag
     6721 ctacgcttgc gtttgttatg cgccgcaaag tggttgccgg aatatgactg gccagcggtt
     6781 gatgatgaaa gtttattggc agcgctggaa acgtggctgc tgccacatat gacaggcgta
     6841 cattcactac gcggcctgaa atcactggac atttatcagg cactacgcgg attacttgat
     6901 tggggaatgc agcaacgtct ggatagtgaa ttgcctgcgc attacactgt gccgacggga
     6961 agccggatcg ccattcgtta tcatgaagat aacccgcccg cactggcggt gagaatgcag
     7021 gagatgtttg gtgaggccac taatccgact atcgcccagg ggggcgtgcc gctggtgctg
     7081 gagttgcttt cacccgccca aaggccacta caaatcacac gagatttgag cgccttctgg
     7141 aaaggagcgt accgtgaggt gcaaaaagag atgaaagggc gttatcccaa acacgtctgg
     7201 ccggacgacc cggcaaacac ggcaccgacg cgacggacga aaaagtattc gtaaggttgg
     7261 ttttctccct ctccctgtgg gagagggccg gggtgagggc atcagcgcgc acgttcaccc
     7321 tcaccctaac cctctccctc aagggagagg ggaccgatcg agcacaaatt ttgagagata
     7381 tcttcttctg tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg
     7441 agaaaaagca tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt
     7501 ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat
     7561 gatgactatg aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt
     7621 aagcctcgtg gcaaacgcgg ctggctatgg ctgctgttaa aactggctat cgtttttgcc
     7681 gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc
     7741 aaggtctggc aactgcccgc ggcagtttat ggccgaatgg tcaaccttga accagatatg
     7801 accatcagca agaacgaaat ggtgaagctg ctggaggcaa cacagtatcg tcaggtgtcg
     7861 aaaatgaccc gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt
     7921 ccgtttgatt tcccggacag caaagaaggg caggtgcgtg cgcgtctgac ctttgatggc
     7981 gatcatctgg cgacgatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt
     8041 gatccgcgcc tgatcaccat gatctcttcg ccaaacggtg agcagcgtct gtttgtgccg
     8101 cgcagtggtt tcccggactt gctggtggat actttgctgg cgacagaaga ccgtcatttc
     8161 tacgagcatg atgggatcag tctctactcc atcggacgtg cggtgctggc aaacctgacc
     8221 gccggacgca cggtgcaggg agcgagtacg ctgacgcaac agctggtgaa aaacctgttc
     8281 ctctccagcg agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg
     8341 gacgcgcgtt acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt
     8401 cagagcggcg acaacgaaat ccgcggcttc ccgcttgcga gcttgtatta ctttggtcgc
     8461 ccggtagaag agctcagcct cgaccagcag gcgctgttag tcggtatggt gaaaggggcg
     8521 tccatctaca acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg
     8581 cgtttgctac aacaacagca gattatcgat ggtgaactgt atgaaatgct cagcgcgcgt
     8641 cctctgggcg tacagccgcg cggtggggta atctctccac agccagcctt tatgcaactg
     8701 gtgcgtcagg agctgcaggc gaaattaggc gataaggtaa aagatctctc cggcgtgaag
     8761 atcttcacta cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc
     8821 attccggcac tgaagaaaca gcgtaagttg agcgatcttg aaactgcaat tgtggtcgtc
     8881 gaccgcttta gtggtgaagt tcgtgcgatg gtgggaggtt ctgagccgca gtttgcgggc
     8941 tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat
     9001 ctgacggctt taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgcca
     9061 atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat
     9121 agcgaaagcg gcagagtgat gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg
     9181 gtaaatctgg ggatggcgct ggggctgcct gcagttacgg agacctggat caaactgggc
     9241 gtgccgaaag atcagctgca tccggttccg gcgatgctgc tgggggcgtt gaacttaacg
     9301 ccaatcgaag tggcgcaggc attccagacc atcgccagcg gcggtaaccg tgcaccgctt
     9361 tctgcactgc gttcggtaat cgcggaagat ggcaaggtgc tgtatcagag cttcccgcag
     9421 gcagaacgcg ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg
     9481 gtacaacgtg gtacgggtcg tcaacttggg gcgaaatacc cgaacctgca tctggcaggg
     9541 aaaacaggga ctaccaacaa taacgtagat acctggtttg cgggcattga cggcagcacg
     9601 gtgaccatca cctgggtagg ccgtgataac aaccagccga ccaaactgta tggtgccagc
     9661 ggggcaatgt ctatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt
     9721 gttccgccag aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc
     9781 ggtggcatgc gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagc
     9841 gagatgcagc agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag
     9901 ccgcaacagc aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc
     9961 aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat accggatggc gaattgccat
    10021 ccggtaaaat aacatcccac ctaaagtatt aaccctttct tttcatctgg ttgtttatta
    10081 acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta
    10141 tcttatcttt ataataatca t
//
LOCUS       DAZOXR010000155        10143 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZOXR010000155 DAZOXR010000000
VERSION     DAZOXR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.159"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..611
                     /locus_tag="ACHUNN_003342"
                     /pseudo
     CDS             1..611
                     /locus_tag="ACHUNN_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /gene="prfH"
                     /locus_tag="ACHUNN_003343"
     CDS             608..1222
                     /gene="prfH"
                     /locus_tag="ACHUNN_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0577637.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHUNN_003344"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHUNN_003344"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0577638.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="ACHUNN_003345"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="ACHUNN_003345"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0577639.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="ACHUNN_003346"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="ACHUNN_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0577640.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="ACHUNN_003347"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="ACHUNN_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0577641.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHUNN_003348"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHUNN_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0577642.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="ACHUNN_003349"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="ACHUNN_003349"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0577643.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="ACHUNN_003350"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="ACHUNN_003350"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0577644.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="ACHUNN_003351"
     tRNA            9115..9190
                     /locus_tag="ACHUNN_003351"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="ACHUNN_003352"
     CDS             9796..9954
                     /locus_tag="ACHUNN_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577645.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2511 a         2456 c         2634 g         2542 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt aacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 ttt
//
LOCUS       DAZOXR010000156         9954 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZOXR010000156 DAZOXR010000000
VERSION     DAZOXR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.160"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="ACHUNN_003353"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="ACHUNN_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR0577646.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="ACHUNN_003354"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="ACHUNN_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR0577647.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="ACHUNN_003355"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="ACHUNN_003355"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0577648.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUNN_003356"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUNN_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="ACHUNN_003357"
     CDS             3565..3849
                     /locus_tag="ACHUNN_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0577649.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUNN_003358"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUNN_003358"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0577650.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUNN_003359"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUNN_003359"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0577651.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUNN_003360"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUNN_003360"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0577652.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUNN_003361"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUNN_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0577653.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2410 a         2607 c         2507 g         2430 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tatttaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     9901 aaataatatt atttcatcta tttttctcat tgtttttaat caccattcta tttt
//
LOCUS       DAZOXR010000157         9896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZOXR010000157 DAZOXR010000000
VERSION     DAZOXR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.161"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUNN_003362"
     CDS             complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUNN_003362"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0577654.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUNN_003363"
     CDS             1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUNN_003363"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0577655.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1905..2177
                     /locus_tag="ACHUNN_003364"
     CDS             1905..2177
                     /locus_tag="ACHUNN_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0577656.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUNN_003365"
     CDS             complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUNN_003365"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0577657.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUNN_003366"
     CDS             complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUNN_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0577658.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUNN_003367"
     CDS             complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUNN_003367"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0577659.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6190..6741
                     /locus_tag="ACHUNN_003368"
     CDS             6190..6741
                     /locus_tag="ACHUNN_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0577660.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6858..7901
                     /gene="selD"
                     /locus_tag="ACHUNN_003369"
     CDS             6858..7901
                     /gene="selD"
                     /locus_tag="ACHUNN_003369"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0577661.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7906..9867
                     /gene="topB"
                     /locus_tag="ACHUNN_003370"
     CDS             7906..9867
                     /gene="topB"
                     /locus_tag="ACHUNN_003370"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0577662.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2454 a         2472 c         2633 g         2337 t
ORIGIN      
        1 aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg atccttaact
       61 gatcggcatt acgaccgaag tcgctctttt tagatcacag tttcatctca acttatttgg
      121 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
      181 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
      241 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
      301 ctttcatttc gccttcagca gcggctttaa cggcagcttt gatctgctcg taagttgcag
      361 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
      421 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
      481 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
      541 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
      601 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
      661 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
      721 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
      781 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
      841 caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
      901 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
      961 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     1021 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     1081 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     1141 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     1201 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg
     1261 cttgaaacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     1321 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     1381 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     1441 tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     1501 cagttttacg tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat
     1561 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa
     1621 accaagtatg attccggctg tggttggccc agtttctacg aaccggtgag tgaagaatcc
     1681 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     1741 tgtgatgccc atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat
     1801 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     1861 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     1921 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     1981 aatggcccga tggcgttgcg ttgaccgagg aacaaaaagc gaactgcctg caactggtga
     2041 tgctgtggca agctcgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     2101 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     2161 ttgctatgtt taagtgaatg agttatctag tatttaagcc tgttattaag ggagggcaaa
     2221 tatacttgtt aggacgtcgt gttttagctg caagtaggtt aaatgcaaca ctgacaagta
     2281 ttatctggcc tacaatgtca ttgaggaaat gtaggccaga gatgaattca actaatatta
     2341 cccctgtgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
     2401 cataaacgcg tgcgcactgt cctggggctg gatattcacg ccacgacaac catcggtaat
     2461 cacatttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
     2521 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgtaacc agtcatcgag
     2581 cgaggttttc tgccgacggc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
     2641 ttcgccttta tggaacaccg ctgcgatcgc tttttggttc agtaacggat gtaattgtgc
     2701 gccttcactg ttctgcacac agtgatctgg ccagaaggtt tgtggcaaac cgtcgagttg
     2761 gcctggcgta taaggctcta caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg
     2821 ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt
     2881 agcgacatcc accgtactgt caccttccgg cacggcgagc gcgccaccag cacagaaatc
     2941 attttgtaaa tcgaccagta acagggcgcg agggggcatt acagtctcct taatcatccg
     3001 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
     3061 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgc
     3121 caataacgcc ggcatgggcg agggcgttac cggtggcata accacccata ttcactttac
     3181 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
     3241 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
     3301 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
     3361 tcactacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
     3421 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
     3481 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
     3541 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
     3601 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
     3661 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcagtat
     3721 acgccatcgt gtcggtgccg tgcaggatga caaaaccatc atagtcgtca tagtgcgctt
     3781 taatatcttc agcaatatgc tgccagtctt ctggcgtcat atcagaagaa tccatcagcg
     3841 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
     3901 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
     3961 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
     4021 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
     4081 gataaggaaa tatggggcga gacagcggga agaggattcg tcgacgtaaa aaataatgtg
     4141 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
     4201 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
     4261 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
     4321 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
     4381 tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc gaccgcatca
     4441 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
     4501 tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc agcaaccagc
     4561 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
     4621 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
     4681 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
     4741 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
     4801 ccagatgccg ccatgccgcc catcgataca accacaggct tacccgctgc ccgggctgct
     4861 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
     4921 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
     4981 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
     5041 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
     5101 gcgcgataat ttttatcagt cttactccag ccgaactctt tggttagagc tttttcgatt
     5161 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
     5221 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
     5281 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
     5341 cggctgtcag cttcgcgggc tgccggtgac atgtcatcac gaataaacgg ttcaacggca
     5401 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
     5461 ttgtagtaca gaccgttggt agcaaaaccg tgcaaatcca ccacgccttg cggtgacagc
     5521 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
     5581 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
     5641 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
     5701 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
     5761 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
     5821 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
     5881 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
     5941 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
     6001 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
     6061 caggcgcaat agatgtggtt atcgtaatga cacaaccggc acttgtcagc aggaattgcg
     6121 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
     6181 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
     6241 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
     6301 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gcgcgagcgt
     6361 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgacaa agctatcgac
     6421 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcgaa
     6481 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
     6541 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
     6601 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     6661 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
     6721 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     6781 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
     6841 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
     6901 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     6961 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgagac ccgcgacgat gcggcggtgt
     7021 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     7081 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
     7141 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     7201 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     7261 cgctggctgg cggtcactcc atcgatgcga cggagccgat ttttggtctg gcggtaacgg
     7321 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
     7381 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgctga
     7441 aaccagaaca tcagggacta gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
     7501 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt ggtctgctgg
     7561 gccatttgag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgttgac tatgacgcga
     7621 tcccaaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg
     7681 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatt
     7741 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
     7801 atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcaatt ggcgaactgg
     7861 tgccagcgcg cggcggtcgt gccatggttg agattcgtta attcaatgcg gttgtttatt
     7921 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
     7981 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat cggtcacctg
     8041 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
     8101 ttgccgatta tcccggaaaa gtggcaatta cagccccgac cctccgtgac caaacaactt
     8161 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
     8221 cgtgaagggc aactgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
     8281 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
     8341 ctcgaccgtc ttcggtccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
     8401 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
     8461 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
     8521 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
     8581 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
     8641 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
     8701 gttaaccgca ttagtggtca accagcaatt gtcaccagct ataacgataa acgggaatca
     8761 gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttt
     8821 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
     8881 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
     8941 gcggtgatga atgccatcag cgttcatgca ccagatctgt tgctgcagcc agtggtagat
     9001 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcacacca cgccatcatc
     9061 ccgaccgcac ggagttcagc catcaacctg acggagaacg aagcgaaggt ctataacctg
     9121 attgcccgtc agtacttgat gcagttctgc ccggatgtgg tgttccgcaa gtgtgttatc
     9181 gaactggaca ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc tgaagcaggc
     9241 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac gccattgcct
     9301 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
     9361 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
     9421 tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa
     9481 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
     9541 cgctatatcc actctaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
     9601 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
     9661 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
     9721 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
     9781 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
     9841 gaagtcggaa gcggggcgat agcgtaagcg agtgaatctt tcgtgctatt cgagtc
//
LOCUS       DAZOXR010000158         9851 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZOXR010000158 DAZOXR010000000
VERSION     DAZOXR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.162"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHUNN_003371"
     CDS             complement(<1..163)
                     /locus_tag="ACHUNN_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0577663.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUNN_003372"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUNN_003372"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0577664.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUNN_003373"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUNN_003373"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0577665.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUNN_003374"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUNN_003374"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0577666.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUNN_003375"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUNN_003375"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0577667.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUNN_003376"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUNN_003376"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0577668.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHUNN_003377"
     CDS             5058..6191
                     /locus_tag="ACHUNN_003377"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0577669.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHUNN_003378"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHUNN_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0577670.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUNN_003379"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUNN_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0577671.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUNN_003380"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUNN_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0577672.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUNN_003381"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUNN_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0577673.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUNN_003382"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUNN_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0577674.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUNN_003383"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUNN_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0577675.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9851
                     /gene="yjeJ"
                     /locus_tag="ACHUNN_003384"
     CDS             9762..>9851
                     /gene="yjeJ"
                     /locus_tag="ACHUNN_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0577676.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2505 c         2502 g         2327 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg c
//
LOCUS       DAZOXR010000159         9754 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZOXR010000159 DAZOXR010000000
VERSION     DAZOXR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.163"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUNN_003385"
     CDS             <1..347
                     /locus_tag="ACHUNN_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0577677.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUNN_003386"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUNN_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR0577678.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUNN_003387"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUNN_003387"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0577679.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHUNN_003388"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHUNN_003388"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0577680.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHUNN_003389"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHUNN_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0577681.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHUNN_003390"
     CDS             complement(4344..5309)
                     /locus_tag="ACHUNN_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0577682.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHUNN_003391"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHUNN_003391"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0577683.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHUNN_003392"
     CDS             6366..6746
                     /locus_tag="ACHUNN_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0577684.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHUNN_003393"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHUNN_003393"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0577685.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHUNN_003394"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHUNN_003394"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0577686.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2249 a         2472 c         2545 g         2488 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaac
//
LOCUS       DAZOXR010000160         9644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZOXR010000160 DAZOXR010000000
VERSION     DAZOXR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.164"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUNN_003395"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUNN_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0577687.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUNN_003396"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUNN_003396"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0577688.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUNN_003397"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUNN_003397"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0577689.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHUNN_003398"
     CDS             complement(5698..7014)
                     /locus_tag="ACHUNN_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR0577690.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHUNN_003399"
     CDS             complement(7004..8761)
                     /locus_tag="ACHUNN_003399"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0577691.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9644)
                     /locus_tag="ACHUNN_003400"
                     /pseudo
     CDS             complement(8777..9644)
                     /locus_tag="ACHUNN_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DAZOXR010000161         9623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZOXR010000161 DAZOXR010000000
VERSION     DAZOXR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.165"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..2200
                     /locus_tag="ACHUNN_003401"
                     /pseudo
     CDS             1..2200
                     /locus_tag="ACHUNN_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2193..3329
                     /locus_tag="ACHUNN_003402"
     CDS             2193..3329
                     /locus_tag="ACHUNN_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0577692.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3326..5326
                     /locus_tag="ACHUNN_003403"
     CDS             3326..5326
                     /locus_tag="ACHUNN_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR0577693.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5448..5909
                     /locus_tag="ACHUNN_003404"
     CDS             5448..5909
                     /locus_tag="ACHUNN_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0577694.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5950..6420)
                     /locus_tag="ACHUNN_003405"
     CDS             complement(5950..6420)
                     /locus_tag="ACHUNN_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0577695.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6467..7186)
                     /gene="btsR"
                     /locus_tag="ACHUNN_003406"
                     /gene_synonym="yehT"
     CDS             complement(6467..7186)
                     /gene="btsR"
                     /locus_tag="ACHUNN_003406"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0577696.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7183..8868)
                     /gene="btsS"
                     /locus_tag="ACHUNN_003407"
     CDS             complement(7183..8868)
                     /gene="btsS"
                     /locus_tag="ACHUNN_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0577697.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9090..9155
                     /locus_tag="ACHUNN_003408"
                     /pseudo
     CDS             9090..9155
                     /locus_tag="ACHUNN_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9383..>9623
                     /locus_tag="ACHUNN_003409"
     CDS             9383..>9623
                     /locus_tag="ACHUNN_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0577698.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2354 a         2531 c         2470 g         2268 t
ORIGIN      
        1 gcggccacga tacgtattga ttgaaggccc ggctgatttt aatgaccggg tagacgaact
       61 gtttttagcc caccagcttc cggtagctat ttacagttat tgccagtatc aggacggtgc
      121 agcccccggg cgtggtgcct ggacgccatt tgctgaattt tcgccggagt ggcaggcgct
      181 acaagccgca cgtcgtattc aggcacaaac ttacttcatc gatttgcctt gctgggcgca
      241 gagtgaagaa gtggacgatt cgcctgatac gcaagaggaa agccaggcct tgctgctgcg
      301 tgccacccgc atggataaca gcgacaccct gtgggatcac ttgttcgaag atgaaagcca
      361 gcaaactgca ttaccctctg cactggcgca ctattttgcc caactgcggg gcgattcccc
      421 cggcgatgca ctcaatcgtc agcgcgaagc ctttatggcc cgctggattg gatgggcgat
      481 gcagcaaaac aatggcgacg tgttagtcgt ctgcggtggc tggcacgctc ctgcactggc
      541 aaaaatgtga cgcgaatgcc cgcaggacat taacacgccg gaattgccct cgctggcaga
      601 tgccgttaca ggttgttatc tcacccccta cagtgaaaag cgccttgatg tgctggcagg
      661 atacctttcc ggtatgcctg ccccggtctg gcaaagctgg tactggcagt ggggcttaca
      721 gcaggcggga gaacaactgc taaaaacggt tcttacccgt ttgcgccagc acaaattgcc
      781 tgcttcgacc gcggatatgg ctgccgctca tctgcatgcg atggcgctgg cacagttgcg
      841 cggacataaa ctaccgttac gcactgactg gctggatgcc atagcaggct cgctgattaa
      901 agaagccctg aacgcgccgt tgccgtggag ctatcgcgga gttattcatc ccgacaccga
      961 tccgattctg ctaacgttga tagacacatt agcgggtgac ggattcggta aacttgcccc
     1021 ttctacgcca caaccgcctc tgccaaaaga tgtcacctgc gaactggaac gtaccgcaat
     1081 ctctcttccg gcggagctta ccttaaatcg ctttaacccc aatgggctag cgcaaagtca
     1141 ggtgttacat cggctggcaa tactggagat cccagggatt gtacgccagc agggaagcac
     1201 actgacactt gcaggcaacg gtgaagaacg ctggaaatta acccgcccgc ttagccagca
     1261 tgcggcattg attgaggccg cctgctttgg tgccacactc caggaagccg cacgccataa
     1321 attagaagcc gatatgctgg acgcgggtgg aatcggcagt atcaccacat gtcttagcca
     1381 ggcggcgtta gcgggtctgg cgtccttcag tcaacaatta ctggagcaac tcacattatt
     1441 aatcgcccag gaaaatcaat ttgccgaaat gggccaggcg ctggaagtgc tatatgcctt
     1501 atggcggctg gatgaaatta gcggtatgca aggcgcgcag atattacaaa cgacgttatg
     1561 cgcggctatc gatcgcacgc tgtggctgtg tgaatctaac ggcagaccag atgaaaagga
     1621 gtttcacgct cacctgcata gctggcaggc gctttgccat atcctgcgcg atctacatag
     1681 cggcgttaat ttacccggcg tttttctttc tgcggcggta gccttactgg agcgccgcag
     1741 tcaggcgatt catgtcccgg cgctggatcg cggcgcggct cttggcgcac tgatgcgtct
     1801 ggaacatccc aacgccagtg ccgaagcggc gctgacgatg ctggcgcagt tatccccggc
     1861 acaatccggc gaggcgctgc acggtttgct ggcgctcgcc cgtcatcaac tggcctgcca
     1921 accagcattt atcgccggtt tcagtagtca tttaaatcaa ctgagtgacg acgattttat
     1981 taacgccctg cccgatttgc gcgcggcgat ggcctggcta ccgccacgag aacgcgggac
     2041 actggcgcat caggtgcttg agcattacca gcttgcgcag cttcctgttt cagcgctgca
     2101 aatgccgttg cattgtcctc cgcaagccat tgcacatcat caacaactcg aacagcaggc
     2161 actggcatcg ctgcaaaact ggggagtttt ccatgtctga actgaacgat cttctgacca
     2221 cccgtgagct acaacgctgg cgattaattc ttggcgaagc ggcagaaacg acgctttgtg
     2281 ggctggatga caacgcccgg cagatagacc acgcgctgga atggctgtat gggcgcgatc
     2341 ctgaacggct ccagcgtggt gaacgctccg gtggattagg tggctcaaat ctcaccactc
     2401 ctgagtggat caacagtatt cacacgctgt ttccgcaaca ggtgattgag cggctggaaa
     2461 gcgatgccgt actgcgctac ggcattgaag atgtggtgac gaatctcgac atgctggaac
     2521 gtatgcagcc ttctgaaagc ctgctacgcg ccgttttgca caccaaacat ctgatgaatc
     2581 ccgaagtact ggctgccgcc cgccggatag tgcgccaggt tgttgaagaa attatggctc
     2641 gactggcaaa ggaagttcgt caggcttttt ctggtgtccg cgatcgccgt cgccgctcat
     2701 ttattccact ggcgcgaaac tttgatttca aaagtactct gcgcgctaac ctgcaacact
     2761 ggcacccgca acacggcaag ttgtatatcg aatccccccg ctttaacagc cgcattaaac
     2821 gccaaagcga acaatggcaa ctggtcttac tggttgatca aagcggatcg atggtggact
     2881 cggtgatcca ctctgcggtg atggcggcct gtttgtggca gttacccggc attcgtaccc
     2941 atctggtggc gtttgacaca agcgtggttg atctcacggc agacgttgcc gatccggtag
     3001 agttattaat gaaagtacag ttgggcggcg ggaccaatat cgccagtgcc gtggagtatg
     3061 gtcggcaact tattgaacaa ccagcaaaaa gcgtcattat cctcgtgagc gatttttacg
     3121 aagggggttc atcatcattg ctgacgcatc aggtgaaaaa gtgtgtccag agcggcatca
     3181 aagtgctggg actggctgcg ctcgatagca ccgcaacacc ttgctatgac cgcgatacgg
     3241 cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat gacgccgggc gagctggcat
     3301 catggcttgc ggagaatctt cagtcatgaa ttcactacgt ccggaattat tagaactgac
     3361 accgcaggcc ctgacggcgt taagcaatgc cggttttgtt aagcgcagtc ttaaggaact
     3421 ggaaaatggc aacgtcccgg agatcagcca tgagaacgac gctttaatcg ccaccttcag
     3481 tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg aaagaggctc agtgcagttg
     3541 cggggccaac ggtatgtgcc gtcatcgcgt gatgctggtg ttaagttatc aacgactttg
     3601 tgccaccact cagtctacgg aaaaagaaga agagtgggat ccggcaatct ggctggaaga
     3661 actggctacc cttcccgatg ctacccgcaa acgcgcacag gcgctggtcg ctaaaggcat
     3721 caccattgag ttgttctgtg cgccgggtga aattccctct gcccgcttac cgatgagcga
     3781 tatgcgtttt tattcccgca gcagtattcg tttcgcccgt tgtgattgta ttgaaagcac
     3841 actttgcgaa catgtcgtac tggcagtaca ggcctttgtc caggcaaaag cgcagcaagc
     3901 ggaattgact catttaatct ggcagatgcg cagcgaacac gtcacatcat ctgacgatcc
     3961 gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt caacaactaa gccaggcatt
     4021 atggctgagc ggcatcagcc agccgcttat tcactacgag gccgctttca gccgcgcgca
     4081 gcaagcagcg gaacgctgca gctggcgatg ggtgagtgaa tcgctacggc aactgcgcgc
     4141 cagcgttgat gccttccacg cccgcgccag ccactatcat gccggagaat gcttacgtca
     4201 gcttgcggca ttaaacagtc gattaaattg cgcacaagag atggcccggc gcgacagtat
     4261 tggtgaagtt cctcctgtgc cgtggcgcac ggtcgttggc tctggcattg ccggagaagc
     4321 aaagcttgat catctgcggc tggtgtcttt aggtatgcgt tgctggcagg atattgagca
     4381 ttatggttta cgcatctggt ttaccgatcc cgacaccggc agtattttgc acctttcgcg
     4441 cagttggccg cgaagtgaac aggaaaactc accggcagct acgcgtcggc tgtttagttt
     4501 tcaggctggc gcactagcgg gcgggcaaat tgtttcacaa gcagcaaaac gcagtgccga
     4561 tggcgagctg ctgttagcta cccgcaaccg cttaagcagc gttgtgccgc tgtcgcctga
     4621 tgcctggcaa atgttgagcg cgccgttacg ccagccgggc attgtggctt tgcgggaata
     4681 tttacgccag cgtccccccg cctgcatacg gcctcttaat caggtcgata acttatttat
     4741 tctgccggtc gctgagtgta tttcgctcgg ttgggatagc agccgccaga cgctggatgc
     4801 gcaggtaatc agcggcgaag gtgaagataa tctcctgacg ttatcccttc cggcatctgc
     4861 ctgttctcct tttgccgttg aacgcatggc ggcgcttttg caacaaacag acgaccccgt
     4921 gagtctggtt tctggctttg tcagctttgt agaggggcaa ttgacactgg aaccacgggt
     4981 gatgatgaca aaaacccgcg cctgggcgct ggacgcagaa actgcgcctg tggcaccgct
     5041 accttctgcc agcgttttgc ctgtgccgtc taccgctcat cagttgctga tgcgctgcca
     5101 ggcgttactt attcaactgc tccataacgg ctggcgctat caggaacaga gcgctattag
     5161 tcaggcagag ttgctggcga atgacctctc cgcggtcggt ttttatcggc tggcacatgt
     5221 gttgggacaa tttcgtaata cagaaagcga ggcacgggta gaagcaatga ataacggtgt
     5281 tttactttgc gaacaattat tccccatgct tcagcaacaa ggatgaaata gtgcttacta
     5341 agagttctac tccagttccg gactgctcac gccacggtat taggcatatc ctatatagcc
     5401 cctggtgaga gtcaccagtt ccttgattaa ataaaatgga gttttacatg aaggctttca
     5461 ataagctgtt ttccctcgtt gttgcatctg ttctggtttt ctctcttgct ggctgcggtg
     5521 acaaagaaga atcgaagaaa ttcagcgcca atctgaacgg cactgaaatt gccattacct
     5581 atgtctacaa aggtgacaag gtgcttaagc aatcttctga aaccaaaatt caatttgcct
     5641 ccattggtgc aaccaccaaa gaagatgctg ccaagacact tgagccgtta agcgccaaat
     5701 acaaaaacat cgcgggtgtt gaagaaaaat taacctatac cgatacctac gcgcaggaaa
     5761 acgtgactat cgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca
     5821 acgtttctgc tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga
     5881 aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc
     5941 atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg
     6001 taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca tcatcgctgt
     6061 aatttccggc atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt
     6121 tttcagcgaa aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc
     6181 cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag
     6241 gaatgacgac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag
     6301 ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag
     6361 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat
     6421 gaggccttca ggtgttaatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
     6481 cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
     6541 tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca
     6601 gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt
     6661 cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga
     6721 caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa
     6781 atttcagcgc ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac
     6841 gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
     6901 aggcatgttc ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga aaaacaatat
     6961 acgggcgatg ttccgggtca agcatcccca ccatttccag accactgatg cgcggcatct
     7021 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg
     7081 cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acacgcaggt
     7141 tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
     7201 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata
     7261 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
     7321 attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc
     7381 ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt
     7441 aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc
     7501 ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag
     7561 ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa
     7621 gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg
     7681 gcggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatgaggat tcacctgggc
     7741 gtgaagcagt ttgatttctg actgggtgag catcgctttt tgccgctcat attgcccggc
     7801 aagaatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt ttattgaact
     7861 gaataaacgg tttttggctt catacaattt gatggtgccc atcacgcgct gattttcgcc
     7921 acgcaacgga attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata
     7981 aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta
     8041 aggcgaagaa atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag
     8101 caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg
     8161 agccactttc atgctgttca cttcgttaaa cccctgtcgc aaaatgcctt ccgtcgaggc
     8221 tgccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt
     8281 atcgagcaat atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggcgc
     8341 agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat
     8401 cagcatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac
     8461 tttatcagtg cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc
     8521 aacgatggtt gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa
     8581 gccgccggtc agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac
     8641 cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc
     8701 catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt
     8761 gacctgcatt aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa
     8821 aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca
     8881 cacttccctt taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc
     8941 agaaaaatga gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc
     9001 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
     9061 accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
     9121 tgctttgtga tattaaccct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
     9181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
     9241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
     9301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
     9361 accacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
     9421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
     9481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
     9541 acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg
     9601 ggagtctgct gacgagtggt ttg
//
LOCUS       DAZOXR010000162         9542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZOXR010000162 DAZOXR010000000
VERSION     DAZOXR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.167"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1321
                     /locus_tag="ACHUNN_003410"
     CDS             <1..1321
                     /locus_tag="ACHUNN_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0577699.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHUNN_003411"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHUNN_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR0577700.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHUNN_003412"
     CDS             2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHUNN_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR0577701.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="ACHUNN_003413"
     CDS             3479..6418
                     /locus_tag="ACHUNN_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR0577702.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="ACHUNN_003414"
     CDS             6351..6665
                     /locus_tag="ACHUNN_003414"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0577703.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHUNN_003415"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHUNN_003415"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="ACHUNN_003416"
     CDS             complement(7120..7608)
                     /locus_tag="ACHUNN_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0577704.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHUNN_003417"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHUNN_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR0577705.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAZOXR010000163         9501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZOXR010000163 DAZOXR010000000
VERSION     DAZOXR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.169"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUNN_003418"
     CDS             <1..108
                     /locus_tag="ACHUNN_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0577706.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHUNN_003419"
     CDS             complement(149..1231)
                     /locus_tag="ACHUNN_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR0577707.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHUNN_003420"
     CDS             complement(1228..3060)
                     /locus_tag="ACHUNN_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0577708.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHUNN_003421"
     CDS             complement(3275..3631)
                     /locus_tag="ACHUNN_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577709.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHUNN_003422"
     CDS             complement(3689..4111)
                     /locus_tag="ACHUNN_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0577710.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHUNN_003423"
     CDS             complement(4127..4852)
                     /locus_tag="ACHUNN_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR0577711.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHUNN_003424"
     CDS             complement(4874..5620)
                     /locus_tag="ACHUNN_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0577712.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHUNN_003425"
     CDS             complement(5627..6691)
                     /locus_tag="ACHUNN_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR0577713.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGGPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHUNN_003426"
     CDS             complement(6763..7188)
                     /locus_tag="ACHUNN_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0577714.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHUNN_003427"
     CDS             complement(7185..7448)
                     /locus_tag="ACHUNN_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0577715.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHUNN_003428"
     CDS             7560..8060
                     /locus_tag="ACHUNN_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0577716.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHUNN_003429"
     CDS             8080..8274
                     /locus_tag="ACHUNN_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR0577717.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHUNN_003430"
     CDS             8274..8564
                     /locus_tag="ACHUNN_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0577718.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHUNN_003431"
     CDS             8844..8996
                     /locus_tag="ACHUNN_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0577719.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHUNN_003432"
     CDS             9008..9373
                     /locus_tag="ACHUNN_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577720.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2494 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggcc ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAZOXR010000164         9432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZOXR010000164 DAZOXR010000000
VERSION     DAZOXR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.170"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHUNN_003433"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHUNN_003433"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0577721.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUNN_003434"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUNN_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0577722.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHUNN_003435"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="ACHUNN_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUNN_003436"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUNN_003436"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0577723.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUNN_003437"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUNN_003437"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0577724.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUNN_003438"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUNN_003438"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0577725.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHUNN_003439"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHUNN_003439"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0577726.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHUNN_003440"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHUNN_003440"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0577727.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHUNN_003441"
     CDS             complement(6556..7449)
                     /locus_tag="ACHUNN_003441"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0577728.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHUNN_003442"
     CDS             complement(7446..8099)
                     /locus_tag="ACHUNN_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0577729.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHUNN_003443"
     CDS             complement(8110..9369)
                     /locus_tag="ACHUNN_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0577730.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAZOXR010000165         9419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZOXR010000165 DAZOXR010000000
VERSION     DAZOXR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.171"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..168
                     /locus_tag="ACHUNN_003444"
     CDS             <1..168
                     /locus_tag="ACHUNN_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0577731.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="ACHUNN_003445"
     CDS             complement(340..669)
                     /locus_tag="ACHUNN_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0577732.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="ACHUNN_003446"
     CDS             complement(674..859)
                     /locus_tag="ACHUNN_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0577733.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="ACHUNN_003447"
     CDS             complement(856..3495)
                     /locus_tag="ACHUNN_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0577734.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="ACHUNN_003448"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="ACHUNN_003448"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0577735.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="ACHUNN_003449"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="ACHUNN_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0577736.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="ACHUNN_003450"
     CDS             complement(5222..5488)
                     /locus_tag="ACHUNN_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0577737.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="ACHUNN_003451"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="ACHUNN_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0577738.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2343 a         2460 c         2304 g         2312 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcat
//
LOCUS       DAZOXR010000166         9188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZOXR010000166 DAZOXR010000000
VERSION     DAZOXR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.172"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUNN_003452"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUNN_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHUNN_003453"
     CDS             complement(1751..2509)
                     /locus_tag="ACHUNN_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0577739.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUNN_003454"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUNN_003454"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0577740.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUNN_003455"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUNN_003455"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0577741.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUNN_003456"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUNN_003456"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0577742.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHUNN_003457"
     CDS             complement(5474..6079)
                     /locus_tag="ACHUNN_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577743.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="ACHUNN_003458"
     CDS             complement(6082..6849)
                     /locus_tag="ACHUNN_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577744.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="ACHUNN_003459"
     CDS             complement(6852..7562)
                     /locus_tag="ACHUNN_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577745.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="ACHUNN_003460"
     CDS             complement(7578..9083)
                     /locus_tag="ACHUNN_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0577746.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2262 g         2196 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAZOXR010000167         9072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZOXR010000167 DAZOXR010000000
VERSION     DAZOXR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.173"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHUNN_003461"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHUNN_003461"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR0577747.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHUNN_003462"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHUNN_003462"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR0577748.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHUNN_003463"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHUNN_003463"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR0577749.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHUNN_003464"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHUNN_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR0577750.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHUNN_003465"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHUNN_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR0577751.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHUNN_003466"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHUNN_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR0577752.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHUNN_003467"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHUNN_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR0577753.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHUNN_003468"
     CDS             8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHUNN_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR0577754.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8343..9040
                     /locus_tag="ACHUNN_003469"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="ACHUNN_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1987 a         2508 c         2585 g         1992 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DAZOXR010000168         8999 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZOXR010000168 DAZOXR010000000
VERSION     DAZOXR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.174"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            137..1535
                     /gene="ulaA"
                     /locus_tag="ACHUNN_003470"
                     /pseudo
     CDS             137..1535
                     /gene="ulaA"
                     /locus_tag="ACHUNN_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1551..1856
                     /gene="ulaB"
                     /locus_tag="ACHUNN_003471"
     CDS             1551..1856
                     /gene="ulaB"
                     /locus_tag="ACHUNN_003471"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0577755.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1866..2330
                     /gene="ulaC"
                     /locus_tag="ACHUNN_003472"
     CDS             1866..2330
                     /gene="ulaC"
                     /locus_tag="ACHUNN_003472"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0577756.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2344..2994
                     /gene="ulaD"
                     /locus_tag="ACHUNN_003473"
     CDS             2344..2994
                     /gene="ulaD"
                     /locus_tag="ACHUNN_003473"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0577757.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3004..3858
                     /gene="ulaE"
                     /locus_tag="ACHUNN_003474"
     CDS             3004..3858
                     /gene="ulaE"
                     /locus_tag="ACHUNN_003474"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0577758.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3858..4544
                     /gene="ulaF"
                     /locus_tag="ACHUNN_003475"
     CDS             3858..4544
                     /gene="ulaF"
                     /locus_tag="ACHUNN_003475"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0577759.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4641..5192
                     /locus_tag="ACHUNN_003476"
     CDS             4641..5192
                     /locus_tag="ACHUNN_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR0577760.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5267..5582)
                     /locus_tag="ACHUNN_003477"
                     /pseudo
     CDS             complement(5267..5582)
                     /locus_tag="ACHUNN_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            5909..6304
                     /gene="rpsF"
                     /locus_tag="ACHUNN_003478"
     CDS             5909..6304
                     /gene="rpsF"
                     /locus_tag="ACHUNN_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0577761.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6311..6625
                     /gene="priB"
                     /locus_tag="ACHUNN_003479"
     CDS             6311..6625
                     /gene="priB"
                     /locus_tag="ACHUNN_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0577762.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6630..6857
                     /gene="rpsR"
                     /locus_tag="ACHUNN_003480"
     CDS             6630..6857
                     /gene="rpsR"
                     /locus_tag="ACHUNN_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0577763.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6899..7348
                     /gene="rplI"
                     /locus_tag="ACHUNN_003481"
     CDS             6899..7348
                     /gene="rplI"
                     /locus_tag="ACHUNN_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0577764.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7460..8431)
                     /locus_tag="ACHUNN_003482"
     CDS             complement(7460..8431)
                     /locus_tag="ACHUNN_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0577765.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8484..8900)
                     /locus_tag="ACHUNN_003483"
                     /pseudo
     CDS             complement(8484..8900)
                     /locus_tag="ACHUNN_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(8917..>8999)
                     /locus_tag="ACHUNN_003484"
     CDS             complement(8917..>8999)
                     /locus_tag="ACHUNN_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0577766.1"
                     /translation="KSLSFSKSVELHDKVIGYYLNIKHYQ"
BASE COUNT         2168 a         2199 c         2386 g         2246 t
ORIGIN      
        1 ccgtgtggaa ttatttgcgg gtcgcgtcac atttaatcat aaataatctt gttgtgatta
       61 cttttgaaaa ttagagtgag tgcacaacat tccgggtgtg cggaataccc ggttacctct
      121 tcttcaggag atcgttatgg agatcctcta caacatcttt accgtgtttt ttaaccaggt
      181 catgaccaat gccccgttgt tgctgggtat tgtgacctgt ctgggctaca tcctactgcg
      241 caaaagtgtc agcgttatta ttaaaggcac gattaaaacc ataattggtt tcatgttgtt
      301 gcaggcaggg tccggcatcc tcaccagcac cttcaaaccg gtggtggcga aaatgtccga
      361 agtctacggc attaaacggc gcaatttccg atacttacgc gtcgatgatg gcaaccatcg
      421 accgcatggg cgatgcctat agctgggtag gttacgcggt gttgttagcg ctggcgctga
      481 acatctgtta cgtgctgctg cgtcgcatta ccggtattcg taccatcatg ctgactggtc
      541 atatcatgtt ccagcaggcg gggctgattg ccgttacgtt gtttattttc ggctactcca
      601 tgtggacaac cattatctgc acggcgattc tggtttcgct ctactggggc attacctcca
      661 acatgatgta caagccgact caggaagtga cggacggctg cggtttctcc atcggtcacc
      721 agcaacagtt tgcatcatgg actgcctata aagttgcgcc gttcctcggc aaaaaagagg
      781 aaagcgttga agacctcaaa ctgccaggct ggctgaatat tttccacgac aacatcgtct
      841 ccacggcgat tgtcatgacc atcttctttg gtgccattct gctctccttc ggtatcgaca
      901 ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta tatcctgcaa accggttttt
      961 ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg catgtttgtg gcggaactct
     1021 ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg tgcggttctg gcgattgact
     1081 gtgcggctat ttatagcttc gcgccgaacg ccgtggtctg gggctttatg tggggcacca
     1141 tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg ttcctcgagc ctgattatcc
     1201 caggctttat cccgatgttc ttctccaacg ccaccatcgg cgtgttcgct aaccacttcg
     1261 gcggctggcg tgcggcgctg aagatttgtc tggtgatggg gatgatcgaa atctttggtt
     1321 gcgtctgggc ggtgaaactt accggtatga gtgcctggat ggggatggcg gactggtcga
     1381 ttctggcacc gccgatgatg cagggcttct tctccatcgg tatcgccttt atggccgtca
     1441 tcattgtaat tgcactggct tatatgttct tcgctggccg cgcactgcgc gcagaagaag
     1501 atgcagaaaa acaactggca gaacagtctg cttaataagg agttttgatt atgaccgtac
     1561 gtattctggc tgtgtgtggc aacggacaag gcagttccat gatcatgagg atgaaagtgg
     1621 accagttttt aacccaatca aacattgacc acacggtaaa tagctgcgcg gttggcgaat
     1681 acaaaagcga gttgagcggc gcggatatca tcatcgcttc tacgcacatt gcgggcgaaa
     1741 tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa catgctctct cctgccgact
     1801 ttggcccgaa actgctggaa gtgatcaaag cgcatttccc gcaggatgtg aagtaaggac
     1861 gcgccatgaa attacgtgat tcgctggcgg agaataaatc catccgcctg caggctgaag
     1921 ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct gctggtggcg gcggatgttg
     1981 tcgagccgcg ttactaccag gcgattctgg atgcggttga acagcacggt ccgtactttg
     2041 tgctcgcacc aggcctggca atgccgcacg gtcgcccgga agagggcgtt aagaaaaccg
     2101 gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa ccacgaagat aacgacccgg
     2161 tggatatcct catcaccatg gcggcggtcg atgccaacac gcaccaggaa gtgggcatca
     2221 tgcagatcgt caacctgttt gaagatgaag aaaatttcga ccgtttacgt gcctgccgta
     2281 ccgagcagga agtactggat ctcattgacc gcaccaacgc ggcagcttaa gaaggaattg
     2341 aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc agactatgga tagcgcctac
     2401 gaaaccactc gcctgattgc cgaagaagtc gacattatcg aagtgggtac cattctgtgc
     2461 gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc tctacccgca caaaatcgtg
     2521 ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt ctcgtatgtg cttcgaagcc
     2581 aacgctgact gggtgaccgt gatttgttgt gcggatatca acaccgccaa aggtgcgctg
     2641 gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac tgaccggtta ctggacctgg
     2701 gaacaggcgc aacagtggcg cgacgcaggt attcagcagg tggtttatca ccgcagccgt
     2761 gacgcgcagg ccgctggcgt ggcgtggggt gaagccgata tcactgcgat caaacgtctt
     2821 tccgatatgg gcttcaaagt caccgtcacc ggtggcctgg cgctggaaga tctgccgctg
     2881 ttcaaaggca ttccaattca tgtgtttatc gccggtcgca gcatccgtga tgccgcttct
     2941 ccggttgaag ccgcgcgtca gttcaaacgt tccatcgccg aactgtgggg ctaaggagcg
     3001 gatatgttgt ccaaacaaat cccgcttggc atctatgaaa aagcgctccc cgccggggag
     3061 tgctggctgg aacgcctgcg actggcaaaa acgttgggct tcgattttgt cgagatgtcg
     3121 gtagatgaaa ctgacgagcg cctgtcgcgc ctcgactgga gccgcgagca gcgtctggcg
     3181 ctggtgaatg cgattgttga aaccggcgtg cgtgtgccgt ccatgtgcct ttctgcccat
     3241 cgtcgtttcc cgttgggcag cgaagatgac gcggtgcggg cgcaggggct ggagattatg
     3301 cgtaaagcca tccagttcgc ccaggacgtt ggcattcgcg tgatccagct ggcgggctat
     3361 gacgtttact atcaggaagc caataacgaa acgcgtcgtc gtttccgtga cggcctgaaa
     3421 gagagcgttg agatggcgag ccgcgcgcag gtgacgctgg cgatggagat catggattat
     3481 ccgttgatga actccatcag caaggcgctg ggttacgcgc actatctcaa caacccgtgg
     3541 ttccagctct acccggatat cggcaacctg tcggcgtggg acaacgatgt gcagatggag
     3601 ttgcaggccg gaatcgggca tatcgtcgcg gtgcatgtga aagacaccaa accgggcgtc
     3661 ttcaaaaacg tgccatttgg cgaaggtgtg gtggatttcg aacgttgctt cgaaacgctc
     3721 aaacagagtg gctattgcgg gccgtacctg attgagatgt ggagcgaaac ggcggaagac
     3781 ccggcggcag aagtggtgaa agcgcgtgat tgggtgaaag cgcgcatggc caaagcaggc
     3841 acggtggagg cggcataatg caaaagctaa aacagcaggt gtttgaagcc aacatggatc
     3901 tgccgcgcta tgggctggtg acctttacct ggggcaacgt cagcgctatc gaccgcgaac
     3961 gcgggctggt ggtgatcaag cccagcggcg ttgcctacga aaccatgaaa gcagacgata
     4021 tggtggtggt cgatatgagc ggcaaggtgg tggaagggga gtatcgcccg tcttccgaca
     4081 ccgcgacgta tctcgaactc taccgtcgtt acccgtcgct tggcggcatt gtccataccc
     4141 actccactca tgctactgca tgggcgcagg cggggctggc gatcccggcg ttaggcacta
     4201 cgcacgccga ctacttcttt ggcgacatcc cgtgtacgcg cggattaagc gaagaagagg
     4261 tgcagggcga gtatgaactg aacaccggca aagtgattat cgaaacgctg ggtgacgccg
     4321 agccgctgca tacgccgggg attgtggtgt atcagcacgg tccgttcgcc tgggggaaag
     4381 atgcccacga cgcggtgcat aacgcggtgg tgatggaaga agtggcgaaa atggcgtgga
     4441 ttgcgcgcag tattaaccca caactgaatc acatcgacag cttcctcatg aataaacact
     4501 tcatgcgtaa gcacggtcct aacgcctatt acgggcagaa gtaagcagtg cgccccggaa
     4561 ttattacctc ctgcgggatg taattggtct gattccgggg ccgcttcagt taaaaaatcg
     4621 tataaacaac aggtgttttt atggctatca atggattgtt tgtcagaacg gatccgcaac
     4681 gtcgccgcta tggcgttgcg ttacttgtgg gtgtactttc aggaattatt tcagcatttg
     4741 ttaaatgggg agcggaagtc cccttgccac cgcgcacatt ttccggtggt cgcgatgaat
     4801 ttaacccgcc gtatatcttt ctacgggatt atttaggtat cgacccaacc caaactgtgt
     4861 atacattctc ggaacatatt atcaacccgg tgatggtgac gcatatcatc ttctcactgg
     4921 tttttgcgat agggtattgt attgtcgcgg aaattttccc gaaagtgaaa ttatggcagg
     4981 ggatattagc agggcttatt gtgacagtgg cagtccacgg tatattttgc ccggcgctga
     5041 atttaacgcc gccgctcact caattgcctt ttgacgaata tgcctctgaa atcctggggc
     5101 atattttctg gttctggata atagaaatta tgcgccgcga cttacgtaat cgcattactc
     5161 atgagccaga tccggaagtc gcaatccggt gaataaccgc ataaaaaagc cccctgcgaa
     5221 gggggcaaag caaactatgg caatgtttcg ttggttatac ctggtgctag cgataaatat
     5281 ccgcgctggc gtgcatattg ccgttactcc ccggttcccg catcagaatt acgtggtagt
     5341 acgttactcc ccggttcccg catcagaatt acgtggtagt acgttgcgcc ctgcgcatct
     5401 gtctcttcat ttaatgcctg acgcgcttcg ctttcagtgg cgaaattatg attgatataa
     5461 atcacgccta agctttgcac atcatccatg tttctggcct ggtggttatt aatttcaatg
     5521 gctgcccatg tatttgcact tagcaaaagc acagccagaa gggctaaaac tcgactgaac
     5581 atagatacct cctcgacggc tgactttgtg tgctctcctc tgtgatgatc ttctgattta
     5641 attttaatca atgataaaga agttgatggt gaccatttct gatgcagttg ttcaaaaaaa
     5701 caccatgatg aagtgtgatg aactccaaat cagcggttta gaggttaatt gcgaaagggg
     5761 agatttattt cggctctgcc cttgagttta gcggggcata caagtactat aacgcgtcat
     5821 ttttcagccg acctttaaca cgttccttgc ctccccggga ttcggctgac ccagacagga
     5881 ggctgaataa tccgtaagga gcaattcgat gcgtcattac gaaatcgttt ttatggtcca
     5941 tcctgaccag agcgaacagg ttccgggcat gatcgagcgc tacactgctg ccatcactgg
     6001 tgcagaaggc aagatccacc gtctggaaga ctggggccgc cgtcagctgg cttacccgat
     6061 caacaaactg cacaaagcac actacgttct gatgaacgtt gaagctccgc aggaagtgat
     6121 cgatgagctg gaaactacct tccgcttcaa cgatgccgtt atccgcagca tggttatgcg
     6181 taccaagcac gctgttaccg aagcatctcc gatggttaaa gcgaaagacg agcgccgtga
     6241 gcgtcgcgat gatttcgcaa acgaaaccgc tgatgatgct gaagctgggg attctgaaga
     6301 gtaatttctg atgaccaacc gtctggtgtt gtccggcacc gtgtgcagga ctccccttcg
     6361 aaaggtcagt ccatcaggaa ttcctcactg ccagttcgtg cttgagcatc gttctgtgca
     6421 ggaggaagcc ggttttcacc ggcaggcgtg gtgccaaatg cccgttattg ttagcggaca
     6481 cgaaaaccag gccattactc acagtataac ggtcggcagt cgcataaccg ttcaggggtt
     6541 catttcatgc cacaaggcaa agaacggact gagcaaaatg gttttgcatg ccgagcagat
     6601 tgaattgata gattctggag actagccata tggcacgtta tttccgtcgt cgcaagttct
     6661 gccgtttcac cgcggaaggc gttcaagaga tcgactataa agatatcgct acgctgaaaa
     6721 actacatcac cgaaagcggt aagattgtcc caagccgtat caccggtacc cgtgcaaaat
     6781 accagcgtca gctggctcgc gctatcaaac gcgctcgcta cctgtccctg ctgccgtaca
     6841 ctgatcgcca tcagtaatcg gtcacggtcc attaatacga ctttgagagg ataaggtaat
     6901 gcaagttatt ctgcttgata aagtagcaaa cctgggtagc ctgggtgatc aggtaaacgt
     6961 taaagcgggc tatgctcgta acttcctggt accgcagggt aaagctgttc cagctaccaa
     7021 gaaaaacatt gaattcttcg aagcacgtcg cgctgaactg gaagctaaac tggctgaagt
     7081 tctggcagct gctaatgctc gcgctgagaa aatcaatgca ctggaaactg ttaccatcgc
     7141 gtctaaagct ggcgacgaag gtaaactgtt cggttccatc ggtactcgcg acatcgctga
     7201 cgctgtaact gcagctggcg ttgaagtggc taagagcgaa gttcgtctgc cgaacggcgt
     7261 tctgcgtacc actggcgagc acgaagtgag cttccaggtt cacagcgaag tattcgcgaa
     7321 agtgatcgta aacgtagtag ctgaataatt cgttattcaa cgagacgtaa aaacgccgac
     7381 cagtggtcgg cgttttgctt ttgtagcgag cacttgcaga tatcgataag ggccacaata
     7441 agcagcccta cgaagataat tacttagctg cctgaatatc agccagcatt ggggcaaggg
     7501 ctgggttgtc cttagcgaag tttgcgtaca ttggctgtac tttctcgata aacggagctt
     7561 tatctacctg gtagactttc acgcccattt tatccagttt tgcaaattct tgtttctctt
     7621 cctcgctcca caagtctttc atgtagctca ttgattcttt agctgctttt aatataactg
     7681 cctgattttc cgggctaagt ttatcaaacg cagcattgct catcagaacc acatcgggaa
     7741 ccatagtgtg ttcgtcaagg ctgaaggatt ttgccacttc accgtgacgc atggtggtca
     7801 gcgccatcac gctgttttct gccatatcga ccacaccctg ctgtaaggct gtatacaact
     7861 cgccgtaatc cagtggcgtc gggttaccgc ccatgacttt gatcatttca actgcgccag
     7921 ggcttggctg gacacggact ttcatgcctt tcagatcgtc tggtttcagt actggtttat
     7981 tgccatagaa actgcgggcg cctccatcat aaaaagtcag cccgaaataa cctttgcttt
     8041 tcgttgagtc gaggattttt ctcccgatat cgccctgcat gacctgataa taatgatcac
     8101 gatcgcggaa caaataaggc aggctaaata atttatagga attttcaaat gcttccattt
     8161 ctgccgcatt ggtttttacc agtggaatag cgccagaacg aatcagctcc attgtttctc
     8221 gttgagtacc caatgttcca tttggataaa tacgaatagt aatatcaccg ttagagtact
     8281 ctttgttctt tttcgcaaag aactccatcg ctttatgaac aggatgagac ttatcctgat
     8341 tatgactcag ttttaatgtc actgcccagg actggcttgc ggataacata agaacagcac
     8401 cgataatgcc agcggatact tttattttca tagatgttcc ttttctaata aatatggcgg
     8461 caggtgccgc cagtgaatgc gcatcagtag cccagtgctt ttggcaatgc catactgatt
     8521 tgcggaatat aggtcaccat tgccagcacc aataccatca cggcatagaa tggcagcata
     8581 ggtttgataa ttttattaat cggtaattta gatacactac aaccgacaaa taacacactg
     8641 cctacaggcg gagtacaaat accgatacac atgttcagta ccatgacaat accaaagtga
     8701 atcgggtcca tgcccagttc ggtgacaaca ggtaaaaata tcggcgtaaa aatcagtacc
     8761 gccggtgtca tatccataaa agtgccgata atcaacagga taatattgat aataagcagg
     8821 attattattg ggttgtcaga aatagtgtta agcaaatcga gaatcaggaa gggaacgtca
     8881 gcgtttgaca ttgcccatga cggtaatgac tccaacttat tgatagtgtt ttatgttcag
     8941 ataatacccg atgactttgt catgcagctc caccgatttt gagaacgaca gcgacttcc
//
LOCUS       DAZOXR010000169         8839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZOXR010000169 DAZOXR010000000
VERSION     DAZOXR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.175"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUNN_003485"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUNN_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0577767.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUNN_003486"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUNN_003486"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR0577768.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHUNN_003487"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHUNN_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUNN_003488"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUNN_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0577769.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUNN_003489"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUNN_003489"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0577770.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHUNN_003490"
     CDS             complement(6490..6846)
                     /locus_tag="ACHUNN_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0577771.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHUNN_003491"
     CDS             complement(6850..7266)
                     /locus_tag="ACHUNN_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0577772.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUNN_003492"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUNN_003492"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0577773.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="ACHUNN_003493"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="ACHUNN_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2256 a         2177 c         2149 g         2257 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataag
//
LOCUS       DAZOXR010000170         8638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZOXR010000170 DAZOXR010000000
VERSION     DAZOXR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.176"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(260..523)
                     /gene="rpsT"
                     /locus_tag="ACHUNN_003494"
     CDS             complement(260..523)
                     /gene="rpsT"
                     /locus_tag="ACHUNN_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0577774.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            626..844
                     /locus_tag="ACHUNN_003495"
     CDS             626..844
                     /locus_tag="ACHUNN_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0577775.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            852..1793
                     /gene="ribF"
                     /locus_tag="ACHUNN_003496"
     CDS             852..1793
                     /gene="ribF"
                     /locus_tag="ACHUNN_003496"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0577776.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1836..4652
                     /gene="ileS"
                     /locus_tag="ACHUNN_003497"
     CDS             1836..4652
                     /gene="ileS"
                     /locus_tag="ACHUNN_003497"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0577777.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4652..5146
                     /gene="lspA"
                     /locus_tag="ACHUNN_003498"
     CDS             4652..5146
                     /gene="lspA"
                     /locus_tag="ACHUNN_003498"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0577778.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5271..5720
                     /gene="fkpB"
                     /locus_tag="ACHUNN_003499"
     CDS             5271..5720
                     /gene="fkpB"
                     /locus_tag="ACHUNN_003499"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0577779.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5722..6672
                     /gene="ispH"
                     /locus_tag="ACHUNN_003500"
                     /gene_synonym="lytB"
     CDS             5722..6672
                     /gene="ispH"
                     /locus_tag="ACHUNN_003500"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0577780.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6738..7652
                     /gene="rihC"
                     /locus_tag="ACHUNN_003501"
     CDS             6738..7652
                     /gene="rihC"
                     /locus_tag="ACHUNN_003501"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0577781.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7819..>8638
                     /gene="dapB"
                     /locus_tag="ACHUNN_003502"
     CDS             7819..>8638
                     /gene="dapB"
                     /locus_tag="ACHUNN_003502"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0577782.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1966 a         2158 c         2458 g         2056 t
ORIGIN      
        1 atgtgtatca caatatccac attctttgta ggggagtctg gagattgagt agatattctt
       61 gttcagaatg tatcagccga tggttctacg attcttaagc cacgaagagt tcagaaagtg
      121 taacggcatg tctgcaaaag ccagcatctt ttgactatct ggcaaccggt agtgtgttct
      181 ctcacgcatc ataaaagcag caggcataaa aaaaacccgc ttgcgcgggc tttttcacaa
      241 agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat
      301 gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt
      361 gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata
      421 ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt
      481 cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg
      541 tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat
      601 ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg
      661 attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg
      721 tcactttctt cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt
      781 actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt
      841 ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag
      901 ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac
      961 agggcttgca gaaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac
     1021 ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc cggctgcggg
     1081 aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca
     1141 ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc
     1201 gcgtaaaatt tcttgccgta ggggatgatt tccgctttgg cgctggtcgt gaaggcgatt
     1261 tcttgttatt acagaaagct agcatggaat acggcttcga tatcactagt acgcaaactt
     1321 tttgcgaagg tggcgtgcgt attagcagca ccgctgtgcg tcaggcgctg gcggatgaca
     1381 atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc
     1441 acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc
     1501 aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt
     1561 tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcagctgg
     1621 aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc
     1681 gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg
     1741 cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt
     1801 atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga
     1861 atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc
     1921 tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa
     1981 aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact
     2041 cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact
     2101 cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag
     2161 aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat
     2221 acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg
     2281 actggtcgca cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc
     2341 tgggcaaaat catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg
     2401 ttgactgccg ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt
     2461 ccatcgacgt tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa
     2521 gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg
     2581 cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc
     2641 aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg
     2701 attacaccat tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc
     2761 cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttacccta gatgccggta
     2821 ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat
     2881 acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc
     2941 cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg
     3001 aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc
     3061 acaaaacgcc gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag
     3121 gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc
     3181 aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca
     3241 cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta
     3301 ctctcgaact aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt
     3361 gggatcttga tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg
     3421 acacattgga tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc
     3481 cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt
     3541 ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg
     3601 tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca
     3661 ataccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg
     3721 tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgctg
     3781 ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt
     3841 ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg
     3901 taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc
     3961 acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg
     4021 acatcatcaa agaccgtcag tacaccgcca aagcggacag tgtggcgcgt cgtagctgcc
     4081 agactgcgct gtatcacatc gcagaagcgc tggtgcgctg gatggcacca atcctctcct
     4141 tcaccgctga tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac gtcttcaccg
     4201 gtgagtggta cgaaggcctg tttggcctgg cagacagtga agcgatgaac gatgcgttct
     4261 gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg
     4321 acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgt
     4381 cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta
     4441 ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc
     4501 tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc
     4561 aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg
     4621 ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct
     4681 acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat
     4741 cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta
     4801 tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg
     4861 gttctttgcc ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc
     4921 gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg gcgcgctggg
     4981 caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg
     5041 cgactggcac ttcgccacct tcaaccttgc cgatactgcc atctgtgtcg gtgcggcact
     5101 gattgtgctg gaaggttttt tgccttctag agcgaaaaaa caataataaa ccctgccgga
     5161 tgcgatgctg acgcatctta tccggcctac agattgctgc gaaatcgtag gccggataag
     5221 gcgtttacgc cgcatccggc aaaaatcctc aaatataaga gcaaacctgc atgtctgaat
     5281 ctgtacagag caatagcgcc gtcctggtgc acttcacgct aaaactcgac gatggcacca
     5341 ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt gatgcttctc
     5401 tttctgaagg gctggagcaa cacctgttgg ggctgaaagt gggcgataaa accaccttct
     5461 cgttggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttttccc
     5521 gccgtgaatt tatggatgca ggcgagccag aaattggcgc aatcatgctt tttaccgcaa
     5581 tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgttg
     5641 atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg
     5701 atccggcatt ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg tttttgtgcc
     5761 ggggtagacc gcgctatcag cattgttgaa aacgcgctgg ccatttacgg cgcaccgata
     5821 tatgtccgtc acgaagtggt acataaccgc tatgtggtcg atagcttgcg tgagcgtggg
     5881 gctatcttta ttgagcagat tagcgaagta ccggacggcg cgatcctgat tttctccgca
     5941 cacggtgttt ctcaggcggt acgtaacgaa gcaaaaagcc gcgatttgac ggtgtttgat
     6001 gccacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag ccgtcgtggc
     6061 gaagagtcga ttctcatcgg ccacgccggg catccggaag tggaaggtac aatgggccag
     6121 tacagtaacc cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt gtggaaactg
     6181 acggtcaaaa acgaagagaa gctctccttt atgacccaga ccacgctgtc ggtggatgac
     6241 acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa
     6301 gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag
     6361 gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag
     6421 ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga tatccaggaa
     6481 gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt
     6541 ctggtgcaaa atgtggtggc acgtttgcag cagctgggcg gtggtgaagc cattccgctg
     6601 gaaggccgtg aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt
     6661 gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttaccggca
     6721 ttttttatgg agaaaacatg cgtttaccta tcttcctcga tactgacccc ggcattgacg
     6781 atgccgtcgc cattgccgcc gcgatttttg cccccgaact cgacctgcaa ctgatgacca
     6841 ccgtcgcggg taatgtctcg gttgagaaaa ccacccgcaa cgccctgcaa ctgctgcatt
     6901 tctggaatgc ggagattccg ctcgcccagg gagccgctgt gccgctggta cgcgcaccgc
     6961 gtgatgcagc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca
     7021 accgaaagcc aatcgggata ccagcgtttc tggcgattcg tgatgccctg atgcgtgcac
     7081 cagagcctgt taccctggtg gccattggcc cgttaaccaa tattgcgctg ttactttcac
     7141 aatgcccgga atgtaaaccg tatattcgtc gtctggtgat catgggcggt tcttctggtc
     7201 gcggcaactg tacaccaaat gccgagttta atatcgctgc cgatccagaa gcggctgcct
     7261 gtgtcttccg tagcggaatc gaaatcgtca tgtgcggtct ggatgtcacc aatcaggcaa
     7321 tattaacccc tgactatctc gctactttgc cggagttgaa tcgcaccgga aaaatgcttc
     7381 atgcactgtt tagccactac cgtagcggca gtatgcaaag cggtctgcga atgcacgatc
     7441 tctgcgccat cgcctggctg gtgcgcccgg acttattcac cctcaagccg tgttttgtcg
     7501 cggtagaaac tcaaggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc
     7561 tgggcaaacc agccaatgta aaggtggcat tggatctgga tgtgaaaggc ttccagcagt
     7621 gggtggctga ggtgctggct ctggcgtcgt aacctgtcac atgttatcgg catgcagtca
     7681 ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc
     7741 agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat
     7801 taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg
     7861 gggcgtatgg gccgccagtt gattcaggcg gcgctggcat tagaaggcgt gcagctgggc
     7921 gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt
     7981 gcagggaaaa caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga tgattttgat
     8041 gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag
     8101 catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt
     8161 cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac
     8221 gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatatcgaa
     8281 attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg
     8341 ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt
     8401 gaaggccaca ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt
     8461 gacatcgttg gtgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc
     8521 cataaggcgt ccagccgtat gacatttgct aacggcgcgg taagatcggc tttgtggttg
     8581 agtggtaagg aaaatggtct ttttgatatg cgagatgtac ttaatctcaa taatttgt
//
LOCUS       DAZOXR010000171         8634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZOXR010000171 DAZOXR010000000
VERSION     DAZOXR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.177"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUNN_003503"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUNN_003503"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0577783.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUNN_003504"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUNN_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0577784.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHUNN_003505"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHUNN_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0577785.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHUNN_003506"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHUNN_003506"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0577786.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHUNN_003507"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHUNN_003507"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0577787.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHUNN_003508"
     CDS             complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHUNN_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0577788.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHUNN_003509"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHUNN_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0577789.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2179 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaa
//
LOCUS       DAZOXR010000172         8575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZOXR010000172 DAZOXR010000000
VERSION     DAZOXR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.178"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..154
                     /locus_tag="ACHUNN_003510"
     CDS             <1..154
                     /locus_tag="ACHUNN_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR0577790.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="ACHUNN_003511"
     CDS             258..1172
                     /locus_tag="ACHUNN_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0577791.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="ACHUNN_003512"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="ACHUNN_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0577792.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="ACHUNN_003513"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="ACHUNN_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0577793.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="ACHUNN_003514"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="ACHUNN_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0577794.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="ACHUNN_003515"
     CDS             5467..7149
                     /locus_tag="ACHUNN_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0577795.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="ACHUNN_003516"
     CDS             7201..8358
                     /locus_tag="ACHUNN_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0577796.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="ACHUNN_003517"
     CDS             complement(8324..8446)
                     /locus_tag="ACHUNN_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577797.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT         2521 a         1829 c         1836 g         2389 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cgatggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cataaccact gaaaattaaa aatttctata tattt
//
LOCUS       DAZOXR010000173         8534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZOXR010000173 DAZOXR010000000
VERSION     DAZOXR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.179"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..359)
                     /gene="mhpC"
                     /locus_tag="ACHUNN_003518"
     CDS             complement(<1..359)
                     /gene="mhpC"
                     /locus_tag="ACHUNN_003518"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0577798.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSS"
     gene            complement(377..1321)
                     /gene="mhpB"
                     /locus_tag="ACHUNN_003519"
     CDS             complement(377..1321)
                     /gene="mhpB"
                     /locus_tag="ACHUNN_003519"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR0577799.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1323..2987)
                     /locus_tag="ACHUNN_003520"
     CDS             complement(1323..2987)
                     /locus_tag="ACHUNN_003520"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR0577800.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3029..3862
                     /locus_tag="ACHUNN_003521"
                     /pseudo
     CDS             3029..3862
                     /locus_tag="ACHUNN_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            3939..5021
                     /gene="lacI"
                     /locus_tag="ACHUNN_003522"
     CDS             3939..5021
                     /gene="lacI"
                     /locus_tag="ACHUNN_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR0577801.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5144..8218
                     /gene="lacZ"
                     /locus_tag="ACHUNN_003523"
     CDS             5144..8218
                     /gene="lacZ"
                     /locus_tag="ACHUNN_003523"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR0577802.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8270..>8534
                     /locus_tag="ACHUNN_003524"
     CDS             8270..>8534
                     /locus_tag="ACHUNN_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0577803.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2390 c         2335 g         1868 t
ORIGIN      
        1 gaactatggc cgcccatcga gttgcccagc aggtggattt tggcgatatc cagttgatcc
       61 accacgcttt tcaggattcg tgcattaaga tccgatcgtg aaccactatt aacgatcgaa
      121 tcgcttttgc cccaacccgg acaatccagc aggatcaccc gatagcccgc ctctaccagc
      181 ggatcgatat tgcggctgaa gttcgcccag ccagtagcac ccgggccgga accatgcagc
      241 aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat ggatgcgcag cgttttaccc
      301 gcttcttcta cattcagaaa acggctggtg gcggcttcgg tttgtggctg ataactcatc
      361 atcttctcct gcatattcag ttctctgttc tggcgcttaa cgagccaaat ccggcaatcc
      421 actccgggat tgggcggtaa taacgccctt cgctacgcca gttgccaaat gcagaaatag
      481 cggcaaaagc ggcgacccag gttttgattt catgtgtcga ctttccggca atggcggaaa
      541 gctcttcgtt actgacggca tccagttcct gtatgcgtcc ctgctccagc aaagtcatga
      601 actggttatc ccaaatcggg ttgagcggat gcagagttct ctgatcctca acaaacttct
      661 cagcggcgct aatcaccctt tgctgacgca attcgcgctc actggcgggt aaatctttcc
      721 cgctccccaa cagacggtcg cgcatatggg catcggcttt cgccagttcg ggcaccggcg
      781 gctgatggga aagcccaccg gaacccagga acagcacgcg tttattgaga gtgctggtga
      841 aacgtccaat ggcttcaccc aacatgcggg tacgctggaa accgggcagc ggcgtggcga
      901 caccgttgat gaacacaggc agaactggca ccttatccag cccaccgagc aggaactcca
      961 gcggctgggc gaacccgtgg tccacctgca tacagtaaga aacggcaaga tcgatcccgc
     1021 tcttcatgac agcatgcgca caggcctccg ccagctccac aggcacgggc agctctcctg
     1081 ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc taaacagaac ggtggcatca
     1141 cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag caccaccagt tcaggggaga
     1201 aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt gacctcatcg agtacctctt
     1261 gcgccgggtc gacatacccc accaacggcg agtgggaaag acagtgaaga taagcgtgca
     1321 tatcaggcta ccttttcgac agaaacgtcg gcatcagggc gggtcagcgt catcaccgac
     1381 gccagtttat tcagcgtatt gcccagggtt tgcggaatgg cggtggcggc aacaaagcga
     1441 tccgggcgca tcaccaccag cgaagcattg tgttgcgcga accagctacg cagacgacct
     1501 tgcgtatcgc ccacgcgtag cacgccgtcg tggttatcct gtgcggtatg aatttgcact
     1561 tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc actgctggat ttgctcgtcg
     1621 ctcatccccc acagtggatt gcatccccag ccaattaccg cgaagttcgc gccgatcgcg
     1681 ttatcgagca gcgtcacgtc gccgttttcc agcgtgactt tcggctgaat aaacatcttg
     1741 ccgaccggag agtgcttcgc ttcgccctca cgcaccagcg caccgccgta atattgcggc
     1801 atcggcttga agcgcatttc gaggaagtag cgttttactg gcggcagata attcaacagc
     1861 caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg gcggagccag cacgttgccc
     1921 gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc gacgttcttg ttgataggta
     1981 tcgagcagcg catcgcgggc tttcccctgg ataaccaacg ccagtttcca ggcgaggtta
     2041 aaggcgtcgc gcataccact gttatagccc tgcccctgcc acaccggcat gatgtgcgcg
     2101 gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt gcgccagtcg cgcgttatgg
     2161 gtgtagacac gctggcgaat caattcaaca ttgtccggat taggcagcac tttgcttaac
     2221 agcttgcgca tattttgcgg ctcacgcagc tgctcttcgg tttctcccgg catcaccata
     2281 aattcaaagc gacgtaccgc atgaggcagc gcggcagaaa catacgggcg caccggatcg
     2341 caacacaaat agatatgcgg cgtacttaac ggatcgttgg cgatatctac cacaatccac
     2401 tgatttggcg cagttttacc ttcaaacggc acattcaggg tgcgacgaac aaaacttgcc
     2461 ccaccatcac aggccaccag ccactgggct ttgatcgttt cccgctgccc ttctgccgtt
     2521 ttcaggtgca aggtcacttc gtcatcttgc tggctgaagg cctccagctc gcgggaaaac
     2581 aagcagcgca cattcggaaa acgcgacaac ccttccagca tcaccgcatc gacctgtggc
     2641 tgaataaagg cgttacggcg cggccagcca aattcatcgg tcattggctg aatatcagca
     2701 aaacagcggc cttttggggt gagaaaacgc atcgcgtgcc acggtgtagt gtgcggcaga
     2761 acattctcga ccaggccgac cgactgcatg gtgcgcagcg cctcgtcatc aataccaatc
     2821 gcacgcgggt agtcgatcaa cttatcgagt ttctccacca ccagcacgtc aatgcccatc
     2881 tgaccgagat agttcgccat catcagccca accggaccgg caccagcgat cgccacctga
     2941 acgctatggt taacagcagg ctggatgtca gggtgttgta ttgccatttc tgcacaacgt
     3001 tgttttaact atagaaatgt caattaatat gcagaacaat gagcagacgg aatacaaaac
     3061 cgtgcgcggc ttaacccgcg gtctaatgtt attaaatatg ttaaataaac ttgatggcgg
     3121 tgccagcgtc gggctgctgg cggaactcag cggcctgcat cgcaccactg tgcggcgact
     3181 gctggagacg ctgcaggaag agggatatgt ccgccgtagc ccctccgatg acagttttcg
     3241 tctgaccatc aaagtgcggc aattaagcga aggatttcgt gacgaacagt ggatttctgc
     3301 actggcggcc ccgctgctgg gcgatctgtt gcgcgaagtg gtatgaccaa cagatgtgtc
     3361 caccctggat gttgatgcaa tggtggtacg cgaaaccacc caccgtttca gccgcttatc
     3421 ctttcaccgg gcaatggtcg ggcgacgttt accgctcctg aaaaccgcct cgggcctgac
     3481 ctggctggcc ttttgcccgg aacaagaacg caaggaatta atcgaaatgt tagccgcccg
     3541 ccccggtgat gactatcaac tggcacgaga accgttaaag ctgcaagcca ttctggcgcg
     3601 cgcgcgcaaa gagggttacg gacagaacta ccgcggctgg gatcaggagg agaagatcgc
     3661 ctctatcgcc gtaccgctgc gcagtgaaca acgggtgatt ggctgtctga atctggtgta
     3721 tatggcgagc gcaatgacca ttgaacaggc agcggaaaag catcttccgg cgctacaacg
     3781 ggtagcaaaa cagatcgaag aaggggttga atcgcaggct attctggtgg ccggacggcg
     3841 aagcggcgtg catttacgtt gacaccatcg aatggcgcaa aacctttcgt ggtatggcat
     3901 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgctatacg
     3961 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc tcgcgtggtg aaccaggcca
     4021 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     4081 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     4141 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     4201 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     4261 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     4321 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     4381 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     4441 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     4501 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     4561 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     4621 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     4681 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     4741 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     4801 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     4861 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     4921 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4981 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5041 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5101 tgtgaaattg tgagcggata acaatttcac acaggaaaca gctatgatca tgattacgga
     5161 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5221 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5281 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
     5341 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
     5401 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
     5461 tcccattacg gtcaatccgc cttttgttcc cgcggagaat ccgacgggtt gttactcgct
     5521 cacatttaat attgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
     5581 tgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
     5641 ccgtttgccg tctgaatttg acctgagcgc atttttacgc gccggaaaaa accgcctcgc
     5701 ggtgatggtg ctgcgctgga gtgacggtag ttatctggaa gatcaggata tgtggcgaat
     5761 gagcggcatt ttccgtgacg tctcgttgct gcacaaaccg agcacacaaa tcagcgattt
     5821 ccatgttgcc actcacttta atgatgattt cagccgcgcg gtactggagg cagaagttca
     5881 gatgtacggc gagctgcgcg atgagctgcg ggtgacggtt tctttgtggc agggtgaaac
     5941 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
     6001 tgccgatcgc gtcacgctac gtctgaacgt cgaaaacccg gcgctgtgga gcgccgaaat
     6061 cccgaatctc tatcgtgcgg tggttgaact gcacaccgac gacggcacgc tgattgaagc
     6121 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
     6181 cggcaagccg ttgctgattc gcggcgttaa ccgtcacgag catcatcctc tgcatggtca
     6241 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
     6301 cgccgtgcgt tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgctt
     6361 cggcttgtac gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
     6421 tctgaccgat gatccgcact ggttacccgc gatgagcgaa cgcgtaacgc gaatggtgca
     6481 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgagt caggccacgg
     6541 cgctaatcac gacgcactct atcgctggat caaatctgtc gatccatccc gcccggtgca
     6601 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
     6661 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
     6721 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
     6781 tcttggcggc ttcgctaaat actggcaggc gtttcgtcag tacccccgtt tacagggcgg
     6841 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaatg gcaatccgtg
     6901 gtcggcttac ggcggtgatt ttggcgatac accgaacgat cgccagttct gcatgaacgg
     6961 tctggtcttt gccgaccgca cgccgcatcc agcgctgaca gaagcaaaac accagcagca
     7021 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
     7081 tcatagcgat aacgagcttc tgcactggtc ggtggcgttg gatggcaagc cgctggcaag
     7141 cggtgaaatg cctctggatg ttgctccaca agataaacag ttgattgaac tgcctgaact
     7201 accgcagccg gagagcgccg gacaactctg gcttacggta cacgtagtgc aaccgaacgc
     7261 gaccgcatgg tcagaagccg gacacatcag cgcctggcag cagtggcgtc tggcggaaaa
     7321 cctcagcgtg acactccccg ccgcgcccca cgccatcccg caactgacca ccagcgaaac
     7381 ggatttttgc atcgagctgg gtaataaacg ttggcaattt aaccgccagt caggctttct
     7441 ttcacagatg tggattggcg ctgaaaaaca actgctgacg ccgctgcgcg atcagttcac
     7501 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg acccgaacgc
     7561 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
     7621 cacggcagat acacttgccg acgcggtgct gattacgacc gctcacgcgt ggcagcatca
     7681 gggaaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
     7741 gattaccgtt gatgttgaag tagcgagcga tacaccgcat ccggctcgga ttggcctgac
     7801 ctgccagctg gcgcaggtag cagaacgggt aaactggctc ggattagggc cgcaagaaaa
     7861 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
     7921 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
     7981 ttatggccca caccagtggc gcggcgactt ccagttcaat atcagtcgct acagccaaca
     8041 acaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
     8101 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtttcagc
     8161 ggaattccag cttagcgccg gtagctacca ttaccagttg gtctggtgtc aaaaataata
     8221 ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt
     8281 aaaaaacaca aacttttgga tgttcggttt attctttttc ttttactttt ttatcatggg
     8341 ggcctacttc ccgtttttcc cgatttggct acatgacatc aaccatatca gcaaaagtga
     8401 tacgggtatt atttttgccg ctatttctct gttctcgcta ttattccaac cgctgtttgg
     8461 tctgctttct gacaaactcg ggctgcgcaa atacctgctg tggattatta ccggcatgtt
     8521 agtgatgttt gcgc
//
LOCUS       DAZOXR010000174         8486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZOXR010000174 DAZOXR010000000
VERSION     DAZOXR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.180"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..2519)
                     /gene="traC"
                     /locus_tag="ACHUNN_003525"
     CDS             complement(<1..2519)
                     /gene="traC"
                     /locus_tag="ACHUNN_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR0577804.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG"
     gene            complement(2645..2992)
                     /locus_tag="ACHUNN_003526"
     CDS             complement(2645..2992)
                     /locus_tag="ACHUNN_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577805.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(3020..3238)
                     /locus_tag="ACHUNN_003527"
     CDS             complement(3020..3238)
                     /locus_tag="ACHUNN_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577806.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(3337..3810)
                     /locus_tag="ACHUNN_003528"
     CDS             complement(3337..3810)
                     /locus_tag="ACHUNN_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577807.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(3803..4216)
                     /locus_tag="ACHUNN_003529"
     CDS             complement(3803..4216)
                     /locus_tag="ACHUNN_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577808.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(4209..4430)
                     /gene="traR"
                     /locus_tag="ACHUNN_003530"
     CDS             complement(4209..4430)
                     /gene="traR"
                     /locus_tag="ACHUNN_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR0577809.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(4565..5080)
                     /gene="traV"
                     /locus_tag="ACHUNN_003531"
     CDS             complement(4565..5080)
                     /gene="traV"
                     /locus_tag="ACHUNN_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR0577810.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(5077..5397)
                     /locus_tag="ACHUNN_003532"
     CDS             complement(5077..5397)
                     /locus_tag="ACHUNN_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR0577811.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(5384..5974)
                     /gene="traP"
                     /locus_tag="ACHUNN_003533"
     CDS             complement(5384..5974)
                     /gene="traP"
                     /locus_tag="ACHUNN_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR0577812.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(5964..7391)
                     /gene="traB"
                     /locus_tag="ACHUNN_003534"
     CDS             complement(5964..7391)
                     /gene="traB"
                     /locus_tag="ACHUNN_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR0577813.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(7391..8119)
                     /gene="traK"
                     /locus_tag="ACHUNN_003535"
     CDS             complement(7391..8119)
                     /gene="traK"
                     /locus_tag="ACHUNN_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR0577814.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(8106..>8486)
                     /gene="traE"
                     /locus_tag="ACHUNN_003536"
     CDS             complement(8106..>8486)
                     /gene="traE"
                     /locus_tag="ACHUNN_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR0577815.1"
                     /translation="NSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRPGAQN
                     QMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHY
                     ILILKRENGVTWLDNFGETDDEKK"
BASE COUNT         1985 a         2324 c         2027 g         2150 t
ORIGIN      
        1 ccctccctgc gtttctgctg cacaaactca aaatccgggc cgtcagagct gtacatggcg
       61 cggcttaacg ggtccacaaa cagacggtgc caggaggaat ggttttccac ctgcagcagg
      121 aaggaactga accactggtc tttggccgca ccaaacttgc caatcatgtc gcgctgcagt
      181 ggctggaact gatccgggaa taactggtta tatttcgcga actccttcgc actctgtttg
      241 aggataattt tgtaggagga gttaccccat gccgcacggg ccgcactgga ggccttgtca
      301 gagtcaaagt cgacgatgtt ctgtgtgatg gtgatatagg caccggtatg gcggcgacag
      361 gtgcggtaac ctttttgaat aaactcaccg accttgcgat ttttgaagtc cagcagacgc
      421 cagccttcat caatgacgtt cagtttcttg agagtacgcg gcgtgcggta catcctgttc
      481 tcgatgtaga taatcaggga gaacatcacc gcaaccagca gtgacggacg gtcttccagt
      541 ccgcccagct ccagcaccac cattttcgca tcatcccgca gggacggctc atcggagtta
      601 aaatactggc cgtaagtgcc gttggcagtg tactggtcaa gcagcacaat catttcgtcc
      661 agacggctgc ggatggtcgg tgacccggca tactgctcgc tgtcactggc gtttttcagg
      721 aaatccacca cgtcatcaat acgtgccctg ttctctttgg ccagccagga ggccctgacc
      781 gcctgcagca gcagaccttc atgcacttca tccaggttac cgttggggct ggccatcact
      841 gacaactggt cacggacacg ctccgctgac tggtcaatat cggtgatgtt cgcaaacggg
      901 ttaaaacgca gggtttcacc gtccagatat acgcccccca tgttctcaca cagggacttg
      961 tagccatccc ccatgtcgaa caccacggca aagcccccgg agtccagcac gctgcggata
     1021 agtggctgta tcagtccggt tttaccggcc ccggaggtgc cacagaccgc catgttgtag
     1081 ttggtgttat tcatcccctt aaagaagata tcgataaacg ccagctggtt acggtaggtg
     1141 ggcgccagca gacctgccgg tgtcaggggg ttatccgcca ctaacggcat caggttggcc
     1201 acattaaagc tctctgcgcg ctgaaccaca ccggcctctt tcagctgttt aaacagccct
     1261 ttcccggcca taaagggcag gcaggtcagg aaattgcgca tgtggttaaa gcgcggtgaa
     1321 atcagctcaa aaccgttttt acggaagctg ttcaggatgt cctgctccac ttccagtgcc
     1381 gtttcattat tgtccttgca gaaggctgtg atgttgagga agtaggacac gacagaagac
     1441 tggccggagc ccagccgctg gcgcagttcc ccccactcct tcgcctcttt ctccacggac
     1501 ggaaaccatt tggcatatga ggtcttcgac ttcttctcca ggtccatgta cttcaggttg
     1561 gcttcgctgt gggttttcac ctggtcttcc accaccagag tcagcgtcag gatgaacggg
     1621 caggaaatgg acatttccgg gttcagcaga ttgctgtagt tgtcggccat gttccacagg
     1681 aaggcgattt ccgggttacg ggccagatgg aaattcagga tgcgggccgt gctgttcctg
     1741 ccgttctcac gcaggcccag cgtcaggtaa tcagcccgga cattcaggtc aaaactgtcc
     1801 tccacacact gataattcag atcagaatac gggtccagct gacgtctttt cgggtacaga
     1861 gagtccggat tatggttaat catctccccg acaatctcga tgaaagcctg tgcatccact
     1921 gtctgtgtgg tgatttttgc gccgtgaaaa gaggcacgga tgattttcac cagattttcc
     1981 atttccagaa tgtcagcccg gctttttttc ttcgagggag aacagtacga gataaacacc
     2041 cggtaatgac gcagggtcag gggcagattc atcccctccg gcagcgggaa ctgtgtcgct
     2101 gccgctttca tataataggc acgggtaatg gcgttaaacc gttcagcctg ttcacctgac
     2161 caggagaact cacgcagccc gtattcaatc ctgtcaccaa ccagctgact ggacatcaga
     2221 tggatacaca acggaatacc gcgcggcagt ttggtgcgca gcatgtgatc gagtgcctcc
     2281 acaatggact tattcgcccc attgatggga atggcttcca gcataaagcc catcgtggtg
     2341 tcattcatga acagaccgga ttcctggttg taatcacggt acggcagcag acggctgaac
     2401 tgcgggaagc tcatttcgcc cagaacttca ttggcctttg cggattcgtc aggcagtttc
     2461 agtgctgtga cgagggagtt aaccgcctga gtgacggcct caagtgggtt attactcata
     2521 tgagtattcc ttagtgcgtt taaaaaagga aaatatcagt ctgtggccgt ggatatatca
     2581 cgcagtgcca ttcctgaaac cacagcacaa ataagagatg gaggctttta acaggaaacc
     2641 aggattaacg ttcctttact gaatgagcat catttaatgc ctgaatttgt ttttcgcgaa
     2701 tcttattgcg ctcattttct cttacccgtt tcacctctgc catttcgttt ctttttctgc
     2761 attctgatgc gattttattt gccttaccaa gtgttaaagg ttcagactga taattaagtg
     2821 tctcctgaaa tgcttctgct gcacagggag tttcctgtaa aatctctgtc agttgttctg
     2881 gtgttgcagg ttgttgcgca aggtaaacga tgtaaaaagg aatacataca atcatagcca
     2941 taacgccaac aaatataaaa gccagaacag attttaaata tatcgacttc atttcatttt
     3001 ccttcgaagc agtaagcact cattgcccac caccatttag tgtttccatc acgctgttga
     3061 aaggttcatt tgttttaact tcaatacact cccctgtatt tccaaagccc ctgccgccat
     3121 gtgcttcgat tgtccgaaga acctgcacgc atacgtccgt atttggcatg ggtgtaatat
     3181 tccagcttcc gccaccgtta atatttttta tcagcacaag aaaaataaag gtatgcattc
     3241 tttattatct ccgcttgtat attaatcact gaatatccct tcagttaact tatgaggtca
     3301 taaaacactg cagattttca ttcagattta ctctcttcag caaactttac tctcttcagc
     3361 aaacttccgg atggtatcct gttcacgctg acgggtggca gactcctgca ttgcatccac
     3421 tttctcctgc cagagacgcc ggatatcctt ttcatcccga ccagtcagtt ttttaccgga
     3481 cagcagacgg ttaagcagtt cctgtctcgc atccggtacg tcttcagtca gacgcataag
     3541 ctgaagcagg ctctgacggc tgagaaccat gagtgattcc ggcaggaggg tgaagaagaa
     3601 aataaaaaag cataccgcac cacagcaggc aaggaccgca aacaggattt tcagatatat
     3661 accatcaacg ccgaacgcag caataataca gggaatccat gcaatacacg cacaatacgc
     3721 caggatgtta tctattcggt tatgtctgcg atatatctgt tgatgttcat ccagaataga
     3781 atctattttt tcagcttctg atttatgcat agtgtttcct ctgcgtttcc tgccagagac
     3841 gccggatatc ctgttcatcc cgaccagtca gttttttacc ggacaacaaa cggttaagca
     3901 gttcctgtct cacatctggt acgtcttcag tcagacgcat aagccggagt aattcctgac
     3961 gactgagtac catgcgtgat tcctccggta cgctgtaata attaaaaata cgactgaata
     4021 ccccacaaca gcaggcaacg gacaggcaca gggatttcag atacaggcca gaaacacaga
     4081 atgtggcaac accacaaaga atagctataa ctaaaaatcc cttacccagt gccatacaca
     4141 aagaaccctg ttttctggat atccgctcat gctcatccag aataaaatct attttttcat
     4201 tttctgattt atgcatagtg tttcctctgc ctttcctgat acgcctgaca ttcaacgcac
     4261 aacgtcacac cgggaaatat tttccgccgg gcttccggaa taggatttcc gcatgcttca
     4321 cagagataaa caggaatacc atgaacattt atttcctggc gtatccggtt tattcctgtc
     4381 atggtcagtt gttctgtcac tgaatatgct tcatcggctt catcactcac ggaatattcc
     4441 tcccgacatg ttctgacacg ctaccgccca tcccttttca cgcacagggc actcgtttta
     4501 caggcggatt ttgaatacag aaagcccgcc ggcattgccg gctgatatac aacttcaggg
     4561 ccagttaatt aatacgtggt tttccccacg cagacggttt aataacaaaa aacacgctgg
     4621 atggctgatg ataaacatcc tggttatcaa tataaggcgc tatccataat gctgccgtct
     4681 gctcacccgt tcttagcgga cgaggaggtg ttaccggcgt aactgaactg accggaacaa
     4741 ccgttttcac ttcccgggca gcggtaaaca gcgggcgtgg tgccagcagt ttctgctccg
     4801 gatgtgctgt caccgcaggt ctgctgccgg acggagtggt cgcggtgacg gtctgcaccg
     4861 gcattgtccg gaagttccct tcagccaggc gcggcagtga tgccgcaacc ggctttgctt
     4921 ctgaggactg ctccagtttt ttggccttct cattggcctg ctccatcgtc atacaggtat
     4981 cggatgtggt ggcgttacac tcaaattccg tatttgttcc tgcgcagccg gaaagtaata
     5041 aggttcccag cagaggaata aataaagaaa tctgtttcat tgcccttctc cttttttatt
     5101 actccccaca acagcagaaa ttaactctgc tgtcagattt gttttccctg cacagggatg
     5161 tccggagacc actgtatttc ccggagaaga aaataagctc atcgacattc ccttcttcat
     5221 cacaccgaca gcagccatat taaatttgtg cggcaatgat tcagaactga acaccttcgg
     5281 agcaggaaga actatttttg tggtgcagtt actgagaaca gcatgcataa aatcatctga
     5341 tacgttttta tccggcacaa cgcgggctgt aataacatta atattacgca tgttcatttt
     5401 tatctccgtt gcttctgttt gttcggtcat cagggaaata aacatggcat ttcctgattt
     5461 ttcccggcag ggaagcgtct tccgtataaa cgtccttcag atattcatct gcggttacca
     5521 cccggataca tttcattttt ccgggcagca ccggatattt cccggtgatc gctgccagcg
     5581 agacatacat cagtgccagt gttgcccata atgcggtgac cagttgcatc agtgaccggt
     5641 cagtctccct gacataatct gcagcatcag ccgcgcggta atggcagata tcctgtattt
     5701 ctgatctgcc tgaagaatca gaggaagaaa accagcattc actgaccggg aactgtccgg
     5761 caggtgctgt ttgtcttacg aagttaatct ccttaaccag taatttcccc ggcgtggtgt
     5821 tgtccttcca taaaacaaac agagccatca gggccattgt ggccagtggc agaatgacgg
     5881 tgtacttcag acaccagaac agtcctcgca gaacccgggc gacaacatac cgggcggcat
     5941 ggcacgcctg acgggacgac atattatttg ccatcgttgc ctcctccctg gctgccggac
     6001 ataaactgct gcggattcag gttgcccagc tgcttcagca tctggtcagt attaaagccg
     6061 ttaagatgac tttcagctga tggcggaaca ggaaccggac tttccggatt atcttcttcc
     6121 gctctgttct gcgtgcgctc cagcgccatc tcttccacgg ttttcagctg gaagccgtcc
     6181 tggaacacca cggtcacttc gttgcccgca ccaatcggta tcaccgggtg atactgttcg
     6241 gcacgtttga tgtagtagtc actgagcgtc tgtgcggctt tcgatgtgcc gccaccgata
     6301 cccattttca ggacatcacc agccccgtaa gcggctgtgg cacccagccc gacagccggc
     6361 tgggaggcgc gctccattcc ctgaccgata ccgtcaacaa atcccgcgcc ccatgcccag
     6421 ccgaggattt tgccgttacg catcaccact tcgcccttga taccgttttt accccggaag
     6481 ctgacatgcc ccttaatcgg catatcaata gttttgccgt ccttcaggca actgatattg
     6541 cgggtgcgta caatggcacg ctcactggac acatcccccc aggcttccag ccccacaaaa
     6601 catcccgttg cgtcatacgt cttgctgttc ggcatttcca ccaggccggt gatacgcaac
     6661 tgcatcggca ccgtggattc attaccggtg actgaggcat tggcatccgc cccttcaatc
     6721 agcatggctt tcgcaaaact tcctgacgga atgtacggca gcgatggtcc ctgttttccc
     6781 tcattacggg taaacacctt acgctgtatc cggttaggca ccggcacaga ctggtacgtc
     6841 acctgtggtg gtggcgtgac accattcccg ggataaaacg ccgtcggcgg tggaacagca
     6901 acgctgccct gcggcgggaa ggatacgggg gtgtttcctg gctgtggttc gccttccggc
     6961 ccgggcggtg aggcaggcat ctgaggtaca ggctcaccgg tcgccgtgac gggattagca
     7021 cccagggctt ttacctgctc tgccagggcg gcattgtcct gtcccagctt ttcaatacga
     7081 cgctggtcat caccgcgctg tttgttcagg acatccagtt cacgacggat ttcctcatac
     7141 tgcttctgca tctgcgctgc cgtcacctgc atctctgtgg tggcatgctg acgcaccttg
     7201 tcatcaaagg tcgtatccac cacacctgtc atatccggca caggctcctg ttcagccacc
     7261 gcctcaccgt taccggacat gtccacatca gacagataca gtaccccacc aatcgcggag
     7321 gctgcaccga caaccacaat ccccagccac aggtactgct tgcgtttcac aatggtattg
     7381 atactggcca tcagttgccc tccccgtcac ggatgacata cacatccatg cgcgcacctg
     7441 ccagtaactg gctggcaggc tggctgaaca tcacggcacg ggttcccggc tgccagaagt
     7501 cactttcccg gatattcagg gcagacagcg ttttattttc cacggcaaag cggaccatct
     7561 tcaggtgatt ccccgtccat acggcatctg ccactgaaga cagccccgcc ggggcctgca
     7621 gggtttcctt tgtcacagga acctgatacc agcctgcagg taattttccg ccacggacag
     7681 cctggctgat ggtcacaagc agggattcgt aaggcgtgga cgtttcccac gctccggctt
     7741 cttctccggt tccgcgcagg tcactgacca gctgaatggt acgccccgcg ccttcacggg
     7801 gaacagcctg aatggaaagg ttcagaccac gctctgtttc cagaatgaac gtaaagggct
     7861 ttttgttgac ggtggccacc accacaccgc cggaggcggt ctgctcatta ttggtcaggg
     7921 caccatccag actgttcacg gcgataatcc ggtcaccggg aacggtaaac agattcgggc
     7981 tggtattgct gatggcaata ctggcctgac caccgttcac cattggcacc acagtggggg
     8041 ccagcgtacc gtttgcggcc atcacgctgc aggaaaaaaa caggccgccg aatattattg
     8101 ccgtattatt ttttctcatc gtctgtttcc ccgaaattat ccagccaggt caccccgttt
     8161 tcccgcttca gaataaggat gtaatgtttg atatcagtaa aaggttcgga gttaccaatc
     8221 catgttttcc gaattccacg gatttccaca cgaccatact gaggccagac acggacactg
     8281 gtctggaaaa aagcagaatt cacgttatct tttttaatga gtttcgcttc ttcagccaga
     8341 ataactttca tctggttctg tgcacccggg cggatatatt gcagaagcgc ctgatgtgaa
     8401 gcatcgacga tttccggtga gacattcaga cggagggcaa taaatgacag cgccatctgc
     8461 tgcaaataag aggcatcggc actgtt
//
LOCUS       DAZOXR010000175         8473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZOXR010000175 DAZOXR010000000
VERSION     DAZOXR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.181"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(270..1013)
                     /locus_tag="ACHUNN_003537"
     CDS             complement(270..1013)
                     /locus_tag="ACHUNN_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0577816.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHUNN_003538"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHUNN_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR0577817.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHUNN_003539"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHUNN_003539"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR0577818.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHUNN_003540"
     CDS             1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHUNN_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0577819.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="ACHUNN_003541"
     CDS             complement(2637..3404)
                     /locus_tag="ACHUNN_003541"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0577820.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHUNN_003542"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHUNN_003542"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0577821.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="ACHUNN_003543"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="ACHUNN_003543"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0577822.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHUNN_003544"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHUNN_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0577823.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="ACHUNN_003545"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="ACHUNN_003545"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0577824.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVAWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>8473)
                     /locus_tag="ACHUNN_003546"
     CDS             complement(8354..>8473)
                     /locus_tag="ACHUNN_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0577825.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2044 a         2124 c         2229 g         2076 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtggc atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agc
//
LOCUS       DAZOXR010000176         8458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZOXR010000176 DAZOXR010000000
VERSION     DAZOXR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.182"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..395)
                     /locus_tag="ACHUNN_003547"
     CDS             complement(<1..395)
                     /locus_tag="ACHUNN_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577826.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINENFVGMDFTCEFRPEKMTNLINIKN"
     gene            complement(684..947)
                     /gene="cedA"
                     /locus_tag="ACHUNN_003548"
     CDS             complement(684..947)
                     /gene="cedA"
                     /locus_tag="ACHUNN_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0577827.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            1130..3391
                     /gene="katE"
                     /locus_tag="ACHUNN_003549"
     CDS             1130..3391
                     /gene="katE"
                     /locus_tag="ACHUNN_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0577828.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3649..4407)
                     /gene="chbG"
                     /locus_tag="ACHUNN_003550"
     CDS             complement(3649..4407)
                     /gene="chbG"
                     /locus_tag="ACHUNN_003550"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0577829.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4420..5772)
                     /gene="celF"
                     /locus_tag="ACHUNN_003551"
                     /gene_synonym="chbF"
     CDS             complement(4420..5772)
                     /gene="celF"
                     /locus_tag="ACHUNN_003551"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0577830.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5877..6719)
                     /gene="chbR"
                     /locus_tag="ACHUNN_003552"
     CDS             complement(5877..6719)
                     /gene="chbR"
                     /locus_tag="ACHUNN_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR0577831.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6727..7077)
                     /gene="chbA"
                     /locus_tag="ACHUNN_003553"
     CDS             complement(6727..7077)
                     /gene="chbA"
                     /locus_tag="ACHUNN_003553"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0577832.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(7128..>8458)
                     /gene="chbC"
                     /locus_tag="ACHUNN_003554"
     CDS             complement(7128..>8458)
                     /gene="chbC"
                     /locus_tag="ACHUNN_003554"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR0577833.1"
                     /translation="VLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNVFLS
                     FGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKV
                     DALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVAEMFTFIVRRN
                     WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLAS
                     LGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAA
                     GKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEP
                     ILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNT
                     NGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANALKF"
BASE COUNT         2308 a         2179 c         1852 g         2119 t
ORIGIN      
        1 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
       61 atcccaacaa aattctcatt aatatctctt atccgaaact cactgccata aagattatta
      121 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggagct
      181 cctggctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
      241 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
      301 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
      361 ccataattac tctgtattaa gtttgtagaa aacatctccc gccccatatt gttaacaaaa
      421 ttattatctc atttaaatct aagtcactta caatataagt ttaagagcga cgccacagga
      481 tgaactatca aaaatagctc atcatgatta gcaaaactta accattttaa aataaataaa
      541 caattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
      601 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcatg taactcccgg
      661 caaaaccggg aagtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
      721 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
      781 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
      841 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
      901 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
      961 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     1021 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     1081 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
     1141 taacgaaaag aacctacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     1201 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     1261 gccaggtgca caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa
     1321 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
     1381 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
     1441 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
     1501 acgtattgtt catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt
     1561 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
     1621 taggttctct accgttcagg gtggcgctgg ctctgcagat accgtgcgtg atatccgtgg
     1681 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
     1741 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
     1801 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
     1861 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
     1921 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
     1981 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
     2041 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
     2101 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
     2161 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
     2221 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
     2281 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
     2341 cgatccgctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
     2401 tgggccgaat ttccatgaga ttccgattaa ccgtccgacc tgcccttacc acaacttcca
     2461 gcgtgatggc atgcatcgta tggggatcga cactaacccg gcgaattacg aaccgaactc
     2521 gatcaacgat aactggccgc gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc
     2581 atatcaggaa cgcgtggaag gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata
     2641 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
     2701 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
     2761 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
     2821 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
     2881 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
     2941 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccctgaa
     3001 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
     3061 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
     3121 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
     3181 cctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
     3241 taaagcaaca atcaaggtcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc
     3301 tgacggtagt tttatggatg aactgctaac gctgatggca gcacaccgcg tgtggtcacg
     3361 cattcctaag attgacaaaa ttcctgcctg atgggaggcg gcgcaattgc gccgcctcaa
     3421 atgatttaca tagtgcgctt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta
     3481 caaaactgtg caaattcaat atattgcagg aaacacgtag gcctgataag cgaagcgcat
     3541 caggcagttt tgcgtttgtc agcagtctca agcggcgcag ttacgccgcc tttgtaggaa
     3601 ttaatcgccg gatgcaaggt tcacgccgca tctggcaaac atcctcactt acacatcccg
     3661 ataactcccc aaccgataac cacgctgagc gatagcacct ttcaacgacg ctgatgtcaa
     3721 cacatccagc tccgttaagc gtgggaaaca gtaagcactc tgacggatag tattatcgat
     3781 aaacgccgga tgacacatca cctccagcga acgatcaccc cgatggcttg cgtcatccag
     3841 cacttgcagg aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccctg
     3901 agaactgcgc agattagccg gtaaatcacc agagttagat agcggctgac ggtcgatgcg
     3961 caacgcaatc ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat
     4021 atgcacatga tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg
     4081 caaatactga ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc
     4141 caactgccag atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag
     4201 tggcttgccc atagtaagga caaagtacat ccctatggcc agactcggtt catcacgact
     4261 caactgcacc gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaat
     4321 cccattgcga caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc
     4381 ggcattaaca atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc
     4441 tgcgatgcag tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc
     4501 cagcagctca gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac
     4561 atcgttaaat tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt
     4621 gtgaatcagc cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc
     4681 atcgcgcccc agcttacagg tcatttctac cgcccagtct gccggaatat tatcaatatg
     4741 cccatgatgc gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat
     4801 cacttcgcac gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt
     4861 aactttcaac tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac
     4921 ctgcgctcgt gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg
     4981 cttgaagtaa taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc
     5041 gctaaatggc agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac
     5101 accatcaagc aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac
     5161 catatggttg aggccgaaca aatcgataga taaatcatcg ctgtctttca gcatcagaac
     5221 atcgtgaata aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa
     5281 tccggtatga cgataaacgg cttcagtaac cattcccgcc gggttagtga agttaatcac
     5341 ccatgcattc ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt
     5401 acgcagacct ttaaacagac cgcccgcgcc gttggtttcc tggccaagat aaccatgact
     5461 taatggaata cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt
     5521 aacgaaatca gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg
     5581 gacgccagcg ttatcaatca tccgttggca gagttcaaaa atgatatcca gtttctcttt
     5641 accatcttcg acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat
     5701 aaatccttcc agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa
     5761 tttctggctc ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc
     5821 ccggacataa ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac
     5881 tgattaaatt cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta
     5941 aacgttttaa taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcacg
     6001 gaatagttgg tcatttccag ttgttttttg gcaaaattaa tacggatctc attaataatc
     6061 tgcattggtg ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg
     6121 gctgacaacg tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatc
     6181 ttttctaccg tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa
     6241 tgacgtaaac ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt
     6301 tcgcggaaat tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc
     6361 cggtatacct gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa
     6421 aagcgtttac tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga
     6481 tggtgcgaac ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg
     6541 ttaatctcct ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga
     6601 tgcagcccgc tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta
     6661 aacaactgct gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc
     6721 gactccttat gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc
     6781 aagcatggac gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt
     6841 accttcgccc gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc
     6901 catccgtgac tgatccatca tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt
     6961 cagtgccgca tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac
     7021 ttcttccagc tcttcagctt ccgtttgcgt atcgggaatg ttatcgagat ccatcataca
     7081 tcgttcctct ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg
     7141 gcgttagcga tatcttcttc gcgctcttct ttatcaatcg cgttttgtgc tttgttagcc
     7201 accacaacaa agggcagata aattaacgtt gcgataccaa ggttgaagag tgcaaccagc
     7261 aatgcggcga cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac
     7321 ggtgcaatat tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc
     7381 gccagaatcg gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc
     7441 agaccaaaca gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc
     7501 acctgacgat aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc
     7561 gcaccactgc ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag
     7621 gtcttaccgg ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt
     7681 tccagcgccc acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga
     7741 ataccgaaga accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc
     7801 aacgatgcca gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag
     7861 gtattcaacg cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat
     7921 gccgagaagg aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg
     7981 cggcggacaa taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg
     8041 atattcgctc cacctaacca gtttgcgcca accacatagg cctcaccgac actatatggg
     8101 gtgacggtca taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc
     8161 tcttctgcca gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt
     8221 ccgttatata cgttgccgcc aatacctttc aggccattaa gtgtttcaat ggttgaggcg
     8281 tcgaggcgaa tacctaagga ataaaaaaac gccccctccc caaagcttag aaaaacgttg
     8341 ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt
     8401 ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag gagtacct
//
LOCUS       DAZOXR010000177         8421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZOXR010000177 DAZOXR010000000
VERSION     DAZOXR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.183"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            153..1931
                     /locus_tag="ACHUNN_003555"
     CDS             153..1931
                     /locus_tag="ACHUNN_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR0577834.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1971..2450)
                     /gene="xdhC"
                     /locus_tag="ACHUNN_003556"
     CDS             complement(1971..2450)
                     /gene="xdhC"
                     /locus_tag="ACHUNN_003556"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0577835.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2447..3325)
                     /gene="xdhB"
                     /locus_tag="ACHUNN_003557"
     CDS             complement(2447..3325)
                     /gene="xdhB"
                     /locus_tag="ACHUNN_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0577836.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3336..5633)
                     /gene="xdhA"
                     /locus_tag="ACHUNN_003558"
     CDS             complement(3336..5633)
                     /gene="xdhA"
                     /locus_tag="ACHUNN_003558"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR0577837.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6048..6803
                     /gene="actS"
                     /locus_tag="ACHUNN_003559"
     CDS             6048..6803
                     /gene="actS"
                     /locus_tag="ACHUNN_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0577838.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6882..6951
                     /locus_tag="ACHUNN_003560"
     tRNA            6882..6951
                     /locus_tag="ACHUNN_003560"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6914..6916,aa:Gly,seq:ccc)
     gene            7156..>8421
                     /locus_tag="ACHUNN_003561"
     CDS             7156..>8421
                     /locus_tag="ACHUNN_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR0577839.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
BASE COUNT         2223 a         1943 c         2099 g         2156 t
ORIGIN      
        1 aaatgtgata tctcttccat aattgcatta gaaaatgata aaaatcttat gtttttatca
       61 aagccaattt cgcctgagtt aatatcatca tgatctgtga accatcaacg tcttcaatga
      121 tcgactttta agtaagtctg gaaggtaaag ctatggagct tgctactacg cagtcagtat
      181 tgatgcaaat tcaaccgaca attcagcgtt ttgccagaat gcttgccagc gttttgcagt
      241 ttgaggttga gatcgttgat gaaaacttgt gtcgcgtcgc cggaacgggc gcgtatggga
      301 agtttcttgg tcgccagttg agcggcaact cacgcctgct ccgccacgtc ctggaaacga
      361 aaactgaaaa agttgtgaca cagtctcgct tcgatcccct ttgcgaaggt tgcgatagta
      421 aagaaaattg ccgcgaaaaa gcatttctgg gtacgcctgt cattttacag gatcgttgtg
      481 ttggggtgat aagtttgatt gccgttaccc acgagcaaca agagcatatc agtgataatt
      541 tacgcgaatt ttctgattat gttcgccata tatccaccat ttttgtttcg aaacttctgg
      601 aggatcaggg gccaggagat aacatcagta aaatattcgc gaccatgatc gataatatgg
      661 atcagggcgt attagttgtt gatgatgaaa gtcgggttca gtttgttaat cagactgcct
      721 taaaaacact tggtgttgta caaaataata ttattgggaa acctatccgt ttcagaccat
      781 taacatttga gagtaatttt actcatggac atatgcagca tattgtttcg tgggacgata
      841 aaagtgaatt aatcattggt caattgcata acattcaggg ccgacaatta tttttaatgg
      901 catttcacca atcgcatacc agtttttctg tagcaaatgc acctgatgaa ccacatattg
      961 aacaattggt tggcgagtgc cgtgttatgc ggcaattaaa acgactcatt agccgtattg
     1021 cacccagccc atccagcgtt atggtggttg gtgaaagcgg cacgggtaaa gaagtcgtcg
     1081 cccgagcaat ccataagttg agcggaagac ggaataaacc ctttattgct atcaactgtg
     1141 ccgcgattcc ggagcagctt ctggaaagcg aactgttcgg ttatgttaaa ggcgcattta
     1201 ctggtgcttc tgccaacggt aaaacagggt tgattcaggc ggcgaatacg ggcacgctgt
     1261 ttctcgatga aataggtgat atgccattaa tgttgcaggc taaattactg cgcgctattg
     1321 aggcgcgtga aattctgccg attggtgcca gtagcccaat acaagtcgac attcgcatca
     1381 tttctgcaac taatcagaat ttggcccagt tcattgccga aggtaaattc cgcgaagatc
     1441 tcttctaccg acttaatgtt atcccgataa ctctgccacc gctgcgtgaa cgtcaggaag
     1501 atattgaact attggtgcat tactttttac atctgcatac ccgtcgtctg ggatcggttt
     1561 atcctggcat tgctcccgat gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc
     1621 tgcgcgagtt aagcaatttg atggaatatc tggttaacgt ggttccttca ggtgaagtta
     1681 tcgacagcac gctattgccg ccaaatctgc tgaataatgg cacaacggag caaagtgatg
     1741 taacagaggt cagtgaggcg cacctgtcac tcgatgatgc gggcggcacg gcgctggagg
     1801 agatggaaaa gcaaatgatc cgcgaggcgc tttcacgtca taacagcaag aagcaagttg
     1861 ctgatgaact gggcatcggc attgctacgc tctatcgcaa gattaagaaa tatgagttgt
     1921 taaacacata acctcagtga atccggcctg aattcaggcc ggatatcctt ttacttcgtt
     1981 ttctcgcaat ccagaactgt atttacaatc atctgatacc ccgtgcagcg acaaagattt
     2041 cccgccagtc cgcgacgaat ttccgtaatg gttaatggct tctcgcgtgg tttcgccagc
     2101 attgccgtgg tagccataat caggccaggc gtacaaaacc cgcactgcac tgcgccggat
     2161 ttcgcataag cctgctgaac atgagaaagt tttccgcctt tcgcttcacc ttccagcgtg
     2221 cggatctcct ttccttcagc ccaggcggca aggtataagc aactgtctat tgctgtgccg
     2281 tcgaccaaca ccgtacaggc accacattca cccacgcagc acccttgttt gacacttagc
     2341 agtccttgtt cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg aagctgaaaa
     2401 ggcatcccgt taatggtgca ttcgatggta attgtttcgc tgtgattcat tgcaattttc
     2461 cccccgccgc ggcgacggct tcgctaatca cttttttggt catcgtctgg atgagatgca
     2521 gacgaaactc tttactggcc cgccatgaag aacgcggggc gacatcttgc aggacagatt
     2581 cgctgatagc ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc tgttcggcat
     2641 gttggcagcg aatcggcgtt ggcgcggcaa caccaaatgc caggcgtaat tcgctgaaat
     2701 tgccgttatc cagtcggcaa tgtgcggcgc agccaatcgt tgaaatatcc attgcgtcgc
     2761 gcatggcata tttaaaatgc gcgctgcccg cgtgttcttt cggctgtggc ggaaaatgaa
     2821 aggcgacgag gatttcgtca tgctcaagag acactttgcc cggcccggtg tgaaagccat
     2881 taatcgggac gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg tcataaatta
     2941 gcgttggcgt ggcagaatct gcgctggtgg caccgttgca aatatttcca ccgtaggtag
     3001 cgacgttacg gatctgcggt ccagcaatgg acgtggccgc agcacataac gccgggagat
     3061 gacgttgagt tataggagct tctattagct gggtaaatgt cgttgcagag ccgatacgta
     3121 gcgagccatc ttccgccagc gtaattcccc gcagctccgc cagattatga atatcaacaa
     3181 tatggcgata acggtcattg tgatggtgga gctgaatcag tacgtcagtg ccatcggcga
     3241 gcagtttggc ctgcgggttg tcagccagca ggttgatggc atcggcaagg gttgctgcgc
     3301 gatggtaaga agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc cagatggaac
     3361 tcttcatata accgttttgg cgtcagcggc agtgtattga ttgcaacacc ggtagccatc
     3421 ttcaccgcgt tacgaatagc agcggcaaca ggaattattg gtggctcacc cagtgactta
     3481 tgtccgtatg cggattgcgg ctcattgatt tcgacgaacg cgctttccag ttgtggcaga
     3541 tccggcatgg tcggcatttt gtaatccagc agattggggt tacggaccac gccgctttta
     3601 gcatcgatga tcatctcttc aaatagcgcc cagccaatgc ccattcccat tccgccgtgt
     3661 acctgacctt ctgccagcag tggattaaga atatgccctg aatcatgaac gttgaggatg
     3721 cggttgatgg tgactttgca cagcgcaata tcgaccgtca gatcaacaaa ggtacagcca
     3781 aacgccgatg ggttagtggt ggttttgatg gagctttcag cagagagctg cccgccgcgt
     3841 tcagggtggt agaaagcgtc catcgccaaa tcttttaacg acattaacgg ctcttccggt
     3901 cgttcaacca gcacgatatg gccttttatc agggtcagat tcatcgctga ctgatgtagc
     3961 atgactgcgg cgtgagcgat gattttctct tttaataata gtgccgcact gcgcagcgca
     4021 ggcgcggcaa catagctctg gcgtgaggca aatgcgccgg gagcgaacgg cgtaacgtcg
     4081 gtatcttgag ttgaaataac gcgaacgtcg ctgaccggaa cccccacggt ttctgccacc
     4141 atttgcgaga agacggtgtc ggcaccctga ccgatttccg tcgcgccgct ttgcacgttg
     4201 atggttccat cctgattcat cagaaggcgc gcgcctgcta tttctacgcc gacaggccag
     4261 gtgttagagg tatagctaaa acaggcgacg ccaacgccgc ggcgcaaatt gccttgctgg
     4321 ttctggcatt ctgcacggcg tttttcccat tcaaagattt tccggccttt ttcaagacac
     4381 tccggcaacc ctgcgctgta aatacgtttg cccgtgagcg gattagcatc tccttcgcgg
     4441 gcggcgttgc gtaaacgaat ttcaacagga tcaataccta acgctgtcgc ggcgtcatca
     4501 agcatagact caacggcaaa tacgacttgt ggcgcgccat aaccacgcat cgcaccagcc
     4561 gaggggaggt tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg aggataaagg
     4621 taagcgactt tattcctccc cgcagaagcg atggagtgcc cgtgagatgc ataagcgccg
     4681 gtgttagaca gaacatccag actataacct ttcaatgttc cgtcgcggtt cacgcccatt
     4741 tgcccgtcaa tggtaaaagc gtggcgggta cgggttgcga ggaaacactc ttcacggcta
     4801 agggaaactt tcaccggaat gccgccaagc ttgctggtca ggaatgctgc cattggctct
     4861 tccagtacat cctgtttatt accaaaaccg ccaccgacaa acggtttgat gactcgtacg
     4921 catgaccagg gaatatccag cgcctgacca accacgcggc gaacaatgtg cgggatctgg
     4981 gtgctggaaa cgatggtaat tcgcgagtca tcctccatcc acgccagcga tgttacgctt
     5041 tccatgtgac aatgttgaat aacgggggtc tgatagtgcc cctgtacctg gtagtcggcg
     5101 gcatcgattg tttgttggac attacccgtc gacatcgtgc tttgtttcag taaattgcca
     5161 ccgttatgga ttggtgcagc gtcttctgcc agcgccgctt ctggcgtggt gataacgggt
     5221 aattcttgcc actcaatgct gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg
     5281 gccacgacga tggcaacggc gtcaccatga tgacgaacat ggcgagttag cagtgcgcga
     5341 tcggcggtat cgcgcttgtt ttcgtcaagt gtccaggcat gcccagctgt agcgaatgga
     5401 atatcaggca catcttccca ggtaaaaatc gccagtacgc ccggcaaact tctggcttgt
     5461 tcatcattaa tacttacggc ataaccatgt gcgatagggc tacgtacata tttcgcataa
     5521 cacatgcccg ccataacata atcgtcagta tatcgtgccc gcccggtgac cttagcaatg
     5581 gcatcgacgc gcatgcatga ttcacccgta gcggttgctt cccgcgcttc catagaaatc
     5641 ccctcgattt gaacatttgg ttcttatggc aaagatttag tctaagcagg caagatttac
     5701 gccagaaacg acgataagcg ttaggggagg taggaagtgt gatgacgtta aaaaaacaca
     5761 gatatgatac gaaacaggac tctgaatagg ggcagtgata cgtatcaata tgagtggcga
     5821 taaaatcaat gtgataaatt gggattatta cgcatcagga tgggaaatag ggtaaaaagt
     5881 caggaagttc gatacctcta cagcgaatat tttgctgatg atacttccct ttgccaaatg
     5941 aaattaacct ctttctctat taccacgttt ttccagaagc aagagattgg gtatcaagag
     6001 gctggctgct atgataaggc gtcttgtttt ttaagcgagg aaagattttg agtgcgggac
     6061 gcctgaataa aaaatctctg ggtatcgtga tgttgttatc ggttggactg cttttggcgg
     6121 gctgttcggg tagcaaatca tccgatacag gaacgtattc cggctccgtt tacaccgtga
     6181 aacgggggga tacgctatat cgtatttcgc gcaccacggg aaccagcgta aaagaactgg
     6241 cgcgactgaa cggcatttcc cccccttaca ccattgaagt tggtcagaaa ctaaaactgg
     6301 gtggggcgaa aagtagcagt agtacacgta aatcaaccgc caaatcaacg accaaaaccg
     6361 catcggttac accgtcatca gcggtaccga aatcatcctg gccgccagta gggcaacgtt
     6421 gttggttatg gccaacgaca gggaaagtta tcatgccgta ttcgacagca gatggcggca
     6481 ataaagggat tgatatctca gctccacggg gtacacctat ttacgccgcg ggtgcaggaa
     6541 aggtggtgta tgtgggcaac cagctgcgtg gctacggtaa tctcatcatg attaaacaca
     6601 gtgaagatta cattacggct tacgcccata atgacacgat gctggtaaat aatgggcaaa
     6661 gcgtgaaggc tgggcaaaaa atcgccacta tggggagcac ggatgcggca tctgttcgcc
     6721 tgcatttcca gattcgttac cgtgcaacgg caattgatcc gctacgttac ttgccgccgc
     6781 agggcagcaa gccaaaatgc tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc
     6841 taaggagagc gtaaggttta taatgcctta cgcatctcga agcgggcgta gttcaatggt
     6901 agaacgagag cttcccaagc tctatacgag ggttcgattc ccttcgccgc ttttgctgta
     6961 tctgattttc tatattatct taattcaatc gcttcagact tttcgcaatc gccgtaatat
     7021 ttccccattt tgttgtaaat aaaaagcgtt tacgcaatgc aatccttttg taacaaccca
     7081 ggtaaaacac cttattttca agttgttaaa taagtggtta tttacgcttt taacaaacga
     7141 ttgatggaat attttatgaa agggaaatct gcattaactc ttctgctcgc tggtatattt
     7201 tcgtgcggca catgccaggc gacaggggca gaagtgacga gtgaatctgt atttaacatt
     7261 cttaattcca cgggggcagc aacagataaa agttatttat cgttaaatcc agataagtac
     7321 cctaattatc gtcttctgat tcactcagcc aaattaaaaa atgaaataaa gagccactat
     7381 accaaagacg aaattcaagg gttgttaaca ttaacagaaa acacacgaaa gttaacgttg
     7441 acggagaaac cctggggaac atttattctt gcttctactt tcgaagatga caaaacagcg
     7501 gcagaaaccc actatgatgc tgtctggctg agggatagtt tatggggcta tatggcatta
     7561 gtatcagatc agggaaatag tgtagccgca aaaaaggttc tgctcaccct gtgggattat
     7621 atgtctacgc tcgaccagat taagcggatg caggacgtca ttagcaaccc caaacgactg
     7681 gatggagttc ccgggcaaat gaatgctgtg catatccgat ttgacagcaa ctcccccgtg
     7741 atggcagatg tgcaggaaga aggcaagccg cagctgtgga accataaaca aaatgatgcg
     7801 ttaggtcttt atcttgatct ccttatacaa gcaatcgaca ctggtacaat taacgctgaa
     7861 gattggcaaa aaggtgaccg attaaaatcg gttgcgctgc tgattgctta tctggataaa
     7921 gccaatttct atgttatgga agattccgga gcatgggaag aagatgcccg attaaacaca
     7981 tcttccgttg cactggtgac ctctggactg gaacgtttgt cgaacttact ctcgaagaaa
     8041 gactctgttt ttgtctctga tttactacgc gaagctaaag ccaatgaact ggatgaacct
     8101 ctttcaacaa cgaggttgaa ccacctcata gataaaggtt atgaaagaat aacgttacag
     8161 cttgatttag ggggagaatc cccaggttat ctggaaaaag ataaacatta cagagaagcc
     8221 gatgcggctt tacttaatgt tatttatcct gcaaatcttg caaagataaa caccagaaga
     8281 aaagagcagg ttctaaaaat agttaaaaaa ctagcaggtc cgtatggtat aaaaagatac
     8341 gagaaggata attatcagtc ggcaaatttt tggttcaatg atattaaaac cgataccgat
     8401 caaaattcac atgcgaaaag a
//
LOCUS       DAZOXR010000178         8401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZOXR010000178 DAZOXR010000000
VERSION     DAZOXR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.184"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUNN_003562"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHUNN_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0577840.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHUNN_003563"
     CDS             complement(1360..1518)
                     /locus_tag="ACHUNN_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0577841.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHUNN_003564"
     CDS             2089..2238
                     /locus_tag="ACHUNN_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577842.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="ACHUNN_003565"
     CDS             complement(3005..3445)
                     /locus_tag="ACHUNN_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0577843.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="ACHUNN_003566"
     CDS             3663..4052
                     /locus_tag="ACHUNN_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577844.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4284
                     /locus_tag="ACHUNN_003567"
                     /pseudo
     CDS             4096..4284
                     /locus_tag="ACHUNN_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHUNN_003568"
     CDS             complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHUNN_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0577845.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHUNN_003569"
     CDS             5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHUNN_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0577846.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6238..6480)
                     /locus_tag="ACHUNN_003570"
     CDS             complement(6238..6480)
                     /locus_tag="ACHUNN_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR0577847.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6786..7622)
                     /locus_tag="ACHUNN_003571"
     CDS             complement(6786..7622)
                     /locus_tag="ACHUNN_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0577848.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7622..>8401)
                     /locus_tag="ACHUNN_003572"
                     /note="aph(3'')-Ib"
     CDS             complement(7622..>8401)
                     /locus_tag="ACHUNN_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0577849.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1889 a         2322 c         2332 g         1858 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag
     4321 agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc
     4381 gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct
     4441 tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc
     4501 attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc
     4561 gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc
     4621 ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga
     4681 atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg
     4741 ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc
     4801 gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg
     4861 agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag
     4921 cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag
     4981 aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg
     5041 ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg
     5101 ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc
     5161 caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc cagcgagacg
     5221 agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca
     5281 aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag
     5341 tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg
     5401 tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac gtctggcctc
     5461 cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg
     5521 gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag
     5581 tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca
     5641 aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc
     5701 gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc
     5761 cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg
     5821 cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc
     5881 agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg
     5941 acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc
     6001 aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc
     6061 cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc
     6121 agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg
     6181 aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta
     6241 cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca
     6301 gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc
     6361 ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt
     6421 ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac
     6481 gttctgcctt gcgcagatga gatagatcta gcgtggactc aaggctctcg cgaatggctc
     6541 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     6601 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     6661 cgtcgctacg gggcttccag gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     6721 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     6781 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     6841 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     6901 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     6961 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     7021 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     7081 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     7141 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     7201 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     7261 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     7321 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     7381 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     7441 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     7501 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     7561 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     7621 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     7681 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     7741 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     7801 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     7861 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     7921 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     7981 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     8041 ttcgcgacag cctgcgctca aactgacatt gatcaaccga taggctgtga acagcgccaa
     8101 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     8161 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     8221 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     8281 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     8341 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     8401 c
//
LOCUS       DAZOXR010000179         8392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZOXR010000179 DAZOXR010000000
VERSION     DAZOXR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.185"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            108..719
                     /gene="ruvA"
                     /locus_tag="ACHUNN_003573"
     CDS             108..719
                     /gene="ruvA"
                     /locus_tag="ACHUNN_003573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0577850.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            728..1738
                     /gene="ruvB"
                     /locus_tag="ACHUNN_003574"
     CDS             728..1738
                     /gene="ruvB"
                     /locus_tag="ACHUNN_003574"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0577851.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1885..2670)
                     /gene="znuB"
                     /locus_tag="ACHUNN_003575"
     CDS             complement(1885..2670)
                     /gene="znuB"
                     /locus_tag="ACHUNN_003575"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0577852.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2667..3422)
                     /gene="znuC"
                     /locus_tag="ACHUNN_003576"
     CDS             complement(2667..3422)
                     /gene="znuC"
                     /locus_tag="ACHUNN_003576"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0577853.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3501..4433
                     /gene="znuA"
                     /locus_tag="ACHUNN_003577"
     CDS             3501..4433
                     /gene="znuA"
                     /locus_tag="ACHUNN_003577"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0577854.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4449..5771
                     /gene="mepM"
                     /locus_tag="ACHUNN_003578"
     CDS             4449..5771
                     /gene="mepM"
                     /locus_tag="ACHUNN_003578"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0577855.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5891..6862
                     /gene="lpxM"
                     /locus_tag="ACHUNN_003579"
                     /gene_synonym="msbB"
     CDS             5891..6862
                     /gene="lpxM"
                     /locus_tag="ACHUNN_003579"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0577856.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6950..8392)
                     /locus_tag="ACHUNN_003580"
                     /pseudo
     CDS             complement(6950..8392)
                     /locus_tag="ACHUNN_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2254 a         1981 c         2085 g         2072 t
ORIGIN      
        1 cagttcatcg agacacctcg caagttttct tcatccttcg ctggatatct atccagcatt
       61 tttttatcat acagcattat ctttgattca ttacgcagga gcgtcatgtg ataggcagac
      121 tcagaggcat catcattgaa aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag
      181 gctatgaagt gcatatgccg atgacctgtt tttatgaact ccctgaagcg ggtcaggaag
      241 cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca actgctgtac ggttttaaca
      301 ataaacaaga gcgcacgttg tttaaagagt taatcaaaac caacggcgtc ggcccgaagt
      361 tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc gttgaacgtg
      421 aagaagtggg agcactggtg aaactgccgg gcattggtaa aaaaaccgcc gagcgtttaa
      481 ttgtcgaaat gaaagaccga tttaaaggtt tgcacggcga tctctttacg ccagccgccg
      541 atctggtgct cacgtcgcct gccagcccgg caaccgacga tgctgaacag gaagcggttg
      601 ccgcgctggt ggcgctgggc tataaaccac aagaagcaag ccgcatggtg agcaaaatcg
      661 ctcgcactga cgccagcagt gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag
      721 gtaaaggatg attgaagcag accgtctgat ttctgccggt accactttgc cggaagatgg
      781 ggcagatcgc gccattcgcc ctaaattact ggaagagtat gttggtcagc cgcaggttcg
      841 ttcacagatg gagattttca tcaaagcagc gaaactgcgc ggcgatgccc tcgatcatct
      901 gttgattttt ggtcctccgg ggttgggtaa aactacgctt gccaacattg tcgccaatga
      961 aatgggcgtt aatttacgca cgacttctgg tccggtgctg gaaaaggcgg gcgatctggc
     1021 agcgatgctc actaaccttg aaccgcatga cgtgctgttt attgatgaga tccaccgtct
     1081 atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa gactaccaac tggatatcat
     1141 gattggtgaa ggtccggcgg cacgctccat taaaattgat ttgccgccgt ttaccctgat
     1201 tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt
     1261 gcaacgtctg gagttttatc aggtgccgga tctgcaatat atcgtcagtc gcagcgcacg
     1321 ctttatgggg cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc gcgctcgcgg
     1381 tacgccacgt attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca
     1441 cgatggcacc atctcggcag atatcgctgc tcaggcgctg gatatgttga atgtcgatgc
     1501 tgaaggtttc gattatatgg accgcaaatt gttgctggcg gtaatcgata agttctttgg
     1561 tgggccggta ggtctggata acctggcggc agccattggc gaagaacgtg aaaccattga
     1621 ggatgtgctg gagccttatc tgattcagca aggctttttg cagcgtacac cgcgtgggcg
     1681 tatggcgacg acgcgggcgt ggaatcactt tggcataacg ccgccagaaa tgccgtaagt
     1741 cggattggtc gctcgcaccg catccgacat tggcagtgtg cctgatgcga cgctattcgc
     1801 gtcttatccg gcctaccaga gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat
     1861 ccgacaaatg tgttcagcga cagattagct ggcctgcttt ttcatcatac tgataataaa
     1921 taacagtgca gcacatagca ctaccgacgg acccgccggc gtatcgtaaa acgcggaaaa
     1981 ggttaaaccg ccagttactg ccaccatccc caccaaaaca gcgacaccag ccatctgttc
     2041 cggcgtacgg gcaaagcgac gcgcagtagc tgcaggaata atcagcaacg aagtaataat
     2101 caacgcaccg acaaatttca tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa
     2161 caatttcacg cgctgtaatt tcacaccatc aacaaacgcc agatccgggc taatcgtcat
     2221 cgacagcaaa ttgcgccatt gccagaacaa aatagccacc acgatgacca cgccaatcgc
     2281 aatagagatg agatcttctg gcgtcactgc cagcaaatca ccgaacaggt aagccatcaa
     2341 atcaacacga atattagaca tcagactaac gaccaccagg cccagcgaca gggcactgtg
     2401 cgccataatc cctaataacg tgtcgatcgc cagctgtgga cgcttctcca gccataccag
     2461 accgcccgcc agcagcagcg taacggcaat caccgcatag aatggattca cgtccagcaa
     2521 caaaccaaac gcgacaccaa gtaatgaggc atgagccagc gtatcaccga aataagacat
     2581 acgacgccag actacaaacg aacccagcgg acccgcggca caggcgagca tgatcccggc
     2641 taaccaaccg ggaaataata attcaatcat gagcgatcat ttccccgacg caaaacaatt
     2701 cgtccctgta aatcgtgacg atggttatga tgatggcgat agatacccag ttgttcagca
     2761 ccacgaggac caaacattga aataaactcc ggatgcaggg aaacaacttc cggtgtgccg
     2821 gaacaacaaa tgtggtgatt caggcacagc acttcatcgg tttttgccat caccagatgc
     2881 agatcgtgag aaaccattaa aacgccacaa tccagttcac gacgcagttg gtcaataagg
     2941 tcatataacg ccacctggcc atttacatcc acgccttgag tgggttcatc cagcactaat
     3001 aattgcgggc gatttaacaa tgctcgcgct aacagtacac gctgcgtttc gccaccagag
     3061 agcttttgca tcggtgcgtt aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa
     3121 atatcttctt tatgtgtacc agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac
     3181 gtggtgtcga gatacagctt ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg
     3241 ataacccctt catcgggtgt taccagcccg agcactaccc gtaccagtgt cgacttacct
     3301 gcgccatttg gcccaagtaa agtcaaaatt tttccaggtt taagttccag cgacacatca
     3361 gagaggacgc ggcgttggcc aaaagaaacc gagacatttt ccaaggaaac cagacttgtc
     3421 atgttaattt tagtcttgca gtagtcatga aatgttataa tatcacactt ctcatattca
     3481 ttacgatgat tggtcgcatt atgttacata aaaaaacgct tcttttcgca gcattatccg
     3541 ccgctctctg gggaggtgca acacaggccg cagatgccgc cgttgtcgct tcgcttaaac
     3601 ccgttgggtt catcgcttct gccattgctg atggggtaac agaaacagag gttttacttc
     3661 ctgacggcgc ttcagaacat gattattcac tgcgcccatc ggatgtaaaa cgcttacaga
     3721 acgcggactt agtcgtttgg gttggcccgg agatggaagc gtttatgcaa aaaccggtaa
     3781 gcaaattacc aggagcgaag caggtaacga ttgcgcagct tgaagatgtg aaaccgctac
     3841 tgatgaaaag tattcacggc gatgatgatg atcacgacca cgcggaaaaa agtgacgaag
     3901 atcaccatca cggcgatttc aacatgcatc tttggctttc cccagagata gcgcgggcta
     3961 cagcggttgc aatccatgga aaattagtgg aacttatgcc gcaaagtcga gccaaacttg
     4021 acgccaacct gaaggatttt gaggcacaat tagcctcaac cgaaacgcag gttggtaacg
     4081 agctcgcgcc gctcaagggg aaaggttatt tcgtttttca cgatgcttac ggctatttcg
     4141 aaaaacagtt cggactgaca ccgcttggtc attttaccgt taaccctgag attcaacctg
     4201 gcgcgcagcg tttacatgaa ataagaacac agttggttga gcaaaaagca acctgcgttt
     4261 ttgctgagcc acagttcagg ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc
     4321 gtatgggaac gttggatccc ctggggacga atatcaaact gggtaaaaca agctattcag
     4381 aattcctgag tcaattagcc aaccagtatg cgagctgcct gaaaggagat taatgaggaa
     4441 gtgattacgt gcaacagata gcccgctctg tcgccctggc gtttaataat ttaccgcgac
     4501 cacaccgcgt tatgttgggg tcgctcaccg ttcttactct ggccgtcgct gtctggcggc
     4561 cctatgttta tcaccgcgat gccacgccaa ttgtcaaaac cattgagctg gaacagaacg
     4621 aaattcgttc gctcttacct gaagccagtg agccgattga tcaagctgca caagaagatg
     4681 aagccattcc ccaggacgaa ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat
     4741 atgttgtttc cactggcgat acgctaagca gcattctcaa tcagtatggt attgatatgg
     4801 gtgatatcac ccaactggct gcggccgaca aagaattgcg taacctgaaa atcggtcaac
     4861 aactctcctg gacattaacc gcggacggcg aactgcagcg cctcacctgg gaagtgtctc
     4921 gtcgtgaaac ccgaacctat gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa
     4981 tgcagcaagg agagtgggtt aacaatctgc tgaaaggtac cgtcgggggg agctttgttg
     5041 ccagcgccag aaacgccggt ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc
     5101 agtggcaaat ggatttccgc aaactgaaaa aaggcgatga atttgcggtg ttaatgtctc
     5161 gagaaatgct tgatggtaaa cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag
     5221 aaggtaaaga ttattacgct atccgcgctg aagatggcaa attctacgat cgtaacggta
     5281 ctggtctggc gaaaggattc ttgcgattcc cgacggcgaa acagttccgt atctcgtcta
     5341 actttaaccc gcgtcgtact aatccggtga ccggtcgcgt tgcagcacac agaggtgttg
     5401 atttcgccat gccgcagggt acgccagtgc tttcagtggg tgacggtgaa gtggtggttg
     5461 ccaaacgcag tggcgcagca ggttattatg tggctattcg tcatggtcgc agctacacca
     5521 cgcgttatat gcacttgcgc aagattctgg tgaaaccggg acagaaggtg aaacgtggcg
     5581 accgtatcgc gctttccggt aataccggac gttcaaccgg gccgcatctg cactatgaag
     5641 tatggataaa ccagcaggcc gtaaacccgc tgacggcaaa actgccgcgt accgaagggc
     5701 tgaccggctc cgatcgtcgc gaattcctgg cgcaggccaa agagattgtg ccgcagctac
     5761 ggtttgatta attaacatcc attcgcagcc ggtacgcagt cagtatcggc tttttttatt
     5821 tggtgcgggg caagttgcgc cgctatacta tcaccagatt gatttttgcc ttatccgaaa
     5881 ctggaaaagc atggaaacga aaaaaaataa tagcgaatac attcctgagt ttgataaatc
     5941 ctttcgccac ccgcgctact ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc
     6001 tttaacgccg ccaaagttcc gtgatcccat tctggcacgg ctgggacgtt ttgccggacg
     6061 actgggaaaa agctcacgcc gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg
     6121 tagtgaagct gaacgcgaag cgattgttga tgagatgttt gccaccgcgc cgcaagcgat
     6181 ggcaatgatg gctgagttgg caatacgcgg accggagaaa attcagccgc gcgttgactg
     6241 gcaagggctg gagatcatcg aagagatgag gcgtaataac gagaaagtta tctttctggt
     6301 gccgcacggt tgggccgtcg atattcctgc catgctgatg gcctcgcaag ggcagaaaat
     6361 ggcagcgatg ttccataatc agggcaaccc ggtttttgat tatgtctgga acacggtgcg
     6421 tcgtcgcttt ggcggtcgtc tgcatgcgag aaatgacggt attaaaccat tcatccagtc
     6481 ggtacgtcag gggtactggg gatattattt acccgatcag gatcatggcc cagagcacag
     6541 cgaatttgtg gatttctttg ccacctataa agcgacgttg cccgcgattg gtcgtttgat
     6601 gaaagtgtgc cgtgcgcgcg ttgttccgct gtttccgatt tatgatggca agacgcaccg
     6661 tctgacgatt caggtgcgcc caccgatgga tgatctgtta gaggcggatg atcatacgat
     6721 tgcgcggcgg atgaatgaag aagtcgagat ttttgttggt ccgcgaccag aacaatacac
     6781 ctggatatta aaattgctga aaactcgcaa accgggcgaa atccaaccgt ataagcgtaa
     6841 agatctttat cctatcaaat aaaaaaagcc tctcaaatta ttgagaggct gtactcgata
     6901 aaaaatctca atgagtgttc ccactatttt gagtttttag gttagtacgc tattgagtta
     6961 atcgtagcgt gataatttca aaaggtttga actcaagatt gcaatattga ttgcaataaa
     7021 aactgtcatc taacttattt tcttgaagat cgacatattg aacgtagtga taattacata
     7081 ctcacgaaag catggctgaa atttctaatc catgactctc atataatctt acgattatac
     7141 cgtcgccgtc ttcagctaat tttattgttt cgatgataac gctgttagta tcgatagcaa
     7201 aaaatgatag tgaagggggt aattcacccg ggtgcggttt gacagggacg acctgtaagg
     7261 gatgattcat tatataggct tgattaataa caccacccac acggtaatct ccatgatgag
     7321 gaaataagga gaatatgaat ttttgtttac caatatctgc aaaattatta atcaggaaat
     7381 tatcataatt gtctgtctta acaacttccc caggtttatg ctgcgaacgc agcaaagtta
     7441 attcaagcgt ctggtctttg acacgatagc catatttgtt ttttgctatt aaagctatgc
     7501 cagtttctcg atcagaaagg tcgacccagt gatgtgctgg tatttcatct ttggcaaaag
     7561 cgaacgaatc atcgctatgc gttggtctgt caattacacc gaactgaatt tgacattgtg
     7621 caagagatgt ttgtactgac accgggaatt ttactttgag agaaacatta ggttcgtgcc
     7681 aatcaataaa cgtttggaca tcaacgcgtg gactatcctg tgtcagggta aaggctaaac
     7741 gaagataaga ggaattgtag tgataaatca gcgtcatcgt agttttaggc ccgctgatca
     7801 cagttgtcat tttttcagcg agtaaaacat atgattcccc atcacgataa tttgaagcaa
     7861 aatcccaggc cgcgaaaaaa cctgcatctt cgtggtatgc acggatctca ctgtgcatat
     7921 tttcagtaat gtattcttta ccatatcttt tatcatatag cgaaattaaa tcaccgcttt
     7981 ctgaaaaaag aagtttaatg taattatttt caatactttg agattctgcc ttcagaccat
     8041 ttacgatctt attctttgga acaatcacag cataaccata agagtttact cgtgctttga
     8101 gccagttgtt gtttatattg atccattcgt tacgagcaaa acaggtggta ttaaacaaaa
     8161 tgtgcggatc tttataagaa gaagtgtcaa ttttacttaa taaagtagac tgcgcatcag
     8221 aaataatttt ctcggttttt gcttctagtt taagatattc cttttcagtt tcatgataaa
     8281 ctcttgaaat acatgagccg ggaaggatgt catggaattg caaggtcagc atcgctttcc
     8341 atatttcatc aaactcactg cgaagaattg atgtcatatt attagtaata gt
//
LOCUS       DAZOXR010000180         8362 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZOXR010000180 DAZOXR010000000
VERSION     DAZOXR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.186"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(113..189)
                     /locus_tag="ACHUNN_003581"
     tRNA            complement(113..189)
                     /locus_tag="ACHUNN_003581"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Asp,seq:gtc)
     gene            complement(322..1053)
                     /gene="dnaQ"
                     /locus_tag="ACHUNN_003582"
     CDS             complement(322..1053)
                     /gene="dnaQ"
                     /locus_tag="ACHUNN_003582"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0577857.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1118..1585
                     /gene="rnhA"
                     /locus_tag="ACHUNN_003583"
     CDS             1118..1585
                     /gene="rnhA"
                     /locus_tag="ACHUNN_003583"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0577858.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1582..2304)
                     /locus_tag="ACHUNN_003584"
     CDS             complement(1582..2304)
                     /locus_tag="ACHUNN_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0577859.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2338..3093
                     /gene="gloB"
                     /locus_tag="ACHUNN_003585"
     CDS             2338..3093
                     /gene="gloB"
                     /locus_tag="ACHUNN_003585"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0577860.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3165..4523
                     /gene="mltD"
                     /locus_tag="ACHUNN_003586"
     CDS             3165..4523
                     /gene="mltD"
                     /locus_tag="ACHUNN_003586"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0577861.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4572..5195)
                     /locus_tag="ACHUNN_003587"
     CDS             complement(4572..5195)
                     /locus_tag="ACHUNN_003587"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0577862.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5199..5999)
                     /locus_tag="ACHUNN_003588"
     CDS             complement(5199..5999)
                     /locus_tag="ACHUNN_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0577863.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6240..7154
                     /gene="yafC"
                     /locus_tag="ACHUNN_003589"
     CDS             6240..7154
                     /gene="yafC"
                     /locus_tag="ACHUNN_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0577864.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7151..7954)
                     /gene="dkgB"
                     /locus_tag="ACHUNN_003590"
     CDS             complement(7151..7954)
                     /gene="dkgB"
                     /locus_tag="ACHUNN_003590"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0577865.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8117..8193)
                     /locus_tag="ACHUNN_003591"
     tRNA            complement(8117..8193)
                     /locus_tag="ACHUNN_003591"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8157..8159),aa:Asp,seq:gtc)
BASE COUNT         2232 a         2015 c         2023 g         2092 t
ORIGIN      
        1 acatttacag cataacaaaa ccatagaccg aagaaatagc gggatttaaa tgcaaaaaaa
       61 ccgcaaaatt aaaaatcttg cggttctctg aactcatttt catgagtgaa tttggcggaa
      121 cggacgggac tcgaacccgc gcccccctgc gtgacaggca ggtattctaa ccgactgaac
      181 taccgctccg cgttgtgttc cgttgggaac gaggcgaata ttacggattg cctcaccctt
      241 cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca agccgctatt
      301 tttagcgcct tttacaggta tttatgcccg ccagaggcag cttccgccct tcttctccac
      361 cagatcaaga cgggcttcat gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa
      421 cttacttgcc tgacgtacaa tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc
      481 tccttccatc gcaaaagcca tcgacgtttg accaccggtc atcgccagat aaacttccgc
      541 aaggatctgt gcatcgagta atgccccgtg tagcgttcgt ttactgttat ctatttcgta
      601 gcgggcacat aacgcatcga ggctgttgcg cttaccagga aacattttcc tcgccaccgc
      661 aaggctatcg gtgaccttac agaaggtatt ggtcttcgga atatcgcgct taagcaacga
      721 aaactcgtag tccataaagc cgatatcgaa cgctgcgtta tggatcacca actccgcgcc
      781 gcggatatag tccatgaact catcggctac ttcggcaaac gtgggcttat cgagcaaaaa
      841 ttcatcggca ataccatgta cgccaaaggc ttccggatcc accagccgat cgggtttgag
      901 ataaacatgg aagttattgc ccgtcaggcg acggttcacc acttcaacgg caccaatctc
      961 aatgatcttg tggccttcat agtgcgcacc aatctggttc ataccggtgg tttcggtatc
     1021 gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc atttatgtca gacttatcgt
     1081 tttacagttc gattcaatta caggaagtct accagagatg cttaaacagg tagaaatttt
     1141 caccgatggt tcgtgtctgg gcaatccagg acctgggggt tacggcgcta ttttacgcta
     1201 tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc accaccaaca accgtatgga
     1261 gttgatggcc gctattgtcg cgctggaggc gttaaaagaa cattgcgaag tcattttgag
     1321 taccgacagc cagtatgtcc gccagggtat cacccagtgg atccataact ggaaaaaacg
     1381 tggctggaaa accgcagaca aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga
     1441 tgctgcccta gggcaacatc aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc
     1501 ggaaaacgaa cgctgtgatg aactggctcg tgctgcggca atgaatccca cactggaaga
     1561 tacgggctac caagtcgaag tttaagcttg tggtttacga cattgccggg tggctccaac
     1621 cgcctggcga attcgtggct tgtttttact ctgtttcatc gggtttagcg ttaaaggaat
     1681 agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa
     1741 tagttttcct ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt gcagcacttc
     1801 aaaattcaac aaagagagcc agtccagttg ccgcatcaga gtaaacatcc ggctgttata
     1861 gggcgaggtt tttcgcaata ccggcacaag tttgcgtaat cccattaaac taatgggatt
     1921 gaagccacta atcaccagcc agccatcatc aatcaatacc cgatcggttt cacgcaataa
     1981 acgatgcgga tcggtgcacc acggcaatgt atgtgccagt agacaaacat caacggattt
     2041 atcggcaaaa ggaagatgaa gtggatccgc ctgcacctgg acgggcattc cttgcgcaga
     2101 aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc
     2161 aatcttaagc agatgaaaac catacatttt agtgaaccac gggttgagct ggcgctccag
     2221 cgccttgcga taaagctctc cccagggcaa atcgccccag cgatcaggag ccacgacagt
     2281 ttgagggact cttgccggtt tcatcacaac cttccgtttc acactaagag gtaatctatg
     2341 aatcttaaca gtattcccgc ctttgatgac aattacatct gggttttgaa tgatgaagca
     2401 ggtcgctgcc tgattgtcga tcccggagac gcagagccag tattaaacgc cattgccgcc
     2461 aataactggc aaccggaggc catatttctc acccaccatc atcacgatca cgttggcggc
     2521 gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt atggtccaca agagacacaa
     2581 gataagggaa caacacaggt agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa
     2641 tttagtgtaa ttgccacacc gggtcacact ttaggacata tctgttactt cagtaaacct
     2701 tatctatttt gcggcgacac gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca
     2761 gcatcacaaa tgtatcaatc acttaataag ttaagtgcgt tacctgacga tacattggta
     2821 tgttgtgctc atgaatatac gttatcaaat atgaagtttg ctttgagtat tcttccgcac
     2881 gatttgtcca taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata
     2941 acactacccg taattctgaa aaatgagcgg caaattaatg tttttttaag aacggaagat
     3001 attgatttaa ttaatgtaat taatgaagaa acattattgc aacaacctga agagcgtttt
     3061 gcatggttaa ggtcaaagaa agataggttc tgataaaact ttcttgtcat cggctccgtt
     3121 cgccgttatg atcggtcgtc ttttaagcaa ctattgacac acacatgaag gcaaaagcga
     3181 tattactcgc ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt caacagcacg
     3241 cacagagcct ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt caggcacgat
     3301 ggatggacga tgggacgtct atcgcgccag atggtgactt gtgggctttc attggcgacg
     3361 agctaaagat gggaattccg gaaaatgacc ggattcgcga acagaaacag aaatatttac
     3421 gcaataagag ctatctccac gatgtaactt tacgggcaga gccgtatatg tactggatag
     3481 cagggcaagt taaaaaacgt aacatgccta tggaactggt actactaccc atagtggaga
     3541 gcgcttttga tcctcacgca acgtctggcg ccaatgccgc aggcatctgg cagatcattc
     3601 cgagcacggg gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg
     3661 ttgttgcttc aacaactgcc gcgctgaata tgatgcagcg tctgaacaaa atgtttgacg
     3721 gcgactggct tctgaccgta gcggcttata acagcggcga aggtcgggtc atgaaggcaa
     3781 ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg gtcgttaccg ttgccgcagg
     3841 aaacgaagca gtacgtgcct aaaatgctgg cattgagtga tattctcaaa aacagcaagc
     3901 gttatggcgt acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga
     3961 gtagcccggt tgaaatggcg aaggttgcag atatggcggg gatttccgtc agcaagctga
     4021 agacattcaa cgctggcgtg aaaggctcca cgctgggcgc aagtgggccg cagtacgtga
     4081 tggtgccaaa gaagcatgca gatcaactgc gtgaatctct cgcttcaggc gaaattgctg
     4141 ctgtacagtc gacgctggtt gccgacaata cgccgcttaa cagccgtgtt tacaccgtac
     4201 gctctggcga cacgctttca agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc
     4261 agcagtggaa caaactgcgc ggatctaagc tgaagccagg ccaaagtttg acgattggtg
     4321 caggcagtag cgcacagcgg ttggcaaaca acagcgatag cattacgtat cgtgtgcgca
     4381 aaggcgattc gctttcaagc attgctaaac gccacggcgt gaacatcaaa gatgtgatgc
     4441 gctggaacag cgacactgcg aatctgcaac caggcgataa gctgacgttg tttgtgaaaa
     4501 acaacaacat gccagattcc tgacaaacca gataataaaa aaggcaccga ttcccccggt
     4561 gcctttttta tttatgccgc tttatgtgct tctaccatga tgatatcact ggtgaaagag
     4621 ccatcagtct gcaaggaaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg
     4681 cgaatagcgt ctactaacgc ttctggcgta cgcattctca cgacccatga agaaaattcc
     4741 agcggtaact tatctgtaat taaattatca actatcagat tggcttcatt gattaacgtc
     4801 aaccacgcac cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc
     4861 tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta catccatcac aatcagccta
     4921 ccgccaggtt tcaatatcct attcacttct cgcagtgctg caccaacatc atgccagtga
     4981 tgggcagaat aacggctgat aacaatatca aatgcgttat cggcaaatgg cagacttgcg
     5041 gcatatccct ggcgggtggt gatatttttc agttgccggg cttcggcagc ttgtgccacg
     5101 acatccagca tgtgggcaga taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg
     5161 ccaaacgttg gtctgccctg cggcagacct gacatacctt atttatcagg cttgccggga
     5221 ctgaattcaa cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttca
     5281 ctgacgttca gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc
     5341 tgatcatcgg taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg atataacgcg
     5401 ttcatccttc tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg
     5461 tgagctatct gatcaccaat aggaagtaac tgcttactat agacatccac gcccagactg
     5521 aagttgacgg cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa tggatagacc
     5581 gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa
     5641 tacactggat gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagcacgaaa
     5701 gcgggtacct gatcggcggc aagatagtta gcggtcgcaa actgtaccaa ctctggcgtt
     5761 gtttgcgctt cctgcaataa caccagatgt gcgtctttgc cgtaattctt taataccgac
     5821 aaccattcag cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg
     5881 gtacttaacg gttctccggg tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt
     5941 aagatcctct ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact
     6001 tttgactttg aaacctctat tacgtcaacc cttccgcact cgggagggtt ctactgttat
     6061 agggatttta gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta
     6121 caccggtcag aaatttgctc attattgaga acattaaatc ggatgacata tcacgcatcc
     6181 tgatggttta atgacaaata aattcaaaat atctcatcta ttttgcaaca ggagcaaaaa
     6241 tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta
     6301 gccgggcagc ggaacaatta gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc
     6361 tggaaatgaa acttggcgtt agcctgctta atcggaccac gcggcaactt agcctgacgg
     6421 aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag
     6481 aatcagaaat tatggagacg cgtaatacac cgcgtggact gttacggatc gatgccgcaa
     6541 ctccagtggt gctgcacttt ctgatgccgt taattaagcc tttccgtgaa cgctatccgg
     6601 aagtcacttt atcgctagtc tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg
     6661 atgtcgcgat acgcgctggt acgttaacgg actccagctt acgtgccagg ccgttattta
     6721 acagttatcg aaaaattatc gcctcccccg attatatttc ccgctacggg aagccagaaa
     6781 cgatcgacga tttaaagcaa catgtttgcc tgggattcac tgaacccgct tccctcaata
     6841 cctggccgat agcctgtagt gatggacaat tacatgaggt gaagtacggt ttgtcatcca
     6901 atagtgggga aacactgaaa cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg
     6961 actacatgat cgacaaagaa atcgctcgcg gagaattggt ggagttaatg gcagataaag
     7021 tgttgccagt ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc
     7081 gcatccgggc ttttatcgat ttccttagcg agcatgtaaa aacagctccc ggaggagctg
     7141 tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggtcgttgc
     7201 aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt tgtgccttaa
     7261 gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca
     7321 tagcccacgc cagaatcact tgtgccggag tcgcattgtg tttagctgca atacgagcaa
     7381 taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg atatgaatac
     7441 cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag agttcaatct
     7501 ggttggtagc gatgttttca gcgccaacag cagcaatcgc tttttccatc aacgggatcg
     7561 tgaagttgga aataccgatc tcacgtgtca gaccttgttt tttggcctcc agcagcgcct
     7621 gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat
     7681 caacataatc ggtacgcaat ttttgcaggc tctcttttag actcgggatc aatttgtctt
     7741 tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac
     7801 tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgca gtatcaattg
     7861 cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg tctttgagac
     7921 ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa tgctattttc
     7981 gttaactgag gtgattatgg gcttcagact gttgcggaaa aagagcataa aatgcagagg
     8041 atttttgcga ttctggcaat aatagatata cagacgaaaa aaaacctcga gcgttaactc
     8101 gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca
     8161 ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa ccaccggttt
     8221 tatgaccggc ttactgctta atttgatgcc tggcagttta tgaatcactt cgggctgttg
     8281 cttcgcaacg ttcaaaagct ttcgcttttg tcctactctc ttatcgagta gtgaactgcc
     8341 tcaaaggtca gtgccattct tc
//
LOCUS       DAZOXR010000181         8217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZOXR010000181 DAZOXR010000000
VERSION     DAZOXR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.187"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUNN_003592"
     CDS             <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUNN_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR0577866.1"
                     /translation="NGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUNN_003593"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUNN_003593"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR0577867.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUNN_003594"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUNN_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0577868.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2031..2549
                     /locus_tag="ACHUNN_003595"
     CDS             2031..2549
                     /locus_tag="ACHUNN_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0577869.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHUNN_003596"
     CDS             complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHUNN_003596"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0577870.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHUNN_003597"
     CDS             4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHUNN_003597"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0577871.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5611..6297
                     /gene="rspR"
                     /locus_tag="ACHUNN_003598"
                     /gene_synonym="ydfH"
     CDS             5611..6297
                     /gene="rspR"
                     /locus_tag="ACHUNN_003598"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0577872.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHUNN_003599"
     CDS             6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHUNN_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0577873.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6713..8173)
                     /locus_tag="ACHUNN_003600"
     CDS             complement(6713..8173)
                     /locus_tag="ACHUNN_003600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0577874.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2173 a         2055 c         1871 g         2118 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc
     1921 ttaaacatgt tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     1981 atacagaaaa agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta
     2041 ataaagataa aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt
     2101 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     2161 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     2221 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg
     2281 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc
     2341 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     2401 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg
     2461 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     2521 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag
     2581 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     2641 ccttaccgcg ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga
     2701 tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact
     2761 ggtaaccatc atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc
     2821 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     2881 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     2941 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     3001 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     3061 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     3121 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     3181 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     3241 ttattacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     3301 agacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     3361 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc
     3421 ccacgccatt atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat
     3481 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     3541 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct
     3601 cccgccgtac ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct
     3661 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg
     3721 ctggaacaat ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg
     3781 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     3841 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     3901 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     3961 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     4021 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     4081 caaccagacc gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg
     4141 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     4201 cggcaaggac aaaacgttga tgaatcacct ccaccaggcg gatggattca ctatcaagcc
     4261 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     4321 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     4381 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     4441 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     4501 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     4561 gatgattggc aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga
     4621 aaggattcat tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta
     4681 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     4741 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     4801 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     4861 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     4921 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     4981 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg
     5041 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     5101 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     5161 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     5221 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg
     5281 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     5341 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     5401 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     5461 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     5521 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     5581 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     5641 cagcctgtca atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     5701 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     5761 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     5821 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt
     5881 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     5941 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     6001 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     6061 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     6121 ttcgaccacg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     6181 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
     6241 agcgaatccg tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc
     6301 atttcctctc atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt
     6361 atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta
     6421 tattgcgccc gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga
     6481 cctacgatcg taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg
     6541 gtcacactgg caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc
     6601 gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc
     6661 gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca
     6721 cggaatattt cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg
     6781 tcacttttgc agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg
     6841 ccagcaatga ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa
     6901 caggtaacag tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat
     6961 aacgcatcca acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat
     7021 gccagcgaac agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct
     7081 gccaggttcg atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat
     7141 cttgcaaatc aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat
     7201 acgccgcata acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa
     7261 gataacccaa atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt
     7321 cataaggcag cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg
     7381 caacaaagtt atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat
     7441 cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa
     7501 tacggtccac cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta
     7561 catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca
     7621 tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg
     7681 cctcaacaat cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga
     7741 ccatcgggtg atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg
     7801 tgattgtcag agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta
     7861 agccatccat ctgtacgtgc aaggctttct taacgacgcc aacgacgcga gccgtccacg
     7921 catcggccga catttccgca acggtataaa gattatcttg ctgttgtaaa tcggcaattt
     7981 gctgttcgcc gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag
     8041 caagaatatc ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa
     8101 taattcttgg ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca
     8161 aattatttcc cataacaatt ccttaaatat aaatatggca aactatatgt tttgtta
//
LOCUS       DAZOXR010000182         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZOXR010000182 DAZOXR010000000
VERSION     DAZOXR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.188"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUNN_003601"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUNN_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR0577875.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUNN_003602"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUNN_003602"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0577876.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUNN_003603"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUNN_003603"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0577877.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHUNN_003604"
     CDS             6679..>8182
                     /locus_tag="ACHUNN_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR0577878.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1978 a         2029 c         2322 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tggcacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZOXR010000183         8157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZOXR010000183 DAZOXR010000000
VERSION     DAZOXR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.189"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..449)
                     /locus_tag="ACHUNN_003605"
     CDS             complement(<1..449)
                     /locus_tag="ACHUNN_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0577879.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHUNN_003606"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHUNN_003606"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0577880.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="ACHUNN_003607"
     CDS             complement(1788..2507)
                     /locus_tag="ACHUNN_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0577881.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="ACHUNN_003608"
     CDS             complement(2691..3017)
                     /locus_tag="ACHUNN_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0577882.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHUNN_003609"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHUNN_003609"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0577883.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="ACHUNN_003610"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="ACHUNN_003610"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0577884.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="ACHUNN_003611"
     CDS             4977..5252
                     /locus_tag="ACHUNN_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0577885.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="ACHUNN_003612"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="ACHUNN_003612"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0577886.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="ACHUNN_003613"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="ACHUNN_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0577887.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="ACHUNN_003614"
     CDS             complement(7903..>8157)
                     /locus_tag="ACHUNN_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0577888.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1923 a         2159 c         1987 g         2088 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaatta
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DAZOXR010000184         8139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZOXR010000184 DAZOXR010000000
VERSION     DAZOXR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.190"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            85..1581
                     /gene="ravA"
                     /locus_tag="ACHUNN_003615"
     CDS             85..1581
                     /gene="ravA"
                     /locus_tag="ACHUNN_003615"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0577889.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUNN_003616"
     CDS             1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUNN_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0577890.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUNN_003617"
     CDS             complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUNN_003617"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0577891.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUNN_003618"
     CDS             4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUNN_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0577892.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUNN_003619"
     CDS             4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUNN_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0577893.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUNN_003620"
     CDS             5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUNN_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0577894.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7498..8121
                     /gene="rsmG"
                     /locus_tag="ACHUNN_003621"
     CDS             7498..8121
                     /gene="rsmG"
                     /locus_tag="ACHUNN_003621"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0577895.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2041 a         2081 c         2083 g         1934 t
ORIGIN      
        1 ctttgagact attttagcca ccagcaagaa acgtctatac tcgcaattta cgcagaactt
       61 ttgacgaaag gacgccactt cattatggct caccctcatt tattagcgga aagaatttcc
      121 cgcctgagca gttcgttgga aaaggggctt tatgaacgta gccacgccat ccgcttgtgt
      181 ttattagcgg cattaagtgg tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa
      241 agtttgatcg cccggcgctt aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg
      301 atgacccgct tctccacgcc ggaagaagtt tttggtcccc tttctattca ggcgctaaaa
      361 gatgaagggc gctatgaacg tttaaccagc ggttacctgc cggaagcaga aattgtcttt
      421 ctggatgaga tctggaaagc gggcccggca attcttaata ccttgctcac cgccattaac
      481 gagcgccagt tccgcaacgg tgcacacgta gaaaaaatcc cgatgcgtct gctggtggcg
      541 gcctccaacg agctgccgga agcagacagc agtctggaag cgttatatga ccgcatgctg
      601 attcgtctgt ggttagataa agtgcaggat aaagcgaatt tccgctccat gctgaccagt
      661 caacaggatg aaaacgacaa tccggttcct gatgccttgc aggtcacaga tgaagaatat
      721 gaacgctggc agaaagagat tggtgaaatt acgctgcccg atcatgtatt tgagctgatt
      781 tttatgctgc gcctgcaact ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc
      841 tggaaaaaag cgatccgatt attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt
      901 gccccggtag atctcatttt gctgaaagat tgcctgtggt atgacgcgca aagcctgaat
      961 ttgatacaac aacaaattga tgtattgatg accggtcatg cctggcaaca gcaagggatg
     1021 ttgactcgcc ttggcgcgat tgtgcaacgt cacctgcaac tccagcagca acaaagcgat
     1081 aaaacagcct taacggtaat tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa
     1141 cttcctgtta acgttactgc ttccactctg actctactgc tgcaaaaacc gttaaaactg
     1201 catgatatgg aagtggttca tatctccttt gagcgtagcg cgctggaaca gtggctgagc
     1261 aaaggtggtg aaattcgcgg taaactgaac ggtatcggct ttgcccagaa actgaatctg
     1321 gaagttgata gcgcccaaca tcttgtcgtt cgcgatgtaa gtttacaagg cagtacgctg
     1381 gcacttcccg gttcatcggc tgaaggtctg cctggtgaaa taaaacaaca actggaagag
     1441 cttgaaagcg actggcgcaa gcaacacgct ttattcagcg aacagcaaaa atgtctgttt
     1501 atccctggcg actggttagg tcgcattgaa gccagcctac aggatgtcgg tgcacagatt
     1561 caccaggcac aacaatgcta acgctggata cgcttaatgt gatgctggcc gtcagcgaag
     1621 agggattgat cgaagagatg atcatcgcac tgctggcctc accgcagctg gcggtcttct
     1681 ttgaaaaatt cccacgcctg aaggcggcaa tcactgatga tgttccccgc tggcgtgagg
     1741 cgctgcgcag tcggctgaaa gatgcccgag tcccgccgga actcaccgaa gaggtgatgt
     1801 gctatcagca aagccagctc ctctccacgc cgcagtttat tgtgcagcta ccacagatcc
     1861 tggacttact gcatcgtctg aattccccat gggcagaaca agcccggcag ttggttgatg
     1921 ctaacagcac aataacttca gcgttacaca cgctttttct ccagcgttgg cgtttaagtc
     1981 tgatcgtgca agcaacgacg ttaaatcaac agctattaga agaagaacgc gaacaactgt
     2041 tgagtgaagt tcaagaacgc atgacgctga gcggacaact tgaaccgatt ctcgcagata
     2101 acaatactgc agctggtcgt ctgtgggata tgagcgccgg tcagcttaaa cgtggcgact
     2161 atcagttgat tgtgaaatac ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg
     2221 cagagcagct ggggcgttct cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg
     2281 aaaccttccg caccatggtg cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc
     2341 aacaaagcga tgatatttta cgtcttctgc cgccagaact ggcgacacta gggataacgg
     2401 aactggagta tgagttttac cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc
     2461 acggtgagtc gtggcgtgaa aaagtgatcg aacgtccggt ggtacataaa gattacgatg
     2521 aacagccgcg cgggccgttt attgtctgtg tggatacttc cggctcaatg ggcggcttta
     2581 atgaacagtg tgcgaaagcg ttctgcctgg ccttgatgcg cattgctctc gctgaaaacc
     2641 ggcgctgcta tattatgcta ttttccaccg agatcgtccg ttatgagctt tcaggccccc
     2701 aaggcatcga acaagcaatc cgttttttaa gccagcagtt tcgtggcggc accgatcttg
     2761 ccagttgttt tcgcgccatt atggaacgct tgcaaagcag ggaatggttt gatgccgatg
     2821 cggtggtgat ttctgatttt atcgctcagc ggttgcctga cgacgtgacg agtaaagtga
     2881 aagagctgca gcgggtacat cagcatcgct ttcatgccgt ggcgatgtcg gcacacggca
     2941 aacccggcat catgcgcatt ttcgatcata tctggcgctt tgataccggg atgcgaagcc
     3001 gcctgctcag acgctggcgg cgataaatta ttacagcaga gaagggacgt tctcgcgaac
     3061 agcagctggc catacaccac actgaacctg gccgatatgc ggcagttgca gcagcagcat
     3121 ggtcaaacga gactggccga taccgccgcc gatggtctgc ggcatttcac cgcgcagcag
     3181 cgcctgatgc cattccagct gcagacgatc ttcatcaccg gtcagcgcca actgatgctt
     3241 caacgtgtcg gcatccacgc ggatccccat ggaagaaagc tcaaacgcat cttccagtac
     3301 agggttccac accagaatat cgccgttcag acccgcatga cccagctctg acggggtgct
     3361 ccagtcatca taatccggtg cgcgcacgtc gtggcgatga ccatcgctca gcttgccgcc
     3421 aatcccgaca aggaataccg cgccaagatc tttcgctatc gcccgctcac gccctttggc
     3481 atcgagatcc ggataacgag acagtaactc ctggctgtgt acgaagtgga tctgatccgg
     3541 caggaacggt gccaggccaa actcttcgct aaccgcagct tcggttgctt taattcccgc
     3601 ccagatcgcc tctaccgtgc ttttcagagt cgagaattga cgctcaccgt cgcccattac
     3661 gcgttcccag tcccactggt caacatagac cgagtgcaac ggagaaagac ggtcttcatc
     3721 ggggcgaagg gctttcatgt gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc
     3781 taaggtctga cgtttccact tcgccagtga atgaaccact tcgaactggg catcaggcag
     3841 agctttcact tttacctgca ccgctttttc acagcccgac aagttatcct gcgtgccatc
     3901 ccccacacgg ctaagaatcg gcgcctggac ttcgatcagc cccagacgtt cttccagttg
     3961 gcgagaaaag tgagatttca cgaagctaat ttggcgttgt ttggcaatgt aagcggtttt
     4021 cattttttat actcctgcgt cctgttgctt atgattaagc aacaaaaacc gacaaacatg
     4081 caataatcat tcaataaaaa gcctaacaca cttttgattc attaaaaaag aaggctaaaa
     4141 tagaatgaat catcaatctg cataagaaaa tcctatggaa aattatctga tcgacaatct
     4201 ggaccgtggc atcctggaag cattaatggg caatgcgcgc accgcttacg ccgaactggc
     4261 gaaacaattt ggcgtcagtc cggggacgat tcacgttcga gtagagaaaa tgaagcaggc
     4321 ggggatcatt accggggcgc gtattgatgt cagcccgaag cagctcggtt atgacgtagg
     4381 ctgctttatc ggcattatat taaagagcgc caaagactac ccttccgcgc tggcaaagct
     4441 ggaaagcctt gctgaagtca ctgaagccta ttacacaaca ggccactaca gcatctttat
     4501 aaaagtaatg tgccgttcga tcgacgctct ccagcatgta cttatcaaca agatccaaac
     4561 aattgatgaa attcagtcca ccgagacatt gatcgtcctg caaaacccga tcatgcgtac
     4621 catcaagcca taatcggctt ttttaatccc atacttttcc acaggtagat cccaacgcgt
     4681 tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga tatcactctt
     4741 atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc tgaaaagctg
     4801 gaagaggcgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga tttatctgcc
     4861 tcggggatct ggctgattat cagctccacc cacggtgccg gagatattcc ggacaacctt
     4921 tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt ccgctttggc
     4981 gcaatcggta ttggtagtcg tgaatatgac accttttgtg gggctatcga taaactcgag
     5041 gcagaactca aaaattccgg tgcaaaacag acaggcgaaa cactgaagat caacattctt
     5101 gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat taatttactc
     5161 aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag cttggatcaa
     5221 ccggtagtta tccaaggaac aacggttgtt cagtttttga gttgtgtata acccctcatt
     5281 ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga tcctttccag
     5341 gttgttgatc ttaaaagccg gatccttgtt atccacaggg tagtgcgatc ctaataagag
     5401 atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca ggatcccagg
     5461 tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat ccattttcat
     5521 accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt catcatcatt
     5581 ggcgggggtc atgcaggcac cgaggccgcg atggccgcgg cgcgtatggg tcaacagact
     5641 ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc ggcgatcggc
     5701 ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa
     5761 gcgatcgatc aagcgggtat ccagtttagg atactaaacg caagcaaagg accggcggtt
     5821 cgcgctaccc gagctcaggc ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg
     5881 gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat tgtcgaaaac
     5941 gatcgcgtgg tcggtgctgt tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg
     6001 ctcaccgttg ggacgttcct cgacggtaaa attcatatcg gtctggataa ttacagcggt
     6061 ggtcgtgctg gtgatccgcc gtccattccg ctttctcgcc gtttgcgtga actgccgctg
     6121 cgcgttggtc gtctgaaaac cgggacacca ccgcgtattg atgctcgaac tatcgacttt
     6181 agtgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt tatgggcaat
     6241 gcgtcccagc atccccagca ggtgccgtgt tatatcactc ataccaacga gaaaacccat
     6301 gatgtgatcc gcagtaacct cgatcgtagc ccaatgtacg caggggtgat cgaaggtgtc
     6361 ggcccacgct actgcccatc gatcgaagac aaagtcatgc gcttcgccga cagaaatcag
     6421 catcagatct tccttgaacc ggaagggctg acctctaacg aaatttatcc gaacggtatc
     6481 tccaccagcc tgccgttcga tgtgcagatg caaatcgtcc gctccatgca ggggatggaa
     6541 aacgcgaaga tcgtgcgtcc gggttatgcc attgagtatg acttcttcga tccacgcgac
     6601 ctgaaaccga cgctggagag caagtttatc caggggctgt tctttgctgg tcagattaac
     6661 ggcactaccg gttacgaaga agccgctgcg caaggtttgc tggtcggtct taacgctgcc
     6721 cgtctgtctg ctgacaaaga aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta
     6781 ctggttgatg acctgtgcac tttaggaacc aaagaaccgt atcgtatgtt tacttcgcgc
     6841 gcagaatatc gtctgatgct gcgcgaagat aatgcggatc tgcgtttgac tgaaatcggt
     6901 cgtgaactgg gcctggtgga tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat
     6961 atcgagcgtg agcgtcagcg tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca
     7021 gccgaagtga atgctcacct gactgcgccg ctttcccgtg aagccagtgg tgaagatctg
     7081 ctgcgtcgtc cggaaatgac ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg
     7141 ttgacagacg aacaggcggc ggaacaggtt gagattcagg ttaaatacga aggttatatc
     7201 gcgcgccagc aagatgagat cgaaaagcag ctgcgtaacg agaacaccct gctacccgcg
     7261 acactggatt accgccaggt atccggtctt tctaacgaag tgatcgccaa acttaacgat
     7321 cacaaaccgg cctctatcgg ccaggcttcg cgtatttctg gcgtcacgcc tgcggctatc
     7381 tccattctgc tggtgtggct gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt
     7441 aaaaatgcct ggtaagcacc cgcttaccag gcaacgcatc aagaacaggt aatcaccgtg
     7501 ctcaacaaac tctccttact gctgaaagac gcaggtattt cgcttaccga tcaccagaaa
     7561 aaccagctta ttgcctacgt gaatatgctg cataaatgga acaaagcgta caacctgact
     7621 tcggtccgcg atcctaatga gatgctggta cgccatattc tcgatagcat tgtggtggca
     7681 ccgtatctgc aaggtgaacg gtttatcgat gtcggcaccg gaccaggact gccaggcatt
     7741 ccactctcta tcgtgcgtcc tgaagcccat ttcactctgt tggatagcct tggtaaacgc
     7801 gtgcgtttcc ttcgtcaggt gcaacatgag cttaaactgg agaatattga gccagtacag
     7861 agcagggtag aagagtttcc ttcagagccg ccatttgatg gcgtaattag ccgcgctttt
     7921 gcctctctga acgatatggt gagctggtgc caccatcttc ctggtgagca aggccgtttc
     7981 tacgcgctga aagggcaaat gccggaagat gaaatcgctt tgttgcccga agaatatcag
     8041 gtcgaatcag tggttaaact tcaggttcca gctctggatg gcgaacgtca tctggtggtg
     8101 attaaagcaa ataaaattta atttttatca aaaaaatca
//
LOCUS       DAZOXR010000185         8127 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZOXR010000185 DAZOXR010000000
VERSION     DAZOXR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.191"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            167..321
                     /locus_tag="ACHUNN_003622"
                     /pseudo
     CDS             167..321
                     /locus_tag="ACHUNN_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            367..783
                     /gene="hicB"
                     /locus_tag="ACHUNN_003623"
     CDS             367..783
                     /gene="hicB"
                     /locus_tag="ACHUNN_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR0577896.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            862..2268
                     /locus_tag="ACHUNN_003624"
     CDS             862..2268
                     /locus_tag="ACHUNN_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFR0577897.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2513..3658
                     /gene="ydcS"
                     /locus_tag="ACHUNN_003625"
     CDS             2513..3658
                     /gene="ydcS"
                     /locus_tag="ACHUNN_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR0577898.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3676..4689
                     /locus_tag="ACHUNN_003626"
     CDS             3676..4689
                     /locus_tag="ACHUNN_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0577899.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4690..5590
                     /locus_tag="ACHUNN_003627"
                     /pseudo
     CDS             4690..5590
                     /locus_tag="ACHUNN_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5580..6374
                     /locus_tag="ACHUNN_003628"
     CDS             5580..6374
                     /locus_tag="ACHUNN_003628"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0577900.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6396..7820
                     /gene="patD"
                     /locus_tag="ACHUNN_003629"
     CDS             6396..7820
                     /gene="patD"
                     /locus_tag="ACHUNN_003629"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0577901.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8030..>8127
                     /locus_tag="ACHUNN_003630"
     CDS             8030..>8127
                     /locus_tag="ACHUNN_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFR0577902.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         1921 a         1908 c         2222 g         2076 t
ORIGIN      
        1 attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc tgattttgca
       61 ttgcattctg ccgttgcggc gatttagtgc tattttcgag caaattacac ataggggtaa
      121 acactaatgt ttactttatt gatatactca gcggcaggga ggcgatgtga aacaaagcga
      181 gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggccgca accatttgaa
      241 actcaggttt catgggaggc gcagtgtcat gccgcgtcac tcctgcgtaa agcaatcctg
      301 aaacaactcg gtttgagtta atcgccaatt aaaaaggtta atgacatgcg agagacagtc
      361 gaaattatgc gttatcccgt cactcttaca cccgcgccgg aaggcggtta tatggtttct
      421 tttgtggata tccctgaagc gttgacgcag ggcgaaactg tcgctgaagc gatggaagcg
      481 gcaaaagatg ctttactgac cgcatttgat ttttattttg aagataacga gcttatcccg
      541 ttaccttcgc cattaaatag tcatgatcac tttattgaag tacctttgag cgtcgcctct
      601 aaagtattgc tgttaaatgc ttttttacag tcagaaatca ctcagcaaga gttagccagg
      661 cgaattggca aacctaaaca ggagattact cgcctattta acttgcacca tgcgacaaaa
      721 atcgacgccg tccagcttgc ggcaaaggcg cttggcaaag agttatcgct ggtgatggtt
      781 taattacagt taacgaaaag ttgtcatttt taacaactga tatagactgc cgaatcatct
      841 gcacataatt acgattcgat aatgaaaaaa taccagcagc ttgcagaaca attacgcgag
      901 cagattgcgt cgggtatctg gcaacccggc gatcgtttgc cttcgttgcg tgaccaggtg
      961 gcgctttcag gcatgagctt tatgactgtc agccatgcct atcagttgct cgaaagtcag
     1021 ggatatatta tcgcacgacc gcagtcgggt tattacgttg cgccacaggc aataaaaatg
     1081 ccgaaagcgc cagtcattcc agtcactcga gatgaagcag tcgatatcaa cacttatatt
     1141 tttgatatgt tgcaggccag tcgcgaaccg tcggtcgttc cgtttgcctc ggcctttccc
     1201 gacccgcgac ttttccccct ccaacaacta aaccgctcgc tggcgcaggt aagcaaaacc
     1261 gccacagcga tgagcgtgat tgaaaactta ccgccaggaa acgcagaact gcgtcaggct
     1321 attgctcgtc gctatgcctt acagggcatc accatttctc ctgatgaaat tgtcattact
     1381 gccggggcgt tagaggcatt aaacctcagt ttgcaagcgg taactgaacc gggcgattgg
     1441 gtgatagtag agaatccttg tttctacggt gcgttgcagg cgctggagcg gctacggctg
     1501 aaggcgttat cggtggcgac ggatgttaaa gaagggatcg atcttcaggc gctggaactg
     1561 gcgttgcagg attatccggt gaaagcgtgc tggctgatga ctaatagcca gaacccactc
     1621 ggatttacct taacgccgca aaaaaaagca caactggtgg cgttactcaa tcagtacaac
     1681 gtaacgctga ttgaagatga cgtttacagc gaactttatt ttggacggga aaaaccgctg
     1741 cctgcgaaag cgtgggatcg ccacgatggc gttttgcatt gctcttcgtt ttcgaaatgt
     1801 ctggtgcctg gttttcgtat tggttgggtc gccgccggaa aacatgcacg taaaattcaa
     1861 cgcttgcagt tgatgagtac gctttccacc agctcaccga tgcaacttgc gctggtggat
     1921 tacctttcca cgcgccgata cgacgcccat cttcgtcgcc tgcgtcgcca gcttgcggaa
     1981 cgtaaacaac gtgcctggca ggcactgctg cgttatctgc ctgcggaagt taaaattcat
     2041 catagtgaca gcggttattt tctctggctg gagctccccg agccgttaga tgccggcgaa
     2101 ttaagcctgg cggcactgac gcatcatatc agtattgcgc cgggtaaaat gttttctacc
     2161 ggtgaaaact ggtcacgttt ttttcgtttt aataccgcgt ggcagtgggg agaacgtgaa
     2221 gaacaggcgg taaaacaatt aggcaaactt attcaagaac ggctgtaata gcgtttaatt
     2281 taattcctct tagattgggt aatatgaatt tcgaatagca gtcatatttc ctaacccctt
     2341 gtctatactc cagaagataa ccttacagac ggcataatgc gcggtagctc acaacctgat
     2401 taaattttct cagggacgaa ggtgtgcctg caagccgccg tctatggtta aacaaggaga
     2461 tatttttacg gcacggcggc tgaacaatta attacgacag gagtaagacc ttatgagcaa
     2521 gacatttgcc cgcagcagcc tgtgtgcgct caccatgaca ataatgaccg ctcacgccgc
     2581 cgaaccacct accaatttag ataaaccgga agggcgactg gatattatcg cctggccggg
     2641 atacatcgaa cgcggacaaa ctgataaaca atacgactgg gtaacgcaat ttgaaaaaga
     2701 gacaggctgc gcggtgaatg tgaaaaccgc cgcgacttcc gatgaaatgg tcagtctgat
     2761 gaccaaaggg ggttacgatc tggttacggc atccggcgat gcctcgctgc gtttgattat
     2821 gggcaaacgc gtgcagccga ttaataccgc cttgattccc aactggaaaa cgctcgatcc
     2881 gcgcgtggtt aaaggcgact ggtttaatgt tggcggcaaa gtttacggca caccttacca
     2941 atgggggccg aacctgctga tgtacaacac taaaaccttc ccgacgccgc cggatagctg
     3001 gcaagtggtt tttgttgagc aaaatctgcc ggacggcaag agcaataaag gccgcgttca
     3061 ggcttatgat ggccctatct acattgcgga cgctgcgttg ttcgttaaag ccactcagcc
     3121 gcagttgggc atcagcgatc cgtatcaact aaccgaagaa cagtaccagg cggtgctgaa
     3181 agtgctgcgc gctcaacaca gtttgatcca tcgctactgg catgacacta ccgtgcaaat
     3241 gagcgatttc aaaaacgagg gtgtggttgc ttccagtgcc tggccctatc aggccaacgc
     3301 cctgaaagcc gaaggccagc ctgttgctac cgttttcccg aaggagggtg ttaccggttg
     3361 ggctgatacc accatgctgc atagcgaagc gaaacatccg gtttgcgcct acaaatggat
     3421 gaactggtca ttaacgccaa aagtgcaggg cgatgtggcg gcctggtttg gctcgttacc
     3481 ggtagtgccg gaagggtgta aagccagtcc gttattaggc gaaaaaggtt gtgaaaccaa
     3541 cggttttaac tatttcgaca aaatcgcctt ctggaaaacg cctatagcag aagggggcaa
     3601 gtttgttccc tacagtcgct ggacgcagga ttacattgcc attatgggcg gtcgctaact
     3661 tcgctggggt gctttatgac gtacgcagtg gagtttgaca acgtctcgcg gttgtacggt
     3721 gacgtgcgcg cagtagatgg cgtcagtatt gcgataaaag atggtgagtt cttctctatg
     3781 ctggggccgt ccggctccgg caaaaccacc tgcctgcgcc tgattgctgg cttcgaacag
     3841 ctttccggcg gggctatctc tatctttggt aaacccgcca gcaatctgcc accgtgggag
     3901 cgggacgtga atactgtctt tcaggactac gcgctatttc cgcatatgtc gattcttgac
     3961 aatgtcgcct atgggctgat ggtcaaaggc gtgaataaaa agcagcggca cgcaatggcg
     4021 caagaggcgc tggagaaagt ggcgttgggg tttgtacagc aacgtaaacc gtcacaactt
     4081 tctggtggtc agcgccagcg ggttgctatc gccagagcat tggtgaatga accgcgcgta
     4141 ttgctgttgg atgaaccgct cggcgcactg gatctcaaat tgcgtgagca gatgcagctg
     4201 gaactgaaaa aactgcaaca gtctctcggt atcactttta tcttcgttac ccacgatcag
     4261 ggcgaagcgt tatcgatgtc cgatcgtgtg gcggttttca ataatggacg cattgagcag
     4321 gtcgattccc cgcgcgatct ctatatgcgc ccgcgcacgc cgtttgttgc cgggttcgtt
     4381 ggtacatcga atgtttttga tggactgatg gcagagaaac tttgtggcat gacgggaagc
     4441 ttcgccctgc gaccggaaca tatccgcctc aacacccctg gtgaactgca ggccaatggc
     4501 acgatccagg cggtgcaata tcagggcgcg gcaactcgtt ttgaactgaa attgaacggc
     4561 ggtgaaaaac tgcttgtgag tcaggccaat atgacaggcg aagaactgcc tgccacgctc
     4621 acgcccggac aacaggtgat ggtttcctgg tcgcgtgatg tgatggtgcc gctggttgag
     4681 gagaggtgaa tggcgatgaa tgtattgcaa tcaccttcac gtccaggtct ggggctgttt
     4741 ttactgctgc ttggcccgct aatgtggttt ggcattgtct atttcggctc gctgctgaca
     4801 ctgttatggc agggatttta tacttttgac gatttcacca tgtcggtaac gccggaactg
     4861 acgctggcga atatccgtgc gctgtttaat ccggcgaatt acgacatcat tctccgcacg
     4921 ctgactatgg ctgtggcagt cactatcgcc agcgccattc tggcttttcc aatggcgtgg
     4981 tatatggcgc gctataccag cgggaaaatg aaagcgtttt tatattgcgg taatgttgcc
     5041 gatgtgggcg agctacattg ttaaagccta tgcctggacg ttattgctgg caaaagatgg
     5101 cgtggctcag tggtttttac aacatcttgg gctggaacca ctgctgactg cgttccttac
     5161 attacctgcg gtgggcggaa atacgctgtc aacttccggg ctggggcgct ttctggtgtt
     5221 tctctatatc tggttgccgt tcatgatcct gcccgttcag gcggcgcttg agcgtttgcc
     5281 gccgtcattg ttgcaggcgt cggctgatct cggcgcacgt ccacgacaaa cctttcgcta
     5341 tgtggtgctg ccgctggcaa tcccgggtat tgccgctggc tctatcttta ccttctcact
     5401 cactctgggc gattttatcg tcccgcagct ggttggtcct ccaggatatt ttatcggcaa
     5461 tatggtttat tcccagcagg gggcgattgg caatatgccg atggcggcgg cattcacctt
     5521 ggtgccgatt attctcatcg cactgtacct ggcgttcgtg aaacgtctgg gagcgttcga
     5581 tgcactctga acgcgcaccg tttttcctca aactggcggc ctggggcggc gttgttttcc
     5641 tacattttcc catcctgata atcgccgcct atgcgtttaa cactgaagat gcggcgttta
     5701 gttttccacc gcagggcctg acgctgcgct ggtttagcgt ggcagcacag cgtagtgata
     5761 ttcttgatgc cgtgacactg tcacttaaag tggcggcgct ggcgacatta attgcgctgg
     5821 tgttagggac gctggcagct gccgcgctgt ggcgacgaga ctttttcggc aaaaacgcca
     5881 tttcgctgtt actgctgctg cccattgcgc tgccgggcag cgtcactggt ctggcgttat
     5941 taaccgcctt taaaaccatc aatctggagc cgggattttt caccatcgtg gtcggtcatg
     6001 cgactttttg tgtagttgtg gtgtttaaca atgtcatcgc ccgttttcgc cgcacctcct
     6061 ggagtctggt tgaggcgtca atggatcttg gggccaatgg ctggcaaacc ttccgctacg
     6121 tagtgttgcc gaatctcagt tcggcgttac tggcaggagg aatgctggcg tttgccttgt
     6181 cgttcgatga aatcatcgtt acgaccttta cggcaggtca tgaacgaacg ttactgttgt
     6241 ggttgctcaa tcagcttggg cgaccgcgtg atgtaccggt aactaacgtg gtggcactgc
     6301 tggttatgtt ggtaacaacc ttgccgatcc tgggggcctg gtggctaacc cgcgaaggcg
     6361 acaatggtca ataaccactg atacaggaat atgctatgca acataagtta ctgattaacg
     6421 gagaactggt tagcggcgaa ggggaaaaac agcctgtcta taatccggca acgggggacg
     6481 ttttactgga aattgccgag gcatccgcag agcaggtcga tgctgctgtg cgcgcggcag
     6541 atgcagcatt tgccgaatgg gggcaaacca cgccgaaagt gcgtgcggaa tgtctgctga
     6601 aactggctga tgttatcgaa gaaaatggtc aggtttttgc cgaactggag tcccgtaatt
     6661 gtggcaaacc gctgcatagt gcgttcaatg atgaaatccc ggcgattgtc gatgtttttc
     6721 gctttttcgc gggtgcggcg cgctgtctga atggtctggc ggcaggtgaa tatcttgaag
     6781 gtcatacttc gatgatccgt cgcgatccgt tgggggtcgt ggcttctatc gcaccgtgga
     6841 attatccgct gatgatggcc gcgtggaaac ttgctccggc gctggcggca gggaactgcg
     6901 tagtgcttaa accatcagaa attaccccgc tgaccgcgtt gaagttggca gagctggcga
     6961 aagatatctt cccggcaggc gtgattaaca tactgtttgg cagaggcaaa acggtgggtg
     7021 atccgctgac cggtcatccc aaagtgcgga tggtgtcgct gacgggctct atcgccaccg
     7081 gcgagcacat catcagccat gccgcgtcgt ccattaagcg tactcatatg gaacttggtg
     7141 gcaaagcgcc agtgattgtt tttgatgatg cggatattga agcagtggtc gaaggtgtac
     7201 gtacatttgg ctattacaat gctggacagg attgtactgc ggcttgtcgg atctacgcgc
     7261 aaaaaggcat ttacgatacg ctggtggaaa aactgggtgc tgcggtggca acgttaaaat
     7321 ctggtgcgcc ggatgacgag tctacggagc ttggaccttt aagctcgctg gcgcatctcg
     7381 aacgcgtcag caaggcagta gaagaggcga aagcgacagg gcacatcaaa gtgatcactg
     7441 gcggtgaaaa gcgcaagggt aatggctatt actatgcgcc gacgctgctg gctggcgcat
     7501 tacaggacga tgccatcgtg caaaaagagg tatttggtcc agtagtgagt gttacgccct
     7561 tcgacaacga agaacaggtg gtgaactggg cgaatgacag ccagtacgga cttgcatctt
     7621 cggtatggac gaaagatgtg ggcagggcgc atcgcgtcag cgcacggctg caatatggtt
     7681 gtacctgggt caatacccat ttcatgctgg taagtgaaat gccgcacggt gggcagaaac
     7741 tttctggtta cggcaaggat atgtcacttt atgggctgga ggattacacc gtcgtccgcc
     7801 acgtcatggt taaacattaa gttagatgtt agtggctgtt ataggacggc cactatcgtt
     7861 ttaggttctg agtttaatgg cggttaagta ttagaatgat tacattaata actagcgtaa
     7921 aaattaggct aaatgtttga tggcgatttt tcatcctgtc acagttgatt agcataaggc
     7981 tttagagtat cgttattaaa ctaataattt ataagtagaa gtattaacaa tgaaagttca
     8041 attgtacaaa ttcacggaag ataaaaataa aacccttacc ttcagatgga cgaaaaaaca
     8101 ctttgagttt tgtatggata ataaaat
//
LOCUS       DAZOXR010000186         8104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZOXR010000186 DAZOXR010000000
VERSION     DAZOXR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.192"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(101..796)
                     /locus_tag="ACHUNN_003631"
     CDS             complement(101..796)
                     /locus_tag="ACHUNN_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0577903.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="ACHUNN_003632"
     CDS             complement(789..2216)
                     /locus_tag="ACHUNN_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0577904.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="ACHUNN_003633"
     CDS             complement(2227..2946)
                     /locus_tag="ACHUNN_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0577905.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="ACHUNN_003634"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="ACHUNN_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR0577906.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /gene="rclA"
                     /locus_tag="ACHUNN_003635"
     CDS             4554..5879
                     /gene="rclA"
                     /locus_tag="ACHUNN_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR0577907.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /gene="rclB"
                     /locus_tag="ACHUNN_003636"
     CDS             5988..6224
                     /gene="rclB"
                     /locus_tag="ACHUNN_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR0577908.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="ACHUNN_003637"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="ACHUNN_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR0577909.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="ACHUNN_003638"
     CDS             complement(6989..7858)
                     /locus_tag="ACHUNN_003638"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0577910.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1732 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAZOXR010000187         8054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZOXR010000187 DAZOXR010000000
VERSION     DAZOXR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.193"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            103..468
                     /locus_tag="ACHUNN_003639"
                     /pseudo
     CDS             103..468
                     /locus_tag="ACHUNN_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
     gene            complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="ACHUNN_003640"
     CDS             complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="ACHUNN_003640"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0577911.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3236..4069
                     /gene="ppsR"
                     /locus_tag="ACHUNN_003641"
     CDS             3236..4069
                     /gene="ppsR"
                     /locus_tag="ACHUNN_003641"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0577912.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4226..5272
                     /gene="aroH"
                     /locus_tag="ACHUNN_003642"
     CDS             4226..5272
                     /gene="aroH"
                     /locus_tag="ACHUNN_003642"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0577913.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5404..5595
                     /gene="hemP"
                     /locus_tag="ACHUNN_003643"
     CDS             5404..5595
                     /gene="hemP"
                     /locus_tag="ACHUNN_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0577914.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5599..7035)
                     /gene="selO"
                     /locus_tag="ACHUNN_003644"
     CDS             complement(5599..7035)
                     /gene="selO"
                     /locus_tag="ACHUNN_003644"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0577915.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="ACHUNN_003645"
     CDS             complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="ACHUNN_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0577916.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2073 a         2113 c         1910 g         1958 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccaaaattac cggacgcaaa
      121 aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtaga agatatttta
      181 ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga acgtttaggt
      241 gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc gctggaagag
      301 gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga acatatcgtg
      361 gtaatcgaaa aactaccgcg tactgcctca ggtaaaatac aaaagttttt gttaagaaaa
      421 gatattatgc ggcggttaac tcaggatgtc tgtgaagaga ttgaataagt ttcatcttcg
      481 gggatcacat aaccccggcg actgaacgcc gccggggatt tattttattt cttcagttca
      541 gccaagctta accaggtttg caccacggtg tccgggttca gagacaggct atcgatcccc
      601 tcttccatca accatgcggc aaagtcttcg tggtcggacg gaccctgacc gcaaatcccg
      661 acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcagtgc tttcaccgca
      721 tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacggtccag accgagcgcc
      781 agctgcgtca tatcgtttga gccaattgag aagccgtcga aatattcgag gaactgctcg
      841 gccagcaagg cgttggacgg gatttcacac atcatgatga ttttcagccc gttctcacca
      901 cgcttcagcc cctgacgcgc cagttcctca accaccgctt tcgcctgatc tacggtacgc
      961 acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac acgtttcact
     1021 gcttcgcact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg tcccgcgcca
     1081 cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc gaccaggttg
     1141 gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg ataaaacgcg
     1201 gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg cggagaatca
     1261 aaacctttca tcatctcgcg gatttcattt tgcaactgcg gttcctgatc gtcaaactca
     1321 agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag acgcgcaagg
     1381 cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt accgacgttc
     1441 atcatcactt tcaacggcag atccggcatc atttctacgc tggagctttt cacgctaaat
     1501 tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac agtgacgttc
     1561 tcaccgtctt tcatccgttc tgttgcatca ccacagccca ctaccgccgg aatgcccagt
     1621 tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac gatggcagat
     1681 gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtgaccag cacgtcacca
     1741 ggttcgatgc ggttcatttc gctgatctca tggatgactt tcaccggacc cgcgccgatg
     1801 cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag cgtataacgc
     1861 tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat gaacagtttg
     1921 ccagtgtggc catctttcgc ccactcaata tccatcgggc gaccgtagtg tttctcaatt
     1981 tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa gatgtcacgc
     2041 tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt cggcgcgtaa
     2101 accatgcgga tttttttcga ccccatggtg cgacgcacga tagccgggcg attcgccgcc
     2161 agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac gaccatttcc
     2221 ccaaggcccc atgcggaagt gataaacacc acctggtcaa agccggattc ggtatcaatg
     2281 gagaacatca cgccagaaga tgcgaggtcg gagcgcacca tccgctgaac accggcagag
     2341 agcgccacgc cacggtgatc gtaaccctgg tgcacacgat aagagatggc gcgatcgtta
     2401 aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc ctgaacgttg
     2461 aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc ggtggcggag
     2521 gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata ggcttcgcgg
     2581 atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg gatttgcgcg
     2641 cccgctttcg caagctgagt aacatcgtca atatccgttt tatccagcag ttcataaatg
     2701 cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt tgtggcgaaa
     2761 ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc cagggaggca
     2821 tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca aagcaccagc
     2881 ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac ggtttgagaa
     2941 acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg gtgaatcgtt
     3001 caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa atctgctctc
     3061 ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaaatga aatgctgttt
     3121 tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgtcc tacgcttctg
     3181 tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga acaaaatgga
     3241 taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa ctgcggaggt
     3301 attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta cgctgccgtt
     3361 tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa tttatcacca
     3421 gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc gcgccatcat
     3481 cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc cgctacaaca
     3541 agagatgaaa ctggatccaa cgccgattgc tcatcgtacc catggcctta accctaataa
     3601 tctcaataaa tatgatgcgc gcattgcggc gattgattac accctcgccc acgatgacgg
     3661 catttcgttg cgcaatctgg atcaggctca ggtgatcctg ctcggtgttt ctcgctgtgg
     3721 taaaaccccc accagtcttt atctggcaat gcaatttggt atccgcgcgg caaactaccc
     3781 ctttattgcc gacgatatgg ataatctggt gctacccgcg tcgctcaaac cgcttcagca
     3841 taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg aggaacgtcg
     3901 ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg aagtggaagc
     3961 gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg tagaagagat
     4021 tgccaccaag atcctcgata tcatgggcct tagtcgccga atgtactaga gaactagtgc
     4081 attagcttat ttttttgtta tgatgctaac cacccggcga ggtgtgacac acctcgcact
     4141 tgaaatcagc agcgattggt ttatcgtgat gcgcatcact tcccggcagt cctgccgtag
     4201 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
     4261 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgccgc
     4321 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctcaatggtg aagataagcg
     4381 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     4441 cccccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     4501 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaagga ctaatctccg atccagattt
     4561 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     4621 aaatgagctg ggcgtcccaa ccgcgactga gttcctcgat atggtgaccg gtcagtttat
     4681 tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc agatccaccg
     4741 tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg atggcaatac
     4801 gcggattgct gtggatgcta tccgcgcagc ccgcgccagc catatgttcc tctcgccaga
     4861 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     4921 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     4981 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     5041 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     5101 tacggcgatt gctggaatta tggcggaaag tttcctgctc gaaggaacgc aaaaaatcgt
     5161 cggcagtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     5221 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     5281 ccattcctga cggaatgggc atttctgcgc aacttgttgt cttctcaaca aattactgct
     5341 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatc
     5401 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggacgccaa tcacaaggcc
     5461 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     5521 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     5581 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     5641 tggacgactg acatagtcat catctctatc gctgaaagga tttcgcaacg ccccatgcag
     5701 gcggtgcaat tccgtcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     5761 gttgcgcaat accagagcgg ggttaacgct ttgcatcaac tgctgacgct cactatcgct
     5821 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagccatcaa atgccgcacg
     5881 atcaataaac tcatcacgta gcggtgacgc cgtgctgtgc tgctcggtca gactcagcat
     5941 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     6001 tagcgcgtta tcctcttttt gctccgtgat gaagcccagt ttctgccgca tccgttgtcc
     6061 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     6121 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     6181 attatcaaaa ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     6241 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     6301 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     6361 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     6421 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     6481 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     6541 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     6601 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     6661 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     6721 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     6781 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     6841 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     6901 cgatggaata ctcagcgtgt tagccagttc ggtattatgc caaatcagcc gggcattatt
     6961 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     7021 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     7081 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     7141 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     7201 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctggatc
     7261 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     7321 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     7381 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     7441 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     7501 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     7561 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     7621 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     7681 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     7741 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     7801 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     7861 tgaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     7921 ctttttcagg ggtccttttt ttagattatc ctgattataa acgaataatc catttacgga
     7981 atttttgtct gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc
     8041 gtccattttt cgac
//
LOCUS       DAZOXR010000188         8052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZOXR010000188 DAZOXR010000000
VERSION     DAZOXR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.194"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            397..1083
                     /locus_tag="ACHUNN_003646"
                     /pseudo
     CDS             397..1083
                     /locus_tag="ACHUNN_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1416..2291
                     /locus_tag="ACHUNN_003647"
     CDS             1416..2291
                     /locus_tag="ACHUNN_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR0577917.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2299..3075
                     /locus_tag="ACHUNN_003648"
     CDS             2299..3075
                     /locus_tag="ACHUNN_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR0577918.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            3244..5505
                     /locus_tag="ACHUNN_003649"
     CDS             3244..5505
                     /locus_tag="ACHUNN_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0577919.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYHSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            5574..6749
                     /gene="senB"
                     /locus_tag="ACHUNN_003650"
     CDS             5574..6749
                     /gene="senB"
                     /locus_tag="ACHUNN_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR0577920.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(7275..7736)
                     /locus_tag="ACHUNN_003651"
                     /pseudo
     CDS             complement(7275..7736)
                     /locus_tag="ACHUNN_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(7774..>8052)
                     /locus_tag="ACHUNN_003652"
     CDS             complement(7774..>8052)
                     /locus_tag="ACHUNN_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR0577921.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         2221 a         1890 c         1901 g         2040 t
ORIGIN      
        1 ttcgcaaaaa ctggtcgcag aaaagtcctg ttttcagctt ggaaacccgg taatcagaat
       61 aatggatagg ggaacaaaaa tctctgtata gaacgctcca ttgagggaga gaaaacgtgt
      121 cattttgacc cgttcggtat tcagaacaca caaaaccgat cgcgggtcgc tttcgtgcat
      181 agttatgcaa cgcgctaaaa acgattctga cgcatttttt atgtttaccc tgttttttct
      241 tgtctttttg agttttttgc gtcagagagc gtctgagagg gcacgtacaa cgggagttat
      301 ggtaaatgga tcgggttttc gggaaggttc gacaggattt gccgttgggt gtagtgtaag
      361 cgactgaaaa acaaacgccc ctgaaatcat gtccagatcc ggcagaacca gcctcatgcg
      421 cagttctggc tggtttttga cgttgatcgc gagggggcag cgattgactg gagcgaccgg
      481 aacgctcccg cgccgaacat caccgtaaaa aatcctgtga acggtcatgc tcacctgctc
      541 tacgcgctca atatcgccgt gagaaccgcg ccggacagct cggttaaggc gctgaaatac
      601 gccgctgcga ttgaacgttc gctgtgtgag aaactttgtg ctgatgtgaa ctacagcgga
      661 ctgatttgta aaaatccgtt ccacctggaa tggcaggtga tggagtggcg agaggaagcc
      721 tataccctcg atgaactggc tgattatctc gatttgagcg cctcagcgcg ccgtagcatc
      781 gataaacatt acgggatggg gcgaaactgc cacctgttcg aaatgacgcg caaatgggct
      841 tacagggcga ttcgtcaggg ctggcccgaa ttctcacagt ggcttgatgc cgtgattcag
      901 cgtgtcgaaa tgtacaacgc atcgcttccc gttccgcttt caccgcctga atgtcgggct
      961 attggcaaga gcattgcgaa atacacgcac aggaacttca cgccggaaac tttcgcacag
     1021 tatgtggctg atacgcacac attaacgtac gttttcgttc cattggccct caccccccga
     1081 taacgaccca ggtagcgcgc cgtattcccc ccgccggccg ttttcttcga catgtacatc
     1141 aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg atagattcaa
     1201 gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat agatccgttt
     1261 tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat tctttttgtt
     1321 ggtaatgata atcatttaca ttcattcttt gatgttttat cataattaat gttttgtcaa
     1381 taaatatatg gaaacatatt gtttcagatg ggaatatgag aaagttcatc cttatttcta
     1441 tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc ataaagcagg
     1501 atgcgccagg gcagaaagct ttcctgactg acgggcagat ttacgatttg cacagtggca
     1561 aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat cttattattg
     1621 gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt caaaatttgc
     1681 tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagag caacagcccc
     1741 gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat agccgtgttc
     1801 aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac atcgtcatgc
     1861 agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag cagatgttgg
     1921 cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct gtggtacaag
     1981 aagctctgcg tgaaaccatc atatctatgc atgaaggcaa ccttgaaagc cagcaactga
     2041 cgtcaatgct ctctattcag cagcagcggg atcgctacat ggcccgccag cttctgagtg
     2101 ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaagc atgggcgtac
     2161 cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg cttgcagaaa
     2221 aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg ccagatacaa
     2281 caaaaaggta aaccatggat gatgaatatt ctccacttct cacagtcggt aaaatggagc
     2341 agctggttta tctgttctct actgcttcat ggactgattt tccttgcgtt tatctggcgt
     2401 ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca atgggcagaa
     2461 attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca acaggagtcc
     2521 gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt cactgaagac
     2581 agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga aaagaaaaag
     2641 acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac agccgtttcc
     2701 agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc ttttaacagt
     2761 gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa aggccaccta
     2821 aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg cacagccgtc
     2881 gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga aatctcttca
     2941 ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca accacttccc
     3001 aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc gattaccttt
     3061 aagttgaagg agtaaaggga taattggccg ttcaagttca tttaactgaa atttaatttt
     3121 catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca aaaagacagt
     3181 agaacataat taataaacaa atagttatac ttatctttat taatattata cccaaggaaa
     3241 tttatgaacg ttataaaact ggctatcggc tcaggcatat tattgctcag ctgcggtgct
     3301 tactcacaat ccatcagtga aaaaactaat tccgacaaaa aaggagcggc agaattcagt
     3361 ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg aagctctcga gaaaacaggc
     3421 gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac agtgcgtagt
     3481 atgcctggta cttatactca aatagatcct ggtcagggag caatcagtgt gaatattcga
     3541 ggcatgagcg gatttggtcg tgtaaacact atggtcgatg gtattaccca gagtttttac
     3601 ggaacctcta cctccggaac aacgacgcat ggttcaacta acaatatggc tggcgtactt
     3661 atagatccta acttactggt agcagttgat gttacacgcg gtgacagcag tggctctgaa
     3721 gggatcaacg cccttgccgg tagtgcaaat atgcgtacta ttggcgttga cgatgtaata
     3781 tttaacggta atacatatgg ccttcgttca cgtttctctg tcggtagtaa tgggctggga
     3841 cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt ttacggatac gggaagcatt
     3901 ggcgttatgg ctgctgtgag cggcagttct gtgtactcta atttctcaaa tggttctgga
     3961 attaacagca aagagtttgg ttatgataaa tatatgaagc agaaccccaa atcccaactg
     4021 tataaaatgg atatcagacc agacgaattt aacagcttcg aactttccgc tcgaacctat
     4081 gaaaataaat ttacacgtcg tgatataacc agtgacgact attacattaa atatcattac
     4141 accccttttt ctgaattaat tgactttaac gtaacggcca gtaccagtcg cggtaatcaa
     4201 aagtatcgtg atggctcgct gtatactttc tacaaaacct cagcgcaaaa tcgttctgac
     4261 gcgctggata tcaacaatac cagccggttc actgtcgcgg acaatgacct ggagtttatg
     4321 ctgggcagca aactgatgcg tacccgctat gaccggacca ttcactcagc ggcgggcgac
     4381 ccgaaagcga atcaggaatc gatcgagaac aatccgttcg caccctccgg ccagcaggat
     4441 atttcagcgc tgtataccgg gctgaaggtt acgcgcggca tctgggaggc agatttcaat
     4501 ctcaactaca cacgtaacag gatcacaggg tacaagcccg cctgcgattc acgcgttatc
     4561 tgcgtgccac agggtagcta cgatattgac gataaagagg gtggcttcaa cccttcagtt
     4621 cagctttctg ctcaggtaac accatggctt cagccgttca ttggctacag caaatccatg
     4681 cgcgccccga acatccagga gatgttcttc tctaattcag gaggcgcatc catgaaccca
     4741 ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt ttaacattga taccagagat
     4801 ttactggtcg aacaggatgc cctgcgcttt aaggctctgg cgtaccacag caggatccag
     4861 aactacatct acagcgagtc ttatctggtt tgttctggag gtcgtaaatg cagtatgcct
     4921 gaggtgattg gcaatgactg ggagggcatt agcgatgaat acagcgacaa tatgtacatc
     4981 tacgttaact cggcaagcga cgttatcgca aagggcttcg aactggagat ggattatgat
     5041 gcaggttttg cttttggccg actctctttc agccagcagc aaacagacca gccaacctcc
     5101 atcgccagca cctactttgg cgcaggggat atgaccgaac tgcccagaaa atacatgacg
     5161 ctggatactg gtgttcgctt cttcgataac gcgttgaccc tgggcactat cataaaatac
     5221 acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac cggcgcaata
     5281 atcaaacagg atttgccgca gatcccaacg attatcgatc tctatggtac ttacgagtac
     5341 aaccgcaacc taacactgaa actttcggta caaaacctga tgaacagaga ttattcggag
     5401 gcgctgaata agctcaacat gatgccaggt cttggtgacg agacccaccc agccaattcc
     5461 gcgcgtggca gaacatggat atttggcggg gacattcgtt tctgatgaca atttccgcta
     5521 ggttcagaca atacgttttc tctcttatgt caattttatt gcaggaacga aaaatgaata
     5581 ttttcacttt atccaaagca ccgctatacc tgttaatttc actattttta cccacgatgg
     5641 ccatggctat cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa acgaattacc
     5701 ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga taatgccagt aaaaaggtgt
     5761 ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa aggctatctg tattcgttta
     5821 attcagattc gctgaaagtc gaaaataaat acacgatgcc ataccgggca ttttcgctgg
     5881 cgataaatca ggataaacat cagctctata tcggacacac ccagtcagcg tccctgcgta
     5941 tcagtatgtt tgacacccca accggcaaac tggtaagaac cagcgacagg ttaagtttta
     6001 aagcggcaaa cgctgcagat tcgcgttttg agcattttcg ccatatggtt tacagccagg
     6061 attccgatac cctgtttgtg agttatagca atatgctgaa aacggccgag ggcatgaagc
     6121 ctctgcataa gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc gaggttaagg
     6181 atgcttacaa aggtacagcg tatggtctga cgatggatga aaaaacacag aaaatctacg
     6241 ttggcggaag agattacatc aacgaaattg atgcgaaaaa tcagacgctg ctgcgtacca
     6301 tcccgttgaa agatccgaga ccacaaatca caagtgtgca gaatctggcg gtggactccg
     6361 cttctgaccg tgcctttgtg gtggtattcg accatgacga tcgttccggt acaaaagatg
     6421 gactctatat ttttgactta cgcgacggta aacagcttgg ctatgtgcac acaggagccg
     6481 gagctaacgc ggtgaaatac aatccgaaat ataacgaact gtatgtcacc aacttcacta
     6541 gcggcaccat cagcgtagtg gatgccacca aatacagcat cacccgtgaa tttaacatgc
     6601 cggtctaccc aaaccagatg gtgttgtcgg acgatatgga taccctttac attggcatca
     6661 aagaaggctt taaccgcgat tgggatcctg atgtgtttgt ggaaggagct aaagaacgta
     6721 ttctgagcat tgatttgaaa aagtcgtgat cggggggaaa aaacatggga taaaaactcc
     6781 gttattccac taaactggcc ggactaggaa aagcaaacat ctctgtcctg ccggtacgcc
     6841 tgttacgccg ctccatgagg ctgaggaatc tccagaaaac gagacaggca cagcattgta
     6901 cgatcaactg atttgtgcca ataattaaat gaaatcacag ctatgcctgt tcatggggtt
     6961 ctaggccgga accccagatt tttccgattg tttgttttct aactgattat tcagtagttt
     7021 tagagggctg acgctcagtg gaacgaaaac tcacgttaag aggttttgac tatgataaaa
     7081 gtcaaagtag gggtgagttt ggatatggaa attattgtac tataaggaga gcatgaaaac
     7141 ccctggggca gtcactgaat gagggaccag cagcggtaaa ataaccagca taatttctgc
     7201 atggtcgatt tttataccgt gacaggctgt tttttatact tggggtcagt ttggatatcg
     7261 aaaattattg tacgttaagg ttgttttttg accttgtcga tcttaactga tctcaaacga
     7321 cgaaacttac ctgaagtcgg tatccggctg tttttccaga tataatcgcc tgtcagattg
     7381 atatgttccc aacctaatgg tgataaatgc gataatagtt gagcatttat tttcatgccc
     7441 ttacgcttca gagcatcaac cgctctttcc atgtaaactg tgttccataa ggaaatagcc
     7501 gccgtcaata aagtcaatcc actggcgcga taactttgat tttccggttt ccgatctcta
     7561 atctccccca atcgatgcat gaatacagca cgggccagag cattacgggc ttctcctttg
     7621 ttcagcccgg cctggacacg tcgccgcaat gccggatccc gaaaccagtc cagcataaac
     7681 agtgttcgtt ctatacggcc aatctctctc agagctttag ccaacccatt ttgtttgtaa
     7741 gcgtgcagca agaaccgtat tgacgggaat gtgttattca gtcggcagtg ctacgcgcca
     7801 ggggagcagt tcgccgaccc ggtttatcgg ccagtcggct atgacgtcaa ggacatagcg
     7861 gaggtagctt tctggctcca ctccgttcag tttgcacgtc ccgatcaggc tgtacagcag
     7921 cgctccccgc tctcctccat gatccgaacc gaagaacagg tagtttttgc ggcccagact
     7981 gaccatccgc aacgcatttt cagcgatgtt attgtccgcc tcagcccagc catcatctgc
     8041 atagtacgtc ag
//
LOCUS       DAZOXR010000189         8014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZOXR010000189 DAZOXR010000000
VERSION     DAZOXR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.195"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(53..442)
                     /locus_tag="ACHUNN_003653"
     CDS             complement(53..442)
                     /locus_tag="ACHUNN_003653"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0577922.1"
                     /translation="MSKYNPNQFYKFDVRISQLSEEINAAARLIYMVMVHSCDFYEEK
                     YKSYSPSIDHLAYMAGVSKRTAIRAIETLEKHGLIQVQKNQGRQSTYRVFTLDDKPEL
                     LVRPTSQEVAASVRQNDTTTSDKMSWG"
     gene            complement(806..1468)
                     /locus_tag="ACHUNN_003654"
     CDS             complement(806..1468)
                     /locus_tag="ACHUNN_003654"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR0577923.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            complement(1837..2388)
                     /locus_tag="ACHUNN_003655"
     CDS             complement(1837..2388)
                     /locus_tag="ACHUNN_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0577924.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            2975..3322
                     /locus_tag="ACHUNN_003656"
                     /pseudo
     CDS             2975..3322
                     /locus_tag="ACHUNN_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            3375..3770
                     /locus_tag="ACHUNN_003657"
     CDS             3375..3770
                     /locus_tag="ACHUNN_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0577925.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            3811..4554
                     /gene="cmoA"
                     /locus_tag="ACHUNN_003658"
     CDS             3811..4554
                     /gene="cmoA"
                     /locus_tag="ACHUNN_003658"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0577926.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            4551..5522
                     /gene="cmoB"
                     /locus_tag="ACHUNN_003659"
     CDS             4551..5522
                     /gene="cmoB"
                     /locus_tag="ACHUNN_003659"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0577927.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(5687..>8014)
                     /gene="torZ"
                     /locus_tag="ACHUNN_003660"
     CDS             complement(5687..>8014)
                     /gene="torZ"
                     /locus_tag="ACHUNN_003660"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0577928.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1987 a         1946 c         1784 g         2297 t
ORIGIN      
        1 aatgttttat ctaataactt atctaaactc taacacgggt gacattttgt cactaccccc
       61 atgacatttt gtcactggta gtggtgtcat tttgtcttac tgaggctgca acttcctgtg
      121 atgtaggtct tacaagtaat tccggcttat catccagtgt gaatacacga taagtgcttt
      181 gacgcccctg attcttttgc acctgaatta aaccgtgttt ctcaagtgtc tcaatggctc
      241 tgattgctgt tctctttgat acaccagcca tataagcgag atggtcaata cttggtgaat
      301 atgatttgta tttctcttca tagaagtcac agctatggac catgaccata taaatcaatc
      361 ttgctgctgc gttaatttct tctgaaagtt gagagattcg cacatcaaat ttataaaact
      421 gatttgggtt atatttagac attcataaat tacctcatac gtgaaaaggc caaccggata
      481 aaaccgatta gcctgtgtga ttaaaatatc tgggggattg atggactgat aaagaagtcc
      541 atgcctgaaa cagtacgatg attaaaacgc tgaggtatac cctgaatgag aggttaatat
      601 acgccaacca tctgacacct ccccaagtgt taccacttca agacatgaac ttttttatca
      661 gcccatatag aaactataac agatgcgatt ataaattgca acaattattt cacagaatga
      721 ttactgattg gtcatttatt gttcacaggg atacttctta acatctgaat cacctgtctt
      781 gtaaatgacc atatttatga gaattttagc tcaacctttg agcaatcctg tatgacgtgc
      841 tcagaggcat tctgagagca tctgcaatgt acttaatggg ttttccttgc ctgtatagtt
      901 ttatgaagct gtcgagcgtc tcaggtgggt aaatggtggg tcttcctctt agtccaccac
      961 ggttaatgat ggttaacggt agcaatgtat cagcacagca aaccatctgt gtaatttcat
     1021 gcttacgtgg ttctttttta aaaacaacga ctgcacttgg aaacggtgac ctaatagtaa
     1081 gcctgttatg ccctgctggt ggaaggtatt taactgcacg atgaagaata cggatttcat
     1141 gacagtggtt attgctgtaa atgtgcctaa gccagtagct ggtgttggct ctatgcggta
     1201 tcaggaaagc tactaaatcc gcttcagacg cttttaagag gaacgacgga atatcgctgt
     1261 acggcgggtt acagaagacc ttttcactaa cccatgattg acggcttata tcatcggtga
     1321 atcgtggtaa cagcgtgtca tgctcagttg cagcaccatc tagactgaaa ataaattcac
     1381 ggtgtaagtt cttaaataac aaaaatggag ttctccagta atcagaaccc ccatgatgct
     1441 tcccgattaa aatatgctca attgacataa gaaataaaaa tttttattgc aatcactctt
     1501 gacaaggttt actctctttt aagagatgct taacaggttt tctaaacgga tatcctttct
     1561 gggatatcta tcttttatct taaaagtttt tacagatact ttaaagatat ctgtatatat
     1621 tctgtacagt aacttttcag agtctgaaaa tttttataaa aaatgactag ccctctttga
     1681 atctcatctt ccccgaatct tcgaaaatat ctgtctagtt gattcggtag aatctacctc
     1741 atgatgtata cgtgtttagt acccgtcaca ccagctatgt gacccccttg gcaaaaaagc
     1801 caaatatcag ctacatccat gtaactattc agtaacttat ttagcaagtc tgtaataagt
     1861 tgctctagag attcctagct cttcctgaat ctgcaacctc gttttacctt gtgaaataag
     1921 catctgtaca gcctctgaag aagcttttac aggtcttccc atatgtaccc cttcagcttt
     1981 agcacgttca ataccagcct ttctacgctc tgctatcaag cttctttcaa gctgtgccaa
     2041 cgttgacata atgcctaaca ttgcttcacc catagcgctt gataagtcaa atccttctct
     2101 aaggctgata actgtcacac ctttagcctg tagcgcttta accgttgata acacatcaat
     2161 tgtgtcacgt ccaagacggt ctacagctac cacaactacg gtgtcacctt ctcgtgcata
     2221 agctaacaaa ctgctgaaac cgtttcttaa agaagctttg acagaaccac tgacagcttc
     2281 atcagcgaac catttagcaa tgttgtaccc tgcctcttca atttgcattt gctggttagt
     2341 gatggattgg tcatctgtac tcaccctgca atatccaagt atcgccataa tgttcccctt
     2401 agaagtctca aaagttagtc tcataagctt agctgtctca taatccaaaa acaacctttt
     2461 gagactactt gagggtgcat tctaccgtta cgttgaaagt gtctcaaaag gacttcctta
     2521 tgagactggt taatagagcg ctatgaagat tcaggagggt aactataacc atcactttct
     2581 aactttcacg taggaaatcg ttaagtttct tgatagttac accatgtgac caactatcta
     2641 tatatcgtta tatagccctg tatatagcac tatatgtagt gtctggagag ggacgactaa
     2701 cacgatacag tttaccctga aaagagactt cacagattct ttatatattt caataagtta
     2761 tgctaaaaag agactatacc gatactgcat agaatttgtg taggattgca tagatacttg
     2821 gaaaaacacc gttaaattgc atagtcattc acctgatatt gtcctgtttt tacaggtagt
     2881 tgattcggat gttctaggaa tcatcctcat agttgattcg gttagttggg cttgtaccaa
     2941 cccctaatca acctcatagt gtatttagac cttgatgatt ttagacagcc gccctgttca
     3001 tgcagcacgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc
     3061 ggtacatgct gtactgacgg cgacaaatcc gctgattcgt tttatcggta gtgatgatat
     3121 gacgcaaaac cgggaattat ttcaggtctg gttacagaaa ttagcgcagt ggcatcagac
     3181 cactacgcct tatctttttt tacatacgcc agacattgcc caggccccgg aactggtaca
     3241 taccctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc
     3301 acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt
     3361 taaagggagt ttgtatggta agcgcgctat atgccgtttt aagtgcgttg ttattaatga
     3421 agttctcttt tgatgtcgtt cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg
     3481 gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccca
     3541 tcgcgattgt gctgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata
     3601 tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc
     3661 tgtttcgctg gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc
     3721 tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac
     3781 gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc
     3841 cctatcgcca gactgggcga ctggaccttt gatgaacggg tagctgaagt cttcccggat
     3901 atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg tatgttagcc
     3961 gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg
     4021 acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgacaac
     4081 tccccggcga tgattgaacg ctgcagtcgt catattgacg cctataaagc ccctacgcca
     4141 gtagacgtca ttgaaggtga tattcgcgat atcgccattg aaaacgcatc gatggtggtg
     4201 ctgaatttta ccctgcaatt cctggaacct tccgagcgcc aggcattact ggataaaatt
     4261 tatcaaggac tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat
     4321 gccaaagttg gtgaactgtt gttcaacatg caccacgact ttaaacgtgc caacggttac
     4381 agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct gaccgattcc
     4441 gtggaaaccc ataaagcacg cctgcataaa gccggttttg agcatagcga gctgtggttc
     4501 cagtgcttta actttggttc actggtggca ttaaaagcag aggacgctgc atgatcgact
     4561 ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctc gaaacgctgc
     4621 ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag cagtggtcca
     4681 acgcggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg catagcgtaa
     4741 ccgccgaaag cgaagagcca ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc
     4801 ggaacctgat gccatggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg
     4861 aatggcgttc cgactggaaa tgggatcgtg ttctgcccca tctttctgat ttaaccggac
     4921 gcaccattct ggatgtcggc tgcggcagcg gttatcacat gtggcgcatg attggcgcag
     4981 gcgcgcacct cgcggtgggg atcgacccta cgcaactgtt tctgtgccag tttgaagcgg
     5041 tgcgtaaact actgggtaac gatcagcgcg cacatttgtt accgttaggt attgaacagc
     5101 ttccggcact gaaagccttt gataccgtct tttcgatggg cgtgctttac catcgccgtt
     5161 cgccgctgga gcatctctgg cagttgaaag atcaactggt gaatgagggt gaactggtgc
     5221 tggaaacgct ggttattgat ggcgatgaga acacggtgct ggtaccaggc gatcgttacg
     5281 cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga
     5341 agtgtggttt tgtagatatc cgcattgtgg atgtgtgcgt taccactact gaagagcagc
     5401 gacgcactga atggatggtt accgaatctc tgtccgattt cctcgaccca catgatccga
     5461 gtaagacagt tgaaggttat cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt
     5521 aaaggtctgg tattactgcc ggatgcggcg tgaatgcctt atccggccta caaacccttg
     5581 ctaattcaat ctattgcagg gactatgtag gcctgataag catagcgcat caggcagttt
     5641 tacgtttgca taacctcagc gcccgtttcc gggcgctatt cacttcttac gcctgaacag
     5701 caggcttatc aaatgccgtt aacttcggtg cgttacccgt atatttttcg atatacacca
     5761 gcgcagagtt accggcacag gcatttgcca gctgcgagct ggggatatcc gccgttaaca
     5821 cgttcgcact gccgttttta cacaagccgt tttccagatc tggccatgca ccttcatgca
     5881 cgcataccac gccttttttg atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac
     5941 gtttgttcca gacacgcacc agatcgccat tcgcaatact aaatcgagca gcatcttcgg
     6001 tgtgaatagt gatcggttca cgatcggcaa ccgcatattt tttacgcagt tccgcatagt
     6061 taagctgact gtgtaaacgg tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt
     6121 cggcggtacc cttccactca tcaggcgcaa gccaggttgg gtgtgccggg caatccttat
     6181 agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccacttggc gtacccagcg
     6241 catttttcac cggatcggcg cggaaatcac cataacgaac gtactgttcg ttcttctcgc
     6301 tgcggcgcat ttcgatcagt ttattttgct gccagaaggc attaaacatt ggcatagtga
     6361 cgcgttgcgc acgggcccct ttctgagcgg catcatagaa aaatttcagc cacgccattt
     6421 catctttacc ttcggtatag atctcttttc cgccaggttt gagtaattcc gcaagatcgg
     6481 caaacacatc aaaatcgttt cgcgcttcaa attgcggagc aacagcctgc ttcatcggca
     6541 caatatgctg gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg
     6601 tgatcggtaa tacgatatct gcgtgtttag cagctgcggt ccagtagcat tcagaaacga
     6661 cgatcatctc cggtttctgc catgctttaa tcagacgatt agtatcctgg tgatgggtaa
     6721 agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgc tctttaccgt
     6781 tatgctgata tttgccgcca ggattttcca gcgcatcgac gatacgggca acaggaatcg
     6841 ccctcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt
     6901 ccggcagcac gccgccaaca cgtgtcgggt tgccaccatt ggagtaatga taagagaaac
     6961 caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc
     7021 agtgtttctg ttcaccatat tgctggcgct gaattcccca gcctgccatc agcattgtgc
     7081 gattcgccgc catcagttca gcgagtttga ctatctgcgc ttccggtacg ccagtaattt
     7141 ccgccgccca ggccgcgctc ttcggcgtat tatcgctctt acctctcaga tactcttcaa
     7201 actgcggata cccggtagtg tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca
     7261 tcagggtatg cgcaatccct aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc
     7321 aggtggcatt atcgccaaag aagtcgatgg tttcggagcg gataggatca atggcaatca
     7381 ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca tcggtactgc
     7441 tccaggcaat ttttaaggta ttcagcgggt tcatccccca cagcaccaca acctggctgt
     7501 tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa ccgaccacat
     7561 gcggcatgat cacctgtgcc gccccggttg aataatcgcc gctatgcccg gaataaccgc
     7621 ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc
     7681 gccagccgta tgatccggca aaaatagccg atggtccgtt agctttgcga atgcgatcgt
     7741 gttgctcatg aatcagtttt aatgcttgtt cccagctcac ctgtacatag gtatcttcgc
     7801 cacgaccttt cgtcggttgc agtggattat cgagatagct ttttctcacc atcggatgct
     7861 gaatacgcgt cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggaa ttcggtatgg
     7921 ttttcgccag cgcgcctgtt gaagaaacaa tcttgccgtc cttcacttct acgttcatcg
     7981 ctccccaacg ttccgcggtg aggattttac cgtc
//
LOCUS       DAZOXR010000190         8010 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZOXR010000190 DAZOXR010000000
VERSION     DAZOXR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.196"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            44..568
                     /gene="radC"
                     /locus_tag="ACHUNN_003661"
     CDS             44..568
                     /gene="radC"
                     /locus_tag="ACHUNN_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0577929.1"
                     /translation="MNKLESLPGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYN
                     VRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEV
                     HPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVI
                     GRGEYVSFAERGWI"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUNN_003662"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUNN_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0577930.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUNN_003663"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUNN_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0577931.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUNN_003664"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUNN_003664"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0577932.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUNN_003665"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUNN_003665"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0577933.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUNN_003666"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUNN_003666"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0577934.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUNN_003667"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUNN_003667"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0577935.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="ACHUNN_003668"
     CDS             5387..6511
                     /locus_tag="ACHUNN_003668"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0577936.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUNN_003669"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUNN_003669"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0577937.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..>8010
                     /locus_tag="ACHUNN_003670"
     CDS             7317..>8010
                     /locus_tag="ACHUNN_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0577938.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGK"
BASE COUNT         2043 a         1851 c         2044 g         2072 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag
//
LOCUS       DAZOXR010000191         7968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZOXR010000191 DAZOXR010000000
VERSION     DAZOXR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.197"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUNN_003671"
     CDS             <1..239
                     /locus_tag="ACHUNN_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR0577939.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="ACHUNN_003672"
     CDS             279..1142
                     /locus_tag="ACHUNN_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0577940.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUNN_003673"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUNN_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR0577941.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     YGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="ACHUNN_003674"
     CDS             2679..4037
                     /locus_tag="ACHUNN_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0577942.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="ACHUNN_003675"
     CDS             4180..5130
                     /locus_tag="ACHUNN_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR0577943.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="ACHUNN_003676"
     CDS             5140..6522
                     /locus_tag="ACHUNN_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0577944.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6738..6812
                     /locus_tag="ACHUNN_003677"
                     /pseudo
     CDS             6738..6812
                     /locus_tag="ACHUNN_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUNN_003678"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUNN_003678"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR0577945.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1920 a         2019 c         2308 g         1721 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgactat ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAZOXR010000192         7924 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZOXR010000192 DAZOXR010000000
VERSION     DAZOXR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.198"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHUNN_003679"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHUNN_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUNN_003680"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUNN_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0577946.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUNN_003681"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUNN_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0577947.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUNN_003682"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUNN_003682"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0577948.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUNN_003683"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUNN_003683"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0577949.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUNN_003684"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUNN_003684"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0577950.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..>7924
                     /gene="fdhD"
                     /locus_tag="ACHUNN_003685"
     CDS             7101..>7924
                     /gene="fdhD"
                     /locus_tag="ACHUNN_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0577951.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQR"
BASE COUNT         1828 a         2053 c         2070 g         1973 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gttt
//
LOCUS       DAZOXR010000193         7776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZOXR010000193 DAZOXR010000000
VERSION     DAZOXR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.199"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUNN_003686"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUNN_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR0577952.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUNN_003687"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUNN_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0577953.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUNN_003688"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUNN_003688"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0577954.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUNN_003689"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUNN_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0577955.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUNN_003690"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUNN_003690"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0577956.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUNN_003691"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUNN_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0577957.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUNN_003692"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUNN_003692"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0577958.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAZOXR010000194         7732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZOXR010000194 DAZOXR010000000
VERSION     DAZOXR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.200"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            8..505
                     /locus_tag="ACHUNN_003693"
     CDS             8..505
                     /locus_tag="ACHUNN_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0577959.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYILYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            502..972
                     /locus_tag="ACHUNN_003694"
     CDS             502..972
                     /locus_tag="ACHUNN_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0577960.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1061..1573)
                     /locus_tag="ACHUNN_003695"
     CDS             complement(1061..1573)
                     /locus_tag="ACHUNN_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0577961.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1768..2532
                     /locus_tag="ACHUNN_003696"
     CDS             1768..2532
                     /locus_tag="ACHUNN_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577962.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2483..3886
                     /locus_tag="ACHUNN_003697"
     CDS             2483..3886
                     /locus_tag="ACHUNN_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0577963.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3886..5352
                     /locus_tag="ACHUNN_003698"
                     /pseudo
     CDS             3886..5352
                     /locus_tag="ACHUNN_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5243..5986
                     /locus_tag="ACHUNN_003699"
     CDS             5243..5986
                     /locus_tag="ACHUNN_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0577964.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5990..7210
                     /locus_tag="ACHUNN_003700"
     CDS             5990..7210
                     /locus_tag="ACHUNN_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0577965.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7214..7720
                     /locus_tag="ACHUNN_003701"
     CDS             7214..7720
                     /locus_tag="ACHUNN_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0577966.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         2059 a         1843 c         2105 g         1725 t
ORIGIN      
        1 gtgaataatg tcgccatcat tacgcaaggc tgttgcagtt gctattggtg gcggggctgt
       61 tgctatagca tctgtgttaa tcactggccc aggtggtaac gatggtctgg aaggtgtcag
      121 ctacatacta tacaaagata tcattggtgt atggactgta tgtcacggac acaccggaaa
      181 agacatcatg cctggtaaaa cgtataccga agcagaatgc aaagctctcc tgaataaaga
      241 ccttgccact gtcgccagac aaattaaccc gtacatcaaa gtcgatatac cggaaacaac
      301 gcgcggcgct ctttactcgt tcgtttacaa cgtgggcgct ggcaatttca gaacatcgac
      361 gcttcttcgc aaaataaacc agggctatat caaaggcgca tgtgaccagc tatgtcgctg
      421 gacatatgct ggcggtaagc aatggaaagg gctgatgacc cgtcgtgaga ttgagcgtga
      481 agtctgtttg tgggggcagc aatgagcaga gtcaccgcga ttatctccgc tctggttatc
      541 tgcatcatcg tccgcctgtc atgggctgtt aatcattacc gtgataacgc cattacctac
      601 aaagagcagc gcgacaaaaa cgcaagagaa ctgaagctgg cgaacgcaac cattactgac
      661 atgcagcagc gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct
      721 gatgcgaaag ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc
      781 gtcaaaggaa aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc
      841 aatgatgcca ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac
      901 ggaatcatca gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc
      961 cttggggggt agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga
     1021 atgggggaag ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca
     1081 cgaagctaaa actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg
     1141 tatacacttt cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca
     1201 gcacggcgat aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc
     1261 gaacccgttc tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc
     1321 caattattat accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg
     1381 tgattccagc ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca
     1441 taattgatgc tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta
     1501 taaaaagaag cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta
     1561 tccactccat caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga
     1621 cttaatttag gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg
     1681 gagcgtcgca ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga
     1741 taacagtaaa tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg
     1801 gcataccggg ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa
     1861 ggggctatca ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt
     1921 tgcaaaaatg gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc
     1981 cttacacaaa aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc
     2041 ggaatgcgta ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa
     2101 cacggtggtt atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg
     2161 ttgacgctcg aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat
     2221 attggtcggt ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg
     2281 attgaaaata tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc
     2341 atcgttggca cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc
     2401 gccactgata aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat
     2461 ggcaatggag agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc
     2521 ggctttacgt agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc
     2581 atcgtcaaaa tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct
     2641 gcgtttttgt tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct
     2701 caaaattcag atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat
     2761 cattaaccgg gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga
     2821 aattaagtca ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga
     2881 ataccagtgg aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat
     2941 attcaacccc ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga
     3001 aggcactctc atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct
     3061 taaggttatc gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg
     3121 cgttccggag tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt
     3181 ggatgcgcac aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt
     3241 ggctgacagt ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg
     3301 ggccgacgaa tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca
     3361 ggcggcgctg gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc
     3421 cggtgctaaa ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg
     3481 tgtgaattac cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa
     3541 cggcatcccc aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc
     3601 tgaccgtttc agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga
     3661 gctgatcagc atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac
     3721 acctcatcgt gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc
     3781 aaaacgcgag ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat
     3841 cgatacatcg ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa
     3901 caagccctgc ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc
     3961 gtagggatgc agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt
     4021 cgtaaccgca tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc
     4081 gatgctatcc cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca
     4141 aaagatcgtg gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat
     4201 gacgtgctga aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg
     4261 taggcaccaa tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt
     4321 ctcccgctcg accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg
     4381 ccggacctgg gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc
     4441 tggcgcattc atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag
     4501 aagatgaccg agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc
     4561 gcgttcgaca gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc
     4621 tacagcgtgg agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg
     4681 ctgaagaata tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac
     4741 tcgcgggata agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg
     4801 cagtttgtag aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag
     4861 tccccgaaag gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt
     4921 tcgttgcagg agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt
     4981 tcggaacttg gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa
     5041 atgtctgatg tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg
     5101 ctgggtgatg gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac
     5161 gtaaccggca tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg
     5221 ccgcccggca tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta
     5281 tcgaaccagc ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga
     5341 tggttcaaat gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat
     5401 caccccctgg gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga
     5461 gaaagtttgg cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag
     5521 cgctcctgtg ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc
     5581 tctgcctctc gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt
     5641 tgtcaccggt ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc
     5701 taagtctagg gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga
     5761 tatggcccga gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg
     5821 cgatgtccga gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc
     5881 aactttggac ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga
     5941 gatcgtgttt gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt
     6001 ttttgagacc aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa
     6061 agacgtgccg ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact
     6121 gaaacccgat aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc
     6181 ggccacgctt gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa
     6241 cgggaatgtg cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca
     6301 gaatgtccgg cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa
     6361 agacgaaagc gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga
     6421 cgcggagtac gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca
     6481 tgtggctctt gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac
     6541 aatggcaaat caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga
     6601 tgccgacacc atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg
     6661 ggatttgccg cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga
     6721 caaagcacca gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt
     6781 actgaaagcc ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc
     6841 tgacgataat ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga
     6901 ctcagcctgg cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc
     6961 gaccgcgttc aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt
     7021 cggtgatgcg gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt
     7081 gtctgaggtt gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa
     7141 catgggcatg accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg
     7201 taaagggtaa aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc
     7261 tgtctctcgc ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt
     7321 cgctgcctat ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga
     7381 tggtgatacc gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca
     7441 gccggacatg gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg
     7501 tggctacgtg accgttaatc tcggttctga ttttgatgcc agcactatca aaaaaggcga
     7561 tccggtatac gttgtcgtct ccactgatga atccatcaaa gtgccgctgg gtggattcat
     7621 gtccacgtca gtcagtggca aaaacgtggt gctgaccaac gctgaattca caggtgccgg
     7681 tgatgctgac ggcaatgcag aaatttcctg gaagatttaa ggaacagacg aa
//
LOCUS       DAZOXR010000195         7715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZOXR010000195 DAZOXR010000000
VERSION     DAZOXR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.201"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUNN_003702"
     CDS             <1..643
                     /locus_tag="ACHUNN_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0577967.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUNN_003703"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUNN_003703"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR0577968.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUNN_003704"
     CDS             2474..2986
                     /locus_tag="ACHUNN_003704"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0577969.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUNN_003705"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUNN_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0577970.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUNN_003706"
     CDS             5579..5782
                     /locus_tag="ACHUNN_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0577971.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUNN_003707"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUNN_003707"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0577972.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHUNN_003708"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHUNN_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0577973.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2101 c         2020 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZOXR010000196         7676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZOXR010000196 DAZOXR010000000
VERSION     DAZOXR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.203"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..882
                     /locus_tag="ACHUNN_003709"
     CDS             <1..882
                     /locus_tag="ACHUNN_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0577974.1"
                     /translation="LRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGAR
                     LLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFI
                     RAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIV
                     HEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVI
                     TQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHE
                     QSPASRNFRIHLLNALR"
     gene            1017..2237
                     /gene="mlc"
                     /locus_tag="ACHUNN_003710"
                     /gene_synonym="dgsA"
     CDS             1017..2237
                     /gene="mlc"
                     /locus_tag="ACHUNN_003710"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0577975.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2362..3057
                     /gene="bioD"
                     /locus_tag="ACHUNN_003711"
     CDS             2362..3057
                     /gene="bioD"
                     /locus_tag="ACHUNN_003711"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0577976.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3010..4302)
                     /gene="clcB"
                     /locus_tag="ACHUNN_003712"
     CDS             complement(3010..4302)
                     /gene="clcB"
                     /locus_tag="ACHUNN_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0577977.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4461..5075)
                     /gene="dmsD"
                     /locus_tag="ACHUNN_003713"
     CDS             complement(4461..5075)
                     /gene="dmsD"
                     /locus_tag="ACHUNN_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0577978.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5118..5972)
                     /gene="ynfH"
                     /locus_tag="ACHUNN_003714"
     CDS             complement(5118..5972)
                     /gene="ynfH"
                     /locus_tag="ACHUNN_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0577979.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5974..6591)
                     /gene="ynfG"
                     /locus_tag="ACHUNN_003715"
     CDS             complement(5974..6591)
                     /gene="ynfG"
                     /locus_tag="ACHUNN_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0577980.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(6602..>7676)
                     /locus_tag="ACHUNN_003716"
     CDS             complement(6602..>7676)
                     /locus_tag="ACHUNN_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR0577981.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         1934 a         1968 c         2009 g         1765 t
ORIGIN      
        1 cttcgtcatc tgcgttactt tgttgctgtt gcggaagagc tgcatttcgg gcgcgccgct
       61 gcccgcctga atatttcgca accaccgcta agtcagcaga ttcaggcact ggagcaacaa
      121 attggtgccc gactgctggc acgaactaat cgcagtgtat tgctgacggc agcaggaaaa
      181 cagtttcttg cagatagtcg gcaaatcctg tctatggtag atgacgctgc cgctcgcgct
      241 gaaagactgc atcagggtga agcgggggag ttgcgcattg gttttacttc gtccgctcct
      301 tttattcggg cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc cgatgtccat
      361 ttacaaaccc gcgaaatgaa cactcgtgag caaatcgctc cgctcattga aggaacgctg
      421 gatatgggat tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc
      481 catgaaccgc ttatggcgat gatcccgcac gatcatcccc tggcaaataa cccgaatgta
      541 acgctggctg aactggcgaa agaacccttc gtcttttttg atccgcacgt cgggacaggg
      601 ctgtatgacg atattctcgg gctgatgcga cgttaccatt tgacgcccgt catcactcag
      661 gaggtgggcg aggcaatgac catcatcggt ctggtttccg ccggtctggg tgtttcaatt
      721 ttgcctgctt catttaaacg tgttcagctc aacgaaatgc gctgggtgcc gattgctgaa
      781 gaggatgcgg tttctgaaat gtggttggtc tggccgaaac atcatgaaca aagtccagct
      841 tcgcgtaact ttcgtattca tctgctgaat gctctcaggt gagggaaatt tcagcgaaaa
      901 agcccgaaaa atgtgctgtt aatcacatgc ctaagtaaaa atttgacgac acgtattgaa
      961 gtgcttcacc atagcctaca gattatttcg gagcgcgaaa atatagggag tatgcggtgg
     1021 ttgctgaaaa ccagcttggg cacattgatc aaataaagca gaccaacgcg ggcgcggttt
     1081 atcgcctgat tgatcagctt ggtccagtct cgcgtatcga tctttcccgt ctggcgcagc
     1141 tggctcctgc cagtatcact aaaattgtcc gtgagatgct cgaagcacac ctggtgcaag
     1201 agctggaaat caaagaagcg gggaaccgtg gccgtccggc ggtggggctg gtggttgaaa
     1261 ctgaagcctg gcactatctt tctctgcgca ttagtcgcgg ggagattttc cttgctctgc
     1321 gcgatctgag cagcaaactg gtggtggaag agtcgcagga actggcgtta aaagatgact
     1381 tgccattgct ggatcgtatc atttcccata tcgatcagtt ttttatccgc caccagaaaa
     1441 aacttgagcg tctaacttcg attgccataa ccctgccggg aattattgat acggaaaatg
     1501 gtattgtaca tcgcatgccg ttctacgagg atgtaaaaga gatgccgctc ggcgaggcgc
     1561 tggagcagca taccggcgta ccggtttata ttcagcatga tatcagcgca tggacgatgg
     1621 cagaggcctt gtttggtgcc tcacgcgggg cgcgcgatgt gattcaggtg gttatcgatc
     1681 acaacgtggg ggcgggcgtc attaccgatg gtcatctgct acacgcaggc agcagtagtc
     1741 tcgtggaaat aggccacaca caggtcgacc catatggtaa acgctgttat tgcgggaacc
     1801 acggctgcct cgaaaccatc gccagtgtgg acagtattct tgagctggca cagctgcgtc
     1861 tcaatcaatc catgagctcg atgttacatg ggcaaccgtt aaccgtggac tcattgtgtc
     1921 aggcggcatt gcgcggcgat ctactggcaa aagacatcat taccggggtg ggcgcgcatg
     1981 tcgggcgcat tcttgccatc atggtgaatt tatttaaccc acaaaaaata ctgattggct
     2041 caccgttaag taaagcggca gatatcctct tccctgtcat ctcggacagc atacgtcagc
     2101 aggcccttcc tgcgtatagt cagcacatta gcgttgagag tactcagttt tctaaccagg
     2161 gcacgatggc aggcgctgcg ctggtaaaag acgcgatgta taacggttct ttgttgattc
     2221 gtctgttgca gggttaacat tttttaactg ttctaccaaa atttgcgcta tctcaatttg
     2281 ggccaggaaa gcataactta gactttcaag gttaattatt ttcctagttt atatttgtga
     2341 agcataacgg tggagttagt gatgctggag cgtttcttta ttaccggtac agacacttct
     2401 gtagggaaaa cggtggtttc ccgcgcattg ctacaagcgt tagcctccca gggaaaaaca
     2461 gttgcgggat ataaacccgt agcgaagggg agcaaagaga cacccgaagg gctgcgtaat
     2521 aaagatgccc tggtgttgca gagtgtttca accatcgaac tgccttatga agcagttaat
     2581 cctatcgcgt taagcgaaga agaaagtagc gtggcgcaca gttgcccaat caattacacc
     2641 ctcatttcaa acggcctggc aaacctgacc gataaagtcg atcatgtcgt ggtagaaggg
     2701 actggcggct ggcgcagtct gatgaatgat ttgcgtccac tctctgaatg ggtggtgcag
     2761 gaacagttgc cggtgttgat ggttgtcggt attcaggaag gttgcattaa ccatgcactg
     2821 ctaacagctc aggcgattgc caacgacggg ctgccgctca ttggctgggt ggctaaccga
     2881 atcaacccag gactggcgca ttatgcggaa gtcattgatg tgctgggtaa aaaacttccg
     2941 gcaccgctca ttggtgaact gccttatctg ccgcgcgccg aacagcgaga actggggcaa
     3001 tacatccgct tagctatgct gcgcagtgtg ctggcggtag atagagtcac ggtgtaacgt
     3061 ccgcgaaatt accgacgcaa tgacgcaggc aatcaataaa ccggggagta gctgatactc
     3121 cccggtcatt tcacatatca tcaacgtcga cataatcggc gcgtgcgtgg ttgccgccaa
     3181 cagtgtcgcc atcccggtca atccgagtaa aagcgtaatt tcttcgccat cagggaacca
     3241 taatcccagg ctacgaccat acaacatgcc aatggcaaga ccgataaata gcgtcggtgt
     3301 aaagacacca ccgggtgcgc cggaaccgct actcgccagc acggcacaca gtttacaaag
     3361 gaagatcccg gcaatgatca ttaacagtgg tggggcggtt aaaaaggatt gtacggtgct
     3421 atagccgttg ccccacactg caggtgtgaa cagggaaagc agacccacga tcaacccgcc
     3481 tagtgccagt tgccagggcg gcgcaagttt gagactcaca aatccacgat gacaggcgtt
     3541 cattaacgtt aacaacagtg gtccgcacag acctgccagc acacctgtac tgataatcag
     3601 cgcatagtca ctagcctgaa ccgttactga gagttgtacg ctgtagagta acgcgtcgct
     3661 atgattaata agattgctaa ccagcaacgc cacgacggcg gaaataatca ccgggccgag
     3721 agaggccaac atcatagtgc caaacagcac ttcggctata aataaactgc cagcaagcgg
     3781 ggcgcgatag gccgcagcca ttcccgccgc ggccccacag gcgatccata atttccattc
     3841 ctggcgtggc gtaaaacgtt gggcaaaaca ggaggcggca agggcagcta aaagaatcat
     3901 cgcaccttcg cgaccaattg cactgccgct ggttactacc agcagcgagg caagcgattt
     3961 aaccaggctt gctgcgtaat cgaactgtcc atcggtttgc aacgcttcca tgtaatcggt
     4021 cggcgcatga gggcgttgtt gggtaaattt ctgccagccc atcagcaaca aacccgccgc
     4081 cagtccgccg agcgccggag ttagcagccg tcgccagggg gaaaggtttg tcgctgcatt
     4141 gaccagactg ccggagtcat tattgaggaa caaccactcc agtagcagca tcgcatgacg
     4201 aaacccggca acggcaaagg ccgcgagaat accgacgact gttgcgataa gcagacggcg
     4261 gaacatagtg cgcagatccg gatagatgtg tagatgatgc atgagcagaa atgggcaatg
     4321 aaacacgggc gactattttg cggcaaaaga ggccaaccgc aaaagcagtg cgtgctttaa
     4381 gagataaaaa aacgtgacat tgtaacttat tattgccgaa tcgcgcgtaa tcgtcaccat
     4441 ccggcaatat tacggtgatc ctatcgaaag agtggtttaa ccgcgacagg aatcaacagt
     4501 tgtgattgcc attgcgccag cgttaagcga gccagttcac ccagtgcacg gtagaaaggg
     4561 tgttctgctt tttcgataaa aacatcaaga aaacgtgttg accacggaaa aaggtgccat
     4621 gccagcaatt cttcgcattc tgtctggcga ccattctccg ccaaccacgc agccatcaac
     4681 agcagtgaac caaaatgatc ttccggttcg ttttgcttca tttcaaactg aatgcctttc
     4741 tcgcgcatcc actgacgaag tgccaatgtt gaatcgccaa acagcacaga ttcgcgatcc
     4801 agccagaccg atccccacgg cggagacggc agtgcccacg ggccgacaaa caaacgctgc
     4861 caggcctggg cgtgagtctc ttcacactgt gtctgaaaag ccgtcaccag cggcgctaat
     4921 gacgcttctg gtagaggcca ctgagtttcc cagccgtcac tggtgagtac cgcaaccaga
     4981 ggtgcggctt ccgcactctc tggagcgtaa taaaacagcg cgcccaacac gcgcgccgcg
     5041 acagaaaaat tatcttgctg tgaaaaatgg gtcattccta acatcctgaa atgtgcgggt
     5101 tgccccgcac caatttgtta acctgcaata gccattccta ctgtcatgtg caggccataa
     5161 aagagcacac gcccgatcat ctcaccaaca agtatgagga ttagccccag aacaagtccg
     5221 gcaacgtgag gttcacggcg acgaatgagc ggacaaagcc agcatccaag acctgcgcat
     5281 aacaatacca cacgccagac ctgtaaggag gcgtaatctg gtaccagtgc gctggcttgt
     5341 tgcacggatg agtgaataga cgccagagac aaaccttgca gcacaatcac cccggcacaa
     5401 gcgatcagcg cgagaacgct aatgatggca aatggtgtgg tattaaatgt aacgcgggct
     5461 gcccgcagaa ttgcggcagc aagtattgga ccgctcaaca ataccgtcag gaagaaagcc
     5521 agcgtagtgt aaccgttatg ccaggttggc acagtgtcga tttgatacac gcaggtcatc
     5581 atccagacga aaatgacgcc gagcgccatg ctgacaagta accagagttt ccccaacgct
     5641 tgcggcattt taccgatgac cgccaccagc caccatagtc cgccaacggc aaaaaatatg
     5701 gaaccggcag caatctcgtt actcagacca gaagctccga ttcgattcag cgagttaaac
     5761 gcccgcagtg gcgatcccag gtgcatgaca gaggccataa acccgatgcc catcaacagc
     5821 cagaggaaaa acatgccacg gacaatacgc tgacggtctg cgtcattttt ggcggcgaac
     5881 cagccaattc cgctaacaat cagcgcgcct actacgcatt gacccaatac ggtaaagata
     5941 accagtggcc actcatgcca tccatttccc attttacact tcctccggat tagccagata
     6001 accagtggta tcgctggtcg ggcggctgtt ggcgttaggt ttgataacga tattgggttt
     6061 tgtgaaatgc gcgcgcggca gtggcgcgac ggcagccaga gtgccgtgct tctggcgcag
     6121 ctcttcaatt ggaccgaact caagtgcacg cagcgggcag gattcgacac atatgggttg
     6181 tttcccctcg gcgacgcgcg aataacaacc atcgcacttc gtcatgtgcc ctttttcagc
     6241 attgtactgt ggcgcgccgt acggacaagc catgtggcag tagcggcagc cgatacaaac
     6301 atcttcatca accaccacaa agccatcttc acgcttatgc attgcgccgc tggggcagac
     6361 ttttgtacat gcggggtcat cgcaatggtt acaggagatg gagagataat aagcgaacac
     6421 gttctggtgc cagacgccat tatcttcctg ccagtcgccg ccagcatatt cataaatacg
     6481 gcggaaactg acttccgggc caagatcttt aaaatctttg cacgccagtt cgcaggtttt
     6541 acagccagta cagcggctgg aatcgataaa aaatccatat tgtgtggtca cgggctactc
     6601 cttaaacctt ttcgatctgg acaagattgc tgtgcgacgg gtttcccttt gccagcggtg
     6661 aagggcggtg agaggtcaga atattgatac tgccgccgtg atcgacccgg tcaccaaaca
     6721 tatccgcttt aagccaagca ccttgcccga tggcggtaac gccaggcaga atacgcggag
     6781 tcacttttgc ggcaatcagc atttctccat tattgttaaa tacccgcacg gtatcgccat
     6841 gacggatacc gcgtgcctga gcatcaatgg ggttgatcca cacctcttgt gggcaggcct
     6901 gctgtaacac atcaatattg ccgtagctgg agtgggtacg cgctttgtaa tggaagcccg
     6961 ttaactgcag tggataggtt ttccgcaggg gatcgtccca gccatcaaaa cctggggtat
     7021 acgcaggaag gggatgaata acttcatctt ttttcaattc ccaggtatcc gcaatcttcg
     7081 ccagtcgttc agaataaatt tcgattttcc ccgaaggtgt tttcaacggg tttgcctgtg
     7141 gatcttcacg gaatgcgcgg aaagcgacgt agtgttcttc cgggcatttt ttcttaaaga
     7201 tcccggtcgt tttcatctcc tcgtagtcgg gcatctcagg gttacgttcc ttcgttttcg
     7261 catggagata tttgatccat tcatgctgac tgcgaccttc agtaaaggtt tgataaacgt
     7321 ctggtcctaa gcgtttggcg acttcactca gcatccagta gatgggtttg cgttcaaatt
     7381 ttgctgaggt tgcgggttgg gcgaggatca catagcccat attccctgca gattcatgag
     7441 agataaggtc ttcttgctct gttggcatca ggtcgggcaa caggatatcg caatacttag
     7501 ccgaggccgt catgaagtgg tcaatgccaa caatcatctc gcacttgctg tcatcctgaa
     7561 gcacctcatg ggtgtgattg atgtcgccat gttgattgat caatgtgtta ctggcgtagc
     7621 accataaaaa cttgatgggg acatccagtt tttcttttcc acgaacacca tcacgg
//
LOCUS       DAZOXR010000197         7627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZOXR010000197 DAZOXR010000000
VERSION     DAZOXR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.204"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHUNN_003717"
     CDS             complement(152..487)
                     /locus_tag="ACHUNN_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0577982.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHUNN_003718"
     CDS             complement(491..835)
                     /locus_tag="ACHUNN_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0577983.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUNN_003719"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUNN_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0577984.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUNN_003720"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUNN_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0577985.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHUNN_003721"
     CDS             1257..1376
                     /locus_tag="ACHUNN_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0577986.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUNN_003722"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUNN_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0577987.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUNN_003723"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUNN_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0577988.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUNN_003724"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUNN_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0577989.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHUNN_003725"
     CDS             4176..4544
                     /locus_tag="ACHUNN_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0577990.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUNN_003726"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUNN_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0577991.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHUNN_003727"
     CDS             5295..5588
                     /locus_tag="ACHUNN_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0577992.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHUNN_003728"
     CDS             5660..6319
                     /locus_tag="ACHUNN_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0577993.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHUNN_003729"
     CDS             6411..6857
                     /locus_tag="ACHUNN_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0577994.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7627)
                     /gene="hlyE"
                     /locus_tag="ACHUNN_003730"
     CDS             complement(7064..>7627)
                     /gene="hlyE"
                     /locus_tag="ACHUNN_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0577995.1"
                     /translation="IKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1959 a         1714 c         1772 g         2182 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaat
//
LOCUS       DAZOXR010000198         7529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZOXR010000198 DAZOXR010000000
VERSION     DAZOXR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.205"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..896)
                     /gene="waaH"
                     /locus_tag="ACHUNN_003731"
     CDS             complement(<1..896)
                     /gene="waaH"
                     /locus_tag="ACHUNN_003731"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR0577996.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
     gene            complement(1135..2160)
                     /gene="tdh"
                     /locus_tag="ACHUNN_003732"
     CDS             complement(1135..2160)
                     /gene="tdh"
                     /locus_tag="ACHUNN_003732"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0577997.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(2170..3366)
                     /gene="kbl"
                     /locus_tag="ACHUNN_003733"
     CDS             complement(2170..3366)
                     /gene="kbl"
                     /locus_tag="ACHUNN_003733"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0577998.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            3580..4512
                     /gene="rfaD"
                     /locus_tag="ACHUNN_003734"
     CDS             3580..4512
                     /gene="rfaD"
                     /locus_tag="ACHUNN_003734"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0577999.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4522..5568
                     /gene="rfaF"
                     /locus_tag="ACHUNN_003735"
     CDS             4522..5568
                     /gene="rfaF"
                     /locus_tag="ACHUNN_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0578000.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5572..6543
                     /gene="rfaC"
                     /locus_tag="ACHUNN_003736"
     CDS             5572..6543
                     /gene="rfaC"
                     /locus_tag="ACHUNN_003736"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0578001.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(6613..>7529)
                     /locus_tag="ACHUNN_003737"
     CDS             complement(6613..>7529)
                     /locus_tag="ACHUNN_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0578002.1"
                     /translation="LFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRV
                     ALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAIL
                     VFPFFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYS
                     HDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLH
                     NDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSK
                     PVPTAVFVTIILLCAYFKAQSDQCLLDK"
BASE COUNT         2001 a         1854 c         1807 g         1867 t
ORIGIN      
        1 ataccggaag taaatatctc ggcaatcatc cgttggcggg taataatatc cggctctttg
       61 cgcaccgcat ggcaaacacg caatgcttcg taagttattt gctgatgaaa ttccggataa
      121 atcgtaattt tgtcggcata atttcgattt aatttctcta gcagacgagt aatcttaata
      181 tagtgacgtt gataattaag gtttttgttc ccttgtctat gcaaccgact cactgacgta
      241 ttatgcagat aatatttata taacgattgc tcggtatatc gcgcacgcag cgcattaaac
      301 ataaattctg ttgtccagac aatatcctga tgatgtaatc cggcaataaa tttaatgtta
      361 tttttaacaa taacatcgcg gcgataaacc cccatccaga caacgtgagt ccaacggcgc
      421 gaagaaagcc ccatccgcag ccagtccggg ccggttaata cgccggttga gcgaaggcga
      481 tcgctgggga tggatttcca ggtttctccc gtttcacgaa aacaccagtc agcgttgcac
      541 tgcgccacgt cgaggtcgtc ctctaacgcc atggtcatca gcgtttcgta catggtggga
      601 tagacttcat cgtcagcatc gacaaaagcg acatatttgc ccgttgccac ttcaatccca
      661 cgattacgcg ccaccgatgc gccagcattc gcctgatgca acaaacgaac gtgcggatag
      721 ttttctgcgt aatgctttgc tatttcaaca gaattatccg ttgaaccatc gttaataata
      781 atgatttcca gagcagtcca ggtttgcgta attaaagatt ccatacaagt gcggaaatca
      841 tcgcccgcat tatataacgg aataataaca ctaagtttat tggtgctgtt catcataaat
      901 taccgacgac ctctgaagtt atcctgacgt tttacaggct ttgtattaag aaactattaa
      961 gcctgtgaga tattattttt acaggtgaga gagtcacttc agggtagaga gataatgaga
     1021 gcagctatat aagagatcag cgcaattacg atatctttat tacactgaca ggattttaca
     1081 tatttaacgg attatcctca ggcaaaaggg ctggaatacc agcccttgtt cgtgttaatc
     1141 ccagctcaga attactttcc cggactggcc cgaacgcata gcgtcaaagc ccttctggaa
     1201 atcatcgata gagaaacgat gggtaatgat cggcgagaga tccagacctg actgaatcag
     1261 cgccgccatc ttgtaccagg tttcaaacat ctcacgaccg taaatacctt taatgaacaa
     1321 gcctttaaag attactttgg tccagtcgat agacatatcg gacggtggaa tacccagcat
     1381 cgcaatgcga ccgccgtggt tcatggtgtc gagcatggta cgaaacgctg gcggcgcacc
     1441 ggacatttcc agaccgatat caaagccttc ggccatgccc agttctgcca tcacatcatt
     1501 aaggttttct ttagcgacgt tgaccgcacg ggtgataccc attttgcgcg ccagttcgag
     1561 acgatattcg ttaacatcag tgatcaccac attgcgtgca ccaacgtgtt tcgccaccgc
     1621 cgctgccata ataccaatcg ggcctgcacc agagaccagc acatcctcgc caaccagatc
     1681 gaacgacagc gcggtatgca cggcgttacc gaagggatca aaaatggaag ccaggtcgtc
     1741 ggagatattg tcgggaattt tgaaggcgtt gaacgccgga atcaccagat attcggcgaa
     1801 gcagcccggg cggttaacgc cgacgccgat cgtgttgcgg cacagatggg tacgaccgcc
     1861 acgacagttg cgacagtgac cgcaggtgat atggccttcg ccagaaacgc gatcgccaat
     1921 cttgaagccc ttcacttcct gaccaatacc taccacttca ccgacatatt catggcccac
     1981 gaccatcggc accgggatgg ttttttgcga ccactcatcc cagttataga tgtgaacgtc
     2041 agtcccgcag atggctgttt tacggatttt aatcagcaga tcgttatgcc cgagttccgg
     2101 tacaggaacg tcggtcatcc agatgccctc ttccgctttc agtttggata acgctttcat
     2161 ctcacatcct caggcaataa cgcccagttg tttaccaata cgcgtgaatg cttctactgc
     2221 acgcgtaatt tgctcagggg tatgcgccgc agacatctgg gtacgaatac gcgcctgacc
     2281 tttcggaacg accggataga agaaaccggt gacgtaaatg ccctcttttt gcagctcacg
     2341 ggcaaatttc tgcgccacta ccgcatcacc aagcatgacc ggaataatgg cgtgatcggc
     2401 tcccgccagg gtaaagcccg ccgccgacat ttgctcacgg aactgacgcg cgttcgccca
     2461 cagacggtca cgcagctcgc tacccgcttc gaccatctcc agtactttga tggatgcggc
     2521 aacaatggcc ggtgccagcg agttggaaaa caggtacgga cgagaacgct ggcgcagcca
     2581 ctcaaccact tctttgcgcg ccgcggtata accaccagaa gccccgccca gcgctttacc
     2641 gagcgtgccg gtgataatat cgacccggcc catcacatcg cagtattcat gggaaccacg
     2701 accattttca ccgacaaaac cgaccgcgtg ggagtcgtct accatcacca gggcatcata
     2761 tttatctgcg agatcgcaaa cgcccttcag gttggcaatc acgccgtcca tcgagaacac
     2821 accatcggtg gcaatcagca catgacgcgc accggcttca cgcgcttctt tcagacgtgc
     2881 ttccagctcc tgcatatcgt tgttggcata gcgatagcgt ttagctttac acagacgcac
     2941 accatcaata atagacgcgt ggttcagtgc gtcggagata atggcgtctt ccgcgcccag
     3001 cagcgtttca aacaggccac cgttagcatc aaagcaggaa gagtagagaa tcgcatcttc
     3061 catccccagg aatgccgcca gtttttgttc aagctcttta tggctgtcct gagtgccgca
     3121 aataaaacgc accgaagcca tgccgaaacc gtgagaatcc attcccgcct ttgccgccgc
     3181 aatcagatcc ggataattcg ccagcccgag atagttattg gcacaaaagt taatgacgtg
     3241 gcttccatca gccacagtga tatctgcttg ctgcgcagac gtaataatgc gctcttcttt
     3301 aaacaaccct tccgcccgtg cggtttccag atcgttggtt aactgctgat aaaattctcc
     3361 acgcattgcg attctccaga cagggcaaat tccagcacat attacccaaa cttataggtc
     3421 gcgacgagat gacgcgttaa cacttctgca aaattcagga taaataacgt ccatcaatct
     3481 cacttttggc gaatggctga agccaacgcc gtgtagtgat atgatagaag tattcgtgtc
     3541 tgagattgtc tctgactcca taattcgaag gttacagtta tgatcatcgt taccggcggc
     3601 gcgggcttta tcggcagcaa catcgttaag gccctgaatg ataaaggcat caccgatatt
     3661 ctggtggtgg acaacctgaa agacggcacc aagtttgtga acctggtgga tctggatatc
     3721 gcggactata tggataagga agacttcctg atccagatta tggctggcga agagttcggc
     3781 gatgtcgaag cgattttcca cgaaggcgcg tgctcttcca ccaccgagtg ggatggcaag
     3841 tatatgatgg ataacaacta tcaatactcc aaagagctgc tgcactactg tctggagcgc
     3901 gaaatcccgt tcctgtatgc ctcttccgca gccacctacg gcggacgcac ctccgacttt
     3961 attgaatccc gcgagtacga aaaaccgttg aacgtctacg gttactcaaa attcctgttt
     4021 gatgaatatg ttcgccaaat cctgcctgaa gctaactcgc agattgttgg cttccgctat
     4081 ttcaacgttt atggaccgcg tgaaggccat aaaggcagca tggcgagcgt cgctttccat
     4141 ctcaacaccc agcttaacaa cggtgaatca ccgaagctgt ttgaaggtag cgagaacttc
     4201 aaacgcgact tcgtctatgt gggcgacgtg gctgatgtga atctgtggtt cctggaaaat
     4261 ggcgtttccg gcatcttcaa cctcggtact ggtcgtgcgg aatccttcca ggctgtagct
     4321 gatgctacgc tggcttatca caagaaaggc cagatcgaat acattccgtt cccggataag
     4381 ctgaaaggcc gctaccaggc gttcactcag gcagatctga caaatctgcg cgcggcgggt
     4441 tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg aatacatggc ctggctgaat
     4501 cgcgacgcat aagagctctg catgaaaata ctggtgatcg gcccgtcttg ggttggcgac
     4561 atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc gctatcccca ggcgataatc
     4621 gatgtgatgg caccggcatg gtgccgtcca ttattatcgc ggatgccgga agttaacgaa
     4681 gctattccta tgcctctcgg tcacggagcg ctggaaatcg gcgaacgccg caaactgggt
     4741 catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct tacccaactc cttcaaatct
     4801 gcattagtgc ctttcttcgc gggtattcct catcgcaccg gctggcgcgg cgagatgcgc
     4861 tacggtttac tcaacgattt acgcgtgctc gataaagaag cctggccgct aatggtggaa
     4921 cgctatgtcg cgctggccta tgacaaaggc gtgatgcgta ccgcacaaga tctaccaaaa
     4981 ccgttgttat ggccgcaatt gcaggtgagc gaaggtgaaa aatcatatac ctgtaatcaa
     5041 ttttcgcttt catcagaacg tccgatgatt ggtttttgcc cgggtgcgga gtttggtccg
     5101 gcaaaacgct ggccacacta ccactatgcg gaactggcaa agcagctgat tgatgaaggt
     5161 tatcaggtgg ttctgtttgg ctcggcgaaa gatcatgaag cgggcaatga gattcttgcc
     5221 gctttgaata ccgagcagca ggcatggtgt cggaacctgg cgggggaaac acagcttgat
     5281 caagcggtta tcctgattgc agcctgtaaa gccattgtca ctaacgattc tggcctgatg
     5341 cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt atggtccgag tagcccggac
     5401 ttcacaccgc cgctatccca taaagcgcgc gtgatccgcc tgattaccgg ctatcacaaa
     5461 gtgcgtaaag gtgacgctgc ggagggttat caccagagct taatcgacat tactccccag
     5521 cgcgtactgg aagaactcaa cgcgctattg ttacaagagg aagcctgacg gatgcgggtt
     5581 ttgatcgtta aaacatcgtc gatgggcgat gttctccata cgttgcccgc actcactgat
     5641 gcccagcagg caatcccagg gattaagttt gactgggtgg tggaagaagg gttcgcacag
     5701 attccttcct ggcacgccgc cgttgagcga gttattcctg tggcaatacg tcgctggcgt
     5761 aaagcctggt tctcggcccc cataaaagcg gaacgcaaag cgtttcgtga agcgctacaa
     5821 gcagagaact atgacgcagt tatcgacgct caggggctgg taaaaagcgc ggcgctggtg
     5881 acgcgtctgg cgcatggcgt aaagcatggc atggactggc aaaccgctcg cgaaccttta
     5941 gccagtctgt tttacaatcg taagcatcat attgcaaaac agcagcacgc cgtagaacgc
     6001 acccgcgaac tgtttgccaa aagtctgggc tatagcaaac cgcaaaccca gggcgattat
     6061 gctattgcac agcatttcct gacgaacctg cctacagatg ctggcgaata tgccgtattt
     6121 cttcatgcga cgacccgtga tgataaacac tggccggaag aacactggcg agaattgatt
     6181 ggtttactgg ctgattcagg aatacggatt aaacttccgt ggggcgcgcc gcatgaggaa
     6241 gagcgggcga aacgactggc ggaaggattt gcttatgttg aagtattgcc gaagatgagt
     6301 ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg tagtgtcggt ggatacgggg
     6361 ttaagccatt taacggcggc actggataga cccaatatca cggtttatgg accaaccgat
     6421 ccgggattaa ttggtggata tgggaagaat cagatggttt gtagggctcc ggggaatgag
     6481 ttgtctcaat tgacagcaaa tgctgttaag cgattcattg aagaaaacgt tgctatgatt
     6541 taaattacaa ccccttaata gataattttg aaataaaatc agcctccttg ctgattttat
     6601 ttcacatatt cattatttat ctaataaaca ttggtctgat tgtgctttaa aataagcaca
     6661 aagcaatatt atggtgacaa acacagcagt cggaaccggt ttagaaaaga gtattacatc
     6721 acttaggcct actaccagta gtgaaaaaag caagattaaa atataaggtt ctttagcagt
     6781 tcttaaggca tatatgagta ttgctgaaaa ggctaaaatt gttaatacaa ctccaggaat
     6841 accacgcgtt gataacgtat ctatgagatc gttatgcaaa tgagaatcta tatagggtaa
     6901 agcgccactc aatctaggct ctttttcttc tagttcatgt attttttccg ctcgtttttc
     6961 cagtgattgt cctattggag aatatgtttt taagccaact tcatacattg ccagacgggc
     7021 accgactgaa gttctggtgt tatcatgcga ataattaact aaatcgttat ttaagttatt
     7081 cattctcata agcaatgtat ctttaaaaga aaatacacct gctaataatg caatcgcaat
     7141 aaaacaatat aacttataat taattcgctt attaatataa ctatccatta cgattaatag
     7201 taacgcaaag aatggaaaca ctaatatggc tgcacgcgtt tcagttacta tgacagcaca
     7261 taagctaagc atgaagttaa ttgtatataa tgttgtttta tgtctaaagt cggattttaa
     7321 aatgagaatt gatgccagta aagaagggaa aaggatgatg tagcctgctg ttgtagcgtg
     7381 ggaagctgat aatgcaacac ggtcaacgtt taaatagaaa tgttgccaca tggcatgcgc
     7441 aaaaaaaagt aactgcgttg ccagggatgc gaacagaatt agtttcccaa attttatttt
     7501 tgttctcaac tcatttttag caaaaagaa
//
LOCUS       DAZOXR010000199         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZOXR010000199 DAZOXR010000000
VERSION     DAZOXR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.206"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUNN_003738"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUNN_003738"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR0578003.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHUNN_003739"
     tRNA            647..722
                     /locus_tag="ACHUNN_003739"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUNN_003740"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUNN_003740"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR0578004.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUNN_003741"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUNN_003741"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0578005.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUNN_003742"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUNN_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR0578006.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUNN_003743"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUNN_003743"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0578007.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHUNN_003744"
     CDS             complement(5917..6249)
                     /locus_tag="ACHUNN_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR0578008.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUNN_003745"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUNN_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0578009.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2029 c         1869 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat gtcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZOXR010000200         7429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZOXR010000200 DAZOXR010000000
VERSION     DAZOXR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.207"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..99)
                     /locus_tag="ACHUNN_003746"
     CDS             complement(<1..99)
                     /locus_tag="ACHUNN_003746"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HFR0578010.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(124..1224)
                     /locus_tag="ACHUNN_003747"
     CDS             complement(124..1224)
                     /locus_tag="ACHUNN_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0578011.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="ACHUNN_003748"
     CDS             complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="ACHUNN_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0578012.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2372..2938)
                     /locus_tag="ACHUNN_003749"
     CDS             complement(2372..2938)
                     /locus_tag="ACHUNN_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0578013.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..4890
                     /gene="argS"
                     /locus_tag="ACHUNN_003750"
     CDS             3154..4890
                     /gene="argS"
                     /locus_tag="ACHUNN_003750"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0578014.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(4932..4976)
                     /gene="yecV"
                     /locus_tag="ACHUNN_003751"
     CDS             complement(4932..4976)
                     /gene="yecV"
                     /locus_tag="ACHUNN_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR0578015.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            5067..5429
                     /locus_tag="ACHUNN_003752"
                     /pseudo
     CDS             5067..5429
                     /locus_tag="ACHUNN_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5433..6503)
                     /locus_tag="ACHUNN_003753"
                     /pseudo
     CDS             complement(5433..6503)
                     /locus_tag="ACHUNN_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="ACHUNN_003754"
     CDS             complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="ACHUNN_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR0578016.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1947 c         1776 g         1786 t
ORIGIN      
        1 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat
       61 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
      121 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
      181 cagttcgttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
      241 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
      301 aagtttttca gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc
      361 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
      421 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
      481 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
      541 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
      601 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
      661 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
      721 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      781 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      841 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      901 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      961 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
     1021 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
     1081 agatttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
     1141 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
     1201 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accggaatca
     1261 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
     1321 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
     1381 agttgctatt tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg
     1441 aatataaaaa atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg
     1501 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
     1561 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
     1621 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
     1681 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
     1741 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1801 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1861 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
     1921 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
     1981 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     2041 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
     2101 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     2161 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
     2221 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg
     4441 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     4501 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
     4561 tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
     4621 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     4681 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     4741 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     4801 aacagccgtc tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg
     4861 ctgggtattg agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca
     4921 gggttacctg atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca
     4981 ttgttttctg actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat
     5041 aacggttgaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta
     5101 tctcttgcca ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca
     5161 tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat
     5221 gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt
     5281 atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta
     5341 acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa
     5401 tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg
     5461 gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5521 cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     5581 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5641 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5701 acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg
     5761 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     5821 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5881 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5941 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg
     6001 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6061 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6121 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6181 cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6241 agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6301 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6361 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6421 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6481 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6541 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6601 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6661 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6721 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6781 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     6841 gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc
     6901 tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc
     6961 atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa
     7021 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac
     7081 cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg
     7141 cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7201 tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg
     7261 gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat
     7321 gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgccagcat
     7381 ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtca
//
LOCUS       DAZOXR010000201         7320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZOXR010000201 DAZOXR010000000
VERSION     DAZOXR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.208"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            142..1206
                     /gene="ulaG"
                     /locus_tag="ACHUNN_003755"
     CDS             142..1206
                     /gene="ulaG"
                     /locus_tag="ACHUNN_003755"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0578017.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHUNN_003756"
     CDS             1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHUNN_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0578018.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHUNN_003757"
     CDS             complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHUNN_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0578019.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHUNN_003758"
     CDS             2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHUNN_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0578020.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHUNN_003759"
     CDS             3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHUNN_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0578021.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3867..5492)
                     /locus_tag="ACHUNN_003760"
     CDS             complement(3867..5492)
                     /locus_tag="ACHUNN_003760"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0578022.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5576..6739)
                     /locus_tag="ACHUNN_003761"
     CDS             complement(5576..6739)
                     /locus_tag="ACHUNN_003761"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0578023.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6742..>7320)
                     /locus_tag="ACHUNN_003762"
     CDS             complement(6742..>7320)
                     /locus_tag="ACHUNN_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0578024.1"
                     /translation="IGRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDN
                     DGDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDN
                     VEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKE
                     SYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1909 a         1956 c         1798 g         1657 t
ORIGIN      
        1 ctttccacac tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc
       61 cttcctgaaa aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa
      121 ctatcatgaa ggtaaatggc gatgagtaaa gtgaaaagta tcacccgaga atcctggatc
      181 ctgagcactt tcccggagtg gggtagctgg ctgaatgaag aaattgaaca agagcaggtc
      241 gcccctggca catttgcgat gtggtggcta ggctgcaccg ggatctggtt gaaatcagaa
      301 ggtggcacca acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac
      361 ccgttaatga aacagggtca ccagatgcag cgcatggctg gcgtgaaaaa actgcagcca
      421 aacctacgca ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta
      481 ctggcgactc acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag
      541 aactgtgctg atgacgtacc attcattgga ccgaaaacct gcgttgattt gtggattggc
      601 tggggcgtac cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtgaaa
      661 gacattgaaa ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc
      721 gatcaaaaag cggctggcgt actgccagat ggcatggacg atcgcgcagt aaactacctg
      781 ttcaaaaccc ctggcggttc cctatatcac agtggcgatt cccactactc taactattat
      841 gcgaagcacg gtaacgaaca tcagatcgat gtggcgttag gatcgtatgg tgagaacccg
      901 cgcggtatta ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat
      961 gcgaaagtgg ttatcccgtt ccaccacgac atctggtcaa acttccaggc cgatccgcaa
     1021 gagatccgcg tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt
     1081 atctggcagg ttggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac
     1141 tatccgcgcg gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc
     1201 ctgtaatctg tcgtttatac cggatagcgt ggagaagttc gcgctatccg gcgtagtatt
     1261 tcctagctat ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg
     1321 aagcacaaag acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg
     1381 agaaagtcgt tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac
     1441 ttgacgaacg cggcaaactg aaaaaggtgc gcaatggcgc agaagctatt acccaacagc
     1501 gcccgcgctg gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta
     1561 ttgctaaagc ggcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct
     1621 ccaccgcgtt tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt
     1681 atctaccgct ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggtg
     1741 gacagtacaa caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct
     1801 atgccgggca ctggatgttt accagcggaa aagggctgac cgcagaaggg ttgtataaaa
     1861 ccgatatgct gacagcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg
     1921 tactggttga tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc
     1981 aaatcgatat gcttatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag
     2041 cgcaaggggt cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat
     2101 ctaacgcctc aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt
     2161 tatcccgtcc cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa
     2221 cgtcatcgtc gaggccatgc cacagcagta gaggtctgtc cccaagttgt tccaggtggt
     2281 ttgtcgcttc ccactctgcc agcggcgcga taatgttatt gaattcattc tgctgtgctg
     2341 ccgtttcagg tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc
     2401 ccatcatgct ggcggtacat ttcaccgtgg ggtggcgagc ggtaattccc agtgccgtca
     2461 tcgcgcccat cgacgcgcca ccgactgcca gacggtcatc aagcagccag ttttcttcgg
     2521 ctattgccgc acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt
     2581 gatttaaccg ccgcgcttcg tcgccactaa aacggctacc gtgatcgggc gcatccggca
     2641 tgatcacccg caaaccagcc tgcgccagcg caacggcaaa atagctatac accaggctgg
     2701 atgaagtaaa gccgtgataa aaaattacgc acggtaacgg ggtatctttt tgcccgacag
     2761 gataagcatg aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc
     2821 tctccttttt gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat
     2881 tagttcataa tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt
     2941 accaggtaac aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg
     3001 ttagcaggaa acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt
     3061 taagtgcctg tagcgcactg caaggtacgc cacagccagc accgccagta acggaccatc
     3121 cgcaagagat tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga
     3181 tggttcgggg ttctccggat gacgcattag cagaaatcag agcaaaagct gtcgctgcaa
     3241 aagctgatta ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt
     3301 cacaagccat tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct
     3361 ttgcgtccct atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta
     3421 ttaccgcgat ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa
     3481 caattacttg cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag
     3541 tctgcagaat tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca
     3601 gttagcaata tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat
     3661 gaacaaggtg cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac
     3721 tggcgcgtac aggcgatact gtatgcatga gtgtcatcta cagcaaatga acaatgccgg
     3781 atgcggcgcg agcgccttgt ccggcctaca attttcacat atttcattga gttaatcgaa
     3841 accagcgtcg catcagtcgt atacgtttac aaacacactc cccccgtcgc ccgcagcaat
     3901 aaatcattct ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga
     3961 caccacgcct gcgccattgg cggagaagca tatttcaaca tttgcgcacc gcagcccagc
     4021 aggaatagct gatgagtaat ctctcgcccc agttcttcag ctggcttacg cagctgctgc
     4081 tgtaaacgac gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaatgct
     4141 tccgacaata agtcgtatac gcccgcttgc ttattgagaa cgcgcaacac atccaggcac
     4201 ataatattgc cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc
     4261 agctcgctct cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac
     4321 ggcatacctc gtttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg
     4381 gcttctttgg catcggcgcg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt
     4441 tgcccttcaa gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc
     4501 aatggattac caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaacgcacgg
     4561 cgcatcatgg catggctacc cagggcgcaa tcaaaacgcg tcatcccccc cattttcagg
     4621 atcagacgaa ttccttcccc ttcctgcccc aataaccagc caatggcatc ctgaaactcc
     4681 acttcacaac tggcgttaga gcgattaccc agcttatctt tcagccgttc gaggcgaatc
     4741 gcgttgcgtt gcccgtcagg caaaaagcgc ggcacaaaaa aacaggacag accgcccgtg
     4801 gtctgcgcca gcaccagatg cgcatcgctt tgcggaaccg agaaaaacca tttatgcccc
     4861 accagccgat aagagccatc ttccaaacgc tctgcacggg tggtgttgct catgacatcg
     4921 gaaccaccct gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct
     4981 ggcaataagt gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc
     5041 gtgggtaaca tctgtaacaa caatggcgtg gcggcaaagg tcatcgttat tggacataac
     5101 gacccagcct caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg
     5161 gagcgagcgt cttcttccca ggcaagattg tgcacccgat tggtacataa cacctgcatc
     5221 agcaggtgcc atgcgggatg gaaacgaaca tcgtccagac gccgcccctg ggcatcgtag
     5281 cgcaacagtt caggcgggtt tacattcgcc agccgcccca gttcaaggga ttcagccgtt
     5341 cctaactgct gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt
     5401 accgcttcgc aaagcgcgcc atcagacagg tataagttgc tgttatttaa tggtataggt
     5461 tgattaaaaa cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac
     5521 cattagtatg gccactgcca tggattcatg acagaaagag attgctaaaa cattcttatc
     5581 cagcaatgta atgcggaacg aaacgtgagg tgtctttggt gatcagtgtg ttatcttcac
     5641 ggatccccat tccgcacgct tcatcatcga caatccagct atcgatgagt gtgtagctat
     5701 cgccaaatcg cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac
     5761 catcagcgtg gtcaacgacg ttattctgac cgtcgaaaat ggtgacgttt ccgccttcgc
     5821 gcgagtagat cggtttacgc acatagcttt cgccagcggc aatctgcgat ttttcaccct
     5881 caaaccagga tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta
     5941 gccctttatt actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc
     6001 acagcagcgg accgttatcg tcacgcatca tccactccag cggatacagc ttaaatgcac
     6061 gctggatgac attatcatca agatcggtca gtacaccgcc gacgcccaaa ccgagatctt
     6121 caatgtagat aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca
     6181 cggtagtcct gtcttcatcg gtgtcctgac agcagcagaa ataaaacggc tcccggctat
     6241 aaagctcgct aaagcgtgaa atcaggcgtt cctgaatggc attgtactga tcggcatcac
     6301 gcggaataat gccgctgcgc cgggcatcct ccagccacaa ccactggaaa taagccgact
     6361 catacaacga agtcggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac
     6421 accaggcaaa atccatccgg ccatacagcg aaggatcgct ggcgtgccag ctttcggcga
     6481 tcacatccca gtacaacggc gggatcgcca gttgcgtcag gatctcttcg tctttaaccg
     6541 cgcgatccac cacctcaagg cacatctgat gcagttccgc agttggtttt tcgatctgct
     6601 cttcaatctg gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt
     6661 cgatgatatg aaagtcgaaa ccgttgtcag cggcaatttg gtccagatcc cgccgcacag
     6721 gaacgttgtg tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc
     6781 atagccgccg cgcgaaacgg ttgacgcttt tttggtggta aagccgcctg aagagtaaga
     6841 ctcttttttg ccagaaccgg agcgccagga gtaatcaccg gaagtgttgc gccagacggg
     6901 gcgggaagca aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg
     6961 aataacccgg cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc
     7021 gtagtaggaa ttttcgtact tagactgaca gttctgctga gtcatattct tcggaacatc
     7081 ggcataaaat gccgttttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc
     7141 gtcatcaata caatcctgca ctgtcgcgta aaacgttccg tcgccgtcgt tatcgacatc
     7201 gctactatcg ccacaacctt tcaatacgaa aaaagcggca ccgcccatta gcgccaacgt
     7261 taagtatttt tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaat
//
LOCUS       DAZOXR010000202         7239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZOXR010000202 DAZOXR010000000
VERSION     DAZOXR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.209"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..337
                     /locus_tag="ACHUNN_003763"
     CDS             <1..337
                     /locus_tag="ACHUNN_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0578025.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="ACHUNN_003764"
     CDS             337..666
                     /locus_tag="ACHUNN_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0578026.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="ACHUNN_003765"
     CDS             676..1374
                     /locus_tag="ACHUNN_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0578027.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="ACHUNN_003766"
     CDS             1379..2122
                     /locus_tag="ACHUNN_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0578028.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="ACHUNN_003767"
     CDS             2059..2661
                     /locus_tag="ACHUNN_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0578029.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="ACHUNN_003768"
     CDS             2722..6201
                     /locus_tag="ACHUNN_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0578030.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="ACHUNN_003769"
     CDS             6269..6868
                     /locus_tag="ACHUNN_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0578031.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="ACHUNN_003770"
     CDS             6932..>7239
                     /locus_tag="ACHUNN_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0578032.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgc
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAZOXR010000203         7154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZOXR010000203 DAZOXR010000000
VERSION     DAZOXR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.210"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..4519)
                     /locus_tag="ACHUNN_003771"
                     /pseudo
     CDS             complement(1..4519)
                     /locus_tag="ACHUNN_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4539..5000)
                     /locus_tag="ACHUNN_003772"
     CDS             complement(4539..5000)
                     /locus_tag="ACHUNN_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR0578033.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5028..6929)
                     /locus_tag="ACHUNN_003773"
     CDS             complement(5028..6929)
                     /locus_tag="ACHUNN_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0578034.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1324 a         2191 c         1949 g         1690 t
ORIGIN      
        1 tttatttgac tacgtgataa acgaacccca tccaggaaac ccgtatgccc actaacacga
       61 cgaccatcgc caatatagtc catcaaactg ctgattttag aacttgcagc caatcccagc
      121 ggatcaaccc acccacaagg attcggcaca taccggtaag tctgctcccc gccctgcaac
      181 cccagcggat cactcaccag gtacattccg gtttccggct cgtaataccg gaaccgatta
      241 tagcacagcc cggattccgc atcctgccac tgtcctgcat acagcaggcc ggggtcggct
      301 tccgggtcca gttccccgcg cgggtccggg tagccggtgt ctgcgggcag gctgagtgcc
      361 agcccccaca ggctggtctg ccccggtcgc cagaccgtct ttccttcact gttaaagagc
      421 atcagcgggc ggcccgtcag gtcgctcacc gcatgattcg tccgcgtcac ccactgcggg
      481 gccaccgacg gatgcgcctg ccgtacccgg ctgaactgct ggcttatcag ctcaaagccg
      541 ttaaacacca ggtgccttac gctgtacagt ttatcatcgc ggtattcccg gatttccgca
      601 atactgtcgc cgtcccacag atacgtgaag cggattttct tccggtcgca gcgtttctct
      661 gttcgtctgc cgctggcgtc gtggcggtat tcccacagct caccctgtgc gctgcaatac
      721 ccggtcagct ggtcccggct gtcccaccgg aaccgctctg tttccggtcg gtagccgtca
      781 cggtgtctgg tgcggctgac catccggcct gcggcgtcat agtcatactg cgtgtttccg
      841 gcctgtttca gccggtggcc tccggcatac tggtcagtct cttcacttat ccggtgcctg
      901 cccgcagact gcgccttcag gtagccgcag ctgtcatagc cataggactc atcgccttcg
      961 ccatagccca gcccggtgcc tgaggctgtc accgccgtga cctggccgtt ccggtccagc
     1021 cgatactccc gccgtgtttc ccgtccgtaa tcttcccggc tggtgacgcc tgacacgtta
     1081 cccgcgctgt cgtaacggta ctcccgtacc agtgagggaa ccagcccgtc ccatgtggct
     1141 tctgccggaa aaaatccggc gcgctgactg acaggctgac cggctgcatt ccagccctgt
     1201 tcacaggcaa aaccccggtt actgtgccgg agcacttcac gtcccgcctc atcacggctg
     1261 aacgtcagcg tctcatcccc cggcagaatg acctctgtca gttctccggc ggcgttgtag
     1321 cggtacgtgg ttttcagcag ggattcaccg gggtgtctgt cgcgttcatc ttccggtggc
     1381 aggatacagc ggatgacggt gtgttcatcg gggtagttgt attcaatggc ccgtcggccc
     1441 tgagtggtga cattcacccg cccgcgttcg tcatagccgg tgcgctgtga tgtgccgtcc
     1501 gggctgaaga tacccgtcag cctgccggat ttgtcatagg catactgcgt tttgccttcc
     1561 ggcgagatga tttctgccag gcggcctgcg gcgtcccggc tgtagaggat ggtttcctcc
     1621 aggccgttaa ccttggcggt acagaggcca tcggcgtcat agcggtaacg ccagaccacg
     1681 ccgccccagt cacgctcttc cgtcacccgg ccgcagttat ccagaacata aagatatgac
     1741 tcaccctgcg ggttgatgac ttctgtcagt tgcagcgtgt ctttatcgta gcggtactgc
     1801 cagcggtggc cttcaccgtc cgttcgcgcc accggaaaat cgaacggacc gtattccatc
     1861 gtccagacaa caccgttgcc gtccgtccag gccagcaggt tgccgtggcg gtcatagcga
     1921 aatccttccg cgccggttcc agcacgttca atgccgctca gtttatggtg ctgctgccac
     1981 tgtaaccgca ctgactgacc gccggggccg cgcatcttca ctggcaggtc gtgaccgctg
     2041 tattcggtgt ggtgttcacg gcccatcacg tcggtgagtg tttccggccg gttgtggtgg
     2101 tcgtactcta tgcccagacg ccgttcatcc tggcggaata ttcccgacag cagcccctga
     2161 gcggtgtatg ccagccgggt ctcttcgccc tgtggcgaca ccagacttac cggcaggccg
     2221 cgttcatcac actgctgtgt ccacgtctgc ccggcagggt cggtgatgtt gctgagcagg
     2281 ccgcgctcat catagcggta ctgccactcc gcgccgtctg ccgccgtgta acggcttaac
     2341 tgaccgtgtg cggcgttgta ttcaaatttc tccacccgcc cggcagggga gtggcgggcc
     2401 agcaggtgat ggaattcatc ccattcatac atggtggtgc tgccgtccgg cgcttcttcc
     2461 cgcagaatat tgccgtcagg atcatagtaa taacggtgaa tgcccttgcc gtccgtcacg
     2521 gtggtgcagc cgtcaccata gtccagcgtg gcgttgtaat agccctccgc cccggtgaca
     2581 ttcacgcacc ggccctgttc atcgtaggtg aaacggctcc acgtctggtc gttatcggac
     2641 cagcggatga tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca
     2701 tccgcttctg tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgtcg
     2761 ttatccgtgc ggtgaatggc tttcaggtag ccgctggcgt ggtacgtcag tttaagccgg
     2821 atcccgtcgg tgtgactgac acccgtgatc cggtactggc tgtcgcgctc aaacgtgatg
     2881 cggttgctgg cggtgtcgct catcccgccc agcatgtaca tccggtcatt ggggctgtgt
     2941 gccaggcggt catcgctggt atcgtcagtc accgggtggc gcagacgcat tcccggaaag
     3001 gcgtcataaa agaagcggct gctcagtgca tccctgtcga acagctccag ataatcatcg
     3061 cggcggcgca gaatgtactg ggcgtgatac gggttaaccg ccgtatcact gtcaccgtcg
     3121 aaggcaaatc tcagcgtggc tccctggctg atgatatcca cccggtttcc ctgttcacgc
     3181 acccaggcgt attcgctcca ggagtcgctc cagcccacgc cggtcaggcc atgcagcggc
     3241 agtacagaac tgtggaaacg ggtgaaggag agggggaggg tctggcccag ggtgaagtcg
     3301 gtacgttcgt cgcagtatgc gccggttacc ggatcgacag gatctgttac ccatctgatg
     3361 actttattgg caatactttt taacgactta cctgttttcc ccagcaacgc gccgagcttt
     3421 gcgcccagca gcttcccgct tttccccagc agtttcccag tttgccgagt ctcgccagat
     3481 tcttcagttt tcccagtagt cccgctggcg gcagaagata gctggctatc gttattcccc
     3541 ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatat gtgacctgct
     3601 caccgccaat aaagacgtcc ggcgaacctc ccacgatcgc cgcactgcac tccagtttgt
     3661 cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc tgggctatcc
     3721 tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca
     3781 catctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac
     3841 cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgctgt
     3901 ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg
     3961 ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggacttcag
     4021 gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg gccagctcgc
     4081 cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt
     4141 ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg
     4201 catacatcag cgcaggtacc agaaccagcg cataagggcc ggtggcacag gcggccataa
     4261 tcaggcaacc ttctgcgatg gtcactatcg cccccagtac ggcccctgcc agcgccccca
     4321 aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggcca gacttttttg
     4381 atgccaacat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca
     4441 tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc
     4501 cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg
     4561 atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt
     4621 tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc
     4681 cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc
     4741 caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt
     4801 tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt
     4861 gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca gattgatgcc actgttatct
     4921 gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct
     4981 tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa
     5041 acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg
     5101 acctgtagct gaatgctatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa
     5161 atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg
     5221 tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc agataaagac
     5281 cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc
     5341 tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg
     5401 gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct
     5461 ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag
     5521 cggatggtca tctgcgtctt tgttcccggc aggctgccgg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg ttattcagga
     7141 caggtacgca tatc
//
LOCUS       DAZOXR010000204         6883 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZOXR010000204 DAZOXR010000000
VERSION     DAZOXR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.211"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..372)
                     /gene="rnpB"
                     /locus_tag="ACHUNN_003774"
     ncRNA           complement(<1..372)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUNN_003774"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(405..1550)
                     /gene="garK"
                     /locus_tag="ACHUNN_003775"
     CDS             complement(405..1550)
                     /gene="garK"
                     /locus_tag="ACHUNN_003775"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0578035.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1647..2537)
                     /gene="garR"
                     /locus_tag="ACHUNN_003776"
     CDS             complement(1647..2537)
                     /gene="garR"
                     /locus_tag="ACHUNN_003776"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0578036.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2567..3337)
                     /gene="garL"
                     /locus_tag="ACHUNN_003777"
     CDS             complement(2567..3337)
                     /gene="garL"
                     /locus_tag="ACHUNN_003777"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR0578037.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3353..4687)
                     /gene="garP"
                     /locus_tag="ACHUNN_003778"
     CDS             complement(3353..4687)
                     /gene="garP"
                     /locus_tag="ACHUNN_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0578038.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5062..6633
                     /gene="garD"
                     /locus_tag="ACHUNN_003779"
     CDS             5062..6633
                     /gene="garD"
                     /locus_tag="ACHUNN_003779"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0578039.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6782..>6883
                     /gene="prlF"
                     /locus_tag="ACHUNN_003780"
     CDS             6782..>6883
                     /gene="prlF"
                     /locus_tag="ACHUNN_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR0578040.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
BASE COUNT         1690 a         1888 c         1694 g         1611 t
ORIGIN      
        1 aaactgaccg ataagccggg ttctgtcgtg gacagtcatt catctaggcc agcaatcgct
       61 cactggctca agcagcctac ccgggttcag tacgggccgt accttatgaa cccctatttg
      121 gccttgctcc gggtggagtt taccgtgcca cggactgtta ccagccgcgc ggtgcgctct
      181 taccgcaccc tttcaccctt acctgatccc gcttgcgcgg gccatcggcg gtttgctctc
      241 tgttgcactg gtcgtgggtt tcccccccag gcgttacctg gcaccctgcc ctatggagcc
      301 cggactttcc tcccctccgc ccgtctcccc cgaagaggac gacgacgaag cggcgactgt
      361 ctggtcagct tcggcgcgca gtatagaggg tttgcgcgcc cttgtcaccc cgcgttgcgc
      421 attccaatcg ccagtgtcgc ggcgatatta cgtgaagcac ggcagatatt gtcataagcc
      481 ccgcggaatg cttcgtccaa cgtacctatg ctggtcaata cgctgaagac cgcatcgatg
      541 ccatgctgat gtacaacgcc aacatcatcg gtcaggctac ccgcaatgcc aatcaccggt
      601 ttatggtact tcttcgccac gtttgcaacg ccaatcggta ccttgccgtg aatactctgg
      661 ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat cgtgaatatg ttcttccaga
      721 ttcagcgccg tagtgacgat ttcaatacca cttttcagtt ccgcaccaag aaacgccatc
      781 agcgccgcgc ccataccgcc cgcagctcct gcgccgggga catctttcac atcaacatgc
      841 agcgcttttt taatgacatc ggcatagtga gagaggttat tgtccagctc aacaatcatc
      901 gcttcactgg ctcccttttg tgggccaaag atgcgcgatg cgccgttatc gcccaccagc
      961 ggattggtga catcacaagc gacgcgaatg acgcaatctt ttaagcgcgg atcgaggccg
     1021 gaaatatcaa tatcattcag agtattaaga ctaccgccgc caaaaccaat ttcattgccg
     1081 ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc ctgcgccgcc atcatttgta
     1141 gcgctgccgc caatgccgat aataatgttt gttgcactgc tctccagcgc ctgcaggatt
     1201 aactcgcctg tgccgcgtga agtggttacg agtggatcgc gtttttccgc aggtaccagc
     1261 tccagcccac tggccgccgc catttcaata aacgcggttt tgccatcgcc ggagatcccc
     1321 caactggcat tcactttctc gcccagcggc cctgtaaccc aggcgtgacg ttcagccccc
     1381 tgggtggctg caatcatcgc ttccaccgtt ccttcaccac cgtcggcaac cggaacagaa
     1441 acatactgtg catcaggaaa aatttcccga aatccttttt ctatcgcctg cgcaacctcg
     1501 ctggcagata aactttcttt ataagagtct ggggcgatta cgattttcat acctatgcct
     1561 gttaccacat gacgccggag ggcgtttctc ttattcggcc tagattccag gcccggattg
     1621 caatacacca tccgggcacg acgtcattaa cgagtaactt cgactttcgc cagtttttcg
     1681 tagtagcacg ccagggcgct atgatccgcc gttcctaaac catctgcacg cagtgcctgc
     1741 atcatctcca taaccgcagc tgtgagcagc agttgtgcgc cgacgccgtg agaagtatcc
     1801 agcgcattcg ccagatcctt aatatgcaga tcaatgcgga agcccggctt gaagttgcgg
     1861 tccatcacca tcggcgcttt ggcatccagc acggtactgc ccgccagccc accgcgaatt
     1921 gcctgataaa ccaggtccgg gttaacgccc gctttggttg ccagcgttaa cgcttctgac
     1981 atcgcagcaa tattcagcgc cacaatgacc tgatttgcca gtttggtgac gttacctgca
     2041 ccgatttccc cggtatgcac cacggaaccc gccatcgctt tcatcaaatc atagtatttg
     2101 tcgaaaatag ccttgtcgcc gcccaccatc actgacagcg taccgtcgat ggctttcggt
     2161 tcaccgccgc tcaccggagc atccagcata tcaatgcctt tcgctttcag cgcttcgctg
     2221 atttcacggc ttgccagcgg tgcgatagaa ctcatatcga tcaataccgt acctggcttc
     2281 gcgccttcaa taatgccatt ctcacccagc gccacctctt tcacatgagg ggagtttggc
     2341 agcatggtta tgatgacgtc gcactgttca gcgatcgctt tagccgtaga cgctgtttct
     2401 gcacctgcag caatcacgtc agcaatagct tctgggttac ggtcagcaac caccagcgag
     2461 taacctgctt tcagaaggtt tttactcatt ggtttaccca taatccccag gccaataaaa
     2521 ccaactttca tagtcatatc aaccatctct cttgttgcgg tggtggttat tttttaaagg
     2581 tatcagccag tttctgagtg gcagagcgga agacgccaag atcgctgccg acagccacaa
     2641 acgtcgcgcc ccattccaga taacgacgcg catcggcttc gaccggcgcg agaataccgc
     2701 tgggtttgcc gtgcgcgctg gcacggttaa aaatatgctg aattgctttt tgtacatccg
     2761 gatgcgaggc attgccgaga tggcccaatg ccgcagccag atcgctgggg ccgacgaaga
     2821 taccgtctac accttcggta gcggcaatag catcgacgtt atctacgccc tgctgacttt
     2881 ctatctggac cagaatagta atgttcttgt tcgactgagc gaaataatcc gccacggtgc
     2941 caaacatatt ggtgcggtga gaaacggaga cgccgcgaat gccttccggc gggtaacgag
     3001 ttgatgccac cgcctgctct gcttcctctt ttgtttctac aaaaggaatc aggaagttat
     3061 agaaaccgat atccagaaga cgcttaataa ttaccggctc gttggtcggc actcgcacta
     3121 ctggcgcgct ggcgctgcct ttcagggcca ttaactgcgg aataaacgtg gaaatatcgt
     3181 ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc agccaaacca agaacttcag
     3241 tgctaatcgg gttagagagt gctgaccagc aaccaatttg tacctgtttc gcagccagtg
     3301 cggctttgaa tttattcggg aaaacatcgt tattcatcgc ttataccttt gcttatttct
     3361 gcaattccat acgtttaatg tcgccaacta cgaagaggta gcagaccatc gccatcagcg
     3421 ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg cagttcactt accaggtagc
     3481 caatcaccag tggagtgaca atggaggcaa cattgccaaa gacgttaaag acgccgccac
     3541 agaggccaac aatctctttc ggcgcggtgt cagaaatcac cggccagccc agagcaccaa
     3601 atcctttgcc aaaaaaagcc agcgccatca gcatgaccac cagcgtggtg ttgttggtgt
     3661 agttacataa gatgatggtg gaaaccagca acattcccag cacaatcggt agcttacgtg
     3721 ccagggtcag ggataaaccg cgtttgatca gataatccga gaagacacct cccagcacgc
     3781 cgcccgcaaa accacacagt gccggaatcg aggcgaccag acccactttc agaatcgaca
     3841 tgcctttttc ctgcaccaga taaatcggga accaggtgag gaagaaccag gtgatggtgt
     3901 tgataaaata ttgtccgaaa aatacgccca acatcatgcg gttagagagc aattgcttga
     3961 tgtaatgcag tttgggaccg cttgctgccg cgctgcccgg ctttttgtgg tccatatcga
     4021 ccaccgcgcc attttcagag ataaacttca gctcttccgc agacatacgt gggtgatctg
     4081 tcgggttatg aatcaacttg atccacagcg ccgtcagcac aaaaccaatc ccccccataa
     4141 cggtgaagac gtgctcccag ccccaagcga aagtcagcca gccaagcagc ggcgaaaaga
     4201 gcgccagcga gaaatattgc gccgagttaa agatggcgga ggcagtacca cgttctttcg
     4261 tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa tgatggcgct tccgagaacc
     4321 ccagcataaa gcgcataaag aacatggaga tccctgccca ggccagcggg aacatatcaa
     4381 caaagccttg caggaaggtg aacagcgacc agaaaaagag gctgtaggtg taaacttttt
     4441 tcgagccaaa cttatcaagc aaccagccgc cggggatttg catcagcaag taggcccagc
     4501 caaaagcaga gaagatgtaa cccatcgaaa ccgcacttaa ctgcaactct tttgccactt
     4561 cggtaccagc aatagacagc gttgcacgat cggcgtagtt aacggcggta acaataaaaa
     4621 taatcagtaa tattaaatag ctggtatgca cgcctttctt ttttacgtca acggtgtcca
     4681 gaatcatttt atttacctcg ggtacttatg ctgattttta ttattatggg gaaggtgtta
     4741 tttatgagtt ttatttatgc cgttacgaca atgaactcgg gaattagtat aagcagcggg
     4801 agaataataa tcattgtgca aatgctaatt taatcagtac tatttaaata ttattttgag
     4861 catatgcaca taaggttgcg cgctaaagca cagatttgcg ctttacctta ccgggcggca
     4921 ctgcaatccc tgaaatgatt gacattgatc acatttctgc gtttaaactc ctgacattct
     4981 tatttcaccc aatgaagtca tttattttta aatgagacca gatcctcatt ttaataaccc
     5041 ctggctggag aatattgcac aatggccaac atcgaaatca gacaagaaac gccaactgcg
     5101 ttttatataa aagttcacga cacagataat gtggcaatta ttgttaatga taatggcctg
     5161 aaagcaggaa cgcgttttcc ggatgggctg gaattaattg aacatactcc ccaggggcat
     5221 aaagtcgcat tgctggacat tccagctaat ggtgaaatta ttcgttatgg cgaagtgatt
     5281 ggttacgccg tgcgcgcaat cccacgcgga agctggatcg acgaatcaat ggttgtacta
     5341 ccggaagcgc cgccgttaca cacgctgcca ctggcaacca aagtcccggc ccccttaccg
     5401 ccgctggaag gatacacctt tgagggctat cgcaatgccg atggcagcgt gggcaccaaa
     5461 aacctgctcg gcatcaccac cagcgtccac tgtgtggcag gcgtggtgga ctatgtagta
     5521 aaaatcattg aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt ggtggggctg
     5581 aatcatttgt acggctgtgg cgtggcgatt aacgcaccgg cggcagttgt gcctattcgt
     5641 accatccaca atatttcgct gaaccctaac tttggcggcg aagtaatggt gattggtctg
     5701 ggttgtgaaa agttgcagcc tgagcgcctg ctggtcggca cggatgatgt gcaagctatt
     5761 ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg ctttcagtcc
     5821 atggtcgagg atattttgca ggtcgccgaa cgccatctac aaaaactgaa tcaacggcag
     5881 cgagaaacct gcccggcttc agaactggtc gttggtgtgc agtgcggtgg cagcgatgcg
     5941 ttttctggtg taacggcaaa cccggcggtt ggctatgcgt ctgatctact ggtgcgctgc
     6001 ggcgcaacgg tgatgttttc agaagtaacg gaagtgcgtg acgcgatcca tctgctgaca
     6061 ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga gtggtacgat
     6121 aactatctca atatgggaaa aaccgaccgc agcgccaacc cttcgccggg caacaagaaa
     6181 ggcggtctgg caaatgtggt ggagaaggca ctcggctcca ttgctaaatc gggtaaaagt
     6241 gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta aacgcggatt aatttacgcc
     6301 gcgacgccag ccagcgattt tgtctgtggc acgcaacagg tggcttcggg tatcaccgta
     6361 caagtgttta cgaccggccg tggtacgccg tacggcctga tggcggtacc cgtaattaaa
     6421 atggcaaccc gcaccgagct ggcgaaccgc tggtttgatt taatggatat taacgcgggc
     6481 actatcgcta ccggcgaaga gagcattgaa gaggtgggct ggaagttgtt ccactttatt
     6541 ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc aatgggggct gcataaccag
     6601 ctggcggtgt ttaacccggc accggtgacc tgatttcttt tggaacgtcc ctcgcaaaac
     6661 atggccttag tgccatgttt ttattgttta aagcccccac gtccattaat aatgcatttg
     6721 cattacctta gttcaagctt ataatttgag cagaaaacag gctgtaaaag gacaatgaat
     6781 catgcccgct aatgctcgct ctcacgctgt actgaccact gaatcaaagg tcacgatacg
     6841 cggacaaaca actatccccg cgccagtgcg tgaggcctta aaa
//
LOCUS       DAZOXR010000205         6789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZOXR010000205 DAZOXR010000000
VERSION     DAZOXR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.212"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..2635
                     /gene="cas3"
                     /locus_tag="ACHUNN_003781"
     CDS             <1..2635
                     /gene="cas3"
                     /locus_tag="ACHUNN_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR0578041.1"
                     /translation="KTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQ
                     LGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGY
                     WLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGWPPDRMDELDNFLPEDKA
                     AARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRV
                     AHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDI
                     SQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKT
                     WLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSN
                     KKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLI
                     ERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDV
                     NSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLL
                     ARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLICTQVLEQSVDC
                     DLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPG
                     DEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGF
                     ARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCI
                     DGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVI
                     LVNDDESCGYSATEGLIGKVG"
     gene            2733..4294
                     /gene="casA"
                     /locus_tag="ACHUNN_003782"
                     /gene_synonym="cse1"
                     /pseudo
     CDS             2733..4294
                     /gene="casA"
                     /locus_tag="ACHUNN_003782"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            4291..4827
                     /gene="casB"
                     /locus_tag="ACHUNN_003783"
                     /gene_synonym="cse2"
     CDS             4291..4827
                     /gene="casB"
                     /locus_tag="ACHUNN_003783"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR0578042.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4839..5894
                     /gene="cas7e"
                     /locus_tag="ACHUNN_003784"
     CDS             4839..5894
                     /gene="cas7e"
                     /locus_tag="ACHUNN_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR0578043.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5905..6651
                     /gene="cas5e"
                     /locus_tag="ACHUNN_003785"
     CDS             5905..6651
                     /gene="cas5e"
                     /locus_tag="ACHUNN_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR0578044.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6576..>6789
                     /gene="cas6e"
                     /locus_tag="ACHUNN_003786"
     CDS             6576..>6789
                     /gene="cas6e"
                     /locus_tag="ACHUNN_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0578045.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
BASE COUNT         1688 a         1616 c         1850 g         1635 t
ORIGIN      
        1 aaaaacccga cgcggcgaga aagacggggg cgacgactat caccttcttt gctggcattc
       61 gctggatgta gccgcgatgg gctatttaat ggttaaaaga aattgcttcg ggctggctga
      121 ttattttcgt caattaggga tttctgacaa ggaacaggcg gctcaatttt tcgcctggtt
      181 gctgtgctgg cacgatattg gaaaatttgc ccgctctttt caacaacttt acctgccccc
      241 tgaactcaag attcaggaag gtgccagaaa aaattacgaa aaaatctctc attcaacgct
      301 gggctactgg ttgtggaatc attatttaag tgaatgtcag gagttacttc cttcatcttc
      361 gctctctccg cgcaaactta ggcgagtgat agaaatgtgg atgcccgtga cgacagggca
      421 tcatggttgg ccccctgatc gtatggatga attggataat tttctgcctg aagacaaagc
      481 tgcagcacga gattttctcc ttgaaatcaa accactgttt ccgctcatag agatccccgc
      541 attctgggat gatgatgagg gcattgaact tataaaacac ctttcctggt atatctctgc
      601 aaccgtcgtg ctcgcagact ggacgggttc gtcaacgcga ttttttccac gcgtcgcaca
      661 cccaatggat attaaaggtt actggcagaa aactttagtt caggctcaaa acgccttaac
      721 cgtctttcct ctaaaagcaa aagtcgcgcc tttcaacgga attaatacgc tgtttccttt
      781 tattgagaat ccgacaccgt tacagcaaaa ggtgctggat ctggatatca gccagcaagg
      841 accgcagtta tttattctgg aagatgtgac tggcgcaggt aaaacagaag cggcgcttat
      901 cctggcgcac aggttgatag ctgcggggaa ggctcagggg ctgttttttg gtctgccaac
      961 aatggcaacg gccaatgcca tgtacgatcg gctggtcaaa acctggcttg ctttctattc
     1021 gccagaatcc cgccccagcc tggtgctggc acatagtgcc cgcacattaa tggaccgctt
     1081 taatgaatca ctctggtctg gtgatttagt cgggtcagaa gaaccggatg aacaaacgtt
     1141 cagtcaggga tgtgcggcct ggtttgccga cagtaacaag aaggcgttgc tggctgaaat
     1201 tggcgtcggc acgctggatc aggcgatgat ggcggtgatg ccgttcaaac ataataatct
     1261 gcggcttctg gggctgagta acaaaatcct gctggctgat gagatccacg cctgtgacgc
     1321 ttacatgtcg tgcattcttg aagggttgat cgagcggcag gcgcgtggcg gaaacagcgt
     1381 tattttgctt tctgccacgt tatctcaaca gcagcgcgac aaactcgtcg ctgcctttgc
     1441 gcggggtaca gagggcctgc aagaagctcc gttcctggaa aaggatgatt acccctggct
     1501 gacgcatgtc acgaaatccg atgtgaactc acaccgggta gcgacgcgca aagacgttga
     1561 gcgtagcgtc agcgtgggtt ggcttcatag tgaacaagag tgtattgcgc gtatcgaatc
     1621 ggcggtaagt caggggaaat gcatcgcctg gatccggaat tctgtcgatg acgctattaa
     1681 ggttcatcgt cagctgcttg cccgcggcgt cattcctgct tccagccttt cactttttca
     1741 tagccgcttt gcttttagcg atcgccagcg aattgaaacg gagacactgg cacgctttgg
     1801 taaagaagat agctcacagc gtgccggaaa agtccttatt tgtactcagg tattagagca
     1861 gagtgtcgat tgtgacctgg acgaaatgat ctccgacctg gcccctgttg atttgctgat
     1921 tcagcgagca ggacgattac agcggcatat ccgcgatatt aatggtcagt taaagcgtga
     1981 cggaaaggac gagcgttccc cccctgaatt gctgattctg gctcccgtct gggacgactc
     2041 tcctggcgac gaatggtttg gcagtgccat gcgtaacagt gcatttgtct atcccgatca
     2101 tgggcgaatc tggctgacgc agcgtgtgct gcgtgagcaa ggtgctattc agatgccaca
     2161 cgcagcccgg cttcttattg aatccgtcta cggtgaggac gtggtaatgc cggaaggatt
     2221 tgcccgcagc gagcaggagc aagtgggcaa atattactgt gatcgcgcaa tggctaaaaa
     2281 gtttgtgctg aacttcaggc ccggctatgc cgccaatatc aacgattacc tcccagaaaa
     2341 actgtcgaca cgtctggctg aggagtctgt ttccctgtgg ctggctacct gtattgacgg
     2401 tgtggtgaag ccttatgcca ccggtgctca cgcatgggaa atgagcgttg tcagagtgcg
     2461 ccgaagctgg tggaaaaaac atcgggatga gttttcttta ctggagggag aggcgttcag
     2521 gctgtggtgc attgaacagc gacaagatcc ggagatggca aacgttattt tagtcaacga
     2581 tgacgaaagt tgtgggtatt cggctacgga aggattgatt ggcaaggttg gttgagatgt
     2641 ctgcgacagg actctctgaa tagttgatac accgaaaagg atggaaagat ggacaaccga
     2701 cctgggatgg tcttttttac gagaggatgc gtatgaactc gttttcactt ctgacaaccc
     2761 cgtggttgcc cgttcgtttt aaagacggaa cgacaggcaa actggcgcca gtcgatctgg
     2821 cggatgaaaa tgttgtcgat atttctgcgc cgcgggcaga tctccagggt gcggtatggc
     2881 agtttttgct ggggctacta caaaccagct tcgcgccaaa agatcatcgt cgttgggatg
     2941 atatctggga agacggactg gaagctgaaa agctacggga agcattgctg tcattagaac
     3001 atgctttcca gtttgggcca gattcccctt catttataca ggatttcgag gcgctcaccg
     3061 gagataaagt tccggtcgct tcgctactgc ctgagattcc cggcgctcaa acaacgaagt
     3121 ttaataaaga ccactttatc aagcgtggtg taacggaata cctgtgccca cattgctctg
     3181 cgttagctct gttctcccta cagttgaatg cgccgtcagg tggcaaaggc taccgcaccg
     3241 gtttacgcgg cggtgggccg atgaccactc tgattgaatt acaggaatat cagggtaatc
     3301 aacaaactcc cttgtggcgc aaactgtgga tcaatgtcat gccgcaggat gaagccgatt
     3361 taccgctacc caaaaaattt gacgatctgg ttttcccctg gcttggcccg acgcgcatca
     3421 gcgaactggc cggtgcggtg gtaaccgatg agcaggtcaa taaactccag gcgtactggg
     3481 gaatgcctcg ccgtattcgt attgatttta ataccaccac agtcggcaac tgcgatattt
     3541 gcggtgagca gagcgacgcg cttctgagcc tgatgacgac caaaaattac ggcgctaact
     3601 atgccatgtg gcagcatccc ttaacgcctt accgtgtact acttaaagag ggcggcgagt
     3661 tttactcggt caaaccacaa ccgggcggtt taatctggcg cgactggtta ggccttatcg
     3721 aaacgggtaa gtcagaaaac aatacggaac ttcccgcgct ggtggtgaaa ctctttaatg
     3781 ccagcagtct caagcaggca aaagtgggcc tgtggggatt tggctatgat ttcgacaaca
     3841 tgaaagcgcg ctgttggtac gaacatcatt tcccgctgct gctcaataaa aagaaggcca
     3901 gataccgaaa ctgcggctgg ctgcgcaaac ggcttcacgg attctaagtc tgttacggag
     3961 tgcattgaaa gaagcatggt tctccgatcc aaaaggtgca aggggtgatt ttagttttgt
     4021 ggatatcgac ttctggaaca aaactcagca tcgcttcctg aggttagtgc gccaaattga
     4081 agaaggtcag gatgcggatg aattactcag caaatggaat aaggaaattt ggttattcgc
     4141 gcgtcaggat tttgacgagc gtgtattcac caatccttat gagcccgttg atttggaacg
     4201 catcatgacc gcgcgcaaga aatattttac aacatcggcg gagaagcaaa gtgctaaagc
     4261 cgccagggag aaaaagcagg aggctgctga atgagtattg taaaagagga gcacaaagcc
     4321 acgctgcgca aatggcacga ggaattgcag gagaaacgcg gcgaacgtgc cagtctgcgg
     4381 cgtagtacga cggttaatga tgtttgtctg acggatggat ttcgcctgtt tctgaagaac
     4441 agacaaataa agtggcagga tgaacctgag tggcgtatca ccgcactggc gctgatagcc
     4501 gcagttagcg ccaatgttaa agccattgat gaacggcagc cttttgccgc gcaactggcg
     4561 gcggtaatgt caaaggggcg ttttactcgc ctgtcagcag taaaaacacc ggatgaatta
     4621 ctgcgccagt tacgccgcgc cgtcagatta ttaaacggtt ccgtcaatct tgactccctg
     4681 gcagatggca ttttccgctg gtgccaggag agcgacgacc tgctcaatca tcaccgccgc
     4741 cagcagcggc caaccgaatt tatccgtatc cgctgggcgc tggaatatta ccaggctggc
     4801 gacgccgata acgaacaaaa tcaataaagg actatctcat gacaacattt attcagcttc
     4861 atgtgttaac cgcttaccct gctgccaacc ttaaccgtga tgacaccggt gcgccaaaaa
     4921 ctgtggtgct gggtggagca acgcgtctgc gcgtttcctc gcaaagtctg aaacgtgcgt
     4981 ggcgcacttc tgcacttttt gaacaggcac tggcgggcca tattggtatt cgcagtgggc
     5041 gtattgcgcg tgaggcggca actatcctga ttgagaaagg gattgaagat aaaaaagcca
     5101 tcgaatgggc ggcaaaaatt gctgattatc ttgggaaagc taaaaatgac aaaaaaccaa
     5161 aagatccgct cactaacgcc gaaactgaac aattagttca tatcagcccg gcagaatttg
     5221 acgccgtaaa agcgcttgct catcaactgg cagaagaaaa gcgcgcgcca aaagaggaag
     5281 atctcgcgtt gttacgtaaa gaccgtatag cggtagatat cgctatgttt ggtcgtatgc
     5341 tggcgaataa acctgagttt aatgttgaag ccgcctgcca ggtcgcgcat gcgtttggtg
     5401 tcagtgaaac gattgtcgaa gatgattttt tcaccgccgt tgatgatttg cgccaggcct
     5461 ctgaagatgc cggtgccggg catctcggag aaaccggatt cggttcagca cttttctaca
     5521 cctatatctg cattgataaa gatctgctgg tagaaaacct cggcggtgac gaagcgttag
     5581 ctaatcagac catacgcgct tttacggaag ccgcgcttaa agtttcccca accggtaaac
     5641 aaaatagctt tgccagccgt gcctatgctt cctgggcgct ggcagaaaaa ggcaccgatc
     5701 aaccacgctc tctggcggcg gctttctatg aacccattaa tggcacacgt cagttagatg
     5761 tggcggtgca gcgtattaca acgcttcgcg aaaacatgaa tacggtctac gaacagaaga
     5821 ccgaatgcgc aagttttgac gtgatgaaca aacagggaag catgaaggac gtgctggact
     5881 ttatctgcgc gtaaggggcg gcttatgagc caatatctga tttttcagct tcatgggccg
     5941 atggcgtcct ggggtgtcga tgctccaggt gaagttcgtc atacccatga acttccttcg
     6001 cgctcagcat tgctggggct actggctgcc ggggtaggga ttcggcgtga tgataccgaa
     6061 cgcttaaacg cgtttaatcg ccactattca ctggtggttt gcgccagccg taacccgcgc
     6121 tgggcacggg attatcacac ggtccagatg ccaaaagagg tgcgtaaagc acgttatttc
     6181 agccgtcgtg aagagttgag cgaccctgag cttttgagcg cgattatctc ccggcgcgac
     6241 tactacaccg atgcctggtg gatggtggcg gtggcaacaa cagccgatgc gccttacagc
     6301 ctcgaacagt tgcaggacgg tttacgccat ccagtttttc cgctttatct ggggcgaaaa
     6361 agtcatccgc tggcattacc tcttgcgccg ttactgctcg aaggcaacgc gtgtgatgcc
     6421 ttatgtaatg cataccaaca gtatcaggat catttccata aactgaaagt ctcactcccg
     6481 aaacttcagg atgaatgctg gtgggaaggg aaacacgacg gcctggtcgc gagcaaaata
     6541 ttacgccgcc gggatgttcc tttaaatcgt cagcagtggc tgtttgggga acgcaccgtc
     6601 aatcaggggc cgtggctcag caaggaggaa ccatgtacct ctcaagaata accttacata
     6661 ccggccagct ttcgcctgcg cagttgctgc atctggtgga tcgcggtgaa tatgtgatgc
     6721 atcagtggct gtgggatctt ttccctggcg gcaaagaaag gcaatttctt tatcgtcggg
     6781 aagaactcc
//
LOCUS       DAZOXR010000206         6779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZOXR010000206 DAZOXR010000000
VERSION     DAZOXR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.213"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            52..936
                     /locus_tag="ACHUNN_003787"
     CDS             52..936
                     /locus_tag="ACHUNN_003787"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0578046.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHUNN_003788"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHUNN_003788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR0578047.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHUNN_003789"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHUNN_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0578048.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHUNN_003790"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHUNN_003790"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0578049.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="ACHUNN_003791"
     CDS             5160..6443
                     /locus_tag="ACHUNN_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR0578050.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..>6779
                     /locus_tag="ACHUNN_003792"
     CDS             6590..>6779
                     /locus_tag="ACHUNN_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR0578051.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSL"
BASE COUNT         1784 a         1667 c         1627 g         1701 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttat
//
LOCUS       DAZOXR010000207         6710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZOXR010000207 DAZOXR010000000
VERSION     DAZOXR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.214"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(159..644)
                     /gene="sixA"
                     /locus_tag="ACHUNN_003793"
     CDS             complement(159..644)
                     /gene="sixA"
                     /locus_tag="ACHUNN_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0578052.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(847..2991)
                     /gene="fadJ"
                     /locus_tag="ACHUNN_003794"
     CDS             complement(847..2991)
                     /gene="fadJ"
                     /locus_tag="ACHUNN_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0578053.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(2991..4301)
                     /gene="fadI"
                     /locus_tag="ACHUNN_003795"
     CDS             complement(2991..4301)
                     /gene="fadI"
                     /locus_tag="ACHUNN_003795"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0578054.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4481..4765)
                     /locus_tag="ACHUNN_003796"
     CDS             complement(4481..4765)
                     /locus_tag="ACHUNN_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0578055.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5137..6477
                     /gene="fadL"
                     /locus_tag="ACHUNN_003797"
     CDS             5137..6477
                     /gene="fadL"
                     /locus_tag="ACHUNN_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0578056.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1661 a         1807 c         1610 g         1632 t
ORIGIN      
        1 caagatacgc gcagaagaat aatttccgaa tgaaagtaga aagtgtttta tttatagatg
       61 gggagagtct tcgatagtag tgagattgac agatatccgc tgcctgatga gcaattatgc
      121 ccaatgaatc tacctcattg ggcataattt ggaactcatc agatagcttt tgccatcttc
      181 agattacacg ggctcatctg ccagttgaat gtgccgtttc cgctttcatc aagcgttacg
      241 ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc ccggacataa ctcggcaacc
      301 agatacccga ctaacggtaa gtgagagatc accagtaccg aagcaacacc ttcattggtc
      361 aacgcctgca aataggcact gaccagacca acatcgccgc agggcgttaa ctccggcaga
      421 acttctgcac tggaaggcag gttcagacaa tcccctactt cttccagtgt ttgctcggct
      481 cgcaggaacg ggctcaccag aacgcgttcg atttccactt tttgaccttt cagccagttc
      541 gccatcaggc gagattcgtc acaaccatta gtggtcagag gacgaaccga atcactggcg
      601 gcatcaaggg ctgcgtcgcc gtgacgcatg ataaaaactt gcatattgca ccgcttttgt
      661 taaccagatt cgtccgtttt ctttcatctg aaaccagaga aagaaaacgg tggccggcat
      721 tgtgccttat ccattcaccg aatgaaacgc tgttttttac ctcaatggcg taagtatagt
      781 caatatgcgt ttacattttg accaacactc cgccattcag cgcggattca tatagctttg
      841 accttcttat tgaaggtcag ttgcagttgt tttccaaaaa ctttccccac gcgcgcccat
      901 ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac tgcgtggcca gtcgttgcat
      961 tattgcaacc acttcgcccg cgccgagaga atcgatatag cggaacggtc caccgagaaa
     1021 tggcggaaaa ccaatgccaa ataccgcgcc aatatccccg tcacgcacgc tacggataac
     1081 ctgctcatca acacaacgta ctgcttcatt cagcatcaac atcacacacc gttcagcaac
     1141 ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg agcgggtaaa tggcgggatc
     1201 gacctgtttt ttgcttttac gccctttctg accataaaga tagaaacccc gaccattttt
     1261 tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca tttgcaggcg cgctaaaacg
     1321 ttctccataa gcggcttcca gtacaggaat aattttagtc ccggtgtcga ttcctacctc
     1381 atccaaaagt tggattgggc ctaccggaaa accaaatttc actagcgcgg catcaatgtg
     1441 ctctacccgt tcaccttcgg tcagcatgcg gatagcttca ttaatgtaag gcgctaagat
     1501 gcgattgacg taaaacccgg ctttgtcacg cacgacaatg ggcgttttgc cctgtttttt
     1561 cgccagtttt accgtagtgg cgatggtttg cgccgatgta cccgcatgag gaataatctc
     1621 taccagcggc attttttcca caggactgaa gaaatgcagg ccaataactt gctcaggtcg
     1681 cgcggcgtga gcggcgatat caccaatcgg taaagatgac gtgttcgaag caaaaatggt
     1741 atgagcggcg caattttgct ccacttccgc caccatctgt tgttttaatt cgagattttc
     1801 aaacaccgct tcaataatca gatcgcgatg ggcaaagccg cgatagtccg tcgttccgga
     1861 gattaatgcc agctgtttgt cacgttcgct ggctttgaga tgacgacggc gaactttgcc
     1921 ctccagctga tcccaactgt acttcagcgc atgatttatg ccctgcgggt tgatatcttt
     1981 aattctgacc ggaagccccg ctttacaagc agtgacataa gcaataccgc cgcccatcaa
     2041 gccaccacct aaaattccca cgctgtttaa tggcgcaggc ggtgcatcac taccgggatc
     2101 tttcttcacg tccgtactgg caaaaaagat actacgcaaa gcttgcgatt gcggcgtcat
     2161 cgccagttcg ccaaaggcgc gagcttcagc gtcatagccg ctgctggtgc cctgcgctaa
     2221 tcccgtttca acaacctcca ggatgcgttc tgtcgccgga taattgcctt gagttttgtg
     2281 ttctgttttc ttgccgacca ttttgaacag cagcgcacga cctaacggcc ccgccagaat
     2341 acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc ttcttcgcca gctcaacagc
     2401 ggcttccagc aaaatggagt gcggaacaac gtcatccacc agccccagct ttaatgcctg
     2461 tttcgcccga agttgttttc cggtgagaat catctctaat gctgtactga cgcctatcag
     2521 acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat ccaagttgta cttcaggcaa
     2581 accgagcacc gttttaggat cgtcagtaca aacgcgaccg tggcacgcca gcgccagctc
     2641 cagcccaccg cccaggcaag cgccatgaat agccgcgata accggaatgg gcaaagcatg
     2701 aatctccgcc atcaactgtt ggccctgccg cgccagtgct tccgcttctt gcgccgtttt
     2761 gcagttgcca atcatgttga tgtctgctcc ggcgataaaa ttgtccggtt tcgcggagat
     2821 aaacaccacg ccgcgcaact ctttgttttc acggagttgc ttaataatgg cgcgcacctg
     2881 cgaggcaaat tccgccttca gggtattcat tttctcatcc ggtacatcga tggtgatgat
     2941 agcaatgttg tccagacgaa cattaagagt aaacgctgat gccatttcca ttattccgcc
     3001 tccagaacca ttgccgcgcc aagcccaccg gcagcacagg cggtaactaa accaaatcca
     3061 ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca tccgcgcgcc ggtcgccgcg
     3121 aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa atttgctatc gtccacttcg
     3181 ccagtggcat gtgcacgccc cagtacttca cgagcaaaac gttcactacc cagcaactga
     3241 atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat cgatcaatgt cagttcgctc
     3301 atcgtcaaac cggcacgttc cagcgccagc ggtgttgacc aggctggacc gagcaacatg
     3361 tcctgccaga catcaatcgc agtaaatgcg tagctgcgca gataccccag cggcaccagc
     3421 cctaattctt tcgcccggga ttcagtcatc aggatcaccg ctgccgcgcc atcggtcagc
     3481 ggcgtactgt ttgccgccgt taccgttccg tgtttgcgat caaacgccgg gcgcagcttt
     3541 gcgtaatcgg caagcgagga attaccgcga atattgttgt cttcgacaag cggttgttta
     3601 taaggaggga taaaggcagt catcacctct tctttgagtt ttccgtctga ccatgcctga
     3661 gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct gttctcgggt gatgccgtag
     3721 gttttcgcca tttgttcggc ggtgtcgccc atccgcaagc cagtagaata ttctgctacc
     3781 gcaggcggta cgggcattaa gtcgcacaaa cgcaggcgag agaagagttt caggcgctgg
     3841 ctcatggttc gagctttgtt gacatcaacc agcacgcgcg ccagtttttt actgacgcca
     3901 attggcaata cggaagagga atctgcccca ccggcaatcc ccgctcggat agttcccgcc
     3961 atcaggcttt ctgcgacgtt tgcaaccgcc tggaaactgg tagcgcaagc gcggctgacg
     4021 ctgtaagcat cggtatgcac attcattccg gtaccgagaa caatttcacg cgcaatgttg
     4081 ggggcttcag gcatttgtac gacctgacca aagaccagct gttcaatcac ttcagcgggg
     4141 atctcgctgc gtgccagcag ttcgcctacc accatcttcc ctaaatcaac cgcgggaatg
     4201 ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta aaccgctaac aatggcgata
     4261 cgatcgccct ggcgggtaac cagcggtaaa acctgaccca taacactccc ctgtaaaaaa
     4321 ataaataaag tggtctgacc tgatcatagt cttaactatt tttttacatt tagccaagcg
     4381 gagaaaaagg aaagtgggag ctatgacaca gtgaagacag gagaaagaaa agaaaacgcc
     4441 cctgccagca actgacaggg gcgaacgtac ccgagaggaa ttaacgcaga cccagctgga
     4501 agatcagcat ttcagcttcg caggcaaaag tgaaatcgat atccaggcgc acaccgtcag
     4561 actcttcagt gaaagtcggg gagattttgc acggttcgga ttccacgcta cgggcttttt
     4621 cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt tgcaaacacg cggctgtaag
     4681 acgcggtgca gtcggagttg tccataatgg tgccaacatc catacagcag caaaccgggg
     4741 tttcatcagc actacattta ctcatcgttg atttcctctg tatgtgcacc caaggtgcca
     4801 gataaacgtt gtggatattt tacgcttccg gaaagtgctg ctccagttgt taattctgca
     4861 aaatcggata agtgaccgaa atcacactta aaaatgatct aaaacaaaat tcacccgaat
     4921 ccgcgagtgc gcaacctcca aattttgcca gctggatcgc gtttcttaga tcatatttga
     4981 aaaaagatgg aaacatactt gcagcattcc agctggtccg acctatactc tcgccactgg
     5041 tctgatttct aagatgtacc tcagacccta cacttcgcgc tcctgttaca gcacgtaaca
     5101 tagtttgtat aaaaataaat cattgaggtt atggtcatga gccagaaaac cctgtttaca
     5161 aagtctgctc tcgcagtcgc agtggcactt atctccaccc aggcctggtc ggcaggcttt
     5221 cagttaaacg aattttcttc ctctggcctg ggccgggctt attcagggga aggcgcaatt
     5281 gccgatgatg caggtaacgt tagccgtaac cccgcattga ttaccatgtt tgaccgcccg
     5341 acattttctg cgggtgcggt ttatattgac ccggatgtga atatcagcgg aacgtctccg
     5401 tctggtcgta gcctgaaagc cgataacatc gcgcctacgg catgggttcc gaacatgcac
     5461 tttgttgcac cgattaacga ccaatttggt tggggcgctt ctattacctc taactatggt
     5521 ctggcaacag agtttaatga tacttatgca ggcggctctg tcgggggtac aactgacctt
     5581 gaaaccatga acctgaactt aagcggtgcg tatcgcttaa ataatgcatg gagctttggt
     5641 cttggtttca acgccgtcta cgctcgcgcg aaaattgaac gtttcgcagg cgatctgggg
     5701 cagttggttg ctggtcagat tatgcaatct cctgccgggc aaactcctca agggcaagca
     5761 ttggcagcta ccgccaacgg tatcgacagt aataccaaaa tcgctcatct gaacggtaac
     5821 cagtggggct ttggctggaa cgccggaatc ctgtatgaac tggataaaaa taaccgctat
     5881 gcactgacct accgttctga agtgaaaatt gacttcaaag gtaactacag cagcgatctt
     5941 aatcgtgcgt ttaataacta cggtttgcca attcctaccg cgacaggtgg cgcaacgcaa
     6001 tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag tgtcaggtta taaccgtgtt
     6061 gatccacagt gggcgattca ctatagcctg gcttacacca gctggagtca gttccagcag
     6121 ctgaaagcga cctcaaccag tggcgacacg ctgttccaga aacatgaagg ctttaaagat
     6181 gcttaccgca tcgcgttggg taccacttat tactacgatg ataactggac cttccgtacc
     6241 ggtatcgcct ttgatgacag cccagttccg gctcagaatc gttctatctc cattccggac
     6301 caggaccgtt tctggctgag tgcaggtacg acttacgcat ttaataaaga tgcttcagtc
     6361 gacgttggtg tttcttatat gcacggtcag agcgtgaaaa ttaacgaagg cccataccag
     6421 ttcgagtctg aaggtaaagc ctggctgttc ggtactaact ttaactacgc gttctgataa
     6481 cgcgttcgcc tggataaagt cacctgcata gcaggtgact ttaactcccc cacttcaccg
     6541 aaagtagtgt cctcattgct tacctcctga gttttgcaaa cagcctgttg gcagcttgcc
     6601 ctttcaaatc aataagcggt atccacagac aaacctgaag ggaaaggcat attttcaggc
     6661 gttctgctcg tccttctcaa agagtttact tttctgcatt tccaggatac
//
LOCUS       DAZOXR010000208         6614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZOXR010000208 DAZOXR010000000
VERSION     DAZOXR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.215"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            165..851
                     /locus_tag="ACHUNN_003798"
     CDS             165..851
                     /locus_tag="ACHUNN_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0578057.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(942..1078)
                     /locus_tag="ACHUNN_003799"
     ncRNA           complement(942..1078)
                     /ncRNA_class="other"
                     /locus_tag="ACHUNN_003799"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1123..1210)
                     /locus_tag="ACHUNN_003800"
     tRNA            complement(1123..1210)
                     /locus_tag="ACHUNN_003800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1174..1176),aa:Ser,seq:gga)
     gene            1444..2382
                     /gene="ghrA"
                     /locus_tag="ACHUNN_003801"
     CDS             1444..2382
                     /gene="ghrA"
                     /locus_tag="ACHUNN_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR0578058.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2437..3174
                     /gene="ycdX"
                     /locus_tag="ACHUNN_003802"
     CDS             2437..3174
                     /gene="ycdX"
                     /locus_tag="ACHUNN_003802"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0578059.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3198..3752
                     /gene="ycdY"
                     /locus_tag="ACHUNN_003803"
     CDS             3198..3752
                     /gene="ycdY"
                     /locus_tag="ACHUNN_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0578060.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3854..4345
                     /locus_tag="ACHUNN_003804"
     CDS             3854..4345
                     /locus_tag="ACHUNN_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0578061.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4410..5243)
                     /gene="csgG"
                     /locus_tag="ACHUNN_003805"
     CDS             complement(4410..5243)
                     /gene="csgG"
                     /locus_tag="ACHUNN_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR0578062.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5270..5686)
                     /gene="csgF"
                     /locus_tag="ACHUNN_003806"
     CDS             complement(5270..5686)
                     /gene="csgF"
                     /locus_tag="ACHUNN_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0578063.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5711..6100)
                     /gene="csgE"
                     /locus_tag="ACHUNN_003807"
     CDS             complement(5711..6100)
                     /gene="csgE"
                     /locus_tag="ACHUNN_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0578064.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6105..>6614)
                     /gene="csgD"
                     /locus_tag="ACHUNN_003808"
     CDS             complement(6105..>6614)
                     /gene="csgD"
                     /locus_tag="ACHUNN_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0578065.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         1657 a         1511 c         1640 g         1806 t
ORIGIN      
        1 atttttttgt ggaaggcaag ccgcaatggc taaataggga tggttgtaat aatcaaaaca
       61 tgatactaac tatatattga tcgttgcatt gttggcatgt aacacgctgc agcgagttac
      121 attggtaatg aaatataatc aacatggata ggtgaaaatt aattatgagc tttatggctt
      181 ttgtaacttg tttgacaatt gcatttatcg ccggttttgc tgttgccaga aaattgacac
      241 tccgaaaagt tgcctcatcg gtaatcatcg ctaaatcgca acccgtccct tcatctgcaa
      301 cttcccctgc cgctaaggcg tcgacaagga gctatatacc gcagaaaacc tatcgtgact
      361 ctcgttggca agagcgagat gagttatttc agggaatggt tgccgaaggt attttattgg
      421 aaagaaaaga gtggttaatc acgcgttgtg aaaaaatgta tttcgataat ctggaaaaat
      481 ggtttggaca ttattgtcat atccattgcc aggtctctct ggggcgcttg atcaactttc
      541 cagaacaacc tgagtttagc gaagaagaaa gaaagcgttt ctttgcaatt tataatggca
      601 tggcaatgga ttatgttctg gtttcaaaga agaacaataa aatagtctgt gttattgagc
      661 tggatgaccc gagtcatgaa tgggcggatc gcattattcg tgaccgttgg ttggatacaa
      721 ttatgactct tgcacgggtg ccttttgtac gagcttctgt cactaaattg gaggaaattc
      781 ctccggtctg ggagactcgt gacaaaattc gcccattagt cgcggcgaca aagagtgaac
      841 cggtgcatta actaagtatc ccggtcgggg cttctcatcc ccccggtgtg tgcaatatac
      901 gaaaaaaagc ccgtaccttc gtacgagctc ttctttaaag atggcggtga gggggggatt
      961 gactcgcttc gctcgccctg cgggcagccc gctcactgcg ttcacggtct gtccaactgg
     1021 ctgtcgccag ttgtcgaacc ccggtcgggg cttctcatcc ccccggtgtg tgcaatatac
     1081 gaaaaaaaag cccgtacttt cgtacgagct cttctttaaa tatggcggtg agggggggat
     1141 tcgaaccccc gatacgttgc cgtatacaca ctttccaggc gtgctccttc agccactcgg
     1201 acacctcacc aaattgtcgt tcctgtcttg ctggaacggg cgctaattta gggaaatcat
     1261 gacctgaggt caacaaactt tttgaaaaaa tcgcgcgttt attcaaactt caatcaatgt
     1321 gtggttttaa taagcgaaat ctgctttttt tgccaccgac cacggatttg ttatgctggt
     1381 ggcctttgta gatcataacg ataagtgcga ataaatttcg cacaacgctt ttcgggagtc
     1441 agtatggata tcatctttta tcacccaacg ttcgataccc aatggtggat tgaggcactg
     1501 cgcaaagcta ttcctcaggc aagagtcaga gcatggaaaa gcggagataa tgactctgct
     1561 gattatgctt tagtctggca tcctcctgtt gaaatgctgg cagggcgcga tcttaaagcg
     1621 gtgttcgcac tcggggccgg tgttgattct attttgagca agctacaggc acaccctgaa
     1681 atgctgaacc cttctgttcc actttttcgc ctggaagata ccggtatggg cgagcaaatg
     1741 caggaatatg ctgtcagtca ggtgctgcat tggtttcgac gttttgacga ttatcgcatc
     1801 cagcaaaata gttcgcattg gcaaccgctg cctgaatatc atcgggaaga ttttaccatc
     1861 ggcattttgg gcgcaggcgt actgggcagt aaagttgctc agagtctgca aacctggcgc
     1921 tttccgctgc gttgctggag tcgaacccgt aaatcgtggc ctggcgtgca aagctttgcc
     1981 ggacgggaag aactgtctgc atttctgagc caatgtcggg tattgattaa tttgttaccg
     2041 aatacccctg aaaccgtcgg cattattaat caacaattac tcgaaaaatt accggatggc
     2101 gcgtatctcc tcaacctggc gcgtggtgtt catgttgtgg aagatgacct gctcgcggcg
     2161 ctggatagcg gcaaagttaa aggcgcaatg ttggatgttt ttaatcgtga acccttaccg
     2221 cctgaaagtc cgctctggca acatccacgc gtgacgataa caccacatgt cgccgcgatt
     2281 acccgtcccg ctgaagctgt ggagtacatt tctcgcacca ttgcccagct cgaaaaaggg
     2341 gagagggtct gcgggcaagt cgaccgcgca cgcggctact aataaagcat caggattcct
     2401 gctatccttg gcgggaattg aatacaggag agagttatgt atcccgtcga ccttcatatg
     2461 cataccgttg ccagcacaca tgcatatagc acattgagtg attacattgc ccaggccaaa
     2521 caaaagggca ttaaactttt tgcgatcacc gatcatggcc cggatatgga agatgctccg
     2581 catcactggc acttcattaa catgcgtatc tggccgcgag tggttgatgg ggtagggatc
     2641 ctgcgcggca tcgaagctaa cattaaaaat gttgatggtg aaattgattg cagcggtaaa
     2701 atgtttgact cgctggatct gattattgcc ggttttcatg agccggtttt tgcaccacat
     2761 gacaaagcga ccaatacaca agcgatgatc gccactatcg ccagcggcaa tgtgcatata
     2821 ataagccatc ccggaaatcc caaatatgaa atagatgtga aagccgttgc tgaagcagcc
     2881 gcgaaacatc aggtggcgct ggaaatcaat aattcttcat ttttacactc acgtaagggc
     2941 agtgaagaca actgtcgtgc ggtagccgca gcggtacgtg atgctggagg ttgggtggca
     3001 ttaggctcgg attctcacac cgcgtttacc atgggggaat ttgaagagtg tcttaaaatc
     3061 ctcgacgcgg tagattttcc gccagagcgc attttgaatg tttctccgcg ccgcttactg
     3121 aacttccttg aatctcgcgg tatggcaccg attgcggaat ttgcagacct ttaattacta
     3181 gtaatggaaa tatattaatg aacgagtttt ctatcctctg tcgtgtgctg ggttcgctct
     3241 attaccgcca accgcaagat cctttactgg tgccgctgtt taccctgatt cgtgagggga
     3301 aactggctgc gaactggcca ctggagcagg atgagttact gacgcgttta cagaaaagct
     3361 gtgatatggc gcaagtctct gccgattaca atgcgttgtt tatcggcgat gaatgtgcag
     3421 tgccgccata tcgtagcgca tgggttgagg acgtaacgga agcggaagtg cgcgcttttc
     3481 tttccgaacg agggatgcca ctggcggata cgccagccga tcacatcggc acattgctgc
     3541 ttgcggcttc ctggctggaa gatcagtcaa cggaagatga gagcgaagca ctggaaacac
     3601 tgttcagtga gtatctgtta ccctggtgtg gtgcgttcct cggcaaagtg gaggcccatg
     3661 caaccacgcc tttctggcgc accatggcac cgctaacccg tgatgccatt agtgcaatgt
     3721 gggacgagct ggaagaagat tctgaagagt aaatgtgatc atcgtcactc ataactgcaa
     3781 cagataattt gttgttgcat aaaatgtgtg ctcgatctca ttcatggccg cgttttctgc
     3841 tatcatgcgc ggcatgaaca tacttctctc tattgcaatc acaacaggca ttctctccgg
     3901 tatctgggga tgggtggctg tttccctggg cttactaagc tgggcgggct tcttgggctg
     3961 tacggcctac tttgcctgcc cgcaaggtgg gctgaaaggg ctggcgatct ccgctgcaac
     4021 cctgcttagt ggcgtggtgt gggcgatggt cattatttac ggtagtgcac tggcaccaca
     4081 tctggaaatt ctcggttatg tcataaccgg tattgtggcg tttctgatgt gtattcaggc
     4141 caaacagctg ctgctttcat ttgttccggg gacgtttata ggcgcatgcg cgacgtttgc
     4201 cgggcagggt gactggaaac tggtgttacc ttcgctggcg cttgggctgg tatttggtta
     4261 cgcaatgaaa aacagcggcc tgtggctggc ggcgcgtagt gcaaagacgg ctcaccgtga
     4321 gcaggaaatc aaaaataaag cgtgaggggc actcacgctt tcgcttaaac agtaaaatgc
     4381 cggattatta attccggcat ttttatctgt caggattctg gtggaaccga catatggcgg
     4441 tatttaacca gaatgtcatt ctgccgttct gctttgttct gcaaatccca cagaccacgg
     4501 tcgataccat cattaatcag gaaaatgacc cctgtttcga tagccgacat cagacacagc
     4561 ataacgggtt cgtttgaggt gtaacccact tccccttcca gcaagcgctg gtagtcaata
     4621 aagcggaaaa ccccggcctg aacttcatag gaaagtatcg tcttactggt gttcaccgag
     4681 gagaggatct caccggtact cacattgacg acacgcaggt tcacggcaat ctgatcgagc
     4741 tggtattgcg tgtctgcacc gatcccaaaa tatcttgccc caacaccgcc cgatttaacg
     4801 ttgctttcat aaccgataat cgaaccttca accatgatat ttgccgccgt taaagattgc
     4861 agcgggattc ggttattaat ggcaactgtg ccgttttctt gtgccgcacg aataatcttg
     4921 cgctcgttaa gcaggttttg taaactctga cgctccagcg gtataaacca gcgagaatct
     4981 ttcaatgccg tgaccagcat tgccgtggcg ctttgcggaa cagcagtgga gaagttactt
     5041 gccgggtagg gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc
     5101 ttacccgtcg gcgctggcag atgggtcaaa tctttgtagc tctgagctcg aggcattaat
     5161 gtcggtctgg cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca
     5221 accaaaagaa ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg
     5281 ttgagttatt ttgtaaaccc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg
     5341 tcacgttcaa ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca
     5401 ccatgcggcc cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact
     5461 ggatggcctg agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat
     5521 agctcggatc tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat
     5581 ttgggttacc accaaagttt ggattacgga actggaaagt catggttcca gcccaactta
     5641 atggcgaaat aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac
     5701 aattttttat ttagaattca tcatgcgcca aatcgcccgt acttaataac gcctgattta
     5761 tctggcgacg atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcga
     5821 agtctctttt caacggaaat aaaaaagtct ggaaaataac gtcctgattg accgttatag
     5881 tgatccagct tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt
     5941 cactttccca tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat
     6001 ggtcagttag caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca
     6061 gaaattctgc cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta
     6121 tcgtttgccc aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta
     6181 taaagatgcg ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta
     6241 gacgcgccga tacgcagttt attcaggatc tctttttccc gatgagtaag gagggctgat
     6301 tccgtgctgt tataacgata gttacctgaa tgcgtaatca agtagctggc aagcttttgc
     6361 gtaaagtagc attcgccgcg caggacgcct tgcaacccat tgacaacacg ttcttgatcc
     6421 tccatggcat aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa
     6481 tcttcaggcg tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc
     6541 tgccaataat ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct
     6601 gaagatatat cgtc
//
LOCUS       DAZOXR010000209         6537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZOXR010000209 DAZOXR010000000
VERSION     DAZOXR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.216"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUNN_003809"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUNN_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0578066.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUNN_003810"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUNN_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0578067.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHUNN_003811"
     CDS             2408..3157
                     /locus_tag="ACHUNN_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0578068.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUNN_003812"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUNN_003812"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUNN_003813"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUNN_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0578069.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUNN_003814"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUNN_003814"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0578070.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="ACHUNN_003815"
     CDS             6018..6491
                     /locus_tag="ACHUNN_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR0578071.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAZOXR010000210         6515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZOXR010000210 DAZOXR010000000
VERSION     DAZOXR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.217"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHUNN_003816"
     CDS             38..250
                     /locus_tag="ACHUNN_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578072.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHUNN_003817"
     CDS             283..501
                     /locus_tag="ACHUNN_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0578073.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHUNN_003818"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHUNN_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUNN_003819"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUNN_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0578074.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHUNN_003820"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHUNN_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHUNN_003821"
     CDS             1917..2660
                     /locus_tag="ACHUNN_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0578075.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHUNN_003822"
     CDS             2690..3229
                     /locus_tag="ACHUNN_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0578076.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUNN_003823"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUNN_003823"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0578077.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUNN_003824"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUNN_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0578078.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHUNN_003825"
     CDS             4582..6102
                     /locus_tag="ACHUNN_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0578079.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="ACHUNN_003826"
     CDS             complement(6135..>6515)
                     /locus_tag="ACHUNN_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578080.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DAZOXR010000211         6459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZOXR010000211 DAZOXR010000000
VERSION     DAZOXR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.218"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUNN_003827"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUNN_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0578081.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUNN_003828"
     tRNA            1230..1305
                     /locus_tag="ACHUNN_003828"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUNN_003829"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUNN_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUNN_003830"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUNN_003830"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0578082.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUNN_003831"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUNN_003831"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0578083.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUNN_003832"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUNN_003832"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0578084.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="ACHUNN_003833"
     CDS             complement(4869..5306)
                     /locus_tag="ACHUNN_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR0578085.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6459)
                     /locus_tag="ACHUNN_003834"
                     /pseudo
     CDS             complement(5788..6459)
                     /locus_tag="ACHUNN_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
BASE COUNT         1723 a         1749 c         1418 g         1569 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaa
//
LOCUS       DAZOXR010000212         6368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZOXR010000212 DAZOXR010000000
VERSION     DAZOXR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.219"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..358
                     /gene="ydjO"
                     /locus_tag="ACHUNN_003835"
                     /pseudo
     CDS             1..358
                     /gene="ydjO"
                     /locus_tag="ACHUNN_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(362..1753)
                     /gene="tcyP"
                     /locus_tag="ACHUNN_003836"
     CDS             complement(362..1753)
                     /gene="tcyP"
                     /locus_tag="ACHUNN_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR0578086.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1886..2476)
                     /locus_tag="ACHUNN_003837"
     CDS             complement(1886..2476)
                     /locus_tag="ACHUNN_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0578087.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2639..3307)
                     /gene="hxpB"
                     /locus_tag="ACHUNN_003838"
     CDS             complement(2639..3307)
                     /gene="hxpB"
                     /locus_tag="ACHUNN_003838"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0578088.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3454..3990
                     /locus_tag="ACHUNN_003839"
     CDS             3454..3990
                     /locus_tag="ACHUNN_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0578089.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4031..4891)
                     /locus_tag="ACHUNN_003840"
     CDS             complement(4031..4891)
                     /locus_tag="ACHUNN_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0578090.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4997..5287)
                     /gene="ghoS"
                     /locus_tag="ACHUNN_003841"
     CDS             complement(4997..5287)
                     /gene="ghoS"
                     /locus_tag="ACHUNN_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0578091.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5388..6317)
                     /gene="pfkB"
                     /locus_tag="ACHUNN_003842"
     CDS             complement(5388..6317)
                     /gene="pfkB"
                     /locus_tag="ACHUNN_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0578092.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1645 a         1597 c         1561 g         1565 t
ORIGIN      
        1 cctgccggga gcatttttgc tgcgattcaa aaaaaatgag agtctcctaa agtttccttc
       61 gataatatca atgcttaata tcaatcttct ttttaatcca ctaaatcttc cgggaatggg
      121 aagtggtgtg cttgaagata ttatgagtat tccagatagt tcacttagaa aacgtctcgg
      181 gtatgaggtt ttaagcttct cactacaggc acattcattg agccaggaat gtattgataa
      241 gttggacatt tttttcgctg acgatctgtt taaatatgag tcagtatgca tcgccgcgat
      301 ggaacattta aaaagtaaag caacggcccc tatacaaaac ggaccgttgc cagcataaga
      361 attaacggtg tgccagttcg gcgtcgtctt cgctatccag aatggcttta tcggtttgct
      421 tcagccactg gctggtcagc gtgccagctg tcatcgagcc actaacgttt agcgccgtac
      481 ggcccatgtc gataagcggt tcaacggaga ttaacagcgc caccagggtt actggcaggc
      541 ccatcgcagg cagcacaatc agtgcggcga aagttgcacc accaccgaca ccggcaacgc
      601 ctgcggaact aacggtaaca ataccgacca gcgtcgcaat ccacatcggg tccagcgggt
      661 taatgccaac cgtaggcgca accatcaccg ccagcattgc cggatacaaa ccggcgcagc
      721 cgttctgacc aatggttgca ccgaaagagg cggcgaaact ggcgatggat tcaggaacgc
      781 ccagacgacg cgtttgtgct tccacattca gtgggataga cgcagcactg gaacggctgg
      841 taaaggcaaa cgtcagcaca ggccatacct tacggaagta cttcagcgga ctcacgccat
      901 taatgcccag cagaatgcca tgcactgcaa acataatcag cagaccgagg taggacgcga
      961 caacgaaact tcccagtttg atgatgtctt gcaagttaga acctgcaacc actttggtca
     1021 ttagagccag aacgccgtaa ggggtcaact gcatgacaag gcgaaccagt ttcatcaccc
     1081 agctttgtag ggtatcgata gcggctaaga cgcgttcacc tttcggcgca tcatccttca
     1141 gcagtttcaa tgcagctacg ccgaggaatg cggcaaaaat taccacgctg ataattgacg
     1201 tcggattggc tccggtaaga tcggcaaacg ggtttttcgg gataaaggac aagaccagct
     1261 gcggaacgct cagatcagag actttaccaa catagttact ttcaatggcg ttcagacgtg
     1321 cagtttctgc accaccctga accagacctt cagccgtcaa accaaacagg ttggtgacca
     1381 gcacaccgac cagcgccgca atcagcgtgg taaacaaaag cgtaccgatg gtcagaaaac
     1441 tgattttgcc taactgagat gcgttatgca gacgggcaac cgcgctcaga atagaggcga
     1501 agactaacgg cataacgatc atttgcagca gttgaacata gccgttacca acgatgttaa
     1561 accactgtac agaatcttta agtacctggc tgtcagaacc ataaatggta tgcagggcaa
     1621 ggccaaaaac cacacccatc accagaccca ccagcacttt tttcgccaga ctccactgtt
     1681 tatggcgggt ctgagccagc gcaaacagca gtacaacgaa caccacgatg ttcgcaatta
     1741 atggaaagtt catccccgtt ctcctgatct tattatttct atcactgcgt agcagtcata
     1801 gtgttggcgc aagattaaca gaactgtgag ctggctctta tattcaaatg gaattgctta
     1861 ttccagaaag gtgatttttg cctttttagt attcaacctg atgcttaata agccgatgaa
     1921 actggatctg caaggtattg atcatttgcg atgaccaacg aacagcgctg ttctcgggta
     1981 atgaatgggg catccatacc gaccagacaa ataatgccat gcagataaaa cgttccagtt
     2041 ggttgccctt ttggggaacc aggataggca agcggaaacg ccagcggcat ggccagagca
     2101 ggggaacacc ggcgggtgtc agcatatcgg caagtatgtg gctcaaataa cccagcacca
     2161 ttccttgtag cgcatcagcc ggaatgaacc agccttccgg aaccttaagg taaaaggttg
     2221 ccagcagcgc aaataccgcc agcagactgt gggtaaaacc acgatgccca aaagcgcggg
     2281 cgatcggttt tgatatccat tttaatcgct gcccaagaaa cgactttggg tgatcgatgt
     2341 ccggtaacaa acacgtcagg attgcggaag ggacaatatg ccaccagtca ccctgtgcca
     2401 gcacgggcgt cagctcggca tttttggcaa ataccgcaca agcaatagaa aagagaaggt
     2461 gaccttccgc cgtcatgata aaactcacta aactgtcgat acgtacagta taggattttt
     2521 atacagtgca cggaagtggt gacggtgtaa ctctttctct taaattgacg attaatccgt
     2581 cagttgaatg caaattcaga taaagaacca ggggcagcgt gatcactgcc cctggtcatt
     2641 aaccgagaag gtcttttgcg gtgagttctg tcagcgatga gagtttgacg tttgctaata
     2701 caaaacgtgg atcattttgc gcttctggcg caggaacgac gatggaacgc atgcgggctg
     2761 ctttagaagc gatcatgcca tttaccgagt cttccagcgc cacgcaggtc agtgggtcaa
     2821 cgcccagttt tgctgcgcag tcgagatata cttgcggatg cggcttgctg taaggcagtt
     2881 tttcagccga ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt
     2941 ccagcatatg tagtggtgac gcggaggcca gtcccaccaa taaaccttgt tctttgcata
     3001 acgcaacagc ttcgcgcacg cctggtaata atggacgtgt ctcttcaacc agtgaaatgg
     3061 cacgggcaat aacccgttct actacttcct gacggcttgg cccattccac ggttgccggg
     3121 cgtaccaaag atcgaccacc atatcgatgc gtaaacctaa ggtgtccggc agctcgttac
     3181 gacgggagat atccaccccc aggcttgcca tcacatccag ttcggctcga tcccgtaaag
     3241 gttctgagtc gataagtaat ccatccatat caaaaattgc agcaagaatc tgacgcgggg
     3301 ttgacattac aacctctcct ttagagcatt atgtgtaagg tattaagccg tgcttatgat
     3361 taagcgccta tcatatcgcc tttaaagaat gggcgaaaac agatatctac aggtaaacta
     3421 agtgaaacga cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt
     3481 gctgttttga aaaggatttt gggttgggtg atttttatac ctgcattgat ctccacattg
     3541 atttctttgc tgaaatttat gaatacccgg caggaaaacc aggaaggcat taatgcggtc
     3601 atgctcgact ttactcatgt catgatcgat atgatgcagg cgaatacgcc ttttttgaat
     3661 ctgttctggt ataactcccc gacccctaat tttaatggtg gcgtgaacgt tatgttctgg
     3721 gtgattttca tcctgatttt tgtcggactg gcgttgcagg attccggtgc ccggatgagt
     3781 cgccaggcgc gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa
     3841 ggggaagagg ggctaacgcg tgaacaaatt gagtcccgta ttgttgttcc gcaccatact
     3901 attttcctgc agtttttctc actatatatc ctgccggtta tttgtattgc tgcaggttat
     3961 gtgttctttt ctctgcttgg gtttatttaa tcaaaaggcc gcacggagcg gccattatcc
     4021 tcaacccata tcatgctgct aataatctat ccaatgactg ctgagcaata accaaatgct
     4081 gaccgccaaa taatcttgca cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt
     4141 caaggaaatc ggcaggtagc ggggatactg actgatagcc gtcatagatt tgtggcggct
     4201 gttcagtatg cagcggtaac atcgccaggt cgcactctcg gtcaccccag tagcaggcag
     4261 ggtcgaaaat gtacgggcca tccggaccca gcgcacagtt gccggaccat aaatcgccgt
     4321 gcaacagaga aggctgcagt tgatgcgagg ccagacgctg ctgaatatgc tcgacgatgg
     4381 cgtcgatatt gccgaaagcg atccctttct ctgctgccag ttccaactgc cagccaatcc
     4441 gttgttcagc aaaaaacgtt gaccagcgac gttgccaggt gttgggctgt ggagttgtgg
     4501 agagcgagtt atcaaaatcg aggccaaatt gtggttggtc actccattga tgtaaacgcg
     4561 caatttgctg accaagaata aatgcgctat gcgcatccag cggacgaggt gggagataat
     4621 ccatcaccag aaaactgtag tcacggtcag cgcctactgc ccaaacctta ggcacggtga
     4681 cggttttact acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca
     4741 gttccctttc atcacatttg acgaaaaagt catgtcctgc atagcgcaaa tgccatgcgg
     4801 catggacttc tccgccaggc agttcattac gcagttcgat ttcgccttca cctaactgct
     4861 cgctcaaaag acgactgatt gcctgccaca tgatgtctct cccatgttgt ctgccagatc
     4921 ataaggttag cgcaagaatg cggtgtaaaa acacgagcta acgcacacca cagactgatt
     4981 attgcccaaa aaccatttat tttctgttgc tattccattc ctcccaagtc gtgatggtga
     5041 tttcaggatc tttgccggtt gcactttggg taagccccga aaccagttct ctaatttctt
     5101 cttcactttg ggtactgata agtccaaaag tgttagtccc cagctcatgg atatttccct
     5161 catcatccgt catggtgagc aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa
     5221 tttcagtcaa tgtatcctca tgcaacatga cggttatgac ataacggaca agatcgccac
     5281 tggccatagt tcaccccttt gttatcacat gacttttgag caaagtcgga gaaacgcgtt
     5341 gccgacaggt tggtggtgat tcccccaatg ctgggggaat gtttttgtta gcgggaaagg
     5401 taagcgtaaa ttttttgcgt atcgtcatgg gagcacagac gtgttccctg attgagtgtg
     5461 gctgcactcc ccgcagctac gccaaaacgg accatctctt caagagaggc attttctgcc
     5521 agtttcagtg tcatcgcgcc gaccatgctg tcaccagcgc caacggtact ctggcttttc
     5581 accggtggtg gcaccacctg aatgcagttt tcactatcaa cacccagcgc tccttgtgga
     5641 ccaagggaaa cgacaacccg tttggccttg ccgctattaa cgatttcctg cgcggctttg
     5701 cggacatcgt ccggctgggt gagttcgcga ttcaccagcg cactgagttc tttttggtta
     5761 ggcttaacca actcgatgtt accaattgcc agtgctgcac ttaacgcttc gccagaactg
     5821 tcgacgatgc agcggatccc ttgtttttgc gaagcggaaa tcagttgggt taatttttcc
     5881 agcttcacac ctggcggcag gcttccgctt atgaccagga tggccccgga ttcaatttcc
     5941 agaacttgct cttcaagctg gcgaaactca tcttcattta atgccgcgcc tggcataaca
     6001 aaacgatact gctcaccgct tgcttccaca tgtacatgta aattctgccg ggtccagtct
     6061 ttggcttcta cagtagcgac ggggacattt tcatccgcca acagtgaaac caggtgttcg
     6121 ccggtcgcgc cacccgccgg gaagatcgct gtggcactgc ctccaagatg ggcaatggcg
     6181 cgggcgacgt tgatgccgcc gcccccgggt tcgaacaccg gtgcggtaca gcgcagtttt
     6241 ccttcgggat aaatttgcgg ggtaattgtt gcgctatcga gagagggcgc aagtgtcaac
     6301 gtatagatac gtaccatcat ttcctcctat aggctgattt cagtctggca ccgaatcagc
     6361 ggaaagtg
//
LOCUS       DAZOXR010000213         6327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZOXR010000213 DAZOXR010000000
VERSION     DAZOXR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.220"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHUNN_003843"
     CDS             <1..331
                     /locus_tag="ACHUNN_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0578093.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUNN_003844"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUNN_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0578094.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUNN_003845"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUNN_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0578095.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUNN_003846"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUNN_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0578096.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUNN_003847"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUNN_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0578097.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUNN_003848"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUNN_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0578098.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUNN_003849"
     CDS             complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUNN_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR0578099.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1710 a         1500 c         1536 g         1581 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaaca
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc ggcgagcagg
     6241 gcaggaatta aaagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     6301 ttgcctttta tccccattcc ggggagt
//
LOCUS       DAZOXR010000214         6325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZOXR010000214 DAZOXR010000000
VERSION     DAZOXR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.221"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..355
                     /gene="mcbR"
                     /locus_tag="ACHUNN_003850"
     CDS             <1..355
                     /gene="mcbR"
                     /locus_tag="ACHUNN_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0578100.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHUNN_003851"
     CDS             complement(391..2493)
                     /gene="pqqU"
                     /locus_tag="ACHUNN_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR0578101.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2735..3796
                     /locus_tag="ACHUNN_003852"
     CDS             2735..3796
                     /locus_tag="ACHUNN_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR0578102.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHUNN_003853"
     CDS             complement(3911..5410)
                     /gene="ansP"
                     /locus_tag="ACHUNN_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR0578103.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5677..6294
                     /locus_tag="ACHUNN_003854"
     CDS             5677..6294
                     /locus_tag="ACHUNN_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR0578104.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1514 t
ORIGIN      
        1 caccccgcaa gacctgacgg aactccagga attgctcgag aaattacagc aggcgcaaga
       61 aaagggcgac atggaacaaa tcattaatgt aaacaggcta tttcgcttag cgatttatca
      121 tcgctcaaat atgcccatcc tgtgtgagat gattgagcaa ctgtgggtca ggatggggcc
      181 tggtttacat tatctctatg aagcgattaa tccagcggaa ttacgggagc atatagaaaa
      241 ctatcatctg ttactcgctg cgttaaaagc aaaagacaaa gagggatgca gacattgtct
      301 tgctgaaatt atgcaacaaa atattgctat tttataccag caatacaatc gttaaagtaa
      361 atgcccggca aataccgggc atcaaggtaa ttactcaaat ctccacgcaa tattcatgcc
      421 gacaccataa tttcgtccgg gcgaaggttc gtaatatcgc ccgtttgact cattgacaat
      481 gacagaacca acgtattctt tatcgaataa attatcgaca cgaccaaata aatccacagt
      541 taaattgtgg taattgtatt tatacccggt gaataagccg acgagagtat aagacggcgc
      601 ttttgccgta ttttcatcat ctgccataat atcgcccata taacgcgctt ccgtgcctgc
      661 ataccaacca tcttccggta cataacctat cgacgcaaag cccatattac gggcgatccc
      721 tggcatccga ttaccgttac aatcctgttc attgcaaaca ttgctgcgat aggtcgcatc
      781 aagccaggtc caggacgcgt ttacgcgaaa atctcctgcg aaacgttgat cccatgccag
      841 ttcagcgcct tgacgacggg tctttccggc atttttgtaa gtcgtacgcc caccgctact
      901 gctatcgaca acaatttcat catcagtgtc ggtctgaaac aatgcgagac taagcagccc
      961 atcaccaata cgcgttttac tgccgatctc aattgtatcg ttggtggatg gttttaaacc
     1021 aaagttcata ccgctttgcc catcagcacg ataagacagc tcattaatcg tcggcgtttc
     1081 aaaacctcgc ccggctgcca gatagatatt ccaggcatcg gtcattgcat attttaacga
     1141 accggcaggt agccatttat gataactggc atcaccgctg tcatcgccgt tacccggagt
     1201 aacgtaatgg tcgttggaat caaaccacac ggagctgtag cgcacgccag catccagcga
     1261 cagtttttcg ctcagctgcc actgcgtctg taaataggga tcgatgttcc acatcagatt
     1321 gcgttcgtcg cgacgcaact cacctttttg cccgtactcc ggcatgccgc tattcaggcg
     1381 gaagttattg tagcccttgc ggttttcact catgttttcg tagttcaggc cggtagtgaa
     1441 cgtgaccgga acgcccagtt caccacggtg tgtccagcgg ctgtctatcc cctggtaatg
     1501 gcgttgcagg gtaatcacgc cgcccgcatg tgacgggtta agttgtggtg ccatgggtat
     1561 tgactggtac tgggtcgttt ctcgctctcc ggcatacatc atcacactca tatcatcttg
     1621 cgcgctcagg ctacgctcat agcgcaaccc agcctgagtt tgcttgatgg tttttcgcgt
     1681 gtcgtactgt tctgcacgag gcgcttgttg tggattagcc ttccattctg ctttggttag
     1741 cccacccggg tcatctgctt tgatatccac actattgaaa atcagactta atttgctggc
     1801 ttcatcaatg cgtacgccca gtttggcatt ggctaaattt ttctgtgcgc cactatggtc
     1861 acgatagccg tgggtcgtaa aacgcgtggt tgagacggtg taatcgacat cgccaggctg
     1921 tgtgccgtct cccgttgcgc ccgttgcttt cagcccatag cgccagctgc caaaactgcc
     1981 gtagtaacta ctggcttcaa tggttggtgg ctgttgtccg gtctgggtgg tgacattcat
     2041 taccccacca gacgcgttgc catacagggc agagaagggg ccacgcagca cttccacatt
     2101 ttgcacactg cttaaatcga tgttggatgt ttgcccttgc ccgtcgggca tggtggcggg
     2161 aataccgtcc acatacaggc gaataccgcg aataccgtaa gtggagcggg agccaaatcc
     2221 gcgaatcgac agctgtaaat cttgcgcata gttctgccgg ttttgtacct gcaaaccagg
     2281 cacgtcggtc agtgattcgg acaagttaat gcgcggtgtt gccaggcgca tctcctcgcc
     2341 atccaccacg cttactgctg ctggggtatc cagttctgaa accacctgcg gtgcggcact
     2401 gacaatcatg gtctgttcat cagcggcaaa aacaacgggg gaaagggcaa gtagtgcggg
     2461 caaaacggtc tgtcggacgg aaaaaatctt catgaaaaaa gccaggttaa gaatgggaaa
     2521 acgccgttat ggtaataaaa ttgtaaattc atggaaaacg aaacggcacg atacgttaag
     2581 taattgagaa aattgtagtc gtaacggcaa gaaatgctcc acatttgaga aaataatgat
     2641 taccattccc atttataaca agagcgtaac gatgattacg cttagcgaag cattgtgaag
     2701 cagcaaaaat atcggttcat caaagggagt cgtcatgcat ttacgtcatc tgttttcatc
     2761 gcgcctgcgt ggttcattac tgttaggttc attgcttgtt gcttcatcat tcagtacgca
     2821 ggccgcagaa gaaatgctgc gtaaagcggt aggtaaaggt gcctacgaaa tggcttatag
     2881 ccagcaagaa aacgcgctgt ggctcgccac ttcgcaaagc cgcaaactgg ataaaggcgg
     2941 cgtggtttat cgtcttgatc cggtcactct ggaagtgacg caggcgatcc ataacgatct
     3001 caagccgttt ggtgtcacca tcaataacac gactcagaca ctgtggtttg gtaacactgt
     3061 aaacagcgcg gtcacggcga tagatgccaa aacgggcgag gtgaaaggcc gtctggtgct
     3121 ggatgatcgt aagcgtacgg aagaggtgcg cccgctgcaa ccgcgtgagc tggtagctga
     3181 cgatgccacg aacaccgttt acatcagtgg tattggtaaa gagagcgtga tttgggtcgt
     3241 tgatggcgag aatatcaaac tgaaaaccgc catccagaac accggtaaaa tgagtaccgg
     3301 tctggcgctt gatagcaaag gcaaacgtct ttacaccact aacgctgacg gcgaattgat
     3361 taccatcgac accgccgaca ataaaatcct cagccgtaaa aagctgctgg atgacggcaa
     3421 agagcacttc tttatcaaca ttagccttga taccgctagg cagcgtgcat ttatcaccga
     3481 ttctaaagcc gcagaagtgt tagtggtcga tacccgtaat ggcaatattc tggcgaaggt
     3541 tgcagcaccg gaatcactgg ctgtgctgtt taacccagcg cgtaatgaag cctacgtaac
     3601 gcatcgtcag gcaggtaaag tcagtgtgat tgacgcgaaa agctataaag tagtgaaaac
     3661 gttcgatacg ccgactcatc caaacagcct ggcgctgtct gccgatggca aaacgctgta
     3721 tgtcagtgtg aaacaaaaat ccactaaaca gcaggaagct acccagccag acgatgtgat
     3781 tcgtattgcg ctgtaataga tatggcgagg gtgaaaacgg agagacttgc ctgactttta
     3841 tcacgccgca tgcggcacac cttcgtcagt aatctaaatt tgcccggcac gtagccgggc
     3901 aattttgcaa ttagctcgcc gtttcaggct taaacacaat ttcctgtttt tcttcatctt
     3961 cctcgacgac tggcgcagtg ctgtgaattt cagcaacgcg tttgcgcaca ccaaaccagc
     4021 ctataaccag cagaataccg ataatcggca gcgccgcgat agtataagtc ccgttcgggt
     4081 aatcgaacgc catcagcaca aggacactca gtaaaaacag taatgtcagc caggaagtga
     4141 agggcgcgcc aggcagttta aaactgacat ccgctgcttt gccttcttta atcgctttac
     4201 gcaggcgcat ctggcacacg atgataaacg cccatgaagc gatgattccc agcgacgcga
     4261 agttcaacac aatctcaaat acgcgcgacg gcaccagata gttgaggaat acgccgacga
     4321 catacacaac cagtgtcgcc agaatcccgg catacggcac atgctgacga ctcattttcg
     4381 ccataaaact cggtgcggaa ccgcccatcg ccatcgagcg cagaatacgc ccggtgcagt
     4441 acagacctga gttcaggctg gagagggcag cggtcagcac cacaatgttc ataatgctgc
     4501 cgatatatgg cacacccagt ttagagaaaa acgtcacgaa cggactttgc cccgcctgat
     4561 acgcgctcca cggcaataac ataaccagca acaccacgga gccgacgtaa aacaggccaa
     4621 tacgccaaat cacactgtta atggctttag gcaccatggt ctgcggatct ttacattctc
     4681 cggcagctgt acccaccatt tcaatggagg caaaagcaaa cactacgccc tgaatcaaca
     4741 ccagcgcagg cagcaaaccg tgggggaaga agccgccatt atcggtgatt aaatgaaagc
     4801 cagtggtgtt gccatccagc ggctgaccac taccgaggaa cactgtaccc acgaccaaaa
     4861 aggtcacaat ggcgagcact ttaataagcg caaaccagaa ctccatctcc gcaaaccatt
     4921 tcacaccgat catattcatg gtgccaacga tggtaagtgc agcgagcgca aagacccact
     4981 gcggcacgcc gccaaacgca ccccagtaat gcatatacag agcgacggcg gtaatatcaa
     5041 caatccccgt catcgcccag ttgatgaagt acatccagcc agcaacataa gcggctttct
     5101 cacccaaaaa ctcacgggca taagagacaa aactgccact ggaagggcgg tgtagcacca
     5161 gctcacccaa tgcacgcaga ataaaaaacg aaaacaagcc acaaattaaa taaaccagtg
     5221 ccagtgctgg acccgccatt tgcagtcggg ctcctgcacc taaaaacaag ccggtgccaa
     5281 tcgcgccgcc aatggcgatc atctgtacct ggcgattgcc catcgctttg tgatacccct
     5341 cttcgtgggc attaagccag cggcgtttcg cggcgtgttg atctgaagtg tcggtgtcgt
     5401 gtttactcat tgctctccct gattgcttta atgaaaaagt catataagtt gccatgaaca
     5461 atgtttattc atcctggcga tgatagttat caccattatt ctttatgggt ataaaactat
     5521 cggcgcagca tcctacccgt tatcgatata cgatgcaaaa catcccctta caatcctgaa
     5581 ggggattcat acaactgacg aaaaaatgac aaatactttt gctggttaac ctgtgtactg
     5641 tcctacactt aatctttaaa agattgtgag gggcatatga ttaaggtgta tggcgtaccc
     5701 ggatggggct ctacaatcag tgagctgatg ctaacactgg ctgatattcc ttatcagttc
     5761 gttgatgtca gtggttttga ccacgaggga acttcacgcg agttattgaa aaccctgaac
     5821 ccgttgtgtc agattccgac actggcgctg gaaaatgacg agattatgac ggagacagcg
     5881 gcgattgcat tgatggttct cgatcgtcgc ccggaccttg cgccaccggt tgggcgcgcc
     5941 gaacgtcagc agtttcaacg gctattggtc tggctggttg ctaatgtcta tccaacattc
     6001 actttcgccg attaccctga gcgttgggct cctgacgcac ctgaacagtt aaagaagaat
     6061 gttattgaat atcgtaaatc gctttatatc tggctgaatt cacaattgac cgctgaacct
     6121 tatgcgtttg gtgagcaact aacgctggtg gactgttatc tttgcactat gcgcacatgg
     6181 gggtctgggc atgaatggtt tcaggataat gccacgaata tcagtgcgat tgctgatgct
     6241 gtatgtcagt taccgaaatt acaagaagta ttaaaaagga atgaaattat ttgagtggga
     6301 taccagagga ttatgttatt tatta
//
LOCUS       DAZOXR010000215         6312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZOXR010000215 DAZOXR010000000
VERSION     DAZOXR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.222"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUNN_003855"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUNN_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0578105.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUNN_003856"
     CDS             2219..2767
                     /locus_tag="ACHUNN_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0578106.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUNN_003857"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUNN_003857"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0578107.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUNN_003858"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUNN_003858"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0578108.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUNN_003859"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUNN_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0578109.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1576 a         1555 c         1604 g         1577 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa ag
//
LOCUS       DAZOXR010000216         6305 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZOXR010000216 DAZOXR010000000
VERSION     DAZOXR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.223"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..277)
                     /locus_tag="ACHUNN_003860"
     CDS             complement(<1..277)
                     /locus_tag="ACHUNN_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578110.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVS"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="ACHUNN_003861"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="ACHUNN_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0578111.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="ACHUNN_003862"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="ACHUNN_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="ACHUNN_003863"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="ACHUNN_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="ACHUNN_003864"
     CDS             complement(5959..>6305)
                     /locus_tag="ACHUNN_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0578112.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1411 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAZOXR010000217         6256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZOXR010000217 DAZOXR010000000
VERSION     DAZOXR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.224"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHUNN_003865"
     CDS             122..952
                     /locus_tag="ACHUNN_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR0578113.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUNN_003866"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUNN_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0578114.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHUNN_003867"
     CDS             complement(2081..3541)
                     /locus_tag="ACHUNN_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0578115.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUNN_003868"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUNN_003868"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0578116.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHUNN_003869"
     CDS             5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHUNN_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0578117.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1619 c         1588 g         1569 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctg
//
LOCUS       DAZOXR010000218         6201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZOXR010000218 DAZOXR010000000
VERSION     DAZOXR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.225"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHUNN_003870"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHUNN_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(796..1248)
                     /locus_tag="ACHUNN_003871"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHUNN_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHUNN_003872"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHUNN_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUNN_003873"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUNN_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0578118.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHUNN_003874"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHUNN_003874"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHUNN_003875"
     CDS             complement(2927..3859)
                     /locus_tag="ACHUNN_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0578119.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUNN_003876"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUNN_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0578120.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5090..6158)
                     /locus_tag="ACHUNN_003877"
                     /pseudo
     CDS             complement(5090..6158)
                     /locus_tag="ACHUNN_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1368 c         1374 g         1913 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acctggcgca gatagttgat taatttcatt ttgatatttc gcctggcgat
     5161 tttgaagtct tttttcaacg ttatcttcga gatccttaaa gtctttgtat ttattttcag
     5221 gatggtcaac gtcaactttg ttagcgaata aattagaaat tatttctttt acattgtcgc
     5281 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     5341 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     5401 aacctgttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcat
     5461 tgctgaagca ttcctggctt tttcactaca atattgggtg agactatcac cattaatacg
     5521 aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt
     5581 tccgttttta tttttaatta caatagcgag agagttgcct tttttatctt taccgtacca
     5641 ggtaaataag tccgttggtt cgctattccc gctctcatca aattctgtta ccgtatgtct
     5701 gagtttgtgt gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa
     5761 tgcgctgaag atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt
     5821 aatgatagta cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg
     5881 gttgtcgata ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac
     5941 tgaggaatta ttaatattaa atcttaagga tcgtcgttta ctcgtatggc gttttttata
     6001 agctcaagta gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg
     6061 agctcttgta ctaatccatt aacttcatca ttgttatttt cattaatgga ttctgggtaa
     6121 gttgcgctgg tattgttgat tgaaatagat ggtagcatga ttttattcca ttttgattga
     6181 tttgttacaa atatatatta c
//
LOCUS       DAZOXR010000219         6120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZOXR010000219 DAZOXR010000000
VERSION     DAZOXR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.226"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHUNN_003878"
     CDS             <1..358
                     /locus_tag="ACHUNN_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0578121.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHUNN_003879"
     CDS             complement(398..1069)
                     /locus_tag="ACHUNN_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR0578122.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUNN_003880"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUNN_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0578123.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHUNN_003881"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHUNN_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0578124.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHUNN_003882"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHUNN_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0578125.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHUNN_003883"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHUNN_003883"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0578126.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHUNN_003884"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHUNN_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0578127.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHUNN_003885"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHUNN_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0578128.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="ACHUNN_003886"
     CDS             complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="ACHUNN_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0578129.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1497 a         1608 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
//
LOCUS       DAZOXR010000220         6118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZOXR010000220 DAZOXR010000000
VERSION     DAZOXR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.227"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            62..838
                     /gene="exuR"
                     /locus_tag="ACHUNN_003887"
     CDS             62..838
                     /gene="exuR"
                     /locus_tag="ACHUNN_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0578130.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1078..1161
                     /gene="yqiM"
                     /locus_tag="ACHUNN_003888"
     CDS             1078..1161
                     /gene="yqiM"
                     /locus_tag="ACHUNN_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0578131.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1183..1845
                     /gene="yqjA"
                     /locus_tag="ACHUNN_003889"
     CDS             1183..1845
                     /gene="yqjA"
                     /locus_tag="ACHUNN_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0578132.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1849..2232
                     /gene="mzrA"
                     /locus_tag="ACHUNN_003890"
     CDS             1849..2232
                     /gene="mzrA"
                     /locus_tag="ACHUNN_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0578133.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2379..2747
                     /gene="yqjC"
                     /locus_tag="ACHUNN_003891"
     CDS             2379..2747
                     /gene="yqjC"
                     /locus_tag="ACHUNN_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR0578134.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2785..3090
                     /locus_tag="ACHUNN_003892"
     CDS             2785..3090
                     /locus_tag="ACHUNN_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0578135.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3093..3497
                     /locus_tag="ACHUNN_003893"
     CDS             3093..3497
                     /locus_tag="ACHUNN_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0578136.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3487..3786
                     /locus_tag="ACHUNN_003894"
     CDS             3487..3786
                     /locus_tag="ACHUNN_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0578137.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3972..4364
                     /locus_tag="ACHUNN_003895"
     CDS             3972..4364
                     /locus_tag="ACHUNN_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0578138.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4434..5419
                     /gene="yqjG"
                     /locus_tag="ACHUNN_003896"
                     /pseudo
     CDS             4434..5419
                     /gene="yqjG"
                     /locus_tag="ACHUNN_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5712..6077
                     /locus_tag="ACHUNN_003897"
     CDS             5712..6077
                     /locus_tag="ACHUNN_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0578139.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1529 a         1511 c         1543 g         1535 t
ORIGIN      
        1 ggcataccca tgaataaagt ggtatgataa cttcgtccgc attccactcc gggagacgca
       61 tatggaaatc actgaaccac gccgtttgta tcaacaactt gccgctgacc tgaaagagcg
      121 cattgaacag ggcgtctatc tggtgggtga taaactgcct gccgaacgct ttattgccga
      181 tgaaaagaac gtcagccgaa cggtagttcg tgaagccatc atcatgctgg aagttgaagg
      241 ctatgtggag gtgcgtaaag gttcaggtat tcatgtggtt tccaaccagc cgcgccatca
      301 gcaggcggct gacaataata tggaattcgc caattacggt ccgtttgagt tgcttcaggc
      361 tcgccagctc atcgaaagta atattgccga gttcgcggca actcaggtaa cgaaacaaga
      421 catcatgaaa ctgatggcca ttcaggaaca ggcgcgcggc gaacaatgct tccgtgattc
      481 cgagtgggat ttacagttcc acattcaggt agccctggcg acgcagaact ccgccctggc
      541 ggccatcgtt gaaaaaatgt ggacccagcg tagccataac ccgtactgga aaaaactaca
      601 cgaacacatt gattcccgta ccgttgataa ctggtgtgat gaccacgatc aaattctcaa
      661 ggcgttaatt cgcaaagatc cccatgctgc taagctggca atgtggcagc atctggaaaa
      721 caccaaaatc atgttattta acgaaaccag cgacgatttc gagttcaatg ccgaccgcta
      781 tctattcgcc gaaaacccgg tagtgcatct cgatacggcc accagtggca gtaaatgaaa
      841 ctcctgtccg acaggcgctg cgacgcgcct gttcgcttcg tcggtaagcg aaaagtataa
      901 agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc attcagcaaa atcagactcc
      961 acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag attcaatttg
     1021 aatttatgtt tttgaatgct ttcttatctc acgatttaac agggaatagt tcaggctgtg
     1081 ttgatgtatc aaacccgcag aacataccaa aacagcaata acattgcggt agtgcatctt
     1141 ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact tttgacccaa
     1201 ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccatc gatgattggc
     1261 atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa acggcttgct tccagcggcc
     1321 tttttaccgg gcgacagttt actggtattg gtcggcgtgt tgattgcgaa aggcgcgatg
     1381 ggttacccgc aaacgattct gctgctgacc gttgccgcca gcctcggctg ctgggtcagc
     1441 tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct atctcattta
     1501 cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt atcggcgctg
     1561 ttaattggtc gctttattgc gtttgtcaga acactgctgc cgacgattgc cgggttatca
     1621 gggctgaata acgcgcgctt tcagtttttc aactggatga gcggtctgct gtgggtattg
     1681 atcctgacaa ctctgggtta catgctcggc aaaacgccga tatttttaaa gtacgaggac
     1741 cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg cctggcaggt
     1801 tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat gcaaatacct
     1861 cgcatgtcgc ttcgccagct agcctggtcc ggcactgttt tacttctggt cggcacgctg
     1921 ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg tgccgttcat
     1981 caaggcacaa cgatgccaga cggtttttcg atctggcatc accttgacgc tcatggcatt
     2041 cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacatttga ctccagcgac
     2101 cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc cccatggcta catcattgcg
     2161 cagcaggaca ataacagtca ggctatgcag tggctgaccc ggttacggga taactctcat
     2221 cgcttcggat aacttcctgt tatccgaaac atatcactca ctttggtgat ttcaccgtaa
     2281 ctgtctatga ttaatgaggc ggtggttaac actaccgctt cgttgtctgg acaatcgttc
     2341 ctttgtaata ggtccgaatc acaatggaag gttcaagaat gaaataccgc atcgctttag
     2401 ctgtttctct ctttgctctt agtgccggta gttatgccac taccctgtgt caggaaaagg
     2461 agcaaaatat ccttaaggag atcagctatg ccgaaaaaca ccaaaaccag aatcgtattg
     2521 acggtctgaa taaagccctg agtgaagtcc gggccaactg ttcagatagc cagctgcgtg
     2581 ccgatcatca caagaaaatc gcaaagcaga aagatgaggt ggcggaacgc cagcaagatt
     2641 tagccgaggc gaagcaaaaa ggcgatgccg ataagattgc caaacgcgaa cggaaactgg
     2701 cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca
     2761 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
     2821 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg
     2881 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
     2941 cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct
     3001 gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta
     3061 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
     3121 ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
     3181 aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat ctctttcaac
     3241 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
     3301 tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     3361 tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat
     3421 ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg ctcgaggagg
     3481 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     3541 cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc aacaggcgct
     3601 tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtagc
     3661 gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     3721 tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc
     3781 ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt ttgaagattt
     3841 ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca
     3901 gcaacgacgc taataccgcg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag
     3961 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgc attttaatgc
     4021 cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt acccaacaat
     4081 atatggaagc aatgggtgtc ccgggtttta tgctgccgct ggtgattctg cttgagttcg
     4141 gtggtggtct ggcaatcctg ttcggtttcc tgactcgcac cacagccctg tttactgcgg
     4201 gctttacgct gctgacggca tttttatttc acagcaactt tgctgaaggc gtcaactcgc
     4261 tgatgttcat gaaaaacctg acaatttctg gcggattcct gctgctggca attaccggtc
     4321 cgggcgcgta tagcatcgac cgcctgctga ataaaaagtg gtaagcacgc gctatactta
     4381 acgataaaaa acgaggagga agctcctcgt ttttgctatt ggaggagaga aaaatgggtc
     4441 aactgattga cggcgtctgg catgacacct ggtacgatac caaatctacc ggcggtaaat
     4501 ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgca cctggcccca
     4561 ctggcacagg cggttttatc gcagagaaag atcgttatca tctctatatt tcactcgcct
     4621 gcccgtgggc gcaccgcacg ctgatcatgc gcaaactcaa aggactggaa ccgtttattt
     4681 ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat gacagttttc
     4741 cgggagcaac cggagatacg ctctatcaac atgaatttct gtatcagctt tatctccacg
     4801 ccgatccaca ctacagcgga cgagttactg ttcccgtact gtgggacaaa aagaaccaca
     4861 ccatcgtcag caacgaatca acagaaatca tccgcatgtt caataccgcg tttgatgcgc
     4921 tgggcgcgaa agcgggtgat tactacccac cagccctgca aacgaaaatt gacgaactta
     4981 acggctggat ttatgacacc gttaacaacg gcgtgtataa agctggtttt gccaccagcc
     5041 agcaagccta cgacgaggcg gtggcgaaag tgtttgaatc gctggcgcga ctggagcaga
     5101 ttttaggcca gcatcgttac ctgaccggca accagctaac cgaagccgat attcgcctgt
     5161 ggactacgct ggtgcgtttt gatccagtgt atgtgaccca cttcaagtgt gataagcacc
     5221 gcatcagcga ttacctgaat ctgtatggct tcctgcgcga tatctaccag atgccgggga
     5281 tcgccgaaac tgtcaatttc gatcacatcc gtaatcatta cttccgcagc cataagacca
     5341 tcaaccctac ggggattatt tcaattggtc cgtggcagga tctcgatgaa ccgcaggacg
     5401 agatgttcgc ttcggttaaa aaaaggcacc ttacgaagtg cctctgataa ttagctaagt
     5461 gcatgttgtt catatcggat gcggcgtgaa cgccttatca aacctacaaa acatttatat
     5521 tcaatatgtt gcaatgaccg cgcaggccag ataagcgtag cgcatctggc gattttgcgc
     5581 ttgtcatcag tcttagcgcg ccttcacagc actttattaa ttccttgaac gaatatttac
     5641 tgccatttca ttcacgtcta ttcttaattt gctgctttaa aaacaagtga ttgagcaaca
     5701 ttgaggcaaa aatggactgg tatctgaaag taataaaaaa ttatgtcggt ttccggggtc
     5761 gtgcgcggcg caaagagtac tggatgttta ttctggtcaa catcatcttt acgttcgtgc
     5821 tggggctgct ggataaaatg ttaggctggc aacgcgctgg tggcgaaggc atcctgacga
     5881 caatctacgg tattctggtg tttttaccgt ggtgggcagt tcagttccgc cgcctgcacg
     5941 acaccgaccg ctcggcgtgg tgggcactgc tattcttaat cccgtttatc ggctggctta
     6001 ttatcatcgt ctttaactgc caggcgggta cgccaggcga aaaccgcttt ggacctgatc
     6061 caaaactgga accttaaaac aaatctgctc gccggatgtc tgactcatcc ggctttta
//
LOCUS       DAZOXR010000221         6087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZOXR010000221 DAZOXR010000000
VERSION     DAZOXR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.228"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUNN_003898"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUNN_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0578140.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUNN_003899"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUNN_003899"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0578141.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHUNN_003900"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHUNN_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0578142.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUNN_003901"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUNN_003901"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0578143.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1617 a         1609 c         1449 g         1412 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcgg
//
LOCUS       DAZOXR010000222         6015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZOXR010000222 DAZOXR010000000
VERSION     DAZOXR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.229"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(369..1304)
                     /locus_tag="ACHUNN_003902"
     CDS             complement(369..1304)
                     /locus_tag="ACHUNN_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0578144.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="ACHUNN_003903"
     CDS             complement(1802..1945)
                     /locus_tag="ACHUNN_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR0578145.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="ACHUNN_003904"
     CDS             complement(2029..2286)
                     /locus_tag="ACHUNN_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HFR0578146.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="ACHUNN_003905"
     CDS             complement(2296..3933)
                     /locus_tag="ACHUNN_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFR0578147.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="ACHUNN_003906"
     CDS             complement(4154..4687)
                     /locus_tag="ACHUNN_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578148.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="ACHUNN_003907"
     CDS             complement(4690..5616)
                     /locus_tag="ACHUNN_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0578149.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="ACHUNN_003908"
     CDS             5792..>6015
                     /locus_tag="ACHUNN_003908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578150.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAZOXR010000223         5989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZOXR010000223 DAZOXR010000000
VERSION     DAZOXR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.230"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHUNN_003909"
     tRNA            292..368
                     /locus_tag="ACHUNN_003909"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUNN_003910"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUNN_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0578151.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHUNN_003911"
     CDS             complement(1357..2247)
                     /locus_tag="ACHUNN_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0578152.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUNN_003912"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUNN_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR0578153.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5947)
                     /gene="nfrB"
                     /locus_tag="ACHUNN_003913"
                     /pseudo
     CDS             complement(5207..5947)
                     /gene="nfrB"
                     /locus_tag="ACHUNN_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1360 a         1568 c         1563 g         1498 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga tggtgtttta tgttcagata atgcccgat
//
LOCUS       DAZOXR010000224         5952 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZOXR010000224 DAZOXR010000000
VERSION     DAZOXR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.231"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="ACHUNN_003914"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="ACHUNN_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0578154.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..5547
                     /locus_tag="ACHUNN_003915"
                     /pseudo
     CDS             1552..5547
                     /locus_tag="ACHUNN_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1425 a         1507 c         1919 g         1101 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagcgcacag gggccgtggt
     2101 ggatactggg gggggctgag cgggtgccgg gtgctgagga cgtactgcca gcgccgctgc
     2161 cgccgtaccg ggtgcttacc gggatggcgg acgctgacgt accggcgtga ggccgccggt
     2221 gacctggccg gggaaatcac cgacgtgacg gacggtgccg ggcgggagtt ccgtctggtg
     2281 ctgaccacgc aggcgcagcg tgcggaagag gcccgcacct cttcgctatc ttcttctgac
     2341 agttcccgcc ctctctcagc ctcagcgttc cccgacacac tgcccggtac cgaatacggc
     2401 cccgacaggg gtatccgcct ttcggcggtg tggttgatgc acgacccggc atacccggag
     2461 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
     2521 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcgcccgggc
     2581 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacg
     2641 gggcgggtgg tggagcaact gaacccggca gggttaagct accgctatct ttatgagcag
     2701 gaccgcatca ccgtcaccga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2761 gccgggctga aacgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcgcagcggg
     2821 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
     2881 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2941 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtacccgga cgggctggag
     3001 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
     3061 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
     3121 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
     3181 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
     3241 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
     3301 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
     3361 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     3421 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
     3481 agtctgacta acgagaacgg cagtcacagc gacttcagct acgatgcgct ggaccggctg
     3541 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
     3601 ctcacgcaga gtgaggatga ggggcttgtc accctctggc actacgatgc gtcggaccgc
     3661 atcacgcacc ggacggtgaa cggcgacccg gcagagcagt ggcagtatga cggccacggc
     3721 tggctgagag aaatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
     3781 gacgataagg gccgcctgac gggcgaacgg cagacggtgg agaacccgga gacgggggaa
     3841 ctgctgtggc agcatgagac gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc
     3901 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta cctggcgggc
     3961 atgaagctgg gcgggacgcc gctggtcgag tatacgcggg acaggctgca ccgtgagacg
     4021 gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt atgccaccga cccggcggga
     4081 aaccgcctgc cggacccgga gctacacccg gacagcacgc tcacggcgtg gccggataac
     4141 cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag actgacggag
     4201 aagacggacc gcatcccgac gggtgtgata cggacggacg acgagcggac ccaccactac
     4261 gacagccagc accgcctggt gttccacacg cggatacagc atggcgagcc actggtcgag
     4321 agccgctacc tctacgaccc gctgggacgg cgaatggcga aacgggtatg gcggcgggag
     4381 cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg aggagacatg gtacggctgg
     4441 gacggcgaca ggctgacgac ggtacagacc gacaccacac gtatccagac ggtatacgag
     4501 ccgggaagct tcacgccgct catccgggtc gagacagaga acggcgagcg ggagaaagcg
     4561 cagcgccgca gcctggcgga gaagctgcag caggaaggga gcgaggacgg gcacggcgtg
     4621 gtatttccgg ctgaactggt gcggctgctg gacagactgg aggaagaaat ccgggcagac
     4681 cgcgtgagca gtgaaagccg ggtgtggctt gcgcagtgcg ggctgacggt ggagcaactg
     4741 gccagacagg tggagccgga atacacacca gtgcgaaacg ttcatcttta ccactgcgac
     4801 catcggggcc agccgctggc gctcatcagc gaagacggcg atacggtgtg gagcggggcg
     4861 tatgatgagt ggggcaacca gctaaatgag gagaacccgc atcatgtgta tcagccatac
     4921 cgtctgccgg ggcagcagta tgatgaggag tcggggctgt actacaatcg tcaccggtac
     4981 tatgatccgt tgcagagacg gtacatcacc caggacccca ttgggctgaa aggtgggtgg
     5041 aatctgtatc agtatccgct gaatccggtg agcaggatag atccgttggg gttattctca
     5101 tgtgagagtt gtagtcattt tatagatgat ataaataata aactggataa ccttccgttt
     5161 aacccgacaa aaatgtttac agctagttta aatactgcta attcagcaag gctgtatgca
     5221 agtggcatga caaaattcgg tataagtgtt gctgccgatg gaacgatcat tgctgcccca
     5281 gtcggtgcag cttctatggc tttaggggcg tggaatataa aatctgcgac agctgcatat
     5341 aacagagcaa atttacaatt atcagaatca cttcaggaaa atggtagaga taggtgttgg
     5401 agaaatttat tgggtatttt accttatggt gagcagtttg atgaccctgg tgaaccatca
     5461 atattagacg tttatggtga aaagattgag aatatcaaag attctccaat gaagtttttt
     5521 gaagagcttg ggacgctggg gttgtaatgt gtatttttaa tgatattttt atgtttgtgt
     5581 ttattgttag tagtatactt gtaaccgtgc attttgtgta ttctttattt tttaaaagtg
     5641 gaaaggtaaa agataaggta aaaaaatgga ttaaagtaat gtgtgattta ttttggggga
     5701 taggatagtg gtgtgatatg gataaatata aattacagtg gctggcatat ttcgacctga
     5761 cgccggagta gataaaaaaa cagacaggat tggagtatag caagcgtctg acaatctctc
     5821 gggacagtag tatgatgaag aggcggggtt gtactataac cgtaaccggt actacgatcc
     5881 gctaaagggc aagtatatga ctcagggaaa gatcatcaga agttcacttt ttgtactaaa
     5941 taattcgcat tt
//
LOCUS       DAZOXR010000225         5925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZOXR010000225 DAZOXR010000000
VERSION     DAZOXR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.232"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHUNN_003916"
     CDS             complement(38..400)
                     /locus_tag="ACHUNN_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0578155.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUNN_003917"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUNN_003917"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0578156.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUNN_003918"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUNN_003918"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0578157.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUNN_003919"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUNN_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0578158.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUNN_003920"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUNN_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0578159.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHUNN_003921"
     CDS             5321..5683
                     /locus_tag="ACHUNN_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0578160.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1503 a         1457 c         1527 g         1438 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgagat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gattt
//
LOCUS       DAZOXR010000226         5683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZOXR010000226 DAZOXR010000000
VERSION     DAZOXR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.233"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..819
                     /gene="yneK"
                     /locus_tag="ACHUNN_003922"
     CDS             <1..819
                     /gene="yneK"
                     /locus_tag="ACHUNN_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0578161.1"
                     /translation="ENIEFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQT
                     GVERVLFLFLNDFIEQFPMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNF
                     ISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKH
                     VLPEVCICFLFLLAEQESPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDD
                     KIIKHMARRTLELFVKNETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            969..2159
                     /gene="ydeA"
                     /locus_tag="ACHUNN_003923"
     CDS             969..2159
                     /gene="ydeA"
                     /locus_tag="ACHUNN_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0578162.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2184..2849)
                     /gene="marC"
                     /locus_tag="ACHUNN_003924"
     CDS             complement(2184..2849)
                     /gene="marC"
                     /locus_tag="ACHUNN_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0578163.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3061..3495
                     /gene="marR"
                     /locus_tag="ACHUNN_003925"
     CDS             3061..3495
                     /gene="marR"
                     /locus_tag="ACHUNN_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0578164.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3515..3898
                     /gene="marA"
                     /locus_tag="ACHUNN_003926"
     CDS             3515..3898
                     /gene="marA"
                     /locus_tag="ACHUNN_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0578165.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3930..4148
                     /gene="marB"
                     /locus_tag="ACHUNN_003927"
     CDS             3930..4148
                     /gene="marB"
                     /locus_tag="ACHUNN_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0578166.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4205..5644)
                     /locus_tag="ACHUNN_003928"
     CDS             complement(4205..5644)
                     /locus_tag="ACHUNN_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0578167.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         1489 a         1399 c         1256 g         1539 t
ORIGIN      
        1 gaaaatattg aatttcaggt agtaataaat ttaagcccta tttatttaga taattttcgc
       61 catgatggag aaattaatat tttttgcgct cccaacccaa agcctgtcac tatggggcgc
      121 gtatttcaga ccggcgtcga gcgtgtgctc tttctgtttc tgaatgattt tattgaacaa
      181 tttccaatgt tcaaccctgg cgttccgatc aaaatagcgc atacaccaca tattgaaccc
      241 ctgcctccgg atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg
      301 aattttatct ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt
      361 cacagatggt tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac
      421 agcgaattaa aagaggattt attgcaaagc ctgatggatt cattaggttc taacaaacat
      481 gtactacccg aagtctgcat ctgcttttta ttccttttag cagaacaaga atctcctcat
      541 tttcaggact tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat
      601 cccaatgaaa gtgatttgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt
      661 atcaagcata tggcgcgcag gaccctcgaa ctgtttgtaa aaaatgaaac accgccaaaa
      721 gttactcacg aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc
      781 gcaacctcca aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc
      841 tgacgcggaa cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatacat
      901 tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat
      961 aatctgttat gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc
     1021 tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg
     1081 acattgcgca aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg
     1141 catgggtagt agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc
     1201 gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat
     1261 cgtggagctt taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt
     1321 tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg
     1381 ccttgagttt aattgccacc ggtacagcac tggcgatggt cttaggttta cctctcgggc
     1441 gcattgtggg gcagtatttc ggttggcgaa tgaccttctt cgcgattggt attggggcgc
     1501 ttatcaccct tttgtgcctg attaagttac ttcccttact gcccagtgag cattccggtt
     1561 cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa
     1621 ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa
     1681 acattgcggg attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg
     1741 gcattattgg cagcgtgatt ttcggtaaac tgggtaatca gtatgcgtct gcgttggtga
     1801 gtacggcgat tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg
     1861 aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg
     1921 gtatgcaggt taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat
     1981 tctccggcat atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt
     2041 tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt gccgcgttaa
     2101 tatggtcaat cattatattt cgccgctggc cagtgacact cgaagaacag acgcaatagt
     2161 tgaaaggccc attcgggcct tttttaatgg tacgttttaa tgatttccag gatgccgtta
     2221 ataataaact gcacacccat acataccagc aggaatccca tcagacggga gatcgcttca
     2281 atgccaccct tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa
     2341 ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag
     2401 gttgaactct gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggacca
     2461 gcggtacttg gcattgctag cggcacaaag gcaatattgg cactgggttc atcttccagc
     2521 tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaagagcatc
     2581 cgaaaaccga taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg
     2641 ccaaatgtat ccatcaccag ttgcccggcg taatacgcca ccatcatgat ggcaaatacg
     2701 tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt gcctgccagg
     2761 ccaagaaata gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc
     2821 aggccaattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat
     2881 cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc
     2941 gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt cattcatttg
     3001 acttatactt gcctgggcaa tattatcctc tgcaactaat tacttgccag ggcaactaat
     3061 gtgaaaagta ccagcgatct gttcaatgaa attattccat tgggtcgctt aatccatatg
     3121 gttaatcaga agaaagatcg tctgcttaac gagtatctgt ctccgctgga tattaccgcg
     3181 gcacagttta aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg
     3241 aaaaaagtgt tgtcggtcga cctgggagca ctgacccgta tgctggatcg cctggtctgt
     3301 aaaggctggg ttgaaaggtt gccgaacccg aatgataagc gcggcgtact ggtaaaactt
     3361 accaccagcg gcgcggcaat atgcgaacaa tgccatcaat tagttggcca ggacctgcat
     3421 caagaattaa caaaaaacct gacggcggac gaagttgcaa cacttgagca tttgcttaag
     3481 aaagtcctgc cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata ctgacgctat
     3541 taccattcat agcattttgg actggatcga ggacaacctg gaatcgccac tgtcactgga
     3601 gaaagtgtca gagcgttcgg gttactccaa atggcacctg caacggatgt ttaaaaaaga
     3661 aaccggtcat tcattaggcc aatacatccg cagccgtaag atgacggaaa tcgcgcaaaa
     3721 gctgaaggaa agtaacgagc cgatactcta tctggcagaa cgatatggct tcgagtcgca
     3781 acaaactctg acccgaacct tcaaaaatta ctttgatgtt ccgccgcata aataccggat
     3841 gaccaatatg cagggcgaat cgcgcttttt acatccatta aatcattaca atagctagtt
     3901 gataacgtga caacgtcact gaggcaatca tgaaaccact ttcatccgca atagcagctg
     3961 cgcttattct cttttccgcg cagggcgttg cggaacaaac cacgcagcca gttgttactt
     4021 cttgtgccaa tgtcgtggtt gttcccccat cgcaggaaca accaccgttt gatttaaatc
     4081 acatgggtac tggcagtgat aagtcggatg cgctcggcgt gccctattat aatcaacacg
     4141 ctatgtagtt attgtcaggc tctgatcaca gagcctgaca tctccgtaat atcttcctca
     4201 agtctcaaat attttcgcca ttgttggcga tgagatcccg ataccagtaa aacgacgctt
     4261 tacgtatccg ttccagcgtt cccttccctt cgttgtcttt atcgacataa atcattccgt
     4321 agcgcttttt catttcccct gttccggcag aaaccaggtc aatgcagccc cacggggtgt
     4381 agccaattaa gtcaacacca tcttcaacta cggctttttt catttcccga atatgggaag
     4441 caaagtaatc aatgcgatag tgatcgttca ccgtgccgtc agcttgtctc tggtcaaccg
     4501 caccaaatcc attttcgaca ataaacagcg gcaactggaa atgatcccag aaccagttga
     4561 gtgaataacg tagccctgcc ggatcaattt gccatcccca ttcggatgta tcgatataag
     4621 ggttgctgac caaatctcgt ggttcaacat aatccagttg cgggttatcg ggagaaaatt
     4681 gtgtcgtaaa agacatgtag tagctaaaac cgataaagtc gacacatcca ctggcaagaa
     4741 tcgcgttatc ttctggtgtg atatcgaggt tgaatccttt cctggcaaag taattcagca
     4801 tatgttgcgg ataatatcca cgagcatgaa catcagtaaa ccagtaacga cgatgcatcg
     4861 ctttcgtggc catcatcata tcgttgggtg cacacgtcag aggatagatg gggcacatgg
     4921 cgatcataca gccaatgtta aattcaggat tgatcgattt tccagtctgt accgccaggg
     4981 cactggcaac taactcgtaa tgtaccgcct ggtacattat ttgttcgcgc tcattgatat
     5041 cttcctctgg cgaatacaag ataccggaat tagtaaatgg acacaggctt tcgctgaaat
     5101 tcacctgatt attgatttcg ttaaacgtca tccagtactt tactttttct ttatagcgcg
     5161 tgaagaccgt tgatgcgaag cggataaaaa agtcgatcag cttacggttt cgccagccac
     5221 catattttgt caccagatga taaggcatct caaaatgcga aagcgtcacc acaggttcca
     5281 ttccttgctt caggcattca tcgaacagat catcataaaa ttgtaaaccc tcttcattcg
     5341 gctcctgttc gtcaccttgc ggaaagattc gtgtccaggc aatggaagtt cgaaagcatt
     5401 tgaatcccat ctcggcaaat aactgaatat ctgttttata gcgatgataa aaatcaattg
     5461 cttcatgatt gggataatta agcccgtcga taacgccttc tgtcacttca cgcggcaccc
     5521 cgtgagcgcc agcagtcatc acatcagcga tactgatgcc ttttcctcct tcattccagc
     5581 caccttccaa ctgatgcgcg gctaccgcgc caccccataa aaaacctttt ttaaatcctg
     5641 acatacacaa ctccttaact aaaatagatt accaccagac ttc
//
LOCUS       DAZOXR010000227         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZOXR010000227 DAZOXR010000000
VERSION     DAZOXR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.234"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUNN_003929"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUNN_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0578168.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUNN_003930"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUNN_003930"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0578169.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUNN_003931"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUNN_003931"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0578170.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUNN_003932"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUNN_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0578171.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZOXR010000228         5632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZOXR010000228 DAZOXR010000000
VERSION     DAZOXR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.235"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="lpfD-O113"
                     /locus_tag="ACHUNN_003933"
     CDS             <1..741
                     /gene="lpfD-O113"
                     /locus_tag="ACHUNN_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0578172.1"
                     /translation="NLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSIS
                     SGGKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQ
                     SCEINNLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSF
                     YATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFS
                     AAPASLTGARPAPGQFTATATITVEIVR"
     gene            989..2029
                     /gene="pstS"
                     /locus_tag="ACHUNN_003934"
     CDS             989..2029
                     /gene="pstS"
                     /locus_tag="ACHUNN_003934"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0578173.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2116..3075
                     /gene="pstC"
                     /locus_tag="ACHUNN_003935"
     CDS             2116..3075
                     /gene="pstC"
                     /locus_tag="ACHUNN_003935"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0578174.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3075..3965
                     /gene="pstA"
                     /locus_tag="ACHUNN_003936"
     CDS             3075..3965
                     /gene="pstA"
                     /locus_tag="ACHUNN_003936"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0578175.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4056..4829
                     /gene="pstB"
                     /locus_tag="ACHUNN_003937"
     CDS             4056..4829
                     /gene="pstB"
                     /locus_tag="ACHUNN_003937"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0578176.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4844..5569
                     /gene="phoU"
                     /locus_tag="ACHUNN_003938"
     CDS             4844..5569
                     /gene="phoU"
                     /locus_tag="ACHUNN_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0578177.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1388 a         1352 c         1482 g         1410 t
ORIGIN      
        1 aaccttgatg ttgggattgc tatagatatt cttggggttg ggtatgtgaa tgcacctttt
       61 gaatatcatg ctaacaaccc gtctggtaat acaaagtaca attgcaaccg cattgaacct
      121 ttaagtatat ccagtggggg taaagcgata gtatattttt atatcaagaa gacatttgca
      181 ggaaaattga ttattcctga aacaaagata gtgacattgt atggaacaat tagccgtgac
      241 accccggtgg attactcaca gccgatggct gatgtttata ttcggggcga tattacagct
      301 ccgcaaagtt gcgaaataaa taatttacag ccagtttatt ttgattttaa agaaatacca
      361 gctgcagatt tttcatctgt tgttggaagt gcggtaacaa cacataaaat tactaaaaca
      421 gtcactattg agtgtgaaaa cctggggata ctgaataccg atgatatcag tacgtctttt
      481 tatgctaccg aacccaatac ggacaattca atggtcgtaa cttcaaactc taacgtgggg
      541 ataaaaattt acgacaaaaa taataaggaa atcaaagtga acggtggtga gttgccaaca
      601 gacatgggta aatcaacagt gtatggtgaa aaatcaggta gtgtaacttt ttcagctgct
      661 cctgcgagcc ttactggagc tcgcccagcc ccggggcaat ttactgcaac agcgacgata
      721 actgttgaga ttgtgcgcta ataggtctga caacgattgt tcatcacatc gttgtcatct
      781 cacccatttc tttatcactt ttaaatgcat catgacaaaa agatgacatg tgggattttc
      841 atcttcacat ttaaaatcag taagttattt gttaattcct ttctgtcata aaaatgtcat
      901 attccttaca tataactgtc acctgtttgt cctattttgc ttctcgtagc caacaaacaa
      961 tgctttatga atcctcccag gagacattat gaaagttatg cgtaccaccg tcgcaactgt
     1021 tgtcgccgcg accttatcga tgagcgcttt ctctgtgttt gcagaagcaa gcctgacagg
     1081 tgcaggtgca accttccctg cgccggtgta tgccaaatgg gctgacactt accagaaaga
     1141 aatcggtaat aaagttaact accagggtat cggttcttcc ggtggcgtaa aacagattat
     1201 cgctaatacc gttgattttg gtgcctctga ctcgccgctg tctgacgaaa aactggctca
     1261 ggaaggtctg ttccagttcc cgaccgtgat tggcggcgtg gtgctggcgg ttaacattcc
     1321 agggctgaag tctggcgaac tggtgctgga tggtaaaacc ctcggcgaca tctacctggg
     1381 caaaatcaag aagtgggatg atgaaaccat cgccaaactg aatccgggtc tgaaactgcc
     1441 ttcacaaaac attgctgtag tacgccgcgc agatggctcc gggactacct tcgtcttcac
     1501 cagctacctg gcgaaagtga acgaagagtg gaaaaacaac gttggtactg gctctaccgt
     1561 aaaatggccg atcggtctgg gcggtaaagg taacgacggt atcgccgcgt tcgttcagcg
     1621 tctgccgggt gcaattggtt atgttgaata tgcttacgcg aagcagaaca acctggcgta
     1681 caccaaactg atctccgctg atggtaaacc ggttagtccg accgaagaaa acttcgctaa
     1741 tgcagcaaaa ggtgcagact ggagcaaaac cttcgctcag gatctgacca accagaaagg
     1801 cgaagatgca tggcctatta cctctaccac gttcattctg atccacaaag atcagaagaa
     1861 accagaacaa ggcacagaag tgctgaaatt cttcgactgg gcgtacaaaa ccggggctaa
     1921 acaggcgaac gacctggatt acgccagcct gccggatagt gtagttgaac aggttcgcgc
     1981 tgcgtggaag accaatatta aagacagtag cggtaagccg ctgtactaat aaaactccag
     2041 gccgggtacg gtgttttacg ccgcatccgg cattacaaaa tgactttgta aacgcgttta
     2101 actgaagagt aacttatggc tgcaaccaag cctgctttta acccaccggg taaaaagggc
     2161 gacataattt tcagcgtgct ggtaaaactg gcggcgctga ttgtgctatt gatgttgggt
     2221 ggcattattg tctctctgat catctcctcc tggccgagca ttcagaaatt tggtctggct
     2281 ttcctatgga ccaaagagtg ggatgcaccg aacgatatct acggggcgct ggtgccgatc
     2341 tacggtacgt tggtgacttc gtttatcgcg ctgctgatcg ccgtcccggt gagtttcggt
     2401 atcgccctgt tcctgactga gcttgcgcct ggctggctga aacgcccgct gggtatcgcc
     2461 attgagctgc tggcagccat tccaagtatc gtttacggca tgtggggcct gtttatcttt
     2521 gcgccgctgt tcgccgttta ctttcaggag ccggtcggca atatcatgtc gaatatcccg
     2581 attgttggcg cgctgttctc tggccccgca tttggtatcg gtatcctcgc ggcaggcgtg
     2641 atcctcgcca tcatgattat tccgtacatt gcggcggtaa tgcgtgatgt gttcgaacaa
     2701 accccggtga tgatgaaaga gtcggcctac ggtattggct gcaccacctg ggaagttatc
     2761 tggcgtatcg ttcttccgtt caccaaaaat ggtgttatcg gcggcatcat gctggggctg
     2821 ggccgcgcgc tcggtgaaac catggcggtg acctttatca tcggtaacac ctaccagctc
     2881 gacagcgcct cgctgtacat gccgggcaat agtatcacct ctgcgttggc gaacgaattt
     2941 gcggaagcgg aatccggtct gcacgttgcc gcactgatgg aactgggcct gatcctgttt
     3001 gtgattacct tcatcgtcct cgccgcatcg aagtttatga ttatgcgcct ggctaagaat
     3061 gagggggcac gctaatggct atggttgaaa tgcaaaccac tgcggcgctg gctgaatctc
     3121 gccgcaaaat gcaggcgcgt cgccgcctca aaaaccgtat tgcgctgacg ctctcgatgg
     3181 cgacgatggc cttcggcctg ttctggctga tctggatttt aatgtccacc atcactcgcg
     3241 gtatcgacgg tatgtcgctg gcgctgttca ctgaaatgac gccgccgccc aatacggaag
     3301 gtggtggtct ggcgaacgcc ctggcgggta gcgggctgtt aattttgtgg gccacggtat
     3361 tcggtacgcc gctgggcatt atggcgggga tttatctggc ggaatatggt cgtaaatcct
     3421 ggctggcaga agtgattcgc ttcattaacg acattctgct ctccgtgccg tcgattgtgg
     3481 ttggtctgtt tgtctacacc atcgtggtgg cacagatgga gcacttctcc ggctgggcgg
     3541 gcgtaattgc gctggcgctg ttgcaggtgc cgatcgttat ccgcaccacc gagaacatgc
     3601 tgaaactggt gccggacagc ctgcgtgaag cggcttatgc gctgggtaca ccgaagtgga
     3661 agatgatctc cgcgattacg ctgaaagcgt cggtgtccgg gattatgacc ggtatcctgc
     3721 tggcgattgc ccgtattgcc ggtgaaaccg cgccgctgct gtttaccgcg ctctccaacc
     3781 agttctggag cacggacatg atgcagccga tcgccaacct gccggtgacg atctttaagt
     3841 ttgcgatgag cccgtttgcg gaatggcaac aactggcctg ggccggggta ttgatcatta
     3901 ccctgtgcgt cctgctgctg aacattctgg cgcgcgttgt ttttgcgaag aataaacacg
     3961 gttaacactt tgccggatgc ggcgtgaacg ccttacccgg cctacggtaa gcctgattag
     4021 cgaaaagcat caggtacgat gaggaaaaga ttgcaatgag tatggttgaa actgccccga
     4081 gtaaaattca ggttcgtaat ttgaacttct actacggcaa attccatgcc ctgaaaaaca
     4141 tcaacctgga tatcgctaaa aaccaggtaa cggcgtttat cgggccgtcc ggctgcggta
     4201 aatcgacgct gctgcgtacc ttcaacaaaa tgtttgaact gtatccggag cagcgcgcag
     4261 aaggtgaaat tctgcttgat ggcgacaaca tcctgaccaa ctctcaggat atcgcactgc
     4321 tgcgtgcgaa agtgggcatg gtgttccaga aaccgacgcc gttcccgatg tccatctacg
     4381 acaacatcgc ttttggtgtt cgtctgtttg agaagctctc ccgtgccgac atggacgagc
     4441 gcgtgcagtg ggcattgacc aaagccgcat tgtggaacga aaccaaagat aaattgcacc
     4501 agagcggtta ctctctctct ggtggtcagc aacagcgtct gtgtattgcg cgtggtatcg
     4561 ccattcgccc ggaagtgctg ctgctcgacg aaccgtgttc ggcgctcgac cccatctcta
     4621 ccgggcgtat tgaagagctg atcaccgaac tgaagcagga ttacaccgtg gtgatcgtta
     4681 cccacaacat gcagcaggct gcgcgttgtt ccgaccacac ggcgtttatg tacctgggcg
     4741 aattgattga gttcagcaac acggacgatc tgttcaccaa gccagcgaag aaacaaacag
     4801 aagactacat caccggtcgt tacggttaat tcaggagtgc gttatggaca gtctcaatct
     4861 taataaacat atttccggcc agttcaacgc cgaactggaa agtatccgca cgcaggtgat
     4921 gaccatgggt ggcatggtgg agcagcagct ttctgatgca atcaccgcga tgcataacca
     4981 ggacagcgat ctggcgaagc gcgtgatcga aggcgacaag aacgtcaaca tgatggaagt
     5041 ggcgatcgat gaagcctgcg tgcgcattat cgccaaacgt cagccgacgg cgagcgacct
     5101 gcgactggtt atggtgatca gtaaaaccat tgccgagctg gagcgtattg gcgacgtggc
     5161 ggacaaaatc tgccgtactg cgctggagaa attctcccag cagcatcagc cgttgctggt
     5221 aagtctggag tcgctgggcc gtcataccat ccagatgctg cacgacgtgc tggacgcgtt
     5281 cgcgcggatg gacattgacg aagcggtacg tatttatcgt gaagataaaa aagtcgatca
     5341 ggaatacgaa ggtattgttc gtcaactgat gacctacatg atggaagatt cgcgtaccat
     5401 tccgagcgta cttactgcgc tgttctgcgc gcgttctatc gaacgtattg gcgaccgctg
     5461 ccagaatatt tgtgagttta tcttctacta cgtgaagggg caggatttcc gtcatgtagg
     5521 cggtgatgag ctggataaac tgctggcggg aaaagatagc gacaaataat tcaccagaca
     5581 aatcccaata acttaattat tgggatttgt tatatataac tttataaatt cc
//
LOCUS       DAZOXR010000229         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZOXR010000229 DAZOXR010000000
VERSION     DAZOXR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.236"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHUNN_003939"
     CDS             complement(<1..513)
                     /locus_tag="ACHUNN_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0578178.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHUNN_003940"
     CDS             complement(759..1211)
                     /locus_tag="ACHUNN_003940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0578179.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUNN_003941"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUNN_003941"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0578180.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUNN_003942"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUNN_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0578181.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHUNN_003943"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHUNN_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUNN_003944"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUNN_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0578182.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHUNN_003945"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHUNN_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0578183.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZOXR010000230         5532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZOXR010000230 DAZOXR010000000
VERSION     DAZOXR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.237"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHUNN_003946"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHUNN_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0578184.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHUNN_003947"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHUNN_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR0578185.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHUNN_003948"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHUNN_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0578186.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUNN_003949"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUNN_003949"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0578187.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUNN_003950"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUNN_003950"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0578188.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHUNN_003951"
     CDS             4447..4812
                     /locus_tag="ACHUNN_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0578189.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHUNN_003952"
     CDS             4962..5480
                     /locus_tag="ACHUNN_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0578190.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5470..>5532
                     /locus_tag="ACHUNN_003953"
     CDS             5470..>5532
                     /locus_tag="ACHUNN_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578191.1"
                     /translation="MMDNDNSLNKRPTFKRALRNI"
BASE COUNT         1522 a         1393 c         1240 g         1377 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgcaaca tc
//
LOCUS       DAZOXR010000231         5440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZOXR010000231 DAZOXR010000000
VERSION     DAZOXR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.238"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(197..292)
                     /locus_tag="ACHUNN_003954"
                     /pseudo
     CDS             complement(197..292)
                     /locus_tag="ACHUNN_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(289..855)
                     /locus_tag="ACHUNN_003955"
     CDS             complement(289..855)
                     /locus_tag="ACHUNN_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0578192.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            1165..2937
                     /gene="aspS"
                     /locus_tag="ACHUNN_003956"
     CDS             1165..2937
                     /gene="aspS"
                     /locus_tag="ACHUNN_003956"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0578193.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            3055..3507
                     /gene="nudB"
                     /locus_tag="ACHUNN_003957"
     CDS             3055..3507
                     /gene="nudB"
                     /locus_tag="ACHUNN_003957"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0578194.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3536..4276
                     /locus_tag="ACHUNN_003958"
     CDS             3536..4276
                     /locus_tag="ACHUNN_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0578195.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4311..4832
                     /gene="ruvC"
                     /locus_tag="ACHUNN_003959"
     CDS             4311..4832
                     /gene="ruvC"
                     /locus_tag="ACHUNN_003959"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0578196.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4834..5436)
                     /locus_tag="ACHUNN_003960"
     CDS             complement(4834..5436)
                     /locus_tag="ACHUNN_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0578197.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1364 a         1316 c         1480 g         1280 t
ORIGIN      
        1 agtgagggaa agtgattttt tggggcatgc atggggcaaa aatatttgtt gtggggcatt
       61 ttggggcatg attgggacac tttgttacat atgaactttg ccgattttca tatggcatgg
      121 caatccaacg ctttgattat tctaaagaaa acacatgctc ttgggcgttc tttagtgatt
      181 tttaaaaatc ccgtatcacg cagttaaagt ggcgggcata ctcttcaagg ctggtgatcc
      241 ccaaccgcac ccatttagga tgcgaccatt gcggtagacc gatgtaaatc ataacgcgtg
      301 aaggatctct tccacgctac gcacacgggc gatgcgcggg tagatatgat taatgctgtt
      361 attgtgttgc tcggcgctgg cggcactaca ggcgtcttcg gcaatcacca gattaaagcc
      421 aagttcccag gcattgcggg cggtggattc aacaccgata ttggtcgaga tcccacataa
      481 cactattgta tcgatacctc ggcggcgcag ttgcaactcc agatccgtac cgtaaaacgc
      541 accccactga cgtttgatga tttcgatatc actgtcagtt gcacctaatg cagctggatg
      601 ttgccaccag ttttcgggca acactttagc gggggagggg gcatcaaccg gctgttttaa
      661 tgcttcggcg taatcggcag accagccaac tcgcaccaga aacacgggct gaccgctggc
      721 gcgaaatttc gccgccagct tcccggcgcg attaactacc tcatcggcag tatgtggacc
      781 accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc accaacgcag tggttttagc
      841 attaagttca agcatgctga ctcctgaaaa aacaaaatcg tgcctcacac cttaatgcga
      901 ttagccgatg gcggaacgac aaattttgtt aatttttatg agttcgggca acacctttat
      961 agcaaactgg cgtagagtct gatttcaggc ccgatataaa gtggtaacga ggcgttacag
     1021 cgcgggccag gctcttacgg tgcggcagaa tttccagtat aatagccgcc ttttttcatt
     1081 cagttgtgac atacagctaa cgctgcgact tggtcacctg cgcagcaggg ggcatccgcc
     1141 ggcagctaag ttaagggata tctcatgcgt acagaatatt gtggacagct ccgtttgtcc
     1201 cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc gtcgtcgtga tcttggtagc
     1261 ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc aggtattttt cgatccggat
     1321 cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg agttctgcat tcaggttacg
     1381 ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg atatggcgac cggcgaaatt
     1441 gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag atgttctgcc gcttgactct
     1501 aaccacgtca acaccgaaga agcgcgtctg aaataccgct acctcgacct gcgtcgcccg
     1561 gaaatggcgc agcgcctgaa aacccgcgct aaaatcacca gcctggtgcg ccgttttatg
     1621 gatgaccacg gcttcctcga catcgaaact ccgatgctga ccaaagccac gccggaaggc
     1681 gcgcgtgact acttggtgcc ttctcgtgtg cacaaaggta aattctacgc actgccgcaa
     1741 tccccacagt tgttcaaaca gctgctgatg atgtccggtt ttgaccgtta ctatcagatc
     1801 gttaaatgct tccgtgacga agacctgcgc gctgatcgtc agcctgaatt tactcagatc
     1861 gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg aagtgatgga agcgctggtg
     1921 cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg atttcccggt aatgaccttt
     1981 gcggaagcag aacgccgtta tggttctgat aaaccggatc tgcgtaaccc gatggaactg
     2041 accgacgttg ccgatctgct gaaatctgtt gagtttgcag tatttgcggg gccggcgaac
     2101 gatccgaaag gccgtgttgc cgctctacgt gttccgggtg gcgcatcgct gacccgtaag
     2161 cagatcgacg aatacggtaa cttcgttaaa atctacggcg cgaaaggtct ggcttacatc
     2221 aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca gcccggtagc gaagttcctt
     2281 aatgcagaaa tcatcgaagc catcctggat cgtactgccg cgcaagatgg cgatatgatt
     2341 ttcttcggtg ccgacaacaa gaaaattgtt gccgacgcga tgggcgcact gcgcctgaaa
     2401 gtgggtaaag accttggtct gaccgacgaa agcaaatggg caccgctgtg ggttatcgac
     2461 ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg caatgcacca tccgttcacc
     2521 tcaccgaaag acatgactgc tgcagaactg aaagctgcac cggaaaatgc ggtggcgaac
     2581 gcttacgata tggtcatcaa tggttacgaa gtgggcggtg gttcagtacg tatccataat
     2641 ggtgatatgc agcagacggt gtttggtatt ctgggtatca acgaagagga acagcgcgag
     2701 aaattcggct tcctgctcga cgccctgaaa tacggtactc cgccgcacgc aggtctggca
     2761 ttcggtcttg accgtctgac catgctgctg actggcaccg acaatatccg tgacgttatc
     2821 gccttcccga aaaccacggc ggcagcgtgt ctgatgactg aagcaccgag ctttgctaac
     2881 ccgactgtac tggctgagct gagcattcag gttgtgaaga aggctgagaa taactgatat
     2941 gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg caactgagtg aactcccatg
     3001 agcatagata actatgtgaa tgggatgagc gaaggcagtc aacgaagagg cagcgtgaag
     3061 gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca tctatgcaca agatacgaaa
     3121 cgggtgctga tgttgcagcg gcgtgacgat cccgatttct ggcagtcggt aaccggcagc
     3181 gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg aagtaaagga agaggtcacc
     3241 attgatgttg tcgctgaaca actgacctta attgactgtc agcgcacggt agagtttgaa
     3301 attttttcac atttacgtca tcgctatgcg ccgggcgtga cgcgtaatac ggaatcatgg
     3361 ttctgtcttg cgctcccgca cgaacgccag gtagtcttca ctgaacatct ggcttacaag
     3421 tggcttgatg cgcctgctgc cgcggcgctc actaagtcct ggagcaaccg gcaggcgatt
     3481 gaacagtttg taattaacgc tgcctgaaca ggcagggcta tctggagata tttttatggc
     3541 aggtcatagt aaatgggcca acaccagaca tcgtaaagct gcgcaggatg ctaagcgcgg
     3601 taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg gctaagctgg gcggtggcga
     3661 tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa gcactgtcta acaacatgac
     3721 ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc ggtgatgatg atgcaaacat
     3781 ggaaaccatc atctacgaag gttacggtcc tggcggcacg gcaatcatga ttgaatgtct
     3841 gtctgacaac cgcaaccgta ccgttgctga agtgcgtcat gcatttagca aatgtggcgg
     3901 taacctcggt actgatggtt ccgttgccta tctgttcagc aaaaaaggcg tgatctcctt
     3961 cgagaaaggc gatgaagaca ccattatgga agcagcactg gaagcaggtg ctgaagacgt
     4021 tgtgacctat gatgacggcg cgattgacgt ctacactgca tgggaagaaa tgggtaaagt
     4081 gcgcgacgct ctggaagcgg caggtctgaa agcagacagc gcggaagttt ccatgatccc
     4141 gtccaccaaa gctgatatgg atgcagaaac cgcaccgaaa ctgatgcgtc tgatcgatat
     4201 gctggaagat tgcgacgacg tgcaggaagt ctaccataac ggtgaaatct ctgatgaggt
     4261 tgcagcaact ctctgatggg gcctgctaaa cagcaaaacg gagacgcgtg atggctatta
     4321 ttctcggcat tgacccgggt tcgcgcgtga tcggctatgg tgtcatccgc caggtaggca
     4381 ggcaactgtc ctacctgggt agcggatgca tacgcaccaa agtggatgat ttaccgtctc
     4441 gtctgaagct catctatgcg ggcgtgacgg aaatcatcac ccagttccag ccagattatt
     4501 tcgccattga acaagtcttt atggccaaaa atgccgactc ggctctgaaa ctggggcagg
     4561 cgcgcggcgt ggcgattgtg gcggcggtga atcaggaatt gccggtattt gaatacgcgg
     4621 cacgtcaggt aaagcaaacg gtggtaggta ttggtagtgc cgaaaaaagc caggtgcagc
     4681 atatggtccg caccttgctg aaactgcccg ctaatccaca ggcggatgcc gccgatgcgt
     4741 tagctatcgc gattacccat tgtcacgtca gccagaatgc aatgcagatg agcgaatcgc
     4801 ggctgaacct ggcgagaggg cgactgcgtt aagttatacc gcctcggtca gttcaggctg
     4861 aggcgtttca actccctccg cgtgaatcaa caccagaccc ggatgatgcc acaaactggc
     4921 tggcgtgacg gttttacgct cccacggaat agaacctaag aaccagaatt tccagaaagt
     4981 gagttttgca ttcggattgc tatttaacag ttctccaaac gtttctattt cacccaccgg
     5041 aatgcatagc gcttctttat aaatatcaaa aacaaatttt gaacagaact ggcgcgaaga
     5101 ttcgtattta aaaccggtgt ggtaaagttt gcgtagccgg gagggaacct gttcaacaat
     5161 tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc tttatagcat agcgttgatt
     5221 agacgaacgt ttaataaaac gagatagcgt ggtgatggtt gaaaggggaa cccggctttc
     5281 tgcaaccagg aagtcttcac cgttatgacc gataatgatc ccgacgtgat tactccagca
     5341 atttgatgca gctgatattt gaccaaataa ggcagcacct atacatgtaa agacgatatc
     5401 tccaatttca tattcagcag ggtaattaat attcacgttg
//
LOCUS       DAZOXR010000232         5350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZOXR010000232 DAZOXR010000000
VERSION     DAZOXR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.239"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(149..292)
                     /gene="ypaB"
                     /locus_tag="ACHUNN_003961"
     CDS             complement(149..292)
                     /gene="ypaB"
                     /locus_tag="ACHUNN_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0578198.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            420..2705
                     /gene="nrdA"
                     /locus_tag="ACHUNN_003962"
     CDS             420..2705
                     /gene="nrdA"
                     /locus_tag="ACHUNN_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0578199.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2939..4069
                     /gene="nrdB"
                     /locus_tag="ACHUNN_003963"
     CDS             2939..4069
                     /gene="nrdB"
                     /locus_tag="ACHUNN_003963"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0578200.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4069..4323
                     /gene="yfaE"
                     /locus_tag="ACHUNN_003964"
     CDS             4069..4323
                     /gene="yfaE"
                     /locus_tag="ACHUNN_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0578201.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4377..5026)
                     /gene="inaA"
                     /locus_tag="ACHUNN_003965"
                     /pseudo
     CDS             complement(4377..5026)
                     /gene="inaA"
                     /locus_tag="ACHUNN_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(5106..5210)
                     /locus_tag="ACHUNN_003966"
                     /pseudo
     CDS             complement(5106..5210)
                     /locus_tag="ACHUNN_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5205..5350
                     /locus_tag="ACHUNN_003967"
                     /pseudo
     CDS             5205..5350
                     /locus_tag="ACHUNN_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1331 a         1430 c         1302 g         1287 t
ORIGIN      
        1 acatcaacaa tcgcttacat tcgcttatat attgaccaca actgatacat cagattatgt
       61 gatgactcgt gcttagatca atttttgcaa tcattaacaa aaagattaat aaaccatcta
      121 tatcaattta tccaacctat tctgccgttc aagaaatcgc cgaacagtta tttttaacaa
      181 atttttctct tcccattgac tttcccggac accttgtctg acctaaggtg cgcgaaagcc
      241 actttttcct tcctgagtta tccacaaagt tatgcacttg caagagggtc attttcacac
      301 tatcttgcag tgaatcccaa acataccccc tatatatagt gttctaagca gcttccctta
      361 ctacaggtag tctgcatgaa actattgcgg aaagaattcc aaaaacaggt acgacataca
      421 tgaatcagaa tctgctggtg acaaagcgcg acggtagcac agagcgcatc aatctcgaca
      481 aaatccatcg cgttctggat tgggcggcag aaggactgca taacgtttcg atttcccagg
      541 tcgagctgcg ctcccacatt cagttttatg acggtatcaa gacctctgac atccacgaaa
      601 ccattatcaa ggctgccgca gacctgatct cccgtgatgc gccggattat cagtatctcg
      661 ccgcgcgcct ggcgatcttc cacctgcgta aaaaagccta cggccagttt gagccgcctg
      721 cgctgtacga ccacgtggtg aaaatggtcg agatgggcaa atacgataat catctgctgg
      781 aagactacac ggaagaagag ttcaagcaga tggacacgtt tatcgatcac gaccgtgata
      841 tgaccttctc ttatgctgcc gttaagcagc tggaaggcaa atatctggta cagaaccgcg
      901 tgaccggcga aatctatgag agcgcccagt tcctttatat tctggttgcc gcgtgcctgt
      961 tctcgaacta cccgcgtgaa acgcgcctgc aatatgtgaa gcgtttttac gacgcggttt
     1021 ccacatttaa aatttcgctg ccgacgccaa tcatgtccgg cgtgcgtacc ccgactcgtc
     1081 agttcagctc ctgcgtactg atcgagtgcg gtgacagcct ggattccatc aacgccacct
     1141 ccagcgcgat tgttaaatac gtttcccagc gtgccgggat cggcatcaac gccgggcgta
     1201 ttcgtgcgct gggtagcccg attcgcggtg gtgaagcgtt ccataccggc tgcattccgt
     1261 tctacaaaca tttccagaca gcggtgaaat cctgctctca gggcggtgtg cgcggcggtg
     1321 cggcaacgct gttctacccg atgtggcatc tggaagtgga aagcctgctg gtgttgaaaa
     1381 acaaccgtgg tgtggaaggc aaccgcgtgc gtcatatgga ctacggggta caaatcaaca
     1441 aactgatgta tacccgtctg ctgaaaggtg aagatatcac cctgttcagc ccgtccgacg
     1501 taccggggct gtacgacgcg ttcttcgccg atcaggaaga gtttgaacgt ctgtatacca
     1561 aatatgagaa agacgacagc atccgcaagc agcgtgtgaa agccgttgag ctgttctcgc
     1621 tgatgatgca ggaacgtgcg tctaccggtc gtatctatat tcagaacgtt gaccactgca
     1681 atacccatag cccgtttgat ccggccatcg cgccagtgcg tcagtctaac ctgtgcctgg
     1741 agatagccct gccgaccaaa ccgctgaacg acgtcaacga cgagaacggt gaaatcgcgc
     1801 tgtgtacgct gtctgctttc aacctgggcg caattaataa cctggatgaa ctggaagagc
     1861 tggcaattct ggcggttcgt gcacttgacg cgctgctgga ttatcaggat tacccgatcc
     1921 cggccgccaa acgtggagcg atgggtcgtc gtacgctggg tattggtgtg atcaacttcg
     1981 cttactacct ggcgaagcac ggtaaacgct actccgacgg cagcgccaac aacctgacgc
     2041 ataaaacctt cgaagccatt cagtattacc tgctgaaagc ctctaatgag ctggcgaaag
     2101 agcaaggcgc gtgcccgtgg tttaacgaaa ccacttacgc gaaagggatc ctgccgatcg
     2161 atacctataa gaaagatctg gataccatcg ctaatgagcc gctgcattac gactgggaag
     2221 cattgcgtga atcaatcaaa acgcacggcc tgcgtaactc tacgctttct gccctgatgc
     2281 cgtccgagac ctcttcgcag atctctaacg ccactaacgg tattgaaccg ccgcgcggtt
     2341 acgtcagcat caaagcgtcg aaagacggta ttttgcgcca ggtggtgccg gactacgagc
     2401 acctgcacga cgcctatgag ctgctgtggg aaatgccggg taacgatggc tacctgcaac
     2461 tggtaggtat catgcagaaa tttatcgatc agtcgatctc tgctaacacc aactacgatc
     2521 cgtcacgttt cccgtcagga aaagtgccga tgcagcagtt gctgaaagac ctgctcaccg
     2581 cctacaaatt cggggtcaaa acgctgtatt atcagaacac ccgtgacggc gctgaagacg
     2641 cgcaggacga tctggtaccg tcaatccagg acgatggctg cgaaagcggc gcatgtaaga
     2701 tctgatattg agatgccgga tgcggcgtaa acgccttatc cggcctacgg ctcggtttgt
     2761 aggcctgata agacgcgcca gcgtcgcatc aggctccggg tgccggatgc agcgtgaacg
     2821 ccttatccgg cctacggctc ggatttgtag gcctgataag acgcgccagc gtcgcatcag
     2881 gcacaggatg cggcgtaaaa tgccttatcc ggcattaaac tcccaacagg acacactcat
     2941 ggcatatacc accttttcac agacgaaaaa tgatcagctc aaagaaccga tgttctttgg
     3001 tcagccggtc aacgtggctc gctacgatca gcaaaaatat gacatcttcg aaaagctgat
     3061 cgaaaagcag ctctctttct tctggcgtcc ggaagaagtt gacgtctccc gcgaccgtat
     3121 agattaccag gcgctgccgg agcacgaaaa acacatcttt atcagcaacc tgaaatatca
     3181 gacgctgctg gattccattc agggtcgtag cccgaacgtg gcgctattgc cgcttatttc
     3241 tattccggaa ctggaaacct gggtcgaaac ctgggcgttc tcagaaacga ttcattcccg
     3301 ttcctatact catatcattc gtaatatcgt taacgatccg tctgttgtgt ttgacgatat
     3361 cgtcaccaac gagcagatcc agaaacgtgc ggaagggatc tccagctatt acgatgaact
     3421 gatcgaaatg accagctact ggcaccttct gggcgaaggc acccacaccg ttaacggtaa
     3481 aaccgtaacc gttagcctgc gcgagctgaa gaaaaaactg tatctctgcc tgatgagcgt
     3541 taacgcgctg gaagcgatcc gcttctacgt cagctttgct tgttcctttg cctttgcaga
     3601 acgcgaactg atggaaggta acgccaagat tattcgcctg attgcccgcg atgaagccct
     3661 gcacctgacc ggtactcagc atatgctgaa tctgctgcgc agcggcgcgg atgatccgga
     3721 gatggcggaa attgccgaag aatgtaagca ggagtgctat gacctgtttg tccaggcggc
     3781 acagcaagag aaagactggg cggattatct gttccgtgac ggttcgatga ttggtctgaa
     3841 taaagatatt ctctgccagt acgttgaata catcaccaat atccgcatgc aggcagtcgg
     3901 tttggatctg ccgttccaga cgcgctccaa cccgatcccg tggatcaaca cctggctggt
     3961 gtctgataac gtgcaggttg ctccgcagga agtggaagtc agttcttatc tggtcgggca
     4021 gattgacgcg gaagtggaca ccgacgattt gagtaacttc cagctctgat ggcccgcgtt
     4081 accctgcgca tcactggcac acaactgctg tgccaggatg aacacccttc ccttctggcg
     4141 gcgctggaat cccacaatgt tgcggttgag taccagtgcc gcgaaggtta ctgcggctcc
     4201 tgtcgcaccc gactggtcgc aggtcaggtg gactggattg ccgaaccgtt agcctttatt
     4261 cagccggggg aaattttgcc ctgttgttgc cgggcaaaag gcgatattga aatcgagatg
     4321 tgaattggtt gtagtgccag atacaacgct tatacgtctt atctggccta caacgattac
     4381 atggcgtagt aatacgcttt cacctgctcc cagtccgctt tggggattgg ctccagatat
     4441 tttccagctg gcggaagtca tgattaatcg ctttattgcg acgcaagcgg cgacgacttt
     4501 tctccaggtc aagaaaacca gcttctgctt taccttctgt tttcacatag atatggcgaa
     4561 cataacaaca gccatgctga cgattaacgc tatgcatttt cttaaacgcc agcgccactg
     4621 ctttcaacat ggcttgccgt acttcgtcag aatagggcga tactgcatgc cgggcatacc
     4681 agtcagcaat gctgatgaac cccgccatat cttcagtcac cagcaacgcg cgccattcac
     4741 cctcaatttt caccgcttca ccaaaaacga tcttcggcac gatgacaccg gcccgttcca
     4801 gttctttaat taccgcaacc tcacggacaa tcgttggtcg gccgaacgga taacgtacgg
     4861 aatgaaacag atgatgcgtc atgcgcttta catacagctt tttgccgttg cgctcgacgc
     4921 attgcacccc gctcatacca ttacggcgat agttaggctc ttcaacccag tcgccctctg
     4981 ttgcccacca gtgattaaat tcgtcgtact ttgctgaaac tgccataccc tatcgcctgt
     5041 catttttatt aacgacaatg actataggtg gttacctgag gaaaatctta atgaaacgtg
     5101 tcgtattaat gaatttcttt tgccgctact agtgtgacac cgcttttgcc cttcatttgc
     5161 agcgcataga ttccaccagc cacgaccact aatagtgcgc ccagcagata aaatgtgaat
     5221 ttaatcaggt ggatacagcg atgaatttta ttcgacaggg attaggcatc gccttattac
     5281 cagagttaac gctgaaaacc attgcaggtg aattgtgttc cgttcctcac gaaccaacct
     5341 tctatcgaca
//
LOCUS       DAZOXR010000233         5344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZOXR010000233 DAZOXR010000000
VERSION     DAZOXR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.240"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUNN_003968"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUNN_003968"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0578202.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUNN_003969"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUNN_003969"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0578203.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUNN_003970"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUNN_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR0578204.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUNN_003971"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUNN_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0578205.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1366 a         1380 c         1266 g         1332 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttt
//
LOCUS       DAZOXR010000234         5342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZOXR010000234 DAZOXR010000000
VERSION     DAZOXR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.241"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(105..173)
                     /locus_tag="ACHUNN_003972"
                     /pseudo
     CDS             complement(105..173)
                     /locus_tag="ACHUNN_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            542..1069
                     /locus_tag="ACHUNN_003973"
     CDS             542..1069
                     /locus_tag="ACHUNN_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0578206.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGVKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            1127..1360
                     /locus_tag="ACHUNN_003974"
     CDS             1127..1360
                     /locus_tag="ACHUNN_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR0578207.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGALVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            1419..3377
                     /locus_tag="ACHUNN_003975"
     CDS             1419..3377
                     /locus_tag="ACHUNN_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0578208.1"
                     /translation="MPVTKCEPETTRKASRKSAKAQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVVGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDSRAYRNLPEPEAVLTEAEE
                     ERLNELMMRYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRTGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            3432..3866
                     /gene="psiB"
                     /locus_tag="ACHUNN_003976"
     CDS             3432..3866
                     /gene="psiB"
                     /locus_tag="ACHUNN_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0578209.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3863..4582
                     /locus_tag="ACHUNN_003977"
     CDS             3863..4582
                     /locus_tag="ACHUNN_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR0578210.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQIRALEQWMIPNKLENAI"
     gene            4579..5175
                     /locus_tag="ACHUNN_003978"
     CDS             4579..5175
                     /locus_tag="ACHUNN_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578211.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSALPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
BASE COUNT         1169 a         1441 c         1656 g         1076 t
ORIGIN      
        1 tccggtcgtc aggcccgact ccgacaggca gctgctggcg cacccgccag tctccgccga
       61 acccgctggc gcggtttcgg gctgtcgcta cggtccgatg cgtgaaaacc cgggcttgca
      121 gcgaccgttc cggtcgcgcc gcccctgtcg cgccgtgacc tgcgctccct gcacacctga
      181 cggtgtgtga ggaaactgcg cctgatggcg cagcagtctt ccctcctgcc gccacctctg
      241 cggcctgttt cccgctgtgg actttccggg gggcagacgt aaccccctcc cgttccatct
      301 gtccagggtg agatgcaccg gccacaaaat ttttagccat gaacccggca aaaaattttg
      361 cgtccgcccc cggacagctg tcccgtgagg cggtaaacgg gcccccatcg gaaaggtcca
      421 ctgcggggag aaacccctgt gaagtggacg gcaggaggag ctttcgctcc tctgaagggg
      481 cacatgaacg tcccgtcgcc ggtatccggt ttatctcaca tctttcagtc aggaggttct
      541 tatgagtgca cgtggtatca acaaggtcat cctcgtcggg cgtctgggca atgatccgga
      601 agtccgttac atccccaacg ggggcgcagt ggcaaacctg caggtggcta cgtcagaaag
      661 ctggcgcgac aaacagacgg gggagatgcg ggagcagacg gaatggcacc gcgtggtgct
      721 gttcggcaag ctcgcggaag tggctggtga atatctgcgc aagggcgcgc aggtctacat
      781 cgaggggcaa ctccgtaccc gtagctggga agataacggt atcacccgtt acgtcactga
      841 aattcttgtt aagaccacgg gcaccatgca gatgctgggg agtgcaccac agcagaacgc
      901 tcaggcgcaa ccgaagcctc agcagaatgg gcagccacag agtgctgacg cgacgaaaaa
      961 aggtggcgtg aaaacgaaag gccgtggacg taaggccgcg cagcccgagc ctcagccgca
     1021 accgccggag ggggaggatt acgggttttc agacgatatc ccgttctgac tggcctgact
     1081 gtgataaccg ccccgccctg cgcggggcat caccggagat atgaggatga gcgaatattt
     1141 cagaatactt caggggctgc ctgacggctc ctttacccgc gaacaggcgg aagccgttgc
     1201 cgcacagtac cggaacgtct ttatcgagga tgaccacggg gaacattttc gcctggttgt
     1261 ccgcaataac ggtgccctgg tctggcgtac ctggaatttt gaggacggtg ccgggtactg
     1321 gatgaatcat gccatcaggg atttcgggat tcttaagtaa gagaggtgcc ggacgcgcga
     1381 acgtccggca ggacataagc aattataagg ggatgattat gcctgtaacg aagtgtgaac
     1441 cagaaaccac ccgcaaagca agccgtaaat ctgcaaaagc gcaggaaaca gtcctgtctg
     1501 ccctgctggc gcagacggag gaagtgagcg tgccgctggc ctcgctgatt aagtcaccgc
     1561 tgaatgtgcg cacggtgccg tattctgcgg agtccgtcag cgaactggca gagtccatta
     1621 agggcgtcgg cctgctgcag aatctggttg ttcatgccct gccaggtgac cgttacggtg
     1681 tcgccgcagg cggtcgccga ctggcagcac tcaacatgct ggcagagcgc aacatccttc
     1741 cggctgactg gcctgtccgc gtgaaggtta ttccgcagga actggcgact gcagcatcaa
     1801 tgactgaaaa cggtcatcgt cgggatatgc accctgccga acagattgcc ggattccgcg
     1861 caatggcgca ggaaggcaaa acacctgcac aaatcggtga tttgctgggc tattcacccc
     1921 gccatgtcca gcgaatgctg aaactggctg accttgcgcc tgtcatcctc gatgcgctgg
     1981 cagaagaccg catcaccacc gaacactgtc aggcgctggc gctggagaac gacaccgcgc
     2041 gtcaggtgca ggtgtttgaa gccgcctgtc agtcgggatg gggaggtaaa ccggaagtac
     2101 agaccattcg tcgtctggtg accgaaagtg aagtggcggt ggtggggaac actaaattcc
     2161 gcttcgtggg ggctgatgcc ttctcgccag atgaactgcg caccgatttg ttcagcgacg
     2221 acgagggtgg ctatgtggac tgtgttgcgc tcgatgctgc cctgctggaa aaactccagg
     2281 ctgttgctga acaccttcgg gaagccgaag gctgggaatg gtgcgccgga cgcatggagc
     2341 ctgtcggtga gtgccgtgaa gattcccggg cataccgcaa cctgccggag ccggaagcgg
     2401 tgctgacgga ggcagaagaa gaacgcctga acgaactgat gatgcgttac gacgcgctgg
     2461 aaaaccagtg tgaggaatct gacctgctgg cagcagaaat gaagctgatt gactgcatgg
     2521 cgaaggtcag ggcgtggacg ccggagatgc gtaccggaag cggtgtggtg gtgtcctggc
     2581 gttacggtaa tgtgtgtgtc cagcgtggtg tgcagttgcg cagtgaagat gacgcggctg
     2641 acgacgctga ccgcacggaa caggtgcagg agaaagcatc agtggaggaa atcagtctgc
     2701 cactgctgac gaaaatgtct tccgaacgca cgctggcagt ccaggcggca ctcatgcagc
     2761 aaccggacaa atctctggca ctgctggcat ggacgctctg cctgaatgtg tttggcagcg
     2821 gagcgtacag taaaccagca caaatcagcc tggaatgtaa acattattcg ctgaccagcg
     2881 atgcgccgtc agggaaggaa ggtgccgcgt tcatggcgct gatggcagaa aaagcccgtc
     2941 ttgcggccct gttaccggag ggatggtcac gggacatgac gacgttcctg tcgctcagcc
     3001 aggaggtgct gttatccctg ctcagtttct gcaccgcgtg cagcatccac ggcgttcaga
     3061 cccgcgagtg tggtcacacg tcacgcagcc cgctggatac gctggagagc gccatcggtt
     3121 ttcacatgcg cgactggtgg cagccgacaa aagcaaactt cttcggacac ctgaaaaagc
     3181 cacaaattat cgcagccctg aatgaggccg gactgtccgg tgccgcacgg gacgcggaga
     3241 agatgaagaa aggtgatgcg gctgaacatg cagagcacca tatgaaagac aaccgctggg
     3301 ttccaggctg gatgtgtgct ccacatccac agacagatgc cactgaacgc accgataacc
     3361 tggctgatgc cgcctgatga acaaccacac cgccccgccg gagacggggc ggcagcaagg
     3421 gagataccgt gatgaaaact gaactgaccc tgaatgccct gcagtccatg aacgcacagg
     3481 aatatgaaga tatccgtgct gcgggaagcg atatgcgccg taatctcact cacgaggtga
     3541 tgcgtgaagt ggacgcaccg gctaactgga tgatgaatgg cgagtatggc agtgagttcg
     3601 ggggcttttt ccccgtccag gttcgtttca cgccagccca cgaacgtttc cacctggcat
     3661 tatgttcgcc gggagacgtc tctcagctct ggatgctggt tctggtgaat tgtggtggac
     3721 agcctttcgc cgtcgttcag gtgcaacata tcttcacgcc tgtcgctatc agtcacacgc
     3781 tggcgcttgc cgcgacactt gatgcgcagg ggtacagtgt taacgacatc atccatatcc
     3841 tgatggcaga aggaggtcag gcatgagcgc acgttcacgg gcactgatcc ccctcagcgc
     3901 agagcaacag gccgccatgc aggctgtggc tgtcacagaa caacgtcgtc gtcagggacg
     3961 cacactttca gcatggcctt atgccagcgc tttctttcgc tgcctgaatg gcagccgccg
     4021 gatttcgctg accgatctcc gcttttttgc tcctgcgctg acgaaggagg aatttcatgg
     4081 caaccgtctc ctgtggctgg ctgccgtgga taaactgatt gaaagttttg gtgaagtctg
     4141 tgttcttccc ctgccatccg atgcggggca tcgtctgttc ccgtccgttc cttttcgtga
     4201 aggtgagcgg cgtcgtcaga aaaccacgct gacagagcag aaatacagcc gccagcggga
     4261 acgtgaggca gaacgacggg aactggaata ccagacatgt tttgctcagg cgcagattga
     4321 ccttgcgttt catactcccg ccacggtcgg aagctggttg tcccgctggt ctggtgttgt
     4381 tgaggagcat gatctggaaa cgattttctg ggggtggtgc gggcgttttc catcactgtc
     4441 atcatttgac cggtttttct ggcaggagga accactctgg cggctgattt ttgaagccgg
     4501 tgaggccggt cgtggtgcac cggtacagat acgtgcactt gagcagtgga tgatcccgaa
     4561 caagctggag aacgcaatat gatgaaatca gacgaacaat atcaggttcc cgtgtggatg
     4621 cgacctctgt tgccgttgct ctgcaatacc ggtggtaatg atccggagga actgctgaat
     4681 gatacagaaa ccaccgccag tgcgaatatt gtccgttatg tactgatagt tgcagtgcgg
     4741 tcgcagattg atctgctgca gctactgtac aggaaaggac tgttgcgcac agagatacca
     4801 ggtggctttt caccggaaga agcgcaggaa ctcctggata atctggtgcg cagccatatc
     4861 agcaaggcgc tgtcgggtga gcgaatggca gcccgtgaca gaaatgccga tctgacctgg
     4921 attcgccagc aactggtcga tgccgcctgg tttgtccgtg ccacactgga agcgcatggg
     4981 atgggcgtcg gaaatgagag tccctctgct ctgccggaga caatgccgga catacagaca
     5041 cgggaactgg ttatgttgat caagcgactg gcatcatcgc tgaaagcggt gaaacccgac
     5101 agtagcgtgg tgcgtgaagc gcaggactgg ctgtgcgaca gaaaacttgt ggatatcaca
     5161 gatattctcc ggtgaaccag gcatgtatat tttacgcagg gacacgccga tcatgatcgt
     5221 ttcatatcag gggtggtctg gaggtcatgc ggcgtgtcct ctgcactcgc cggaataagg
     5281 aagtcgccgg cggctccgct tttacccggc catgcggggc atggccttgt gggttttcag
     5341 ct
//
LOCUS       DAZOXR010000235         5296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZOXR010000235 DAZOXR010000000
VERSION     DAZOXR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.242"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHUNN_003979"
     CDS             complement(<1..130)
                     /locus_tag="ACHUNN_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0578212.1"
                     /translation="MDTRVAFMTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPS"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="ACHUNN_003980"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="ACHUNN_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUNN_003981"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUNN_003981"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR0578213.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUNN_003982"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUNN_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0578214.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUNN_003983"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUNN_003983"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0578215.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="ACHUNN_003984"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="ACHUNN_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAZOXR010000236         5282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZOXR010000236 DAZOXR010000000
VERSION     DAZOXR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.243"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..472)
                     /locus_tag="ACHUNN_003985"
     CDS             complement(<1..472)
                     /locus_tag="ACHUNN_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0578216.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(472..1392)
                     /locus_tag="ACHUNN_003986"
     CDS             complement(472..1392)
                     /locus_tag="ACHUNN_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0578217.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1530..2429
                     /gene="pgrR"
                     /locus_tag="ACHUNN_003987"
     CDS             1530..2429
                     /gene="pgrR"
                     /locus_tag="ACHUNN_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0578218.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2766..4379
                     /gene="mppA"
                     /locus_tag="ACHUNN_003988"
     CDS             2766..4379
                     /gene="mppA"
                     /locus_tag="ACHUNN_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0578219.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTARFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4430..>5282)
                     /gene="ynaI"
                     /locus_tag="ACHUNN_003989"
     CDS             complement(4430..>5282)
                     /gene="ynaI"
                     /locus_tag="ACHUNN_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0578220.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1430 a         1204 c         1225 g         1423 t
ORIGIN      
        1 cgccaatcgg atcgtaattg ctgaaacctt cccgaaaaag acgcccgata ttaacacccg
       61 taatactcac tactgcttta attcgttttt ctgtcagcgc ggcattaatg gcatagccac
      121 cacccccaca cactcccagc acgccaatac gttcagcatt aacatatggc agagttactg
      181 cataatcgat aacacggcta atatcctcta cgcgctgtgt cggatcttct atccagcgag
      241 gctccccacc agattctccc tggaaactgg cgtcataagc gagaacgaga tagccttttt
      301 cagccaatgc cttgccatag atattgccag acgtctgctc cttacaactg ccaaaaggat
      361 ggacgctgat aatcgccgga tactgttttg actcatcaaa atttggtgga aactgaatat
      421 tggcagcaat atgccaggcc atatcactat tgcggatttg aatatgtttc attacagagt
      481 ttcctcaaag aacggagcta atactgaaat tgcttcagcc acataggctt tgccgtcata
      541 aagatccata tggtttgcgc cttcaacaat gtgatagcgt ttatcttggc ttgatgctcg
      601 atcgtacagg tcgtcactca tccatttgct ccctgcctgg ctgcccgcca caatctgcat
      661 cggctgagtc aggtacactt ccgccatatg gtaagcatca aaggtaataa tctggttaag
      721 gctgcgcaaa gtagcgtaac ccggtgctgt tggatactgc gcgcgagggg tgtggtaata
      781 ctcccaggcc tgacgcagtt cttcgttcgg cgcatcggac tccttcattg gtgccagtgg
      841 cataacggca tattctccgc tgctaatatc gctggttcgg gcgtttgaac ccgcttcaac
      901 gtatggcagg gcatcaatag atttcacatt gttttcccaa ccattacgga acatcgaacc
      961 aatattgacc gcactaacgg taccgatggc cttgatgcgg cgatcctgaa ttgcagcatt
     1021 ggctgtatat cctgcaccgg cacaaattcc catcgcacca attcgggtat tgtcgacata
     1081 agaaagcgtt gtcaggtaat caatcacggc actgatgtct tcagtacgaa tgtatgggtt
     1141 ttctaactga cgcggctcgc cgccactttc accttgataa gatgcgtcat aagcaatagt
     1201 gacaaatccc ttttccgcca gttttttggc ataggttccg gccgtttgtt ctttaacgcc
     1261 cccacctggg tgagatacca caattgcctg atactgacgg gtttcatcaa attttggagg
     1321 gaaatagatc actgcagaca aagagatggt tggattattg ctgttagtga agctgacttt
     1381 attattcatc attccgttcc tcatggagtt gtcggttcgt tttaacggtt ggtgatatca
     1441 ctatagatat tgatcattaa gttgattaga cccaaaatta tgattagacc tatcatttaa
     1501 atgattaata gattgcatgg agatactgaa tgaagcgtga agaaatcgct gatctgatgg
     1561 cgtttgtcgt cgttgcagag gagcgtagct tcactcgtgc agcagcccgc ctgagcatgg
     1621 cgcagtcagc tttaagccag atagtgcgtc gtatagaaga acgattggga ttgcggcttc
     1681 tgacgcgaac cacgcgcagc gttgttccaa ctgaagcggg cgagcatctt ttgtctgttc
     1741 ttggccctat gttgcatgac atagattcag ccctggcatc cctgagcgag ctgcagaacc
     1801 gcccatccgg gacaatacgt attactactg tagaacatgc agccaaaacg atattgttac
     1861 cagcaatgcg cacattcctg aaatcgcatc ctgaaattga tattcagctc accattgatt
     1921 atggtttgac cgatgtcgtt tctgaacgtt ttgatgcagg cgtccgtctg ggtggggaga
     1981 tggataaaga tatgatcgcc attcgaatcg ggccagatat accaatggct attgttggct
     2041 caccggatta tttttctcgc cgaagtgttc caacgtcagt gtcacaatta atagatcatc
     2101 aggcaattaa tttgtatctt cccacatcgg gtacagcaaa tcgctggaga ttaatacgcg
     2161 gtggacgtga agttcgtgtt cgcatggaag gtcagctttt actgaatacg atagacctga
     2221 tcattgatgc tgcaattgat gggcatggat tggcgtatct accttatgat caggttgagc
     2281 gggctattaa agaaaaaaaa ctgatacgtg ttttggataa attcacacca gatttacccg
     2341 gttatcacct gtactatcca caccgtcgac atgctggctc ggcattctca ttatttatag
     2401 ataggctgaa gtataaaggt gctgtttagc actacttgct gatacattaa tttaattttt
     2461 ctcttaacgt attctcagtt cctttcaacg ttttggttat tttttattct tcgtacaatg
     2521 gcgacagatg ctgattatga taccgaaaac tggtttgaac gtgcgaagcc tgaacgtagt
     2581 gttcggtgtt ctatgtgctt taccgcattt tgtcgtctat tatttacact aaatctgatt
     2641 taatatattg atacataaaa cattttatgc tccctgtgtc acttttttga tggaagttgt
     2701 ttgcatttct ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga
     2761 aacttatgaa gcactctgtt tcagtcacgt gttgtgcgct gttggtcagc agcatttctc
     2821 tttcgtatgc tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc
     2881 gccacattaa agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga
     2941 ttcaggtcat tcgcgatctg tttgaaggac tggtgaatca gaacgagaaa ggggagattg
     3001 tccccggcgt tgcgactcag tggaaaagta atgacaaccg tatctggact tttaccctgc
     3061 gcgataacgc aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct
     3121 ggcagcgtct ggtggacccg aaaacgctat cgccatttgc atggtttgcc gcgctggcgg
     3181 gaatcaacaa cgcacaggcg attattgatg gtaaagctac gccagaccag cttggcgtca
     3241 ccgcagttga tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga
     3301 atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag
     3361 agtggacgaa acccggaaat ctgatcggca atggcgctta tgttcttaaa gatcgcgtag
     3421 tcaatgaaaa actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtac
     3481 tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacctcg
     3541 cgggggatat tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg
     3601 atattccggg gcaggtttat acgccgccgc agctcgggac ctattattat gcgtttaaca
     3661 cgcaaaaagg gccgacggca gatcagcgcg ttcgtctggc attaagtatg acgatagatc
     3721 gccgcctgat gaccgaaaaa gtattaggga cgggcgaaaa gccagcgtgg cattttacac
     3781 cagatgttac cgccagattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag
     3841 aactgaatgc gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc
     3901 tgaagctgac gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag
     3961 catcgatgtg gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa
     4021 cctatatcga tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtggggg
     4081 attataatga accctccact ttcctgacat tattaacgtc aacgcattca ggaaatattt
     4141 cacgctttaa caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg
     4201 ttaaagcgcg taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga
     4261 ttgcaccaat ttatcaatat accaatggac gattaatcaa gccgtggctg aaaggttatc
     4321 ccattaataa cccggaagat gtggcgtaca gccggactat gtatattgtg aagcattgat
     4381 gtgatgggaa ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgcccct
     4441 gttccggcgg tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc
     4501 cgtgtgactg cacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccatt
     4561 cagcccatac cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcggcaa
     4621 actggttgaa ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca
     4681 tctcacgtac ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa
     4741 tggtcgtggt aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa
     4801 acagcgagtt cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc
     4861 agccaatttc cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa
     4921 tactgaaagg acggtcgaaa tagagcatga tcccggaaaa gaaattactc agaatatctt
     4981 taccggccat accgacagcc agaccaccaa taccaccaaa ggtcagcaag ccagaaaggc
     5041 tcatgccgaa atgttcgcca taaagcagaa caagcaccac aataatggtg attttgataa
     5101 tgcgcgacat aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa
     5161 actgattaat cagcaaaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga
     5221 taaaatcgat aacgtctggg gtgataaatt tgagtttata gttttctata acataattaa
     5281 ta
//
LOCUS       DAZOXR010000237         5277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZOXR010000237 DAZOXR010000000
VERSION     DAZOXR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.244"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHUNN_003990"
     CDS             <1..69
                     /locus_tag="ACHUNN_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578221.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUNN_003991"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUNN_003991"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0578222.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUNN_003992"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUNN_003992"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0578223.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUNN_003993"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUNN_003993"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0578224.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUNN_003994"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUNN_003994"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0578225.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDITTAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUNN_003995"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUNN_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0578226.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1142 a         1394 c         1399 g         1342 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggtttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat tgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatctcc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggtgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaaacg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaatcaa cgaggaaaaa ggggacaaaa
     5221 tgcacgcgtt gcaaaaccta tcctgatgat ttgtattgaa ttatatgttt tgcgatt
//
LOCUS       DAZOXR010000238         5271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZOXR010000238 DAZOXR010000000
VERSION     DAZOXR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.245"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            116..973
                     /gene="focA"
                     /locus_tag="ACHUNN_003996"
     CDS             116..973
                     /gene="focA"
                     /locus_tag="ACHUNN_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0578227.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1028..3310
                     /gene="pflB"
                     /locus_tag="ACHUNN_003997"
     CDS             1028..3310
                     /gene="pflB"
                     /locus_tag="ACHUNN_003997"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0578228.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3502..4242
                     /gene="pflA"
                     /locus_tag="ACHUNN_003998"
     CDS             3502..4242
                     /gene="pflA"
                     /locus_tag="ACHUNN_003998"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0578229.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4374..4511)
                     /locus_tag="ACHUNN_003999"
                     /pseudo
     CDS             complement(4374..4511)
                     /locus_tag="ACHUNN_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            4611..>5271
                     /locus_tag="ACHUNN_004000"
     CDS             4611..>5271
                     /locus_tag="ACHUNN_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0578230.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         1353 a         1302 c         1272 g         1344 t
ORIGIN      
        1 taaaaaccat gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct
       61 catctataat gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa
      121 agctgacaac ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc
      181 gggtgtctat aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg
      241 tgttttcatc tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat
      301 gcccttcggc atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg
      361 tgttgtctgc ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag
      421 tgggcgcatc acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct
      481 ggtcggcgca ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa
      541 tggtcaatgg ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat
      601 tgaggccgtc tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag
      661 ttattctggc cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt
      721 tgttgccagc ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt
      781 aatccgcgac ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt
      841 ttctcacctg accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa
      901 cattatcggt ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa
      961 cgaccaccat taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag
     1021 gtgttacatg tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg
     1081 tgactggcag aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga
     1141 gggtgacgag tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt
     1201 aatggaaggc gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt
     1261 tgcttccacc atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt
     1321 tggtctgcag actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat
     1381 gatcgaaggt tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt
     1441 cactgaatac cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct
     1501 gcgttgccgt aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat
     1561 catcggtgac taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact
     1621 ggcacagttc acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat
     1681 ccgtctgcgc gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc
     1741 tgcgaaatac ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg
     1801 gacttacttc ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg
     1861 tcgtacctcc accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac
     1921 cgaacaagaa gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt
     1981 cctgcgtact ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc
     2041 tatcggtggt atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct
     2101 gaacaccctg tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga
     2161 aaaactgccg ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct
     2221 gcagtatgag aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc
     2281 ttgctgcgta agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa
     2341 cctggcgaaa accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca
     2401 ggttggtccg aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga
     2461 gcgcatggat cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat
     2521 ccactacatg cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga
     2581 cgttatccgc accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc
     2641 tgcaatcaaa tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt
     2701 cgaaatcgaa ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc
     2761 tgttgacctg gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc
     2821 tatcccgact cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg
     2881 taacacccca gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca
     2941 cggtcgtgac cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc
     3001 ttacgctaaa gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga
     3061 cgacgaagtt cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc
     3121 atccatcgaa ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga
     3181 cgcgatggaa aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt
     3241 acgtttcaac tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca
     3301 atctatgtaa ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac
     3361 gaaagtgggg ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg
     3421 actgccaaaa cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc
     3481 cacatctgga gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt
     3541 ggaaccgtag acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc
     3601 tgcctgtatt gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt
     3661 gaagatttaa tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc
     3721 gttaccgcat ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc
     3781 gcctgcaaaa aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac
     3841 gatccggtga ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag
     3901 atgaacgacg agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc
     3961 gccaaatatc tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc
     4021 tggtctgacg atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac
     4081 gttgagaaaa tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg
     4141 ggtgaagagt acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg
     4201 aaaggcattc ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca
     4261 actttattga gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac
     4321 atcttatagt gcatctacca tatcccgtac ctgagataat aacgactgat aatattacgc
     4381 cgatataaat cgagttccgt cacctgtatt ttccgctcca ttgcccgctg atggatcgca
     4441 tcagcaatat gtgcggtcaa tgaatcatga cgaggatggg cccataccaa ataaatacgt
     4501 tcagactgca taattttacc tccacgttaa gttctggagc gcactttacg caggcggcat
     4561 aataatgtaa acgtgatgat tcaggatgag ttaatgaagg attttcacta atgcggatga
     4621 atatgtctga ctttgccact ttctttgctg tggcccgtaa tcaaagcttt cgtgcagcgg
     4681 gcgatgagtt aggcttatcc tcgtccgcca ttagccatag tattaaaaca ctggaacaac
     4741 gtcttaaaat tcgcttattc aatcggacaa cccgaagcgt atcgttaacg gaagcgggtt
     4801 ctaatctcta tgaacgattg cgcccagctt tcgacgaaat tcagatcatg ttagatgaga
     4861 tgaatgattt tcgtctgaca ccaacaggaa cgctcaaaat caatgccgcc cgggtagctg
     4921 cacgtatatt tttaatgcct ttactggttg gtttcacacg tgaatatcct gatattaaag
     4981 tcgagctcac aactgacgac tctcttgtcg atatcgtcca acagggcttt gacgctggcg
     5041 ttcgacttag tggcatcgtc gaaaaagata tgatcagcgt ggcgatcggc ccaccagtga
     5101 aattatgtgt agcagcaacg ccagagtact ttgcaagata tggcaaaccc cgccatcctc
     5161 atgatctgct taatcatcaa tgtgttgtat ttcgttatcc cagcggcaaa cctttccact
     5221 ggcaatttgc aaaggagcag gaaattgctg tcgcagggaa tatcattctg g
//
LOCUS       DAZOXR010000239         5244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZOXR010000239 DAZOXR010000000
VERSION     DAZOXR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.246"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHUNN_004001"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHUNN_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUNN_004002"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUNN_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR0578231.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHUNN_004003"
     CDS             complement(1327..3060)
                     /locus_tag="ACHUNN_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0578232.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHUNN_004004"
     CDS             complement(3057..3551)
                     /locus_tag="ACHUNN_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0578233.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHUNN_004005"
     CDS             complement(3673..4023)
                     /locus_tag="ACHUNN_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0578234.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHUNN_004006"
     CDS             complement(4066..4530)
                     /locus_tag="ACHUNN_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0578235.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUNN_004007"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUNN_004007"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR0578236.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="ACHUNN_004008"
     CDS             complement(5024..5239)
                     /locus_tag="ACHUNN_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0578237.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1148 a         1534 c         1299 g         1263 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tccc
//
LOCUS       DAZOXR010000240         5230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZOXR010000240 DAZOXR010000000
VERSION     DAZOXR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.247"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..319
                     /gene="gatA"
                     /locus_tag="ACHUNN_004009"
     CDS             <1..319
                     /gene="gatA"
                     /locus_tag="ACHUNN_004009"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0578238.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
     gene            350..634
                     /gene="gatB"
                     /locus_tag="ACHUNN_004010"
     CDS             350..634
                     /gene="gatB"
                     /locus_tag="ACHUNN_004010"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0578239.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            638..1993
                     /locus_tag="ACHUNN_004011"
     CDS             638..1993
                     /locus_tag="ACHUNN_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0578240.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            2041..3081
                     /gene="gatD"
                     /locus_tag="ACHUNN_004012"
     CDS             2041..3081
                     /gene="gatD"
                     /locus_tag="ACHUNN_004012"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0578241.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            3187..3960
                     /locus_tag="ACHUNN_004013"
     CDS             3187..3960
                     /locus_tag="ACHUNN_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0578242.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4042..4941)
                     /gene="yegS"
                     /locus_tag="ACHUNN_004014"
     CDS             complement(4042..4941)
                     /gene="yegS"
                     /locus_tag="ACHUNN_004014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0578243.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
BASE COUNT         1311 a         1153 c         1341 g         1425 t
ORIGIN      
        1 agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc
       61 gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca
      121 gcaagcggat gatgataacg acgtggaggt atcgttggtt attgcgttga ttgtggagaa
      181 tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat
      241 cgtcgagaca ctaatcactc ttcctgaaac ccagttaaag gaatacttca caaagtatgt
      301 tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa
      361 gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg aagaaattaa
      421 agagttgtgt cagaatcata gtattcctgt tgaattaatc cagtgtcggg ttaatgaaat
      481 agaaacctat atggatggtg tgcatttgat atgcaccact gccaaagtgg atcgtagttt
      541 tggcgatatt ccgttagttc acggcatgcc ttttatttct ggtgtcggta tcgaagcatt
      601 acaaaataaa attctgacta tcttacaggg gtgacctatg ttttcagaag tcatgcgtta
      661 tattctcgac ctcggcccta cggtgatgct tccgattgtc atcattattt tttctaaaat
      721 attaggcatg aaggcaggcg attgctttaa agcgggtctg catatcggga ttggctttgt
      781 tggcattggc cttgtgattg gcttaatgct ggattccatt ggtccggcgg cgaaagcgat
      841 ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg gctcttcacc
      901 aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg attgcgattc tggttaacgt
      961 ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga atatctggca
     1021 tatgaccttc accggcgcgt tgctgcatct ggcaaccggt tcatggatga tagggatggc
     1081 aggtgtggta attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac
     1141 ccgaaatttc tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg
     1201 gccgattgcg gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa
     1261 atttagcgcc gacgatattc agcgcaaatt tggtccattt ggcgagcctg tcaccgtggg
     1321 ttttgtgatg gggctgatta tcggcatcct cgccggttac gatgtcaaag gtgtattgca
     1381 gctggcggta aaaacggcgg cagttatgct gctgatgcca cgggtgatta aacccatcat
     1441 ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg
     1501 tcaggagttc ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc
     1561 ggcaagcctg atttttatcc cgctcaccat tttaattgct gtttgtgttc cgggtaatca
     1621 ggtactgccg tttggcgatc ttgccaccat cggcttcttc gtggcgatgg cggtcgcggt
     1681 gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc atcattatga gcatcaccct
     1741 gtggatcgcg acgcaaacta ttggtttgca cacccaactg gcggctaatg ctggggcgtt
     1801 aaaagccggg ggtatggtgg cttcaatgga tcagggcggt tctcccatta cctggttact
     1861 gattcaggtt ttctccacgc aaaatattcc cggtttcatt attatcggcg caatttatct
     1921 taccggtatt ttcatgacct ggcgtagagc gcgtggtttt attaaacaag agaaaaccgt
     1981 tctcgcagaa taatttttac ctgaggggga ggttatcccc cttaacaatc aggagttttt
     2041 atgaaatcaa tggtgaatga tactgatggt atcgtgcgcg ttgcagaaag cgtcattcct
     2101 gaaattaaac atcaggatga ggtgcgggta aaaattgcca gctcgggctt atgtggttcc
     2161 gatttaccca ggatatttaa aaatggtgca cattattatc caataacgtt aggccatgaa
     2221 tttagcggct atattgatgc ggtgggatcc ggtgttgatg atttacaccc tggcgatgcg
     2281 gttgcctgtg tgccgttatt accctgtttt acttatccag agtgtttgaa agggttttat
     2341 tcccagtgcg caaaatatga ttttattggc tcgcggcgtg atggtggatt tgctgaatat
     2401 attgtcgtta agcgaaaaaa tgtctttgct ctacccacgg atatgcctat tgaggatggg
     2461 gcttttattg agccgattac cgttggcctg catgcttttc atttagcgca aggttgtgag
     2521 aataaaaacg ttattattat tggtgccgga accattggcc tgctggctat tcagtgcgct
     2581 gtcgcgctgg gagcaaagag tgtgacggcg atcgacatta gctcagaaaa actggcattg
     2641 gcaaaatctt tcggtgcgat gcaaacattt aacagtagcg aaatgagcgc gccgcaaatg
     2701 cagagcgttt tacgcgaact gcgctttaat cagcttatcc tcgagacggc tggcgtaccg
     2761 caaactgtcg aactggcggt agagattgcc ggtcctcatg cccaactggc gctggtgggc
     2821 acgttgcatc aggatctgca tttaacatcg gcaacgtttg gcaaaatatt gcgtaaagag
     2881 ctgacggtta tcggcagttg gatgaactat tccagccctt ggccggggca ggagtgggaa
     2941 acggcgagcc ggttgctgac agaacgtaag ttaagcctgg agccattaat cgctcaccgt
     3001 ggaagctttg aaagcttcac ccaggcggtg cgtgacatcg ctcgtaatgc catgccgggc
     3061 aaagtgttgc tcattccatg aaaccgcggg ccagcgtgat gctggcccgg tattgtgcaa
     3121 aacagattat tcaccaatgg tcccccttcg tttacactag ccacaattga atatgggtaa
     3181 atgacgatga attcattcga gcgaaggaat aagatcatcc aattagtgaa tgaacaggga
     3241 accgtgcttg ttcaggatct ggcgggagta tttgctgcct cggaagcgac aatccgtgcc
     3301 gatttgcgct ttctcgaaca aaaaggcgtg gttacgcgct ttcatggcgg tgcggcgaaa
     3361 ataatgtccg gtaatagtga aaccgagacc caggaagtcg ggtttaaaga gcgatttcag
     3421 ctcgccagcg cgccaaaaaa cagaatagcg caggcggcag tcaaaatgat ccatgaaggg
     3481 atgaccgtta ttctcgacag cggaagtacg acaatgctta tcgccgaagg attaatgacc
     3541 gctaaaaata tcacggtgat taccaacagt ctcccggcag cgtttgccct ttccgaaaat
     3601 aaagacatta ctctggtcgt ctgtggtggc acggtccgcc ataaaacgcg ctcgatgcat
     3661 ggttccattg ccgagcgttc attgcaggat attaatgccg atttaatgtt tgtcggtgct
     3721 gatggtattg atgcggttaa tggtattacg acctttaatg aaggttattc gattagcggt
     3781 gcgatggtca ccgccgctaa taaagtgatt gccgttctcg attcatcgaa atttaaccgt
     3841 cgcggtttta atcaggtact gccaatcgaa aaaattgaca ttattattac cgatgatgct
     3901 gtatcagagg tggataaact ggcattgcag aagacacggg taaaattaat tacggtgtag
     3961 tattgttctg tatgccggat aaggtattta caccgcatcc ggcatttcgg attctccctg
     4021 gttcaagaga gaatagtttg attaacgcaa taatagacaa tctggtggta atcgacaacg
     4081 caacgccgcc ggaagtattt caatatgaaa gttttgtccg ctcaacggtt cgccatcaag
     4141 attaaaagtg atttcgtgtg gggcttgtat atcaaaccac gacgaagcgc cttcgataat
     4201 attcgggtta tcttcgtcag attttaaggt tgatacgaga gccggaagaa tttcatcgcc
     4261 ggtaaaaatg cgcagttgca gcaagccatc gttaattaac gcgttcggac acaattgctg
     4321 accgccaccg gcctgacgcc cgttaccaat accaatgacc agggcgtcac cttgccagtg
     4381 aaagttttca ccgcggattt cacaacggtc cggttgcaga gtatccatgc gcattaagcc
     4441 atgaatgatg taagagacgc cacccagcgc ggcttttaat ttttccggcg tttctgtggt
     4501 aatacgcgtc ccaaatccgc ctgtcgccat attaataaaa caggtttgtt tgttgacctg
     4561 cgccatatct atcgcaatgg cgttaccggc aattgccagt ttcagcgcct tatccagtgc
     4621 ctcaggaatc cctacactgg tggcaaaatc attggcggtt cctaatggca aaattcccag
     4681 cgcgggtatg ccatccccct cacactgaat caacgccgta gaaacttcat taatggtgcc
     4741 atcgccacca ccggcaatca ccgttgcgac gcccaacttc cgggcctcct ctacatatcg
     4801 tgcggcatcg cctttctccc aggtgacccg cacatggatc gtcattcctt cctcacgcaa
     4861 cagcataatt gcttcgcgca agggtagatt gtcagtactt ttgccattaa gaatcagtaa
     4921 gctggcggga aattctgcca tgatcgtatg tgcctttatg attggtctgt agatagtgta
     4981 gagcagaaaa caaaaggtgg agtcagaaca ggatgaaagt cggaggataa aaagtcagtt
     5041 caagtatgga ggattcagtg gttaccacca ggagcttgca atggggacag cgaatactaa
     5101 cggctgccac cgataaattt caaaaaagag catataccta atattcaact aaacagtggc
     5161 atcttcaata taatatatta aagcccccat ggagttaccc tgaagggcct caatgtccgt
     5221 aattcctact
//
LOCUS       DAZOXR010000241         5228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZOXR010000241 DAZOXR010000000
VERSION     DAZOXR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.248"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..2779)
                     /locus_tag="ACHUNN_004015"
     CDS             complement(<1..2779)
                     /locus_tag="ACHUNN_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0578244.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLP"
     gene            complement(2836..5208)
                     /locus_tag="ACHUNN_004016"
     CDS             complement(2836..5208)
                     /locus_tag="ACHUNN_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0578245.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1298 a         1220 c         1195 g         1515 t
ORIGIN      
        1 ttggcaataa tactccagta taagtattta ccggatgaga aagatattgt ttaacggcag
       61 tgttaacatt ctctaccgtc atttgtttca acaattgctc ctgctcagtc catgctgcag
      121 gatcgtcata ttgaataaga ctatttacaa tagtgttcgc taattgttga acgctacgct
      181 gttggatatc gaggctgcgc tgaacgtttt gctggtattc attcagttct tgctcactga
      241 tccctttagc cagacgctta accatcactt cattcgctaa cgttaacagt tcatcatgtc
      301 gttctggttg acaagtaaaa gccagcaaat gactgatatc tttggcctga ggatcaaccg
      361 agaggcgaga agaaacgctg tatgctccag atgcctgttc acgaatatta acgcgtagat
      421 cttttgccag tgcgacgtta aaagcatcga gcgccatacg cgtcgccaga ttaacaggtg
      481 tccgggaatc ataacgcttc cactgtgaaa cctgtgccac aggttcattt tgttctttta
      541 cagtaaccga tgcgttgtcc gtcgcgcgag ttaatggttt acctgcggct aatggcgaat
      601 cagagtgttt gattgatcct aagtaacgcg taattaacgc cacgagtttg tcttctgaga
      661 cattaccgac aatgacaaac gtgatatccg ctggagatga aaacaactgg cgatcggcag
      721 ccagcgcatc tgcggcagta aactgtacaa tctgattttc ttgcagtaat ttcgtgcggt
      781 catcagcata gcgcgtctca tacatctgct gggcgaattt ctccgctgga cgctggtcga
      841 gcgttttcaa cgcctgaatt tgagcatttt gtagcgatgc ccaaatattg tcgttaatcg
      901 tgctgtgggt gattcgctgg ttaatcaact gaaaaccagg ttcagggtta ttagtccgcg
      961 cgctaacgga gagcaacgta ttcatgccac tgactttact gctcatggtt accgaatttt
     1021 ccgcactcca gcgtttcagg ctggaagagg agagctcacc aacgccgctt ccgcttactg
     1081 ctttatttgc cagcgcgata agtgattttt gctgcgcagg gaaacttaaa tcgcctttat
     1141 tagatacggc agtaatttgt agcttttgct cttcacccgc ggattttgcc agaataaccc
     1201 tggcaccatt ggaaagtgtt aatgatgtca gattctcagc cagcgtttct ttgctgctaa
     1261 tttccgcctg tgggtcagcg tctactgtta acgataaatt tctgcctggg aagatgtaag
     1321 ccgccagctt tttgttggcg tactcttttt ccagcgccag gatagccgca agagacaatg
     1381 cttttttggc ggcaagctca ttgtttacca tttgctccca aaatgcgtcc tggttctttc
     1441 ttaactgctg ccatttttcc gccagacttt gcacggtaat ttgctgccac agacgtttcg
     1501 aaagttgata tgtctcttcc ggcgacaaga acggcgtatt atttaatgag ctggatgcca
     1561 ggcgactggt cagcatacgt aaatcacgtt cagcttgctg atcaaccgca tttttcagcc
     1621 aggtgaggcg ggtagatttg acatcatcga gttcttcagc agaaaaacca tgctgatcaa
     1681 tggttgccaa ctctgccatt aatgcatttg cagcatcctg catattatcg tctcgtgcat
     1741 ttacacggaa aaacagcgac tgataatcgg gtgcaatttt aacgctgcgc gcagtgccgc
     1801 cagaaatagt cttcaactcg cccgactgta tgcgttcctg cagacgttga ttgaacagct
     1861 gaactaacat gctccattca gcttgttcga caaagctttg ctcatcgttc acttgtacca
     1921 ttggcaggcg ataatagagt gcgatgccgt tcactcggtt ttctttatca ttaatgatat
     1981 taaagcgcag gtggttttcg gcttttgtcg gccagacgcg attttcagct gctttgttag
     2041 ccggaagctt acttaaatta tcctttatca gcgccagcgc ttctttactg tcgatatcgc
     2101 cgaccacgat aaaggtcata ttatttggtt gataccagcg ttgataaaat tggcgcaatt
     2161 gtgccggtgt gaccgtggcg acagtatcca tcaggccgat aggttcacgg tctaaattac
     2221 gggtatttgc cagcaggaaa gggcggcgcg cctgagaggt gcgccatttc gcatcctgat
     2281 gggcacgcca ttcctcagta attacgccgc gttcagcgtc tacttcgagt atttcaaagg
     2341 ttgcggcatt actccattca ctgaagattg ccatcacttg ttgcagattt tgtttctgcg
     2401 tagtcggcag actcacctga tacagcgttt cgtcatagct ggtataggca ttaacatcgc
     2461 gaccgaaacg caggcccatt gactcaaatg tttcgatgac tttattaccc ggccatgttt
     2521 ttgtgccgtt aaacatcata tgttctacaa aatgagccac gccgcgctca ttgtcttctt
     2581 cctgcaatga accggtatga atttgcagcc ataaatttac ctgatccttt ggttgagcat
     2641 gcggataaat catatatcgc aagccattgt ccagttgccc ggtaattaac ttttcatcct
     2701 gcggtaaggc ggcggcaatc agccgcccgg ggagcaacag agttgccact aacgtcagta
     2761 agaaacagag gtttctcata attatctcca tgcgaaaacc gggcgaattt acccggttaa
     2821 gtaaaatccg aactattaaa atttcatgct gacatcaagc cagaaagtac ggccactggc
     2881 gtaggtcgcc acaccggtgt tcgttgtatc aacagcggtt ttgctatcca gtacattgag
     2941 aatatcggca ctgatcgtca ggttttgttg tttcagaaat tgtggcgtcc aggacaagcg
     3001 ggtgtcccag gtcaggctgc tatccagttt ttcgtcaaca tactgcttgt aatctgaata
     3061 ttcgctgatg tattgcgcat ttgtcttacc aaggataatg cgagctttac gcgcttcttg
     3121 ccaggccagc gtattcgccc acactaaccc gctcggttga tgcgtgaaat ccatgtttaa
     3181 ggagatcttt aatgggttat taaaatctgc cactggaaca ctatcgtaag agaccagatt
     3241 accgttataa accacctggt tatcaccggt attgctctcc tcataaccat tgtttaacga
     3301 caagttgccc ttgctcttga tatagctaaa gacaatttgt gggttaatat ctacttggcg
     3361 gatatgcagg ggttcggcca gttcaaaact gaggctgaac gaatgcgttt tggttttgcc
     3421 atcgttgtta tattcagtaa tggtggtttt agtcgcgctg tcggtacgac tgcttttgct
     3481 gatttgatca tgcgcttcac ggtaaacata gtttgcacgc gcaataacgt tcttaccgat
     3541 tttctgctgc aatcccattg ccagttcatc gttataaggc gttttcaaat cctgataacg
     3601 cgtcagagtt ttattacctg ataccgattc cgtccagcta ttgcggatat cacgtaatcc
     3661 catatcaaga atattcccgc catagtaacg gttataaccg gcggtaatca ttgaggtttg
     3721 atcagcaaaa atatcccatt ccgtcataaa gcgcggggag atattgtggt ttgacagata
     3781 gttgtcatag tcataacgca caccgggcat taacgacaca ttccgccagc taatgtgctc
     3841 cgccatatac agcgtgtagt tgtcaattcc caggctgcct ttacctttat ggtaaatggt
     3901 atggttagtt ttctttccgg cagcattaat cacataggat tcagactggt tatggcgttc
     3961 agtccatgca tcggaataga tgtattccgc gccgaagtag ggttgatgcg aaacatcacc
     4021 cacggcgaat ttttgccagt ccaggcgtgt tttgaaggtg taattatcta cagcctggga
     4081 aatgtgtcct aatccgccac aggtacaacg ccctgtaata tcaccatatg tacatgaaag
     4141 ttcggtgtac cagatgtcat gatcgtgacg ggtataatca ctaatatgat cccaaccaac
     4201 ggtggtacgt agtttggccc atgcgagttg cgtatccata tcccatgcca gaccatagga
     4261 tttattaccc atttcgcgat cagactgcgg gaaggtgctg gtattataat cacggctgga
     4321 gccggtatat tttaaggtta aatcgtgggt aaagcggtcg ctggcaaacc aggtaaattt
     4381 gctcaatgca gtatcgataa cgtttttata ctgtgcccga ccggtgacaa tgccgtcatt
     4441 cgaaacataa tccgcgcggg tgatatcaga ctggcggcgc gataaaccgg cggtaacgcc
     4501 gaagttatca gcgagttcct gattaaacga caaggtataa aagttctttt taaaatctgg
     4561 ggagtaataa gtacttcctg aagagccttg attaaatgcg ctcttgttat tctcatcgat
     4621 atgcgatgtt aaccagtccg aacgcgtagt gcgataaccc aatttcacct tgctatcatc
     4681 agcgttgaag cgtttgatct ttgcatcaat taccccgcca ttgaagcgac caaattcaac
     4741 cggcacaaaa ctgtcataaa gcgtcacatt gtccagtaag ctgacatcaa gataataccc
     4801 ctgtgacatc ccgctaatat tggttgcact actggcatcg gactcattcg ctgggttcag
     4861 gttattagtt gcactaatac cgtcaatcaa ataggcattc tggtagggcg acgcaccgtg
     4921 aatagagatt ttctcagggc gaatatctcc ctggttcaac gaggtacttt gcgttgaatc
     4981 catgcgtacc gcagggttgg ttctcagcag atcgctgata ttgccattcc cggtcggtaa
     5041 acgctcgatg ctttcactgg tgtaatgcgt gttgccgttt acgggtacag ggacaggagc
     5101 atagaccgtc atctcttcaa aaaaatgcat gtacatgttt ttgtcatcga cggcctgcgc
     5161 cacatcagcg ccacataaaa tgacgccagg aataagaact cgcttcattt atttatatct
     5221 ccgtacag
//
LOCUS       DAZOXR010000242         5219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZOXR010000242 DAZOXR010000000
VERSION     DAZOXR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.249"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..227
                     /locus_tag="ACHUNN_004017"
     CDS             <1..227
                     /locus_tag="ACHUNN_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578246.1"
                     /translation="KNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPA
                     IIHSALNPTHYYQPVNDETTVMEIIKAYSK"
     gene            complement(291..575)
                     /locus_tag="ACHUNN_004018"
     CDS             complement(291..575)
                     /locus_tag="ACHUNN_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="HFR0578247.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="ACHUNN_004019"
     CDS             complement(593..790)
                     /locus_tag="ACHUNN_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578248.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="ACHUNN_004020"
     CDS             1002..1298
                     /locus_tag="ACHUNN_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578249.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="ACHUNN_004021"
     CDS             complement(1843..2076)
                     /locus_tag="ACHUNN_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0578250.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="ACHUNN_004022"
     CDS             complement(2083..2613)
                     /locus_tag="ACHUNN_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0578251.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2651..3349)
                     /locus_tag="ACHUNN_004023"
                     /pseudo
     CDS             complement(2651..3349)
                     /locus_tag="ACHUNN_004023"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="ACHUNN_004024"
     CDS             complement(3291..3614)
                     /locus_tag="ACHUNN_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0578252.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="ACHUNN_004025"
     CDS             3679..3870
                     /locus_tag="ACHUNN_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0578253.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4538..4651
                     /locus_tag="ACHUNN_004026"
     CDS             4538..4651
                     /locus_tag="ACHUNN_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0578254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
BASE COUNT         1373 a         1427 c         1116 g         1303 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagagagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca ggcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAZOXR010000243         5147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZOXR010000243 DAZOXR010000000
VERSION     DAZOXR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.250"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHUNN_004027"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHUNN_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0578255.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUNN_004028"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUNN_004028"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0578256.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHUNN_004029"
     CDS             2475..4124
                     /locus_tag="ACHUNN_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0578257.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5147)
                     /locus_tag="ACHUNN_004030"
                     /pseudo
     CDS             complement(4278..5147)
                     /locus_tag="ACHUNN_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1113 a         1201 c         1388 g         1445 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtc
//
LOCUS       DAZOXR010000244         5114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZOXR010000244 DAZOXR010000000
VERSION     DAZOXR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.251"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..379
                     /locus_tag="ACHUNN_004031"
     CDS             <1..379
                     /locus_tag="ACHUNN_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578258.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="ACHUNN_004032"
     CDS             complement(575..2125)
                     /locus_tag="ACHUNN_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0578259.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="ACHUNN_004033"
     CDS             2297..2743
                     /locus_tag="ACHUNN_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0578260.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="ACHUNN_004034"
     CDS             complement(2937..3872)
                     /locus_tag="ACHUNN_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0578261.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4393..>5114
                     /locus_tag="ACHUNN_004035"
     CDS             4393..>5114
                     /locus_tag="ACHUNN_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578262.1"
                     /translation="MNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMTVGK
                     AFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSIAQN
                     NNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYLDRM
                     LYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKNASP
                     LITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAZOXR010000245         5044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZOXR010000245 DAZOXR010000000
VERSION     DAZOXR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.252"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUNN_004036"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUNN_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1771
                     /locus_tag="ACHUNN_004037"
                     /pseudo
     CDS             1595..1771
                     /locus_tag="ACHUNN_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1779..1868)
                     /locus_tag="ACHUNN_004038"
                     /pseudo
     CDS             complement(1779..1868)
                     /locus_tag="ACHUNN_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUNN_004039"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUNN_004039"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0578263.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="ACHUNN_004040"
     CDS             complement(3750..4700)
                     /locus_tag="ACHUNN_004040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0578264.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="ACHUNN_004041"
     CDS             complement(4839..>5044)
                     /locus_tag="ACHUNN_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0578265.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAZOXR010000246         4882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZOXR010000246 DAZOXR010000000
VERSION     DAZOXR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.253"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(358..1401)
                     /locus_tag="ACHUNN_004042"
     CDS             complement(358..1401)
                     /locus_tag="ACHUNN_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0578266.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1813..3025)
                     /locus_tag="ACHUNN_004043"
                     /pseudo
     CDS             complement(1813..3025)
                     /locus_tag="ACHUNN_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3297..3391)
                     /locus_tag="ACHUNN_004044"
     tRNA            complement(3297..3391)
                     /locus_tag="ACHUNN_004044"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Sec,seq:tca)
     gene            3684..>4882
                     /locus_tag="ACHUNN_004045"
     CDS             3684..>4882
                     /locus_tag="ACHUNN_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR0578267.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1276 a         1069 c         1117 g         1420 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccggaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaaataata taataacaaa
     1741 aatcctccag ccttgtaggc cagaggataa aatacttaat tgaggaaaat atcagggaaa
     1801 agtatttctg acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg
     1861 catcatttca cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt
     1921 tttatccaca tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
     1981 aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa
     2041 ttcaattact tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat
     2101 aaagactagg ggatgatgtt ttgagattgg ttcaagctgc ttaaatagca taatgacctg
     2161 ctcagagagc ggcacaatat gaggtcgctt catcttcatt acttctgccg ggatctccca
     2221 caattttgtc tcaaaatcga tatcttccca gcgtgcaaaa cgcagttctt gtgttcgtac
     2281 gccggttaac ataatgatct gagtagctgt cttagtgatt atacttccgg tataacccgc
     2341 cagatcgttg aggaagtggg gaagttcatt agcagtaaga aagggaaaat gtactttttt
     2401 cggtgtagct aaagcactgg caagatcggg ggctgggtta tagtctgccc ggccagtaac
     2461 aattgcatag cggaacacct caccacagcg ctggcggacc ttgcgcattt tctccagagc
     2521 tccacgtttt tccattttac gcagtgcttc cagtaactcc attggtttga tttcggcaat
     2581 agggcgcttg ccaatgtagg gaaaaatgtc tttttcaaag gtatcaatga tttcatcacg
     2641 atagcgtaat gaccaccgat cagcttttga ctggtgccat tcgcgagcaa tagcctcgaa
     2701 cgtatttttc agactcattt gctgcgcgat tttttcttcc ttttttgcct cccctggatc
     2761 gcctccagca gcgaggagtt tcctggcttc actgcgtttc tcgcgagcat cggctagcgt
     2821 tacatcgggg tatacgccaa tagaaagcat cttttcttta ccagcaaagc ggtacttcat
     2881 gcgccagtat ttagaaccat tagtgttaat cagtagatac ataccgccgc catcagcaag
     2941 cttatagggt ttgtctttgg gttttgcggt tttcactttg atatcggtta atgccatgac
     3001 atcctccggc ttttgatgaa ttcatgttcc tgagggggat ttctgccagt aactaacctg
     3061 atacccccaa tacacccctt gtataacaaa gatattgtta aaagccgttg ttatgtatca
     3121 catttttcct attatggggg tgcttgtatt tcaataggaa ataacacccc ctaaagtacc
     3181 cccaaaagaa acttgatatg gggtgaagta ggttgacctc aggatacaaa taaatactga
     3241 tatacaaggg attagaagag atttattgac attgggagag ttcagaagag gtaaactggc
     3301 ggaagatcac aggagtcgaa cctgcccggg accgctggcg gccccaactg gatttgaagt
     3361 ccagccgcct caccggagac gacgatcttc cgcgcctcga ttgctacatg gaggcggggc
     3421 gcattatagc tacttccttg agtttctaca tcccccagat cagttttttc cgcctaatac
     3481 acgcagcgta ttatcacgaa agcattataa ctcatataaa acccatattt tacaatgata
     3541 tatgagcgga attaacgcta ccacgatcac ataaaacaag aatcataaat taataaccag
     3601 atatcggaat attcgctctc acagggatgg tttacaaaat gcgttatcgg tctcagcacc
     3661 cctatagcat caaggaaaag cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg
     3721 gttatggcat gggagacgcc gccagccaca ttattttcga taacgtaatg ttatatatga
     3781 tgttctttta taccgatatt tttggcattc ctgccggatt tgtcggaacc atgtttttgg
     3841 tcgctcgtgc actggatgcg atttccgatc cttgcatggg gttgttggcc gatcgaacgc
     3901 gctctcgctg gggtaaattt cgtccgtggg tactgtttgg cgcactgcca ttcgggatcg
     3961 tctgtgtact ggcctatagc acgccagatc tcagtatgaa cggcaaaatg atctatgcag
     4021 caattactta caccctactt accttacttt ataccgtcgt caatatccct tactgcgcat
     4081 tgggtggtgt aatcaccaat gacccgactc agcgtatctc gctgcaatcc tggcgttttg
     4141 tgctggcgac cgcgggaggc atgctttcta ctgttctgat gatgccactg gttaatttaa
     4201 ttggcggtga taataaacca ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg
     4261 cattcatgat gctggcattt tgtttcttca ccactaaaga acgcgttgaa gcaccaccta
     4321 caacaacgtc tatgcgggaa gatttacgtg atatctggca aaacgaccag tggcggattg
     4381 tcggtttact aaccattttc aatatcctgg cggtgtgcgt acgcggtggg gcgatgatgt
     4441 attacgtcac atggattttg ggcacgccgg aagtgtttgt cgcttttctc accacttatt
     4501 gcgtgggtaa cctgattggt tccgcactgg caaaacctct gaccgactgg aaatgtaaag
     4561 tcactatctt ctggtggacg aacgccctgc tggcagtgat tagcctcgcg atgttctttg
     4621 ttcccatgca ggccagcatc actatgtttg tcttcatctt cgtgattggt gtgttgcatc
     4681 aactggtgac acctatccag tgggtaatga tgtccgatac cgtcgactac ggcgagtggt
     4741 gcaatggtaa acgcctgacc gggatcagtt ttgctggcac gctgtttgtg ctcaaactgg
     4801 ggttggcctt cggcggcgct cttatcggct ggatgctggc ttatggcgga tatgatgcgg
     4861 cagaaaaagc gcagaacagc gc
//
LOCUS       DAZOXR010000247         4860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZOXR010000247 DAZOXR010000000
VERSION     DAZOXR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.254"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUNN_004046"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUNN_004046"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0578268.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUNN_004047"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUNN_004047"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0578269.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1091 a         1355 c         1248 g         1166 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttaatta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atattttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa tagaagacaa
//
LOCUS       DAZOXR010000248         4806 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZOXR010000248 DAZOXR010000000
VERSION     DAZOXR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.255"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(47..2116)
                     /gene="glyS"
                     /locus_tag="ACHUNN_004048"
     CDS             complement(47..2116)
                     /gene="glyS"
                     /locus_tag="ACHUNN_004048"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0578270.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2126..3037)
                     /gene="glyQ"
                     /locus_tag="ACHUNN_004049"
     CDS             complement(2126..3037)
                     /gene="glyQ"
                     /locus_tag="ACHUNN_004049"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0578271.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3132..3431)
                     /locus_tag="ACHUNN_004050"
     CDS             complement(3132..3431)
                     /locus_tag="ACHUNN_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0578272.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3606..4601
                     /gene="wecH"
                     /locus_tag="ACHUNN_004051"
     CDS             3606..4601
                     /gene="wecH"
                     /locus_tag="ACHUNN_004051"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0578273.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4643..>4806)
                     /locus_tag="ACHUNN_004052"
     CDS             complement(4643..>4806)
                     /locus_tag="ACHUNN_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0578274.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1097 a         1265 c         1221 g         1223 t
ORIGIN      
        1 aataaaaaaa gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg
       61 aaatatccgc aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga
      121 gacgcaattc tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct
      181 cacgcagctc tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct
      241 ccagcttgtc acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca
      301 gggtagaggc attcacgcgg tcgctcagca cttcgtcaga tttcgccaga atgttagata
      361 cacgtttgtt cgccgccgcc agtgcagcag ctgcatccag ggtacggaaa tgcgataccg
      421 ctttcatacg ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga
      481 tggtgtcaac ggtgtaacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt
      541 cgataacatc atcaactacg ttggcgttag tcagcttatc gccatacaga cgcactgctt
      601 cttcggtcag cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc
      661 caagtgcggc acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac
      721 cgaagatacc cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt
      781 tggacggcag gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta
      841 cgtcttccgc ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg
      901 tgaactcgaa gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg
      961 cgtggttaac gtcagcgcca atctgttcag caatccagcc agccagcgcc tggatgcggt
     1021 cagttttgtc acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca
     1081 ggttatcttc cagacgtttt ttacggtcgg tattgaagaa gaactcggca tccgccagac
     1141 gtggacgaac gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt
     1201 tggcaacgaa gatgaagttc ggcagcagtt tgccatcgtt cgcatacacc gggaagtatt
     1261 tctggtcacc tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt
     1321 cgaattttgc ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc
     1381 tttcgcttaa gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa
     1441 tcttcgcttt acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg
     1501 gatactgatc ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa
     1561 tcacgcgatc ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca
     1621 gggtcacggt gtgcaccgga cgcacgaagt gcacgtcgct tgcgccccaa cgcatcagtt
     1681 tcgggatcgg cagtttcgcc agcgaagtcg caaccatatt cggcagtagt gcttcagtgc
     1741 tttcgccctt cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct
     1801 cagcctggtc aacggtaata ccgcaaccac gcgcccaacc ttctgccgct ttgctcggtt
     1861 tgccttcagc gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg
     1921 gttgcgcttc cgccaggtta gctactttca acgccagacg acgcggagca gcaaaccatt
     1981 gaacggtgcc gtgtgcgagg ccagcgttat ccagctccgc agtaaagttc gcagcaaagg
     2041 actcagccag gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa
     2101 aagttttctc agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg
     2161 cttcacggga agcgtagtat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa
     2221 tgtagcgctg acgctcagtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt
     2281 gggcggcttt cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca
     2341 gctgctgcgc ttctttctcg tactgctcga agcaggtgaa caggaaatcc acatccgcgt
     2401 attcgaagtt gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg
     2461 ttttacccag cgggccgtcg ctccagacca ggtcgtaaac gctgtctacg ccctgaatgt
     2521 acatggccag acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac
     2581 caccaacctg ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc
     2641 agcccagtcc ccaggcaccc agagtcgggt tttcccagtt atcttccacg aaacggatgt
     2701 cgtgaatagt cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat
     2761 tgtccggcga tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt
     2821 tttcgccgta gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg
     2881 gttctggccc cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca
     2941 tgtccaatgg ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga
     3001 tcaagccctg gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt
     3061 ggatttaaag gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga
     3121 aaaagcaggg cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag
     3181 acaaactgct ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg
     3241 aaggcggtga cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg
     3301 cataacgctt ccatattcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc
     3361 acgggtgacg gtgtagacca acccactagc accataagcg ccattgccgg aaagaaagcg
     3421 ttcatcatca ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata
     3481 gtggctgata tcttacgaat ttccttaagc ctctataagc tgtagtgata aaactcagat
     3541 tattccagtg cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga
     3601 cacagatgca gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg
     3661 tggtgatgat tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca
     3721 catgggatat cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca
     3781 tgatttccgg ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta
     3841 tcggcttatg tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct
     3901 ccatcaatat ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt
     3961 ggtttttctt cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg
     4021 tcggcggaaa aatgttgctg gtgctaatgg cggtgattgg cattatcgct aacccaaaca
     4081 cagtgccgca gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg
     4141 atacttttta ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac
     4201 agcataaagc actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct
     4261 ctcgcgggac attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt
     4321 actgtgggcc gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc
     4381 tggatacgcg taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg
     4441 gattccacgc cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc
     4501 caatactgga tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta
     4561 tgctggtaca acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt
     4621 ggactgaccg ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt
     4681 ttggcgggtt tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc
     4741 ttaagaagaa agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca
     4801 gaccta
//
LOCUS       DAZOXR010000249         4796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZOXR010000249 DAZOXR010000000
VERSION     DAZOXR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.256"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUNN_004053"
     tRNA            6..81
                     /locus_tag="ACHUNN_004053"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUNN_004054"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUNN_004054"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUNN_004055"
     CDS             complement(299..1057)
                     /locus_tag="ACHUNN_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0578275.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUNN_004056"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUNN_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0578276.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUNN_004057"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUNN_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="ACHUNN_004058"
     CDS             complement(3427..4452)
                     /locus_tag="ACHUNN_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0578277.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAZOXR010000250         4777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZOXR010000250 DAZOXR010000000
VERSION     DAZOXR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.257"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(51..494)
                     /locus_tag="ACHUNN_004059"
     CDS             complement(51..494)
                     /locus_tag="ACHUNN_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0578278.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            651..1580
                     /gene="metA"
                     /locus_tag="ACHUNN_004060"
     CDS             651..1580
                     /gene="metA"
                     /locus_tag="ACHUNN_004060"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0578279.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1849..3450
                     /gene="aceB"
                     /locus_tag="ACHUNN_004061"
                     /pseudo
     CDS             1849..3450
                     /gene="aceB"
                     /locus_tag="ACHUNN_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3480..4771
                     /gene="aceA"
                     /locus_tag="ACHUNN_004062"
                     /pseudo
     CDS             3480..4771
                     /gene="aceA"
                     /locus_tag="ACHUNN_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1189 a         1259 c         1208 g         1121 t
ORIGIN      
        1 tgcgacgctg gcgcgtcttc tccagcctgg gcaggcacgg caggggcagc ttattcctcc
       61 acatacgcca gattcagcaa cggatacggt ttccccaaat cgtccacctc agagcgtccc
      121 gtaaccttaa aacccacctt cttatagaac ccaaccgcct gctcattttg ctcattaacg
      181 ttggttgtca gttccggtgc catcgagagc gcatgctcca ccagcatccg acccacgccg
      241 cagccgcaca catcaggatc gataaacagc gcatccatat gctgcccact taacaacata
      301 aacccaaccg gctgatcccg ttcattaacc gcgacccaca acggcgcttc cggcaggaag
      361 gaacgaacta gcacctccag ctcggcccga tactctgctg ataaaaaatc gtgtgtggca
      421 tcgacagaac gacaccaaat cgcaacgagt tcctcccctt cctcatgccg tgagcggcga
      481 atactaataa ccattttctc tccttttcgt cattcttata ttctaacgta gccttttcct
      541 tgaaactttc tcaccttcaa catgcaggct cgacattggc aaattttctg gttatcttca
      601 gctatctgga tgtctaaacg tataagcgta tgtagtgagg taatcaggtt atgccgattc
      661 gtgtgccgga cgagctaccc gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga
      721 caacttctcg tgcgtctggt caggaaattc gtccacttaa ggttctgatc cttaacctga
      781 tgccgaagaa gattgaaact gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc
      841 aggtcgatat tcagctgttg cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc
      901 atctgaacaa cttctactgt aactttgaag atattcagga acaaaacttt gacggtctga
      961 tagttaccgg cgcgccgctg ggcctggtgg agtttaatga tgtcgcttac tggccgcaga
     1021 tcaaacaggt gctggagtgg tcgaaagatc acgtcacctc gacgctgttt gtctgctggg
     1081 cggtacaggc cgcgctcaat atcctctacg gcattcctaa gcaaactcgc accgacaaac
     1141 tctctggcgt ttacgagcat catattctcc atcctcatgc gcttctgacg cgtggctttg
     1201 atgattcatt cctggcaccg cattcgcgct atgctgactt tccggcagcg ttgattcgtg
     1261 attacaccga tctggaaatt ctggcagaga cggaagaagg ggatgcatat ctgtttgcca
     1321 gtaaagataa gcgcattgcc tttgtgacgg gccatcccga atatgatgcg caaacgctgg
     1381 cgcaggaata tttccgcgat gtggaagccg gactagaccc ggatgtaccg tataactatt
     1441 tcccgcacaa tgatccgcaa aacaaaccgc gcgcgagctg gcgtagtcac ggtaatttgc
     1501 tgtttaccaa ctggctcaac tattacgtct accagatcac gccatacgat ctacggcaca
     1561 tgaatccaac gctggattaa tcttctgtga tagtcgatcg ttaagcgatt cagcacctta
     1621 cctcaggcac cttcgggtgc cttttttatt tccgaaacgc acctcagcaa gtgaataaat
     1681 tttattcata ttgttatcaa taagttatca agtattttta attaaaatgg aaattgtttt
     1741 tgattttgca ttttaaatga gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc
     1801 ttcgttcaca gtggggaagt tttcggatcc atgacgagga gctgcacgat gactgaacag
     1861 gcaacaacaa ccgatgaact ggctttcaca aggccgtatg gcgagcagga gaagcaaatt
     1921 cttactgccg aagcggtaga atttctgact gagctggtga cgcattttac gccacaacgc
     1981 aataaacttc tggcagcgcg cattcagcag cagcaagata ttgataacgg aacgttgcct
     2041 gattttattt cggaaacagc ttccattcgc gatgctgatt ggaaaattcg cgggattcct
     2101 gcggacttag aagaccgccg cgtagagata actggcccgg tagagcgcaa gatggtgatc
     2161 aacgcgctca acgccaatgt gaaagtcttt atggccgatt tcgaagattc actggccccg
     2221 gactggaaca aagtgatcga cgggcaaatt aacctgcgtg atgcagttaa cggcaccatc
     2281 agctatacca atgaagcagg caaaatttat cagctcaagc ccaatccagc ggttttgatt
     2341 tgtcgggtac gcggtctaca cttgccggaa aaacatgtca cctggcgtgg tgaggcaatc
     2401 cccggtagcc tgtttgattt tgcgctctat ttcttccaca actatcaggc actgttggca
     2461 aagggcagcg gtccctattt ctatctgccg aaaacccagt cctggcagga agcggcctgg
     2521 tggagcgaag tcttcagcta tgcagaagat cgctttaatc tgccgcgcgg cgccatcaag
     2581 gcgacgttgc tgattgaaac gctgcccgcc gtgttccaga tggacgaaat ccttcacgcg
     2641 ctgcgcgacc atattgttgg tctgaactgc ggtcgctggg attacatctt cagctatatc
     2701 aaaacgttga aaaactatcc cgatcgtgtc ctgccagaca gacaggccgt gacgatggat
     2761 aaaccattcc tgaatgctta ctcacgcctg ctgattaaaa cctgccataa acgcggtgct
     2821 tttgcgatgg gcggcatggc agcgtttatt ccgagcaaag atgaagagcg taataaccag
     2881 gtgctcaaca aagtaaaagc ggataaagcg ctggaagtca ataacggtca cgatggcaca
     2941 tggatcgctc acccaggcct tgcggacacg gcaatggcgg tattcaacga cattctcggc
     3001 tcccgtaaaa atcagcttga agtattgcgc gaacaagacg cgccgattac tgccgatcag
     3061 ctgctggcac cttgtgacgg tgaacgcacc gaagaaggta tgcgcgccaa cattcgcgtg
     3121 gctgtgcagt acatcgaagc gtggatctcc ggcaacggct gcgtgccgat ttatggcctg
     3181 atggaagatg cggcgacggc tgaaatttcc cgtacctcga tctggcagtg gatccatcat
     3241 caaaaaacgt tgagcaatgg caaaccggtg accaaagcct tgttccgcta gatgctgggc
     3301 gaagagatga aagtcattgc cagcgaactg ggcgaagaac gtttctccca gggccgtttt
     3361 gacgatgccg cacgcttgat ggaacagatc accacttccg atgagttaat tgatttcctg
     3421 accctgccag gctaccgcct gttagcgtaa accaccacat aactatggag catctgcaca
     3481 tgaaaacccg tacacaacaa attgaagaat tacagaaaga gtggactcaa ccgcgttggg
     3541 aaggcattac tcgcccatac agtgcggaag atgtggtgaa attacgcggt tcagtcaatc
     3601 ctgaatgcac gctggcgcaa ctgggcgcag cgaaaatgtg gcgtctgctg cacggtgagt
     3661 cgaaaaaagg ctacatcaac agcctcggtg cactgactgg cggtcaggcg ctgcaacagg
     3721 cgaaagcggg tattgaagca gtctatctgt cgggatggca ggtagcggcg gacgctaacc
     3781 tggcggccag catgtatccg gatcagtcgc tctatccggc aaactcggtg ccagctgtgg
     3841 tggagcggat caacaacacc ttccgtcgtg ccgatcagat ccaatggtcc gcgggcattg
     3901 agccgggcga tccgcgctat gtcgattact tcctgccgat cgttgccgat gcggaagccg
     3961 gttttggcgg tgtcctgaat gcctttgaac tgatgaaagc gatgattgaa gccggtgcag
     4021 cggcagttca cttcgaagat cagctggcgt cagtgaagaa atgcggtcac atgggcggca
     4081 aagttttggt gccaactcaa gaagctattc agaaactggt cgcggcgcgt ctggcagctg
     4141 acgtgacggg cgttccaacc ctactggttg ctcgtaccga tgccgatgca gcggatctga
     4201 tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt accagtgaag
     4261 gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg gcgtatgcgc
     4321 catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg gcgcgtcgct
     4381 ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac tgctcgccgt
     4441 cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag cagcagctgt
     4501 cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc atgtggttca
     4561 acatgtttga cctggcaaac gcctatgcct agggcgaggg tatgaagcac tacgttgaga
     4621 aagtgcagca gccggaattt gccgctatac cttcgtatct caccagcagg aagtgggtac
     4681 aggttacttc gataaagtga cgactattat tcagggcggc acgtcttcag tcaccgcgct
     4741 gaccggctcc actgaagaat cgcagttcta agcaacc
//
LOCUS       DAZOXR010000251         4728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZOXR010000251 DAZOXR010000000
VERSION     DAZOXR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.258"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..738)
                     /locus_tag="ACHUNN_004063"
     CDS             complement(<1..738)
                     /locus_tag="ACHUNN_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0578280.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(879..2912)
                     /gene="metG"
                     /locus_tag="ACHUNN_004064"
     CDS             complement(879..2912)
                     /gene="metG"
                     /locus_tag="ACHUNN_004064"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0578281.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3044..4153
                     /gene="apbC"
                     /locus_tag="ACHUNN_004065"
     CDS             3044..4153
                     /gene="apbC"
                     /locus_tag="ACHUNN_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0578282.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESAFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4398..4679
                     /locus_tag="ACHUNN_004066"
     CDS             4398..4679
                     /locus_tag="ACHUNN_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0578283.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1141 a         1177 c         1182 g         1228 t
ORIGIN      
        1 aatatcaatc tgttgaaggt gtattattac atcagtagca tattccagac cgccttcctg
       61 gacgatctga tccataacat cctcacgcac tttatatctt gtgcatttca tttcaagtgc
      121 cgtgagtaca gccattactg gtgatggtaa ctgtgttgtt ttcaggccgt cgatctgact
      181 gatcgcctgg gcgacggtgt tttgccattc taaagaacag gcgctgaagt caaatgtggc
      241 tgttttatta tcccgttgcg acacactaca ggctaagcga cgtaattggg tcaatttgtc
      301 tgcttgctga acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc
      361 gggcggaaaa acgacagcaa tatctttcgc cagccacggt aaaacagcat ctgtattttt
      421 gtcaggcgca cgggtttctg ctacgaaaga gggttgttcc tgaataatgg tggataagtc
      481 cgctaccttg agagagcccg ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg
      541 ctccacatac cccttcttca ccttctccgc aatcagctta agctccgctt ttgccgctgc
      601 cgcagcatct gaaaaacttt ttatctggct ttgccctttg gtgccaactt ttccccaact
      661 gatatgcaac tcgtttccct gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg
      721 atagataaag tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac
      781 cagtatttat gcgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata
      841 aagaaaaggc gctgtcgatg cagcgccttg aagggggatt atttcacctg atggcctggt
      901 ttagcaccag catccgggct taacaggaag atatctttcc cgccaggacc agcagccatt
      961 accatgcctt cggagatacc aaagcgcatt ttacgcggtg ccaggttggc caccataatg
     1021 gtgtgacgcc cgatcagtgc ctgcggatcc gggtaggcag aacgaatacc ggagaagaca
     1081 ttgcgttttt caccgccgag atccagtgtc aggcgcagca gtttgtcaga accttcaaca
     1141 aactctgcgt tttcgatcag cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg
     1201 atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggc agctgctttc
     1261 acttcttctt tagacgcttc caccagtgct tcaacctgct tcatatcgat gcggttatac
     1321 agcgctttga acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc
     1381 agttctgtat tgaggaatgc ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg
     1441 taagtcatca gcacgcggaa caggtttatg cccattgagc aaattgcctg caggtcggca
     1501 tcgcggcctt cctgttttgc caccacccac ggagcctgct cgtcgacata gcggttagcc
     1561 agatcagcca gcgccatgat ttcgcgcact gctttaccaa attcgcggct ttcccacgct
     1621 tcaccaatca cttcagcggc atcagtgaag gttttgtaca actgcgggtc agccagttcg
     1681 cttgccagca cgccgtcaaa acgcttgttg ataaagcccg cattacgaga ggccaggtta
     1741 accactttgt tcacgatatc ggcattcaca cgctgaacga aatcctccag gttgagatcg
     1801 atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca
     1861 aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga catctttgcg
     1921 ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct
     1981 tccagcatgg caggccagaa caggctgtgg aagtaaacaa tatctttacc gatgaagtgg
     2041 tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct gtcgctgcgc
     2101 ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag
     2161 aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata
     2221 tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tatttgccac ctgctcctgc
     2281 aacgcaccgc tgcgggtcca tgcctgcaac atttcgctga aagagggcag atcaaagaag
     2341 aagtgttcag aatcacgcat taccggcgta gcgccagaaa ccaccgattt cggctcgatc
     2401 aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg
     2461 gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc
     2521 ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttctcttt caggcgagag
     2581 tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag
     2641 ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc
     2701 tccggggtga taccaagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg
     2761 gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc
     2821 tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac
     2881 gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa
     2941 ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt
     3001 aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca
     3061 ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca
     3121 cccaaccctg aagcataacc tcaccacgct gaacgcgttg caccatgtcg cctggatgga
     3181 cgacacgttg catgtggaac tggttatgcc attcgtctgg catagcgcgt ttgaagagtt
     3241 aaaagagcaa tgtagcgccg aactgctgcg tatcaccggg gcgaaagcta tcgactggaa
     3301 actgtcgcac aatatcgcca ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt
     3361 gaagaacatc attgcggtca gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt
     3421 aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat
     3481 ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga
     3541 cggtacccat atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct
     3601 ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca
     3661 gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg
     3721 caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt
     3781 caccaccccg caggacatcg ctttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa
     3841 agtcgaggtt ccggtgcttg gtattgtcga gaacatgagt gtgcatattt gcagtaactg
     3901 tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca
     3961 cacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg
     4021 cacgccaacg gttatcagcc gtccggagag cgcatttacc gctatctacc gccagctggc
     4081 tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attccaggcg agatttcctt
     4141 ccgcgcggtc taacgcctat taaccattct ggttattctt catacaccgc agagatgtta
     4201 cattgatgcg gtgtattagt ttttgccgca ttaatgaaca cttatttaat actgcgaata
     4261 ttctgcggac cttcgtccga aactctacaa agtcgattga tcatacaggc aatgcttcat
     4321 tatcatcctt cgtggctgtg tagtggatgt acagctatag tttgttatgc actcattcta
     4381 tccaggcata accttcaatg aataagtatt ggctgtcagg aattattttt cttgcctatg
     4441 gattggcttc tcccgcattc tcttcagaga ctgccacatt agcgattaat ggcaggatca
     4501 gtcccccaac atgcagtatg gctatggtta atggtcaacc tcaaccgcat tgtggtcagt
     4561 taacgtataa tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt gtaacgacag
     4621 aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc tacgattaat
     4681 ttggcaacct gatttttatt gatatcaaca ataccattta cataaatc
//
LOCUS       DAZOXR010000252         4606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZOXR010000252 DAZOXR010000000
VERSION     DAZOXR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.259"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..2162
                     /locus_tag="ACHUNN_004067"
     CDS             <1..2162
                     /locus_tag="ACHUNN_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0578284.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="ACHUNN_004068"
     CDS             complement(2396..3679)
                     /locus_tag="ACHUNN_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0578285.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..>4606)
                     /locus_tag="ACHUNN_004069"
     CDS             complement(3814..>4606)
                     /locus_tag="ACHUNN_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0578286.1"
                     /translation="QKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKA
                     ASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESS
                     PCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGI
                     SMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELR
                     SLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1177 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgc
//
LOCUS       DAZOXR010000253         4606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZOXR010000253 DAZOXR010000000
VERSION     DAZOXR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.260"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            29..853
                     /gene="rcnA"
                     /locus_tag="ACHUNN_004070"
     CDS             29..853
                     /gene="rcnA"
                     /locus_tag="ACHUNN_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR0578287.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1072..1410
                     /gene="rcnB"
                     /locus_tag="ACHUNN_004071"
     CDS             1072..1410
                     /gene="rcnB"
                     /locus_tag="ACHUNN_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0578288.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1492..2526)
                     /locus_tag="ACHUNN_004072"
     CDS             complement(1492..2526)
                     /locus_tag="ACHUNN_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR0578289.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(2542..>4606)
                     /locus_tag="ACHUNN_004073"
     CDS             complement(2542..>4606)
                     /locus_tag="ACHUNN_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0578290.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
BASE COUNT         1234 a         1128 c          942 g         1302 t
ORIGIN      
        1 tgccgaatta atactaagaa ttattatcat gaccgaattt acaactcttc ttcagcaagg
       61 aaacgcctgg ttcttcatcc ccagcgccat cttacttggt gcgcttcatg gcctggaacc
      121 agggcactca aaaacgatga tggcggcgtt tatcatcgcc atcaaaggca ccattaaaca
      181 agcggtgatg ctcggactgg cagcaactat ttcgcatacc gcagtggtct ggttaattgc
      241 ctttggcggg atggtgatca gcaagcgctt tactgctcaa tcagcagaac cgtggctcca
      301 gctgatttcc gcagtgatca ttattggcac cgcgttctgg atgttctggc gtacctggcg
      361 cggcgaacgc aactggctgg agaatatgca cgagtatgat tatgagcatc atcatcacga
      421 tcacgaagat caccacgacc atggacatca tcaccatcac gaacatggcg agtatcagga
      481 tgcccatgca cgagcccatg ccaatgacat taaacgacgc tttgatggta gagaggtcac
      541 caactggcaa attttgctat ttggcttaac cggtggcctt atcccctgcc cggcagcaat
      601 taccgtgctg ttgatatgca ttcagttgaa agccctgaca ctgggcgcaa cactggtcgt
      661 cagtttcagc attggcctgg cgttaacgct tgtcaccgta ggcgttggcg cagcaatcag
      721 cgttcagcag gtcgcaaaac gctggagcgg atttaacact ctcgctaaac gcgcccccta
      781 tttttccagt ctgttgattg gcttagtcgg tgtgtatatg ggcgtacatg gcttcatggg
      841 cataatgcga taactcaatc ctttaggctt gcatgttatc ttcagcccag gatgattctt
      901 aaatcagcta tttccgctga ctctctgctc gctaaggatg cttttagggc atcctttcat
      961 ttacactttt tacgaaatca tgggatcact aacaaaatat cgcttgtcag ttatattgta
     1021 tggcaggaaa gatatgcgac tgatattaca gatccccaaa gtggagagtt tatgaccatt
     1081 aaaaataaga tgttgctggg tgtgcttttg ctggttacca gtgccgcctg ggccgcacca
     1141 gccaccgcgg gttcgaccaa tacctcggga atttctaagt atgagttaag tagtttcatt
     1201 gctgacttta agcatttcaa accaggtgat accgtcccag agatgtaccg caccgatgag
     1261 tacaacatta agcagtggca gttacgtaac ctgcccgcgc ctgatgccgg gacgcactgg
     1321 acctatatgg gtggcgcgta cgtgttgatc agcgacaccg acggtaaaat cattaaagcc
     1381 tacgacggtg agatttttta tcatcgctaa aaaaagcccc ctcatcatga gggggaaatg
     1441 cagacacctt gttatttttt attattagcc acttgctcgt cttgcttgtt attagtcgta
     1501 tttcacgttg attaatgcgg ttgcctccag tgcgccagat ttaactttgt ttgtatcgta
     1561 gacgtagtaa ctggcagtta tcggaattga aaccgttgta tctgtaagtt ttgactttgc
     1621 cgcaatttct tttaaatttg tgtatccttt gctgttatca gtgatattta aaaatgtgac
     1681 tggagaacca ttgctttcaa gcataaatcc gactccatta ttttctgcac ctttcaggat
     1741 cgtgttactg ccggctaaca aatcagaact tgataaatac acctgcattt tccgtgatgt
     1801 ttgattttgt tgacctaata aatttttaca acgcagaata atatcccctt gtttactatt
     1861 tattgttgct ttgttacctg tggctggtaa ttgtgaaacg ggaatactgt ctaaagtaat
     1921 taataaatcg tccggtttgc aggttgtggt tatcgggttg tatgacaatg tcagattaaa
     1981 ggaatagaca cctgcatttt gtgccaatgc atttctgtag ctatttacac atgcatccca
     2041 tgacttgcca cctaataaac acagtagaaa ttgtactgcg gcactcccgc gtgtcgatga
     2101 tgaagcactg gataatgtcg caacaccatt aatcatcact gaaccgccgc cccccgcaga
     2161 aacattcact tttgcattac tggccgcagg tgtcaccgta actgaataac cactggtgaa
     2221 actggtctga ttatttgaca acgtgatgtt gtttttattg gtccattcaa aatgtaaatt
     2281 taattttatg gtatcgttat aaagcccaac aataatatcc gacgaactca taggggtgat
     2341 agtatttgta ctcacgcatg acgaatcatt aaaattatgc tcatatgtcg ctatttgtgt
     2401 ttgagtaagt gcaaaatccc cctgaacaac caaattgtgc tgaacagata actcactata
     2461 acatgccgca tagctcacag gcataaccat catcattaat ataaatagca ttatgcgaat
     2521 ttccatagaa ccacctgact attactggca aatataattt ctactttcat caatctcttt
     2581 accgaaggtg attgtgcatg aaagtccttg ttgcttatca attgctacat taaccgatgg
     2641 cggtatttca ttggtgcgaa taaataactg gcttccctgg ccgacaacgc caatattatg
     2701 accgtggata tcattgactt cataaccaaa cgttaatggt tgcccatccg ctcttaacgc
     2761 ttttataaac cagggcttgc gctgatcggt atcaaaatta accagtacaa ccgcgccgcg
     2821 ataaggtgcg gcaattttcc ggttgccacg taattctgct tcgctatcgc tttgcgacac
     2881 atccagcatc aggtgatttt cccgataagg tgtcattccg tcgtatacca ccactccatt
     2941 acggtttgtt gtgcgatatt tttgcccatt gacataagca tctttaattc ctggcgcatt
     3001 catcacagca aacgtttcgg aaagacggtt cgccagatta acgccacccg accaggcgac
     3061 aatgccccct gaaacactgg ctccagcctg tcgataagta ctcgactgac tataactgcc
     3121 attcactgtt gccaccggcg cgttccaggt taaattcgct ccagctgtcg tttcatttcc
     3181 ctgatgttga tgactcaggt tgacaccata attgaactga tcccgactcc ctactgttcc
     3241 tgataatccc gtattatttg aggcaaaccc ctgatcatca aacgtcgttg agttagacat
     3301 atatatttgc cgacgaggcg tcgaaacgtc atcaccccaa tcaaagggaa tcgatataaa
     3361 aatattaaaa cgtttctctt catgatgatt ctcgtcataa gcctggcttg ccgcgagggt
     3421 atagcttatc cgtcgcaggt tgttggaata actcaactga taatccttac tactgccgct
     3481 acgcccccag taatctcgcc ataacgtact taatgacaca gacccccaac cttctggcaa
     3541 tgactggctc atattggcgg aaaagctatt tttgcgacca aaatcattct ggtaataatc
     3601 ggcaatgtca tagacatcgt tttcatcacg gcgataatta tctttattgt ttgcccaaac
     3661 gtgatcgtta aatgtccggt aatcacgcga cgaataacgc caggccgcca gaccaaaacg
     3721 cgtcgacgtt tggctcacaa atttgttgta ggcaatttga taactttgcc cgtcaaacac
     3781 atcgccgttg tcttgtttac tatgcgactt cgtggcatcg acggaaatgg caccaatgcg
     3841 tgtattccag ccagtcccca aagtgaacgc gtaataatta ttcgcaacca tcgagccacc
     3901 atacaacgtc aataagttat taaaaccata ctgataaccc gcctggacaa aatcactatg
     3961 tttgctcgcc ccttcaatat ggctacgacc cgccgcaaaa tcatatttcg acacgccggg
     4021 ttgcagcata tttggcaccg ctgcataagg caccagatag gtggttaccg aaccgtccgc
     4081 ctctttcacg ctgacatcaa gatctgctcc accaccggcc aactgcaaat ctgtaatcgc
     4141 aaacgggcca ggaggaacct ctttctgata aaccacaaaa ccattctgtt caatagttac
     4201 cagcgcgtta ctctgagcaa tcccctgcac ccgtggcgta aaattttgtt tcgagttagg
     4261 caacatctgc atatcacgaa acaaccgcac acctctgaag cgaacagaat caaaaatatc
     4321 gcttgatgtg tacatatcac ccacgcgaag cgtgccgaga agttgggcaa atccacgttc
     4381 cagatacagg gtattgcttt tccacacccc tggattgtta tttgttttac tgaaactggc
     4441 atcagaatgc agttgccacc ccagtaaatt taacccgctg ttaaaacgta catatgtact
     4501 cttactatta cccgacgctt tatagtcact gtaatactga ctcacataat aagaggtata
     4561 aaacgcatta ataccccgct cccagttttc cggtggaaca tagcca
//
LOCUS       DAZOXR010000254         4528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZOXR010000254 DAZOXR010000000
VERSION     DAZOXR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.261"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUNN_004074"
     CDS             complement(139..3654)
                     /locus_tag="ACHUNN_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0578291.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4528)
                     /locus_tag="ACHUNN_004075"
     CDS             complement(4106..>4528)
                     /locus_tag="ACHUNN_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0578292.1"
                     /translation="ESANYIGARSVTAAIKPDIKLQPTRHVKTMDALQTGCVHINTVN
                     KALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKA
                     EHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1167 a         1158 c          933 g         1270 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acagtattta tatgaacgca tcccgtttgc aaggcatcca
     4441 ttgttttgac atgacgggta ggttgcagct ttatatccgg ctttattgca gccgtaacgc
     4501 tgcgagcccc tatgtaattc gccgactc
//
LOCUS       DAZOXR010000255         4483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZOXR010000255 DAZOXR010000000
VERSION     DAZOXR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.262"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHUNN_004076"
     CDS             complement(8..1069)
                     /locus_tag="ACHUNN_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0578293.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHUNN_004077"
     CDS             complement(1066..2463)
                     /locus_tag="ACHUNN_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0578294.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHUNN_004078"
     CDS             2618..3616
                     /locus_tag="ACHUNN_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0578295.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHUNN_004079"
     CDS             complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHUNN_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR0578296.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1064 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaattgatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc gcc
//
LOCUS       DAZOXR010000256         4471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZOXR010000256 DAZOXR010000000
VERSION     DAZOXR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.263"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..268)
                     /locus_tag="ACHUNN_004080"
     CDS             complement(<1..268)
                     /locus_tag="ACHUNN_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0578297.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPM"
     gene            497..1105
                     /locus_tag="ACHUNN_004081"
     CDS             497..1105
                     /locus_tag="ACHUNN_004081"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0578298.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1203..2594
                     /gene="selA"
                     /locus_tag="ACHUNN_004082"
     CDS             1203..2594
                     /gene="selA"
                     /locus_tag="ACHUNN_004082"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0578299.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2591..4435
                     /gene="selB"
                     /locus_tag="ACHUNN_004083"
     CDS             2591..4435
                     /gene="selB"
                     /locus_tag="ACHUNN_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0578300.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1001 a         1139 c         1322 g         1009 t
ORIGIN      
        1 ccatcggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat
      121 tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccaca ccggtggggg
      181 caccgatgcg caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc
      301 tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata tccatcagat
      361 agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa
      421 caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc
      481 gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact
      541 ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa
      601 cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga
      661 agagggcgaa tgctggtttg attcgccgat catcgctgaa tatattgagt taatgaatgt
      721 cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc
      781 gctggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccggc
      841 ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga
      901 tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac
      961 tatcgccatt gcctgtgctg tcggatatct caatttccgc cgcgttgcgc caggttggtg
     1021 tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc
     1081 tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac
     1141 aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc
     1201 ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc
     1261 gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt
     1321 tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtga cagccagacg cttcctgcgt
     1381 ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaggcg cagagcgcgc
     1441 tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttgga cgagctttac
     1501 aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt
     1561 acgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa ctgctgtgcc
     1621 gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga
     1681 tgttggcggc cactgccagc gggaaagagg tggtagtatc tcgcggcgaa ttggtggaga
     1741 ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag
     1801 tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg
     1861 cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag
     1921 atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag
     1981 gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg
     2041 agttgattgc ggcgggcgtc agtctggtga gcttctccgg cgacaagttg ttaggcgggc
     2101 cgcaggcagg gattatcgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc
     2161 tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc
     2221 tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca
     2281 gcgcagaggt cattcaaatc caggcacaac gtttacaggc tccccttgcc gcacattacg
     2341 gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc
     2401 cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc
     2461 accttgagtc attagccgcc cgctggcgtg aattgccagt gccgatgatt ggtcgtattt
     2521 atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga
     2581 tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt
     2641 gcaggtgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac
     2701 catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgccta gttttatcga
     2761 cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca
     2821 cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg agcatctggc
     2881 gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt
     2941 ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg
     3001 ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct
     3061 gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc agccaacata gtgtccgcct
     3121 cgcgatcgac cgcgcattta ccgtaaaagg tgccggtctg gtcgtcaccg gtacggcgtt
     3181 aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg
     3241 tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat
     3301 cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct
     3361 tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc
     3421 gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt
     3481 ttcgctgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga
     3541 taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt
     3601 ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc
     3661 gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc
     3721 ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt
     3781 gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc
     3841 ccgctggcag cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga
     3901 acctggtcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct
     3961 ggtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc atcacggttg
     4021 gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa
     4081 agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg
     4141 taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac
     4201 cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat
     4261 ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg
     4321 cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg
     4381 tcgtcgtgga aatgatcatt tattgcgcga cgcattatta tttccggaaa aataaggaaa
     4441 tgattaatta atttttaaaa taaattaata c
//
LOCUS       DAZOXR010000257         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZOXR010000257 DAZOXR010000000
VERSION     DAZOXR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.264"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUNN_004084"
     CDS             complement(<1..105)
                     /locus_tag="ACHUNN_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578301.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUNN_004085"
     CDS             complement(173..2122)
                     /locus_tag="ACHUNN_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0578302.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUNN_004086"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUNN_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0578303.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUNN_004087"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUNN_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0578304.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUNN_004088"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUNN_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0578305.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1314 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZOXR010000258         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZOXR010000258 DAZOXR010000000
VERSION     DAZOXR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.265"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(21..1967)
                     /locus_tag="ACHUNN_004089"
     CDS             complement(21..1967)
                     /locus_tag="ACHUNN_004089"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0578306.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SADMTFRVKPESLILTIFFLGLIP"
     gene            complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUNN_004090"
     CDS             complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUNN_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0578307.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2369..3076
                     /locus_tag="ACHUNN_004091"
     CDS             2369..3076
                     /locus_tag="ACHUNN_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0578308.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="ACHUNN_004092"
     tRNA            3182..3257
                     /locus_tag="ACHUNN_004092"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="ACHUNN_004093"
     CDS             3455..>4409
                     /locus_tag="ACHUNN_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0578309.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1057 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZOXR010000259         4407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZOXR010000259 DAZOXR010000000
VERSION     DAZOXR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.266"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUNN_004094"
     CDS             complement(<1..590)
                     /locus_tag="ACHUNN_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0578310.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHUNN_004095"
     CDS             complement(583..993)
                     /locus_tag="ACHUNN_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR0578311.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHUNN_004096"
     CDS             complement(999..1532)
                     /locus_tag="ACHUNN_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0578312.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2598)
                     /locus_tag="ACHUNN_004097"
     CDS             complement(1510..2598)
                     /locus_tag="ACHUNN_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR0578313.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2595..3986)
                     /locus_tag="ACHUNN_004098"
     CDS             complement(2595..3986)
                     /locus_tag="ACHUNN_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR0578314.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4033..>4407)
                     /locus_tag="ACHUNN_004099"
     CDS             complement(4033..>4407)
                     /locus_tag="ACHUNN_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578315.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1056 a         1286 c         1041 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc gccgtcgccg ctttacctgt tccgggtttt cccagaacca gtcgcccgca
     2101 ttcatcgtca taagccagcg tctgaacctg ccccagtaac cggttaagac aatcggcaac
     2161 ggtttcgccg tgttccggct gggcatcaat caccgctgtc tgcggcacgc cagcatcaac
     2221 aacggtgatg ccaaatggcg cagccagttc actgactatt ctgagcaggt tttttccgct
     2281 ctgctggagt ggcaaagcag agcagtcaac cagatcggct gttttgcttc gcccgacaat
     2341 tcccatgctg acgctgctgg cgtcataacg aagcggtagt gcctccacat atccggtgag
     2401 cacaggctca tccccgataa gcacttcaac cagctcacca ttttttatcc ggggttgata
     2461 atcccggctt ccgggccagc gggtggtaat ggcaacatta aaatcccggg caatacggtt
     2521 aatgcccgca ctgatacgga cggatgtcca gccgccccat tcgcgaccgg aaacccgaag
     2581 taaaacggta tcattcatct gacgggtacc cttaatgccc tgaccggaac aaagcccgga
     2641 tgagagatgg catttcgatc caggatatca gtttcacggg acgcatcgtc gtaccatgac
     2701 gcagccagaa caagcgcagg cagaacttcc gctggcgttc gctctgcggt ttcctccgtc
     2761 tgaaccagac gcgcctgaat atcccggttc agttctgtac gtaatgacgt cagctgaaaa
     2821 aacagcctgt catccgccgt acgcctgagc tcctgttcaa tcgccgcgtt cagcgatttg
     2881 cgtatgatgg tgagattttc ccgcgtgggt ggttgcgctc tctcatcctg tcctgtgctg
     2941 gctgccacgc tgtcaagtgc cggatgagaa acgtgaataa tatcggactg acgttcagtg
     3001 gaaccaccaa cagccacaac cgcctgctga tttttcacca ggcttccggg ttgtggcagt
     3061 gaagtgaccg cccttgtggc ttcgctgatt gccgtcgtcc ggatgacagc agccacaagg
     3121 tttgtctgct gtttttgcct gataaccgat gcggaatctg tgggccacac cgcacgcggt
     3181 gccagtcctg ggtccagcgt aatgccggac attgtcgtta tggactgcac cagatcctgt
     3241 gtgttatcca ccagccgggt tccggcccgc caggtatcct gcaacgtatg cacaaaatca
     3301 ctggcaaccg acggcggcat cagaatgacg gataaatcgc cctgcagtag tctcgctccg
     3361 gcagaaacag ccgaattaac cattttgaaa gctgtctgaa cggttcccag catatcggtc
     3421 gtccgggcaa taacatcgtt ctgaataaag tccggtattc ccgcgagatc aaaatcgccg
     3481 aacatgtctt caatcaactc atccaggaat acggacgact cctccagttt tctggcggtt
     3541 gcagctccgg cgaccggaaa tgacagttcc ccgctctcaa caaactgaaa tgaaacccgg
     3601 cacatacggc cttcagtgcc ggagtgagaa acggtcacct gcccgtcaat acagccctgc
     3661 atttcaccga actgcggatg gatcagcgtc cccggcccgg cggtttcaat cgctgtaatc
     3721 agcctgtcgc gctgctctgc gtaatcatca ccaacgagat acgcattaat cgtcagccgt
     3781 cgcgtggcac gcccgagatc ttccgtgtac ggtttgtcac ggtttgggta ttcatggacc
     3841 tgaacgcggc gtccgaacga accctcgtcg ctctccaccg aaaacggaac gccacgaaat
     3901 gaagcgtcgt aaagattatc gcgccaattg atacctgaag atgcagaaaa gagtgaagaa
     3961 agagaaggta aagaaggtaa gtccatactg tctccactat aaaaaaaccg ccaatttctg
     4021 gcggcttata tattaatcat cattaatatc tttcatattc aatttgcaat tttcgtaggt
     4081 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
     4141 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
     4201 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
     4261 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
     4321 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
     4381 caagtcaacg tcattatctg cagatgt
//
LOCUS       DAZOXR010000260         4367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZOXR010000260 DAZOXR010000000
VERSION     DAZOXR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.267"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            56..1741
                     /locus_tag="ACHUNN_004100"
     CDS             56..1741
                     /locus_tag="ACHUNN_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0578316.1"
                     /translation="MNTALSIMGDASSNTAIDYRQEMNVIHEIVAECEKEISFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRVWLDNVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWRELFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKPGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            1803..2507
                     /locus_tag="ACHUNN_004101"
     CDS             1803..2507
                     /locus_tag="ACHUNN_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578317.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GDFDESAKTFIDFIAKRWSEQWKDLEKRANEFDRFMDTMDTAKEALAAGTPLDLESLF
                     NSTMASEMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQREEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            2507..2887
                     /locus_tag="ACHUNN_004102"
     CDS             2507..2887
                     /locus_tag="ACHUNN_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578318.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMSNQPILDGHIQMVINSHEKGWAAIVPCTITYRLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILYGN"
     gene            3026..3568
                     /locus_tag="ACHUNN_004103"
     CDS             3026..3568
                     /locus_tag="ACHUNN_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578319.1"
                     /translation="MSNKIENPVVLIHKRENHDSYEVAITNGSHDYYDGLLMASVSPD
                     EADNSFAVFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDAMCAEEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGK
                     VEMTEWLVDFAAQLRKGGNQ"
     gene            3565..3819
                     /locus_tag="ACHUNN_004104"
     CDS             3565..3819
                     /locus_tag="ACHUNN_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578320.1"
                     /translation="MSNSARLQLGFSPLSKTIMLAKMRDVEGGRMRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEIVWELDDGSRMVLKAEKQEAASERN"
     gene            3806..4336
                     /locus_tag="ACHUNN_004105"
     CDS             3806..4336
                     /locus_tag="ACHUNN_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR0578321.1"
                     /translation="MSEINYQALREVAERAIPAMERLLMLPADDDLLSEQELKDSGVD
                     IDALNAFKFLAGPETVLALLDEINALEETRINDVCRIAELTKQLELAKSKLNEQREYY
                     EGVISDGSKRIAELESDSQAQKLVEAIIVAIENEQERLFDEDYLMDSKECIDVIREEV
                     KRWNDSRAAGIRIKGE"
BASE COUNT         1230 a          935 c         1138 g         1064 t
ORIGIN      
        1 ttttattttt gaaataattg catcattaaa caaatcattt cttaatggtg caattatgaa
       61 tacagccctt tccatcatgg gcgatgccag ctcaaacact gctatcgact atcgacagga
      121 aatgaacgtc atccacgaaa ttgtggccga gtgcgagaaa gagatctcct tcatgtatca
      181 ggtacacgac ttcgtttatg gcgatgaacg ccacaacatg attaatcgcc tgcttcgact
      241 aaaccaccga ccagatgaag atcgctcacg tttgaatcga gtctggctgg ataacgtcga
      301 tctggaatgg gtgaaacaga atatttgggc cgagtactgg aggaaggtta cggatatgac
      361 taacgttctg ctgatcatgc cagcttcccg tcgcgacgag tggcgtgagc tatttatcga
      421 gggcaagcag gaagtcatca aaactgacag aaccggctat cagatgaagg ttaaagagtt
      481 cgttggtgta ccggagttca aagcagaaac ggtcatacca acgatgctta atttgctgaa
      541 tgacaggcac aaatatctct ctgagcgcgt gtatggcttg tttaaggcgc tgagtcctgc
      601 gcacaagaca aataagacaa acggtttcag cgagcgtctg ataatcgctg actgcatttc
      661 tgatttctgg cgtgacagcg ttagcgtgaa ctatcgcaaa gaggactaca tcgacgatct
      721 gcgtgtcttg cttcatttct tcgcgcacaa agaatttatt accatcaacc gcactgctga
      781 ggtgctatca gctgcgtatc gggcaaacga ctgccagacc ggtgactgga tgaacgtcga
      841 tggaaatctg atgcgcgtga agatgttcaa gaacggcaac gttcactttg aaatacatcc
      901 tgacgtggcc tggaagttga atgaggtgct ggcttacagt atgcctgctg caatccccgc
      961 gccatgccga actgcgccaa aaacacgggc accaaagcag ttcgggttaa tccagaagac
     1021 gatctccgtg ccggttcgca ctgcgcttcg tgacgggcga ttgagcaaag acaaaggcgt
     1081 atggtacttc tctgattcag ctctccagaa gtcgcaggtg gaagagcttg agcgcacact
     1141 gagcttcatt ggcggcgtgc aggagaaaaa gcactggcag ttcccgtatg agatcggcca
     1201 tacgctaaat acgattgtgg ctaccggttt aataccggat acaaaatcac accagttcta
     1261 ccctacccca cgcttgattg ctgagtacgt tgccagagcc actgaattga agcctggtga
     1321 gaagctgttg gagcctcaag ccggacgtgg ggatcttctg gcctatatta acgccgatct
     1381 ggaagatgtt acctgcatag aaatcgcacc tctcttcgct gatatcctgc gtggaaaagg
     1441 gtatacgaac acgatttgct gcgacttcat aaagtggtct gaggacaacg caggttatca
     1501 gttcgacaaa atcgttatga acccgccgta ctcgcttggt cgtcatagag agcacacgct
     1561 ggctgcgctg gggcatctga aagtcggcgg gcgtcttgta gcagtattgc cgggcactgc
     1621 gccaatactg gactggatga cgatggataa ttacgtttat gccagaggga agtcgtttac
     1681 caacgagttt gaagacacag ggatcacagt cagcgtatac gttttcaaac gcgttaaatg
     1741 ataggtaaat acttacttaa tttgtgtaag aatgtaataa ctaaacgata agagaaaaac
     1801 atatgaacaa cctccagtta gagcatttta acgtcacagg ccattctgat tttcctttca
     1861 agtttacatt gaaaggttat gcagaggatg cggtaggcca gatcattatt gataaaggca
     1921 tcgttaagtt tgagggggat tttgatgaat ccgcgaaaac attcatagac ttcattgcca
     1981 aacgttggag cgagcaatgg aaagacctgg aaaagcgcgc taatgagttt gatcggttca
     2041 tggatacaat ggatacagca aaagaggctc tggctgccgg gactccgtta gatctggagt
     2101 cacttttcaa cagcacaatg gcctctgaga tgttcgccac catgttcgcg ggtgagttcg
     2161 tccgcagcgg tgccaaaaac taccttgagc tggattacaa cgtccctgca attggcgatt
     2221 tcgtcgttac catccaacgc gaagaaggta agacgccagg tgaacgcgtc gcagaacttg
     2281 aggctgttgt ggatcagcgt aacggagagt gtgaccgttt gatcaacgag cttcatgcac
     2341 ttcgggaaga aagaatatgc gcgggtagca atacacgtaa tgctgcggat atctacttcc
     2401 agttagttga ggaatgccag attccaccag gtggctctct tgtcgattac gtcagacatt
     2461 taatggcgga agttaactcc agccataaag atggtgaggt gcgctgatgt ttggcattga
     2521 cgcacagcgt atagccgctt ttgcaaaaag ccctcttgat aatcccttgt ctcgtagtga
     2581 gcaaatggag ctggcaaggc tttttcttca cattcaaaaa caggcagaca ttttcaataa
     2641 catgtctaat caacctattc tggatggtca catccagatg gtcattaaca gtcatgagaa
     2701 aggctgggct gcaatcgtcc cctgcacaat tacatacagg ttggcgaaag aggttcaaga
     2761 gtttcgtaaa gccagtgtcg aatcggagtc taccaaagcc gcaataaaca ctcttattcg
     2821 catggggttc acatggaccg gtggagccta ctggcaagca cctcacccca ttttatacgg
     2881 caattagacg ctacaaatcc ttcggcatga ataaataaag gccataagtt gtggccttaa
     2941 ataaattgtt ttctacattt tcttatttgt gaaaataaac cagcacttgg tagtgcttat
     3001 gtaacagcaa agaagagaaa aacacatgag taacaaaatc gaaaaccccg tagttctcat
     3061 tcacaagcgt gagaaccacg actcctatga ggtggcgatc accaatggga gtcacgatta
     3121 ttacgatggt ctgctaatgg cctcagtgtc gcctgatgag gcagacaact cttttgccgt
     3181 cttcgctatg gttggttact acatggctgc cgaaattgag aagttgcggg cgcagagaga
     3241 cgcattagcg gcggagaatg cagccctgaa agaatctgag cgagcattcg atgcgatgtg
     3301 tgccgaggaa cacggagata attgggttag cgaattaacg gagactccag ccaccgatgc
     3361 ttttctggct gaagtacgtg cgcagggggt ggatgctgct atagaagctg caaaaaatct
     3421 ggtggcccaa gaatatgagt ataaggattt caaagcggcg cagagtgatt gctgtatgca
     3481 ccctggttca gacctggtag ggaaggttga aatgactgag tggttagttg actttgctgc
     3541 ccagcttcgc aaaggaggca accagtgagt aattcagcac gactacagct tggtttttca
     3601 ccgctatcaa aaactatcat gctggcaaaa atgcgcgatg ttgaaggtgg acgtatgcgc
     3661 gttggcaatg atccaggtcg tgatgttacc aatgaggctg ctcaattggt gtggcaactg
     3721 gtcatggctg aaggtggtga gatagtgtgg gagctggatg atggttctcg catggtgttg
     3781 aaggcagaaa agcaggaggc agccagtgag cgaaattaat taccaggcac tgcgtgaggt
     3841 ggcggaacgt gcaattccag caatggaacg cctgttaatg ttgccagctg atgatgactt
     3901 gttaagtgaa caggaactta aagatagcgg tgttgatatt gatgccctca acgccttcaa
     3961 atttctggcc ggaccagaaa ccgtgctggc actactggat gagatcaacg cattagagga
     4021 aacgcgtatc aacgatgttt gccgtattgc ggaactaaca aaacaactgg agttggcaaa
     4081 atcaaaactc aacgagcagc gtgagtatta cgagggcgtt atctcggatg gaagtaagcg
     4141 catagcagag ttagaaagtg attctcaggc acaaaagtta gttgaagcaa tcattgttgc
     4201 gatagaaaac gaacaggaac gtctttttga tgaagattac ctaatggatt cgaaagaatg
     4261 cattgacgta attcgtgaag aagtaaagcg atggaatgat tcccgcgccg ctggcattcg
     4321 catcaaagga gagtgagatg accacatcgc attctgcaat tacccag
//
LOCUS       DAZOXR010000261         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZOXR010000261 DAZOXR010000000
VERSION     DAZOXR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.268"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            147..3239
                     /gene="ebgA"
                     /locus_tag="ACHUNN_004106"
                     /pseudo
     CDS             147..3239
                     /gene="ebgA"
                     /locus_tag="ACHUNN_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            3236..3685
                     /locus_tag="ACHUNN_004107"
     CDS             3236..3685
                     /locus_tag="ACHUNN_004107"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0578322.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKMVLKVTIEDGYFHNK"
     gene            3748..>4330
                     /locus_tag="ACHUNN_004108"
     CDS             3748..>4330
                     /locus_tag="ACHUNN_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0578323.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
BASE COUNT         1044 a         1167 c         1089 g         1030 t
ORIGIN      
        1 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgtcactttt
       61 tagtaaaaat tttactaaac tccccagcaa ttacacaaac taccatcacc atgaatggtt
      121 ccgatttctc tctaccggga ggccctatga atcgctggga aaacattcag ctcacccacg
      181 aaaaccgact tgcgccacgt gcgtactttt tttcatatga ttctgttgcg caagcgcgta
      241 cctttgcccg cgaaaccagc agcctgtttc tgcccttaag cggtcagtgg aatttccact
      301 tttttgacca cccgctgcaa gtgcctgaag ccttcacctc tgagttaatg gctgactggg
      361 ggcatattac cgtccccgcc atgtggcaaa tggaaggtca cggcaaactg caatataccg
      421 acgaaggttt tccgttcccc atcgatgtgc cgtttgttcc cagcgataac ccaaccggtg
      481 cctatcaacg tattttcacc ctcagcgacg gctggcaggg taaacagacg ctgattaaat
      541 ttgacggcgt cgaaacctat tttgaagtct acgttaacgg tcagtatgtg ggtttcagca
      601 agggcagtcg cctgaccgca gagtttgaca tcagcgcgat ggttaaaacc ggcgacaacc
      661 tgttgtgtgt gcgcgtgatg caatgggcgg actctaccta cgtggaagac caggatatgt
      721 ggtggtcagc ggggatcttc cgcgatgttt atctgatcgg aaaacaacta acacatatta
      781 acgatttcac cgtgcgtacc gactttgacg aagcctattg cgatgccacg ctttcctgcg
      841 aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac gacgctggaa tataccctgt
      901 ttgatggcga acgcgtggtg cacagcagcg ccattgatca tttggcaatt gaaaaactga
      961 ccagcgccag ctttgctttt actgtcgaac agccgcagca atggtcagca gaatcccctt
     1021 atctttacca tctggtcatg acgctgaaag acgccaacgg caacgttctg gaagtggtac
     1081 cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg tctgttctgg atcaataacc
     1141 gttatgtgat gctgcacggc gtcaaccgtc acgacaacga tcatcgcaaa ggccgcgccg
     1201 ttggaatgga tcgcgtcgag aaagatctcc agttgatgaa gcagcacaac atcaactccg
     1261 tgcgtaccgc tcactacccg aacgatccgc gtttttacga actgtgtgat atctacggcc
     1321 tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt tgctaatgtc ggcgatatca
     1381 gccgtattac cgacgatccg cagtgggaaa aagtctacgt cgagcgcatt gttcgccata
     1441 ttcacgcgca gaaaaaccat ccgtcgatca tcatctggtc gctgggcaat gaatccggct
     1501 atggctgtaa catccgcgcg atgtaccacg cagcgaaggc gctggatgac acgcgactgg
     1561 tgcattacga agaagatcgc gatgctgaag tggtcgatat tatttccacc atgtacaccc
     1621 gcgtgccgct gatgaatgag tttggtgaat acccgcatcc gaagccgcgc atcatctgtg
     1681 aatatgctca tgcgatgggg aacggaccgg gcgggctgac ggagtaccag aacgtcttct
     1741 ataagcacga ttgcattcag ggtcattatg tctgggagtg gtgcgaccac gggatccagg
     1801 cacaggacga ccacggcaat gtctggtata aattcggcgg cgactacggc gactatccca
     1861 acaactataa cttctgtctt gatggtttga tctattccga tcagacgccg ggaccgggcc
     1921 tgaaagagta caaacaggtt atcgcgccgg taaaaatcca cgcgcgggat ctgactcgcg
     1981 gcgagttgaa agtcgaaaat aaactgtggt ttaccacgct tgatgactac cccctgcacg
     2041 cagaggtgcg cgccgaaggt gaaacgctcg cgacgcagca gattaaactg cgcgacgttg
     2101 cgccgaacag cgaagccccc ttgcagatca cgctgccgca gctggacgcc cgcgaagcgt
     2161 tcctcaacat tacggtgacc aaagattccc gcacccgcta cagcgaagcc gggcattcta
     2221 tcgccactta tcagttcccg ctgaaggaaa acaccgcgca gccagtgcct ttcgcaccaa
     2281 ataatgcgcg tccgctgacg ctggaagacg atcgtttgag ctgcaccgtt cgcggctaca
     2341 acttcgcgat caccttctca aaaatgagtg gcaaaccgac atcctggcag gtaaatggcg
     2401 agtcgctgct gacccgcgag ccaaagatca acttcttcaa gccgatgatc gacaaccaca
     2461 agcaggagta cgaagggttg tggcagccga atcatttgca gatcatgcag gaacatctgc
     2521 gcgactttgc cgtagaacag agcgagggtg aagtgttgat catcagccgc acggttattg
     2581 ccccgccggt gtttgatttc ggaatgcgct gcacctacat ctggcgcatc gctgccgatg
     2641 gccaggttaa cgtggcgctt tccggcgagc gttacggcga ctatccgcac atcattccgt
     2701 gcatcggttt caccatggga attaacggcg aatacgatca ggtggcgtat tacggtcgtg
     2761 gaccgggcga aaactacgcc gacagccagc aggctaacat catcgatatc tggcgcagca
     2821 ccgtcgatgc catgttcgag aactatccct tcccgcagaa caacggtaac cgtcagcatg
     2881 tccgctggac ggcactgact aaccgccacg gtaacggtct gctggtggtt ccgcagcgcc
     2941 caattaactt cagcgcttgg cactatacct aggaaaacat ccacgctgcc cagcactgta
     3001 acgagctgca gcgcagtgat gacatcaccc tgaacctcga tcaccagctg cttggcctcg
     3061 gctccaactc ctggggcagc gaggtgctgg actcctggcg cgtctggttc cgtgacttca
     3121 gctacggctt tacgttgctg ccggtttctg gcggagaagc taccgcgcaa agcctggcgt
     3181 cgtatgagtt cggcgcaggg ttcttttcca cgaatttgca cagcgagaat aagcaatgag
     3241 gatcatcgat aacttagaac agttccgcca gatttacgcc tctggcaaga agtggcaacg
     3301 ctgcgttgaa gcgattgaaa atatcgacaa cattcagcct ggcgtcgccc actccatcgg
     3361 tgactcattg acttaccgcg tggagacaga ctccgcgacc gatgcgctat ttaccgggca
     3421 tcgacgctat tttgaagtgc attactacct gcaagggcag caaaaaattg aatatgcgcc
     3481 gaaagagaca ttacaggtag tggaatatta tcgtgatgaa actgaccgtg aatatttaaa
     3541 aggctgcgga gaaaccgttg aggtccacga agggcaaatc gttatttgcg atatccatga
     3601 agcgtatcgg tttatctgca ataacgcggt caaaaaaatg gttctcaaag tcaccatcga
     3661 agatggttat ttccataaca aataacaact acggcggcaa aaggagtttg ccgccaccgc
     3721 taccctactc attttcggag atgtgttatg tctgatacca aacgtaatac aatcggcaaa
     3781 ttcggcttgc tctcgctgac ttttgccgcc gtttacagct ttaacaacgt tatcaataat
     3841 aatattgatc ttggactggc ctcggcaccg atgtttttcc tcgcgacgat tttttatttt
     3901 attcccttct gtctgatcat cgcagaattt gtttcgttaa ataaaaactc agaagccggt
     3961 gtctacgcat gggtaaaaag ttcgctgggc ggacgttggg catttattac tgcctatacc
     4021 tactggttcg taaacctgtt ctttttcacc tcactgttgc cgcgcgttat tgcttatgct
     4081 tcgtatgcct tcctcggata cgaatatatt atgacgccgg ttgccaccac cattatcagt
     4141 atggtgctgt tcgccttctc cacctgggtt tccaccaacg gggcgaaaat gttggggcca
     4201 attacctccg tcacttcaac gctgatgctg ctgttaacgc tctcctacat tttactggca
     4261 ggtacggcgc tggttggcgg cgtacagcct gctgacgcca tcaccgttga cgcgatgatc
     4321 ccgaacttca
//
LOCUS       DAZOXR010000262         4311 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZOXR010000262 DAZOXR010000000
VERSION     DAZOXR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.269"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            185..337
                     /gene="hokA"
                     /locus_tag="ACHUNN_004109"
     CDS             185..337
                     /gene="hokA"
                     /locus_tag="ACHUNN_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0578324.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(314..385)
                     /locus_tag="ACHUNN_004110"
     CDS             complement(314..385)
                     /locus_tag="ACHUNN_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578325.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(525..737)
                     /gene="cspA"
                     /locus_tag="ACHUNN_004111"
     CDS             complement(525..737)
                     /gene="cspA"
                     /locus_tag="ACHUNN_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0578326.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1018..1308)
                     /locus_tag="ACHUNN_004112"
     CDS             complement(1018..1308)
                     /locus_tag="ACHUNN_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR0578327.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1742..2452
                     /locus_tag="ACHUNN_004113"
     CDS             1742..2452
                     /locus_tag="ACHUNN_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0578328.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2502..3476)
                     /gene="ghrB"
                     /locus_tag="ACHUNN_004114"
     CDS             complement(2502..3476)
                     /gene="ghrB"
                     /locus_tag="ACHUNN_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0578329.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3580..4239)
                     /locus_tag="ACHUNN_004115"
     CDS             complement(3580..4239)
                     /locus_tag="ACHUNN_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR0578330.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1073 a         1036 c         1052 g         1150 t
ORIGIN      
        1 gagaagaaac aaacttatta agctagaata gccacgggtg cttgagactg tttgtctcag
       61 gtattcaccg aaaggcagac agagaaaagc cccacctgac tataaatcaa agtgaggcta
      121 ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac acaggaggct
      181 gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca cgcttttatt
      241 tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg agagttatga
      301 gctggcggca tttttagcct gcaatttgaa agagtaagag tctttggcgg gaagttattc
      361 ccgcctttct tacggcgttg cgcattctca ttgcacccaa atttattctt cacaaaaata
      421 atactaaatt ttattacgcg atcgattatt tatttcctga aaaacaaata aaaaaatccc
      481 cgccaaatgg cagggatctt agattctgtg cttttaagca gagattacag gctggttacg
      541 ttaccagctg ccgggccttt agcgccgctt tcgatggtga aggacacttt ctgaccttcg
      601 tccagagatt tgtaaccatc gttctggata gcagagaagt gtacgaacac atctttagag
      661 ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt tgaaccattt tacgatacca
      721 gtcattttac cggacatagt gtattacctt taataattaa gtgtgccttt cggcgatatg
      781 gcgtgcttta cagattttga agcgttaaag gaatgtgcac tacgaggggt atcaacgata
      841 actcttgaag ggacttgcct tactacactg ctttaatggt ctgtacgtca aaccgttgat
      901 gtgcattaag ccacgcattg gcgggtgatg caacaattat ttttcatatt tatgattaat
      961 cggttggaaa acggtcctgt catcaggacc gtaaacagca ataaagtgga taaaagtcta
     1021 ttccatcaac tgcttactta atgccgggtt ggcttgaatc aaacgcatca attttagttc
     1081 ggcacttgaa ggcttcacgc gtctggattc ccattccttt accatggcga ctgatacgcc
     1141 caaaacccgg gcgaaatcat cgatttttaa ccctgtccct tttcgtaact gctcaatttc
     1201 ggtacaggac gttgttctgt gcgtcagggt aattttctgc gtttcatctt taaaaacaat
     1261 ctgttccagg ctgctcaaca gctcatgcat tggatcttta tattccattg aaaactcctc
     1321 aaatcacact gcgggatcgt gaattgcatc gaagctcatt aagaatagtc gggaaaagca
     1381 gacacagcgg ggtgaagtca gggcatgctc tggctatgat tcgattcagc ctgtcattta
     1441 gcgtgactta tagccaccta acgatctgat aaatgccgat atatctctta ccactcctgt
     1501 tttgtaaaga tttcgaaaat ctaaccttct gcgaaggcgt aaatctctgg ctacaaaaaa
     1561 ggcaacggca ctatcatttt ttaacaatga aacaacaaat taaccttgtt tcattttctc
     1621 catgatttta ccaggaagat tacgtagaaa gggtatcctg caagcctgtc ctggactatc
     1681 cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt ttgggtgaaa ggagtaagaa
     1741 aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct cttgcggcgt tattaatggt
     1801 agttagcctg agtgggtgtt ttgataaaga aggcgatcag cgtaaagcgt ttatcgactt
     1861 cctgcagaat acagtgatgc gtagcggtga acgtctacca accctgactg ccgatcagaa
     1921 aaaacagttt ggtccttttg tctctgatta cgcgattctg tatggttatt ctcagcaggt
     1981 gaatcaggcg atggattctg gtctgcgccc ggtcgtagac agcgttaacg caattcgcgt
     2041 accgcaggat tacgttacgc aaagcggtcc gctgcgtgaa atgaatggtt cgctgggtgt
     2101 gctggcgcaa caactgcaaa atgcgaagtt gcaggccgat gctgcgcact ctgcgctaaa
     2161 acagagcgat gacctgaaac cggtatttga tcaggcattc accaaagtgg taacgacgcc
     2221 agctgatgca ttgcaaccgt tgattccggc ggcacaaact ttcacgcaac aactggtgat
     2281 ggttggagac tacattgctc agcaaggtac tcaggtgagc tttgtcgcaa atggcatcca
     2341 gttcccgact tcacagcagg caagtgaata taacaaactg attgcgccat taccggcaca
     2401 acatcaggcg tttaatcagg cctggactac agcagttact gcaacccaat aaagagtaaa
     2461 agcccgagcg attatcgggc tttacttcac gcagtcgcgg cttagtccgc gacgtgagga
     2521 ttcacacagt tcttctcaac ctttccttgt aacgcatcaa ttaaattatc cacggcacag
     2581 gcggccatgc cataacgcgt ctcatgggtg gcagatccaa tatgcggtac tgcgacgacg
     2641 ttggccattg agagcaacgg cgaatctacg gacagtggct cttgttcgaa gacatcaagc
     2701 ccggcggcgt gaatttcccc tttctgtaat gctgcgatca gtgcattttc gtcaaccacc
     2761 gggccacgtc cggcattaat gaaaatggcg gaggatttca ttttggcgaa ttgttctgcg
     2821 ccaaacagat gatgcgtctc atcagttaac ggcaggatca ggcaaacgaa atctgactct
     2881 tgtaacagtg tatccaaatc gcagtagcgg gcgttgaagc gttcttctgc ttctttatgg
     2941 tggcggcgcg cgttatagag gatgggcatg ttgaagccaa agtgcgcacg ttgtgccagc
     3001 gccataccga tccgtcccat cccgacaatg cccagtgttt tatggtgaac gtcagtgccg
     3061 taccagtccg ggcctatgct cgcggtccat tcgcctgctt ttacccgttc tgctacctcc
     3121 acaacccgac gagcggtaga caacaccagc gccatcagcg tatcggcgac ggtttctgtt
     3181 aatacggttg gcgtgtgcat cagcagaatt tttcgggcgg taagcgcatc gacatcaaaa
     3241 ttgtcatagc cgacggagat cgttgatgtg gcatgcagtt tcggcatttt ttccagcaat
     3301 gcggcatcaa cattctcgtt tgaacccagt aaaccttcag cttcggcaaa aattgctgca
     3361 ttttgttcga cggtttgtgg gctgaggttt gccacctggt gaacggtgaa atgctcttgc
     3421 aggcgttgca gtaaatcatc aggtaaggct ttgtagagga taacggacgg cttcatgctt
     3481 ctctccattc actgataagg cgatcacctt agcctgaaat atgatgaagg aaaactgaag
     3541 aatgccaggt gatttctgca tcacctggca tgaaagggat tacagcgggc ttaaggtaat
     3601 ttctacacgg cggttttgcg ccttaccttc tgcggtgctg ttgctggcga ttgggttagc
     3661 cgggccaagg ccctgagtac ggatgcggct ggcgtccacg ccctgggtga tcaacgcgct
     3721 ggcaacggaa tccgcacgct gctgggagag acgcatgttc aggtcgtgac cccccgtgct
     3781 gtcggtataa ccaatcacgt taaccgccgt tttcggatac tctgtcagca ccattgccac
     3841 gccggtcagg gtgttagcgc ctgccggttt cagggtcgcg ctgctgctgt cgaaggtcac
     3901 attgttcggc atattgagga taatgttatc cccgctgcgg gttacgctaa caccagtgcc
     3961 gcgcattttg tcacgcagct tcgcttcctg cacatccatg taataaccca cgccgccgcc
     4021 cagagctgcg cctgctgctg cgccaatcag cgcgccttta ccgcgatctt tcttcgaaga
     4081 agagagcgca ccaatacccg cgcccacgag agagcccaga cctgcgccga tagcagattt
     4141 gcctgcttcg cgttcgccgg tgtaagggtt agttgtgcag ccagataccg ccagagcgcc
     4201 actcactacg gcggcaataa gataaacacg tttcttcatt gttaatcctt aataaccttt
     4261 ttattctttg ccacgggttc cgtggcggga gattatgccg cgtgaacatg a
//
LOCUS       DAZOXR010000263         4294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZOXR010000263 DAZOXR010000000
VERSION     DAZOXR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.270"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            146..253
                     /locus_tag="ACHUNN_004116"
     CDS             146..253
                     /locus_tag="ACHUNN_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0578331.1"
                     /translation="MTLAELGMAFWHDLAALVIAGILASMIVNWLNKRK"
     gene            629..736
                     /locus_tag="ACHUNN_004117"
     CDS             629..736
                     /locus_tag="ACHUNN_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0578332.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(823..2502)
                     /gene="bcsG"
                     /locus_tag="ACHUNN_004118"
     CDS             complement(823..2502)
                     /gene="bcsG"
                     /locus_tag="ACHUNN_004118"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0578333.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2499..2690)
                     /gene="bcsF"
                     /locus_tag="ACHUNN_004119"
     CDS             complement(2499..2690)
                     /gene="bcsF"
                     /locus_tag="ACHUNN_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0578334.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2687..4258)
                     /gene="bcsE"
                     /locus_tag="ACHUNN_004120"
     CDS             complement(2687..4258)
                     /gene="bcsE"
                     /locus_tag="ACHUNN_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0578335.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1097 a         1073 c         1119 g         1005 t
ORIGIN      
        1 tttatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca
       61 cctgacaaca tcacgggggc cattcttgaa cctgaacaac tcaagaatag ccgcgaaacg
      121 ttgtcattac aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca
      181 tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa
      241 caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga
      301 ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa cgattgcacg
      361 cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt ccggaacaga
      421 aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca atgttgcggg
      481 ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat
      541 gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa tagccgcgaa
      601 acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca tggccttctg
      661 gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg tgaactggct
      721 gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca atttgcgccc
      781 ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg ggtaaggcac
      841 ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa ctactgcatt
      901 tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt tccagtcaac
      961 attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg agatagctag
     1021 gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct tcatgccgaa
     1081 gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac gtaggccaga
     1141 tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga ccaccatcac
     1201 tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa agaatttctg
     1261 cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc cgtcatgcag
     1321 tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg taacgtccag
     1381 ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat
     1441 aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt cgcgaacttc
     1501 tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg taaagcccag
     1561 tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca gattagtgtg
     1621 cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt
     1681 ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat gcgacatcaa
     1741 cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca gtagttcaaa
     1801 tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg cctccgcgtt
     1861 atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag tttgtgcggg
     1921 catatcaccc actaccgatg cgccacccgt cgccgcaacg gttgccgctg cgttaccacc
     1981 cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac ttggtcccgc
     2041 cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa tgcgaatcca
     2101 ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca tctgccagtt
     2161 aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca cctgcgaacc
     2221 ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca aagcaaagcc
     2281 gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa tgggcatcag
     2341 cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc ccgcccacaa
     2401 caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc agtattgcca
     2461 gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt tgccctggct
     2521 ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc aaagaataaa
     2581 cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag cgggaaaaat
     2641 atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat gatgagcgct
     2701 ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg cgaccatcgt
     2761 gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg ccccattgtt
     2821 ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct tcaaaccaga
     2881 ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc aacgcggtat
     2941 ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg ccaatggtca
     3001 tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg cggatacccg
     3061 gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga gggttattca
     3121 tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc agtttgagcg
     3181 gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta aacttctgcc
     3241 cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac ggaataacca
     3301 tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta gcggtatttt
     3361 cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga atgctgcggg
     3421 ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc gcctgagcgg
     3481 tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt tccgatagcg
     3541 gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt
     3601 ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc tgaacgctaa
     3661 gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc agcaaatgtt
     3721 ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc tcaagcaaca
     3781 atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa caatggttta
     3841 acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca ggaatgtttt
     3901 gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca atagaacact
     3961 gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa aataatttta
     4021 ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg ctgtccatgc
     4081 taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc gccagactga
     4141 tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc atatgtcgca
     4201 gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg tccctcatgt
     4261 caccattttg tctgattatc agtttactag cgaa
//
LOCUS       DAZOXR010000264         4192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZOXR010000264 DAZOXR010000000
VERSION     DAZOXR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.271"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUNN_004121"
     CDS             complement(<1..215)
                     /locus_tag="ACHUNN_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0578336.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHUNN_004122"
     tRNA            complement(330..405)
                     /locus_tag="ACHUNN_004122"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHUNN_004123"
     tRNA            complement(412..486)
                     /locus_tag="ACHUNN_004123"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHUNN_004124"
     tRNA            complement(603..687)
                     /locus_tag="ACHUNN_004124"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHUNN_004125"
     tRNA            complement(696..771)
                     /locus_tag="ACHUNN_004125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUNN_004126"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUNN_004126"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0578337.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUNN_004127"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUNN_004127"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0578338.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUNN_004128"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUNN_004128"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0578339.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAZOXR010000265         4186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZOXR010000265 DAZOXR010000000
VERSION     DAZOXR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.272"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..324
                     /locus_tag="ACHUNN_004129"
     CDS             <1..324
                     /locus_tag="ACHUNN_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0578340.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="ACHUNN_004130"
     CDS             551..1816
                     /locus_tag="ACHUNN_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0578341.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="ACHUNN_004131"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="ACHUNN_004131"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0578342.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="ACHUNN_004132"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="ACHUNN_004132"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0578343.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4186
                     /locus_tag="ACHUNN_004133"
     CDS             3627..>4186
                     /locus_tag="ACHUNN_004133"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR0578344.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1092 a          938 c         1005 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcg
//
LOCUS       DAZOXR010000266         4151 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZOXR010000266 DAZOXR010000000
VERSION     DAZOXR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.273"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            145..1659
                     /gene="caiT"
                     /locus_tag="ACHUNN_004134"
                     /pseudo
     CDS             145..1659
                     /gene="caiT"
                     /locus_tag="ACHUNN_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
     gene            1690..2832
                     /gene="caiA"
                     /locus_tag="ACHUNN_004135"
     CDS             1690..2832
                     /gene="caiA"
                     /locus_tag="ACHUNN_004135"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0578345.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2961..4151
                     /gene="caiB"
                     /locus_tag="ACHUNN_004136"
                     /pseudo
     CDS             2961..4151
                     /gene="caiB"
                     /locus_tag="ACHUNN_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT          990 a         1052 c         1098 g         1011 t
ORIGIN      
        1 tcacagaata cagcttattg aatacccatt atgagttagc cattaacgcg tccacgaggt
       61 taataataat tatatcaaat gttaacaaaa ataaaacaaa cgggaacgca aaataaatat
      121 tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg aatagaaccg
      181 aaggttttct ttccgccgtt aataatcgtc ggcatacttt gttggcttac agtcagagat
      241 ctggatgcag cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa tgtatgggga
      301 tgggcatttg aatggtatat ggtggtgatg cttttcggtt ggttctggct ggtgtttggc
      361 ccgtatgcca aaaagcgttt aggtaacgaa ccacctgaat ttagtaccgc cagttggatc
      421 tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc gattgagatc
      481 tactactaca tctccacccc gccgtttggc ttagaaccga actcgacagg ggcgaaagag
      541 ttggggctgg cttacagctt gttccactgg ggacctctgc cgtgggccac ttacagcttc
      601 ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat tcgccccagc
      661 tcgacactgg tgccgctggt aggtgaaaaa cacgccaaag ggttgttcgg cactatcgtc
      721 gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg ccttgccacg
      781 ccgctggtga ccgagtgtat gcaatggttg tttggcattc cgcataccct gcaactggac
      841 gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcttg cggtctgcaa
      901 aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat gctgggttgg
      961 gtgttcattg tcagcggtgc cagcttcatc atgaactact tcaccgattc ggtggggatg
     1021 ttgctgatgt atctgccgcg catgttgttc tataccgatc ccatcgctaa aggcggcttc
     1081 ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttatgctat ccagatgagt
     1141 atcttcctcg cccgcatctc ccgtggtcgt actgtgcgtg aactgtgctt cggcatggtg
     1201 ctggggctga cagcgtcaac ctggatcctg tggactgtac tcggtagtaa cactctgctg
     1261 ttgatagata aaaacatcat caacattcca aatctgatcg aacagtacgg tgtggcgcgc
     1321 gccatcattg aaacctaggc cgctctgcca ctcagcaccg ccaccatgtg gggcttcttc
     1381 atcctctgct ttattgccac cgttacactg gttaacgcct gctcttatac cctggcgatg
     1441 tccacttgcc gcgaagtacg cgatggtgaa gaaccacctc tgctggtgcg tatcggttgg
     1501 tcaattctgg ttggcattat cggtattgtt ctgctggcgc tcggcggcct gaaaccgatt
     1561 caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacattat ggtgacgctc
     1621 tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa aatatcaaga
     1681 ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc cggtatccgc
     1741 gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg tgacagcgtc
     1801 tacccggaac gttttgtcaa agcactggcg gatatgggta tcgacagtct gctgatccct
     1861 gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg gatggagctg
     1921 ggacgtctgg gggcaccaac ctatgtgctg taccagttgc cgggcgggtt caacaccttc
     1981 ctgcgcgaag gcacacaaga gcagatcgac aaaattatgg ctttccgcgg caccggtaag
     2041 cagatgtgga actcagcgat taccgaaccg ggcgcgggct ccgacgtggg tagcctgaaa
     2101 acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg ttttattacc
     2161 agcagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc tccggacaaa
     2221 cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt gaccaaactt
     2281 gaaaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga cgtggaactg
     2341 gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa agaagagttc
     2401 gaccatgaac gtttcctggt agccctcacc aactacggta cggcgatgtg cgcttttgaa
     2461 gatgcggcgc gctacgccaa tcagcgcgtg cagtttggcg aggctattgg tcgtttccag
     2521 ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa aaacatgctg
     2581 tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc agcgatgtgc
     2641 aaatacttct gcgccaatgc ggcatttgaa gttgtggata gcgcaatgca ggtgctgggc
     2701 ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgatct gcgtgtagac
     2761 cgcgtctccg ggggatctga cgaaatgcag atcctgacgc tgggtcgcgc ggtgctgaag
     2821 caataccgct aagtccgtgc gctgcctgat gcgacgctaa tgcgtcttat caggcctacg
     2881 ggagcacgaa tgtaggccgg ataaagcgtt tacgccgcat ccggcagtca tgcactacat
     2941 aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcggg ccgttggccg
     3001 gattgcgcgt tgtcttctcc ggtatcgaaa tcgccgggcc gttcgccggg caaatgttcg
     3061 cagaatgggg tgcggaagtt atctggatcg agaacgtcgc ctaggccgac accattcgcg
     3121 ttcaaccgaa ctacccgcag ctctcccgcc gcaatttgca cgcgctgtcg ttaaatattt
     3181 tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat atcttcatcg
     3241 aagccagcaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta ctgtggcagc
     3301 acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc accgaggagt
     3361 acaccaatct tccggcctat aacaccatcg cccaggcctt cagtggttac ctgattcaga
     3421 acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac ttttctggcc
     3481 tgaccgccac cacggcggcg ctggcagcac tgcataaagt gcgtgaaacc ggtaaaggcg
     3541 aaagtatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac ttcatgatgg
     3601 attacttcaa cggcggcgaa atgtgcccgc gcatgagcaa aggtaaagat ccctactacg
     3661 ccggttgcgg tctgtataaa tgcgccgacg gctacatcgt gatggaactg gtgggcatta
     3721 cccaaattga agagtgcttt aaagatattg acctcgcaca tctactcggt acgccggaaa
     3781 tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca ctggtagaag
     3841 aaaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa cgctttgccg
     3901 aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc aatccacagt
     3961 atgttgcccg tgaatcaatc actcagtggc aaacgatgga tggtcgcacc tgcaaagggc
     4021 cgaacatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga atgccctcac
     4081 atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac gacattcagg
     4141 agttggtcag c
//
LOCUS       DAZOXR010000267         4131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZOXR010000267 DAZOXR010000000
VERSION     DAZOXR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.274"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            115..528
                     /locus_tag="ACHUNN_004137"
     CDS             115..528
                     /locus_tag="ACHUNN_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0578346.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="ACHUNN_004138"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="ACHUNN_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0578347.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="ACHUNN_004139"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="ACHUNN_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="ACHUNN_004140"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="ACHUNN_004140"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0578348.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="ACHUNN_004141"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="ACHUNN_004141"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0578349.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="ACHUNN_004142"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="ACHUNN_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0578350.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="ACHUNN_004143"
     tRNA            complement(3794..3881)
                     /locus_tag="ACHUNN_004143"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1101 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa a
//
LOCUS       DAZOXR010000268         4126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZOXR010000268 DAZOXR010000000
VERSION     DAZOXR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.275"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..1898
                     /gene="tssM"
                     /locus_tag="ACHUNN_004144"
                     /pseudo
     CDS             1..1898
                     /gene="tssM"
                     /locus_tag="ACHUNN_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3358
                     /locus_tag="ACHUNN_004145"
                     /pseudo
     CDS             1909..3358
                     /locus_tag="ACHUNN_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            3285..3767
                     /locus_tag="ACHUNN_004146"
     CDS             3285..3767
                     /locus_tag="ACHUNN_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0578351.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..4126
                     /locus_tag="ACHUNN_004147"
                     /pseudo
     CDS             3811..4126
                     /locus_tag="ACHUNN_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1129 a         1053 c         1083 g          861 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg caaaaaaaaa aaaaaaaaaa
     4021 aatactcagc ccaataacat catttgatta cattcatttt atcactacac atccttcagg
     4081 tataaaagat acactcgcct ggttagttaa cgcgagtaaa ttaatg
//
LOCUS       DAZOXR010000269         4108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZOXR010000269 DAZOXR010000000
VERSION     DAZOXR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.276"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..760)
                     /gene="nlpA"
                     /locus_tag="ACHUNN_004148"
     CDS             complement(<1..760)
                     /gene="nlpA"
                     /locus_tag="ACHUNN_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR0578352.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
     gene            982..1275
                     /gene="yicS"
                     /locus_tag="ACHUNN_004149"
     CDS             982..1275
                     /gene="yicS"
                     /locus_tag="ACHUNN_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR0578353.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(1316..2496)
                     /gene="nepI"
                     /locus_tag="ACHUNN_004150"
                     /pseudo
     CDS             complement(1316..2496)
                     /gene="nepI"
                     /locus_tag="ACHUNN_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2707..3159)
                     /locus_tag="ACHUNN_004151"
     CDS             complement(2707..3159)
                     /locus_tag="ACHUNN_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR0578354.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3212..>4108)
                     /gene="adeQ"
                     /locus_tag="ACHUNN_004152"
                     /gene_synonym="yicO"
     CDS             complement(3212..>4108)
                     /gene="adeQ"
                     /locus_tag="ACHUNN_004152"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR0578355.1"
                     /translation="LESLPGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGI
                     LGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSG
                     ALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAG
                     AFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALI
                     FVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRW
                     RDLNLCVVVVASLFALKIILVD"
BASE COUNT         1211 a         1080 c          912 g          905 t
ORIGIN      
        1 gtgattgata agattgcaga aattccttca cgttttctgc attcttatta tcttcccgtg
       61 ccaccaaaat attcacatac ggcgaattct tatcttcaat aaatacgctg tcgtgcaccg
      121 gagaaagccc tgtctgctga atgtaagtgg tgctgataat cgctacatca actttgggat
      181 catccagtac gcgcgggagc tgcgccccct cgagttccat aatctgcaaa tgacgaggat
      241 tatcggttat atccagcgcg gtaggtaata acccttttcc ctctttcaga gtaattagct
      301 tctctttttg caacagtaaa agtgcgcgac caaggttggt cgggtcgttg gggatcgcca
      361 ctgtcgcgcc ttcttttatt tgcgccacgg ttttgatttt tttggaataa cccgccatag
      421 ggaagacaaa cgtatttccc agcgctacca gtttataacc atgcgcctga ttatcttgtt
      481 caagaaaggg tcgatgctgg aagacattcg catcaagttc gccatgattt gtcgcatcgt
      541 tcggtagcaa cgaaccgcta aatcctacca gttcaacatc gaggccatat ttctcttttg
      601 ccaccttttt agcgacttcc gcgacatctt gttctgcgcc atttataacg ccaactttaa
      661 tgtgttttgc atcgctgcta ctctggtcgc aacctgccag cagaattccg gccagcaata
      721 atgcggcccc tgtccgtaga tgatgtgttg tcagtttcat ttttttatcc ttttattaat
      781 gattacctga ctatagcggt aaggtcgctg cggtttaaaa aacgaaacgc tatcgataag
      841 aataaaaagg aataaaagtg gaatataaag tctggcactt cttactgacc acgcaagcgc
      901 gtttcgtaca gcatgatgag agcgatgaaa gcaaactaca cttatgcttc attcgataca
      961 catttgtaaa aggttgaatc catgaaacca acgacactac tgcttatttt cacttttttc
     1021 gctatgccag gcatcgtata cgccgagtcg ccttttagct cactgcagtc cgcaaaagag
     1081 aaaaccacgg tattacaaga tttacgtaaa atttgcacgc cacaggcgtc attatcagat
     1141 gaagcgtggg aaaagttaat gttgtcagat gaaaacaata aacaacatat acgcgaggcc
     1201 atcgtggcga tggaacgtaa taatcagagc aattattggg aagcgttggg taaggtagaa
     1261 tgcccggata tgtaagaaca gcaacaacta ctgaccaacg cttctttacc tgatatcagg
     1321 atttcttcat tttcaccttt gcagtaacca acaatgcagt cagcaacatc aatgtgccag
     1381 aaaacatcaa cggcgaagtc agaccaatat tatccagcgc ataaccgccg attgccgcgc
     1441 cacaagctga ataaccgcca cctgaataga cccggctttt tctgcctgat cggccagtga
     1501 gcgggtgatc cacgttgacc agccgacggg aaccaatgca aaagttagcc cccagataat
     1561 cgccacgccg gtagcaacga ttttatcgct tccccacaac gtcagcacca acgcactcac
     1621 agccagtatt aacggcgcgc ctgctaaggc cagttttacc gaacgtttaa gaatgaatga
     1681 cgaaagcgac gtaccgataa agctggcaat gccaaaactc aacagcacca gcgttaagcc
     1741 atccacgctg aatcccgcca ggttcatata caccgggcga atatatgtga agaaagcaaa
     1801 ctgcccggcg aaagacatga agatggcgat catccctgcc atcacacccg gacgttgtaa
     1861 taagcggaaa gtattttgtt tctgatgcga gggttcgcct ggcagtgaag gcaatgattt
     1921 gataatccag aaaatacaca gcacgcccat caccgccgcc gcattaaaga cattgcgcca
     1981 accgataagc tcgcctaaaa aactgcccaa cggcgcggca atcaccagcg caatagaaac
     2041 cgcgccgaag atcaccgaca gcgccttcgg caccgtacgc ggcggcacca gacgcatggt
     2101 cagcgacgcc gacatcgccc agaacccgcc cagcgccagc cccagacagg cacgaccgat
     2161 taaaagcaaa ctgaatgagt tagcaaagga aaccagcaag caggagagcg tcagcaaaac
     2221 ggcaaacaaa ataacaacgt tgcggcggtc agtagcctga attgtctggg taataaacaa
     2281 actggcaaac attgccacaa aggcggtcac ggtcaccgat tgcccggcaa ccccttccga
     2341 aatgcctaaa tcctgggcca ttggcgtcaa caaactgacg ggcaaaaact caacgataat
     2401 cagacaggcg acacaaaacg ccaccgagaa aacggctgac cagttcggtc gggtgatggc
     2461 atccgcgccg cggttttcgg caataaattc actcatggtg ttacccgtgg catgtttcag
     2521 gggaaaagcc gaccacttta acattgaaat gtgtgacgca tttaacgttt ttgcaacttt
     2581 tacggtgttg cttgagctgg agttaagatc gaaacggaac agggcaaaga ttaccggatg
     2641 cggcgtgaac gtcttatcca cccgacaggt caggcgcggt ctgtaggctg ataagacgaa
     2701 gcggttttaa tgcagccagt gaacgccctc ggcaggctga aagaacgcgt tatagcgcat
     2761 ctgaaaagca ttaatatcct gcatttcagg ttcggtttcg cgcagaaaac ccagcgccag
     2821 cctgacctga gcatcggtaa tcggcgcgga aaggcgggct ttttgtaaaa tccgatgcca
     2881 ttgcagcagg ttctgttcgc taccatcgac aatgacaatt tgccagatga gcaacacctg
     2941 cgcggcgttt tgcaggtgcg actcatccgg gcgatgcaaa taacgtaaaa attcgtcacc
     3001 ggctgacttt cccatttgat cgaccatcga ctccacgggt acgacatcga tattcagccg
     3061 atcactgaga cggcgaatcg cttttctggc cccggatgtc agcacccgaa aaccaaccac
     3121 aaacactacc gccagcgtgg cgagcattat ccagatcatg agttctcctg cgttatcaga
     3181 actcatgata tctggaaacc ctcgccagtt actaatccac cagaataatc ttcagtgcaa
     3241 acagagatgc gaccaccacc acacacaggt tcagatcgcg ccagcgcccg gtgcacactt
     3301 tcatgatgca gtacgacata aagccgagtg caatcccttc ggtgatcgag aaagtaaagg
     3361 gcatcatcac cgtggtaata aacgcaggca ccgattcggt aaaatcatcc cagttaacgc
     3421 gcgccaggct cgaagtcatc agcacgccaa caaagattaa cgctccggcg gttgcgtaag
     3481 gaggaaccat cgccaccagc ggggagaaga acataaccaa caggaacata acgccaacca
     3541 caaccgcagt cagccccgtg cggccaccga ctgccacacc agaagtactt tcaatatagg
     3601 cagtaacaga cgaggtgccg ataaacgcac ccgccaccga actgacgcta tcaacataca
     3661 gcgccttatt catattgggg aatttaccgt taccatctat taagcccgct ttatcagtta
     3721 caccaattaa tgttcctgat gaatcaaata ggttgatcag cataaaggag aaaatgatac
     3781 cggcgagttc aagtgttaac gcgccgctca aatctacttc accaatgacg ccgctaatat
     3841 caggcggaat ggaatagacg ccgctaaaat gaacatcacc gaaaaataat ccacagcaag
     3901 acgtcaccac aatagaaacc agcaccgcgg catgaaaatg acgtgatgac aacacggtta
     3961 taataaaaaa acctaaaata cctaacaaca cgccgtgaga acttaaatcg ccaatcatca
     4021 ccagcgtgtc tttattggcg acaataacgc cagtattttt taatcccatt aaggcgataa
     4081 ataatccaat tccgggtaat gactccaa
//
LOCUS       DAZOXR010000270         4082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZOXR010000270 DAZOXR010000000
VERSION     DAZOXR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.277"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(46..747)
                     /locus_tag="ACHUNN_004153"
     CDS             complement(46..747)
                     /locus_tag="ACHUNN_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0578356.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPK
                     LQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1280..1534)
                     /gene="mobC"
                     /locus_tag="ACHUNN_004154"
     CDS             complement(1280..1534)
                     /gene="mobC"
                     /locus_tag="ACHUNN_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR0578357.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1713..3872
                     /gene="mobQ"
                     /locus_tag="ACHUNN_004155"
     CDS             1713..3872
                     /gene="mobQ"
                     /locus_tag="ACHUNN_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR0578358.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGL
                     AALRVQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNS
                     QLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRREEDARTHATERRHDRGWSQ"
BASE COUNT         1074 a         1032 c          986 g          990 t
ORIGIN      
        1 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt
       61 gatttgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
      121 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaatgc agggtcttaa
      181 taattgctgg ttaagtgatt tatatgtttg atatttatgc tctatatcaa gcattgagcg
      241 aaaatcatcg agagcgataa tacgttcgcc agtactgcgg aactgctgga ggagttcgta
      301 tatccttata ctgtaacttc ttgataatct agatatattc ttgattacaa ctctagtaaa
      361 ttgcccttgt agttgtgtca aataaggcat tactgcgtca gaaaatgtta tttgtgctct
      421 ggcctcgcct ttagcgtact gtgcccgata ctggatccag cggaactcct cacgtttctc
      481 gtcatccttg agaatgattg acctgtccca caaacgatca attgcttcct gtaactgaac
      541 ctcggcattt ttccgcccca tgtcagggaa tgaatcgaaa tattccgcag ctgtaatggt
      601 cattgtcttt tgcaattttg gagattctgc atcagcccca gacttcaatc gaccgatgca
      661 aagtaacaaa aatcgctgtt cctgtagggt caatttataa ctggcttcaa ccagcgcatt
      721 ggatttatag gcaattagat ccgacataga gcatccctcc ccaaaaggta atgaataaaa
      781 gataaacata ccctcaacga aaggcaatgc ctttttattt taatatccct ttttctgtga
      841 atattacctt aaatcatatt gcctttacct taaattcccc cacctaaaca ccttaaatca
      901 tattaccttt accttaaatc acattaccta tagcattgta acattttgtt ttctataagg
      961 aattttggcg ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     1021 acgacgagcc tgtggataac ttggagtgct gaaaacaaaa aaccaataaa aacaatgcgt
     1081 tgcaaaaaag cagaattgca agttccgaat acaccccctc caaccctgtg gaaatccagt
     1141 attcatgcgg gttcagcgca tatcacctcc cgaaaaaccg tgcgtatgtg ctcaatcagg
     1201 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctgggactg
     1261 cggcgagcgg acgatacaat cactgtcctg gcagcgcttc cagctcgtta atcagcggca
     1321 gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg agtaaccagg
     1381 cacgcaactg cacctcctcc ggctttctga cctttgccag caatgctgca cccacaatga
     1441 ccttctgcgc tgtgtccaga ctcctgctct tcgccttcag gcgctgtaat ctggcctcag
     1501 catcagcaat cttctgttcg agtgttctgc tcatgatgta actccgtgcg cgatgaaaaa
     1561 tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc acggaaaatc
     1621 acgaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc tgcggggctg
     1681 aaagaaaacc acaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa
     1741 tcatctccag aaacagcggc tacagcgccg ttgcttctgc tgcctatcgt tccggttcac
     1801 tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg
     1861 aagctgtgat tctgactccc gcgacagccc ctgcctggtg tacgaaccgt gccgaactct
     1921 ggaatgccgt tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac
     1981 tcgccatccc ccgcgaacta ccgcaggatg ccgcacggga aaccgttctc gcctttgtca
     2041 gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa
     2101 caaatcccca tgcgcacatc atgctgacca cgagagctgt cggacctgct ggattcggtg
     2161 gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgtgca tcatgggctg
     2221 accatgcgaa cagggcactg gcgaacgccg gttatcagga agagatagac catcgttcat
     2281 acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga
     2341 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacagactta
     2401 acctggaaat acagatttcc cgtaccgaac tcagaaacag cgggctgaca gagcctgccc
     2461 gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac
     2521 gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg
     2581 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
     2641 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
     2701 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa
     2761 aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg aaggtggaga
     2821 aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag tggggggcat
     2881 ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg cgccggaagg
     2941 agcaggagga tacagaacgt agtcgcgcgg agcgacgagc ggaacagctc aggaaaaatg
     3001 acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat ggcgaggccc
     3061 ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg aacggagaat
     3121 acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag cgtgttgatt
     3181 taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa catccaccac
     3241 tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt tgccagacat
     3301 ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct ttctgtcttg
     3361 gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat tttacgcagg
     3421 gacgtctgct accgacgctg atgccggtaa tgagttctgt cgaccgggaa acccgccagc
     3481 ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc cggcttaatg
     3541 gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg gtcatcagtg
     3601 cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat cacgatgcag
     3661 acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc cgtgaggcac
     3721 cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc cgctcgctgg
     3781 gttttaatcg tacctgggac gaatggcggc gcgaggaaga tgcacgaaca cacgccaccg
     3841 agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt tttgtttaac
     3901 atcgtgttat gttcgtcgat gttcttcgat gttcatcgac agggatgaat gcaagaagca
     3961 cacacataat gcaagcatag ttttgttttt ccgcccccaa aaaatggggg ttcttttttc
     4021 gctgcattag agcgcttttg acgcaatttt tcacttcaat agtaccttcg cactactaac
     4081 ac
//
LOCUS       DAZOXR010000271         4076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZOXR010000271 DAZOXR010000000
VERSION     DAZOXR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.278"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUNN_004156"
     CDS             <1..784
                     /locus_tag="ACHUNN_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0578359.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="ACHUNN_004157"
                     /pseudo
     CDS             797..1757
                     /locus_tag="ACHUNN_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="ACHUNN_004158"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="ACHUNN_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0578360.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="ACHUNN_004159"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="ACHUNN_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0578361.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAZOXR010000272         4049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZOXR010000272 DAZOXR010000000
VERSION     DAZOXR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.279"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHUNN_004160"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHUNN_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0578362.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUNN_004161"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUNN_004161"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0578363.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUNN_004162"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUNN_004162"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0578364.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUNN_004163"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUNN_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0578365.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1143 c          971 g          965 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccgat
//
LOCUS       DAZOXR010000273         3951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZOXR010000273 DAZOXR010000000
VERSION     DAZOXR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.280"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUNN_004164"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUNN_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR0578366.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUNN_004165"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUNN_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR0578367.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUNN_004166"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUNN_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR0578368.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHUNN_004167"
     CDS             complement(3668..3898)
                     /locus_tag="ACHUNN_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR0578369.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1008 c         1002 g          918 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag g
//
LOCUS       DAZOXR010000274         3935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZOXR010000274 DAZOXR010000000
VERSION     DAZOXR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.281"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..2602)
                     /gene="acrF"
                     /locus_tag="ACHUNN_004168"
                     /pseudo
     CDS             complement(1..2602)
                     /gene="acrF"
                     /locus_tag="ACHUNN_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="ACHUNN_004169"
     CDS             complement(2614..3771)
                     /gene="acrE"
                     /locus_tag="ACHUNN_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR0578370.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT          958 a         1038 c          974 g          965 t
ORIGIN      
        1 ttcctgatac gacatacctg tccagtcata accaatgccc gcaggtaatt ttgacgcaag
       61 gttttccatc aatgccatgg catcgccgga actggttcct ggcgcggctt ccccctgaat
      121 ctccattgac ggcagaccgt tgtagcgttc cagtcgcgga gagccataca cccaatgtga
      181 agtggtaaag gcagagaatg gcaccatttc gccgttggcg ctgcggacat aaagtttatc
      241 gacatcttct ggcagcatac ggaatttggc atccgcctga acatacacct ttttcacgcg
      301 accacggtcg atgaagtcgt taacgtaagt cccacccagc gccgttgaaa tggtctgatt
      361 gatgtcagaa agtgaaacac ctaatgcctg cgccttttcc tggtcaactt ccagtttaaa
      421 ctgcgcggtg tcttccagac cattaggacg cacgctgact aagctggcag gatgttgcgc
      481 cgccatgcca agcaactggt tacgggcctg ggtcagggca tcgtgaccca gcccagcctg
      541 atcaattaac tcaaagtcga aacccgttgc cgtgcccagt tcaacaatgg ctggcatatt
      601 gaatggaatg acaaaaccgt cgcggatctt gcccaattcc attttggcac gatggattac
      661 cgcttccgca ctgttttcgt caccactacg ctcttcccac ggtttcagac tgacgaaagc
      721 cataccggcg ttttgtgcct ggccgctgaa gctaaagccg ttaaccgtaa agacactttc
      781 tacgttcgct ttctcgttat tcagatagta atccgtcact tgatccaaca ctttttgcgt
      841 ccgctcttgc gtcgcgccag cgggtaactg aatcatggtc agaaagacac cctgatcctc
      901 ttcaggtaag aaggaagaag gaagacggaa gacgtaaaaa caacaccacc attcctgcaa
      961 caatcagcgc atagatcagt aaatatcgtc ctgtggaacc gaggattttg ccgacgctgt
     1021 tggtgtagtg gttaacgcta tgatcgaagg tggtattaaa ccagccgaag aaaccgccct
     1081 tattttcgtg atgctcagca gagacgggtt taagcagcgt tgcacataac gcaggggtaa
     1141 gaatcaatgc caccagaaca gaaagcgcca ttgccgaaac gatggtgata gagaactggc
     1201 gataaattgc cccagtagaa ccgccgaaga atgccatcgg aataaatacc gctgacagca
     1261 ccatcgcgat acccaccagt gcgccctgaa tttgcgacat cgatttttcc gtcgcttctt
     1321 ttggcgggag cttatcctcc atcatcacgc gctcgacgtt ctccaccacc actatcgcat
     1381 catcgacgag cagcccgatg gcaagcacca tcccgaacat cgttagtgtg ttgatggagt
     1441 aaccaaaagc ggcgaggatg gcaaacgtcc ctaacaacac cacgggtacc gcaatggtgg
     1501 ggatcagcgt tgctcgcata ttctgcaaga acagatacat caccaggaac accagcataa
     1561 tggcttcgaa cagcgttttt accacttcgt gaatagaaag ctggacgaat ggcgtggtgt
     1621 cataagggta gagaaccttc attccctgcg ggaagaatgg ctgtaattcc gccagttttg
     1681 ccttaatggc tttcgcggta ttgagagcgt tcgcgccggt tgctagctta atccccaggc
     1741 ccgccgccgg ttttccgttg atacgagcga taacgttata gttttcaccg ccaagttcaa
     1801 ctcgtgcgac atctttcagg cgtaccaccg agccgtcact gtttacgcgc agggttactt
     1861 tgccgaattc ttccggattt tttaaccgcg tctgagcaat aatcgaggcg ttcaactgtt
     1921 gccctggtaa cgctggcgtt ccgcccaact gcccggcagc gatctgatcg ttctgtacct
     1981 tcaactggtt aatcacatca accggtgtca gtttatattt gtttagcaga tcggcgtcca
     2041 gccagatacg catcgcatac tgtgcgccga aaagctgtac gtcaccgacg ccattcagac
     2101 ggctaagcgt atctttaacg ttagaggcca catagtccga gatatcgtcc tgtgtggtgt
     2161 ctgggttatc agagacaaag cccgccacca tcaaatagct gctactggac ttttcaacac
     2221 tgatcccctg ctgctgaacc tcctgcggca gcaacggcgt ggcgagctgg agtttgttct
     2281 gcacctgcac ttgcgcgata tcaggatcgg tcccggactg gaaggtaagg gtaattgtca
     2341 cgctaccggc ggaatcgctg gtggaggaca tatacatcag gttatcgata ccattcatat
     2401 tctgttcgat aacctgcgtc accgtatcct gcacggtctg cgcatcagca cctggatagt
     2461 ttgcctaaac agaaaccgca ggcggtgcaa tggttggata ctgagcgacg ggcaattgta
     2521 ggatcgccag tgcgcccgcc atcatcagaa taatggccag cacccatgca aatatcggtc
     2581 gtcgaataaa aaagtttgcc atgtcagatt accttacttc gatgcagtat ctgcgggggt
     2641 atcggtagtg gctttaacct gctctcccgg acgcgctttt tgcaggccgc tgacaatgac
     2701 ttgatcgcca gatttcagac cttcgctaat caaccattta tcgccaatcg cctgactggc
     2761 aacgacaggg cgcgcttcca cctgactttt atcgttaaca atcagcacgg ttgcatcacc
     2821 acgcggtgtg cggctaacgc cttgttgcgg gataagaatg gcgtcaggtt ggacgccttc
     2881 atcaatccgt gcacgcacaa acatacccgg caaaagcgta tgttgcgggt tagggaagac
     2941 agcacgtagg gttatggagc cggtgcattc atcaacggtc acatcagaga attgcagcgt
     3001 acctttcagg ggataggttt gaccgttttc catgaccaac tctacgttgc tggtggcgtt
     3061 ttccttatgc aaatttcctt gctctacgga ttgcttcagc ctcataaaat cgttgctgga
     3121 ttgggtcaca tcaacgtaga taggatcgag ctgctggaca gtcgccagtt cagtcgtttg
     3181 cccattagtg acaagagcgc cttcggtcac ggtcgatttg ccgatacgtc cgctaattgg
     3241 cgcagtcact ttggtataag cgagattgat gcgagcgctt tcgactgtgg cttttgcggc
     3301 aatcacggcg gcatcggcct ggcgagcatc agcaatggcc tggtcgtact cctgctggct
     3361 gatgtatttc gtacccacga gcggaacgta acgttttacc gtcaaatgcg cgatggcggc
     3421 ggcggcttca cttttcgcca gttcgccttt cgcgctgtca taatttgcct gataggtcgc
     3481 gggatcgatc tggtacaggg actggcctgc ttgtacatcg ctgccttcag tgaaattacg
     3541 attcagtacg atcccgctaa cctgtgggcg aacttcggct atacggtaag cattggtgcg
     3601 gcctggtaat tcagtcttaa cttctaacgg ggccgttttc acaatatgaa cggtaacctg
     3661 cggttcaccg acgtgagctt tctcttctcc cttatcgtta caaccgacga ttaaagccgc
     3721 ggagatcaga ataaaggagg gcaggaggaa aaacctggca tgtttcgtca ttactattcc
     3781 tcaaaaaaac aaaagcgcgt tatttaccca gaaaggcaac gcgttaactc gcagaaagaa
     3841 aaatacagtt tgctatccta caaattatca ttcgtcgatg taaggaatag ttatgaatac
     3901 tggcgtctca aggcacataa acacaaaaaa agatt
//
LOCUS       DAZOXR010000275         3917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZOXR010000275 DAZOXR010000000
VERSION     DAZOXR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.282"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            494..1525
                     /gene="repA"
                     /locus_tag="ACHUNN_004170"
     CDS             494..1525
                     /gene="repA"
                     /locus_tag="ACHUNN_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR0578371.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALKGQLGHAYNRLVKKDPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAEEISPKD
                     SEGHVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDREHRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKKR
                     REKAAASKRANRLKKLPVDQQIYEMAEHLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            complement(2594..3103)
                     /locus_tag="ACHUNN_004171"
     CDS             complement(2594..3103)
                     /locus_tag="ACHUNN_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578372.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIY
                     LSGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFFILDRQSLLNLMEATKDSPDVKQEL
                     LNRLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIRNFIDEQQLTNSDIPKKSTV
                     PNEDKKGIR"
     gene            complement(3159..3761)
                     /locus_tag="ACHUNN_004172"
     CDS             complement(3159..3761)
                     /locus_tag="ACHUNN_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR0578373.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKKRRFPRPSHWTREYTHECVEKIKILFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKLLRMQAQQENISGQ"
BASE COUNT         1033 a          965 c          937 g          982 t
ORIGIN      
        1 gtgagtagga caaaagtcga tcttttaatc caaaagaaag atctaaagca gaaaaatctg
       61 cttttaaaaa tagattttat ttttttgacg caggtcaaga ttaacttatt agaggtatca
      121 gtgaggaggc actggaagag aagagatcgt tgtaatgctt ttcaaattaa cgtaaagcgg
      181 gtatatttcg gctgttatta gctgtgcaga gggtggcact ctgtggagca aagcggcgaa
      241 agccggacgg cagaatgcgc cataaggcat tcaggagaga tggcatgtac gggcagtaag
      301 tcagaagact gaagatgttc cggaagccat aaaaggaaaa cccccactat ctttcttacg
      361 aacttggcgg aacgacgaaa gatagtgggg gcctcacaga atacgggtaa agtataatga
      421 aaccgtacca gagattcaac cctgtgcagt gtataaatac acggcacaat cgctccgcca
      481 taagcgacag cttgtggcag gtctgaagaa tactccatat aacgcagtac actggagtca
      541 gttagcaccc gaagagcaga tccgtttctg ggaagactat gaagcgggaa gggcgaccac
      601 tttcctggtt gaaccggaaa ggaagcgcac gaagcgccgt cgcggtgagc actccaccaa
      661 acccaaatgc gaaaatccgt cctggtatcg tcctgagcgc tataaagcgc tgaaagggca
      721 gcttgggcac gcctataacc gtctggtgaa aaaggacccg gtgaccggcg agcagagcct
      781 gcgcatgcgc atgtcgcggc atccttttta tgtacagaaa cggacgttcg tcggtcgcaa
      841 atacgctttc cggccggaaa aacaacgtct cctcgatgcc atctggccgg ttctggtcag
      901 cttcagtgat gccggcacac ataccgtggg gatgagcgtc actcggctgg ctgaagaaat
      961 cagcccgaaa gacagcgagg gacatgttat tccggaactg gaagtgacgg tctcccgtct
     1021 ttcccgtctg ctggccgaac aggtgcgctt tggtgtgctg ggtgtgtcag aggaaacaat
     1081 gtgggaccgt gaacatcgtc agcgtctgcc acgttacgtc tggataacac cggcaggctg
     1141 gcagatgctg ggcgtcgaca tggtaaaact tcacgaacag cagcagaaac gactgcgtga
     1201 aagtgaaatc cgccagcagc tcattcggga aggtgttctg cgtgaggatg aagatatctc
     1261 cgttcatgcg gccagaaaac gctggtatct gcagcgcagc caggatgccc tgaagaagcg
     1321 ccgagaaaaa gccgcagcca gcaagcgtgc caaccgcctg aagaaattac ctgttgacca
     1381 gcagatttat gagatggctg agcatctccg gaagcgtctg ccgccggatg aagcctattt
     1441 ctgttccgat gaccatctga agcgactggc catcagggag ttgcgtcagc ttgaactgac
     1501 gctggctgcc ccgccaccgc actagacagc atcattccct cagcactgaa tcatcgccag
     1561 cccctccggg gctttcggcg cagattccgc tcagcccaaa atccttagta gtcaccttaa
     1621 atcccctcag aggggcatat ctgcccataa aaccacgcat cagtcatcag aacatggcca
     1681 cgttgtttca gttatccaca taaatccgca aacaaagaac tttaagaagc tgcaaacctg
     1741 aaacagcaaa cctgcaatat agtcttaacc ccattattta atcccctgcg ttgcttcgcc
     1801 gcagggaaaa tctttatctc tgagaccact gtgaacaaat acaaagaggc cttcgcttgc
     1861 agcggccaag gccgcgccgc tcagaatcta aaagcacctc ccacgctgac gcgcgggccc
     1921 cgaacctcac cgttctgaaa ccacaacaaa aaaacatcag gaataaaaac accacacaaa
     1981 cgcagcaccg tgcccacccc tcataactga aaagcgaggc cgcccccgcc caaagggcgg
     2041 gaacaacatc gcttttaatt atgaatgttg taactacatt gtcatcgctg ccagtcttct
     2101 ggctggaagt cctcagtaca cgctcgtaag cggcccttac ggcccgctaa cgcggagata
     2161 cgccccgact gcgggtaaac ccttgtcggg accactccga ccgcgcacag aagctctgtc
     2221 atgactgaaa acgggtatgg tctagcaggg ctggggatgg gtaaggtgaa atctatcaat
     2281 ccgtaccggc ttacgccggg cttcggcggt tttactccgg tatcacatgt aaccacagcg
     2341 tgccgccttc catgccgctg acgcggcata tcctggtgac gatatctgaa tcgttatata
     2401 ctgtgtataa tatatggtgc gttaattttt aaacccttat ttaatttcct cgtcagttag
     2461 atattgtccg tttcccaaac gaagcccttc agagacgtcg tgtaaacacg gtccttttat
     2521 cttacaggtt aacccctatc aatcttagga tgaaaccacg ccatcctctg cttgcctggt
     2581 tattaatttc atatcatctt atgccttttt tatcttcatt tggaaccgta cttttcttcg
     2641 gaatatcact attcgttaac tgctgttcat caataaaatt cctgatgata ttcagttcac
     2701 tctgacggat tttttcttct tcattttttc tcagaagacg gcagatatcc atttcatccc
     2761 gcccggtcat tctttttccg gaaaaaaggc ggttaagcag ctcctgcttt acatctggtg
     2821 agtctttggt ggcctccatg aggttgagca gactctgacg atcgagaata aagaaaactt
     2881 ttgggaaata atgtaaaagg aaaagaaccc agaatgctgt ccatgcaaag cagagaaaag
     2941 ctgctgtaag cacgtgattc ttgcctgata ggtatataat cagcagaaca accgaaatcc
     3001 aagatgccag ttctaaaaaa gtatatacag gcttcaggcg tctgtatgcc tgttcatgat
     3061 tattcaggat gaattgaatt ttttctgcac ctgatttact cataataatt ctcttcccat
     3121 ttttctgata cgtaatgtcc agctgttatc aggagtgttt attgcccaga aatattctcc
     3181 tgctgtgcct gcatcctgag caacttttgt tctaatgtcg ccagtctgcg accggcactc
     3241 tgtgcttcag agtctgagac ctgccctttg gggtgaccat ccagaccata acgtgggacg
     3301 ccggcgacag ctgtccgctg cagatataca cggcgtgagg tgatgcagga cactgcacag
     3361 agccattcgt ccatggtcag ttctgtctcc gggtgttctg ccagaaatgc tgaaatatca
     3421 ttgtttatgc caattttcag gggaataaat ccaccacctt cagcacgaag atgaggaaac
     3481 aggatcttta ttttttccac gcactcgtga gtatactcac gggtccagtg ggagggacgt
     3541 gggaaacggc gttttttctt tttcttcttc ctggctggcg cttcggggat atgtgtttcg
     3601 acggcagatg aatttattcc ggcgggggct gattctgccg tctgttcagt aaatacaggt
     3661 gcgataatat ctgttttttc ggataaggac ggtggcgaaa aagtacgacg ttttttcacc
     3721 acaatgacgg gagttttcct ttcttcctga ttttttccca taaatacctc ggctggctgg
     3781 ttttactctt taataatatc acaatccata aattagtaaa tttcccatca ggtgattttt
     3841 catggctaat aagtccattc tctgaatatt tatactcgag gtagttacag ttatggcgtg
     3901 acagtcgaat tgatatt
//
LOCUS       DAZOXR010000276         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZOXR010000276 DAZOXR010000000
VERSION     DAZOXR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.283"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHUNN_004173"
     CDS             24..590
                     /locus_tag="ACHUNN_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0578374.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHUNN_004174"
     CDS             594..1169
                     /locus_tag="ACHUNN_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0578375.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHUNN_004175"
     CDS             complement(1211..2263)
                     /locus_tag="ACHUNN_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0578376.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUNN_004176"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUNN_004176"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0578377.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHUNN_004177"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHUNN_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          951 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZOXR010000277         3885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZOXR010000277 DAZOXR010000000
VERSION     DAZOXR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.284"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..246)
                     /gene="yneK"
                     /locus_tag="ACHUNN_004178"
     CDS             complement(<1..246)
                     /gene="yneK"
                     /locus_tag="ACHUNN_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0578378.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYN"
     gene            complement(323..1204)
                     /locus_tag="ACHUNN_004179"
     CDS             complement(323..1204)
                     /locus_tag="ACHUNN_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0578379.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            1305..2693
                     /gene="sad"
                     /locus_tag="ACHUNN_004180"
     CDS             1305..2693
                     /gene="sad"
                     /locus_tag="ACHUNN_004180"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR0578380.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2757..3683
                     /gene="glsB"
                     /locus_tag="ACHUNN_004181"
     CDS             2757..3683
                     /gene="glsB"
                     /locus_tag="ACHUNN_004181"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0578381.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3683..>3885
                     /locus_tag="ACHUNN_004182"
     CDS             3683..>3885
                     /locus_tag="ACHUNN_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0578382.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
BASE COUNT          952 a          902 c         1056 g          975 t
ORIGIN      
        1 attgtatagc gtgtcgttac cccggaaaga agtcctctga atatatgata agttatcaag
       61 gttattaaat atcttttttg tggctgagtt gatgtttaaa gacgtgcgcc actcttcatc
      121 acatattatt gtacttagcg catcaataaa gtcctttaaa acttctttta cctgtggtgc
      181 aatattacga acgggaaaat catcaggaag agatatgtaa ttgctgatgc ttactggcgt
      241 aaccatattt gccccatata gtcataagtc atcggaacat cactatatag agcatattag
      301 ccgcaagaat tatcaaaata gttcattggt aactctgttt tgccgagtca gacacatcca
      361 gtagttgaat aaatgcctca agcggacgcg ttttcgcacc gcgacgccag accagccagg
      421 ttgttaacca acgccattgc tcagctaacg gccacgcttc aacctggtga tgccccggca
      481 tactttccag catagagcgt ggaataagcg caatgcctgc tcctgcgacc acacaggcca
      541 acatcccgtg atacgactcc atctcgtgaa tagttcccgg agcggcaccg tcagcatgaa
      601 accagctctc gaagtggcga cgatacgaac aattggtgcg gaaggcataa atgttactgc
      661 cgttaacctg actggcgcgg attactggcg catgaccttg tggcgtgacg atcatcagct
      721 cctcgcggta taccggtatc ccgtcaatgg cagtatggtt aatgggccca tcaataaatg
      781 ccgcattcag ttttccctcc agcactcctt ccagcatagt gccggaaggg ccggtggaaa
      841 gtgaaaactg aattttggga taacgatggt tgaattccgc cagcgtggcc ggaatgcgca
      901 ctgctgcggt gctttccagc gaaccaagag aaaataaacc ttgcggctca tcgccagcga
      961 caacgctccg cgcttcatcc actaacgcga gaatttgttg gctatagcgt aaaaagttat
     1021 gcccggcagg agagagacgt aaacgctgat tctcgcgaat aaacagatct acccccagtt
     1081 ctgtttccag ctggcgtaga cgggtagtga gattcgacgg tacgcgatgc acttttgctg
     1141 cagcctgggt aatgctgcca gcttcggcaa ccgcgttgaa catctccagt tgggtcagat
     1201 ccataacatt ctcgattcgt gaataagtgg cttaatatta ttcattttaa agcaagagta
     1261 aatctgcgta tcttcatacc atgactcata aaggagatac cccgatgacc attactccgg
     1321 caactcatgc aatttcaata aatcctgcca cgggtgaaca actttctgtg ctgccgtggg
     1381 ctggcgctaa cgatatcgaa aacgcacttg agctggcggc agcaggcttt cgcgactggc
     1441 gcgagacaaa tatagattat cgtgctgaaa aaatgcgtga tatcggtaag gctctgcgcg
     1501 cccgtagcga agaaatggcg caaatgatca cccgcgaaat gggcaaacca atcaaccagg
     1561 cgcgcgctga agtggcgaaa tcggcgaatt tgtgtgactg gtatgcagaa catggtccgg
     1621 caatgctgaa ggcggaacct acactggtgg aaaatcagca ggcggttatt gagtatcgac
     1681 cgttggggac gattctggcg attatgccgt ggaattttcc gttatggcag gtgatgcgtg
     1741 gcgctgttcc catcattctt gcaggtaacg gctacttact taaacatgcg ccgaatgtga
     1801 tgggatgtgc tcagctcatt gcccaggtgt ttaaagatgc gggaatcccg caaggcgtat
     1861 atggctggct gaatgccgac aacgacggtg tcagtcagat gattaaagac tcgcgcattg
     1921 ctgctgtcac ggtgaccgga agtgttcgtg ctggagcggc tattggcgca caggctggag
     1981 cggcactgaa aaaatgcgta ctggaactgg gcggttcgga tccgtttatt gtgcttaacg
     2041 atgccgatct ggaactggcg gttaaagcgg cggtagccgg acgttatcag aataccggac
     2101 aggtatgtgc agcggcaaaa cgctttatta tcgaagaggg aattgcttct gcatttaccg
     2161 aacgttttgt ggcagctgcg gcagccttga aaatgggcga tccccgtgac gaagagaaca
     2221 ctctcggacc aatggctcgt tttgatttac gtgatgagct gcatcatcag gtagagaaaa
     2281 ccctggcgca gggtgcgcgt ttgttactgg gcggggaaaa gatggctggg gcaggtaact
     2341 actatccgcc aacggttctg gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa
     2401 tgtttggccc cgttgcggca atcaccgttg cgaaagatgc agaacatgcg ctggaactgg
     2461 ctaatgatag tgagttcggc ctttcagcga ccatttttac caccgacgaa acacaggcca
     2521 gacagatggc ggcacgtctg gaatgcggtg gggtgtttat caatggttat tgtgccagcg
     2581 acgcgcgagt ggcctttggt ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt
     2641 tcggcttaca cgaattctgt aatatccaga cggtgtggaa agaccggatc tgacgcgaca
     2701 tattcagctc tgatatactc gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg
     2761 cagtcgccat ggataatgca attttagaaa acatcttgcg gcaagtgcgg ccgctcattg
     2821 gtcagggtaa agtcgcggat tatattccgg cgctggctac agtagacggt tcccgattgg
     2881 gcattgctat ctgtaccgtt gacggacagc tttttcaggc cggagacgcg caagaacgtt
     2941 tttccattca gtctatttcc aaagtgctga gtctcgttgt cgccatgcgt cattactccg
     3001 aagaggaaat ctggcaacgc gtcggcaaag atccgtctgg atcaccgttc aattctttag
     3061 tgcaactgga aatggaacag ggtattccgc gtaatccgtt tattaatgct ggtgcgctgg
     3121 tggtctgcga tatgttgcaa gggcgattaa gcgcaccacg gcaacgtatg ctggaagtcg
     3181 tgcgcggctt aagcggtgtg tctgatattt catacgatac ggtggtagcg cgttccgaat
     3241 ttgaacattc cgcgcgaaat gcggctatcg cctggctgat gaagtcgttt ggcaatttcc
     3301 atcatgacgt gacaaccgtt ctgcaaaact actttcatta ctgcgctctg aaaatgagct
     3361 gtgtagagct ggctcggacg tttgtctttc tggctaatca ggggaaagct attcatattg
     3421 atgaacctgt agtgacgcca atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg
     3481 gtatgtacca gaacgcgggg gagtttgcct ggcgggtggg actaccggcg aaatctggcg
     3541 ttggtggcgg tattgtggcg attgttccgc atgaaatggc catcgcagtc tggagtccgg
     3601 aactggatga tgcaggtaac tcgcttgcgg gtatcgccgt ccttgaacaa ttgacgaaac
     3661 agttaggacg ttcggtttat taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg
     3721 ttcaaaattt cgctctcgct ttcgtctggg catgaaagag cgtcagtatt gcctggagaa
     3781 aggcgcgcca gtcatcgaac aacatgcggc ggattttgtc gctaaacgtc ttgctccggc
     3841 aggtaatgac tccaacttat tgatagtgtt ttatgttcag ataat
//
LOCUS       DAZOXR010000278         3864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZOXR010000278 DAZOXR010000000
VERSION     DAZOXR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.285"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHUNN_004183"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHUNN_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUNN_004184"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUNN_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0578383.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHUNN_004185"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHUNN_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR0578384.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHUNN_004186"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHUNN_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR0578385.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          845 c          959 g         1072 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc ttgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAZOXR010000279         3858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZOXR010000279 DAZOXR010000000
VERSION     DAZOXR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.286"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="ACHUNN_004187"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="ACHUNN_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0578386.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUNN_004188"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUNN_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0578387.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUNN_004189"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUNN_004189"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0578388.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUNN_004190"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUNN_004190"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0578389.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAZOXR010000280         3855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZOXR010000280 DAZOXR010000000
VERSION     DAZOXR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.287"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..302)
                     /gene="chbB"
                     /locus_tag="ACHUNN_004191"
     CDS             complement(<1..302)
                     /gene="chbB"
                     /locus_tag="ACHUNN_004191"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0578390.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAI"
     gene            complement(601..939)
                     /gene="osmE"
                     /locus_tag="ACHUNN_004192"
     CDS             complement(601..939)
                     /gene="osmE"
                     /locus_tag="ACHUNN_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0578391.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1141..1968
                     /gene="nadE"
                     /locus_tag="ACHUNN_004193"
     CDS             1141..1968
                     /gene="nadE"
                     /locus_tag="ACHUNN_004193"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0578392.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2198..3085
                     /gene="cho"
                     /locus_tag="ACHUNN_004194"
     CDS             2198..3085
                     /gene="cho"
                     /locus_tag="ACHUNN_004194"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0578393.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3045..3620)
                     /gene="ves"
                     /locus_tag="ACHUNN_004195"
     CDS             complement(3045..3620)
                     /gene="ves"
                     /locus_tag="ACHUNN_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0578394.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT          984 a          958 c          920 g          993 t
ORIGIN      
        1 ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc
       61 gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa
      121 gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt
      181 tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt
      241 gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc
      301 ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc
      361 ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat
      421 acggtataga cctgagcaat agtatttctt tgaccttata aattgtttct ccttgacctg
      481 tggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta agaactcagg
      541 ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca gacgaattga
      601 ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat
      661 gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc
      721 caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag acgaaggttt
      781 acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg
      841 ctgtacaaac tggtctttag tacgatcata agccgtacaa cccgccagca tggttaatac
      901 cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc ttgtttatca
      961 gcgtgttaga taagcctgga atacattggg cgctttttca agcccgcgaa cgaaacggct
     1021 ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc
     1081 gtttcagtgg caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca
     1141 atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa
     1201 gaggaaattc gtcgtagtat cgattttctg aaaagctacc tgcaaactta tccgttcatt
     1261 aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg aaagctgtgc
     1321 cagatggcga ttaatgagct gcgcctggaa accggcaacg aatcactgca atttattgcc
     1381 gtacgcctgc cctatggtgt tcaggccgac gaacaagatt gccaggatgc cattgccttt
     1441 attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc cagcgaacag
     1501 gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt
     1561 gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc
     1621 gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg cggtacagac
     1681 attaatccgc tgtatcgtct caacaaacgt cagggtaaac agttactggc ggcattaggt
     1741 tgcccggaac acctttataa gaaagcgcca acggccgatc tggaagatga tcgcccttct
     1801 ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg
     1861 aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat
     1921 aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata atttgcacat
     1981 aatggtttgt gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa cgccttattc
     2041 gacctacaaa actatgcaaa ttcaatatat tgcaggagtc gaataggcct gacaggcgta
     2101 gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct tttttaatcg ctccccgcct
     2161 gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc
     2221 gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct
     2281 taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc
     2341 gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt tacgcacccc
     2401 ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgta ccgcaggcga
     2461 aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa
     2521 acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt
     2581 ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc tgtttgccaa
     2641 taggcgcgca gctttgcaag cattgcagac catcgctgat gaacaaaaac tttgttatgg
     2701 cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg
     2761 ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac gcttgcgcca
     2821 gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga
     2881 acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt
     2941 taattcgctg gaagaagcga caacgttaat tcggacaccc gccgggtttg atcacgacgg
     3001 ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc
     3061 ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa
     3121 agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcaattta
     3181 tcgcccagtt gccacgcccc gttaatcaca aacaccaccc cgccgcgtga gccaaaggta
     3241 gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg tgtcatgatg
     3301 ttgaaatcca tcgacatctg cccttccgtc agtttcgctt ttaccacctg gtccgctgca
     3361 aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc ggtctgcgct ttcaaggagc
     3421 atctcgccgc cttccagcaa cgtcactatc ctttccatgc cgggaaacaa agaaaactcc
     3481 ccattcgccg cgatggatgc aatgctggca cgccagtaaa aatcacgttt tgccggtggg
     3541 aaagtgcaaa tttcgcgcgt ttcaccggca gcatttcgcc acagattcac cgacatttta
     3601 cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt atcgttcagg
     3661 taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggtgatc aggctttttt
     3721 tacaactgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg
     3781 tagtggacaa gaccggcggt cttaagtttt ttggctgaaa gattattcag cagttgcagg
     3841 cattttacct tttgc
//
LOCUS       DAZOXR010000281         3836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZOXR010000281 DAZOXR010000000
VERSION     DAZOXR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.288"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            79..1425
                     /gene="yagA"
                     /locus_tag="ACHUNN_004196"
     CDS             79..1425
                     /gene="yagA"
                     /locus_tag="ACHUNN_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR0578395.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNEHPPFKKHMYGVFMHLRY"
     gene            1704..3584
                     /locus_tag="ACHUNN_004197"
     CDS             1704..3584
                     /locus_tag="ACHUNN_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFR0578396.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKI
                     NAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRP
                     LFVKTETIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFW
                     GI"
     gene            complement(3606..>3836)
                     /locus_tag="ACHUNN_004198"
     CDS             complement(3606..>3836)
                     /locus_tag="ACHUNN_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFR0578397.1"
                     /translation="WCISLCGMVLYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMG
                     LLAVYTGTVFILSLPLSMIYILSVIIKRLSVR"
BASE COUNT         1140 a          822 c          940 g          934 t
ORIGIN      
        1 tctctgcctt tgtttcatgg ggggttcttt gtatatttta cttactgttt aagcatgtac
       61 ataaggagtg ctttcattat gccaatgata cttgtaaaga agaatccccg gggcaaagtg
      121 attcgggagc ttagcagtga ggaagagact gcagtaaaga cggtctgtgg actgaaaagg
      181 ccagcaacca tggcacaaca taacctggcc aatgatcttc tccgggaaat gcgtgagtat
      241 gatgcatggt tacagtgtga ctgtattccg ggagattcgc cggccatgaa ttttgctgct
      301 cttaaaaaca atacgggtac tctttatctg tccagtttta atcatgagca tgcacctgaa
      361 tgccccatgt atcgtcagtt atccggaaat gaagaggaaa catccttcgg agcaagccgg
      421 catcccgtta gcacccgtat aaattacaga aactttttac cacctgatga cagtaattct
      481 gttatcagac tgcaggcccg ctctgcttac cataatggtg agcgctcctc cgttcgcaag
      541 aaacgtccgc gccttggccg tttactcctg tcgcttattg aagatgcagg gctgaataaa
      601 ctggattccc tggctaatcc ccgtatccgg actaattatc gtgagtgtct tgatgccata
      661 cggcaggtta cactacagca ggagtatatt cggggaagag ccttatctga aattatccat
      721 ttccggccag gaatgtctga aaggtctcag gagcggttaa tggaaacgct ggagaattca
      781 gaacgacact ggcctgccag aagaaagcat atgttctttc agatttttat ggcgcagcac
      841 atctgtcgtg atgctgtgga aattcactgg gcgaacggta atattcaggt catcagacct
      901 gtgcggggga tcagtattaa tggtgaagcg cagggcggaa tacgtccccc ttactgggtt
      961 attcttgctt tttgccggag tgccgatggc agaatcatct gcagtgaagg gtatgctcat
     1021 gctctttatc aactgacatg tccggtgcct gtggacagca aactggaacg aaacacgctc
     1081 actgctcttc tgaatgtggc cagctggctt aaaagaaagc caggtacgcc ggaattaagt
     1141 ctggaaaggc ccctgtttga tacagaagtt tatgttaatg gtgaaaagaa atatgtactg
     1201 ccggatttca ttgtcacagc aagggctcct gacggaaaga cggccagagt ggtcatcgaa
     1261 acgatgggat atgaagacag tgattactgc gcgagaaaat ccaggcagca taccggcatg
     1321 aagcagattg gtgttctgca taccgatcca ccgaaatggc tggataacga gcatccccct
     1381 tttaagaaac atatgtacgg tgtttttatg catctcaggt actgagatat tttgtggctc
     1441 agttctgtaa cttttcccgt aacattgtct gttgttacgg gaaagtccgg tttttgtatt
     1501 gcaccagaga atacccagac tgtgatgctg ccacagcgtc agcaggcttt ctgaacggtg
     1561 taacatcact tcatgttaat gataatcact atcatcaaat cttgacatgc cattttctcc
     1621 ttaataaatt aatactgtat atgtatccat atgcgtaagc agttaattca tttgttttcc
     1681 tcagaggatg aaggagatac cgaatgtctg accctgtacg tattacaaat cccggtgcag
     1741 aatcgctggg gtatgattca gatggccatg aaattatggc cgttgatatt tatgtaaacc
     1801 ctccacgtgt cgatgtcttt catggtaccc cgcctgcatg gagttccttc gggaacaaaa
     1861 ccatctgggg cggaaacgag tgggttgatg attccccaac ccgaagtgat atcgaaaaaa
     1921 gggacaagga aatcacagcg tacaaaaaca cgctcagcgc gcagcagaaa gagaatgaga
     1981 ataagcgtac tgaagccgga aaacgcctct ctgcggcgat tgctgcaagg gaaaaagatg
     2041 aaaacacact gaaaacactc cgtgccggaa acgcagatgc cgctgatatt acacgacagg
     2101 agttcagact cctgcaggca gagctgagag aatacggatt ccgtactgaa atcgccggat
     2161 atgatgccct ccggctgcat acagagagcc ggatgctctt tgctgatgct gattctcttc
     2221 gtatatctcc ccgggaggcc aggtcgttaa tcgaacaggc tgaaaaacgg cagaaggatg
     2281 cgcagaacgc agacaagaag gccgctgata tgcttgctga atacgagcgc agaaaaggta
     2341 ttctggacac gcggttgtca gagctggaaa aaaatggcgg ggcagccctt gccgttcttg
     2401 atgcacaaca ggcccgtctg ctcgggcagc agacacggaa tgacagggcc atttcagagg
     2461 cccggaataa actcagttca gtgacggaat cgcttaacac ggcccgtaat gcattaacca
     2521 gagctgaaca acagctgacg caacagaaaa acacgcctga cggcaaaacg atagtttccc
     2581 ctgaaaaatt cccggggcgt tcatcaacaa atcattctat tgttgtgagc ggtgatccga
     2641 gatttgccgg tacgataaaa atcacaacca gcgcagtcat cgataaccgt gcaaacctga
     2701 attatcttct gacccattcc ggtctggact ataaacgcaa tattctgaat gaccggaatc
     2761 cggtggtgac agaggatgtg gaaggtgaca agaaaattta taatgctgaa gttgctgaat
     2821 gggataagtt acggcaacga ttgcttgatg ccagaaataa aatcacctct gctgaatctg
     2881 cggtaaattc ggcgagaaat aacctcagtg ccagaacaaa tgagcaaaag catgcaaatg
     2941 acgctcttaa tgccctgttg aaggaaaaag agaatatccg taaccagctt gccggcatca
     3001 atcagaagat agcggaagag aaaagaaaac aggatgaact gaaggcaacg aaagacgcaa
     3061 ttaatttcac aacagagttc ctgaaatcag tttcagaaaa atatggtgca aaagctgagc
     3121 agttagccag agagatggcc gggcaggcta aagggaagaa aatacgtaat gttgaagagg
     3181 cattaaaaac gtatgaaaag taccgggctg acattaacaa aaaaattaat gcaaaagatc
     3241 gtgcagcgat tgccgcagcc cttgagtctg tgaagctgtc tgatatatcg tctaatctga
     3301 acagattcag tcggggactg ggatatgcag gaaaatttac aagtcttgct gactggatca
     3361 ctgagtttgg taaggctgtc cggacagaga actggcgtcc tctttttgtt aaaacagaaa
     3421 ccatcatagc aggcaatgcc gcaacggctc ttgtggcact ggtcttcagt attcttaccg
     3481 gaagcgcttt aggcattatc gggtatggtt tactgatggc tgtcaccggt gcgctgattg
     3541 atgaatcgct tgtggaaaaa gcgaataagt tctggggtat ttaaataaaa tataagacag
     3601 gctgtctatc ttacagacag cctttttata ataacagaaa gaatatatat catacttaac
     3661 ggaagagata atatgaaaac agtacccgta taaacagcaa gcaatcccat ttttccaggt
     3721 gtatcagcaa aaaaaacgct gttccagaaa tcaggccggg taaatttcag agctgtatct
     3781 tcaatatacc attttgcaac cggatacaaa accattccgc agagagaaat acacca
//
LOCUS       DAZOXR010000282         3832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZOXR010000282 DAZOXR010000000
VERSION     DAZOXR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.289"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHUNN_004199"
     CDS             complement(<1..266)
                     /locus_tag="ACHUNN_004199"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0578398.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="ACHUNN_004200"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="ACHUNN_004200"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0578399.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="ACHUNN_004201"
     CDS             complement(726..998)
                     /locus_tag="ACHUNN_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR0578400.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUNN_004202"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUNN_004202"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0578401.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3733)
                     /locus_tag="ACHUNN_004203"
                     /pseudo
     CDS             complement(2694..3733)
                     /locus_tag="ACHUNN_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          932 c          917 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tg
//
LOCUS       DAZOXR010000283         3797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZOXR010000283 DAZOXR010000000
VERSION     DAZOXR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.290"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHUNN_004204"
     CDS             <1..81
                     /locus_tag="ACHUNN_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578402.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHUNN_004205"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHUNN_004205"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0578403.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="ACHUNN_004206"
     CDS             1027..1485
                     /locus_tag="ACHUNN_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0578404.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="ACHUNN_004207"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="ACHUNN_004207"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0578405.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHUNN_004208"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHUNN_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0578406.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="ACHUNN_004209"
     CDS             2691..3368
                     /locus_tag="ACHUNN_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0578407.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHUNN_004210"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHUNN_004210"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0578408.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          952 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAZOXR010000284         3793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZOXR010000284 DAZOXR010000000
VERSION     DAZOXR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.291"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUNN_004211"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUNN_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0578409.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUNN_004212"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUNN_004212"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR0578410.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUNN_004213"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUNN_004213"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR0578411.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          874 a          988 c          915 g         1016 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatcgc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgtg atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgctcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat acatagatat
     3781 taattatgat cgt
//
LOCUS       DAZOXR010000285         3764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZOXR010000285 DAZOXR010000000
VERSION     DAZOXR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.292"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            22..1935
                     /locus_tag="ACHUNN_004214"
     CDS             22..1935
                     /locus_tag="ACHUNN_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0578412.1"
                     /translation="MSFIDYDILSASSSKELREKLAEKIAEGWQPFESPNIVEPSKGG
                     PNAYNQAIVRSGDLSQLPTPVSNEDEEPDYYYVVTIAGQSNSMSYGEGLPMPKTFDRP
                     HPRVKQLARRSNIKPGGFACKYNEIIPADHCLHDVQDMTKFKHPKANDSEYGCVGQGL
                     HIGKKLLPFIPANAGILLVPCGRGGSGFTVTGSEGQYYEESGAIESSLKWGLNTPLYK
                     DIVARTKAALSKNKKNVFLGVVWMQGEADLATGKYSQHNELFLKMVEGFRKELSQYDA
                     QCVFGSAANVPWICGDTTHWWKNKYPKQYEIVYGGYKGKTSQAIYFVPFMKDENDNNV
                     LTNSPTDDPDITSIGYYGSGHRKDNKTWTSESRESHFGSWQRRGIISDRLATALLAHS
                     AKSSAFVNGVTGEIIKQEQPTQPKNDNIVTTPTPRTVISLTGAGGDVATEQGWTVTGA
                     NIQSVENGKAIALTKAVSNETWTISKATDIAQALLENGGKLLCKFKLSGNPDPKKAKF
                     GFGIYYKTSKPVADDISFDGTGSLFLANFFLQTIGGKLNIMMHRTGNNRNVQIDSYGD
                     YDNNWHTLELQYEKGTGTVIPVLDGKAGSKFKLVKDNIALGDEAFVLTDITNNQTHDL
                     AIETLTISINNPT"
     gene            2143..3246
                     /locus_tag="ACHUNN_004215"
     CDS             2143..3246
                     /locus_tag="ACHUNN_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0578413.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAIMISQINNNMIFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELA
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRLNFS"
     gene            complement(3241..3627)
                     /locus_tag="ACHUNN_004216"
     CDS             complement(3241..3627)
                     /locus_tag="ACHUNN_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0578414.1"
                     /translation="MSDKNIEFIESRYAAFIAGLIESSPMSQAQIARLIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
BASE COUNT         1185 a          771 c          808 g         1000 t
ORIGIN      
        1 atcgttaaag gagtaacgat tatgtcattc attgactatg atattttgtc agcatcatca
       61 agcaaagagc ttagagaaaa acttgcggag aaaatcgcag agggttggca acctttcgag
      121 tcaccaaaca ttgttgaacc aagtaagggc ggaccaaatg cgtacaatca agcaattgtg
      181 cgatctggtg atttatcaca attgcccaca ccggtatcaa atgaagatga agagcctgac
      241 tattattatg tagttactat cgcaggtcaa tccaactcaa tgtcatacgg cgaaggactg
      301 cctatgccaa aaacttttga tcggccacat cctcgcgtaa aacagttagc tcgacgcagc
      361 aatattaaac caggtggttt tgcctgtaag tacaatgaaa taattcccgc tgatcactgt
      421 cttcatgatg tacaagacat gactaaattc aagcatccaa aagcaaacga ctctgaatat
      481 ggatgtgttg gtcagggact acacattggc aaaaaactat taccattcat tccagcaaac
      541 gcaggtattt tgctcgttcc ttgcggaaga ggtggttctg gtttcactgt tacaggctct
      601 gaaggccaat attatgaaga gtcaggtgca atcgaatcat ctttaaaatg gggattaaat
      661 actcctttgt ataaggatat tgtagccaga acaaaagcgg ccttaagtaa aaacaaaaag
      721 aatgtatttc ttggtgttgt ctggatgcaa ggcgaagctg atttggcaac tggcaagtac
      781 tcgcagcata acgaactgtt cctcaagatg gtcgaaggtt tcagaaaaga gctatctcaa
      841 tacgacgcac aatgtgtttt cggaagtgct gcaaatgtac cctggatttg tggcgatacg
      901 acccactggt ggaaaaacaa atatccaaaa cagtacgaaa ttgtttatgg tggttataag
      961 ggaaaaacct cacaagcaat ttatttcgtt ccattcatga aagatgagaa tgataataac
     1021 gtattaacca atagccctac agatgatcca gacatcacat ctattggcta ttatggttcc
     1081 ggtcatcgca aggataataa aacatggact tccgagagta gagagagtca ttttggttca
     1141 tggcagcgac gcggaataat atctgatcgt ctcgcaactg cgctgcttgc acattcagcg
     1201 aaatcatctg cttttgtgaa tggcgttaca ggtgagatta ttaaacaaga acaacctact
     1261 caacctaaaa atgacaacat tgtcacaact ccaaccccgc gaacggttat ttccttaact
     1321 ggagctggtg gtgatgtcgc tactgaacaa ggatggactg taactggtgc aaatattcag
     1381 tcagttgaaa acggaaaagc aattgcttta actaaagcgg tttccaacga aacttggacg
     1441 atcagtaaag ctactgacat agcacaggct cttcttgaga atggaggtaa attactttgt
     1501 aagtttaaat tatctggaaa ccctgatcca aaaaaagcta agtttggctt tggtatatat
     1561 tacaaaacca gtaaacctgt tgctgacgat atatcattcg acggtactgg gagcctgttt
     1621 ctggctaact tcttccttca aacaattgga ggcaagttga acattatgat gcacaggaca
     1681 ggcaataatc gaaatgttca gatagactca tatggtgact acgacaacaa ttggcacact
     1741 cttgagttgc aatatgagaa aggcacagga actgtaattc ccgttctcga tggcaaggcg
     1801 gggagtaaat tcaagctggt taaagacaat attgctcttg gtgatgaggc ttttgtttta
     1861 acagatataa caaacaacca aactcatgat cttgctatcg aaacacttac cattagcatc
     1921 aacaacccaa cctaacacaa agtaacagcc aggcatgaaa ttgcctggct tcatatcgaa
     1981 tcaaatattt aaaactagct ctacagaaaa aaatgattcc atactcttta ggtatggaat
     2041 cattttccgc atgaaagatt ttagcttgat tccaaaagaa tcaatgccta atatactggt
     2101 tacttataca ggtgatcagc gtaactaggt gaatgccaat aaatgaaaaa tagctttgac
     2161 agagcacgcg cagcagaaaa cacctcaaaa gaggcaattg agtatctcga aagagcatct
     2221 caaatgcagg ccattatgat ctcacagatc aacaataaca tgatattctc ggacgcattc
     2281 atgttattca ctcgcttatc tctgctgata accagacgtc ggccagagat cgctgttcat
     2341 tgtattttga tacatgtttt gccgcacatt gctgatgtaa aagtaagtga cattaatagg
     2401 ttcatggtta accaactggt caaccctcta atattggatg gcaaaattgt tatgggccgc
     2461 cgcgttttct ctctgatgaa gcagttcctt agctggtgcg ccttccaggg gatgatagac
     2521 gtgtcaccgt taaacgatat gtcactaaac aaagttgccg gtggcgcaaa gcccacacct
     2581 cgcgagcgga agctgaccga cgcagaggta tgggtgttct ggaatatatg ggactacttc
     2641 aatgtgtgcg ctggtacaaa atgggcggcc aggctatgtc ttgtatcagc aagacgacct
     2701 gacgaagtac tgagggctaa aaaaagtgag ttcaatctta agcgtggagt ttggaatcaa
     2761 ggcaagagaa acaagtcagc acgtgagcat tcgctacctt taagcacatt aatgcgcaca
     2821 tgcattgaag agttgttcga atacggtaaa gacagccagt ggctcgtgcc ttcgaataaa
     2881 aaaatcggga aagaccttcc tatgtctaaa gtggcaatag cccaggcatt acgtcgtatt
     2941 ctggaacgac cagaactgat ggagcttgcg ccatttacac cccgagattt gcgccgtact
     3001 gcgcgtagtt acttcccagc attaggcata agccaggagg tatcacgcaa aataatgaac
     3061 cacagtcttg aggggataga tcgggtctac gaccggcacg attatatgga cgagatgcga
     3121 gacgccttag aaagtttctc aacgtacatc gcatcaatcg tagagcaacc ggatttagac
     3181 gaaattgacc acaaattcaa gggagatcgt ctatcaacag agcttattcg tctaaatttt
     3241 tcatagagac tttatggcct caacaacctt ttgtgatgcg cctttctctt taccgaatcg
     3301 ctcgttatat gcagcaagaa cctgtttttc gtcctcgtta agaggagcgg taccttcttt
     3361 gtataaaaat gctgcgagtt caggttggcg ttcttccagc accatcatca taagacgact
     3421 tggctcaata cccagcgcca acgccagcgg acgaacctta tcgataggca aaggaatttt
     3481 accactttta attaaagaaa ggttgttggc atttttataa ccaataagtc tggctatttg
     3541 ggcctgactc ataggtgagg attcaatcag ccctgcgata aaagcagcat agcgactttc
     3601 tataaattca atgtttttgt cagacattat tgcgaccttt gcgcgttaaa ctctctggta
     3661 agtgcttacc gatattacat caaaggttag ggttgtaaag atataatcat tttttctaca
     3721 ggcacaaata ttaaccaatc agcattcatt aatggagaaa aacg
//
LOCUS       DAZOXR010000286         3739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZOXR010000286 DAZOXR010000000
VERSION     DAZOXR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.293"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..402)
                     /locus_tag="ACHUNN_004217"
     CDS             complement(<1..402)
                     /locus_tag="ACHUNN_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0578415.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVKL"
     gene            454..1059
                     /gene="wrbA"
                     /locus_tag="ACHUNN_004218"
     CDS             454..1059
                     /gene="wrbA"
                     /locus_tag="ACHUNN_004218"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0578416.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            1080..1307
                     /locus_tag="ACHUNN_004219"
     CDS             1080..1307
                     /locus_tag="ACHUNN_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0578417.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(1345..2586)
                     /gene="agp"
                     /locus_tag="ACHUNN_004220"
     CDS             complement(1345..2586)
                     /gene="agp"
                     /locus_tag="ACHUNN_004220"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0578418.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2880..3739)
                     /locus_tag="ACHUNN_004221"
                     /pseudo
     CDS             complement(2880..3739)
                     /locus_tag="ACHUNN_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          998 a          898 c          825 g         1018 t
ORIGIN      
        1 caacttcaca gggtctgtta aggctgactt gtcgtagagt tcaccatcac ccagagtcag
       61 tatagagacc cccttcctca gtatctgcct gacatcatct atagctgtgt cgatatcagc
      121 cctactgaag cggtcaaagt tctctacaac aatcaaggaa ccttctggaa tagccccaga
      181 cataacattt tcaaacatac gtctgaagtc agaaccttga ttcttatgtt gctggtgaaa
      241 accagattta cccttatcaa catatctgtg agcaggaaga aggtaataat cgtttttgtg
      301 cttagagtag aaagcatcaa tgaggtttgt ttgccgtctt aaggagtctc ctgagtcttg
      361 ctctttagta ctccacctga tgtatgtcac tagcagcttc atatcttcgg actatgctta
      421 tctaagtggt tcattgaaca ggagaacaat gatatgagtg ttaaggcaag aatactagtg
      481 ctttattatt ccatgtacgg acatattgaa acgatggcac gcgcagtcgc tgagggtgca
      541 agcaaagtcg atggcgcaga agttgtcgtt aagcgtgtac cggaaaccat gccgccgcaa
      601 ttatttgaaa aagcaggcgg taaaacgcaa actgcaccgg ttgcaacccc gcaagaactg
      661 gccgattacg acgccattat ttttggtaca cctacccgct ttggcaacat gtccggtcaa
      721 atgcgtacct tcctcgacca gacgggcggc ctgtgggctt ccggcgcact atacggaaaa
      781 ctggcgagcg tctttagttc caccggtact ggcggcggtc aggaacaaac tattacttca
      841 acctggacga cccttgcgca tcacggcatg gtgattgtcc ccattggcta cgcagcgcag
      901 gaattatttg acgtttcaca ggttcgcggc ggtacgccgt acggcgcaac caccatcgca
      961 ggcggtgacg gctcacgcca gccaagccag gaagaactgt ctattgctcg ttatcaaggg
     1021 gaatatgtcg caggtctggc agttaaactt aacggctaat cttcaacagg aggatacgca
     1081 tgccaactca agaagcgaaa gctcatcacg tcggtgaatg ggcatctctg cgcaatacgt
     1141 cgccggaaat agccgaggcc attttcgaag tcgccgggta cgacgaaaaa atggcagaaa
     1201 agatttggga agaaggtagc gatgaagtgt tagtcaaagc ctttgctaag gccgataaag
     1261 attcgctttt ttggggcgaa cagaccatcg aacgtaaaaa cgtttaacca gcgactcccc
     1321 cgcttctcgc gggggagttt tctgttattt caccgcttca ttcaacacgc tatcaaattt
     1381 atccatcggg cagaaaccgt tggcatctat cgggcaaccg cttaattcca gcgtcacacg
     1441 ctgagcaggt gcctgcaggg ttaacgcatc ggcattacgt aactgttccg cactctgata
     1501 cacatattca attttcatca aatcgcgatt ggctttgctg tcatgccaac gctggaaaac
     1561 gattttgccg ccaatcggcg tgcgttcgtt ctggtcatgc aactgatacg gtttgaaatc
     1621 cagcgccgtt aacagagagg caatgttgga gtcgtgccca accaacactg taattttcgg
     1681 tgcgctggtg cgatcggtga ccagagcttt gtcgatataa ctgaccagcg gtttcgcaac
     1741 attgcgcgcc acttccggtg aggtaaacag gctgtcctgg tagccgtttt taagcttcga
     1801 caacaccttc cactgctggt cagatttgat ttctccccag gccacctgat ccatcggaaa
     1861 accttcgtaa tattgcaaag taaacgcatc taccagcgag ttgccgactt tcagcggccc
     1921 ggaaacacct ggttcttgtt gatacttggc gctaaaggta tttttgccat ccaccagcga
     1981 acactgttgt ttctctttac aggcagggga atctttatag ttaacgattt tttccagtag
     2041 ctggtagctg tcggtaagct ggagtttgct gagctctttc tccattgccg ccaccgcctg
     2101 ttcactgaat gcggcggaat catcggtgat caccgggtta aaggttgggt ccatagtgcc
     2161 cattttttcc tggtgatgca caggaatatc acaccccggg aatgcgccgg taataaagaa
     2221 ctgtgcggtc gcaacggtac gttgcagact attggcatag gcataaacgg tgtccggcgg
     2281 cgggcattcc cccgatttca ccatcccctg ctctgccagc cattcacgca tgtaatggcc
     2341 catatacact tcgagcacgc cacctttggt ggtgagttgc ccaccgggga cgtcccattc
     2401 tggccattta ttcggcgtcg actgctccag cacactgcca ttgttcgcca gcggcgcacg
     2461 taagttatgg cggctcatca tgagcacttg ctgtagctga tagccttccg gtacggtttg
     2521 tgcctgagca tttgaagcga gtaaaactat ccctgccaca gttgcggcga ttagcgtttt
     2581 gttcattcct ggcacctctt ttgttatcaa tcattaaaga gtgtgacaga aatatgactt
     2641 ttttccggga acaggttcgc aaaatggctt gttgaacgcc tgtttttcta tatacatctt
     2701 caaaaacagg caaggtttga cgcaaactat gcctgattag cccctgcctc gcgtacgccg
     2761 ctgataattg cacctgtgga taaacagaat atcagcggcg ttactaatac agaggcaata
     2821 gtaccaataa taagagaagt gaggctaata acaatggttt ttagcacatg cctttgtcat
     2881 taaattgcat aacgagttaa aaaatcaatt tgcgcattcc tttccttatt gtttaactcg
     2941 taagcctgag caaaaagttc aggagggagg taaacgtcat aatgattgcc agtgtttatt
     3001 aaatacccat actcaaggtc gtcatctgtt gaaaaggatt cattccctaa ggagattgat
     3061 gccacattgc tgtaaacaat aaataatgag caaatgctat ctgaaagagc agtgaggaaa
     3121 tttttcacct cattaacctt ctgctctggg tcggtgtcta aaacaaggcg tgccttgtta
     3181 tcattaaata taaattcggg taaatgcagt atgcattttt tgtcattcaa gcaactatca
     3241 ataaattctt ttttaaaaga caaaaaactc catgataaaa aaccaccctg cccctgaatt
     3301 ttgttgtcag attgtagatt aacgccaaat aacgtcttta catcttccct gagattatag
     3361 aatgccgtat caacaagagc ctcgatcgtt tgtttatcaa caattttaat aaactcttcg
     3421 tatacttgct tttgcgtctt atggccgccc agcgcccatg aatactcatg ttcttttaaa
     3481 accagtatct aaatcagata ttcccggcgt atttatctgt tctggctgga tactcatatt
     3541 ttcatcatct aaacaatact cagaaaaaat gtcatcacac gcagtaatgt tttcaatata
     3601 ttccataaag ccataccaat aataaccttt aaaatctgtg atagactctg aacctctgcc
     3661 cggcaaggac actctaacaa tatcaacatt ttgaacatat tgtatagtat atgtaatacc
     3721 tcctgaaacc cctgaataa
//
LOCUS       DAZOXR010000287         3735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZOXR010000287 DAZOXR010000000
VERSION     DAZOXR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.294"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHUNN_004222"
     CDS             complement(<1..181)
                     /locus_tag="ACHUNN_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR0578419.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHUNN_004223"
     CDS             425..682
                     /locus_tag="ACHUNN_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR0578420.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUNN_004224"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUNN_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0578421.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUNN_004225"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUNN_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0578422.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUNN_004226"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUNN_004226"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0578423.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3735)
                     /locus_tag="ACHUNN_004227"
     CDS             complement(3307..>3735)
                     /locus_tag="ACHUNN_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0578424.1"
                     /translation="KFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          954 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aattt
//
LOCUS       DAZOXR010000288         3723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZOXR010000288 DAZOXR010000000
VERSION     DAZOXR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.295"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..549
                     /gene="excA"
                     /locus_tag="ACHUNN_004228"
     CDS             <1..549
                     /gene="excA"
                     /locus_tag="ACHUNN_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR0578425.1"
                     /translation="ILFGVLSITSSRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRK
                     DKIKKIVAEIKNTGFYSPDKEYEGLSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGF
                     DIDNFTRTVTDAKTLEIYTKYINIPMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFP
                     RLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(621..830)
                     /locus_tag="ACHUNN_004229"
     CDS             complement(621..830)
                     /locus_tag="ACHUNN_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFR0578426.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            1222..1398
                     /locus_tag="ACHUNN_004230"
     CDS             1222..1398
                     /locus_tag="ACHUNN_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578427.1"
                     /translation="MNLVDAFVKKVISGPYEEYGKWWIDVEYISWGVPGKTRLMFDSK
                     EQALEVKEGYKFLT"
     gene            complement(1463..1558)
                     /gene="dqlB"
                     /locus_tag="ACHUNN_004231"
     CDS             complement(1463..1558)
                     /gene="dqlB"
                     /locus_tag="ACHUNN_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0578428.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELVD"
     gene            2057..2308
                     /locus_tag="ACHUNN_004232"
     CDS             2057..2308
                     /locus_tag="ACHUNN_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578429.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(2380..2532)
                     /locus_tag="ACHUNN_004233"
     CDS             complement(2380..2532)
                     /locus_tag="ACHUNN_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0578430.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            3101..>3723
                     /locus_tag="ACHUNN_004234"
     CDS             3101..>3723
                     /locus_tag="ACHUNN_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578431.1"
                     /translation="MRSRYVDRIIYMKKLLIRLIPDAIYEALEKTALHSERSLEAQAR
                     YILSCSVDNEKQLTGGERYQREITARLNQALSEANEVITAINLVPARIAEQLGHHDAI
                     ESENWFTGNAVPSFTELDELSDIFGCSPDWLKFGENVPYPKSSKGRINWNRGGEKDID
                     ALLEPDNKGRKVSSIHIFRVNESGNILILREFENSITTDFFSTNLYLS"
BASE COUNT         1175 a          839 c          758 g          951 t
ORIGIN      
        1 attcttttcg gcgtgctctc cattacatct tcaagatatg tgacgcaaga agattatatt
       61 tatacttgcg tctgtctgtt tttactaatt gcccctgcaa tattgatgta ttcccgagca
      121 agctcaagaa aagacaaaat aaaaaaaatt gtcgcggaga taaaaaacac cggtttttat
      181 tcacctgaca aagaatatga gggactatct tttactcagg gcgtctattt tggcgtggat
      241 accaaaaaag gcactatgct gtatgctcgt gcttaccctg gaaatataat ggatattatc
      301 ggctttgaca ttgacaactt cacacgcact gtgaccgacg ccaagacact agagatttat
      361 acaaaataca taaatatacc aatggtctca attccgtcag gatgcatcca tcccaaaatg
      421 atggcggata caatgcatgc aatggcagaa agaggatatg attaccctgt cgactttcct
      481 cgcctaattc aggagaagcg taaggagtgg gagcagattg caggcatgcc tgtagccgaa
      541 gtattttaag ataagctccc tccataacag ccggttcaaa tgagccggct tttgttcatt
      601 acaacagata acttatgccg ttaccgataa attcgaccaa gtgctttaat cgcagcaatt
      661 tttatttcca tatcatcact ttgactcaat ctatgtaatt cacgagtaat gttctccgcc
      721 tgacaccggc cttcacctaa tgcatagatc gcagcaagtg taacgtcttt tgatttactc
      781 ttcatcattt caaacaagaa agtattagct atcgcaggag gcatgctcat aatcaatcct
      841 cttcatgaaa taaacaatgg cactcactta caaacaggag tccactatcg caagcgactc
      901 aacttagcaa tatctctcaa tttgaatgaa aaaaacagat ggcatgtgac agggaaccgt
      961 ccgcatgatg tccgtgacca tttaacccca taagctaacc ttgtcacaaa cgggcgcaga
     1021 acagcctgcc agacagacaa caaaacaaaa gtccaagata aaacatgaat accggtatct
     1081 caatgcgaag aaataatctc cgtagaaacc cccatgagaa aaatatcata tgggagaaaa
     1141 tcgcaattat cacagccagc gcattcatta ctttcgctgt gttctggcat taattatttt
     1201 caacacaaaa aggaaaaaac aatgaatctg gtagacgcct ttgtcaaaaa agtaatatcc
     1261 ggtccatatg aagaatatgg aaaatggtgg atagatgtcg aatacatttc atggggtgtt
     1321 cccgggaaaa caagactgat gtttgactca aaggagcagg cactggaagt taaggaaggg
     1381 tacaaatttt taacgtgaga tctctttttg ttaaagggca ggaagcggta actccctgcc
     1441 cttagttacg tttccaggct tatcagtcga caagttcccg gatcagttcc agaacagcaa
     1501 tcagcgcgtt tgcaacggca actactgccc tgagaacgat gattaacaga ttaatcatcc
     1561 gcttctccat cactggagga aatcaccgtt gcttactctt acattgcctg tcacaacggc
     1621 actttcagtg ttagttgaaa agagacttcc tcctgccaga gcactgaaca cagcaccacc
     1681 tgtttgttca gccaggactt aaaatcttgc atccatagtc ccgatggata tcacggcaga
     1741 agtgtcgcca gacagagcta acctgcggat tataaggatg gttgctatca aatgaacagc
     1801 tcgctattat aagaacgtta gttgaaaaga gatttcctcc ggtcagggcc acccactctg
     1861 taacggaaca gtaaaaaaac cgacctcacc agtcggtttt tttacatctg ttgtttctca
     1921 aaatccgtcc gacatgcccg tcacaccggg gaaactcatg ctctgaatca gaaccaacca
     1981 tctcaacttt gccgtctcct gaaaaaactg gcacattcag ttcgataact acccataaat
     2041 acagagagaa aatccgatgt cagaaaagtg gagtaaagaa ttcgactctt gctttcaggt
     2101 ggggagcata gaagaaattg cagatgctct cgagaaacgc agcggaagcg aggaaaatgc
     2161 gttagccatg ctggcaatga cagccttcac actcatgacc cgacgtggta tctgcgaaat
     2221 gcagaacgtg gcccccgatg gcaaaggcat tcggctggag ctaatcggct tcagggaaaa
     2281 tgaaacaata ccggatacgc ttcattgaag aagaaagggc gcaatgaggt cagccttcgc
     2341 aacaaagcgg cggcagaaaa cctgccgccg tgttgcccgt taacgtttaa cttcgtaggc
     2401 taacgttgcc acaagcactg tgtttccctg ctggagccgg agtccgcaaa gcgaatccct
     2461 caccatccag acaaaacaca gaatcgtcac acagatgacg attaacatca ttaaaaacgt
     2521 tcgctgtggc atcatagccc ctccattgcc ttacggcggt tatcgggcta ctataatgtt
     2581 gtctaggcat tacagtagag cccttggttg attgataaaa aaatctccag gggctttctg
     2641 ctgtataccg tccacaaaac gctttcggca tacagcctca agcgcccgca gctattctaa
     2701 cgactttcgt atcggtaaca atccttttaa ccagccccac acatctttcg gacatggtga
     2761 gtacaaaagg ctgcgctctc gaacatttga cattgaaaat gattcaggtg acagcctgaa
     2821 tgtagcagga gtccccgtcc ttctggcata gaagatgtca acgggctact ataatgtcgt
     2881 tcacgcatta cagtagagcc cttggttgat tgacaaaaat ctccaggggc tttctgctgt
     2941 atatggttta gttaacaaag agagccagtg ttccactgag caaaaccagt agctattcat
     3001 gcttacaatt atcttctcca tatctgaaat aactcaaaca gcccaataga aacatattgt
     3061 tacatctaag atcttgaaaa gacctctttg agaaatcaat atgagatcac ggtatgttga
     3121 taggataata tatatgaaga aactattgat tcgattaata cctgatgcaa tatatgaggc
     3181 tctggaaaag acagctctgc attctgaacg ctccctggaa gcacaggcca gatatatcct
     3241 tagttgctcc gtagataatg aaaaacaatt aactggtggt gaacgctatc aaagagaaat
     3301 aaccgcgcgt cttaatcaag cgttatcaga agctaatgag gtcataacag ccattaatct
     3361 tgttcccgca agaattgcag aacaattagg ccatcatgac gccatagaat ccgaaaattg
     3421 gttcaccgga aatgctgttc ctagctttac agagcttgac gaattaagcg atatctttgg
     3481 atgctctcca gattggttaa agtttggcga aaatgtacct tatccaaaga gttctaaagg
     3541 aaggataaac tggaatcgtg gcggagaaaa agatatcgat gcacttttag agcctgacaa
     3601 caaaggtcgt aaagtctcat ctattcatat tttcagggta aatgaatctg gcaatatact
     3661 aatactgaga gaatttgaaa acagcataac tacagatttt ttctcaacaa acctttactt
     3721 atc
//
LOCUS       DAZOXR010000289         3715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZOXR010000289 DAZOXR010000000
VERSION     DAZOXR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.296"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(108..1472)
                     /locus_tag="ACHUNN_004235"
                     /pseudo
     CDS             complement(108..1472)
                     /locus_tag="ACHUNN_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1537..1656)
                     /locus_tag="ACHUNN_004236"
     CDS             complement(1537..1656)
                     /locus_tag="ACHUNN_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0578432.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1880..2038)
                     /locus_tag="ACHUNN_004237"
                     /pseudo
     CDS             complement(1880..2038)
                     /locus_tag="ACHUNN_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2383..>3715)
                     /locus_tag="ACHUNN_004238"
     CDS             complement(2383..>3715)
                     /locus_tag="ACHUNN_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR0578433.1"
                     /translation="RGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLK
                     VFNVYFDNNEELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQ
                     FIECAKRNEQTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVS
                     PVTKNSQQVKGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEF
                     RGVLIDKNGNCSERLFNFYECCYKFLPRAQPQDKVESYNSALQAFSIFRSSTLNNNDV
                     GFNFKLFPEVKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCF
                     FSGQNFSNYDIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRN
                     NKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1035 a          836 c          663 g         1181 t
ORIGIN      
        1 cctaagacat caatcatctg ctctccaatg actagtctaa aaactagtat taagactatc
       61 acttaaataa gtgatactgg ttgtctggag attcaggggg ccagtctagc aactaaagca
      121 ctgcccgtct cctggttaac cccatgtgct ataccgccgg tatagacaat cagcgagcct
      181 ccggcgctaa tattgctgcc agttgccgta ccatctttct caacaacctg ccggctcccg
      241 gtggaaatat ttgtcgtgtc agctttcccg ccgctcttga tattttgtgt tccgctgttg
      301 atattggtac ccgtggcaat accatgatta ttaatattct gtgtaccacc attgattatg
      361 gtattggtcg cgtttctggt aacatccatt accccgccgt tatctattcg ggtcgcatca
      421 gctttagcat tcgttaaaac tgacattgtt cctttatctt taataatcgt cttatttgcc
      481 gaaccatatg cgtttatgtc taaatgacca ccgttttcca gcaacacatt gtctgccacg
      541 tgattgtgga tggagaatgc accttcacta ttcgtaccgc tcaccgtcgt accgttagtg
      601 tttgttttta aaattgcacc atcgtgctgg gtaacatttg ttgccgtacc accactaaca
      661 tcaagcacgc caccggaata aacttcaata acatccgagg agctggtgta atcaacaatt
      721 tgcgtaccac cagaatagat ctgagtattt tttgccgttg acttattatt cagagactga
      781 gttccacctt caatcgtcgt gtccagcgca tggctttcat atactctttg ctcaccgcca
      841 tttttaatgg tggttgttcc tactgtgctc tgttcaacat actgtcgtcc accatttatg
      901 attgtgttcg ttgccatact tccttgcgtg atgtcctgat aaccggactt atttatcgtt
      961 gtaccatcaa catgcccctg agttgttact gtctgccctc caccatcaat gatggttcca
     1021 ttcgcagtcc ccccacttat gtgtgaatta ccacccttaa ttgtcgttcc attgctgata
     1081 ccgcctgtat aaacgtcctg attgccactg tcgattatcg taccggtgga aatgcccccg
     1141 tcatgaattg tctgtcttcc tccgttaatg gttgtattat tagactgccc catataattg
     1201 ttattccttc caacatcttg atatccacca gattcaataa gacttccatt agatactccg
     1261 ccataaacac tctgctggcc atggttgata atatgagtgt tatttgttgt acctcgttca
     1321 tctactcgtt ggttgccatc tacagtctca tcgtttacca caccaataac attagtagta
     1381 aaggccgcca tcacgggcgg ggcatatatc aaggcagata tcaataagga aagtactgag
     1441 cggcgacaat aatggggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact
     1501 gagtagcgtt taagcgactt tagctctgct gcaacatcag cccgcaggca ccagaccggg
     1561 gggttcatcc tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat
     1621 gatgaaatga tgatgttcca cataagaaag aggcattttt taaacacagt gcgctgaagt
     1681 gtgattggat aaaaaagtca atctattcag gaaatacggg cgtattcttt tctttcgaca
     1741 atcaggccgt cggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct
     1801 cacataaaaa tcacacaata actttgagat cgcagattct tttactttta cagcattcgt
     1861 ccccccattg ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgtttgc
     1921 acagctcctc caacagcacg acaagatgcc acatactgcg ccccagtcgg tgcggtttac
     1981 agactacccg tgcgtccacc gccgataatg tcctgagcag ttttttcagt ccggacctcc
     2041 tgccacggtt ttacgttgaa gaggtaaccc tgagcacgca gttcttcagt caggcgtggt
     2101 gcaccgtaac gctgttattg ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt
     2161 tgaaccagac attagatgaa atatttcaac caattacagc accaatttaa actcagtaaa
     2221 tcacagcaca gatcaagggg ttacgtgaaa gcgtagcccc tttttctttg gtagtggcag
     2281 caaaatggtt gtagtgtaaa aaataatccc gtttaatcaa tcaataatac atattgtttc
     2341 aatctacgtt attatctctt tgtaaaaata gccatttatt aatcattgaa aactgctttt
     2401 agaacttgat acaacgggac tagtcacaac aggactattc tcaacgggat catcctcaga
     2461 ggaactatca tcaaagtcat catccataaa taaaatatca tcgaatggtg ccacgcccgt
     2521 gatgagtttt attttattat tacgatcagt caagactcca cttaaaccgt tttcgctcac
     2581 aggttttaat gatttttcat tactcttgtt gtaagcaggc gcattaaaaa tacacggagt
     2641 atcaagatca aacaatgacg ttctccagtt cacatattga atatcatagt tactgaagtt
     2701 ctgtccagaa aagaagcatc ccttaaaatc caatccacct aaattatata aaccatcctc
     2761 ttctttttgg agagtaatgt taattctggc tatctcccgg acaaaacttc cttttttgta
     2821 tttgaatacc gtttcaagat tttcaccaga cagcttgact tctgggaata atttaaaatt
     2881 aaaccctaca tcattattat tcaaagtaga tgaacgaaag atggaaaaag cttgtagtgc
     2941 tgaattatag ctttcgactt tatcttgagg ctgcgctctt ggtaaaaact tatagcagca
     3001 ctcataaaaa ttgaataaac gttctgaaca attcccattt ttatcaatta ataccccccg
     3061 gaattctttc acaaaggcat ccgttatttt ttgaatatct atggtgtgtt cctttgactc
     3121 tgacaaagat ggagtctgtt gaagtgcatt gctcatctgg ttaaggaaca tctgcatttc
     3181 tttaaattta tctccatagt ggcctttaac ttgctgagag tttttagtta caggtgaaac
     3241 cttatctatt tggttgtcat ataaattatg tggttccata cagacatttg gttgtgtttc
     3301 taaaataaca ctacgtgtac tacctggctt aaatgaatca acatgaaaat cactttttct
     3361 tatattatcg aatagggtct gttcatttct cttagcgcat tcaataaact gatcaacatt
     3421 atttttattc gatgacaggt cagtttcaga aaccgcattt tctttgttct ccagctttag
     3481 tttaatgaga agatcttccc agacctgctt gcttaaacat actacgtcag gctcaccatc
     3541 agtaactaac tcttcattat tatcaaagta tacgttgaat acttttaagt tattttcatc
     3601 aacatcatat ttaatacgtt ttaattgctc tccagatccc ataatgacaa aggcgttgcc
     3661 ttcatgatat atacattcag acatcatttt ttgtaaagtt tcaagagcac cgcga
//
LOCUS       DAZOXR010000290         3674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZOXR010000290 DAZOXR010000000
VERSION     DAZOXR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.297"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHUNN_004239"
     CDS             <1..95
                     /locus_tag="ACHUNN_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0578434.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="ACHUNN_004240"
     CDS             244..1491
                     /locus_tag="ACHUNN_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR0578435.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHUNN_004241"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHUNN_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR0578436.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="ACHUNN_004242"
     CDS             complement(2343..2687)
                     /locus_tag="ACHUNN_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578437.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="ACHUNN_004243"
     CDS             2929..3531
                     /locus_tag="ACHUNN_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578438.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctagggt tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatgga gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAZOXR010000291         3641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZOXR010000291 DAZOXR010000000
VERSION     DAZOXR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.298"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(36..80)
                     /locus_tag="ACHUNN_004244"
     CDS             complement(36..80)
                     /locus_tag="ACHUNN_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578439.1"
                     /translation="MKRNDKILHYEGLN"
     gene            199..1923
                     /gene="ilvI"
                     /locus_tag="ACHUNN_004245"
     CDS             199..1923
                     /gene="ilvI"
                     /locus_tag="ACHUNN_004245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0578440.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1926..2417
                     /gene="ilvN"
                     /locus_tag="ACHUNN_004246"
     CDS             1926..2417
                     /gene="ilvN"
                     /locus_tag="ACHUNN_004246"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0578441.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2429..2515
                     /locus_tag="ACHUNN_004247"
     CDS             2429..2515
                     /locus_tag="ACHUNN_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0578442.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2597..3601
                     /gene="cra"
                     /locus_tag="ACHUNN_004248"
     CDS             2597..3601
                     /gene="cra"
                     /locus_tag="ACHUNN_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0578443.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          848 a          867 c         1009 g          917 t
ORIGIN      
        1 attgctgtaa gttgtgggat tcagccgatt tattatcaat ttaatccttc gtaatggagg
       61 attttatcgt ttcttttcac ctttcctcct gtttattctt attaccctgc gtttatgtct
      121 ctggctgcca attgcttaag caagatcgga cggttaatgt gttttacaca ttttttccgt
      181 caaacagtga ggcaggccat ggagatgttg tctggagccg agatggtcgt ccgatcgctt
      241 atcgatcagg gcgttaaaca agtattcggt tatcccggag gcgcagtcct tgatatttac
      301 gatgcattgc ataccgtggg tggtatcgat catgttttag ttcgtcatga gcaggcggca
      361 gtgcatatgg ccgatggtct ggcgcgcgcg accggggaag tcggcgtcgt gctggtaacg
      421 tcgggtccag gggcgaccaa tgcgattact ggcatcgcca ccgcttatat ggattccatt
      481 ccattagttg tcctttccgg gcaggtagcg acctcgttga taggttacga tgcctttcag
      541 gagtgcgaca tggtggggat ttcgcgacca gtggttaaac acagttttct ggttaagcaa
      601 acggaagaca ttccgcaggt gctgaaaaag gctttctggc tggcggcaag tggtcgtcct
      661 ggaccagtag tcgttgattt accgaaagat attcttaatc cggcgaacaa attaccctat
      721 gtctggccgg agtcggtcag tatgcgttct tacaatccca ctactaccgg acataaaggg
      781 caaattaagc gcgctctgca aacgctggta gcggcaaaaa aaccggttgt ctacgtaggc
      841 ggtggggcaa tcacggcggg ctgccatcag cagttgaaag aaacggtgga ggcgttgaat
      901 ctgcccgttg tttcctcatt gatggggctg ggagcgtttc cggcaacgca tcgtcaggca
      961 ctgggcatgc tgggaatgca cggtacctac gaagccaata tgacgatgca taacgcggat
     1021 gtgattttcg ccgtcggggt acgatttgat gaccgaacga cgaacaatct ggcaaagtac
     1081 tgcccaaatg ccactgttct gcatatcgat attgatccta cttccatttc taaaaccgtg
     1141 actgcggata tcccgattgt gggggatgct cgccaggtcc tcgaacaaat gcttgaactc
     1201 ttgtcgcaag aatccgccca tcaaccactg gatgagatcc gcgactggtg gcagcaaatt
     1261 gaacagtggc gcgctcgtca gtgcctgaaa tatgacactc acagtgaaaa gattaaaccg
     1321 caggcggtga tcgagactct ttggcggttg acgaagggag acgcttacgt gacgtccgat
     1381 gtcgggcagc accagatgtt tgctgcactt tattatccat tcgacaaacc gcgtcgctgg
     1441 atcaattccg gtggcctcgg cacgatgggt tttggtttac ctgcggcact gggcgtcaaa
     1501 atggcgttgc cagaagaaac cgtggtttgc gtcactggcg acggcagtat tcagatgaac
     1561 atccaggaac tgtctaccgc gttgcaatac gagttgcccg tactggtggt gaatctcaat
     1621 aaccgctatc tggggatggt gaagcagtgg caggacatga tctattccgg ccgtcattca
     1681 caatcttata tgcaatcgct acccgatttc gtccgtctgg cggaagccta tggtcacgtc
     1741 gggatccaga tttctcatcc gcatgagctg gaaagcaaac ttagcgaggc gctggaacag
     1801 gtgcacaata atcgcctggt gtttgttgat gttaccgtcg atggcagcga gcacgtctac
     1861 ccgatgcaga ttcgcggggg cggaatggat gaaatgtggt taagcaaaac ggagagaacc
     1921 tgattatgcg ccggatatta tcagtcttac tcgaaaatga atcaggcgcg ttatcccgcg
     1981 tgattggcct tttttcccag cgtggctaca acattgaaag cctgaccgtt gcgtcaaccg
     2041 atgatccgac attatcgcgt atgaccatcc agaccgtggg cgatgaaaaa gtacttgagc
     2101 agatcgaaaa gcaattacac aagctggtcg atgtcttgcg cgtgagtgag ttggggcagg
     2161 gcgcgcatgt tgagcgggaa atcatgctgg tgaaaattca ggccagcggt tacgggcgtg
     2221 acgaagtgaa acgtaatacg gaaatattcc gtgggcaaat tatcgatgtc acaccctcgc
     2281 tttataccgt tcaattagca ggcaccagcg ataagcttga tgcattttta gcatcgattc
     2341 gcgaggtggc gaaaattgtg gaagttgctc gttctggtgt ggtcggactt tcgcgcggcg
     2401 ataaaataat gcgttgagaa tgatctgaat gcgcaatttg cagcccaaca tgtcacgttg
     2461 ggcttttttt gcgaaatcag tgggaacctg gaataaaagc agttgccgca gttaatttta
     2521 tgcgcttaga tgttaatgaa tttaacccat accagtacaa tggctatggt ttttacattt
     2581 tacgcaaggg gcaattgtga aactggatga aatcgctcgg ctggcgggag tgtcgcggac
     2641 cactgcaagc tatgttatta acggcaaagc gaagcaatac cgtgtgagcg acaaaaccgt
     2701 tgaaaaagtc atggctgtgg tgcgtgagca caattaccac ccgaacgccg tggcagctgg
     2761 gcttcgtgct ggacgcacac gttctattgg tcttgtgatc cccgatctgg agaacaccag
     2821 ctatacccgc atcgctaact atcttgaacg ccaggcgcgg caacggggtt atcaactgct
     2881 aattgcctgc tcagaagatc agccagacaa cgaaatgcgc tgtattgagc accttttaca
     2941 gcgtcaggtc gatgccatta ttgtttcgac gtcgttgcct cctgagcatc ctttttatca
     3001 acgctgggct aacgacccgt tcccgattgt cgcgctggac cgcgccctcg atcgtgaaca
     3061 cttcaccagc gtggttggtg ccgatcagga tgatgccgaa atgctggcgg aagagttacg
     3121 taagtttccc gccgagacgg tgctttatct tggcgcgctg ccggagcttt ctgtcagctt
     3181 cctgcgtgaa caaggtttcc gtactgcctg gaaagatgat ccgcgcgaag tgcatttcct
     3241 gtatgccaac agctatgagc gggaggtggc tgcccagtta ttcgaaaaat ggctggaaac
     3301 gcatccgatg ccgcaggcgc tgttcacaac gtcgtttgcg ttgttgcaag gggtgatgga
     3361 tgtcacgcta cgtcgcgatg gtaaactgcc ttctgacctg gcaattgcca cctttggcga
     3421 taacgaactg ctcgacttct tacagtgccc ggtgctggca gtggctcaac gtcaccgcga
     3481 tgtcgcagag cgtgtgctgg agattgtcct ggcaagcctg gacgaaccgc gtaagccaaa
     3541 acctggttta acgcgcatta aacgtaatct ctatcgccgc ggtgtgctca gccgtagcta
     3601 agccgcgaac aaaaatacgc gccaggtgaa tttccctctg g
//
LOCUS       DAZOXR010000292         3613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZOXR010000292 DAZOXR010000000
VERSION     DAZOXR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.299"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="ACHUNN_004249"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="ACHUNN_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR0578444.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="ACHUNN_004250"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="ACHUNN_004250"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0578445.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..>3613
                     /gene="araH"
                     /locus_tag="ACHUNN_004251"
     CDS             3028..>3613
                     /gene="araH"
                     /locus_tag="ACHUNN_004251"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0578446.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
BASE COUNT          892 a          836 c          973 g          912 t
ORIGIN      
        1 taatgagcac agtgataatt aatggcgaaa tgaatgacgt gcattttcca catctttgag
       61 ttgcggttat tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt
      121 aaaaagaaga catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg
      181 gattaatctg ctgtgcattc gacaatttgt ctgacaaatt ggctttccct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacggtcact tatcttttag ataaaaggta
      361 atgctttgtt ttccgattaa tttaacgaaa gtcattcgtt tttgccctac acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggttttc tggtgaagca
      541 accggaagag ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgag gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga
      661 cagcctggct gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg
      721 ctctgccatc gtcgcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac
      841 taaaattggc gaacgtcagg gtcaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggcgattac cgccaacgaa ctggatacgg cacgtcgccg
      961 tacaacgggt tctatggatg cgctgaaagc ggccggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagctgcc gatatcatcg gcattggcat
     1201 taacggtgtg gatgcgttga gcgaactgtc taaagcacag gcaaccggct tctacggttc
     1261 cctgctgcca agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt
     1321 agcaaaagac gttgaaccgc caaaatttac cgaagttacc gacgtggtgc tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa
     1741 cgcgggtgtg gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa
     1861 ttatgaggcg ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaggcgc tggcgcgtaa
     1981 cgctaaaatt atcgcctttg atgagccaac cagctccctt tctgcccgcg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccatcact gtctttaaag atggacgtta
     2161 tgtcaaaacc tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt
     2221 cgggcgcgac attggcgata tctacggctg gcaacctcgt agttatggcg aggagcgcct
     2281 gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggtgaaatt gttgggctgt ttggtctggt aggggcgggg cgtagcgaat taatgaaagg
     2401 cttgtttggc gggacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcccg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 caaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 gcacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatca tgaatctctc
     2701 aggcggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcagcattga tgttggtgct aagcacgaaa tatataacgt
     2821 aatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc ggattgtggt gatgcgggaa ggtgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg ttctggcgca
     3061 cctaagtcgt cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtgctct ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg
     3181 aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc
     3241 cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc
     3301 acggcggtgg ttatcaacct gactgaaagc ctgtggattg gcgtggcagc ggggttgctg
     3361 cttggcattc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct
     3421 ctgatcacaa cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc taactggttc
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgttgctg
     3601 aataaaacca cct
//
LOCUS       DAZOXR010000293         3577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZOXR010000293 DAZOXR010000000
VERSION     DAZOXR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.300"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUNN_004252"
     CDS             <1..196
                     /locus_tag="ACHUNN_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578447.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHUNN_004253"
     CDS             207..374
                     /locus_tag="ACHUNN_004253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578448.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHUNN_004254"
     CDS             371..715
                     /locus_tag="ACHUNN_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578449.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUNN_004255"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUNN_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0578450.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1469
                     /locus_tag="ACHUNN_004256"
                     /pseudo
     CDS             1383..1469
                     /locus_tag="ACHUNN_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1466..1738
                     /locus_tag="ACHUNN_004257"
     CDS             1466..1738
                     /locus_tag="ACHUNN_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578451.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1740..2798
                     /locus_tag="ACHUNN_004258"
     CDS             1740..2798
                     /locus_tag="ACHUNN_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0578452.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="ACHUNN_004259"
     CDS             2799..3179
                     /locus_tag="ACHUNN_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0578453.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3577
                     /locus_tag="ACHUNN_004260"
     CDS             3176..>3577
                     /locus_tag="ACHUNN_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0578454.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          968 a          808 c          980 g          821 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZOXR010000294         3569 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZOXR010000294 DAZOXR010000000
VERSION     DAZOXR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.301"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(63..1460)
                     /gene="ccp"
                     /locus_tag="ACHUNN_004261"
     CDS             complement(63..1460)
                     /gene="ccp"
                     /locus_tag="ACHUNN_004261"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0578455.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1865..3514
                     /locus_tag="ACHUNN_004262"
     CDS             1865..3514
                     /locus_tag="ACHUNN_004262"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0578456.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          903 a          927 c          850 g          889 t
ORIGIN      
        1 aaacagactt tattatcgta ataaaaatcg cccaaacatt cctgttcggg cgatcaaatt
       61 aattattgtt tatcctgcat atacggcgtg tacaccccgt tcagactgtg caggaaagct
      121 acgatatcat ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt
      181 ttcaccgccc cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca
      241 acgttacgta aaccgggtac tttctgacgc aatttatcac gctcttcttt agtaacattc
      301 atacgaccaa tatccgccgc cgtaatttcc ccaaagttaa agtctttttt cagccccaac
      361 ggttcaaagg aacgtccacc gagaataata ccaccatgac aagttgcaca tttattatct
      421 ttaaataatt gatagccttt tttctgttgc gccgtcagcg cattttcatc tccacgcaac
      481 catttatcaa atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca
      541 gtaatatttt cgccactgaa accttgcgga tagacgccga ggaactgcgc tttaagctgc
      601 ggatcttttt ccagcttagc aataatttcg tcccaagatt tcgacgccat ttcaatcggg
      661 ttcaacggcg gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc
      721 tcaacgttaa atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca
      781 acaccaatcg atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg
      841 catgaaatgg tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa gcccaacgcc
      901 actttttgcg catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc
      961 tccggagcag tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt
     1021 atttccgccc gctcttcatc actcacctta cccgcccagt gaagcgcggt ataacgcgtt
     1081 ggcggcatag tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg
     1141 ggtttatcag ccagcagtgc cgcacgcacg gcctcgaggt taaaagattt atatccaagc
     1201 ttaatgtcgt aatccatcaa ctgtttcgcg ccaggaatat aataataggc gggtaattct
     1261 gccgaaggcg tgtggcaata gtcgcatcct ttttcgcgga gaaaacctaa aaccttatta
     1321 ttttcactga cagcggatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac
     1381 cacacataac cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta
     1441 atacgtgaga ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa
     1501 attttccaga atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagtgagat
     1561 acgctttttg ataggaatca atcaattaca tgaatataaa ctatcaataa gatagcctgt
     1621 gcctattacc cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt
     1681 ctgaaaatga aaaacaacac gaaatccgta tttttataaa ttccatggat attagtgcgt
     1741 tatatttttt gacaggttaa taaacgtgat ccaccgcacg ctttgtcgcc caccaggcgg
     1801 agcgaatgac tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggttggat
     1861 ccttatgctc aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaagtcga
     1921 tctctgctat gagctggacc cgtatgaatt aaaactggat gagatgatcg aggcagaacc
     1981 ggaacccgag atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta
     2041 tctcgaactg ttcgagcatg ttcagtcggc gaaaattttc ccggacagta aaacctttcc
     2101 cgactgcgca cctaaaatgg acccgctgga tattttaatc cgctaccgta aagtgcgccg
     2161 tcatcgtgat tttgacttgc gcaagtttgt tgaaaatcac ttctggctgc cggaggtcta
     2221 ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg accagctgtg
     2281 gccggtgcta acccgcgaac cacaggatca cattccgtgg tcttctctac tggcgctgcc
     2341 gcagtcatat attgtcccag gcggccgttt tagcgaaacc tactattggg actcctattt
     2401 caccatgctg gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa
     2461 cttcgcctgg atgatcgaaa actatggtca catccccaac ggcaaccgca cctattattt
     2521 gagccgatcg caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt
     2581 acgcggtgcg cgccgctatc tcgaccacct taaaatggaa tatgccttct ggatggacgg
     2641 tgcagaatcg ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc
     2701 gctgctcaac cgttactggg acgatcgcga cacgccgcgt gacgaatcct ggcttgagga
     2761 cgttgaaacc gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc
     2821 gggggcggcc tccggttggg attactcttc ccgttggctg cgtgatactg gtcgtctggc
     2881 gagcattcgt accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga
     2941 gagcgccatc gccaacatct cggcgctgaa aggcgagaaa gagacagaag cgctgttccg
     3001 ccagaaggcc agtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg
     3061 catctaccgc gattacgact ggcgacgcga acaactggcg ctgttttccg ctgccgccat
     3121 tgtgccgctc tatgtcggca tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt
     3181 acgcagccgg ttactgacac ctggcgggat tctggcaagc gagtacgaaa ccggtgaaca
     3241 gtgggataaa cccaacggct gggcaccgtt acaatggatg gcaattcagg gatttaaaat
     3301 gtatggcgat gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaaa cggtgaatca
     3361 gttctatctg gaacagcaca aaatgatcga gaaataccat attgccgatg gtgttccccg
     3421 cgaaggcggc ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt
     3481 acgccgttta attggtttgt acggcgaacc ataatatttt tacagccagc cgctaacttc
     3541 ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZOXR010000295         3543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZOXR010000295 DAZOXR010000000
VERSION     DAZOXR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.302"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUNN_004263"
     CDS             complement(<1..140)
                     /locus_tag="ACHUNN_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0578457.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUNN_004264"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUNN_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0578458.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUNN_004265"
                     /pseudo
     CDS             complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUNN_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(2223..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUNN_004266"
     CDS             complement(2223..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUNN_004266"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR0578459.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          923 a          983 c          783 g          854 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaagaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc ccccccccgt
      901 acggtaatcg agcttctgac cgctgatatc cgccagcatc tgacgccagt actcactacg
      961 cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat gcacgacatc
     1021 catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca gttcattggg
     1081 gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat tgtgtggtgt
     1141 acgctcgccg gaaagataag gcagaaacca aactggctcg acactttcat cagcctgttg
     1201 agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg cccaatccag
     1261 acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta gcgcatggca
     1321 aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag caaaatagac
     1381 ccccgacgtc cccagcgata acattgcctg attagcatca accattccca caccaactgc
     1441 accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac cccacgcttt
     1501 cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg cgggcatctg
     1561 gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct ttgcgacatc
     1621 cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg tcatacgcag
     1681 acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct ccggctcatg
     1741 ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt tgccggtaat
     1801 cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac agcgcccgtc
     1861 gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg ttgctccatg
     1921 catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat cgcccagagc
     1981 tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg accagagtgg
     2041 atgcgggcgc gaaacggtca gcttttccgt ttgcgaatca accacctcac cctgctcgtt
     2101 gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca tatcgatcgt
     2161 tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg cacagttagc
     2221 cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg gcgaccactc
     2281 tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc cagtgacatt
     2341 tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg ctgcgcgatg
     2401 cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc cagtgccatc
     2461 gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact ttgacgacgt
     2521 actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag aatttcatac
     2581 atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca gcccagttgc
     2641 gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc ggtggctatt
     2701 tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt cagtttaatc
     2761 tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc gtaatcatat
     2821 tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgccctg gaagccgatt
     2881 ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg ctcctgacgc
     2941 aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac atagttttca
     3001 ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc ccagctgaag
     3061 acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt aaagcagtta
     3121 gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag gacatcaacc
     3181 atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga aacatccaca
     3241 tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc gacatctgct
     3301 ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt aaacgccccc
     3361 acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc ggcaaaacgc
     3421 aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg acggaatgct
     3481 aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc aaaataggct
     3541 tgc
//
LOCUS       DAZOXR010000296         3528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZOXR010000296 DAZOXR010000000
VERSION     DAZOXR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.303"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1280)
                     /locus_tag="ACHUNN_004267"
     CDS             complement(<1..1280)
                     /locus_tag="ACHUNN_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR0578460.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFK"
     gene            complement(1565..1897)
                     /gene="pemK-mt"
                     /locus_tag="ACHUNN_004268"
     CDS             complement(1565..1897)
                     /gene="pemK-mt"
                     /locus_tag="ACHUNN_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR0578461.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(1899..2156)
                     /gene="pemI"
                     /locus_tag="ACHUNN_004269"
     CDS             complement(1899..2156)
                     /gene="pemI"
                     /locus_tag="ACHUNN_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR0578462.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2249..2902)
                     /locus_tag="ACHUNN_004270"
     CDS             complement(2249..2902)
                     /locus_tag="ACHUNN_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR0578463.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(3000..3140)
                     /locus_tag="ACHUNN_004271"
     CDS             complement(3000..3140)
                     /locus_tag="ACHUNN_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578464.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
BASE COUNT          964 a          822 c          747 g          995 t
ORIGIN      
        1 tgtttaaaat cataatttgg ttgttctgtt cttgagttat taagtagagt tcttatcagt
       61 gtttgccaag catgattgat aaatttattt tctgattctt taaaatatcg tctgattaaa
      121 gcacataaat cttcgacact tttctgacga gcgctagctg cccaacggcc cccttcggtt
      181 tgcaccattg aacgttgtac actgtctcct aactgagcga taaatttctg ttcatcgtca
      241 agttgcatgt tttcatttat gattaattca tacaaagcaa gaaaaacagc ttgatagtaa
      301 cgaggacatt cttgagatga agatgcattg cccagaatgt gtgatctaaa gttcaaatta
      361 tttgcatcaa ataatttttg aatcatctca taaacgaaga ggtaattatt gacaatttta
      421 tctgcaccat ttgtctgaat gtaattctct aattgagttc gtgtatggat tgcatgactc
      481 gtcgaacctt caccgtaaaa tgtatcgagc gatgttgatc ctgatgttgg cttatcatct
      541 aaaaatatat agcccaagat atttgcaact aactcttcat cgtcagagtc tctaatgcta
      601 ggcctactaa tgatatgatt tctaatatag aaatgattgt ctatatttat tccataatcg
      661 agtccatcat tgcaaattga tatgttatgt attttattca gtcccattat atcgctatgt
      721 gtagtatctc ctcttataat tgtcgatatt ttccttacta actctgaaaa attactaacg
      781 caccccgcac ttcgtagttc ttgttttgat aaaattttac cattggaatt tatgcgtcga
      841 aacacttcgt caatgtggtc ctctgttgaa gagtattcag atatagcaaa tctatagcga
      901 gcaattaatg tagaactttc gcgggatagc actggctctt tttgttgaag tttaccactg
      961 tcttttagtt gttttgttaa tgcaatggca tctaaattaa agtatttacc atttaatgaa
     1021 aatcgctgct caataaaact cattaaagct tctagtcttt gcattccatc aaggatttct
     1081 cttttgttgt caatggaaac aaacaatagc aaagggatag ggtattgctg agtcattgat
     1141 gaaatcagtg ctgttttctc ttcgagggac catactaatt ttctttggta tcttcgattt
     1201 ataattaatt tatcatccag ataccactcg tataattctt ctgctgtctt ggagtcaata
     1261 tctaagtttt cattagccat tagatgtatc tcgagtattt agttaagaaa ctgataggtc
     1321 agtatttgca tcaatgttca ttgaatttgt atccgaagtc aatctgatgt aggaacttca
     1381 aaactgcgca aaacacgcaa atacgcacaa gaaagtaggg ctgcgcttgg aattcggaaa
     1441 ataaaccaca tcaaaactac tttttagcca aataacgttt gggaatcacc agaatggtgg
     1501 gacaacagcg gttttagtgc cctaaatcgt acgttttcat ccagttgccc ctcaaacccc
     1561 atgttcaagt cagaatagtg gacaggcggc caagaacttc gttcatgata gtctccggaa
     1621 cccgttcgag tcgttttccg ccccgtgctt tcatatcaat tgtccggggt tgatcgcaac
     1681 gtacaacacc tgtggtacgt atgccaacac catccaacga caccgcaaag ccggcagtgc
     1741 gggcaaaatt gcctccgctg gttacgggca caacaacagg caggcgggtc acgcgattaa
     1801 aggccgccgg tgtgacaatc agcaccggcc gcgttccctg ctgctcatga cctgcggtag
     1861 gatcaagcga gacaagccag atttcccctc tttccatgtc agatttcctc ctgaccagtc
     1921 gccggtgcat ccagccattc tcgttcttca gctgatattt cagcattcgg atcacactgt
     1981 gccagtagct cagccagtga atattgcggg cgtctgtacg gctcaacaat cagccggcca
     2041 ttatcaatga ccatgccaac ttcattatct gtgcccagag acagcgcatt cagcagtgcc
     2101 ggtgggacgg tcagcataac tgagccgcca accctcttca gtcgggtggt atgcattctt
     2161 cacctccata aaagttatat ttaaatataa catccactaa aaaaacacac caggctttaa
     2221 cgcacaatgt ttaataaaaa tataactttc aaccaaacag taaacccagc gtggttgctt
     2281 ccatatgcag caataccggc agcagcaggc caccacttct gatcctggcc actgatgtaa
     2341 tcaaccccac caggaacagt tctgccagtg tcagcaggtt ctgatactgg ctgtgcgcgg
     2401 cgacgaacaa caacgacgtt atcagcgccc ccagccacat cgtccagcag taccgtgaac
     2461 ggaagacgtt cagcataatc ccccggaaca gcgtttcctc attcaacggg gcaaggataa
     2521 agatggtcag caacgtcagg atcacgtcag gtatggactt atcggcaaaa agtttcgtca
     2581 taaatggctc agcaggcaga gccagcgcct taccgagcag aaatacaccg acatacacca
     2641 cggccatcgc accgaccagc cacggtacgc caacgttgcg cagctgacca acgaccggta
     2701 gcggagctat ccaacggcgg tataccagga aaacacacag caggtacatc agaacagtac
     2761 catgactgaa gaacaaatag ttttttcctg atccataaag cagaacggcc tgctccatga
     2821 caaatctggc tccccaacta atgccccagg cagccagcat aaccagcata aactgcagat
     2881 attgattacg tgtttgaatc attgcatcgc ctgtaaattt ttaacttgtc ctatttttgt
     2941 cattaccacg tatatacaca tgtataacaa ttcagatatc gttaccagga tatgccgcat
     3001 cagcggcatg gaaggcggca ctctgttgtt tcatatgata caggagtaaa accgccgaag
     3061 cccggcgtaa gccggtactg attgatagat ttcaccttac ccatccccag ccctgccaga
     3121 ccatacccgc tttcagccat gagagagctt ctgtgcgcgg tcggagtggt cccgacgagg
     3181 gtttacccga agtcggggcg tatctccgcg ttagcgggcc gtgagggccg cttacgagcg
     3241 tgtactgaga acttccagcg agaagactga cagcgatgaa gatgtagtta caacattcat
     3301 aattaaaagc gactctgttc cggccctttg ggccggggcg gggccgcttt tcagttatga
     3361 gggaggggct ttgtggtttc ggttctgcgc tggaccgggg tttttctgga ggttgttttt
     3421 gtgtgttgta actaaagtgg ctccggtcgg ggcccgccgc ttgcggtggg aggtgcatat
     3481 ctgtctgtcc acaggacagg cagtgaatag gttttctttt taaatgaa
//
LOCUS       DAZOXR010000297         3423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZOXR010000297 DAZOXR010000000
VERSION     DAZOXR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.304"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            2..682
                     /locus_tag="ACHUNN_004272"
     CDS             2..682
                     /locus_tag="ACHUNN_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0578465.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            682..1350
                     /locus_tag="ACHUNN_004273"
     CDS             682..1350
                     /locus_tag="ACHUNN_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFR0578466.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            1347..1985
                     /locus_tag="ACHUNN_004274"
     CDS             1347..1985
                     /locus_tag="ACHUNN_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0578467.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYA"
     gene            1978..2232
                     /locus_tag="ACHUNN_004275"
     CDS             1978..2232
                     /locus_tag="ACHUNN_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578468.1"
                     /translation="MPDLKIVELKPSKETDNNNVEVIRLLEEALQHAREGKSQSLALL
                     MINNDGSVLDCWHNGGRPYVMVGAMESLRLDFINANIERR"
     gene            2238..3128
                     /locus_tag="ACHUNN_004276"
     CDS             2238..3128
                     /locus_tag="ACHUNN_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578469.1"
                     /translation="MADIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            3138..3404
                     /locus_tag="ACHUNN_004277"
     CDS             3138..3404
                     /locus_tag="ACHUNN_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578470.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
BASE COUNT          921 a          778 c          918 g          806 t
ORIGIN      
        1 aatggacgta aaaatcacga ttttgcaggt tgatgttgcc aaccttcgcc cgaatacctg
       61 gaacaccaat tcggttggtg cgcagaattt cgaaaaactg aaaggttcta tcgaaaaatt
      121 gggctttttt aagccaattt tggctcgtga acttgaagat ggattttttg aaatcctcgg
      181 cggcgaacat cgctggcgtg ccgctattga gcaaggaatt tcaacggttc cagtgctttc
      241 tgtgggcaaa attagcgatg tcgtagctaa acagatgtca ctggtggaca acgagcgata
      301 cggtgaagac gaccaaatcg cattgcaacg cttcattgaa gaaattcagt cagaacttga
      361 ctatcaactg tctgaaatcg ccccgtatga cgacgaaatc tcgatggttt tagcaaaaga
      421 ggcggcaatc gaccttgaag cactggaggc gttgtctcgt ggtagtgatg agcctgtcga
      481 taccgacaaa cgagagaaaa ccgaacgtgt gggggcggaa catcagacca tgcgcttcaa
      541 agtaactttc gatgcgtcag atcgtgttgc agaaaccata aaaaacatca tcaaagagca
      601 ggctattaac accggtaatg aaatggagaa cgctggtgag gctctggtgt ggctggtcga
      661 ctactacaag gagcgtatgt aatgaccaaa aagtttgaaa tcgtatatcg cgacccggca
      721 gatcttattc cctatgagat gaatgccaaa aagcatgatg aacagcagat cagagatctg
      781 gccgcagcca ttaaaaagcg cggatttgac cagccaatta cggtcgataa gaacgacgta
      841 attattactg gccacggccg tcgtgaggct gcaatttttg ctggacttga gcgtgtaccg
      901 gttattgttc gcgatgatct cagtgatgac gaggttcgtg cgaagcgcct tgaagataac
      961 cgacttgcca gcattgatta cgatgcaatt aagctacaga aagagcttga gtcgcttgtt
     1021 ctggacgata tcgaggtttt cggcttcgaa gagcgtgagt tgaatgttct cgttggcagt
     1081 atgacagaag aaatggacac cgactcgcta gttatcgatc ttggcgaaga aactaaacga
     1141 cagaaggatg aacacaccga gatcagtcgt gaagttgcag cggaagaagt acgtgttgtc
     1201 gacgtattgg gctttaaaac gcttcctgct ggctctgcca ttgttgttgg tgatttgctt
     1261 gcccacatgg aagaaatgac gggagaaagc ggggtagacg catttgtggc atatgcggag
     1321 aaaatctctt ccggggagat ggctgcatga gcaaatacat catcaacgta tcgtttcaga
     1381 cacgcgtaaa taaaaccacg cgcacgttgg aaatcgctga gtcgttcggg cttggcctgg
     1441 acgaaaaaga gtggacgctt tacgacaatc tggagctgga agtgaagcag ggcgatgtgg
     1501 tgtacatcac cggccaatcc ggttccggca aatccgttgt gctgcgcgag ttgcaacgac
     1561 agatgaagga tgaagggctg tctgtagcct ccatcgatga ttttaccttc gataatgagg
     1621 ttaacgtcat cgatcagttg ggcaaaacga ccagtgatgc gcttgggttg ttatctatgg
     1681 ctggtctgaa cgatgcatat ctgtttgttc gcaagccttc tgaaatgtca gacggccaga
     1741 aatatcgtct caagattgcc aaactgattg agtcaggcgc taaagtatgg gctgctgacg
     1801 agttcggcgc tgttctagac cgtgtaaccg ctcaggttgt ggcgtctaac ctccagcgtg
     1861 ccgctcgaaa ggttggtgcg acggtaatgg tggcgacgac tcacgaagac ctgaagaacg
     1921 cgctgcgccc ggatatgcag atcaccaagc actacaaaga acgcgtgaag gtggaatatg
     1981 cctgatttga agatcgtaga gctgaagcca tcgaaagaga ctgataacaa caacgtggaa
     2041 gtcatccgcc tgctggaaga agcacttcag cacgccagag aaggaaaaag ccagagcctg
     2101 gcgctgttga tgatcaacaa cgacggcagt gttcttgatt gctggcataa cggcgggcgc
     2161 ccgtatgtca tggtaggggc gatggagtct cttcgcctgg atttcatcaa tgccaatatc
     2221 gagcgcaggt gatcgacatg gcagatatca tcatcaaacg ctaccgccct gaagagtttc
     2281 cgcgtcatct ggactttctg gagcgaatga ctgtcacaaa gggaactgta gaggactggc
     2341 acgctcttaa gtcgcttcac tacaaaacag acggcaaacc tttcgcgcca acttactatc
     2401 gctgcgaact tgatgaccgt ctggtgggcg tcgtggttat ggcttacccg aaactactgt
     2461 tggcacctcg ccaccgcatg tttcctaagt tgaaaccaac cactaatacc accgtagcta
     2521 accagtactg gggtcggtac gtgaataaca actttgcggt gatcagtcgc tcagttgtgg
     2581 atactcagta tcgtggcgta ggcgtctctt atcgaatgat taacctggtt agcaggatgc
     2641 atgaccggcc aatcattgag atccagtcct cgatgagcaa atacaatccc ttcgccatga
     2701 aagcagggtt taagttcatc cgccctgagc gaccgaagag ctatgaaagt gcactgcgtg
     2761 tattccagcg ccatttccgt tccgaccctg gtgataacga ggcgatcgtc aaagagttgt
     2821 tcgcaatgag cgagtctcgt cgtcgccgtg cactgcgtga tctggtggcg gactaccaca
     2881 agaacagttc cctggcaaaa gctgggcgga atcgtggcac gacgattcag gacattgccg
     2941 acagtctggt ggacgaggcc agcattgtga agctgctcaa ggacattcac aacctgagct
     3001 ttacgtctcc gttgtatggt gtgtaccgaa accctgactt tggtcgtcga ctgcctgaca
     3061 cgctgccact gctggcattc gacaaacaac ctttggataa accgttagaa attgctttac
     3121 cggcataagg atttgccatg acgttaaccg acaaacaaaa ggacatcatc aaaacgctca
     3181 atctcggtta tgagcgaggt catctacttg atctggacga attgcttgaa gttttgccgt
     3241 acaagacaac caagcaaagc atccagttct caattcgcgc tctgataaaa aaggggctgg
     3301 tggagaaagg acatacgcgc caacgcagtg acaatcgcta tcaccgccgg actcttgggt
     3361 taaccacttt aggtcgagcc aaagcgaagt tactggtgat gtaatcggtc tgggagctta
     3421 ttt
//
LOCUS       DAZOXR010000298         3423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZOXR010000298 DAZOXR010000000
VERSION     DAZOXR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.305"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(205..840)
                     /gene="nth"
                     /locus_tag="ACHUNN_004278"
     CDS             complement(205..840)
                     /gene="nth"
                     /locus_tag="ACHUNN_004278"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0578471.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(840..1535)
                     /gene="rsxE"
                     /locus_tag="ACHUNN_004279"
     CDS             complement(840..1535)
                     /gene="rsxE"
                     /locus_tag="ACHUNN_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0578472.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(1539..2159)
                     /gene="rsxG"
                     /locus_tag="ACHUNN_004280"
     CDS             complement(1539..2159)
                     /gene="rsxG"
                     /locus_tag="ACHUNN_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0578473.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(2163..3221)
                     /gene="rsxD"
                     /locus_tag="ACHUNN_004281"
     CDS             complement(2163..3221)
                     /gene="rsxD"
                     /locus_tag="ACHUNN_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0578474.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(3222..>3423)
                     /locus_tag="ACHUNN_004282"
     CDS             complement(3222..>3423)
                     /locus_tag="ACHUNN_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578475.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAA
                     VAAAIARAQAKKAAQQKVVNED"
BASE COUNT          851 a          898 c          862 g          812 t
ORIGIN      
        1 ttacattgcg acagcatcgt gatcttgatc acgaatgaat agaaaatttc gctattaaaa
       61 aaacaattaa cctgcatttt tggttatccg caaacggatt acctgatgat aacgctaagc
      121 aatggcatca ttcacgttaa aaagatgttt taaaagagga taaagaaagg ttatcaatgg
      181 ggtaatcggt gttacccctt ttcttcagat gtcaactttc tctttgtatt cacaaagatc
      241 ttcaataata caagagccac agcggggctt gcgggcaatg caggtataac gcccgtgcag
      301 gatcaaccaa tggtggcagt cgactttaaa ctctgccgga accactttca gtagcttttc
      361 ttctacctgt tcgacgtttt tccccggcgc aaattgagta cgattacaaa cgcggaaaat
      421 gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg tttaatacga cgttggctgt
      481 tttacgacct acgccgggca gggcttcaag cgcagcacga tcttccggaa cctcgccatt
      541 atgctgctcc agtaagatac ggcaggtttt gatgatattt tctgctttgc tgttataaag
      601 cccaatcgtt ttgatatagg ttttaacccc ttcaacgccc agttcaagca tcgctgcagg
      661 cgtattcgcc accgggtaaa gtttcgccgt cgccttatta acactgacat cggtcgcctg
      721 agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt
      781 gggatgagga ttgttctcac gcaggcgagt gaggatctcc aggcgttttg ctttgttcat
      841 cagacattcc ctgtttcacc gtttggcaat gcacgttcgg cagctgcttc agtacggcgc
      901 tttttcattc tttcatcaat caggtatttt cctgccagca tcagtcccag gccaataaat
      961 gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg tgtggaaaat ctccacgcgt
     1021 aatacttttg cccagctacc taacagcgca tctgcaccgt caaacaatgt gccattgccg
     1081 ataatttcgc gtagtgaacc cagcacgaac atggcgcagg ttgcgcccat accaattgaa
     1141 aagccgtcca gtgccgaaag cgccggacct tttttggcgg cgaaggcttc agcgcggccc
     1201 acaacgatac agttagtgac aatcagcgga ataaaaatcc ctaatgattg atacaggcca
     1261 aaggcgtagg cgttgatcag catctgtaca gcgctgacca ccgaggcgat gatcatcacg
     1321 taaatgggaa tgcggatctc ggctggcgtc cagtgacgca gcgtcgaaat ggtcaggttg
     1381 gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca gagcgttagt ggcagtggac
     1441 gtgaccgcca acagaggaca aaggccgagc aactggacca gcgcagagtt gtttttccac
     1501 aacccctgaa caataacgtc tttaatttcg ctcacgcttt attctccaca ggcaggaagt
     1561 tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg cgcgttttac cgcattaacc
     1621 accgcgcggg gagtaatcgt cgcgccggtg aactggtcga aatcaccacc atctttcttc
     1681 accgcccagt gcgcatcatc cgcaccactg atttttttac ccgcaaaatg ggtgatccag
     1741 tcagaaaggc gcagttcgat tttatcgcca agccctggcg tttcgtggtg ctctgtcacg
     1801 cgcgtgccaa gtaccgtgcc gttaaaatcg gctcccacca gcagctgaat cgcaccggaa
     1861 tagccatctg gcgcggttgc ttccagaacg gcggctaccg gtttgtcatc ctgtttggcg
     1921 atgtaaaccc gatgctcacc tttacctaac tctggcgcag ttaccagata gcaactctgt
     1981 gccagcgtat tgttatagcg ttcggctggc agcacctgat caaataatgc cttttgttgc
     2041 agactggcct gttcagcaat cgtcgttttg gtcatctggt tgatggccgc agttaaccct
     2101 gttgaacccg ctgcaaatag cgctaacgta atgccgtgtt ttcggatagt tttcagcatg
     2161 gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt agtaatcgat cagaggaacc
     2221 gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg
     2281 atcaaccaga ctaataagcc cgcaagcgcg ccgaaaataa gacgaccacg attggtcgta
     2341 gaagcggtaa ccgggtcagt caaaataaag aatgcgccga gcatggtcgc accagacagc
     2401 agatgaattt gcggtgctgc cagtgtttct ggtgagaaca accagcccaa cgttgcgcat
     2461 aacgccagcg ttactaagaa gctgagggga atatgccagc gaatcgcttt ctgccatagc
     2521 aaccacaggc cgccagccag ccaggcgaga tttacccatt gccaaccagc gcccgccaga
     2581 ataccgctgt agatcggata ttgcataatc tgttcaaccg aatgaccggc acggacagag
     2641 gttttaaatg tatccagcgg tgtcgcctga ctaatgccat caatacctaa gcgtagtgtg
     2701 ttcatatcac caccactggc ggtatgcccg ctgaaaataa cctgtatggc gtcgataaaa
     2761 ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc tggtcatctg caccgggaag
     2821 gagatcagta agaccacata accaatcatt gccggattaa acgggttttg ccccagaccg
     2881 ccatacaact gtttagcgat gatcaccgca aacaccgtac ccagcacgac catccaccat
     2941 ggcgcgaggg ggggaatact taccgccagc aataagcctg tcagcaatgc tgagttatct
     3001 ttcaacgttg cggctaccga ctgcttgcgt agtttgagta cgagagcttc ggctaacaga
     3061 gtactaaccg atgccaacag gatctgaacg agagtacccc aaccaaaaaa ccacagttgc
     3121 gctgcgattc ctggcacggc tgcgagcaac accaacagca taatgcgcga tgtctggcgc
     3181 tggttatggg tataagggga gctagctatt ctgaatacca tttagtcctc gtttacaacc
     3241 ttctgctggg cggctttttt ggcctgagcg cgggcaatag ccgcggcaac tgccgctttg
     3301 cgcggatcaa cctgttcttc tggttctgcg ttagtctgtt gctgttccag cttgcgcgct
     3361 ttggcacggg caatagcggc ttcgacggcg gctttgcgcg gatcaacctg ttcttctggt
     3421 tcc
//
LOCUS       DAZOXR010000299         3346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZOXR010000299 DAZOXR010000000
VERSION     DAZOXR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.306"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            149..577
                     /gene="uspC"
                     /locus_tag="ACHUNN_004283"
     CDS             149..577
                     /gene="uspC"
                     /locus_tag="ACHUNN_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR0578476.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(584..2008)
                     /gene="otsA"
                     /locus_tag="ACHUNN_004284"
     CDS             complement(584..2008)
                     /gene="otsA"
                     /locus_tag="ACHUNN_004284"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR0578477.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1983..2783)
                     /gene="otsB"
                     /locus_tag="ACHUNN_004285"
     CDS             complement(1983..2783)
                     /gene="otsB"
                     /locus_tag="ACHUNN_004285"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0578478.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2950..>3346)
                     /gene="araH"
                     /locus_tag="ACHUNN_004286"
     CDS             complement(2950..>3346)
                     /gene="araH"
                     /locus_tag="ACHUNN_004286"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0578479.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT          845 a          836 c          792 g          873 t
ORIGIN      
        1 ttatatgatc accgggagtg attgattaat tgtctttact aataatcaga ctaatattta
       61 cctgtttgac cgagttggga ttgcgtcgtt tctccattag gagtaaagct ttaatgtcac
      121 ctgaagttca cagaataagg aacaggttat gagctatagc aatattcttg tcgctgttgc
      181 ggttacaccg gaaagtcagc aactgctggc aaaagcggta tctatcgcca ggccagtaaa
      241 gggacacatc agtttaatta ctctcgcttc cgacccggaa atgtacaatc aattagccgc
      301 gccgatgctt gaagatttac gcagcgtgat gcaagaagaa acgcaaagct ttcttgataa
      361 gttcattcag gatgcggggt atcccgttga caagacgttt attgcctacg gtgagttaag
      421 cgaacatatt ctggaagtat gtcacaaaca tcatttcgat ttggtgattt gcggtaatca
      481 caatcacagt ttcttttcgc gagcatcctg ctcggcgaaa agagtgattg cctcaagtga
      541 ggtcgatgtg ctgttagttc cacttacggg agattaaccg ctcctacgca agctttggaa
      601 aggtagcaac tttatcgcgc tgctggcttt ccgcgcttcg cggaactatc tgctttaggt
      661 cgctaatgaa gcactcctgc cagtggttaa tatcgttttt cacgataacg tccagcattt
      721 ctgcatgacg ggaaatacgt tccgccagcg acatagtcaa tgcacgatcc agcgcagctg
      781 caacttcgtc acgatcgtag gggttaacaa ttaacgccga cgttaactcg tttgccgctc
      841 ccgcaaattg cgaaagaaca agaacgcccg ggttggctgg gtcctgagca gcaacatact
      901 cttttgctac caggttcatc ccgtcacgca gtggcgtcac taagcccacg tcagagtagc
      961 ggaatatttt catcagtaat ttacggtcaa aatgctgatt caaataataa agcggcgtcc
     1021 agcctaattg cccgtattta ccattaattc gtccagcttc attttcgagc tgatgacgaa
     1081 tatcctgata ggcttgcaca tcaccacgcg acgttggtgc aatctgggta taacgaattt
     1141 taccatgatg ctgcggatat ttttccagca acgcttcata ggcgagaaaa cgctctggca
     1201 aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt ttcagttccg
     1261 ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct atttctttcg
     1321 gttcaatgcc gatcgggtag acttctgttc gaaatgcttt gccccaggct gtatggcttt
     1381 ttgcgctacg tgtcgtgacg cgggtcaggt tagaaagaca atccaggaac gccagacgat
     1441 cgttttctgt ctggaaaccc agcaaatcat aatcacaaag ctgttcaagc aaggtgtcat
     1501 atgtcggcag cgcgttgaag atttccggtg tcgggaaagg aatatgcaga aagaaaccaa
     1561 tgcgattatt cactccccgt ttgcgtaatt catgcgcaaa tggcaacagg tgataatcgt
     1621 ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc agcaacgcat
     1681 ttacgcgtag atagccgtcc caggcaggac gctgaaattg caccagatcg agccgataat
     1741 gaaaagcggg ccagagaacg gcattggaga attggttgta gtattcgtca aggtcctgtt
     1801 cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt cacctttttt agcggctgat
     1861 cctcattccc tgtttcacca ctccagccaa accacagtcc gcctgcggct ttcagtgccc
     1921 ccagtatgcc aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc
     1981 ggttagatac tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt
     2041 tgtaatgcgg tggttatcat ttcaagccag ctccagacat ccggcacacc cgccagtcgc
     2101 catgatgcct gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg
     2161 actgcgaagc cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata
     2221 aagggagctt cctgcataaa agctgcaatt gcctcacctt tactggtacc tctcggtttg
     2281 atctcgacaa cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt
     2341 tgcgctaatg tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa
     2401 gccatccctt tcgcctccag ctccgcgccg ggatactgag cgatgactgt atgcagttgc
     2461 acgctaatat cacgcgcaat cgcatccggc agatgaacga tatgtgtttt accattgatg
     2521 tcacggcgct ccgccccatg cacgcccgct aacgggaagc gataaggttt tgccagtgcg
     2581 tcaagctcca ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc
     2641 agtagctgta gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg
     2701 atttccgcca gcgttccatc aagatcaaaa aaccaggcat atttcgcgga tagttcaggg
     2761 gtttcggtta acggttctgt cacccggctc tcctccttct cttttcttat tgttttgcgc
     2821 agaacgcgct ttacagacat gtaagtatag acagtgtgac gggggtcgcc atttgcaatg
     2881 aagaacagcc aaccgttgtg gcggctggct gtggtgggaa aaaacgttaa attgttgtgg
     2941 aaaaaagcat cagacaatgc gtttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc
     3001 cagcaggatt aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat
     3061 ggcgttttcc acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat
     3121 gccacctttc agagaaacgc cacctaaaac gcaggcggag ataacaataa gctcataacc
     3181 aatcgacgtc attggctgcc cactagtcat acgtgaagcc agaataattc cggctatcgc
     3241 agataccagg ccagagagaa caaagataat aattttggtg cgaacaaccg gtacacccgc
     3301 cagacgcgca gcctcttcgt tcccgccaat cgccagggtg ttacga
//
LOCUS       DAZOXR010000300         3336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZOXR010000300 DAZOXR010000000
VERSION     DAZOXR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.307"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUNN_004287"
     CDS             complement(<1..312)
                     /locus_tag="ACHUNN_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR0578480.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUNN_004288"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUNN_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0578481.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUNN_004289"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUNN_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0578482.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          903 a          870 c          726 g          837 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaa
//
LOCUS       DAZOXR010000301         3294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZOXR010000301 DAZOXR010000000
VERSION     DAZOXR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.308"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHUNN_004290"
     CDS             complement(89..652)
                     /locus_tag="ACHUNN_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0578483.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHUNN_004291"
     CDS             complement(756..974)
                     /locus_tag="ACHUNN_004291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578484.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHUNN_004292"
     CDS             complement(1133..1519)
                     /locus_tag="ACHUNN_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0578485.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHUNN_004293"
     CDS             complement(1509..1880)
                     /locus_tag="ACHUNN_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0578486.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHUNN_004294"
     CDS             complement(1880..2137)
                     /locus_tag="ACHUNN_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578487.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..>3294)
                     /locus_tag="ACHUNN_004295"
     CDS             complement(2134..>3294)
                     /locus_tag="ACHUNN_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0578488.1"
                     /translation="ITDTGRKTWVKSSLEHYVAALRRNAALRDAEKTLNEALQKLRDA
                     HTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEVVERVECRNQGLEKSRTLMT
                     GIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKVTEQGHGVLLFTMEMANIQI
                     GERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTGRNIWMVDQANLTIDEICAT
                     TKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISKGLKGLAKSGGFPLIALSQL
                     SRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVYNPDTQATGIAEINITKQRN
                     GSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYSNTRTDSSKMERFF"
BASE COUNT          766 a          915 c          728 g          885 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taat
//
LOCUS       DAZOXR010000302         3232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZOXR010000302 DAZOXR010000000
VERSION     DAZOXR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.309"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            118..834
                     /gene="nanC"
                     /locus_tag="ACHUNN_004296"
     CDS             118..834
                     /gene="nanC"
                     /locus_tag="ACHUNN_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0578489.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            854..1960
                     /gene="nanM"
                     /locus_tag="ACHUNN_004297"
     CDS             854..1960
                     /gene="nanM"
                     /locus_tag="ACHUNN_004297"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0578490.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2025..3005
                     /locus_tag="ACHUNN_004298"
     CDS             2025..3005
                     /locus_tag="ACHUNN_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0578491.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3115..3232
                     /locus_tag="ACHUNN_004299"
                     /pseudo
     CDS             3115..3232
                     /locus_tag="ACHUNN_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          952 a          688 c          760 g          832 t
ORIGIN      
        1 taagctaccc gttgccaaag acactataag cctggcaaaa aatattacac aacataaatg
       61 ctaattgttt atgcgggctt tgtattgctt tctgtatcct acaaatgagt gaaatttatg
      121 aaaaaggcta aaatactttc tggcgtatta ttactgtgct tttcgtcacc attaatttct
      181 caggctgcga cactggacgt acgtggtgga tatcgtagcg gaagccatgc ctatgagact
      241 cgactcaaag tcagtgaggg atggcaaaat ggatggtggg caagcatgga aagtaatacc
      301 tggaatacca ttcatgataa taaaaaggaa aatgccgcac tcaatgatgt tcaggttgaa
      361 gttaattacg cgattaaact tgatgatcaa tggacggtgc gcccgggaat gttaacgcat
      421 tttagcagca acggcacacg ctacggaccc tacgtaaaac tgtcctggga cgcgacaaaa
      481 gatcttaatt ttggcattcg ctatcgttac gactggaaag cttaccgaca acaagactta
      541 tccggtgata tgtctcgtga taacgttcat cgttgggatg gatatgtcac ttaccatatt
      601 aatagtgatt tcaccttcgc atggcaaacg acgctataca gcaaacagaa tgattatcgc
      661 tatgcaaacc acaagaaatg ggcgacggaa aatgcctttg ttctacaata ccatatgacg
      721 cccgatatta cgccatacat agaatatgac taccttgacc gccagggtgt ttacaacggc
      781 agagataatt tatcggaaaa cagttatcgc attggtgtgt catttaaact gtagtagaca
      841 ggagacagtc acaatgaata aaactataat ggcgcttgct atcatgatgg cttcatttgc
      901 cgcaaacgcg tctgtattac cggaaactcc tgtgccattt aaaagtggta ccggagcaat
      961 tgataacgac actgtctaca ttggtttagg tagcgcaggt acagcatggt acaagctaga
     1021 tacacaggcc aaagataaaa aatggactgc gttagctgca ttccctggtg gaccgagaga
     1081 tcaagcaacc tctgcattta ttgatggcaa tctatatgtg tttggcggca ttggcaaaaa
     1141 cagcgaggga ttgactcagg tatttaatga cgtacacaaa tacaacccca aaaccaatag
     1201 ctgggttaaa ttgatgtcgc tcgcgccgat gggcatggcg ggtcatgtga cttttgtaca
     1261 caacggcaag gcttatgtta ctggcggtgt taaccagaat atcttcaatg gctattttga
     1321 agatctcaac gaggctggaa aagattcaac cgctatagat aaaatcaacg cccactattt
     1381 tgacaaaaaa gcagaagatt atttcttcaa taagtttctg ttgtcttttg atccctcaac
     1441 acagcaatgg agttacgctg gcgaatcgcc ctggtacgga acggctggtg cggcggttgt
     1501 gaataaaggt gataaaacct ggcttattaa tggcgaagcc aaaccaggat tgcgaacgga
     1561 tgccgtattt gaacttgatt tcaccggtaa taatttaaaa tggaataagc ttgatcccgt
     1621 ctcatcacca gatggcgtcg ctggcggttt tgcggggata agcaatgatt ctcttatatt
     1681 tgccggaggg gccggattca aaggttcacg agaaaattac cagaacggta agaactatgc
     1741 gcatgaaggc ctgaaaaaat catatagcac tgatattcat ctttggcata acgggaaatg
     1801 ggataaatcg ggtgaattat cgcaaggtcg ggcctacgga gtatcattgc cctggaataa
     1861 tagtttatta attattggcg gtgaaactgc aggcggcaaa gcggtgacgg attcagtttt
     1921 aatttctgtg aaggataata aagtcacagt acaaaactaa cgcttcaggg ccccggtaaa
     1981 ggggccctgc tatcaatttg tcatttgtat tacgcgaaaa ttatatgaac gcaataatat
     2041 cgcccgatta ttactatgtt cttaccgttg ctggtcagtc taatgccatg gcgtatggcg
     2101 aaggactgcc attaccggac agggaagatg cgcctcattc cagaattaaa caattagcga
     2161 gatttgcgca tacgcatccc ggaggcccgc catgtcactt taacgacatt attccactga
     2221 ctcactgccc acacgatgtt caggatatgc agggttatca ccatcctctg gcaacgaatc
     2281 atcaaacaca gtacggcacc gttggccagg cactgcatat tgcacggaaa ttactgccct
     2341 tcattcctaa taatgcaggg attcttatcg ttccgtgttg ccgtggcgga tcggctttta
     2401 ccgcgggcag cgaagggaca tattcagaac ggcacggagc cagccatgat gcttgtcgtt
     2461 ggggaacgga tactccgctg taccaggatt tagtcagcag aacgcgagcc gcactggcaa
     2521 aaaatccgca gaacaaattc ctcggcgtat gctggatgca aggcgaattt gacttaatga
     2581 ccagtgacta cgcgtcacac cctcaacact ttaatcatat ggttgaagcc tttcgtaggg
     2641 atctaaaaca ataccattct cagcttaata atattactga cgcgccatgg ttttgcggcg
     2701 ataccacctg gtactggaaa gaaaatttcc cgcattcgta tgaagctatt tatggcaatt
     2761 atcaaaataa tgttttagcc aatattattt tcgtcgactt ccagcaacaa ggtgaaagag
     2821 gactgacgaa cgcgcctgat gaagacccgg acgatttaag cacgggatat tacggttcag
     2881 cgtaccggtc accggagaac tggacgacgg cactgcgaag cagtcatttc agcacggcag
     2941 cccgtcgggg gattatttct gacagatttg tagaagcaat tttgcagttt tggcgcgaaa
     3001 ggtgagcggg gagtttattc atgggggatg tccttgttcg ttctcagagt agacaacaaa
     3061 aaacaaataa gatcaaggta atatctcttc acttgcgggc tcgaacagca ggcaattgcc
     3121 aatataataa tctggcacaa gggtgaaaaa cgcgcaccgc ataaaccgtt gttattgctt
     3181 acagattagc cggatgcctt aatagacatc cgcgcctttt cgattatggc tc
//
LOCUS       DAZOXR010000303         3231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZOXR010000303 DAZOXR010000000
VERSION     DAZOXR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.310"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHUNN_004300"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHUNN_004300"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0578492.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHUNN_004301"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHUNN_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0578493.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHUNN_004302"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHUNN_004302"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0578494.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHUNN_004303"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHUNN_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0578495.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          758 a          864 c          773 g          836 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt a
//
LOCUS       DAZOXR010000304         3202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZOXR010000304 DAZOXR010000000
VERSION     DAZOXR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.311"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            20..1579
                     /locus_tag="ACHUNN_004304"
     CDS             20..1579
                     /locus_tag="ACHUNN_004304"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0578496.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVEAGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            1582..1860
                     /locus_tag="ACHUNN_004305"
     CDS             1582..1860
                     /locus_tag="ACHUNN_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578497.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQMKLKEKNIKMSKAEVINTI
                     LETMTMAEFDKVSSAVGVSAKAREKIMRIYENSNMTKEDLEQILSKLP"
     gene            2042..2635
                     /locus_tag="ACHUNN_004306"
     CDS             2042..2635
                     /locus_tag="ACHUNN_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578498.1"
                     /translation="MKRSLLLACIYLSSFSANALSDIVSDMQKTYSMYTSEDVLKYES
                     RGLIQPVWFRFSDNAEETILNAGKERLRLQINGTAVPENVNVSSDEAMKPSGIVLEVQ
                     DGTLYCDEMATTGCQVNIRVGNEEPIVTYAKAEGNTSGKVLFIDENTSKRIVRDIQKT
                     ANSKSVDKKIYVEVPFFVVGNKEFKFGLLKTPLRVSE"
     gene            2632..3153
                     /locus_tag="ACHUNN_004307"
     CDS             2632..3153
                     /locus_tag="ACHUNN_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0578499.1"
                     /translation="MRTLDITYGIPAEVWPRDYEHAERTLLFWRSAKFPVRVTLEDGQ
                     VFCMYVYGLLSARNKLNLTPDPLSNTNRIRLPLERISTIESGIEEVDTSFTGRLTLNN
                     EQMVNQPSRRDFFKICRQAYQNGKSIRVYMADGREIEGTSDGVDACLVTLKLEDGRKI
                     LIFFDWVERILPF"
BASE COUNT          969 a          669 c          744 g          820 t
ORIGIN      
        1 gcactaagga aagaggttta tgactgttcg gattgtatct aacgcagtaa atgcgcttat
       61 ttctggcgca gatgacaagg taaagcaact ggtgcagcaa atgttgagct acgaagtcga
      121 ggctggcgac tggaagggca caagcacaat gttcaactgg agcaaaaact cgttccctgc
      181 tggctttgcc aagcccgtgg cggctaattt gaacaaggcg ggcatcaaat gtgttcatat
      241 ccgcaaagac aaagccccgg cgcttggtaa accaaatccg gcggttaacc cattcccata
      301 caatcctgat tatgcgtatc aggatcagac tgtggaaaca ctggttcgag agggaatgat
      361 gattgcgcag atcgctactg gtggcgggaa atctaacgtt gcctgcaaag cagctgcacg
      421 tatcggtcga atgacattat ttttaacaac ccgctctgtt ctgatgtttc aaatggccga
      481 aaacttccag agatccatcg actaccgcgc caaaaatggc gaaccgtggt taaaagacca
      541 aaaggttgga gtcattggct cgggtgagtt ccaggtatca cgacatatca acgtcgctac
      601 agttcaaact cttgcaagtt tcctcgaaga accaccacgc gatgcaacac cagataagaa
      661 aagctaccac ctcaaacgtc gggagttggt gaaacgcttc ctttcaagtg tctctcttct
      721 tattctggaa gaggcgcatg agtcttcagg ctcaaatttc tatgacatcg ccagattatg
      781 tgtgaacgca gactatcgtc tggcgcttac agccacgccg ttcatgaagg attcgacgga
      841 agccaacatg cgcctgatgg cggtggccgg gcgaattgaa attaaagtca cagaaaagta
      901 tctgattgat cgaggcattc tggcaaagcc gtacttcctt tatcataaag ttgcctacaa
      961 gccagacgag gccagaatca aggccgaact tgccaacaaa cacctcaatt ttagagttgg
     1021 tatgagcacc gcctaccaaa aggcttatca gttggggatc gtgtataatt tgggacgtaa
     1081 cgaggccatt gtgcgcgaag cattgctcta taagcaacat tctctcaatt gtatgactct
     1141 ggttcgcctt aaacgccacg ggcaaatcct gatggaaatg atgaaagagt ccggccttag
     1201 agttgacttc atctatgggg aatctaacca ggcgacaagg caagcaaagc tgaacagttt
     1261 agcgtctgga gaaatagatg ttttaatagg ctcgactatt ctggatgtcg gtgttgatgt
     1321 gccaagcgtt ggtgcggtca ttcttgctgg tggtgggaaa gcagaagttg aaatgaggca
     1381 gcgtgtcggt cgtggcttac gagccaagaa gaatcaggca aacgtgtgtt ttatcactga
     1441 tttcattgac attagcaaca aatacctgtt gtctcactct tatgagcgaa aacacatcat
     1501 cgacaccaca cctggctttg cagaaggtgt attgcctatt gatggcgcat tcgattttgg
     1561 agttctgaaa cgagattagt tatgagcgaa aagaaaacaa cttattgtca ggtggcattg
     1621 tccgataagg ccaatgacaa acttggaaag tttcaaatga aactaaaaga aaaaaacatc
     1681 aaaatgtcta aggctgaagt tatcaatacc attctggaga cgatgacgat ggctgaattt
     1741 gacaaagttt catctgccgt tggagtctct gctaaggctc gtgagaaaat catgcgcatc
     1801 tatgagaact ctaatatgac aaaggaagat ttagagcaaa ttctaagcaa actgccgtaa
     1861 tattggattc ttagtcaatt tcagaattgc cagacttgaa gagctaatag acagagtgcg
     1921 gcaacaacgc ctttgccgca cagcttttag agaaaacacg tctgctattt acagttgaaa
     1981 acttatcgtt tgagactatt ggatatttat aattctactt tgcaataatc gctgtataaa
     2041 aatgaaacgt tcgttacttc ttgcttgtat atacctctcg tcattttccg ccaatgcgtt
     2101 gtcagatatc gtctccgaca tgcaaaaaac gtacagcatg tatacttctg aagacgtact
     2161 aaaatacgaa tcacgaggtc tgattcaacc agtctggttc agattctcag ataacgcaga
     2221 agaaacaata cttaatgcag gaaaagaaag attacggttg caaattaacg gaaccgctgt
     2281 accagaaaac gttaatgtaa gctctgacga agccatgaag ccatctggaa ttgttttgga
     2341 agttcaagac ggcacacttt actgtgatga aatggctaca acaggttgcc aagtaaacat
     2401 tagggttggc aatgaagagc ctatcgttac ttacgccaag gctgaaggga atacaagtgg
     2461 aaaggtattg tttatcgatg aaaatacctc aaaacgtatc gttcgtgaca tacagaaaac
     2521 agcgaactct aaatccgtcg ataaaaaaat ctatgttgag gttcccttct ttgtcgttgg
     2581 taacaaagaa tttaaatttg ggttgctgaa aacacctcta agagtatcag aatgagaaca
     2641 ttggacatca cctacggcat accggctgaa gtatggccgc gagactacga acatgctgaa
     2701 cgtaccttgc tcttctggcg gagtgcaaaa tttcctgtaa gagtcactct cgaagacggc
     2761 caagtgtttt gcatgtatgt ctacgggcta ttgagcgcaa gaaataagct gaacctcaca
     2821 ccagatccgc ttagcaacac aaatcgtata aggctccctc ttgagcgcat tagcacaatt
     2881 gagtcgggca tagaagaggt tgacacttca ttcacaggtc gactaacgct aaacaatgaa
     2941 cagatggtta atcagccatc acgtcgtgac ttttttaaga tatgtcgtca ggcttaccaa
     3001 aacggcaaat ctataagggt ctacatggca gacggtcgcg aaattgaggg aacgtcagat
     3061 ggcgttgacg cctgtctagt cacacttaag ttggaagacg gtagaaaaat acttattttc
     3121 ttcgattggg ttgaacgaat ccttccgttt taatttctat aagggtaata accggctaag
     3181 tccggttatt attttacatg cc
//
LOCUS       DAZOXR010000305         3193 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZOXR010000305 DAZOXR010000000
VERSION     DAZOXR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.312"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHUNN_004308"
     CDS             <1..358
                     /locus_tag="ACHUNN_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578500.1"
                     /translation="FLSMNESDLEVSSKKTSKQDREITDVSVDSDHKNTQCPATDDEL
                     DPFDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDDYYLSPKKKQTQDDK
                     KQKIKPKMYFAGGGIK"
     gene            358..648
                     /locus_tag="ACHUNN_004309"
     CDS             358..648
                     /locus_tag="ACHUNN_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578501.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            969..1259
                     /locus_tag="ACHUNN_004310"
     CDS             969..1259
                     /locus_tag="ACHUNN_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578502.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1259..1678
                     /locus_tag="ACHUNN_004311"
     CDS             1259..1678
                     /locus_tag="ACHUNN_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578503.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            1846..2169
                     /locus_tag="ACHUNN_004312"
     CDS             1846..2169
                     /locus_tag="ACHUNN_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578504.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2599..>3193
                     /locus_tag="ACHUNN_004313"
     CDS             2599..>3193
                     /locus_tag="ACHUNN_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578505.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1060 a          573 c          667 g          893 t
ORIGIN      
        1 ttttctctct atgaatgaat ctgaccttga agtaagctct aaaaagactt ctaaacaaga
       61 cagagaaatc actgatgtga gtgttgatag tgaccataaa aacacacagt gccccgccac
      121 tgatgatgag ttggacccct tcgatgatga agaggatgaa cgtgaaaaag ccattaagag
      181 ggcagagaag aaaaagagat tcgagcttgc aagacaaaag aacaaagtat cgggtttaga
      241 cccattcgat gacagtgaag atgattatta tctttcaccg aaaaagaaac aaactcagga
      301 tgacaagaag caaaaaatta aaccgaagat gtattttgcc ggtggaggaa ttaagtaatg
      361 aaagaatatc acggtgaaaa gagatacaaa gattacctgt taaagcgtta cagcatttct
      421 cgtgaaggtc atcttatgaa ggatactcac ggggaagttt accggattag accaaagaaa
      481 gaagggaggg gttatttctt ttacgatggt gtgacagatt taaagattga cgctttgagg
      541 tttgcagtgc tgtatcattt tgatatctgg aattcaatcc accagctacg attaaaagac
      601 ggtgacccca gcaacttaaa ggccaccaat attatcacaa aacgttagtt tgttttacca
      661 tcatcaatcg taattgtgat ttcatatttg aaaagcttaa tgctgaaatt gaatcgtgta
      721 cttctggcga taatcatttt taaaagctga tacacaagaa gcaccagaaa cacaaagcaa
      781 cctaagcttg cgaatctgtg agattcagca attgtttcaa gaaagcccat taattcccct
      841 tatttatcaa agggaatgac ctgtatcagc cccctacaac catattaaca tgtaaatgag
      901 ttgttgacaa tatgttataa atatgttaaa gttagttata agtcaaagat tttaagaagg
      961 aaaatttaat gagacacaca gttacacgcc ttttgccaga ggcatattcc agactggcag
     1021 caatcaaaga caaataccca gccatgagcc acacagcact tgttggtgag attttgaagg
     1081 aacacatagc ccttacaccg tttgacgcta tcaagggcgt tgcagagggt tctaaggcca
     1141 tcagaatcac aacgggccaa ctggaactac tcaaagcaca gtccgcagtg gcaggttgct
     1201 caatgattca ggtgttgagt gccaaggttc atcaactggc ggataagctg ggggtttaat
     1261 gctatgcaca tttgattatc atgcgtttta tcgccataga ggtcaccttg tccgtgagaa
     1321 taacggaaca tggaaagatt taactcgtaa tgctgaaccc ttcacacacc agcaattaaa
     1381 atcccgcttc tccagatgga agaatgcacc agcagatact ggtgattttg agattatcca
     1441 taccttcgat tatgcctcag aagatgacct tcacgatgca attaagctga ttgaagactt
     1501 caatacctca gaagatgtgg atggtgacaa atataaatcc cgtggtaacc gctataaccg
     1561 tgatgatatc actcaggcca taaatgctgg tcaatctcag cgatacatta cagaaaaata
     1621 tggtatttca cggagacagt atttctacat taagccattg attaataaag acaattaaaa
     1681 ataaccatct aagaaacggt agaatgcact ttacaggcag agttgcacct taatttgata
     1741 aattacctta ccagccagtg caactccctc tgtaacccgc atggttactg gcctcagaat
     1801 ggctatttta agaggtctga ggtgtgtttc ataggagatt taaaaatgaa taattaccca
     1861 atgcaaatta tggttgataa cgacactgct atgatggtgc aaagcttcat tgatgcaggt
     1921 atcgcaatta attttgataa attactaaag ctgatggctg ataatgtaga aaacatctct
     1981 gactttattc agtcggtgga gtttaacgag cctcgaatga tgctaccaat tacagacagc
     2041 aacatgaaaa gattggttat cgagcaaacg aacatataca gtatttcccc agagcaattt
     2101 ttgaaaggtg ctgtgacaat cctgtacagt gataatattc tggtgacgga ttctgtgagg
     2161 gttcactaat gggtttaata attgcttgga cgttgcttgg cttatttgtt ggttgtatta
     2221 ttaacaatgc ggtaaataag aattgatgtt atcaacctgt taaaaagctg ttgacaaata
     2281 accttaagca tggcataatt ttaattatgg gagagcaatt ctctcttatt tttctttcaa
     2341 taatccgatg gatggatatt ctgagttgga aaggtgcaat gcttttcgtc atggcctttc
     2401 aggttagtct gagaggctat ctcaaaaagt actcttgttc attcattatt gaatcctttg
     2461 cccttcacag aaatgtttag ggctttttca gtttttaaaa gcacacttgg caggttcttg
     2521 ttcagtcaga ctcttcacct ctgattgctg agtgtgcttc tataaattga aataacaaat
     2581 aaactaaggg gtgaacgaat gattagaaat gaacatgaaa tgcttatcga acacagacca
     2641 agtgcacttg atattcacga attaactttg cgtgaatctt ttgatagagc ttggcgggaa
     2701 tcacacgaag taaataaagg tccatatatt ccaccagaac cactcgagat tccaagagtt
     2761 gaaattgatt tttctatgaa cgaacggtgt gaacttgatt tgaagttgaa gctacaacgc
     2821 cgatatttcc aagctattaa agactctcaa gaagcaatga gaagtgttta cgagaaaatt
     2881 cagtccaagc ttgaagacga aattgacgaa atggttattt cgatggagct tcaaaccaat
     2941 tcttatgctt atttctctcg actagaccgt gacggttggg ggtatgaccc tgtagagatt
     3001 ggtaaagtga ttgcagattt accagaaggt caaagagttg ttcgtacaga aagagctgtc
     3061 agagcaccac atcgactgtc tctaattgtt gctgataaat cacaagctga gcttgaagaa
     3121 attgccaaag agaaactgat gaaagcaaga gaaaatgaga ttaatttgct gaaatcaaga
     3181 ctggctaagc tga
//
LOCUS       DAZOXR010000306         3183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZOXR010000306 DAZOXR010000000
VERSION     DAZOXR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.313"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUNN_004314"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUNN_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0578506.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUNN_004315"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUNN_004315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0578507.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHUNN_004316"
     CDS             complement(1715..1984)
                     /locus_tag="ACHUNN_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0578508.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUNN_004317"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUNN_004317"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0578509.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUNN_004318"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUNN_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0578510.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          771 c          769 g          811 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gca
//
LOCUS       DAZOXR010000307         3175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZOXR010000307 DAZOXR010000000
VERSION     DAZOXR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.314"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            533..2386
                     /locus_tag="ACHUNN_004319"
     CDS             533..2386
                     /locus_tag="ACHUNN_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0578511.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2537..2752
                     /locus_tag="ACHUNN_004320"
     CDS             2537..2752
                     /locus_tag="ACHUNN_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0578512.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2757..3071
                     /locus_tag="ACHUNN_004321"
     CDS             2757..3071
                     /locus_tag="ACHUNN_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR0578513.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          762 c          885 g          750 t
ORIGIN      
        1 cttccaggcc catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa
       61 tgctatgttg ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa
      121 aaaaatttcg catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct
      181 gattatcaaa acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca
      241 cccggcacca tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt
      301 tcttgtgggc aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa
      361 cgtgaagtaa tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg
      421 cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga
      481 cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt
      541 taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca
      601 aaaactgaag gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct
      661 gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg
      721 gtactacgtc atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc
      781 gcttccggat tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac
      841 agtgactccc ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct
      901 gcatgatgtt caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca
      961 gtacggctgt gtcggacagg gcttacatat tgccaaaaaa ctgcttccgt atatccctaa
     1021 taatgcgggg atcctgctgg taccatgctg tcgtggtggt tcggcattca cccagggcac
     1081 ggaggggaca ttcagcgagt ccacgggggc cagtcaggat tcggctcgct ggggagtggg
     1141 taagccgtta tatcaggatc tgcttttccg cacgaaggca gcattgcaga aaaacccgaa
     1201 aaacgttttg ctggcgatat gctggatgca gggggaattc gatatgacga atgccagtta
     1261 cgcccagcag ccagcagcat ttcttgcaat ggtacagcag ttccgtgctg accttgccgg
     1321 gctggcggcg cagtgtcacg gtggaagtcc ggcatcagtc ccctggattt gtggcgacac
     1381 gacatacgcg tggaaacaag aacacggtac gcaatatgaa gtggtatatg gtgcatataa
     1441 aggtaaagaa tcccagcaga tttattttgt tccctttatg accgatggta gcggagttaa
     1501 tacaccgaca aacaacccgt cagaagatcc tgatattgcc gggtctggtt attacggttc
     1561 ggcatcccga acgaacaaaa actgggtatc atcaaatcgc ccgacgcatt tcagctcatg
     1621 ggcgcgtcgt ggcattattc ccgatcgtat ggcaactgct attctgaacg tagccggtcg
     1681 caccttagcc ttcattagtg gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac
     1741 gccatcgggg ccgtctgaag atgcatccat acgcacaatc tccctgttgc cgacagccgg
     1801 agacgctgct gcgcagggct ggagcattaa gaatggcgga gttcagttgt cagatggtgt
     1861 atttaagatc accaagcaga gcaataaagc ctggtccctg acgcgcccgg tggatgacgc
     1921 agtctccctg ctgacacggg gtggcagact gagctgtaag tttcgactgt caggcgcact
     1981 gaccaacaac cagttcggtc tgggaattta tctgtatacc gatgtagcgt tacctgacgt
     2041 cgtggcgatg accgggactg gtaacccgtt cctgatgtcg ttcttcaccc agaccacaga
     2101 cggcaaactg aatctgatgc atcacaagaa agcaggaaac acaaagttgg gcgagttcgg
     2161 gaattacagt aacgactggc tgacgctgga gctggtgttc accgccggca gtgccacggt
     2221 tactccgaaa ctgaatggag tggctggccc ggcattccag gtcataaaag acagtctgac
     2281 actggggctg aatgcgctga cgctgacgga tattaccaaa aatgcagcgt atggcgttga
     2341 gatagaaagt ctggtgctgg agataaatgc accggcatca tcataaaaag tgagccagcc
     2401 aaatggaagg tatcgttaaa ctcaccggta gtgtcagtgg gtcgtctgag atgcctgcat
     2461 gagttatcag agccatcagt acttaactgg tggctttttt tattgttgtc agcttccgga
     2521 taacgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
     2581 gtcagcggtg ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca
     2641 gtgggcggca ataggcgtgc tggggagtct gctgtttggg ctgctgacat atctgactaa
     2701 cctgtatttc aaaatcagag aggaccgtcg taaggctgca cggggagagt aattcaatga
     2761 ctcaaaacta tgaactgatt gtgaaaggga tccgcaattt tgagaataaa gttacggtaa
     2821 ctttagcgtt acgggacaaa aaacgctttg acggtgaaat ttttgacctg gacatctcgc
     2881 tggaccgtgt tgaaggtgcc gcgctggagt tttatgaggc agcagccaga aggagcatca
     2941 gacaggtctt cctggatgtt gctgccgggt tatgtgaagg ggacgagctg ttgccagaaa
     3001 cgcgcccctg ttcagaggcg cggtatacca taaaaattaa cagttctgat aactcgatta
     3061 cgggttgtta gctttttgca gttggctttc cagtatcttt cattggtagc atcctgataa
     3121 atatccatga gcgcaaaaat caaatacggc ctgtcagctg ctgttctggc gctga
//
LOCUS       DAZOXR010000308         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZOXR010000308 DAZOXR010000000
VERSION     DAZOXR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.315"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1258)
                     /locus_tag="ACHUNN_004322"
     CDS             complement(<1..1258)
                     /locus_tag="ACHUNN_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0578514.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="ACHUNN_004323"
     CDS             complement(1415..1624)
                     /locus_tag="ACHUNN_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578515.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="ACHUNN_004324"
     CDS             complement(1647..2357)
                     /locus_tag="ACHUNN_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0578516.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="ACHUNN_004325"
     CDS             complement(2873..>3112)
                     /locus_tag="ACHUNN_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578517.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          913 a          653 c          507 g         1039 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcta
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAZOXR010000309         3046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZOXR010000309 DAZOXR010000000
VERSION     DAZOXR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.316"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUNN_004326"
     CDS             <1..173
                     /locus_tag="ACHUNN_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578518.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="ACHUNN_004327"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="ACHUNN_004327"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR0578519.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="ACHUNN_004328"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="ACHUNN_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="ACHUNN_004329"
     CDS             2191..>3046
                     /locus_tag="ACHUNN_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR0578520.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          697 a          710 c          836 g          803 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcaccg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggagaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAZOXR010000310         3032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZOXR010000310 DAZOXR010000000
VERSION     DAZOXR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.317"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..600)
                     /locus_tag="ACHUNN_004330"
     CDS             complement(<1..600)
                     /locus_tag="ACHUNN_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578521.1"
                     /translation="MFGMSITQSNGNVWMSPEFTPQNLINRGTMSTSKGSVFKTSIPS
                     NKSCFFFIKSSNKANMMFVHEFRDGYNVLRLNQVGGNPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGKMVYHGEMMPLDAEIITITNPQFTINMGYPCAIMPAMVGVYNYRRTPYDRP
                     VYVTMTGATGNQIYNGEWYSSNVTWNVKKLYTNKVLVINT"
     gene            complement(606..887)
                     /locus_tag="ACHUNN_004331"
     CDS             complement(606..887)
                     /locus_tag="ACHUNN_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578522.1"
                     /translation="MAYGTGIYNNKGVNVTGFLTPIFFLDRFTTTSGSKTYPNPPPGK
                     SLHAVCSLMPLNNDNHVNIPTPTITINGNTVSWSNLYKGLGSYIYTYWG"
     gene            complement(942..1511)
                     /locus_tag="ACHUNN_004332"
     CDS             complement(942..1511)
                     /locus_tag="ACHUNN_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578523.1"
                     /translation="MCEKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKQRADKLQETLDMVIETQGTHDSNKSETI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMVVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            complement(1651..1809)
                     /locus_tag="ACHUNN_004333"
     CDS             complement(1651..1809)
                     /locus_tag="ACHUNN_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0578524.1"
                     /translation="MLERFRLVTVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(1809..2234)
                     /locus_tag="ACHUNN_004334"
     CDS             complement(1809..2234)
                     /locus_tag="ACHUNN_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HFR0578525.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGGYMISYANGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRAQYEASPRRANAQALRELADVAGTDDA
                     VDVLCLCLDIADQDGIDEPEEQALKKIAQALQLSLDAYL"
     gene            complement(2328..2516)
                     /locus_tag="ACHUNN_004335"
     CDS             complement(2328..2516)
                     /locus_tag="ACHUNN_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578526.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            complement(2526..>3032)
                     /locus_tag="ACHUNN_004336"
     CDS             complement(2526..>3032)
                     /locus_tag="ACHUNN_004336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578527.1"
                     /translation="DGVHMARQTYFTSVTKRPRSLRQILAELFSGRVMSRLDELETTV
                     RLLNERLDNQASVVANVWAIVASGSSREAKNARPLVKEKNNKDSSNGKFSKKEAETNG
                     LRSHYSFTGDGSRSSRSEPFDAGFIHHHTSVDDNYHHSSRATCHSGWDDGGCDTSSSS
                     SYSGSCCD"
BASE COUNT          857 a          674 c          611 g          890 t
ORIGIN      
        1 tgtatttata accaggactt tatttgtata tagttttttt acattccatg taacattact
       61 tgaataccat tcaccattat atatttggtt tcccgttgcg cctgtcattg ttacgtatac
      121 aggtctgtcg taaggtgttc gtctatagtt atatactcca accattgcag gcattatcgc
      181 acacgggtat cccatattta tggtaaattg aggatttgta atagtgataa tctctgcatc
      241 aaggggcatc atctcaccgt gatataccat tttacctgcg ctattataca tggcaatacc
      301 atatcctgaa tgtggtaaca ccatatcaga aaaagcataa actgttatcg ttccggggtt
      361 tcctccaacc tgattcagtc tgagaacatt atacccatct ctaaattcat gaacgaacat
      421 catattcgcc ttattactgc tttttataaa gaaaaaacat gatttgttcg acggtattga
      481 agtcttaaaa acagaacctt tagacgtgga cattgtcccc ctattaatca aattttgcgg
      541 tgtaaattct ggactcatcc acacattacc atttgactga gtaattgaca tcccgaacat
      601 tattgttatc cccaatatgt atatatataa gatccaagtc ccttgtataa atttgaccaa
      661 cttactgtat tgccgttaat agtgatagtt ggtgtaggta tatttacatg gttatcgtta
      721 tttaaaggca tcaatgagca cacagcatgt aatgatttgc caggtggtgg atttgggtaa
      781 gtcttagagc ctgacgtggt tgtaaatcga tcaagaaaaa aaataggagt aaggaagccg
      841 gtaacgttaa ctcccttatt attgtagatc cctgtaccgt atgccatttt tcatccttgt
      901 ttttatcaac ataatatggc cgatcatgcg gccatatcag atcaattaga gctttgaaag
      961 gctttgtgtg gctgtgttgc gaggttatgt cgatggctta actcacgcat catgtcttca
     1021 aggcgactct ttgtgtcgtc gagttggtca gccattgctc ctaaaagctg acgaactacc
     1081 atcggatcgt cgctgtttag tgatggcatt ttataacccg cctgagaaga catcagattg
     1141 aatgctgaca tcaacatcgt tagagaggat ttaagcccgg caatttcacg atctttgctg
     1201 gcaataatcg tctcactttt gttactgtca tgagttcctt gagtctcgat caccatatcc
     1261 aaagtctctt gcaacttatc agctctctgt ttttcagcaa gataatgaac accgaaatga
     1321 tgtgctaaag ctacaacctg agttggctct tcaaaggtgg actcaaaatt aagtgctgta
     1381 accactctct caaaaatgga aacgtcttct attcctcgaa gaatggctaa cattttgaca
     1441 agttcattag catccatttc ttcgaatagg gcgttttgtt catcagctat tgctttgcat
     1501 ttctcgcaca tatgttttcc ttaatgctta aacaagttgt tttcttattg gctttattgt
     1561 gatgtggcat aaaaggggag caagctaaac gtaaaggtgc aaactctcta tttcggtcaa
     1621 cgaaacacaa tggccatttg acccttgtca ttaacacgtt gctttacgga ctagcggata
     1681 agcgaagtag atcgccagac caatgaggac gccatcagaa atgacagaca tcatctttcc
     1741 cgtaaaatcc accaacaccg ccatcaccag aagagcaatg acggtcacaa gccggaatct
     1801 ctcaagcatt agagataagc atccagtgac aactgaagcg cctgggcgat ctttttcagc
     1861 gcctgctctt ctggttcatc aatgccgtct tggtcggcaa tatcaaggca cagacaaagt
     1921 acatctactg catcatcagt cccggccaca tccgccagct cacgtaaagc ctgggcatta
     1981 gcacggcgcg gtgaggcttc gtattgagcg cggatattgg cgctcatctg ggctatctca
     2041 ccagcaaaag gagaaaatgc aggcagagct gcaattgttt tctcaagaat ggaaatttct
     2101 tttgcatcgc atgtaccgtt ggcataggag atcatgtacc caccccacac ggtggcttca
     2161 accgcatcac ggttctccat ttttttgact tcgataactg ctttacgagt tttctttttg
     2221 aagaaaccta acatgtgttt tccttttgtt attggtcaaa acaagttgtt ttctagttaa
     2281 ataaattgct tacgcaaaga cggtcaatgc atatgcacta acaaccgtca gaaataacca
     2341 agtaccgaac cgactggaaa aataaaaatc ccaactacac gagccaaggt catgccagcc
     2401 tgaaactgga gatcgccaga gcaaacaagt tttataatgt tcgataccca accggcggcc
     2461 atgagggcaa gaattgccag ccaaattttt ccaaagtgat ttgaaaacca gttcatacaa
     2521 ccgccttagt cacagcatga tcctgagtaa ctggatgaac ttgaggtatc gcatccaccg
     2581 tcatcccatc cagagtgaca ggtcgctcta ctggagtggt ggtaattgtc gtcgacggag
     2641 gtgtggtgat ggatgaatcc ggcatcaaaa ggctctgacc ggctggaacg gctaccgtcg
     2701 ccagtgaaac tataatgaga acgtaggcca ttggtttcag cctctttctt tgaaaatttt
     2761 ccattcgaac tgtccttgtt gtttttctcc ttcactaaag gccgtgcatt tttcgcttcg
     2821 cgtgaagaac cagaggcaac aatcgcccac acgttcgcaa caactgacgc ttgattatct
     2881 aaacgttcat tcagcaaccg aacggtagtc tctagttcat caaggcgtga cataacacga
     2941 ccgctaaata attcggccaa aatttgacgt aaagaacgag gacgtttagt tacagaagtg
     3001 aaataggttt gacgtgccat gtggactcca tc
//
LOCUS       DAZOXR010000311         3004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZOXR010000311 DAZOXR010000000
VERSION     DAZOXR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.318"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1230
                     /locus_tag="ACHUNN_004337"
     CDS             <1..1230
                     /locus_tag="ACHUNN_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR0578528.1"
                     /translation="DLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLR
                     PPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEGRVAGFESAYVALSRMKQHVQVYTDN
                     RQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLA
                     GGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKG
                     SGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGR
                     VWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPD
                     GKTELAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQM
                     ERDMVRDLQKEKTPGGD"
     gene            1250..1997
                     /gene="traX"
                     /locus_tag="ACHUNN_004338"
                     /pseudo
     CDS             1250..1997
                     /gene="traX"
                     /locus_tag="ACHUNN_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            2052..2612
                     /locus_tag="ACHUNN_004339"
     CDS             2052..2612
                     /locus_tag="ACHUNN_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0578529.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            2742..2954
                     /locus_tag="ACHUNN_004340"
     CDS             2742..2954
                     /locus_tag="ACHUNN_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0578530.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          698 a          752 c          997 g          557 t
ORIGIN      
        1 gatctgtggc cggtaaagga gccggacatg ctgacggtga tcatgcagcg cctgacggca
       61 ctggagcagt ccggtgagat gggccggaaa atggatgcct ttaaggagcg ggtgatccgc
      121 aacagtctgc ggccccctgc cgtgcccggt atcggacgca cggagaaata cagcagccgg
      181 ttgtttgacc cgtccgtcag actggctgcg gatatccggg ataacgaagg gcgggtggcc
      241 ggctttgagt cagcctacgt ggccctgtcg cgtatgaagc agcatgtgca ggtgtacacc
      301 gataaccgtc agggctggac ggatgccatc aacaatgccg tacagaaagg aactgcccac
      361 gatgtgtttg agccgaaacc ggaccgggag gtcatgaatg cagagcggct gttcagtacg
      421 gcgcgggagc tgcgggacgt ggcggcaggc cgtgccgttc tccgtcaggc ggggctggcc
      481 gggggagaca gtcctgcacg gtttattgct ccgggacgta aatatccgca accgtatgtg
      541 gcactgccgg cgtttgaccg taacggcaag tccgccggta tctggctgaa cccgctgacc
      601 acggatgacg gaaacgggct gcggggattc agcggtgaag gtcgggtaaa aggcagcggg
      661 gatgcgcagt tcgtggccct gcagggcagc cgtaacggag aaagcctgct ggctgataat
      721 atgcaggatg gcgtccggat tgcccgtgat aatcctgaca gtggtgtggt ggtaagcatt
      781 gccggtgaag gccgtccgtg gaatcccggt gccatcaccg atggccgcgt gtggggggat
      841 atcccggaca acagcgtcca gccgggagcc ggaaatggcg aaccggtcac ggcagaggta
      901 ctggcacagc ggcaggctga agaggccatc cgccgtgaaa cggaacgtcg cgcagatgaa
      961 attgtccgta aaatggcaga gaacaaacct gacctgccgg acggcaaaac agagctggct
     1021 gtcagggaaa ttgccgggca ggagcgtgac cgggctgcca taactgaacg ggaagccgcg
     1081 ctgccggaga gtgtgctgcg tgaatcacaa cgggagcggg aagcggtccg ggaggttgcc
     1141 cgggaaaatc tgctgcagga gcgtctgcag cagatggagc gggatatggt tcgtgacctg
     1201 cagaaagaga aaaccccggg cggagactga tacaggaaga caaacactga tgacaacgga
     1261 taacacgaac acgacacgta acgattcact ggctgcacgg accgatacct ggttgcagtc
     1321 atttctggtc tggtcacccg gacagcggga tatcatcaaa acggtggcac tggtgctgat
     1381 ggtactggac cacatcaatc tgatattcca gctgaagcag gaatggatgt ttctggccgg
     1441 gcgaggagcc tttccgcttt ttgccctggt gtgggggctg aatctgtccc gtcatgcgca
     1501 tatccggcaa ccggccatta accggctgtg gggatgggga attattgccc agttcgcata
     1561 ttatctggca ggctttccct ggtatgaggg gaatatcctg tttgcctttg cagtggcggc
     1621 acaggtgctg acgtggtgtg aaacgcgcag tgggtggcgt acagcagcgg ccattcttct
     1681 gatggccctg ttgggggcct ttgtccggca ccagttacgg cattgccggg ctgctgatgc
     1741 tggcagtcag ccaccgactg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg
     1801 cctgcctgct ggctgttatt ccggcgctta accttgccac cagtgatgca gcggcggtgg
     1861 ccggtcttgt gatgacggtg ctgaccgttg gtctggtgtc gtgtgcagga aaatcattac
     1921 cccgtttctg gcccggggat tttttcccgg tattctacgc ctgtcatctg gctgtgctgg
     1981 gcgttctggc gctgtgacgg gtgtggtatc tttgaccgta acaggatgat tcgtcagagg
     2041 cagaacacag catgacagag cagaagcgac cggtactgac actgaagcgg aaaacggaag
     2101 gggagacgcc tgtccgcagc cggaaaacca tcatcaatgt caccacgcca ccaaagtgga
     2161 aggtgaaaaa gcagaagctg gccgagaaag cagcccggga agcagagctg gcggcaaaaa
     2221 aagcgcaggc cagacaggca ctgtccattt atctgaacct gcccacgctg gatgaggccg
     2281 tgaataccct gaaaccctgg tggccgggat tatttgacgg tgacaccccc cggcttctgg
     2341 cctgcggtat ccgggacgtg ttactggaag acgtggcgca gcggaatatc ccgctctcgc
     2401 ataaaaaact gcgcagggcg ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca
     2461 tgaaagccgg tgcctgccgg tatgacacgg aagggtatgt gacggagcat atttctcagg
     2521 aggaagaagc gtatgcggca gcgcgtctgg ataaaatccg ccgccagaac cggataaagg
     2581 cagaacttca ggccgtgctt gatgagaaat aaaaaaaagc ctccatcaga gaggaggcag
     2641 ggaaataagg cttaaaagga aataacttcc gcttccgaaa ggaagccagt ccactgaatt
     2701 atttatcaca ataatactga caactaaagg gggacaagaa aatgaatgga ttcagaaaca
     2761 gttcacggaa cggccaggtc tggcgttacc agcgtgccgg cgggcgggcc gttattctgg
     2821 aagtcagtgg acgctggatg gaagcggcag aagcatggcg acgggctgcc tgtgttgcgc
     2881 ccaggacaga ctggcagcag tttgcccgaa aaagggctga acactgccac cggcgctgcc
     2941 ggggaagggt gtaacgaaaa aagcagcctt cattactcca ggagtcagaa ggctgaaagg
     3001 agtt
//
LOCUS       DAZOXR010000312         2994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZOXR010000312 DAZOXR010000000
VERSION     DAZOXR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.319"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUNN_004341"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUNN_004341"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0578531.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUNN_004342"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUNN_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR0578532.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2994
                     /locus_tag="ACHUNN_004343"
     CDS             2805..>2994
                     /locus_tag="ACHUNN_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR0578533.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          745 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc ttag
//
LOCUS       DAZOXR010000313         2976 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZOXR010000313 DAZOXR010000000
VERSION     DAZOXR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.320"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(150..1637)
                     /gene="yeeO"
                     /locus_tag="ACHUNN_004344"
     CDS             complement(150..1637)
                     /gene="yeeO"
                     /locus_tag="ACHUNN_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR0578534.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1798..1873
                     /locus_tag="ACHUNN_004345"
     tRNA            1798..1873
                     /locus_tag="ACHUNN_004345"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1831..1833,aa:Asn,seq:gtt)
     gene            complement(1911..2861)
                     /gene="cbl"
                     /locus_tag="ACHUNN_004346"
     CDS             complement(1911..2861)
                     /gene="cbl"
                     /locus_tag="ACHUNN_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0578535.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          872 a          795 c          628 g          681 t
ORIGIN      
        1 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
       61 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
      121 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
      181 ttttgcggtt cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
      241 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
      301 cagccaagca tgatgcccag cacataaccg acgacaaccc ggcaacccca catgctcaac
      361 atagaaaccc acatggcgta acgggcatcg cgagcacctt taaatccagc gggtagcacc
      421 catgaggcgg accaaatagg cataaataaa gcatttagcc aaatcagaat cacaacgaca
      481 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
      541 agccaggcga tggccgttaa tccaagagtg gaaagccaga acacatgccg caactgaatc
      601 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagag
      661 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg aaaacgcgat aaaatttccg
      721 gcaataacac tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
      781 aataacactg attcgacact cgcgggaata ccaatcccca tgacttccca gataatgcta
      841 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
      901 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccagccct
      961 gccccgacaa atcccagtcc cggccaggag aaaaggccgt aaatcaatat gccgctaata
     1021 ataatattaa gaatattcag gctaccgtta atcaatagcg gtattttcgt attccctgca
     1081 ccacgaagtg ccccgctacc aataagagtg atggcagctg ctggataact gagtaccgtc
     1141 agctccagat aagtcaacgc cagtgcttta acttctgtcg tggcatcacc cgcgacgaaa
     1201 tcaataattt gttcgccaaa atgatgaata agcgttgcca acagtacggc aaacaacgtc
     1261 atgatcacca atgactgccg cgtcgccacc ctcgctcgtc gtcgatcccg cttaccgaga
     1321 ctaaatgcca caacgacagt agtaccaaga tcgatagcag caaaaaaagc cataatgacc
     1381 atattgaagc tgtccgccaa tcccacgccg gccatcgcat cttttcccag ccagctgacc
     1441 agaaaagtgc tcagaacccc catcaacagg acacaggcat tctccatgaa gataggaaca
     1501 gcaagcgggg ttatctcgcg ccagaacaac actttgtagc tcttgcgttt agcgtgccag
     1561 cgagtgccgt gaacaacctg gcgtaaagca gaggagatat tcaaagccga ccttaattgc
     1621 agaaagtgaa accacatttc aaataatgag ggagaatcag caaagctgca aagattttcg
     1681 ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa acggcaaaca gttggggaat
     1741 ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga tgtgcctcaa cagcgattcc
     1801 tctgtagttc agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca
     1861 gtcagaggag ccaaatttct gaaaagcccg cttttatagc gggctttttg ctatatctga
     1921 taatcaattt cctcttcact gttttccatc acctgccgct tgatatcctc aactgacagt
     1981 cctgcattac aaagttccag aaagcgccag acatagttac gctgaagttg tcctcgcttc
     2041 agtcccaacc agacagtatt agcatcgaaa agatgccgcg tatccagacg gattaaattc
     2101 tcttcctctt gttcgccgct ggattgttcg gcaactaatc cgatcccaag cccaagagca
     2161 acataggttt taatgacatc agaatcctgc gcacttaata caatatctgc cagcaaacct
     2221 ttgcgggcaa atgcgtcatc aatacgtgag cgccccgtaa tcccctgtcg gtaagtgatt
     2281 aacggccact tcgctattga ttccagcgtc aatggtgtaa tttgcgttaa gggatgatcg
     2341 agtggaacaa gcaaactatg gtgccaacga aaccacggga aggcgacgag ctgcgggtca
     2401 ttactcaaac gctcgctggc gataccaata tcagcttcgc cattttgcaa caacgtcgca
     2461 atttcctgtg gcgtcccctg gattagctcg agccgaacct ccgggaaaag ttcgcgaaaa
     2521 gctttaatga cctctggcaa gctataacgt gcctgagtat gcgtcgttgc aatagtgaga
     2581 acgccagacg tatcgttggt aaacaggtct gcaagccgac gaacattact ggcttcattc
     2641 agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt cagtcatgcc cagcagtcgc
     2701 ttacctcgtc gaacaaatat ttcgatgcca agttcatcct ccagttcccg aatatgacgg
     2761 ctgacgccgg actgtgaggt aaaaagcata ttcgcaacct ctgtcaggtt gtaatcctga
     2821 cgtgcagcct cgcggattat ctttagttgt tggaaattca cggtaaactc cgggcagttc
     2881 agatttcccg ttattgttaa agtctaatgc ccggcataac aaataataaa aacccgcatc
     2941 ttattccatc ccgatataac gcttagctca ccaatt
//
LOCUS       DAZOXR010000314         2973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZOXR010000314 DAZOXR010000000
VERSION     DAZOXR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.321"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            91..804
                     /locus_tag="ACHUNN_004347"
     CDS             91..804
                     /locus_tag="ACHUNN_004347"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0578536.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            836..1594
                     /locus_tag="ACHUNN_004348"
     CDS             836..1594
                     /locus_tag="ACHUNN_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578537.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1640..>2973
                     /locus_tag="ACHUNN_004349"
     CDS             1640..>2973
                     /locus_tag="ACHUNN_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0578538.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          664 a          730 c          804 g          775 t
ORIGIN      
        1 cacaattaac cattgtcaca ataaggttga acggatattc tgagcacgga ccacgtaata
       61 ttcaatacat tatttactgc cgggtttttc gtgaatcaac gtaatatgtc aataattaat
      121 tccacgccag tgcgtgttat tgccattgta ggatgtgatg gttcaggtaa atcgcccctc
      181 acggcaagcc tggtaaatga actggcagca agaatgccaa cagaacacat ttatctcggg
      241 caatcgtccg ggctaattgg cgaatggatt tcacagctcc ctgttattgg cgcacctttt
      301 gggcgttatc tgcgaagtaa agcggcacat gtgcacgaaa aaccctcaac accgcctggc
      361 aatattactg ctctggttat ctatctgctt tcctgctggc gggcgtacaa gtttcgcaaa
      421 atgttgtgta aaagccagca aggctttctg ctcatcaccg accgctaccc gcaggttgaa
      481 gtgccgggtt ttcgctttga tggcccgcaa ttggcaaaaa ccacgggcgg taacggttgg
      541 ataaaaatgt taaggcagcg cgagctgaag ctgtaccaat ggatggcatc ttatttgccc
      601 gtattgttga ttcgtcttgg cattgatgaa caaaccgcgt ttgcgcgtaa acctgaccac
      661 caactggcag cgttacagga aaaaatcgcc gttacgccgc aactgacttt caacggcgcc
      721 aggatccttg aactggatgg tcggcaacca gccgatgaaa tcctgcaagc gtcactacgt
      781 gcaattcacg ccgcgctttc ctgatcacca aaatggatag gggatgattt aacgaatgga
      841 tgcactacaa acacaaactg ttaatagtac aaccgcgccg cagcccagct acattccggg
      901 gctaattgcg gtggtcgggt gcgatggcac cggtaaatcc acgctgacca ccgatctggt
      961 gaaatcattg caacagcact ggcaaaccga gcggcgctat ctggggctgc tctcgggcga
     1021 agacggcgac aaaatcaaac gattaccgtt ggttggcgtc tggctggaac ggcgactggc
     1081 ggccaaatcc tcgaaaaccc aaagcatgaa aaccaaatct ccggcgctat gggcggcggt
     1141 gattatgtac tgcttatcgc tgcgaagaat ggcgaatcta cgcaaggttc agcgactggc
     1201 gcaaagtggc gttctggtgg tcagcgatcg cttcccgcag gctgaaattt cgggctttta
     1261 ttacgacgga ccggggattg gcgtcgaacg tgcgaccggg aaaatcagca tgtttctggc
     1321 gcagcgcgaa cggcgtttat accaacaaat ggcgcaatat cgcccggaat taattattcg
     1381 cctgggcatt gatattgaga ctgccatctc ccgcaagcct gaccatgact atgccgagct
     1441 gcaggacaaa atcggtgtca tgtcgaagat tggctataac ggcacaaaaa ttcttgagat
     1501 tgattccaga gcgccctaca gcgaagtgct ggaacaggca cagaaggcgg tttctctggt
     1561 tgccatcgtt tctgaccgcc gaagtttaac ttaaggctgg cgggatactt tttactatca
     1621 acgataaggt caggctgaat tggcgggttt taacatcaaa cattggtttg cagatggcgc
     1681 gtttcgcacc attattcgca atagcgcctg gttaggctcc agtaatgtcg tgagcgcctt
     1741 gctggggctg ttggcgctct cgtgtgccgg taaagggatg acgcccgcca tgtttggcgt
     1801 actggtgatt gtgcaatcgt acgccaagtc gatcagcgat tttattaagt ttcagacatg
     1861 gcagctggtg gttcagtacg gaacgccagc cttgaccaac aataatccgc agcaatttcg
     1921 caatgtcgtc tcattttcct tctcgctgga tatcgtcagc ggcgcggtgg cgattgtcgg
     1981 cggcattgcc ttactgccat tcctttccca ttcattaggt ctggatgacc aaagcttttg
     2041 gctggcggcg ctgtattgca cgctcattcc ttcaatggct tcctccacgc cgaccggcat
     2101 tctgcgtgcg gtagatcgct tcgatttaat tgctgtacag caggcgacga aaccttttct
     2161 gcgcgcagcg gggagcgtcg tagcctggta ttttgacttt ggttttgcgg gttttgttat
     2221 tgcctggtac gtgtcgaatc tggttggcgg caccatgtac tggtggtttg cagcgcgcga
     2281 attacgccgc agaaatatcc ataatgcctt caaattgaat ctgtttgagt ctgcccgaca
     2341 catcaaaggc gcgtggagtt ttgtctggtc aaccaacatt gcccactcca tctggtcggc
     2401 gcgtaactcg tgcagcacag tgctggtggg gatcgtgtta ggacccgctg ccgccgggtt
     2461 atttaaaatc gccatgacat tctttgacgc cgccggaacg ccagcgggtt tgctgggtaa
     2521 aagtttttac ccagaggtga tgcgtttaga tccgcgcacc accagaccgt ggttgttagg
     2581 tgtgaagtcc ggtttactgg cgggcggaat cggtattctg gtggcgctcg cggtgttgat
     2641 cgccggcaag ccgctcattt cgctggtgtt tggcgttaag tatctcgaag cgtatgacct
     2701 gattcaggtg atgctgggcg caattgtgat ttcgatgctg ggtttcccgc aggagtcatt
     2761 gctattgatg gcgggaaaac agcgcgcttt tctcgtggcg caaaccatcg cctcaatcgg
     2821 ctacatcgta ttgctgttca tgttctgtca tctgtttggt gtgctgggag cagcgtttgc
     2881 ttacttcggt gggcaatgtc tggatgtggt gctctcgtta attccaactt taaaggcatt
     2941 ttttcagcgt cattccttgc tttataacgc agc
//
LOCUS       DAZOXR010000315         2966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZOXR010000315 DAZOXR010000000
VERSION     DAZOXR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.322"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHUNN_004350"
     CDS             <1..216
                     /locus_tag="ACHUNN_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0578539.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(161..298)
                     /locus_tag="ACHUNN_004351"
     CDS             complement(161..298)
                     /locus_tag="ACHUNN_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR0578540.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(271..369)
                     /locus_tag="ACHUNN_004352"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="ACHUNN_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(358..597)
                     /locus_tag="ACHUNN_004353"
                     /pseudo
     CDS             complement(358..597)
                     /locus_tag="ACHUNN_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            696..1586
                     /locus_tag="ACHUNN_004354"
                     /pseudo
     CDS             696..1586
                     /locus_tag="ACHUNN_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1589..2122
                     /locus_tag="ACHUNN_004355"
     CDS             1589..2122
                     /locus_tag="ACHUNN_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0578541.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="ACHUNN_004356"
     CDS             complement(2151..2678)
                     /locus_tag="ACHUNN_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0578542.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2966)
                     /locus_tag="ACHUNN_004357"
     CDS             complement(2680..>2966)
                     /locus_tag="ACHUNN_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0578543.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          838 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaa
//
LOCUS       DAZOXR010000316         2944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZOXR010000316 DAZOXR010000000
VERSION     DAZOXR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.323"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            45..1120
                     /locus_tag="ACHUNN_004358"
                     /pseudo
     CDS             45..1120
                     /locus_tag="ACHUNN_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1136..1884
                     /locus_tag="ACHUNN_004359"
                     /pseudo
     CDS             1136..1884
                     /locus_tag="ACHUNN_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            1886..>2944
                     /locus_tag="ACHUNN_004360"
     CDS             1886..>2944
                     /locus_tag="ACHUNN_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578544.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAZOXR010000317         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZOXR010000317 DAZOXR010000000
VERSION     DAZOXR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.324"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHUNN_004361"
     CDS             37..264
                     /locus_tag="ACHUNN_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0578545.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHUNN_004362"
     CDS             complement(265..639)
                     /locus_tag="ACHUNN_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR0578546.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUNN_004363"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUNN_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHUNN_004364"
     CDS             complement(2099..2674)
                     /locus_tag="ACHUNN_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0578547.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          696 c          688 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt agatttgttg tctatagcta
     2761 tagtgactct tctatgtaga cattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZOXR010000318         2927 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZOXR010000318 DAZOXR010000000
VERSION     DAZOXR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.325"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1511)
                     /locus_tag="ACHUNN_004365"
     CDS             complement(<1..1511)
                     /locus_tag="ACHUNN_004365"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR0578548.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINF"
     gene            complement(1532..2158)
                     /locus_tag="ACHUNN_004366"
     CDS             complement(1532..2158)
                     /locus_tag="ACHUNN_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0578549.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(2614..2823)
                     /gene="fumD"
                     /locus_tag="ACHUNN_004367"
     CDS             complement(2614..2823)
                     /gene="fumD"
                     /locus_tag="ACHUNN_004367"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0578550.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          797 a          735 c          582 g          813 t
ORIGIN      
        1 ataaagttga tatgggtatg cgtcatgcag tcacggttga acatacagtt aacaatggaa
       61 ccgacctgcg ctgacgcttc attggcatgg tgaaccggat agccaaacgg cgaccagagt
      121 ttatttttcg cgtagcccca gtactcttca cccaaattcc agcgttctgc gatggcatat
      181 gaaccatcag ccaggtgact cagctcaccc acacgatgcg ccagacggta gtaaaaatct
      241 ttaattttaa tgaagttaac gtcacccgct tccagttgat cccagcgaat ttccgcatac
      301 tcttcagcag gcagaacacg cttgaacaca cctttgctgt aacaataggt aaagtcgcga
      361 tgcaactgcc cgtagttaca ccataggccg tagtcatcga acagattcag acccaccagg
      421 ttaccaatca cacgaccatc gtctttatct tcaaaatctt tcggaccgtt cgggaagatg
      481 gtggtatgga caaagttggc tacacaagtg ttaccacctg tgctgggcac gccaaactct
      541 ttcacccgag gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccactc
      601 atttttactg tgtatttctc tgccgccgga cctaagtcaa aaacggattt ataggtacga
      661 aagccgaccg tgttctgatt gcctggcgga atttcccccg tttcaatcgg accaccttca
      721 gccgcgcccc aaaacagccc tttacgtgct gtccaacgtg acttgggatc tgaatactct
      781 gcccacgatt gcggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc
      841 atcatgtaat cgttgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc
      901 ccttcaaccg caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg
      961 ccgctgtggt tacggctatt caacatgcca gagagaggaa caaggttttc cccagcctga
     1021 ccaatagccg ccacacaggt ttccggactg gtcaatcgac aaatttcttc cgtcgttgcg
     1081 cgcgtccctt ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc
     1141 ttcagccata ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat
     1201 ttcatttgcg ctgcaaaaaa gccacccata tgggcatcga cgactaaatt tcctttggta
     1261 aaagtagaaa gtgaggtgat atttacgcga gaactacagg gggcaccaga tccagttaat
     1321 gggccggtag caaagactaa tttattcgct tcatcgaaag gtttcgtgcc tggcggtact
     1381 tcgtcataca taattttgta gccgaagccc atgccaccga caaaactttt aaacttactg
     1441 gaatcttcga gggtaatatt tcctgtcgtg agattgactc ttaatatatt acctgtccaa
     1501 ccgttagcca tgattttttc ctttgcaaga ttcacacagt aatatctttc cactcgataa
     1561 tttttaacgc ccctgttggg caggcgtttg cgcattcacc gcataacaca catttcgagg
     1621 attttttact ttcggtattt acggtggcca tcatccacgg gcatgccgta gtacaggcgc
     1681 tacagccaat acagcgttta tgatcgacgg taatacagcc ttctttctgc tgccaggtaa
     1741 tcgcaccaat cgggcagacg ttcatgcatt gcggctcttt gcattgacgg caggtgtccg
     1801 cggtatagtt gagatcgcca tacaggccgc cgccagagcc aaccccgttg tcgccaaaga
     1861 aataattgcg atggattttg atacgggaga agaatgtccc tactgagcca tcgttgaagt
     1921 tggtacaaga gatttcacag cggtggcaac ccgtgcatcg cgctcgctgg gtcaccagca
     1981 cccctttggg tgtatttatc aaccctactg tgccgctatc aatatcttcc tgtttgcaac
     2041 ccaaaagtga cagtaacgca ggggcaattg ttagaccagc aaggcctttt cctgatatac
     2101 gcaagaattc taatcgcgtt aagccaatat ctaatagtgg acgatcaacc gggttcattt
     2161 atttcgcatc ctcttcgcaa acacagtgct gacaattcag cagcctgata ttaagcattc
     2221 aactaatacg ctgctgactc tgtccccaac accagatatt ttccctaacc cgatagtgta
     2281 tgagaaaaga tttaacgccg ataaaccaat cctttatggt taggagaaaa aataatgtga
     2341 ttattctgag cccttaacat tgatcgttat caattaaaat tacaaagcaa gaaattataa
     2401 gtgaactgat atttattatc aattgaaata aatttaactt aagcgacaat tatttgtgac
     2461 gttcattgca gaaaataaaa ataatattat cgttatagat taaaatacat accgcaattc
     2521 gtataatttc agcagttgtt atttagaatg attaactgga ccgcaactga agcaagaata
     2581 tcaggatgaa gcttaccgcc tcatcctgaa tggtcatttt accagtgcgt taatgacggt
     2641 ttccatcgcc tgcttttcca gctcactgcg ctgaatgcgt ttattggcta atgcagtacg
     2701 taatacgcct gcgataacaa tatgctttgg ttcctgcccc agatcgcgca tttccagcac
     2761 aactttaccg acaactctgc acatttctcg gtacagttca tcctcttttg tccgatttcc
     2821 cattctttat acctatttat catgccaact atcagcatat atcaatctaa ccaattaaac
     2881 aaaaatcaaa caaaatcaga caaataacgc gataaattat tttaact
//
LOCUS       DAZOXR010000319         2917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZOXR010000319 DAZOXR010000000
VERSION     DAZOXR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.326"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUNN_004368"
     CDS             <1..493
                     /locus_tag="ACHUNN_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0578551.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUNN_004369"
     CDS             493..1083
                     /locus_tag="ACHUNN_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR0578552.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHUNN_004370"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHUNN_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHUNN_004371"
     CDS             2170..2658
                     /locus_tag="ACHUNN_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0578553.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2917
                     /locus_tag="ACHUNN_004372"
     CDS             2682..>2917
                     /locus_tag="ACHUNN_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0578554.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          831 g          692 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacga
//
LOCUS       DAZOXR010000320         2887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZOXR010000320 DAZOXR010000000
VERSION     DAZOXR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.327"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHUNN_004373"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHUNN_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUNN_004374"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUNN_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR0578555.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="ACHUNN_004375"
     CDS             2354..2758
                     /locus_tag="ACHUNN_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR0578556.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..>2887
                     /locus_tag="ACHUNN_004376"
     CDS             2811..>2887
                     /locus_tag="ACHUNN_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578557.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNH"
BASE COUNT          858 a          607 c          620 g          802 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcacca
//
LOCUS       DAZOXR010000321         2880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZOXR010000321 DAZOXR010000000
VERSION     DAZOXR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.328"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUNN_004377"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUNN_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0578558.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUNN_004378"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUNN_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0578559.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="ACHUNN_004379"
     CDS             1649..2098
                     /locus_tag="ACHUNN_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0578560.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUNN_004380"
     CDS             complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUNN_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0578561.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUNN_004381"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUNN_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0578562.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2880
                     /gene="yncL"
                     /locus_tag="ACHUNN_004382"
     CDS             2786..>2880
                     /gene="yncL"
                     /locus_tag="ACHUNN_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0578563.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          693 g          758 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttatttg
//
LOCUS       DAZOXR010000322         2739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZOXR010000322 DAZOXR010000000
VERSION     DAZOXR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.329"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHUNN_004383"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHUNN_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR0578564.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="ACHUNN_004384"
     CDS             1322..2374
                     /locus_tag="ACHUNN_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR0578565.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAZOXR010000323         2727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZOXR010000323 DAZOXR010000000
VERSION     DAZOXR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.330"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHUNN_004385"
     CDS             complement(423..680)
                     /locus_tag="ACHUNN_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578566.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHUNN_004386"
     CDS             complement(677..1174)
                     /locus_tag="ACHUNN_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR0578567.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHUNN_004387"
     CDS             complement(1376..1834)
                     /locus_tag="ACHUNN_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0578568.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHUNN_004388"
     CDS             complement(1831..2328)
                     /locus_tag="ACHUNN_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0578569.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUNN_004389"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUNN_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR0578570.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacc
//
LOCUS       DAZOXR010000324         2725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZOXR010000324 DAZOXR010000000
VERSION     DAZOXR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.331"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            30..2546
                     /locus_tag="ACHUNN_004390"
     CDS             30..2546
                     /locus_tag="ACHUNN_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0578571.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2725
                     /locus_tag="ACHUNN_004391"
     CDS             2557..>2725
                     /locus_tag="ACHUNN_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0578572.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREF"
BASE COUNT          779 a          657 c          630 g          659 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga gttta
//
LOCUS       DAZOXR010000325         2704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZOXR010000325 DAZOXR010000000
VERSION     DAZOXR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.332"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..248
                     /locus_tag="ACHUNN_004392"
     CDS             <1..248
                     /locus_tag="ACHUNN_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR0578573.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(288..1496)
                     /locus_tag="ACHUNN_004393"
     CDS             complement(288..1496)
                     /locus_tag="ACHUNN_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0578574.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1504..2005
                     /gene="tnpA"
                     /locus_tag="ACHUNN_004394"
                     /pseudo
     CDS             1504..2005
                     /gene="tnpA"
                     /locus_tag="ACHUNN_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2023..2691)
                     /locus_tag="ACHUNN_004395"
                     /pseudo
     CDS             complement(2023..2691)
                     /locus_tag="ACHUNN_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          626 a          665 c          664 g          749 t
ORIGIN      
        1 tggctgccct gcccgtaagt tggatccaga tgctggcagg tctggcgctg ttgagtacca
       61 ttggcggcag tttgtatcag gcgctgcata atgagcgtga gcgtgagcgc gacgcggcgg
      121 tggtggcatt tctggtaacg gcaagtggat tgacgctggt cgggattggt tctgcgttct
      181 ggggattaat tgccggaggc gtttgttacg tggtgttgaa tttaatcgct gacagaaacc
      241 gatattgaca tcctccacgc cctgaaggac gtggatccct gctacgttca ggctgtcgcc
      301 tgaatcattt cggtgggttc ctgcttcaac gggcggcctg actgcaccat ctctccacag
      361 gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcac catttacatc ggcgttcgct
      421 gtatatccac atacctggca tctgaattta ctttgtgaca ggcggttctc tttcgctgta
      481 tgaccacagt acgcgcaacg ctggcttgta tacgctggcg gaacagcaag cacctgaccg
      541 ccacgccaga gctgcttgta ctcaagctgg cggtgcattt cataccagcc ctgatccagt
      601 atcgaacggt ttaaacctga ttttgcccgg acattgcgcc ccggctggct gaccgtaccc
      661 gctgctgact ttgacatgtt gctgaccttc aaatcctcaa tgactatcat tgcgtggttt
      721 ttgctgacgg ccgttgtgac tttgtgaagg tagtccctgc ggatatttgc gatacaggaa
      781 tgcagtcgct gtattttgcg tttctgcttc tgccagttgt tgctgaattt gaccttgcgg
      841 cttaactgtc gctgaagtct cgccagcgtc ttctggtttt tctgaaaact gtttacaggc
      901 tcaaagactg tgccatctga cagcgtggcg agtttagcca cgccagcatc cagcccgacc
      961 attgatgctg aagggtgagc cggagtggat acttcacttt ctgtctgaat actgatgtac
     1021 cacgtaccgc aggactggct gacagtgaca tttttcacaa cacccgtgac ctgacggcta
     1081 ttccggtagc gcatccagcc cagtttcggc agaaaaatac ggctgttttc ctgatcgagc
     1141 ttaacgccct gcgggtagcg gaatacatca ttctgtcccc gctttttgaa tcggggaaaa
     1201 gcagcccgct tccggaagaa gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc
     1261 aatggctgtg agggagaatc tttaagccat tgcgtttcag tggcattttt ccactcaacc
     1321 agccaggaag ccattttccc gtaagggatg tatttattac cggcctcatg attctcattc
     1381 tgacgtgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca
     1441 cactcctgtt gaccacctgg tcttaactgg aatttaaatg cctgtaatcg tttcacaggt
     1501 caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt
     1561 ttcctgatgc atgttcacct ggtctttgtc actagatacc gacgccagat tttttgacgc
     1621 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag ctgaactggt
     1681 tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactaacctc ccaaactggc
     1741 gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt aggttactgc gacgagatcg
     1801 accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg gctattttgc
     1861 cagtagctgc ggaggtgtgc caatatccgt catccgccaa tacattgaac aacagcaaac
     1921 acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggatggg gttttacggc
     1981 gcaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt tgttaacgtc
     2041 aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt cgaaagccat
     2101 tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt tgataaccgg
     2161 tttattagtc gctgcatcaa tcagctcacc ttcaaaatag agtcgagtat ccatggtacg
     2221 gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca cttcatagaa
     2281 ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa tcagactacg
     2341 cggcccagcg gtggtaacca gtggtttacg ctgcgctatc gcttctttca tttcggtgtt
     2401 ggtatagttc aaaattttat ccagaacttt ctgccctacc taggtagaag gttttggcac
     2461 cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat caaaactcgg
     2521 gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta aatcagagta
     2581 attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac aaccagacag
     2641 agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca tgaatagttt
     2701 cctg
//
LOCUS       DAZOXR010000326         2698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZOXR010000326 DAZOXR010000000
VERSION     DAZOXR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.333"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(28..411)
                     /gene="quuD"
                     /locus_tag="ACHUNN_004396"
     CDS             complement(28..411)
                     /gene="quuD"
                     /locus_tag="ACHUNN_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR0578575.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(497..637)
                     /locus_tag="ACHUNN_004397"
     CDS             complement(497..637)
                     /locus_tag="ACHUNN_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR0578576.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(634..996)
                     /gene="rusA"
                     /locus_tag="ACHUNN_004398"
     CDS             complement(634..996)
                     /gene="rusA"
                     /locus_tag="ACHUNN_004398"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR0578577.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(993..1283)
                     /locus_tag="ACHUNN_004399"
     CDS             complement(993..1283)
                     /locus_tag="ACHUNN_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0578578.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1276..1446)
                     /locus_tag="ACHUNN_004400"
     CDS             complement(1276..1446)
                     /locus_tag="ACHUNN_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR0578579.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1446..1901)
                     /locus_tag="ACHUNN_004401"
     CDS             complement(1446..1901)
                     /locus_tag="ACHUNN_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR0578580.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            2166..2543
                     /locus_tag="ACHUNN_004402"
     CDS             2166..2543
                     /locus_tag="ACHUNN_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578581.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT          734 a          644 c          604 g          716 t
ORIGIN      
        1 aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga tatccatctc
       61 taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct tctgtaacct
      121 tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga atgtcatacc
      181 gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt tcagacgggc
      241 catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag attttacttt
      301 tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat cttcatgatt
      361 attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca tcaacttact
      421 ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca gtattagttg
      481 attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa ttcttcagta
      541 ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc ccccagcggt
      601 ggcgaagaaa ttctgccata taaaactcaa acatcattca ttccccattt cggtgatggt
      661 cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat caacgacctg
      721 agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt tttgcagatt
      781 atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt tcactggcat
      841 agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat cgcggtatgc
      901 ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc ggttattgct
      961 cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc ctctttcagc
     1021 cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata ctcagcatcg
     1081 acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat ggtggcaatc
     1141 aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg tgccagtact
     1201 gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc ccgaccacgc
     1261 gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc attttttatc
     1321 aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca gctcaggctt
     1381 acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa taagcggtgt
     1441 agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg cggaattgtc
     1501 aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat gtacatctcg
     1561 ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc gccggacacg
     1621 acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc gtctttgacc
     1681 cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat ctccgtgtac
     1741 cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg cttaacctgc
     1801 aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat ggccgcgaag
     1861 ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc ctaacggaaa
     1921 ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg atgagttcag
     1981 attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg ttcaggctcc
     2041 gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa gccaaaacag
     2101 tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa ctatggatct
     2161 aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag aaagagcttt
     2221 caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct aaggatggtg
     2281 gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct attgctggta
     2341 attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt ggtggtgatc
     2401 tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca atatttttaa
     2461 ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac gtagagggca
     2521 tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca agtcagtggt
     2581 gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc tgattatttg
     2641 cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc ggtagcaa
//
LOCUS       DAZOXR010000327         2690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZOXR010000327 DAZOXR010000000
VERSION     DAZOXR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.334"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            44..769
                     /locus_tag="ACHUNN_004403"
     CDS             44..769
                     /locus_tag="ACHUNN_004403"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HFR0578582.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHUNN_004404"
     CDS             complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHUNN_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0578583.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="ACHUNN_004405"
     CDS             1604..1798
                     /locus_tag="ACHUNN_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0578584.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            2296..2457
                     /locus_tag="ACHUNN_004406"
     CDS             2296..2457
                     /locus_tag="ACHUNN_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0578585.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAZOXR010000328         2608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZOXR010000328 DAZOXR010000000
VERSION     DAZOXR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.335"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            111..443
                     /locus_tag="ACHUNN_004407"
     CDS             111..443
                     /locus_tag="ACHUNN_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0578586.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUNN_004408"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUNN_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0578587.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHUNN_004409"
     CDS             complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHUNN_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR0578588.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT          682 a          678 c          619 g          629 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcc
//
LOCUS       DAZOXR010000329         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZOXR010000329 DAZOXR010000000
VERSION     DAZOXR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.336"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUNN_004410"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUNN_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0578589.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUNN_004411"
     CDS             complement(390..1178)
                     /locus_tag="ACHUNN_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0578590.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUNN_004412"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUNN_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0578591.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUNN_004413"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUNN_004413"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0578592.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUNN_004414"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUNN_004414"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0578593.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZOXR010000330         2553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZOXR010000330 DAZOXR010000000
VERSION     DAZOXR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.337"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHUNN_004415"
     CDS             <1..343
                     /locus_tag="ACHUNN_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0578594.1"
                     /translation="FLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKT
                     KEIVNKLPGYVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDL
                     RIAIKKLGWNN"
     gene            complement(415..1419)
                     /gene="argF"
                     /locus_tag="ACHUNN_004416"
     CDS             complement(415..1419)
                     /gene="argF"
                     /locus_tag="ACHUNN_004416"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0578595.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1581..1997
                     /gene="rraB"
                     /locus_tag="ACHUNN_004417"
     CDS             1581..1997
                     /gene="rraB"
                     /locus_tag="ACHUNN_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0578596.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(2043..2546)
                     /locus_tag="ACHUNN_004418"
     CDS             complement(2043..2546)
                     /locus_tag="ACHUNN_004418"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0578597.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          688 a          585 c          610 g          670 t
ORIGIN      
        1 ttttttgaag gattttttca tgaagcaacc tgattttgct aagtggtact tctatcaatt
       61 acttaagtgt tatgagggcg aacagctgta tcttaatgag ttaggatatg tttatggtga
      121 tgaagaaaaa acaaaagaaa ttgtaaataa acttccgggt tatgtcgtta agatttttga
      181 agaaaagatg ggtaatgaat taaaaattag aacaagaatg atgaaaatcc tccggaatgg
      241 caaaatcaat atatatggat atattaatga agaacagtta ggaaaattaa atcctccgga
      301 agatttacgt atagcaataa aaaaattggg gtggaataat tgaactatca ctgcataaaa
      361 taatgtgacg ccgggatgtt tttgtatttc ccggcatctt tatagcgata tcaattattt
      421 actgagcgtc gcgaccatca ccgctttgat ggtatgcatg cggttttccg cctgatcaaa
      481 tacaatgctg gcggcagatt cgaagacctc atcagtcact tccattccgc catgtaggcc
      541 aaactcttcc gccatttttt tgccaagcgt cgtttggtcg tcatgaaacg cgggcaggca
      601 gtggaggaat ttgacctccg ggttaccggt caactgcatc atcttgctgt tcacttgata
      661 atcacgcagc aatgcaatgc gttcagccca tttctctttt gcttcaccca tcgacaccca
      721 tacatcggta tagataaagt cagcaccttc aactccttta gctacatctt ccgtcagcgt
      781 aatattcccg ccattttgtt gtgccagggc gcggcattcc gtaaccagcg cagcttccgg
      841 ccagcatgcc tgtggcgcga ccagacgcaa atccagaccg gtaagcgctg cagcttcgag
      901 catcgaattg cccatgttgt tacgtgcgtc acctgcatag actagcgtca tttcgttgaa
      961 cgttttgccg ggcaaatgct cctgcatggt gaggagatcc gccagcagct gcgtggggtg
     1021 aaactcattg gtcaggccgt tccataccgg cacgccagcg tattccgcca gtgtttcgac
     1081 aatctcctga ccatagccgc gatactgaat accgtcatac atgcgaccaa gcacgcgggc
     1141 agtgtcttta atcgactctt tatgaccaat ttggctgccg cttgggccga gataagtaac
     1201 acgagccccc tggtcatatg cggcaacttc gaaagagcat cgggtacggg tcgagtcttt
     1261 ttcgaagatg agcgcgatgt ttttaccagt gagtttggct tcttctttac cgcttttctt
     1321 atcggctttc agcttcgcgg ctaactgcag caggctgttg agttcagctg gcgtgaaatc
     1381 gagtaatttc aggaaatgct tatgataaaa cccggacata ggtccctcct gtggctaacg
     1441 ccttaatgaa ttaaaattca atttatatgg atgattattc atttgcaagt ctaaagcata
     1501 aatctttatc acaaaggtgg aggcaatgtc agtggtgtgt gacaataaga gtatcggcag
     1561 gacattaaga ggaatgagcc atggcaaacc cggaacaact ggaagaacag cgtgaagaaa
     1621 cacgtttgat tattgaagaa ttactggaag atggcagcga tccggacgcg ttgtacacca
     1681 tcgaacacca tctttccgca gacgatctgg aaactctgga aaaagcggca gttgaagcgt
     1741 ttaaactcgg ttacgaagtg accgatccag aagagctgga agttgaagat ggtgatatcg
     1801 tgatttgctg cgacatcctc agcgagtgcg cgttgaatgc cgatctgatc gatgcccagg
     1861 ttgaacaact gatgacgctg gcggagaaat ttgacgttga gtacgacggt tggggcactt
     1921 actttgaaga tcctaacggc gaagatggcg acgatgaaga ttttgtcgat gaagacgatg
     1981 acggggttcg ccactaatta attgattaaa atgcccgata gcacatacta gcgggcattt
     2041 ttttagagtt cgcgtagcat ccgcacttca caatcgacat ggcccgtgca gccaagcgca
     2101 tagtcgatat gctcaaagcc caaatgctca taaagcgcaa tggcttcctt taaaaaagcg
     2161 gtcgtttcca gatagcagcg tttgaagccc atctctcgtg cctgctccat cgccattaag
     2221 gccagttttt tcgctagccc tttaccgcga atagccggaa gaaaatacat cttttgcagt
     2281 tcgcaaatgt ccgactcact cccggttaat ggcgcaatcc caccgccgcc gaccacttca
     2341 ccctcgtact caaccaccca atatgcatgg ccaggctgac tatacacttg atacagctcg
     2401 tcaagattcg gatcggcgac ggtgtagcct ttatcagcgg taagaccgta ttcggcggat
     2461 acctgacgaa tgacgcgggc gatagcagga ttatccagca gcgtcaggcg acgcattaca
     2521 ggtgattgcg gcgcaatgtt attcatagtt taa
//
LOCUS       DAZOXR010000331         2519 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZOXR010000331 DAZOXR010000000
VERSION     DAZOXR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.338"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(819..1370)
                     /locus_tag="ACHUNN_004419"
     CDS             complement(819..1370)
                     /locus_tag="ACHUNN_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0578598.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            2169..2465
                     /locus_tag="ACHUNN_004420"
     CDS             2169..2465
                     /locus_tag="ACHUNN_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0578599.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          719 a          534 c          483 g          783 t
ORIGIN      
        1 acaaaatggt aagattgttc acatgacctt acttaacaga cttttaagag actttgaaac
       61 agcctgttta acaggtcact acctctggtc ttatcgttcc cttctttcta ggtggttatc
      121 tgttaagagt ttttcagttt ttattcttaa gataattgtt aagatgatac tctcccttcg
      181 ggttatatcg tatctcttct cccgttaaat atcccttcac agtcagacta aataacacct
      241 gtgtagttct tggtagaatc tttcaaggta acttagctct gagtttatga agttgaccta
      301 tgagatagac tctaccgaat ctgttagtca attgttgctt aggtgctgct aaggcttact
      361 tagtggaaag agaggggata tgtgaccata ggggaacccc tcctgttaaa agactactta
      421 gaagtctgag gttttagtaa aatctgtgaa tatggcttct cagacgctct gtaacgcatt
      481 ctaagcgatt ttaccccttt attttaaggt ggttagtaca tccctatagg ttacccctaa
      541 aaatggggtt aaaactcgct acagagcttt ctatgagatt agcttctaat cgaatcaact
      601 atgaggtttg gtaggattgt gcaaaatctg tacagtaact tttcagaatc tgaaaatttt
      661 tataaaaaat gactagccct ctttgaatct catcttcccc gaatcttcaa aaatatctga
      721 cagatgtctg tagctgctaa gtacccgtca caccagctat gtgaccccct tggcaaaaaa
      781 gccaaatatc agctacatcc atgtaactat tcagtaactt atttagcaag tctgtaataa
      841 gttgctctag agattcctaa ctcttcctga atctgcaacc tcgtcttacc ttgtgaaata
      901 agcgtctgta cagcctctga agaagctttt acaggcctcc ccatatgtac cccttcagct
      961 ttagcacgtt caataccagc ctttctacgc tctgctatca agcttctttc aagctgtgcc
     1021 aacgttgaca taatgcctaa cattgcttca cccatagcgc ttgataagtc aaacccttct
     1081 ctaaggctga taactgtcac acctttagcc tgtagcgctt taaccgttga taacacatca
     1141 attgtgtcac gtccaagacg gtctacagct accacaacta cggtgtcacc ttctcgtgca
     1201 taagctaata gtctgctgaa accgtttctt aaagaagctt tgacagaacc actgacagct
     1261 tcatcagtga accatttagc aatgttgtac cctgcctctt caatttgcat ttgctggtta
     1321 gtgatggatt ggtcatctgt actcaccctg caatatccaa gtatcgccat aatgttcccc
     1381 ttagaagtct caaaagttag tctcataagt ttagctgtct cataatccaa aaacaacctt
     1441 ttgagactac ttgagggtgc attctaccgt tacgttgaaa gtgtctcaaa aggaagtcct
     1501 tttaagactg gttatgaagg agctatgagg ttgattaggg gttggtacaa gcccaactaa
     1561 ccgaatcaac tatgaagatt ttaggagggt aactgtaacc atcactttct aactttcacg
     1621 taggaaattg ttaagtggtt tgatagttac accatgtgac caagcatcta tatatcgtta
     1681 tatagccctg tatatagcac tatatgtagt gtcgagagag gaacgactaa cacgatacag
     1741 tttaccctga aaagagactt cacagattct ttatatattt caataagtta gggtaaaaag
     1801 ggactatacc gagactgtat agaatctgtg tagaattgca tagatacttg gaaatctact
     1861 attaaattgc atagggactc acctgatatt gtcctgtttt taacaggtct cctagaaaac
     1921 cgaatagact tcattcaagg gtcattgaag tcataatcat ttcagtagaa aaaccaagtc
     1981 tcgattttaa atgcaaatag caatcatatt caataaggca agacgatcag gatcagtgaa
     2041 aatgtcattt ttggcgtaat tgaccttact ttatgcatgg tcttaacaaa gacatcatct
     2101 ttccgttgca gatcttcgcc ctttcaaata acgtacttta caactttccc gaacaaggag
     2161 ttgtgcccgt gttgtatgtt atttacgctc aagacaaagc tgatagcctc gaaaaacgcc
     2221 tttccgttcg tccggcacat ttagcacgtt tacagttact gcatgatgaa ggtcgtttgt
     2281 tgacagctgg tccaatgcca gcggtagaca gtaacgatcc aggggctgcg ggatttactg
     2341 gctcaaccgt tattgctgaa tttgaatcgc tggaagcagc tcaggcctgg gccgatgctg
     2401 acccgtacgt agcggcaggt gtctatgaac acgtttcagt gaaaccattt aagaaagtgt
     2461 tctgaataga gattcaatat ttaagggcac ggtttttgca accgtgttct tgtttggcc
//
LOCUS       DAZOXR010000332         2472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZOXR010000332 DAZOXR010000000
VERSION     DAZOXR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.339"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..713)
                     /locus_tag="ACHUNN_004421"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="ACHUNN_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="ACHUNN_004422"
     CDS             complement(766..1362)
                     /locus_tag="ACHUNN_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0578600.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="ACHUNN_004423"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="ACHUNN_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR0578601.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2472)
                     /locus_tag="ACHUNN_004424"
     CDS             complement(2006..>2472)
                     /locus_tag="ACHUNN_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0578602.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          679 a          537 c          496 g          760 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagcg gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 aggttgtcgc tt
//
LOCUS       DAZOXR010000333         2447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZOXR010000333 DAZOXR010000000
VERSION     DAZOXR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.340"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(26..316)
                     /locus_tag="ACHUNN_004425"
     CDS             complement(26..316)
                     /locus_tag="ACHUNN_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0578603.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHUNN_004426"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHUNN_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0578604.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="ACHUNN_004427"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="ACHUNN_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0578605.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHUNN_004428"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHUNN_004428"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..>2447)
                     /locus_tag="ACHUNN_004429"
     CDS             complement(2172..>2447)
                     /locus_tag="ACHUNN_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0578606.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          576 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgacc
//
LOCUS       DAZOXR010000334         2440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZOXR010000334 DAZOXR010000000
VERSION     DAZOXR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.341"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            49..1002
                     /locus_tag="ACHUNN_004430"
     CDS             49..1002
                     /locus_tag="ACHUNN_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0578607.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1043..2038)
                     /gene="pgl"
                     /locus_tag="ACHUNN_004431"
     CDS             complement(1043..2038)
                     /gene="pgl"
                     /locus_tag="ACHUNN_004431"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0578608.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2334..2429)
                     /locus_tag="ACHUNN_004432"
                     /pseudo
     CDS             complement(2334..2429)
                     /locus_tag="ACHUNN_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          663 a          615 c          560 g          602 t
ORIGIN      
        1 ttaacggcca gccacccaaa atcgtcgctt ccattttccg gagtaataat gaagcatgaa
       61 ttatcaagta tgaaggcatt tgtcatactg gcagagtcca gttcgtttaa caatgccgct
      121 aaattactca atattacgca acctgcatta acgcgaagaa taaaaaagat ggaggaagat
      181 ttacatattc aactatttga gcgtacaact cgcaaggtta cgttaacaaa agcaggaaaa
      241 aggttgctcc cggaggcgcg ggaattaata aagaaattcg atgaaacgct ttttaatatt
      301 cgtgatatga atgcttatca tcgtggtatg gtgacgttag cgtgtattcc gaccgcagta
      361 ttttattttt taccgctggc aatcggtaaa tttaatgagc tgtatcccaa tattaaagtg
      421 cggattctgg aacaaggcac gaataattgc atggaatcgg tgctgtgtaa cgaatcagac
      481 tttggcataa acatgaacaa cgtcacaaat tcatccattg attttacccc actggtcaac
      541 gagccgttcg tgctggcctg tcggcgtgac catccattgg ccaaaaagca gcttgtagaa
      601 tggcaggagt tagtgggtta caaaatgatt ggcgtacgct cctccagcgg caaccgactg
      661 ttgatagaac aacagctggc cgacaagccc tggaagctgg actggtttta cgaagtgcgt
      721 catctttcga cgtcattagg actggtagag gcgggactgg ggatttcggc gcttcctggt
      781 ctggcaatgc cccatgcgcc ctattcttcg attattggta ttccactggt ggaacccgtt
      841 atacgtcgga cattggggat tattcgccgc aaagatgccg tgctttctcc ggcagcagaa
      901 cgcttttttg ccttgctgat taatttgtgg actgacgata aagacaattt gtggaccaat
      961 atcgtcgaac gccagagaca tgcgttacag gagataggtt aatacaccgg cgcaggagag
     1021 actgcgccgg gtaaatcagc ggttagtgtg cgttaaccac cacccacatt ggtccctgcc
     1081 cgaccgcata gcggcctttt tcatgcagta gcccctgctc gccaacaatt tcgtataccg
     1141 agatgtggtg agatttttgc ccggcgacaa tcagatactt gccgctgtga tcaacattga
     1201 agccgcgcgg ctgggtttcc gttggctgga agccttcttt actcaacacg ctgccatctt
     1261 ccgaaacgct gaaaacggta atcagactgg cggtacggtc gcaggcgtat aaatgacgac
     1321 catccggggt gatatgaata tcagccgccc aacaggtgtc ggagaagttt tccggcatca
     1381 tatccagcgt ctggacacat tcaatattgc cgtgtggatc tttcagttcc cagacatcca
     1441 ctgagctgtt taactcattg acgcaatacg catattgttc gtttggatgg aataccatat
     1501 gacgcgggcc ggccccttca acggtagtca cttccgcagg gtcctgcgcc accagatgac
     1561 catcatcgct gaccgtaaac aggcaaatgc gatcctgctt taatgccgga acccacagcg
     1621 tacggttgtc cggtgagata ttggcggaat ggcaaccgtc cagcccctcg accacatcga
     1681 cgacgcccac tggcaggcca tcttccagac gcgttacgct cacgttaccc gcattgtaag
     1741 aacctacaaa gacaaactgc ccctggtgat cggtggaaat atgcgtcgga ctacccggca
     1801 gcgcagactc tgcggcaaag gtcagtgcgc catcgtccgg ggcaatacga tacgccagga
     1861 cgcgaaactc agggcgaaca ccaacataga gataacgttt gtccgggctg accaccatcg
     1921 gctgcacctg ccccggcaca tcgacaacct gtgtcagcgt cagtgcgcct tcatgattca
     1981 ggttccagac gtgaatttgc tggctctcag ggctggcgat ataaactgtt tgcttcatga
     2041 acacacctca aaaatgataa atcccttatc tttcatacac ataacaacac ttactggata
     2101 aaaaacagac aacaaaacac gataaaatac aatatcgcac atcaattaaa tcaataagtt
     2161 agcactccat ttcgaatggc atattactaa aatattagtc accgacacga atttgacaat
     2221 gccacagata ttttcgcgac tattcaggga gtatgactct ccccgctttt gtttacctgt
     2281 atcaacgacc gtaaacacta tgccataaca acttctggcc aacttcccat tcgctcaaca
     2341 atcaactcac ttccggcaca aataaacgca ttacgttgta gatcggtatt ctggtcattt
     2401 gttgatacgc gtacatagcc aataagcatg gtaaatcccc
//
LOCUS       DAZOXR010000335         2437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZOXR010000335 DAZOXR010000000
VERSION     DAZOXR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.342"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            221..337
                     /locus_tag="ACHUNN_004433"
                     /pseudo
     CDS             221..337
                     /locus_tag="ACHUNN_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(442..518)
                     /locus_tag="ACHUNN_004434"
     tRNA            complement(442..518)
                     /locus_tag="ACHUNN_004434"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:tct)
     gene            complement(532..608)
                     /locus_tag="ACHUNN_004435"
     tRNA            complement(532..608)
                     /locus_tag="ACHUNN_004435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Arg,seq:tcg)
     gene            complement(616..691)
                     /locus_tag="ACHUNN_004436"
     tRNA            complement(616..691)
                     /locus_tag="ACHUNN_004436"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Ile,seq:cat)
     gene            complement(732..1790)
                     /locus_tag="ACHUNN_004437"
     CDS             complement(732..1790)
                     /locus_tag="ACHUNN_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR0578609.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1898..2138)
                     /locus_tag="ACHUNN_004438"
                     /pseudo
     CDS             complement(1898..2138)
                     /locus_tag="ACHUNN_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2363..>2437)
                     /locus_tag="ACHUNN_004439"
     CDS             complement(2363..>2437)
                     /locus_tag="ACHUNN_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0578610.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          585 a          698 c          560 g          594 t
ORIGIN      
        1 tagcggtcat gtgatgccgg tttcccggta actcagcacc ggtatctgag tcaacgtttt
       61 ctctactggg tcatttccga tacgccctgc ctgctgacag gctttcatca catctgaaaa
      121 tatagcaccc tgactgatac tgtagtacct aaggttccag aaactgtgat gtatccggca
      181 cagaaaagcc cctccggaga gggctggaga gtggcgctat gtgccattgc atgatgccgg
      241 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      301 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      361 caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaaag gcccgcaaaa
      421 gcgagccagg gaaaataagt gtggcgcgtt gtactggatt cgaaccagtg accgattgct
      481 tagaaggcaa ttgctctgtc cggctgagct aacaacgcat gatgcagata atggaccgcc
      541 atcggggact tgaaccccgc gcagccagct tcgaaggctg gcgctctttc ctgatgagct
      601 aatggcggta tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
      661 tcgctcgctc tcaccactga gctaaagggc cgggagccgc ataataacga cgcgtaatta
      721 attcttcaat atcatccgtt ctggctgact aaatcctgta cttcccgaac cgtctgctca
      781 aaacgttcag tctccagctc aacgccaatt gcacgacgcc cgagcgccag tgccgctttc
      841 actgtcgaac ccaaccccat gaaaaaatct gcaatcaggt cacccggacg actgctagcg
      901 ctgattatct gctgcagcat ttctgccggt ttttcgcacg gatgtttccc gggatagaac
      961 tgcaccggtt tatgcgtcca cacatcggta tacggcacct gcaccgtcac gccaaaatac
     1021 cgccgcagat gtttatattc actcagcagc tccgcatact gccggttcag tgaggtatac
     1081 gtatccacca gctggtggtg gagcttttcc agttcaccac gccgatgctt ctcttctgcc
     1141 acccgggcaa acagcgcctg taatttcaga taatcgcttt cattcggcaa ctgccactga
     1201 ccggcactga accagtgcga caccatgttt ttctttcctg tggcatctgc aatctgtttt
     1261 gccgttatcc ccagggcagc tcgcgcatca cgaaagtaag aaatcagcgg ggccatcaca
     1321 tgctgtttca gtgcactgcc cttcgccgca tacccggcat ctttcggacg atacggcccc
     1381 tgataatgtt ccgcgaacag aatgcgctct gtggcgggga aatacgcccg caggctttcc
     1441 ttgttgcatc cgttccagcg tccggacggc ttcgcccaga taatatggtt cagcacactg
     1501 aagcgttcac gcatcatgat ttcgatatca gatgccaggc gatgaccaca gaacaggtaa
     1561 agacttccgg caggtttcag cacccgccag aactgcgcca gacactggtc cagccacttc
     1621 aggtaatcat cgtcgccctt ccactggtta tcccagccct caggcttcac tttaaagtac
     1681 ggcgggtccg tgactatcag gtcaacagaa ttttcgggta acgaccggat aaattccagg
     1741 cagtcggcgt tgattaactc acaactggat atttttacag tattaagcat ggatcattaa
     1801 gcctgtctct gataggctca ttctgctttt gcgcaaagca gtgggcctga ggtttgcttg
     1861 tgaacccaac gcatgagcag atggctggtg ggtgccccta acacccacca gccgcccatt
     1921 taccacaaat aaaaaagcct tcactgcgga aggcgtctgt aacaaccgaa ctgatagtct
     1981 gccagacccg ccataaccag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa
     2041 gtattctgcg ctatctcccc gactgtcgcc ggttcggtaa cgcttaattc attaaacacc
     2101 actctggcgg tttctgtcat atcctgctgt tttagcatgt cttttccttt tccggttaac
     2161 gtgacacacc aataactctt gtcgaaaaag ccagcaagct gaaagacagg tattcaccgc
     2221 caccagcgcg tttactgtac tgacgcgatt tcagtcataa aaaacccgct cggcggcggg
     2281 ttgtagaaac tcttctaacg tcaggcataa aacgcccatc gttatgacga atttaccaca
     2341 gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc tcagcccatg
     2401 cttcttcaat atcaaaccgg gtcaccagcg catcata
//
LOCUS       DAZOXR010000336         2433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZOXR010000336 DAZOXR010000000
VERSION     DAZOXR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.343"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUNN_004440"
     CDS             complement(<1..304)
                     /locus_tag="ACHUNN_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0578611.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(343..630)
                     /locus_tag="ACHUNN_004441"
     CDS             complement(343..630)
                     /locus_tag="ACHUNN_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578612.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(880..1035)
                     /gene="hokD"
                     /locus_tag="ACHUNN_004442"
     CDS             complement(880..1035)
                     /gene="hokD"
                     /locus_tag="ACHUNN_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0578613.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1263..1928)
                     /locus_tag="ACHUNN_004443"
     CDS             complement(1263..1928)
                     /locus_tag="ACHUNN_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0578614.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2097..>2433)
                     /locus_tag="ACHUNN_004444"
     CDS             complement(2097..>2433)
                     /locus_tag="ACHUNN_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578615.1"
                     /translation="ELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQ
                     VRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARI
                     RRATKSRTE"
BASE COUNT          610 a          628 c          539 g          656 t
ORIGIN      
        1 gctctgcggc ttcgctcaga gctggcgtat gttcccggcc tactgattca cacaggtaat
       61 caacgaaacc ggacaccagt tttcgttcgc gaggcgtgat cgccccaccg accggagtcc
      121 agtaatcgaa acccagttgc aggagtttga aaaaacgctt gtggaatgcg tagttacaca
      181 cacgcttaaa gtctgcgtgt atccactcac ctattttgat ttgatgcaga aaatcgcaac
      241 tctccggcgt cgccgggaga agtaatccgg aagaggtttg tttgaccagt tgtatatgtg
      301 ccatcggctt tctccggtgg cacggtgtta cacagcagga gttcaatcct gctcaagatt
      361 gtagatgagt ttattcttca gcaaaagcag aaaaccagcc ttaaaaccaa tctctttcaa
      421 aacccgtaat gatgtgacaa attcgtcctc acgcaaaata aaaccgtccg tcagaagtcc
      481 attacaaaaa taaaataaca cagcaccgct cttcctttgt tgagattgca aacatctaat
      541 gcggcaatgg ctgacaatcg ctccattctc aacgcgcaca gcatagaggc cattttcact
      601 aaaaatttca cgcaattctt cgattttcat cttcagaatc cttccagata aatagctctc
      661 ccctgttcgg ggtccatccc tcttctccct gcgcgctact taagtgagtc gattctatct
      721 gcgaaggtgc gcgaatcaaa ttcaccggaa ataaacaaca aaaaacccgc cgaaacgctg
      781 agagatcaca gcgatgggcg ggttaagtgc gggtgcgttg aggatgcctg acacatcaga
      841 ggtgggcggg gatgggatca gctccccgcc cggtcactct tacttcctgg attcgtagtc
      901 tacgaagaca gcgacctccg tctggccggt tcggattcgt acctcacaga ggtctttcct
      961 cgttaccagt gccgtcacta tgacggttaa acagatgacg atcagggcga ttaacatcgc
     1021 cttttgctgc ttcatagcct gcttctcctt gcctttcggc atgtaagagg ctaacctaca
     1081 tgtgcaaagc atgaaattgg cctcagatta atgttaagcg tcttgccgga cgcgtaatgt
     1141 taactggggc ttttctctat ctgccttttg gtgttcatgc ctgaagcaga tagcctcaag
     1201 cacccgcagt cattctactt acctcgccaa tatgaaatca atcagaaagg tgccccataa
     1261 aatcactcct tctcttcttt accgtagtgg agttgaccaa ttttgataag agggcgtccc
     1321 tgagatttgc ggtgtagatt ggtatcgcgc agagaataca cacagccaca atattcctgc
     1381 tgatagaatt tttcgcgctt gctgatttca atcatacggg acgagccgcc ctgcttgcgc
     1441 cagttataat cccagtacac catgcccgga taatgcgcga cggctcgccg cccacagtcg
     1501 ttaacctgct gcatattttt ccagcgtgaa atgcccagtg aactgctgat cacactgaaa
     1561 ccattttcag cggcgtacag cgctgtccgc tcaaaacgca tgtcaaaaca catggtgcaa
     1621 cggatccctc gttcgggctc ccattccatt cctttggctc gttcaaacca gttgtctgtg
     1681 tcgtaatcag catcaataaa cggcacgccg tgttgttcag caaagcgaat attctcatcc
     1741 ttacgaatta aatactcttt ctgaggatga atgttcgggt tgtagaaaaa gatggtgtag
     1801 tcgattcccg aggcctgaag cgcctccatc acctcaccgg aacatggagc acagcaagag
     1861 tgcagtagta gtttgtttgc cccgtttggg agctccaatt tgggccgttt gaaatcagca
     1921 actgtcataa atatgtttat tggggtcata aaaatatcaa agagtgtagc attagaacgg
     1981 ggctatcgga aacagatgtg tgactgctcc ccgccctttc gggtggtctc ctgatgattt
     2041 gagggtgcag aaatccctcc ggttaaggat taaattttta acagtgctaa atttaattat
     2101 tcagttctgg attttgtcgc cctgcgtatc cgcgctttcg cgttacgctc aatctgtatc
     2161 agcttttcta tatttctccg cctttcctgt tcctcctggc gcaatagcct tacatcatct
     2221 gccagtctgg tttctctttt tgccacagag agcatccagt caaacggctc cacaaccgca
     2281 ccgcagattt tacagcggac ctgacgctct ttttcgtcaa cccggacaga ggcgtgatgg
     2341 caatatggtc tttccgatgg ttcataaaga aaattaacct gattacgtgg gtcatcctct
     2401 tttaccggaa ataaaacgat attgcttaac tca
//
LOCUS       DAZOXR010000337         2413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZOXR010000337 DAZOXR010000000
VERSION     DAZOXR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.344"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            267..410
                     /locus_tag="ACHUNN_004445"
     CDS             267..410
                     /locus_tag="ACHUNN_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0578616.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            423..632
                     /gene="cspE"
                     /locus_tag="ACHUNN_004446"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             423..632
                     /gene="cspE"
                     /locus_tag="ACHUNN_004446"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0578617.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            798..1607
                     /gene="rlmA"
                     /locus_tag="ACHUNN_004447"
     CDS             798..1607
                     /gene="rlmA"
                     /locus_tag="ACHUNN_004447"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0578618.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1604..2170)
                     /gene="mntP"
                     /locus_tag="ACHUNN_004448"
     CDS             complement(1604..2170)
                     /gene="mntP"
                     /locus_tag="ACHUNN_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0578619.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          633 a          580 c          592 g          608 t
ORIGIN      
        1 cactatgtaa taacttctcc cactggcctg gaacaactga acttattgaa ctatgttaga
       61 aaatacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg atatatcggc
      121 gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct
      181 gatcgagaca tgtttaaaaa tggcttgcca taattaacgt tgtatgtgat aacagatttc
      241 gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa gtcctgagta
      301 aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc tcatgcagta
      361 atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc
      421 aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt tttggcttca
      481 ttactccggc tgatggcagc aaagacgtgt tcgtacactt ctccgctatc cagggtaatg
      541 gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac ggccagaaag
      601 gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga agtgtgaata
      661 cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg ctttatcttc
      721 gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc ggcctttagt
      781 atcctgccgg agttgtcatg tctttttcct gtccactttg ccatcagcct ctttcgcgtg
      841 aaaaaaacag ctatatctgt ccccagcgac atcagtttga tatggcgaaa gaagggtatg
      901 tcaatctgct gtccgttcag cataaacggt ctcgtgatcc gggcgacagc gcggaaatga
      961 tgcaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt gatgcaattg
     1021 tcgcccaact gagggaacgg cttgatgata aggccacggc ggtgctggat attggctgtg
     1081 gtgaagggta ttacacacac gcatttgccg atgcgttgcc cgaaatcacc acgtttggtc
     1141 tggatgtttc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag gtcacttttt
     1201 gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc ataatacgta
     1261 tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc ggctgggtca
     1321 ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt tacaatgaag
     1381 tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag cagagtgcgg
     1441 agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg cagatgacgc
     1501 cgtttacctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa gaagtgttcg
     1561 actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg tggaagtgcg
     1621 tccagaggat ctggacgccg atgccgatca gtaccagccc gccgagaatt tctgcttttt
     1681 tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgttgac ataatcaagg
     1741 ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgcaggaaa gcaagaccaa
     1801 cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt agccagaaac
     1861 cgtgtcgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc tcaataatca
     1921 ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtggttccat tcaaggacaa
     1981 accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt tcgacggcac
     2041 caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg gtggcacctt
     2101 taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga acagtagcag
     2161 tgatattcat gacaatgtcc tgaccggggt atccataaaa cacatcatcg cccccagcaa
     2221 acatgcatac atgaacaaca agccgtgatg gttttcgcgt tcatgcaggc atagcgattg
     2281 atgtgcatat ggtctcgcct gactgaaagt attcagtccg tacgtgccac gttttgcaac
     2341 gagtatgttg acacgtacat ttccggaatc tggaaatcgg ctactcccca aatgagggcg
     2401 caaccttaac ata
//
LOCUS       DAZOXR010000338         2402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZOXR010000338 DAZOXR010000000
VERSION     DAZOXR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.345"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            75..164
                     /locus_tag="ACHUNN_004449"
                     /pseudo
     CDS             75..164
                     /locus_tag="ACHUNN_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="ACHUNN_004450"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="ACHUNN_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFR0578620.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="ACHUNN_004451"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="ACHUNN_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="ACHUNN_004452"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="ACHUNN_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          666 a          505 c          618 g          613 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 ct
//
LOCUS       DAZOXR010000339         2330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZOXR010000339 DAZOXR010000000
VERSION     DAZOXR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.346"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            20..238
                     /locus_tag="ACHUNN_004453"
     CDS             20..238
                     /locus_tag="ACHUNN_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0578621.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="ACHUNN_004454"
     CDS             240..605
                     /locus_tag="ACHUNN_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0578622.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="ACHUNN_004455"
     CDS             602..1267
                     /locus_tag="ACHUNN_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578623.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="ACHUNN_004456"
     CDS             1267..1632
                     /locus_tag="ACHUNN_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0578624.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="ACHUNN_004457"
     CDS             2087..2242
                     /locus_tag="ACHUNN_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0578625.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          496 c          628 g          579 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggttt
//
LOCUS       DAZOXR010000340         2317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZOXR010000340 DAZOXR010000000
VERSION     DAZOXR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.347"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..452)
                     /locus_tag="ACHUNN_004458"
     CDS             complement(<1..452)
                     /locus_tag="ACHUNN_004458"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0578626.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALT"
     gene            554..778
                     /locus_tag="ACHUNN_004459"
     CDS             554..778
                     /locus_tag="ACHUNN_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0578627.1"
                     /translation="MQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERSLL
                     EAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            890..1153
                     /locus_tag="ACHUNN_004460"
                     /pseudo
     CDS             890..1153
                     /locus_tag="ACHUNN_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(1190..1747)
                     /locus_tag="ACHUNN_004461"
     CDS             complement(1190..1747)
                     /locus_tag="ACHUNN_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0578628.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1744..2019)
                     /locus_tag="ACHUNN_004462"
     CDS             complement(1744..2019)
                     /locus_tag="ACHUNN_004462"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR0578629.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            2204..2317
                     /locus_tag="ACHUNN_004463"
                     /pseudo
     CDS             2204..2317
                     /locus_tag="ACHUNN_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          655 a          478 c          549 g          635 t
ORIGIN      
        1 tcagttaagg cctgcgccgc attgcgtatt acacagcttt ttgtagatat atttccaggc
       61 aggcagtgaa gaaggtaaaa catggaaatg gaatcaaatt gaccatgtaa cgccgcggga
      121 taaggttcaa aaacatcatg gctaatttta tgtttaattt ttgattcccc agcccttgta
      181 gatgccgcgt tcaggctagc ttcgttcaaa tccattaaag atatcagact actctcaggt
      241 acgtgagtaa ggtaaaaccc agttccaaca ccaatatcca gatggttgtt acctaaatgt
      301 tccagaaagt gtggaagaag gtgttccttt gtaggacatc cccatgcaag ccgatttgat
      361 actcccaaaa cccaccagtc ataaagcttt agggtaagtg gtgtgtaaat tttagcccca
      421 tcatctgtgt ttgttttcat tgatttcacc atgttatagt tttatttgtg aattaaatca
      481 attatggcga tgaattacaa ggggttaaat gctgccgcag catagcgata ttgaaatagc
      541 ctagtatgct tcgatacagc aggagccgaa tggctggaag accgtcacca cacagttcta
      601 catccaggaa tttagtgagt atattgcgcc actgcaggat gctgtagatc tggaaatcgc
      661 aacggaggaa gaaagatcgt tgctggaggc atggaataaa tatcgggtat tgttgaatcg
      721 tgttgataca tcaactgcac ctgatattga gtggcctgca aatcctgtca gggagtaatc
      781 attgggatta tgccgcagac acgtcgtatg caggaacgtg ctgcggttag tttgtgagct
      841 ttcgatagtg gttgttattt ttgcccttat ttgttccgga ggccatggtt caatggtccg
      901 tctgccccct gtggtgatgt cagcaaaatc agccactgcg cgaaccacaa tagcccggga
      961 agatgctgaa gatcaccagg taaagctgtc agcgcagaaa ctggaagaac tgctcgcatc
     1021 aatggttaag gatgaggttg atcgcaatga tgggatttat tgacgtcagc gagagcagaa
     1081 ggaagaactg aataacctga atgatttacg ctcaatcaga gcgatggcga ttagcggcaa
     1141 tcacgctcga taaaattaac ctcggtgatc atccggggtt ttgcattcat taaaaccgca
     1201 tcaacctttc caccaattgt tctttacggg caacgatcca cccatgttgc tccagataaa
     1261 atttaaaccg ttccagagtg cataccatcg catcggcggg gactttttct gtgaactcga
     1321 cctgaccgtg tttatcgaag tggatcagta atgcgcatcc attatttggg gcgggggagt
     1381 tttgtgctgc aggaggacgt ttgtggagtt cttcttccat tgcgttaaat ttttcaatgt
     1441 aagcttcttt tagtctggct gcttttttgc cagtaaatcc cattacgagg aacatgaagc
     1501 cgtcttttgt gatcaggtag catcgtgatt cacgttctgc gccgttgcca atttcgacag
     1561 tctgaacatc ggccgaaaag ttggctgatg taaattcggg agagcagtcg agtgtgtcaa
     1621 tcttagctaa aacgtgttta tgttgtttat cgaagtagtc ggcaacagct tgagaggtgg
     1681 taaccaggcg accatctttg aaaaagactt ttggtgactc gttagaaaaa gttatagctg
     1741 tattcatatg tgatgactcc aattaattgg atgatcacca cctctgacgc caatcaggga
     1801 ggtggtgaga cgtacagggt tggcgtaacc ggtcatcaca aaacccggcg cggatttctc
     1861 cgcccctgca cgccccacca taatgcgaat gtggcaatgc ttaacgcaaa aaaaaccgca
     1921 aacgcggcta tgcgttgtga tgttatccga gacgccaatc ccgacaaccg attttgcggt
     1981 tgtgtgagga atatagcccc ggagaacaaa aactgtcaac gggcaaaaaa atttccgtta
     2041 tcaggaatgt ataaaattaa agcgatcgag caaaaaatcc tttctgcaaa actaaaaaag
     2101 atttcaataa aatcaggcag ttaaaaacac ggaaaacggt ataaaaaagc gcctcaaaat
     2161 cctgtttttt ctttcagata caatgtctta ttgcattttt taaaacatgt cagtgtggta
     2221 catggatatc gataccatcg ccaattataa actgggcggt gcacagcaac atgatagcgt
     2281 acgcctcgat ccgtgggtgt ttatgttctc agcagga
//
LOCUS       DAZOXR010000341         2262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZOXR010000341 DAZOXR010000000
VERSION     DAZOXR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.348"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..425)
                     /locus_tag="ACHUNN_004464"
     CDS             complement(<1..425)
                     /locus_tag="ACHUNN_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFR0578630.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNR"
     gene            complement(502..729)
                     /locus_tag="ACHUNN_004465"
     CDS             complement(502..729)
                     /locus_tag="ACHUNN_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578631.1"
                     /translation="MTRRGRNAACGQCPASGRARDGRFYRSRLALVTVYASPSPFSDE
                     RPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            630..836
                     /locus_tag="ACHUNN_004466"
     CDS             630..836
                     /locus_tag="ACHUNN_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578632.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRSCRAASAGAVKRGARRH
                     LLLPAKQNPKTTHTQPNKTPKQDK"
     gene            942..1904
                     /gene="parM"
                     /locus_tag="ACHUNN_004467"
                     /gene_synonym="stbA"
     CDS             942..1904
                     /gene="parM"
                     /locus_tag="ACHUNN_004467"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR0578633.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            1907..2257
                     /locus_tag="ACHUNN_004468"
     CDS             1907..2257
                     /locus_tag="ACHUNN_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0578634.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
BASE COUNT          707 a          529 c          515 g          511 t
ORIGIN      
        1 cgattgcccg aatatttcca gcccagcacg tccggcagcg ggttttgtgg cagacgtgga
       61 cggccttttg ccaggatgta ggcacattcg tggcgatagc ccacatatgc ggccttcgat
      121 gtgtagtttt tggtgaagac caggtgacca acaacgctga atcccgcatt tttccaggcg
      181 gccataaagc gatcgacgcg gttccagcca tagaagctca ccattaacgc gtcttttttg
      241 agtacgcggt acatttcatt acaggctggt tgcagccatt catcggtttt atcgccggcg
      301 atggtgcgcc cggaacggtc acggaaaccg acgagatacg gcgggtcggt gaggatgaaa
      361 tcgacagcat tgcccggaaa cgtggccata acacgcacgc agtcacccag gataaaacgg
      421 gacataacag cactccttct cttacagagg gcaccgggag ggcacccctg tggtgcccgt
      481 tccccgctgc ctgtgggctg ttcaccgcgt ccggtaacgg gtcaggccga ccgtggaata
      541 ccgcagccgt cgtctttttg acggcgagga tatgccgcga aagcggcttg aagggcgttc
      601 atcagaaaag ggcgacgggc tggcgtacac tgtcaccaac gccagccggg agcggtaaaa
      661 gcgcccgtcc cgtgcccggc cggacgcggg acactgacca caggcagcgt tcctgccgcg
      721 ccgcgtcagc cggcgcagta aaaagaggag cccggcgaca tcttttgtta cccgccaaac
      781 aaaacccaaa aacaacccat acccaaccca ataaaacacc aaaacaagac aaataatcat
      841 tgattgatgg ttgaaatggg gtaaacttga caaacaaacc cacttaaaac ccaaaacata
      901 cccaaacaca caccaaaaaa acaccataag gagttttata aatgttggta ttcattgatg
      961 acggttcaac aaacatcaaa ctacagtggc aggaaagcga cggaacaatt aaacagcaca
     1021 ttagcccgaa cagcttcaaa cgcgagtggg cagtctcttt tggtgataaa aaggtcttta
     1081 actacacact gaacggcgaa cagtattcat ttgatccaat cagcccggat gctgtagtca
     1141 caaccaatat cgcatggcaa tacagcgacg ttaatgtcgt tgcagtgcat cacgccttac
     1201 tgaccagtgg tctgccggta agcgaagtgg atattgtttg cacacttcct ctgacagagt
     1261 attacgacag aaataaccaa cccaatacgg aaaatattga gcgtaagaaa gcaaacttcc
     1321 ggaaaaaaat tacattaaat ggcggggata cattcacaat aaaagatgta aaagtcatgc
     1381 ctgaatctat accggcaggt tatgaagttc tacaagaact ggatgagtta gattctttat
     1441 taattataga tctcgggggc accacattag atatttctca ggtaatgggg aaattatcgg
     1501 ggatcagtaa aatatacgga gactcatctc ttggtgtctc tctggttaca tctgcagtaa
     1561 aagatgccct ttctcttgcg agaacaaaag gaagtagcta tcttgctgac gatataatca
     1621 ttcacagaaa agataataac tatctgaagc aacgaattaa tgatgagaac aaaatatcaa
     1681 tagtcaccga agcaatgaat gaagcacttc gtaaacttga gcaacgtgta ttaaatacgc
     1741 tcaatgaatt ttctggttat actcatgtta tggttatagg cggtggcgca gaattaatat
     1801 gcgatgcagt aaaaaaacac acacagattc gtgatgaacg ttttttcaaa accaataact
     1861 ctcaatatga tttagttaac ggtatgtatc tcataggtaa ttaatgatgg acaagcgcag
     1921 aaccattgcc ttcaaactaa atccagatgt aaatcaaaca gataaaattg tttgtgatac
     1981 actggacagt atcccgcaag gggaacgaag ccgccttaac cgggccgcac tgacggcagg
     2041 tctggcctta tacagacaag atccccggac ccctttcctt ttatgtgagc tgctgacgaa
     2101 agaaaccaca ttttcagata tcgtgaatat attgagatcg ctatttccaa aagagatggc
     2161 cgattttaat tcttcaatag tcactcaatc ctcttcacaa caagagcaaa aaagtgatga
     2221 agagaccaaa aaaaatgcga tgaagctaat aaattaattc aa
//
LOCUS       DAZOXR010000342         2233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZOXR010000342 DAZOXR010000000
VERSION     DAZOXR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.349"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..76)
                     /locus_tag="ACHUNN_004469"
     tRNA            complement(1..76)
                     /locus_tag="ACHUNN_004469"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="ACHUNN_004470"
     tRNA            complement(79..155)
                     /locus_tag="ACHUNN_004470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="ACHUNN_004471"
     tRNA            complement(157..233)
                     /locus_tag="ACHUNN_004471"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
     gene            complement(423..977)
                     /locus_tag="ACHUNN_004472"
     CDS             complement(423..977)
                     /locus_tag="ACHUNN_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0578635.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(974..1264)
                     /locus_tag="ACHUNN_004473"
     CDS             complement(974..1264)
                     /locus_tag="ACHUNN_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0578636.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1264..1863)
                     /locus_tag="ACHUNN_004474"
     CDS             complement(1264..1863)
                     /locus_tag="ACHUNN_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0578637.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1935..2147)
                     /locus_tag="ACHUNN_004475"
     CDS             complement(1935..2147)
                     /locus_tag="ACHUNN_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578638.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          553 a          593 c          471 g          616 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      301 cctgtgccgt ttttcaggta taaaaaaacc cgcataaagc gggctctttc aaatgtccat
      361 gtctgctatt cgcctcgcgg tacagctttg cgaagcgtac aagaattgaa gcagttttta
      421 cgtcaaaaag caataacttt tttctctata ccaaaagcca taaccattgg tttgtacaaa
      481 ataaattctg ccacctttag ccaatgctca atgcgtcttt cacaggttct taaactccat
      541 ttcgggtgtg catcattcag cagttcagcc attttgcgtt tggtcatccc ccgcccctca
      601 taccgttgcc gaaggacgct aatcaatcct ggatgctctg ccagcacctc acttatgacc
      661 cgatcaatac ataacgcctc tgcatcagta caatgcgcca gccagctctt ttgcttaccg
      721 ttaatcatat cccgcaaaaa agcctcaagt tcagacttgt tcagacctgc ttttttcatc
      781 ctccggagcg cctcgttaat tgccgttttt gtcagctttt tagaggtcaa catcaggtta
      841 aacatattcc ccgtcttacc gccaccaata tacgaccagc gcccccacat acgtagtttt
      901 ccctgaatcc agacactttc cagcgtggtg agacgaaggt gttctccgct ttttcctgta
      961 ttcgttgggt aaatcataaa tatccctcct ttctccagat ttcttgtgtg cgaaaaacac
     1021 cttctgcatg catcaggcgt aattcttctt tggtgtaatc gctggttttt acccgcccgt
     1081 cgattaaatc gtggcacgag ctacaggcaa tcgctgcctg catatcgtgt ggctttatcg
     1141 ctgttccgca cgttcccgcc agtcggtaat gcgccagcac agacgtttcc ggatcgtgat
     1201 tgcagtagcc aggaattctg acggtgcaca tctgcccccg cgccgcttta cgtaaatcca
     1261 ccattacgca aactccagca gctgcgcggc cacattttcg acttgttccg gagaggaaaa
     1321 tttacggaac agaatccagt tccacagcac attcagaaca gatttataaa cctgctgaaa
     1381 ctcggtttcg tccatattcg caaaagcgat ggattttgcc cgacgcccgc ggctaccgtc
     1441 cggataaaaa tgttcggtgt aaaatccagc ctgaatggtt acccactcgc gaaaagcctc
     1501 aaacgacttt agcaatgccg tatcccgggt tctgcgtgtc gcaactgtat tcagatattg
     1561 ctctgcggct tcgctcagag ctggcgtatg ttcccgacct actgattcgc acaggtaatc
     1621 aacgaaacca gacagcagtt ctcgttcgcg aggcgtgatc gccccaccga ccggagtcca
     1681 gtaatcgaat cccagttgca ggagtttgaa aaaacgcttg tggaatgcgt agttacgcac
     1741 acgcttaaag tctgcgtgta tccactcacc tgttttgatt tgatgcagaa aatcgcaact
     1801 ctccggcgtc gccgggagaa gtaatccgga agaggtttgt ttgatcagtt gtatatgcgc
     1861 catcgtagtt ctccgctggc gcagtagaat gggtgttcag cccgttatgt agtataccag
     1921 aattaatgcc aatactaaca ggatgctctg actcgcaatt catccagcag tttatcattt
     1981 cccataatgt cacttaccct catcggtaaa aaaattgcct ttcgaccatt acgatacatc
     2041 attgattttt tggtttcagg gaagtaatcc atttcgacta taactgacag gtcatcacga
     2101 cgtatgactg cgtatttact actaaatagt ttctttattt tttccacgat gcctccaggt
     2161 ttataagtac aaacggttat atcaacatag agacaaaagg tgatgctgcc aacttactga
     2221 tttagtgtat gat
//
LOCUS       DAZOXR010000343         2196 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZOXR010000343 DAZOXR010000000
VERSION     DAZOXR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.350"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..257)
                     /locus_tag="ACHUNN_004476"
     CDS             complement(<1..257)
                     /locus_tag="ACHUNN_004476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578639.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQVFHWLIYSH"
     gene            194..1693
                     /gene="pitB"
                     /locus_tag="ACHUNN_004477"
     CDS             194..1693
                     /gene="pitB"
                     /locus_tag="ACHUNN_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0578640.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1742..>2196)
                     /locus_tag="ACHUNN_004478"
     CDS             complement(1742..>2196)
                     /locus_tag="ACHUNN_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFR0578641.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT          518 a          528 c          573 g          577 t
ORIGIN      
        1 atatgtgaat atattaacca gtggaatacc tgtgttttga ttatttctaa aggttttgaa
       61 tgaatgctta ttgtctgata cacaagaaat aacactcttt ttatcgttaa aaaatgatat
      121 ttcactttgc ccatgccgtt aaagtcactg ataatgcgtc cgttcgtaaa ttcaaaatgg
      181 cgtaatctaa tatatgctaa atttatttgt tggccttgat atatacacag ggcttttgtt
      241 attgcttgct ctggcatttg tgttgttcta cgaagcaatc aatggttttc atgacacggc
      301 gaatgcggtg gcaaccgtta tttatactcg tgccatgcaa ccacaacttg ctgtggtgat
      361 ggcggcattt tttaactttt ttggcgtgtt attgggcgga cttagcgttg cctatgccat
      421 tgtccatatg ttgccaaccg atttgttgct gaatatgggg tcaacccacg gcctggcgat
      481 ggtcttttcc atgctgctgg cggcgattat ctggaacctg ggaacgtggt tcttcggttt
      541 accggcctcc agttcgcaca ccttgattgg tgcgattatc ggcatcggtt taaccaacgc
      601 gctgttaacc ggctcatcgg tgatggatgc gttaaacctg cgtgaagtga ccaaaatttt
      661 ctcctcgctg attgtttccc ctatcgtcgg cctggtcatt gcgggaggcc tgatattcct
      721 gctgcgacgc tactggagcg ggacgaaaaa gcgtgaccgt attcaccgca ttccggaaga
      781 tcgcaaaaag aaaaaaggca aacgtaaacc gccattctgg acgcgtattg cgctgattgt
      841 ttccgctgcg ggcgtggcgt tttcgcacgg cgcgaacgac ggacaaaaag ggatcggcct
      901 ggtgatgctg gtactggtgg ggattgcccc tgctggcttc gtcgtcaata tgaacgcgtc
      961 cggctatgaa attacccgta cccgcgatgc cgtcaccaac ttcgaacact acctgcaaca
     1021 gcatcctgaa ctgccgcaga agttgattgc gatggaacct ccattgcctg cagcatcgac
     1081 tgatggcacg caagtaacag agtttcactg tcatccggca aatacctttg atgcgattgc
     1141 gcgcgttaaa acgatgctgc caggcaatat ggaaagttac gagccgttaa gcgtgagtca
     1201 gcgcagccag ctgcgccgca ttatgctgtg catctctgat acctccgcga agctggcgaa
     1261 actgccaggc gtcagtaaag aagaccagaa cctgctgaaa aaactgcgca gcgatatgtt
     1321 aagcaccatt gagtacgctc cggtgtggat catcatggcg gtagcactgg cgctcggcat
     1381 tggcaccatg attggctggc gtcgtgtagc gatgaccatc ggtgagaaga ttggtaagcg
     1441 cggcatgacg tatgcgcaag gcatggcggc acaaatgacg gcggcagtgt ctatcggcct
     1501 tgccagttat attgggatgc ctgtctccac aacacacgtc ctctcgtctg cggtagcagg
     1561 gacgatggtg gtggacggcg gtgggttaca gcgtaaaacg gtaaccagca tcctgatggc
     1621 gtgggtgttt actttaccgg cggcaatttt tctttctggt gggctgtact ggatagcatt
     1681 gcagttgatt taaccttcct gaaaatgccc ggtcctggcg atcgggcatt tccatttttg
     1741 actagtgata accacgcgcg gtcataaaat ccgtaatcgc tttttctgca tcaaccaaca
     1801 cctgttccag tggctgattg gcatcgatat caaccagttg tgcaccacca aaggttaact
     1861 gtggcgttat ggcaatcttc ctcgccagcg attcccgttt atggtcgggt ttacgtgcac
     1921 aggcaacttc aaggtcaaca ttgagtttga tgaccagatc aggcttatgg ctcgccatcc
     1981 agtgaaacgc tttacgttcc tggcttgcca gccatgagac aaaacgacca ccttcaacgt
     2041 taggtgggaa caccgtacca tcgtaagcgc caggaatttg gtcctgagga taacggtcgg
     2101 ttagaacaat taacccgcga cgacgacagg caagcatatg acgaaagcgc agtaagcgac
     2161 gggcgacaaa cgctgtaatt accagcgccg gaactg
//
LOCUS       DAZOXR010000344         2191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZOXR010000344 DAZOXR010000000
VERSION     DAZOXR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.351"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(263..463)
                     /locus_tag="ACHUNN_004479"
     CDS             complement(263..463)
                     /locus_tag="ACHUNN_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578642.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="ACHUNN_004480"
     CDS             complement(463..735)
                     /locus_tag="ACHUNN_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0578643.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="ACHUNN_004481"
     CDS             complement(738..1016)
                     /locus_tag="ACHUNN_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578644.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="ACHUNN_004482"
     CDS             complement(1017..1574)
                     /locus_tag="ACHUNN_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578645.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          595 a          446 c          421 g          729 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc t
//
LOCUS       DAZOXR010000345         2181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZOXR010000345 DAZOXR010000000
VERSION     DAZOXR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.352"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            150..659
                     /locus_tag="ACHUNN_004483"
     CDS             150..659
                     /locus_tag="ACHUNN_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0578646.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            656..2074
                     /gene="phrB"
                     /locus_tag="ACHUNN_004484"
                     /pseudo
     CDS             656..2074
                     /gene="phrB"
                     /locus_tag="ACHUNN_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
BASE COUNT          533 a          537 c          580 g          531 t
ORIGIN      
        1 aatgcagctc gggaaatagt agaataattc tcactcagat gaagttgata atatttttac
       61 caacaaaata cttccccgca aaaactctgc cccataaaat tcatttacca tcgtctacac
      121 ttactccttt ataccgttca ggagcagtaa tgaatctaca acgatttgat gacagcaccc
      181 taatccgtat ctttgccctt catgagttac atcgactgaa agaacatggc ttaacgcgcg
      241 gggcgcttct cgattatcac agccgctata aactcgtctt tctggcgcat tctcagccgg
      301 agtaccgcaa acttggcccg ttcgtggctg atattcacca gtggcaaaat ctggatgact
      361 attacaacca gtaccgccaa cgcgtagttg ttttgctttc tcaccccgcc aacccgcgcg
      421 atcacaccaa tgttttgatg cacgttcagg gttattttcg cccgcatatt gattccacag
      481 aacgccagca gctggctgcg cttatcgaca gttatcgccg tggcgagcaa ccacttcttg
      541 cgccgctgat gcgtatcaaa cactatatgg cgctttatca tgacgcctgg ctttcagggc
      601 agcgttattt cgaactttgg ccgcgtgtga ttaacttgcg ccattcagga gttttatgac
      661 tacccatctg gtctggtttc gccaagattt acgtctgcac gataatctcg cactggctgc
      721 cgcctgccgc aattcgtctg cacgcgtgct ggcgttgtat atcgctacac cacgccagtg
      781 ggcgacgcat aacatgtcgc cgcgtcaggc tgaacttatc aatgctcaac tgaatgggct
      841 acaaatagcg cttgcggaaa aaggtattcc tttattgttc cgtgaagtgg atgactttgt
      901 cgccagtgtc gaaatagtta aacaggtgtg cgcggaaaac agcgttaccc acctgtttta
      961 taactatcag tatgaagtga atgagcgggc gcgggatgtg gaagttgaaa gagcgctgcg
     1021 taacgtggtg tgtgaaggat ttgatgacag cgtgatcctg ccgcctggcg cggtgatgac
     1081 cggtaatcac gagatgtaca aagtctttac gccttttaag aatgcctggc tgaaacggct
     1141 gcgggaaggg atgccggagt gcgtcgctgc gccaaaagtt cgtagtagcg gatcgataga
     1201 gccctcgcca tccattacgc tgaattatcc tcgtcagtct ttcgatactg cgcattttcc
     1261 ggtggaagaa aaagcggcga ttgcgcaatt acgccagttt tgccagaacg gtgccggaga
     1321 atatgagcaa caacgagatt ttccggcagt ggaaggcacc agccgtttgt cggccagcct
     1381 ggcaacgggc gggttatcgc ctcgccagtg cttgcatcgc ttgttggctg aacagccgca
     1441 ggcgctggac ggtggggtcg gtagtgtctg gcttaatgag ctgatctggc gcgagtttta
     1501 ccgtcacctg ataacgtatc acccctcgtt gtgtaaacat cgtccaatta ttgcctagac
     1561 ggatcgtgta cagtggcaga gcaatcccgc acatttacag gcctggcagg aaggcaaaac
     1621 gggatacccg attgttgatg ccgctatgcg tcagcttaac agcactggct ggatgcataa
     1681 caggctacgg atgattacag ccagttttct ggtgaaagat ttattgatcg actggcgcga
     1741 aggcgagcga tatttcatgt cgcagctgat tgatggtgat ttggcagcca ataacggtgg
     1801 ctggcagtgg gccgcttcaa ccggaaccga tgcagcgccg tattttcgta ttttcaaccc
     1861 gacaacccag ggcgagaaat ttgatctcga aggtgagttt atccgccagt ggctaccgga
     1921 actacgcaat gtaccgggga aatcggtgca tgagccgtgg aagtgggcgc agaaagcagg
     1981 tgtgaagctg gattatccgc aaccgatagt cgagcacaaa gaggcgagag tacaaacgtt
     2041 ggcagcgtat gaggcggcgc ggaaggggaa ataacagacg cgtcaggcaa tcgagcccag
     2101 atgccggatg cggcgtgaac gccttatcca gcctacaaat ttgtgcaaat tcaataaatt
     2161 gcaggaaata cgtaggcctg a
//
LOCUS       DAZOXR010000346         2113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZOXR010000346 DAZOXR010000000
VERSION     DAZOXR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.353"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            190..1167
                     /gene="intI2"
                     /locus_tag="ACHUNN_004485"
                     /pseudo
     CDS             190..1167
                     /gene="intI2"
                     /locus_tag="ACHUNN_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(1355..1594)
                     /locus_tag="ACHUNN_004486"
     CDS             complement(1355..1594)
                     /locus_tag="ACHUNN_004486"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0578647.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          611 a          461 c          442 g          599 t
ORIGIN      
        1 atgccgtcta tcctgcttgc acgatgcaca ttatctcacc caaagaactc ataacatttt
       61 aatttattta gcattaaatt aaaactctac cggtaaacaa gcatctctag gcgtaaaaaa
      121 caggcacttt tttaaatccc agctagaata ggctgtatag gcagacagtt gcaagacaag
      181 gagcgtttta tgtctaacag tccattttta aattctatac gcacggatat gcgacaaaaa
      241 ggttatgcgc tgaaaactga aaaaacttac ctgcactgga ttaagcgttt tattctgttt
      301 cacaaaaaac gtcatcctca gaccatgggc agtgaagagg tcaggctgtt tttatccagc
      361 ttagcaaaca gcagacatgt agccataaac acgcagaaaa tcgctttaaa tgccctagct
      421 tttttgtaca acaggttttt acaacagccg ttgggcgata ttgattatat ccctgcaagc
      481 aagcctagac ggctaccctc tgttatctct gcaaatgaag tgcaacgcat tttgcaggtt
      541 atggatactc gcaaccaagt tatttttacg ctgctgtatg gtgcaggttt gcgcattaat
      601 gaatgcttgc gtttgcgggt taaagatttt gattttgata atggctgcat cactgtgcat
      661 gacggtaagg gtgggaaaag cagaaacagc ctactgccca cgcgcctaat cccagcaata
      721 aaataactca ttgagcaagc gcggcttatt cagcaagacg acaacttaca aggcgtaggg
      781 ccatcgctgc cttttgcttt agatcacaaa tacccttctg cttatcgaca agcggcgtgg
      841 atgtttgtct ttccctccag cacgctctgc aaccacccgt ataacggcaa attatgccgc
      901 catcatctgc atgactccgt tgcgcgaaag gcattgaagg cagccgtaca aaaagcaggc
      961 atcgttagca agcgtgtcac ttgtcataca tttcgtcact cgtttgctac gcatctatta
     1021 caagcggggc gtgatattcg cactgtgcaa gaactcttag ggcataacga tgttaagacc
     1081 acgcaaatct atacgcatgt gttgggtcag cattttgccg gcaccaccag tcctgcggat
     1141 ggactgatgc tacttatcaa tcagtaaaag tagataaata tctttatgta gccctgagta
     1201 gcccctgata ctttttatgg tgaaaaagat aaacaatcat agaggggata aatatgagcc
     1261 agttctacca ttgttcagat aagtttaagc aagatactgt caatcacagt gaccattcat
     1321 ggtcattaca cttcctccag ttgccagaca atgcctaaca taaaaacgca aaagcccaaa
     1381 agtcagagct taggtgttct aggcgttttc taagcgctga agaaatacgt cttaccgtag
     1441 attgcgtgcg tttctgaagc acattttcct tatacatggc tttatcccag gcagcttcat
     1501 caagctcttg gagtaacaag ccagtaataa tgcgacactc tcgggtttgt agcgagccgc
     1561 tggtgagatc ggagttatag cggaaagggg tcatttgtaa ttatttgtgg gtggaggata
     1621 gttaacttat tattgtagtt ggaagaggct gctttccaaa cattagcacc tcgtaatgct
     1681 tgttactgta tattaattta tgctcttagc gcatgttaat gggctattac aatgcataat
     1741 gtccacatca agataactgt ggcttaacac ctgtacaagc gaaaaaaatc aatcacctgt
     1801 ccggaaatac gggatcactg taaagtgttg gtgaggcagt ctaggagggg gatttaattt
     1861 gggatactga tgaaaggatt atgatcagtt ggatatagga tcgtactaag ctgagcggca
     1921 ggttagctag attagttaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     1981 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     2041 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     2101 acagggctgc cga
//
LOCUS       DAZOXR010000347         2098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZOXR010000347 DAZOXR010000000
VERSION     DAZOXR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.354"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..88
                     /locus_tag="ACHUNN_004487"
     CDS             <1..88
                     /locus_tag="ACHUNN_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0578648.1"
                     /translation="GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            104..418
                     /locus_tag="ACHUNN_004488"
                     /pseudo
     CDS             104..418
                     /locus_tag="ACHUNN_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            520..1974
                     /gene="amn"
                     /locus_tag="ACHUNN_004489"
     CDS             520..1974
                     /gene="amn"
                     /locus_tag="ACHUNN_004489"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0578649.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          511 a          515 c          500 g          572 t
ORIGIN      
        1 gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca
       61 ttatctgaac ataaaacact atcaataagt tagagtcatt accatggcgc ttgaagcaca
      121 atctattttc tggatagttt tcttctccat aatgctggca aacattgcgc atgacatggt
      181 ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt gccagttatc gctatagtgg
      241 cgctggagtc ggttatcagg ttgccagtgt ggttggcggt ggatttacac cttttattgc
      301 cgctgcactc atcacttact ttgccgggaa ctggcatagc gtcgccattt atttgctggc
      361 tggatgcctg atttccgcaa tgaccgcttt gttgatgaaa gacagtcaac gcgcttgata
      421 gcctggcgaa gatcatccga tcttcgcctt acacttttgt ttcacatttc tgtgacatac
      481 tatcggatgt gcggtaattg tatggaacag gagacacaca tgaataataa gggctccggt
      541 ctgaccccag ctcaggcact ggataaactc gacgcgctgt atgagcaatc tgtagtcgca
      601 ttacgcaacg ccattggcaa ctatattaca agtggcgaat tacctgatga aaacgcccgc
      661 aaacaaggtc tttttgtcta tccatcactg accgtaacct gggacggtag cacaaccaat
      721 ccccccaaaa cgcgcgcatt tggtcgtttt actcacgcag gcagctacac caccacgatt
      781 actcgcccta ctctctttcg ttcgtatctt aatgaacaac ttacgttgct gtatcaggat
      841 tatggtgcgc atatctctgt gcaaccctcg cagcatgaaa tcccttatcc ttatgtcatc
      901 gatggctctg aattgacact tgatcgctca atgagcgctg ggttaactcg ctacttcccg
      961 acaacagaac tggcgcaaat tggcgatgaa actgcagacg gcatttatca tccaacggaa
     1021 ttctccccgc tatcgcattt tgatgcgcgc cgcgtcgatt tttccctcgc acggttgcgc
     1081 cattataccg gtacgccagt tgaacatttt cagccgttcg tcttgtttac caactacaca
     1141 cgttatgtgg atgaattcgt tcgttgggga tgcagccaga tcctcgatcc tgatagtcct
     1201 tacattgccc tttcttgtgc tggcgggaac tggatcaccg ccgaaaccga agcgccagaa
     1261 gaagccattt ccgaccttgc atggaaaaaa caccagatgc cagcatggaa tttaattacc
     1321 gccgatgctc agggtattac tctggtgaat attggcgtgg gaccatcaaa tgctaaaacc
     1381 atctgcgatc atctggcagt gctacgcccg gatgtctggt tgatgattgg tcactgtggc
     1441 ggattacgtg aaagtcaggc cattggcgat tatgtacttg cacacgctta tttacgcgat
     1501 gaccacgttc ttgatgcggt tctgccgccc gatattccta ttccgagcat tgctgaagtg
     1561 caacgtgcgc tttatgacgc caccaagctg gtgagcggca ggcccggtga ggaagtcaaa
     1621 cagcggctac gtactggtac tgtggtaacc acagatgaca ggaactggga attacgttac
     1681 tcagcttctg cacttcgttt taacttaagc cgggccgtag caattgatat ggaaagtgca
     1741 accattgccg cgcaaggata tcgtttccgc gtgccatacg ggacactact gtgtgtttca
     1801 gataaaccat tacatggtga gattaaactt cccggtcagg ctaaccgttt ttatgaaggc
     1861 gctatttccg aacatctgca aattggcatt cgggcgatcg atttgctgcg cgcagaaggc
     1921 gaccgactgc attcgcgtaa attacgaacc tttaatgagc cgccgttccg ataataagtt
     1981 ctgaaccttc ttatcagaaa tagatgtgaa ggagtaagta agacacctgg caaatagcct
     2041 gcaataatcg tgggctgttt gcttccttgg gcggatacga gttttattat cgtcttaa
//
LOCUS       DAZOXR010000348         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZOXR010000348 DAZOXR010000000
VERSION     DAZOXR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.355"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUNN_004490"
     CDS             complement(60..1025)
                     /locus_tag="ACHUNN_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0578650.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUNN_004491"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUNN_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578651.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZOXR010000349         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZOXR010000349 DAZOXR010000000
VERSION     DAZOXR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.356"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHUNN_004492"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHUNN_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR0578652.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHUNN_004493"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHUNN_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR0578653.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZOXR010000350         2074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZOXR010000350 DAZOXR010000000
VERSION     DAZOXR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.357"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            3..699
                     /locus_tag="ACHUNN_004494"
                     /pseudo
     CDS             3..699
                     /locus_tag="ACHUNN_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1452
                     /locus_tag="ACHUNN_004495"
     CDS             706..1452
                     /locus_tag="ACHUNN_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0578654.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1467..1889
                     /locus_tag="ACHUNN_004496"
     CDS             1467..1889
                     /locus_tag="ACHUNN_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0578655.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1890..>2074
                     /locus_tag="ACHUNN_004497"
     CDS             1890..>2074
                     /locus_tag="ACHUNN_004497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578656.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          568 a          456 c          604 g          446 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagagga agaggaagag gaagaggaag atctaaatac
      361 tgatctcccc ctcgccaaaa acgagcgtct aaaaaattcg agccggaggc tattgagctg
      421 cccgattggt tgccggaaac actctggcat gagtgggtcc ggttcagaca ggcattgcga
      481 aaaccgattc gaacggagca gggcgctaac ggggcgatac gggaactgga aaaattccgt
      541 cagcagggtt ttacacctga gcaggtgatt cgacacagca tcgccaatga ataccagggc
      601 ctgttcgcgc cgaaaggtgt tcggcctgag acgttgctcc gacaggttaa caccgtctcg
      661 ttgccggaca gtgcgatccc gccaggcttc agggggtaac ggaccatgaa aaatattgcg
      721 acaggcggcg ttctggaacg catccgccga ctgaccccgc cacatgtaac cgccccattc
      781 agaacggtag cggagtggcg cgagtggcaa cttgctgaag gccagaaacg tagcgaggag
      841 atcaaccgcc tgaatcgcca gttgcgggtg gaaaaaattc tgaatcgctc aggcatccag
      901 ccgttgcacc gtaaatgctc gtttgcgaat taccaggtgc agaacgacgg ccagcgatac
      961 gcgttaagcc aggcgaaatc catcgccgat gaactgatga ccgggtgtac aaattttgcg
     1021 ttcagcggaa aacctggtac cgggaagaat cacttagcgg cagctatcgg gaatcgcctg
     1081 ctgaaagacg gtcagacagt gattgtggtt accgtggctg atgttatgag cgccctgcac
     1141 gccagctatg acgacgggca gtcaggcgaa aaatttttgc gggagctgtg cgaagtggat
     1201 ctgctggttc ttgatgaaat tggcattcag cgcgagacga aaaacgagca ggtggtgctg
     1261 caccagattg ttgatcgccg gacagcgtcg atgcgcagcg tggggatgct gacaaacctg
     1321 aactatgagg tgatgaaaac attgctcggc gagcgggtga tggatcgcat ggtcatgaac
     1381 ggcgggcgct gggtgaattt taactgggag agctggcgtc cgaatgttag ccattcgagg
     1441 gttgttaagt agtttcagga ggatttatgg cgaaaccttt tactcccgaa cagcgggaag
     1501 aactgaagac gcgaattgtg gaactcgtgc atcaggacgg tcgggtcacg attcggcagt
     1561 tgtcagatga aacaggtatc agtcgtgcgt ctgtcggtcg cttatgcata gaactggtcg
     1621 caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag gctcgcaagg
     1681 actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg gttgttgttg
     1741 atccggacct tatctggtca ttacctgacg gagaaatacg ccgctacgac aggcgcctga
     1801 atataatctg tcgcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc
     1861 agggtaattt tcaggaggcg atactgtgaa tgaaattagc tatcaggctt caattaccgc
     1921 tggcattcgc atcaaaggag aggagcatgg aaataaaacc agaagatgag ttaagcaata
     1981 tcgttttatt tccggtaaaa gaggatgacc ctcgtaatca ggttaatttt ctttatgagc
     2041 catcggaaag accatattgc catcacgcct ctgt
//
LOCUS       DAZOXR010000351         2071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZOXR010000351 DAZOXR010000000
VERSION     DAZOXR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.358"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..115)
                     /locus_tag="ACHUNN_004498"
     CDS             complement(<1..115)
                     /locus_tag="ACHUNN_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0578657.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHUNN_004499"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHUNN_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0578658.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT          588 a          506 c          497 g          480 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 accgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacgggggtg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gaggggggtg
     1981 atggcttaac ggcgatatgt tctgctattt agcgttttta ctagtgagat agtccagttt
     2041 ctgaaaaata gccagtgtaa tgttttgtag g
//
LOCUS       DAZOXR010000352         2044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZOXR010000352 DAZOXR010000000
VERSION     DAZOXR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.359"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..854
                     /gene="espL1"
                     /locus_tag="ACHUNN_004500"
                     /pseudo
     CDS             1..854
                     /gene="espL1"
                     /locus_tag="ACHUNN_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1026..1133
                     /gene="yniD"
                     /locus_tag="ACHUNN_004501"
     CDS             1026..1133
                     /gene="yniD"
                     /locus_tag="ACHUNN_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0578659.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1186..1944)
                     /locus_tag="ACHUNN_004502"
     CDS             complement(1186..1944)
                     /locus_tag="ACHUNN_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0578660.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          634 a          457 c          416 g          537 t
ORIGIN      
        1 acgaatgcca tttgccccta tacatccatc ggcaatgttg ttaataatgg ccacaggcca
       61 gactcaatag cttattacac tattcaaaca gttgcccata ctccctgaaa aagaaatcat
      121 tgaaataata actgcgcaga atagcgttgg tacacctgct ttatttctgg ctatgatgaa
      181 cggacaaact gacaacgtaa aaatatttat gcaagaaatt cagtcactgg tatacaatca
      241 tatcattcat gaagataatc tggttaaatt actgcaaact aaaagtgcta acgaaacacc
      301 tggactctat atctccatgt tgtatggatt cgatgaaata atcgatatct ttctgaatgc
      361 attaaccact ccaatagcac aagaactttt aaacaaaaaa aatggtgatg agtattttag
      421 ccatgaaaat acatgatggt gagccaggat tatacgccgc aatggaaaat aatcaccctt
      481 tgtgtgtcac acggttcctc tctaaaatta atggcatcgc ctttaaatac aagttgagca
      541 aagctaacat catggattta ttaaaaggag ctacagcaca gggaacccct gcattataca
      601 tcgccatgag caagggtaat gaagacgtcg tgttatctta tatatcgacg ctgggtgctt
      661 ttgcaaaaaa acattctttt agtcaacatc agttatttac actattggcc gctaaaaatc
      721 atgacaacat gtcagctgtt catatagcca ttcatcataa gcattataaa actgtagaaa
      781 catattatgc tgctataaat gtcatcagcc aaagcctgag ttttagtgct gatgaaataa
      841 agacgtattt ataaccagct atttacgata tatatcgata cataatttta tttttttcat
      901 aaaacgcaga aacagtgacc atgtgcacaa cgccacccga cggctgtaat aacaaaaaat
      961 cccttgatgc ctatcccttt tgttacactc cgttatcacg cacaagagat atgcaggaca
     1021 ctggtatgcc gactaaacgc tttgataaaa aacactggaa gatggtggtg gtgctactgg
     1081 caatctgtgg cgctatgttg ttgctacgtt gggcagcaat gatttggggc tgagcagtga
     1141 caaacgaaga cagccagacc gcataacacg gtccggcaga taattttaca tgctgtagcc
     1201 cagcgataag gttgtccgac gatcggtatg ttccggtgca gactctggag gttcagagtt
     1261 ccatgtgacg ttgtaagcca ccttcagtcc aaaatgttca ttaatagcaa catttaatgc
     1321 gctttcggag ttcagcgttg tatcttccgc gccaaagacc gaaacaccct gcgtaaattt
     1381 agcgttgtca gtcaactgcc atgcataagc cccggaggca tagcccagcg gctgggtttc
     1441 actggcattg tcggtatatt tgtcgtaacg cacacctggg ccgaattcaa agcggaaact
     1501 gtgtaccggg ccattcaaaa actggcgacc ataacccgcg gtcaacacat cgcgctctcg
     1561 atagccgtta taacggtctg tcagccagct tgcctgacca aataaatagt cataatcagt
     1621 taaattaaaa cggctacgcc cgcccgccgc atatttttct gaagaacgct catcattaga
     1681 agaagtatta ctggcgttcc cccacagcga ccaggcagtg gtttgtccat accaggtcat
     1741 ggtggtgtca gccgtaagtg aggagctttt cgtattgcct gattgtgcaa gatatcctgc
     1801 gttcagatta ccttcgaaag gttttttagc gctggcagga tcgtccatga cagtaaaaac
     1861 ggaatcatcg gcagctgcat tcagtgacgc aaacatgccc cccgccaaca taacgatggc
     1921 aggaactgtc ttcaaaagct tcatttatca agagtccgta caacaaaaaa agagaccatc
     1981 gcggtcccgg aaactttctt aaggatcaaa gattagcgtc cctggaaagg taacgaatta
     2041 taaa
//
LOCUS       DAZOXR010000353         2022 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZOXR010000353 DAZOXR010000000
VERSION     DAZOXR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.360"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(46..282)
                     /locus_tag="ACHUNN_004503"
     CDS             complement(46..282)
                     /locus_tag="ACHUNN_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0578661.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            423..1211
                     /gene="fhuF"
                     /locus_tag="ACHUNN_004504"
     CDS             423..1211
                     /gene="fhuF"
                     /locus_tag="ACHUNN_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0578662.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1249..1926)
                     /gene="bglJ"
                     /locus_tag="ACHUNN_004505"
     CDS             complement(1249..1926)
                     /gene="bglJ"
                     /locus_tag="ACHUNN_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0578663.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1884..>2022)
                     /locus_tag="ACHUNN_004506"
     CDS             complement(1884..>2022)
                     /locus_tag="ACHUNN_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFR0578664.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          530 a          534 c          440 g          518 t
ORIGIN      
        1 cgcaccaatt atctttactt cctttcttgt ttcttccttg atttttcatc cctgattcaa
       61 attcattatc atcagcatga tggcagcaat aagcgccagg cacgatggta gcaaaacgta
      121 atgccgtaac tgtttgtgat acagcatcac cggcgtcaat actaatccca tcagaatgac
      181 aatccgccac tgatccggcg ataaatccgc atacatcagc cctgcaatca gcaatcccgg
      241 cagcaacact ccccagccac tgcccagcgt acgttgcaac atggtttcat ctctttcatt
      301 gataatgata accaatatca tatgataatt tttatcattt gcaagccaga gaaatccctt
      361 gctatcgggt aaacctatcg ctaggattag caatcattat catttagatt actatcccga
      421 ttatggccta tcgttccgca ccgctctatg aagatatcat ctggcgaacg catctccagc
      481 cgcaggatgc tgggcttgca caagccgtac gtgcgacgat cgccaaacat cgtgaacatt
      541 tgctcgagtt tatccgcctg gatgaacccg ctccgcttaa cgccatgacg ctggcgcaat
      601 ggtcatcacc gaatgcgcta agttctctac tcgcggtcta ttccgatcat atctatcgca
      661 accaaccaac gatgatccgc gagaacaaac cgctgatctc actatgggca caattgtata
      721 tcggcctgat ggtgccacca ttaatgctgg cgctactgac gcaggaaaag gcattagatg
      781 tgtcgccgga acatttccat gctgagtttc acgaaaccgg acgcgtcgcc tgtttctggg
      841 tcgatgtgtg tgaagataaa aacgcaacac cacattcgcc gcagcagcga atggaaacgt
      901 taatcagcca ggcgctggtt ccggttgtgc aggcactaga agcgaccgga gagatcaacg
      961 gcaaacttat ctggagtaat accggttatt tgatcaactg gtatctcact gagatgaagc
     1021 aactgctcgg cgaggcgacg gttgaatcgc tgcgccatgc cctctttttt gagaaaacgc
     1081 tcacgaacgg tgaagataat ccactctggc gtaccgtcgt gctgcgcgat ggcctgctgg
     1141 tgcgccgaac ctgctgccag cgttatcgct taccggatgt gcagcaatgt ggcgattgta
     1201 cgctgaaatg aaagcactgc cggggaagta aacccggcat catgcggatt aatagggatg
     1261 caacacatta cttgcttcgc aatgccgcat acataacaga atatctgcgg cctccaacaa
     1321 ccctgcatca gaactgacgc ccagtttcga catcacatta aatttatgtg cacggatcgt
     1381 tttgatattg cgtttaagct gctcggcaat ttgtgtcatt gagtagccac gcgacataaa
     1441 gcgcaatatt tctctctccg tcgggcttag cgtccggctt tggttaatgt accactgatt
     1501 gttcagtcgg tcggtcgcct gacgtacacc atttaatgac aaaaagagtt cctgatgaaa
     1561 aatctccagc gttgacgctt tacttaatac accgtccagc ggtgatggcg acaatgaacc
     1621 aatcagccga gcttcaatat catcatccgc aataactaaa cgccgggtac gcggaaactt
     1681 aatcgccagt tcagtcaggc aagataatcc ctctctgcgc tcacttctca tggcagaaaa
     1741 agaaaaaatg accgccgcaa atgagatccg cgacatggca tcctgaaatg actcctgact
     1801 ggtataggta tggagcttat aagggttacc cgcaaacttt ctgaataaac tctcaatacc
     1861 gatactgctc atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg
     1921 ttccattctg cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg
     1981 ctatttatcc atgcatacat ttcagcgttg ctgtgaattg cc
//
LOCUS       DAZOXR010000354         2010 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZOXR010000354 DAZOXR010000000
VERSION     DAZOXR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.361"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(177..1037)
                     /locus_tag="ACHUNN_004507"
     CDS             complement(177..1037)
                     /locus_tag="ACHUNN_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0578665.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1220..1651)
                     /locus_tag="ACHUNN_004508"
                     /pseudo
     CDS             complement(1220..1651)
                     /locus_tag="ACHUNN_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1717..>2010
                     /locus_tag="ACHUNN_004509"
     CDS             1717..>2010
                     /locus_tag="ACHUNN_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0578666.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT          500 a          517 c          465 g          528 t
ORIGIN      
        1 gccggacatc agcgctatct ctgctctcag gggtctgacg ctcagtggaa cgaaaactca
       61 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      481 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg cagcagtgtt atcactcatg
      661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      781 tgcccggcgt caacacggga taataccgca ccacatagca gaactttaaa agtgctcatc
      841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacga
     1021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta ccagggttat
     1081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1201 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg
     1261 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
     1321 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg
     1381 cttcctgtcg gccctcattc gtgcgttcta ggatcctccg gcgttcagcc tgtgccacag
     1441 ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc ccgtcatcaa
     1501 tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc atatcggcag
     1561 tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc tccaccttca
     1621 tcctcagcaa atccagccct tcccggtctg ttgggcactg ttgcaaatag tcggtggtga
     1681 taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa aggccggcat
     1741 tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac
     1801 cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac
     1861 cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac
     1921 ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg
     1981 gcgtatctgt accgggccgt cgacagccgg
//
LOCUS       DAZOXR010000355         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZOXR010000355 DAZOXR010000000
VERSION     DAZOXR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.362"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUNN_004510"
     CDS             <1..389
                     /locus_tag="ACHUNN_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578667.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUNN_004511"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUNN_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0578668.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUNN_004512"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUNN_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0578669.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHUNN_004513"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHUNN_004513"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHUNN_004514"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHUNN_004514"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZOXR010000356         2001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZOXR010000356 DAZOXR010000000
VERSION     DAZOXR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.363"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1517
                     /gene="tssI"
                     /locus_tag="ACHUNN_004515"
     CDS             <1..1517
                     /gene="tssI"
                     /locus_tag="ACHUNN_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0578670.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQ
                     TVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQV
                     GLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKD
                     GSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1593..>2001
                     /locus_tag="ACHUNN_004516"
     CDS             1593..>2001
                     /locus_tag="ACHUNN_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0578671.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSR"
BASE COUNT          495 a          501 c          627 g          378 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc
     1021 tggtggaaac ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg
     1081 tggggatcat tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta
     1141 tgaatacctc ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg
     1201 tgggaatggg ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga
     1261 tgaaggaaaa caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca
     1381 ttcatctgga aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg
     1441 gtgccacaca acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg
     1501 atatgcggca attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga
     1561 tgaaccatat taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc
     1621 agggcgacat gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag
     1681 gtgcgccgac gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg
     1741 tgaacccggt gctgggtgcg aaggtgcttc cgggcgagac ggaccttgcg ctgccctgcc
     1801 cgctgccgtt tattcttttc cgcgcctaca gcagctaccg gaccagaaca cccgcgccgg
     1861 tgggggtgtt tggtcccggc tggaaagcgc ccttcgatat ccgcttacag atacgcgatg
     1921 aaggcctgat actcaacgac agcggcggca ggagcattca ctttgagccg ctgtttcccg
     1981 gcgagataag ctacagccgc a
//
LOCUS       DAZOXR010000357         1939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZOXR010000357 DAZOXR010000000
VERSION     DAZOXR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.364"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUNN_004517"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUNN_004517"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR0578672.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUNN_004518"
     CDS             1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUNN_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0578673.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          510 a          451 c          456 g          522 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgt
//
LOCUS       DAZOXR010000358         1937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZOXR010000358 DAZOXR010000000
VERSION     DAZOXR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.365"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(50..184)
                     /locus_tag="ACHUNN_004519"
                     /pseudo
     CDS             complement(50..184)
                     /locus_tag="ACHUNN_004519"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1090)
                     /locus_tag="ACHUNN_004520"
     CDS             complement(197..1090)
                     /locus_tag="ACHUNN_004520"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0578674.1"
                     /translation="MSHIRPDFPEPYGLPVDGLRGKTSIFSHPVYLFLREFSLQDSRG
                     GSLSKKAESLSSGKITIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
     gene            1057..1937
                     /locus_tag="ACHUNN_004521"
                     /pseudo
     CDS             1057..1937
                     /locus_tag="ACHUNN_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="ACHUNN_004521"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          481 a          433 c          469 g          554 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tctcatcgtc tatggaaacg acattcgtgc tggatgcact
     1861 ggagcaggcg ttgtgggccc gtcgtccgtc tggcaccatc cattacagcg ataaaggctc
     1921 tcagtatgtg tcactgg
//
LOCUS       DAZOXR010000359         1922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZOXR010000359 DAZOXR010000000
VERSION     DAZOXR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.366"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..404
                     /gene="yqeJ"
                     /locus_tag="ACHUNN_004522"
     CDS             <1..404
                     /gene="yqeJ"
                     /locus_tag="ACHUNN_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR0578675.1"
                     /translation="AEKMIYNETCTANWVIFNDQGRANLTIDFMYNKKNKTGTVALSG
                     TWQQGNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIMDQVDDDRLAQLIPDFYVF
                     PEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(438..518)
                     /locus_tag="ACHUNN_004523"
     CDS             complement(438..518)
                     /locus_tag="ACHUNN_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578676.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(554..978)
                     /gene="yqeK"
                     /locus_tag="ACHUNN_004524"
                     /pseudo
     CDS             complement(554..978)
                     /gene="yqeK"
                     /locus_tag="ACHUNN_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1408..1898
                     /locus_tag="ACHUNN_004525"
                     /pseudo
     CDS             1408..1898
                     /locus_tag="ACHUNN_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          726 a          313 c          303 g          580 t
ORIGIN      
        1 ctgcagaaaa aatgatatat aacgaaacat gcactgcaaa ttgggtaatt tttaatgacc
       61 agggacgagc aaatcttacc atcgacttta tgtataacaa aaaaaataaa acaggaactg
      121 ttgcgttgag tggaacctgg caacaaggta acagagaaag taaatcaata cggcgaaata
      181 ttgaatacac atggattgaa aactatgaca cagcccattt aacatcaaag aaagttaata
      241 agtttgagat tatggatcaa gttgatgatg atagacttgc acaacttatt cctgattttt
      301 atgtctttcc agaaaaaagt gtaagctata atatactaaa gcaaggtaag catgctttta
      361 ttttgagcat tggtaacaga gcaataatgc attgtgcaag gtaaactaaa atagataaca
      421 aaaaagatgg aacctcgtta atcataaaac aataaaatta aatattctcg cagtatatgg
      481 cagtctaaag catcaaagtt ttgatcaaca tctttcattt tagacatctc cttgcaatgt
      541 aaaatatcat gtatcaacaa catctggtgc aaatccatga gtcgaactcg acattcatct
      601 tagctcgatt attattcctt cgtacagtcg atgtaaacaa tacagaaaga ggattattaa
      661 gaaccagttt actcaggaat caatagagga aaggactaac gtttctttaa aagaattgat
      721 ttcatcatct gttaaactaa actcatcatt gacagatcgt gagatataac tgtttttaac
      781 tttactcttt acgttgactt tattgacaga attaacattc acatatcttg aatttaatgt
      841 ccatgatgtt gtttgcgtga gaacattctc agcattaagg aattttttta acggaatcct
      901 tggtttcttt ttcgagtccg aatttacaat atcatgcata tagaccattt catgaatctg
      961 cgaaaactca atgtccattg tatacctcac atttttaccg tgactcgatg ttactgttca
     1021 ataatcacct tccatcaata ctaaaattaa tacccctaat gtgccgataa caaatatagt
     1081 cattctacgt aacgtctcca taaggtgata accttgatca aatgaatttc gaatataata
     1141 aaggaaatct tttgagatgt ttatcacgaa taaagctatt tatgttacca atatcatttc
     1201 gagtgcgcac actatctatc tgttttttcg agatataaaa aacaacgtgt aagttatgta
     1261 aattgccgat gctactgagt aacattatgg taaaaaaagt aaaaatgagc gatttttgat
     1321 agaataaagc atgagaaaag gtgatatttg acattatcag aagctgcgaa ttcggatttt
     1381 gctctaatca aatgagagaa atgtaaaatg aagccacgaa atattaataa tagcctacca
     1441 ctgcaaccat tagttcctga tcaggagaac aaaaataaga aaaatgaaga gaaatccgtt
     1501 aatccagtta aaatcacaat ggggtctggt ttaaattata ttgaacaaga atctcttgga
     1561 ggaaaatatc taacacatga tttgtcaata aagatagcgg atatttctga agagataatt
     1621 cagcaagcaa tattatctgc tatgagcata tataaatttt cgataacaga tgatttaatg
     1681 agtatggctg taaatgaact cataaaactg accaaaatag agaataatgt agacctgaat
     1741 aaattcacta ctatatgcac agacgttcta tcccccctcg tcaccagaca taataaagaa
     1801 aaaaaacaaa cgacattcta cccttctcaa aaatcccctt tttaattttc attgaaaaaa
     1861 tggcaattga tactatgaat ccaggtcata cacattaagg catttatgta atgtcgtaat
     1921 ta
//
LOCUS       DAZOXR010000360         1893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZOXR010000360 DAZOXR010000000
VERSION     DAZOXR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.367"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(166..1578)
                     /gene="pykF"
                     /locus_tag="ACHUNN_004526"
     CDS             complement(166..1578)
                     /gene="pykF"
                     /locus_tag="ACHUNN_004526"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0578677.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1586..1780)
                     /locus_tag="ACHUNN_004527"
     CDS             complement(1586..1780)
                     /locus_tag="ACHUNN_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578678.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          480 a          437 c          459 g          517 t
ORIGIN      
        1 taaaaaaact gagagaaaac aggatgcttc catcggattc atcttagata aagtgagatt
       61 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaagcg
      121 cccatcaggg cgcttcgata tacaaattaa ttcacaaaag caatattaca ggacgtgaac
      181 agatgcggtg ttagtagtgc cgctcggtac cagtgcacca gaaaccataa ctacgacgtc
      241 acctttgtgt gccagaccgc tctgcagagc cagttcttta cccagacggt agaaatcatc
      301 agtagaagtg atctctttaa ccagctgcgg cacaacgcct ttgctcagta ccaactgatg
      361 agccgttttt tcgttggtgg tcagtgccag tatggtggca tccgggaagt atttacgtac
      421 tgcgcgagca gatttaccgc cctgggtagc aaccacgatc agcggagcat ccagtttttc
      481 agcagtttca acggcaccac ggcataccgc ttcggtaatg cgcagtttac ggttgtcatt
      541 gttgaactcg agacggctgt tcatcacgcg gtcggtacgt tcgcagatgg tcgccatgat
      601 agaaaccgct tccagcgggt atttaccttt tgcggattca ccagacagca tcactgcgtc
      661 agtaccgtcg aggatggcgt ttgcaacgtc accggcttct gcgcgagtcg ggcgtgggtt
      721 tttgatcatg gaatccagca tctgggtcgc agtgataacg actttacgtg cacggataca
      781 tttttcgatc atcatcttct gggcgaagat aacttcttct accggaattt ctacacccag
      841 gtcgccacgc gcaaccatga tgccgtcaga ggcttcgagg atttcgtcga agttgttgag
      901 gccttcctgg ttttcgattt tggagatgat gtggatgttt tcgccgccgt gcgctttcag
      961 gtgctcacgg atttcgataa cgtcagaacg cttacgaata aaggaagcag caacaaagtc
     1021 tacgccttgt tcgcaaccaa agatcaggtc ctgtttgtct ttttcagcca gtgctggcag
     1081 ggcaatggaa acgccaggca ggttcacacc tttgttttcg cccaggtcgc cgttgttcag
     1141 cactttacag ataactttgt taccttcaat ggcagtaact tccataccga tcagaccatc
     1201 gtcaaccagt acggtgttgc caacagacag gtcagtagtg aaaccttcat acgttaccgc
     1261 aaccatttcg ctgttgccga taacagattt atcagtggtg aaagtaaagg tctgacccgc
     1321 tttcagagaa acgtcgttac cgccttccag tttcatggtg cggatttccg gacctttggt
     1381 atcaagcagg atagcggcgg ttttaccagt tttgctcatc acgttgcgca gattctgaat
     1441 gcgctgaccg tgttctgcat agtcaccatg agagaagttc agacgcataa cgttcatgcc
     1501 agcgtccagc attttagcta acatctcttc agattcggtt ttcggtccga tggtgcaaac
     1561 aattttggtc tttttcatga cagtcttagt ctttaagttg agaaggatgg gagaaacttg
     1621 ctttctgggc gcactgccgg aaagcgaatc cggtattacg aaagtggtgg tgccttgctc
     1681 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
     1741 gtgagtgcct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
     1801 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
     1861 aaaaaatata caacttgtta gacaataaat tgt
//
LOCUS       DAZOXR010000361         1879 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZOXR010000361 DAZOXR010000000
VERSION     DAZOXR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.368"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHUNN_004528"
     CDS             complement(<1..265)
                     /locus_tag="ACHUNN_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0578679.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEG"
     gene            complement(300..1040)
                     /locus_tag="ACHUNN_004529"
     CDS             complement(300..1040)
                     /locus_tag="ACHUNN_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0578680.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            complement(1145..1729)
                     /locus_tag="ACHUNN_004530"
     CDS             complement(1145..1729)
                     /locus_tag="ACHUNN_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0578681.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          549 a          395 c          384 g          551 t
ORIGIN      
        1 ttccttcaat ttcaataaat gtaatgcttt ggttaataat aggttgatcg ttgacataaa
       61 cactgacgtc gtacataccc ggtatagcag ggttaccttc actataccgg ctgagatcaa
      121 tatttgaggc attctgcccc atcaggaagg tatggtcata ttcaacaagt tcagcacaga
      181 aagcactatt gcaatacagc agcgcgcaaa acgtagcgat acgggtcaga tgattatgat
      241 aatttttagt tggttttata gtcacgttat gcttcctgcg gaattatatc cttgcctgat
      301 tacagactgg cattaccttc aatggcgcca ccaaaatcat taatggcgta aaaatgtact
      361 tttgcagaac ttgctttgcc attaaggcca gtgactttca tgacctcatc actaaatggt
      421 gcaatcattt tcacatcaat cggatagcgt ttaccgctag cttctaaatc accactgcta
      481 aaagagacgt agtaaggggt tggattggtc actcgcaatg acgccttacc ttctgaacct
      541 gaccagaacc acttcagggc taacggggct tcagagggat ttcccttcaa tccatccggg
      601 cgatagaaaa gttttatacg tgtgcgaaat gccagttgca gcagactttg attcgcgacc
      661 ttttctgcat ctggttttgg tggaacttcc agtacgttaa accagaacac gctctctctg
      721 tctttaggca gtgaggtgct ggctgtgtac attaatttga ttgtttgccc acgtttggca
      781 tcaatacgcg atactggcgg cgtggcagta aaagggactg taatactgcc aggctcagcg
      841 ttgttatcgc cagtatctaa ccaactctgg acaagcaacg ggttattccc tttattttcc
      901 agacgtacgt tgacactttt ttgatcgctt ttatatatta cgcgagtacc cgaaatgaca
      961 atgtccgcaa tagacgatga actaaaagcc atacaggtta cgaagcataa agctgttgta
     1021 tgtttggtgt taaaaaacat aaaccatccc tgacagagtt aattaaaaca gggaataata
     1081 taaaaataga aattattaac actattccct gtatattcat tcaatcaatt taattggtga
     1141 atacttactg ataagtaatg gtgtatgcag tgttagtttt tacataacca gcagttgctg
     1201 tttggtctgc gacggcacga acgtaagacg ctttaaaatc ataaacagca gattttgtcg
     1261 ttgcatccag ggctttagta tacacatcgc cagggttgtt aatttgaacc tgtttgatag
     1321 tggaaccatc aatattgtgc agagcgatat tcacgccgtc agacgggttg ttaatagaca
     1381 tgtcgttgtt cagcgttcct ttgctattac caaagaaaac agaaccgaag gtcattttag
     1441 ctgtactacc tggattcgga tttgctttgc tgcagtcaac ggtgatgctg aaaggtttgg
     1501 ctttagcgcc aacggtgtca ttcagcacgc cagcatcgat ttcaccaaca gttgcagttt
     1561 gcagcaggat cagaccatct ttattaccgc cgtcaacgcg ggtttcacag gtgttatcaa
     1621 caaccagacc actgatattt aactgaccac catccatatc agcagaagca gtacctgcaa
     1681 ccattgccag cataagacca gacagggcaa aacctaattt tttagacata aaaatccttg
     1741 aataaaattc cattagtcag actactggtt tgaagaatga ctattcatga cacaaatgtt
     1801 ataagcagat gctagatatt aatgagcaat gatatttgtt accaaaattt acaaccattg
     1861 ttcattagga cgcctattg
//
LOCUS       DAZOXR010000362         1852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZOXR010000362 DAZOXR010000000
VERSION     DAZOXR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.369"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1085)
                     /gene="ipaHd"
                     /locus_tag="ACHUNN_004531"
     CDS             complement(<1..1085)
                     /gene="ipaHd"
                     /locus_tag="ACHUNN_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHd"
                     /protein_id="HFR0578682.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDT
                     VSARNTSGFREQVAAWLEKLSASAELR"
     gene            complement(1686..>1852)
                     /locus_tag="ACHUNN_004532"
     CDS             complement(1686..>1852)
                     /locus_tag="ACHUNN_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0578683.1"
                     /translation="RRISKRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQETLKDT
                     WESADEWFVH"
BASE COUNT          480 a          321 c          467 g          584 t
ORIGIN      
        1 tgtcgaagct ccgcagaggc actgagtttt tccagccatg cagcgacctg ttcacggaat
       61 ccggaggtat tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc ggaaaaggtg
      121 ttggcatgct cttcatgttc aaaagcatgc catatctgtg atacatcaga ttgtttgttt
      181 tccgggaacc atgctgtcac ggcatcagcc agggggcgat ggagtgtatt ctgttgtccg
      241 tcactcatgg agaagtaaat ctgcgggccg tggtagtccg gcgaagaggt taatctttgc
      301 agggattgca gagcatggga tgacaatggg ttatcactaa tatctattga acattcattc
      361 ctcagattaa gaatgctttc cgggatatga cttatctgat ttatgtcaaa ataaccgttc
      421 cttaaatttt ccggcaggta tggaatatcg gttaacatat tgtcgattgc ccatatggag
      481 tgaatgttgt gaggtagagg ggggagttca ctaagaagat tccctattgc gctaatctct
      541 tgtaaagaaa atggtaaagg tggtaaaact gccagcccat tacatgatac atctaatgtt
      601 tccagtagtt ctggtaaaac aggaagagaa cataattgat tatttgagac atgaagctcc
      661 ttcaaagata tggggagtgt gggtagtgtg attagttgat tgtgggacgc attcaataat
      721 ttaagtcctt gaggcaaagc aggcagttca ataagtctgt tatagctgac attaagctgt
      781 gtaagggacg caggcaatgg ggagattaag cttaaattat ttttacttat attaattgat
      841 ttaatttccg gggggatttc aggtaatgtt gtcaggccta attcagacaa gtccaggttc
      901 gtctcttggt tttgtagaca tgatactagt cgctgaaaag cgatgtctcg ttgttcttct
      961 tgtatgcggt tatttttcca ttcagtccaa tgggttagat aattttcata tgcacggcta
     1021 gtgtcgattg aataagtgga gaacgaattt gaaattaatc tgtgattgtt atttgtcggg
     1081 agcataaata tcagggcgta ttctttatgt tgagaggtgt tggattcttt tttatctgct
     1141 ttgcaatgtt attggttccc ttcagcagta gacaggaact tctggaggag gcgaatatat
     1201 agtttgtgta agcatattaa ctccatatgt tatatattga agaacttctg ctttacacta
     1261 tcctaaactg gatcggttta atgtaaaaaa acgatacttt ttgggggagg gggcaatttt
     1321 tccttaaggt atgaaaaaag gagctgaagc tatataatag ctattaatgc cactggtatc
     1381 agtgaatcat gtatataaaa aactgtgtta aggaggctgt ccattagcgg ttttttattc
     1441 ttctggaacc gcggcatctt tgcgtatctt tttgtcttct caccgtctgg tcggtgtttt
     1501 gctgagactg ctaatttcct gtctttgcta gtgctgtact ggtactgccc aatcatgatt
     1561 ggcgggggcg ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt gctgcggctg
     1621 gctggtgaac ttccgatagt gcgagtattg aatgatttcc agccgttact gattttacgt
     1681 gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag agtttcctga
     1741 acaaaagttt tagctgaatc tttatcggcg gtgcgcgtaa cagaaaggcc atcgttgctg
     1801 gtggctttta cgatcacctc tacatcgtca taacgtttac tgatgcgtcg gg
//
LOCUS       DAZOXR010000363         1846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZOXR010000363 DAZOXR010000000
VERSION     DAZOXR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.370"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..282)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUNN_004533"
     CDS             complement(<1..282)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUNN_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0578684.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQA"
     gene            complement(293..>1846)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUNN_004534"
     CDS             complement(293..>1846)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUNN_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0578685.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT          494 a          462 c          383 g          507 t
ORIGIN      
        1 tgcctggttt atttttgacg tataatagat ataggggaat tctcctacat taccagtatc
       61 acaatgacag cgtccaggat atgaggatcc attagttaca gtggtattaa atgtcgttcc
      121 aaccttattt tcgttcgcgg taataagata tcccgagaaa tcaagcgtac ggtgaaatac
      181 gccattatca ggggtacaga tacctttagg agctgcaagt gctacactac ttaacgagag
      241 cataaacaca gcaaagcacc actgtttgat atacttgttc ataaaaatcc ttttaatgac
      301 atgtcacacc ctgttcaatt aatccgctct cactttctgg tgtcgaaagt gaataattga
      361 agatgcattt tgttgaggcg ttttctcccc aggataacgt aacctgtcct ttttctggta
      421 gaccagaaag ataaagttcg ccacggtcac cgacaattcc tgaatctgcc aaacttgcat
      481 cttgtatcgc agcaatggct ccaaaaggta ccggtttacc atttgcattt aacactctga
      541 agagaacgcg ataacccaca tgagtcacaa actccgcttt aaccactgca ccacgcgtgg
      601 gaaccatcgt aacgacggta ttatcaagtt ctatattcga cttcgagaaa tagttatcgc
      661 tgattgctac ccgattttca tcataagggt ttagctgtgt cttgaccgta tagccgcgcc
      721 agtccgtcgc agcaccgcgc atattatcta tttctaaccc ggcggcccca ggtgctttaa
      781 caagagcaat agtttcacct aactcctgac ttaacgttac cccttcgctg tgtgccagaa
      841 ttccccctct gataccataa ttcagcctct gggaattttg gctgtagtta tagctgccgt
      901 tcaccgtgcc ataagagcca tcataggtca gggacatatt accgccaacc tcatcatcat
      961 gactgttata ggactgcatt aaattccatg aaaggttatt tctttccagc gccgtaccgc
     1021 tgagtccgag ttgatgtgag ctaccggaat tatcgttttg cgaaacagaa taggtggcgt
     1081 aactattacc ccaccagccc aataaaggaa cagataaaga gagcatgtag atctgctcac
     1141 tggtgttatc actggcatgg ttgttccccg tcccctgtct ggcagtattg gaataactcc
     1201 aggagacgtt ccacgagaga cgcttccagc tactgctgta gccaaattgc attaatcgtt
     1261 cgacatcatc ggtatgccag tattgttgcc ggcttaatgt caggtataaa ttgccataat
     1321 ctctccccaa cgactgcgtt agcgtcccct ccacgcaact acgtcggtta cctgtttccc
     1381 aaaagtcttc agggctgttg cgccgcgatg cccactcatt gagggtatag tagccctcgg
     1441 tggagtaacg atagccaacc aagcgcaagc tagtgtctgt gtcgtcaaat cgttttgaat
     1501 agagaaagcg atatgactga cccgtttctg tatcatcatg gctaaaattc gcacgagcat
     1561 gtgtcacatc gaaagaaatt gcgcccaaag cgccaagatc tttaccgaca cccagagctg
     1621 cagaaccata attttcagca gcctgcatac ctccatataa agtgatcccg tgggaaaaac
     1681 catgaagtat ttgcgcctga aggacgtccg gtgtaaatcc gtcttcatcg cgcaattcac
     1741 ccacgctgac atcaacatct gtctgacctt cacgtttaag aatcgctaat gaagcataag
     1801 gttgggtgaa tcgttgttcg ctaccgtctt cttctttgat ggttac
//
LOCUS       DAZOXR010000364         1818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZOXR010000364 DAZOXR010000000
VERSION     DAZOXR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.371"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUNN_004535"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUNN_004535"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0578686.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1006..1416
                     /locus_tag="ACHUNN_004536"
     CDS             1006..1416
                     /locus_tag="ACHUNN_004536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578687.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(1450..>1818)
                     /locus_tag="ACHUNN_004537"
     CDS             complement(1450..>1818)
                     /locus_tag="ACHUNN_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0578688.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAZOXR010000365         1781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZOXR010000365 DAZOXR010000000
VERSION     DAZOXR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.373"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            55..597
                     /locus_tag="ACHUNN_004538"
     CDS             55..597
                     /locus_tag="ACHUNN_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0578689.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            677..1351
                     /locus_tag="ACHUNN_004539"
     CDS             677..1351
                     /locus_tag="ACHUNN_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0578690.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1367..>1781
                     /locus_tag="ACHUNN_004540"
     CDS             1367..>1781
                     /locus_tag="ACHUNN_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0578691.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          567 a          321 c          391 g          502 t
ORIGIN      
        1 ccaagagata cagactctta acaaccaaaa aaaaatttta ttaaggaaca tattatgaaa
       61 cgttcaatta ttgctgccgc tgtcttttct tcttttttta tgagcgctgg agtatttgct
      121 gcagacgttg ataccggaac attaactatt aaggggaata ttgcagaatc tccgtgtaaa
      181 ttcgaagcgg gtggtgattc agtaagtatt aatatgccga ctgtaccaac cactgtcttt
      241 gaaggtaaag ctaaatattc tacctatgat gatgcagtcg gtgtaaccag cagcatgtta
      301 aaaattagct gcccgaaaga agttgctggt gtaaaactct cgttgattac caacgataaa
      361 ataaccggta acgataaggc gatagccagt agcaacgata ccgtgggtta ctatctctat
      421 ttaggtgata acagcgatgt cctggatgtt tctgcacctt ttaacattga gagttataaa
      481 acagcggaag gtcaatatgc tattccgttt aaagcaaaat acctgaaact gacagataac
      541 tcagtgcaat caggtgatgt gttatcttct ctggttatgc gtgtggcgca ggattaataa
      601 ataaaattgt aagtttctcc aggtaataaa catggccgct atcccatggc ggccttttaa
      661 tttaagaggc attaaaatga aaggattatt atctttactc attttttcta tggtccttcc
      721 tgcacatgcc ggaattgtta tctacgggac gcgcattatt tacccggcag aaaataaaga
      781 agtgatggtg cagttgatga accagggaaa ccgttcttcg ctgctgcagg cgtggattga
      841 tgatggcgat acgtcattac caccagaaaa aattcaggtt cctttcatgt taacgccacc
      901 agtggcaaaa ataggggcaa attccgggca gcaagtaaaa atcaaaatta tgccgaataa
      961 actgcccact aataaagaaa gcatttttta tctgaatgtt ctggatattc caccaaatag
     1021 tccagagcaa gaaggtaaga atgcactgaa gtttgcgatg caaaacagaa ttaagttgtt
     1081 ttaccgacca gcgggtattg ctccggtaaa taaagcgaca tttaaaaaat tgctggtaaa
     1141 tcgcagtggc aatggtttgg tgataaaaaa tgactcagct aattgggtga cgatttcgga
     1201 tgtcaaagct aataatgtca aagtcaatta tgaaactatt atgattgccc ccttagaaag
     1261 tcagagtgtt aatgtcaaaa gtaataatgc aaataactgg catctgacca ttatcgatga
     1321 ccatggcaac tatattagtg acaaaattta attgcaggag ctgcctatgt tgagaatgac
     1381 cccacttgca tcagcaatcg tagcgttatt gctcggcatt gaagcttatg cagctgaaga
     1441 aacctttgat acccatttta tgataggtgg aatgaaagac cagcaggttg caaatattcg
     1501 tcttgatgat aatcaaccct taccggggca gtatgacatc gatatttatg tcaataagca
     1561 atggcgcggg aaatatgaga ttattgttaa agacaacccg caagaaacat gtttatcaag
     1621 agaagttatc aagcggttag gcattaatag cgataacttc gccagcggta agcaatgttt
     1681 aacatttgag caacttgttc agggtgggag ctatacctgg gatatcgggg tttttcgtct
     1741 cgatttcagt gtcccgcagg cctgggtgga agaactggaa a
//
LOCUS       DAZOXR010000366         1742 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZOXR010000366 DAZOXR010000000
VERSION     DAZOXR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.374"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1352)
                     /locus_tag="ACHUNN_004541"
     CDS             complement(<1..1352)
                     /locus_tag="ACHUNN_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR0578692.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     "
     gene            complement(1441..>1742)
                     /locus_tag="ACHUNN_004542"
     CDS             complement(1441..>1742)
                     /locus_tag="ACHUNN_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578693.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          539 a          342 c          312 g          549 t
ORIGIN      
        1 cttaaatcat gagggtattc agttaattgt gtaattttaa ttgcaacatt attaaatgtg
       61 ccataaaact cacacgggcc ataaaataat gcagtaaaac cattgttatt ttcttgaaac
      121 atattttcga caaaagccgg tatggttctt gctgtattcg gtacgcagca gacagcagcc
      181 tcctcatgag taggggagta tttatatctg gtattaaact gattactgtc aggatgtttc
      241 ctttcatttg cttcataaca attatcagtc ttacagtaca ttatcgcgtg aagtatggga
      301 tgctgcatgc caaaactagc attatagaat agccactcag catcatcggc cagaccaata
      361 ttagtggtca gttttattaa cataacataa gtgctaaata gttcatgaac agaacaatat
      421 tcatagcctg tataagtggc attagctgtg cgtcctgtaa tccactcatc accaatggga
      481 gcgccagatg gtgtaagata atatttcaac tgggataaaa aaatatctaa tgcagtttga
      541 tactgcggcc gttggctcgc tgcgatggct aatgccctaa tatgctcata cgtatgtacg
      601 ccatgacctt taagaaggta accagggtct atgacatttt tatattgcag atcttgctcc
      661 gacacgcatc ctgcggaata atcttcatac agccacaagc aatatgagag atattcaatg
      721 ttattggtta tttcataaag gcggtagaaa acatctgtta ttgttaatcc gtggctatgt
      781 ccagcaaaac cctgttccac aacgaaagga tgtgacgttt ctttagggta gccagccata
      841 atgcattgtg cggcttgggt aagagaagtt aataaactct cattacgggt ggactcataa
      901 catccaagca caaaacgcaa ggcagtagct tgtgcccaga actctccgtt ttctgcttta
      961 cagttaaatt ttaatgatga atcataaatt ccaatgtagc catcatcatc ttgcgtatcg
     1021 ataagttcca aacataaatc gctcgctttt tcctgccatt ctttattatc taacaagtag
     1081 gcactgcggc aatatccatc cttccaattc gattgagttt cactatccca ccaataatat
     1141 tgcatcatgt tatcatcggg attattctgt tttttaataa cgccatcttc agcagataca
     1201 gtgcgtggag ttatacgatc tcttccatat attttatgct ccgtaataag atggggaact
     1261 aatttatcca gttctcctat atgaccgttc atataccatt ccatcttatt ctttaaccaa
     1321 ccagtgggct ttatatcgtt aaaaaaaatc ataaatacac ctttgcataa aaaagtttag
     1381 acgttataaa agacaaatag attgtgatcg cctacagacg atcacaatga aaggaaaata
     1441 ttaacggttt ttgctatcac tcaaatgttt ttcatcctgc accatatcat tgccagattc
     1501 atgcaaagta tttattaatg cattggcatc tggatattgt tgctgatact tggttagata
     1561 tatatcacta tggatatgtt cggcaattat attgataagc aagcgattat tcgagtataa
     1621 ccccagtgat tcagctcgtt gtgaaaaaac cattactgtc ttcatatcaa caacaagcat
     1681 aaaacggtga gaagggtaat attcttcttc atggttaaag cggtaataca gttctacacg
     1741 tg
//
LOCUS       DAZOXR010000367         1731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZOXR010000367 DAZOXR010000000
VERSION     DAZOXR010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.375"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            71..166
                     /locus_tag="ACHUNN_004543"
     CDS             71..166
                     /locus_tag="ACHUNN_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR0578694.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHUNN_004544"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="ACHUNN_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR0578695.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          642 a          287 c          277 g          525 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc g
//
LOCUS       DAZOXR010000368         1726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZOXR010000368 DAZOXR010000000
VERSION     DAZOXR010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.376"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(76..1026)
                     /gene="corA"
                     /locus_tag="ACHUNN_004545"
     CDS             complement(76..1026)
                     /gene="corA"
                     /locus_tag="ACHUNN_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0578696.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1178..1234)
                     /gene="ysgD"
                     /locus_tag="ACHUNN_004546"
     CDS             complement(1178..1234)
                     /gene="ysgD"
                     /locus_tag="ACHUNN_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0578697.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1396..>1726
                     /locus_tag="ACHUNN_004547"
     CDS             1396..>1726
                     /locus_tag="ACHUNN_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578698.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          457 a          439 c          428 g          402 t
ORIGIN      
        1 caatccgcat gactttaaat aagacaattt ttagaaataa gccggataaa aaaatagctt
       61 tatccggcac tcatattaca gccagttctt gcgcttaaag tacagatacg gtgccaggcc
      121 cgcgaggatc ataaagataa tcgcgcctgg gtagccgaaa ctccacttca gttctggcat
      181 aaactcaaag ttcatcccat agctggaagc aacgagcgtc ggcggcagga ataccacgga
      241 taccaccgag aagattttga tgatgcggtt ctgctcgatg ttgataaaac ccatcgccgc
      301 ctgcatcagg aagttcacct tctggaacag ggattcgtta tgcggcagca gggattcgat
      361 atctcgcagg atttcacgcg cctgctccag ttgcccaccc ggtaaacgcg ctttacgcac
      421 caggaagttg agcgcgcgct gggtatccat cagacacagg cgaactttcc agccgatatc
      481 ttccagttcc gccagagtgg agagcgcctc gtcgtactca tcgccctgat gcccttccat
      541 aatcacccgg ctcaactgct ccaggtcgct atagatattt tcaatttcat ctgccaactg
      601 ttcgattttg gtttcgaaca gatccagcag cagctcgtag gcgttaccgt ctaccatcga
      661 ctggctacgg gcacgcatac gatacagacg aaaagcgggc agttcacgct cacgcagagt
      721 aaacagacga ccatcacgga tggtaaatgc cacagtggag ttaccggcgt gatcttccgc
      781 atcttcaaag aagaagaagg agtgaatatg caggccgtcg tcgtcttcaa agaaacgtgc
      841 cgatgcttcg atgtcttcca gttccgggcg ggttgccagg ctctggccaa gttcagattg
      901 tacgcgcagt cgctcgtcgt cgtccggttc gacaagatca atccatactg catttacaag
      961 gggttgtgac tcttcgactt ccagccgggt cagtcggtta ttttccagtt gaaatgcgct
     1021 cagcatgacc gggactccca atgcttaaaa tatcggacag ttcggtgggc acacagaaac
     1081 aaattgggtt tcagaccatt aaacagcctg actcagcgcg acgggaaaaa ttgaggtcgc
     1141 tgacaaccgc taaggctatc agcaaaaagg gatagcctta ggagttgatc ctggatgaca
     1201 ggatagtgag ccagtatcta ctgggtgtgt ccaaggcgaa tgtcctctta gagtgatcgt
     1261 gcgcgcatgt tacgccagca aaattttgcc gtcaacacgc aacagaacac cacagaacaa
     1321 tagttgaact tcgcgctttt tggtactcgt caaggtcgct aacaggataa aaaaatccaa
     1381 catccaggac ctataatggc gcatcggcta ctcattggta aaggaatgat caccttgaat
     1441 ctcaaacgga tttttctcgc cctcacctta cttcccttat ttgctgttgc cgctgatgat
     1501 tgcgcactct cagatccgat gctgaccgta caggcgtata ccgttaatcc tcaaacagag
     1561 cgggtgaaaa tgtactggca aaaagccaat ggcgaagcgt ggggaacttt acatgctctg
     1621 ctggtggata tgaatagtca gggtcaggtg cagatggcga tgaacggcgg catctatgat
     1681 gaaagctatg cgccgctcgg tttgtacatc gaaaacggtc agcaga
//
LOCUS       DAZOXR010000369         1678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZOXR010000369 DAZOXR010000000
VERSION     DAZOXR010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.378"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(19..300)
                     /locus_tag="ACHUNN_004548"
     CDS             complement(19..300)
                     /locus_tag="ACHUNN_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578699.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(428..662)
                     /locus_tag="ACHUNN_004549"
                     /pseudo
     CDS             complement(428..662)
                     /locus_tag="ACHUNN_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          474 a          371 c          314 g          519 t
ORIGIN      
        1 tcgtttgcac tgtctctgtt attcaggcaa tttcaataaa ggcacttgct cacgctctgt
       61 cattttctga aactcttcat gctgcatttc gatttttctt ctaaaatcat aacttgcata
      121 cctgttcttt ttatgtatat ccagatctat ttttgtgacg agttcaaacc acaatgatag
      181 cactccccat gcagcgttat gaaacatttc cgcaacagat ttagagtctg tattaaccgc
      241 ttcagtcatg tagtcgctaa tacgttctgc agtgattttc gttatttcat cgtaattcat
      301 tttatcccct acaagactat ttaatggaat gaaaaatatt tggtctgata acaatgctcc
      361 tgaattaaca cctgcaggct atgttaaagt tgccctcaca cacaatctac ctgttttctc
      421 gtgtgtgtca caggcgcaat acataaatag tctggccatt aagttcgtaa cggatttcgt
      481 actcgccagc aaaaatccgt ctgacatccc tggtttcaaa ctgaaacaac tgttcatccc
      541 atacgtggat gagttaacag aatgaccggc gcctgtgtca gggactacac cgttttggct
      601 gcagctggtt tactgaccaa cgccagaaaa tcatataact gcgtcaatgt cagcgtatcc
      661 agcgcgactg tattattttt ctctgcaacc agaatattgc attaggttat taccaggtgt
      721 tctaatgtac aggtaaagac gttctttata atctgaaatc tctactttta atccctcaga
      781 ctttgcctgc tgaaagccct cgtcaaggtg cgcttttatt aggttaatgt ctggatgtct
      841 tgtatcgggg gcaattctgt attggtgtaa cgatggggtg gatgttccct gcaataagac
      901 ctccagatag agattggcac aaccattccc attatttcta atagtttatt ggtaatcaca
      961 aactgtatat ttagccatta tattgaatcc tgtgaagtaa acggcgacgg ctcaaaattt
     1021 tatcaaacct aagataattc taccatcaaa gttaataatt tgaatttata tgacggctct
     1081 atgggggggt aaaaatcccc gtgcagagag gcgattttca gagttgaaaa cgtcgtcgtt
     1141 aagaacacca aagtcagtac caccaacccg gaaagtagct tcatgcaccg ggacaacagc
     1201 agttttggtg tccgaaatcg tacaattctg tacatctacg caaggaaatg tacggaagta
     1261 agccaaatgg ttatcggacg gtcggaacat ttcgtacagc ctttacgttc agtgagttag
     1321 ctggcctaga tgtaaccact cgttcaactc aattctctta gcgtgggaaa atttccgttt
     1381 tccaagcggc cccctcaagc ggacccctta tagtacaata attttcgata tccaaactgc
     1441 ccccaattac atctccagtc tctggcagca gagatcatat atttccattg ccgccgtagc
     1501 cacgatattc tggctattca caaaaacaat ttagccccaa agcgcagtaa aaaagtacac
     1561 tatgaaggtg tatgatacct tcatagtgct taagtaatta tgagtaatat aatctgtgta
     1621 aactactaat tgcttcaccg tcagtgatta aatgcgtagc tgttccttgt cttatata
//
LOCUS       DAZOXR010000370         1676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZOXR010000370 DAZOXR010000000
VERSION     DAZOXR010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.379"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..889)
                     /locus_tag="ACHUNN_004550"
     CDS             complement(<1..889)
                     /locus_tag="ACHUNN_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0578700.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="ACHUNN_004551"
     CDS             complement(989..1345)
                     /locus_tag="ACHUNN_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0578701.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="ACHUNN_004552"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUNN_004552"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAZOXR010000371         1668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZOXR010000371 DAZOXR010000000
VERSION     DAZOXR010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.380"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            66..1319
                     /locus_tag="ACHUNN_004553"
     CDS             66..1319
                     /locus_tag="ACHUNN_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0578702.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1368..1487)
                     /locus_tag="ACHUNN_004554"
     CDS             complement(1368..1487)
                     /locus_tag="ACHUNN_004554"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0578703.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          345 a          424 c          407 g          492 t
ORIGIN      
        1 aacaattcga ttttcctaac ctatgtataa gcctgatcca caggcatatt tagcaaggat
       61 ttcaagtgag ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa
      121 gcatttctca gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc
      181 tggcgctgtt ctctgccgga ctggcaacct ttgcactcct ctattgtgtg cagcctatcc
      241 ttccggtgct ttcgcaggag tttggcttaa cccccgcgaa cagtagtatt tcactgtcca
      301 tttccacggc gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg
      361 gtcgcaaacc agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga
      421 caatgatgac cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa
      481 gtggcgtggc agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg
      541 ccttttcgat ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa
      601 ttagtggtgt cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt
      661 tcgcgctagc ctcggcgttg atgttctgga aaatccttcc ggaatcacgc cattttcgcc
      721 cgacttcgct gcgccctaag acgttattta tcaactttcg tctgcactgg cgtgaccggg
      781 gattaccgtt attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta
      841 attacatcgg ctatcggttg atgctttccc cctggcatgt cagtcaggcc gtggttggct
      901 tattatcgct ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca
      961 cccgctacgg gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttac
     1021 tgatgacctt attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag
     1081 gattcttcgc agcccactca gttgccagca gctggattgg cccccgcgca aaacgcgcta
     1141 aaggccaggc ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga
     1201 cgctgggtgg tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc
     1261 tgatgctggt cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa
     1321 aaataagccc ggactgcggt aactacacgt ccggacttat tgcttgctca aaccaacgtt
     1381 aatagccatc ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc
     1441 aggattataa gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc
     1501 gcagttattc taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat
     1561 tggtagctaa atttccctta agtcacaata cgttattatc aatgctgtaa tttattcagc
     1621 gtttgtacat atcgttacac gctgaaacca accactcacg gaagtctg
//
LOCUS       DAZOXR010000372         1667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZOXR010000372 DAZOXR010000000
VERSION     DAZOXR010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.381"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUNN_004555"
     CDS             complement(<1..105)
                     /locus_tag="ACHUNN_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578704.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGK"
     gene            complement(183..604)
                     /locus_tag="ACHUNN_004556"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="ACHUNN_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            complement(588..815)
                     /locus_tag="ACHUNN_004557"
     CDS             complement(588..815)
                     /locus_tag="ACHUNN_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0578705.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="ACHUNN_004558"
     CDS             892..1299
                     /locus_tag="ACHUNN_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0578706.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="ACHUNN_004559"
     CDS             1503..1658
                     /locus_tag="ACHUNN_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0578707.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          470 a          436 c          344 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAZOXR010000373         1663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZOXR010000373 DAZOXR010000000
VERSION     DAZOXR010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.382"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            104..358
                     /gene="bssS"
                     /locus_tag="ACHUNN_004560"
     CDS             104..358
                     /gene="bssS"
                     /locus_tag="ACHUNN_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0578708.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            473..1591
                     /gene="solA"
                     /locus_tag="ACHUNN_004561"
     CDS             473..1591
                     /gene="solA"
                     /locus_tag="ACHUNN_004561"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0578709.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT          429 a          411 c          413 g          410 t
ORIGIN      
        1 attcggtgaa tacccttact tattggtata ctgaagttgc ttcagataaa aacgcattga
       61 acctcgaata acgttgtcta gtaacacgaa ttagggggcc atgatggaaa aaaataatga
      121 agtcattcag actcatccgc tcgtagggtg ggacatcagc accgttgata gctatgatgc
      181 gctgatgttg cgtttgcact accagacccc aaataagtcc gagcaggaag ggactgaagt
      241 tggtcaaacg ctctggttaa ccactgatgt tgccagacaa tttatttcga tattagaagc
      301 aggaatcgcc aaaattgaat ccggtgattt ccaggtaaac gagtatcggc gtcattaaca
      361 tgacatgacc atccacccaa aaggcacctc accggtgcct ttaccatttc tgggttgtat
      421 atctgcattc agttaattac tctgcttaaa ccatgagagc agagagaaaa aaatgaaata
      481 cgatctcatc attattggca gcggttccgt aggcgctgcc gccgggtatt atgcaacccg
      541 cgccggttta aacgtgctaa tgaccgacgc ccatatgcca ccgcatcaac acggcagcca
      601 ccacggcgat acgcgattaa ttcgccatgc ttatggtgaa ggcgaaaagt atgtcccgct
      661 ggtcctccgc gcgcaaacgc tgtgggatga actttcccgc cacaacgaag atgatcccat
      721 ttttgtacgc tctggtgtca ttaacctcgg cccggctgac tccgcatttc tcgccaacgt
      781 cgcccacagc gccgaacagt ggcaactcaa cgttgaaaag ctcgatgcgc aagggattat
      841 ggcccgctgg ccagaaatac gcgtcccgga caactacatc ggcttatttg agactgattc
      901 cggttttttg cgcagcgaac tggcgattaa aacctggatc caactggcga aggaagcggg
      961 ctgtgcgcaa ctgttcaact gcccggtcac cgcaattcgt catgacgatg atggcgtaac
     1021 tattgaaacg gttgacggtg agtatcaggc gaaaaaagcg attgtctgcg cgggaacatg
     1081 ggtaaaagac ctgctcccgg agctgcctgt ccagcccgta cgtaaagtat ttgcctggta
     1141 tcaggccgat ggccgctata gcgtgaagaa taaattcccg gcgtttaccg gtgaactgcc
     1201 caatggcgat caatattatg gttttccggc agaaaacgac gcgttgaaga ttggcaaaca
     1261 taacggaggc caggttatcc attcagcgga tgaacgtgtt ccgtttgcgg aagtggtcag
     1321 cgatggttcg gaagccttcc cgttcttgcg caatgtattg ccgggtatcg gttgctgcct
     1381 gtacggcgct gcctgcacct atgataattc gcctgacgaa gattttatta tcgataccct
     1441 acccggccac gataatacac tgctcattac cggcctgagt gggcacggtt ttaaatttgc
     1501 gtcagtttta ggggaaatag ctgccgattt tgcgcaagac aaaaaaagcg attttgattt
     1561 gacgccattc aggctttccc gcttccaata atcatcatta cggcctccag aatgaggccg
     1621 tttttacagg agtatttaat gcgtcggctg ttgcactatc tca
//
LOCUS       DAZOXR010000374         1650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZOXR010000374 DAZOXR010000000
VERSION     DAZOXR010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.383"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(132..785)
                     /gene="ribB"
                     /locus_tag="ACHUNN_004562"
     CDS             complement(132..785)
                     /gene="ribB"
                     /locus_tag="ACHUNN_004562"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0578710.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(889..1037)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1159..1449
                     /gene="ubiK"
                     /locus_tag="ACHUNN_004563"
     CDS             1159..1449
                     /gene="ubiK"
                     /locus_tag="ACHUNN_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0578711.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          478 a          410 c          358 g          404 t
ORIGIN      
        1 tgcctgatat taatgaaatt ttctcataga aagagaagcg gaattaacat cttgcatagc
       61 agggcaaaat aaaaaccgaa gccagctaca acttcggttt cttgattaag gcagtaatta
      121 agcagcggtt ttcagctggc tttacgctca tgtgcctgac ggtatgccac caggtcttca
      181 atagtcacga gcgccatatt gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc
      241 atcgtgccat cgtcattagt cagctcacac agcacaccag ccggtttaaa gcctgccagc
      301 gtcatcaaat caatagtcgc ttcagtatga ccgccacgcg tcagtacacc acctgcctgc
      361 gcgcgaagtg ggaaaacgtg gcccgggcga ttcagatcag aaggttttgc gccatcggca
      421 atcgctgcgc gaacggtcgt aatacgatca gcggcagaaa caccggtagt cacaccttca
      481 gccgcttcaa tggtcacggt aaaaccggtg ccataggcgc tggtgttatt ttctaccatc
      541 attggcagat caagttgttt acggcgatct tcagtgatgc acaggcaaac aataccgcta
      601 ccgtggcgaa tggtcagcgc catctgctca acggtcatgg tttctgccgg gaagatcata
      661 tcgccttcgt tttcacggtc ttcatcatca agcaccatta caccgcgtcc ttcacgcagc
      721 gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga
      781 ttcatggtaa aaaaacctca ctaaaattat ggttaccaga atcagggcag tcttaggagt
      841 ggcggcatat agccaaaata acgtgagcgg gtccatgccc gacagaatcg ttactctctc
      901 ccatccggac tctaaccgtc ggccccggaa ttacaccgga tctgctgtcc tttgagttcg
      961 cacccaaagc gctcgcgggc tttcaactga gttgatttac cgccggtggg gaatttcgcc
     1021 ccgccctgag aataagcgga ttcactataa cgctaatgat tagcggcagc aacgcatagc
     1081 ttcacataat tctggtttat gacttaccct tatcgcacta caatggtact caacacctat
     1141 cagtacaggg aaactacaat gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt
     1201 cacgaatcaa tgcctaaagg aatcagggag ttcggggaag atgtggagaa aaaaatccgc
     1261 caaaccctac aagctcagct gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc
     1321 caaacgcaag tcctgttacg gactcgtgag aaactggcgc ggcttgaaca aagaatgagc
     1381 gaactggaaa atcgcagtac tgagattaaa aagcagccag atccagagac tctcccccca
     1441 acgctgtaaa catttgtctt taatgcactg taaattatga attaatcttc taatttacag
     1501 tgctttctat taccttatcg cttaattcca ttcagaatat catcacaaaa atgaatgagt
     1561 gcaattgaaa atataattgt tatgtatttt ttaagtttta cttaatccat atttagataa
     1621 actaatttca taaaacccga aaagtaaaat
//
LOCUS       DAZOXR010000375         1649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZOXR010000375 DAZOXR010000000
VERSION     DAZOXR010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.384"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHUNN_004564"
     CDS             <1..275
                     /locus_tag="ACHUNN_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0578712.1"
                     /translation="WSKIKLIIWYQSDAFLPPESIFTLTHTGIMLNNKVLPVTIYNVV
                     PFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQSP"
     gene            complement(556..687)
                     /locus_tag="ACHUNN_004565"
     CDS             complement(556..687)
                     /locus_tag="ACHUNN_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0578713.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1187..1263)
                     /locus_tag="ACHUNN_004566"
     tRNA            complement(1187..1263)
                     /locus_tag="ACHUNN_004566"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1227..1229),aa:Arg,seq:tct)
     gene            complement(1364..1439)
                     /locus_tag="ACHUNN_004567"
     tRNA            complement(1364..1439)
                     /locus_tag="ACHUNN_004567"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1404..1406),aa:Ile,seq:cat)
     gene            complement(1542..>1649)
                     /locus_tag="ACHUNN_004568"
     CDS             complement(1542..>1649)
                     /locus_tag="ACHUNN_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0578714.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          514 a          425 c          302 g          408 t
ORIGIN      
        1 tatggagtaa aataaaatta ataatctggt atcaatcgga tgctttctta cctcctgaaa
       61 gtatatttac actgactcac acaggcatca tgctcaataa caaagtgcta cctgtaacca
      121 tttacaacgt agtaccattc aataaaacat tctggaattc aatcaaaaat agccaggaat
      181 gccctacaaa tacagataac gtattgaatg aatgctttaa taaccgttgc actctgcaaa
      241 tatgtcctta tggactaaaa caacaaagtc cataaggagt ttactcacat ctgacaaaat
      301 caatataaac agcccctccg gagaggggct ggagagtggc gctatgtgcc attgcatggt
      361 gccgggtgcc tcccggtgaa ttcagtacca gcacctgaat ccgcgattat cccatatacc
      421 tactcgctga ttgcccctcc gcacaggggg attcaccatg ccagtttctt ttaacaaact
      481 ccccgcaaac cagacaacag tcaaccgcct gaattgtgaa gtatttaaaa atttctcccg
      541 ctaactgata cccggctaac agtctggcgt tttctttttc agcaacggga aagcaacaac
      601 caccacaccc gccaccagca caccgtcagc cagcactgac attatccggc tgctgcaatg
      661 ccattcacaa aaacagtaag caatcacttt ttaccgtaac aggtgataat ccagatatgt
      721 atctacccca gatgagtaat ccgaagttca tccataccac aggtcctggc tattctgttg
      781 tactcctgaa caagagcaaa taattctgaa ttagcaacca tgaactcatc gcaaaccctc
      841 tgtatagcat cactattcag aataataacg tctcttcccg aaagacgatc aggagtacag
      901 aacaaaactg tcaaacggct gaaggccttt gctcgtgctg cattgactat atcaatacgc
      961 tgcctaagga tgaaacaccc cgacgcctca tcaatattca ctctacccac accatatgaa
     1021 tgataaatat ttaatgctga aaaaaccatt agaccgtata acaaacactc aatcaatact
     1081 taacagaact tttatttttg acaaacataa aatattttca acaatatcct gagccaggta
     1141 tatttcagta taaggctctg ccggaaggaa tctggaagaa tgaatatggc gcgctgtact
     1201 ggattcgaac cagtgaccga ttgcttagaa ggcaattgct ctgtccggct gagctaacaa
     1261 cgctgaatac cgataatgga ccgccatcgg ggacccgccc ccgcaccaac aaccctgtta
     1321 tcgtgtcgtc tgctcttcct gataagctaa tggcggtttg tgatggtggc ccttgctgga
     1381 tttgaaccag cgacttggcg attatgagtc gctcgctctc accactgagc taaagggccg
     1441 ggagcagaat aataacggtc cgtaattaat tccgcaataa aaaacccgct cggcggcggg
     1501 ttgtagaaac tcttctaacg tcaggcataa aacgcccata gttatgacga atttaccaca
     1561 gattccggaa aaatcaacct agttaccttt tttaactgcc gctcagccca tgcttcttca
     1621 atatcaaacc gagtcaccag cgcatcata
//
LOCUS       DAZOXR010000376         1640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZOXR010000376 DAZOXR010000000
VERSION     DAZOXR010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.385"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            182..625
                     /gene="cmtB"
                     /locus_tag="ACHUNN_004569"
     CDS             182..625
                     /gene="cmtB"
                     /locus_tag="ACHUNN_004569"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR0578715.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            653..>1640
                     /gene="cmtA"
                     /locus_tag="ACHUNN_004570"
     CDS             653..>1640
                     /gene="cmtA"
                     /locus_tag="ACHUNN_004570"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR0578716.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          390 a          359 c          402 g          489 t
ORIGIN      
        1 tatgcatcac gatatgcgta ttgacatttg ttgttatatc tataactcaa tgttatataa
       61 gaaattaata attcactgtt ttcaaaacac cggtttccct gctcaattgc tttcattaaa
      121 ccgcacgagt attttatttt taccgccgga cggtacccta aaaataaaat aaggataatt
      181 tatgcggctt agtgactatt ttccagaatc atcaatctca gttatacatt cagcaaaaga
      241 ttggcaggaa gctatcgatt tctcgatggt atcattgctg gataaaaact atatcagcga
      301 gaattacatt caggcaatta aagattccac cattaacaat ggcccttatt atattctcgc
      361 accaggcgtg gcaatgcctc atgcgcgacc ggaatgtggg gcgcttaaaa ccgggatgtc
      421 tttgacatta cttgaacaag gtgtttattt tccggggaat gacgaaccaa taaaattact
      481 catcggcctt tctgctgccg atgccgattc gcacattggc gctattcagg cgttaagtga
      541 attactgtgc gaagaagaaa tactcgaaca actcttaaca gcagcatcag aaaaacaatt
      601 agcggacata atcagccgcg gataattact ctctcctcta caggataata ttatggaaaa
      661 caagtctgct cgtgcaaagg tccaggcttt tgggggcttt ttgacggcaa tggtcatccc
      721 caatattggt gcttttattg cctggggttt tattactgcg ttatttattc ccaccggttg
      781 gctgcctaac gaacatttcg ccaaaattgt cggcccgatg attacctatt tattgcccgt
      841 gatgattggt tctaccggtg gtcatctggt cggcggtaaa cgcggcgcgg tcatgggcgg
      901 aataggtact attggtgtga tcgtcggcgc agagatcccg atgttccttg gctcaatgat
      961 tatggggccg ctcggtgggc tggtcataaa atatgttgat aaggcactgg aaaaacgcat
     1021 acctgccggt tttgagatgg ttatcaataa cttctcatta ggtatcgcgg ggatgctcct
     1081 ttgtctgctg ggttttgaag ttatcggccc ggcggtgtta attgccaata ctctcgtcaa
     1141 agagtgtatt gaggcgctgg tacatgcggg ttatctgcct ctgctgtcag tcatcaatga
     1201 accggcgaaa gtgcttttcc tcaataatgc gatcgatcag ggcgtctatt atccgctggg
     1261 aatgcaacag gcttcggtta acggtaaatc catcttcttt atggtggcgt ccaatcctgg
     1321 gcctggctta gggctgctgc tggcgtttac cttgtttggt aaagggatga gtaaacgttc
     1381 tgcgcccggg gcgatgatta ttcacttcct cggtgggatc cacgaactgt atttcccgta
     1441 tgtgctgatg aagccgctga ccattattgc catgattgcg ggcggtatgt ctggcacctg
     1501 ggtgtttaac ttactggacg gtggtctggt ggctggccca agtccggggt ctatctttgc
     1561 ttacctggca ctgacgccga aaggctcgtt cctggcgaca attgccggtg ttacggtagc
     1621 taccctggtg tcctttgcta
//
LOCUS       DAZOXR010000377         1618 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZOXR010000377 DAZOXR010000000
VERSION     DAZOXR010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.386"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHUNN_004571"
     CDS             complement(<1..202)
                     /locus_tag="ACHUNN_004571"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0578717.1"
                     /translation="MAEPMAQRPICRQNSHRLHDRLGEPQYTDSLHSQGSASSMWMRH
                     VGGHERFRTGDGQLNTQANRYVL"
     gene            complement(162..1618)
                     /locus_tag="ACHUNN_004572"
                     /pseudo
     CDS             complement(162..1618)
                     /locus_tag="ACHUNN_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT          364 a          464 c          362 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttac
//
LOCUS       DAZOXR010000378         1590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZOXR010000378 DAZOXR010000000
VERSION     DAZOXR010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.387"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..521
                     /locus_tag="ACHUNN_004573"
     CDS             <1..521
                     /locus_tag="ACHUNN_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0578718.1"
                     /translation="EEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDELIDF
                     TFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENK
                     LHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRN
                     YLASVLAGSGLS"
     gene            532..969
                     /locus_tag="ACHUNN_004574"
                     /pseudo
     CDS             532..969
                     /locus_tag="ACHUNN_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            969..1346
                     /locus_tag="ACHUNN_004575"
     CDS             969..1346
                     /locus_tag="ACHUNN_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578719.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          527 a          241 c          402 g          420 t
ORIGIN      
        1 ttgaggaaaa atttccgctg aaagaattaa ataatccaga gcatgacagt tacgcaatga
       61 gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg
      121 aacttattga tttcacgttt gaatggaaag gactgaagaa attatgcgtg gcagtctcct
      181 ttcggtcaat aatagcagaa caaaagaaag agccagaaat gacggtcaga tattatatca
      241 gttctgctga tttaaccgca gaaaagttcg ccacagcaat ccgaaaccac tggcacgtgg
      301 agaataagct gcactggcgt ctggacgtgg taatgaatga agacgactgc aaaataagaa
      361 gaggaaacgc cgcagaatta ttttcaggga tacggcacat cgctattaat attttaacga
      421 atgataaggt attcaaggca gggttaagac gtaagatgcg aaaagcagcc atggatagaa
      481 actatctcgc gtcagtcctt gcggggagcg ggctttcgta atctttccct agggagtcag
      541 ccgaatgttg ggaaggattt gacggatgaa gagaagaact gcttggccgg cggcagtacg
      601 ggaagcccgg gaggatgggg accagaagat gagaatagcg caagggatcg tatgcagcat
      661 gaacggtata aagatgaatt gcgacagaaa atgggaaaac ccgacgttac agattcaaat
      721 ttgaaaaata ttatagacga tctttatcgg ccaaatgcaa aagtaggaag tggaagcaca
      781 gcagatgctg ttcgttatga attggccaca ggtgaaaaag ttggtggtag aggacatgta
      841 ttgaaagcac aggattatac aagggcttta caggattggt tggataagaa tccaacagca
      901 tctccagggg atcgagcagc agcagaaaac gttcttcagg atttacagaa tgctttggga
      961 ggcaagtaat gaaccaatat attgctagtt atatagaaaa aatggcatca ttttccactg
     1021 ttttgtctga gacagtctca ttagctaatg aatactggca tccagaaacg ccaccggtgg
     1081 ttatgttgtt ctctcaggtt ggaaaaacga ttgtaaaaaa tttttatgaa atgggggata
     1141 ttgaaagaga aaaaatattt aaacatgtcg aagatggtat ggtctcgctt gatgatgaat
     1201 tggccacagt tgtagctaca gggcttatag aggcaatagt cactgcgacc gatagcaatt
     1261 ctttactttg gcaagattta agtaaatttc tgggtgacaa atcaaagtct catgcattag
     1321 tgtggaaaaa ttttggtcag gagtgatatt attcaagaag tattaagctc gctattgaga
     1381 tcttaatgct tcttgagatt aaattaaagt taggacgaag taattgcagg tggaaataaa
     1441 tccatagtga cgtgtggcaa tggctattag atgttatggt gtgatgggct ttggtgattt
     1501 aagagctaag ttagattgcc ttatgaaaaa gtatggttat atatttaatg atgtgtatca
     1561 tatatttaaa taaaaaacgg ttctctcttg
//
LOCUS       DAZOXR010000379         1579 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZOXR010000379 DAZOXR010000000
VERSION     DAZOXR010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.388"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..548
                     /locus_tag="ACHUNN_004576"
     CDS             <1..548
                     /locus_tag="ACHUNN_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0578720.1"
                     /translation="AFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPG
                     DEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTG
                     QTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMES
                     PGKDATPGNVYAQADYKIAYE"
     gene            608..>1579
                     /locus_tag="ACHUNN_004577"
     CDS             608..>1579
                     /locus_tag="ACHUNN_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0578721.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT          489 a          335 c          315 g          440 t
ORIGIN      
        1 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
       61 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
      121 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
      181 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
      241 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
      301 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
      361 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
      421 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
      481 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
      541 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
      601 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
      661 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
      721 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
      781 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
      841 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
      901 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
      961 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
     1021 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
     1081 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
     1141 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
     1201 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
     1261 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
     1321 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
     1381 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
     1441 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
     1501 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
     1561 tttattcaat acattacag
//
LOCUS       DAZOXR010000380         1570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZOXR010000380 DAZOXR010000000
VERSION     DAZOXR010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.389"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1013)
                     /locus_tag="ACHUNN_004578"
     CDS             complement(<1..1013)
                     /locus_tag="ACHUNN_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0578722.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKR"
     gene            complement(1039..>1570)
                     /gene="rfaY"
                     /locus_tag="ACHUNN_004579"
     CDS             complement(1039..>1570)
                     /gene="rfaY"
                     /locus_tag="ACHUNN_004579"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR0578723.1"
                     /translation="CRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLT
                     FPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNM
                     LSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVI
                     YRTKLRKFIKKLKGKA"
BASE COUNT          491 a          326 c          266 g          487 t
ORIGIN      
        1 tttcttttat acttatttaa tttaaattta ataaagtaaa taaaagcgtt aaacgtatca
       61 ccatttttct ttgcatgttg atatttcact tgatactgct tttcattcgt ggctggaata
      121 aacgccacat cattccagca agaagcctga taagcctcat taaaatactt ctggcaggga
      181 taattcgtcc agtcgttcca gggcttcgtt acgccaacat aatgaatcag cttagtatca
      241 tcggtaatgg tctttttata atcttcatcg cttttatttt ttaactcata atcgatgcca
      301 taaaagcaat cataatcgcc gctaataaaa atacaatgac caaagaacag aatatttaaa
      361 atatcctgat caaggcattt atacttcgtg tgctgcgggt cacttgccag catagcaatg
      421 gctttattca taaaatcatt tttcgcccat gcttcaagct gcagatatac tacaccggcg
      481 ttgaaataat tccctggaag tccctctata gccagacgct tcgctggttt ttcctgcata
      541 aaaggcacat ctttaataac agctgcaaac ttatccccat caaaattaat atcaagcaat
      601 ccagctaatg agcctttaca gatgacatca gcatcgatat ataacagatt ttcaatagtt
      661 tcactcagat actggaacgc aactaaacgg aagaatgttg cataagacca ctgtttcagg
      721 ctggggaaga tatcaataaa ggcactggag actttataaa ttcttacttc aacgttagca
      781 tttttgctgg taaaagcatt aacacgttgt agataatctt catcaatgta gtcggtaaag
      841 agatgaaact taaccgcaag gtccggatta tgcataacaa ctgattgcat agatactgcc
      901 gcaccaaata aaaagttacg gtcaataccg taagcgatat ttaacacacg atcggtgtta
      961 gcaatttccc cagagacagc gacttcagta atactctcag ctaataaatc catgttggtt
     1021 ccgttttgac tgtgtggttt acgctttgcc ttttaatttt ttaataaatt tgcgtagttt
     1081 ggttctgtag attactgaat agtagccata atcttttatt tcgttggcaa tacccaggtg
     1141 acgttccatg gccagacgat cacgagcttt acgctctgcc gtacagcttt taccggatag
     1201 gtcaattatc ctgacaccat ctttactaac gataaagtta ccccggtgtg ggtcgcccga
     1261 tagcatattt aaggcatgca atttttccat tgaagcctta atttctgctt taacatcttc
     1321 tggaatgatc ggcatatcat tgagctctac gccctcgaca tactcgataa gcatgataaa
     1381 aacactggca tagttgaaaa ttttacgctc agccagaaaa taaaaatcat taggaaaagt
     1441 aagacctgca cttctgacac gatcggtttc aacgatcaaa ttctgatagt agtcaccttt
     1501 tacaaaagat tttaaaaaac gttcattcct tttcgttttc ggggcaaaga ctttaaaaac
     1561 atagcgacaa
//
LOCUS       DAZOXR010000381         1549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZOXR010000381 DAZOXR010000000
VERSION     DAZOXR010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.390"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            351..476
                     /locus_tag="ACHUNN_004580"
     CDS             351..476
                     /locus_tag="ACHUNN_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR0578724.1"
                     /translation="MWLPVLFRFLVDSLAMLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHUNN_004581"
     CDS             complement(921..1397)
                     /locus_tag="ACHUNN_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0578725.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          359 a          405 c          384 g          401 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacacccca catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctatgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZOXR010000382         1539 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZOXR010000382 DAZOXR010000000
VERSION     DAZOXR010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.391"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1104
                     /locus_tag="ACHUNN_004582"
     CDS             <1..1104
                     /locus_tag="ACHUNN_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0578726.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          385 a          340 c          398 g          416 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt cttcactttt aattatcgta ttcattataa
     1501 aactaagtta aaaaaactcc tagagagtga gttgttaat
//
LOCUS       DAZOXR010000383         1536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZOXR010000383 DAZOXR010000000
VERSION     DAZOXR010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.392"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /locus_tag="ACHUNN_004583"
     CDS             complement(<1..1012)
                     /locus_tag="ACHUNN_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR0578727.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGK"
     gene            1267..1513
                     /locus_tag="ACHUNN_004584"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="ACHUNN_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAZOXR010000384         1516 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZOXR010000384 DAZOXR010000000
VERSION     DAZOXR010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.393"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>1516)
                     /locus_tag="ACHUNN_004585"
     CDS             complement(<1..>1516)
                     /locus_tag="ACHUNN_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578728.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAZOXR010000385         1505 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZOXR010000385 DAZOXR010000000
VERSION     DAZOXR010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.394"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..210
                     /locus_tag="ACHUNN_004586"
     CDS             <1..210
                     /locus_tag="ACHUNN_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578729.1"
                     /translation="GHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPERLVRQ
                     TLFRANRLLMEAYTEILELEACFKD"
     gene            224..658
                     /locus_tag="ACHUNN_004587"
     CDS             224..658
                     /locus_tag="ACHUNN_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0578730.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            709..1485
                     /locus_tag="ACHUNN_004588"
     CDS             709..1485
                     /locus_tag="ACHUNN_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578731.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
BASE COUNT          325 a          411 c          479 g          290 t
ORIGIN      
        1 ggacacgaaa aactgcgcgc cgaagtggcg gaggtggcca atgccatgtg cgacctgcgt
       61 acaaccatga atgagatgga gcagcggtac agctttaatg ccgacaccct gccggaacgt
      121 ctggtgcgtc agacgctgtt tcgcgcaaac cgcctcctga tggaggcata taccgaaatt
      181 ctggaactgg aagcgtgctt taaagattga gaaggagacg agaatgtacg gaacatgcga
      241 aacgctatgc cgggagctgg cagtaaagta tccgggagac atgccgctga tgctggttat
      301 ctggtccccg gaagagattc aggcactggc tgacggtatg gaaatttctc tgaccggtca
      361 tgaaatcagg accgtcctgg cacgcctgga ggacatcccg gaagaccagc ggattgagtc
      421 cggtatttct tccgctgcgg tgatggagat tatccgcaac gagagcgaaa accgcctggt
      481 gaccgtccct gctgaactgc tggcgtccct gattcagacc gctgaacagg cattgtggaa
      541 acgtgaatgg gccgcccggg atcatggcct cgccgtcccg gaatgcgtca cccgccgtca
      601 ggaggtggtt aatcaggccc gcaccctgct gaaaaacaac acacacgaaa acgactgatg
      661 ttatcgccgc ctgccctgcg ggcggcgata cagggagaac gcttaatcat gaacgaaaac
      721 agcacgctga acgcactgat ttgtcgtcac gcccgcaacc tgctgctggc gcagggctgg
      781 ccggaagaga cggatgttga ccagcgtaac ccgaagtatc cgggctggat cagcatttat
      841 gttctgctgg atgcgccccg gctggcgacg ttacttatca accgtcacgg cggcgtactg
      901 ccgccgctcc tggcctccgc cattcagaaa ctgaccggaa ccggggcgga actggtactg
      961 tccggcagtc agtggcagtc gctgccggta cttccggcag acggaacgca ggtgtctttc
     1021 ccgtatgccg gagaatggct gaccgaagac gaaatcaggg ctgtacttgc ggcggtgcgc
     1081 gatgcggtac gcagtgtcag ttgccgggtg gcagaagatg cgcggcgtat ccgggcggcg
     1141 ctgaccacca ccggtcagac gttgctgact cgccagacgc gccgctttcg tctggttgtg
     1201 aaggaaagcg atcacccctg ctggctcgat gaagatgacg aaaacctgcc cgtggtgctc
     1261 gatgccatcg tgaaccgggg agcacgtttt tcgtcggtgg aaatgtacct ggtcagcgat
     1321 tgtattgagc atatcctgtc cagtgggctg gcctgcgatg tgctgcgtat accggatgaa
     1381 ccgccccgcc gctggtttga ccgtggtgtt ctgcgggagg tggtccggga agcccgggcc
     1441 gaaatccgca gcatggcgga tgccctggca aaaatccgga aatgatgacg ttggcggggt
     1501 aaatc
//
LOCUS       DAZOXR010000386         1494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZOXR010000386 DAZOXR010000000
VERSION     DAZOXR010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.395"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..210
                     /locus_tag="ACHUNN_004589"
     CDS             <1..210
                     /locus_tag="ACHUNN_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578732.1"
                     /translation="GHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPERLVRQ
                     TLFRANRLLMEAYTEILELDACFKD"
     gene            191..658
                     /locus_tag="ACHUNN_004590"
     CDS             191..658
                     /locus_tag="ACHUNN_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0578733.1"
                     /translation="MRALKIEKETRMYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQ
                     ALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVIVPAE
                     LLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNTREND"
     gene            703..1473
                     /locus_tag="ACHUNN_004591"
     CDS             703..1473
                     /locus_tag="ACHUNN_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578734.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSECIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
BASE COUNT          327 a          410 c          471 g          286 t
ORIGIN      
        1 ggacacgaaa aactgcgcgc cgaagtggcg gaggtggcca atgccatgtg cgacctgcgt
       61 acaaccatga atgagatgga gcagcgatac agctttaatg ccgacaccct gccggaacgt
      121 ctggtgcgtc agacgctgtt tcgcgcaaac cgcctcctga tggaggcata taccgaaatt
      181 ctggaactgg atgcgtgctt taaagattga gaaggagacg agaatgtacg gaacatgcga
      241 aacgctatgc cgggagctgg cagtaaagta tccgggagac atgccgctga tgctggttat
      301 ctggtccccg gaagagattc aggccctcgc tgacggaatg gatatttccc tgtccgatca
      361 tgaaatcaga accgtcctgg cgcgcctgga ggacatcccg gaagaccagc ggactgaatc
      421 cggtatttct tccggcgtgg cgatggagat catcaataac gtgagcgaaa accgccaggt
      481 gatcgtccct gctgaactgc tggcgtccct gattcagacc gctgaacagg cattgtggaa
      541 acgtgaatgg gccgcccggg atcatggcct cgccgtcccg gaatgcgtca cccgccgtca
      601 ggcggtgatt aatcaggccc gcaccctgct gaaaaacaac acacgcgaaa acgactgatg
      661 ttatcgccgc ctgcgggcgg cgattcaggg agaccatgaa tcatgaacga aacgctgaat
      721 gcactgattt gtcgccacgc ccgtaacctg ctgctggcgc agggctggcc ggaagagacg
      781 gatgttgacc agcgaaatcc gaaccatccg ggctggatca gcatttacgt gcggctggat
      841 gcgccccggc tggcgacgtt acttatcaac cgtcacggcg gcgtactgcc gccgcacctg
      901 gcctctgcca ttcagaaact gaccggaacc ggggcggaac ttgttctgtc cggcagtcag
      961 tggcagtcgc tgccggtact tccggcagac ggaacgcagg tgtctttccc gtatgccggt
     1021 gagtggctgg cagaagacga aatcagggct gtacttgcgg cggtgcgcga tgcaatacga
     1081 agcatctgtt accaggtggc agacgatgcg cggcgtatcc gggcggcgct gaccaccacc
     1141 ggtcagacgt tgctgacccg ccagacgcgc cgctttcgtc tggttgtgaa ggaaagcgat
     1201 cacccctgct ggctcgatga agatgacgaa aacctgcccg tggtgctcga tgccatcgtg
     1261 aaccggggag cacgtttttc gtcggtggaa atgtacctgg tcagtgagtg tattgagcat
     1321 atcctgtcca gtgggctggc ctgcgatgtg ctgcgtatac cggatgaacc gccccgccgc
     1381 tggtttgacc gtggtgttct gcgggaggtg gtccgggaag cccgggccga aatccgcagc
     1441 atggcggatg ccctggcaaa aatccggaaa tgatgacgct ggcggggaaa aatc
//
LOCUS       DAZOXR010000387         1461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZOXR010000387 DAZOXR010000000
VERSION     DAZOXR010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.396"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="ACHUNN_004592"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="ACHUNN_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0578735.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="ACHUNN_004593"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="ACHUNN_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0578736.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          446 a          276 c          337 g          402 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt g
//
LOCUS       DAZOXR010000388         1457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZOXR010000388 DAZOXR010000000
VERSION     DAZOXR010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.397"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHUNN_004594"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHUNN_004594"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0578737.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..>1457)
                     /gene="cadB"
                     /locus_tag="ACHUNN_004595"
     CDS             complement(1090..>1457)
                     /gene="cadB"
                     /locus_tag="ACHUNN_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0578738.1"
                     /translation="LLAAVKMTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYF
                     YSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYAR
                     KMHERQSHSMDNHTASNAH"
BASE COUNT          406 a          320 c          364 g          367 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg attatcaggg cagtcatttt
     1441 cactgcagcc agcagca
//
LOCUS       DAZOXR010000389         1439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZOXR010000389 DAZOXR010000000
VERSION     DAZOXR010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.398"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1231
                     /locus_tag="ACHUNN_004596"
     CDS             <1..1231
                     /locus_tag="ACHUNN_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR0578739.1"
                     /translation="KLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSL
                     AFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKD
                     NILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYT
                     KILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
                     IEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDF
                     CVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTEL
                     NSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKA
                     LREAKSEGGNKVIIHHI"
BASE COUNT          389 a          304 c          282 g          464 t
ORIGIN      
        1 aaaactcatc ccacagatga attacctgat ggttgttgtg gctttgtttt tccttaacgc
       61 cgtcattttt cttttcatgt taatgaaata tttcactaac aaacaaattt taccaacact
      121 cattttaagc cttgcatttt taagtggcct tatctattta gttgaaacca ttgtaattat
      181 ccataaacca attaacggca gtacactgat ccagacaaag tcgaatgatg tttctatttt
      241 ctatattttc cgccaactca gttttatttg tttaacctcg ctggcactct tttgttatgg
      301 aaaagacaac atccttgaca acaataagaa aaaaacggga atcctgttgc tggcgctgat
      361 tcctttttta gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc
      421 tttgtatgtc gtcgactact gtccggacaa ccatactgcg acctggggaa tcaactatac
      481 aaaaatattg gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt
      541 agccagcgaa ctatggccgt taatagcatt attatgtctg gcatcgctat gctgcaactt
      601 acttctactg actctggatg agtataatta caccatctgg tatatcagtc gcgggattga
      661 agtttccagt aaactgtttg ttgtgtcttt tctgatttat aacatttttc agaaactgca
      721 actctccagc aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt
      781 tttcaacagc gtagagtcat tattgtcgag acctgttgtt aaggacttct gtgtcatgct
      841 ggttgatatt aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa
      901 agtgctggtt tcaattgtcg atattatcca gcaaagcatc cgccccgatg atattttagc
      961 gcgactggag ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa
     1021 aatcattgcg gaacgtatgc gtaaaaatgt cgaactcctg accggcttta gtaacagata
     1081 tgatgttcct gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg
     1141 taatatctcg cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg
     1201 gggcaacaaa gtgattattc atcatattta agtgcaaaaa tattcagagc catgcttttt
     1261 gcgtggcttt tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc
     1321 agagatgagt ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct
     1381 cgtttcactg aaccgaagga gtgccattta tcatgaaatt gcaccataga atgctccgg
//
LOCUS       DAZOXR010000390         1431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZOXR010000390 DAZOXR010000000
VERSION     DAZOXR010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.399"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUNN_004597"
     CDS             <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUNN_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0578740.1"
                     /translation="SQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLE
                     IAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDV
                     PLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUNN_004598"
     CDS             464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUNN_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0578741.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAZOXR010000391         1426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZOXR010000391 DAZOXR010000000
VERSION     DAZOXR010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.400"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUNN_004599"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUNN_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0578742.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUNN_004600"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUNN_004600"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0578743.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="ACHUNN_004601"
     CDS             complement(822..>1426)
                     /locus_tag="ACHUNN_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0578744.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAZOXR010000392         1426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZOXR010000392 DAZOXR010000000
VERSION     DAZOXR010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.401"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..655)
                     /locus_tag="ACHUNN_004602"
     CDS             complement(<1..655)
                     /locus_tag="ACHUNN_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR0578745.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
     gene            complement(819..914)
                     /gene="ymiC"
                     /locus_tag="ACHUNN_004603"
     CDS             complement(819..914)
                     /gene="ymiC"
                     /locus_tag="ACHUNN_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0578746.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1028..1195)
                     /gene="yciX"
                     /locus_tag="ACHUNN_004604"
     CDS             complement(1028..1195)
                     /gene="yciX"
                     /locus_tag="ACHUNN_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0578747.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1198..1338)
                     /locus_tag="ACHUNN_004605"
     CDS             complement(1198..1338)
                     /locus_tag="ACHUNN_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0578748.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          382 a          348 c          331 g          365 t
ORIGIN      
        1 tgatcatagt ggtgtgcgag tcagtaccaa cgagtgtatc cggataagca atccattcac
       61 cgtcctgcaa ttcactccac actgctttgc cgagatattc gaggttaacc tgatggcaaa
      121 tgcctgtgcc tggcggcacg acgctaaacc gactgaacgc ttgctttccc catttcagga
      181 acacataacg ttcgtggttg cgctccattt ccaggcgtac gttttcttca aatgcctcat
      241 catcaccaaa acgatcgacg gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg
      301 ggttaacctt tgcagtatcg ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat
      361 caacaacggc aggtacgccg gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa
      421 tttcacggtc agcatgggca tttttcagcc atcctgccag cgcgtggata tcctcttcgg
      481 taaccgagtt accatcctgc cagcgcagca ggttttcgag caaaactttg agtgacttgg
      541 gtagacgggt gatatcgccc agtgatttag cagcaagcgg caggctgtag tagtggtaag
      601 ttttatcttt ggcctgcaac gtgtccttac tggcttctcg tagggttgac gacatagctc
      661 ctccttaatg acagggttgc gaagccctga ttctcttcag ggtgaatatt aaagataaca
      721 caaacatcgc gtaacgattt gataacaacc caaattgata aaagaggaga aaccttgtgg
      781 caaagcaaat gacaaccccg ccgcagcggg gtcaaggatc agtgagtaat gatccagagg
      841 agttcggccc agaagagcat cgacagagaa aacgcgccca tccaggacca atatttcata
      901 tttgtgttga tcataggcta cacccttaac ggttattatt ttgtgttgag ataactcaag
      961 ctgttattgc tgcggtgtat taaaagccct ttaattcatt aaaaatgggt aataagaatg
     1021 atgataatta ttgtttcggc tgatcatctt cagcaaacag ttctataaag gcttgctgct
     1081 gcgatgttag ttgccagtcg tctggaataa cgacggaaca ttcccgtttc gtttctgttt
     1141 cattgtcact atgctggcat aatggtgacg actccagttg ctcctgaccg accatagtta
     1201 tccccacact ttccaaatca gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa
     1261 aaccgccagc caggctttac gttttaattc aggatcgcgg cggggttcct gatttccaga
     1321 aggcattgct aacctcatat aatcgccatc acttatcagc aagacgttga acgattggtg
     1381 taattaatca tacgatgaga caaatcctaa agaaacttta gtcaaa
//
LOCUS       DAZOXR010000393         1422 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZOXR010000393 DAZOXR010000000
VERSION     DAZOXR010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.402"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHUNN_004606"
     CDS             complement(<1..188)
                     /locus_tag="ACHUNN_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0578749.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            286..546
                     /locus_tag="ACHUNN_004607"
     CDS             286..546
                     /locus_tag="ACHUNN_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578750.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            543..1133
                     /locus_tag="ACHUNN_004608"
     CDS             543..1133
                     /locus_tag="ACHUNN_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578751.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(1153..>1422)
                     /locus_tag="ACHUNN_004609"
     CDS             complement(1153..>1422)
                     /locus_tag="ACHUNN_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR0578752.1"
                     /translation="PLLRILWRLGIRLPPLPFMPFWQVTVLTGGLWGISWGCAMWFIY
                     WGPSGMVAGEAIIISITGGFLSGLLMASFHWWRRKVNRLPPWDDV"
BASE COUNT          332 a          407 c          413 g          270 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggagc aatggaacaa attgtcgccc attgtgagtc atattcgccc
      121 tgactttcca gaaccatacg gactgcccgt tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcagga agtttagtct ccaggattcc cggggcggtt
      241 caatgatttt tacctgtcag aacattatca gatggaacgt gcaggatgga tcaggaaatg
      301 acgttttcac tcagctatga acaactgacc cggtttgcgg aaaaacgcat ccgggaatgc
      361 aatctggaca gccacggcgt aacttatctc tgcgagtcag ccaaagccgg agccgtgctg
      421 atcttctggc atgaactggc cataaatggc tatacctcaa tgaatgccat aaagagacag
      481 gagatcattg acgccgacca tcagcgcctc agaaagctca tctggccgga ggatgactgg
      541 aaatgactca gagccgccga ccgtcgccac tgcagcggag agtgctgatt gtgctggctg
      601 cccttgatga aaaacggcca ggacccgtgc tgacgcggga catagaacgg gtgctggagc
      661 aaagcgggga agccccggtc tacgggccaa acctgcgcgc ctcctgccgt cgtctggagg
      721 atgccggctg gctgcgtacc ctgcgcgctc cgaacctgca gctggcagtg gagctgacgg
      781 acgccggccg ggccgtggca cagcctctgc tgctggcaga acaggaccgc atccgggcag
      841 aacagcgtgc ggcagaagtc gtcgtactgc cgctggtccc ggcagcaggt ctgcccgcag
      901 gcggcacttc tgccactgat ctggcggtgg agctgaacgg cataacgtat caggcctgcc
      961 gtggggattt tgtggtgcgt ctcgacggca gcacctgtct gcagttatgg aataaagaag
     1021 gctgggtggt acgccgggag ggcgatccgc tggaagtggc gcagtggctg ccggcctgtc
     1081 acgatgcagg aatggaggtt cgcgtacaga taaatgaaag tgccgctccg taagaacacg
     1141 cctgtgcacg gtttacacat catcccatgg tggcagtcgg ttaactttcc tgcgccacca
     1201 gtggaaagag gccatgagca gtccggacaa aaaacccccc gtgatactta taataatggc
     1261 ctcacctgcc accattcctg acggccccca gtagataaac cacattgcac atccccagga
     1321 aatgccccat aagccccccg tcagcaccgt gacctgccag aacggcataa agggtaatgg
     1381 cggaagccgg atacccagcc gccagagaat acgcagaaga gg
//
LOCUS       DAZOXR010000394         1420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZOXR010000394 DAZOXR010000000
VERSION     DAZOXR010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.403"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHUNN_004610"
     CDS             <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHUNN_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR0578753.1"
                     /translation="VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAH
                     RHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGL
                     KRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          421 c          436 g          225 t
ORIGIN      
        1 tggtactgac cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg
       61 ggacggaacc gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca
      121 acggcatccg tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac
      181 ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg
      241 accgcagcaa tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg
      301 tggcgcaccg tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg
      361 tgacagaaca gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca
      421 tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc
      481 tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg
      541 cggtgggcag gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg
      601 atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
      661 actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc
      721 gcaaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc
      781 gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca
      841 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg
      901 gttatgtaac ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg
      961 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
     1021 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
     1081 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca
     1141 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
     1201 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacctg ctgatacagg
     1261 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc
     1321 gcagcgagga tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc
     1381 accgcaccgt gaatggcgaa accgcagagc ggtggcagta
//
LOCUS       DAZOXR010000395         1400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZOXR010000395 DAZOXR010000000
VERSION     DAZOXR010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.404"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHUNN_004611"
     CDS             <1..207
                     /locus_tag="ACHUNN_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HFR0578754.1"
                     /translation="WGTIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVES
                     PAGSRKWYKYEHKTGCIASCVTLN"
     gene            complement(204..452)
                     /locus_tag="ACHUNN_004612"
     CDS             complement(204..452)
                     /locus_tag="ACHUNN_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578755.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(1120..>1400)
                     /locus_tag="ACHUNN_004613"
     CDS             complement(1120..>1400)
                     /locus_tag="ACHUNN_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFR0578756.1"
                     /translation="KSNSGSTVITGRIANGQQIPLPTGFSASQCSWSVSNAENPQGWK
                     PNYFAGSVATYDANRIVKCGFYDEYNFHKGTFRADLTGKCSYVVACQN"
BASE COUNT          391 a          332 c          302 g          375 t
ORIGIN      
        1 tgggggacaa taggtggaaa actcaaagtt actcagcttt ccaccacagg ttatctgggg
       61 caattcgact tctgtgccat tgccagaatg ggcaacgcag aggatgccca ctactgccag
      121 gtagttgaga gcccagcagg ttcacgcaaa tggtacaaat acgagcataa gacaggttgt
      181 attgcgtcgt gtgtaacgct caattaagtt tgatatccaa atactgaaaa taccccggca
      241 ccacatgatg tattttctgt tggataggat gttatctgat aggaagtacc tgcaggtaca
      301 gcaaagctga taaaggctgt ttttccatat gcagggttat tgctggcatt cacgctaatt
      361 agcgtcccac caacatatcc ctgcagtcgg gatgtgtttg cacatgcacc tccggcagat
      421 cctccattac ctccagatgc ataaataaac aatgtactgc cccctgaatt cctccctgag
      481 aatgaacctc tatgtgaacc taagtttgtg taagaaccat tgagcgaacc agaagtcttc
      541 cacgtaccgg attggcacgt aggccagtgc caatccggta cctggaaatc atcatcagcg
      601 tcgatatgga cgaatataaa aacatttacg ttatatccca aaaacacaca ggtcttaggg
      661 aggtttaaac tgtgtattaa cacctatcgt attgacgggc gtgaaatggc agaaacagaa
      721 gtcgttccta ttgacatgcc tgactctaac ggcgagatga cttggcaagc caaaaactac
      781 acacagtatt cgtcctactt tatgaagatt acttgcttga aatagcttat tggcaaatgg
      841 catatacagt tattgaacca ttctgatctt cggttgtatc gcacgcagcc gaccatgagt
      901 tattggctga tgggaatgac ctggtaagcc atatgtaacc agttcgtgag gtgcatattc
      961 cgccgccacc tgtaacacgt tttccagttg gacaggtagc tgttgctcca ggaaagcgcc
     1021 aacctgtggc ctgtgctgta gcaatttgaa gctcaccaga tcctaccttt ctccaggtac
     1081 cggattggca cgtaggccag cagcgccagc aatttaatct cagttctgac acgcgacgac
     1141 ataactacat tttccggtta aatcagctct aaatgtccct ttatggaaat tgtattcatc
     1201 ataaaaacca catttaacaa ttcgatttgc atcataagtt gcaactgatc ctgcaaagta
     1261 attcggcttc caaccttgag ggttttctgc attgctcaca ctccatgagc actgactggc
     1321 agaaaatcct gttggcagcg gtatttgctg accatttgct atacgaccag ttatcaccgt
     1381 actaccagaa ttgcttttac
//
LOCUS       DAZOXR010000396         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZOXR010000396 DAZOXR010000000
VERSION     DAZOXR010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.405"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUNN_004614"
     CDS             <1..459
                     /locus_tag="ACHUNN_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0578757.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUNN_004615"
     CDS             477..1115
                     /locus_tag="ACHUNN_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0578758.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZOXR010000397         1388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZOXR010000397 DAZOXR010000000
VERSION     DAZOXR010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.406"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHUNN_004616"
     CDS             complement(<1..185)
                     /locus_tag="ACHUNN_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0578759.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHUNN_004617"
     CDS             complement(230..1216)
                     /locus_tag="ACHUNN_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0578760.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          384 a          302 c          280 g          422 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaaccta tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1381 gtttataa
//
LOCUS       DAZOXR010000398         1376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZOXR010000398 DAZOXR010000000
VERSION     DAZOXR010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.407"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..983
                     /locus_tag="ACHUNN_004618"
     CDS             <1..983
                     /locus_tag="ACHUNN_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0578761.1"
                     /translation="VVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="ACHUNN_004619"
     CDS             998..>1376
                     /locus_tag="ACHUNN_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0578762.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAZOXR010000399         1352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZOXR010000399 DAZOXR010000000
VERSION     DAZOXR010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.408"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..485)
                     /gene="umuC"
                     /locus_tag="ACHUNN_004620"
     CDS             complement(<1..485)
                     /gene="umuC"
                     /locus_tag="ACHUNN_004620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0578763.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQT
                     GG"
     gene            complement(485..904)
                     /locus_tag="ACHUNN_004621"
     CDS             complement(485..904)
                     /locus_tag="ACHUNN_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0578764.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1133..>1352
                     /locus_tag="ACHUNN_004622"
     CDS             1133..>1352
                     /locus_tag="ACHUNN_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578765.1"
                     /translation="MFDIYHINRNKDIDASRPANYELDEVKFINSCKTGVSLQFIYLK
                     RRMIMTEIVADKTVEVVKNAIETADGALD"
BASE COUNT          405 a          327 c          278 g          342 t
ORIGIN      
        1 ccccccgtct gccgctgcca tttttttgcc gcatgattgg caagcttagc cagcgttttg
       61 gtctgggcga tccccatacc aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt
      121 tctctgccaa aatcagtcag atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc
      181 tcatcaatac tgtaaatctc gacgcggggc gatagttctt ccagcgtcga catcacccga
      241 ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc aaaccacacc acagcgacga
      301 aacagatctt tttgtttgaa ccagggatcg cccattttaa cacccagcgc ctttgcctca
      361 gcgtttcggg cgataacgca accgtcatta ttcgatagca caaccaccgg tttaccccat
      421 aaatcagggc gaaacaccgt ctcacagctg gcataaaacg cgtttacatc acagagagca
      481 aacatcagcg catcgcctta acgacgtgga tcaccacacc aaagacatcc agcgtatctt
      541 cgctactgat ggtaatgggc gagtacgcac tgttcatggg aataagctgt accgtcgggc
      601 gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca atatcaccat
      661 ggctggcggt aatagcgcta tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa
      721 tcatagaatc accacttgct ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt
      781 gattcagatc gatgcgctgt tcgacgtaat ctgctgccgg tgaaggaaag ccacactgaa
      841 caagatcgct aaatagcgga aaagtcacaa tttcgcggag atccgcaggc ttgataaaca
      901 acataataat ctgcctgaag ttatactgtt tttatataca gtaatctgtt cttgccagca
      961 gatcaatact gattcaggct atcaatattt gtcgctgcat agactgctga tttttcgttc
     1021 tcttatcctg tgctcacgtg gccttctggc gacgacgctc atccagcaga aatgaaaaat
     1081 atcacccggc aaaaaaatag aatagaagca ttcgccataa tgacattaaa cattgtttga
     1141 tatttatcat attaatagaa ataaagacat tgacgcatcc cgcccggcta actatgaatt
     1201 agatgaagta aaatttatta atagttgtaa aacaggagtt tcattacaat ttatatattt
     1261 aaagaggcga atgattatga ctgaaatcgt tgcagataaa acggtagaag tagttaaaaa
     1321 cgcaatcgaa accgcagatg gagcattaga tc
//
LOCUS       DAZOXR010000400         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZOXR010000400 DAZOXR010000000
VERSION     DAZOXR010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.409"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUNN_004623"
     CDS             <1..184
                     /locus_tag="ACHUNN_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578766.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUNN_004624"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUNN_004624"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0578767.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZOXR010000401         1294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZOXR010000401 DAZOXR010000000
VERSION     DAZOXR010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.410"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..1112
                     /locus_tag="ACHUNN_004625"
                     /pseudo
     CDS             1..1112
                     /locus_tag="ACHUNN_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1090..>1294
                     /locus_tag="ACHUNN_004626"
     CDS             1090..>1294
                     /locus_tag="ACHUNN_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578768.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAZOXR010000402         1288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZOXR010000402 DAZOXR010000000
VERSION     DAZOXR010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.411"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..1175)
                     /locus_tag="ACHUNN_004627"
     CDS             complement(<1..1175)
                     /locus_tag="ACHUNN_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0578769.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     "
BASE COUNT          420 a          244 c          188 g          436 t
ORIGIN      
        1 ccgttttcaa aacaacattt tttcaatatg ctttcattca caacagtgtg tgataagtag
       61 agattactta tgttttgctt tattaatgcc acgtcattaa aatgactata tttaatgaaa
      121 aaaccatcat tgagtagtgt tttaagattg actaaattac ttgcatcttc acgtaccatc
      181 gtggcaactg gaacaggcag agacatgaaa acttcttcat tcaccatcaa gtgaatcatg
      241 cattgcgttc cctttgtcat atccacacat acgctacaaa tatcgtggta atgcgaagag
      301 cgcatttcag gaaacagatt aaacacggta tcttgaaagg cactaaatcg tgttaataaa
      361 tcctgataat gaacaggttt tgtttcaaaa ttatcatcag caaatccaag cactattgta
      421 tgctctacat ctaacgcagt ttcaaatggc tcttcttctg taaaatcatt atttaaaacc
      481 tcacctgacg tattatcact gtcataaaaa gtgttcatca atgcgatatt gtttgttaca
      541 gcgtcttttt cgatattctc actaattgca tatcgcctgc tataatcata aaaatgaata
      601 aacgcgccct ttataaagga tatatattgt tctttgttaa agtcaatacg gctgtataat
      661 ttttcaaaaa tcacattcgc aattttacta ccaatggtat gcctgtataa taatgggtgc
      721 gattcaatcg taaaatgctt gtctaattga tcttgtaatt tattctcata gtccagaggg
      781 aaaagaaact gttgtctttc aagtttccca ttcgatacat tacgtattcc aatagagatt
      841 ctttcattac ccgcctttat ttcaacttcg ttatcttctg atttacccag atatttttta
      901 acatcagtct cgaaagcatt actaaaaaaa gtattaaatt ggtaattcgc tgatggcata
      961 ttttccgcga gcaacacatc attttttatg agatcactgt cagcatttat tgctaatatc
     1021 agatgcaagt agatggtatc ccatgtagac aactcaccct tacgactgga atttaatatc
     1081 gcgaaaaatt ccctcagtga atccaggaaa ttatcacttt gatttagttg tgtgcctgga
     1141 aatgatgatg cttgatcaga tatattgttt atcatattaa aaatcaccca agaaaaacga
     1201 taatccaccg aactcctcgt gctttataat tataaaagca caacgccttt tatattcata
     1261 ttaattaacg agatactgaa acaactta
//
LOCUS       DAZOXR010000403         1285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZOXR010000403 DAZOXR010000000
VERSION     DAZOXR010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.412"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            114..1196
                     /locus_tag="ACHUNN_004628"
     CDS             114..1196
                     /locus_tag="ACHUNN_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0578770.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          377 a          272 c          279 g          357 t
ORIGIN      
        1 acaaatcaaa cgagaaacca aaacttacat cttgaaataa tcacattgat tagatgaata
       61 tttatcgcgc aatgacatca ttttttaata atagttcaaa aaaagggctc acgatgaaaa
      121 aattaacagt ggcaatttct gctgtagctg catcagtact gatggcgatg tctgctcagg
      181 cagctgaaat ttataataaa gacagtaaca agctggatct gtacgggaaa gttaatgcta
      241 agcactactt ctcctctaat gatgcagatg atggtgatac tacttatgcc cgtcttggct
      301 tcaaaggtga aacccaaatc aacgatcaac tgactggttt cggtcagtgg gaatatgaat
      361 tcaaaggcaa ccgcgctgaa tctcaaggtt cctccaaaga taaaacccgt cttgccttcg
      421 ctggcctgaa attcggtgac tacggctcca tcgattacgg ccgtaactac ggtgtagcat
      481 acgacatcgg tgcgtggact gacgtcctgc cagaattcgg tggtgacact tggactcaaa
      541 cagatgtgtt catgactggt cgcaccactg gtgttgcaac ttatcgtaac aacgacttct
      601 ttggtctggt tgatggtctg aactttgctg ctcagtatca gggtaaaaat gaccgcactg
      661 acgtaactga agctaatggt gatggtttcg gtttctccac tacttatgag tatgaaggat
      721 tcggtgtagg tgcaacctat gctaaatctg accgcactaa taatcaggtt atctacggta
      781 acaacagcct gaatgcatct ggtcaaaatg ctgaagtatg ggcagctggt ctgaaatatg
      841 atgcgaacaa catctatctg gctaccacct attctgaaac ccagaacatg actgtttttg
      901 gtaataacca tattgccaac aaagcacaaa acttcgaagt agttgcacaa tatcagttcg
      961 acttcggtct gcgtccgtcc gttgcttacc tgcaatctaa aggaaaagac ttgggtgcgt
     1021 ggggtgatca ggacctggtt gaatatattg atgtaggtgc aacctattac ttcaacaaaa
     1081 atatgtccac ttttgttgat tacaaaatca acctgattga taagagcgat ttcacgaaag
     1141 catctggcgt tgctaccgat gatatcgttg ctgtaggtat ggtttaccag ttctaatttg
     1201 attactaaaa gatatgttgc gggaggcttt gcctccccaa catataagtg gctccctcaa
     1261 gccacttcct ttaggagcac aacct
//
LOCUS       DAZOXR010000404         1264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZOXR010000404 DAZOXR010000000
VERSION     DAZOXR010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.413"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(23..>1264)
                     /locus_tag="ACHUNN_004629"
     CDS             complement(23..>1264)
                     /locus_tag="ACHUNN_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0578771.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          333 a          298 c          228 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtcg ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAZOXR010000405         1247 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZOXR010000405 DAZOXR010000000
VERSION     DAZOXR010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.414"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(56..1000)
                     /gene="leuO"
                     /locus_tag="ACHUNN_004630"
     CDS             complement(56..1000)
                     /gene="leuO"
                     /locus_tag="ACHUNN_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0578772.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          380 a          285 c          250 g          332 t
ORIGIN      
        1 caataaaaaa tacagaataa accagacatt catgtctgac ctattctgca ttcagttagc
       61 gtttgcaaat tgagactaat tgctcttcca tccactgatg gcctttatcg cgcccggcag
      121 cttcatgcca ggagagataa caggttctgc tattttgttt taacggcagc ggtaatacct
      181 gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata gcgaccaaat
      241 gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac gcgatactgg
      301 cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga tcgagcgaaa
      361 ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccctta attgttggat
      421 gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg gtaaattcag
      481 gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc agctgatgtt
      541 cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca atctgctcaa
      601 tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca caaagatgaa
      661 atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt tgtactagtt
      721 gcaatgcctg acgaactgag ccaaaaagtt gaaatgcgcg agcagtcggt tgaataccac
      781 ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca acagcgttac
      841 tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt tgctcctgca
      901 tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt ggcttgctta
      961 actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc actgtcacac
     1021 ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat gatttatcat
     1081 gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag atacgggttc
     1141 tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata agaatccata
     1201 atgtttttgt tgcgaaaaca atctaatcat aactaccgcg aatactc
//
LOCUS       DAZOXR010000406         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZOXR010000406 DAZOXR010000000
VERSION     DAZOXR010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.415"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHUNN_004631"
     CDS             complement(<1..680)
                     /locus_tag="ACHUNN_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0578773.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="ACHUNN_004632"
     CDS             complement(996..>1211)
                     /locus_tag="ACHUNN_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0578774.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAZOXR010000407         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZOXR010000407 DAZOXR010000000
VERSION     DAZOXR010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.416"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            54..1211
                     /locus_tag="ACHUNN_004633"
                     /pseudo
     CDS             54..1211
                     /locus_tag="ACHUNN_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          273 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca g
//
LOCUS       DAZOXR010000408         1207 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZOXR010000408 DAZOXR010000000
VERSION     DAZOXR010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.417"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..392)
                     /locus_tag="ACHUNN_004634"
     CDS             complement(<1..392)
                     /locus_tag="ACHUNN_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR0578775.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDK"
     gene            complement(409..>1207)
                     /locus_tag="ACHUNN_004635"
     CDS             complement(409..>1207)
                     /locus_tag="ACHUNN_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0578776.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          318 a          267 c          239 g          383 t
ORIGIN      
        1 ttcttatctt ttattttatt tactgtttca atgcaatcag cggcaagttg gagtggattt
       61 agatttttca gatatggtct tatgatcttt cccttactta ataaatagca tgtataggat
      121 tcggaaagtc cagcatctat tttgtacctc attatattag ataaggatgt gagaaattcc
      181 ttgttatcat tcgcagccat atatgccagt ccgagttcag cctctgcggc cttacaggca
      241 aaatagctta gtgaacacgc ttttcgtccc tctttagtac tggctgatat tagtgcccat
      301 aatattgctt tgtctgccaa atctaactct ctctgaaatt gtcttgcagt aactgcactc
      361 gcagaaatag agaaaaaaat acaattgtta ataactttgc catatttttt agtccgtaac
      421 tgttaatgtg ctatctggct ctgaaaatat taactcattt aatttttgca tgtcattaga
      481 tgaaccagtt atacaaccct cggaacagaa tcgaggtcct aaagacggac cgaaagcgtt
      541 taaacaactt tgtgttaaaa atccatcacg attatatgtt tctgtatttg ctgacggtac
      601 taaacgtata ccattaggtt ttctattggt tgctccagga ccattaacat tccagctcca
      661 taccccttga ggtattggac ctctattttg taagtgtgta caaccaggat tatttttaca
      721 ttgcatatta ttaccattat taccagaggc gacaggaata gtaagcacat cataagagtt
      781 atcatcattt tgcgtatctg aaacgcatga aagcatactt aaagttatgc tgtataaaca
      841 ggtcgctaat cccaacgggt caatatcagt tatcggattc aacggatact gataaaaatt
      901 ccatcccccc ttcagtccaa tcggatcctg agtgatatat cgcccctgca gcgggtcata
      961 atagcggtgg cggttgtaat acaggccgga ctcctcatca tactgctgcc ccggcaggcg
     1021 gataagctgc tgcagctgat gcgggttctc ttcattcagc aggttgcccc actcatcgta
     1081 ttctgcgcac cattctgttg ccccttccgt gctgataagc accagcggca ggccgcgatg
     1141 gtcgcagtgg tacaggtgga tttttcgcgc cggcgtgtac accgggtcca tctggttttg
     1201 catctgc
//
LOCUS       DAZOXR010000409         1199 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZOXR010000409 DAZOXR010000000
VERSION     DAZOXR010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.418"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            168..686
                     /locus_tag="ACHUNN_004636"
     CDS             168..686
                     /locus_tag="ACHUNN_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0578777.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            896..>1199
                     /locus_tag="ACHUNN_004637"
     CDS             896..>1199
                     /locus_tag="ACHUNN_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0578778.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          289 a          306 c          311 g          293 t
ORIGIN      
        1 atgattttaa aaatagtttt tacgacaacc gtaaattcta gctcggtttg tatgtaaaaa
       61 atactgaaaa atgatgcggt tccgtgaagt gagctgcatc tgcctgtctg ggattaacgg
      121 caggtcattg taagtagtaa ttaaccacgt taacaaggag tttacaaatg ccaacaccgt
      181 gttatatctc catcactggt cagacccagg gaaatattac tgcaggtgca tttaccgctg
      241 attctgtcgg caacatctat gtgcaggggc acgaagacga gatgctggtg caggaattcc
      301 tgcataacgt tactgtcccg accactcctc agtccggtca gcctgcgggt cagcgtgctc
      361 acaagccttt catttttacc gtggcgctga acaaagcggt tccgctgatg tacaacgcac
      421 tggcctccgg cgaaatgctg ccaaccactg aacttcactg gtggcgtacc tctgtggagg
      481 gcaaacagga gcattatttc actacccgcc tgactgattc gacgattgtc gatatgaaac
      541 tgcatatgcc gcattgccag gacccggcga aacgcgagtt cacccagttg ctcgaggttt
      601 cactggccta ccgcaaaatt gaatgggaac acgtcaaatc tggcacctcc ggtgccgacg
      661 actggcgcgc accactggaa gcataagtta agccaacagc atccggctat caggccggat
      721 gctgtttttg tattacgccg tagaaagaaa ggttgtcagt aaggggttac gttcacggga
      781 gatggcggat gccggaaact tcggcggtag cgtgcctgaa cgcaagcgta acactgtact
      841 gtcacagccg ctttaacagc gaactgtctg tttttcctta accccggagg ttgctatgtc
      901 aaccggatta cgtttcacgc tggaagtgga cggcctgcca ccggatgctt ttgcggtggt
      961 ctcctttcat ctgaaccagt cactctcttc gctcttttcc ctcgagctct ctctggtcag
     1021 ccagcagttt ctctcccttg aatttccgca ggtactggac aaaatggcct acctgacgat
     1081 atggcagggc gatgacgtac agcgccgggt gaaaggtgtg gtgacctggt ttgaactggg
     1141 ggagaacgac aaaaaccaga tgctgtacag catgaaggta tgcccaccgc tgtggcgca
//
LOCUS       DAZOXR010000410         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZOXR010000410 DAZOXR010000000
VERSION     DAZOXR010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.419"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            223..1038
                     /gene="yahL"
                     /locus_tag="ACHUNN_004638"
     CDS             223..1038
                     /gene="yahL"
                     /locus_tag="ACHUNN_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR0578779.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          364 a          230 c          233 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagc
//
LOCUS       DAZOXR010000411         1194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZOXR010000411 DAZOXR010000000
VERSION     DAZOXR010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.420"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..689
                     /locus_tag="ACHUNN_004639"
     CDS             <1..689
                     /locus_tag="ACHUNN_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0578780.1"
                     /translation="SQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
     gene            853..>1194
                     /locus_tag="ACHUNN_004640"
     CDS             853..>1194
                     /locus_tag="ACHUNN_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0578781.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSV"
BASE COUNT          355 a          258 c          238 g          343 t
ORIGIN      
        1 ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact
       61 acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg
      121 aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt
      181 tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat
      241 ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga
      301 atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta
      361 attacgctga aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac
      421 aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt
      481 acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg attgagtata
      541 tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa
      601 tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc gataacatcg
      661 tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct
      721 ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat
      781 ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat
      841 atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt
      901 tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga
      961 ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag
     1021 cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg
     1081 agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta
     1141 acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag cgtt
//
LOCUS       DAZOXR010000412         1190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZOXR010000412 DAZOXR010000000
VERSION     DAZOXR010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.421"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>1190
                     /locus_tag="ACHUNN_004641"
     CDS             <1..>1190
                     /locus_tag="ACHUNN_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0578782.1"
                     /translation="AEQEALIHALQEKLSNREREIDHLQAQLDKLRRMNFGSRSEKVS
                     RRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAP
                     CCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERG
                     IAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEAL
                     HGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDR
                     KGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALT
                     EEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELA
                     KAFA"
BASE COUNT          265 a          339 c          375 g          211 t
ORIGIN      
        1 gccgaacagg aagcgctgat ccacgccctg caggaaaagc ttagcaaccg ggagcgcgaa
       61 atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg cagtcgttcc
      121 gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct tcagaaagag
      181 agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt gcgtcagacc
      241 cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact gttgcctgcg
      301 gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga taccgccgaa
      361 cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga aaaacatgcc
      421 tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat cgagcggggt
      481 atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga gcacaccccg
      541 ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg ttcactgctg
      601 tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc gcttcatggc
      661 tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt gctgttgcca
      721 ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga ccgtaacgcc
      781 gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa aggcatccat
      841 ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata cgccgggttc
      901 aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca cgcccgccgt
      961 aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc gctgaaacgg
     1021 atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga acagcgcctt
     1081 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
     1141 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZOXR010000413         1171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZOXR010000413 DAZOXR010000000
VERSION     DAZOXR010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.422"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            201..>1171
                     /gene="cadB"
                     /locus_tag="ACHUNN_004642"
     CDS             201..>1171
                     /gene="cadB"
                     /locus_tag="ACHUNN_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0578783.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT          257 a          254 c          288 g          372 t
ORIGIN      
        1 tcatgatatc aacttgcgat cctgatgtgt taataaaaaa cctcaagttc tcacttacag
       61 aaacttttgt gttatttcac ctaatcttta ggattaatcc ttttttcgtg agtaatctta
      121 tcgccagttt ggtctggtca ggaaatagtt atacatcatg acccggactc caaattcaaa
      181 aatgaaatta ggagaagagc atgagttctg ccaagaagat cgggctattt gcctgtaccg
      241 gtgttgttgc cggtaatatg atggggagcg gtattgcatt attacctgcg aacctagcaa
      301 gtatcggtgg tattgctatc tggggttgga ttatctctat tattggtgca atgtcgctgg
      361 cgtatgtata tgcccgactg gcaacaaaaa acccgcaaca aggtggccca attgcttatg
      421 ccggagaaat ttctcctgca tttggttttc agacaggtgt tctttattac catgctaact
      481 ggattggtaa cctagcgatt ggtattaccg ctgtatctta tctttccacc ttcttcccag
      541 tattaaatga tcctgttccg gcgggtatcg cctgtattgc tatcgtctgg gtatttacct
      601 ttgtaaatat gctcggcggt acctgggtaa gccgtttaac cactattggt ctggtgctgg
      661 ttcttattcc tgtggtgatg actgctattg ttggctggca ttggtttgat gcggcaactt
      721 atgcagctaa ctggaatact gcggatacca ctgatggtca tgcgatcatt aaaagtattc
      781 tgctctacct gtgggccttc gtgggtgttg agtccgcagc agtaagtact ggtatggtta
      841 aaaacccgaa acgtaccgtt ccgctggcaa ccatgctggg tactggttta gcaggtattg
      901 tttacatcgc tgcgactcag gtgctttccg gtatgtatcc gtcttctgta atggcggctt
      961 ccggtgctcc gtttgcaatc agtgcttcaa ctatcctcgg taactgggct gcgccgctgg
     1021 tttctgcatt caccgccttt gcgtgcctga cttctctggg ctcctggatg atgttggtag
     1081 gccaggcagg tgtacgtgcc gctaacgacg gtaacttccc gaaagtttat ggtgaagtcg
     1141 acagcaacgg tattccgaaa aaaggtctgc t
//
LOCUS       DAZOXR010000414         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZOXR010000414 DAZOXR010000000
VERSION     DAZOXR010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.423"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHUNN_004643"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHUNN_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR0578784.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1161)
                     /locus_tag="ACHUNN_004644"
     CDS             complement(510..>1161)
                     /locus_tag="ACHUNN_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0578785.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          276 g          257 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cgggggtcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa t
//
LOCUS       DAZOXR010000415         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZOXR010000415 DAZOXR010000000
VERSION     DAZOXR010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.424"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUNN_004645"
     CDS             <1..122
                     /locus_tag="ACHUNN_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578786.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHUNN_004646"
     CDS             119..376
                     /locus_tag="ACHUNN_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578787.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHUNN_004647"
     CDS             376..750
                     /locus_tag="ACHUNN_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0578788.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHUNN_004648"
     CDS             747..>1148
                     /locus_tag="ACHUNN_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0578789.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZOXR010000416         1142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZOXR010000416 DAZOXR010000000
VERSION     DAZOXR010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.425"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUNN_004649"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUNN_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1142
                     /locus_tag="ACHUNN_004650"
     CDS             572..>1142
                     /locus_tag="ACHUNN_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0578790.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLICLCY
                     SQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSS
                     VSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLV
                     TEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          319 a          255 c          271 g          297 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctaat ctgtctgtgt tactctcaga tatccctggc
      721 gggtattgtc cgttccgata ttgcctatca gatttatcgt gatttcgccg aaaacaaagg
      781 gctttttgta cctggtgcca atgatattcc ggtatatgat aaggacggaa aacttgtggg
      841 aagactgggt aaagccccaa tggccgattt cagcagtgtg agctcaaatg gcgttgctac
      901 gcttgtatcg cctcagtata tcgtcagcgt aaagcataac ggaggatatc ggagtgtgag
      961 ctttggtaat gggaaaaata catattccct tgttgaccgt aataaccacc cttctattga
     1021 cttccatgct ccacgtctga ataaactggt tacagaagtt attccctcag cggtaacatc
     1081 agaaggaacc aaagccaatg cttataaata cactgaacgt tacaccgctt tttatcgggt
     1141 gg
//
LOCUS       DAZOXR010000417         1137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZOXR010000417 DAZOXR010000000
VERSION     DAZOXR010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.426"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>1137
                     /locus_tag="ACHUNN_004651"
     CDS             <1..>1137
                     /locus_tag="ACHUNN_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFR0578791.1"
                     /translation="YRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVL
                     DSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMA
                     SPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLD
                     EMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYI
                     ENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVD
                     FDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQ
                     WFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEG"
BASE COUNT          263 a          298 c          322 g          254 t
ORIGIN      
        1 ctaccgtaac cagctggcgt ttatcgatat tttcttccgg ggaatgaata acaccaacta
       61 caacatggcg gtctgtggca cctccggggc cggtaaaacc gggctgatac agccacttat
      121 ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc gacatggggg atggctacaa
      181 gtccctgtgt gagaacatgg gtggcgtata tctggacggt gaaaccctgc gttttaaccc
      241 gtttgcgaac atcaccgata tcgaccagtc agcggagcgt gtccgtgacc agttgtcagt
      301 gatggccagc cccaacggta acctggatga agtgcatgaa ggtctgctgc tgcaggcggt
      361 cagggcctcc tggctggcca aagagaacag ggcacgtatt gatgacgtgg tggatttcct
      421 gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg actatccgca gccgtctgga
      481 cgaaatgatt gtgctgcttg accagtacac tgccaacggc acttacggcc agtattttaa
      541 ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg gtgctggagc tgggcggact
      601 ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc ctgattatct acatcgagaa
      661 caggatgtac cgcacgccgc gtaacctcaa gaaactgaac gtcattgatg aaggctggcg
      721 tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt gagaaaggtt accgtaccgc
      781 ccgtcgtcat accggtgctt atatcaccat cacacagaac atcgtcgact ttgactctga
      841 caaggcctcc agtgcggccc gtgcggcatg gggtaactcc tcctacaaaa ttatcctcaa
      901 acagagtgcg aaggagttcg caaaatacaa ccagctgtat ccggaccagt tccagccact
      961 gcagcgcgac atgattggta agtttggtgc ggccaaagac cagtggttca gttccttcct
     1021 gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt gtggacccgt taagccgcgc
     1081 catgtacagc tctgacggcc cggattttga gtttgtgcag cagaaacgta aagaggg
//
LOCUS       DAZOXR010000418         1126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZOXR010000418 DAZOXR010000000
VERSION     DAZOXR010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.427"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUNN_004652"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUNN_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR0578792.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..>1126
                     /locus_tag="ACHUNN_004653"
     CDS             1017..>1126
                     /locus_tag="ACHUNN_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0578793.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKV"
BASE COUNT          340 a          221 c          268 g          297 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtaga
//
LOCUS       DAZOXR010000419         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZOXR010000419 DAZOXR010000000
VERSION     DAZOXR010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.428"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHUNN_004654"
     CDS             84..1115
                     /locus_tag="ACHUNN_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0578794.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZOXR010000420         1094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZOXR010000420 DAZOXR010000000
VERSION     DAZOXR010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.429"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            180..965
                     /locus_tag="ACHUNN_004655"
     CDS             180..965
                     /locus_tag="ACHUNN_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578795.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          362 a          187 c          228 g          317 t
ORIGIN      
        1 tcatactatc aaatcattca atttaaatat atttgaatgt tatgattttc atttttctat
       61 tgatttaatg aatttatata tactgttatt tttgtttgtt ctgattcaag tggagtatat
      121 aattaaaaat cgttagctaa acattaccat gattatgaca ggcgagggag tgtccgatta
      181 tgaatttaag aagggtttgt tgtatatgtg tattattctc gctgcttgca ggatgtgtct
      241 ctgaatcttc tattgatgaa aagaagaaaa aagctcaagt cacacaaagt aatattaata
      301 aaaacactcc ccagcaactg acagacaaag atttattcgg taatgaaacc actctggccg
      361 tatccgaaga ggatattcaa gctgcgcttg atggagatga gttccgcgtt ccccttaatt
      421 ctcctgtaat acttgttcaa tccggcaacc gcgcaccgga aaccattatg caggaggaga
      481 tgcgaaaata ttatactgtt tccacattct ctggtatccc ggacaggcaa aagcctctga
      541 cttgtaacaa aaacaaagat aaaaatgaga acgaggatgt tgccagtgct gagaatatga
      601 actggatgca ggcactgcgt tttgtggctg caaaagggca tcagaaagcg attattgttt
      661 tccaggatac gttgcagaca ggaaaatatg actctggatt gaaatcaata gtttggtcag
      721 attataaaaa tcaaaaattg actgacacga tgtcactgcg ctatctggta cgattcacgc
      781 tggtggatgt agctacaggt gagtgggctg cctggtcgcc ggtgaattat gaatataaag
      841 tgcttccacc attgcccgac aagaatgaag ccagtacgac tgatatgaca gagcagcaaa
      901 tcatgcaact taagcaaaag acctataaag cgatggtaaa agatttggtt aaccgctatc
      961 aataataaat tatatctgcc gccaggaatt tctggcggaa ataatacaaa attctttgca
     1021 taagaaattt ttcttgacaa agacagtgca agaaaggaca tggaatccgg agttaaaaac
     1081 tgtttgtttt agaa
//
LOCUS       DAZOXR010000421         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZOXR010000421 DAZOXR010000000
VERSION     DAZOXR010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.430"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHUNN_004656"
     CDS             complement(<1..643)
                     /locus_tag="ACHUNN_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0578796.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUNN_004657"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUNN_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0578797.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAZOXR010000422         1074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZOXR010000422 DAZOXR010000000
VERSION     DAZOXR010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.431"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            92..>1074
                     /gene="gspA"
                     /locus_tag="ACHUNN_004658"
     CDS             92..>1074
                     /gene="gspA"
                     /locus_tag="ACHUNN_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0578798.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRTIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          285 a          234 c          274 g          281 t
ORIGIN      
        1 atttatttat attagtaata tgtatttaat taataaatac atacattatt tttatgatta
       61 aggataatca ataattaaaa caggaagttc tatgtctacg agaagagaag ttattctttc
      121 ctggttgcgt gagaaacgtc agacctggcg tctgtgctat ttgttgggtg aggctggaag
      181 tggaaaaacc tggctggcgc agcaactgca aaaagataaa catcgccgtg tgattacttt
      241 aagcctcgtt gtttcctggc aaggtaaggc cgcatggatc gttaccgacg ataacgcggc
      301 tgaacagggc tgccgtgaca gcgcctggac gcgagatgag atggcggggc aattactgca
      361 tgcgcttcat cgaactgata gtcgctgccc tcttattatt attgaaaatg ctcacctgaa
      421 tcaccgccgc atacttgatg atctccaacg cgcgatatct cttattcctg acggtcaatt
      481 tttgctgatc ggcagacccg atcgaaaagt cgaacgtgat tttaaaaaac aggacattga
      541 acttgtctcg acaggacgtc tgacggagca cgaacttaag gcaagcatcc ttgaaggtca
      601 gaatattgac caacccgatc tccttttaac ctccagagtt ctgaaacgga tagctttatt
      661 atgtcggggc gatcgcagaa agctggcgct tgccggcgaa acaattcgct tattacagca
      721 agccgagcag accagcgttt tcactgcaaa acaatggcga acgatttacc gcatattggg
      781 tgataaccgc ccccggaaaa tgcagcttgc cgtggtaatg agtggaacca ttatcgctat
      841 tacctgtggt tggctactgc tgtcctcttt cacagctccg ttccccgttc ctgcgtggct
      901 ggtacccgta acgccagtag ttaaacaaga tatgactaag gatattgctc atgtggtgat
      961 gcgcgatagt gaggcgctaa gcgtgttata cggcgtatgg gggtacgagg tcccagctga
     1021 ttcggcctgg tgcgatcagg cagtaagagc ggggctggca tgtaaaagtg ggaa
//
LOCUS       DAZOXR010000423         1073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZOXR010000423 DAZOXR010000000
VERSION     DAZOXR010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.432"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..93)
                     /gene="yqcG"
                     /locus_tag="ACHUNN_004659"
     CDS             complement(<1..93)
                     /gene="yqcG"
                     /locus_tag="ACHUNN_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR0578799.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            232..903
                     /gene="queE"
                     /locus_tag="ACHUNN_004660"
     CDS             232..903
                     /gene="queE"
                     /locus_tag="ACHUNN_004660"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0578800.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          226 c          245 g          306 t
ORIGIN      
        1 gttatcatta aaaactaata cagaaaaaat aaatataatt ttggtgaatg ttttgacatg
       61 attaaatcca ttttctttat tttcttctga cataatacgt tactcccttt aattattaac
      121 gatatgtttc gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg
      181 ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac
      241 ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc
      301 atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc
      361 tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag
      421 agtgataagt ggggggcggc gagcagtgaa gacttgttgg ctgtcattag tcgccaggga
      481 tacaccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctacca
      541 ctgactgatc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggtactcat
      601 gaggtacgct gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc
      661 ggctatgacg tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg
      721 cgcgtacgcg atattgaagc actggatgaa ctattggcaa cgctgaccga tgataaaccg
      781 cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa
      841 acctgcattg cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc
      901 tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta
      961 tattgtttgt cgcctctgaa aactcctcca ttttacccat ccagggctaa tcattagcat
     1021 tctctacaaa ttctgtggca ttaatttttc gctggagtga aaattattgc ggt
//
LOCUS       DAZOXR010000424         1046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZOXR010000424 DAZOXR010000000
VERSION     DAZOXR010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.433"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHUNN_004661"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHUNN_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHUNN_004662"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHUNN_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHUNN_004663"
     CDS             728..985
                     /locus_tag="ACHUNN_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR0578801.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          179 c          198 g          312 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccct
//
LOCUS       DAZOXR010000425         1041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZOXR010000425 DAZOXR010000000
VERSION     DAZOXR010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.434"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(91..1041)
                     /locus_tag="ACHUNN_004664"
                     /pseudo
     CDS             complement(91..1041)
                     /locus_tag="ACHUNN_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          223 a          254 c          209 g          355 t
ORIGIN      
        1 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
       61 ggttgtctgg agattcaggg ggccagtcta ctgtgtttct gttagccatt gtccggcggc
      121 tttttgctgg gtaatgcttg cctgcacctc tctggctgat ctttgccaat atttagaagt
      181 taagaggagg tcgtccgcta aatcttccag acgactaccg atctgtgcgt cggtatagcc
      241 ctttttcacc attccgttga cagcttttga aattaaacca gcagagttat tggcaaagtc
      301 tttcaaatcc tttttagtta agaatccttc attcattaaa gtgttaatac cagaatcacc
      361 gcccttccat atgcctttcc ctgttttctt atcaactaca tattcagcct cgtcatagct
      421 tttggtagcg cgttccttta catctttacc gatggcatct agtatttttc tgcgtcccgg
      481 atcaccctgg aggctatcga tcccgttgtc atacgcccaa cgctcaagat tattaagtaa
      541 gttgtaatca actccaccaa gccttgcacg ttggattagc tccccttctg tttcagcatt
      601 agcaaagctg ttagccataa attcagaggt tttactaaca gccatcacgc cagcagcagc
      661 cgtagcgcct ccaaataaca gagcgcccat cgtggcggaa ttccctgatc ctcccttaac
      721 gggggcaaat tgagcttttc tgttgccatt aatcttacgt tgttgctgtt gtaggcgttt
      781 catctgacta ttcatacgcg ccaggctgat agccccttgc tcatattgtt ttgctatctc
      841 tctagcctgt gcaatagctt tgtattgttc cgctacagag agacggtgca ttgcactgat
      901 agagcttgat acatcaagga gttttaattc tgctttctct ttacgtgctg ctatttgttg
      961 ctgttgcctt gcctgtgctc ttgctgcggc agcttgtgct ctttgattct gcttttctgt
     1021 tttagctttc tgttgagcaa t
//
LOCUS       DAZOXR010000426         1040 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZOXR010000426 DAZOXR010000000
VERSION     DAZOXR010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.435"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUNN_004665"
     CDS             complement(<1..906)
                     /locus_tag="ACHUNN_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR0578802.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          293 a          237 c          209 g          301 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg
//
LOCUS       DAZOXR010000427         1028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZOXR010000427 DAZOXR010000000
VERSION     DAZOXR010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.436"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            9..944
                     /locus_tag="ACHUNN_004666"
                     /pseudo
     CDS             9..944
                     /locus_tag="ACHUNN_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            946..>1028
                     /locus_tag="ACHUNN_004667"
     CDS             946..>1028
                     /locus_tag="ACHUNN_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR0578803.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNI"
BASE COUNT          333 a          188 c          265 g          242 t
ORIGIN      
        1 acacgtaggg tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
       61 tcacgcagag tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc
      121 ggacaacaga atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg
      181 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
      241 ctgacgggct ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga
      301 acccgcatca gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg
      361 gcctgtatta taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg
      421 atccgattgg gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata
      481 gcatggatcc attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat
      541 ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa
      601 tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata
      661 gtgtagactt gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca
      721 ctcacggttt ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg
      781 atagtctaaa tttttcaagc aaagatttaa cgaattctta tatgaatgga atgggaaaaa
      841 aagaatacag tagttacttg ggaacaccaa ataacaccta tctaaaatat aatcccaaag
      901 ctaaagggaa tggagttaca attatcaggc aagggagtaa ttaaaatgaa ggcaatacaa
      961 tgttgttatt tccttgcttt agtctccagt catttaatgg ctggagttat gggtgatgat
     1021 aatatatt
//
LOCUS       DAZOXR010000428         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZOXR010000428 DAZOXR010000000
VERSION     DAZOXR010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.437"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUNN_004668"
     CDS             complement(77..865)
                     /locus_tag="ACHUNN_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0578804.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUNN_004669"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUNN_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0578805.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZOXR010000429         1000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZOXR010000429 DAZOXR010000000
VERSION     DAZOXR010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.438"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>1000
                     /locus_tag="ACHUNN_004670"
     CDS             <1..>1000
                     /locus_tag="ACHUNN_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFR0578806.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          234 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattggt
//
LOCUS       DAZOXR010000430          982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZOXR010000430 DAZOXR010000000
VERSION     DAZOXR010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.439"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..828)
                     /gene="ipaH3"
                     /locus_tag="ACHUNN_004671"
     CDS             complement(<1..828)
                     /gene="ipaH3"
                     /locus_tag="ACHUNN_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0578807.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGPRIYFSMSDGQQNTL
                     "
BASE COUNT          278 a          158 c          249 g          297 t
ORIGIN      
        1 gagtgtattc tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg
       61 ttgggcggtt tgttgcgaca gagactccct gatccgtgag gacagaggat tgtcttcgag
      121 gatgatagtg caggtcggat caaggctaag tatattttcc ggaatgtatg tgatgcgatt
      181 ctcgcggcac cggaaaaata tctcggtttc ctctgagtga tgatttctta caggtacggc
      241 tggtaggctt tccagaagat tagtacttac atcgagcgct tccagtgatt caggtaactc
      301 agggagaaat gtcagctggt tatttcttac tgagagcact tccagcgatg taggtaattc
      361 aggaagcatg gttagctgat tgttatctgc attaatatat tccagcaatg caggcaattc
      421 aggaagcatg gttagttggt tgttatctac atcaagatgt ttcagagatg cgggtaattc
      481 aggaagtgtt gacaggtgat tgtcacaggc gtcaaggtat tccagcgatg ctggcaattc
      541 tggtaatgat attagggcat tctgagtaat ttccagaaca gtgatttgag gtggtaagtt
      601 gtcaggtagc gaggacagat ttaaacgatt cagttgaagc tcactgaact gattgatgag
      661 acattcttta agtagggaga ccgcttcatt ccgattttca ccggggagcg cttgtttttc
      721 ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt tataaaaaga
      781 attttgggac aatgaaaagt tattatttat cggtaacatg atggacattc agttaaagcc
      841 gatggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt tcattccatg
      901 aaatgaactt cagcactttt ttattataaa aggacaaaca aaattggctg taaaaaaacg
      961 atactgtttc agttaactgt ga
//
LOCUS       DAZOXR010000431          967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZOXR010000431 DAZOXR010000000
VERSION     DAZOXR010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.440"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..885)
                     /locus_tag="ACHUNN_004672"
     CDS             complement(<1..885)
                     /locus_tag="ACHUNN_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HFR0578808.1"
                     /translation="MRKNNKTSVLLIIIILAMILGVVKIQVWLNDWNNDFFNALSQKE
                     TDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIRWREWLTDYYLNRWFADKNYYFTQ
                     IYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQSLSMLITFTVILWQSAGTLSFTV
                     GGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRPLNVEKQRSEATFRTNLVQHNKQT
                     ELIALSNAESLQRQELRDNFHTIKENWHRLMNRQRWLDYWQNIYSRSLSVLPYFLLLP
                     QFISGQINLGGLMKSRQAF"
BASE COUNT          294 a          202 c          206 g          265 t
ORIGIN      
        1 aaatgcctgg cgcgatttca tcagtccgcc cagattgatc tgcccgctaa taaactgcgg
       61 caataacaga aagtagggaa gaacgctaag cgagcgcgaa taaatatttt gccagtaatc
      121 aagccaccgc tgacgattca ttaaacgatg ccagttctct ttaatggtat gaaaattgtc
      181 gcgcaactcc tggcgttgta gagactcagc attgcttagc gcaatcagtt ctgtctgctt
      241 attatgctgc acaagattag ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag
      301 cggacgaata cgtttaccca ccttatgggt aaataaagtt ccaccgatca caatgagcac
      361 gacggtatag accatatacc cctggatatt ccattctgtt ccaccaacag tgaatgagag
      421 cgtacccgcg ctttgccaaa gaataacggt aaaggtgatc agcatgctaa gtgactggat
      481 gaagccaaat gaaagactga gcgttttgct gatcagtaag agaatgtctt cagcgatacg
      541 ttggtcaggg ttatctgtat ttttatgctc gccgtagatc tgcgtgaagt agtagttttt
      601 atctgcaaac cagcgattaa ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag
      661 tagtttaatt aaccatgttt tgtttacgga tattaataca aagatcccca gcagggcagg
      721 gaaccaaaga acaagctgcc aaagcttatc ggtttctttc tggcttagcg cattgaaaaa
      781 atcattattc cagtcgttta accagacctg aattttaacc acgccaagga tcattgcgag
      841 gataataatg attaacaaca ctgaagtttt attattctta cgcagccaaa agggcttcag
      901 cagacagaga tactttgcta tcaacatata aaacgtaatg ggaatggtta tcattagcga
      961 aaattga
//
LOCUS       DAZOXR010000432          965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZOXR010000432 DAZOXR010000000
VERSION     DAZOXR010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.441"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..965
                     /gene="espL4"
                     /locus_tag="ACHUNN_004673"
                     /pseudo
     CDS             1..965
                     /gene="espL4"
                     /locus_tag="ACHUNN_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT          345 a          185 c          170 g          265 t
ORIGIN      
        1 tttctctgca aatattaata atacagccca gacaaatgaa caccaaaccc tgagtgaatt
       61 gttttataaa gaactcgagg ataaattttc tggcaaggag ctggcgacgc ctctattaaa
      121 aagcttctca gagaactgta gacaaaatgg tcgtcatatc tttagcaaca aggattttgt
      181 cattaaattt tccacgtccg tcttacaagc tgataagaaa gaaattacga taattaataa
      241 aacgaaaaca cgacactcac tcaaaccatt gctccaatat ttgaagaata cctaatggaa
      301 attttacctc aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga
      361 aaagaaaaag aattcccaag aattaaactt aatggtcaat gctattttcc ggggcgaccc
      421 caaaaccgta tagtatgccg acacattgct gcacaatata ttaatgatat ttatcagaat
      481 gttgattaca aaccccatca agatgattat tcttcagctg aaaaatttct cactcacttc
      541 aacaaaaaat gcaaaaacca gactttggcg ttgatttcca gccgtcctga ggggcgttgc
      601 gttgctgcct gcggtgattt cgggctagtt atgaaagcat attttgacaa gatggaatca
      661 aatggcatca gtgttatggc agccatatta ctggtggata accatgcttt gacggtccgg
      721 ctaagaataa agaacacaac tgaaggatgt acccattacg tggtttcggt ttatgatcct
      781 aatgtaacta acgacaaaat aagaatcatg agcgaaagca aagaggatat taaacactat
      841 tctctgatgg attttatgaa tgtagattat agcctcctga aatggtcaaa tgatcatgtt
      901 attaaccaat ctgttgcaat aattccagca cttccgaaag aacagctatt gatattaaaa
      961 ggatc
//
LOCUS       DAZOXR010000433          962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZOXR010000433 DAZOXR010000000
VERSION     DAZOXR010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.442"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..400
                     /locus_tag="ACHUNN_004674"
     CDS             <1..400
                     /locus_tag="ACHUNN_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0578809.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            574..>962
                     /locus_tag="ACHUNN_004675"
     CDS             574..>962
                     /locus_tag="ACHUNN_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0578810.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          328 a          177 c          190 g          267 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgttcag agagcaccta agaccatctc tattagcggt
      781 gttgtagtgg ttgattcgac gggttcagtt ctattaacca gacaggggca acttgtagaa
      841 acctttattt ctacggttga acaatggcga gaccagaagc aaatcatcac tattgtttgt
      901 agagatggaa tagctatcac tgatagtatc attactcagt ttaaagcgag taaagagcat
      961 ac
//
LOCUS       DAZOXR010000434          959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZOXR010000434 DAZOXR010000000
VERSION     DAZOXR010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.443"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>959
                     /locus_tag="ACHUNN_004676"
     CDS             <1..>959
                     /locus_tag="ACHUNN_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HFR0578811.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          272 a          173 c          266 g          248 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggta
//
LOCUS       DAZOXR010000435          958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZOXR010000435 DAZOXR010000000
VERSION     DAZOXR010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.444"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            6..958
                     /locus_tag="ACHUNN_004677"
                     /pseudo
     CDS             6..958
                     /locus_tag="ACHUNN_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAZOXR010000436          942 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZOXR010000436 DAZOXR010000000
VERSION     DAZOXR010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.445"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            192..>942
                     /locus_tag="ACHUNN_004678"
     CDS             192..>942
                     /locus_tag="ACHUNN_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR0578812.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQ"
BASE COUNT          207 a          291 c          246 g          198 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      781 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      841 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      901 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gc
//
LOCUS       DAZOXR010000437          934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZOXR010000437 DAZOXR010000000
VERSION     DAZOXR010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.446"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(196..>934)
                     /locus_tag="ACHUNN_004679"
     CDS             complement(196..>934)
                     /locus_tag="ACHUNN_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0578813.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          258 t
ORIGIN      
        1 tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg ttgatgactt
       61 gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat ttttaaggat
      121 tatcctgtct atgattttct acttagaagg atatggcact aactatgcat cttatagttt
      181 actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga tagccatacc
      241 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
      301 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      361 cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc ggatatacag
      421 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      481 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      541 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      601 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      661 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      721 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      781 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      841 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      901 tggtgaaaca aataaaaaag tgagagtcgg acca
//
LOCUS       DAZOXR010000438          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZOXR010000438 DAZOXR010000000
VERSION     DAZOXR010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.447"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..933
                     /locus_tag="ACHUNN_004680"
                     /pseudo
     CDS             1..933
                     /locus_tag="ACHUNN_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          296 a          189 c          186 g          262 t
ORIGIN      
        1 aattgcaatc caatccataa tcaataaaaa caataatcct tcagagttca ggattatggc
       61 tactggtcat gcccatttag atatagcgtg gatgtggcct ttaagagaag gacgcagaaa
      121 agcaataaga acatttgcca ccgcccttgc aaacattgaa aaatatcctg attacatttt
      181 tggcgctagt cagtaccaac tttttcactg gataaaaaaa gattatccct atttcttcga
      241 aaagttaaaa aaacaaatcg ccgcaggccg ctttgagtta caaggttgct tctgggtcga
      301 atgcgattta aatctggtaa gtggtgaatc gctgataagg caaatcatgc atgggacacg
      361 attcacactg cagaattttt caaaaaagat aaattatgtc tggcaaccgg atgtctttgg
      421 attccccgca acgcttccac aaattctcaa aaaatcaggc attaactata ttgcatcgca
      481 aaagctctca caaaacaaga ttaataaatt taataattac cttttccgct ggcagggcct
      541 ggatggctca gaaattctga tgcataactt tcctgaagac acttatgata gccgagcacg
      601 tgctcgctct ttagaatata ttgaacaaaa ctataatgaa aaagagatct gtccctatgc
      661 gcttatggtg tatggtgtat ggtgtcggtg atggcggcgc tggccccggt gaggaacata
      721 tagaacgatt aacacgtata cgcaatattg atggcttacc tcatgttgat ttcagccgtg
      781 ttgataagtt cttcacttac gccgacgcct ttcgcgaaag cttgcccatt atttcaggtg
      841 aactttattt cgaggctcat caagggtgct ttacatcaga gtcagcaact aaagctcata
      901 atcgaaatat ggaaaataaa ttacatgatg ctg
//
LOCUS       DAZOXR010000439          931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZOXR010000439 DAZOXR010000000
VERSION     DAZOXR010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.448"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUNN_004681"
     CDS             complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUNN_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0578814.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPS"
     gene            complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUNN_004682"
     CDS             complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUNN_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0578815.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          267 a          219 c          190 g          255 t
ORIGIN      
        1 cgaaggttct aatcgtgata ctggcggaat aacggtaaac ggcgctttgc ttttatcctc
       61 tttattaaat ggatctaccc aagcctggat gagataaggt attttttctt ctttgctaaa
      121 tacagaaata gatttatcag cattattact taagtaaacc acccgcgtac caccaatgac
      181 aacgcctgca taagttaatg ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa
      241 taacttacat gaaactgttg ttttaaagag cctgattaaa caactttttc tggacataac
      301 gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt cttatattcg
      361 acagtaaatt gtgaatcagc gttcgccgta ccagccgtta cggtcgcatt ggttgccaca
      421 tagcgtgcaa taaacttcat ttcagccttg ccttcagcat cgatagcggt ataagcagag
      481 tcgttataaa gtttaacctg cgaattatcg gactggttaa aaatacgaat accaacgccg
      541 gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc
      601 gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa acgaacggct
      661 gcttgagtgt acgttgccgg gcaattttca agtttaatac tgaacttctg tggcgacgcc
      721 agactaccta cacctgaaaa tgcattttta ctgactttac ccagtgggac aatttgatcg
      781 gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa gtgaacaaca
      841 ccatcttctg ccagtgcatg cccggccagc attagagatg ccagagtgaa tgcaccgcct
      901 ataatattac gcttcataaa aactccattg a
//
LOCUS       DAZOXR010000440          918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZOXR010000440 DAZOXR010000000
VERSION     DAZOXR010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.449"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..804
                     /gene="gadX"
                     /locus_tag="ACHUNN_004683"
     CDS             <1..804
                     /gene="gadX"
                     /locus_tag="ACHUNN_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0578816.1"
                     /translation="CLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENF
                     VLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYA
                     EMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASE
                     LLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFI
                     YVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          264 a          176 c          209 g          269 t
ORIGIN      
        1 tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg tgaatatcgc
       61 tattttaatg gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt agataagtgt
      121 gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc gatgctcaag
      181 gaggaggcat taaatcttca tgcacataaa aaaatttctt cattactcgt tcatcactgt
      241 agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc
      301 tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt ttttcttgag
      361 gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg aacacgagtt
      421 tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat cgccagcgag
      481 ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac atcatattca
      541 cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat acatggcttt
      601 tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt catttacgtc
      661 tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc gcagggattg
      721 ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg cactgaccgt
      781 tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaacc cggcataggg
      841 gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac actccctgtt
      901 ggcacgggaa actttgtg
//
LOCUS       DAZOXR010000441          881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZOXR010000441 DAZOXR010000000
VERSION     DAZOXR010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.450"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>881
                     /gene="htrE"
                     /locus_tag="ACHUNN_004684"
     CDS             <1..>881
                     /gene="htrE"
                     /locus_tag="ACHUNN_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR0578817.1"
                     /translation="QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNE
                     QRLDIDVPQAWVMKNYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFN
                     GGMNLGAWRLRASGNYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDS
                     VSIRGIRLYSDSRMLPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVID
                     DLSPSGYGSDLIVTVEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDE
                     PNLFQASYYYGLNNYLT"
BASE COUNT          233 a          194 c          201 g          253 t
ORIGIN      
        1 tgcagtttca tataaattct cccgatataa ataacgaaaa agccgttctg cttgccaggg
       61 atgaaacact cggcaattgc cttaatttga cggaaattat ccctcaggct tctgttcgtt
      121 atgacgttaa cgagcaacgt ctggatatag acgttcctca ggcatgggta atgaaaaatt
      181 accaaaacta tgttgatcca tcgttatggg aaaatggcat taatgcggcc atgttgtcat
      241 acaacctcaa cggatatcat agtgaaactc ctggtcgaag aaatgacagc atttatgctg
      301 cttttaacgg tgggatgaat ttaggtgcat ggcgactgcg tgcctcaggc aactacaact
      361 ggatgaccga ttctggcagt aattatgatt ttaagaatcg gtatatacag cgtgatatcg
      421 cctcgctgcg ttctcaactc attcttggtg agtcttatac aacgggcgaa acctttgatt
      481 ctgtcagtat ccgcggcatt cgtttataca gcgacagccg catgttgcct ccgacattag
      541 cgagttttgc gcctatcatt catggcgttg ctaataccaa cgccaaagtc actattacgc
      601 aaggtggcta taagatttat gaaacgactg tgccgccagg cgctttcgtc attgatgatc
      661 tgagtccgtc aggttacggc agcgatctta ttgttactgt cgaagaatcc gatggctcaa
      721 agcggacatt ctcgcaacct ttttcatcgg tcgttcaaat gttacgccct ggcgttggac
      781 gttgggatat tagcggcggc caggtcttaa aagatgatat tcaggatgag cctaatttat
      841 ttcaggccag ctactactac ggtctgaata actatctaac g
//
LOCUS       DAZOXR010000442          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZOXR010000442 DAZOXR010000000
VERSION     DAZOXR010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.451"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..292
                     /locus_tag="ACHUNN_004685"
     CDS             <1..292
                     /locus_tag="ACHUNN_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578818.1"
                     /translation="LFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDK
                     KYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            304..753
                     /locus_tag="ACHUNN_004686"
     CDS             304..753
                     /locus_tag="ACHUNN_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR0578819.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          293 a          105 c          223 g          256 t
ORIGIN      
        1 tttattttgt tcaactgaat taaactcgaa atcacaatcg cctgcattag gtttgggaca
       61 tgagtttact catgcgcagt attgccttct tgataaggaa aatttcatgg ctcttttaag
      121 ccgaacagat aagaaatatg agaataaaga agaggcaagg gttattacaa taattgaatc
      181 gcgtgcggcg aaaactttag gtgagtgtac cagaggagct catagtggac tgccttttta
      241 tcgggtggat ggaccgttgc agacgatgaa aataacgggt acgccagaat agtgaggttt
      301 tttatgatga aatatttgat ggttttattg tctctatttt ctggttcggt attggggatg
      361 gggcgagtga atgaattgtg tggaatagat agcgtgaaaa cgatagagat aattaatctt
      421 ccatcgtatg ttaccacgtt agttccgctt agcaaggaag gtcttaatga aatatacaga
      481 tataaagtgg tggtgaatga aattagagat ctttatgctg gtaagataat tgatttattg
      541 caaatgaagt atttcagaaa agaaaaatat aacaatataa gatggggggg tagtatcatt
      601 tcgaaaggta ataataaatg tgaaatttat tttgatgctt ttggtgaatg tggaagtgtg
      661 aatggcatta acgtgtgttt tgaaaaaaat gaaatgatag gttggattaa aaaagaaatc
      721 cctttgctgt cgcaaaaaat aggtgggctt taaatggtag ggcaatattc aaagacagta
      781 taggtaacta tttgtatggc catgtaaggt aaaagaaaag gaataaacca tgagcggaaa
      841 accggcggcg cggcagggcg acatgacccg cagggaa
//
LOCUS       DAZOXR010000443          875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZOXR010000443 DAZOXR010000000
VERSION     DAZOXR010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.452"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>875
                     /gene="cadA"
                     /locus_tag="ACHUNN_004687"
     CDS             <1..>875
                     /gene="cadA"
                     /locus_tag="ACHUNN_004687"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0578820.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKEL"
BASE COUNT          237 a          220 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgc
//
LOCUS       DAZOXR010000444          849 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZOXR010000444 DAZOXR010000000
VERSION     DAZOXR010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.453"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            21..821
                     /locus_tag="ACHUNN_004688"
                     /pseudo
     CDS             21..821
                     /locus_tag="ACHUNN_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          242 a          167 c          207 g          233 t
ORIGIN      
        1 taatcacaag ggaatacata atgaaaagaa aagttctggc aatgctggtc ccagcgttat
       61 tagttgctgg cgcagcaaat gcggctgaaa tttataataa aaatggcaat aaactggatc
      121 tgtacggaaa agttgatgct cgtcatactt tttccgataa ccctggcgat gacggcgatg
      181 aaactatcat cgagttaggc ttcaaaggtg agacccaaat tacagatcag ttaactggtt
      241 atggtcaagc actgaccaaa acaaaggcta gcgacactga aggttctgat aatacttatg
      301 tcaaacttgc ttttgcaggt ctgaagttcg gcgagatggg ctcctttgac tatggtcgta
      361 actatggtgt gatttacgac gttgaagcct ggacagatat gcttcctgta ttcggcggcg
      421 actcctatac ctggactgat aacttcatgg caggccgtgc aaatggggta gctacttacc
      481 gcaatagtga tttcttcggt ctggtggaag gtctgaactt tgcattgcag tatcaaggta
      541 acaacgaagg cagcaatgca ggagaagatc aagaaggtac taaaaatggt cacgaagacg
      601 ttcgcttcca gaacggtgat ggttttggtc tttccacttc atatgacttt gacttcggtt
      661 tgagcttagg tgccgcttac tcaaactctg accgtaccaa ttctcaagta gctcttggcg
      721 ggtatcacta caatgaatac agcaaatttg ctggtggtga tacagctgaa gcatggacat
      781 ttggtgcaaa atacgacgcc aacaatgttt atcttgcaat gggtgatgct gccaacttac
      841 tgatttagt
//
LOCUS       DAZOXR010000445          824 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZOXR010000445 DAZOXR010000000
VERSION     DAZOXR010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.454"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            259..824
                     /locus_tag="ACHUNN_004689"
                     /pseudo
     CDS             259..824
                     /locus_tag="ACHUNN_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          290 a          150 c          143 g          241 t
ORIGIN      
        1 aaaaaatata gttcatcagg attaaaacac tcaaagaaag aggtaatttt agaatgaaat
       61 tatctgtgat gattattaat acactgcaga gaaataaaaa acaatctgaa gcatgctctg
      121 aaacatccta ctctgtcact ctaatgatgg caccttgctc ttcttataaa gaagggaaca
      181 attaagaata ttaaattttc aaactttcat caacttcaca tcaagaaatt aaaaaattta
      241 aacattaatc taaataataa caacctgatc acataatcgg gtacgatttt atccgataac
      301 tataatgcta taactctggg aaatggttgg aatacaccgc tgggtgccat ctctttttac
      361 gctgcccgat cgagtagtaa gttaaataat gataccagac acgaaggtac gagctatcag
      421 gtcgcctata ataaatatct agtacaaaca gccactcgat ttagcgtggc cgcctggcgt
      481 tatgcttcgc aggattacag gacattcagc gaccatcttt acgaaaatga taaacactat
      541 catcagagtg attatgatga tttttatgat attggcagaa aaaatagcct ttctgccaat
      601 attatgcaac ctttatccaa taatctggga aatgtatcat taagtgcgct ttggcggaat
      661 tactgggggc gaagtggaaa tgctaaagat taccaattca gttactccaa taactggcaa
      721 cacattagtt atactttctc tgccagccaa tcttatgatg aaaataataa agaagaggag
      781 cgttttaatc tgtttatctc cattcctttc tactgggggg atga
//
LOCUS       DAZOXR010000446          819 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZOXR010000446 DAZOXR010000000
VERSION     DAZOXR010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.455"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            146..>819
                     /locus_tag="ACHUNN_004690"
     CDS             146..>819
                     /locus_tag="ACHUNN_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0578821.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYA"
BASE COUNT          273 a          141 c          172 g          233 t
ORIGIN      
        1 gcagggaaag atcatcggaa gtacactttt tgtactaaat aattcgcgct aaatgttcaa
       61 taattgagat attccttatt acctaaaact atttttcata gcttatatat gatcaaatac
      121 tccttgcata aataaggaga acaaaatgga acttaaaaaa ctgatggaac atatttctat
      181 tatccccgat tacagacaag cctggaaagt agaacataaa ttgtcagata ttctactgtt
      241 gactatttgt gccgtcattt ctggtgcaga gggttgggaa gatatagagg attttggtga
      301 aacacatctc gattttttga agcaatatgg tgattttgaa aatggtattc ctgttcacga
      361 tactattgcc agagttgtat cctgtatcag tcctgcaaaa tttcacgagt gctttattaa
      421 ctggatgcgt gactgccatt cttcagatga taaagacgtc attgcaattg atggaaaaac
      481 gctccggcac tcttatgaca agagtcgccg caagggagcg attcatgtca ttagtgcgtt
      541 ctcaacaatg cacagtctgg ttctcgggca gattaagacg gatgagaaat ccaatgagat
      601 tacagccatt cctgaacttc ttaacatgct ggatattaaa ggaaaaatca tcacaactga
      661 tgcgatggga tgccagaaag atattgcaga gaagatacaa aaacagggcg gtgattattt
      721 attcgcggta aaaggaaacc aggggcggct taataaagcc tttgaggaaa aatttccgct
      781 gaaagaatta aataatccag agcatgacag ttacgcaat
//
LOCUS       DAZOXR010000447          811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZOXR010000447 DAZOXR010000000
VERSION     DAZOXR010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.456"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..811
                     /locus_tag="ACHUNN_004691"
                     /pseudo
     CDS             1..811
                     /locus_tag="ACHUNN_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          224 a          199 c          217 g          171 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat t
//
LOCUS       DAZOXR010000448          800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZOXR010000448 DAZOXR010000000
VERSION     DAZOXR010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.457"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            91..>800
                     /locus_tag="ACHUNN_004692"
     CDS             91..>800
                     /locus_tag="ACHUNN_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR0578822.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPTGKESTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQE"
BASE COUNT          256 a          167 c          179 g          198 t
ORIGIN      
        1 tcatttatta tgttcactct cttatctata tatttataag gcaattaaat gaaaaggaat
       61 ttattatctt ccgctattat agtcgccatc atggccctcg gtctgacggg ttgtgatgat
      121 aaaaaagccg aaacagaaac gcccccgcct gccaatagtc aacctgccgc accagctcct
      181 gaagctcccg ttgcaaaagc agaagctaaa cctgaaacac ctgcgcaacc ggtggtcgat
      241 gaacaagcgg ttttcgacga aaaaatggat gtctatatca agtgctacaa caagttacag
      301 atccctgtac agaacagtct ggcgcgttat gctgactggc tgaaagattt taaacagggg
      361 cctaccggta aagagagtac tgtttatggc atctacggca ttagtgaatc caacctcgct
      421 gagtgtgaaa aaggcgtaaa aagtgctgtg gcgttaacgc ctgcgctgca acctattgat
      481 ggcgtggcgg tgagttatat tgatgctgcc gtggcgttgg gtaataccat taacgaaatg
      541 gataaatatt acacccagga aaattataaa gacgatgcgt ttgcgaaagg gaaaacgctg
      601 caccagacat tcttaaaaaa tctggaatcc tttgaacctg tagcagaatc ttatcatgcg
      661 gcgattcagg aaattaatga taagcgccag cttgcggaac tgaaaaatat tgaagaaaga
      721 gaagggaaaa cattccacta ctactctctg gcagtcatga tttcagcgaa acaaattaat
      781 aacctgattt cgcaagagaa
//
LOCUS       DAZOXR010000449          798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZOXR010000449 DAZOXR010000000
VERSION     DAZOXR010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.458"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>798)
                     /locus_tag="ACHUNN_004693"
     CDS             complement(<1..>798)
                     /locus_tag="ACHUNN_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0578823.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNV"
BASE COUNT          151 a          263 c          177 g          207 t
ORIGIN      
        1 acattggttc cttcaatata ctctatattt atagtttctt cagagcttga taaaaaatct
       61 atagtttctt tcatttgtga atcttgtttt aaatactcaa cagcctgatt aaaatcactt
      121 ggatcaccgt taacatggaa ttttaaaccc aaagaatcta taaaccgaac agggtttaat
      181 ggatatttat acagattcca ttcacctctc agccctatcg gatcctgggt gatatacctt
      241 ccctgcaacg ggtcatagta ccggttgcgg ttgtagtaca gccccgactc ctcatcatac
      301 tgctgccccg gcagacggag tgactgctgg aggtgctgcg cgctggtttc gcccagcagg
      361 tttccccatt cgtcatactc cccctgccac gccgtttccc cttccgggct gatgagcgcc
      421 agcggcagtc cccggtggtc gcagtggtaa agatgaagtt tcctctccgg gatgtattcc
      481 gcctccagct gcgcggccat ctgctccgcc gtcagcccgc actgcgcaag ccatgcctca
      541 ctctccgcac tcaccgctcc cgcgcgcagt tcccgctcca gcctccccag catcaccgac
      601 agctccgccg gtagcgtcac acccgtgtcc tcctgcaaca cctccgccag gctccggtgc
      661 cgcgccttcg cctgttcgcc attctctgtt tcgattctca ggagcggcgt gaagctcccc
      721 ggctggtata ccgtctggat acgcgtggtg ccggtctgta tcgtcgtcag cctgtcaccg
      781 tcccacccgt accatgtc
//
LOCUS       DAZOXR010000450          795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZOXR010000450 DAZOXR010000000
VERSION     DAZOXR010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.459"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHUNN_004694"
     CDS             complement(<1..741)
                     /locus_tag="ACHUNN_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR0578824.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          161 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DAZOXR010000451          795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZOXR010000451 DAZOXR010000000
VERSION     DAZOXR010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.460"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..795
                     /gene="espX1"
                     /locus_tag="ACHUNN_004695"
                     /pseudo
     CDS             1..795
                     /gene="espX1"
                     /locus_tag="ACHUNN_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          307 a          136 c          112 g          240 t
ORIGIN      
        1 atctccagtc cttcatcatt tttttcaata tactcatctg agagatgcat atttttatag
       61 atgtaaactc atagaacata cagttcaatt ttatttttta aacacaaagg tatcacatta
      121 cttagattgg atgtttttga tgacagaaca agcgaatgtc tttctgaaga gattaagata
      181 taccaagaat gccatgaaaa attcgttaat tttttaaagg caaacttcaa ccaagaaata
      241 tacccacagc tatatactcc tgaaatattt tatgaggcct gcagaaatct ccagtcattt
      301 tatgatcatc aggaaacgtc acaaaacgca aaatacagtg caatagacaa gaaaaaaagt
      361 catcttaacc aggaaatcaa aaagcttatt aaaaaaaata ttttccctga gctatacaat
      421 gagcaatgca ataaaatccc ctcctcatcg atagatgata accagggaat aatgtggcag
      481 aactttaaaa ctagcaattc ggcatattca aaactatgtg gaaaattatc tttatttaaa
      541 agcgtgccca gtcggttaat agaaaaatca gcttactgct caactgaaaa catggccact
      601 aataaattta atgtcgtctt ttcttactgc ggagataata taaaggaatt tatactttta
      661 ttaccctata ataaatcact ggagatgtat gaacttaacg atcaaaagat ccagtatttg
      721 actgctctta atattaatat acataaaatg ttgctatcaa atataacaat agaaaagagc
      781 aacctttcat atggg
//
LOCUS       DAZOXR010000452          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZOXR010000452 DAZOXR010000000
VERSION     DAZOXR010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.461"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHUNN_004696"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHUNN_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZOXR010000453          784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZOXR010000453 DAZOXR010000000
VERSION     DAZOXR010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.462"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUNN_004697"
     CDS             complement(<1..228)
                     /locus_tag="ACHUNN_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR0578825.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHUNN_004698"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHUNN_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(586..783)
                     /locus_tag="ACHUNN_004699"
     CDS             complement(586..783)
                     /locus_tag="ACHUNN_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578826.1"
                     /translation="MRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAW
                     ILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          251 a          142 c          155 g          236 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tatt
//
LOCUS       DAZOXR010000454          783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZOXR010000454 DAZOXR010000000
VERSION     DAZOXR010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.463"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(19..378)
                     /gene="traA"
                     /locus_tag="ACHUNN_004700"
     CDS             complement(19..378)
                     /gene="traA"
                     /locus_tag="ACHUNN_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR0578827.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(412..639)
                     /gene="traY"
                     /locus_tag="ACHUNN_004701"
     CDS             complement(412..639)
                     /gene="traY"
                     /locus_tag="ACHUNN_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR0578828.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
BASE COUNT          252 a          193 c          128 g          210 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaggccaac gacgctcata cccacagcaa taaatacaga
       61 gatgatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc
      121 gaccagcacc tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgta tcgttacctt tagccatcag gtcgccttta ccagcagcca tagctaactc
      241 agggaacaac atcatcagaa cagcaaccgg gatcactgca cgaaccaggc gaagcatatt
      301 cagacgagtg aatttagaaa aaaacgattt attttcaacg ggcgcagaag caccctgaac
      361 acttaaaaca gtattcatat gattcctctg attaatattg acagatgaaa actaaagttc
      421 tttaaatgtt gactcgttct cttcgatcac ctccctgaat atttcttcat tataaaaatc
      481 tggataacgt tgcaaatgat cctgcaacct caccaatgct tcgtttgttt tcgttcttcc
      541 actacgctct ctggctccaa gtaataattt attcgtttcc tcatcaaggg taagtaaaac
      601 ttttctactt ggcgcagatc ttaaagctcc tttaatcacc ctccctccca taaataaaag
      661 agatttagtc aaaatattca cataagacgc acacaaaaat aaacaacaaa atagtaaatt
      721 acgttaacaa aaaccggcac ttttagtgcc gggaatgctt acatttcttt acagtgaagg
      781 ctg
//
LOCUS       DAZOXR010000455          783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZOXR010000455 DAZOXR010000000
VERSION     DAZOXR010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.464"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            17..508
                     /locus_tag="ACHUNN_004702"
     CDS             17..508
                     /locus_tag="ACHUNN_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0578829.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          296 a          120 c          125 g          242 t
ORIGIN      
        1 atcacagagg aatattatga gcacagaaac aattgaaata ttcaataata gtgatgaatg
       61 ggcaaatcaa ctaaaacacg cattatcgaa aggagaaaat ctggcattac tacatggttt
      121 aactcctgat atccttgata gaatatatgc atatgcattc gactaccatg aaaagggtaa
      181 tataacggac gcagaaattt attataaatt tttgtgcatt tatgcgttcg aaaatcatga
      241 gtatctaaaa gattttgcat cagtatgtca gccaaaaaag aaatatcaac aagcatatga
      301 cctttacaaa ctaagttaca attactcccc gtatgatgac tattcagtta tttatcgtat
      361 gggtcaatgt cagattgggg ctaaaaatat cgataacgca atgcaatgtt tctatcacat
      421 tattaacaat tgtgaggatg atagtgttaa gagtaaagcg caggcatata ttgaactctt
      481 aaacgataat tcagaagata atggctaagc attacaatca tttggttaaa tttgctggtt
      541 atagtgcagt taactactat aaccagcacc tcttatgttt tcttaaatca gcatcttaaa
      601 acagttaaga gcatactata aatcattatt tttcaatgcg ttagagaaca atctacttta
      661 taaaaattga ataaggctat ttcagattac atcactgaat tcctaagata aattagtgaa
      721 cacaaccata gatatgtcga atgtattttc ctgtagcatt aatgacacat aaatatgaat
      781 agc
//
LOCUS       DAZOXR010000456          777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZOXR010000456 DAZOXR010000000
VERSION     DAZOXR010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.465"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            19..735
                     /locus_tag="ACHUNN_004703"
     CDS             19..735
                     /locus_tag="ACHUNN_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0578830.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          252 a          137 c          168 g          220 t
ORIGIN      
        1 ttcgaggaga tcattccagt gggacgtaaa tgggccaata ttgttgctaa aaaaacggct
       61 aaagacggtg caacgtctaa aatttatgca aaattcggtg tagaaatcta tgctgctgct
      121 aaacaaggtg aacccgatcc agaattaaac acatctttaa aattcgttat tgaacgtgca
      181 aagcaggcac aagttccaaa gcacgttatt gataaagcaa ttgataaagc caaaggcggc
      241 ggagatgaaa cgttcgtgca gggacgttat gaaggctttg gtcctaatgg ctcaatgatt
      301 atcgccgaga cattgacttc aaatgttaac cgtacgattg ctaacgttcg cacaattttc
      361 aataaaaaag gcggcaatat cggagcggca ggttctgtca gctatatgtt tgacaatgct
      421 ggtgtgattg tatttaaagg atcagaccct gaccatattt ttgaaatttt acttgaagct
      481 gaagttgatg ttcgtgatgt gactgaagaa gaaggtaaca ttgttattta taccgaacct
      541 actgaccttc ataaaggaat cgcggctcta aaagcagctg gaatcactga gttctcaaca
      601 acagaattag aaatgattgc tcaatctgaa gttgagcttt ccccagaaga tttagaaatc
      661 tttgaagggc ttgttgatgc ccttgaagat gacgacgatg tacaaaaagt ttatcataac
      721 gtcgcaaatc tctaattatc ttttaaagaa atctgtcttt acggcagatt tctttaa
//
LOCUS       DAZOXR010000457          776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZOXR010000457 DAZOXR010000000
VERSION     DAZOXR010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.466"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..344
                     /locus_tag="ACHUNN_004704"
     CDS             <1..344
                     /locus_tag="ACHUNN_004704"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0578831.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>776)
                     /locus_tag="ACHUNN_004705"
     CDS             complement(406..>776)
                     /locus_tag="ACHUNN_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR0578832.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          176 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaa
//
LOCUS       DAZOXR010000458          766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZOXR010000458 DAZOXR010000000
VERSION     DAZOXR010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.467"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..717
                     /locus_tag="ACHUNN_004706"
     CDS             <1..717
                     /locus_tag="ACHUNN_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0578833.1"
                     /translation="LPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPL
                     TEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYL
                     LTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTI
                     TDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHL
                     FAHGEYIKGITSLIKYKLKK"
BASE COUNT          240 a          147 c          148 g          231 t
ORIGIN      
        1 ctgccgacgt cacagttttg gtcgtacgcg acatacttca gggtattgtc ctttgaatat
       61 ctgagtgaaa gtatttccac actgctgtat ctggatgccg atgtcgtttg taaaggaagt
      121 ctgaagcctc tcacagaaat tatatttaaa gatgagtttg ctgcggtcat tcctgacaat
      181 gttagtactc aggcggcatg tgcaaaacgc ctcaacattc ccgagatgaa tggacgttat
      241 ttcaatgcag gcgttatcta tgtcaatatt aaaaaatggc atgaagcaaa tttgacaccg
      301 tatttactca cgcttttacg aggggaaact aaatatggct ctcttaaata tttagatcag
      361 gatgcgttga atatcgcatt taatatgaat aatatctacc tcgcgaagga ttttgatact
      421 atttataccc tgaaaaacga acttcatgat cgtagtcatc gaaagtatca gcaaaccatt
      481 accgataaaa cagtattgat tcactataca gggataacta aaccatggca tagctgggct
      541 ggttatccgt ctgcatcata ctttaatatc gcgcgtgaac aatctccctg gaagaaatat
      601 cctcttaaag aggcgcggac tgttgcagaa atgcagaaac aatataagca tctgtttgcc
      661 catggtgagt atattaaagg cataacttca ttaattaagt acaagcttaa gaaataaatt
      721 actcttatga ttacaagtat acgctatcgc ggcttctcat tttatt
//
LOCUS       DAZOXR010000459          755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZOXR010000459 DAZOXR010000000
VERSION     DAZOXR010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.468"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>755
                     /locus_tag="ACHUNN_004707"
     CDS             <1..>755
                     /locus_tag="ACHUNN_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0578834.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYI
                     SSADLTAEKFATAIRNHWHVENNLHWRLDVVMN"
BASE COUNT          243 a          142 c          178 g          192 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac
       61 gagtgcttta ttaactggat gcgtgactgc cattctacag atgataaaga cgtcattgca
      121 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      241 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa
      301 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      361 ggaggtgatt atttatttgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      421 aaaaaatttc cgctgacaga attaaataat ccagcgcatg acagttccgc aatgagtgaa
      481 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      541 attgatttca cgtttgaatg gaaagggctg aaaaaattat gcgtggcagt ctcctttcgc
      601 tcaataatag cagagcaaaa gaaagagccc gaaatgatgg tcagatatta tatcagttct
      661 gctgatttaa ccgctgagaa gtttgcgaca gcgattcgaa atcactggca cgtggagaat
      721 aatctgcact ggcgtctgga cgtggtaatg aatga
//
LOCUS       DAZOXR010000460          755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZOXR010000460 DAZOXR010000000
VERSION     DAZOXR010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.469"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            89..>755
                     /gene="istA"
                     /locus_tag="ACHUNN_004708"
     CDS             89..>755
                     /gene="istA"
                     /locus_tag="ACHUNN_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR0578835.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVI"
BASE COUNT          193 a          198 c          184 g          180 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catca
//
LOCUS       DAZOXR010000461          751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZOXR010000461 DAZOXR010000000
VERSION     DAZOXR010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.470"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>751)
                     /locus_tag="ACHUNN_004709"
     CDS             complement(<1..>751)
                     /locus_tag="ACHUNN_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0578836.1"
                     /translation="ENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYK
                     QCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSA
                     YIHKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYIL
                     TLLNSMRNMDGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLD
                     VCHYDSETKVLTSHHGIIRNEENECYCLGALQ"
BASE COUNT          245 a          145 c          111 g          250 t
ORIGIN      
        1 ctgcaaagca ccgagacaat aacattcatt ttcttcattt cttattattc cgtgatgact
       61 ggttaaaact ttcgtttctg agtcataatg acaaacatcg agcgttttaa ttaagttata
      121 cgtatcaccg gcagagagtt tataattttc tagtcgtgca gtatagttac cattaaaatt
      181 gatttcatga tttccagcca gaacgatgac atttttatta atacggctat ctttattacc
      241 atccatattt cgcatagaat ttagcaaagt aaggatatat ttatcaccaa aaatagtact
      301 aaatctatcg cctgtatgat ccccaaggaa gatacaaggg gttatcgcat cgtcctttaa
      361 aaaaacatgt tgatgcaact cattcaatat catgtctctc gtatttaata aattgtagag
      421 caatttatta tttttatgta tgtatgcaga tgaagccatg ttttcgtgtt ttaataattc
      481 acataataaa ctccaaccct tttccctgat gcctatatgc cctgaaacga gcaatgcata
      541 aagtacagcg cctactcttc catctgtatc gccaaaataa gccgtgttgg cattttgtgg
      601 gttaatcaca ccacattgtt tataaaagtc tattttagaa aacacagatt taacatagct
      661 atcaaactta tccttaggaa acgtctcttt agagacatcc caacgccagt cagctttaaa
      721 attaccgcta agaagagtaa agaaattttc a
//
LOCUS       DAZOXR010000462          749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZOXR010000462 DAZOXR010000000
VERSION     DAZOXR010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.471"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            584..>749
                     /locus_tag="ACHUNN_004710"
     CDS             584..>749
                     /locus_tag="ACHUNN_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0578837.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVI"
BASE COUNT          212 a          135 c          164 g          238 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcg
//
LOCUS       DAZOXR010000463          745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZOXR010000463 DAZOXR010000000
VERSION     DAZOXR010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.472"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..723
                     /locus_tag="ACHUNN_004711"
     CDS             <1..723
                     /locus_tag="ACHUNN_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0578838.1"
                     /translation="SNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQH
                     AVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGD
                     ISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHD
                     RWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVI
                     MVTHQPGVWNLADDICDISTVL"
BASE COUNT          214 a          163 c          164 g          204 t
ORIGIN      
        1 tcgaacaatt taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt
       61 atcgatcgct tgtatgagtt ccatcaactc actgaacagc gccctacgaa taagcctaaa
      121 aattgccaac atgcggtaca agtggctaat gcgagtattc gtacgcctga taataagatc
      181 atattagaga acttgaactt tcatgtttcg ccaggcaaat ggctattact gaaaggctac
      241 tctggtgcgg gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa
      301 ggtgatattt cttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaagc
      361 ggcttactga aagagattat ttgtaaagca cttcccctgc ccgtagacga taaatcgttg
      421 agcgaagtac tgcatcaggt tggtctgggg aaattggctg cgcgtattca tgaccacgat
      481 cgctggggag atattctttc cagcggcgaa aaacaacgta tcgccctggc acgattaatt
      541 ttacgacgtc cgaaatggat atttcttgac gaaactacct ctcatcttga ggaacaagag
      601 gctatacgct tactgcgttt agtgcgtgaa aaactaccca caagcggcgt cattatggtt
      661 acacatcaac ccggtgtctg gaacctggct gatgatattt gtgacattag cacggtttta
      721 taacaacaat aaaagccctg ctgta
//
LOCUS       DAZOXR010000464          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZOXR010000464 DAZOXR010000000
VERSION     DAZOXR010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.473"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>731)
                     /locus_tag="ACHUNN_004712"
     CDS             complement(<1..>731)
                     /locus_tag="ACHUNN_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0578839.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAZOXR010000465          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZOXR010000465 DAZOXR010000000
VERSION     DAZOXR010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.474"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUNN_004713"
     CDS             <1..239
                     /locus_tag="ACHUNN_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0578840.1"
                     /translation="ADRNRSHWSTSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            344..535
                     /locus_tag="ACHUNN_004714"
     CDS             344..535
                     /locus_tag="ACHUNN_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0578841.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="ACHUNN_004715"
     CDS             complement(536..>731)
                     /locus_tag="ACHUNN_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0578842.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAZOXR010000466          730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZOXR010000466 DAZOXR010000000
VERSION     DAZOXR010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.475"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(162..>730)
                     /locus_tag="ACHUNN_004716"
     CDS             complement(162..>730)
                     /locus_tag="ACHUNN_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0578843.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          180 c          158 g          167 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga
//
LOCUS       DAZOXR010000467          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZOXR010000467 DAZOXR010000000
VERSION     DAZOXR010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.476"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHUNN_004717"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHUNN_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR0578844.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZOXR010000468          711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZOXR010000468 DAZOXR010000000
VERSION     DAZOXR010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.477"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..710
                     /locus_tag="ACHUNN_004718"
     rRNA            <1..710
                     /locus_tag="ACHUNN_004718"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          161 a          156 c          238 g          156 t
ORIGIN      
        1 tgtttgatgt tctaacgtgg acccgtgatc cgggttgcgg acagtgtctg gtgggtagtt
       61 tgactggggc ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg
      121 tcggacatca ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc
      181 gagcaggtgc gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc
      241 aacggataaa aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg
      301 gcggtgtttg gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg
      361 gtatggctgt tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt
      421 tcggtcccta tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg
      481 accggagtgg acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct
      541 aaatgcggaa gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt
      601 ctccctgacc ctttaagggt cctgaaggaa cgttgaagac gacgacgttg ataggccggg
      661 tgtgtaagcg cagcgatgcg ttgagctaac cggtactaat gaaccgtgag g
//
LOCUS       DAZOXR010000469          710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZOXR010000469 DAZOXR010000000
VERSION     DAZOXR010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.478"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZOXR010000470          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZOXR010000470 DAZOXR010000000
VERSION     DAZOXR010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.479"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHUNN_004719"
     CDS             <1..>695
                     /locus_tag="ACHUNN_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0578845.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZOXR010000471          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZOXR010000471 DAZOXR010000000
VERSION     DAZOXR010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.480"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            132..>690
                     /locus_tag="ACHUNN_004720"
     CDS             132..>690
                     /locus_tag="ACHUNN_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0578846.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRL"
BASE COUNT          193 a          157 c          192 g          148 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga
//
LOCUS       DAZOXR010000472          686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZOXR010000472 DAZOXR010000000
VERSION     DAZOXR010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.481"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(1..686)
                     /locus_tag="ACHUNN_004721"
                     /pseudo
     CDS             complement(1..686)
                     /locus_tag="ACHUNN_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246653.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          190 a          100 c          128 g          268 t
ORIGIN      
        1 tgtgacattt ttttggggtt aagcgtctcg tattgaaata cttcgaactg atattcagac
       61 atttctgccc atgtttgaat tttattgtat cgagagagtg tcgtaaaggc tttaacttgc
      121 ttataacatt gtttttggaa ttcttgctga tattttaata tgtcattgta tgaaagattg
      181 gttatcctgg cctctaaaaa tttaattatt gtcgaagcaa tttccgcttt tgctcgtaaa
      241 aagttgtcat taatggttaa taatgttgaa aataaacctt cgatttgttt gtcaagatat
      301 tgtccatctt tatataattt gggggtaggg tgttctttat gtaaaaaata gtgtcaccta
      361 aatttgcgtc agtgaaattt atattaagta gatcttcgaa ataaaacctt gacaaggtta
      421 gaacgaaccc ggacaggttc tgtcctgtca tattgaaatc aataaagttg gctccggcaa
      481 agactttcat cctgttttta ttttcactgt gctcaatata taacttcgtc attttttgat
      541 acaggctttg aatatctttt atattagttg gtaagtcagc tcgttgttca gcaataaaat
      601 tagtatctga aaacatggtt tggacactga atgttatggg tattccctct tctacgctaa
      661 ttttagttat gattgccttg tcgggt
//
LOCUS       DAZOXR010000473          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZOXR010000473 DAZOXR010000000
VERSION     DAZOXR010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.482"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..561
                     /locus_tag="ACHUNN_004722"
     CDS             <1..561
                     /locus_tag="ACHUNN_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0578847.1"
                     /translation="YRFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGK
                     DANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEV
                     PIQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILM
                     KIDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(617..>685)
                     /locus_tag="ACHUNN_004723"
     CDS             complement(617..>685)
                     /locus_tag="ACHUNN_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578848.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          154 t
ORIGIN      
        1 tatcgcttta gtcagcttga atctgcgttg aatacccaga agaactcgat tcctgcgctg
       61 gaaaaggaag tgaaagcgct tgataagcaa atggtggcgg cgcagaaagc agcggatgca
      121 tattggggaa aagatgcaaa cggtaagcaa atgacccgcg aagaagcctt taaaaaaatt
      181 caccaacagc gtgatgagtt taataaacag aacgatagcg aggcgttcgc ggttaaatat
      241 gacaaagagg tttatcaacc ggcgattgcg gcatgtcata aacagagtga agagtgttat
      301 gaagtgccga ttcagcagaa acgagatttc gatatcaacg aacaacggcg acagactttt
      361 ctgcaatcac aaaaactcag ccgtaaattg caggatgact gggtaacgct cgaaaaaggg
      421 caatatccgt taacgatgaa agtttcagaa ataaacagta aaaaagtcgc gattttgatg
      481 aaaattgacg atatcaacca agctaatgaa cgctggaaaa aagataccga acaactgcgg
      541 cgcaatgggg tgattaagta actcatgtta agtgcctttg ccggataata taaaaagtta
      601 tccggcaaat tattttttac attagcccta gtagctgtgg aatgaacaag gatatttgcg
      661 gaatgtaagt gatcatcatc aacgc
//
LOCUS       DAZOXR010000474          682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZOXR010000474 DAZOXR010000000
VERSION     DAZOXR010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.483"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHUNN_004724"
     CDS             complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHUNN_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0578849.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT          203 a          166 c          180 g          133 t
ORIGIN      
        1 gcacaataaa gccgatacag acgctaccga ggatcattgg aatgccgatt ttagagacga
       61 tatcgatccc caacgcggta gttggtgccc agacaattac cagaatcatg gtcaacgtct
      121 cacacaacat gccgccgaga atactgacgc gccagcgctg tgctttgggg attttcaggt
      181 ttatccagcc tgatatacaa cccgccagaa tgctggtgat aaagcagggg atcgccgtca
      241 cgccgccgat atcaattaaa taccggtgaa tacccgcaat cacgccggta atgatgccta
      301 cccacgggcc aaacagaatc ccgtcggaca tcaccgcgat aatacgtacg ttcaccagcg
      361 agccttctac gggaacgccg gaccaggtgc tgaacagggc gaacagcgag aaaatggcgg
      421 taacggcgag caattctttt ggggagtgcg ccgacttgtg caacagttcg cgaaacaggc
      481 ggatacggat gagaaagaac aggcagataa gcattaacgc tgcccgatcg aagaccgcca
      541 gcagcatgtt gaatatttcg tgcacgggtg actctgaata aggattaaac ctctatgata
      601 ggtaacctga aggctgatga ccagcaggcc gtttttgagg aaaaaccata aatttaaaag
      661 gggattgaaa gataaatggt gt
//
LOCUS       DAZOXR010000475          677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZOXR010000475 DAZOXR010000000
VERSION     DAZOXR010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.484"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>677
                     /locus_tag="ACHUNN_004725"
     CDS             <1..>677
                     /locus_tag="ACHUNN_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0578850.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQ"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAZOXR010000476          677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZOXR010000476 DAZOXR010000000
VERSION     DAZOXR010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.485"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>677
                     /locus_tag="ACHUNN_004726"
     CDS             <1..>677
                     /locus_tag="ACHUNN_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR0578851.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     IMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSA"
BASE COUNT          166 a          163 c          209 g          139 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggatcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgcgc
//
LOCUS       DAZOXR010000477          666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZOXR010000477 DAZOXR010000000
VERSION     DAZOXR010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.486"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          210 a          159 c          131 g          166 t
ORIGIN      
        1 gacagacctg tgctatcgga aagaaaaaag gccatcagca aaactctgat ggcctgaatc
       61 acctttacca atattgtatg aaaaaacacg cacgactaat tgacaataat tttcatttcc
      121 attaaaaaag atcgcgtagc attcttaatt catgaggaac tttacccgcc agcaatctga
      181 gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg tccttttttc tacggtcctc
      241 ctccccggag gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa
      301 aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatttc
      361 cagctcacac ataccgagcg ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt
      421 tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac aacaccagaa
      481 tgatgaatca tcagtcccct gccagaggtt cactacgtaa cctgaccgac aatgcaccat
      541 tctggtgctg aaaaaagagc actggaactg caacaaaaca acatcacaga acaggagatg
      601 gtaaggagtc agacattccc acacaatatt gtgtcagtgc aaataacaac cttcgtctca
      661 aatctc
//
LOCUS       DAZOXR010000478          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZOXR010000478 DAZOXR010000000
VERSION     DAZOXR010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.487"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..311
                     /gene="tnpA"
                     /locus_tag="ACHUNN_004727"
                     /pseudo
     CDS             1..311
                     /gene="tnpA"
                     /locus_tag="ACHUNN_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          210 a          148 c          127 g          177 t
ORIGIN      
        1 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
       61 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      121 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg tgactgcgac
      181 gagatcgacc agatattgca gtcaggtatt actacaaagg cgttttgtgg agtgttggct
      241 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      301 agcaaacacc tggttaggtg gaaaaccacg ccttatatcc ctgccctgaa ggacggggtt
      361 ttacggcgca ccggataagc ctggagatta tctacaccag catggacatc attgtactat
      421 gccccgtaca tgccccatca catcctcaac atcaaagtat tagagccttg caaaccatta
      481 tgcgtctcag tttgtctcat ctatcttacg caaaaaaaaa tagaactaat atcaagcggc
      541 gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca
      601 tgcaaataat ttccatggtt atttctaggc tatttaaatt aaataaataa attatgagtc
      661 aa
//
LOCUS       DAZOXR010000479          657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZOXR010000479 DAZOXR010000000
VERSION     DAZOXR010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.488"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>657)
                     /locus_tag="ACHUNN_004728"
     CDS             complement(<1..>657)
                     /locus_tag="ACHUNN_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0578852.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     S"
BASE COUNT          105 a          248 c          202 g          102 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgcacctgca tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaggaag gagctgagag agggcaggga ctgtcagggg aagataacga
      301 agcggtgtgc ggtttacggg cctcttccgc ccgctgcgcc tgtgtggtca gcaccaggtg
      361 aaaacggcgg cccgcgccgt ccatcacccc cgtcaccgcg cccgccacgt caccctccgc
      421 tgcccggtga aaggccagcg tccgcccgaa gccgtccacc acgcccgtca gcacccggta
      481 tgcgggcggc tccggcggca gcacctcgtc cgcccccggc acccgctccg gccagctgag
      541 tatccaccac ggcccctgaa ggctgtttgt cgccaggtac acatgcggac tcaggcgaac
      601 atcttccggc agcacctgcc agagcgcgct tagcggctgc gaactgtgct gcgccgc
//
LOCUS       DAZOXR010000480          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZOXR010000480 DAZOXR010000000
VERSION     DAZOXR010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.489"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUNN_004729"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHUNN_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0578853.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZOXR010000481          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZOXR010000481 DAZOXR010000000
VERSION     DAZOXR010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.490"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHUNN_004730"
     CDS             complement(<1..396)
                     /locus_tag="ACHUNN_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0578854.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          194 a          151 c          102 g          193 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt
//
LOCUS       DAZOXR010000482          639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZOXR010000482 DAZOXR010000000
VERSION     DAZOXR010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.491"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHUNN_004731"
                     /pseudo
     CDS             complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHUNN_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(415..>639)
                     /locus_tag="ACHUNN_004732"
     CDS             complement(415..>639)
                     /locus_tag="ACHUNN_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0578855.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          164 a          173 c          137 g          165 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaatccggc aacagacata cctactgcaa taaatacaga
       61 gagtatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgactgcacc
      121 gaccagaact tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgtt tcctgacctt tagccatcag gtccgtgcct ttaccagcag ccatagctaa
      241 ctcagggaac aacatcatca gaacagcaac cgggatcact gcacgaacca ggcgaagcat
      301 attcagacga gtgaatttag aaaaaaacga tttctttttg acgggcgcag aagtaccctg
      361 aacacttaac gctgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
      421 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggaggagt
      481 atggcccagc ctttccagca atcgtcgatt gttataccag tccacccacg tgagtgtggc
      541 cagctccact tctgtccggt ttttccagct cttacggtgt attacctccg ctttgtaaag
      601 gcccttgatg atctctgcca tcgcgttgtc atacgagtc
//
LOCUS       DAZOXR010000483          628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZOXR010000483 DAZOXR010000000
VERSION     DAZOXR010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.492"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUNN_004733"
     CDS             complement(<1..304)
                     /locus_tag="ACHUNN_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0578856.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          193 a          144 c          143 g          148 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataaca
//
LOCUS       DAZOXR010000484          624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZOXR010000484 DAZOXR010000000
VERSION     DAZOXR010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.493"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>624)
                     /locus_tag="ACHUNN_004734"
     CDS             complement(<1..>624)
                     /locus_tag="ACHUNN_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578857.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          109 a          227 c          191 g           97 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgaacctgcg tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaaacct gagaagagga aacagaacgg ggactggccg gggaagataa
      301 ggaagaggcg cgctgtttac gggacgcttc cgcccgctgc gcctgcgtgc tcagcgccat
      361 gtgaaaacgg cggcccgcgc cgtccgtcac ccccgtcacc gcgcccgcca cgtcaccctc
      421 cgccgcccgg tggaaagtca gtgagcgccc gaagccatcc accacgaccg tcagtacccg
      481 gtatgcgggc ggctccggcc agctgagtat ccaccacggc ccctgaaggc tgtttgtcgc
      541 caggtacaca tgcggactca ggcgaacatc ttccggcagc acctgccaga gcgcgcttag
      601 cggctgcgaa ctgtgctgcg ccgc
//
LOCUS       DAZOXR010000485          623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZOXR010000485 DAZOXR010000000
VERSION     DAZOXR010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.494"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>623)
                     /locus_tag="ACHUNN_004735"
     CDS             complement(<1..>623)
                     /locus_tag="ACHUNN_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR0578858.1"
                     /translation="IIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSGAYQKLT
                     IGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASS
                     YGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATRNQLLK
                     SLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTL"
BASE COUNT          184 a          141 c          120 g          178 t
ORIGIN      
        1 tcgagagtac ttatgatttc gtcagtacgc tcacttcttt tggcaataaa ttgttctggg
       61 gtgatacctt taggatgtat ggattcaaac ttctgaccat cattaatcca gtcgaaaagc
      121 acctcaatat cataataaag ggatttcagt agttgattac gtgtcgcgat atgaacatca
      181 gtaataatcc ctccattagt aacatagcca aataaatcgt tgcaggcagc atcaatccaa
      241 aactcaacct cgtcattttc actaacgaga gaaaggtcaa ccacccattt acctggttta
      301 tctggcggta cgccataaga tgatgcttta ttcgtgatgc cgcagatggg tgaaccattc
      361 ttatccacaa taagaccttc gccaccgata tcaacgagag cgacgagttt gtgtctaaat
      421 ttatctgctg gacgtttacc ataaactcgc atccaggcac agtcaaacag ttgaccccag
      481 tctgagccaa tggtgagttt ctggtaagcg ccgctacggc gataaacaaa aggctgcggt
      541 tcttttgtta tccatgcttc aatcgacatt tcagagacaa cagtataaac acgctgacct
      601 attttattcg acagtttgat aat
//
LOCUS       DAZOXR010000486          621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZOXR010000486 DAZOXR010000000
VERSION     DAZOXR010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.495"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            271..>621
                     /locus_tag="ACHUNN_004736"
     CDS             271..>621
                     /locus_tag="ACHUNN_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578859.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQI"
BASE COUNT          240 a          120 c           86 g          175 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat t
//
LOCUS       DAZOXR010000487          620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZOXR010000487 DAZOXR010000000
VERSION     DAZOXR010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.496"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(269..>620)
                     /locus_tag="ACHUNN_004737"
     CDS             complement(269..>620)
                     /locus_tag="ACHUNN_004737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578860.1"
                     /translation="YIPSLMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYL
                     LNHYTESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQN
                     PFNIINVICAKEHI"
BASE COUNT          194 a          128 c          110 g          188 t
ORIGIN      
        1 gcctggcata gcaagccctt ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct
       61 gtttagaaat gccaaattgt tgatctaaca aataaacctg tataaacaga acaccattta
      121 atctattccc ctgtgtaacc caccacatca cacaataaaa acaaaaaact ttaattatat
      181 ttcatagcac acaaaagtca atagaagccc tttcgtgctc ctacgtaaaa aatccatcac
      241 ttttccactg atacatcaat tcaaggcgtt atatgtgttc cttagcacat ataacattta
      301 ttatgttaaa tggattttgg cctatatttt gacctgcacc cgcataaaca ctggtttcat
      361 cgcttacagc ccttgtgaga cgtggtgctg tttctagcat ctgatccgac ataaaaccga
      421 tcattctcgt gtttagtgaa agtttagtga gagacattac agaaaggctt tctgtgtaat
      481 gatttaatag gtaaaaacga tgttgattac gcaagaaaat tccttgctga tatttgggga
      541 ttctggattt tccagatcgg ggggcgctga aaatcgcgtt aagcacctct cgcaatctag
      601 ccataaggga agggatatag
//
LOCUS       DAZOXR010000488          604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZOXR010000488 DAZOXR010000000
VERSION     DAZOXR010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.497"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..95)
                     /locus_tag="ACHUNN_004738"
     CDS             complement(<1..95)
                     /locus_tag="ACHUNN_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578861.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTG"
     gene            complement(105..>604)
                     /locus_tag="ACHUNN_004739"
     CDS             complement(105..>604)
                     /locus_tag="ACHUNN_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578862.1"
                     /translation="PWLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIWFS
                     VAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVILV
                     GGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMYVLN
                     TPPVI"
BASE COUNT          206 a          131 c          103 g          164 t
ORIGIN      
        1 ccggtaaaaa acaaagaagc attgaatgca gggaaaaata atatggccat aaaaaacatc
       61 gaaagaaact cttttaattt aacatgtaaa cgcatggtta atcctcatat cacgggtgga
      121 gtgttaagaa catacataaa tggagtcatg ttttcccttt tccatttatc aagttcctgt
      181 tgccgtttta gtccatctct aattgcatat tttaattttt ctgataaatg gcattgagca
      241 tcgatttcat ttaaaacaac tgtacacact atccctgtca gtaaaattgc tcctccgaca
      301 agaattactg gaaagccaaa gctaacacag gcaaaagaag ccaggcttcc aatagcggtt
      361 gcaacaccac cggctataac tgctttggcc acatccattg aaaaatcgcc aagaaaatta
      421 accagatctc gctctgaact cataataaat tctatggttc tccaggctac tgaaaaccag
      481 atacagaact taactccttt tattgattca gataaagctc ctcgccagcc aatagcgagt
      541 tccagaactt gaggcgctcg tgaagagaaa atatatttac cgagcttttc taaaagccag
      601 gggc
//
LOCUS       DAZOXR010000489          597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZOXR010000489 DAZOXR010000000
VERSION     DAZOXR010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.498"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUNN_004740"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUNN_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0578863.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          130 a          162 c          171 g          134 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctggggg
//
LOCUS       DAZOXR010000490          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZOXR010000490 DAZOXR010000000
VERSION     DAZOXR010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.499"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUNN_004741"
     CDS             <1..91
                     /locus_tag="ACHUNN_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578864.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUNN_004742"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUNN_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0578865.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZOXR010000491          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZOXR010000491 DAZOXR010000000
VERSION     DAZOXR010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.500"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAZOXR010000492          574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZOXR010000492 DAZOXR010000000
VERSION     DAZOXR010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.501"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..516)
                     /locus_tag="ACHUNN_004743"
     CDS             complement(<1..516)
                     /locus_tag="ACHUNN_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0578866.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          160 a          137 c          115 g          162 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttac
//
LOCUS       DAZOXR010000493          567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZOXR010000493 DAZOXR010000000
VERSION     DAZOXR010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.502"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            21..>567
                     /locus_tag="ACHUNN_004744"
     CDS             21..>567
                     /locus_tag="ACHUNN_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0578867.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          217 a          117 c           74 g          159 t
ORIGIN      
        1 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
       61 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      121 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      181 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
      241 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      301 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acatctatca
      361 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
      421 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
      481 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
      541 cagttactta ttttagtgcg gcacata
//
LOCUS       DAZOXR010000494          566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZOXR010000494 DAZOXR010000000
VERSION     DAZOXR010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.503"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..225
                     /locus_tag="ACHUNN_004745"
     CDS             <1..225
                     /locus_tag="ACHUNN_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0578868.1"
                     /translation="DSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            267..467
                     /locus_tag="ACHUNN_004746"
                     /pseudo
     CDS             267..467
                     /locus_tag="ACHUNN_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          174 a          128 c          142 g          122 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccatactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcaacc ttcacgataa aaaacttttc attaaaggta
      301 aagcggatca atatccggca cttcattctc tgatatctcc gacccgcatt aatctgaaag
      361 aaatagaaat tcactggcgt gaagtgctac gtctggcgac ctcaataaaa cagggaacag
      421 tgacagcatc cctgatgctg agaaaacttg ccagttaccc caaacaaaag taaggcagta
      481 tgactttccg tgagacagat gctggccaac gtgggatatt taacgcgggt taacatcgtt
      541 gtcagagccg catgggtgag ctgttg
//
LOCUS       DAZOXR010000495          564 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZOXR010000495 DAZOXR010000000
VERSION     DAZOXR010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.504"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..531)
                     /gene="ytfI"
                     /locus_tag="ACHUNN_004747"
     CDS             complement(<1..531)
                     /gene="ytfI"
                     /locus_tag="ACHUNN_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR0578869.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVS"
BASE COUNT          168 a          107 c           86 g          203 t
ORIGIN      
        1 gcttactata tcctccaata aggcataacc taaagacacg tcaccaatcg cctggtgaat
       61 ataaccttta atccttttcg gtatgtcaga ctgttttttc tgcagaatat cacaggcata
      121 caatcttctg ttttgttcta acggaaactc gcggtttttg aaatccatga atgagttata
      181 ctcatttatt gttatgggaa tttcttcgta ttcaaatcca ctacctcgca atgtcattgt
      241 atattcggca ccattatctt taaggctgaa actgataagg aacttatcca tggcgttaac
      301 agttccattt tttgatgaaa gtacctgata actattttta tcaatctgag ttgcgcaacg
      361 aaagaactcc ctcaaagtta acaggctatc ataattcgaa atccaatctt ttttcttaaa
      421 tgcatgctgg atattgttaa aaaaattgtt tgaatgtgtt tttgtatacg atgactttac
      481 aaataactcc accgcaccaa taaaattatt gtgtctgatc ctgggtaaca tatgacaacc
      541 ttattttatt tggttatttt atgt
//
LOCUS       DAZOXR010000496          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZOXR010000496 DAZOXR010000000
VERSION     DAZOXR010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.505"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..428
                     /locus_tag="ACHUNN_004748"
     CDS             <1..428
                     /locus_tag="ACHUNN_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0578870.1"
                     /translation="QLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          163 a          124 c          156 g          116 t
ORIGIN      
        1 aacagcttac cagcgtggcg aggcagaatg aaacctgtaa gcggttgctg gatattcctg
       61 gcgttggacc acttattgcg acggcagcgg tggccaccat gggggaagca tcagcgttta
      121 aatcggggcg agagttcgcc gcatatgttg gtctggttcc aaaacaaact ggctccggag
      181 ggaaagtacg tctgctgggg ataagcaaac gtggtgacac ttatctcagg acattattta
      241 tccacggtgc aagagcggtg gcattagtag ctaaagagcc tggcccgtgg ataaccgaac
      301 tgaaaaaacg tcgtccagcc agtgtggcaa tcgtcgccat ggcaaacaag ctggcacgaa
      361 cagtatgggc gataaccgcc catgaccgta agtatgacag gaaccacgtc agtatcagac
      421 catattaatc gctgatacca ttaaacaatg aactcttaac aaaagggtga atgctgaaag
      481 gttgctatgg cggccagagt gatgacaaag acaggtaaga ccgtgactca ctaaacctga
      541 acagtatttt gggcttgaa
//
LOCUS       DAZOXR010000497          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZOXR010000497 DAZOXR010000000
VERSION     DAZOXR010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.507"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUNN_004749"
     CDS             <1..>557
                     /locus_tag="ACHUNN_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0578871.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZOXR010000498          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZOXR010000498 DAZOXR010000000
VERSION     DAZOXR010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.508"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUNN_004750"
     CDS             <1..>557
                     /locus_tag="ACHUNN_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0578872.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZOXR010000499          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZOXR010000499 DAZOXR010000000
VERSION     DAZOXR010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.509"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUNN_004751"
     CDS             <1..>557
                     /locus_tag="ACHUNN_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR0578873.1"
                     /translation="IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEY
                     ARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHR
                     RHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          142 a          169 c          148 g           98 t
ORIGIN      
        1 tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa gcagaaaacg gcgtcctcat
       61 cgtcgagcga tggcttcttg cccgcatccg taatgaaacc ttccacacgc tcagggcgct
      121 gaatgcacgc cttcgggagt tgctgacgga catgaataac cgaccaatga agggatacgg
      181 taatcaaacc cgcgcagaac gcttccgcat gctggatgca ccagcactct ctccgctgcc
      241 tctggagcct tatgaataca cggaatacaa agccgtgaag gttggccctg actaccacgt
      301 tgaatatgcc cgtcactggt actccgttcc acacgaactg gtcggtcagc gtctgtcact
      361 gaaggtgggg cagtcggtgg tacagctctg gcacaagggg cagtgtgtgg cgcaacatcc
      421 gcgcagcaca catgaatata aacacacaac caatccgctt cacatgccag aacgccaccg
      481 gcggcatggg acatggacac cggaacgcct tatcgagcag ggaaaccgta caggtccgtc
      541 cacaggccgg gtggttg
//
LOCUS       DAZOXR010000500          552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZOXR010000500 DAZOXR010000000
VERSION     DAZOXR010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.510"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>552
                     /gene="feaR"
                     /locus_tag="ACHUNN_004752"
     CDS             <1..>552
                     /gene="feaR"
                     /locus_tag="ACHUNN_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR0578874.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          141 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca at
//
LOCUS       DAZOXR010000501          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZOXR010000501 DAZOXR010000000
VERSION     DAZOXR010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.511"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUNN_004753"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUNN_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZOXR010000502          544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZOXR010000502 DAZOXR010000000
VERSION     DAZOXR010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.512"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          160 a          105 c          103 g          176 t
ORIGIN      
        1 atctcttctg atgtttaatt gatttgaatg ttcgtaagct atatcactta ctcaatccat
       61 tttacccaga gtcataaaat agagtgtctg ttgatctcat tttcatattc ctttcagact
      121 gaagtgcgtt ttcatcaccc gatgagaaag aactgaagtg atgtttgcca tagattgcga
      181 gatgccctgt ctcattgact atcgcggtgc acctttatac ctgttatacc agatcaaaaa
      241 tcacgcaagc catacaacaa aacgagattt gcaattcgtg tcacaaaata tgtcgatctt
      301 tttctaagag gaagatgcca tgtgaagcca gacgaacact tgaggtggtc ttcaaaaact
      361 aaaggatttc ttttaactgt ttgttttata aaaaatttat ttagggtcta aacaagggaa
      421 gctttgcaag ctaactcagt gagcgtggtg aaaacaagtg tttacccgcc atcaggctga
      481 tcataattct caccatgaaa tatgtttcct gatttgtggc ttgtaactgg tcgcttctga
      541 agtc
//
LOCUS       DAZOXR010000503          530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZOXR010000503 DAZOXR010000000
VERSION     DAZOXR010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.513"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..503
                     /locus_tag="ACHUNN_004754"
     CDS             <1..503
                     /locus_tag="ACHUNN_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0578875.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSY
                     REVNRD"
BASE COUNT          193 a           94 c           94 g          149 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt
//
LOCUS       DAZOXR010000504          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZOXR010000504 DAZOXR010000000
VERSION     DAZOXR010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.514"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>520
                     /locus_tag="ACHUNN_004755"
     CDS             <1..>520
                     /locus_tag="ACHUNN_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0578876.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAZOXR010000505          517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZOXR010000505 DAZOXR010000000
VERSION     DAZOXR010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.515"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>517)
                     /locus_tag="ACHUNN_004756"
     rRNA            complement(<1..>517)
                     /locus_tag="ACHUNN_004756"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a          170 c          110 g          140 t
ORIGIN      
        1 gcgtgccttc tcccgaagtt acggcaccat tttgcctagt tccttcaccc gagttctctc
       61 aagcgccttg gtattctcta cctgaccacc tgtgtcggtt tggggtacga tttgatgtta
      121 cctgatgctt agaggctttt cctggaagca gggcatttgt tgcttcagca ccgtagtgcc
      181 tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc ctacacgctt
      241 aaaccgggac aaccgtcgcc cggccaacat agccttctcc gtcccccctt cgcagtaaca
      301 ccaagtacag gaatattaac ctgtttccca tcgactacgc ctttcggcct cgccttaggg
      361 gtcgactcac cctgccccga ttaacgttgg acaggaaccc ttggtcttcc ggcgagcggg
      421 cttttcaccc gctttatcgt tacttatgtc agcattcgca cttctgatac ctccagcatg
      481 cctcacagca caccttcgca ggcttacaga acgctcc
//
LOCUS       DAZOXR010000506          516 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZOXR010000506 DAZOXR010000000
VERSION     DAZOXR010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.516"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            71..146
                     /locus_tag="ACHUNN_004757"
     tRNA            71..146
                     /locus_tag="ACHUNN_004757"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
     gene            340..>516
                     /locus_tag="ACHUNN_004758"
     rRNA            340..>516
                     /locus_tag="ACHUNN_004758"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          144 a          110 c          132 g          130 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcgaaag aaacatcttc gggttgtgag gttaagcgac taagcgtaca
      361 cggtggatgc cctggcagtc agaggcgatg aaggacgtgc taatctgcga taagcgtcgg
      421 taaggtgata tgaaccgtta taaccggcga tttccgaatg gggaaaccca gtgtgtttcg
      481 acacactatc attaactgaa tccataggtt aatgag
//
LOCUS       DAZOXR010000507          512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZOXR010000507 DAZOXR010000000
VERSION     DAZOXR010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.517"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>512
                     /locus_tag="ACHUNN_004759"
     CDS             <1..>512
                     /locus_tag="ACHUNN_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0578877.1"
                     /translation="INLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNVRVIS
                     KSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFVAM
                     EPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKLEVNA
                     AQKLVNAGNQR"
BASE COUNT          155 a           99 c          105 g          153 t
ORIGIN      
        1 attaatctct tatcacggct tacgccgctt ttattcgtgt tcgctccttt tttggcacag
       61 tcaaatatga ctgtttatcc tatggctgtt tccattaata gtcagggtga aggcaacgtt
      121 cgcgtaatat ctaaaagtaa tgaagttcag tacatcaagg cgacagtatt ccgtatcgat
      181 aatccatcga cgcctcagga aaatgaagtt gagattaaat ccggtgatgc aaatcatctg
      241 gttgttatgc cacctaaatt cgctttacca gccggtagta gcaaaactgt gcgctttgtt
      301 gcaatggaac cagagcaaaa agaaaaaaat tatcgcgtta aatttgaagc ggttcccagt
      361 attgatgacg ttgccacaga taaaaaagat ctctctatgc agttaacagt taacttaatt
      421 tgggggattg ttgttagtgt tccacctcag caacctattg ctaagttaga agtaaatgct
      481 gcccaaaaat tagttaatgc agggaatcaa cg
//
LOCUS       DAZOXR010000508          509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZOXR010000508 DAZOXR010000000
VERSION     DAZOXR010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.518"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHUNN_004760"
     CDS             complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHUNN_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR0578878.1"
                     /translation="LDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPA
                     GSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQLNYVV
                     SVYDPNDTNVAVRGTHRAARGFLSLDKFISSAPDAQTWADRYVRNCAIAILPLLPEGV
                     PVAILAGITT"
BASE COUNT          149 a          115 c          117 g          128 t
ORIGIN      
        1 gtcgtgatac ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca
       61 attgcacagt tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg
      121 aacttatcaa gcgagagaaa gccccgtgct gccctgtggg tgcctctgac ggcaacgttg
      181 gtatcgttcg ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt
      241 attcgtagcc ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg
      301 acattgtttg ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc
      361 gcgataacgc gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg
      421 actaagggga catgctgctg tatggattca acggaggagt agtgtttaat caggttgact
      481 ttgccttttc catttctcaa tgaatcaag
//
LOCUS       DAZOXR010000509          507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZOXR010000509 DAZOXR010000000
VERSION     DAZOXR010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.519"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHUNN_004761"
     CDS             complement(<1..188)
                     /locus_tag="ACHUNN_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0578879.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="ACHUNN_004762"
                     /pseudo
     CDS             242..445
                     /locus_tag="ACHUNN_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a          136 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAZOXR010000510          504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZOXR010000510 DAZOXR010000000
VERSION     DAZOXR010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.520"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHUNN_004763"
     CDS             <1..194
                     /locus_tag="ACHUNN_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0578880.1"
                     /translation="LKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKIV
                     VKYNNWITKDNGEFELAVH"
     gene            224..>504
                     /locus_tag="ACHUNN_004764"
     CDS             224..>504
                     /locus_tag="ACHUNN_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0578881.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          107 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcct
//
LOCUS       DAZOXR010000511          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZOXR010000511 DAZOXR010000000
VERSION     DAZOXR010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.521"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUNN_004765"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUNN_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0578882.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZOXR010000512          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZOXR010000512 DAZOXR010000000
VERSION     DAZOXR010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.522"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHUNN_004766"
     rRNA            <1..>476
                     /locus_tag="ACHUNN_004766"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZOXR010000513          474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZOXR010000513 DAZOXR010000000
VERSION     DAZOXR010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.523"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>474)
                     /locus_tag="ACHUNN_004767"
     CDS             complement(<1..>474)
                     /locus_tag="ACHUNN_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0578883.1"
                     /translation="VYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGMS
                     WAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPIA
                     QELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLPFYAS"
BASE COUNT          163 a          107 c           98 g          106 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag acaa
//
LOCUS       DAZOXR010000514          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZOXR010000514 DAZOXR010000000
VERSION     DAZOXR010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.524"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHUNN_004768"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHUNN_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZOXR010000515          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZOXR010000515 DAZOXR010000000
VERSION     DAZOXR010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.525"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..286
                     /gene="traL"
                     /locus_tag="ACHUNN_004769"
     CDS             <1..286
                     /gene="traL"
                     /locus_tag="ACHUNN_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR0578884.1"
                     /translation="KYRFPETLTNQSRWFGLPLDELIPAAICVGWGITTSKYLFGIGA
                     AVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
     gene            308..>471
                     /locus_tag="ACHUNN_004770"
     CDS             308..>471
                     /locus_tag="ACHUNN_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFR0578885.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGF"
BASE COUNT          113 a          106 c          115 g          137 t
ORIGIN      
        1 gaaatatcgt ttcccggaaa cactgaccaa ccagagccgc tggtttggcc tgccactgga
       61 tgaactgatc cccgcagcaa tctgtgttgg ctggggtatc acaacatcga aatatctgtt
      121 cggtattggt gcagcggttc tggtttattt cgggattaaa aaactgaaaa aagggcgggg
      181 cagttcctgg ttacgtgacc tgatttactg gtatatgcca acagccctgc tgcgcggtat
      241 ttttcataat gttcccgatt cgtgtttccg gcagtggatt aaatagaact gataccagga
      301 ttgttatatg gaacacggtg cccgtttaag taccagtcgt gtaatggcca tcgcctttat
      361 atttatgtca gtgcttattg ttctcagcct ctctgttaac ctcattcagg gggtgaataa
      421 ctaccgtctt cagaatgagc aacgcactgc cgtgacccca atgggattta a
//
LOCUS       DAZOXR010000516          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZOXR010000516 DAZOXR010000000
VERSION     DAZOXR010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.526"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHUNN_004771"
     CDS             <1..>461
                     /locus_tag="ACHUNN_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0578886.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAZOXR010000517          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZOXR010000517 DAZOXR010000000
VERSION     DAZOXR010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.527"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHUNN_004772"
     CDS             <1..386
                     /locus_tag="ACHUNN_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0578887.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcac
//
LOCUS       DAZOXR010000518          450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZOXR010000518 DAZOXR010000000
VERSION     DAZOXR010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.528"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            42..>450
                     /locus_tag="ACHUNN_004773"
     CDS             42..>450
                     /locus_tag="ACHUNN_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0578888.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT          133 a           77 c          110 g          130 t
ORIGIN      
        1 ttagaaaata cgtttaacga taattaaaag gattctgtta aatgaaatta aaaatagttg
       61 cggtggttgt aactggtttg ttagctgcga acgtagcaca cgctgccgaa gtctataaca
      121 aggatggtaa taaactcgac ctttatggca aggttaccgc tctacgttat tttactgatg
      181 ataagcgtga cgatggtgat aaaacttatg cccgtctcgg ctttaaagga gaaacgcaaa
      241 tcaatgatca aatgattggt tttggtcact gggaatatga ttttaaaggc tataacgatg
      301 aagccaacgg ctcgcgcggc aacaagaccc gtctggccta tgctggttta aaaattagtg
      361 aatttggctc tctggactat ggtcgtaact acggtgtcgg ctacgacatt ggttcatgga
      421 ccgatatgtt gccagaattt ggtggcgata
//
LOCUS       DAZOXR010000519          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZOXR010000519 DAZOXR010000000
VERSION     DAZOXR010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.529"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>449
                     /locus_tag="ACHUNN_004774"
     CDS             <1..>449
                     /locus_tag="ACHUNN_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0578889.1"
                     /translation="QRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSE
                     TVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPE
                     SNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          111 a          108 c          145 g           85 t
ORIGIN      
        1 tcagcgatgg tgctctgacg ggttcgagtt ccgctgtgat aacggagaaa aactgcgagt
       61 cacgttcgcg ctggactgct gtgaccgtga ggcactgcac tgggcggtca ctacgggcgg
      121 cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc gcttcggcaa
      181 cgagcttccg gcgtctccag tagagtggct gacggataat ggttcatgct accgggctaa
      241 tgaaacacgg cagtttgccc ggatgttggg gcttgaaccg aagagcacgg cggtgcggag
      301 tccggagagt aacggcatag cagagagctt cgtgaaaacg ataaagcgtg actacatcag
      361 tgtcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg cgttcgagca
      421 ttataacgaa tggcatccgc atagtgcac
//
LOCUS       DAZOXR010000520          448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZOXR010000520 DAZOXR010000000
VERSION     DAZOXR010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.530"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..152
                     /locus_tag="ACHUNN_004775"
     CDS             <1..152
                     /locus_tag="ACHUNN_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0578890.1"
                     /translation="VALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGC
                     GPTAQ"
BASE COUNT          117 a          109 c           80 g          142 t
ORIGIN      
        1 aagttgcact ggtaggtgca gtactggcta cgttaactgc atgtactggt catattgaaa
       61 accgtgataa gaactgctct tacgactacc tgctgcaccc ggcaatttct atttctaaaa
      121 tcattggcgg ttgcggtcct actgcacagt aagtctctga cagaaatcgg ctaacaccgg
      181 tttaattgcc cgacgccctc tggattttcc gagggcgtat ttgtttctat gtccttcttg
      241 cccccgcccg taaaaatatt ttcactttaa attcaatttg ataactacat ccattagcca
      301 cagcgcgatc ctgtgcgaaa ttttgtgatc ttcctccaca ttacataaca tcatatgttg
      361 ttatattcat catgcattgt catgttacct tttaaatgac tgcaaactct cccctacaac
      421 gtattggaca agaaaaaggt atcgctat
//
LOCUS       DAZOXR010000521          447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZOXR010000521 DAZOXR010000000
VERSION     DAZOXR010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.531"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUNN_004776"
     CDS             <1..203
                     /locus_tag="ACHUNN_004776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578891.1"
                     /translation="IINTVDIHSSYPVHQKQKLTDAGLKLMATAKMPDALRLSGLRHL
                     YNILNLRAFVGRIRRSRSIRHE"
BASE COUNT          125 a          113 c           92 g          117 t
ORIGIN      
        1 ctataattaa taccgtagat attcattcat cttaccctgt gcaccaaaag caaaaactca
       61 ctgacgcggg tttgaaactg atggcaaccg caaaaatgcc tgatgcgcta cgcttatcag
      121 gcctacgcca tctctacaat atattgaatt tgcgtgcttt tgtaggcagg ataaggcgtt
      181 cacgcagcat ccggcatgaa taaagcgcac tcaacaatct gaaacccgcc ggaacggttt
      241 attacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agtgtcgtgg
      301 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt
      361 aaatcaggtg aaactgaccg ataagccgct ttcttttggg tatagcgtcg tggacagtca
      421 ttcatctttc tgcccctcca aaagcaa
//
LOCUS       DAZOXR010000522          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZOXR010000522 DAZOXR010000000
VERSION     DAZOXR010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.532"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>444
                     /locus_tag="ACHUNN_004777"
     rRNA            <1..>444
                     /locus_tag="ACHUNN_004777"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAZOXR010000523          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZOXR010000523 DAZOXR010000000
VERSION     DAZOXR010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.533"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHUNN_004778"
     CDS             <1..>442
                     /locus_tag="ACHUNN_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0578892.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZOXR010000524          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZOXR010000524 DAZOXR010000000
VERSION     DAZOXR010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.534"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..120)
                     /locus_tag="ACHUNN_004779"
     CDS             complement(<1..120)
                     /locus_tag="ACHUNN_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578893.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEI"
     gene            complement(376..>441)
                     /locus_tag="ACHUNN_004780"
     CDS             complement(376..>441)
                     /locus_tag="ACHUNN_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0578894.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          128 a           90 c           87 g          136 t
ORIGIN      
        1 aatttctgca aactttcttt ttaccgtcca ataatcaata tttctattgt gattgttaac
       61 cactgcgtgc attggcggga ttgaagggtg aaaaatatgt gcaattggga agcctttcat
      121 tgatgcctgc cttgtcgtca tgacaaacaa taatagatgc ttattttcct cgcagggttc
      181 tttcgcacta tcaaaaaacg ctcatttacc cggcgttaca gtaaacgaag caacattctg
      241 tggcctacga cgatcaaaag ctctgtgaaa atttcccttg ttgtctttgt ctgaccttat
      301 cgcggcgatc agcaattatt cgttttgtga tcctcaacac aaaatgctat tcgtcgccta
      361 tgaggggaga agaatttatg gaaaacgatt gcattcacaa aatgacgtcc tgtcagaaga
      421 gaaaaagttg acgttaatca a
//
LOCUS       DAZOXR010000525          425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZOXR010000525 DAZOXR010000000
VERSION     DAZOXR010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.535"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>425)
                     /locus_tag="ACHUNN_004781"
     CDS             complement(<1..>425)
                     /locus_tag="ACHUNN_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0578895.1"
                     /translation="NVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDD
                     DDEIDPNYLLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYK
                     KVRFHIGCLFIRKQLLEENNLFFDEDLRLGEDLDFIYRL"
BASE COUNT          139 a           94 c           58 g          134 t
ORIGIN      
        1 gcagtcgata gataaaatcc agatcttctc ctagtcgtaa atcttcatca aaaaaaagat
       61 tattctcttc cagaagttgt tttcttataa acaagcaccc tatatggaat ctaacctttt
      121 tataaaaaaa gtcgaaaagt aaagatcctt cattaaactc tgtatttata ggtgagtaag
      181 taacaccatc ttttatataa tggccataaa gcccacaata aatcgcatcc cctcccgtct
      241 catgttgtct ggtatacatc ttcagcagat agttaggatc tatctcatca tcatcatcca
      301 taaaacatat aaactgtcct tgcgccttgc ggatacctgt attcctggca gacgaaacac
      361 cgccattttc tttattgata ataattaatc gagagtgggt taacccatac tctctcaaaa
      421 cgttt
//
LOCUS       DAZOXR010000526          423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZOXR010000526 DAZOXR010000000
VERSION     DAZOXR010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.536"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(136..348)
                     /gene="cspH"
                     /locus_tag="ACHUNN_004782"
     CDS             complement(136..348)
                     /gene="cspH"
                     /locus_tag="ACHUNN_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0578896.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT          129 a           82 c           90 g          122 t
ORIGIN      
        1 ataactatta ggtgcagaga tattcgctta aatggattag tttctttgtg gaaaagtaac
       61 tgatattata atgtatgatg ttgactattg cactattcca ataaaaccag tcagctttaa
      121 acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc gcaggccatt
      181 tacgcggcaa aattccacac gtaatcctgg tataagcact tctgcgtcgc ggggagtgaa
      241 tgcggaaata tggacctgaa cttctttacg accgtcggag gggataatga atcctttgcc
      301 gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac aaattcctta
      361 ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga tatttaagac
      421 cgc
//
LOCUS       DAZOXR010000527          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZOXR010000527 DAZOXR010000000
VERSION     DAZOXR010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.537"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUNN_004783"
     tRNA            41..116
                     /locus_tag="ACHUNN_004783"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZOXR010000528          419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZOXR010000528 DAZOXR010000000
VERSION     DAZOXR010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.538"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          139 a           80 c           77 g          123 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgct
//
LOCUS       DAZOXR010000529          415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZOXR010000529 DAZOXR010000000
VERSION     DAZOXR010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.539"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..230)
                     /locus_tag="ACHUNN_004784"
     CDS             complement(<1..230)
                     /locus_tag="ACHUNN_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0578897.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT          109 a           92 c           92 g          122 t
ORIGIN      
        1 aacggactgt ctctcccttg tcgcccatta ggctgatggt gtgttatatc ggtatcaacc
       61 ttatttgggt tgatatagaa agcctccgga accaccctgt aactcctccc gtgtagttca
      121 ggtgcaggat aaatgtttcc attccttgcc catcgtctca gcgttgatat tgatggtggg
      181 ttatccggat atctgagttt tccccacgtt ttgagtgtca caagattcat tgccatacct
      241 ctcacgatat gaccgccagt aaatatacag aatactggcg ggtgtggttg atttttaata
      301 atcagctatg tgaggtagcc tgagtttaac ggacactcct tcctgaaata gaatggcatc
      361 agaaggagct aataatgagc agaaaaaccc aacgttactc taaagagttc aaagc
//
LOCUS       DAZOXR010000530          414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZOXR010000530 DAZOXR010000000
VERSION     DAZOXR010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.540"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            13..>414
                     /locus_tag="ACHUNN_004785"
     CDS             13..>414
                     /locus_tag="ACHUNN_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR0578898.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIG"
BASE COUNT          136 a           73 c           71 g          134 t
ORIGIN      
        1 cgaaaattat ctatgaaaag caaactccag aaaattatat tatgtttatt tcttctatgc
       61 tgcatctata atttatggac attgcgtcct gtacagattt tgtatacata ctctgatgct
      121 ggcaattcag tctttctggt cgttgatcat ctgccatgga ctgacagcga taaaataaat
      181 tggtatctga agcatcagaa tgaaattaaa aatcaacatc ccctaccaga aggtagttgg
      241 catacctggt atgtcattga tatcggtaat ggcttcactg actataaaaa atatatcgaa
      301 gggccgtatg aagatctcta ctgtttcccg acaattaaga gcaatgataa ttgcatcgtg
      361 aaaaattact taatggttat taatgaatac ccttatcgta acactcatat tggt
//
LOCUS       DAZOXR010000531          409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZOXR010000531 DAZOXR010000000
VERSION     DAZOXR010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.541"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..394
                     /locus_tag="ACHUNN_004786"
     CDS             <1..394
                     /locus_tag="ACHUNN_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFR0578899.1"
                     /translation="LIILDRTWQHHDVIDIKFIADIQFNTDLCGDTYISRGPVLYAIE
                     LESDILIKKNLLNGKYFDSGYIPCSRADESIQFSYADMESFSYSTSPEGKQYIEGCFY
                     LNKTKIVRKLIPFGQTILRKVTFSNIES"
BASE COUNT          137 a           67 c           67 g          138 t
ORIGIN      
        1 acttattatt ttagatagaa catggcaaca tcatgatgtg attgacatta aatttatagc
       61 tgatatacag tttaatactg acttatgtgg tgacacttat atttccagag gtcccgtttt
      121 atatgcaatt gagcttgaga gcgatatttt aatcaaaaaa aacctgctga acgggaaata
      181 ttttgactca ggatacatac cctgtagtag agctgatgaa tctatccagt tctcttatgc
      241 tgatatggaa tctttttcat attcaacctc accagagggc aaacaataca ttgaaggctg
      301 tttttattta aacaagacta aaatagtcag aaaacttata ccatttggtc aaaccattct
      361 tcgcaaagta actttctcca atatagagag ttaatatttt cgagtgtta
//
LOCUS       DAZOXR010000532          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZOXR010000532 DAZOXR010000000
VERSION     DAZOXR010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.542"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUNN_004787"
     tRNA            54..130
                     /locus_tag="ACHUNN_004787"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUNN_004788"
     tRNA            173..248
                     /locus_tag="ACHUNN_004788"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZOXR010000533          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZOXR010000533 DAZOXR010000000
VERSION     DAZOXR010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.543"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(4..>406)
                     /locus_tag="ACHUNN_004789"
     CDS             complement(4..>406)
                     /locus_tag="ACHUNN_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0578900.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT           85 a          110 c          136 g           75 t
ORIGIN      
        1 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
       61 gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt cgttttccct gactcatttt
      121 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
      181 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
      241 ggatcgccga gttaccggcg caaggccagc ataagcagcg agatgtgcgg cagaggcgaa
      301 ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc ctgacaccga ctccgggcat
      361 actggtcagg accgggtaaa gagggtgagc aagaactcgc tgttct
//
LOCUS       DAZOXR010000534          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZOXR010000534 DAZOXR010000000
VERSION     DAZOXR010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.544"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHUNN_004790"
     CDS             complement(<1..356)
                     /locus_tag="ACHUNN_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0578901.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          105 a          101 c          118 g           77 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggtcaact g
//
LOCUS       DAZOXR010000535          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZOXR010000535 DAZOXR010000000
VERSION     DAZOXR010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.545"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>399
                     /locus_tag="ACHUNN_004791"
     CDS             <1..>399
                     /locus_tag="ACHUNN_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0578902.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatgac
//
LOCUS       DAZOXR010000536          398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZOXR010000536 DAZOXR010000000
VERSION     DAZOXR010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.546"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>398)
                     /locus_tag="ACHUNN_004792"
     CDS             complement(<1..>398)
                     /locus_tag="ACHUNN_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0578903.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAZOXR010000537          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZOXR010000537 DAZOXR010000000
VERSION     DAZOXR010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.547"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          126 a          104 c           67 g           98 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcata
//
LOCUS       DAZOXR010000538          394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZOXR010000538 DAZOXR010000000
VERSION     DAZOXR010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.548"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUNN_004793"
     CDS             63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUNN_004793"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0578904.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          102 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat ggat
//
LOCUS       DAZOXR010000539          392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZOXR010000539 DAZOXR010000000
VERSION     DAZOXR010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.549"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          118 a           68 c           81 g          125 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tg
//
LOCUS       DAZOXR010000540          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZOXR010000540 DAZOXR010000000
VERSION     DAZOXR010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.550"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>389)
                     /locus_tag="ACHUNN_004794"
     CDS             complement(<1..>389)
                     /locus_tag="ACHUNN_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0578905.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHRE"
BASE COUNT           71 a          134 c          112 g           72 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcc
//
LOCUS       DAZOXR010000541          387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZOXR010000541 DAZOXR010000000
VERSION     DAZOXR010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.551"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>387
                     /locus_tag="ACHUNN_004795"
     rRNA            <1..>387
                     /locus_tag="ACHUNN_004795"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          103 a           89 c          112 g           83 t
ORIGIN      
        1 cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca aacacgaaag
       61 tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg ggtcagcgca
      121 agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg gtcctaaggt
      181 agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga tggccaggct
      241 gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt acccgcggca
      301 agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga gccttgatgt
      361 gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZOXR010000542          386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZOXR010000542 DAZOXR010000000
VERSION     DAZOXR010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.552"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>386
                     /locus_tag="ACHUNN_004796"
     CDS             <1..>386
                     /locus_tag="ACHUNN_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578906.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAZOXR010000543          380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZOXR010000543 DAZOXR010000000
VERSION     DAZOXR010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.553"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>380
                     /locus_tag="ACHUNN_004797"
     CDS             <1..>380
                     /locus_tag="ACHUNN_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578907.1"
                     /translation="RVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKR
                     VVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYG
                     YNSQRQVTSVTYPDGLRSSREYDE"
BASE COUNT           85 a           87 c          155 g           53 t
ORIGIN      
        1 gtcgggtgac ggagcaggtc aacccggagg ggctggacta ccgctttgag tacggagaga
       61 gccgggtaat catcacggac agcctgaacc ggcgggaggt gctgtacacg gaaggcgagg
      121 gtggcctgaa gcgggtggtg aagaaggagc atgccgacgg gagcatcacc cgcagcgagt
      181 atgatgaggc ggggaggctg aaggcgcaga cggatgcggc gggacggcgg acggagtacc
      241 gactgcatat ggcgtctgga aagctgactt cagtgatact gccggacggc aggacggtac
      301 ggtatggcta taacagccag cggcaggtga cgtcggtgac gtacccggac gggcttcgca
      361 gcagccggga gtatgatgag
//
LOCUS       DAZOXR010000544          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZOXR010000544 DAZOXR010000000
VERSION     DAZOXR010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.554"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUNN_004798"
     CDS             <1..>374
                     /locus_tag="ACHUNN_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0578908.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          106 a           87 c          111 g           70 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaactcgg tggc
//
LOCUS       DAZOXR010000545          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZOXR010000545 DAZOXR010000000
VERSION     DAZOXR010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.555"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUNN_004799"
     CDS             complement(<1..304)
                     /locus_tag="ACHUNN_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0578909.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          113 a           76 c           80 g          105 t
ORIGIN      
        1 ttgaatcctc tctcggcacg gttgcaatta gcgagtgggt agtatcacca acagcgcgaa
       61 atccgcgtct gataatggat atgttctgat ataacgtgtt agtaggcaca cgcatacctt
      121 cttcttccca gactctggtg aaaaaatctt gttgtgcaac cacatcagga aaagcttcaa
      181 gtagaagtga taaacatcga ctggctggcg ttgtcagaac gacgttaaga tcggcgttat
      241 gtaccgatat taattttcgg tgctcaggcc agaactcgat attatcgtta ataatccagt
      301 acatacgtca ataaacacta aatcaatcga aatggagatc acatagtttg ctaagtatat
      361 tggtatttac ggca
//
LOCUS       DAZOXR010000546          369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZOXR010000546 DAZOXR010000000
VERSION     DAZOXR010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.556"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(166..>369)
                     /locus_tag="ACHUNN_004800"
     CDS             complement(166..>369)
                     /locus_tag="ACHUNN_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0578910.1"
                     /translation="NKCLIEATLKGFKSGELSFYEYRKNLATAGVFRWITNIHENKRY
                     YYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT           98 a           72 c           84 g          115 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttatt
//
LOCUS       DAZOXR010000547          368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZOXR010000547 DAZOXR010000000
VERSION     DAZOXR010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.557"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          132 a           54 c           71 g          111 t
ORIGIN      
        1 tgttaagtaa ctttgatgac cctaacaata gatcggaatt ttcttagagt caatggtcac
       61 tgtttttgct tgcaaagcaa atgttttggt ggttataagt cgtcttaata tagtcacgca
      121 tgcaacttgt tccatgcgga gattaaagat tggaatataa cgcgaagatg gaattaaatt
      181 acatttgcaa taattaattt attattactc aataattacc gatattatta ttgtaaatta
      241 atcattagaa tgcaaaaaga aatgtaacgg caaaataaga ggacgatagc tgagaaaaaa
      301 gcgtgctttc ccaatctggg gcaaatcacg tctgaatgac acctgaaaac aggtggtagc
      361 ctcaagaa
//
LOCUS       DAZOXR010000548          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZOXR010000548 DAZOXR010000000
VERSION     DAZOXR010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.558"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT          108 a           64 c           56 g          125 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atg
//
LOCUS       DAZOXR010000549          352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZOXR010000549 DAZOXR010000000
VERSION     DAZOXR010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.559"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHUNN_004801"
     CDS             <1..187
                     /locus_tag="ACHUNN_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0578911.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
BASE COUNT          105 a           81 c           92 g           74 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg aatatcagcg tg
//
LOCUS       DAZOXR010000550          347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZOXR010000550 DAZOXR010000000
VERSION     DAZOXR010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.560"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            207..>347
                     /locus_tag="ACHUNN_004802"
     CDS             207..>347
                     /locus_tag="ACHUNN_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR0578912.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSL"
BASE COUNT          100 a           63 c           63 g          121 t
ORIGIN      
        1 ttgcatttag ttacatgttt aacacttgat ttaagatttg taatggctag attgaaatca
       61 gatgtaatcc attagtttta tattttaccc atttagggct gatttattac tacacacagc
      121 agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt caggtgtgcg
      181 atcaataaaa aaagcggggt ttcatcatgt ttaatgaagt ccatagtatt catggtcata
      241 cattattgtt gatcactaaa ccttctttgc aggcgacggc tctcttgcag caccttaaac
      301 aatcgctggc aattacagga aaattacata atattcaacg ttctctg
//
LOCUS       DAZOXR010000551          345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZOXR010000551 DAZOXR010000000
VERSION     DAZOXR010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.561"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>345
                     /locus_tag="ACHUNN_004803"
     CDS             <1..>345
                     /locus_tag="ACHUNN_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0578913.1"
                     /translation="SNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMPGRWT
                     TQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFM
                     HDYDKTSSKMRKY"
BASE COUNT          135 a           64 c           53 g           93 t
ORIGIN      
        1 tcaaacacca tcttttcaaa tggagaaata aacaacctct tcattaaaaa aagcaacata
       61 tttggaacat cgttcactaa taccatgata aaaaatttgc gctgtgaaga tattatgcct
      121 ggccgatgga ccactcaact tgtcaataag cacttaggtt acagatacac aggagtattc
      181 aaaaccctgg caagtattga tgacaaaccg tcccgtttcg aaattttgat accattggta
      241 caaactttag ttcgcgataa tgtaaagcta aataatgatg tgtataaaga acttaataag
      301 tttatgcacg attacgacaa aacatcatca aaaatgagaa aatac
//
LOCUS       DAZOXR010000552          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZOXR010000552 DAZOXR010000000
VERSION     DAZOXR010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.562"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>344
                     /locus_tag="ACHUNN_004804"
     CDS             <1..>344
                     /locus_tag="ACHUNN_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR0578914.1"
                     /translation="LKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKI
                     VVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCIS
                     FTLRINGKDHDL"
BASE COUNT          101 a           64 c           81 g           98 t
ORIGIN      
        1 tattaaaaaa tacagaggag attcaacgcg ccgtgattct gaaaatggaa atgatgccat
       61 ttttagactc ggtgggactg gtacttgatg ataataaata ttatctcttt tcgcggaggg
      121 cgaatgataa aatcgttgtt tatcatcagg aacaagtaaa tggaccgctt gtcgacgagt
      181 cagggcgggt tatttttgcc gattttaacc catcgaaacg accgtggtcg gtggcttcag
      241 atgactctaa caacagctgg aatccggcat acaattgctt tgatcgtccg ggtaaaaaat
      301 gtatctcttt tacgctacgt atcaacggca aagatcacga tttg
//
LOCUS       DAZOXR010000553          339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZOXR010000553 DAZOXR010000000
VERSION     DAZOXR010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.563"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..261
                     /gene="istB"
                     /locus_tag="ACHUNN_004805"
     CDS             <1..261
                     /gene="istB"
                     /locus_tag="ACHUNN_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0578915.1"
                     /translation="LDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGL
                     MENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          108 a           74 c           93 g           64 t
ORIGIN      
        1 ctggacgatc tggggctgga acagttaagt aacgcccagt gtaatgacct tctggagatc
       61 acagaggatc gttacggaca aagcagcacg atagtggtaa gccagttccc ggtagataaa
      121 tggcatggtc tgatggagaa cccaacaaca gcagatgcca tcctggacag actggtacat
      181 aactcacaca gagtggtcct tcagggagag tccctgagga aaaatccccc gacagtggaa
      241 agcagcgaaa aaacgagtta aaaagcggta gaaaaagtta agttcccggt tgatcgaatg
      301 gatgcgaatc gctgatcgaa cttcgatgcc aatccgtga
//
LOCUS       DAZOXR010000554          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZOXR010000554 DAZOXR010000000
VERSION     DAZOXR010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.564"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>338
                     /locus_tag="ACHUNN_004806"
     CDS             <1..>338
                     /locus_tag="ACHUNN_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578916.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPE"
BASE COUNT           82 a          124 c           86 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaag
//
LOCUS       DAZOXR010000555          334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZOXR010000555 DAZOXR010000000
VERSION     DAZOXR010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.565"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            33..>334
                     /locus_tag="ACHUNN_004807"
     CDS             33..>334
                     /locus_tag="ACHUNN_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0578917.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQ"
BASE COUNT           75 a           83 c           92 g           84 t
ORIGIN      
        1 cgcggaatat gtgaacaacg gaggaaaaca tcatgcaata tgcgaaacct gtcactctga
       61 acgttgaaga gtgcgaccgt ctctcctttc taccgtatct gtttggcaac gattttctgt
      121 atgccgaggc gtatgtgtac gcgctggcgc aaaaaatgat gccggaatat cagggaggct
      181 tctggcactt catccgcctg ccggacggtg gcggttacat gatgccggat ggcgaccgtt
      241 tccacatggt gaacggtgca aactggtttg accgtaccgt gagtgctgat gccgcaggca
      301 tcattctgac ttcccttgtg attaaccgcc agtt
//
LOCUS       DAZOXR010000556          331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZOXR010000556 DAZOXR010000000
VERSION     DAZOXR010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.566"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHUNN_004808"
     CDS             complement(<1..229)
                     /locus_tag="ACHUNN_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFR0578918.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAP"
     gene            complement(226..>331)
                     /locus_tag="ACHUNN_004809"
     CDS             complement(226..>331)
                     /locus_tag="ACHUNN_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0578919.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT           83 a           97 c           83 g           68 t
ORIGIN      
        1 ccggggcaag ttccggcatc tcattacaca acagcacggg gtcaatcact cctgaaacac
       61 gggcctgtct gcagatgcct gtgtagatat cccgatacgc tcgtacagaa aaaatgtccg
      121 ccggtagtgt gatcagaata tccatcactt catgatctgc cccacgcaga aagaatgcgc
      181 caatgacagc gccttccagg tcatcattac gccagactgg tgttgtcatg cagccacacc
      241 tctgatacaa gaacggtagc tgggccagtt gaacgacaac cagttgcgcc ccccgtctgt
      301 gatcctgtcg gcaatgcggg ggctgatgaa c
//
LOCUS       DAZOXR010000557          323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZOXR010000557 DAZOXR010000000
VERSION     DAZOXR010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.567"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..175)
                     /locus_tag="ACHUNN_004810"
     CDS             complement(<1..175)
                     /locus_tag="ACHUNN_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578920.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLPD
                     DELETVMQRLDDAF"
     gene            complement(222..>323)
                     /locus_tag="ACHUNN_004811"
     CDS             complement(222..>323)
                     /locus_tag="ACHUNN_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0578921.1"
                     /translation="DAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
BASE COUNT           68 a           99 c           79 g           77 t
ORIGIN      
        1 caaaggcatc gtccagtcgc tgcatgacgg tttccagctc atcgtctggc aggttccagt
       61 cctgggctat gtgacgcaca tccccacggg tcagcaccac ggcaaaaacg cattcactgt
      121 ccggcacgtc tgtatccagc acttcgcgga taatttcttt aacagtacag tacattttct
      181 cgtctccttt acaggggcgg cattgccgcc ccgtccgtta atcagtccag agccgcgtaa
      241 atcgcgttgc attcagggtg aaattcgatg tgacgccaca actgcgcatc gcgcatccgg
      301 taaagctggg tcagtcctgc atc
//
LOCUS       DAZOXR010000558          323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZOXR010000558 DAZOXR010000000
VERSION     DAZOXR010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.568"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(222..>323)
                     /locus_tag="ACHUNN_004812"
     CDS             complement(222..>323)
                     /locus_tag="ACHUNN_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0578922.1"
                     /translation="DAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
BASE COUNT           66 a           99 c           80 g           78 t
ORIGIN      
        1 caaaggcatc gtccagtcgc tgcatgacgg tttccagctc atcgtctgtc agactccagt
       61 cctgggctat gtgacgcaca tccccacggg tcagcaccac ggcaaaaacg cattcactgt
      121 ccggcacgtc tgtgttcagc acttcgcgga taatttcttt aacagtacag tacatgttct
      181 cgtctccttt acaggggcgg cattgccgcc ccgtccgtta atcagtccag tgccgcgtaa
      241 atcgcgttgc attcggggtg aaattcgatg tgacgccaca actgcgcatc gcgcatccgg
      301 taaagctggg tcagtcctgc atc
//
LOCUS       DAZOXR010000559          312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZOXR010000559 DAZOXR010000000
VERSION     DAZOXR010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.570"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUNN_004813"
     CDS             complement(<1..242)
                     /locus_tag="ACHUNN_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0578923.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           90 a           64 c           71 g           87 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat ta
//
LOCUS       DAZOXR010000560          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZOXR010000560 DAZOXR010000000
VERSION     DAZOXR010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.571"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHUNN_004814"
     CDS             <1..>308
                     /locus_tag="ACHUNN_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0578924.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAZOXR010000561          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZOXR010000561 DAZOXR010000000
VERSION     DAZOXR010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.573"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUNN_004815"
     CDS             <1..>301
                     /locus_tag="ACHUNN_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0578925.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAZOXR010000562          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZOXR010000562 DAZOXR010000000
VERSION     DAZOXR010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.574"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUNN_004816"
     CDS             <1..>301
                     /locus_tag="ACHUNN_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578926.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZOXR010000563          298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZOXR010000563 DAZOXR010000000
VERSION     DAZOXR010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.575"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            137..>298
                     /locus_tag="ACHUNN_004817"
     CDS             137..>298
                     /locus_tag="ACHUNN_004817"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0578927.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT          105 a           64 c           54 g           75 t
ORIGIN      
        1 atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa
       61 aggttatcaa cttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt
      121 caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa
      181 taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac
      241 agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaatt
//
LOCUS       DAZOXR010000564          294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZOXR010000564 DAZOXR010000000
VERSION     DAZOXR010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.576"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..271
                     /locus_tag="ACHUNN_004818"
     CDS             <1..271
                     /locus_tag="ACHUNN_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578928.1"
                     /translation="TEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRL
                     QDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT           93 a           74 c           59 g           68 t
ORIGIN      
        1 aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac tcagtcgccc
       61 agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa atatgacctt
      121 acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc agggaaaaat
      181 gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag tacttctgtt
      241 attaaaaatg ctgcagatta atcacgcctg atctctgtcc cggtattttg aggt
//
LOCUS       DAZOXR010000565          293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZOXR010000565 DAZOXR010000000
VERSION     DAZOXR010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.577"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>293
                     /locus_tag="ACHUNN_004819"
     CDS             <1..>293
                     /locus_tag="ACHUNN_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0578929.1"
                     /translation="DRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMN
                     GGDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           81 g           78 t
ORIGIN      
        1 gatcgtgggc cgcaaagttt acgttgtctg cagttactgg aacaacattg ggtttgtgcg
       61 gtaagaggca atcatgaaca gatggcgatg gatgcgctgg catcccagca gatgtctttg
      121 tggttgatga atggcggcga ctggtttatt gcgctggcag ataatcaaca gaaacaagcg
      181 aaaacggcgc tggaaaaatg tcagcatttg ccctttattc ttgaagtaca cagtcgtacc
      241 ggcaaacatg ttattgctca tgccgattat ccagatgatg tttatgaatg gca
//
LOCUS       DAZOXR010000566          288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZOXR010000566 DAZOXR010000000
VERSION     DAZOXR010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.578"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT           80 a           65 c           63 g           80 t
ORIGIN      
        1 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca
       61 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      121 ttcataagcg tattatcagg attgtctgga aaattcccga tactttggac tattactcct
      181 gaacaattcg aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag
      241 gtaagcgtaa accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAZOXR010000567          283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZOXR010000567 DAZOXR010000000
VERSION     DAZOXR010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.580"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>283
                     /locus_tag="ACHUNN_004820"
     CDS             <1..>283
                     /locus_tag="ACHUNN_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0578930.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           87 g           69 t
ORIGIN      
        1 cgacttatca gtggaaaaac acagaacgat caggatatat gcgccctggc aaaagagtat
       61 gagatcgcca tgctgttggt gactaaaggt gcagaagggg tggtggtctg ttatcgagga
      121 caagttcacc attttgctgg aatgtctgtg aattgtgtcg atagcacggg ggcgggagat
      181 gcgttcgttg ccgggttact cacaggtctg tcctctacgg gattatctac agatgagaga
      241 gaaatgcgac gaattatcga tctcgctcaa cgttgcggag cgc
//
LOCUS       DAZOXR010000568          282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZOXR010000568 DAZOXR010000000
VERSION     DAZOXR010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.581"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..155)
                     /locus_tag="ACHUNN_004821"
     CDS             complement(<1..155)
                     /locus_tag="ACHUNN_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0578931.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIIL"
BASE COUNT           97 a           47 c           41 g           97 t
ORIGIN      
        1 aggataatac caagcttaac cggtgtaaat atacttccag gaggaagaaa tatagtagat
       61 tgataccaga tgatcttttt catatcaccc catatggctg aaaaagatat gccgcatgta
      121 ggttgaatta ccgtgtcaat tactacccac ttcatttgtt atgtcttatc ccacggtatt
      181 taatatagtt catttggatg ttcatttctt tattttgcat atgagtatat taccccttca
      241 aaaaataaat taattaaaac gattgcttat ataaaacaaa at
//
LOCUS       DAZOXR010000569          281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZOXR010000569 DAZOXR010000000
VERSION     DAZOXR010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.582"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT           82 a           55 c           58 g           86 t
ORIGIN      
        1 ttatttcgct gtatgataaa aaccacccgt tataatttat tagtgaaatt cgtttttcga
       61 gtgttagaaa tttatatcta aatagcgttg attaatgagc aaagccagac tcctgtaacg
      121 ctcacaagac tcatctacct gcgacgggta ttctggtcag tagtagatgt ttaaggcgtg
      181 gcagagacat ttcatcctta ctctacggca ttggcccgca tacaaatcgt ggttgtgaca
      241 ctcacttatc atcagtgagc gaacagagaa tagttcagtg a
//
LOCUS       DAZOXR010000570          279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZOXR010000570 DAZOXR010000000
VERSION     DAZOXR010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.583"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(50..>279)
                     /gene="trbI"
                     /locus_tag="ACHUNN_004822"
     CDS             complement(50..>279)
                     /gene="trbI"
                     /locus_tag="ACHUNN_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR0578932.1"
                     /translation="QTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHA
                     VILVSPAVVQGAPDITREIQQDIARRMRAEP"
BASE COUNT           51 a           88 c           73 g           67 t
ORIGIN      
        1 gcggccacac tctgccccca tatcagcagg gcaatcagcc cccggcatct catggttccg
       61 ctctcattcg ccgggcaata tcctgctgga tttcccgggt gatatccgga gccccctgta
      121 ccacggcagg cgacaccaga atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg
      181 cttccagtgc cgtgttaaaa cgcgctgaaa gggcttttga ctgtgcttct gacagttgtt
      241 tctgactggc gctgtcaaaa aatgcatcca cagtctgtt
//
LOCUS       DAZOXR010000571          277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZOXR010000571 DAZOXR010000000
VERSION     DAZOXR010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.584"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            36..>277
                     /locus_tag="ACHUNN_004823"
     CDS             36..>277
                     /locus_tag="ACHUNN_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0578933.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           84 a           58 c           56 g           79 t
ORIGIN      
        1 ttttaatgct atccaatcat tttaaggagt ttaaaatgga taagaagcaa gtaacggatt
       61 taaggtcgga actactcgat tcacgttttg gtgcgaagtc tatttccact atcgcagaat
      121 caaaacgttt tccgctgcac gaaatgcgcg acgatgtcgc attccagatt atcaatgacg
      181 aattatatct tgatggcaac gctcgtcaga acctggccac tttctgccag acctgggacg
      241 acgaaaatgt ccacaaattg atggatttat ccattaa
//
LOCUS       DAZOXR010000572          275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZOXR010000572 DAZOXR010000000
VERSION     DAZOXR010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.586"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT           74 a           49 c           69 g           83 t
ORIGIN      
        1 cgtgagagct gcacattatt ggcggggccg gggaaaacat cattttggga acaatgaatc
       61 aggtactctt accactgaag caagatcatc cgggcaccag gtggtggtgc agtgctttgg
      121 aaatggtcct ctcgggggga gttgccatta ttttctatgc tatttgctta ttatattcat
      181 caggtgaatt taataaattc ctaatttgaa atatccacta ttaagccagt gtttaacgac
      241 gttaagatgg caatgtgcag atcatgttat caggg
//
LOCUS       DAZOXR010000573          272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZOXR010000573 DAZOXR010000000
VERSION     DAZOXR010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.588"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>272
                     /gene="hlyE"
                     /locus_tag="ACHUNN_004824"
     CDS             <1..>272
                     /gene="hlyE"
                     /locus_tag="ACHUNN_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0578934.1"
                     /translation="NKYLDQVIPWQTFDETIKELSRFKQEYSQAASVLVGDIKTLLMD
                     SQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQKDILI"
BASE COUNT           85 a           53 c           58 g           76 t
ORIGIN      
        1 ataataaata tctcgatcag gtcatcccct ggcagacctt tgatgaaacc ataaaagagt
       61 taagtcgctt taaacaggag tattcacagg cagcctccgt tttagtcggc gatattaaaa
      121 ccttacttat ggatagccag gataagtatt ttgaagcaac ccaaacagtg tatgaatggt
      181 gtggtgttgc gacgcaattg ctcgctgcgt atattttgct atttgatgag tacaatgaga
      241 agaaagcatc cgcccagaaa gacattctca tt
//
LOCUS       DAZOXR010000574          266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZOXR010000574 DAZOXR010000000
VERSION     DAZOXR010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.589"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT           80 a           50 c           44 g           92 t
ORIGIN      
        1 tgcaatagta gcatattttg ttttccataa gcataaatct gccactctat ccatatgcag
       61 cttagtgttc ttagtgttaa ttgtaggtgc taacgtaggg tttatcagtt acagcgacct
      121 aacacaaaaa gcgacgcaaa aattagatag tttgtctaaa taattatgtt gccatgcgaa
      181 gtatgcatgg ctgcatgtct gccttccatt taaaatgggc taagacctat aaccctaaat
      241 attattcttt attatcttct ttacca
//
LOCUS       DAZOXR010000575          264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZOXR010000575 DAZOXR010000000
VERSION     DAZOXR010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.590"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>264
                     /locus_tag="ACHUNN_004825"
     CDS             <1..>264
                     /locus_tag="ACHUNN_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0578935.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSL"
BASE COUNT           71 a           77 c           67 g           49 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actg
//
LOCUS       DAZOXR010000576          262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZOXR010000576 DAZOXR010000000
VERSION     DAZOXR010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.591"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            82..>262
                     /locus_tag="ACHUNN_004826"
     CDS             82..>262
                     /locus_tag="ACHUNN_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0578936.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGK"
BASE COUNT           80 a           63 c           62 g           57 t
ORIGIN      
        1 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
       61 gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc
      121 cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata
      181 tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga
      241 caacaatggc gcgagggaaa gc
//
LOCUS       DAZOXR010000577          259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZOXR010000577 DAZOXR010000000
VERSION     DAZOXR010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.592"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            2..>259
                     /gene="istB"
                     /locus_tag="ACHUNN_004827"
     CDS             2..>259
                     /gene="istB"
                     /locus_tag="ACHUNN_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0578937.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQ"
BASE COUNT           74 a           61 c           76 g           48 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacag
//
LOCUS       DAZOXR010000578          251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZOXR010000578 DAZOXR010000000
VERSION     DAZOXR010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.593"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>251
                     /locus_tag="ACHUNN_004828"
     CDS             <1..>251
                     /locus_tag="ACHUNN_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578938.1"
                     /translation="NHQDKHSDKVNSHQHHGKVDKKYRSNTVEVNMLNDFSQIDNDLG
                     LNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYN"
BASE COUNT           98 a           39 c           45 g           69 t
ORIGIN      
        1 aaccatcaag ataagcattc agataaagtt aacagtcatc aacaccatgg caaggttgat
       61 aaaaaatatc gatcaaacac agttgaagtt aatatgctga atgacttttc gcaaatagat
      121 aacgatttag gcctgaatat catctatttc ctgcaacatg gtcactggag ggtaaatgaa
      181 catagtcttc agatggagaa aatatggttt tacaatagtg agccatctat agatattcaa
      241 gaatataata g
//
LOCUS       DAZOXR010000579          249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZOXR010000579 DAZOXR010000000
VERSION     DAZOXR010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.594"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>249)
                     /locus_tag="ACHUNN_004829"
     CDS             complement(<1..>249)
                     /locus_tag="ACHUNN_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0578939.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARK"
BASE COUNT           58 a           70 c           55 g           66 t
ORIGIN      
        1 ctttctggca tagctgtttc cggctttggt aatgctgccc tgatgtctgc ttccacccga
       61 tgaatattct gatggcgtga taccaaacca tgacatcagt tgtcgtggat gttcaaaacg
      121 ggtcagatca cccagttcag aaattaatcc aaccgcagta ataaactgaa tccctcgcat
      181 ggcttgcagc gcctctacta ccggatacag cctccattcg gtaacggcct ccttcagggc
      241 ggattccag
//
LOCUS       DAZOXR010000580          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZOXR010000580 DAZOXR010000000
VERSION     DAZOXR010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.595"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(<1..>243)
                     /locus_tag="ACHUNN_004830"
     CDS             complement(<1..>243)
                     /locus_tag="ACHUNN_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578940.1"
                     /translation="GLFILSGENKIDEECFINIVQTAVAINNIGRESNGYILVGVSDT
                     KATADRVKTLYGVTPIECNGYYINGIDHEAVIQSKN"
BASE COUNT           75 a           48 c           37 g           83 t
ORIGIN      
        1 atgttttttg attgaattac agcttcatga tcaattccat tgatataata gccattgcac
       61 tcaatagggg taacaccata taaagtctta actctgtctg ccgtagcttt ggtatcggaa
      121 acaccaacta aaatataacc attagattct ctaccgatgt tatttatggc gacagccgtt
      181 tggactatat taatgaaaca ttcttcgtct attttattct cgcctgagag aataaataat
      241 ccc
//
LOCUS       DAZOXR010000581          242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZOXR010000581 DAZOXR010000000
VERSION     DAZOXR010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.596"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHUNN_004831"
     CDS             <1..220
                     /locus_tag="ACHUNN_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0578941.1"
                     /translation="RKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEI
                     FYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT           75 a           52 c           58 g           57 t
ORIGIN      
        1 gcgtaaaggt aactgttacg acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa
       61 tgagagcctg agccactatc gttttaataa ccgggatgaa gccatctcag taatacggga
      121 atacattgag attttctaca atcgtcagcg tcgtcactct cgtctgggga atatctcccc
      181 ggcagccttc agggaaaaat atcatcagat ggctgcttaa aaaaagaaca aatggtagtg
      241 tc
//
LOCUS       DAZOXR010000582          242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZOXR010000582 DAZOXR010000000
VERSION     DAZOXR010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.597"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>242
                     /locus_tag="ACHUNN_004832"
     CDS             <1..>242
                     /locus_tag="ACHUNN_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0578942.1"
                     /translation="LFLDELPEFERRTLDALREPIESGQIHLSRTRAKITYPARFQLV
                     AAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLD"
BASE COUNT           59 a           73 c           53 g           57 t
ORIGIN      
        1 tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc ttgcgagagc
       61 cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc tatccagccc
      121 gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag ggaaaccata
      181 accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg ccctttctcg
      241 ac
//
LOCUS       DAZOXR010000583          240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZOXR010000583 DAZOXR010000000
VERSION     DAZOXR010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.598"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..>240
                     /locus_tag="ACHUNN_004833"
     rRNA            <1..>240
                     /locus_tag="ACHUNN_004833"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           61 a           55 c           80 g           44 t
ORIGIN      
        1 ggacgaagac tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg
       61 tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga
      121 gctaacgcgt taagtcgacc gcctggggag tacggccgca aggttaaaac tcaaatgaat
      181 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc
//
LOCUS       DAZOXR010000584          236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZOXR010000584 DAZOXR010000000
VERSION     DAZOXR010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.599"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..226
                     /locus_tag="ACHUNN_004834"
     CDS             <1..226
                     /locus_tag="ACHUNN_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578943.1"
                     /translation="VHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEMKRLYAVGS
                     ENGGDGDAFVREEREAMDVVLQALDGEHMS"
BASE COUNT           58 a           49 c           87 g           42 t
ORIGIN      
        1 tgttcacgac gttgttcgtg agctgatgga agaaaagcgc gccagccgtc aggtgacagt
       61 tccggcggtg atgctggaaa aagtgatggc gctggcaggc agtgaaatga agcgcctgta
      121 tgccgtcggg agcgagaacg ggggcgacgg tgacgcattc gtcagggaag aacgcgaagc
      181 aatggacgtt gtgttacagg cgctggacgg ggagcacatg tcatgaatat cagcac
//
LOCUS       DAZOXR010000585          231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZOXR010000585 DAZOXR010000000
VERSION     DAZOXR010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.denovo.600"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
BASE COUNT           58 a           65 c           88 g           20 t
ORIGIN      
        1 cataaaagcc gggcgataag gcggggatgc acagggcgca agggaagccg cagcctgacg
       61 cacacggctg gcgggagttg gagggtgaga ccgcgaaatc tgagcgggtg aacgaccgga
      121 aaatcacggc agacgggaag gagggaccgg gtgaccgcag cggcgaagcg tacccttgca
      181 tccctgccag cccggctaag atggaggcac ggcacagccc gcacacgacg c
//
LOCUS       DAZOXR010000586        22017 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.059, whole genome shotgun sequence.
ACCESSION   DAZOXR010000586 DAZOXR010000000
VERSION     DAZOXR010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.059"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            121..594
                     /gene="dfrA1"
                     /locus_tag="ACHUNN_004835"
     CDS             121..594
                     /gene="dfrA1"
                     /locus_tag="ACHUNN_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0578944.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            689..1213
                     /gene="sat2"
                     /locus_tag="ACHUNN_004836"
     CDS             689..1213
                     /gene="sat2"
                     /locus_tag="ACHUNN_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0578945.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1282..1779
                     /locus_tag="ACHUNN_004837"
     CDS             1282..1779
                     /locus_tag="ACHUNN_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578946.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1840..2211
                     /locus_tag="ACHUNN_004838"
     CDS             1840..2211
                     /locus_tag="ACHUNN_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578947.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2621..2998
                     /locus_tag="ACHUNN_004839"
     CDS             2621..2998
                     /locus_tag="ACHUNN_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578948.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3021..3173
                     /locus_tag="ACHUNN_004840"
                     /pseudo
     CDS             3021..3173
                     /locus_tag="ACHUNN_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3229..4845)
                     /locus_tag="ACHUNN_004841"
     CDS             complement(3229..4845)
                     /locus_tag="ACHUNN_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0578949.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(4846..6372)
                     /locus_tag="ACHUNN_004842"
     CDS             complement(4846..6372)
                     /locus_tag="ACHUNN_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0578950.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(6375..8042)
                     /locus_tag="ACHUNN_004843"
     CDS             complement(6375..8042)
                     /locus_tag="ACHUNN_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0578951.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(8039..10147)
                     /locus_tag="ACHUNN_004844"
     CDS             complement(8039..10147)
                     /locus_tag="ACHUNN_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0578952.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(10134..10955)
                     /locus_tag="ACHUNN_004845"
     CDS             complement(10134..10955)
                     /locus_tag="ACHUNN_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0578953.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(11115..12944)
                     /gene="glmS"
                     /locus_tag="ACHUNN_004846"
     CDS             complement(11115..12944)
                     /gene="glmS"
                     /locus_tag="ACHUNN_004846"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0578954.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(13106..14476)
                     /gene="glmU"
                     /locus_tag="ACHUNN_004847"
     CDS             complement(13106..14476)
                     /gene="glmU"
                     /locus_tag="ACHUNN_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0578955.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(14827..15246)
                     /locus_tag="ACHUNN_004848"
     CDS             complement(14827..15246)
                     /locus_tag="ACHUNN_004848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0578956.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(15267..16649)
                     /gene="atpD"
                     /locus_tag="ACHUNN_004849"
     CDS             complement(15267..16649)
                     /gene="atpD"
                     /locus_tag="ACHUNN_004849"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0578957.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(16676..17539)
                     /gene="atpG"
                     /locus_tag="ACHUNN_004850"
     CDS             complement(16676..17539)
                     /gene="atpG"
                     /locus_tag="ACHUNN_004850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0578958.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(17590..19131)
                     /gene="atpA"
                     /locus_tag="ACHUNN_004851"
     CDS             complement(17590..19131)
                     /gene="atpA"
                     /locus_tag="ACHUNN_004851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0578959.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(19144..19677)
                     /gene="atpH"
                     /locus_tag="ACHUNN_004852"
     CDS             complement(19144..19677)
                     /gene="atpH"
                     /locus_tag="ACHUNN_004852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0578960.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(19692..20162)
                     /gene="atpF"
                     /locus_tag="ACHUNN_004853"
     CDS             complement(19692..20162)
                     /gene="atpF"
                     /locus_tag="ACHUNN_004853"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0578961.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(20224..20463)
                     /gene="atpE"
                     /locus_tag="ACHUNN_004854"
     CDS             complement(20224..20463)
                     /gene="atpE"
                     /locus_tag="ACHUNN_004854"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0578962.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(20510..21325)
                     /gene="atpB"
                     /locus_tag="ACHUNN_004855"
     CDS             complement(20510..21325)
                     /gene="atpB"
                     /locus_tag="ACHUNN_004855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0578963.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(21334..21714)
                     /gene="atpI"
                     /locus_tag="ACHUNN_004856"
     CDS             complement(21334..21714)
                     /gene="atpI"
                     /locus_tag="ACHUNN_004856"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0578964.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         5674 a         5660 c         4963 g         5720 t
ORIGIN      
        1 gtcttgtatt tttaaagaaa gtctatttaa tacaagtgat tatattaatt aacggtaagc
       61 atcagcgggt gacaaaacga gcatgcttac taataaaatg ttaacctctg aggaagaatt
      121 gtgaaactat cactaatggt agctatatcg aagaatggag ttatcgggaa tggccctgat
      181 attccatgga gtgccaaagg tgaacagctc ctgtttaaag ctattaccta taaccaatgg
      241 ctgttggttg gacgcaagac ttttgaatca atgggagcat tacccaaccg aaagtatgcg
      301 gtcgtaacac gttcaagttt tacatctgac aatgagaacg tattgatctt tccatcaatt
      361 aaagatgctt taaccaacct aaagaaaata acggatcatg tcattgtttc aggtggtggg
      421 gagatataca aaagcctgat cgatcaagta gatacactac atatatctag aatagacatc
      481 gagccggaag gtgatgttta ctttcctgaa atccccagca attttaggcc agtttttacc
      541 caagacttcg cctctaacat aaattatagt taccaaatct ggcaaaaggg ttaacaagtg
      601 gcagcaacgg attcgcaaac ctgtcacgcc ttttgtacca aaagccgcgc caggtttgcg
      661 atccgctgtg ccaggcgtta ggcgtcatat gaagatttcg gtgatccctg agcaggtggc
      721 ggaaacattg gatgctgaga accatttcat tgttcgtgaa gtgttcgatg tgcacctatc
      781 cgaccaaggc tttgaactat ctaccagaag tgtgagcccc taccggaagg attacatctc
      841 ggatgatgac tctgatgaag actctgcttg ctatggcgca ttcatcgacc aagagcttgt
      901 cgggaagatt gaactcaact caacatggaa cgatctagcc tctatcgaac acattgttgt
      961 gtcgcacacg caccgaggca aaggagtcgc gcacagtctc atcgaatttg cgaaaaagtg
     1021 ggcactaagc agacagctcc ttggcatacg attagagaca caaacgaaca atgtacctgc
     1081 ctgcaatttg tacgcaaaat gtggctttac tctcggcggc attgacctgt tcacgtataa
     1141 aactagacct caagtctcga acgaaacagc gatgtactgg tactggttct cgggagcaca
     1201 ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct taattcagga
     1261 gttagagagc tggggaagac tatgcgcgat ctgttgaagg tggttctaag cctcgtactt
     1321 gcgatggcat cggggcaggc acttgctgac ctgccaattg ttttagtgga tgaagctcgt
     1381 cttccctatg actactcccc atccaactac gacatttctc caagcaacta cgacaactcc
     1441 ataagcaatt acgacaatag tccatcaaat tacgacaact ctgagagcaa ctacgataat
     1501 agttcatcca attacgacaa tagtcgcaac ggaaatcgta ggcttatata tagcgcaaat
     1561 gggtctcgca ctttcgccgg ctactacgtc attgccaaca atgggacaac gaacttcttt
     1621 tccacatctg gcaaaaggat gttctacacc ccaaaagggg ggcgcggcgt ctatggcggc
     1681 aaagatggga gcttctgcgg ggcattggtc gtcataaatg gccaattttc gcttgccctg
     1741 acagataacg gcctgaagat catgtatcta agcaactagc ctgctctcta ataaaatgtt
     1801 aggcctcaac atctagtcgc aagctgaggg gaaccactag tgtcatacga acctccaaga
     1861 gacggttaca caaacgggta cattgttgat gtcatgtatg acaatcgccc aagtaagtat
     1921 ccagctgtgt tcagaacgta cgtccgaatt ccagactatg tcgaactaga taacgcggag
     1981 gagctggccg acgcaatcat tcgagaggtt gagttaaagg aggcatggct cacaagggtc
     2041 ttggggaact cagccgatcc tgacgcttct gtgggcaagt gggaccggcg cctagcgcag
     2101 tgcggtggta ttgatgagat gtcaagtggg gtgcaggcaa catacttcag tccaaacggt
     2161 cagctggtcg gcttagtggc atacattgat gccgaatgga tggatgaata gctctgacgt
     2221 tacgattcag gtgagacaac cagatgggca ttgcgtacaa gctcagttgc caccgatgtg
     2281 gctacgagtc tgatcttctt tacctaggcc aaggtatggc gatgcttcct gagcaaatag
     2341 tcggaacgtg cacatgctgc gacaatctaa cgacgatttc tataaatgaa agctacggag
     2401 tatgctcact ttgtggggct aaggatcgtg tgatatttac aagctatcag catgaggcaa
     2461 ggccaagaaa tccctgctat ctagagagaa gtttcaaata tcaatgccca agatgccatg
     2521 ccttcagtat ggagcctccg gatctaccgg agatgctttg ggactaatgc tccgctttgc
     2581 ctcgggtatc cgttcgacct atgtttaaga ggaaacgcac gtgtctgccg ccaaagctct
     2641 tgcctatgca ctttgtgaca agttggaaag caaaaaagtt gatgaagtcg tacgaaatct
     2701 tgtttctagc ggctggaata ttagggaagt ggcctgggaa gcttcgtcct ttgatcatga
     2761 aatgccctac tttgacacgg ggcgggactt cgatattgga tttcctgaca cgctgtcatc
     2821 caagattgag tttttgaccg cgaatggatc aatcgtccaa gcacgtgctc gcctgcactt
     2881 caagcaggct ctaatttttg ccaaggcgtc caaacatgtc gccgcgctca agaactcatt
     2941 ggattactac tatggatcgt ctgtaatccc ccctcaaaac cacggaggct atttgtagaa
     3001 aattccatta atatgaactc aaggtgttcc ctacggacga atcagcgatg aaggtggtgt
     3061 acctggctat ccaggcggcg accaagaaat ggacgatgcc gatccgcaac tggaagccgg
     3121 ccatgaaccg ttttatgatc gaatttggtg accgtttaaa tggccacctt taactgaccg
     3181 gcaaaagcac ttacacaaaa gggtttacag gctctttcag tcattccttt aatgcgtaaa
     3241 ttgctctcta accactctct cagcccaccc ttccatatcc tctctcgata cttgcagctc
     3301 ccccaacccc tttggatgat tcacccctct atgcccgtct tgtccatata attgatcaaa
     3361 caaactattt ggccaattca atgagctctt caccactcca cgccgtatct tttcaaaatc
     3421 attccgccat gtttcgctat caacgccact caacactttg gtagaaagca ttttcacccc
     3481 atccgatgca tccacctcta gcaatacaaa ttcactgcca ttacgttgca ccccaacggc
     3541 tttaatcact cttggtgagc catctttaca cagatgtaat cggctacgcc caacttttgg
     3601 caaaaccaag gtttcttcaa aaagcacccg acatgcaaat ttagttttta gaattgaaag
     3661 tagctcatca aaagcagcaa atcgattagc aaaaatagag ttgtaattgg ttgcatcctg
     3721 cttcccaccc acatctgccg ctgctaggac accacctaag tgtggctcat cagtactaac
     3781 caaattacta ttttcacttg ttgttgcttc ttctttttct aaaatagttt ggctactttt
     3841 tcttgccctg cgagattttt caattcgact aggtctacta aaacttatcc atgtcggctc
     3901 agcctctatg actcgtgtgt ctgtgtctat attggctgtt tcctcatcgt ccaactgtag
     3961 atgctcatca tcaacaactg actctgttga aaccgctatg tgctgcgtac taccatcacc
     4021 tgcctccttt tcctgaaaag aggcatggct aatagctgtt gtgctaggaa gcatagcgtt
     4081 gatttctaaa cctactattt cctcaactaa ataatcctta tcctcgttag aagaacgtcc
     4141 ttttacatgt aatttccaac cctgcatggg cggagggtca aagctaaagc accagctttc
     4201 aacgccgttc ttaatttctc tattttgttg atagtgccta aaaatacttt catacgaatc
     4261 cataacatct tgatccgaaa acaaccaaac caaaagctgc actacggccg actgctctaa
     4321 cgccccttta ggaaacgatg aactgggtaa gatccttatc tctaaatgat ctcgctcaac
     4381 ctcatactga acgtcgaact cttgctgtaa agcggtactg ctcaaacagc ttcgacagaa
     4441 ataggagtta attaaaaata atgaccgagc tagctctaac tgtgggatgt gataacgagt
     4501 cttgctatat ggtgtttcag cttcgtaaat aaatgaactc tgcgctccat ccttgttgcg
     4561 tacggaagca aaggacgaga gattaggaaa atcgattatt ttggctcgtt gaagatccga
     4621 tgcttcaaac tcaatcacac gatccgcctt atttatttgc tttgttgtac tgttaaccac
     4681 cttagctcga cttaataaag gcaaatgagt aaatcgagtc catttccgtt cttgcattgg
     4741 attaaaccaa acaaaaatac gccactcctt atgacccgag ttacggaata aatgaccaat
     4801 atgtacaacc tgcacattgt cattaaatgt agctagccta accactcact cttccccata
     4861 aaccatttcc aagaatcttt gtgcttcctc agttatccgc tctttagata acgttgcact
     4921 tcttaataat cgccagcgcc taagctcaac atcttcctgt ttaagcttaa taaaagcttg
     4981 gctaatccgt ctaatttgat aatcttccac actctctgag taacgcttta agcaaagcgc
     5041 taccaaaggc agtttctgta gattttttgc aagagaggtt ccgttaggag tttgctttaa
     5101 cagccagctc gatgtcgctc ttgggtgatc aaggctacta tctagacgct ttatgattct
     5161 taatagttgt cgtacagcaa ttcgatctct ttggttccaa tcaactctag gggcgggtgc
     5221 cagacgctct tgttgatgct gttgattcca gtgaactagc caatccctgt catgtcggta
     5281 aagccaagcg tatagcaccc caccctctaa agactgtctt gccgccttaa ttccttggta
     5341 tttatgcact agttgctgcc agtctttatg cttaacagat aaatcttcag agttaggttg
     5401 cacagactgg ctaacaggtc ttgtcgttat agagtgctca gtaagagcac ttgcctgctg
     5461 tagcgcttct ataaccgtta gttttggcaa taaggcttgc cacacaatac tatgctgtaa
     5521 ataactaaag gcttttctat gcttacggaa tatacttttc agccaacacg tgtccttgtt
     5581 ctctgccaac tttaaatcca gtttctctag tgcctcatca ctaaaagtct gcctcactct
     5641 ctccgccacc aagtcatgac gaatgtgctt gcttttggtt agccctagat cctgcgctaa
     5701 gcgctgataa aacagcgtcc actgctcaag gcttggggaa agctcttgcg ctcgtggagc
     5761 atctaacaga ggggctatat aagcagctag tgctgttaat tgagataggg agtctttggg
     5821 gtagtctgaa agcagctcag tatgacccaa agcccaaaat tgatgtcggt gatcatctac
     5881 agctctatca aagaagacta aagcaccgtg ttttggacaa tatggcaaag cgggcaaata
     5941 ccaatctcgt tgccaaaagg cttccccata cctatttagc tgaagagcaa cgcaatcagg
     6001 gcagtatcta aagcggttat cgctcttaac tctagaagca gcgactccta gcattaaatg
     6061 caccgcacct tgcgcttggt actccattaa ccgaatagct tcgtctcggc gctccttgcc
     6121 tacaaacgga gcatataaag ggaataaggt atgctcataa ataagctgct gaacagcgta
     6181 acgtcctgtt tgatgtagat gtcttgctat cacacctaaa tgcgagggca gacctaaggt
     6241 agcgaccacc ttgcggttgc catacacctc atccaacagc tgcttaggac taacaatccc
     6301 ttgataaacg cctgcccgtg caatagtgct ataaatcagc tcattcgagt acggaacagg
     6361 aaagtttctc atggctaacc tgcttgttta aataagctcg ccatatccac tattacccct
     6421 ttccctttta accgttcatg catggttttt tcaggttggc gttgtgaata gatataacgt
     6481 aaatccgtat caggcaagct atcccagtcg ctgggcttga ctacctttat agccgaaacc
     6541 tttttactct tggagggctt ttctagttct gacactaccg tttcgccttc catcaaccac
     6601 tgcaaaacaa gaggcagtaa cttttgtctt gtcatcgttg gattctggct aaacgctttt
     6661 ttaatagtgg gaattaacag gcttgaatcg taatcctctt tcagcatcag atataaatga
     6721 cgctgatctt cggtatctaa ctcttgaagg gctttttctt ctggtgtttg ttcttgtatc
     6781 gctgcgatat ctagctgaag ttggattaac cgtttatcaa tctcgggaac gactagatca
     6841 gaataacgag caatgcgttc tgggataccc gagcgtaatg cctctagcat ggggtgcaca
     6901 ggctttaact catcttgata cacttgccgc aataaaccag cggtaatacg ctcattgcct
     6961 aaagctagcg cacggagctg agcgagtaca aaaagtttta ctacaatgtc catcactcct
     7021 tggcttagct cataccacac atcacggacc tcatccgata acagcgcatc tttgcgttgt
     7081 aaaagctgta attgccaaag attatccgta aaagcgatcc actcttgatt gggctttcca
     7141 cgttgcgttt gttgtatagg atcccagaat atagctccaa accctgcccc tctacgtgca
     7201 gaccgcaaat cagcctcaaa aatctctcgt gctttagggg taccaatcaa catcactggt
     7261 acgccaataa tattcaccat cgtcacaaaa aagttcagca tctcttgaga tccacccgaa
     7321 cgagagcggc ttaaatgctg aatttcatca ataaccaaca accctaaagc atgtgcattg
     7381 gctatttgcg acatcaaagc caacatggtt tctataccat gacgttttaa gccataacga
     7441 cgctcatagt tcgagcccaa ggctcgatcc aacgctctga aaaaattcaa gcagatttct
     7501 tttagcgaac cattatgcga gcagtctatt ttcaaataca ccacctgctc tacattgagt
     7561 tcacgatggt aaatcacctg aggatacgtg gctagaatac gatgaagaga ggtcgtcttc
     7621 ccactaccag aacaaccaat taacaataag ctttgtgccg tagatcgtgc ctcctcaaag
     7681 cgaaatgtct ccaactctcc cgtttgaaca cgctcataac cattttgtaa atgcttttgt
     7741 aaatctcctg ttttaggatt tctgcctacg tagccacctc gaatcatgac cgaaatacgc
     7801 tcacttagta gcaaatgcgt acctaatggc tgaaaatagt catctggaat acgacaaatg
     7861 gtatgagctc tgataacacg ggacttttgc aagtcagagg aagtaagctg taaagaggat
     7921 ttcagtgatg cagcactatt cactgactct tgtaatggtg gtaaggcctc gataaaagga
     7981 ttatcacgat aagcctctac ccccgtatca cgataaactg cttgaatccg ggtagcactc
     8041 atgactcatc cttttctggt ggatcctgaa ataattcagg cacgtatgtg ggtaggctgt
     8101 aatcttcttg atcatccgct tccactgcgt tgaaaggaat aactttagcc tcatcacctg
     8161 aagagcttgg cttcaaatgc tccgcacgtt ttttacgctc cgaggtcacg gcttcttttt
     8221 tattagtttt aatctgctta atgcgtgttg atttgggctc agtagtactg ggcgttaact
     8281 tattcgcttt ctgaatggtt tgctgaataa acgcctcaag ctccctgcgt ttagttaact
     8341 catcctgctt cgcattggct ttattgtgtt tttcttgtgc ttgtatatcc caaacctccc
     8401 aaaatgagag acctttaaac tgccgactac gttccgtcag attacagcgc caaaatacac
     8461 ggctgccaac ttgcggaaac aaataaatcg tatcaaccag cactgggtca taagccgctt
     8521 ctaaatgttg aggtctagct atatcagtgc tccgctgcaa ccaaccctca cgcagaatct
     8581 ctgaccccga gtaatacaaa ccccacaaat taacgccaaa tgaagaaata gagacctttc
     8641 ggcgaggcag taacgctact cgcaactgct cttgctccac agcccttaaa ctacctgtac
     8701 gatgctgcat accccattgc catagctgga caggaataga cggtaaatct gtaggaaaat
     8761 cagcatctcg atcgtattta tccatcacca gatggttatt tctgaataag atcgtacgca
     8821 aaataatttg tgtgaactca aataccgaca gagatgcatc taacctatag tctgtttcac
     8881 catggctttt gatccgactg ccctctacaa tgccaggtgc aaaggactta aactcggctt
     8941 gtagtgttct aaaagtgctt tccactatgc ctttagcatc gccacgtcta ggtggagcac
     9001 tttccactcg cacattaaaa ctagaaacta aggcttcgac ctgatgactc attaattcgc
     9061 cacggtccgc tagcaacaca tctggcaaac ctacacacgg ccagtctgag ctactaatct
     9121 caatatcatg ctgggcacaa atggccgttt tgtcagagca agcatttaca aaagcctgca
     9181 tcgccaccac ataagacgga ttttcaaagc cgatataaaa gcccgtgatc atccgactaa
     9241 acacatcaat cacaatgtaa agcgttggtc ttcctatgat tttttggcga tcatgatgat
     9301 ccactaaata aatatcagca atcgtggcat caatctcata acgactccca gggcctaacg
     9361 cctgagaagt ggctgtacta cttaagggtc gtacgtcttt tttatatacc cctgctttaa
     9421 ctctagactt taagcgctga gctttagggt attctcgatc ataaaaataa cgaaactgac
     9481 gtagtgttgg gtaatcctct tggggaatgc gaggaaaata ctgagcaaac aagtccacaa
     9541 atcgtctata ggcaacggtg gtctttgtac ctttttgatt taacaggtgc ttttctatgg
     9601 tcaacctaaa aaggcgttca atctcgggcg ttaccttggt tccttcaccc ttaccatatt
     9661 ctctggctcg gccaatcttt gctgttcctg tcgctgaacg tctttcccct ggtgcaccgc
     9721 tgtttttgta gtcaggaatt aatgcattag gcgtttgacc acgctgccag taacggcgta
     9781 acaacttata aactgtagcc ttagtaacct tatgttcttg gaccacatgc tcaacgagtt
     9841 cgcttctgac tttagggtcg aaacgatcct tactattaat aataggaaga atttttcgat
     9901 aatcctcctc gcgcttctgg aagctgacag aatctacaga cggctcttct aaatcaagat
     9961 gtacataagg gtcatcagtt ctaactaagc ggccttcatc taaatactgc attagcaaca
    10021 actcagccct agcttgtgga actcctttat cagcgcttat ttgcatccag acaacttggc
    10081 catcctctat agccaaaatg cgatacggat cattatcaaa tagcacaacc tcattaattt
    10141 gccacatagc gcaactcctc catattcact acttggctaa tacagagatc tgcacactta
    10201 tttgccctga aagacttata aatattgaac ttaataaaac catttgcggt taaggctcgt
    10261 atctcactca atgttttgcc taactccaaa tcataagcaa tatcaacctg cttacagaca
    10321 ttgataatgt tttcatctcc tttttcttgc aggatatggg ccaatgggga tagttgtgct
    10381 aaaagctccg cagaaacttc ttctgttttc actgaataaa gccattcaat attttctttt
    10441 actacgggat ttatttcttt atcagtaaaa atgaaccaag gaatttgctt ttgctgccaa
    10501 tagcgacgct ctagttctag tttttctaag gtacgctcgt cttgtaaggc tgctgcaggt
    10561 ttgacttgaa tagcaaactg ctcaaaagga ccatctttgc agtccactaa aaaatcagta
    10621 gacataacct gatctacacc acgaataaca ggatgcttaa taccactatc tattgcaatc
    10681 tgcctggtat cactaggtaa taaggggaac tgctcgcgta tatctagcac gctgctctcc
    10741 cactcaagac tgagaaaaac agcaagctct aagtcagata gcaaatgatg gactcgtccc
    10801 gtcttatgag aataaatacg gtgggaacga cctgaagaag gaacttcttg tactgttagc
    10861 catggaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg acgggcaatt
    10921 tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca
    10981 cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc
    11041 atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacaa gccctttttt
    11101 atctatgata gtgattactc aaccgtaacc gattttgcca ggttacgcgg ctggtcaacg
    11161 tcggtgcctt tgatcagcgc gacatggtaa gccagcagct gcagcggaac ggtgtagaag
    11221 atcggtgcaa tcacctcttc cacatgcggc atctcgatga tgtgcatgtt atcgctactt
    11281 acaaaacccg catcctgatc ggcgaagaca tacaattggc cgccacgcgc gcgaacttct
    11341 tcaatgttgg atttcagttt ttccagcaat tcgttgttcg gtgcaacgac gataaccggc
    11401 atatcggcat caataagcgc cagcggaccg tgtttcagtt caccagcagc gtaggcttca
    11461 gcgtgaatgt aggagatctc tttcagcttc aatgcgcctt ccagtgcgat tgggtactga
    11521 tcgccacggc ccaggaacag tgcgtgatgt ttgtcagaga aatcttctgc cagagcttca
    11581 atgcgtttgt cctgagatag catctgctca atacggctcg gcagcgcctg cagaccatgc
    11641 acaatgtcat gttcaatgga ggcatccaga cctttcaggc gagacagctt cgccaccagc
    11701 atcaacagca cagttaactg agtggtgaat gctttagtgg acgccacgcc gatttccgta
    11761 cccgcgttgg tcatcagcgc cagatcggat tcgcgcacca gagaagaacc cggaacgtta
    11821 cagattgcca gcgaaccaag gtaacccagc tctttcgaca gacgcaggcc cgccagggta
    11881 tccgcggttt cgccagactg tgacaaggtg atcatcaggc tgttacgacg cacggcagat
    11941 ttacgatagc ggaattcaga ggcgatttcg acgtcgcacg gaatacctgc cagcgattca
    12001 aaccagtagc gggaaaccat accggagtta taagaagtac cacaggcgag gatctgaata
    12061 tgctcaacct tcgacagcag ttcgtcggcg ttcggtccca gctcgcttaa atcaacctga
    12121 ccgtggctga tgcgcccggt aagggtgttt tttatcgcgt tcggctgttc gtagatctct
    12181 ttctgcatgt agtgacggta aataccttta tcgcccgcgt catattgcag attggattcg
    12241 atatcctgac gttttacttc cgcgccagtt ttatcgaaga tgtttaccga acggcgagtg
    12301 atttccgcaa tatcgccctc ttcaaggaag ataaagcgac gggtcaccgg caacagcgcc
    12361 agctggtcag aagcgataaa gttttcgccc atacccaggc caatcaccag cggactacca
    12421 gaacgtgccg ccagcagggt atccgggtga cgggagtcca tgatcactgt accgtacgca
    12481 ccgcgcagct gcgggatagc acgcagaaca gcctcacgca gagtcccgcc ttgtttcagc
    12541 tcccagttca ccagatgggc aatcacttcg gtgtcggttt cagaaacgaa ggtatagcca
    12601 cgcgctttta actcttcacg cagcggttca tggttttcga tgatgccgtt atgcaccacc
    12661 acaatgtgtt cagaaacatg cggatgcgcg ttagcttctg aaggttcacc gtgggtcgcc
    12721 cagcgagtgt gagcaatacc ggtgccgcca tccagaggat gttcttccgc tgcctgagcc
    12781 agcatctgga ctttaccgag gcgacgcagg cgggtcatat gaccttccgc atcaacaacg
    12841 gccagaccgg cagagtcata tccgcggtat tccagacgac gtaaaccttc aagaaggatt
    12901 tctgctacat cacgttgcgc gatcgcgcca acaattccac acatagtttt tgattccgat
    12961 ttatatcgtt gtcggtcaac ctgtatgccc gttttttcgg gcgccccgag ccttgtagag
    13021 agtggggtta tttttatagt tactgcttgt gggagggaga ttatgttatc tcctcatccc
    13081 agatgaccgg attacccggc cagaattact ttttctttac cggacgacgc cagccttctt
    13141 tctgagtctg cggcacacgg ctgatagcta atgcattttc gccgacatta cgcgtcacag
    13201 ttgtacccgc agcaatggtc gcgcctttgc ctactgttac cggggccacc agctgagtgt
    13261 cggaaccgac aaacacatcg tcgccgataa tggtcttaaa tttattcgca ccatcgtagt
    13321 tgcaggtaat ggttcccgcg ccgatgttaa cgttatcgcc aatttccgca tcgcccaggt
    13381 aagtcagatg cccgactttc gagcctttac ccagacgcgc ttttttcatc tcaacgaagt
    13441 taccaacgtg tgcaccttcc agcaactcag caccaggacg caaacgagca aacgggccaa
    13501 tagtacaggc cgctgccaga ttcgcgtctt ccacaacggt atacggactg atttcgcaat
    13561 catcgccaat cacgctgttt ttaatcacgc aaccggtgcc gattttcacg cgatgaccga
    13621 gagtcacgtt gccctcgatg ataacgttag tatcaatttc aacatcgcgc ccgtgagtaa
    13681 gcgtaccgcg cagatcaaaa cgcgccggat cgcgcagcat aacgcctgct aacagcagtt
    13741 tttcagcctg ttcggactgg taaacgcgct ccagacggga gagttgcagg cggttattca
    13801 caccttctac ttcgcttaaa cgttgcggat gaacggcgac gatttcacgc ccttcctgat
    13861 acgccagcgc aataatgtcg gtgatgtagt attcgccctg agcattattg ttggtcagct
    13921 tcgccagcca gcgtttcata tctgcgccgt tagcgatcag aatgccggtg ttgatctcct
    13981 gaatctgacg ctgctcgtcg gtggcatctt tgtgctcaac aatgccggta actttgccat
    14041 tttcacgggt gatacgtcca taaccggtcg gatcatccag tttcaccgtc agcagaccaa
    14101 tgccaccctg cggtttagca tcacgcaggc gctggagtgt ttcgacagag atcagcggca
    14161 cgtcgccgta gagcattaaa atgtcttcat catcggcaaa gaaaggtgcg gcctgctgca
    14221 ttgcatgacc cgtacccagc tgctctgcct gaagcaccca gttaaggttg tcgtctttca
    14281 gcgcctgttt aagcagatcg ccgccgtgac cgtacaccaa gtgaacgtgc gctgcgccta
    14341 attcattcgc agcatcaatg acatgctgaa ccatcgcttt cccggcaagg gtatgcagca
    14401 ctttcggaag atcggaatac atgcgcgtgc ctttgcctgc ggcaaggatc actacgctca
    14461 tagcattatt caacatacgc gtcctgactg taatttgaga acgaatttaa accgcttcac
    14521 cttgaaaaaa ctacattttt ttcatcgtga aatggacaga ggataaattg ttcaataacg
    14581 gattatcccc gctgacgaaa gtgccatttt cgaccatcgt cacgcctttt gtctgcagaa
    14641 aattaaggca gtaccttcat ttaagcgtca ggtggatgtt tttgctctta tttcgatcaa
    14701 tgaataaacg ggaaaacagg ggtgttttat atcagcagga tctatgtgaa cgctcttcag
    14761 gacggatcac gcgtaaaaaa aagccagcct gtttccagac tggcttttgt gcttttcaag
    14821 ccggtgttac atcgcttttt tggtcaactc gataacgcgc agctgcgcga tcgctttggc
    14881 cagttccgca gacgcctgag cgtaatctac gtcgccgtga gagctgctaa tgtgctcttc
    14941 agccttacgt ttcgcttcca tggctcgcgc ttcgtcgaga tcctggccgc gaattgcggt
    15001 gtcggccaga acggtcacgt tgccaggctg cacttcaaga atgccgccag acagatagat
    15061 aaactcttcg tgaccgtgct gtttcacgat gcgaatcata ccaggcttaa tggcggtgag
    15121 cagcggtgcg tggccagggt agatccccag ttcaccttcg ctacccgtta cctggatttt
    15181 ctcgaccaga ccagagaaca tttgttgctc tgcgctgacg acgtccaggt ggtaagtcat
    15241 tgccatatca ccctccgatt aaggcgttaa agttttttgg ctttttccac agcttcttcg
    15301 atggaaccga ccatgtagaa cgcctgctcc ggcaggtgat cgtattcgcc ttccatgatg
    15361 cctttaaagc cacggatggt gtctttcagg gagacgtatt tacccggaga accggtgaat
    15421 acttctgcca cgaagaacgg ctgggacagg aagcgctgga tcttacgagc acgcgctacc
    15481 accagtttgt cttcttcaga cagttcatcc atacccaaga tggcgatgat gtctttcagt
    15541 tcctgataac gttgcaggat ggactgaacg ccacgcgcgg tgtcgtagtg ttcctgacca
    15601 accaccagcg ggtccagctg acggctggtg gagtccagcg ggtcaacggc cgggtagata
    15661 cccagagacg cgatctgacg gctcagtacc acggttgcgt caaggtgcgc aaaggtggtt
    15721 gccggagacg ggtcagtcaa gtcatccgca ggtacgtata ccgcctgtac ggaggtgata
    15781 gaaccagttt tggtggaggt gatacgttcc tgcagaacgc ccatctcttc cgccagggtc
    15841 ggctgataac ctaccgctga aggcatacgg cccagcagtg cggatacttc cgtaccggcc
    15901 agggtgtaac gatagatgtt gtcaacgaac agcagaacgt cacgaccttc gtcacggaat
    15961 ttctcagcca tggtcagacc ggtcagagca acgcgcagac ggtttcccgg cggctcgttc
    16021 atctggccat acaccaggga tactttgtcg ataacgttgg agtcggtcat ttcgtggtag
    16081 aagtcgttac cctcacgagt acgttcacct acgcccgcaa acacagagta accggagtgc
    16141 tcgatcgcga tgttacgaat gagctccatc atgtttacgg ttttacctac acccgcacca
    16201 ccgaacagac caactttacc gcccttagcg aacggacaca tcaggtcgat aactttgata
    16261 ccggtttcca gcagttcctg agagtttgac agctcttcgt aggaaggtgc tgcgcggtga
    16321 atcgcccaac gctcttcttc accgatctcg cctttcatgt cgaccggttc acccagtacg
    16381 ttcatgatac ggcccagagt cgctttacct accgggactt cgatcgggtg ttcgaggtct
    16441 tttacatcca gaccgcgacg cagaccgtcg gaggaaccca ttgcgatggt acgcacgata
    16501 ccgccgccga gctgctgctg aacttccagc accagacgct cattaccatt ttgcacctca
    16561 agagcatcgt acacgcgcgg tacggcatcc tgagggaatt cgacgtcaac tacggcgccg
    16621 attacctgga caatctttcc agtagccatc ttaaatcctc tacgaaataa cctgtttaaa
    16681 ccgcggcggc ccccgagacg atctcggtga gttcctgagt aatgctggcc tgacgagctt
    16741 tgttgtatac caactgcagc tctttaatca ggctgccgcc attgtcggtc gcggctttca
    16801 tcgccaccat acgggcggcc tgctcgctgg ccaggttttc aaccacgccc tgataaacct
    16861 gagattcgac ataacgacgc agcagggtat ccagcaacgc cttcggatcg ggttcgtaca
    16921 ggtaatccca ggatttatgt ttcagatcat catcatctga tgccggtaac ggcagcagct
    16981 ggctgatggt cggaacctga gacatggtgt taataaattt gttgctgaca atgtaaagct
    17041 tgtccagacg gccttcgtcg taagcctgca acatcacttt taccggaccg atcagttcgg
    17101 acagggaagg gttatccccc atgccggtga cctgggcaac aacattgccg cccacggagt
    17161 tgaagaacga cacgcctttc gagccgatca ttgcgaggtc gcattgaacg cttttgtcgg
    17221 tccaggtctt catttccgcc agtagttttt tgaacaggtt aatgttcaaa ccaccgcaca
    17281 aaccacggtc ggtcgacacc accaggtagc ccacgcgttt aacgtcgcgg tcttccaggt
    17341 aagggtgctt atattccaga ttaccgtgtg caaggtgacc aatcactttg cgcatggttt
    17401 ctgcataagg acggctggcc gccatgcgat cctgcgattt acgcattttg gaagcggcga
    17461 ccatctccat cgctttagtg atcttttgcg tgttctggac gcttgcgatc ttactacgta
    17521 tctcttttgc gccggccatg agcttctcct caatgccttg cggcctgccc taaggcaagc
    17581 cgccagacgt taccaggatt gggttgcttt gaaggaatcg aggatgcctt tcagcttgcc
    17641 ttcgatttcg tcgttgtagc caccggtctg gttgatctct tgcatcaacg gagcgtgatc
    17701 acggtcgacg taagccagca gagcggcttc gaagctgcca attttcgaca gttcaacatc
    17761 cgccaggtaa ccacgttctg ctgcgaacag aaccagagac tgctgcgcaa cggacatcgg
    17821 cgcatactgt ttctgtttca gcagttcggt cactttctga ccgtggtcaa gctgcttacg
    17881 tgttgcatcg tcaaggtcgg atgcaaactg agagaacgct gccagttcac gatactgtgc
    17941 cagagcggta cggataccac cggacagttt tttcatgatc ttggtctgtg ctgcaccacc
    18001 aacacgggat acggaaatac ccgggttaac cgcaggacga ataccggcgt tgaacaggtt
    18061 ggtttccagg aagatctgac catcggtaat ggagattacg ttggtcggaa cgaacgcaga
    18121 aacgtcaccc gcctgagttt cgataatcgg cagtgcggtc agagaaccgg ttttcccttt
    18181 cacttcacct ttggtgaagg cttcaacgta ttcggcgtta acacgtgcag cacgctccag
    18241 cagacgagag tggaggtaga aaacgtcgcc cgggaatgct tcacgtcctg gcggacgacg
    18301 gagcagcagg gagatctgac ggtaagcaac agcctgttta gacaggtcat cgtaaatgat
    18361 cagcgcatct tcaccgcggt cacggaagta ttcgcccatt gcgcaaccgg catacggtgc
    18421 caggtattgc agtgcagcgg attcagacgc ggttgctacc acaacgatgg tgttagccag
    18481 tgcgccgtgc tcttccagtt tacgtaccac gttagaaatg gtggacgctt tctggccgat
    18541 agcgacatag atacatttga taccggaatc gcgctggttg atgatggcat cgatagccag
    18601 tgcggtttta cctgtctgac ggtcaccgat gatcaattca cgctgaccac gaccgattgg
    18661 gatcatggag tcaacggctt tataaccggt ctgtaccggc tgatctacgg actgacgttc
    18721 gataacgccc ggagcgattg cttctacagc agagaagccg tcgtgatcca gcggaccttt
    18781 accgtcgatt ggtgcaccca gagtgttaac cacacggccc agcaggccac ggccaaccgg
    18841 aacttccagg atacggccag tacacttaac tttcatgcct tcggcaaggt cagcgtacgg
    18901 acccataaca accgcaccta cagagtcgcg ctcaaggttc agtgcgatag cgtaacggtt
    18961 acccggcagg gagatcattt caccctgcat acaatcggcc aggccgtgaa tgcggataac
    19021 accgtcactt acagaaacaa tagtaccttc gttgtgagct tcactcacaa cattgaactg
    19081 agcaatgcgc tgcttgatca gttcgctgat ttcggtggaa ttcagttgca tgctccagtc
    19141 cccttaagac tgcaagacgt ctgcaaggcg ctcaagacga ccgcgtacgc tgccatcaat
    19201 gaccatatca cccgctcgga tgataacgcc tgccattaca gacttatcga ttttgcaatt
    19261 cagcttaact ttgcgtgaca gacgtttttc catcgcagca gaaattttcg cgagctgttg
    19321 ttcactcagt gcggcagcgg aaatgacgtc tacctcagcg gtagcctcac tcacggcacg
    19381 aaggtgaata aactgctcca gaacatccgg gagcgcgtta agacgaccat tttcagccat
    19441 tacccgaatc aggttctgac cgttttcgtc cagttgctca ccacaaactg cgataaacga
    19501 ctcggcgagc gtttctggcg caagcgcgcc agagagaagc tctgccattt gttcgttttt
    19561 ggttacctcg gcggcaaacg ccagcatgtc ctgccagcgt tctacacttt ggtgttcgac
    19621 ggcaaagtca aaagctgctt tggcgtaggg gcgagctacc gtaataaatt cagacatcag
    19681 cccctccctc cttacagttc agcgacaagt ttatccacga tgtcgctgtt agcagcttca
    19741 tccacggaac gttcgatgat cttctcggcg ccagcaacag ccaggatagc aacttgctta
    19801 cgcagctctt cacgggcacg tttacgctcg gcttcaattt ctgcctgcgc ctgggccacg
    19861 attttagtac gttcctgttc tgcctcagct ttcgcttcgt ccagaatctg cgagcggcgt
    19921 ttgttcgcct gctcgatgat tacctgggct tccgctttcg cttttttcag ctggtcggtc
    19981 gcgctggcct ttgcaaggtc aaggtcctta tgtgctcgtt ctgcggaagc aaggccgtca
    20041 gcaatttctt tttgacgttt ttcgatggct gccattaatg gcggccatac gtacttcatg
    20101 cagaacagaa cgaacaggac aaacgcgatg gcctggccga ggattgttgc gttaagattc
    20161 acagcacaat gcctctattt agttaacgtt ctgatattgc tctttaaata aaagcaacgc
    20221 ttactacgcg acagcgaaca tcacgtacag acccagacct acagcgatca tcgggatagc
    20281 atccaccaga cccataacga taaagaactg agtacgcagc agaggaatca gatcaggttg
    20341 acgcgctgcg ccttccagga atttaccccc gaggatgccg ataccgatcg cagcaccgat
    20401 tgccgccaga cccatcatca cagcggcagc catgtacagc agatccatat tcaggttttc
    20461 catgacagtc tccagtttgt ttcagttaaa acgtagtagt gttggtaaat taatgttctt
    20521 cagacgccat cgacagatag acgatcgtca gaaccatgaa gatgaaggct tgcagcgtaa
    20581 tgatcaggat gtggaaaatg gcccacggca cattcaggat ccactgtgac caccacggca
    20641 acagaccagc aatcagaatg aaaatcagct caccggcata catgttaccg aacagtcgca
    20701 aaccgagtga aactggtttg gacagcaggc ttaccccttc aaggattaag ttgacaggaa
    20761 tgaacgccca gtgattgaac ggctgcagcg tcaactcttt cgtgaagccg ccgatgcctt
    20821 tcattttgat gctgtagaac agaatcagga taaatacgcc cagtgccata gacagcgtta
    20881 cgttcacgtc cgcagacgga accacacgca gtgcaggcag acccagtaca tgttcagcaa
    20941 tgtacggcag caggtcgata ggcagtaaat ccatcaggtt catcaggaat acccagacga
    21001 agatcgtcag ggccagcgga gcaatcagct tgcttttgcc atggtacatg tctttcacgc
    21061 taccattaac aaagccgatc accagctcaa tcgcggtctg aaacttacct ggcacaccgc
    21121 tggtcgcctt tttggctacg ctacggaata aaaccaggaa caacagaccc agcaccaccg
    21181 agaagaacat ggagtcaata ttgattgtcc agaaggtggc tggggggttt tgtggatcca
    21241 ccagcgagaa tgtacgcagg tccagctgaa ggttattcag gtggtgtcct atgtaatcct
    21301 gcggcgtcat attttctgaa gccatgatgc cttttaccct ttgttgttaa ttacagccgg
    21361 tgccagtatc tgaaccacca gcaccaaaac ccacgtaacg atcagcggca agaataccgc
    21421 ctttaaaacc gccaacgcca ccaccagtaa caccaacatc gccagaactt tgaaagcttc
    21481 gccaaatgcg aatgtccagg ccacccggcc tttcgctggt gtatacgcct ggtgacgcca
    21541 ggcaaatatc ataaacaaaa cgttaggcag aaagactgcc aggcccccgc ttattgcaga
    21601 gacgccccag aaggggtctt tgaggctgaa cagcaatcca cttgctatca ccaccagtaa
    21661 ctgaacgagc agaagcttcc gagcaacgtt tcgactcacg agcgacacag acatcacgtt
    21721 tttcactcct gctcccttcg aggtatgccg cgtgtcgtat aaaactttct ttaaggctta
    21781 gagtcaagca tcaaaaagcg gtcaaattat acggtgcgcc cccgtgattt caaacaataa
    21841 gtagccaaaa ggtgaataaa tgtttaaata tttttccagt gcatacaatt gcgacttttc
    21901 cgctaaccct gttcgatcat gaaaaactgt aaataacgcg taaacactag tgataaagcg
    21961 tgcttcagat cacatattgc gcatgttcgc gcacagtata tttatttact tggcaaa
//
LOCUS       DAZOXR010000587        22017 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.060, whole genome shotgun sequence.
ACCESSION   DAZOXR010000587 DAZOXR010000000
VERSION     DAZOXR010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.060"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            121..594
                     /gene="dfrA1"
                     /locus_tag="ACHUNN_004857"
     CDS             121..594
                     /gene="dfrA1"
                     /locus_tag="ACHUNN_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0578965.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            689..1213
                     /gene="sat2"
                     /locus_tag="ACHUNN_004858"
     CDS             689..1213
                     /gene="sat2"
                     /locus_tag="ACHUNN_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0578966.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1282..1779
                     /locus_tag="ACHUNN_004859"
     CDS             1282..1779
                     /locus_tag="ACHUNN_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578967.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1840..2211
                     /locus_tag="ACHUNN_004860"
     CDS             1840..2211
                     /locus_tag="ACHUNN_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578968.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2621..2998
                     /locus_tag="ACHUNN_004861"
     CDS             2621..2998
                     /locus_tag="ACHUNN_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578969.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3021..3173
                     /locus_tag="ACHUNN_004862"
                     /pseudo
     CDS             3021..3173
                     /locus_tag="ACHUNN_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3229..4845)
                     /locus_tag="ACHUNN_004863"
     CDS             complement(3229..4845)
                     /locus_tag="ACHUNN_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0578970.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(4846..6372)
                     /locus_tag="ACHUNN_004864"
     CDS             complement(4846..6372)
                     /locus_tag="ACHUNN_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0578971.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(6375..8042)
                     /locus_tag="ACHUNN_004865"
     CDS             complement(6375..8042)
                     /locus_tag="ACHUNN_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0578972.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(8039..10147)
                     /locus_tag="ACHUNN_004866"
     CDS             complement(8039..10147)
                     /locus_tag="ACHUNN_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0578973.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(10134..10955)
                     /locus_tag="ACHUNN_004867"
     CDS             complement(10134..10955)
                     /locus_tag="ACHUNN_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0578974.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(11115..12944)
                     /gene="glmS"
                     /locus_tag="ACHUNN_004868"
     CDS             complement(11115..12944)
                     /gene="glmS"
                     /locus_tag="ACHUNN_004868"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0578975.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(13106..14476)
                     /gene="glmU"
                     /locus_tag="ACHUNN_004869"
     CDS             complement(13106..14476)
                     /gene="glmU"
                     /locus_tag="ACHUNN_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0578976.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(14827..15246)
                     /locus_tag="ACHUNN_004870"
     CDS             complement(14827..15246)
                     /locus_tag="ACHUNN_004870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0578977.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(15267..16649)
                     /gene="atpD"
                     /locus_tag="ACHUNN_004871"
     CDS             complement(15267..16649)
                     /gene="atpD"
                     /locus_tag="ACHUNN_004871"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0578978.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(16676..17539)
                     /gene="atpG"
                     /locus_tag="ACHUNN_004872"
     CDS             complement(16676..17539)
                     /gene="atpG"
                     /locus_tag="ACHUNN_004872"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0578979.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(17590..19131)
                     /gene="atpA"
                     /locus_tag="ACHUNN_004873"
     CDS             complement(17590..19131)
                     /gene="atpA"
                     /locus_tag="ACHUNN_004873"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0578980.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(19144..19677)
                     /gene="atpH"
                     /locus_tag="ACHUNN_004874"
     CDS             complement(19144..19677)
                     /gene="atpH"
                     /locus_tag="ACHUNN_004874"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0578981.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(19692..20162)
                     /gene="atpF"
                     /locus_tag="ACHUNN_004875"
     CDS             complement(19692..20162)
                     /gene="atpF"
                     /locus_tag="ACHUNN_004875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0578982.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(20224..20463)
                     /gene="atpE"
                     /locus_tag="ACHUNN_004876"
     CDS             complement(20224..20463)
                     /gene="atpE"
                     /locus_tag="ACHUNN_004876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0578983.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(20510..21325)
                     /gene="atpB"
                     /locus_tag="ACHUNN_004877"
     CDS             complement(20510..21325)
                     /gene="atpB"
                     /locus_tag="ACHUNN_004877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0578984.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(21334..21714)
                     /gene="atpI"
                     /locus_tag="ACHUNN_004878"
     CDS             complement(21334..21714)
                     /gene="atpI"
                     /locus_tag="ACHUNN_004878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0578985.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         5673 a         5660 c         4964 g         5720 t
ORIGIN      
        1 gtcttgtatt tttaaagaaa gtctatttaa tacaagtgat tatattaatt aacggtaagc
       61 atcagcgggt gacaaaacga gcatgcttac taataaaatg ttaacctctg aggaagaatt
      121 gtgaaactat cactaatggt agctatatcg aagaatggag ttatcgggaa tggccctgat
      181 attccatgga gtgccaaagg tgaacagctc ctgtttaaag ctattaccta taaccaatgg
      241 ctgttggttg gacgcaagac ttttgaatca atgggagcat tacccaaccg aaagtatgcg
      301 gtcgtaacac gttcaagttt tacatctgac aatgagaacg tattgatctt tccatcgatt
      361 aaagatgctt taaccaacct aaagaaaata acggatcatg tcattgtttc aggtggtggg
      421 gagatataca aaagcctgat cgatcaagta gatacactac atatatctag aatagacatc
      481 gagccggaag gtgatgttta ctttcctgaa atccccagca attttaggcc agtttttacc
      541 caagacttcg cctctaacat aaattatagt taccaaatct ggcaaaaggg ttaacaagtg
      601 gcagcaacgg attcgcaaac ctgtcacgcc ttttgtacca aaagccgcgc caggtttgcg
      661 atccgctgtg ccaggcgtta ggcgtcatat gaagatttcg gtgatccctg agcaggtggc
      721 ggaaacattg gatgctgaga accatttcat tgttcgtgaa gtgttcgatg tgcacctatc
      781 cgaccaaggc tttgaactat ctaccagaag tgtgagcccc taccggaagg attacatctc
      841 ggatgatgac tctgatgaag actctgcttg ctatggcgca ttcatcgacc aagagcttgt
      901 cgggaagatt gaactcaact caacatggaa cgatctagcc tctatcgaac acattgttgt
      961 gtcgcacacg caccgaggca aaggagtcgc gcacagtctc atcgaatttg cgaaaaagtg
     1021 ggcactaagc agacagctcc ttggcatacg attagagaca caaacgaaca atgtacctgc
     1081 ctgcaatttg tacgcaaaat gtggctttac tctcggcggc attgacctgt tcacgtataa
     1141 aactagacct caagtctcga acgaaacagc gatgtactgg tactggttct cgggagcaca
     1201 ggatgacgcc taacaattca ttcaagccga caccgcttcg cggcgcggct taattcagga
     1261 gttagagagc tggggaagac tatgcgcgat ctgttgaagg tggttctaag cctcgtactt
     1321 gcgatggcat cggggcaggc acttgctgac ctgccaattg ttttagtgga tgaagctcgt
     1381 cttccctatg actactcccc atccaactac gacatttctc caagcaacta cgacaactcc
     1441 ataagcaatt acgacaatag tccatcaaat tacgacaact ctgagagcaa ctacgataat
     1501 agttcatcca attacgacaa tagtcgcaac ggaaatcgta ggcttatata tagcgcaaat
     1561 gggtctcgca ctttcgccgg ctactacgtc attgccaaca atgggacaac gaacttcttt
     1621 tccacatctg gcaaaaggat gttctacacc ccaaaagggg ggcgcggcgt ctatggcggc
     1681 aaagatggga gcttctgcgg ggcattggtc gtcataaatg gccaattttc gcttgccctg
     1741 acagataacg gcctgaagat catgtatcta agcaactagc ctgctctcta ataaaatgtt
     1801 aggcctcaac atctagtcgc aagctgaggg gaaccactag tgtcatacga acctccaaga
     1861 gacggttaca caaacgggta cattgttgat gtcatgtatg acaatcgccc aagtaagtat
     1921 ccagctgtgt tcagaacgta cgtccgaatt ccagactatg tcgaactaga taacgcggag
     1981 gagctggccg acgcaatcat tcgagaggtt gagttaaagg aggcatggct cacaagggtc
     2041 ttggggaact cagccgatcc tgacgcttct gtgggcaagt gggaccggcg cctagcgcag
     2101 tgcggtggta ttgatgagat gtcaagtggg gtgcaggcaa catacttcag tccaaacggt
     2161 cagctggtcg gcttagtggc atacattgat gccgaatgga tggatgaata gctctgacgt
     2221 tacgattcag gtgagacaac cagatgggca ttgcgtacaa gctcagttgc caccgatgtg
     2281 gctacgagtc tgatcttctt tacctaggcc aaggtatggc gatgcttcct gagcaaatag
     2341 tcggaacgtg cacatgctgc gacaatctaa cgacgatttc tataaatgaa agctacggag
     2401 tatgctcact ttgtggggct aaggatcgtg tgatatttac aagctatcag catgaggcaa
     2461 ggccaagaaa tccctgctat ctagagagaa gtttcaaata tcaatgccca agatgccatg
     2521 ccttcagtat ggagcctccg gatctaccgg agatgctttg ggactaatgc tccgctttgc
     2581 ctcgggtatc cgttcgacct atgtttaaga ggaaacgcac gtgtctgccg ccaaagctct
     2641 tgcctatgca ctttgtgaca agttggaaag caaaaaagtt gatgaagtcg tacgaaatct
     2701 tgtttctagc ggctggaata ttagggaagt ggcctgggaa gcttcgtcct ttgatcatga
     2761 aatgccctac tttgacacgg ggcgggactt cgatattgga tttcctgaca cgctgtcatc
     2821 caagattgag tttttgaccg cgaatggatc aatcgtccaa gcacgtgctc gcctgcactt
     2881 caagcaggct ctaatttttg ccaaggcgtc caaacatgtc gccgcgctca agaactcatt
     2941 ggattactac tatggatcgt ctgtaatccc ccctcaaaac cacggaggct atttgtagaa
     3001 aattccatta atatgaactc aaggtgttcc ctacggacga atcagcgatg aaggtggtgt
     3061 acctggctat ccaggcggcg accaagaaat ggacgatgcc gatccgcaac tggaagccgg
     3121 ccatgaaccg ttttatgatc gaatttggtg accgtttaaa tggccacctt taactgaccg
     3181 gcaaaagcac ttacacaaaa gggtttacag gctctttcag tcattccttt aatgcgtaaa
     3241 ttgctctcta accactctct cagcccaccc ttccatatcc tctctcgata cttgcagctc
     3301 ccccaacccc tttggatgat tcacccctct atgcccgtct tgtccatata attgatcaaa
     3361 caaactattt ggccaattca atgagctctt caccactcca cgccgtatct tttcaaaatc
     3421 attccgccat gtttcgctat caacgccact caacactttg gtagaaagca ttttcacccc
     3481 atccgatgca tccacctcta gcaatacaaa ttcactgcca ttacgttgca ccccaacggc
     3541 tttaatcact cttggtgagc catctttaca cagatgtaat cggctacgcc caacttttgg
     3601 caaaaccaag gtttcttcaa aaagcacccg acatgcaaat ttagttttta gaattgaaag
     3661 tagctcatca aaagcagcaa atcgattagc aaaaatagag ttgtaattgg ttgcatcctg
     3721 cttcccaccc acatctgccg ctgctaggac accacctaag tgtggctcat cagtactaac
     3781 caaattacta ttttcacttg ttgttgcttc ttctttttct aaaatagttt ggctactttt
     3841 tcttgccctg cgagattttt caattcgact aggtctacta aaacttatcc atgtcggctc
     3901 agcctctatg actcgtgtgt ctgtgtctat attggctgtt tcctcatcgt ccaactgtag
     3961 atgctcatca tcaacaactg actctgttga aaccgctatg tgctgcgtac taccatcacc
     4021 tgcctccttt tcctgaaaag aggcatggct aatagctgtt gtgctaggaa gcatagcgtt
     4081 gatttctaaa cctactattt cctcaactaa ataatcctta tcctcgttag aagaacgtcc
     4141 ttttacatgt aatttccaac cctgcatggg cggagggtca aagctaaagc accagctttc
     4201 aacgccgttc ttaatttctc tattttgttg atagtgccta aaaatacttt catacgaatc
     4261 cataacatct tgatccgaaa acaaccaaac caaaagctgc actacggccg actgctctaa
     4321 cgccccttta ggaaacgatg aactgggtaa gatccttatc tctaaatgat ctcgctcaac
     4381 ctcatactga acgtcgaact cttgctgtaa agcggtactg ctcaaacagc ttcgacagaa
     4441 ataggagtta attaaaaata atgaccgagc tagctctaac tgtgggatgt gataacgagt
     4501 cttgctatat ggtgtttcag cttcgtaaat aaatgaactc tgcgctccat ccttgttgcg
     4561 tacggaagca aaggacgaga gattaggaaa atcgattatt ttggctcgtt gaagatccga
     4621 tgcttcaaac tcaatcacac gatccgcctt atttatttgc tttgttgtac tgttaaccac
     4681 cttagctcga cttaataaag gcaaatgagt aaatcgagtc catttccgtt cttgcattgg
     4741 attaaaccaa acaaaaatac gccactcctt atgacccgag ttacggaata aatgaccaat
     4801 atgtacaacc tgcacattgt cattaaatgt agctagccta accactcact cttccccata
     4861 aaccatttcc aagaatcttt gtgcttcctc agttatccgc tctttagata acgttgcact
     4921 tcttaataat cgccagcgcc taagctcaac atcttcctgt ttaagcttaa taaaagcttg
     4981 gctaatccgt ctaatttgat aatcttccac actctctgag taacgcttta agcaaagcgc
     5041 taccaaaggc agtttctgta gattttttgc aagagaggtt ccgttaggag tttgctttaa
     5101 cagccagctc gatgtcgctc ttgggtgatc aaggctacta tctagacgct ttatgattct
     5161 taatagttgt cgtacagcaa ttcgatctct ttggttccaa tcaactctag gggcgggtgc
     5221 cagacgctct tgttgatgct gttgattcca gtgaactagc caatccctgt catgtcggta
     5281 aagccaagcg tatagcaccc caccctctaa agactgtctt gccgccttaa ttccttggta
     5341 tttatgcact agttgctgcc agtctttatg cttaacagat aaatcttcag agttaggttg
     5401 cacagactgg ctaacaggtc ttgtcgttat agagtgctca gtaagagcac ttgcctgctg
     5461 tagcgcttct ataaccgtta gttttggcaa taaggcttgc cacacaatac tatgctgtaa
     5521 ataactaaag gcttttctat gcttacggaa tatacttttc agccaacacg tgtccttgtt
     5581 ctctgccaac tttaaatcca gtttctctag tgcctcatca ctaaaagtct gcctcactct
     5641 ctccgccacc aagtcatgac gaatgtgctt gcttttggtt agccctagat cctgcgctaa
     5701 gcgctgataa aacagcgtcc actgctcaag gcttggggaa agctcttgcg ctcgtggagc
     5761 atctaacaga ggggctatat aagcagctag tgctgttaat tgagataggg agtctttggg
     5821 gtagtctgaa agcagctcag tatgacccaa agcccaaaat tgatgtcggt gatcatctac
     5881 agctctatca aagaagacta aagcaccgtg ttttggacaa tatggcaaag cgggcaaata
     5941 ccaatctcgt tgccaaaagg cttccccata cctatttagc tgaagagcaa cgcaatcagg
     6001 gcagtatcta aagcggttat cgctcttaac tctagaagca gcgactccta gcattaaatg
     6061 caccgcacct tgcgcttggt actccattaa ccgaatagct tcgtctcggc gctccttgcc
     6121 tacaaacgga gcatataaag ggaataaggt atgctcataa ataagctgct gaacagcgta
     6181 acgtcctgtt tgatgtagat gtcttgctat cacacctaaa tgcgagggca gacctaaggt
     6241 agcgaccacc ttgcggttgc catacacctc atccaacagc tgcttaggac taacaatccc
     6301 ttgataaacg cctgcccgtg caatagtgct ataaatcagc tcattcgagt acggaacagg
     6361 aaagtttctc atggctaacc tgcttgttta aataagctcg ccatatccac tattacccct
     6421 ttccctttta accgttcatg catggttttt tcaggttggc gttgtgaata gatataacgt
     6481 aaatccgtat caggcaagct atcccagtcg ctgggcttga ctacctttat agccgaaacc
     6541 tttttactct tggagggctt ttctagttct gacactaccg tttcgccttc catcaaccac
     6601 tgcaaaacaa gaggcagtaa cttttgtctt gtcatcgttg gattctggct aaacgctttt
     6661 ttaatagtgg gaattaacag gcttgaatcg taatcctctt tcagcatcag atataaatga
     6721 cgctgatctt cggtatctaa ctcttgaagg gctttttctt ctggtgtttg ttcttgtatc
     6781 gctgcgatat ctagctgaag ttggattaac cgtttatcaa tctcgggaac gactagatca
     6841 gaataacgag caatgcgttc tgggataccc gagcgtaatg cctctagcat ggggtgcaca
     6901 ggctttaact catcttgata cacttgccgc aataaaccag cggtaatacg ctcattgcct
     6961 aaagctagcg cacggagctg agcgagtaca aaaagtttta ctacaatgtc catcactcct
     7021 tggcttagct cataccacac atcacggacc tcatccgata acagcgcatc tttgcgttgt
     7081 aaaagctgta attgccaaag attatccgta aaagcgatcc actcttgatt gggctttcca
     7141 cgttgcgttt gttgtatagg atcccagaat atagctccaa accctgcccc tctacgtgca
     7201 gaccgcaaat cagcctcaaa aatctctcgt gctttagggg taccaatcaa catcactggt
     7261 acgccaataa tattcaccat cgtcacaaaa aagttcagca tctcttgaga tccacccgaa
     7321 cgagagcggc ttaaatgctg aatttcatca ataaccaaca accctaaagc atgtgcattg
     7381 gctatttgcg acatcaaagc caacatggtt tctataccat gacgttttaa gccataacga
     7441 cgctcatagt tcgagcccaa ggctcgatcc aacgctctga aaaaattcaa gcagatttct
     7501 tttagcgaac cattatgcga gcagtctatt ttcaaataca ccacctgctc tacattgagt
     7561 tcacgatggt aaatcacctg aggatacgtg gctagaatac gatgaagaga ggtcgtcttc
     7621 ccactaccag aacaaccaat taacaataag ctttgtgccg tagatcgtgc ctcctcaaag
     7681 cgaaatgtct ccaactctcc cgtttgaaca cgctcataac cattttgtaa atgcttttgt
     7741 aaatctcctg ttttaggatt tctgcctacg tagccacctc gaatcatgac cgaaatacgc
     7801 tcacttagta gcaaatgcgt acctaatggc tgaaaatagt catctggaat acgacaaatg
     7861 gtatgagctc tgataacacg ggacttttgc aagtcagagg aagtaagctg taaagaggat
     7921 ttcagtgatg cagcactatt cactgactct tgtaatggtg gtaaggcctc gataaaagga
     7981 ttatcacgat aagcctctac ccccgtatca cgataaactg cttgaatccg ggtagcactc
     8041 atgactcatc cttttctggt ggatcctgaa ataattcagg cacgtatgtg ggtaggctgt
     8101 aatcttcttg atcatccgct tccactgcgt tgaaaggaat aactttagcc tcatcacctg
     8161 aagagcttgg cttcaaatgc tccgcacgtt ttttacgctc cgaggtcacg gcttcttttt
     8221 tattagtttt aatctgctta atgcgtgttg atttgggctc agtagtactg ggcgttaact
     8281 tattcgcttt ctgaatggtt tgctgaataa acgcctcaag ctccctgcgt ttagttaact
     8341 catcctgctt cgcattggct ttattgtgtt tttcttgtgc ttgtatatcc caaacctccc
     8401 aaaatgagag acctttaaac tgccgactac gttccgtcag attacagcgc caaaatacac
     8461 ggctgccaac ttgcggaaac aaataaatcg tatcaaccag cactgggtca taagccgctt
     8521 ctaaatgttg aggtctagct atatcagtgc tccgctgcaa ccaaccctca cgcagaatct
     8581 ctgaccccga gtaatacaaa ccccacaaat taacgccaaa tgaagaaata gagacctttc
     8641 ggcgaggcag taacgctact cgcaactgct cttgctccac agcccttaaa ctacctgtac
     8701 gatgctgcat accccattgc catagctgga caggaataga cggtaaatct gtaggaaaat
     8761 cagcatctcg atcgtattta tccatcacca gatggttatt tctgaataag atcgtacgca
     8821 aaataatttg tgtgaactca aataccgaca gagatgcatc taacctatag tctgtttcac
     8881 catggctttt gatccgactg ccctctacaa tgccaggtgc aaaggactta aactcggctt
     8941 gtagtgttct aaaagtgctt tccactatgc ctttagcatc gccacgtcta ggtggagcac
     9001 tttccactcg cacattaaaa ctagaaacta aggcttcgac ctgatgactc attaattcgc
     9061 cacggtccgc tagcaacaca tctggcaaac ctacacacgg ccagtctgag ctactaatct
     9121 caatatcatg ctgggcacaa atggccgttt tgtcagagca agcatttaca aaagcctgca
     9181 tcgccaccac ataagacgga ttttcaaagc cgatataaaa gcccgtgatc atccgactaa
     9241 acacatcaat cacaatgtaa agcgttggtc ttcctatgat tttttggcga tcatgatgat
     9301 ccactaaata aatatcagca atcgtggcat caatctcata acgactccca gggcctaacg
     9361 cctgagaagt ggctgtacta cttaagggtc gtacgtcttt tttatatacc cctgctttaa
     9421 ctctagactt taagcgctga gctttagggt attctcgatc ataaaaataa cgaaactgac
     9481 gtagtgttgg gtaatcctct tggggaatgc gaggaaaata ctgagcaaac aagtccacaa
     9541 atcgtctata ggcaacggtg gtctttgtac ctttttgatt taacaggtgc ttttctatgg
     9601 tcaacctaaa aaggcgttca atctcgggcg ttaccttggt tccttcaccc ttaccatatt
     9661 ctctggctcg gccaatcttt gctgttcctg tcgctgaacg tctttcccct ggtgcaccgc
     9721 tgtttttgta gtcaggaatt aatgcattag gcgtttgacc acgctgccag taacggcgta
     9781 acaacttata aactgtagcc ttagtaacct tatgttcttg gaccacatgc tcaacgagtt
     9841 cgcttctgac tttagggtcg aaacgatcct tactattaat aataggaaga atttttcgat
     9901 aatcctcctc gcgcttctgg aagctgacag aatctacaga cggctcttct aaatcaagat
     9961 gtacataagg gtcatcagtt ctaactaagc ggccttcatc taaatactgc attagcaaca
    10021 actcagccct agcttgtgga actcctttat cagcgcttat ttgcatccag acaacttggc
    10081 catcctctat agccaaaatg cgatacggat cattatcaaa tagcacaacc tcattaattt
    10141 gccacatagc gcaactcctc catattcact acttggctaa tacagagatc tgcacactta
    10201 tttgccctga aagacttata aatattgaac ttaataaaac catttgcggt taaggctcgt
    10261 atctcactca atgttttgcc taactccaaa tcataagcaa tatcaacctg cttacagaca
    10321 ttgataatgt tttcatctcc tttttcttgc aggatatggg ccaatgggga tagttgtgct
    10381 aaaagctccg cagaaacttc ttctgttttc actgaataaa gccattcaat attttctttt
    10441 actacgggat ttatttcttt atcagtaaaa atgaaccaag gaatttgctt ttgctgccaa
    10501 tagcgacgct ctagttctag tttttctaag gtacgctcgt cttgtaaggc tgctgcaggt
    10561 ttgacttgaa tagcaaactg ctcaaaagga ccatctttgc agtccactaa aaaatcagta
    10621 gacataacct gatctacacc acgaataaca ggatgcttaa taccactatc tattgcaatc
    10681 tgcctggtat cactaggtaa taaggggaac tgctcgcgta tatctagcac gctgctctcc
    10741 cactcaagac tgagaaaaac agcaagctct aagtcagata gcaaatgatg gactcgtccc
    10801 gtcttatgag aataaatacg gtgggaacga cctgaagaag gaacttcttg tactgttagc
    10861 catggaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg acgggcaatt
    10921 tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta tgctttttca
    10981 cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga ctttattgtc
    11041 atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacaa gccctttttt
    11101 atctatgata gtgattactc aaccgtaacc gattttgcca ggttacgcgg ctggtcaacg
    11161 tcggtgcctt tgatcagcgc gacatggtaa gccagcagct gcagcggaac ggtgtagaag
    11221 atcggtgcaa tcacctcttc cacatgcggc atctcgatga tgtgcatgtt atcgctactt
    11281 acaaaacccg catcctgatc ggcgaagaca tacaattggc cgccacgcgc gcgaacttct
    11341 tcaatgttgg atttcagttt ttccagcaat tcgttgttcg gtgcaacgac gataaccggc
    11401 atatcggcat caataagcgc cagcggaccg tgtttcagtt caccagcagc gtaggcttca
    11461 gcgtgaatgt aggagatctc tttcagcttc aatgcgcctt ccagtgcgat tgggtactga
    11521 tcgccacggc ccaggaacag tgcgtgatgt ttgtcagaga aatcttctgc cagagcttca
    11581 atgcgtttgt cctgagatag catctgctca atacggctcg gcagcgcctg cagaccatgc
    11641 acaatgtcat gttcaatgga ggcatccaga cctttcaggc gagacagctt cgccaccagc
    11701 atcaacagca cagttaactg agtggtgaat gctttagtgg acgccacgcc gatttccgta
    11761 cccgcgttgg tcatcagcgc cagatcggat tcgcgcacca gagaagaacc cggaacgtta
    11821 cagattgcca gcgaaccaag gtaacccagc tctttcgaca gacgcaggcc cgccagggta
    11881 tccgcggttt cgccagactg tgacaaggtg atcatcaggc tgttacgacg cacggcagat
    11941 ttacgatagc ggaattcaga ggcgatttcg acgtcgcacg gaatacctgc cagcgattca
    12001 aaccagtagc gggaaaccat accggagtta taagaagtac cacaggcgag gatctgaata
    12061 tgctcaacct tcgacagcag ttcgtcggcg ttcggtccca gctcgcttaa atcaacctga
    12121 ccgtggctga tgcgcccggt aagggtgttt tttatcgcgt tcggctgttc gtagatctct
    12181 ttctgcatgt agtgacggta aataccttta tcgcccgcgt catattgcag attggattcg
    12241 atatcctgac gttttacttc cgcgccagtt ttatcgaaga tgtttaccga acggcgagtg
    12301 atttccgcaa tatcgccctc ttcaaggaag ataaagcgac gggtcaccgg caacagcgcc
    12361 agctggtcag aagcgataaa gttttcgccc atacccaggc caatcaccag cggactacca
    12421 gaacgtgccg ccagcagggt atccgggtga cgggagtcca tgatcactgt accgtacgca
    12481 ccgcgcagct gcgggatagc acgcagaaca gcctcacgca gagtcccgcc ttgtttcagc
    12541 tcccagttca ccagatgggc aatcacttcg gtgtcggttt cagaaacgaa ggtatagcca
    12601 cgcgctttta actcttcacg cagcggttca tggttttcga tgatgccgtt atgcaccacc
    12661 acaatgtgtt cagaaacatg cggatgcgcg ttagcttctg aaggttcacc gtgggtcgcc
    12721 cagcgagtgt gagcaatacc ggtgccgcca tccagaggat gttcttccgc tgcctgagcc
    12781 agcatctgga ctttaccgag gcgacgcagg cgggtcatat gaccttccgc atcaacaacg
    12841 gccagaccgg cagagtcata tccgcggtat tccagacgac gtaaaccttc aagaaggatt
    12901 tctgctacat cacgttgcgc gatcgcgcca acaattccac acatagtttt tgattccgat
    12961 ttatatcgtt gtcggtcaac ctgtatgccc gttttttcgg gcgccccgag ccttgtagag
    13021 agtggggtta tttttatagt tactgcttgt gggagggaga ttatgttatc tcctcatccc
    13081 agatgaccgg attacccggc cagaattact ttttctttac cggacgacgc cagccttctt
    13141 tctgagtctg cggcacacgg ctgatagcta atgcattttc gccgacatta cgcgtcacag
    13201 ttgtacccgc agcaatggtc gcgcctttgc ctactgttac cggggccacc agctgagtgt
    13261 cggaaccgac aaacacatcg tcgccgataa tggtcttaaa tttattcgca ccatcgtagt
    13321 tgcaggtaat ggttcccgcg ccgatgttaa cgttatcgcc aatttccgca tcgcccaggt
    13381 aagtcagatg cccgactttc gagcctttac ccagacgcgc ttttttcatc tcaacgaagt
    13441 taccaacgtg tgcaccttcc agcaactcag caccaggacg caaacgagca aacgggccaa
    13501 tagtacaggc cgctgccaga ttcgcgtctt ccacaacggt atacggactg atttcgcaat
    13561 catcgccaat cacgctgttt ttaatcacgc aaccggtgcc gattttcacg cgatgaccga
    13621 gagtcacgtt gccctcgatg ataacgttag tatcaatttc aacatcgcgc ccgtgagtaa
    13681 gcgtaccgcg cagatcaaaa cgcgccggat cgcgcagcat aacgcctgct aacagcagtt
    13741 tttcagcctg ttcggactgg taaacgcgct ccagacggga gagttgcagg cggttattca
    13801 caccttctac ttcgcttaaa cgttgcggat gaacggcgac gatttcacgc ccttcctgat
    13861 acgccagcgc aataatgtcg gtgatgtagt attcgccctg agcattattg ttggtcagct
    13921 tcgccagcca gcgtttcata tctgcgccgt tagcgatcag aatgccggtg ttgatctcct
    13981 gaatctgacg ctgctcgtcg gtggcatctt tgtgctcaac aatgccggta actttgccat
    14041 tttcacgggt gatacgtcca taaccggtcg gatcatccag tttcaccgtc agcagaccaa
    14101 tgccaccctg cggtttagca tcacgcaggc gctggagtgt ttcgacagag atcagcggca
    14161 cgtcgccgta gagcattaaa atgtcttcat catcggcaaa gaaaggtgcg gcctgctgca
    14221 ttgcatgacc cgtacccagc tgctctgcct gaagcaccca gttaaggttg tcgtctttca
    14281 gcgcctgttt aagcagatcg ccgccgtgac cgtacaccaa gtgaacgtgc gctgcgccta
    14341 attcattcgc agcatcaatg acatgctgaa ccatcgcttt cccggcaagg gtatgcagca
    14401 ctttcggaag atcggaatac atgcgcgtgc ctttgcctgc ggcaaggatc actacgctca
    14461 tagcattatt caacatacgc gtcctgactg taatttgaga acgaatttaa accgcttcac
    14521 cttgaaaaaa ctacattttt ttcatcgtga aatggacaga ggataaattg ttcaataacg
    14581 gattatcccc gctgacgaaa gtgccatttt cgaccatcgt cacgcctttt gtctgcagaa
    14641 aattaaggca gtaccttcat ttaagcgtca ggtggatgtt tttgctctta tttcgatcaa
    14701 tgaataaacg ggaaaacagg ggtgttttat atcagcagga tctatgtgaa cgctcttcag
    14761 gacggatcac gcgtaaaaaa aagccagcct gtttccagac tggcttttgt gcttttcaag
    14821 ccggtgttac atcgcttttt tggtcaactc gataacgcgc agctgcgcga tcgctttggc
    14881 cagttccgca gacgcctgag cgtaatctac gtcgccgtga gagctgctaa tgtgctcttc
    14941 agccttacgt ttcgcttcca tggctcgcgc ttcgtcgaga tcctggccgc gaattgcggt
    15001 gtcggccaga acggtcacgt tgccaggctg cacttcaaga atgccgccag acagatagat
    15061 aaactcttcg tgaccgtgct gtttcacgat gcgaatcata ccaggcttaa tggcggtgag
    15121 cagcggtgcg tggccagggt agatccccag ttcaccttcg ctacccgtta cctggatttt
    15181 ctcgaccaga ccagagaaca tttgttgctc tgcgctgacg acgtccaggt ggtaagtcat
    15241 tgccatatca ccctccgatt aaggcgttaa agttttttgg ctttttccac agcttcttcg
    15301 atggaaccga ccatgtagaa cgcctgctcc ggcaggtgat cgtattcgcc ttccatgatg
    15361 cctttaaagc cacggatggt gtctttcagg gagacgtatt tacccggaga accggtgaat
    15421 acttctgcca cgaagaacgg ctgggacagg aagcgctgga tcttacgagc acgcgctacc
    15481 accagtttgt cttcttcaga cagttcatcc atacccaaga tggcgatgat gtctttcagt
    15541 tcctgataac gttgcaggat ggactgaacg ccacgcgcgg tgtcgtagtg ttcctgacca
    15601 accaccagcg ggtccagctg acggctggtg gagtccagcg ggtcaacggc cgggtagata
    15661 cccagagacg cgatctgacg gctcagtacc acggttgcgt caaggtgcgc aaaggtggtt
    15721 gccggagacg ggtcagtcaa gtcatccgca ggtacgtata ccgcctgtac ggaggtgata
    15781 gaaccagttt tggtggaggt gatacgttcc tgcagaacgc ccatctcttc cgccagggtc
    15841 ggctgataac ctaccgctga aggcatacgg cccagcagtg cggatacttc cgtaccggcc
    15901 agggtgtaac gatagatgtt gtcaacgaac agcagaacgt cacgaccttc gtcacggaat
    15961 ttctcagcca tggtcagacc ggtcagagca acgcgcagac ggtttcccgg cggctcgttc
    16021 atctggccat acaccaggga tactttgtcg ataacgttgg agtcggtcat ttcgtggtag
    16081 aagtcgttac cctcacgagt acgttcacct acgcccgcaa acacagagta accggagtgc
    16141 tcgatcgcga tgttacgaat gagctccatc atgtttacgg ttttacctac acccgcacca
    16201 ccgaacagac caactttacc gcccttagcg aacggacaca tcaggtcgat aactttgata
    16261 ccggtttcca gcagttcctg agagtttgac agctcttcgt aggaaggtgc tgcgcggtga
    16321 atcgcccaac gctcttcttc accgatctcg cctttcatgt cgaccggttc acccagtacg
    16381 ttcatgatac ggcccagagt cgctttacct accgggactt cgatcgggtg ttcgaggtct
    16441 tttacatcca gaccgcgacg cagaccgtcg gaggaaccca ttgcgatggt acgcacgata
    16501 ccgccgccga gctgctgctg aacttccagc accagacgct cattaccatt ttgcacctca
    16561 agagcatcgt acacgcgcgg tacggcatcc tgagggaatt cgacgtcaac tacggcgccg
    16621 attacctgga caatctttcc agtagccatc ttaaatcctc tacgaaataa cctgtttaaa
    16681 ccgcggcggc ccccgagacg atctcggtga gttcctgagt aatgctggcc tgacgagctt
    16741 tgttgtatac caactgcagc tctttaatca ggctgccgcc attgtcggtc gcggctttca
    16801 tcgccaccat acgggcggcc tgctcgctgg ccaggttttc aaccacgccc tgataaacct
    16861 gagattcgac ataacgacgc agcagggtat ccagcaacgc cttcggatcg ggttcgtaca
    16921 ggtaatccca ggatttatgt ttcagatcat catcatctga tgccggtaac ggcagcagct
    16981 ggctgatggt cggaacctga gacatggtgt taataaattt gttgctgaca atgtaaagct
    17041 tgtccagacg gccttcgtcg taagcctgca acatcacttt taccggaccg atcagttcgg
    17101 acagggaagg gttatccccc atgccggtga cctgggcaac aacattgccg cccacggagt
    17161 tgaagaacga cacgcctttc gagccgatca ttgcgaggtc gcattgaacg cttttgtcgg
    17221 tccaggtctt catttccgcc agtagttttt tgaacaggtt aatgttcaaa ccaccgcaca
    17281 aaccacggtc ggtcgacacc accaggtagc ccacgcgttt aacgtcgcgg tcttccaggt
    17341 aagggtgctt atattccaga ttaccgtgtg caaggtgacc aatcactttg cgcatggttt
    17401 ctgcataagg acggctggcc gccatgcgat cctgcgattt acgcattttg gaagcggcga
    17461 ccatctccat cgctttagtg atcttttgcg tgttctggac gcttgcgatc ttactacgta
    17521 tctcttttgc gccggccatg agcttctcct caatgccttg cggcctgccc taaggcaagc
    17581 cgccagacgt taccaggatt gggttgcttt gaaggaatcg aggatgcctt tcagcttgcc
    17641 ttcgatttcg tcgttgtagc caccggtctg gttgatctct tgcatcaacg gagcgtgatc
    17701 acggtcgacg taagccagca gagcggcttc gaagctgcca attttcgaca gttcaacatc
    17761 cgccaggtaa ccacgttctg ctgcgaacag aaccagagac tgctgcgcaa cggacatcgg
    17821 cgcatactgt ttctgtttca gcagttcggt cactttctga ccgtggtcaa gctgcttacg
    17881 tgttgcatcg tcaaggtcgg atgcaaactg agagaacgct gccagttcac gatactgtgc
    17941 cagagcggta cggataccac cggacagttt tttcatgatc ttggtctgtg ctgcaccacc
    18001 aacacgggat acggaaatac ccgggttaac cgcaggacga ataccggcgt tgaacaggtt
    18061 ggtttccagg aagatctgac catcggtaat ggagattacg ttggtcggaa cgaacgcaga
    18121 aacgtcaccc gcctgagttt cgataatcgg cagtgcggtc agagaaccgg ttttcccttt
    18181 cacttcacct ttggtgaagg cttcaacgta ttcggcgtta acacgtgcag cacgctccag
    18241 cagacgagag tggaggtaga aaacgtcgcc cgggaatgct tcacgtcctg gcggacgacg
    18301 gagcagcagg gagatctgac ggtaagcaac agcctgttta gacaggtcat cgtaaatgat
    18361 cagcgcatct tcaccgcggt cacggaagta ttcgcccatt gcgcaaccgg catacggtgc
    18421 caggtattgc agtgcagcgg attcagacgc ggttgctacc acaacgatgg tgttagccag
    18481 tgcgccgtgc tcttccagtt tacgtaccac gttagaaatg gtggacgctt tctggccgat
    18541 agcgacatag atacatttga taccggaatc gcgctggttg atgatggcat cgatagccag
    18601 tgcggtttta cctgtctgac ggtcaccgat gatcaattca cgctgaccac gaccgattgg
    18661 gatcatggag tcaacggctt tataaccggt ctgtaccggc tgatctacgg actgacgttc
    18721 gataacgccc ggagcgattg cttctacagc agagaagccg tcgtgatcca gcggaccttt
    18781 accgtcgatt ggtgcaccca gagtgttaac cacacggccc agcaggccac ggccaaccgg
    18841 aacttccagg atacggccag tacacttaac tttcatgcct tcggcaaggt cagcgtacgg
    18901 acccataaca accgcaccta cagagtcgcg ctcaaggttc agtgcgatag cgtaacggtt
    18961 acccggcagg gagatcattt caccctgcat acaatcggcc aggccgtgaa tgcggataac
    19021 accgtcactt acagaaacaa tagtaccttc gttgtgagct tcactcacaa cattgaactg
    19081 agcaatgcgc tgcttgatca gttcgctgat ttcggtggaa ttcagttgca tgctccagtc
    19141 cccttaagac tgcaagacgt ctgcaaggcg ctcaagacga ccgcgtacgc tgccatcaat
    19201 gaccatatca cccgctcgga tgataacgcc tgccattaca gacttatcga ttttgcaatt
    19261 cagcttaact ttgcgtgaca gacgtttttc catcgcagca gaaattttcg cgagctgttg
    19321 ttcactcagt gcggcagcgg aaatgacgtc tacctcagcg gtagcctcac tcacggcacg
    19381 aaggtgaata aactgctcca gaacatccgg gagcgcgtta agacgaccat tttcagccat
    19441 tacccgaatc aggttctgac cgttttcgtc cagttgctca ccacaaactg cgataaacga
    19501 ctcggcgagc gtttctggcg caagcgcgcc agagagaagc tctgccattt gttcgttttt
    19561 ggttacctcg gcggcaaacg ccagcatgtc ctgccagcgt tctacacttt ggtgttcgac
    19621 ggcaaagtca aaagctgctt tggcgtaggg gcgagctacc gtaataaatt cagacatcag
    19681 cccctccctc cttacagttc agcgacaagt ttatccacga tgtcgctgtt agcagcttca
    19741 tccacggaac gttcgatgat cttctcggcg ccagcaacag ccaggatagc aacttgctta
    19801 cgcagctctt cacgggcacg tttacgctcg gcttcaattt ctgcctgcgc ctgggccacg
    19861 attttagtac gttcctgttc tgcctcagct ttcgcttcgt ccagaatctg cgagcggcgt
    19921 ttgttcgcct gctcgatgat tacctgggct tccgctttcg cttttttcag ctggtcggtc
    19981 gcgctggcct ttgcaaggtc aaggtcctta tgtgctcgtt ctgcggaagc aaggccgtca
    20041 gcaatttctt tttgacgttt ttcgatggct gccattaatg gcggccatac gtacttcatg
    20101 cagaacagaa cgaacaggac aaacgcgatg gcctggccga ggattgttgc gttaagattc
    20161 acagcacaat gcctctattt agttaacgtt ctgatattgc tctttaaata aaagcaacgc
    20221 ttactacgcg acagcgaaca tcacgtacag acccagacct acagcgatca tcgggatagc
    20281 atccaccaga cccataacga taaagaactg agtacgcagc agaggaatca gatcaggttg
    20341 acgcgctgcg ccttccagga atttaccccc gaggatgccg ataccgatcg cagcaccgat
    20401 tgccgccaga cccatcatca cagcggcagc catgtacagc agatccatat tcaggttttc
    20461 catgacagtc tccagtttgt ttcagttaaa acgtagtagt gttggtaaat taatgttctt
    20521 cagacgccat cgacagatag acgatcgtca gaaccatgaa gatgaaggct tgcagcgtaa
    20581 tgatcaggat gtggaaaatg gcccacggca cattcaggat ccactgtgac caccacggca
    20641 acagaccagc aatcagaatg aaaatcagct caccggcata catgttaccg aacagtcgca
    20701 aaccgagtga aactggtttg gacagcaggc ttaccccttc aaggattaag ttgacaggaa
    20761 tgaacgccca gtgattgaac ggctgcagcg tcaactcttt cgtgaagccg ccgatgcctt
    20821 tcattttgat gctgtagaac agaatcagga taaatacgcc cagtgccata gacagcgtta
    20881 cgttcacgtc cgcagacgga accacacgca gtgcaggcag acccagtaca tgttcagcaa
    20941 tgtacggcag caggtcgata ggcagtaaat ccatcaggtt catcaggaat acccagacga
    21001 agatcgtcag ggccagcgga gcaatcagct tgcttttgcc atggtacatg tctttcacgc
    21061 taccattaac aaagccgatc accagctcaa tcgcggtctg aaacttacct ggcacaccgc
    21121 tggtcgcctt tttggctacg ctacggaata aaaccaggaa caacagaccc agcaccaccg
    21181 agaagaacat ggagtcaata ttgattgtcc agaaggtggc tggggggttt tgtggatcca
    21241 ccagcgagaa tgtacgcagg tccagctgaa ggttattcag gtggtgtcct atgtaatcct
    21301 gcggcgtcat attttctgaa gccatgatgc cttttaccct ttgttgttaa ttacagccgg
    21361 tgccagtatc tgaaccacca gcaccaaaac ccacgtaacg atcagcggca agaataccgc
    21421 ctttaaaacc gccaacgcca ccaccagtaa caccaacatc gccagaactt tgaaagcttc
    21481 gccaaatgcg aatgtccagg ccacccggcc tttcgctggt gtatacgcct ggtgacgcca
    21541 ggcaaatatc ataaacaaaa cgttaggcag aaagactgcc aggcccccgc ttattgcaga
    21601 gacgccccag aaggggtctt tgaggctgaa cagcaatcca cttgctatca ccaccagtaa
    21661 ctgaacgagc agaagcttcc gagcaacgtt tcgactcacg agcgacacag acatcacgtt
    21721 tttcactcct gctcccttcg aggtatgccg cgtgtcgtat aaaactttct ttaaggctta
    21781 gagtcaagca tcaaaaagcg gtcaaattat acggtgcgcc cccgtgattt caaacaataa
    21841 gtagccaaaa ggtgaataaa tgtttaaata tttttccagt gcatacaatt gcgacttttc
    21901 cgctaaccct gttcgatcat gaaaaactgt aaataacgcg taaacactag tgataaagcg
    21961 tgcttcagat cacatattgc gcatgttcgc gcacagtata tttatttact tggcaaa
//
LOCUS       DAZOXR010000588        11014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.147, whole genome shotgun sequence.
ACCESSION   DAZOXR010000588 DAZOXR010000000
VERSION     DAZOXR010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.147"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            110..1015
                     /locus_tag="ACHUNN_004879"
     CDS             110..1015
                     /locus_tag="ACHUNN_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR0578986.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHUNN_004880"
                     /gene_synonym="mrx"
     CDS             1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACHUNN_004880"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR0578987.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHUNN_004881"
     CDS             2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACHUNN_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR0578988.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4091)
                     /locus_tag="ACHUNN_004882"
     CDS             complement(3327..4091)
                     /locus_tag="ACHUNN_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR0578989.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(4318..4623)
                     /locus_tag="ACHUNN_004883"
     CDS             complement(4318..4623)
                     /locus_tag="ACHUNN_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR0578990.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHUNN_004884"
     CDS             complement(4634..5839)
                     /gene="chrA"
                     /locus_tag="ACHUNN_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR0578991.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            complement(5995..6198)
                     /locus_tag="ACHUNN_004885"
     CDS             complement(5995..6198)
                     /locus_tag="ACHUNN_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0578992.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHUNN_004886"
     CDS             complement(6326..7165)
                     /gene="sul1"
                     /locus_tag="ACHUNN_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0578993.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(7159..7506)
                     /locus_tag="ACHUNN_004887"
     CDS             complement(7159..7506)
                     /locus_tag="ACHUNN_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0578994.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(7712..8500)
                     /locus_tag="ACHUNN_004888"
     CDS             complement(7712..8500)
                     /locus_tag="ACHUNN_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR0578995.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACHUNN_004889"
     CDS             complement(8631..9104)
                     /gene="dfrA17"
                     /locus_tag="ACHUNN_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR0578996.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            9262..10275
                     /gene="intI1"
                     /locus_tag="ACHUNN_004890"
     CDS             9262..10275
                     /gene="intI1"
                     /locus_tag="ACHUNN_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0578997.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            10478..10828
                     /locus_tag="ACHUNN_004891"
                     /pseudo
     CDS             10478..10828
                     /locus_tag="ACHUNN_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
BASE COUNT         2289 a         3248 c         3186 g         2291 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcacatcac cctggaaatg gcgccacttg
     4081 aaatccgtca tcgttccgtc cgtccaatct ccgccaagca tgctcaagct tcacgatttt
     4141 tgcaacagag cccacacgag tattgagcat agtcgagatt ggtgcagatc acttctgata
     4201 ttgaactgtc aggagctggc tgcacaacag ccattacgcc caatcaactg gtgcagtcgt
     4261 cttctgaaaa tgacaatcca gttagggtat agctcaacct gacatagaag caaaaactca
     4321 accaccttct accaactctc cgaacagctc cttgaccttt gttttcgcat cagcaagtgc
     4381 agttctgcct tgttcagtga tgtcataaac acgccgttca cgtcgcccgg tgcgttcgtg
     4441 gcgtgaggtc agatagcctt ttttttccag gccgtgcagc atcgggtaca cggtgccagc
     4501 gctcatctcg tagccgtgtc ggcgtagctc ttcgatgatc cccagcccaa agacaggttc
     4561 ctcggctgca tggtgaagga tgtgcaggcg gatcaaaccg ccgtagaggt ctttgtcagt
     4621 cattttttgt gcctcacaga gcgacgctca acagccaccc agctgcaccg ctaccgagga
     4681 caaccagcca cggcgggagc ttccagaaca taagtgcgac aagggcaact aatgccaagc
     4741 cgaagtcttg cggctgaaag atggcgctag tccatacagg ctgatacagc gcggccagca
     4801 gcaagccgac tacagcggca ttgatcccgg ccagcgcagc ttggatgcct gtattgcggc
     4861 gcaaacgctc ccaaaatggc attgatccga cgaccagcaa gaacgagggc gcgaagatag
     4921 ccagcagaca cacaatgccg ccgatccagc ccgacggggc ggtgttcatc gaggcaccaa
     4981 gaaacgcggc gaacgtgaac aaagggccgg gcaccgcttg agctgccccg taccccgcga
     5041 gaaaggattc attgttgacc cagccggagg gcaccacttc ggcttgcagt aatggcagca
     5101 caacgtgacc accgccgaac accagtgatc cgacacgata gaaggaatcc accattgcca
     5161 tggtttgact tggcatcagt tcggccaaca ccggcaggcc aatcagcaag acaaagaaca
     5221 gcgagagcca aagcacgccg gcccggtgac tgaccgtgat aggtaggggg tcatgctcaa
     5281 caactttcgc tggcttgaac aataaccggc ctgcgatgcc tgcgatagca atcacgccaa
     5341 cctgtcccca cgcggacggc acaagtaaaa cgacgcaggt agcaattgcc atgatggtga
     5401 ctcgcagccc atccgtgcat aggttacgcg ccatgcccca tactgcttga gcgaccacgg
     5461 ccacagccac cacttttaag ccatgcaacg cgccctgcga gacgtaatcg ccatagctgg
     5521 agatgccgag cgcaaaaagg atcaaggcta tggcagacgg cagcgtgaag ccagcccaag
     5581 cagccagcgc cccgctgtat ccagcccgag acagtcctac cgctatgccg acctggctgc
     5641 ttgcaggccc tggcaagaac tgacaaagcg cgaccaagtc agcatagctc cgttcggaga
     5701 gccagcgccg ccgtgtgaca aattcggcgc ggaagtagcc caagtgcgca atggggccgc
     5761 caaaagatgt caatccaagc cgcagaaaaa taagaaagac cgaccatggt ctgctgtcat
     5821 cggtagggtt attcgtcata ctttcgcctt catgatctgc aacgagttga tcaataataa
     5881 gcgaaattcg ataacgaaat tcgatataaa tctagaaaaa aatacctcta tgtgtactac
     5941 gcagttttag ctgtggcttt cacaggagca cgcttactta cggcttagcg tgctttattt
     6001 tccgttttct gaggcgatcc ctaggagctc ggatctcagg acgaaggtct ccgcgaatgt
     6061 ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg gcggacatcc acgccgcagc
     6121 gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg
     6181 aggctcctcg ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag
     6241 tttgatcctg acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg
     6301 ctagcgggcg gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc
     6361 gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg
     6421 cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg
     6481 ctggacccag atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca
     6541 ccgagaccaa tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt
     6601 gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc
     6661 ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa
     6721 tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct
     6781 gcgctgagtg cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag
     6841 ggtcaggaaa tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct
     6901 gggtttccgg ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta
     6961 agagcggcgc aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc
     7021 tggcggccgg tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg
     7081 tgacagcgcc ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat
     7141 tcagaatgcc gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga
     7201 tcgggcgagc aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag
     7261 cttttgccca tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca
     7321 gactgcataa gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc
     7381 gatgccataa ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcgctaga
     7441 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
     7501 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     7561 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcata
     7621 gttgagcggc gggcgcagcc cgtccgcttg aacgccgagt taggcatcag atgccctcgg
     7681 cgcgggtcga tgcacttttc gcacatgccg ctcaacgcaa gattctctca atcgttgctt
     7741 tggcatatcg aacgaacgcg gccgtctctt cgacgcgcat tgctaggtcg tcgtcctcgc
     7801 tacccaggta cgccgcgcgt gccttgcaga tgaggggccg atgctcggca ggcaaacgct
     7861 ccgataccca tgcggcagca acgtccttag gagcaatgag accagttgaa gcgctgtacc
     7921 aaatgcgagc aagagcaaga acgacgttcc gctcgtcacc cttccaatcc gactctgcat
     7981 tccactgggc aatagtgtcg aaaagcgcct tggagaaatg ctccttcggc accggctcga
     8041 aaaacgtggc tgcggatggg cctagaagcg caaggctgtg ttgcctcgcc ttggtcagca
     8101 aaatcgcaag atcgtgatcc agaacggcag gctcgaacgt tccggaaagg atgtcgtggc
     8161 ggagccactc accgaactga agctcacgcc gcgccggata gcgccaaggc actacttcgc
     8221 ttcgagcgac aacagttagc tccagcggtc gccatgttcc gccatcgcct ggcggtgatg
     8281 agactttcag caaatcgagc attagcgcct gccggagcga atcgttaggt gcggcgctga
     8341 cggtcacgag caagtctatg tcgctgtccg gcttcagccc tccatcgatc gcagatccga
     8401 acaggtggat tgtgtccagt gtcgcagcca gatggcgctc gatcaccgcg cgagcgtggg
     8461 acagctgctt gaaaacttgt gcagggaaaa attcacccat gatgcctaac gttaagttca
     8521 gcggcagctt ttaagttgcg gctttgtgga atacttttgc gcagcaaaac cacaaagacg
     8581 cgacttaaaa gctgtccaag gagcgaagcg actggtgctg caacgcattg ttagcctttt
     8641 ttccaaatct ggtatgtata atttatatta gacataaaaa actgttcaaa aaccaaattg
     8701 aaattctcag gcattatagg gaatttgata tcaccttcga cttcaacgtg aacagtagac
     8761 aaatgaatta tatctgcttt ttcaataagg ctattataga tttgaccccc gccagagaca
     8821 tatacatgat ctgtaacttt tgatagctct ttcaaagcat tttctattga aggaaaaact
     8881 aggacgtttt catttgagct tgaaattccg ttctttgaca ctactgcata tttgcgattt
     8941 ggaagaacac ccatagagtc aaatgttttt cttccgacaa ggagccattg attatatgtg
     9001 agcgctttaa agagtagttg ctcacctttt actgaccacg ggatatcagg accactaccg
     9061 attacgccat tttctgacac tgcagaaatc aatgatattt tcaatttaac tcccttaatg
     9121 gctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     9181 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     9241 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     9301 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     9361 gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     9421 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     9481 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     9541 ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg
     9601 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     9661 cgtttgttcg cccagcttct gtatggaacg ggcatgcgga tcagtgaggg tttgcaactg
     9721 cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg caagggctcc
     9781 aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga gcagctgtcg
     9841 cgtgcacggg catggtggct gaaggaccag gccgagggcc gcagcggcgt tgcgcttccc
     9901 gacgcccttg agcggaagta tccgcgcgcc gggcattcct ggccgtggtt ctgggttttt
     9961 gcgcagcaca cgcattcgac cgatccacgg agcggtgtcg tgcgtcgcca tcacatgtat
    10021 gaccagacct ttcagcgcgc cttcaaacgt gccgtagaac aagcaggcat cacgaagccc
    10081 gccacaccgc acaccctccg ccactcgttc gcgacggcct tgctccgcag cggttacgac
    10141 attcgaaccg tgcaggatct gctcggccat tccgacgtct ctacgacgat gatttacacg
    10201 catgtgctga aagttggcgg tgccggagtg cgctcaccgc ttgatgcgct gccgcccctc
    10261 actagtgaga ggtagggcag cgcaagtcaa tcctggcgga ttcactaccc ctgcgcgaag
    10321 gccatcggtg ccgcatcgaa cggccggttg cggaaagtcc tccctgcgtc cgctgatggc
    10381 cggcagcagc ccgtcgttgc ctgatggatc caacccctcc gctgctatag tgcagtcggc
    10441 ttctgacgtt cagtgcagcc gtcttctgaa aacgacaatg gaggtggtag ccgagggtgt
    10501 ggaaacaccc gactgccttg cgtggttgcg gcaggcgggt tgcgacacgg tgcagggttt
    10561 cctgttcgcc aggccgatgc cggcggcggc cttcgtcggc ttcgtcaacc aatggaggaa
    10621 caccaccatg aacgccaatg aaccgagcac cagttgctgc gtgtgctgca aggaaatccc
    10681 gctcgatgcc gccttcacgc cggaaggggc cgagtacgtg gagcatttct gcgggctgga
    10741 gtgctatcag cgcttccagg cgcgggccag cactgcgacc gaaaccagcg tcaaaccgga
    10801 cgcttgtgat tcgccgccgt caggttgagg cataccctaa cctgatgtca gatgccatgt
    10861 gtaaattgcg tcaggatagg attgaatttt gaatttattg acatatctcg ttgaaggtca
    10921 tagagtcttc cctgacattt tgcagggaat tccatgactg gacagcgcat tgggggcact
    10981 gttgcaaagt tagcgatgag gcagcctttt gtct
//
LOCUS       DAZOXR010000589        10215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.157, whole genome shotgun sequence.
ACCESSION   DAZOXR010000589 DAZOXR010000000
VERSION     DAZOXR010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.157"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHUNN_004892"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHUNN_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHUNN_004893"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHUNN_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0578998.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUNN_004894"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUNN_004894"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0578999.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUNN_004895"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUNN_004895"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0579000.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUNN_004896"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUNN_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0579001.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHUNN_004897"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHUNN_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0579002.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHUNN_004898"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHUNN_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0579003.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6673..7776
                     /gene="proB"
                     /locus_tag="ACHUNN_004899"
     CDS             6673..7776
                     /gene="proB"
                     /locus_tag="ACHUNN_004899"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0579004.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7788..9041
                     /gene="proA"
                     /locus_tag="ACHUNN_004900"
     CDS             7788..9041
                     /gene="proA"
                     /locus_tag="ACHUNN_004900"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0579005.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9156..9231
                     /locus_tag="ACHUNN_004901"
     tRNA            9156..9231
                     /locus_tag="ACHUNN_004901"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Thr,seq:cgt)
     gene            9837..9995
                     /locus_tag="ACHUNN_004902"
     CDS             9837..9995
                     /locus_tag="ACHUNN_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0579006.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2539 a         2465 c         2650 g         2561 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatagctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg taacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     7681 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7741 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
     7801 tgggcattgc tgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7861 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7921 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     7981 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8041 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc
     8101 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8161 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8221 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     8281 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     8341 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     8401 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     8461 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     8521 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     8581 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     8641 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     8701 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     8761 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8821 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8881 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     8941 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9001 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     9061 aagtagccat ttgattcata aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9121 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9181 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attaagaaaa
     9241 ttaataactt agcttaactt ctctaagaaa ttgtatcttc tgttatcact tacaaacgcc
     9301 tatacgttgt tatgggcttt ggtagtgtaa cacgtatggg cgagtagttc ccaggctcaa
     9361 cctcgtaaca agttcctttg aagctggaag acgtgccacc gtctctaaac cttctaccaa
     9421 aactcttagc aagatgttcc acacctggcg cggtgtccaa tgatgctaca cgtcgtgaga
     9481 cgttgcagag gataaacagg ctaaatgatg ggaagagttt taagtgcaat agtggattaa
     9541 agcccacctc ttcaaaacct aacttgatcg ggaaagaatc aagctctagc acggtttcgt
     9601 ccaccggagt acaacgggtg cctgataaga gaagaactgc taagacttca tagcttttgt
     9661 gaaaaagccc tttttagtgg atacgcctac ggcgcttatt ttttggtata accatcaaag
     9721 gcgctttatg gctcctattg tcttgaaaat gtatctttac ttgtattgtg ttttatgcgt
     9781 agtctccctg tgtagtatgt aacttgttaa ttttcatatg aatacagagg cacacgatga
     9841 aaaacattgc agcaatcaaa cgtaataacc gcaagattca cgctcgtaag ttcctgtcta
     9901 cgccagaagg aaaagcctgg ctagagcgta aacagagaga gaacgaagaa agaaaactcc
     9961 ttagtgagtt gaaatggctt agagaggatt tctgaaaatc tccagaacgc aacaggacgc
    10021 attctaacgc tgttttcatt actcggtata gggataggtg atgtgtgttt aatgcgtctg
    10081 tgcgcttgtt ttgtgcctta ttttgcattt tcataatgcg gaagagaaca cggaaaacct
    10141 taaaattaat tattggggta tttaaggcta ttttcaggtg atttaagggt attaaacttg
    10201 atttaattga ttaaa
//
LOCUS       DAZOXR010000590         9620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.166, whole genome shotgun sequence.
ACCESSION   DAZOXR010000590 DAZOXR010000000
VERSION     DAZOXR010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.166"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..287
                     /locus_tag="ACHUNN_004903"
     CDS             <1..287
                     /locus_tag="ACHUNN_004903"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HFR0579007.1"
                     /translation="NRGYLTKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDL
                     LLAYKMGVKTLYYHNTRDGAGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            393..1535
                     /gene="nrdB"
                     /locus_tag="ACHUNN_004904"
     CDS             393..1535
                     /gene="nrdB"
                     /locus_tag="ACHUNN_004904"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0579008.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWAEYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            1613..2482
                     /locus_tag="ACHUNN_004905"
     CDS             1613..2482
                     /locus_tag="ACHUNN_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFR0579009.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            2650..3753
                     /locus_tag="ACHUNN_004906"
     CDS             2650..3753
                     /locus_tag="ACHUNN_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0579010.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQTDDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            3755..4168
                     /locus_tag="ACHUNN_004907"
     CDS             3755..4168
                     /locus_tag="ACHUNN_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0579011.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            4165..4641
                     /locus_tag="ACHUNN_004908"
     CDS             4165..4641
                     /locus_tag="ACHUNN_004908"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0579012.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            4641..5285
                     /locus_tag="ACHUNN_004909"
     CDS             4641..5285
                     /locus_tag="ACHUNN_004909"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0579013.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            5347..5766
                     /locus_tag="ACHUNN_004910"
     CDS             5347..5766
                     /locus_tag="ACHUNN_004910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0579014.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            5776..6333
                     /locus_tag="ACHUNN_004911"
     CDS             5776..6333
                     /locus_tag="ACHUNN_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR0579015.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            6494..7303
                     /locus_tag="ACHUNN_004912"
     CDS             6494..7303
                     /locus_tag="ACHUNN_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0579016.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            7486..8079
                     /locus_tag="ACHUNN_004913"
     CDS             7486..8079
                     /locus_tag="ACHUNN_004913"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR0579017.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            8267..8497
                     /locus_tag="ACHUNN_004914"
     CDS             8267..8497
                     /locus_tag="ACHUNN_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0579018.1"
                     /translation="MSYPTNVVALVDSDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            9079..>9620
                     /gene="rnhA"
                     /locus_tag="ACHUNN_004915"
     CDS             9079..>9620
                     /gene="rnhA"
                     /locus_tag="ACHUNN_004915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0579019.1"
                     /translation="MTTKTPARPQAKTRKKDKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSA"
BASE COUNT         2668 a         2032 c         2340 g         2580 t
ORIGIN      
        1 acaaccgcgg ctatctgaca aaggtggcga tcatccagaa gttctttgac caggctattt
       61 cggccaatac caactatgac ccttctcgct ttgaaggcga taaagtccca atgatgacgc
      121 tactgtcaga tttgcttctc gcctacaaga tgggagttaa aacgctttac taccacaaca
      181 ccagagatgg ggcaggaaag cgtgatgacg acgaaccgca gaatccactg acgcaagctg
      241 tagccgtcga gccagaagat gagtgcgacg gagcctgcaa aatctgacat atggtggggt
      301 gtatccccac cttctctttg atttgtaagc cttgtttaaa caaataggat aacaacttgt
      361 ttaaacacat caaaaagcaa aaggaaaaac acatgtcata ttcaacgttc cgtttgggtg
      421 ctaatgatgc aaccaaagag cctatgttcc tcggacaatc tgtcaacgtg gcacgttacg
      481 atcagcaaaa ataccgtgat tttgaaaagt tgattgaacg tcaattgtct ttcttctggc
      541 ggccggaaga agttgatatt tcgagcgatc gtatcgactt caacacgaag ctgcgggacc
      601 acgaacgtca catttttctg agcaatctcc gttatcaaac gttactcgat tcagttcagg
      661 gacgtagccc aaatgcaacg ctgctgccgc ttatctctat tcctgaactg gaaacgtggg
      721 ttgaaacatg gtctttctct gagactatcc atagccgcag ctacacccac attattcgtg
      781 gcatggtgga cgatccgagc attgtttttg acggtattgt tacggatgaa gaaatcatca
      841 accgagcgat cagtatctct gctgaatatg acaggcttta tgggatgacc tgcgagcgcc
      901 agtcgttagg cgagaaggaa tttgagcgtc tgtacgtaaa tgaatatggc tgggagccat
      961 atcctttgca ccgtcagctt ttccgcacgt tggtgtccat taatgcgctt gaggcgatcc
     1021 gtttctatgt aagttttgca tgtacgtttg cctttggcga acggaagttg cttgagggta
     1081 acaccaaaat tatgcgcttt attgcccgtg atgaagctct gcattgcgaa ggaactgaac
     1141 gcatgatccg cttcatgcgt accggtcgcg aaggtttatt gtggaaagag attgctgctg
     1201 atgaagaaaa cgtcatttac gacaccatga aatcagtcgc cgaacaagaa atgaactggg
     1261 ccgagtatct cttcaaagac ggttcgatga ttggtttaaa cgcggatatt ttgaagacct
     1321 atgtaaaata ccgcaccaat ctggctatga atcgtcttgg cctgaaggct ttatttccag
     1381 aggttaccac cgatccgctg gtctggatga acaagtggtt gttaaccgac acactgcaaa
     1441 ttgcaccaca agaggcagag caaagcacat atctggtagg tcagatcgat tctaccgtgg
     1501 acaaggcttc tctaagccag tttgcagacc tgtgaaccga tacaaagcat tatgtaacct
     1561 ggcaacgctg ggccacaatg gatcacaaga attaagaagg aacaaaacta gcatgaactt
     1621 taccaaactg actgaccacc tgaaacttgc caccgatcgt ctcattggat ttaagccaga
     1681 accatatgag ttgcatgaag gtcatggtgt agctactgaa agtatttaca agatggtcga
     1741 tcagtttcat gaactcttcc agcatccgag acgcgttatg ccgacaccag agctgcttcg
     1801 tctccgtgca agcctgattc atgaagaagc tgtagtggaa ggtattccag ctgcaatgaa
     1861 tggggatatt gagcaactgc tggatgcaat ggccgacttt ttatacgttg gtgttggtac
     1921 gatggtcgcc atcaaaggtg gtatttctac cggcatgacc tattacacgc aggaacagag
     1981 cattgatcgc tttatgcaga caatttttgt gcccggtaac actgttttcg atgatatggc
     2041 aatgccattt caggaagctc gtgaggcgtc atgtatgctc gaagagctgg cagataaact
     2101 tgagaacaag actgttaagg attctgagct gattcaggaa ctgcgccgtg tcatgaacaa
     2161 aatctatgtg gcgtgcatga tgacctatcg actggctgat ttcctcggta tcaatgtagt
     2221 cgagctggtt ggcgaaattc atcggtctaa catgacaaaa ttatggcctg ctgatgtaga
     2281 ggaacgtcgc caggctgtgg ccaactgcaa atacgactct tcagacctgg gatttcgcca
     2341 tgctgatggc accgataaga tgatcggttt tcgaatttcc gatggaaaga ttctgaagtc
     2401 tccaacctat agtgatgtcg atttatcctc ctttgttgag caagctaaag cctcagcaat
     2461 gtacggaatg atcaaaaaat aattgtaggt gattatctac taatgtatat ttgatttgcg
     2521 tataaaactc tggaacgact attctttttg gtggcctatg gccaccattt tttatctatc
     2581 tggtcttgtt ttatcaatga atgtaaactc acgtgataaa taattgatta cttatcttcg
     2641 tgaggtttta tgtcactcct tttgaatcgt gagcatacga acggtcaggt aacaaacgca
     2701 tcgtatgcaa aagttattga gacggtgctt aaaagcggcg tgcagactga tgatcgcaca
     2761 ggcactggta ctttaagcac ctgctacgtt ccctcttact acatgcttac tggtgggact
     2821 gtgccgctta tttctggaaa ggcggtaaat cttaagccac tgcttgtcga acttgagtgg
     2881 tatctgaaag gcacgggcaa catccaattt cttaaggata acggcgttaa gatttgggat
     2941 gcatgggccg atgagaatgg cgatttgggg ccggtttacg gtaagcagtg gcgtcgatgg
     3001 gaagataccc gcatcgtgag ccatagtgaa tatctgagca agatcgctac tttccgtgaa
     3061 cgcgggtaca aagtcgaggg atacctgggt atcagtgaag atcgcgtagt gctgtcccgt
     3121 gaaatcgatc agctacagcg tattgtcgac acactgcgca cgaaccctac cgatcgtcgc
     3181 atcatgctta acgcatggaa cgtaggcgag cttgaggata tgaaactgcc accttgccac
     3241 tttgtcttct ctttgtggag tcgtgagctt gattttgaaa cccgtttaac gatggcaact
     3301 gacattggtc ttcaacacag tcgcttcggt tatgagtcta tctacaccaa gatgctatac
     3361 gatctggaga tggacggcag tgttactgaa actgaactgg atgaacttgg aatccccaaa
     3421 cgcatcctca actcctgcct cgtacagcgt agcgtagaca cttttgttgg tatgccattc
     3481 aatattgctg gctatggcat tctcactcat tttctcgcga agattacggg tcacatggcc
     3541 ggtgcatttg tgcattttgg ctttgacgtg catttgtaca acaaccacat ggaaggtgtg
     3601 tgtgagctaa tgaaacgaca ggctccagag cattcagatc cggtcgttat tttccctcat
     3661 gaatggtctg agctggatga tttcaaatgg gacgatattc tgattcttgg ctatgaccct
     3721 ctaccgtgga tcaaggttcc agtggcggtg tgatatggca agaggtatgt atgtcttatg
     3781 cgaaattgaa ggtgtgctgg caaataccag ccatcgtaaa tctgtacctg acacggatgc
     3841 aggccagctc attgccggtg atgaactcat tttccccacc agccgtatgt tgcgtggttt
     3901 tgctcgctca ggggctgaag tggtgcttat cagtagccgc tctgaaactc ttgaagcgcc
     3961 cactaaacga tggctgaaag attttggcgt tgattacgac tggcttcatc tcgtaccgaa
     4021 tggcaccagt tatgagaagc atattaagcg cacattagcg gagcataaag gcgatctgct
     4081 tatcgctgcg ctggtgcacg atcctcgact ccgtgccgct ttagctgact ctcatcatag
     4141 accggtcatc tatgaggtga gcaaatgaag atgatagctg ctgttggccg taactatgag
     4201 atcggcatag cgaacgaact gccctggcgt tgttctaccg atctgaagct atttaagaga
     4261 ctcaccaaaa acgccactgt cgttatgggg cgtaaaacga tggaaagtct caaacgccct
     4321 cttccagagc gtcataacct cgttttgacg cgctctcatg gctttgtacc aaatggattc
     4381 taccctgctg gtgtggatga tgtgttgcga ttaccagagc ctgtgtgggt gattggcggg
     4441 gaacaaattt actcgctatt catgccgcat gttgaagaga tttggctctc ccacatcggc
     4501 gttgatgtgc caaacgccga tgcattcttc ccggcaagca tgatgcgtaa tttaggcttt
     4561 gtgcctgttg aaacagtttt tacccaacga gccagcgagg aagagcctgg cttttcgcag
     4621 atcgtataca gaaggtcgta atggattacc ggattgggat cactggtgct cagggcagtg
     4681 ggaaaacaac cctggcaaaa tatatcgaca aacattacgg aatcccttac gtggatgctg
     4741 gtgtcggaag tttgatgagc cgactcggtg ttcgagtagg tgagtctatg cctctatatg
     4801 agcggcttca gattcaaatg gaaatcgcaa agcatataga gctacttacg cgtggtgttg
     4861 aaggctttgt tatcgatcgc acacctgctg atgttatggc ctacacgttg gatttggtcg
     4921 gccataccaa tgaagatcga tgtattgagt tagccctcga tatcgaaaag ttttgccaca
     4981 aaactgctat ttcaaacttt aacgccattg ctggcctacg cccgggagtc gctctctcag
     5041 agcgagatta cttgcgatca caacgagcat cattagaccg tctgtatgtc gctcgtattg
     5101 atgcgttgat gtgcggggaa ctgacaaaaa ttcacctgca tccgcaaagg ggggatctgc
     5161 aaaccttcgt cgtttccaac cgatatcgca cggttgaagc aagagccaga tcagtgatga
     5221 gaatgctaga taacgctgta gaaaagatag aaaaccggtt ctgtggtcga gtgaccgttc
     5281 attagaaatt gttcgcctct tcgacattgc gacaataaaa ctctcaaaat gggttaagga
     5341 taaaaaatgt ttagtgaaat gttgcttgaa gatgaactgg atcggaaaac aacagaggct
     5401 ttgattcgtg tagcggacga acattcccgg tcgcttatga gcgatcgaga ggctcgtctg
     5461 gctattcgtg ccatattcga aactgcgcag gggcttgttg gcgcacaagt aggtgaagcc
     5521 attaacatcg ccatgtctca gttcagtgaa gacagtaaaa agcctctgtt tcctatgcat
     5581 ttgatgctgg ctggtggcac ggtgctttat atctctgttt gtctggatag caaccaaatc
     5641 aatattctca acactgcgtc aggtaagtgg aaagatccga ttgtctgtga aaccagtgaa
     5701 gaaactttga aaaaagcggc tcaatttgta cgtagcgcac tacttaaggg cgctaagaag
     5761 ttgtaaggag ttctgatgac aacgattgtt gcaggcatcg atatcgagtc tacgggactg
     5821 gatttccttg ctggtcataa aattattgaa atcgcaatta cccgctatga actggagaca
     5881 cagagacata ttgatagtct ggagatgcgt tttaaccctc gcagaaacat agatccgaaa
     5941 gctcaagccg ttcatggcat ttcattagaa cagctcgcag ctgaaccttt gttgtcaaat
     6001 catgctagcg aaattggcgc ttatatggag gcatgtagtg cgtgggttgc tcataacggc
     6061 gaagcgtttg atataccatt tattagacac gagttttcag ggtatggagt aagactgcca
     6121 gacgttcccg ttatagatac tatgttatcg ggattgtggg ccacagaaga cggtaaacgt
     6181 cctcgccttg aagaattggc cttctctctt ggctttatat acgatcatgc caaagcacat
     6241 agtgccttat atgacacaaa cttaatgatg caatgcttct ttaaggcacg taataaatac
     6301 ggatttttta aattaccctc tgaaattgtg taaaacaaaa gcctgcttta aagagagttt
     6361 gaagcaggct ttcttttaaa gaacagtcgc ctttcaatca tttcctgcct gtatttaata
     6421 cttttccgcc tgataggttt agtcaaaatg tagccatcga aacgcaaatg caactaaaca
     6481 gaaggagact tacatgagtt cggttgaaaa tgtaatgaca aatgatgatc tggatgagct
     6541 gacagccatg ttgcaatcac ttgatgaacc agtaaaaaaa gctgcacagg ttgaaaatac
     6601 tgatgatatt gacgatctgc ttctcggcct cgatgctggc gtagccatga gttctgatga
     6661 tgttgccgaa gaactgttca atgaagaaaa agcaggtgat ttcagctctg ctttaaatga
     6721 gttggagtta gcgcatgagc ctataaacgt aattaacgct gaaagtggtg aagctgccga
     6781 aaatgagcca gaacagttgg ggatcattga ggttgaagat tgtgttgagg ttaatgatga
     6841 attaaaagtt caacagtcaa atgatagcaa tacaaataaa aaaactcgtg ctgcaagagg
     6901 tcctagtttt actttaagtg ataaagatga ttcgtttttc aataaagcag gcttagaaaa
     6961 agatattttc ttagacgctt acgaaaacgc gcctgtcaaa gcgaaggata agatattaaa
     7021 ccttcttaat tggtttagcg gaggtccaga tattagtgtt tacacggtaa tttccatgag
     7081 acaccttctt acagaaaaga aggctacaag taatagtatt aagattgctt taatgagcaa
     7141 tccagaaaaa ccgtatccgc ttaacactgc gtcaactcag gctgggcaaa tgatggctgt
     7201 atttccagct acaggaattg ccgttagaga cggtggaaat ctaacattga acgaagaatc
     7261 accgatcgtt aagaagtttg tcgcggagta cactattgga tgacgttccc ctactgaaaa
     7321 taaagcccat agagagcttt atagtgctgg gtaagataac ccatacccag caccaaaaaa
     7381 acgcgccaga gagcttcttg ttcgcatttc tggcgcgttt tatttgattg ccatacataa
     7441 aatcaaatgc aaaaataggt atttacttac ctatcgagaa agaagatgat tgcagccgaa
     7501 aaaatcaaac agcgaaagcg cgacaactct cttcgtgacc tctggagaac acctgattgg
     7561 ctgttttctg ccattcaacg ttatcttgga gtgacatttg atgttgacgt tgcctgcaac
     7621 aaggacaacg caaagctgcc taatttcata ggcattgagc gtgatgcttt gaaatctgaa
     7681 tggggacagc caggtacaat tgccttcctc aatccaccct actccaaaat ctccccctgg
     7741 attgatgctg ctatacgtga gcaggctcgc ggagttacta cagtgatgct aattcctcaa
     7801 tccctcgata caaagtggta tgagcgtgca acagagtatg cgaatgagac gattattctg
     7861 tctggtggcc gcgtagcgtt tgtcgagcct gacgtcaatt tgggtcaggt agaagtaaac
     7921 atcaaccccg gtggcagtat gctcgttgtt tttcgaggat tctgtcagga cgctgggcac
     7981 tctataagca agatcccttt ggacgtcatg aaaagtctgg gagggtatga tcctgcgaat
     8041 gtgatcagga aaaaaagacc atcaaagaag gctgcttagt ttgttctggc gtctgtaatt
     8101 agcctgcttc tgtatatata taaataacta catattaatt attaatatac ggaagcaggc
     8161 tgttttgtat cagagactcc agacctgaac atcactacag aatccactag acccccttcc
     8221 cagacgcttt aaaatcgatt ttatgaacca ctttaaggaa accaacatgt catacccgac
     8281 taatgtcgtt gcgctcgtag acagcgattt tctggcccag gctcgtgaaa tgatgaaaga
     8341 tcgtgagcag gctttcaact tgtacgagtg ggcaattaag tgcttgcatc ttggggagca
     8401 tcgcgaactt gttgaacagc ttttaggtga gttgatcaac gaggtgtttg ccttgaatgt
     8461 tcaactacat ggtcgagaaa ataatcaatc gaaatgatag ataagtacaa actattcata
     8521 agatgaattg taagtgctaa gatctgatag tttccagtcg tagactggag actcgacctg
     8581 atgggtgggg gtaagcgtca ctggcgtcag gtttaaaaaa gctcactacc agcgtagaac
     8641 cggtgccgtt taggtgtcgg ggaaggggga accaaagtga gcggagacaa gggtcacttt
     8701 atgattgtcg agtctggggt gtttcgagag gttgaatcca gtactcccct tcataaagtg
     8761 tgggaagatc tcggttctgg ggtgctgtca tccataactt cccaagtcta agctggcagt
     8821 agacttagac catagctttt caggttatga aacgaccagg ttggtgagga attttatact
     8881 cacctccctg ggagagtatt acctgaaaag acaacctctc acttcgttcg aggtgaactt
     8941 cactcacttc gttcgttcag ttcaggttta taaaaacctg ttctgggaag taatttgttt
     9001 attttaataa ttattaacac gcacgcgtgt gcgcacgcgc gaggaaaaat cggcgcggcg
     9061 cttgattcag gagtttatat gacaacgaag acaccagccc gaccgcaagc aaaaactcgc
     9121 aaaaaagaca aaaacaaaaa ttctccccgc accaatccca caacgcctgt cgtagagttc
     9181 aatccccagc ttaaaaccgt gaaaatcttt agtgatggct cttgccttaa aaatccgggt
     9241 ggcccgggcg gttacggtat cgttctccag tatcgtggcg aggaacgcga gttctcagat
     9301 ggttttcata gcaccaccaa taaccgcatg gagatgatgg gggcacttat cgggctggag
     9361 cgtttgaaat atccatgcaa cgttattttg cactctgata gccagtatct gaaaaacggc
     9421 atgacacagt ggatgaaatg gtggaaacgc aatggatgga tgacttctga caaaaaaccg
     9481 gtaaagaatg ttgatctgtg gaagcgtctg gatgaggccg caagtcgaca taatgttcgc
     9541 tggaagtggg ttaaaggtca cgccgggcat cgtgaaaatg aaatatgtga tcgactcgcg
     9601 aagatcgcag ctttttcagc
//
LOCUS       DAZOXR010000591         9522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.168, whole genome shotgun sequence.
ACCESSION   DAZOXR010000591 DAZOXR010000000
VERSION     DAZOXR010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.168"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHUNN_004916"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHUNN_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUNN_004917"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUNN_004917"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0579020.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUNN_004918"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUNN_004918"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0579021.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUNN_004919"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUNN_004919"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0579022.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUNN_004920"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUNN_004920"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0579023.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUNN_004921"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUNN_004921"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0579024.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHUNN_004922"
     CDS             5099..6232
                     /locus_tag="ACHUNN_004922"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0579025.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHUNN_004923"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHUNN_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0579026.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUNN_004924"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUNN_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0579027.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHUNN_004925"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHUNN_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0579028.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHUNN_004926"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHUNN_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0579029.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHUNN_004927"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHUNN_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0579030.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="ACHUNN_004928"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="ACHUNN_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0579031.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2413 a         2446 c         2448 g         2215 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggt ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccatccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cg
//
LOCUS       DAZOXR010000592         7705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.202, whole genome shotgun sequence.
ACCESSION   DAZOXR010000592 DAZOXR010000000
VERSION     DAZOXR010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.202"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            378..536
                     /locus_tag="ACHUNN_004929"
     CDS             378..536
                     /locus_tag="ACHUNN_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0579032.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            996..1811
                     /gene="sul2"
                     /locus_tag="ACHUNN_004930"
     CDS             996..1811
                     /gene="sul2"
                     /locus_tag="ACHUNN_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0579033.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1872..2675
                     /locus_tag="ACHUNN_004931"
                     /note="aph(3'')-Ib"
     CDS             1872..2675
                     /locus_tag="ACHUNN_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR0579034.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2675..3511
                     /locus_tag="ACHUNN_004932"
     CDS             2675..3511
                     /locus_tag="ACHUNN_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR0579035.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3817..4059
                     /locus_tag="ACHUNN_004933"
     CDS             3817..4059
                     /locus_tag="ACHUNN_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR0579036.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="ACHUNN_004934"
     CDS             complement(4091..4741)
                     /gene="tetR(A)"
                     /locus_tag="ACHUNN_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR0579037.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4847..6046
                     /gene="tet(A)"
                     /locus_tag="ACHUNN_004935"
     CDS             4847..6046
                     /gene="tet(A)"
                     /locus_tag="ACHUNN_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR0579038.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(6013..6201)
                     /locus_tag="ACHUNN_004936"
                     /pseudo
     CDS             complement(6013..6201)
                     /locus_tag="ACHUNN_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6245..6634)
                     /locus_tag="ACHUNN_004937"
     CDS             complement(6245..6634)
                     /locus_tag="ACHUNN_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0579039.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6852..7292
                     /locus_tag="ACHUNN_004938"
     CDS             6852..7292
                     /locus_tag="ACHUNN_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0579040.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(7351..>7705)
                     /locus_tag="ACHUNN_004939"
     CDS             complement(7351..>7705)
                     /locus_tag="ACHUNN_004939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0579041.1"
                     /translation="TSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAP
                     ATLHGHAINCVPVHRFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVF
                     GWFVACFTGFPSETP"
BASE COUNT         1641 a         2187 c         2155 g         1722 t
ORIGIN      
        1 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
       61 cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag
      121 ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa
      181 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc
      241 cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
      301 cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc
      361 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg
      421 gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca
      481 ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc
      541 ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa
      601 gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt
      661 acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact
      721 atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta
      781 acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc
      841 tacttattga gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg
      901 gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag
      961 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
     1021 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
     1081 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
     1141 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
     1201 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
     1261 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
     1321 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
     1381 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
     1441 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
     1501 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
     1561 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
     1621 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
     1681 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
     1741 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     1801 gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc agaaaactga
     1861 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
     1921 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
     1981 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
     2041 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
     2101 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
     2161 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
     2221 ttgatcaatg tcagtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
     2281 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
     2341 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
     2401 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
     2461 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
     2521 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
     2581 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
     2641 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
     2701 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
     2761 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
     2821 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     2881 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     2941 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     3001 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     3061 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     3121 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     3181 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     3241 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     3301 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     3361 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     3421 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     3481 gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct atcccctggg
     3541 aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg attccgcccc
     3601 ttcccgcaaa cgacccctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag
     3661 ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg
     3721 cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt
     3781 ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc cctggacctc
     3841 gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg
     3901 acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg
     3961 acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg
     4021 aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca
     4081 gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc
     4141 ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg
     4201 cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg
     4261 tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag
     4321 tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc
     4381 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
     4441 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
     4501 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
     4561 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
     4621 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
     4681 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
     4741 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
     4801 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacag
     4861 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
     4921 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
     4981 tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc
     5041 gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt
     5101 cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg ggcggatcgt
     5161 ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg atatcactga
     5221 tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt tcgggatggt
     5281 cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc cgttcttcgc
     5341 cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc cggagtcgca
     5401 caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt cgttccggtg
     5461 ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca tgcaacttgt
     5521 cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc actgggacgc
     5581 gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg cccaggcaat
     5641 gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat
     5701 tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga tggcgttccc
     5761 gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag caatgttgtc
     5821 caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg cgctcaccag
     5881 cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt ctataacaac
     5941 gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct
     6001 gcgtcgcggg ctttggagaa attcttcaaa ttcccgttgc acatagcccg gcaattcctt
     6061 tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga tctgatagag
     6121 aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc ggctggccgt
     6181 cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac atacagtcta
     6241 tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct agacattgcc
     6301 agccagtgcc cgtcactccc actcttacat tgttcgcagt atctaggtac atgttattgc
     6361 gtgcaccttc atgatgtata atgttattaa tgacagcaga aagatcgaca gaattaatat
     6421 tcttcgtacc tgggacagtt atcgggtgtc ggccagtgat gagaccaaag gatgcagtat
     6481 aagatgaacc actactatta tcatggccac cattaccacc accgttaaaa ctacctcccg
     6541 aacttccaga gccgctatga ccatcattgt aggggtcatt tggtcccatg tgaccaaaac
     6601 caccgacagt accgctattt aactctgtac tcataaaatt atcctctttg acttttaaaa
     6661 acagcaaaaa ctgtatttat aacaatgtgt ttatatacag tattcgaaat actacgcccg
     6721 tcaaaaccac accgtcaaca ggttttttgt gtatttttca cgcctcacgg gcagcctcag
     6781 accttttgtg ttaaaacagt aatatgttct gctgttacaa tacggtacac acaggagaca
     6841 ctgtcacttt tatggcatgg cacacagttc aagagacact gaaattaaca ggaaagtcgc
     6901 gctcgcaatt atatcgtgac atggctaaag gccttgtcag ctatcgcaca ggaaaaaatg
     6961 atcgccggga gttcgagacc agtgaactga ttcgggccta tggcgagctg cgcacttctg
     7021 agacaccaaa aaggcacaag gagggacact ccaaatctgt cgttagccag gcggaaaatg
     7081 ctcagctaga cgtcattcag cagaaactgg acgctctgca gcagactgtt accctaatgc
     7141 ttgaagacaa gatggcgcgg gaagcagaaa accgcctgcg aaatgaggag agggaacaac
     7201 tacaggccga aattagccgt ctggctgacg cgctggcaca ggaaaaaaag cggggattct
     7261 ggtcgcggct gtttcgctcc ggttcagagt aacagtgcag gtgactctga tttaccataa
     7321 catctgttat cagcaccgcc tgagaggccc tcagggcgtt tctgacggga aaccggtaaa
     7381 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     7441 catgagtatg cgcacaagtg catagcgcgg aaattcacac cattcagccg gggtaagtcc
     7501 tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     7561 gtgaagcgtt gccggcgcag ccggaaaacc cgctgtcagc acgttcagga agggggtgat
     7621 gtacaaatgg cacagaggat gaaagtgacg tatgagctga aggaatgatg taaaagtgat
     7681 gtataagtga tgtaaaagtg atgta
//
LOCUS       DAZOXR010000593         1813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.372, whole genome shotgun sequence.
ACCESSION   DAZOXR010000593 DAZOXR010000000
VERSION     DAZOXR010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.372"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            112..207
                     /locus_tag="ACHUNN_004940"
     CDS             112..207
                     /locus_tag="ACHUNN_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFR0579042.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHUNN_004941"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="ACHUNN_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFR0579043.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          663 a          303 c          295 g          552 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttggt gatgctgcca acttactgat
     1801 ttagtgtatg atg
//
LOCUS       DAZOXR010000594         1704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1905222
            SAMN43277365-rid23486713.guided.377, whole genome shotgun sequence.
ACCESSION   DAZOXR010000594 DAZOXR010000000
VERSION     DAZOXR010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277365
            Sequence Read Archive: SRR30464593
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396944.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396944.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:07:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,943
            CDSs (total)                      :: 4,842
            Genes (coding)                    :: 4,539
            CDSs (with protein)               :: 4,539
            Genes (RNA)                       :: 101
            rRNAs                             :: 1, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 5 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 303
            CDSs (without protein)            :: 303
            Pseudo Genes (ambiguous residues) :: 0 of 303
            Pseudo Genes (frameshifted)       :: 132 of 303
            Pseudo Genes (incomplete)         :: 108 of 303
            Pseudo Genes (internal stop)      :: 97 of 303
            Pseudo Genes (multiple problems)  :: 33 of 303
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277365-rid23486713.guided.377"
                     /strain="M1905222"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-05-08"
                     /collected_by="FSS"
     gene            <1..474
                     /locus_tag="ACHUNN_004942"
     CDS             <1..474
                     /locus_tag="ACHUNN_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0579044.1"
                     /translation="DDKFITTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRD
                     TADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGR
                     QEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="ACHUNN_004943"
     CDS             657..1517
                     /locus_tag="ACHUNN_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0579045.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          455 a          374 c          437 g          438 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgc
//
